LOCUS       DATMXK010000001         2814 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_90594, whole genome shotgun sequence.
ACCESSION   DATMXK010000001 DATMXK010000000
VERSION     DATMXK010000001.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2814)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2814)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2814
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_90594"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1989
                     /locus_tag="VN363_00005"
     CDS             <1..1989
                     /locus_tag="VN363_00005"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWQ82912.1"
                     /translation="FSGSHRFLVEYLMDEVVNRQSQEVQTFLLSTSILERMCAPLSDA
                     LPDATPHSEAILKLLEQANLFVVALDDQGNWFRYHRLFRDFLQTRLNKTQPERINRLH
                     RAACEWLAAHANLREAASHAFQTGDWEYAAAFVEQHSFDLIVHSDISTIYEWCAVFPE
                     EVMQRHPMLSLQQCLALAYGFRRKNRVRIEARLQQVDQLILTMEDRQQAHILIDLAAV
                     VRTFQAFAPDLAADPRELLSLAQHMLNNYSEADPARFTGLLLTGYTYLALHDAIAAGQ
                     AFEIARQIASHERLYFGIVESTFHLARLAHSQGQLRRAAEISRQGKADIATVLAHPEQ
                     ELPALGCLEVALGCVLLEQDCIDEAEAYLRHGLDLMGGGMNPYYLMTAYVALFRLSEI
                     KDRQEEAFKSLDHLEAAWPDIAFCTRGLRVRYLLRSAPDDQRSLAEAAAWCQEISSSL
                     EENVSLPGLGPFGGGEAYYLAYLTWIRAQIAFGKPQVVTSYLTRQLDLAEAHGLTTRV
                     IEVSLLEALAAQAEGDHHRSFAALERALSAAQPEGYVHIFDQGPALTQLLVEAARRGI
                     FKEYIERILAAIGMPKAFELERGVAAQTPYGESLSERELDVLRLIAQGYTNQEIAKQL
                     VITVGTVKSHINHIFGKLDAHNRTEAVARARGLGLIEI"
BASE COUNT          622 a          787 c          777 g          628 t
ORIGIN      
        1 ttctcgggca gccaccgttt tttggttgaa tacctgatgg atgaggtggt caatcgccaa
       61 tcacaagagg tgcagacgtt tcttctctcg acatccatcc tcgaacgcat gtgcgcccct
      121 ctaagcgatg ccttgccaga cgctactccc catagcgaag cgattctcaa gctattagaa
      181 caggcaaacc tgtttgtggt ggccctcgat gaccagggaa attggtttcg ctatcaccgc
      241 ctctttcgcg acttcctaca aacccggctc aacaaaaccc agccggagcg tataaacagg
      301 ctgcaccggg cagcctgcga gtggctggca gctcatgcaa acttgcgcga agcagcatcg
      361 catgcttttc aaaccggcga ctgggagtat gcggcggcct ttgtcgaaca gcacagcttc
      421 gacctgatcg ttcacagtga catctcgacc atatatgaat ggtgtgcagt ctttcctgaa
      481 gaggtgatgc aaaggcatcc aatgctcagt ctccaacagt gccttgcatt ggcttatggt
      541 tttcggcgaa agaaccgggt caggatcgaa gcccgcttgc agcaggtcga tcaattgatc
      601 cttacgatgg aagacaggca gcaggcgcat atcctgatcg atcttgctgc agtcgtccgt
      661 accttccagg cctttgcccc agacctggct gccgacccgc gagagctgct ctccctcgct
      721 caacatatgc tgaacaatta ttccgaggct gatccagccc ggtttaccgg gctgcttctg
      781 accggctaca cttaccttgc gcttcacgac gccatcgcag ccggtcaagc ctttgaaata
      841 gctaggcaga tcgcctcgca tgagaggtta tattttggga tcgtggagtc aactttccac
      901 ttggctcgac ttgctcacag tcaagggcaa ttgcggagag ccgcagagat cagccgccag
      961 ggaaaggcag acattgcaac cgtgctggcc caccccgagc aagaactacc cgccctgggc
     1021 tgcctggagg ttgccctggg ctgcgtgtta ctggaacagg attgtattga tgaagcagaa
     1081 gcatacctcc gtcatggcct ggacctgatg ggcgggggaa tgaaccccta ttacctgatg
     1141 acggcatacg tggcgttgtt tcgcttgagc gagatcaagg atcgacagga ggaggctttt
     1201 aaatccctcg atcacctgga agccgcctgg cctgatattg ccttttgcac gcgcgggttg
     1261 cgggtcaggt atctcttgcg ttctgcgccg gatgatcaga gatcgctggc agaagccgct
     1321 gcctggtgtc aggaaatctc ttcctctctc gaagaaaatg taagcttgcc cgggctgggt
     1381 ccttttgggg gtggcgaagc gtattatctt gcctatctca cctggatccg cgcccagatt
     1441 gcattcggca aaccgcaggt agttacgtct tacctcaccc ggcagctcga tctggccgag
     1501 gcgcatggcc tgacgacccg tgtgattgag gtctctctac tggaggcgct ggctgcgcaa
     1561 gccgaaggcg atcaccaccg cagctttgcg gcgctcgagc gtgcgctttc agccgcgcag
     1621 cccgaaggct atgtccatat ctttgaccag ggaccagctc tgactcaact gcttgttgag
     1681 gcagcccggc gcggcatctt caaagagtac atcgagcgta tattagccgc tataggaatg
     1741 ccgaaggcct ttgagctgga acggggggtc gccgctcaga cgccctatgg tgaaagcctg
     1801 agcgaacgcg agctggatgt gttgcgcctc atcgctcagg ggtatactaa ccaggagatc
     1861 gcaaaacagc tcgtgatcac tgtcgggaca gtgaaaagcc acatcaacca tattttcggg
     1921 aagctggacg cccataaccg gaccgaagcc gtggcacgcg cgcgaggatt gggcttgatc
     1981 gaaatctaac cttaaatcta acttttggta gatgactgca gactggctta ccgttatgct
     2041 agttctatgc tggattatgc ccgacaacgt gcactagaag cactgaggac ccatcgcaaa
     2101 gctgttttag cgacgagtgg gcctgctggc attcaggcag gcgagtttcc ctgcgaagca
     2161 attgacctcg acctatatct tttggtgcca aaaacctccg atcacctgtt caacctggag
     2221 catgattcca ccgtaacctt gctaacagcc aggtgggaac tgaaaggaca ggctcacatt
     2281 gtcccgcccg gaacatcaga tctcaagtta gaccttttac tcggaccagg ggcagaatgg
     2341 tgtgtgctct tgcatgttga tccttgccgg ttacaaattc gaagggatgg aggctggggg
     2401 aaccttgaga ccatcgacct caaatcctat tgatgggaat ggccgaacta tcgaatcaaa
     2461 tctcaaacag gcaaaggcta ctgcttcatc cctctttcga caggtcgatc cgaccctggc
     2521 gcaaagctgc cagcccttcg tggcttggcg gctggagtgg gtcagcagcg atattgagcg
     2581 gctgtggcca gaaggcatct cgtagatctg caacgaacat ggctcagctt acgtcacggg
     2641 ttacgcccac gacgacgccg gcaacctgat ttacctgggc atggtcgggc ataagacggt
     2701 gctggagtca atccgagcga ctgtccaggc aaggcaacgc aggaagcttt tcctgcaagg
     2761 gaggctagtc tacccgcttg cctctcacta atgccagacc tggcagcatc tgcc
//
LOCUS       DATMXK010000002         4864 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_147824, whole genome shotgun sequence.
ACCESSION   DATMXK010000002 DATMXK010000000
VERSION     DATMXK010000002.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4864)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4864)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4864
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147824"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..594)
                     /locus_tag="VN363_00010"
     CDS             complement(<1..594)
                     /locus_tag="VN363_00010"
                     /EC_number="2.7.4.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559450.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016776 - phosphotransferase activity,
                     phosphate group as acceptor [Evidence IEA];
                     GO_function: GO:0019205 - nucleobase-containing compound
                     kinase activity [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate kinase"
                     /protein_id="HWQ82913.1"
                     /translation="MPVYIVLVGPPGAGKGTQAQAISAKLGLPHISSGDIFRENLKLQ
                     TELGQQAAAYMNRGELVPDDLTIAMIQERLSRPDCVPGALLDGFPRTPAQAQALNEML
                     GKFGGSVNAVPYINVPEDVLVERLTGRWTCRAAGHVYHEKFNPSQVLGVCDLDASELY
                     QRDDDKAETVTRRIRVYLEQTAPLIAYYQDAGVLIEVD"
     gene            complement(605..1942)
                     /gene="secY"
                     /locus_tag="VN363_00015"
     CDS             complement(605..1942)
                     /gene="secY"
                     /locus_tag="VN363_00015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00967.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecY"
                     /protein_id="HWQ82914.1"
                     /translation="MKRSAWRYLWSAQDIRRKLLITLGILAIYRLAAHVPVPGADRDV
                     IAGILQGGGAQGTLVGILDLLSGGTVSNFSVLAMGVYPYITAQIILQLLVPIIPALQK
                     RMEDDPREGRKWMEKWTYYLAVPMAVLQAIGQINIFSSFAGQPIIENFGFSGANIVPS
                     LTIVLSMTAGTMFAIWLGELISEFGIRNQGLSLIIFAGIVTQIPTNIVTLWADEQRRF
                     ITIPILLIMMILIIFAIVYVQQGRRNVPVMYPGRRMGNRMSMPVKGTLPLMVNMAGMI
                     PLIFAQSLLTFPAIIASFFTQSTSEWLKNAAVSIQNLFGGQGAVYYILYFLMVVGFTF
                     FYTNVLFTQQNYGENLKRVGAQIPGVSRGAPTQKYLTKVLGRITLPGALFLGIVAIFP
                     WLVGLIVPGLGQNSTLLVSSAGLLIVVGVVRDTFFNIEAELKLHGYDDALLVR"
     gene            complement(1964..2422)
                     /gene="rplO"
                     /locus_tag="VN363_00020"
     CDS             complement(1964..2422)
                     /gene="rplO"
                     /locus_tag="VN363_00020"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016911111.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L15"
                     /protein_id="HWQ82915.1"
                     /translation="MKLHDLKPEEGSKKNRKRVGRGISAGQGKTAGRGTKGQGARSGG
                     PARLYHQGGNLPFFRRLPFKRGEGFTPINRVEYNEINLDQLTDFKAGQEVSPTSLTGA
                     RLLRDPRNPVAVLGRGDVNVALKVSAHRVTKAAQAKIEAAGGSVEILELG"
     gene            complement(2431..2634)
                     /gene="rpmD"
                     /locus_tag="VN363_00025"
     CDS             complement(2431..2634)
                     /gene="rpmD"
                     /locus_tag="VN363_00025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006640333.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L30"
                     /protein_id="HWQ82916.1"
                     /translation="MANALKNTAKVVRVKLVKSPIGYAKQQKATVRALGLTKMLQTVE
                     HVDSAALRGMLAKVAHLVAVEEE"
     gene            complement(2634..3158)
                     /gene="rpsE"
                     /locus_tag="VN363_00030"
     CDS             complement(2634..3158)
                     /gene="rpsE"
                     /locus_tag="VN363_00030"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_018356922.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S5"
                     /protein_id="HWQ82917.1"
                     /translation="MSDYEFSEFQQPEFDERVVDIARVAKVVKGGRRFSFRVTVVVGD
                     NRGSVGIGIGKANAVPDAMRKASEQARRNIHKVSLFKTTVPHEVIGKVSGARVLLKPA
                     SAGTGVIAGGGVRAVLEAAGVTDILTKSLGSSNILNVVLATMDALDQLKSPQDEAARR
                     GKPVKDLMPFWERK"
     gene            complement(3212..3580)
                     /gene="rplR"
                     /locus_tag="VN363_00035"
     CDS             complement(3212..3580)
                     /gene="rplR"
                     /locus_tag="VN363_00035"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_015302640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L18"
                     /protein_id="HWQ82918.1"
                     /translation="MANKKSRSVARERRHIRVRKNLAGTTERPRLNVYRSTAEIYAQV
                     IDDVVGSTLVSASSVDHELRTRMAGLKKSEQARLVGEAIAKRALEKGIKTVVFDRGGF
                     RYQGRVKELAEAARKGGLEF"
     gene            complement(3585..4127)
                     /gene="rplF"
                     /locus_tag="VN363_00040"
     CDS             complement(3585..4127)
                     /gene="rplF"
                     /locus_tag="VN363_00040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008372550.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L6"
                     /protein_id="HWQ82919.1"
                     /translation="MSRIGRMPVVIPKGVEVDIQGSHVHVKGPNGELSRVFPAAISIV
                     FEEGNIEVRRSSDDRTSRALHGMTRALINNMVVGVSSGFDKLLEVNGVGYRAEMSGNN
                     LMLHVGYSHPVTFEPPEGIRFETDARTRQIKVIGRNKEQVGQIAADIRKVRPPEPYKG
                     KGIKYLDEKIRRKAGKSGKV"
     gene            complement(4204..4617)
                     /gene="rpsH"
                     /locus_tag="VN363_00045"
     CDS             complement(4204..4617)
                     /gene="rpsH"
                     /locus_tag="VN363_00045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002857494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S8"
                     /protein_id="HWQ82920.1"
                     /translation="MSTTDPISDMLTRVRNAVMAGHTMVALPSSKIKVAIAKIMKEEG
                     YITNFEVVDGKIPAQKVLRIRLKYVGERRERRPVITGLERISRPGRRVYSGKQEIPWV
                     LSGMGIAIISTPKGVMTGQRARQMGVGGEVLCKVW"
     gene            complement(4637..4819)
                     /locus_tag="VN363_00050"
     CDS             complement(4637..4819)
                     /locus_tag="VN363_00050"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003948630.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type Z 30S ribosomal protein S14"
                     /protein_id="HWQ82921.1"
                     /translation="MAKKCMTIRETRRKFDVRVRNRCKVCGRPRGYIRRFGLCRICFR
                     ELALEGKIPGVVKSSW"
BASE COUNT         1148 a         1414 c         1183 g         1119 t
ORIGIN      
        1 atccacttcg atcaaaacac ctgcgtcctg atagtatgcg atcaagggtg cagtttgttc
       61 gagatagacg cggatccggc gtgtcactgt ctcggcttta tcatcgtccc gttggtaaag
      121 ctccgaggcg tccaggtcac agacgcccag aacttgcgac ggattgaatt tttcgtgata
      181 tacatgcccg gctgcccggc atgtccaacg tccggttaac cgctccacca acacatcttc
      241 ggggacattg atgtacggca cggcgtttac agatccaccg aatttaccca acatttcatt
      301 gagcgcttgc gcctgggccg gcgtgcgagg gaagccatcc aacaaagctc ctggtacgca
      361 gtcggggcga gataggcgtt cctggatcat ggcaatcgtc agatcatccg ggaccagttc
      421 gccgcgattc atatacgcag cagcctgctg gccaagctcg gtttgtagtt ttaaattctc
      481 ccgaaagata tctcccgagg agatgtgcgg taagcctaac tttgctgaaa ttgcctgggc
      541 ttgagtgcct tttccggcgc ctggaggtcc aactaagaca atatacactg gcatccgtct
      601 gtccttaccg caccagcaag gcatcatcgt agccgtgcag cttcagctcg gcttcgatat
      661 tgaagaacgt atctcttacc acgccgacga cgatcagcaa accggcggag gagaccagca
      721 aagtgctgtt ttgacccaat ccggggacaa tcaacccgac cagccatgga aagattgcta
      781 cgatgcccag gaataaagct cccgggagcg taatgcggcc caaaactttg gtaaggtact
      841 tttgggttgg agcaccccgg ctgacgccag ggatttgagc acccacgcgt tttagatttt
      901 ccccatagtt ttgctgagta aaaagcacgt tcgtataaaa gaacgtaaac cccacaacca
      961 tcaggaaata cagaatatag tagacggcgc cctggccacc aaacaggttc tggatggata
     1021 ccgccgcgtt tttcaaccat tcagatgtcg attgggtaaa gaaactggca atgatcgccg
     1081 ggaaagtcag cagcgactgg gcaaagatca gcgggatcat gcctgccata ttgaccatca
     1141 ggggcagggt gcccttgact ggcatagaca tccggttgcc catccggcgt cccgggtaca
     1201 ttactggcac gttgcgccgt ccctgctgca cataaacaat cgcgaagatg atcaatatca
     1261 tcatgatcag caagatcggg atagtaatga accggcgctg ttcatccgcc cagagcgtga
     1321 caatattcgt cggaatctga gtcacgatgc cagcgaagat gatgagggaa aggccctggt
     1381 tgcggatccc gaattcggag atcagctcac ccagccagat cgcgaacatc gtccctgcag
     1441 tcatcgagag cacaatggtc agtgagggta cgatattcgc cccactaaag ccaaagtttt
     1501 cgataatcgg ctggccggca aacgatgaga agatattgat ctgaccaatt gcctggagca
     1561 ctgccatcgg cactgccaga taataagtcc acttttccat ccatttacgg ccttcgcggg
     1621 gatcatcctc catccgtttc tgcagggccg ggataatcgg caccaacagc tggaggataa
     1681 tctgggcagt aatataggga taaacaccca tcgccagcac ggagaaattg gataccgtgc
     1741 ctccggagag taaatccagg attccaacca gagtaccttg ggccccaccg ccttgtagaa
     1801 ttccggcaat tacgtctcga tcagcgccag gcacaggtac atgtgctgcc aggcggtaga
     1861 tcgccaggat ccccaacgtg atgagcagct tccggcgaat atcctgtgcc gaccacaggt
     1921 agcgccaagc agaccgcttc atagcctcaa ctccttgtca ggactaaccc agttccagga
     1981 tttcaacact gccaccggca gcttcaatct tggcctgagc agccttggtc acacgatgag
     2041 cgctcacttt aagcgcgaca ttgacgtcac cacgccccag tactgcaacc gggttgcgag
     2101 gatcgcgcag caagcgtgcg ccggtcaaac ttgttgggct gacttcctga ccagctttga
     2161 aatcagtgag ctgatccaga ttgatctcgt tatattcaac acgattgatc ggggtaaacc
     2221 cttcaccacg tttgaacggt aagcggcgaa agaacggtaa gttaccgccc tgatggtaca
     2281 ggcgagcagg accgccagag cgtgcaccct ggcctttggt tccccgacca gccgtcttgc
     2341 cctggcccgc agagatccca cgcccaaccc gtttccggtt tttcttcgat ccttcttcag
     2401 gtttaagatc atgaagtttc atgtgcttac ctattcttct tccacggcca ccagatgggc
     2461 gaccttagcc agcattccac gcagtgcggc ggaatcgaca tgctccacag tctgaagcat
     2521 cttggttagc cccagagccc gcacagtggc tttttgctgc ttggcgtagc cgatcgggct
     2581 cttaaccagc tttacccgga caacttttgc ggtatttttt aatgcgttcg ccattactta
     2641 cgctcccaga acggcattag atccttgaca ggcttgccac gccgggcagc ttcatcttgc
     2701 ggcgatttaa gctggtccag cgcatccatg gtagccagta cgacattcaa aatattactg
     2761 ctgcctaagg atttggtcag aatgtctgtc acgccagcag cttcaagcac tgcccgcaca
     2821 ccaccgccgg cgataacacc cgtaccagcc gaagctggtt tgagcagcac tcgtgcgcca
     2881 gagaccttgc cgattacttc atgtggcaca gtagtcttga aaagcgagac tttgtgaata
     2941 ttgcggcggg cctgctcgga tgccttacgc atcgcatcgg gcacagcatt ggctttgcca
     3001 atcccaatcc ctacgctgcc acgattatca cccaccacaa ccgttacccg gaaggaaaag
     3061 cggcgaccgc ccttgacgac tttggcaacc cgggcaatat ccacaacacg ctcatcaaat
     3121 tcaggttgtt gaaattccga aaactcgtag tctgacatat ctctctcctg gtaagagtgt
     3181 tcttcagcga tcgccgctga ttaactctta cttagaactc caggccgcct ttgcgagctg
     3241 cttcagccaa ctctttcacc cgaccctgat aacggaaacc accccggtca aagacgaccg
     3301 ttttgattcc tttttccaga gcgcgctttg cgatcgcctc acccaccagg cgtgcctgct
     3361 cacttttctt cagtccagcc atccgggtac gcagttcgtg atcgaccgag gaggctgaca
     3421 ccagggtact gcccaccaca tcatcgatta cctgcgcata aatttccgca gtactgcgat
     3481 aaacatttaa tcgtggtcgt tcagtcgtgc cagccaaatt cttgcgaact cggatatgcc
     3541 ggcgttcacg cgccacggaa cgagatttct tgttagccat aagcctacac cttaccagac
     3601 ttaccagcct tgcggcggat tttctcgtct aagtatttga tacccttgcc cttataaggc
     3661 tcgggagggc gaaccttgcg aatatctgca gcgatctgcc cgacctgttc tttgttacgt
     3721 ccgatcacct tgatctgacg cgtgcgggca tccgtttcaa agcggatccc ttccggtggc
     3781 tcgaaagtta ctgggtggga gtaacccaca tgcagcatta aattattgcc actcatctcg
     3841 gcgcggtaac ccacaccgtt gacttccaga agcttatcaa aaccagagct cacacccacg
     3901 accatattat tgatcagagc acgggtcatc ccatggagtg cacgagatgt acgatcatcg
     3961 gacgaacgac ggacctcaat attgccttct tcgaaaacaa ttgagatagc cgcgggaaac
     4021 acccggctta actcgccgtt cggacctttg acgtgtacgt gagatccctg gatatccact
     4081 tccacgcctt tgggtattac taccggcatg cgaccaattc tagacacttt gatcaacctc
     4141 ctgcatggtt tggcgctggc tgggaatcac tccatcaaac gcctatcacc tgcaataaat
     4201 ggactaccag accttgcaga ggacctcgcc tcccacgccc atctggcgcg cgcgctgacc
     4261 ggtcatcacg cccttcggcg tggaaataat cgcaataccc attcccgaaa gcacccaggg
     4321 aatttcctgt ttgccagaat acactcgccg tccgggacgg ctgatgcgct cgaggccagt
     4381 tatcactggg cggcgctctc gccgttcacc aacgtatttc aagcgaatgc gcaatacttt
     4441 ttgggcagga attttgccat ccaccacttc aaagttggtg atatagccct cttccttcat
     4501 gatttttgcg atcgcaactt taatctttga gctgggcagg gcaaccatgg tatggccagc
     4561 catcaccgca tttcgtactc gggtcaacat atcgctgatc ggatcagtgg tactcatcta
     4621 taatccctcc gcccggctac caggaagatt taacgacacc tggaattttg ccttccaggg
     4681 caagctcgcg gaagcaaatc cggcacaatc caaagcgacg gatgtagcca cgtgggcgac
     4741 cgcagacttt gcatcggttg cggacgcgga catcaaattt ccgccgtgtt tcgcgaatag
     4801 tcatgcactt ttttgccatt gattaacctt cctttgaaaa tggcatgccg agcatctgta
     4861 ataa
//
LOCUS       DATMXK010000003         3818 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_22673, whole genome shotgun sequence.
ACCESSION   DATMXK010000003 DATMXK010000000
VERSION     DATMXK010000003.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3818)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3818)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3818
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22673"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..232)
                     /locus_tag="VN363_00055"
     CDS             complement(<1..232)
                     /locus_tag="VN363_00055"
                     /inference="COORDINATES: protein motif:HMM:NF018984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABATE domain-containing protein"
                     /protein_id="HWQ82922.1"
                     /translation="MIMIEHFSGNNPLTPNLLGLDFANTLSSRGSDQPSDRLLQYTGL
                     LEWSEQIGLLRPVEAQTLANRARKDPEKALQVY"
     gene            381..656
                     /locus_tag="VN363_00060"
     CDS             381..656
                     /locus_tag="VN363_00060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82923.1"
                     /translation="MDNELQVLSEARYRYENYLKEADHERLAHEARLNYLTHPNRPFS
                     NTIPTRLLVWLGKHMTVWGAFLVERYDPGRVNSNRQLNHNIQTTHGC"
     gene            801..1574
                     /locus_tag="VN363_00065"
     CDS             801..1574
                     /locus_tag="VN363_00065"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:936..938,aa:Sec)
                     /transl_table=11
                     /product="redoxin domain-containing protein"
                     /protein_id="HWQ82924.1"
                     /translation="MALQVGDIAPDFCLDSHLGHPVRLSDYRGKRNVVLAFFPLAWTP
                     IUSGQIPSYQVALDRFDGVRTQVLGLSVDSVPSLIAWAESLTGITYPLLSDFYPHGKV
                     AETYGVLRSEGKSERALFIIDKQGVIRYVDVHDIDHQPENEVLFAELEKIEPYYAAQY
                     AARIAAQQKPEPQMISDSKADVILYCTPWCPGCRRARAFLRENQISFEEINIMQDRAA
                     AAQVREWAGGYETTPTLNVRGTIVIEFDLNRVAKLLGIK"
     gene            1571..1981
                     /locus_tag="VN363_00070"
     CDS             1571..1981
                     /locus_tag="VN363_00070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82925.1"
                     /translation="MNQEKSTLSSKNTGARRIQRLAAGLLILVLFAAAYLNSFYSPIS
                     ALADAGGWPSPTFVIIPTINFPTATLFPTVDLSNLNQLLITEPTPTATLFIVPTPGSP
                     ESRSALSYLCLPLGVGILLILILVSLMVMRRRQE"
     gene            2021..3640
                     /locus_tag="VN363_00075"
     CDS             2021..3640
                     /locus_tag="VN363_00075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82926.1"
                     /translation="MNGRLTVVAALFFFGVGSLFFQPWNTLLGLASDRAEQPLSTPER
                     IEQARLQELISPETANLYLAYALLDSQELPKEYISSIPWDGTLVLLQLQNDIRARHLN
                     SQTTETIQALLSSICSYSANPLPNRFLSDHFFIEYASFGGDLGLNDYVNSLEASWMKQ
                     VEQFEWAAPPVFQPNPAPGNRYPVRVTSLNPGLYGFVSTSGDNAGLVGDNQDTSWNEG
                     DAYASCMVLNQDYTDFPGSPLTALDATAAHEFNHAIQYGLGALHGHNIPDPVFIEGGA
                     TWMEDETFDEANDNYNYLWPFFSSCMSNYNRSPYPYWITFRGMLEPFGTGLPEGGEEV
                     MQLFWELTSQNLDGNLGALNRAFERQGTTLADVFHNYAIAVKFNRPCQAGYKRPYCLE
                     EGPEYIAAAGSTPVHGAISSVGSNFSGSVQDNFAINWVRLPENQGTYSVTLRNTSSGG
                     ALRSSVVCDMGSTLRVQAMDEIASPNTEVSGADFNSNGCDSVVVVVTNQAMTAENPSI
                     CTARSFQLLTSSRITPTPTPLLIYYFPFMPN"
BASE COUNT          943 a          912 c          939 g         1024 t
ORIGIN      
        1 ggtagacctg aagagctttt tctggatcct tccgggcgcg gtttgctaat gtttgagcct
       61 cgaccggtct caacaacccg atctgctcgc tccactcaag taatccggta tactgcaaca
      121 ggcggtcact tggctgatcg ctgccccgcg agctgagtgt atttgcaaaa tccaaaccca
      181 gcaggttggg tgtgagcgga ttatttcccg aaaagtgttc aatcattatc atcgattgtc
      241 cctgctgaat aaccgtctat aaccacttga caggttagat attttacgcg ataatctaac
      301 tagctataca attatagcag gttagtttgt agctgtcaaa tcgagccgaa ttaggagacc
      361 tggatgattt ggaggaaacc atggacaatg agttacaggt attgtcagaa gcccgctatc
      421 gctatgagaa ttatcttaag gaagcagatc acgagcgact cgcccatgaa gctcgcctta
      481 attatctgac acatcccaat cgcccattct caaacacgat accgacgcgg ctattggtct
      541 ggttgggtaa acatatgaca gtctggggag cttttcttgt ggaacgttat gatcccggca
      601 gggttaattc aaacaggcag ttgaatcata atatccaaac tacgcatggc tgctgattgt
      661 tttgcgagta tagcatggtg aagtcatggg taaaaattca acctgcacaa atcgttaata
      721 aaggtaaaat ttaactgtac atcgcacact gtaatctggt gaaataatcc agacagcaga
      781 cattctgaag gagtatgtgt atggccttac aggttggaga tatcgcccca gacttttgtc
      841 tggacagtca tcttgggcat ccggtcaggt taagtgatta tcgcggtaaa cggaatgttg
      901 tcctggcttt ctttccactt gcctggaccc cgatatgatc gggtcagatc ccatcctacc
      961 aggtagctct tgaccggttt gatggggtcc ggacccaggt gctgggtcta agtgtagata
     1021 gtgtgcctag tttgatagcc tgggcggaaa gcctgacagg tataacctat ccgctgttga
     1081 gcgatttcta tccccatggc aaggtcgccg agacttacgg tgtgctgcgc tctgaaggaa
     1141 aaagcgagcg cgccctcttt atcatcgata agcagggtgt gattcgatat gtagatgttc
     1201 atgatattga tcaccaacca gagaacgagg tattgtttgc cgaattggag aagatcgaac
     1261 catattatgc tgcccaatac gccgcccgga ttgcagctca gcaaaaaccc gaaccgcaga
     1321 tgatctctga ttcaaaagct gatgtgatcc tgtattgcac tccctggtgc ccaggctgcc
     1381 ggcgggcgcg ggcatttttg agggagaacc agatatcttt tgaggagatc aatattatgc
     1441 aagatcgagc ggccgccgcc caggtgcgtg aatgggctgg tgggtatgag acgaccccga
     1501 ctctaaacgt tcgtggtacg attgtgattg aatttgacct gaaccgggtc gcaaaactgt
     1561 taggaataaa gtgaatcagg aaaaaagtac tctctcttca aaaaatactg gcgccaggcg
     1621 cattcaacgc ctggctgccg gtttattgat tcttgttctg ttcgctgctg cctacctcaa
     1681 tagtttttac tcccctattt cagcgctggc ggatgctggt ggctggccca gcccaacttt
     1741 tgtgattatt cctaccatta attttcccac cgcgacttta ttccccacgg tagatctatc
     1801 caatcttaac cagcttctga ttacagaacc aacacctacc gcgaccctgt ttattgtccc
     1861 tacccctgga tcacctgaaa gcagaagtgc acttagttat ctatgcttac cgctgggggt
     1921 tggcatcctt ttaatcttaa tcctggtctc attgatggtg atgaggcgca ggcaagaata
     1981 attcgaagca acttagtggg gttactaaat gaggtgggat ttgaatgggc gcctgacagt
     2041 cgtggcagcc ctctttttct tcggtgtggg ttccttattc tttcagcctt ggaatacact
     2101 gctcggtctg gccagtgatc gggcagaaca gcctctctcc acacctgaac ggatcgagca
     2161 ggcgcgtctg caggagttaa tcagcccgga gacagccaac ctttacctgg cttacgcgct
     2221 tcttgactcg caagaattgc ccaaagaata tataagctca atcccctggg atggtacact
     2281 cgtccttctc caattacaaa acgatattcg tgccagacac ttgaattcgc aaacgactga
     2341 aacaatccag gcattattga gcagtatttg ttcgtattcc gcgaatccat tacccaatcg
     2401 ttttctgagc gaccatttct tcattgagta tgcctctttc ggcggtgatc tggggttgaa
     2461 cgattatgtt aattccctgg aggcatcctg gatgaaacaa gtggaacaat tcgaatgggc
     2521 ggcgcctcct gtgtttcaac ccaatccggc tccaggtaac cgatacccgg tccgtgttac
     2581 atcgttgaac cccggtctat atggatttgt ttcaacctct ggcgataatg ctggtctcgt
     2641 aggagataat caggatactt cctggaatga gggcgacgca tatgcgtctt gcatggtgct
     2701 caaccaggat tatactgact tccctggatc acccctgact gccctggatg ctaccgcggc
     2761 gcacgaattt aaccatgcga ttcaatatgg cctgggtgcg cttcatggtc acaatatacc
     2821 cgatcctgta tttatcgaag gcggcgcaac atggatggaa gacgagacct tcgacgaggc
     2881 gaatgataat tacaactatc tttggccatt ttttagcagc tgtatgagta attacaatcg
     2941 atctccctat ccatactgga ttactttccg gggtatgctg gagccctttg ggacaggtct
     3001 gcccgaaggg ggtgaggagg tgatgcaatt attttgggag ctgaccagcc agaatcttga
     3061 tggcaacctg ggtgccttaa atcgggcatt cgaacgccag ggtaccacat tagcagatgt
     3121 attccacaat tacgcgattg cggttaaatt taatcgtcct tgccaggcag gttataaacg
     3181 tccttattgt ctggaggaag gcccagaata catcgcagct gctggctcga ctcctgttca
     3241 cggggcaatt agcagcgtcg gaagcaattt ttcgggatct gtgcaggata acttcgcaat
     3301 aaactgggtc cggctgccag aaaaccaggg cacttatagt gtcacactgc gcaatacttc
     3361 aagtggaggg gctttgcgga gcagtgtggt gtgtgacatg gggagcacct tacgggttca
     3421 ggcgatggat gaaattgctt ccccaaacac cgaggtgagt ggggccgatt ttaactcgaa
     3481 cggctgtgac agtgtcgtag tagtggtcac caatcaggcg atgacagccg aaaatccctc
     3541 aatctgcacc gctcgttcgt ttcaattatt aacctcatct cggattacac caacccctac
     3601 cccgttattg atctattatt tcccctttat gcctaactga catccctata gtttcgggag
     3661 taccttcgtt agtcactctt taggctgtgg taatatagga ggttggagtc agtggcttct
     3721 cgactattaa gagaatactt gcccgggaaa atagttcccc ggtggaagaa tacgaaacaa
     3781 ggaaattaac aatggaatca ttccgtacac atattccc
//
LOCUS       DATMXK010000004        10019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_89910, whole genome shotgun sequence.
ACCESSION   DATMXK010000004 DATMXK010000000
VERSION     DATMXK010000004.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89910"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..728
                     /locus_tag="VN363_00080"
     CDS             <1..728
                     /locus_tag="VN363_00080"
                     /inference="COORDINATES: protein motif:HMM:NF019998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="TOBE domain-containing protein"
                     /protein_id="HWQ82927.1"
                     /translation="QQRVALARALAISPDLLLLDEPLSALDARVRANLRMEIAALQRR
                     LGITTIMVSHDQEEALTMADRIVVIDQGRVMQIGTPIEVYQTPATPFVANFVGLMNFL
                     PGEISREETWKVVCGDNALLVPEGENPAAPGAPVTVAIRPEDVRVLDGEKHPPNVLVA
                     QVEEVEFLGPYFRLHLELPSGTGQNGWLKLTTYIPPGQAHSTSLRKGARLAVYLPEHA
                     LRIFPRSGGRDDHPLLPDPGSRI"
     gene            688..2505
                     /locus_tag="VN363_00085"
     CDS             688..2505
                     /locus_tag="VN363_00085"
                     /inference="COORDINATES: protein motif:HMM:TIGR03262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0033225 - ATPase-coupled
                     2-aminoethylphosphonate transporter activity [Evidence
                     IEA];
                     GO_process: GO:0033223 - 2-aminoethylphosphonate transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative 2-aminoethylphosphonate ABC transporter
                     permease subunit"
                     /protein_id="HWQ82928.1"
                     /translation="MTTHSFPILGQESETLDGRLMRSHLPMPIPARAEVLKTTAARPT
                     ADWEGWLKRLLILFVVLWLFIGIVLPLFPLILRNLSDSQGRFVGLANYQKYFSSPALS
                     LAFTNSLTVSLLATLISVGLAFGYAYALTRTCMPAKRIFQSLAMLPLYAPSLAHGIGL
                     VYLFGRKGLVTTGLGWNIHLYGLPGIVLGEVLYCMPQALMILTIAASLADARLYEASR
                     TLRASPLRTFLTVTLPGLKYGLFSALFVCFTLAFTDFGVPKVVGGNFNVLATDIYKQV
                     IGQQNFAMGATISLLLLLPTGIAFVLDRIVQRRQAAILTARSVPFQPQPKRWIDLLAL
                     VYCLLVALAVAVVMSTVFLAAVIKLWPYQMSLTLRHFDFRTAGGGGYGAFFNSLRISF
                     YTALIGTTLTFGSAYLIEKLKEFRAARWLAYLVSMIPMALPGLVIGLAYIFFFNPLQW
                     KFAGLSIANPFAFLYNTMAVLVLANVVHFYTVSFMTATTALKQLDHEFEAVSASLAVP
                     FYRTLWKITLPLCLPAILEIAMYYFVNAMVTVSAVIFLYSPQLKLASVAIVNMDDAGD
                     TAAAAAMSALVILACLAVRLLYGWATRSLARHAQAWRTR"
     gene            2521..3666
                     /locus_tag="VN363_00090"
     CDS             2521..3666
                     /locus_tag="VN363_00090"
                     /EC_number="2.6.1.37"
                     /inference="COORDINATES: protein motif:HMM:NF010006.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047304 - 2-aminoethylphosphonate-pyruvate
                     transaminase activity [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-aminoethylphosphonate--pyruvate transaminase"
                     /protein_id="HWQ82929.1"
                     /translation="MKENHTLSSWKDKALFTPGPLTTSRTVKQAMLRDLGSRDTAFIE
                     IVRQMRQKLVALGGADESEYTAVLVQGSGTFGIEAVISSVIPPEGKLLVLINGAYGER
                     ILKIAGIHCIPTVPLRFVEDTVADPTSLDEALQADPSITHVAAVHCETTTGILNPIEL
                     YGEIVRQHRRTYFVDAMSSFGAYPIDLKALGIDFLVSSANKNLEGVPGFSFVLARREA
                     LAATKGFARSLSLDLLAQWEGLEDDGQFRFTPPTHAILAFQQALAELDEEGGIPARAL
                     RYRRNRELTVSAMQKMGFRTYLKPKDQGYIITSFYYPDHPNFDFQQFYARLSEKGHVI
                     YPGKLSHADCFRIGHIGRLEAGDVRALLAAIAETLVEMGVNLTKVEV"
     gene            3670..4692
                     /gene="phnX"
                     /locus_tag="VN363_00095"
     CDS             3670..4692
                     /gene="phnX"
                     /locus_tag="VN363_00095"
                     /EC_number="3.11.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR01422.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016827 - hydrolase activity, acting on
                     acid carbon-phosphorus bonds [Evidence IEA];
                     GO_process: GO:0009636 - response to toxic substance
                     [Evidence IEA];
                     GO_process: GO:0019635 - 2-aminoethylphosphonate catabolic
                     process [Evidence IEA];
                     GO_process: GO:0019636 - phosphonoacetate metabolic
                     process [Evidence IEA];
                     GO_process: GO:0019700 - organic phosphonate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphonoacetaldehyde hydrolase"
                     /protein_id="HWQ82930.1"
                     /translation="MDFVFRRSYRGPVKAVILDWAGTAVDYGSFAPAAVFLRLFESQG
                     VVITTEDARSGMGLMKKDHLRAILARPPVAKAWQAAHGTPASEADVDILFQNFIPMQA
                     AVLKDYAEPVCGVVDLVRELHDQEIKIGSTTGYTRAMMDVLAPEAARRGYAPDCIVCP
                     DEVPAGRPYPWMCYQNAIALQVYPMEALVKVGDTLVDVEEGLNAGMWTVGLALTGNLL
                     GLREEEVNALTDEGRLDKRERIEALLSHAGAHYVIDGIWELPPVLADIHARLARGERP
                     STCLTKRAVYRDYCGAMKLRLATEELVARLSPYADNVKTGSDDGSPSLYAMDRRLFGF
                     NTIIPE"
     gene            4706..5440
                     /gene="surE"
                     /locus_tag="VN363_00100"
     CDS             4706..5440
                     /gene="surE"
                     /locus_tag="VN363_00100"
                     /EC_number="3.1.3.5"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008253 - 5'-nucleotidase activity
                     [Evidence IEA];
                     GO_function: GO:0008254 - 3'-nucleotidase activity
                     [Evidence IEA];
                     GO_process: GO:0006950 - response to stress [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWQ82931.1"
                     /translation="MRILVTNDDGIFSPGLVALARAAIHFGQVRVVAPDGEQSAMGHA
                     ITIQRPLQYHRVHLDSLDAYRVNGTPADCVALGLYRWEGADLVLSGINLGSNLGHDVW
                     HSGTVGAAKQAVLLGIQAAAFSLALDEEEPEFEPLLPYVEQVIHTLLEDQRPKLVNVN
                     LPQNPRGIRWVRQSVRAYSGQVLEGEDPMGWRHYWFAASPQTDPEEDTDRWAVERGLI
                     AMTPLRLDLTDEEWLQRMRLIAEEEM"
     gene            5437..5796
                     /locus_tag="VN363_00105"
     CDS             5437..5796
                     /locus_tag="VN363_00105"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: protein motif:HMM:NF014225.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HWQ82932.1"
                     /translation="MKVIALIAHDGKKADMVAWATYNRDALARHQLVATEATARLLRE
                     KVGLEVEAVQPGPQGGDAQIGARVVEGQVDGVRGSRNAVASAVKRLLVAILDVSTELE
                     RIGDYAKGIARISLMIG"
     gene            6118..7434
                     /locus_tag="VN363_00110"
     CDS             6118..7434
                     /locus_tag="VN363_00110"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ82933.1"
                     /translation="MDSIKTEKGESLAVERMGLARDTLAMQVAIVTGGGRGIGREVAR
                     ALAWLEARVIIAELSDEGQETERLIREAGGEARFVRTDVSNPDDVAQLARLTQETFGP
                     ADILINNAILCPVAPVLEMDVDLWDQVLAVNLRGAFLTCQAFLPEMVARKRGVIVNMT
                     SLDAMPGLSAYIASKQALVGFSQSLAAEVGPRGARVIAFAPGMVDTPGIREVAERLPS
                     LLGMTREQFLSLPLHRAYQGLMPADHAGAATAFLVGALADVYHGETITGYEVLERSGF
                     LQTPAFGEMTPAPPHPGRELSPGLSHSQVVERALALCRKLQAVIEETGAGFNRLPVFV
                     RPMARSGFMKKAGGSLEDWARTLAGLATQLEGVAASDSAAEQALRAGLPGLQTSLVKL
                     RIYFQEVPEETARFSRDAKLLRQVQQASAEQAALIVDLLAALEAIQ"
     gene            7473..8174
                     /locus_tag="VN363_00115"
     CDS             7473..8174
                     /locus_tag="VN363_00115"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82934.1"
                     /translation="MIPRFPQDRNSPVSAPFMASKQEESMQTVGAEQRSSPNYRIEDL
                     GANLCITIPPPNPAAVGCLVGAIYLVSWAIGGLFLAGFVRGLNGTLVASTGFASLTGW
                     LLWLILFAGFTASLIFNLWGLLNREVVEITPGSISVARQFLGLGWRTQGLAEQIRALR
                     VVRAPWWSSLLGRSFWRRNWFSEWFQGTGWNPYGARRYALEMDSGDQIIRFGEGLSKA
                     EAKAILDLIQSRYAL"
     gene            8249..9049
                     /locus_tag="VN363_00120"
     CDS             8249..9049
                     /locus_tag="VN363_00120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561048.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82935.1"
                     /translation="MTSRKWFYPLVYFFLFLISALPPYTALPYDPRDTQGVIASILEV
                     SIRPYQAWGWVFHVGTLAVILLIVILPKNSGRVVSAYFGLNYLVVAALQTHAVTEKYG
                     FALQSGALVGFALLGALWLWVAWRGKLEASFRDVPAWRYLLLPLALLVFWAPLRVEGA
                     TFLPNFDPRLLLTSADYGLAYCFLTPVFLFLLILFYPRVDGFAFRVTAFNALLYGLFN
                     LALWRSPDTLWMGVLHLPLLVMSVTALWMSHFGRVGEPKSAAVVRLSS"
     gene            9102..9485
                     /locus_tag="VN363_00125"
     CDS             9102..9485
                     /locus_tag="VN363_00125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82936.1"
                     /translation="MAKLFRHENLLIRFLPVGILRGRNCDRASAQRQQGEHAAQLSWP
                     ACVFDVGGTAGILITGMIMLKGIFGMGTAYLGLLTVTGAIAIFLAASSFFSSSLSSIS
                     IILASFLTTVWVLLVGFGLYRLGRR"
     gene            9590..>10019
                     /locus_tag="VN363_00130"
     CDS             9590..>10019
                     /locus_tag="VN363_00130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82937.1"
                     /translation="MMAGSISTWIKRHALFAYFLLAYSISWAIQIPLAFQAQGLSQAS
                     IPFSTHYLSAYGPMLSAIIVTWLTGGIGGLKELMGRMLIWRVQPVYWLAAISPLALYV
                     IVALVLWLIQGNPLDLSVLGQVDFLPKMGWGVLPFWLLTSG"
BASE COUNT         1857 a         3004 c         3002 g         2156 t
ORIGIN      
        1 agcagcagcg ggtggcgctg gcgcgcgccc tggcgatttc acccgacctg ctcttgttgg
       61 acgagccgct ttccgctctc gacgctcggg tgcgggcaaa cctgcgcatg gagattgccg
      121 cgctacaacg ccgcctgggt atcacgacca tcatggtctc ccatgaccag gaggaagcgc
      181 tgaccatggc cgaccggatc gtggtcatcg accagggcag ggtgatgcaa atcggaacgc
      241 ccatagaggt ttaccagacg ccagccaccc ctttcgtggc caacttcgtc ggccttatga
      301 acttcttgcc cggggaaatc tccagggaag aaacctggaa agtagtttgc ggcgataatg
      361 ccctcctcgt gccggaaggc gaaaatccag ccgcccctgg ggcgccggtc acggtggcga
      421 ttcgcccgga ggatgtgcgg gttttggatg gcgaaaagca cccccctaac gtgctggtcg
      481 cccaggtaga agaggtggaa ttcctcggcc cgtatttccg ccttcacctg gagctccctt
      541 ccggtacggg acagaatggc tggctgaagc tgaccaccta cattcctccc ggccaggcgc
      601 attccaccag cctgaggaaa ggggcgcgcc tggccgtcta tctcccggaa cacgccctgc
      661 gcattttccc ccgcagcggc ggtcgagatg accacccact ccttcccgat cctgggtcaa
      721 gaatctgaga cactcgatgg ccggctgatg cgttctcact tgcccatgcc tattccagcc
      781 agggccgagg ttctcaagac cacagcggcg cgtccgacgg ccgattggga gggatggctg
      841 aagcgcctgt tgatcctgtt cgtcgtccta tggcttttca tcgggatcgt gctgccgctt
      901 ttcccactca tcttgcgcaa cctgagcgac tcacaaggcc ggttcgtcgg ccttgccaac
      961 tatcaaaagt atttctcaag ccctgcgctc tccctggcct tcaccaacag cctgaccgtc
     1021 tcgctgctgg ccacgctgat ctccgtcgga ttggcttttg gctacgccta cgcactgacc
     1081 cgcacctgca tgccggctaa gcgcatattt caaagcctag caatgctgcc cctgtacgcg
     1141 ccttccctgg cgcacgggat cggcctggtc tatctcttcg gccggaaagg cttggtgacg
     1201 accggcctgg ggtggaatat ccatttgtac ggtctgcctg ggattgtgct cggcgaagtg
     1261 ctgtactgta tgccccaggc gctgatgatc ctgaccatcg cggccagcct ggccgacgcc
     1321 cggctgtacg aagccagccg gaccctgcgc gcctcgccgc tgcgcacctt cctgaccgtg
     1381 acccttccag gactgaagta cggcctcttc agcgccctct tcgtctgctt caccctggcc
     1441 ttcaccgatt ttggcgtgcc caaggtggtg ggtggaaatt tcaacgtgct ggccaccgac
     1501 atctacaagc aggtgatcgg ccagcagaat ttcgccatgg gcgctaccat cagcctcctg
     1561 ctgcttctgc ctacggggat cgctttcgtc ctggaccgaa tcgtgcagcg gcggcaggca
     1621 gccattctga ccgctcgttc cgtccctttt cagccgcagc cgaagcgctg gatcgatctc
     1681 ctggcgctgg tttactgcct gctggttgcc ctggcggtcg ccgtcgtgat gagcacggtt
     1741 ttcctggccg cggtgatcaa actctggccg taccagatga gcctgaccct gcggcatttc
     1801 gacttccgca ccgctggagg aggcggatat ggcgcctttt tcaacagcct gaggatctcc
     1861 ttttataccg ctctcatcgg gaccacgctg accttcggaa gcgcctacct gatcgagaag
     1921 ttgaaagagt ttagagccgc ccgctggctg gcctacctgg tctcgatgat cccgatggcc
     1981 ttgccgggtc tggtgatcgg cctggcctac atcttctttt tcaacccgtt acagtggaag
     2041 ttcgccggcc tgtccattgc caacccgttt gcgttcctgt ataacaccat ggccgtcctg
     2101 gtgctggcca acgtcgtcca cttctacacg gtcagcttca tgaccgccac gacggcgctc
     2161 aagcaactgg atcacgagtt cgaggccgtc tcggcttcgc tggcagtgcc tttctaccgc
     2221 actttatgga aaatcaccct gccgctgtgc ctgcctgcca tcctggaaat tgccatgtat
     2281 tatttcgtca acgccatggt caccgtatcg gcggtgatct tcctgtattc gccccagctc
     2341 aagctggcat cggtggcgat cgtcaacatg gacgacgccg gggatacggc ggcggcagcg
     2401 gctatgtcgg cgctggtcat cctggcctgc ctggcggtgc gcctgttata cggctgggca
     2461 acccgcagcc tcgccagaca tgcccaagcc tggagaacac gctgaaaaag agtgatgcgc
     2521 atgaaagaaa atcacaccct ctcttcctgg aaagacaaag ccctgttcac gcccgggcct
     2581 ctgaccacca gccgcacggt caaacaggcc atgttacgcg acctgggttc gcgtgacacg
     2641 gcttttatcg aaatcgtccg ccagatgcga cagaagctgg tggcgctggg tggagcggac
     2701 gaaagcgagt acaccgccgt cctcgtgcaa ggcagcggca cgttcgggat cgaagcggtc
     2761 atctcctcgg tgatcccgcc ggaggggaaa ctgctggtgc tgatcaacgg cgcctatggc
     2821 gagcgcatcc tgaaaattgc cggcatccac tgcatcccca ccgttccatt gcgatttgtc
     2881 gaggatacgg tggcagaccc aacctctctg gatgaggcgt tgcaggctga cccgtccatc
     2941 acgcacgtgg ctgccgtcca ctgtgaaaca accaccggta tcctcaatcc catcgagttg
     3001 tacggggaaa tcgtccgcca gcaccggcgc acgtatttcg tggatgcgat gagcagtttt
     3061 ggggcctatc ctatcgacct gaaagccctg gggatcgact tcctggtttc gtcggcgaac
     3121 aaaaacctcg aaggcgtgcc cggcttctct tttgtgctgg cccgcagaga agcgctggcg
     3181 gcgacgaagg gctttgcccg cagcctgtct ctcgacctgc tggcgcaatg ggaagggctg
     3241 gaggacgacg ggcaattccg cttcacgccg cccacccacg ccatcctggc tttccagcag
     3301 gcgctggccg aactggatga ggagggcggc attcccgccc gcgccctgcg ctacaggcga
     3361 aatcgcgagc tgaccgtctc cgccatgcaa aaaatgggct tccggactta tctcaagccg
     3421 aaagaccagg gttatatcat cacctctttt tattaccccg atcaccccaa cttcgatttc
     3481 cagcaattct acgcgcgcct gagtgaaaaa ggccacgtga tctacccggg caagctcagc
     3541 cacgccgatt gtttccgcat tggtcacatc gggcggttgg aggcaggcga tgtgcgcgct
     3601 ttactcgccg ccatcgccga gaccctggtt gaaatgggcg tcaacctgac gaaagtggag
     3661 gtttgagcga tggattttgt gttccgccgt tcctatcgcg gcccggttaa agcggtcatc
     3721 ctggactggg cgggcacggc cgtagattat ggctcttttg ccccggccgc ggttttcctg
     3781 cgcttgttcg aaagccaggg cgtagtcatt actaccgagg atgcgcgctc tgggatgggc
     3841 ctgatgaaaa aagaccacct gcgcgccatt ctcgcccgcc cgccggtcgc caaagcctgg
     3901 caggctgcgc acggcacgcc cgcttccgag gcggacgttg atatcctgtt ccaaaacttc
     3961 atccccatgc aagcggcggt gttgaaggat tatgccgaac ctgtctgcgg tgtcgtggac
     4021 ctggtcaggg aattgcacga ccaggagatc aagatcggct ccaccaccgg ctacacccgt
     4081 gccatgatgg atgtcctggc tcctgaggcg gccaggcgcg gttacgcgcc cgactgcatc
     4141 gtgtgccccg acgaagtgcc tgctgggcga ccctacccct ggatgtgcta ccagaacgcc
     4201 atcgctttgc aggtctaccc aatggaagcc ctggtcaagg tgggcgatac cctggtcgat
     4261 gtcgaagaag gcctgaacgc cgggatgtgg acggttggcc tggcgctgac cggcaacctg
     4321 ctcggtctgc gagaagaaga ggtcaacgca ttgacggatg aaggacgcct ggataagcgc
     4381 gaaagaattg aagccttgct atctcacgcc ggagcgcact acgtgatcga cggaatctgg
     4441 gagctgccgc ctgtgctggc tgacattcac gctcgcctgg cgagagggga gcgaccatca
     4501 acatgtctga caaagcgtgc tgtatacagg gattattgcg gtgcaatgaa gttgagactg
     4561 gctactgagg aattggtggc gagactgtca ccatacgccg ataacgtgaa gacggggagc
     4621 gatgacggat ccccatcatt gtacgcaatg gatcgacgct tattcgggtt caatacgatc
     4681 attcctgagt aaaatgaggt tacacatgcg aatattagtg acgaacgacg acggcatttt
     4741 cagccctgga ttggtagctc tggccagagc tgcaatccac tttggccagg ttcgggtggt
     4801 ggcgcccgac ggcgagcagt ccgcgatggg gcacgccatt accattcaga ggcccttaca
     4861 ataccaccgg gtgcatctgg actccctgga tgcctaccgg gtgaacggca ccccggctga
     4921 ctgcgtcgcc ctgggactct atcgctggga gggggccgac ctggtgctct ctggcatcaa
     4981 cctggggagc aacctgggcc acgacgtctg gcattctggg acagtcgggg cagccaaaca
     5041 ggctgtgctc cttggtatac aagcagcggc gtttagttta gccctcgatg aagaggagcc
     5101 ggagttcgaa ccactgctgc cctacgtgga gcaggtgatc cacacgttgt tggaagacca
     5161 gcggcctaag ctggtcaacg tcaaccttcc ccaaaacccc aggggtatcc gctgggtccg
     5221 ccaatcggtg cgggcctaca gcggccaggt gctggaaggt gaagatccga tgggctggcg
     5281 gcattattgg tttgccgcct cccctcagac cgacccggag gaggacaccg accgctgggc
     5341 ggtggagcga ggtttgatag cgatgacacc actacgtttg gatctgacgg atgaagaatg
     5401 gctccagcgg atgcgcctga tcgctgagga ggagatgtga aagtaattgc tctgatcgct
     5461 cacgacggta agaaggccga tatggtcgcc tgggctacct acaaccggga cgctttggcc
     5521 cgccatcaac tggtcgccac ggaggcaact gccaggctgt tacgcgagaa ggtggggctg
     5581 gaggtggagg cggttcaacc tggcccgcaa ggcggcgacg ctcaaatcgg agccagggtg
     5641 gttgaaggcc aggtggatgg cgtccgaggt tcccgaaacg ccgttgcctc tgccgtaaag
     5701 cgcctgctgg tcgccatcct ggatgtgagc accgaactgg agcggatcgg cgattatgcc
     5761 aaagggatcg cccgcatcag cctgatgatc gggtaggaag agctgatcaa gccactgatc
     5821 gatatcccgc gcatggccga ccgggtaacc aatatctgcg agcgcaccat ctacgtggtc
     5881 accggtgaaa tgatagagat ggggttttcc gacgatgagt atctggcgcc agcggaataa
     5941 tcccgtttcc tgttgattgg ttgaaatcac gtttcccaca ccatcagaaa ggttacagga
     6001 gcgacctttt cacgccggcc gctcctgata atgggtatag gcgccggaaa cagaccttct
     6061 ccggcgccca tacttaaaat gtgataataa tggcaaagtg acatgaggac ccagatcatg
     6121 gattcgatca aaactgaaaa aggggaatcg ctggccgtgg aacgcatggg cttggcgcgc
     6181 gatacgctgg cgatgcaggt tgccattgtg acggggggcg ggcgcggtat cgggcgcgag
     6241 gtcgcccgcg ccctggcctg gctggaggcg cgtgtgatca ttgcggaact gtccgatgaa
     6301 ggccaggaaa cagagcgact gatccgggaa gcggggggcg aggcgcgttt cgtgcgcacc
     6361 gatgtctcta acccggacga cgtggcccaa ctggcgcgcc tcacgcagga gaccttcggg
     6421 ccggctgaca tcctgatcaa caatgccatc ctgtgcccgg tggcgccggt gctggagatg
     6481 gacgtggatc tatgggacca ggtgctagcc gtcaacctgc gcggcgcctt tctgacctgc
     6541 caggcattcc tgcccgagat ggtcgctcgc aagcgaggcg tcatcgtcaa catgacctcc
     6601 ctggatgcca tgccgggcct ctccgcctac atcgcctcca agcaggccct cgtgggcttc
     6661 agccaatccc tggcggccga ggtgggtccc cggggtgcgc gggtgatcgc ctttgctccg
     6721 ggaatggtgg acaccccggg aattcgcgag gtggccgagc gcctgccgtc actcctggga
     6781 atgacccgcg agcagttcct cagcctcccc ctgcaccggg cataccaggg gctgatgccg
     6841 gctgaccacg ccggggcggc cactgctttc ctggtggggg ccctggccga cgtgtaccac
     6901 ggcgagacga tcaccggcta cgaggtgctg gagcgcagcg ggttccttca gacgccggca
     6961 tttggcgaaa tgacgcccgc gccccctcat ccaggcaggg agctatcgcc gggtctctcg
     7021 cattctcagg ttgtagagcg tgccctggca ctctgccgta aactccaggc ggtgatcgag
     7081 gaaaccgggg ccgggttcaa ccgcctgccc gtcttcgttc gccccatggc gcgcagcggc
     7141 tttatgaaga aggccggggg aagcctggaa gattgggcgc gtaccctggc cggactggcg
     7201 acgcaacttg aaggcgtggc cgcctcggac tccgctgcag agcaagccct gcgcgcggga
     7261 ctgcccggtt tgcagacctc gctggtcaag ttgagaattt atttccagga ggtgcccgag
     7321 gagacggcgc gcttcagccg cgacgcaaag ctgctgcgcc aggtgcaaca ggcttctgcc
     7381 gaacaagcgg ccttgatcgt cgacctgctg gccgccctgg aagcgattca atgacctgcc
     7441 gccatggatg cttgcgctcg gtggagatga cgatgatccc ccgctttccc caggatcgga
     7501 atagccctgt ttcggcgcca ttcatggcga gcaagcagga ggaaagcatg cagaccgtcg
     7561 gtgcagaaca gcgctcttca cccaattacc ggatcgaaga cctcggggcg aatctgtgta
     7621 tcacgatccc gccccccaac ccggcagccg tgggttgcct cgttggggca atctatctgg
     7681 tatcctgggc gatcggagga ttgttcctgg ccggttttgt cagggggctc aatggcactc
     7741 ttgttgcatc caccggattc gccagtttaa caggatggct gttgtggttg atcttgtttg
     7801 ccggctttac agccagcctg atcttcaatc tttggggtct tttgaaccgg gaagttgtgg
     7861 aaatcacccc tggctcgatc tctgtggccc ggcaattcct gggcctgggc tggcgcacgc
     7921 aaggtctggc cgaacagatc cgggcactgc gcgtcgttcg ggctccctgg tggtcatctc
     7981 tgctgggacg gagcttctgg agaaggaact ggttttcaga gtggtttcag ggtactggct
     8041 ggaaccctta tggggcaagg cgatacgccc tggagatgga cagcggggat cagatcattc
     8101 gctttggtga agggttgagc aaagccgagg caaaggccat cctggatctg atccagagca
     8161 ggtatgcatt gtgaaggtcg gactccgcgg aaggtctgga accgggagcg gaaggcctca
     8221 tcttattctg gaaggaagaa aagagagcat gacatcacgc aaatggtttt accccctggt
     8281 ttatttcttt ctgtttctca tctcggcgtt acccccgtac accgccctcc cctacgaccc
     8341 gcgcgacacg caaggcgtga tcgccagcat cctggaggtc tccatcaggc cttaccaggc
     8401 ctggggatgg gtctttcacg tggggacgct cgcggtgatc ctcctgatcg tcatcttgcc
     8461 gaaaaactca gggcgtgtgg tatccgcgta tttcggtctc aactacctgg tggtcgccgc
     8521 gctccaaacc cacgccgtca cggaaaaata tgggtttgcc ctgcaaagcg gcgcgctggt
     8581 aggttttgcc ttgctgggcg ccctctggct atgggtggcg tggcgcggca agctggaagc
     8641 atctttccga gatgtcccag cctggcgcta cctgctcctg ccgctcgccc tgctggtgtt
     8701 ctgggcgccc ctgcgcgtgg aaggcgcgac gttcctgccc aacttcgacc cgcggctttt
     8761 gctcacctcg gcggattacg gcctggccta ctgctttttg acgccggtct ttttattcct
     8821 gctcatcctg ttctatcccc gggtggacgg ctttgccttt cgtgttacag ccttcaatgc
     8881 cctgctctac ggcctgttca acctggcctt gtggcgcagc ccggatacac tctggatggg
     8941 cgtgttgcac ctcccgctcc tggtgatgtc ggtgacggcc ctctggatgt ctcattttgg
     9001 gcgcgtcggt gaaccgaaat cagccgctgt ggttcgcctt tcgtcctgat cgacgccagg
     9061 cagattgtct cgatgatctt ttccgataat aaaactatgc tatggccaaa ctatttcgtc
     9121 acgagaacct actgatccgc ttcctgcccg tggggatcct gcgtggccgc aactgtgatc
     9181 gcgctagcgc gcagcgccag cagggagagc acgccgcgca actttcctgg ccggcctgtg
     9241 tattcgatgt tggaggcact gccgggattc tcataaccgg catgatcatg ctaaagggaa
     9301 tcttcggaat gggcacagcc tatttaggtt tgctgacagt gacaggcgct attgccattt
     9361 ttttagccgc aagctccttt ttttcaagct cgctaagctc tatctcgatt atcctggctt
     9421 cctttctcac taccgtgtgg gtcttgcttg ttggctttgg actctaccga cttggccggc
     9481 ggtgatccct tctgcaaacc ggctcatttt atcccaatgc gaaataacac gtgacttgac
     9541 caatgaccat tggaatgcgc ggcaaatatg atgatccaac aggagataca tgatggctgg
     9601 tagcatatcc acctggataa aacgacatgc gcttttcgcg tactttctcc tcgcctactc
     9661 gatctcatgg gcgatccaga tccctttggc attccaggcg caaggcttga gccaggcgtc
     9721 tattccgttt tccacccact acctatctgc ttatgggccg atgttatccg cgatcattgt
     9781 aacctggctc actggcggca tcggcggtct caaagagctc atggggcgaa tgctaatatg
     9841 gcgtgtgcag ccagtgtact ggctagcggc gatttccccg cttgcgctgt atgtgattgt
     9901 tgctcttgta ctctggctca ttcagggaaa tcccctggat ttaagcgtat tggggcaggt
     9961 tgactttctt cccaagatgg gctggggtgt cttgcccttt tggctattga cttctggga
//
LOCUS       DATMXK010000005         2689 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_10634, whole genome shotgun sequence.
ACCESSION   DATMXK010000005 DATMXK010000000
VERSION     DATMXK010000005.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2689)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2689)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2689
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10634"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            133..468
                     /locus_tag="VN363_00135"
     CDS             133..468
                     /locus_tag="VN363_00135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82938.1"
                     /translation="MKRLSLLVLVLALLAVSIPASAAGGRAGAIGEVYVTSQGLYYDT
                     FVTADVLPMHGRFQALVNGTTEFGPGDPGYLGGRWWEDTNGNGMQDEGDAFLLCPLLG
                     PGRTEPRGS"
     gene            689..>2689
                     /locus_tag="VN363_00140"
     CDS             689..>2689
                     /locus_tag="VN363_00140"
                     /inference="COORDINATES: protein motif:HMM:NF042814.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006304 - DNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Eco57I restriction-modification methylase
                     domain-containing protein"
                     /protein_id="HWQ82939.1"
                     /translation="MTAPGSILQLVSLFETHYKTYHSLNYPETQARLEFINPFFEALG
                     WDMLNKQGYAHAYKDVIHEDQIKVSGASKAPDYAFRIGGTRKFFVEAKKPAVNLKEEI
                     SPAYQLRRYAWSAKLPLSILTDFEELAVYDCREKPNLSDKPSRGRTLLFNYREYPERW
                     DEIANIFSREAVLKGSFDKYAESTKSKHGTAEVDDAFLGEIERWRELLAHNIALRNPG
                     VSQRDLNYAVQMTIDRLVFLRICEDRGIEFYGALQALLNGGEVYPRLVQLYHKADARY
                     NSGLFHFKDEKEQSDTPDTFTTGLDIDDKVLKGILESLYYPKSPYEFSVLPAEILGQV
                     YERFLGKVIRLTPAHHAVVEDKPEVKKAGGVFYTPTFIVEYIVRHTLGKLLEGRKPGE
                     VSVGRGAPLRVLDPACGSGSFLLGAYQYLLDWYLKGYIGEAEKWTKGKTPTLVQVQGG
                     EYHLTMAERKRILLDHIYGVDIDTQAVEVTKLSLLLKVLEGESEETLGRQLSLFQERA
                     LPNLGRNIKCGNSLIGPDFYAGQQSSFLEMEEQYRINAFDWHKEFTTVFMSSGGFDVV
                     IGNPPYIRIQALKEWAPKEVEFYKQRYQAASKGNYDIYVVFVEKALSLLNNYGQMGYI
                     LPHKFFNAKYGEPLRALIAGGRHLSKVVHFGDQQVFHNATTYT"
BASE COUNT          625 a          737 c          795 g          532 t
ORIGIN      
        1 acagtcgttt ttgtaagaaa tctgtaatcg ggggataagg gttggttcac tttttctgga
       61 tatattgggg gcataaccac tcaacaagga ttgagtgacc cttaacagga agctcgaaag
      121 gagaaattga cgatgaaacg gttaagctta ttggttctgg tgctggcgct gctggcagtc
      181 tcaatcccgg cctcagcggc tggcgggcgg gccggcgcaa tcggtgaggt gtatgtgacc
      241 agccaggggc tgtactacga caccttcgtg accgcggatg tgctgcccat gcacgggcgc
      301 ttccaggcgc tggtgaacgg caccaccgaa ttcggccccg gcgacccggg ttacctgggc
      361 gggcgctggt gggaagatac caacggcaac ggcatgcagg acgaagggga tgccttcctg
      421 ctctgcccgc tgctgggccc cgggcgcaca gagccgcgcg gctcgtaggc aggctgatta
      481 ttggacaaac agggacgccg ctgaggcgtc cctgtttgcg tgattcacgc catccagatt
      541 gctcaaaaat aataagagta acccggttgc accggcgtca ggctaaagga taaccgttgt
      601 acgccagtaa cattgaaaaa tagttgccct cttgcatatt cggcggtaag ataatcctgt
      661 cgcacccgcc aacaacccga gggaagccat gaccgcaccc ggcagcatcc tgcagctcgt
      721 ctcgcttttc gaaacccact acaagaccta tcactccctg aattaccccg aaactcaggc
      781 ccgcctggaa ttcatcaacc cgttctttga ggcccttggc tgggacatgc tcaataagca
      841 gggttacgcc catgcgtata aagacgtgat ccacgaagac cagattaagg tcagcggggc
      901 gagcaaagcc cccgactatg ccttccgcat cgggggaacg cgaaaattct tcgtggaggc
      961 gaaaaaaccg gctgtcaacc tgaaagaaga aatcagcccc gcctaccagc tgcgccgcta
     1021 cgcctggagc gccaagctgc ccctatccat cctgaccgat ttcgaagagc tggcggttta
     1081 cgactgccgt gaaaagccta acctgagtga caaaccctcc agaggccgca ccctgctgtt
     1141 caactacaga gaatacccgg agcgctggga cgaaattgct aatatcttca gccgcgaggc
     1201 tgtcctcaaa ggctctttcg acaaatacgc cgagagtacg aaaagcaagc acggcaccgc
     1261 cgaggtggac gatgctttcc tgggagagat cgagcgctgg cgtgagctgc tggcgcacaa
     1321 tatcgctctg cgcaacccgg gcgtcagcca gcgcgacctg aactacgccg tacagatgac
     1381 catcgaccgc ctggtgttcc tgcgtatctg tgaggaccgc ggcatcgagt tctatggagc
     1441 gctgcaagcc ctgctcaacg gcggcgaagt gtacccgcgc ctggtacagc tctaccataa
     1501 agccgacgcc cgctataact caggcctgtt ccatttcaag gatgagaaag agcagtccga
     1561 caccccggat acctttacca ccgggctgga tatcgacgac aaggtgctca aggggatcct
     1621 cgaaagcctg tactatccca aaagcccgta tgaattttcg gtgctgcccg ccgaaatcct
     1681 gggacaggtg tacgagcgct tcctgggcaa ggtgatccgc ctgaccccgg ctcaccacgc
     1741 cgtggtggaa gacaagccgg aggtgaagaa agccggcggg gtgttctata cccccacctt
     1801 tattgtcgag tacatcgtca ggcatacgtt gggtaagctg ctggaaggca ggaaaccggg
     1861 cgaggtgagc gtcggccgcg gcgcgccgct gcgcgtgctg gacccggcct gcggctccgg
     1921 ctcgttcctg ctgggggctt accagtacct gctggactgg tacctgaaag gctatatcgg
     1981 ggaagccgag aaatggacga agggcaagac gccaaccctg gtgcaggtac agggcgggga
     2041 ataccacctg acgatggcgg agcgcaagcg catcctttta gaccatattt acggggtgga
     2101 tatcgatacc caggcggtgg aagtgaccaa gctttccctg ctgctgaagg tgctggaggg
     2161 tgagagcgag gagacgctgg gcaggcagct cagcctgttc caggagcggg ctttgcccaa
     2221 cctgggccgc aatatcaagt gcggcaactc gctgatcggg ccggatttct acgccgggca
     2281 gcagagcagc tttctggaaa tggaggagca gtaccggatc aacgccttcg actggcacaa
     2341 ggaatttacg acggtgttta tgtcgtcggg tgggtttgat gtggtgatcg ggaatccgcc
     2401 gtatattcgc atccaggcgc tcaaagaatg ggcgccaaag gaagtggagt tctacaagca
     2461 gcgctatcag gccgccagca agggcaatta cgatatttat gtggtttttg tggagaaggc
     2521 tctaagcctg ttgaacaatt atgggcagat gggttacatc ctgccgcata agttcttcaa
     2581 tgccaaatat ggagagccat taagagcttt gattgctgga ggcagacatt tatcgaaagt
     2641 agtacatttt ggcgaccagc aagttttcca taatgccaca acctatact
//
LOCUS       DATMXK010000006         4683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_89812, whole genome shotgun sequence.
ACCESSION   DATMXK010000006 DATMXK010000000
VERSION     DATMXK010000006.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4683
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89812"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            143..1072
                     /locus_tag="VN363_00145"
     CDS             143..1072
                     /locus_tag="VN363_00145"
                     /EC_number="5.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RluA family pseudouridine synthase"
                     /protein_id="HWQ82940.1"
                     /translation="MENQIIELNFNGGEPTRLDKFLVQQLSDHSRSRLQALIKDGQVS
                     VDSVPAHKSGQMLDRPAQVLVHIPPPEPSRLQPEDIPLDIVFENEDLMVVNKPAGMVV
                     HPAAGHGQGTLVHAALAHAPEMEGIGGEQRPGVVHRLDKNTSGLILLAKNDRTHRMLQ
                     EQFRQRKVHKVYLALVDGQPPTPTGRIDAAIGRDTGERKLMAVVSDLKGRMATSEYRT
                     LAKYPAHTLLEVQPLTGRTHQIRLHLAFIGCPVAGDTIYGKRHSSIPLARHFLHAARL
                     TIQLPGEDSPRTFEAPLPVELQKTLESLTPVGQ"
     gene            1124..2197
                     /gene="ltaE"
                     /locus_tag="VN363_00150"
     CDS             1124..2197
                     /gene="ltaE"
                     /locus_tag="VN363_00150"
                     /EC_number="4.1.2.48"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559406.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="low-specificity L-threonine aldolase"
                     /protein_id="HWQ82941.1"
                     /translation="MDIVDLRSDTVTQPTPAMREAMARAVVGDDVYGEDPTVNRLQDL
                     AASLVGQEAGLFVPTGTMGNLAAILAHCQRGDEVILGNKAHTFMFEAGGISALGGVHS
                     SQLPNQADGTLELGAIEAAIRPDDAHHPITRLITLENTNNRCGGVALTPEYTRAVSDL
                     AHRHGLRLHLDGARIFNAAVSQGVRAIDLAGMTDSVTFCLSKGLSAPVGSVLCGSHAF
                     IQQAHRIRKQLGGGMRQAGVLAAAGIVALETMIDRLAEDHRRARMLADGLRQIPGLEL
                     DPAIPYTNMVFLNLADTAGVSRPERSGGISPAGHIAARLNDYGVRVGVAESRRFRLVT
                     HCWVTDAHVAQTIAAFQTVMAAG"
     gene            2460..3335
                     /locus_tag="VN363_00155"
     CDS             2460..3335
                     /locus_tag="VN363_00155"
                     /inference="COORDINATES: protein motif:HMM:NF012798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0015074 - DNA integration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine-type recombinase/integrase"
                     /protein_id="HWQ82942.1"
                     /translation="MRDPQPGVVYARNTLAAYQSDLNRFNRYIEKTAARSATLRDFTT
                     PQLQAFLHAEIDAGFHTHTIARRIASLRAFNLFLKQPIGDWEPVSSLALAGLHQRRGR
                     SLPTSHAAGLPASQPARLQPEHLSILWNAMEQAPQARARRDQALLAVCLETGLPLQRL
                     LALNLADLRDNCRKLSLEHPSGAKAQVILEWADQPLRRYLKEGRPELSHQPDELALWI
                     SQAGKRMSRQAVWQALRGWGLRADLPYPISPRLLSHTAAYRLESSGLSLQQIQLRLGH
                     QSSLSTLARLRRIAG"
     gene            3390..4226
                     /locus_tag="VN363_00160"
     CDS             3390..4226
                     /locus_tag="VN363_00160"
                     /EC_number="2.4.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004731 - purine-nucleoside phosphorylase
                     activity [Evidence IEA];
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="purine-nucleoside phosphorylase"
                     /protein_id="HWQ82943.1"
                     /translation="MKEVFTLAEIDRIAESIRPNLANAPRIGLILGTGLGSLAESVVE
                     QVRIPYRDIPDWPISTVLGHLGQLVSGTLQGQPVLVMQGRVHYYEGYSIAQVGLPTRV
                     LQRLGIEMLIVTNAAGAVNPSYEPGDLMLITDHLNLIGMAGLSPLRGPNLDELGPRFP
                     DMSQAYDRPLGVLAHQAARDAGFEMHAGVYCGLAGPSFETPADLRFLRGIGTDAVGMS
                     TVPEVTVARHGGTRVLGISGISNKANLDGNTLTTHEEVLEAGQQIVPKLIAVINGVLR
                     RI"
     gene            4293..>4683
                     /locus_tag="VN363_00165"
     CDS             4293..>4683
                     /locus_tag="VN363_00165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82944.1"
                     /translation="MAEALSFVRAFEAWIYLLLGLGGLFYIRKFILAWQELQDAGFGL
                     ERESAQGRVNLAAGMLVLILVLGVAEFMLVSFVAPTVPGANPLITPTVNLLATPTTTL
                     APTSTAGTPGGTTPSPTQTIAGTGACQV"
BASE COUNT          931 a         1424 c         1345 g          983 t
ORIGIN      
        1 ccattctggt gattggaatg tggattaaag agaaacaaca ggccagagat cgggttaccc
       61 gggctgaaaa tgactctggc cccggttccc aaccctctca ttctgaatcc caatcaacca
      121 gcctcccgcc tgaggattag ccatggaaaa ccagattatc gagcttaatt tcaacggcgg
      181 cgagcctacc cgcctggata agtttttagt ccagcagctc tccgaccatt ctcgctcgcg
      241 cttgcaggct ttgatcaagg atgggcaggt gtcggtggat agcgtgccag cgcataaatc
      301 cggccagatg ctggaccgcc ctgctcaggt gctggtccac attcctcccc cggaacccag
      361 ccgcttgcag cccgaagaca tccccctcga catcgttttt gagaacgaag acctcatggt
      421 ggtcaataag cccgccggaa tggtcgtcca tccagcagct gggcatggtc agggaacact
      481 ggttcatgcc gccctggcgc acgccccgga gatggaaggt attggcggcg agcagcgccc
      541 aggggtggtc catagactgg acaaaaatac ctcgggactg atcctgctcg ccaaaaatga
      601 ccgaactcac cgcatgctcc aggagcagtt ccgccagcgc aaggtccaca aagtttacct
      661 cgccctggtc gatggccagc ctcccactcc gaccggacgg atcgacgctg cgatcggtcg
      721 tgacactggt gagcgcaagt tgatggcggt ggtcagcgac ctgaaaggtc gaatggcgac
      781 cagcgagtac cgcactctgg ctaaataccc ggctcacacc ctcctggaag tccagccgct
      841 caccgggcgc acccaccaga tccgcttgca cctggccttt attggctgcc cggtcgcagg
      901 ggatacgatc tatggcaagc gccactccag tattcccctg gctcgtcact tcctgcatgc
      961 agcccgcctg accatccagc ttccgggtga ggattcaccg cgcaccttcg aagcgcccct
     1021 gccggtggag ctgcaaaaaa cgctcgaatc gcttaccccg gtgggtcaat gagctgttta
     1081 gtgtgccgcg ccgccggtaa actataaatc tacggagcaa cctatggata tcgtcgacct
     1141 gcgatccgat actgtcaccc agcccacccc ggcgatgcgt gaagccatgg cgcgcgctgt
     1201 ggttggcgat gatgtctatg gcgaagaccc taccgtcaac cgcttgcagg acctggctgc
     1261 cagtctggtc ggtcaggagg ctggcctgtt cgtccccacc ggaacaatgg gcaacctggc
     1321 tgccattctt gcccactgcc agcgcgggga tgaagtgatc ctcggaaata aagcccatac
     1381 gttcatgttc gaagccggcg gaatctccgc cctgggcggg gtgcattcca gccagcttcc
     1441 taaccaggcg gatggtaccc tggagttagg cgccatcgaa gccgcgatcc gtcctgacga
     1501 tgcccaccac ccgataaccc gcctgatcac cctggaaaac accaacaatc gctgcggggg
     1561 cgtggccttg accccggaat atacccgcgc ggtgagcgat ctcgcccacc ggcacggctt
     1621 gcgcctgcac ctggatggcg cccggatttt taatgccgct gtctcacagg gtgtgcgcgc
     1681 catagacctg gcaggaatga ccgattcggt tactttctgc ctgagcaagg gcctgtctgc
     1741 accggttggc tcggtattat gcggctcgca tgcctttatc caacaggctc accggatccg
     1801 caagcagctg gggggtggca tgcgccaggc aggtgtcctg gcagctgccg gaattgttgc
     1861 cctggaaacg atgatcgatc gcctggcaga agatcatcgc cgcgcccgga tgctggccga
     1921 tggcctgcgc cagattcccg gattggaact cgatccggca atcccataca ccaacatggt
     1981 tttcctcaac ctggctgaca ccgcgggggt atctcgacct gagcggagtg gaggtatctc
     2041 gcctgccggt cacatagccg cccggctgaa tgattatggc gtgcgcgtag gcgtcgccga
     2101 atcgcgccgc ttccgcctgg taacccactg ctgggtgact gatgcccatg tcgcccaaac
     2161 cattgcagct tttcagaccg taatggctgc cggttgagcc tgtctagcgg gttggcatgc
     2221 ttacagccga cctgccgggt gccagtagcc tgccgggtgc cggtagcctg ccgggtgcca
     2281 gtagcctgcc gggtgccagt agcctgttgg cagccatctc gtgattacgc gacagatccg
     2341 ggttggcggt gcatgttata attagaacac cataatctta ttgggcgggc gcgggatggt
     2401 ggaaaatcgg tagcagatct cttccaagtg ctgaatttgc acaagcgatt caggcctttg
     2461 tgcgtgatcc acaacccgga gttgtgtatg cacggaatac actggcagcc tatcagagcg
     2521 atttgaatcg ttttaaccgt tatatcgaaa aaactgccgc gcgctctgct acactgcgtg
     2581 atttcacaac accccagctc caggcatttc tgcatgcaga aatagatgct ggatttcata
     2641 cccacaccat cgctcgccgc atcgccagct tgcgagcgtt caaccttttt ttgaaacagc
     2701 ctatcggcga ttgggagcca gtttccagtc tggcactggc tgggctacat cagcgccgtg
     2761 gacgatcctt acccacaagc cacgcggctg ggctcccggc cagccagccg gcacgcctcc
     2821 agccggaaca tctcagcatc ctgtggaatg caatggaaca ggctcctcag gcgcgcgccc
     2881 gccgcgatca agcgctcctg gcggtatgcc tggagacagg tctacccttg cagcgattac
     2941 tggcattaaa cctggctgat ctgcgggata actgccgcaa gctttctctg gaacatcctt
     3001 caggggccaa agctcaggta atcctggagt gggctgacca accgctgcgc cgctatctga
     3061 aagaaggccg tccagaattg agccaccaac cggacgaatt ggctttatgg atcagccagg
     3121 caggtaagcg gatgtctcgt caggctgtat ggcaggcgct gcgcggctgg ggcctgcggg
     3181 cggatttacc ctatcccatc agccctcgcc tgctgagtca taccgcggcc taccggctgg
     3241 aatcctccgg cttatctttg cagcaaatcc agctgcgcct cgggcaccaa tcatccctat
     3301 ccacattggc tcgtttgcgc cggatcgcag ggtgaagtac gttctcagaa cagctcgtgc
     3361 tgcttgattg accgtctcag gagaattgga tgaaagaagt attcaccctc gcagaaattg
     3421 accggatcgc tgagtcgatt cgccccaacc tggccaatgc acctcgcatt gggctgatcc
     3481 ttggcaccgg cctgggttct ctggctgagt ctgtcgtaga gcaggtcagg attccctatc
     3541 gcgacatccc agattggccg atatccaccg ttctcggtca cctgggtcag ctggtcagtg
     3601 ggactttaca gggtcagcct gtcctggtca tgcagggtcg ggtgcattat tatgaaggat
     3661 actcgatagc tcaagtcggc ctgcccaccc gcgtgctgca gcgcctggga atcgagatgc
     3721 tcatcgtcac caatgcggct ggagcagtca accccagtta cgaaccgggc gatttgatgt
     3781 tgatcacgga tcatctgaac ctgatcggca tggcaggtct cagcccactg cgtggtccga
     3841 acctggatga gctgggaccg cgcttccccg atatgagcca ggcttatgac cgcccgttag
     3901 gcgttcttgc ccaccaggca gccagagatg ccggttttga aatgcacgcc ggagtatatt
     3961 gtggcctggc tggtccatcc ttcgagactc cggcagacct gcgtttcttg cgaggtatcg
     4021 gcacagatgc cgttggtatg tccactgtcc ccgaggtaac tgtggcgcgc cacggcggga
     4081 cacgtgtcct gggcatctca ggcatcagca acaaagccaa cctggatggg aacaccctga
     4141 ccacccatga agaagtactc gaagcaggtc agcagattgt tcccaaattg atcgccgtga
     4201 tcaacggcgt tttacgccga atttagtttg agacggaggg cgcctggtct tgtgtagtta
     4261 gtactgtggt ttagccccat ctccggagcg gcatggcaga agcactcagt tttgtgcgcg
     4321 ccttcgaggc gtggatatat ttgcttcttg ggctgggggg attattttat attcgtaaat
     4381 ttatcctggc ctggcaagag ctgcaagatg cggggtttgg cctggaacga gaaagcgccc
     4441 agggacgcgt gaacctggca gccgggatgc tggtgctgat tctggtgctg ggtgttgccg
     4501 aatttatgct ggtgagtttt gttgccccca cagtccccgg cgccaacccg ctcatcaccc
     4561 caacggtgaa cctgctggcg acccctacga caaccctggc gcccacttcg acggcgggca
     4621 ctccgggggg gaccactcct tcgcccaccc agacgatcgc agggaccggt gcctgccagg
     4681 tag
//
LOCUS       DATMXK010000007         3330 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_56813, whole genome shotgun sequence.
ACCESSION   DATMXK010000007 DATMXK010000000
VERSION     DATMXK010000007.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3330)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3330)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3330
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56813"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..930
                     /locus_tag="VN363_00170"
     CDS             <1..930
                     /locus_tag="VN363_00170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ82945.1"
                     /translation="NPQDKAMIQTEKLSKKFGEFSAVEGLDLEVHEGEVFGFLGPNGA
                     GKTTTVRMLTSLIAPTSGKAVVNGFQVGKDDQSIRRSVGILTETPGMYDNLSAQRNLE
                     IYAHLYEVKDIPGTVERYLRLFGLWDRRLDEAGSFSKGMRQKLAITRALLHEPRIIFL
                     DEPTAALDPEASHLVRDAILELKKEGRTIFLCTHNLDEADRLCDRIGVFKTRLLVMDT
                     PERLRSQLFGRKVVFHLKQADEKLAQTVVRLPYVREARCVDNRLLVTLDQPEEHNPEI
                     IRLLVGAGAEIQFVGELRHSLEDVYLQLVRNME"
     gene            942..1733
                     /locus_tag="VN363_00175"
     CDS             942..1733
                     /locus_tag="VN363_00175"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWQ82946.1"
                     /translation="MDKINTIIKKEWAEVFKNRMVLFSIIFMPLLFAVIPLVMLGVFN
                     QSSSASELTSEMPTQYMSTCPEGLSGGDCFQIYMVNQFTLLFMMLPLIIPINIAAYSI
                     VGEKTTHSLEPLLATPITTSELLIGKTLAAAIPAILATLSGFLIYAIGTLILVDNPLI
                     IRALLDPMWLLAVLLVGPLLAILSVNFALLVSSRVNDPRVAEQVSAVLIVPLLAVFFG
                     QISGLFLINNRLILLTALVLVAIDIAVVQMGVRLFQRETILTRWK"
     gene            1825..2607
                     /locus_tag="VN363_00180"
     CDS             1825..2607
                     /locus_tag="VN363_00180"
                     /inference="COORDINATES: protein motif:HMM:NF037015.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWQ82947.1"
                     /translation="MKIKRSKAILAAIICLLGAAVSQTARVTAQAETPLALSLRRNFG
                     FSSGSGDIQGNFTLRATPNQAVDKVVFYIDGELLGEDAAEPYELRFVTDNHPAGIHSL
                     TAKGFAPGGQELDSNIIKVNFRTPESARDSTFSILGYVFGALLLVMIISWAIPTVLGK
                     KATPLPPGAPRSYGIFGGAICPRCLRPFARSALGLNLVVGRLERCPHCGKWSLVHRAS
                     PEALRAAELAELDQLGVNPEVPTPSAEEHLRKELDDSRYQDL"
     gene            2647..>3330
                     /locus_tag="VN363_00185"
     CDS             2647..>3330
                     /locus_tag="VN363_00185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82948.1"
                     /translation="MKRWKSIPVLFCLLLLSSACLPSATPEVNPRGPGTPTLSDEAVL
                     GTKVAATLTAILPASSPVADTPQPGTPEATSPPAASPTSLQPTKAQPTLTTPVAMAAY
                     AREGVVYLWTEGTIRPLTSTGTEVFTVILSDDGRWVAYTRQVDDLRQEIWAVQTGGMD
                     GERLMSSADFAAIDPAALAVLPNQIGWVPGSHTLAFNTHQVFEGPGVSPYDDLHLIDV
                     DTFERRTPFL"
BASE COUNT          711 a          985 c          930 g          704 t
ORIGIN      
        1 aatccccagg ataaggcaat gatccaaaca gaaaaattaa gcaaaaaatt cggagagttc
       61 agcgcggttg agggcctgga tctggaggtc cacgaaggtg aagtattcgg cttcctgggc
      121 ccgaacggag cgggtaaaac cacaactgtg cggatgctca ccagcctgat tgcccccacc
      181 agtggtaagg ccgtggtcaa cggctttcag gtgggcaaag acgaccagag catccgccgg
      241 tcagtaggta tcctcactga gactcccggg atgtacgata acctgagcgc acagcgcaac
      301 ctggaaatct atgcgcatct ctacgaggtc aaagatatcc ccgggacggt agaacgctat
      361 ctgcgcctgt ttggcctgtg ggacaggcgc ctggacgaag ctggcagctt ttccaagggc
      421 atgcgccaga agctggccat tacgcgcgcc ctgctgcacg agccgcgcat catcttcctg
      481 gatgagccga ctgccgctct cgatccggaa gcctcccacc tggtgcggga tgcgatcctc
      541 gagctcaaga aagaaggccg gacgatcttc ctgtgcactc acaacctgga tgaagcggac
      601 cgcctgtgtg accggatcgg cgtatttaaa actcgcctgt tggtaatgga tacaccggag
      661 cggttacgtt cgcagctttt cgggaggaag gtcgtcttcc atctaaaaca agctgatgaa
      721 aagctggcgc agaccgtcgt ccgcctgcct tatgtgcgcg aggcgcgctg cgtcgataac
      781 cgactgctgg tgactctgga ccagccagaa gagcataatc ccgagattat acggctgtta
      841 gtcggcgctg gagcagaaat ccagttcgtc ggtgagctgc gccactccct ggaagatgtt
      901 tacttgcagc tggtacggaa tatggaatag gatagacagg catggataaa atcaatacca
      961 tcataaaaaa agaatgggca gaagttttta aaaacaggat ggtgctgttc agcatcatct
     1021 tcatgccctt gctcttcgca gtgatccccc tggtaatgct tggtgtattc aaccaatcga
     1081 gcagcgccag tgaactgacc agtgaaatgc ctactcaata tatgagcaca tgccccgaag
     1141 gtctgagcgg cggcgattgc ttccagatct acatggtcaa ccagtttacg ctcctgttca
     1201 tgatgctccc gctaatcatc ccgatcaaca tcgccgccta ttccatcgtc ggagaaaaaa
     1261 ctactcacag cctggagccg ctcctggcca caccgatcac aacttccgag ctgcttatcg
     1321 gcaaaaccct ggcggcagcg atccctgcca tcctggcgac actgagtggg tttttaatct
     1381 atgcgatcgg taccctcatc ctggtcgata acccgctgat catccgcgcc ctgctggacc
     1441 cgatgtggct gctggcggtg ctgctggtcg gtccactcct ggcaattctc tcggtgaact
     1501 ttgccctcct ggtctcttcg cgggtcaacg acccgcgcgt ggcagaacag gtctcggcgg
     1561 tgctgattgt gcccctgctg gcggtcttct tcgggcagat cagcgggctg ttcctgatca
     1621 acaaccgcct tatcttgctg acagctctgg ttctggtggc gatcgatatc gccgtggtgc
     1681 agatgggcgt acgccttttt cagcgtgaga ccatcctcac ccgctggaag taacccatga
     1741 tcgatagcgc agtattcaaa ccggcgtacg cctttttcag cgtgagacca ttctcacccg
     1801 ctggaagtaa aacgaggcag taaaatgaaa ataaaacgat ctaaagcaat ccttgcagcg
     1861 atcatctgcc tgctgggagc tgccgtttcc caaacggcca gggttacagc ccaggcagag
     1921 acaccgctag ccctgagctt gcggcgtaat ttcggttttt cgtcgggaag tggggatatc
     1981 caggggaatt tcaccctcag agccacccct aaccaggcgg ttgataaagt ggtcttttac
     2041 atcgatggtg agctgctggg ggaggatgcc gctgagccgt atgagctgcg ttttgtgacc
     2101 gataatcacc cggccggaat ccacagcctg actgcaaagg gatttgcgcc aggtggacag
     2161 gagctggatt ccaatatcat caaggtaaat ttccggaccc ccgaatctgc ccgtgactcc
     2221 acgttcagca ttttggggta cgtttttgga gctttgctcc tggtaatgat catctcctgg
     2281 gccatcccga cggtgttggg gaaaaaggcc acgcctctgc ctccgggtgc cccgcgcagc
     2341 tatggtattt tcggaggggc gatctgccca cgctgcctgc gcccattcgc ccgcagcgcg
     2401 ctcgggctta acctggtggt tgggcgcctg gagcgctgtc cgcactgcgg gaaatggagc
     2461 ctggttcacc gggccagccc tgaagcgctg cgcgccgccg agctggctga gctggatcag
     2521 ctgggagtga acccggaagt tcccaccccg agtgcagaag agcacttgcg caaggagctg
     2581 gacgattccc gctaccagga tttgtagcct tcgagcctgc cagactacaa gaaggagtaa
     2641 attcccatga agcgatggaa atctatcccg gtattgttct gcctcctgct gctcagcagc
     2701 gcctgcctgc cgtccgctac tccggaggtt aacccgcgcg gaccaggcac ccccaccctg
     2761 agcgacgagg ctgtgctggg aaccaaagtt gctgccaccc ttaccgccat tctgccagcc
     2821 agcagccctg tggcagatac accgcagcct ggcacaccgg aggcaacttc cccgccggct
     2881 gcatcgccta cttcattaca acccaccaaa gcacaaccta ccctgaccac cccggtcgcc
     2941 atggccgcct atgccaggga aggcgtggta tatctttgga ctgaggggac cattcgcccg
     3001 ctgacctcaa caggcacaga ggttttcact gtgatcctct cggatgatgg gcgctgggta
     3061 gcttacaccc gccaggtaga tgatctccgc caggagatct gggcggtgca gaccggcggg
     3121 atggatggag agcggctgat gagcagtgcc gatttcgccg ccatcgaccc cgcagccctg
     3181 gcagtcctcc ccaaccagat tggctgggtc cctggcagcc acacgctggc attcaacacc
     3241 caccaggttt tcgaaggtcc tggcgtttcc ccctatgacg atctgcacct gattgatgtc
     3301 gatactttcg agcggcgcac accgttcctg
//
LOCUS       DATMXK010000008         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_22460, whole genome shotgun sequence.
ACCESSION   DATMXK010000008 DATMXK010000000
VERSION     DATMXK010000008.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22460"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..698
                     /locus_tag="VN363_00190"
     CDS             <1..698
                     /locus_tag="VN363_00190"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF026183.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050661 - NADP binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ82949.1"
                     /translation="VEQVALGQRGILEGARTGLIHVDNSTIKPASARLISRVLGEKGV
                     ECLDAPVSGGDIGARDGTLTIMVGGPKAAVERVRPVLEAMGKKITYVGESGSGQIAKA
                     ANQIMVAAQMVAMSELLLFAKKAGADPRKVVEAIRGGAAQCWTLDVKPQRLFAGNRNP
                     GFKAYMQAKDLAIVLDTAREYGMPVPATAVHAQLYNAMLEMDMPDLDNSAVIGVLEIL
                     AGERLMDGLEAES"
     gene            717..1346
                     /locus_tag="VN363_00195"
     CDS             717..1346
                     /locus_tag="VN363_00195"
                     /inference="COORDINATES: protein motif:HMM:NF014627.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YitT family protein"
                     /protein_id="HWQ82950.1"
                     /translation="MANRQTFSQALRAYKVHWTLVDLRDFSFIAAGALLQALAMRLFL
                     VPMNLVSGGVSGIAQLINYYTDFPIGLMVFLGNIPLFLLGWRHLGGMRFAIRTAFAVI
                     AFSIFTDLLVFILPTNGLTSDLVLNTLYGGVASGIGYGLVYRGRGTSGGSDILARILS
                     PWRGISISQSYLLTDSLVILSKPSFVKRIPRRLWLLGRHMKFSEKDFYR"
     gene            1508..2101
                     /locus_tag="VN363_00200"
     CDS             1508..2101
                     /locus_tag="VN363_00200"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ82951.1"
                     /translation="MSANESLWLDQALEGDQQAFAQLVDLYQRPVFNLCYRMLGTAED
                     AEDASQETFLRAYRSLKRYDASRSFITWLLSIAAHYCIDQIRRRRYPVISIEDLPVPD
                     LPDHSPGMEAKLGNKQERERVRSLLQVLESTDRAIVVLYYWYDYSYEEIGQALSLTLS
                     AIKSRLHRARRAMAESWKENERKVEIPGRFAHESQTF"
     gene            2082..2621
                     /locus_tag="VN363_00205"
     CDS             2082..2621
                     /locus_tag="VN363_00205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82952.1"
                     /translation="MNHKPFEDWIFDPGSLADSNRADLHDHLEICEDCRLLSQAWEHV
                     SIELHNSPLAEAAPGFAKRWQLRVEADTSRQHRRQSLLLLTFCILGAGGLLAVLALLT
                     LPFTGSPVTLLMTWISRVWVLISTAGVIQDMLATLLRTLTSIVSPLWMILVLGIGSLI
                     AVLWAASLRILLHPRRVTL"
     gene            2618..3640
                     /locus_tag="VN363_00210"
     CDS             2618..3640
                     /locus_tag="VN363_00210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWQ82953.1"
                     /translation="MKNVYRLVLILLIVGIAFLLPATALAQGPNEDEVVVGDTYTLKS
                     GETLDGNLFVIGGNATIEAGATLNGDVVVGGGYLTISGLINGDTFATGGVIKLTDTAN
                     IQGDIVTLGGSVDRADGAIVSGEITEGFREPFKLFTPGGRFPAPPANIQVSGNPVVNA
                     VWSGFWVLMRSFLWAALAVLLVLFVPSATQHVSHAAVNKPWLTGGVGLLSSLVLPVVL
                     VILAITICLLPISLIGFLALGVAWAFGIVALGVETGKRLGIWLKQDWALPVLAGLGTF
                     ILTLVINGLNALLPCVGWLPSLLVGSVGLGAVILTRFGTQPYPPPDPISAQPAAPLLP
                     PVPPPE"
     gene            complement(3716..>4027)
                     /locus_tag="VN363_00215"
     CDS             complement(3716..>4027)
                     /locus_tag="VN363_00215"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558578.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWQ82954.1"
                     /translation="ADFIREASLILLRDEIPHGVAVRIDQYTEREETGLYVEATLFVE
                     RESHKAIVIGQAGSMIKRIGENARHEIENLTGRKVYLRLRVKVRKNWRDDEKVLRWFG
                     F"
BASE COUNT          870 a          994 c         1112 g         1051 t
ORIGIN      
        1 acgtcgaaca ggttgctctg ggccagcgcg gaatccttga aggtgcccga accgggttga
       61 tccatgtcga taactcgacg ataaaaccag cctcggcgcg cttgatcagc cgggtattgg
      121 gtgagaaagg tgttgaatgc ctggatgctc cggtgagtgg tggggacatt ggcgcaagag
      181 atggcacact gaccatcatg gtaggaggcc caaaagctgc tgtggagcgc gtccgcccgg
      241 tgttggaggc gatgggtaag aaaattactt acgttggaga gagcgggagt gggcaaattg
      301 ccaaagccgc caaccagatc atggtggctg ctcagatggt tgccatgtct gagctgctct
      361 tattcgcgaa aaaggctggg gctgatccgc gtaaggtagt ggaagctatt cggggtgggg
      421 ctgcccaatg ctggaccctg gatgtcaaac ctcagcgcct gtttgctgga aatcgaaatc
      481 ctggatttaa agcttacatg caagctaaag acctggcgat tgtcctggat actgcccgag
      541 agtatggcat gccggtgcca gccactgccg tgcacgccca gctctacaat gcgatgcttg
      601 aaatggatat gcctgatctg gataattcag cagtcatcgg agtgttggaa attctggctg
      661 gcgaacgatt aatggatggc ctggaagcag agtcttagcc aacaaccaga ttaatcatgg
      721 caaacagaca aacattcagc caggcgctcc gcgcatacaa ggtgcattgg accctggtag
      781 atttacgtga tttcagtttt attgcggctg gggccttgct gcaagccctg gctatgcgtt
      841 tattcctggt accaatgaac ctcgtttccg ggggagtgag tggcattgca cagctgatta
      901 attactatac cgatttcccg ataggtctga tggtgttttt aggcaatata cctctattct
      961 tgttgggctg gcgacatctc ggcggcatgc gctttgccat ccggactgca ttcgcggtaa
     1021 tcgcgttttc gatttttacc gacctgctgg tttttattct gccaaccaat ggtctcacct
     1081 cggatctggt gttgaatacg ctctatggtg gcgtagccag tgggatcggt tatggactgg
     1141 tgtaccgggg ccggggaacc agcggtggca gtgatatcct ggcgcgcatt ttgagccctt
     1201 ggcgaggaat atcaatctca cagagttacc tgctgaccga ttcgttagtc atcctttcaa
     1261 agccctcgtt cgtgaagcgg atccccaggc gtttatggtt attgggcagg cacatgaagt
     1321 tctcggagaa ggatttttac cggtagatca taaattgtga atccggtatt ttttgcacag
     1381 cagtgtccaa ctaaaaacta atcctgatcg tgcaaccata tatccattat tgcgtagatc
     1441 attccgaaac agatatgccc aaacataggc tcccacctgg gtttggcgaa tgaacaggag
     1501 caatattgtg agcgcaaatg agtcgctctg gcttgaccag gcgcttgaag gtgatcagca
     1561 ggcttttgct caattggttg atttatatca acgacctgta tttaacctgt gttaccggat
     1621 gctcggaaca gcagaggatg ctgaggatgc ttctcaagag acattcttgc gcgcctatcg
     1681 ttcgcttaag cgttacgatg ccagccggtc gtttatcacc tggttgttat cgatcgccgc
     1741 acactactgt attgatcaaa tacggcgccg gcgctatccg gttatttcaa tagaagacct
     1801 gcctgttccc gatttacccg atcactcgcc ggggatggaa gccaagctgg gcaacaaaca
     1861 ggaacgcgaa cgtgtgcgca gcttattgca ggttctggaa tctactgatc gcgccatcgt
     1921 agtcctgtat tactggtacg actattctta tgaggaaatt ggccaggctt tgtcgctaac
     1981 actatctgcg attaaaagca ggttgcaccg ggccagacgg gcaatggcag aaagctggaa
     2041 agaaaatgaa cgcaaagttg aaattccagg gaggtttgca catgaatcac aaaccttttg
     2101 aggattggat attcgatcct ggtagcttag ccgactcgaa tcgggcagat ctgcacgatc
     2161 accttgagat ttgtgaagat tgccgcctgc tctcccaggc ctgggagcat gtatcgatcg
     2221 agttacataa ttctccgtta gcagaagctg ccccgggatt tgcaaaacgc tggcagctgc
     2281 gtgtggaagc agacacttcc cgacagcacc gccggcagag tctgctactt ctgaccttct
     2341 gtattctggg ggcaggtggg ttgctggctg ttctggcttt gcttacgctc ccattcactg
     2401 gatcgccggt aacattgctc atgacctgga tctcgcgggt ctgggtgtta atttctaccg
     2461 ctggcgtaat ccaggatatg ctggcgactt tgctgagaac tttaaccagt attgtttcgc
     2521 ccctctggat gattctggtg ctcgggatag gcagcctgat cgcggtcctc tgggcggctt
     2581 cgctaagaat attattgcac ccgcggaggg tgaccctatg aaaaatgttt accggctggt
     2641 gctcatcctg ttgatcgtgg gtatcgcatt cttattacca gctacagcac tggcccaagg
     2701 accgaacgag gatgaggttg ttgtagggga cacctatacc ttaaaatccg gtgaaaccct
     2761 ggatggcaat ctgtttgtaa tcggtggaaa tgcaaccatc gaggcaggcg cgaccctgaa
     2821 cggggatgtt gtggtgggag gtggctacct gaccatcagt gggttgatta atggagacac
     2881 atttgccacc ggtggcgtaa tcaaactaac ggacacagct aatattcagg gcgatatcgt
     2941 tacattaggt ggaagtgtcg atcgcgccga tggtgcgatc gtctcaggtg aaatcaccga
     3001 gggttttcga gagcctttta aactgttcac accaggcggc agatttcctg cgccgcccgc
     3061 gaatatccag gtaagcggga acccggtggt taatgcagtc tggtccggct tctgggtgct
     3121 catgcgctca tttttatggg cagccctggc agtccttctg gtcttatttg tcccgtcggc
     3181 cacccagcat gtatctcacg cggctgtgaa taaaccgtgg cttacaggtg gtgtaggttt
     3241 attatccagc ctggtactac cggttgttct ggtgatcctg gcgattacca tatgtctgct
     3301 gcccatcagc ctgattggtt ttctggctct gggtgtggcc tgggcttttg gtatcgtcgc
     3361 tctgggtgtt gagactggga agcgcctggg gatctggctt aaacaagact gggcgctgcc
     3421 agttttagct ggtctcggta ctttcatcct gaccttagta atcaacgggc tcaacgcttt
     3481 actcccatgc gtggggtggt taccttcact gctggttggc tcggttgggc tgggtgcagt
     3541 cattttgacc cgttttggaa ctcagcctta tccacctccg gatccaatca gcgctcaacc
     3601 ggcagcaccg ctgctcccac cggtgcctcc cccggaatga tcttgccttt aaattaacag
     3661 cagggatgga taaccagtgc aattctgtcc atccctgctc ctatttccgg atcagttaga
     3721 atccaaacca gcgcaagacc ttctcgtcgt ctcgccaatt cttgcggacc ttgactcgca
     3781 gccggagata aacctttcgc ccggtaagat tttcgatttc gtgccgggca ttctctccga
     3841 tgcgcttgat catgctgccg gcctgtccaa tgacaatcgc cttgtgagac tcacgctcaa
     3901 cgaataatgt cgcttctaca tacaacccgg tttcttcccg ttctgtgtat tgatcgatgc
     3961 ggaccgcgac gccatgtgga atctcgtcgc gcagcaggat caggctggct tcgcgaataa
     4021 aatcggc
//
LOCUS       DATMXK010000009        10472 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_113428, whole genome shotgun sequence.
ACCESSION   DATMXK010000009 DATMXK010000000
VERSION     DATMXK010000009.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10472)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10472)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10472
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_113428"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..560)
                     /locus_tag="VN363_00220"
     CDS             complement(<1..560)
                     /locus_tag="VN363_00220"
                     /inference="COORDINATES: protein motif:HMM:NF013168.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000105 - histidine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HisA/HisF-related TIM barrel protein"
                     /protein_id="HWQ82955.1"
                     /translation="MRIIPVLDLLGGQVVHARRGERQNYQPVESVLCDSAEPRHVARA
                     FRERLGLNEHYIADLDAIQSTARNGGEIAVAHQGIISALAKDENFCILLDAGAGETSH
                     AQALLELGVSQVVIGAETLPRWEVLQELPTRLPPERLAFSLDMRGGKILSRCPEMAAL
                     EPLEALEQIQGAGWREVILLDLERVG"
     gene            559..1677
                     /locus_tag="VN363_00225"
     CDS             559..1677
                     /locus_tag="VN363_00225"
                     /inference="COORDINATES: protein motif:HMM:NF014686.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWQ82956.1"
                     /translation="MHSSPLVLVYEYFTGGGCPPGELPRSLAAEALGMLWALLVDFRR
                     WGAVRTLTALDARFAHNIPGLDRTTLPADFVVSIDPASQEQVFSTLLQRCDAVLIIAP
                     ETGGVLSRLTAQAEAAGVPVLGSSSASIAIAGDKSVCARLFSAAGLPAPLTFAAAFDT
                     AAQAADKIGYPVVIKPLDGVSSAGVCLVPAPADLPRALDILRQETIHKSILLQSYIQG
                     IHASVSLLVSAGRSLPLSLNRQRIEPGCPFEYLGGEIPLAHPAAVRAFELAQAAVALL
                     PGLQGYIGVDMLLSGPDAWLIEINPRLTTSYVGLRQVIPLNLAHAIWDACRHKVLPSS
                     VALDGRVEFSKDDYSSWGLEPARTLKSLSGTEAQEAHP"
     gene            1683..2738
                     /locus_tag="VN363_00230"
     CDS             1683..2738
                     /locus_tag="VN363_00230"
                     /inference="COORDINATES: protein motif:HMM:TIGR03123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydantoinase/oxoprolinase family protein"
                     /protein_id="HWQ82957.1"
                     /translation="MVGWDIGGANVKAALLCVEQGEIQSLQVSSLPFEIWRASDQLPR
                     VLQASLAAVAHGQPLSAHAVTMTAELADVFATRRAGVQFVFQSFQSAFPEAPAYALSL
                     AGQFEPLALAQTHPLDFAAANWVATAMWLARQFPDCLLVDVGSTTTDIIPILDGRLAS
                     SNRTDLERLSAGELVYTGLLRTNLAAIVQSLPVQGRLCRVASEYFAISADVHLILGHL
                     SPADYSCPTPDGRPPTLATARARLARLVCADTEQLSPDEIDALAGYIYTRQLCQIEDG
                     LSQVLTRYPRLGDAPVLALGSGAFLAAEAASRQGLKPHALPVIWEQSNSALAPGEVAP
                     CVAGALLLAASLEGNPE"
     gene            2735..3427
                     /locus_tag="VN363_00235"
     CDS             2735..3427
                     /locus_tag="VN363_00235"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82958.1"
                     /translation="MSFQAVLKVGGSLGRAGALPALCQEITRLGAAYPLLVVPGGGEF
                     ADQVRSVYHRFRLGDTAAHSMAVLAMDQYGYLLSELIPHSAPVASLRRAQRLAATGRI
                     PVLLPAALVRKVDPLPHSWEVTSDTIAAWIACQSASSRLILLKDVDGLYSSWRSPAAA
                     GELLLECSPAELDPHLGGVDESLARFLASAQLETWVINGSHPARLSQLLATGHTLGTC
                     IRPPTPFPGEAV"
     gene            complement(3399..4118)
                     /locus_tag="VN363_00240"
     CDS             complement(3399..4118)
                     /locus_tag="VN363_00240"
                     /inference="COORDINATES: protein motif:HMM:NF016367.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(5-formylfuran-3-yl)methyl phosphate synthase"
                     /protein_id="HWQ82959.1"
                     /translation="MRLMISVVSSEEAQEALDGGAEILDVKNPVEGSLGAQVPGVIRA
                     ITELAGGRVQVSAAIGDLPNLPGTAALAALGAAMCGADFVKVGLQGPRTSAEAVAVLG
                     AVKDALQGLPTVVIAGAYADFERAGTLDPKDLPGIAAAAGVGGCLIDTAIKDGKSLFD
                     FLKPEEVGELAVEAHAAGLLFAVAGTLGEKDLAIVQRLGADVVGLRTAVCQDNRRTGR
                     LDAEQVRRLVAAIKQPLQEKG"
     gene            4220..5233
                     /locus_tag="VN363_00245"
     CDS             4220..5233
                     /locus_tag="VN363_00245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82960.1"
                     /translation="MNLIESDYIETVEGLFFAVKGIAQPPDRVLAYLRYAPEIQGERH
                     RLGRAYRRLYHFAEQEQLLRSTYPRYLAFDPVTGLVLQTVPNTSIAHAYTSRQHLQDL
                     RQHPHRDPVEEAAFAFATNLQQISAIPWSSLGVSGSILIGLHTPASDLDMTIHGEQNC
                     WALHRALSTLLDQDSGEIRRLDETGMHALHAERSGDTQMSYADFIYSERKKVIQGRFR
                     DRTYFLRFLQEPAEYGESYGDRQYTPLGRATIEALIKDDRGAIFTPCNYKITGVRSID
                     GGTIPEISEIVSFRGRFCEQAQTGDTVRASGTVERVAHQSGRVWHRLILGGEFTDTMY
                     VRR"
     gene            5236..5790
                     /locus_tag="VN363_00250"
     CDS             5236..5790
                     /locus_tag="VN363_00250"
                     /inference="COORDINATES: protein motif:HMM:NF014494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavoprotein"
                     /protein_id="HWQ82961.1"
                     /translation="MKVKRIAWAITGAGHTLSDCANALLPYPNLDIFLSRAAEEVVRM
                     YGLAHLLEAPHLRVYKETRASSPLVVRLFSGAYHVLVIAPATSNTVAKCACGISDTLV
                     TNLFAQAGKSLVPVIVFPTDLAPEMDTTGPHGDAVKVYPRPIDLENTARLRTMPGVTV
                     VESVEEIARCLATYLSPENLPPSP"
     gene            5742..7070
                     /locus_tag="VN363_00255"
     CDS             5742..7070
                     /locus_tag="VN363_00255"
                     /inference="COORDINATES: protein motif:HMM:NF040121.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6513 domain-containing protein"
                     /protein_id="HWQ82962.1"
                     /translation="MPRYLFVTGKLAAQSLSDLLVRMAPEFEYEVAVLPISVAGLMDT
                     RFVFKKLPDARGCDRVMLPGLCHGDLQPLVEKLGVEVIRGPKSLKDIPAYFGRQGTLP
                     GYGEYHTRILAEIVDAYELSLDQIMARAEYYRASGADIIDLGCPVHGSFPAVDSVVAA
                     LKRAGFSVSLDTFNPDDALKASQAGLDYLLSVNSTNLELARRLSCKVVAIPDFEQGLE
                     SLERNLTQLESWGVPYIIDPILNPISFGFTESLCRYAEVRRRHPQAEIMMGLGNLTEL
                     TEADSTGIHAVMAGVLAELNIDYVLTTEVISWARGAVHELDIARKLMHYACQNQILPK
                     HLNSGLVTVKDPPYELYTEAELLAMHALVKDRNFRIFADRETVYIFNNQLFLKGLDPQ
                     ALFAQLDVQDPTHAFYLARELYRAVLAVRLGKSYTQETDLRWGYLSEVAS"
     gene            7067..8131
                     /locus_tag="VN363_00260"
     CDS             7067..8131
                     /locus_tag="VN363_00260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82963.1"
                     /translation="MNLPGFSPSRKPKDRDFVETLEGLLFCIVGYEHPPDKYIAYLKY
                     SPATSGPWQRSGTAYHRELAFYHAHQVAVTIAELEQQYPQYVHDSPVWDMRFSMVPVE
                     RAHTYYTPETRLAQIMAHPMDALEEATVHVVEEIQRAAAIPLSALGVTGSILLGIHSP
                     AFSDIDLTIYGRASARRLRDVMTGLALAGASPMDAAYVDEWCQGVVKHHALTYAQAYW
                     LISRRWNFVYFGSGRHTISLHPTRADAEITEVYGEHTYHDIGVARLTATLTGTQDAIF
                     LPAIYQIGSVAVLEGAPVEIVEICAYEGLFAQIGEIGQRIEARGKLERIDQGPLHRLV
                     VGSSHPSGSEYLLPVEILTP"
     gene            8128..8685
                     /locus_tag="VN363_00265"
     CDS             8128..8685
                     /locus_tag="VN363_00265"
                     /inference="COORDINATES: protein motif:HMM:NF016199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF447 domain-containing protein"
                     /protein_id="HWQ82964.1"
                     /translation="MIIESIFSTLGPGDQPNFAPMGITWGESVLTVRPFRATATYTNL
                     LATGCGVLSLTDDVLPFVQSSLYRVDLPHFPAHSIPGVILQGACSWRELQVLSVGGTP
                     ERAEIRCQVVHTGHQREFLGFCRASVAVLEAAILATRLQAHTPAAVQEIMRHYAQIVE
                     KTGGDNDRRAFHLVEQFIASSLDHA"
     gene            8678..9643
                     /locus_tag="VN363_00270"
     CDS             8678..9643
                     /locus_tag="VN363_00270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ribofuranosylaminobenzene 5'-phosphate
                     synthase family protein"
                     /protein_id="HWQ82965.1"
                     /translation="MLEILTPARLHLGLLDTNGNLGRLYGSIGVAIQQPNVILRAAPA
                     ASLQVHGLESDRVTAFARRFLAYHKLDLGARLELVAAIPGHVGLGSGTQLALALGAAL
                     AHLAGLDLSVAEIARSMGRGVHSGIGTNTFAQGGFVLDGGHSTLPAPPGAQKQAAVPP
                     VLVRQPFPEDWRFVVVIPEIDPGLSGEKETNAFQELPKAPPSRVARMCRLLVMQMLPA
                     LFEQNIVEFGHALTQIQRLVGASFAPIQGGQFADPLSDRLIAFLLDHGAAGAGQSSWG
                     PTVYGLVASQPAADLLAARASIFLATLRKGQVFTVAPDNHGARII"
     gene            complement(9721..10347)
                     /locus_tag="VN363_00275"
     CDS             complement(9721..10347)
                     /locus_tag="VN363_00275"
                     /inference="COORDINATES: protein motif:HMM:NF039786.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6448 family protein"
                     /protein_id="HWQ82966.1"
                     /translation="MFKSKFSKRTGLAIAGVLLAGVIFSALATPRPAQAHCDSEQGPV
                     ANAAHQALEKGDIKLILPYVQADAEKELTAAFKETQAVRKAGGKTMELADQFFIETAI
                     RLHRAGEGAAYNGVTDETTPEAILVADKAMASGSLDETYKMLDQIIQKGVQEKYEAVV
                     KAREEATHLGTVEAHRERVEAELMFEKYIYELSTLAAAAVPPAEGHNH"
BASE COUNT         1797 a         3459 c         2777 g         2439 t
ORIGIN      
        1 ctgcccaccc gctccaggtc cagcaggatc acttcccgcc agccagctcc ctggatctgc
       61 tccagtgctt ccagcggctc caacgccgcc atttccgggc agcgcgagag gatcttgcct
      121 ccccgcatat ccaggctgaa agccagtctt tccggcggca ggcgggtggg gagttcttgc
      181 agcacctccc atctgggcag ggtctccgca ccgataacca cctggcttac acccagctcc
      241 agcaaagcct gtgcgtggga agtctcgcct gctcccgcat ccaggaggat gcagaaattt
      301 tcatcctttg ccagcgccga aatgattccc tggtgtgcca cggctatttc gcctccattc
      361 cgggcggtgc tttggatcgc atccaggtca gcaatatagt gctcattgag tcccagccgc
      421 tcacgaaatg ccctggctac atggcgcggc tcggctgaat cgcataatac actctccaca
      481 ggctggtagt tctggcgttc gccgcgccgg gcatggacca cctgaccgcc tagcaggtcg
      541 aggacaggaa taattcgcat gcattcgtct ccgctggtat tggtctacga gtattttacc
      601 ggagggggct gcccgccggg cgagcttccc cgcagcctgg ctgccgaagc cctgggtatg
      661 ctttgggcgc ttctggtcga ttttcgccgt tggggcgcag tccgcaccct cactgccctg
      721 gatgctcgct tcgcacacaa catccctggg ctcgatcgta caaccctccc ggcggatttt
      781 gtcgtctcaa tcgatcccgc cagccaggaa caggtcttct ctacattgct ccagcgctgc
      841 gacgcggtcc tgatcattgc tccggaaacc ggtggcgtgc tctcccggct taccgcccag
      901 gcggaggctg ccggggttcc cgtattgggc tcttcctccg cctcaatcgc catcgccggc
      961 gataaatctg tctgcgcccg gctttttagc gccgccggtt tgcccgcccc ccttaccttc
     1021 gccgcggctt ttgataccgc cgcccaggct gcggataaga ttggctatcc cgtggttatc
     1081 aaacccctgg atggtgtcag cagtgccggg gtttgcctgg ttcccgctcc cgcagacctc
     1141 ccccgggcgc tggatatttt gcgccaggag actatccaca aatcgattct actccagtct
     1201 tatatccagg gtatccatgc cagcgtttcc ctcctggttt ccgccggtcg cagcctgcct
     1261 ctcagcttga accgccagcg gatcgagcca ggctgcccat ttgagtatct gggcggagaa
     1321 atccctctgg ctcacccggc agctgtccgc gcctttgagc ttgcccaggc tgcagttgcc
     1381 ctcctgccgg gtctgcaggg ttatatcggt gtggatatgc tcctgtccgg tccggatgcc
     1441 tggctgattg agatcaaccc tcgcctgacc acatcctacg tcggcttgcg ccaggtcatc
     1501 cccctgaatc tagcccacgc catttgggat gcctgccgcc ataaagtcct gccgtccagt
     1561 gtcgcgctgg acggtcgggt tgaattctcc aaggatgact actcgagttg ggggctggag
     1621 cctgccagaa ccttaaaatc cttatccggt actgaagctc aggaggctca cccttgatca
     1681 gtgtggttgg ctgggatatt ggcggcgcga atgtcaaagc ggcgctttta tgtgtcgaac
     1741 aaggcgaaat tcaatcactg caggtcagct cgctgccgtt tgagatctgg cgcgccagcg
     1801 atcagctccc ccgggttctg caggcaagtc tcgcggccgt ggctcacggt cagccgctct
     1861 ctgcccatgc tgtgaccatg actgccgagc tggcggatgt atttgccacc cggcgcgcag
     1921 gggtccagtt tgttttccag agcttccagt cagcctttcc tgaagctcct gcgtatgcgt
     1981 tgagccttgc cggtcagttc gaacccctgg cgcttgccca gactcacccc ctcgatttcg
     2041 ctgccgccaa ctgggtggcg accgccatgt ggctcgctcg ccagttcccg gattgtctgc
     2101 tggtcgatgt gggcagcacg accaccgata tcattcccat tctcgatggc aggctggcct
     2161 cttcaaatcg caccgacctg gagcgccttt cagccggtga gctggtttat acaggcttgc
     2221 tgcgcaccaa tctcgccgcg atcgtccaat ctctccctgt gcagggccgc ctttgccggg
     2281 ttgcttctga atatttcgcc atcagtgccg atgtccattt gatcctcggt cacctttcgc
     2341 ctgcggatta ttcctgccct accccggatg gccggcctcc cactctcgcc acagcccgcg
     2401 ctcgcctggc tcgcctggtg tgtgccgata ccgagcagct ttcaccggat gagattgatg
     2461 cgctggccgg atatatctat acccggcagt tgtgccagat cgaagatggt ctttcccagg
     2521 tcctcacccg ttacccccgc ctgggggatg ctcccgtgct ggcccttggc tccggagcct
     2581 tcctggctgc cgaagctgct tcccgccagg gtttaaaacc ccatgcccta ccggtcatct
     2641 gggagcagtc caattcggcg cttgcgcctg gcgaagtggc tccctgcgtg gcaggcgccc
     2701 tccttctggc tgcctcatta gaggggaatc ccgaatgagc ttccaggcgg tcctcaaagt
     2761 tggcggcagc cttggtcgtg ccggggcttt accggctctg tgccaggaga ttacccgtct
     2821 gggagccgct tacccgctgc tggttgtgcc tggcggcggt gaatttgctg atcaggtccg
     2881 ctcggtctat caccgcttcc ggctgggcga taccgccgcc cattctatgg ctgtgctggc
     2941 gatggaccag tacggttatc tgctcagcga gctgattccc cacagcgccc cggtcgccag
     3001 tctgcgtaga gcacagcgtc tcgctgcgac cgggcgaatt ccggtcctgc tcccggccgc
     3061 gctggtaaga aaagtcgatc ccctgccgca ctcctgggag gttacttccg atacgattgc
     3121 tgcctggatt gcctgccagt ctgccagctc gcggcttatt ttattgaaag atgtggatgg
     3181 attgtattct tcctggcggt caccggctgc tgccggtgag ctgctgctcg aatgcagtcc
     3241 ggcggaatta gaccctcatc tcggaggtgt ggacgagtct ttggctcgat ttttagcttc
     3301 tgcacaattg gaaacctggg tgatcaatgg ctcacatccg gcgcgcctgt cacagctgct
     3361 tgccaccggt cacaccctcg gcacctgcat ccgtccacct accccttttc ctggagaggc
     3421 tgtttgatcg ctgccaccag cctgcgcact tgctctgcgt ccagccgtcc tgtccggcgg
     3481 ttgtcctggc atactgctgt ccgcagtccc actacgtccg cccccagccg ctgcacgatc
     3541 gccaggtctt tctctcccaa tgtccctgct acagcaaaca gcaaacctgc cgcatgggct
     3601 tctactgcca gctcccccac ctcttctggt ttcaggaaat cgaatagcga cttgccgtct
     3661 ttgatggctg tatcgatcag gcagccgccg acccccgcag ccgctgcgat ccccggcagg
     3721 tccttcgggt ccagcgtgcc tgcccgctcg aaatcggcat atgccccggc gattaccacc
     3781 gtcggcaggc cctggagtgc gtccttcacc gcgcccagca cggccacagc ttcggcgctg
     3841 gttctgggtc cttgcaggcc aacttttaca aaatctgccc cgcacatagc tgcgcccagg
     3901 gctgccagtg ccgcagtgcc cggcaggttt ggtagatcgc cgattgccgc gctgacctgc
     3961 acccgcccgc cagccagctc tgtgatcgcc cggatcacgc ccggaacctg ggcgcccagc
     4021 gagccttcca ccggattctt gacgtccaga atttccgcgc cgccgtcgag cgcctcctgg
     4081 gcttcctcgc tgctcaccac gctgatcatc aatcgcatgc gtttttcctt gctcctctcc
     4141 tgcgtgagtc attgtagcat gccgattatg aaagttcaag cactggcctt tttcgaatga
     4201 taaaattaag ctacccgcga tgaacctgat cgaaagtgat tatatcgaaa ccgtcgaagg
     4261 cctttttttc gctgtgaaag gcatcgccca accgcccgac cgggtcctgg catacctgcg
     4321 ctatgcgccg gaaatccagg gcgagcggca caggctgggc cgtgcctatc gccgcctgta
     4381 tcactttgcc gagcaggagc agttactgcg ttctacgtat cccagatacc ttgctttcga
     4441 cccggttact ggcctcgtcc tgcagaccgt ccccaacact tcgattgccc acgcctacac
     4501 atcccgccaa cacctgcagg acctccgcca acacccccac cgcgatcccg tcgaagaggc
     4561 tgctttcgcc tttgccacca acctccagca aatttctgcc atcccctgga gcagcctggg
     4621 cgtctctggc tccatcctga tcggcttgca tacgcctgct tctgaccttg atatgaccat
     4681 ccacggcgag cagaattgct gggccctcca tcgcgccctg tccaccctcc tggatcagga
     4741 ttccggtgag attcgccgtc tggatgagac cggtatgcat gccctgcatg ccgagcgctc
     4801 gggcgatact cagatgtcct atgcagattt catctacagt gagcgcaaaa aggtgattca
     4861 agggcgtttt cgcgaccgca catattttct ccgcttcctg caggagccag ccgaatatgg
     4921 cgagagctat ggcgaccgcc agtacacgcc tctcgggcgc gccacgatcg aagcattgat
     4981 taaggatgat cgcggcgcca ttttcacccc ctgtaactac aaaatcaccg gcgtacgctc
     5041 tatagatggg ggaacaattc ctgagatttc tgaaatcgtc tctttccgag gtcgtttttg
     5101 tgagcaagcc cagaccggtg atactgtccg ggcttccggg acggttgagc gcgtcgccca
     5161 ccaatccggg cgggtgtggc atcgcctcat cctgggcggt gagttcacgg atacaatgta
     5221 cgtgcggagg tgagtatgaa agtgaaacgc attgcctggg cgatcaccgg tgcggggcat
     5281 actcttagcg actgcgccaa cgccttattg ccctacccta atctggacat cttcctctct
     5341 cgcgccgccg aggaggtcgt ccgcatgtat ggtctggcgc acctgctgga agcccctcac
     5401 ctgcgcgtgt acaaagagac ccgcgccagc tcccccctgg tggtccgtct gttctcaggg
     5461 gcttaccacg ttttggtcat tgctccagcg acctcgaaca ccgttgccaa gtgtgcctgt
     5521 ggtatctcgg acactctggt gaccaatctg ttcgcccagg ccggaaaatc cctggtaccg
     5581 gtgatcgtct ttcccaccga cctggctcct gaaatggata ctaccggccc gcatggtgat
     5641 gcagtcaagg tctatccccg gcccatcgac ctggagaaca ccgcccgcct gcggacgatg
     5701 ccgggtgtca ctgtggtgga gagtgtggag gaaattgccc gatgcctcgc tacttatttg
     5761 tcaccggaaa acttgccgcc cagtccctga gcgacctcct tgtccggatg gcgcccgagt
     5821 ttgagtacga ggtggcagtg ctgcctatct cggtggctgg cttgatggac acccgtttcg
     5881 tcttcaaaaa acttccggac gcgcgcggct gcgatcgagt catgctccct ggcctctgcc
     5941 acggagatct gcagcctctg gtcgagaaac tcggcgtcga agtgatccgg ggtcctaaga
     6001 gcctcaaaga catcccggct tactttggtc gccagggtac cctgcccgga tatggcgagt
     6061 accacacccg catcctggcg gagatcgtcg acgcctacga gttgagtctg gaccagatta
     6121 tggcccgcgc cgaatattat cgcgcttccg gggccgatat cattgacctg ggctgccctg
     6181 tgcacggcag ttttcccgcc gttgattcgg tcgtcgctgc tcttaagcgg gctggtttct
     6241 ccgtcagtct ggatactttc aaccccgatg acgccctcaa ggcctcccag gcagggttgg
     6301 attacctgct cagcgtcaat tccaccaatc tggagctggc tcgccgcctg tcctgcaagg
     6361 tggttgctat ccccgatttt gagcagggtc tggaatctct cgagcgcaac ctgacccagt
     6421 tggaaagctg gggtgttccc tatattatcg atccgatcct gaatccgatc agctttggct
     6481 tcaccgagtc cctctgccgt tacgccgaag ttcgccgccg ccatccccag gctgagatca
     6541 tgatggggtt gggcaacctc accgagctca ccgaggctga ttccaccggc atccatgccg
     6601 tcatggccgg ggtcctcgcc gaattaaaca tcgattatgt cctcaccacc gaggtgatca
     6661 gctgggcgcg cggcgctgtc cacgagctgg atattgctcg caaactgatg cactacgcct
     6721 gccagaacca gatcctgccc aagcacctca attccggatt ggttaccgtc aaagaccctc
     6781 cctacgagct gtataccgaa gccgagctgc tcgccatgca cgctctggtc aaagatcgca
     6841 atttccgcat ctttgccgac cgcgagacgg tctatatttt caacaaccag cttttcctca
     6901 aaggtctcga tccccaggcc ctttttgccc aactcgatgt ccaggaccct acccatgctt
     6961 tttatctcgc ccgtgagctt taccgtgcgg ttttggctgt tcgcctcggc aaaagctaca
     7021 cccaggaaac cgatctgcgt tggggctacc tgagcgaggt tgcttcatga acctgccagg
     7081 tttctcgcct tcccgcaagc ccaaagaccg tgatttcgta gagactctcg aaggtctgct
     7141 cttctgtatc gtcggttacg agcacccacc cgacaaatac attgcctatc tcaaatattc
     7201 cccggctaca tcgggtccct ggcagcgcag cggcactgcc taccaccgcg agctggcttt
     7261 ttaccacgcc catcaggtcg ccgtcaccat cgccgagctc gagcagcagt atccccagta
     7321 tgtccacgat tctcccgtct gggatatgcg tttttcgatg gtgcccgtcg agcgcgcgca
     7381 cacctattac acccccgaaa cccgcctcgc gcagatcatg gctcacccca tggatgcgct
     7441 tgaagaggcc acggttcacg tggttgagga aatccagcgc gccgctgcga tccctctatc
     7501 tgccctgggg gttaccggct ccatcctgct cggcatccac agcccggcat tttcagatat
     7561 cgatctcacc atctacggcc gcgccagcgc ccgccgcttg cgcgacgtca tgaccggtct
     7621 ggctctcgcc ggcgcttccc cgatggatgc cgcctatgtg gatgagtggt gccagggtgt
     7681 ggtcaaacat cacgctctca cctacgccca ggcctactgg ctcatctccc gccgttggaa
     7741 ttttgtctac ttcggttctg gtcgtcacac catctccctc catcctaccc gggccgacgc
     7801 tgagatcact gaagtctatg gtgagcatac ctaccacgat atcggcgttg cccgtcttac
     7861 cgccactctc accggtactc aggatgcgat cttcttgccc gccatctatc agatcggatc
     7921 ggtcgccgtc ctggaaggag caccggttga gatcgtggaa atttgcgcct acgaaggtct
     7981 cttcgcccag attggcgaga ttggtcagcg tattgaggcc cgcggcaagc tggaacgcat
     8041 cgatcagggt cctctccacc gcctggtggt tggcagcagc catcccagcg gctctgaata
     8101 cctgcttccg gttgagatcc tcaccccatg attatcgaat caattttctc caccctcgga
     8161 cccggagacc agccaaattt tgccccgatg ggtatcacct ggggagagtc cgtgctgacc
     8221 gtgcgcccct tccgcgccac ggcaacctac accaatctgc tggcgactgg ctgcggcgtc
     8281 ctcagcctga ccgatgacgt tctgcctttc gtccagagca gcctgtaccg ggttgatctg
     8341 ccccacttcc cggcccacag cattcccggt gtgattctcc agggagcttg ctcctggcgt
     8401 gagctgcagg ttctctcggt tggaggcaca cccgagcgcg ccgaaatccg ctgtcaggtc
     8461 gtccataccg ggcaccagcg cgaattcctg ggcttttgcc gggccagcgt cgccgtcctc
     8521 gaagccgcca ttctcgccac ccgcctgcaa gcccataccc ctgccgctgt tcaggagatc
     8581 atgcgccact atgcccagat cgtcgaaaaa accgggggag acaatgaccg ccgcgctttc
     8641 cacctcgtcg aacaattcat cgccagcagc ctcgaccatg cttgagatcc tcacccccgc
     8701 ccgcctgcat ctcggcttgc tcgataccaa cggcaatctt ggtcgcctgt atggcagtat
     8761 tggcgttgcc atccagcagc caaatgtgat cttacgcgcc gccccggccg catcgctcca
     8821 ggttcatggc cttgaatcgg atcgcgtgac agctttcgcc cgccgtttcc tggcttatca
     8881 caaactggac ctgggtgctc gccttgagct ggtcgcggcc atccccggtc atgtgggcct
     8941 gggctccggc acccagcttg cgcttgcttt aggtgctgca ctcgcccacc tcgccggtct
     9001 ggacctctca gtcgctgaga ttgcccgatc catgggccgt ggtgtccact cgggaattgg
     9061 cacgaatact ttcgcccagg gtggctttgt cctggatggt gggcattcga ccctccctgc
     9121 tcctcctggc gcccaaaagc aggctgctgt cccccctgtt ttggtccgcc agcccttccc
     9181 ggaagactgg cgttttgttg ttgttatccc tgaaattgac cccggtctga gcggcgaaaa
     9241 agagaccaac gcctttcaag agctgcccaa agctccgcca tctcgggttg ccaggatgtg
     9301 ccgcctgctc gttatgcaga tgcttcccgc cctctttgag caaaatatcg ttgagtttgg
     9361 tcacgccctc acccagatcc agcgtctggt tggcgccagc tttgctccaa ttcaaggcgg
     9421 ccagttcgcc gatcccctct cggaccggct gattgctttc cttctcgacc acggcgcagc
     9481 cggggctggc cagtcctcct ggggtcctac tgtatacggt ctggtcgcga gccagcctgc
     9541 tgcggattta cttgccgccc gcgcctccat tttcctcgct acgttgagaa aaggccaggt
     9601 ttttaccgtc gcccccgata accacggtgc ccggattatc tgactttttg cttcttccct
     9661 aaaaacaaaa agggtgctta cggtaattac cgcaaacacc ctcaccgttg ccaattaaat
     9721 ctagtggtta tgtccctccg caggaggtac agccgcggca gcaagggtcg agagctcgta
     9781 aatatacttc tcaaacatca gctcggcctc cacccgttcg cggtgggctt caactgtccc
     9841 caaatgtgtg gcttcttccc gcgctttcac tacagcttca tacttttcct ggacgccttt
     9901 ctggatgatc tggtccagca tcttgtaagt ctcatcgagc gatccagaag ccatcgcctt
     9961 atctgcgacc aggatagctt ctggtgtggt ctcatcggtt acgccattat atgctgcccc
    10021 ctcacctgcc cggtgcaagc gaattgctgt ctcaataaag aactggtcag ccagctccat
    10081 cgtcttgcct ccggctttgc gtaccgcctg cgtctctttg aaagcagcag tcagttcttt
    10141 ttctgcatcg gcttgcacat agggtaggat aagcttgata tcgccttttt ccaacgcctg
    10201 gtgggcagcg ttcgctactg gtccctgttc ggaatcgcag tgtgcctggg ccggtcgcgg
    10261 ggttgccagc gccgagaaga tcactcctgc caggagcacg cctgcaattg ccagacctgt
    10321 acgtttagaa aacttacttt tgaacatggt tcctccttgg tttgacctga actgattttg
    10381 gatcatgttc atatgataca ggcagtcata gagtatctcc atgatgtagg tcaattaact
    10441 gcaattcgta agctcatctg atcatactta ga
//
LOCUS       DATMXK010000010         2219 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_89653, whole genome shotgun sequence.
ACCESSION   DATMXK010000010 DATMXK010000000
VERSION     DATMXK010000010.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2219)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2219)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2219
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_89653"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1022
                     /locus_tag="VN363_00280"
     CDS             <1..1022
                     /locus_tag="VN363_00280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ82967.1"
                     /translation="VCSTRASASKRKSMRAGRFFTRKLNLLALFLLAIYAGIALAAPT
                     LAPPEEGSGDMPFQRLTEVKNFQPQPPGRTALMGTVATQARSYHLDVFYTFVWGARSA
                     FKFGLTAALGAALLGMLIGGFGAFISGWVQSLVLHITDAFLAFPLIVGVALFNQLFLI
                     SQRLSSVQTITINGVSQSTGTPPPLQVFFTQVNPVWLALVLFSWMPTARLVNTLVMTV
                     RQTDYVIAAHATGVGWLRILLRHILPNTIAPIVVLLTANIGGMVLIQATLTFIGMGGE
                     SAWGEILAQGRHWIIGSGGNPLMYWWVFVPITLALVVFGIAWNILGDGLNDWLNPRQT
                     EHAGT"
     gene            complement(1128..2201)
                     /locus_tag="VN363_00285"
     CDS             complement(1128..2201)
                     /locus_tag="VN363_00285"
                     /inference="COORDINATES: protein motif:HMM:NF021443.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5668 domain-containing protein"
                     /protein_id="HWQ82968.1"
                     /translation="MSNSSKSNPRREINLVWPMILIGIGLIFLLTNLGIVSGSVWEWV
                     GTLWPVFLILIGLESILNRRGLVGATVMICLGAIFLMANFGLLAMDIWSLVLQFWPIL
                     LIAIGFDIIFGRRSLILSLFGVLLVVAILIGAVWYSSFPVRSTQITATTINQTLEGAT
                     QADVQIDTGVGEVNIGSLDEGDALVAGSVPQGAGLDVRQEFSVDGEVANYRLWQTGSS
                     AWVFPAPRNGFRWDLQLNPEAIRNLELNLGVGQAELELQDIELENLQIQTAVGNTVLT
                     LPAQGNCQVQVEGAIGKLTVLVPAGVGLQIQASSGIGTTNVPEDYIRNGSNYRSPDFE
                     QAKNHVNLNVSNAIGLIEIKPLP"
BASE COUNT          527 a          573 c          554 g          565 t
ORIGIN      
        1 aggtctgctc gacccgcgca tccgcgagca aaaggaaatc catgcgagcc ggtagatttt
       61 tcacccgaaa gctaaatctg ctggccttgt ttcttctggc gatttatgct ggaattgccc
      121 tggcagcccc gaccctggca ccacccgaag agggcagcgg tgatatgcct ttccagagat
      181 tgaccgaagt gaaaaacttc cagcctcaac ccccaggccg cactgcgctt atgggaactg
      241 ttgccacaca ggcacgctct taccacctgg atgtgtttta cacctttgta tggggtgcgc
      301 gatcagcatt caaatttgga ttaaccgccg ctcttggagc ggcattgctg ggaatgctta
      361 tcggcggctt cggcgcattt atttctggct gggtgcaaag cctggtcttg cacattactg
      421 atgcgtttct ggcttttccg ctgatcgttg gggttgcgct ttttaatcaa ttgtttctta
      481 tttcccagcg gctttcttcg gtgcagacaa tcaccataaa tggagtctcg caaagtactg
      541 gaactccacc tccgttgcaa gttttcttta cccaggtcaa tccagtctgg ctggccctgg
      601 tgctcttctc ctggatgccg actgctcgtt tggtaaacac cctggtgatg acggtccggc
      661 agacagatta tgttattgct gcccacgcca ctggggtcgg ttggttacgg attcttttac
      721 gccacatctt gccgaatacg attgctccga tcgtagttct gctcactgcc aatattggtg
      781 ggatggtttt aatccaggcg accctcactt ttattggtat gggcggggaa tcagcctggg
      841 gagaaattct tgcccagggc cgtcactgga tcatcggcag cggcgggaat ccattaatgt
      901 attggtgggt gtttgttccg ataacattgg ctctggttgt ctttggaatc gcctggaata
      961 tactggggga tggcttgaac gattggttga atccaaggca gactgaacat gcaggcacat
     1021 aagaactgct gaaaatataa aaaaaacagc tggtctgcgg ttgctcacca gctgttttct
     1081 tcggtaaggg atgatattcg tggaatatgt ctgttttaga taaagtctca gggtaaaggt
     1141 ttaatctcga tcaaaccgat tgcgttcgag acgttcagat taacgtgatt tttcgcttgc
     1201 tcgaaatcag gcgagcgata gttagacccg ttgcgaatat agtcttcagg aacgttggtt
     1261 gttccgattc cggaagaggc ttgaatctgt aaaccgacgc ccgcgggcac aaggacggtc
     1321 agctttccaa tggcgccttc aacctgcacc tggcagttcc cctgagctgg tagcgttaaa
     1381 acagtattgc caactgcagt ttggatctga agattctcta attcgatgtc ctggagctca
     1441 agctctgcct gaccgacacc aagattgagt tcaagatttc gaatggcttc gggattcaac
     1501 tgtaaatccc agcgaaaccc attgcgtggg gcagggaata cccaggcgga tgagccagtt
     1561 tgccataatc gataattagc tacctcgcca tccactgaaa actcctggcg aacgtcaagc
     1621 ccagcgcctt gcggtacgct gcctgcgaca agcgcatctc cttcatccag gctaccgata
     1681 ttcacctcac ctaccccagt atcgatctga acatcggctt gagtcgcacc ttctaaggtc
     1741 tggtttatgg ttgtggcagt aatctgtgtg cttctgaccg gaaagcttga ataccacacc
     1801 gcgccaataa ggattgccac aacaagcagg acgccaaaaa gggacaggat gagcgatcgc
     1861 cgtccaaata taatatcaaa gccgatcgca atcagcagaa tcggccagaa ctggagcacc
     1921 aggctccaga tatccatcgc caacagtcca aaatttgcca tcagaaatat ggctcccagg
     1981 cagatcatca cggttgctcc gaccagccca cgccggttaa ggatgctctc aagaccgatc
     2041 aagatcaaga agacaggcca aagcgtaccg acccattccc agacgctccc actgacgata
     2101 cccagattgg tgagcaaaaa gatcaagccg atgccgatca ggatcatcgg ccataccagg
     2161 ttgatttccc ggcgtggatt tgatttagaa gaattactca ttttgatctc cctttgaac
//
LOCUS       DATMXK010000011         2919 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_22104, whole genome shotgun sequence.
ACCESSION   DATMXK010000011 DATMXK010000000
VERSION     DATMXK010000011.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2919)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2919)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2919
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22104"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            258..1433
                     /locus_tag="VN363_00290"
     CDS             258..1433
                     /locus_tag="VN363_00290"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWQ82969.1"
                     /translation="MATEKPSQPSKTCPTCGTRVSEDATRCMVCGTEFATGDRPSQPH
                     KAVQGSRMPELTLSLPVAIGVVALLIAVGALIVYFALQNTPTPVAEATVTPTITLTPT
                     STLTPTPETPTPSPSPQPSATPQTYVVKPGETCLGIALSFGVSINSIVLLNPELSADC
                     ANVYEAQRLLIPQPTPTSTPPPTSTLNAAEATEAACEKFPYTVQNTDTLSSIALNFNV
                     EMAAIREYNGMVNDIVRPGVVLDIPLCRRNATPGPSPTPTPPPPYSAPNLLLPVDGAS
                     FGIADELVTLQWAAVGTLRGNEAYAVTIEDITEGVGRREVAYVTDSKHIVSSEFRASD
                     SIPHVYRWSVITVRQTGSDDSGNPIWEPAGAVSAGRVFSWLSGGPPADTPAPPSATP"
     gene            complement(1529..2851)
                     /locus_tag="VN363_00295"
     CDS             complement(1529..2851)
                     /locus_tag="VN363_00295"
                     /EC_number="2.3.3.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559640.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="citrate (Si)-synthase"
                     /protein_id="HWQ82970.1"
                     /translation="MLLKEKIAAQLPAWQTRVKRLVKEFGEAKVGEVTIDQVYGGMRG
                     VKGLVSDISSVDPNEGIRLRGFTIPELIVKLPKPPGAEMPYVGGLYHLLLTGEVPTLE
                     EARAVEAEWNARSQVPGYVFDALNAMPEGTKAMTLFSLAILAMQKESVFTRAYETGMR
                     KDEYWQYTLEDSLNLTAKLPAIAAYIYYKLQGKDVRASAQPDLDWAGNFGKLLGVSDP
                     DFADLARLYFIIHSDHESGNVSAHATHLVGTTLSDVYYAASAGMNGLAGPLHGRANQD
                     ALHWLKQVRDQYGRVPSRQELETFSWEKLNAGQVIPGYGHAILRITDPRYTALYDFGR
                     KHFQQDDLFDLAVLVYDVVPDVLRRQGKAKDPWPNVDAISGTLLNHYGVEDCGTVCGF
                     YTVVFGVSRILGVSANLVWARALGQPLERPKSLTTADLEAIAAKAAVK"
BASE COUNT          639 a          904 c          804 g          572 t
ORIGIN      
        1 ggcgtcctga aaaatccagg cgtcctgaaa aatccaggcg tcctgaaaaa tccaggcgtc
       61 ctgaaaaatc cagggatctg acaatcgggc gacccaggcg gttctggtaa ggcggtcacc
      121 cggctgaaac gcaggcgaaa aggctggtat aatccccgag gcactccgcc caggttgtgg
      181 aattgaaaat acatccttag ggcaagccct tgcccggcaa gtggagaaac aaagcttgcc
      241 ccgggaatga agaggatatg gctaccgaaa aaccttctca accatctaaa acttgcccca
      301 cctgtgggac gcgggtcagt gaagacgcca cccgctgtat ggtgtgcggc accgaattcg
      361 ctaccggaga cagacccagc caaccgcaca aagccgtcca gggcagccgg atgcccgagc
      421 tgaccctcag cctgccagtt gcaattggag tggttgcgct gctgatcgcg gtcggagcgc
      481 tgatcgtgta tttcgccttg caaaacacgc ctactcccgt ggctgaggca accgtcacgc
      541 ccacgatcac cctgaccccg acttcgaccc tgacacctac gccagagacg cccacaccca
      601 gccccagccc acagcccagc gccacaccgc agacctacgt ggtcaagccg ggtgagacct
      661 gtctggggat tgccctgtca tttggcgtct caatcaattc gatcgtgctg ctcaatcctg
      721 agctttcggc agattgtgcg aatgtctacg aagcgcagcg cctgctgatc ccgcagccga
      781 cgcccacgag caccccgccg cccaccagca ccctcaacgc ggccgaagca accgaggcgg
      841 cctgtgaaaa gttcccttac accgtgcaga acaccgatac cctgagcagc atcgcattga
      901 atttcaacgt cgagatggca gccatccgtg agtacaacgg catggttaat gacatcgtcc
      961 gcccgggtgt ggtgttggat attccactct gccggcggaa tgccacccct ggaccttcgc
     1021 ctaccccgac acccccgccg ccctactcag cgccgaacct gctgctgcct gtggacggcg
     1081 catctttcgg cattgccgat gagctggtta ccctgcaatg ggccgccgtt ggcacgctgc
     1141 gcggcaacga agcatacgct gtaacgatcg aagacatcac cgaaggtgtg ggacgacggg
     1201 aagtagccta tgtcaccgac agtaagcata ttgtcagctc cgagttccga gccagcgaca
     1261 gcatcccgca tgtataccgc tggtcagtga tcaccgtccg ccagacaggc agcgatgaca
     1321 gcggcaatcc gatctgggag ccagccggtg cggtatctgc cgggcgggtg ttcagctggc
     1381 tcagcggcgg tccgcctgcc gatacaccgg cgccaccctc agcgacgccg taacatttta
     1441 atcagtctgt ccagacaaca ataaaaaccc caggggtttc ggaaaacccc tggggtttac
     1501 atatggcaga ttgggaataa gcaaagatct acttcaccgc ggctttggcg gcaatggctt
     1561 cgaggtcggc ggtcgtgagc gacttggggc gctcaagcgg ctgccccaga gcccgtgccc
     1621 agaccaggtt ggctgagacg cctaaaatgc ggctgacgcc aaatacaacc gtatagaagc
     1681 cgcagacagt gccgcagtct tctacgccgt aatggttcag caaagtgccg ctgatcgcat
     1741 ctacattcgg ccacggatcc ttggccttgc cctgcctgcg cagcacatcc gggacgacat
     1801 cgtacaccag cactgccagg tcgaataaat catcctgctg gaaatgttta cgtccgaaat
     1861 cgtagagtgc tgtatagcgc ggatcggtga tgcgcaagat ggcatgccca taaccgggga
     1921 tcacctgccc ggcgttgagc ttctcccagg agaaggtttc cagctcctgc cggctgggga
     1981 cccgaccata ttgatcgcgc acctgtttga gccagtgtaa ggcgtcctgg ttggcccgcc
     2041 cgtgcagcgg accggccagc ccgttcatgc cggctgaggc agcgtaatag acatccgaca
     2101 gtgtggtccc taccagatga gtggcgtggg cgctgacatt gccgctttcg tgatcggaat
     2161 ggatgatgaa atataaccgg gccaggtcgg caaaatcggg gtcgctgaca ccgagcagct
     2221 tcccaaagtt acccgcccaa tccaggtccg gttgggctga agcgcggacg tccttgccct
     2281 gcagcttgta atagatataa gcggcaatcg ccggcagttt ggcagttaga ttcaggctgt
     2341 cttccagggt atattgccag tattcatcct tccgcatccc ggtttcataa gcccgggtga
     2401 agacggattc tttttgcatc gccagaattg ccagcgaaaa gagggtcatg gctttcgtcc
     2461 cctcgggcat ggcattgagc gcatcgaaaa cgtagccggg cacctggctg cgggcgttcc
     2521 actcagcttc taccgccctg gcttcctcca aagtgggtac ttctccggtg agcagtaaat
     2581 gatataaccc gccgacgtag ggcatctctg ccccgggcgg cttgggcagc tttacgatca
     2641 gttcgggaat agtaaagccc cgcaggcgaa tgccttcgtt ggggtccacc gatgagatat
     2701 cgctcaccag tcctttgacc ccgcgcatcc caccatatac ctggtctatg gtcacctcac
     2761 cgaccttggc ctccccaaac tctttgacca gccgttttac ccgcgtctgc caggcaggca
     2821 gctgagcagc gattttttct ttgagcaaca tgatttcctc catgtttggc gattaaaaat
     2881 atctaaaaaa ggcgaagcgt ctcaacctca ccggtagac
//
LOCUS       DATMXK010000012         2018 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_34078, whole genome shotgun sequence.
ACCESSION   DATMXK010000012 DATMXK010000000
VERSION     DATMXK010000012.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2018)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2018)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2018
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34078"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(40..1317)
                     /locus_tag="VN363_00300"
     CDS             complement(40..1317)
                     /locus_tag="VN363_00300"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82971.1"
                     /translation="MLKIILQDLRSISPFNERARDLRVQNKPLWLLQRDVLAPYVEQE
                     LELPTGAALPETRSACLVYRDNLFFDEFYLAAFLAEAKKLNIAVQAAFTLTDPAFREH
                     ALPLSSSYSRAGEYYLADLWFYPHGPQETAEPLVIDLQSREVGYYHVPTYMAYEQGNM
                     TFNVPLRSLMAIDSWVHIYIADVVYGLFGRGARFEDRLARQPQFKLAILARAMYEGKQ
                     VLQSSQLVKIGKGCVIDPDAVIHGPTTIGNNVTINAGVVIENCIIGDNVNISQGCQLM
                     LSVVGDGAFLPFRAALFMTTFMDNSMVAQNTCLQMCVVGRNTFIGAGNTFTDFNLLSR
                     PLRAMDGEGSLQVANNLVLGGCVGHNCRIGSGLVIFPARTIESDVILFASPERRVIDR
                     DVSYEDSDHHRLANASAQPRLYPRPGETSSTAW"
     gene            complement(1336..>2018)
                     /locus_tag="VN363_00305"
     CDS             complement(1336..>2018)
                     /locus_tag="VN363_00305"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ82972.1"
                     /translation="YALDFWGFGESGTRRATYAVTDFISLVDQFMEELGIARAPLVGH
                     SMGGTVSLGAALQYPQRVQKVVVIGSPIDGASLALLLKMFGWKPFAALTYHNLWALKL
                     GFRQLARYYSRDPRWPDMMDRDISRVNLESFCQSIASLRRTDLRPNLHQVQVPVMGMY
                     GAKDIVVHPRQWRPLQESLPEARIERFSQAGHFIMLDDPQRFRSTLHEFLAAEPVSEL
                     AAQPAVTR"
BASE COUNT          480 a          581 c          566 g          391 t
ORIGIN      
        1 gctcgtacga acaatccaaa gagaggaatt gcgaggcgct taccaggcag tggagctggt
       61 ttctccgggg cgcgggtaca accggggctg cgcactggca tttgccaggc ggtgatggtc
      121 gctgtcttca tacgacacat cccgatcaat cacccgccgt tctggcgatg caaacaggat
      181 cacatccgat tcgatcgtac gagccgggaa gataaccaac cctgagccta tccggcagtt
      241 atgccccaca cagccgccca ggaccagatt attggcaact tgcaggctgc cttcgccatc
      301 catggcgcgc agcgggcggg agagcaaatt gaaatcggtg aacgtgttgc cagccccgat
      361 aaaagtgttc cgcccgacca cacacatttg caggcaagta ttttgggcga ccatactgtt
      421 atccatgaaa gtggtcatga ataaagctgc ccggaagggc aggaaggcgc catcgccaac
      481 aacggaaagc atcagctggc agccctgaga aatattgaca ttatcgccaa taatacagtt
      541 ttcgatcact accccggcgt ttatggtgac attgttgcca atcgtcgtcg gtccgtggat
      601 aactgcgtcc gggtcgatca cacagccctt gccgatcttc accagctgcg aggattgcaa
      661 aacctgcttg ccttcgtaca tagcccgcgc caggatcgcc agcttgaact gcggctgccg
      721 cgccagacga tcctcgaaac gcgctccccg cccaaacagg ccgtagacga catccgcgat
      781 gtaaatatgc acccacgaat cgatcgccat caggctgcgc agggggacat tgaaggtcat
      841 gttgccttgc tcgtacgcca tataggtggg cacatggtaa taaccgactt cccgggactg
      901 cagatcgatc accagcggct cggcggtttc ctgtggccca tgcgggtaaa accacagatc
      961 agccaggtaa tattcaccag cccgagaata cgagctgcta aggggcaggg cgtgctcgcg
     1021 gaaagccggg tcggtgagcg taaaagccgc ctgtacggcg atgtttagct ttttagcttc
     1081 ggccaggaag gcagccaggt aaaattcgtc aaagaacaga ttatcgcgat acaccaggca
     1141 ggcgctgcgg gtctccggca aggctgcgcc ggtgggtaat tctaattcct gttcgacata
     1201 tggcgccagg acatcgcgct gcagcagcca cagcggttta ttttgcacac gcagatcgcg
     1261 cgcccgctcg ttgaatggag aaatggaacg caggtcttga aggataatct ttagcataac
     1321 agcggtgaaa ataacttatc gggtgacggc aggttgagcg gcaagctcgc tgaccggttc
     1381 ggctgccaga aactcatgga gagtgctccg gaagcgttgg gggtcgtcca gcataataaa
     1441 atgcccggcc tgcgagaatc gttcgatgcg cgcttcgggc aagctttcct gaaggggccg
     1501 ccactggcgc gggtggacga cgatatcctt cgccccatac attcccatga ccgggacctg
     1561 cacctggtgc aggttggggc gcaggtcggt gcggcgcaag ctggcaatac tctgacaaaa
     1621 ggattccaga ttgacccgcg agatatcgcg atccatcatg tctggccagc gtgggtcgcg
     1681 cgaataatac ctggccagct gacgaaaccc gagcttcaac gcccataaat tatggtaagt
     1741 caaggctgca aatggcttcc agccaaacat cttcaataat aacgccagtg aagcgccatc
     1801 gatcggagaa ccaatcacga ccactttttg cacccgctgc gggtattgga gagcagctcc
     1861 caggctgacg gtcccaccca tgctgtgccc caccagcggc gcccgggcaa tccccagctc
     1921 ttccataaat tgatctacca ggctgataaa gtccgttacg gcgtaagtgg cgcgccgggt
     1981 gcccgattcg ccaaaccccc aaaaatccag cgcataag
//
LOCUS       DATMXK010000013         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_146970, whole genome shotgun sequence.
ACCESSION   DATMXK010000013 DATMXK010000000
VERSION     DATMXK010000013.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146970"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..558)
                     /locus_tag="VN363_00310"
     CDS             complement(<1..558)
                     /locus_tag="VN363_00310"
                     /inference="COORDINATES: protein motif:HMM:NF012780.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWQ82973.1"
                     /translation="MTSNLEIHGRTGTLVTDDTAHDITRVEELAYDIRIEEVMTTDVQ
                     ALSPDAPISDLVELLRTHRISGAPVVENGYLIGIVSLEDLIRALTANDTSSPVRKYMN
                     PGVITVNGFDPVIKAMERFAKDKVGRFPVIDVNGKLVGMITKGDVTRGLLVSLQRDYQ
                     AEEVRRYRASHLFEDINSDRTSLILR"
     gene            complement(564..824)
                     /locus_tag="VN363_00315"
     CDS             complement(564..824)
                     /locus_tag="VN363_00315"
                     /inference="COORDINATES: protein motif:HMM:NF018753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DRTGG domain-containing protein"
                     /protein_id="HWQ82974.1"
                     /translation="MSDVLATAQPESILLTGLNNPQTIRTAQMADIRAIIFVRGKSPT
                     PDVVEVAEREQIPLISSPHGMFELCGRLYTAGLPSLEEKFTD"
     gene            complement(892..1737)
                     /gene="fdhD"
                     /locus_tag="VN363_00320"
     CDS             complement(892..1737)
                     /gene="fdhD"
                     /locus_tag="VN363_00320"
                     /inference="COORDINATES: protein motif:HMM:TIGR00129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0009061 - anaerobic respiration [Evidence
                     IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate dehydrogenase accessory
                     sulfurtransferase FdhD"
                     /protein_id="HWQ82975.1"
                     /translation="MNEPLPTPNQTVQYQQYVSGIWKPLSSEVITEQPVSLTVNGEVW
                     LTLMCTPIDLEALATGFLYNEGFIQGTEDVADVRVCDNQENVDVWLTQAVEKPRQWRR
                     TTGCTGGVTSISTEYKGRPVVNGGIISPQKVCQLVARLFDVQDLYRKSGGVHTSALSD
                     GEQILITAEDIGRHNTLDKIAGKLLMASLQPQRRILLTTGRISSEMLQKAGRINASIL
                     ISRTSPSSLSIQMAERQGITLIGYARRNQFNVYTHPERMENILASEDRLDHTENETIG
                     DRKYR"
     gene            1966..>2393
                     /locus_tag="VN363_00325"
     CDS             1966..>2393
                     /locus_tag="VN363_00325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558940.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyphosphate kinase 2 family protein"
                     /protein_id="HWQ82976.1"
                     /translation="MDRYRVKPGQKIKLDKWDPNDRSLYDLDKDQGEEALDKLNDQLE
                     KLQELLYVEGKHKILIVLQGMDTSGKDGVIRHVFDGVNPQGVRVASFKVPTPEELGHD
                     YLWRIHKQTPQRGEIVIFNRSQYEDVLVVRVHGLVPEEVW"
BASE COUNT          552 a          599 c          680 g          562 t
ORIGIN      
        1 gcgtaagatc aggctggtgc gatccgagtt gatgtcctca aagaggtggc tggctcggta
       61 gcggcggact tcttcagcct gataatcgcg ctgcaaggaa accagcagcc cccgcgtaac
      121 gtcacctttg gtgatcatgc cgaccagctt tccgttcaca tcgatgactg gaaagcgccc
      181 gactttatct tttgcgaaac gctccatggc tttgataacg ggatcgaaac cattgacggt
      241 gataaccccg ggattcatgt atttgcggac aggcgaactg gtgtcattgg ctgtcagggc
      301 gcggataaga tcttccaggc tgacgatacc gatcagataa ccattttcga cgaccggcgc
      361 gccagaaatg cggtgggtgc gcagcagctc aaccagatcg gagattggtg catccgggct
      421 gagcgcctgc acatcggtcg tcatcacttc ttcgatgcga atatcgtaag ccagctcttc
      481 gacccgggtg atatcgtgcg ctgtatcgtc ggtaaccagc gtacctgtgc gtccgtggat
      541 ttccagattg cttgtcatgg gaattaatct gtaaatttct cttccaggct gggaaggccg
      601 gcagtataca ggcgtccgca gagctcaaac atgccgtggg ggctgctgat cagagggatt
      661 tgctcgcgtt cggcgacttc aacgacatct ggagtaggcg atttcccgcg cacaaagatg
      721 attgcccgga tatcagccat ctgggccgtc cggatggttt ggggattgtt taatccggtg
      781 aggagtatgc tttcaggctg cgctgtcgcc aggacatcgc tcattaaatc tgcgccgcag
      841 gcaccagtaa tctgaaggtc tttaaccggg gggggtgtac aaaaaaaacc gtcaacgata
      901 tttgcgatct ccgatagttt cattctctgt gtggtccagt ctgtcttcgc tggctaaaat
      961 attttccatc cgctctggat gggtgtagac gttgaactga ttgcgccggg cataaccgat
     1021 cagggtaatc ccctggcgct ctgccatctg gatcgataac gagctgggag aggtgcgcga
     1081 gatcaggatt gaggcgttga tccgtccggc tttctggagc atttctgagc tgatccgccc
     1141 ggtggtaagc aggatgcgcc gctgtggctg caaactcgcc atcaacagct tgccagcgat
     1201 cttatccagg gtattgtggc gcccgatatc ttcggcagtg atcaggatct gctcgccatc
     1261 gctcagggcg gaagtgtgta cgccgccgga tttacgatac aaatcctgaa cgtcaaacag
     1321 ccgggcgacc agctggcaca ctttttgagg cgaaataatg ccgccattga cgaccggccg
     1381 gcctttatac tcggtgctga tcgaggtgac ccctccagtg cagccggttg tccgccgcca
     1441 ctggcgcggt ttttcaaccg cctgggtcag ccagacatcg acattttcct ggttgtcaca
     1501 cacgcgcaca tcggcgacat cttcggtccc ctggataaag ccttcattgt acaggaaacc
     1561 ggttgccagg gcttctaaat cgatgggtgt gcacatcagg gtcagccaga cttcaccatt
     1621 aacggtcagg gaaacgggct gctcggtgat cacctcgctg ctgagcggtt tccagatgcc
     1681 agaaacgtac tgctggtact ggacagtctg gttgggggtt ggcaagggtt cattcataat
     1741 tttatattct caaacaagat aaactcactg attattgtat caaaattgaa cctcacagat
     1801 gcgaacaaaa gtcacctggt ttatgtgtca catggcctca tatccaaccc gtccccgctc
     1861 aaatcattgg atctacgctt gagcggctct atattctccg ggcaagctgc ttatgttatg
     1921 attggcacga acaatgcttt agggatcaaa accaggagga taaatatgga tcgctatcgg
     1981 gtaaagccgg gacagaaaat taagctcgat aaatgggacc caaacgatag aagcctgtat
     2041 gatctggata aagaccaggg tgaggaggcg ctggacaagc tcaacgatca actggagaag
     2101 ctccaggagc tgctgtatgt cgagggcaag cacaaaatat taatcgtcct gcagggaatg
     2161 gataccagcg gtaaggatgg agtcatccgg cacgtatttg atggcgtgaa tccgcaggga
     2221 gtgcgtgtag caagcttcaa agtgccgact ccggaagagc tgggccatga ttacctgtgg
     2281 cggatccaca agcagacccc tcaaagaggt gagattgtga tcttcaaccg cagccagtat
     2341 gaagatgtgc tcgtagtgcg agtccatggg ttggtgccgg aggaggtctg gaa
//
LOCUS       DATMXK010000014         2146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_146931, whole genome shotgun sequence.
ACCESSION   DATMXK010000014 DATMXK010000000
VERSION     DATMXK010000014.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146931"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            13..>2146
                     /locus_tag="VN363_00330"
     CDS             13..>2146
                     /locus_tag="VN363_00330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82977.1"
                     /translation="MTDTVVGTGAPIPAAFTAELRRSGGFLGEADYSVARTLVLPSSG
                     SPPYLPVTLDFGEVPVTGTETFSLRFVSIGPRGNTMYFEVFGIGNDRCANVMETDEND
                     VAAPTERGDPAGFKVLVTSPADLNIASAFTTTPPSLDGSIGFGEWPGSSLPFENGYLS
                     VVNDSIRLYVLLNVRGDVVDDRTGDYFWLTFDVDGDGVIDANLDLNYGLSPATGNMRY
                     SYYLGPSTWTGLQADTFSARAKGFGCFFADGSLQFTFFPFSLTCNRHRVWELGIDLAE
                     IGADPGDTLKVGLRVASNSPAFVNEMPPGFSADFSSLIQVNTSPSPFLTIIPNDLADV
                     HLEADAFELTQAIQDRDNTLPLVWGKTTTARVYVDTDGVFSGQPSKAYLYASQGGVDL
                     PGSPLAKYQTAPTTITRSDLGDTANFQLPAYWTRQSQVDYHARSANMFGGVDVSGSQL
                     INFAFHQDPLVWIVPINTGSNASPVLVSNAEIASQESAMEAAFPVRDIRFVHKPWQVI
                     GPTTVGETIDELNQYFGTVVLAWVLGLIFGGSPPFELPDQVYGFTPSGGGISDPTWYD
                     SGGGWVARGFRGTSGELTMAHEINHNLDRSLDGTWGRHVPGGCGAGGPEGPTWPYAND
                     DLQEVGFDTRLPWQTTASKVTAIPANWPDFMSYCQSGKLPTKWISPYRWTHISDVFVN
                     NVNRNLLNRVDEITEVFYISGTVFPDGTG"
BASE COUNT          386 a          723 c          587 g          450 t
ORIGIN      
        1 attgaagtca ggttgaccga taccgtcgtc ggaaccggtg cccccatccc ggcggcattc
       61 actgccgagc tgcgccggtc gggcggcttt ctgggcgaag ccgattattc tgtggccaga
      121 accctcgtcc tgccatcctc cggttcacca ccctatctgc cggtaaccct cgatttcggt
      181 gaagtccccg ttaccgggac ggaaacgttc agcctgcgct ttgtctccat cggtccccgc
      241 ggcaatacga tgtacttcga ggtcttcggg atcggtaatg accgctgcgc caatgtcatg
      301 gaaactgatg agaacgatgt tgccgcaccc accgagcgcg gcgatcctgc cggcttcaag
      361 gtcctggtta ccagcccggc cgatctgaat atcgcctccg ccttcaccac caccccgccc
      421 agtctcgatg gcagcattgg cttcggcgag tggccgggca gcagcctgcc cttcgagaat
      481 ggctacctca gcgttgtcaa cgattccatc cgcctgtatg tgctgttgaa cgtgcgcggc
      541 gatgtggtcg atgaccggac cggcgactac ttctggctga cctttgacgt ggacggcgat
      601 ggagtgatcg atgccaatct ggatctgaac tacggcctca gtccagctac cggcaatatg
      661 cgctactcgt actacctcgg tcccagtaca tggaccgggc tgcaggcgga tactttctcg
      721 gcccgcgcca aaggtttcgg ctgcttcttc gccgatggca gcctgcagtt caccttcttc
      781 cccttctcac tcacctgcaa ccgccatcgc gtctgggagc tgggcatcga cctggccgaa
      841 ataggtgccg atcccggcga caccctgaag gtaggtttgc gcgttgcctc caattctcca
      901 gccttcgtca acgaaatgcc gcctggattc agcgctgact tcagcagcct gatccaggtc
      961 aatacatccc cctcaccctt cttgaccatc atccccaatg acttggcgga tgtccacctg
     1021 gaagccgatg ccttcgagct cacccaggcg atccaggatc gggataatac cctgccgttg
     1081 gtgtggggca aaaccaccac cgcccgggtc tacgtcgata ccgatggcgt attcagcggc
     1141 cagcccagta aagcctatct gtatgcctct caaggtgggg tggatctccc tggctcgccc
     1201 ctggcgaaat accagacggc tcccaccacc atcacccgct cggacctggg agacacggcc
     1261 aacttccagc tgccggccta ctggacgcgc cagagccagg ttgattatca cgcccgctcg
     1321 gcgaatatgt tcggcggcgt ggacgtctcc ggctcgcagc tgatcaactt tgccttccac
     1381 caggaccccc tggtgtggat tgtaccgatc aataccggct cgaacgccag tccggtcctg
     1441 gtcagtaatg ccgagatcgc ctcgcaggaa agcgccatgg aggcggcctt cccggtgcgg
     1501 gatatccgct tcgtccacaa accctggcag gtcatcggcc cgaccacggt tggtgagacg
     1561 atcgatgagc tcaaccagta ctttggcacc gttgtcctcg cctgggtcct gggtctcatc
     1621 tttggcggca gcccaccctt cgagctgccc gaccaggtgt acggctttac accctccggt
     1681 gggggcatct ccgatcccac ctggtacgac agcggcggtg gctgggtggc ccgcggcttc
     1741 cgcggcacct caggcgagct gaccatggcc catgagatca accataatct cgatcgctcg
     1801 ctcgacggca cctggggccg gcatgtaccc ggtggctgcg gcgcaggtgg tccggaaggc
     1861 cccacctggc cttacgccaa tgatgatctc caggaagtgg gcttcgatac ccgcctgccc
     1921 tggcagacca ccgccagcaa agtcactgcc atccccgcta actggcctga cttcatgtcc
     1981 tactgccagt ctggcaagct gcccaccaag tggatttcgc cttaccgctg gactcacatc
     2041 tccgacgtgt tcgtaaacaa cgtcaatcgc aatttgctta atcgcgtcga tgagattacc
     2101 gaagtcttct atatctccgg cacggtattt ccggatggca ccggcc
//
LOCUS       DATMXK010000015         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_101683, whole genome shotgun sequence.
ACCESSION   DATMXK010000015 DATMXK010000000
VERSION     DATMXK010000015.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101683"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..402)
                     /gene="rsmD"
                     /locus_tag="VN363_00335"
     CDS             complement(<1..402)
                     /gene="rsmD"
                     /locus_tag="VN363_00335"
                     /EC_number="2.1.1.171"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559584.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052913 - 16S rRNA
                     (guanine(966)-N(2))-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0031167 - rRNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (guanine(966)-N(2))-methyltransferase
                     RsmD"
                     /protein_id="HWQ82978.1"
                     /translation="MTGLRIIGGKARGRRLRGVPGDSTRPITDRTKESLFNILGADIQ
                     EAAFLDLFGGTGAVGIEALSRGASFVRFLDNERLAVATIKTNLQTTQLSGQAEVLKMD
                     AFHYLAQPPDRSFDYIYIAPPQYKEMWVKALR"
     gene            complement(412..1416)
                     /locus_tag="VN363_00340"
     CDS             complement(412..1416)
                     /locus_tag="VN363_00340"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWQ82979.1"
                     /translation="MKTSSVLTHRERMEACLSQGKLDRVPVALWRHFPVDDQTPDGLA
                     AATAAFQRSFDFDLVKVTPASSFCLRDWGVHDEWHGDPEGTRDYSTPLIHKPDDWRKL
                     TRLDPYAGHLGDQLTCLRLLVKELGTDVPIIQTIFSPLAQAKNLVGKGNLVAHLRQYP
                     EAVHEGLRIITENTLHFVEATCDTGIDGVFYALQHANYDLLTEGEYSQFGQIYDLQIL
                     EPTRQLWLNMLHLHGSDVMFDLASRYPAQILNWHDQETWPDLAEGKKRFGGVVCGGLS
                     REDGLVLGSPVRITGEAHDAIQETSGERFVLGTGCVTPIIAPYGNLMAARASVENYGS
                     "
     gene            complement(1453..1977)
                     /locus_tag="VN363_00345"
     CDS             complement(1453..1977)
                     /locus_tag="VN363_00345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00369.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA thioesterase"
                     /protein_id="HWQ82980.1"
                     /translation="MEHTHPPKTVSASRITLSQLMQPEHANNQGNVHGGSIMKLVDEA
                     GALACMRHAQQRVVTVAVDQLTFRQPIRIGDLVTLTAEVSYVGRTSLEAEVHVTAENP
                     ITGECTHTNTAYIVFVALGDDGNPVAVPPLIPETDTEIQRMKDGKARQEYRLTQSKLA
                     GSARNLPSPTKKGC"
     gene            complement(1982..2362)
                     /locus_tag="VN363_00350"
     CDS             complement(1982..2362)
                     /locus_tag="VN363_00350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82981.1"
                     /translation="MSAVNPPLVPLYTTYGDVGAILRYPYLFNPRGEWIGTVTAAREV
                     FSIYGTYVGQISKEWRIIRPRSLDSIPPARKPLKNPGSLVPPAHFPLPSLMPELTPNL
                     IDVLEEAPELLPAIDSGEFKPDLD"
     gene            complement(2349..>2855)
                     /locus_tag="VN363_00355"
     CDS             complement(2349..>2855)
                     /locus_tag="VN363_00355"
                     /inference="COORDINATES: protein motif:HMM:NF016060.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GtrA family protein"
                     /protein_id="HWQ82982.1"
                     /translation="ANHPLLQRKVGYPVIRFLTNPAERGRFLRFAVVGVIGAVVDFGT
                     FNFLEKVFNVAPIAASIVSFSAAIVSNFLWNRFWTYPDSRSKPITQQLIQFAIISVMG
                     LIIRTPIFAFMDLKLIDYVQMSSFPSLSASQLESLAHNIALAIAVGVVMFWNFFINRF
                     WTYNDVRS"
BASE COUNT          662 a          753 c          770 g          670 t
ORIGIN      
        1 acgtaaagct ttcacccaca tttctttgta ttggggtggg gcgatgtaga tgtaatcaaa
       61 actccggtct ggcggctggg ctaaataatg gaatgcatcc attttaagta cttcagcctg
      121 tccagataac tgagtcgtct gcaggttggt tttgatcgtc gccacagcca ggcgctcgtt
      181 gtccaaaaag cgcacaaagg aagctcccct gctcaatgcc tcaattccca cggcaccggt
      241 ccccccaaac aaatccagga aagctgcttc ctggatatcg gccccgagaa tattaaacag
      301 ggattctttg gtacgatcgg taattggacg ggtgctgtcg ccggggactc cgcgcagccg
      361 gcgcccccgc gccttcccgc cgatgatccg taaaccagtc atccagttga ctcacgatcc
      421 ataattttcc acactggcgc gggctgccat caagtttcca tatggcgcga taattggggt
      481 cacacacccg gtgcccagga caaaccgctc gccgctggtt tcctggatgg cgtcatgcgc
      541 ttcaccggtg atgcgcaccg gcgagccgag taccagccca tcttcacggc tcaacccgcc
      601 gcaaaccaca cccccaaagc gttttttacc ctcagccagg tctggccagg tttcttgatc
      661 gtgccagttc aggatctgag cagggtagcg agatgccaga tcgaacatga catcactgcc
      721 atgcaggtgg agcatattca gccacaactg ccgggtgggt tcgagaattt gcaaatcata
      781 gatctgtcca aattgcgagt attcgccttc ggtgagcagg tcatagtttg catgctgcag
      841 cgcataaaaa acgccgtcaa tgcccgtgtc acaggtagcc tcaacaaaat gcagcgtatt
      901 ttcggtgatt atccgcaaac cttcatggac agcctcggga tactggcgca ggtgtgctac
      961 caggtttcct ttaccgacca gattcttggc ttgcgccagt gggctaaaaa tggtctgaat
     1021 gatcggcaca tctgtgccga gctctttgac caataatctc aaacaggtga gctggtcccc
     1081 caggtgacct gcgtacgggt ccaggcgggt gagtttgcgc caatcgtcag gtttgtgaat
     1141 tagcggcgtg gaataatctc gcgttccctc cgggtcccca tgccactcat catgcacgcc
     1201 ccaatcgcgc aggcaaaacg acgatgccgg ggtaactttg accaggtcaa aatcgaagct
     1261 gcgttggaag gccgctgtgg cagccgccag gccatcgggt gtctgatcat cgactgggaa
     1321 atggcgccat aatgcgactg gaacccggtc aagcttcccc tgggataagc aggcttccat
     1381 tcgttcacga tgggtcagta cagagctggt cttcatcagt tatgcttctc ctttgctgga
     1441 agattttatt tttcagcagc ctttttttgt tggcgatggt agattccttg cgctccccgc
     1501 cagtttgctc tgtgtcaggc gatactcctg gcgggcttta ccgtccttca ttctttgaat
     1561 ttcggtatcc gtctccggta tcaatggcgg cacagccaca ggattaccat catcacccag
     1621 ggcaacgaag acaatataag ctgtattggt atgggtgcat tcgcctgtga tcggattctc
     1681 agccgtgaca tggacctctg cttccagaga ggtacgcccc acatagctga cttcagctgt
     1741 cagagtaacc aggtcaccaa tccggatcgg ctggcggaag gtcagctggt cgacagctac
     1801 ggtgacgacc ctttgctggg catgccgcat acatgccagt gcgccggctt catcgaccag
     1861 tttcataatc gagccgccgt ggacatttcc ctggttattg gcatgctcag gctgcatcag
     1921 ctgagaaagg gtgatacggg aggcactgac ggttttagga ggatgagtat gttccattta
     1981 tttaatccag atctggctta aactcaccgc tgtcgattgc tggcagcagt tccggcgctt
     2041 cttcgagcac atcgatcaaa ttgggggtaa gctcgggcat cagcgaaggg agggggaaat
     2101 gggctggagg aaccaggctg cctggatttt tgagcggctt tctggcaggc ggaatggagt
     2161 ccagagagcg agggcggatg atccgccatt ccttgctgat ctgccctaca taggtgccat
     2221 aaattgaaaa aacctcgcga gcagctgtca ccgtgcctat ccattcgcca cgggggttaa
     2281 acaggtaagg gtagcgcagg atcgctccta cgtcaccata ggtcgtgtaa agcgggacaa
     2341 gcgggggatt aactgcggac atcgttgtat gtccagaaac gattgatgaa aaaattccag
     2401 aacatcacca cccccactgc gattgccagg gcaatattgt gagccagtga ttcaagctga
     2461 ctggcagaca ggctgggaaa ggaactcatc tggacatagt caataagctt gagatccatg
     2521 aacgcgaata ttggtgtccg gataattaat cccatgacac tgatgatcgc aaattggatc
     2581 agctgttggg tgattggctt tgagcgcgag tccggatatg tccagaagcg gttccagagg
     2641 aaattgctaa caatcgctgc gctaaaggat acgattgagg cggcgattgg ggcgacattg
     2701 aatactttct ccagaaaatt aaaagtcccg aaatcgacca ccgcgccgat tacacctacg
     2761 accgcgaagc gcaaaaagcg ccctctttct gcagggttag ttagaaatcg gatcactgga
     2821 tacccaactt ttctttgaag taagggatgg ttcgc
//
LOCUS       DATMXK010000016         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_112963, whole genome shotgun sequence.
ACCESSION   DATMXK010000016 DATMXK010000000
VERSION     DATMXK010000016.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112963"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..511
                     /locus_tag="VN363_00360"
     CDS             110..511
                     /locus_tag="VN363_00360"
                     /inference="COORDINATES: protein motif:HMM:NF024585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ferredoxin family protein"
                     /protein_id="HWQ82983.1"
                     /translation="MTYIITSLCLRDAGCVEVCPVECIVPGKPQEQWPLFYIDPDTCI
                     DCGACVPECPFEAIFPEDEVPAQYTAKGGELINRLSLSGHYDGTNHHGETVSLETTRA
                     LDAGEVVDLTEDIQSNYDYYTKGPGYAALED"
     gene            complement(602..1471)
                     /locus_tag="VN363_00365"
     CDS             complement(602..1471)
                     /locus_tag="VN363_00365"
                     /inference="COORDINATES: protein motif:HMM:NF028425.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="toast rack family protein"
                     /protein_id="HWQ82984.1"
                     /translation="MKSTRTIFAVVLALPLAALACNLNVNLPEIEYKTTDLVTEAIQV
                     PVPAGSSPVDVTLNFGAGQLNLAGGSAQDLISGSAAYNVPEFKPILTNTAAGVELSSG
                     ELEVNAIPRLANEIENTWNLQLGNTPMNLDINAGAYQGRFDLGGLSLTGLDVADGAAD
                     VEVTFAAPNPVEMETLRYSTGASQVKLEGLGNANFKELIFRGGAGSYTVDFGETVLRD
                     ANIDIEIGISSLRIVVPETANVRVIVGGGLSNVTADGAWTQLNDEYRMEGTGPSLVFD
                     ITMGAGNLELRTP"
     gene            complement(1551..>2151)
                     /locus_tag="VN363_00370"
     CDS             complement(1551..>2151)
                     /locus_tag="VN363_00370"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82985.1"
                     /translation="GKIIFQTRMESTLMPDPYASLPAAWRRKLMWISSGLSILILLIF
                     NTFAAPLTTPAAPLGMISFQLAGTPQRLQALLASWDSAARQIAAFCLGLDYLFMPAYA
                     LAISLADRWVGEGLRQRGLPLAPAGIPLAWGVWLGALLDALENGLQAAMLFGSANPAL
                     PGITRVVAVVKFGLVFAGLGYVFYGLAARLVARSTSPAA"
BASE COUNT          484 a          636 c          590 g          441 t
ORIGIN      
        1 tattaatact attcctcata tcctggtatt gtccaggact gcgaattaag gtacaattag
       61 atcaccaaaa gctcaaatac cttaatctca aagttacgga gggttttcaa tgacctatat
      121 cattaccagc ctttgtttgc gcgatgcggg ttgtgtggaa gtatgcccgg ttgaatgtat
      181 tgtcccaggt aaaccacagg aacagtggcc tctgttctat attgaccctg atacctgcat
      241 tgattgcggc gcatgcgtcc ccgagtgccc atttgaagcc atcttccctg aagatgaggt
      301 tccagcccag tatactgcca agggcggcga gctgatcaac cgcctcagcc tgagcgggca
      361 ttatgatggc accaaccatc acggtgagac agtcagcctc gaaactaccc gtgccctgga
      421 tgcaggcgag gttgtggacc tgactgagga tatccaatcc aattatgact actacaccaa
      481 gggtcccggc tacgctgcac tggaagactg aaatcgcagc ccgctaaaat taaataccac
      541 agggctggct taatcattga gccagccctg tgtcattaac ctcttcggaa ggaacgcagg
      601 ctcagggtgt acgcagctcc aggttgccgg cgcccatcgt gatatcgaac accaggcttg
      661 ggcctgtgcc ttccatccga tattcgtcat tgagctgggt ccaggcccca tcggcagtta
      721 cattggacag tccaccgccg acaatgaccc gcacgtttgc cgtctccggc acaacgatgc
      781 gcaggctgct gatgccgatc tcgatatcga tgttcgcatc ccgcagcact gtttcgccaa
      841 agtccacggt atagctgcca gctccgccgc ggaagattaa ttccttaaaa ttggcgttcc
      901 ccagaccttc cagcttcacc tgggaggccc cggttgagta gcgcagggtt tccatctcca
      961 ccgggttcgg cgcagcaaag gtcacttcta catctgcggc gccatcggcc acatccagcc
     1021 cggtcagaga cagcccgccc agatcaaagc ggccctggta agctccggcg ttgatgtcca
     1081 gattcatcgg cgtgttcccc agctgcaaat tccacgtatt ttcgatctca ttggccaggc
     1141 gggggatggc atttacttcc agctcgcccg aggacagctc gacaccggca gccgtattgg
     1201 tcaggatagg cttgaattcg ggcacattat aggctgctga gccagaaatc aaatcctggg
     1261 ctgagccacc ggccagattg agctgaccgg cgccaaaatt caaggtgaca tccaccgggg
     1321 agctcccggc cggaactggt acctggatcg cctcggtcac caggtcggtg gttttatact
     1381 cgatttccgg tagattgaca ttcaaattgc atgccagcgc ggccagcgga agcgccagca
     1441 cgactgcaaa aattgttcga gtgctcttca tctcggtcct ccactgtaat attttcgttc
     1501 tataccggta tacgctgctc tgggcagata gttgcagaat aagctcacat tcaggctgcc
     1561 ggggaggtgg aacgcgccac cagccgggct gccagcccgt agaagacata ccccagaccg
     1621 gcaaacacaa gcccgaattt gaccacagct acaacccggg tgatgcccgg caaggcggga
     1681 ttggcgctgc cgaagagcat ggcagcctgg agcccatttt cgagggcatc cagcagagcc
     1741 cccagccaca cgccccaggc cagcggaatc cctgccggag ccagcggtaa gccgcgctgg
     1801 cgcaagccct cccccaccca gcggtctgcc aggctgatcg ccagggcata cgccggcatg
     1861 aagaggtaat ccaggccgag gcagaacgcc gcgatctggc gagcggcgct atcccagctc
     1921 gccaggagcg cctggagccg ctgaggcgtg cctgccagct ggaatgagat catcccgagg
     1981 ggtgcggctg gcgtggtcag cggcgcggcg aaggtattga agatcagtaa aatcaggatg
     2041 gacaggccgc tggaaatcca catcagcttg cgccgccagg cagccgggag actggcataa
     2101 ggatcaggca tcagagtact ttccattctg gtttgaaaga tgatcttacc t
//
LOCUS       DATMXK010000017         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_56188, whole genome shotgun sequence.
ACCESSION   DATMXK010000017 DATMXK010000000
VERSION     DATMXK010000017.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56188"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2036)
                     /locus_tag="VN363_00375"
     CDS             complement(<1..>2036)
                     /locus_tag="VN363_00375"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWQ82986.1"
                     /translation="SSLAMHYESAGQPERAQKYFEHAARSALNHCANTEAEGYLHSAL
                     RLSEEKRTQAGLYDLLGETYYRQGNFTESIAAWRSGIEIYKELGDLDAMARLYAHAGR
                     AAAPGVGTVVSRSIEITQEGLAAMAGAPDSVGLAMLIHEAGRAYLFNGMGEKAFPLVK
                     QALAMAESLNAVDVQADSLATLGLLPGQPPHEIQAAFERAIELSEKAGLLQIAYRANV
                     NLGSHIQEVYGDPYRAREHYQKATELAVRRGALMEEFLSRTAYASTTLDMGELDEVER
                     LLPSLKLLLSQTSDPGSKDWSLRMLEAWLIGHRGDWARTRDLMAVIRAETMEHSEIIA
                     QGEYGMMWIVANLEYNRFHPISDWAPVEAVLEEVIKSQAISNLPHNLAPLIACHVYAR
                     QGKVTEARQWRDKLRSELHPDEFFANKLDLMACEAAVAAAERSYTQAIEIYTELIDLF
                     ERAKGPLGRAYGLIDLAEVYLLRNQEDDSEQARQLLEQARDLFYGMHAKGNAANIDHR
                     LEVLSQSQSVSLRSVVRELSDAGKIQASFLPDQLPTLEGWDLAVEFNPARQTSGDFYD
                     FIPLPDGKLAIIVADVSDKGAGAALFMTLSRTVLRTYIQLVPDDPALALREASARILA
                     DSHSGMFVTVFYGLLDPPTGRLRYVNAGHNPPYLYRPSAEEPFSTLNRTGMPLG"
BASE COUNT          399 a          622 c          595 g          420 t
ORIGIN      
        1 ccccagcggc atgccggtgc gattcaaggt gctgaacggc tcctccgcac tcgggcggta
       61 caggtaaggt ggattatgcc cggcgttcac gtagcgcagg cgtccggtgg gcgggtcgag
      121 caagccgtaa aagacggtca caaacatccc ggaatgactg tctgccagga tgcgggcgct
      181 ggcttcacgc agcgccaggg ccggatcatc cgggacgagc tggatatagg tgcgcagaac
      241 ggtccggctg agggtcataa acagggccgc gccagcgcct ttatcggaca catcagccac
      301 gataatcgcc agcttcccat ccggcagcgg gataaaatcg tagaaatcac ccgaagtctg
      361 gcgggcggga ttaaattcga cagccagatc ccaaccttcc agtgtgggaa gctggtcggg
      421 caggaagctg gcctggattt tcccggcatc cgaaagctcg cgcaccacac tgcgcaagga
      481 aaccgattgg ctttgtgaga gaacttccag gcggtgatcg atattggcgg cgttaccctt
      541 ggcgtgcatt ccgtagaaca aatcccgcgc ctgctccaat aactggcgag cctgttcgct
      601 gtcatcttcc tggttgcgca gcaggtatac ttctgcgagg tcgatcaggc cgtacgcccg
      661 gcccagcggg cctttggcgc gttcgaagag gtcaatcagc tcggtataaa tctcgatcgc
      721 ctgggtatag gaacgctcag ctgccgccac cgcagcctcg caggccatta aatccagctt
      781 gttggcaaag aattcatccg ggtgcagctc gcttctaagc ttatcgcgcc actggcgcgc
      841 ctcggttact tttccctggc gggcatatac atggcaggca atcaaaggcg ccaaattatg
      901 cggcagattg gatatcgcct gagactttat gacctcttcc agaacagctt cgaccggcgc
      961 ccaatcagag atgggatgga aacgattgta ttccagattg gcgacaatcc acatcatccc
     1021 atattctccc tgggcgatga tctcactatg ttccatggtc tcggcgcgaa tgaccgccat
     1081 cagatctcgg gtcctggccc aatctccccg gtgcccgatc agccaggctt ccagcatgcg
     1141 cagcgaccag tccttggacc ctggatcgga agtctgggag agcaagagct ttaggctcgg
     1201 cagcaggcgt tcgacctcat ccagctcacc catatccagg gttgtgctgg cgtaggcggt
     1261 gcggctgaga aattcttcca ttaaggcgcc gcgccggaca gccagctcgg tcgctttttg
     1321 atagtgctcg cgcgcccggt acgggtcgcc gtaaacctcc tggatatgcg agccaaggtt
     1381 gacgttcgcc cgataggcga tctgcagcaa accggctttt tctgagagct cgatcgcccg
     1441 ctcaaaagct gcctggatct cgtgcggagg ctggccaggc agcaaaccca gcgtagccag
     1501 cgaatccgcc tgcacatcga ctgcgttcag gctctctgcc atggccaatg cctgcttcac
     1561 cagcgggaag gctttttccc ccatcccgtt gaacaggtat gcccggccag cttcgtggat
     1621 caacatcgcc aacccgacac tatcgggtgc ccccgccatc gccgccagac cttcctgggt
     1681 gatctcgata ctcctggata cgacggtgcc aaccccgggg gcagccgctc gtccggcgtg
     1741 cgcatacagg cgcgccatgg catccagatc ccccagctct ttgtaaattt cgatccctga
     1801 gcgccaggca gcgatcgatt cggtgaaatt tccctggcga tagtaggtct ctcccagtaa
     1861 atcgtacagc cccgcctggg tgcgcttttc ttcagacagc cgcagcgccg agtgcaggta
     1921 gccttctgcc tcggtatttg cgcagtggtt cagggcgctg cgggcggcgt gctcgaagta
     1981 tttttgggct cgttcgggct gtccggcgct ttcgtaatgc atcgccaggg aggagg
//
LOCUS       DATMXK010000018         2466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_56187, whole genome shotgun sequence.
ACCESSION   DATMXK010000018 DATMXK010000000
VERSION     DATMXK010000018.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_56187"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VN363_00380"
     CDS             <1..353
                     /locus_tag="VN363_00380"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HWQ82987.1"
                     /translation="INGDTVNDLNDEYILIANGGNDDVNLEEWWINSEFITRGVFDYQ
                     IPDNFILDTDDSVKIWTKSGTNNNSNLYMAKATPVWRNSGDCGYLRDNTDDHELVDRW
                     CYGNLANRNLEPLP"
     gene            complement(434..1291)
                     /locus_tag="VN363_00385"
     CDS             complement(434..1291)
                     /locus_tag="VN363_00385"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWQ82988.1"
                     /translation="MSHSTESHRSTFHCPRCGRLISYHARSCLYCKLPRPSLFANLPL
                     LRELMHGDMRLGEGIFITCIALYFTAILLDIGGSLNSSGLFGLLAPSSGSLYRLGMGG
                     AIPWNLGRWWTLVTATYLHGGLLHIVFNLMWLRSIGPLVEELFGRSRFFIIYTLAGVG
                     GVALSTLMGTPFTVGASGSIFGLLAALIYYGRIRGGTFGAGIMRTMLIYAGIGLVLGF
                     LAPNVDNWGHVGGLLIGFAAAAVLGYEERAKQSLTHHLVASGFLLFVLVAFTIMVVLF
                     FTSPPLPAA"
     gene            1745..2326
                     /locus_tag="VN363_00390"
     CDS             1745..2326
                     /locus_tag="VN363_00390"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HWQ82989.1"
                     /translation="MLIDRIIGAFTFRKGVYAEVEGDASFTTNAWLIVAVAAFLSSLG
                     AAAVSGGSFASMFLGALVGTIFSVIGFGISAFVISWVGVNVFKAQTNFEEVVRTTGLA
                     FVWTAVGVLGILGALASVLACITGPIQFAAWIAGLVAAIFAIKEALDLDWTSTIITVV
                     IGWLVQLVIGLIAGLVLGILGISASGIMGIFAR"
BASE COUNT          565 a          666 c          737 g          498 t
ORIGIN      
        1 tcattaatgg cgataccgtc aacgatctga acgacgaata tatcctgatc gccaacggtg
       61 gcaatgacga cgtgaacctg gaagaatggt ggattaacag cgaattcatc actcgcggag
      121 tcttcgatta ccagatcccg gacaatttca ttctggatac agatgattcg gtcaaaatct
      181 ggacgaagag cgggaccaat aacaactcta acctgtatat ggctaaagcg acgcctgtat
      241 ggcgcaactc cggcgactgt ggctacctgc gcgataacac ggatgatcac gagctggtcg
      301 atcgctggtg ctacggcaat ctcgccaatc gcaatctcga gcctctgcct taatcaggca
      361 ggtaacaaaa ccgggctgca tgccattgca gtccggtttt tttgtttaag cggtcgaaga
      421 agggcgctct gattcaggca gcgggcaagg gcgggctggt gaagaacagc accaccatga
      481 tcgtaaaagc caccaggacg aagagcaaga atccggacgc cacgaggtgg tgggtgaggc
      541 tctgcttggc gcgctcctca taacccaaaa cggccgcggc ggcgaaaccg atcagcaggc
      601 cgcccacatg cccccagtta tccacgttgg gcgccaggaa acccagcacg agaccgatgc
      661 cggcatagat cagcatggtg cgcataatcc cggcgccgaa agtaccgccg cgaatgcgcc
      721 cgtagtagat cagggctgcc aggaggccaa agatcgaccc ggaagcaccc accgtgaacg
      781 gtgtgcccat cagcgtgctc agcgctaccc ctccgacacc tgccagggta tagataatga
      841 agaagcgcga gcgcccaaac agctcttcaa ccaatggacc aatggaacgc agccacatca
      901 ggttgaatac aatatgcagc agcccgccgt ggagatatgt agcagtgacc agcgtccacc
      961 agcgccccag gttccaggga atcgctccgc ccatccccag gcggtacagc gagcctgagc
     1021 tgggcgccag gaggccgaac aggcctgagg aattaagaga gccgccgata tccagcagga
     1081 tggctgtgaa atacagggca atacaggtaa tgaagatccc ctcgccaagc cgcatatccc
     1141 catgcatcag ctccctcagc aggggcaggt tggcaaacag ggaagggcgc ggcagcttgc
     1201 agtacaggca ggagcgggca tggtagctga tcaggcgtcc acagcgcggg cagtgaaagg
     1261 tggagcggtg gctttcggtg gaatggctca tgcggttctc ggcccctggg aggaggataa
     1321 cagtaaggag taagctcaaa atgagtatag catattgcag aaatttctgc aggtcaggct
     1381 ttgaggcagc ggactgaagt ccgctctaca tatgtaggtc ggatttaagg caagtgcatt
     1441 tcttgcctat ccgactccgg cgcagccggg cgcctgttga actggctaat cctgacatcg
     1501 gcaggcaggg attctcgctg gcagggtcac aggtatgcca gcacaagcgg actgaagtcc
     1561 gctctacatg cgtagccggg agcctgcagc ttcgcccacc tcagacaccg gcaggcaggc
     1621 caagcggact gaagtccgct ctacatatga acgacaacta aaattcatca gccgggttat
     1681 aattttctgg aattcccact catccttttt atctgcatat tgaggcaatc taaggaggtt
     1741 caccatgttg atcgaccgca tcatcggagc atttacgttt cgcaaggggg tgtacgctga
     1801 ggttgaaggg gacgcctcct tcaccaccaa cgcctggctg atcgtggccg tcgcggcgtt
     1861 tttgagcagc ctgggcgcag ctgcagtcag cggcggctcg ttcgccagca tgttcctggg
     1921 ggcgctggtg ggcacaattt tcagtgtgat cggcttcgga atttcggctt tcgtgatcag
     1981 ctgggtgggg gtgaacgtat tcaaagccca gacgaacttt gaagaagtgg tgcgcaccac
     2041 cggcctggct ttcgtgtgga ccgcagtggg cgtgctgggc atcctgggcg ccctggcatc
     2101 ggtgctggcc tgcatcacgg gaccgatcca attcgcggcc tggattgcgg gtctggtcgc
     2161 cgccatcttt gccatcaagg aagccctgga cctggactgg acttcgacga ttatcactgt
     2221 ggtcattggc tggctggtcc agctggtgat tggcctgatc gccgggctgg tgctgggaat
     2281 cctggggatc agcgcgtccg ggatcatggg catattcgcg cggtaaacag ttaatattca
     2341 gtggacataa aaccccaggg gttgaaatcc tgcagatttc ttaaacccct ggggtttgtg
     2401 tgtataaact gttttttaga gcagcttttg gccgcactcg gggcagaatt tggcgccttt
     2461 ggtctc
//
LOCUS       DATMXK010000019         2821 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_67783, whole genome shotgun sequence.
ACCESSION   DATMXK010000019 DATMXK010000000
VERSION     DATMXK010000019.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2821)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2821)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2821
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67783"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..982)
                     /gene="xseA"
                     /locus_tag="VN363_00395"
     CDS             complement(<1..982)
                     /gene="xseA"
                     /locus_tag="VN363_00395"
                     /EC_number="3.1.11.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00237.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009318 - exodeoxyribonuclease VII
                     complex [Evidence IEA];
                     GO_function: GO:0008855 - exodeoxyribonuclease VII
                     activity [Evidence IEA];
                     GO_process: GO:0006308 - DNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease VII large subunit"
                     /protein_id="HWQ82990.1"
                     /translation="MQLLPLFEPARWSVSEVTRYIRSLFEEDSQLQDIWVEGEISNFS
                     RPSSGHLYFTLKDSTSSLRCVMWRSQAARVKVLPRDGDAVEVHGSLNVYEAGGQYQLY
                     ADILRPVGQGALYAEFLRRKALLEAEGLFDLERKRPIPHWPHTIGVVTSPTGAALRDI
                     LNTLRRRYPVARVVLAPTAVQGDEAPQGIVQALQNLATHAQPDIILLARGGGSIEDLW
                     AFNDERVVRAVVASPAPVICGVGHETDFTLADFAADLRAPTPTAAAELATPNRIDLLA
                     GLAEIENQLQRNLRVLLSDWRWQLNHTRSRLAGLSPQTRLRSDRQRIDSLE"
     gene            1187..1690
                     /locus_tag="VN363_00400"
     CDS             1187..1690
                     /locus_tag="VN363_00400"
                     /inference="COORDINATES: protein motif:HMM:NF023395.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HIT domain-containing protein"
                     /protein_id="HWQ82991.1"
                     /translation="MNHLWSPWRMEYINNHKTVTGCVFCTALEQPDGPENLIIARGSL
                     AFVILNRYPYTSGHLMVVPNAHQSSLDALDPASRAEIMELTNQTIQVLLNIYHPQGFN
                     LGMNIGSVAGAGIAEHVHMHVVPRWGGDTNFMSAVADTRVLPETLEDTYQRVLAGWQG
                     LLKNNPK"
     gene            1799..>2821
                     /locus_tag="VN363_00405"
     CDS             1799..>2821
                     /locus_tag="VN363_00405"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWQ82992.1"
                     /translation="MSSLHDGEAVILSAVRTPIGRFQGGLSGLPATKLGAIVVEAAVR
                     RAGLTDPADVNEVIMGNVVSAGLGQNPARQAAIFGGLPASVGALTVNKVCGSGLKAIT
                     LAAQGVRAGDGELYVAGGMENMSRAPYLVDGRTGALRYGHAQLTDALLKDGLWDPFEN
                     WGMGDAAEFIAEEFEVSRILMDEYSFRSHQKAIAAIDHGKFKAEIVPVEIAGRKGQVT
                     VVDTDEGPRRDSSLEALQALKPAFQAGGKVTAGNAPGLNDGAAAVVVASRAYAEKHHL
                     SPIARVAGYAQAAVEPKYLFDAPAHALPRLLAKVDWTLDEVDLIELNEAFAAQVLANG
                     AALKSHG"
BASE COUNT          600 a          793 c          863 g          565 t
ORIGIN      
        1 gctccaggct gtcgatgcgc tggcggtccg agcgcaggcg cgtttgggga gagagccctg
       61 ccaggcggct gcgggtgtga ttgagctgcc agcgccagtc gcttaacagg acgcgcaggt
      121 tgcgctggag ctggttctct atctcggcta acccggccag cagatcgatc cggttcggtg
      181 tagccagctc ggcggctgca gtaggagtag gggcacgcag atcggcagca aaatccgcca
      241 gggtgaagtc tgtctcatgt cctacgccgc aaatcacggg cgccggagag gcgacaaccg
      301 cccgaaccac gcgttcatcg ttgaacgccc acaagtcttc gatcgagccc ccgccgcgag
      361 ccagcagaat aatatccggc tgagcatggg tggccaggtt ttgtaaagcc tgaacgatgc
      421 cctggggtgc ttcatcgccc tggacggcag ttggggcaag cacgacgcgg gcgactggat
      481 accggcggcg caaagtattc aagatatccc gcaaggcggc tccggttgga gacgtgacca
      541 ccccgatggt atgcggccaa tgcgggatgg ggcgtttgcg ctccagatcg aataaacctt
      601 cggcttccag cagcgctttg cggcgcaaga actcggcgta cagggcaccc tgcccgaccg
      661 gacgcaggat atccgcatac agctggtact gaccgccggc ttcgtacaca ttcaggctgc
      721 catgcacttc cactgcatcc ccatcgcggg gcagcacttt gacccgggca gcctggctgc
      781 gccacatcac acagcgcaga gagctggtgc tgtccttgag ggtaaagtac aggtgccccg
      841 agctgggtcg ggaaaaattg gagatctccc cctcaaccca gatatcctgc agttgggaat
      901 cttcctcaaa cagggagcga atatagcggg tgacttcgct gaccgaccag cgagcaggtt
      961 caaaaagcgg cagcagctgc ataatacctc aataaaaggg aatgcctggc ggcatatcaa
     1021 tcgggatgag gatactctgc ttcgcaaagc ctgtcaattg ccttctgcac cggtctgctg
     1081 cacccgcttc actgatattt gacccctccg gctacccaac cttttcaacc attccccctg
     1141 ccctgattct ttgctacaat aacttactta cctatggaga ttgcgattga atcatctatg
     1201 gtcaccctgg cgtatggaat acatcaataa tcacaaaaca gtcacaggct gtgttttttg
     1261 cactgccctc gagcagcccg acggaccgga aaacctgatc atcgcccgcg gctcactggc
     1321 ctttgttatc ttgaatcgct acccctatac cagcggacat ttgatggtcg ttccgaacgc
     1381 gcaccagtct tctctggatg cgctcgatcc cgccagccgg gctgagatca tggagctgac
     1441 caaccagaca attcaagtct tgctgaacat ttaccatccc cagggattca atctggggat
     1501 gaatattggc tctgtagcag gggcagggat tgccgagcat gtgcatatgc atgtcgtccc
     1561 acgctggggt ggtgacacaa actttatgtc tgcggtggca gatacgcgcg tgctgcctga
     1621 aaccctggag gatacctacc agcgcgtcct ggccgggtgg cagggcctat tgaaaaacaa
     1681 cccgaaataa ttcactgagc ggcaaaggat gccaaacata aaagcctgcc tggtaagcca
     1741 ggcatcttct gtccagctgt ccaggccatt tggtcagtga caaaaaggag gaggcccgat
     1801 gagcagtttg catgatggtg aggcagtaat cctcagtgcc gtccggacgc cgattgggcg
     1861 ttttcagggg ggcttgagcg ggttgccagc gacaaaactg ggggcgattg tggtggaggc
     1921 ggcggtcagg cgggccggtc tcaccgatcc agcggatgta aatgaagtaa tcatggggaa
     1981 tgtcgtttcc gccgggctgg gccagaatcc ggcccgccag gcggcgatct ttggaggcct
     2041 gccagccagc gtgggggctt tgaccgtgaa taaggtatgc ggctccggcc tgaaagcgat
     2101 cacgctggct gcccaggggg tgcgcgccgg ggatggcgag ctgtatgttg ccggaggcat
     2161 ggagaatatg agccgggcgc cgtatctggt ggatgggcgc accggtgcct tgcgctatgg
     2221 gcacgcccag cttacagatg ccttactcaa agatggattg tgggatcctt tcgaaaattg
     2281 ggggatgggc gatgcggctg aattcattgc tgaggaattt gaagtaagcc gcatcctgat
     2341 ggacgaatac tccttccgca gccatcaaaa ggcgattgct gccatcgacc atggtaaatt
     2401 caaggcggaa atcgtcccgg tggagattgc cgggcgcaag ggccaggtaa cggtggttga
     2461 taccgatgag ggcccgcgcc gggacagctc gctggaggcc ttgcaagcgc taaaacctgc
     2521 attccaggct ggcggcaagg tgaccgccgg gaacgcccca ggtttgaatg atggggcagc
     2581 cgcggtggtc gttgctagcc gcgcctacgc tgaaaaacac catctctcac ccatcgcccg
     2641 cgtcgccggc tatgcccagg cagccgtaga gccaaaatat cttttcgatg ccccggcgca
     2701 tgccctgcct cgtttactgg cgaaagtgga ctggacgctg gacgaggtgg atctgattga
     2761 gcttaacgaa gctttcgctg cccaggttct ggcaaatggg gcagccttga aatcgcacgg
     2821 c
//
LOCUS       DATMXK010000020         3297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_125062, whole genome shotgun sequence.
ACCESSION   DATMXK010000020 DATMXK010000000
VERSION     DATMXK010000020.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125062"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(98..643)
                     /locus_tag="VN363_00410"
     CDS             complement(98..643)
                     /locus_tag="VN363_00410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82993.1"
                     /translation="MKQPYFSTFQLILLAFFAGLIVIAKIALKLPLQAPGHSGLFWMA
                     IIVVASQVVPKPGAASLAGLTSGILSAFLGMGDLGALNTFFSYTAVGVFTDLGLLILR
                     SPENLVNAALIGVIGHLGKFVVKWIFGVVAGAPVGFVALGLVWSFVGYVGWGALGGIL
                     GSLTLKALRKAGFFAYLLEKR"
     gene            complement(640..1398)
                     /locus_tag="VN363_00415"
     CDS             complement(640..1398)
                     /locus_tag="VN363_00415"
                     /inference="COORDINATES: protein motif:HMM:NF014420.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="energy-coupling factor transporter transmembrane
                     component T"
                     /protein_id="HWQ82994.1"
                     /translation="MSTFKPALQRRRSRSFAPAIVESSPLRRVDPRAKLALGLCASLA
                     VMLPLERMLSFIGLAVVFLAWARLLPAALQQVWRLKWVLLFLFLGDWLLVGLDLAILI
                     TLRLALLAAVMVLFFATTTSREFGLALEALHMPYRYAFSLSLSFTSLDMLREEWLAIQ
                     EAQQARGIQPPGGSLRRLIQNVRDFIAFTVPAVVLTTRHAWALTEAAYARGFDSPLRK
                     PYLTLHFTLRDAVLLLGAFSITIFLFIWKSGGIQ"
     gene            complement(1385..2878)
                     /locus_tag="VN363_00420"
     CDS             complement(1385..2878)
                     /locus_tag="VN363_00420"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ82995.1"
                     /translation="MPPYFELSDLSFTYPQAARRGLPPISLQASSGEALLLSGPSGSG
                     KSTLARCLTGLIPHLYHGELRGQAWIDGLPTAETPLWQLSQRAGLVFQNPAAQMLAPT
                     AAEEILFGLENLGLTHAEMQRRLDETLECFGLGEFRTRAPRTLSGGEQQKLALAAILA
                     RQPAGLVLDEPLSMLDSTSAQNFVSLLGAQAAAGRAVIVCEHRHEYLAALPGLRTIRL
                     NGAASNSSSLDISPGLPPVDDLLPYPSIATASEPVRLNLHGVSARRGSTLVLDGLDLE
                     LHGGEVTALVGPNGAGKTTLLRLLAGFQPYTGSLDVSDGVLAPDTVAAQGAVAAQGAA
                     PRLGLVFQNPDLQLFNASVRDEILYRLPHPEPELYTWLLTALGLTPYEDTPPLLLSEG
                     EKRRLALAMVLIHRPVHGLLLDEPSLGQDARHKHTLIHALQAVARSGVVVLMATHDLE
                     LAARADRMLLLSRQGILADGPPARVLADSAAWVELGLIVPEWMHVHV"
     gene            complement(2882..>3297)
                     /locus_tag="VN363_00425"
     CDS             complement(2882..>3297)
                     /locus_tag="VN363_00425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ82996.1"
                     /translation="LCVAADVPEEIIAFGRRAQKEMVDQYLARQLAASHDILLQGLGG
                     NQGGVIGALAAVGLAASGNDGRYIMVGKLRELSGEMPIASILAAGVSAVETLQGEPVS
                     DILLQADKLRPSCRNGLAVQYVAYQDGIWTPLKLD"
BASE COUNT          715 a          927 c         1169 g          486 t
ORIGIN      
        1 cgatcagggc gatcgctaaa acagcaatca ggatgcgttt ggattgggtc atgttttcct
       61 ccaggaattc gtggaatacg cagcccttga gaggggatca gcgtttttcc agcagatagg
      121 cgaagaatcc ggccttacgc aaagccttga gggtgaggct gccgaggatg cctcccagag
      181 cgccccatcc gacatacccc acaaaactcc acaccagacc gagggcgaca aagccgaccg
      241 gcgcgccggc aacgacgcca aagatccatt tgaccacgaa ttttcccagg tgtccgatca
      301 caccaatcag agcggcattt accaggttct cgggagaacg caggatcagc agccccaggt
      361 cggtgaagac accgacggcg gtgtatgaga agaacgtgtt gagcgcgccc agatcaccca
      421 tccccaggaa agcagacaga atgccggagg tgaggccggc caggctggcg gcgccgggtt
      481 tgggcaccac ctggctggcg acgacgatga tcgccatcca gaacagaccg gaatgaccgg
      541 gcgcctgcag ggggagcttg agggcgatct tggcgatgac aattaatccc gcaaaaaagg
      601 ccagtaagat caactggaat gtggagaagt agggttgttt cattggattc cgcctgattt
      661 ccagatgaat aagaatatgg taattgagaa agcacccagg agcagcacgg cgtcgcgcag
      721 ggtaaagtgc agggtgaggt agggcttgcg caggggcgag tcgaagccgc gggcgtaggc
      781 agcttcggtg agcgcccagg catgccgggt cgtgagcacg accgccggga cggtgaaagc
      841 gatgaaatca cgcacattct ggatcaggcg cctcagcgaa ccaccgggcg gctggatacc
      901 gcgcgcctgc tgggcttcct ggatggcaag ccattcttca cgcagcatat ccaggctggt
      961 aaaggacagg ctgaggctga aggcatagcg ataaggcatg tgcagggctt caagcgccag
     1021 accaaactca cggctggtgg tggtggcgaa gaagagcacc atcacggcag ccaaaagggc
     1081 cagacgcaga gtgatcagga tagcgagatc cagcccgacc agcagccaat caccgaggaa
     1141 caggaacagc agcacccact tcaggcgcca gacctgctgt aaggcggccg ggagcaggcg
     1201 cgcccaggcc agaaagacga ctgccaggcc gataaaagac agcatgcgct ccagcggcag
     1261 catgacagcc agcgaggcgc acaggcccag ggctaatttg gcacgcgggt cgacccggcg
     1321 caggggggag gactcaacga tggccggcgc gaaggagcgg gatcggcggc gttggagggc
     1381 tggcttaaac gtggacatgc atccactccg gtacgatcag gccgagctcg acccaggcgg
     1441 cggaatctgc caggacccgg gctggaggcc catctgcgag aatcccctgg cgggagagga
     1501 gcagcatgcg gtctgcccgg gcagccagct ccaggtcgtg ggtggccatg agcacgacca
     1561 cgccgctgcg ggcgacagcc tgcagggcat ggatcagggt gtgcttgtgg cgggcgtcct
     1621 ggcccaggga aggctcatcg agcagcaagc cgtggaccgg gcggtggatc aggaccattg
     1681 ccagggccag gcggcgtttt tcgccctcgc tgagcagcag gggcggggtg tcttcgtatg
     1741 gcgtgagacc cagggcggtt aaaagccagg tataaagctc gggttcagga tgggggagcc
     1801 ggtacaggat ttcgtcgcgc acactggcat tgaagagctg cagatcggga ttctggaaga
     1861 cgaggccgag ccggggggcg gcgccctgag cggctacggc gccctgagcg gctacggtgt
     1921 ccggagcgag tacgccatca gaaacatcca gactgccagt ataaggctga aaaccagcca
     1981 gcaagcgcag cagggtggtc ttgccggcgc cattcggacc caccagggcg gtgacttccc
     2041 cgccgtgcag ctcgagatcg agcccgtcca ggacgagggt ggagccgcgc cgggcgctta
     2101 cgccatgcag attcaaccgc actggctcgg aggcagtagc gatcgagggg taaggcagca
     2161 ggtcgtccac aggggggagg ccgggagaaa tatcgaggga agagctgtta gaggcggcac
     2221 cgttgagccg gatggtgcgc aggccgggca gagcagccag gtattcatgg cggtgctcgc
     2281 agacgatcac ggcgcgcccc gcggcagcct gggcgccgag cagggagacg aaattctggg
     2341 cgctggtgga atcgagcatc gatagtggct cgtccagcac caggccagct ggctggcggg
     2401 caaggatggc tgccagggcc agcttctgct gctcgccgcc ggaaagggtg cgtggggcgc
     2461 gggtgcgaaa ttcgcccaga ccgaagcact ccagggtttc gtccaggcgg cgctgcatct
     2521 cggcgtgggt gaggccgaga ttttcgagcc cgaaaagaat ttcttcggca gccgtgggag
     2581 cgagcatctg ggcggcggga ttctggaata ccagcccggc gcgctgggaa agctgccaga
     2641 gaggtgtctc agcggtcggt aaaccatcga tccaggcctg accgcgcagc tcgccgtggt
     2701 agaggtgggg aatgaggccg gtgagacagc gcgccagggt gcttttaccg gagccagagg
     2761 ggccggaaag gagcagggct tcgccggagc tggcctgcag ggagatgggc ggcaggccgc
     2821 ggcgggcggc ctgtggatag gtaaaggaga ggtcggaaag ctcgaaatag ggaggcacga
     2881 tttaatcgag cttgagaggg gtccagatac catcctgata tgccacgtat tggactgcca
     2941 ggccattgcg gcaagagggg cggagcttat cggcctggag caggatgtca ctgaccggct
     3001 cgccctggag ggtctccacg gcgctgacgc cggcagccag gatggacgcg atgggcatct
     3061 caccggagag ctcgcgcagc ttgccgacca taatatagcg gccatcgttg ccgctggcag
     3121 ccaggccaac cgcagccaga gcgccgataa cgccgccctg attcccgccc agcccctgga
     3181 gcaggatatc gtggctcgca gccagctggc gggccagata ctggtcgacc atctcttttt
     3241 gggcacgcct gccaaaggcg ataatctctt cgggcacatc ggccgccacg cacaggc
//
LOCUS       DATMXK010000021         3317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_159213, whole genome shotgun sequence.
ACCESSION   DATMXK010000021 DATMXK010000000
VERSION     DATMXK010000021.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3317
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159213"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VN363_00430"
     CDS             <1..411
                     /locus_tag="VN363_00430"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWQ82997.1"
                     /translation="DYGWPGCHAGRLVDPEFGSEQSCASVEKPVAEIQAHSAPLGLSF
                     YAGDRFPPEYQGDLFVALHGSWNRSEPTGYKVVRIPLDGGTAGPVEDFASGWLVDGDS
                     WGRPVDVITGPDGALYISDDGAGMIYRVDYLGSQ"
     gene            432..2975
                     /locus_tag="VN363_00435"
     CDS             432..2975
                     /locus_tag="VN363_00435"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF87 domain-containing protein"
                     /protein_id="HWQ82998.1"
                     /translation="MDTPGQFYLGRMVDPATGKAGSEPLIYDSEDLTTHGVVVGMTGS
                     GKTGLCIGMLEEAALNHIPALMIDPKGDITNALLHFPNLSPQDFQPWVNPDQARREGK
                     TLAQAAEETAANWRKGLEDWGIGPERLQKLADSAHFAIYTPGSDSGIPVSILASLKAP
                     VIPWDSNREMLREQISSTVTALLGLVGLKDIDPVRSREHILLSNIFEYAWSHGHDLDL
                     SELILQAQTPPFQKLGVFDVNTFFPEKDRFQLAMLLNNILAAPAFQSWIEGQPLDVQE
                     LLYHPDGKPRHSVFYIAHLSDEERMFFVTLLYSAVETWMRSQTGTGQLRAIVYFDEIF
                     GYLPPVANPASKQVMLRMLKQARAFGVGQVLVTQNPVDVDYKALSNAGSWFIGKLQTD
                     QDKQRLLDGLSSAAGAGMDRAQYDHLISGLGKRVFLLHNIHNKQPVLFSTRWAMNYLA
                     GPLTRSQIPPLNAMVGATAPAASTPETPQQAATLTTNSKPPAARPAAATVTPASMVTG
                     ANRLGSLTRPGVPAGYQEYFLPNNVTLSQAFQASGRSYPENFASQGLIYKPVLLAQAG
                     VRFFNRKYNLDTELNHTALVTDPDRRGVVRWEDYSADPIAPSALERQPAPESLYASLE
                     APLSDAKLLKVLEADFTEWAFRSSQVTVRVNENLNVYAGPEVSPADFRALCAEAARAG
                     RDAELKKLSDQYDRKLTTLKDKLTRESRELDQDEDELSSRKMEELGTHADTIFSLFGK
                     RSSRRKVSSSMTKRRLTSQAKADVTESKEVIADLTQQISALEAEKNRAVQEVNDHWAE
                     LVNRVGEIPVSPMKKDVLVSLFGIAWLPYHLVEAGSERFELPGYRVSAG"
     gene            3053..>3317
                     /locus_tag="VN363_00440"
     CDS             3053..>3317
                     /locus_tag="VN363_00440"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWQ82999.1"
                     /translation="MKKLLILRHAKSSWDHPDLADIDRPLNKRGLRDAPRIGHWLKHQ
                     ELVPDVIASSPADRAKTTARLVAEACGFSQPVKQVADFYGGGPE"
BASE COUNT          735 a          874 c          942 g          766 t
ORIGIN      
        1 gattatggct ggccgggctg ccatgctggc cgccttgtcg atcccgaatt cggatcggaa
       61 cagtcctgtg cctcagtcga aaagcccgtg gcggaaatcc aggctcattc tgctccactt
      121 gggttgtcat tttatgccgg ggatcgcttt ccacctgaat atcagggcga cctgtttgtc
      181 gctttgcatg gctcgtggaa caggagcgaa ccaacgggtt ataaagtcgt ccgtatccca
      241 ttagatggtg gcacggcagg tccagtcgag gactttgcat ccggctggct ggtagatgga
      301 gattcctggg gtcgcccggt cgatgtaatt actggcccgg atggtgcgtt atatatatca
      361 gatgatggcg cgggtatgat ttatcgggtc gattatctgg ggtcacagta atctttcgga
      421 aggaaaagag tatggatact cctggacagt tttacctggg acgaatggtt gatcctgcaa
      481 ctggaaaggc cggcagcgag cccttgattt atgattccga agacctgact acccacggtg
      541 tagtggtggg tatgaccggg tcggggaaga ccggcctgtg tattggaatg ctggaagaag
      601 ctgccctcaa tcatatcccg gctttgatga tcgatcccaa gggcgacatc accaatgctt
      661 tgctgcattt cccgaatcta agtccgcagg acttccagcc gtgggtcaat cctgaccagg
      721 ctcggcggga aggaaaaact cttgcacagg ctgctgaaga gaccgccgcg aattggcgta
      781 aaggattgga ggattggggc atcggtccgg aacgcttgca gaagctggct gattcagccc
      841 attttgcgat ctatacgccc ggctctgatt cgggcatccc ggtaagtatt ctggcatctt
      901 tgaaggcacc tgtgattccc tgggacagca accgtgagat gctgcgcgag cagatttcca
      961 gcacggtcac tgccttgttg ggtctggtcg gtttgaaaga cattgatccg gtgcgctcgc
     1021 gcgagcatat tctgctgtct aatattttcg aatacgcctg gagccacggg cacgacctgg
     1081 acttgagcga gttgatcctg caggctcaaa caccgccatt ccaaaaatta ggagtgttcg
     1141 atgtcaatac tttcttccct gagaaagacc gcttccagct ggcaatgctg ctcaataata
     1201 ttctggcagc ccctgctttc cagtcctgga tcgaaggcca acccctggat gttcaggaat
     1261 tactctacca cccggatggc aaaccgcgcc acagcgtctt ctatattgct cacctgagcg
     1321 atgaagaacg catgtttttc gtcaccttgc tttattcggc tgtagaaacc tggatgcggt
     1381 cccaaactgg gacgggtcag ctgcgggcca tcgtttattt tgacgagatt tttggatact
     1441 tgccacccgt tgctaatcca gcttcaaaac aagtcatgct gcgcatgctc aagcaggcgc
     1501 gggccttcgg ggtgggtcag gtccttgtca cccaaaaccc ggtcgatgtc gactataagg
     1561 ccctttcaaa tgccgggagc tggtttatcg gcaagctgca aaccgaccag gacaaacaac
     1621 gcttgttgga tggtctgtcc agcgctgcag gtgccggcat ggatcgcgcc cagtatgacc
     1681 atttgatttc gggtctgggg aagcgagtgt tcctgctgca taatattcac aataaacagc
     1741 ccgtactgtt ctcaacccga tgggcgatga actatctggc aggaccgctc acacgctccc
     1801 agatcccgcc tttaaatgcg atggttggcg caacagctcc ggcagccagc actccggaaa
     1861 caccccagca ggctgccacg cttacaacaa actctaaacc acctgctgcc agacctgcag
     1921 ccgcgactgt cacacctgcc agcatggtaa caggggcgaa caggctcggt tcgctgacgc
     1981 gaccgggtgt tcccgccggc taccaggaat attttttgcc caacaatgtc accctttcac
     2041 aggcgtttca ggcctctggg cggagttacc ctgaaaattt tgccagccag ggtttgattt
     2101 acaagccggt gctgctggcg caggcaggag tgcggttctt caatcgtaag tacaatctgg
     2161 acaccgagct taaccatacc gctctggtga ccgaccccga ccggcggggt gttgtgcgct
     2221 gggaggatta ttcggcagat ccaatcgctc cgagcgccct cgaacggcag ccagctccag
     2281 agagcctgta tgcctccctg gaagcgcctc tctcggatgc gaaattgctc aaagttttgg
     2341 aagcggattt tactgaatgg gcgttccgtt cttcccaggt tacggttcgt gtgaatgaaa
     2401 atttgaatgt ttatgccgga ccagaagtat cccccgccga ttttcgggct ttatgtgcag
     2461 aggcagcccg cgctggacgg gatgcggagc tgaagaaatt atccgatcag tatgatcgca
     2521 agctgacaac cttgaaggat aagctgactc gcgaatcccg cgagcttgac caggatgagg
     2581 atgaactttc cagtcgcaag atggaagagc tgggtacgca tgccgataca attttcagcc
     2641 tgtttggtaa acgcagctcg cgacgcaaag tatccagctc gatgaccaag cgccgcctga
     2701 cctctcaggc taaagcagat gtgaccgagt cgaaagaagt tattgctgat ttgacccagc
     2761 agatcagcgc tctggaagct gaaaaaaacc gggccgttca ggaggttaat gatcactggg
     2821 cagaattggt taaccgggtg ggagagatac ccgtatcccc aatgaagaaa gatgtcctgg
     2881 tgagcctgtt cgggattgcc tggctgcctt accatctggt cgaggcaggc tcagagcgct
     2941 ttgagctgcc gggatatagg gtgtccgccg gataattttt ccaggacata tgcgggtgtg
     3001 tacagcgctg accacacctg cttgaggaaa acggtcggga agcgaggcag gcatgaagaa
     3061 actgttgatt ttgcgccatg ctaagtcgag ctgggaccat cctgatttgg cagatatcga
     3121 ccggcctctc aataaacgcg gcttgcggga tgcaccgcgc atcgggcatt ggctcaagca
     3181 ccaggaactt gtccccgatg taattgccag ctcaccagcg gatcgcgcca aaacaaccgc
     3241 tcgcctggtt gccgaggcat gtggcttctc gcagccggtc aagcaggtgg ctgactttta
     3301 tggcggtgga ccggaag
//
LOCUS       DATMXK010000022         2539 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_101410, whole genome shotgun sequence.
ACCESSION   DATMXK010000022 DATMXK010000000
VERSION     DATMXK010000022.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2539)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2539)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2539
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101410"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(100..1176)
                     /locus_tag="VN363_00445"
     CDS             complement(100..1176)
                     /locus_tag="VN363_00445"
                     /inference="COORDINATES: protein motif:HMM:NF017864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="asparaginase"
                     /protein_id="HWQ83000.1"
                     /translation="MQPSDTLPIFELSRAGTVESVHFGAAAVVDSTGKLIAKYGNPDL
                     VTFLRSSAKPFQVLPLLEAGGQERFGFTPREIALMCASHSGTDSHVAVAESIQVKAGV
                     TEADLLCGVHEPTHEPTANSLRERHEAPTPNRHNCSGKHSGMLALGRIYQAVHIDDGS
                     LDYIDNQHPVQKAILHTFADMCTLTTDQIHLGIDGCSAPNFAVPLRNAAYGFARLCDP
                     DTGSVEPLSRREACRTVTSAMMFNPDMVGGPDTFDTTLMELGKGRWVSKGGAEGYQAL
                     GILPGVLGHGSPGLGITLKIADGDQKSRARHAVALAILQQLGILTADELARLGRFGPV
                     SPVLNWRKIEVGIARPLIQLDFSH"
     gene            complement(1219..2094)
                     /locus_tag="VN363_00450"
     CDS             complement(1219..2094)
                     /locus_tag="VN363_00450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83001.1"
                     /translation="MRQIQRGIYFEDSYLGVTLGGLVYAHGTIMIDAPLRPEDARSWR
                     STLLNQRGGSNRLLVNLDSHPDRTLGARSLECAIVTHQKAANVFRTRPSIFKGQSMEM
                     GAMWEDYDDAIGLRWANPDATFTDTMSLHWGGPEIVLESHPGPAIGAIWTTIPEEKVV
                     FIGDAVVLNQPPFLADVEFPAWLEALNHLEQAYADFIIISSRGGLVTLDQVQEQIDYL
                     QKAQQLIQQYIDTKVQPEAMEELIPELIQGFTFPNRWREKYQQRLRYGLYQYHSHHYG
                     SPTLVDSPNVPDTEV"
     gene            complement(2128..>2539)
                     /locus_tag="VN363_00455"
     CDS             complement(2128..>2539)
                     /locus_tag="VN363_00455"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83002.1"
                     /translation="AILPIANNKKQNIEIMIAGDLPQLWIDSDMIRRVLINLLENAVK
                     FTPTEGLLKAGVYQERNGIIFFVEDTGPGIPEVDQQRIFTKFTRLQTSPGLKSFGLGL
                     AFCRLAVEAHGGKIDVQNVPGSGSRFSFCLPVST"
BASE COUNT          629 a          672 c          626 g          612 t
ORIGIN      
        1 gcgggtagtg gatcccgcca ggcaggataa ccataaaact ccagcaggcg ttcgtgaacc
       61 agccgggctt tagccgttaa attcatccaa actcctggtt tagtggctga aatctaactg
      121 tatcaagggg cgagcgatac ctacttcgat tttacgccaa tttaaaaccg gggacactgg
      181 cccaaaccgt cccaacctcg ccaactcatc tgcagtgagt atgcccaatt gctggaggat
      241 agccagggct acggcatgcc gggcgcgcga tttctggtcc ccatcggcga ttttcagagt
      301 aatccccagg cctggtgagc catgccctaa aaccccaggc agaataccta gcgcctggta
      361 accttcggct ccccctttag aaacccatcg tcctttgcct aattccatca aagtagtatc
      421 gaatgtgtcc ggacctccta ccatatccgg attgaacatc atcgctgagg tgactgtccg
      481 gcaagcctcc cgcctggaaa gaggttcaac ggaccctgtg tctggatcac acaatcgggc
      541 gaaaccgtaa gcagcatttc ggagtggaac agcaaaatta ggggccgagc atccatctat
      601 ccccaaatga atctggtcag tggttaatgt gcacatatcc gcgaaagtat gcaggatcgc
      661 tttttggaca ggatgctggt tatcgatata atccagggaa ccgtcatcga tgtggacagc
      721 ctgatagatc cggcccaacg ccaacatacc cgaatgtttt cctgagcagt tatggcgatt
      781 aggggttggt gcttcgtgac gctcccgtaa gctattggcg gttggctcgt gcgtgggctc
      841 gtgtacacca cacaacaaat cggcttctgt taccccggcc ttcacctgga tcgattcagc
      901 cactgcaaca tggctatcgg ttccggaatg agaggcgcac attaacgcta tttcacgcgg
      961 tgtgaaaccg aagcgctcct gaccgcctgc ctccagtaag ggcaatacct gaaagggttt
     1021 tgccgatgag cgtaagaacg taaccaggtc agggtttcca tatttggcga tcaatttgcc
     1081 ggtggaatcg acgaccgcgg cagcgccaaa gtgaaccgat tcaaccgtac cggcgcgact
     1141 cagctcgaaa atgggtagag tatctgatgg ctgcatggca tttgattgag gtgagacttt
     1201 atatatttga gggcctgttt aaacttcagt gtcaggaacg ttcggggaat ccaccaaggt
     1261 gggtgaacca taatgatgag agtgatactg gtatagtcca taccgtaagc gctgctggta
     1321 tttttcacgc caccggttcg gaaaggtaaa tccctggatt aattccggga tcagctcttc
     1381 catggcttcg ggttggactt tggtatcaat atattgctgg atcagctgct gcgccttttg
     1441 taaataatct atttgttcct ggacctgatc cagggtaacc aggccacccc ggctgcttat
     1501 aataataaaa tcagcatagg cctgctccag gtggttgagc gcctctaacc aggcaggaaa
     1561 ttccacatct gcaaggaagg gcggttggtt caatacgacc gcatcgccga taaaaaccac
     1621 cttttcctca gggatagtgg tccagatggc gccgatggcc ggacccggat gagattccag
     1681 aacaatttct ggccctcccc aatgcaggga catcgtatcg gtgaatgtgg catctggatt
     1741 tgcccaacgt aagccaattg catcgtcata atcttcccac atggcgccca tctccatgct
     1801 ctggccttta aatatcgacg gtcgcgtgcg aaacacattg gcggcttttt ggtgtgtaac
     1861 gattgcacac tccaacgagc gtgctcccag ggtacggtcg ggatgtgaat ccaaattcac
     1921 gagtaatcgg ttcgagccac cacgttggtt taacagggtt gagcgccagg aacgggcatc
     1981 ttcaggtcgc aagggtgcgt caatcatgat cgtaccgtgt gcgtagacca gaccgcctaa
     2041 agttaccccc aggtaagaat cttcgaagta tatcccacgc tgaatctgtc gcatagttct
     2101 ctcattcgaa aaggatagcc aacgaaatta cgttgacact ggtaagcaaa aactaaaccg
     2161 cgaaccgctg cccggtacat tctgcacatc tattttccca ccatgcgctt ccactgccaa
     2221 ccggcaaaat gccagtccta atccaaagct tttcaaaccc gggctggttt gcaagcgagt
     2281 gaacttcgta aatatccgct gctggtctac ctcaggaata cccggaccgg tatcctcgac
     2341 aaaaaagatg atcccatttc tttcctggta tacgccagct ttgagcagcc cctccgtagg
     2401 ggtaaatttc accgcatttt ccaggagatt aatcaaaaca cgccggatca tatcactatc
     2461 tatccataat tgcggtagat cgccagcgat cattatctca atattctgct tcttattgtt
     2521 agcaattggc aggattgca
//
LOCUS       DATMXK010000023         4708 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_124930, whole genome shotgun sequence.
ACCESSION   DATMXK010000023 DATMXK010000000
VERSION     DATMXK010000023.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4708)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4708)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4708
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_124930"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2464
                     /locus_tag="VN363_00460"
     CDS             <1..2464
                     /locus_tag="VN363_00460"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ83003.1"
                     /translation="PGGAVQAANRSEIPAIEWTGVPAFEAARQGIPFNVSLTTDSADT
                     SPTTPVALVTVPVSDLSGKLTGVLAAPVDLRKLPFFSAGQRFDLGETGTVDVVDGHGH
                     VLASTDPSRLLTTQEHDSVVNKLFVAGKPGVENCLGCSNDPLSEFSDEVVAFAPLSEA
                     PWGVVIRQKASEVFALVRRLMLVTVILALVTVLGALFLVWMTTNSVIKPVQALTAAAQ
                     RITAGDLDTPITHLSSHQYTRDELGILAQSFVGMRKQLKRSMNEIQGLNQTLDTRVRE
                     RTQAALDAQLEAQAARDDLRAIIDALDDELVVVDLKRNEVQLANRIAQDHHVEHGNLI
                     GMPYFQAFPCMDPDTNGDCQNPIPEVLQTNKVVRSIHYHPANGSSDQRILDIVASPMR
                     ASSGEVTRVVELLRDVTEEHSMRQALLRRNQQLAILNAISTTVNKSLNLEELLNHTLV
                     EVLRLTQVDVGAVFLLEAVLGKLELAACQGLSPEAARLASEMGMLDSSCGGIMDRGEV
                     IVVPDIRSYRGKRARSLKKEHLSSLMHIPLMSKGFTLGSLCLGTRKPIEFTKEEEELL
                     KAIGSQIAVAVENARLYAEVQQKEQIRGELFNKAINAQEDERKRIARELHDETSQTLT
                     AMLFTLDDAAECSDLNEVRQRLSHLRGLSEHTLDGVHKLIFDLRPSMLDHLGLVPALR
                     GYAEACLVNQGVRVVVDETSAPRRLESGVETAIFRVVQEALNNIARHAAARNVWIQLN
                     YNHQCLTVTVEDDGIGFEPSSVQISPSGHPGKGNQRGLGLMGMRERLELLGGTLEITS
                     LPGQGTELVITVPLMEKSVEHV"
     gene            2457..3146
                     /locus_tag="VN363_00465"
     CDS             2457..3146
                     /locus_tag="VN363_00465"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83004.1"
                     /translation="MSEVRVLIVDDHAILRDGIRSMLERYDDIQLVGEAGDGRQALAL
                     VAALKPDIVLMDINMPEMNGLEATRLIVEQHPQTKVLILTQHDNHEYVTSLLQAGAAG
                     YVLKRSGGRDLINAIHQVHDQGAYLEGSVARQLLDRYTSRPASDRDGLLALTERERQV
                     LHLIAQGKSNKEIAQRLVISPKTVSVHRTNIMNKLNVESSFELIRYVTQNQILDENDR
                     TSLPESQPPAA"
     gene            3192..3995
                     /locus_tag="VN363_00470"
     CDS             3192..3995
                     /locus_tag="VN363_00470"
                     /inference="COORDINATES: protein motif:HMM:NF015722.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylglycine-generating enzyme family protein"
                     /protein_id="HWQ83005.1"
                     /translation="MAEYSPLQIEFIQIPAGPMVMGSNSTLDHAAQIDEFPAHELEIT
                     DFYIGRFPVTNAQYRQFVAESGQRPPLFWKDGQLPAGKEDHPVVGVSLNDVLQFCAWA
                     RTRTGLPVRLPTEAEWEKAARGPQGNIYPWGSQWEAGRCNSREARLDSTSPVGQFSPA
                     GDSPYGVADLGGNVQEWLISIFGPYPYDPTDGREELVHVEGEVPRFPQIYETGGTSLP
                     ESMEAALGKATMRGGSYRLKKTECRCAYRSWAAPMHRSDDTGFRVCYEP"
     gene            complement(4265..>4708)
                     /locus_tag="VN363_00475"
     CDS             complement(4265..>4708)
                     /locus_tag="VN363_00475"
                     /inference="COORDINATES: protein motif:HMM:NF018184.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GyrI-like domain-containing protein"
                     /protein_id="HWQ83006.1"
                     /translation="VIEQIEQPVLSIRTTIAVQDMPTVLGQAYGEIMAQIQSQGKYPV
                     GMPFVAYYNMDMQALDIEIGFPTARSLPGKGAVQPSKIPAGPVATCEYTGPYEEMATP
                     YEALQAWIIEHGYEPTGISYEYYLNGPEDAPPSGNKTRIAFPLKA"
BASE COUNT         1008 a         1353 c         1419 g          928 t
ORIGIN      
        1 cccgggcggg gcagtccagg cagccaaccg cagcgaaatc ccggcgatcg aatggactgg
       61 cgtaccagcc tttgaagccg cccggcaggg tatcccattt aatgtcagcc tgacaacgga
      121 cagcgcagat acatctccga ccactccggt agccctggtg accgtcccgg tcagcgacct
      181 gagtggaaag cttacgggtg tgctggctgc accggtagac ctgcggaaac tacctttttt
      241 ctcagcggga cagcgtttcg atctggggga aaccgggact gtcgatgttg tggatgggca
      301 cgggcatgtg cttgccagca ccgatccatc ccgcctgctc accacccagg agcacgattc
      361 tgtcgtgaat aaattattcg tcgccggcaa gcccggcgtc gaaaactgcc tgggctgctc
      421 aaatgatcca ttatccgaat ttagcgatga agtcgtggcc tttgcgccat tgagcgaagc
      481 gccctggggg gttgtcattc gccagaaagc cagcgaagtt tttgcccttg tgcgccgctt
      541 gatgctggtc accgtcattc tggccctggt tacagtactt ggagctctgt ttctggtctg
      601 gatgaccacg aacagtgtga tcaaaccagt ccaggccctg actgcagcgg cccagcgcat
      661 taccgcgggt gacctggaca cgccaattac ccatctctcc agccaccaat acacccggga
      721 cgagctgggc atcctggcgc agagttttgt cgggatgcgc aaacagctca agcgctcgat
      781 gaacgaaatc caggggctga accagacgct cgatacgcgc gtgcgcgagc gcacccaggc
      841 ggcactggac gcccagctgg aagcccaggc ggcccgcgat gacctgcggg caatcatcga
      901 cgccctggac gatgagctgg tcgttgtgga cctgaaacgg aacgaagtcc agctggcaaa
      961 tcgcatcgcc caggaccacc acgtggaaca cggcaattta atcggcatgc cctacttcca
     1021 ggccttcccc tgtatggatc cagataccaa tggagactgc cagaatccca tccccgaagt
     1081 gctccaaacc aataaagtgg tgcggagtat tcattaccat ccagcaaacg gcagtagcga
     1141 ccagcggatc ctggatatcg tggcttcacc catgcgcgct tccagcggag aggtcactcg
     1201 cgtggtggag ttactccgcg atgtgaccga agaacactcc atgcgccagg cattactgcg
     1261 ccgcaaccag cagctggcaa tcctgaatgc tatctccacg accgtcaata agtccctcaa
     1321 tctggaagag ctgctcaatc acacgctggt ggaagtactc cggctgaccc aggtcgatgt
     1381 gggagcagtc ttcttactgg aagcagtact tggcaagctg gagctggctg cctgccaggg
     1441 cctctcgccc gaagccgccc gcctggcatc cgaaatgggc atgctggaca gctcctgcgg
     1501 agggatcatg gaccggggcg aagtgatcgt ggtgccagat attcgcagct accggggcaa
     1561 acgggcgcgc tcgctgaaaa aggagcattt atcctcgctg atgcacattc cattgatgtc
     1621 gaaaggcttt accctgggga gcctgtgcct gggtacccgc aaacccattg agttcaccaa
     1681 ggaggaggaa gaactgctca aagcgatcgg cagccagatc gcggtggcag tggaaaatgc
     1741 ccgcctgtat gccgaggtcc agcaaaaaga gcagatccgg ggcgagttgt tcaataaggc
     1801 catcaatgcc caggaagacg agcgcaaacg catcgcccgc gagctgcacg atgagaccag
     1861 ccagacgctg acggcgatgc tgttcactct ggatgatgcc gcagaatgct cggacctcaa
     1921 cgaagtccgc cagcgattaa gtcacctgcg aggcctctct gagcataccc tcgacggggt
     1981 ccataagctg atctttgatc tgcgccccag catgctcgac cacctgggcc tggttccggc
     2041 gctgcgcggc tatgctgagg cgtgcctggt gaatcaggga gtgcgggtgg ttgtggatga
     2101 aacctctgct ccccgccggc tagagtcggg ggttgagaca gccattttcc gggttgtgca
     2161 ggaggcgctc aacaatatcg cccggcatgc cgccgcccgc aacgtttgga tccaattgaa
     2221 ttacaatcac cagtgcctga ctgtcactgt cgaagatgat gggatcgggt ttgaacccag
     2281 ctcagtccag atcagcccga gcggccatcc tggaaaagga aaccagcgcg ggctgggtct
     2341 gatggggatg cgtgaacgac tggaattact gggcggcacg ttggagatca cctcattacc
     2401 gggccagggg actgagctgg tgatcaccgt tcctttgatg gagaaaagtg tggaacatgt
     2461 ctgaggtgag agtattgatc gtggacgatc atgctatcct gcgcgacggc atccgctcaa
     2521 tgctggagcg ttatgacgat atccagctgg taggagaagc cggggatggg cgccaggctc
     2581 tggctctggt cgcggcacta aagccggata tcgtgctgat ggatatcaac atgccagaga
     2641 tgaacggcct ggaagccacc cgcctgatcg tggaacagca cccacagacg aaagtgttga
     2701 tcctcaccca gcatgacaac catgaatatg tcacttcgct tttgcaggcc ggggcggcgg
     2761 gttatgtgct caagcgctcc ggcgggcggg atctgatcaa cgccatccac caggtgcatg
     2821 accagggcgc atacctggag gggagtgtcg cccgccagct gttggatcgc tataccagcc
     2881 ggcctgccag tgacagagat ggcctgctgg cattaaccga gcgcgagcgg caggtactcc
     2941 atctgatcgc tcagggcaag agcaacaagg agattgccca gaggctggtg atcagcccga
     3001 aaaccgtctc ggttcaccgc accaatatca tgaacaagct aaatgtcgaa agcagcttcg
     3061 agctgatccg ttatgtcact caaaatcaga tcctggacga gaacgatcgt accagcctgc
     3121 cagaaagcca accgccggct gcgtaaagca gacctccagt ctggctcatc caatcctgaa
     3181 ggagtttgtt gatggctgag tattccccgc tgcagatcga atttatccaa atcccggccg
     3241 gaccaatggt tatgggcagc aattcgaccc ttgaccacgc tgcccagatc gacgagtttc
     3301 ccgcccacga gctggagatc acggatttct atatcggtcg ctttcccgtg accaatgccc
     3361 agtatcgcca gttcgtcgca gagagtggac agcgacctcc gctcttctgg aaggacgggc
     3421 agttgccggc cgggaaggaa gatcaccctg tggtgggggt ttcgctcaac gatgtcctgc
     3481 agttctgcgc ctgggcgcgc accaggaccg gcctgccggt acgcctgccg actgaagcgg
     3541 aatgggagaa ggcagcccgc ggaccgcagg gaaatattta cccctggggc agccagtggg
     3601 aggccgggcg ctgcaacagc cgcgaagcca gactcgacag caccagcccg gtgggtcaat
     3661 tctcaccggc cggcgacagc ccgtacggtg tggcagacct gggcggtaat gtgcaggaat
     3721 ggctgatttc catctttggc ccgtaccctt atgatccaac cgacgggcgt gaggagctgg
     3781 tccatgtgga gggcgaggta cctcgcttcc cacagatcta tgagaccggg ggaacatcgc
     3841 ttccggaatc gatggaggct gctctgggta aggcgacgat gaggggtgga tcgtaccgcc
     3901 tgaagaagac tgagtgccgc tgtgcgtacc ggtcctgggc ggctcccatg catcgcagcg
     3961 acgataccgg atttcgcgtt tgctacgagc cgtagagtaa ccgtgtagag cggatttcaa
     4021 tccgcttgtg caggcaggct aaggctcaca tccaactcac tgacctgcct gcccgagatt
     4081 gggttgagcg aagctgcagg cgtccggctg cgcatggaag cggacttcag tccgcttgga
     4141 ctggcatacc cgtgaccctg ccagcgagaa tccctgccag ccgaggtttg ggttgggcga
     4201 cgcggcagaa tcccggctcc gccggagtcg gatgggcaag gcgagtactt gccttaaatc
     4261 cgacctacgc ttttaacggg aaggctatcc gcgttttatt cccggagggc ggtgcatcct
     4321 cagggccgtt gaggtaatat tcgtagctga ttcccgttgg ctcatagcca tgctcaatga
     4381 tccaggcttg caatgcttcg tacggcgtgg ccatttcttc gtagggtccg gtatattcac
     4441 aggtggctac aggtcctgcc ggaatctttg agggctggac agcgcccttt cctggcaggc
     4501 tgcgggcggt ggggaatcca atctcaatat ccagagcctg catatccata ttgtagtagg
     4561 ctacaaaggg catacccacc ggatatttcc cctgggattg gatctgcgcc atgatctcgc
     4621 cataagcctg gccgagtacg gtgggcatgt cctgcaccgc gattgttgtg cggatggata
     4681 agaccggctg ctctatctgt tcgattac
//
LOCUS       DATMXK010000024         9089 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_159065, whole genome shotgun sequence.
ACCESSION   DATMXK010000024 DATMXK010000000
VERSION     DATMXK010000024.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9089)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9089)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9089
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159065"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..383
                     /locus_tag="VN363_00480"
     CDS             <1..383
                     /locus_tag="VN363_00480"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83007.1"
                     /translation="RGGNAEAAYKTAVPVSRVIFSTYVISGLMAALSGIAITMLSGSG
                     NADIGSAMTLNSITGVVIGGTAFSGGVGGVVGPIMGAITLGIIQNIISFAGITTWWET
                     LAKASIIVVALAIPGIVNLFRRRE"
     gene            380..1348
                     /locus_tag="VN363_00485"
     CDS             380..1348
                     /locus_tag="VN363_00485"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83008.1"
                     /translation="MKRFAISPPIAALILAIVLFLLSGLLPNGYGNNVSVAISQATNI
                     LRLSVFLGVIAAGQTLVIISGFEGIDLSAGAIVTMTAILTYVYVNGQNDKVLVALLIS
                     LVVGALIGFLNGVGVTFLKISPFVMTLGMSGVVTGAIIVINHGNVSGKVAPIMTRLIA
                     RPVAPNIPIPSALVIWFIFGVLMWLLLERTTFGKNLFAIGTNRVTAKLSGVNVIGMNL
                     ATYSLAGALAGFGGFLLVGNTGVVLISLGQPYLFPSIAAVAVGGTLLSGGKGSYWGTM
                     AGAILLTLITSLLTTMQMPDSVRRMVLGATLLILISIYGRQKTLRQ"
     gene            1390..2469
                     /locus_tag="VN363_00490"
     CDS             1390..2469
                     /locus_tag="VN363_00490"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWQ83009.1"
                     /translation="MTKTVKVGIVGTGFVGDIHHTSFKNWVRDAEVIAVASPHNADKF
                     AEERGIPVAYADYRQMLQDKDIDVIDIGIPNDLHYQVVVDAAKAGKHVIIEKPLCITL
                     EQADEMIETCQKAGVLLMYAEELLFAPKYVRVKTLIEEGAIGEPFLAKQSEEHPGPHM
                     PWFWDVNRSGGGVLLDMGCHSIEYTRWILGKPRVKSVTAFMGTYLHQERTQGEDHSFT
                     IVEYEGGKIAMLENSWAKGGGVDDRCEVYGKKGHTRADLLRGSSLLTYSEEGYGYAVE
                     KAGTTRGYTFTMFEEIWNYGFPQEMQHFVNCVLGKETCLETGEDGREVLKIMYAAYQS
                     AGEGRKIEWPYNPSKVSKPIDLWKK"
     gene            2504..3247
                     /gene="deoC"
                     /locus_tag="VN363_00495"
     CDS             2504..3247
                     /gene="deoC"
                     /locus_tag="VN363_00495"
                     /EC_number="4.1.2.4"
                     /inference="COORDINATES: protein motif:HMM:NF013916.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004139 - deoxyribose-phosphate aldolase
                     activity [Evidence IEA];
                     GO_process: GO:0009264 - deoxyribonucleotide catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxyribose-phosphate aldolase"
                     /protein_id="HWQ83010.1"
                     /translation="MPVLSSKTITYPQLAKVIDHSLLKPELTEQDIIEGCKLAARYNT
                     ATVCVKPCHVRLAAEVLKGSDVKVSTVVGFPHGSNLTATKVAEAHAAMDDGAVELDMV
                     LNIGQLRSGKADYVQADIQAVCEAAHARGAKVKVILENAYLNDEQKVLACRLAEAAGA
                     DWVKTSTGFAPSGATIEDLRLMRTTVSENVQVKAAGGVRTLDGLLAVIDAGVTRCGAT
                     ATAVILDEFQKRANEGTLSGGEARFGGAY"
     gene            3286..4452
                     /locus_tag="VN363_00500"
     CDS             3286..4452
                     /locus_tag="VN363_00500"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWQ83011.1"
                     /translation="MGKKLTKQTIGVGVVGTGFIGPAHIEGLRRNGIQVIGLAESTLE
                     TAQIKAAELSIPLAYASFEELLADPEIDVVHLATPNVLHYPHARAALLAGKHVVCEKP
                     LAMTAAESAELVRLAAETGKVNAINFNIRMYPMVQHARSLVQSGEIGDLFILQGSYLQ
                     DWLLFNTDWNWRLEADLGGSLRAVGDIGSHWLDLMTFITGLRVEEVYADFKTVHTTRK
                     KPGKPLETFTGKMLQPADYIDQPITTEDYATILLHYETGVRGVVTVAQVCSGRKNRLF
                     FEINGSKSSLAWDGERPNELWIGHRTEPNQVLLKDPSLLSPEARQVASYPGGHAEGFP
                     DTFKQLSKKVYDYILAGDYSQKPDFPTFADGHYEMVLCEAIEKSARQGQWVKVS"
     gene            4545..5459
                     /locus_tag="VN363_00505"
     CDS             4545..5459
                     /locus_tag="VN363_00505"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar phosphate isomerase/epimerase"
                     /protein_id="HWQ83012.1"
                     /translation="MKLGFLTAALPDNTLEQAARWGAEGGFQAIEIACWPVGKATRRY
                     AGVTHIDVSTLDKAQAREIRKMLDSYGLTISSLAYYPNPLHPDAEHRETVISHLKKVI
                     EAAALLETPVVGTFVGKDKNKTVPQNFEDFARIWPPIVRFAKDHGIKIAIENCPMIFS
                     LDEWPGGNNLASTPAIWRKMWEIIPEDNFGLNLDPSHLVLQMIDIERVVREFGPKLFH
                     VHAKDLHIDPEGLYNNGIFSQGMGWQVPRLPGLGDMDWRKFFAALTAARYDYVVSIEH
                     EDRVFEGDEELVKRGFYLSRDVLKPFIH"
     gene            5549..6529
                     /locus_tag="VN363_00510"
     CDS             5549..6529
                     /locus_tag="VN363_00510"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWQ83013.1"
                     /translation="MNYFIGCDLGGTNLRAAVVDVDTGTVINQLSISTMAREGHEAVM
                     ARMAGLFLQVIQETGLQKDAIGGIGIGVPGVLDLEKGETLFLPNLHGTWPHVPLSARI
                     SQLMDLPTAILNDARSITLGEWRFGAGKGSDTLAVFTLGTGIGGGLVVDGRLHMGKGG
                     TAGELGHMPIDYNGPRCGCGNYGCLEVFASGPAIRVMGIKAVAQGLTTQIGELCEYDL
                     NRITPKLIAQAARAGDEIAQGIYNQAGFYLGVAASSICAVIAPQRIILSGGVSRAGSL
                     LLEPMTRTMRERTHIMDVSQVEIVQGALGDNAGVLGASVWASLHTAGLPA"
     gene            6539..7573
                     /locus_tag="VN363_00515"
     CDS             6539..7573
                     /locus_tag="VN363_00515"
                     /inference="COORDINATES: protein motif:HMM:NF013539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWQ83014.1"
                     /translation="MNPYIQDILSQPETLRLALGSFSVEPLAHLHHRLHQNEFDRILI
                     TGMGASYNAAYPAYLHLANQPLPVMLVNAAELLHYSAGLIGPRSLIWLSSQSGRSIEV
                     VRLLENFHSRPPLSVLASVNDTSSPLASAANICLPIYAGAEATVSTRTYVNMLAVNLL
                     AAIALSGGDVQAAQGKLSAAAEAMQAYLDGWQTAVAELDARLGKVKNLIILGRGPSLA
                     AVWNGSLINKEAAKCTFEGMNSADFRHGPLELAAPDLTVLVFAGVQKTAGLNRELALE
                     VIRLGGRSLWIAEDPDPELPTLRLPAVAELALPLVEILPLQMLTILMAQRNGLEAGKF
                     RHVQKITLRE"
     gene            7704..8975
                     /locus_tag="VN363_00520"
     CDS             7704..8975
                     /locus_tag="VN363_00520"
                     /inference="COORDINATES: protein motif:HMM:NF015185.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ83015.1"
                     /translation="MTVVSTAPKTRLLTPLLLLFMLAMILANLGGNMYGPLEALYLQD
                     LGATVGQIGLFYTLSQIIPLILQILGGWLSDSIGRLRAIAIGSVVGIFTYVPLILATS
                     WEWVLVSSAIGAVTRSLVGPSFDAFIADHSAPENRARLFGITQTLFGVVGVVGPFLGG
                     FLVEKMDFKRMLLIAAALYLTATIIRVSMAREAASGQEANPTKLTFASLKSNLGMMVA
                     LILTGGLFTWIMITDGVRDIFFNMSFSFLSVYMQDIASLSISQIGMMNSLFGIAMMAV
                     MFPAGWLADKAGERVNIAISFLLMASTFALISLVPPASPAWIYALGWVIAGVGVGLAT
                     PAYQSLISKAVPPHLRGVAFGLFSTSLGLVSLPAPVIGGYLWENVSPQFPFMLTAIAS
                     MLSTIPVWLKFKAPDKPEQGGKPPAQSPEQS"
BASE COUNT         1996 a         2352 c         2659 g         2082 t
ORIGIN      
        1 acaggggcgg caatgctgaa gcggcttata aaactgcagt ccccgtgagc cgggtgatct
       61 tcagtaccta tgttatctcg ggtctcatgg cggctctctc cggaattgcg ataaccatgt
      121 taagcggatc ggggaacgcc gatatcggat cggccatgac attgaattcg atcaccggtg
      181 tagttatcgg aggcacagca ttcagcggtg gggttggcgg tgtggttgga ccgatcatgg
      241 gggcaattac actggggatc attcagaata tcatctcttt tgcagggatt accacctggt
      301 gggagacgct ggctaaagcc tctatcattg tggttgccct ggcaatacct gggatcgtga
      361 acttgttcag gaggagagaa tgaaacgttt tgcgatctcc cccccgattg cggcgttgat
      421 tttagcgatc gtgctattcc tgctttcagg cttattgccc aatggttacg ggaacaatgt
      481 gagtgtggcg atctcacagg cgaccaacat cctgcgtttg tctgtctttt tgggtgtgat
      541 cgccgcgggt caaaccctgg tgattatcag cggttttgag ggcatcgacc tgtcagcagg
      601 cgcgatcgtt accatgacag ccatcttaac ctatgtctat gtcaacggtc aaaatgataa
      661 ggtcctggtg gcgctcttaa tctccctggt ggtgggcgct ttgatcggat ttttgaacgg
      721 cgttggtgtc accttcctga aaatctcacc atttgtgatg acattaggaa tgtcaggcgt
      781 ggttacggga gcgattattg tcatcaatca tggcaatgtc agcggcaaag tggccccgat
      841 catgacgcgc ctgatcgccc gaccggtggc accgaatata cccatcccca gcgcgctggt
      901 gatatggttt atcttcgggg tgttgatgtg gctgctctta gagcggacga catttggtaa
      961 aaatctattt gctattggca ctaatcgggt aacggctaaa ctctccggcg tcaatgtgat
     1021 cggtatgaat ttggctacct actcattggc cggagcactg gcgggatttg ggggtttcct
     1081 tttggtaggc aatacggggg tggtattgat ttcattggga cagccctatc tattcccctc
     1141 aattgcagcg gtggcggtgg gcggcacgct tctctccggt ggtaaaggca gctactgggg
     1201 cacgatggct ggcgcaattt tgctgaccct gatcaccagc ctgctgacca ccatgcaaat
     1261 gcccgattct gtgcgcagga tggtcctggg cgccaccctg ttgatcctga tctcgatcta
     1321 cggtcgtcaa aaaactctgc gccaataacc acaaaattaa gtcaatccag agaaaatagg
     1381 aggctttcta tgaccaaaac cgtaaaagtg ggtatcgtcg ggaccggatt tgtgggcgat
     1441 attcaccaca cttcattcaa gaattgggtg agggatgcag aggttatagc tgtcgccagt
     1501 ccccacaatg cggacaaatt tgcggaagag cgcggcatcc cggtcgctta tgctgattat
     1561 cgccagatgc tgcaggacaa agacatcgac gtgattgata tcggtatccc caacgacctg
     1621 cactatcagg tagtggtgga tgcggctaaa gccggtaaac atgtcatcat cgaaaagccg
     1681 ctgtgtatta ctttagaaca ggcggatgaa atgatcgaaa cctgccagaa ggcgggcgtg
     1741 ttgttgatgt acgccgagga attgttgttc gcccccaagt atgtgcgcgt caaaaccctc
     1801 atcgaggaag gcgcaatcgg ggagcccttc ctggccaaac agtccgagga gcatcctggc
     1861 ccacacatgc cctggttctg ggatgtgaac cgctcgggcg gcggtgtgct gctcgatatg
     1921 ggctgccact cgattgaata tacgcgctgg atcctgggca aacccagggt caaaagcgtg
     1981 actgccttca tgggcactta tcttcaccag gaacgcaccc agggcgaaga tcattcattc
     2041 accatcgttg agtatgaggg cgggaagatc gccatgctgg aaaacagctg ggccaaaggc
     2101 ggcggcgtgg atgatcgctg cgaagtctat ggcaagaagg gccatacccg ggctgatctg
     2161 ctgcgcggca gctctttgct tacctacagc gaggaaggct atggttatgc ggtggagaaa
     2221 gccgggacca cccgcggcta taccttcacc atgttcgagg aaatctggaa ctacggtttc
     2281 ccccaggaga tgcagcactt cgttaactgc gtgctgggta aagagacttg cctggagacc
     2341 ggcgaagatg gccgcgaagt gttgaaaatc atgtacgccg cctaccaatc ggccggtgaa
     2401 gggcgtaaga ttgaatggcc ctacaacccg tcaaaagtca gtaaaccgat tgatttgtgg
     2461 aagaagtaaa tgatccagta tttagagaaa ttggagaaat gagatgcctg tactctcgag
     2521 taaaacgatc acttatcctc agttagccaa ggtcattgac cactccctgc tcaagccgga
     2581 actgaccgag caggatatta ttgaaggttg taagctggcc gcccggtaca acaccgcgac
     2641 ggtgtgtgtc aaaccctgcc atgtccgcct ggcggcggag gtattaaaag gcagtgatgt
     2701 gaaagtaagc acagtggtgg gtttccccca cggcagcaat ctcacggcga ccaaagtagc
     2761 tgaggcacac gccgccatgg atgatggcgc ggtggagctg gatatggtcc tgaatatcgg
     2821 ccagctgcgc tcgggcaaag cggattatgt ccaggcggat atccaggcag tctgtgaggc
     2881 cgcccatgcg cggggagcga aggtcaaggt gattcttgag aatgcttatc tgaacgatga
     2941 gcagaaggtc ctggcctgca ggctggctga agcagccggg gcggactggg tcaaaacatc
     3001 gactggtttt gctcccagtg gcgcgacgat cgaggatttg cgcttgatgc gcaccaccgt
     3061 tagtgagaat gtgcaggtca aggctgccgg cggagtgcgc acgctggatg ggttgttagc
     3121 ggtcatcgac gccggtgtca cgcgctgcgg ggcaaccgca acggcggtga tcctggatga
     3181 attccaaaag cgagccaatg aaggcacact cagtggcggt gaggcaaggt tcggcggcgc
     3241 ttattaatcc cgcccggcgc ctgccaatat aactacggag agaccatggg aaaaaaactt
     3301 accaaacaaa cgatcggggt cggcgtggtt ggaaccggtt ttattggtcc tgcgcatatc
     3361 gagggtctcc gccggaatgg gatccaggtt attgggctgg ctgaatccac cctcgaaacg
     3421 gcgcaaatta aggctgccga gctgagcatt cccctcgctt atgcctcttt tgaagagctg
     3481 ctggctgatc cggagatcga tgtggtgcat ctggctacgc ccaatgtgct ccactacccg
     3541 catgccaggg cagctctctt agccggcaag catgtcgtct gcgaaaaacc actggcgatg
     3601 acagcagccg aatcggctga gctggtcagg ctggcggcgg agacgggtaa ggtcaatgcg
     3661 attaatttta acatccgcat gtaccccatg gtgcagcacg cccgcagcct ggtgcagagc
     3721 ggcgaaatcg gcgatctgtt catcctgcag ggctcctacc tgcaggactg gctgcttttc
     3781 aataccgatt ggaactggcg cctcgaagcg gatctgggcg gaagcctgcg ggcagtgggc
     3841 gatattggct cccattggct ggatttgatg acctttatca ccgggctgcg cgtggaggaa
     3901 gtgtacgctg attttaagac ggttcacacg acccgcaaaa agccgggcaa gccactggaa
     3961 actttcaccg gaaaaatgct ccagccagca gactatatcg accagccaat caccaccgaa
     4021 gattatgcta ccatcctgct gcattacgag actggcgtgc ggggagtggt gaccgtggcg
     4081 caggtctgtt cggggcgtaa aaaccggctg ttcttcgaga tcaatggctc caaatcctct
     4141 ttagcctggg atggcgagcg tcctaacgag ctgtggatcg gtcaccggac cgagcccaat
     4201 caggttttgc tcaaggatcc ctcactgctc agcccagaag cgcgccaggt agcctcctat
     4261 cccggtggtc atgccgaagg tttcccggat acatttaaac aactgagcaa gaaagtctac
     4321 gattatattc tcgccggaga ttacagccag aagccagatt tccccacttt tgcggatggt
     4381 cattacgaaa tggtcctgtg cgaggcgatt gaaaaaagcg ccagacaggg ccagtgggtc
     4441 aaagtaagtt aatttttact tctacctgtg ctccccatac aataaggtct gccggagact
     4501 tttgggacat ttgctgcaaa cgatcaaaat gaggagataa acccatgaaa ctaggatttt
     4561 taaccgcggc actaccggac aacaccctgg aacaggctgc cagatggggc gcggaaggcg
     4621 gcttccaggc gattgagatc gcctgctggc cggtcggcaa ggctacccgc cgctacgccg
     4681 gggtcacgca tatcgatgtc agcacgctgg ataaagctca ggcacgcgag atccgcaaga
     4741 tgctggatag ctatggcctc accatctcat ctctggccta ttacccgaat ccgctccatc
     4801 cggatgcgga gcaccgcgag acggtcatca gccacctcaa aaaagtaatc gaggcggcgg
     4861 ctctgctgga aaccccggtg gttggtacgt ttgtcggaaa ggataaaaat aaaaccgttc
     4921 cccaaaactt tgaggatttt gccaggatct ggccgccgat tgtcaggttc gctaaagacc
     4981 acggaattaa gatagccatc gagaactgcc cgatgatctt ctcattggac gaatggccgg
     5041 gtggcaataa cctggcctct accccggcaa tctggcgcaa aatgtgggag atcatccccg
     5101 aggataactt tggccttaat ctggacccat cccatctggt gctgcagatg atcgatattg
     5161 aacgcgtggt gcgcgaattc ggaccaaagc tgttccatgt acatgccaaa gacctacaca
     5221 ttgaccctga aggcctgtac aataatggca tattctctca gggcatgggc tggcaggtgc
     5281 cgcgcttgcc cggactgggc gatatggact ggcgaaagtt cttcgcggcc ctcaccgccg
     5341 cccgctatga ttatgtggtc agcattgagc atgaagaccg ggtcttcgaa ggagatgagg
     5401 agcttgtcaa acgcggcttc tatctctccc gggatgtgct gaagccgttt attcattaat
     5461 ttaggcgagt tttggcagaa caataaagga tcgaatttta aacaccccct ctcctgattg
     5521 atcaagagag ggggaaaggg attaacccat gaattatttc atcggctgtg acttgggcgg
     5581 tacaaatctg cgcgcggcag ttgtggatgt ggatacgggt actgtaatca accagttatc
     5641 gatctcgacc atggcccgcg agggccacga agcagtgatg gcgcgcatgg caggcctgtt
     5701 cttgcaggtg atccaggaaa ccggcttgca aaaagacgcc atcggtggga tcggaatcgg
     5761 tgtaccgggt gtgctcgatc ttgagaaagg ggaaacgcta ttcctcccca atttgcatgg
     5821 cacctggccg catgttccct taagcgccag gatctcccag ttgatggatt tacccaccgc
     5881 cattctcaat gatgcccgct cgatcacact gggcgaatgg cgctttgggg ccgggaaggg
     5941 atcagataca ctggcggtat tcaccctggg aaccgggatt ggcggcgggc tggtagtgga
     6001 cgggcgcctc catatgggga agggcggcac ggctggtgag ttggggcata tgcccattga
     6061 ttacaatggc ccgcgctgtg ggtgcggcaa ctacggctgc ctggaggttt ttgcctcagg
     6121 cccggcgatc cgggtgatgg gaatcaaagc tgtcgcccag ggattgacca cccagattgg
     6181 cgagctgtgc gaatatgacc tgaaccggat tacccccaaa ttgatcgccc aggcagcccg
     6241 ggcgggagat gaaattgcgc agggtattta taatcaggcc gggttttatc tgggtgtagc
     6301 cgcttccagc atctgtgctg tgatcgcccc gcagcggatt atcctctcgg gtggcgtctc
     6361 gcgggctggc agcctcttgc tggagcctat gacccgcacg atgcgtgagc gtacgcatat
     6421 catggatgtg agccaggtgg aaatcgttca aggcgcgctg ggcgacaatg ccggtgtgct
     6481 tggggcatct gtgtgggcgt ccctccacac agccggcctg ccggcataga tgccgccaat
     6541 gaatccatat attcaggaca tcttatctca gccagagact ttgcgccttg cgctgggcag
     6601 cttttccgtg gaaccgcttg cacatttaca ccacaggctg caccagaacg aattcgaccg
     6661 cattctcata acggggatgg gtgcatctta taacgcggct tacccggctt acctgcacct
     6721 cgccaaccag ccgctgccag tgatgctggt caatgctgct gaactgctgc attattcggc
     6781 gggattaatc ggaccgcgca gcctgatctg gctgagctcg cagtccgggc gcagcatcga
     6841 ggtggtgcgc ctgctggaga atttccactc gcgacctccg ctcagtgttc tggcaagtgt
     6901 taacgacaca tccagcccgc tggcaagcgc tgcaaatatc tgtctgccaa tctatgccgg
     6961 agcagaagcg acggtcagta cccgcaccta tgtgaatatg ctggcagtta atcttctggc
     7021 agcgattgca ctcagcggcg gcgatgttca ggcggcacaa ggaaaattgt ctgctgcagc
     7081 ggaggcgatg caggcttacc tcgatggctg gcagactgcg gtggcggagc tggatgccag
     7141 attgggtaag gtgaaaaacc tgatcatcct ggggcgcggc ccatccctgg cagcggtctg
     7201 gaatggctcg ctcatcaata aggaagctgc taaatgtacc tttgagggga tgaattcagc
     7261 cgatttccgg catggacccc tggagctggc tgcgccggac ctgactgtgc tggtgtttgc
     7321 cggagtgcaa aaaactgccg gattgaaccg cgagctggct ctggaggtca ttcgtctggg
     7381 tggtcgcagc ctttggattg ctgaagatcc tgatccggaa ctccccacct tgcgcctgcc
     7441 cgccgtggct gagctcgccc tgcccctggt ggagattctg cctctgcaga tgctgaccat
     7501 cctgatggcg cagcggaacg gtctggaagc aggaaaattc cggcatgtcc aaaaaatcac
     7561 attaagagaa taatcagtca atttgaatgg atgttgagtt gtgaaagacc cacaatcagc
     7621 cggtgaactc tggctgcctg gtgtgtgtaa tctctatgtg attaaacctg tccagtactg
     7681 atccccttcg gaatacatac tctatgactg ttgtgtcgac cgccccgaaa acccgcttac
     7741 ttaccccgct gctcttgctc ttcatgctgg cgatgatcct cgcaaatctg gggggaaata
     7801 tgtacggtcc gctcgaagcg ctctacctcc aggacttggg cgctaccgtc gggcagatcg
     7861 ggctcttcta taccctctca cagattattc cgctgatcct gcaaatcctg ggaggctggc
     7921 tctccgattc aataggacgc ctgcgggcta ttgcgatcgg cagcgtggtg gggatcttta
     7981 cttatgtgcc gctgattctg gccaccagct gggaatgggt attggtgtct tcggcgattg
     8041 gggcggtcac acgttccctg gttggaccaa gttttgatgc ctttattgcc gatcattctg
     8101 cgccggagaa tcgcgcccgg ctgtttggga ttacccagac gctttttggg gttgtaggcg
     8161 tcgtcgggcc gttcctgggt gggtttctgg ttgagaagat ggattttaag cgcatgctgc
     8221 tgattgctgc ggcattgtac ctgacggcta cgataattcg ggtaagtatg gcccgtgaag
     8281 cagccagtgg ccaggaggcg aatcccacaa agctaacctt tgccagtttg aagtcgaacc
     8341 tgggtatgat ggtcgctttg atcctcaccg gcgggctgtt tacctggatc atgatcacag
     8401 atggagtgcg agatatattc ttcaatatgt cattcagctt cctgtcggtc tatatgcagg
     8461 atatcgccag cctgtctatc agccagattg ggatgatgaa cagtctcttt gggattgcca
     8521 tgatggcagt catgtttccc gccggctggt tggcggataa agccggcgaa agggtcaata
     8581 tcgccatctc atttctgctc atggccagca catttgcgtt gatttcgctg gtcccacctg
     8641 ccagcccggc ctggatctat gccctgggtt gggtgattgc cggggtggga gttggcctgg
     8701 cgacaccggc ttaccaatcg ttgatcagca aggctgtccc accgcatttg cggggcgtgg
     8761 cttttggttt attcagcacc agcctgggcc tggtctccct gcctgccccg gtgatcgggg
     8821 gttatttgtg ggaaaatgtc agcccgcagt tcccattcat gctcacagcg atcgcttcaa
     8881 tgttgtccac catacctgtc tggctaaaat tcaaagcgcc agacaaaccc gaacagggag
     8941 gtaaaccgcc agcccaatcg cctgagcaat cctgatctcg aagccaggct ggagcgggct
     9001 ggattcaggg gaccaaccga tgatcaaact cgacggctct gctttcaaag atgaacacgg
     9061 gcggaaaatc attttgcgcg gcgtaaacc
//
LOCUS       DATMXK010000025         4212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_170368, whole genome shotgun sequence.
ACCESSION   DATMXK010000025 DATMXK010000000
VERSION     DATMXK010000025.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_170368"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..674
                     /locus_tag="VN363_00525"
     CDS             <1..674
                     /locus_tag="VN363_00525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561467.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83016.1"
                     /translation="YRLMIAEFNEIVLLSLKVTGIALFFSTIIGIPVGVAMGLARFPG
                     RKLVVALLYTGMGFPPVVIGLFVYLLLSRSGPLGSLGLSWMAQLFTPGAMIIAQTIIS
                     FPLVAGFTMAAVMGVDPAVKQQVRALGATSRQAAMAILSEARIGVIVSIIAGFGGIIS
                     EVGAVMLVGGNIEGRTRVLTTAIVLETRKGNFDLALALGGVLLGITFIANLAMLRLQG
                     KTFDR"
     gene            671..1429
                     /locus_tag="VN363_00530"
     CDS             671..1429
                     /locus_tag="VN363_00530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561466.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HWQ83017.1"
                     /translation="MNTSHSQTPPSQNIYQIRDLVQIYENRRVLEIDSLDILSGEVLA
                     LVGPSGAGKSTLLRLLNFLEPPTQGTIRYQGVEFNPSGEMPLQKRRSVTTVFQRPMLL
                     ERTVYENVAFGLRLRGESDGSNRITAALEEVGLATLSRQRARTLSGGEAQRVALARAL
                     VLEPDVLLLDEPTANLDPYNVGLIETIACRLNKNHGTTLVLVTHNVFQAHRLANRVVF
                     LLEGKLVETANVEDFFERPQDPRTHAFVNGEMVY"
     gene            1556..2893
                     /locus_tag="VN363_00535"
     CDS             1556..2893
                     /locus_tag="VN363_00535"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWQ83018.1"
                     /translation="MSKKPTILIYLLVLLSLVLGACATPTTPAPTEPPAAAATEAPTE
                     PVATEPPAAATEEPLAPVAGDQRIILATTTSTEDSGLLEVILPDFTTQTGIEVDVIAV
                     GTGQALQLGIDGNADVLLVHARAREDEFMANGDGVRREEVMYNDFVIVGPETDPAGVK
                     GMTDAAAALAQIAAAEATFISRGDDSGTHTKEKSVWKAAGIEEPAGDWYVSAGQGMGD
                     VLNMAKEQLAYTLSDRATYLALVKEGLDLVILVEGDPVLFNPYGVIAVNPEKNDQISN
                     DLANQFIDWIISVPTQELIGQFGVADFGAPLFVPNSVLYNEAMGTAQPAESAGAALKI
                     TGMVVNEMAWTEAEVQAMATLDVEAPNSKGVMETYTGVSINDLLALAGVDPAATGVVL
                     VADDGFTAEVAIADLRACTTCILSFRSNGGFSSVMPGFDKNYGVKGVVEIQVK"
     gene            3001..3828
                     /locus_tag="VN363_00540"
     CDS             3001..3828
                     /locus_tag="VN363_00540"
                     /inference="COORDINATES: protein motif:HMM:NF012899.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83019.1"
                     /translation="MGLKYDDSGRLHIETPLMAESLMDKQLLESTETDSVFRPLPELN
                     VIKLGGQSIIDRGAAVLLPVLDEIIAARKAHQMVVLTGGGSRSRHAYAIALDLGMPTG
                     VLAKLGSSISEQNALMVSILLAPHSGVKIGQDDLVKLPSYLALGAIPVMHAMPPYGMW
                     EVNAERGRIPPHRTDVGAYLTAEVLGAKRCILVKDEAGLYTADPKKDPHAELIREIEV
                     HDLMRRDLDDLPVERSMLTALALARSVKEVFLVNGRNRGDMTRALQGENPGTRIFTR"
     gene            complement(3896..>4212)
                     /locus_tag="VN363_00545"
     CDS             complement(3896..>4212)
                     /locus_tag="VN363_00545"
                     /inference="COORDINATES: protein motif:HMM:NF014101.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF134 domain-containing protein"
                     /protein_id="HWQ83020.1"
                     /translation="MPRPRKRRRVARLPRPDALIYKPAGIPLERLSRVSLLAEELEAL
                     RLADLEGMDQVQAAERMAISRSTFQRILARARRQVALALVEGNALEVKIENGSPAHEA
                     DH"
BASE COUNT          805 a         1374 c         1153 g          880 t
ORIGIN      
        1 tttacaggct tatgatcgct gaattcaacg aaatcgtcct cctttcgctc aaagttaccg
       61 gcatcgccct ctttttcagc accattatcg gcatccctgt cggggtggca atgggtctgg
      121 ctcgctttcc aggccgcaag ctggtggtcg ccttgcttta cactggcatg ggtttccccc
      181 cggtcgtcat tggcctgttc gtatacctgc tcctctcccg ctccggtcca ctcggctccc
      241 tcggcctgag ctggatggcg cagctcttca cccccggcgc gatgatcatc gcccagacca
      301 tcatctcttt tcccctcgtt gccggcttca ccatggcggc cgtcatgggt gtggatcctg
      361 ccgtcaaaca gcaggtgcgc gccctgggcg ccacttcccg ccaggctgcc atggccatcc
      421 tgtccgaagc ccgcatcggg gtaatcgtgt cgatcatcgc cggtttcggc ggcatcatct
      481 ccgaggttgg cgcggtcatg ctcgtcggtg ggaatatcga aggccgcacc cgggtcctca
      541 ccaccgccat cgtcctcgaa acccgtaaag gcaatttcga tctggccctg gctctgggtg
      601 gggttttgct cgggatcaca tttattgcta acctggctat gctgcgtttg cagggaaaga
      661 cattcgaccg ttgaacacct cccactccca gactcccccc tcccagaata tttaccagat
      721 ccgcgatctg gtccagatct acgaaaatcg ccgtgtcctg gagatcgaca gcctcgatat
      781 cctttccggc gaagtcctgg ccctggttgg tcccagcggt gcaggaaaaa gcaccttgct
      841 gcgcctgtta aacttcctgg agccgcccac tcagggcacc atccgctacc agggagttga
      901 gttcaacccc tcgggcgaaa tgccgctgca gaaacgccgc tcagtcacga ccgtcttcca
      961 gcgcccaatg ctgctggaac ggaccgtata cgagaatgtc gcctttggtc tccgcctgcg
     1021 cggtgagagc gatggcagta accgcatcac cgcagcattg gaagaagtcg gtctcgccac
     1081 cctgtcccgc cagcgcgccc gcaccctttc tggcggtgaa gcccagcggg ttgccctggc
     1141 gcgcgccctg gttctcgagc cggatgtgct cttgctggat gaacctactg ccaatctcga
     1201 cccctacaat gtcggcctga ttgagaccat cgcctgccgc ctgaacaaaa atcacggcac
     1261 aaccctggtt ctggtcaccc acaacgtttt ccaggcccac cgtctcgcca atcgcgtcgt
     1321 gttcctgctc gaaggcaagc tggtcgagac cgccaatgtg gaagatttct tcgagcgccc
     1381 gcaagacccg cgcacccacg ccttcgtcaa tggcgaaatg gtctattaga agccagtcga
     1441 gtgaatccgg gtgtactccg gattacatcc cgacttaatg tcaggtatct tgcctgattt
     1501 cccgtccact ggccggacga ttaatccggc caacaatttt ctaggaggag attatatgtc
     1561 taaaaaaccg accattctga tttacctgct tgtcctgctg tcgctggtcc tcggcgcttg
     1621 cgccacgcca accacgccag ccccaacgga gccgccagct gctgcagcca cagaagcccc
     1681 gactgagcca gttgccaccg agccccccgc cgcggcgact gaagagccgc ttgctcccgt
     1741 cgccggggat cagcgcatca tccttgccac taccaccagc accgaagact ccggtctgct
     1801 ggaagtgatc ctgcccgact tcaccaccca gaccggcatt gaagtggatg taatcgccgt
     1861 cggcaccggc caggccctcc agctgggcat cgatggcaat gccgatgtgc ttctggtcca
     1921 cgcccgcgcc cgtgaagacg aattcatggc caatggcgat ggcgtccgcc gcgaagaagt
     1981 catgtacaac gacttcgtca ttgtcggccc cgaaaccgat cctgccggcg tcaaaggcat
     2041 gaccgacgcc gccgcggctc tcgcccagat cgccgctgcc gaagcgacct tcatctcccg
     2101 cggcgatgac tccggcaccc acaccaagga aaaatcggtc tggaaagctg ctggcatcga
     2161 agaacccgct ggcgattggt acgtttccgc cgggcagggt atgggcgatg tgctcaatat
     2221 ggccaaagag cagctggctt acaccctcag tgatcgcgcc acctacctgg ccctcgtcaa
     2281 ggaaggtctt gatctcgtga tcctggtcga aggcgatcca gtcctcttca atccctatgg
     2341 tgtcattgcc gtcaatccgg aaaagaatga tcaaatctcg aacgacctgg ccaaccagtt
     2401 catcgactgg atcatttccg ttcctaccca ggaattgatc ggtcagttcg gcgtggcaga
     2461 ttttggtgct cctctgttcg tccccaactc cgttctgtac aacgaagcca tgggcactgc
     2521 ccagcctgct gaatccgccg gtgctgctct caagatcacc ggcatggtgg ttaatgagat
     2581 ggcctggacg gaagctgaag tccaggcgat ggcgaccctg gacgtcgaag ctcccaacag
     2641 caagggtgtt atggaaactt acaccggtgt ctccatcaat gatctgctgg ctctggccgg
     2701 cgtagacccg gcagccacgg gtgtcgtcct ggttgcggat gatggcttca ctgctgaagt
     2761 cgccatcgcc gatctgagag cctgcacgac ctgcatcctg tcattccgca gcaatggcgg
     2821 ctttagctcc gtcatgcccg ggtttgataa gaactatggc gtcaaaggcg ttgttgaaat
     2881 tcaggtcaag taaagtaaaa tcaactggcc tgaagaacca ataacgcagc cgcccccggc
     2941 ggttcatttc tgaaccacac cgggggcggg ttgccatatc tttactaaga ggataaaccg
     3001 atgggactga aatacgacga cagtgggcgc ttacatatcg agacgccctt gatggctgaa
     3061 tcgctgatgg acaagcagct tttggaaagt actgagaccg attctgtctt ccgcccgctg
     3121 ccggagctga atgtgatcaa gcttggcggg cagagcatca tcgaccgcgg cgcagccgtg
     3181 ctcctgccag ttctggatga gatcatcgct gctcgcaaag cccatcagat ggtggtcctc
     3241 actggcggcg gcagccgctc ccgccatgct tacgccatcg ccctcgatct gggcatgccc
     3301 accggtgtgc tcgccaaact cggctccagc atcagcgagc agaacgcctt gatggtatct
     3361 atccttctgg cgccgcattc tggcgtgaag atcggtcagg acgacctggt gaagctgcct
     3421 tcgtatctcg ccctgggtgc gatcccggtc atgcacgcca tgccgcctta cggcatgtgg
     3481 gaggtcaacg ccgagcgcgg ccgcatcccg cctcaccgca ccgatgtcgg cgcgtacctc
     3541 accgccgaag tcctgggtgc caaacgctgc atcctggtca aagatgaagc cggcctgtat
     3601 accgccgacc ccaaaaagga tcctcacgcc gagctgatcc gcgagatcga agtccacgat
     3661 ctgatgcggc gcgatctcga cgatctgcct gtcgagcgtt ccatgctcac cgccctggcc
     3721 ctggcgcgca gtgtgaagga agtctttctg gtcaatggcc gcaatcgcgg cgacatgacc
     3781 cgtgccctcc agggagagaa tccgggcacc cgaattttta cccgttgata gtcgtctgcg
     3841 cgccatcccc ttcagaggga ccgaagggag tctgctgaaa gcaggtttag ccatctcaat
     3901 gatcagcttc gtgtgccggg ctcccgttct cgattttcac ttccagggcg tttccttcca
     3961 ccagcgccag ggctacctgg cgccgggcgc gcgccaggat gcgctgaaaa gtggagcgcg
     4021 agatcgccat tcgctcagca gcctggacct gatccatccc ttccaggtcg gccaggcgca
     4081 gcgcctccag ctcttcagcc agcaagctca cccggctcag tcgctccagc gggatcccgg
     4141 caggtttgta gatcagcgca tccggccggg gcaggcgcgc gacacgcctt cgtttccgtg
     4201 ggcggggcat at
//
LOCUS       DATMXK010000026         3339 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_67478, whole genome shotgun sequence.
ACCESSION   DATMXK010000026 DATMXK010000000
VERSION     DATMXK010000026.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3339)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3339)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3339
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67478"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..923)
                     /locus_tag="VN363_00550"
     CDS             complement(<1..923)
                     /locus_tag="VN363_00550"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83021.1"
                     /translation="MNGSPKLNNNVSPKRITAFWIVVLAVFAIYTFRLFSLQILNGET
                     YLARAEDNRSTTINLPTLRGIIYDRNGTILARNIASYNIVVTAANLPDDAGAVQKIFR
                     ELSEMISVPVNGGALSEENPYVPCRSDHGISEIADYGTNSRPYSAVEIRCDISRELAL
                     MVQEKSIDWPGISVDIQPIRDYPTGSLTASILGFLGPIPAATEEEFIAKGFLPNRDKV
                     GYAGLELEYQDILAGQNGEREVEVDVGGQILRDIKPPVNAIPGKNIRLTIDIRLQQAA
                     EAIFKKELSSWNAWLGDERYRTGVVIAMNPQT"
     gene            complement(920..1420)
                     /gene="mreD"
                     /locus_tag="VN363_00555"
     CDS             complement(920..1420)
                     /gene="mreD"
                     /locus_tag="VN363_00555"
                     /inference="COORDINATES: protein motif:HMM:TIGR03426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003674 - molecular_function [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreD"
                     /protein_id="HWQ83022.1"
                     /translation="MATLLALPILGLLLMLQTAVLSDFMLLHGTVDLILVAVSAWALQ
                     KRVDSAWQWGIIAGLLITIASGLPLGVALISYPLVVGIAMVLRQRVWQIPVLAMFVST
                     ILSTIVIQLISLVALRLIGTPIPVIESLELITLPSILLNLLAAIPVFVILSDLAEWLY
                     PEAIEV"
     gene            complement(1427..2275)
                     /gene="mreC"
                     /locus_tag="VN363_00560"
     CDS             complement(1427..2275)
                     /gene="mreC"
                     /locus_tag="VN363_00560"
                     /inference="COORDINATES: protein motif:HMM:TIGR00219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein MreC"
                     /protein_id="HWQ83023.1"
                     /translation="MIKPSTRTLQIIVLVLLVAGAILLALGGYLAPVSRYVLAPVVNA
                     QTWLATRYQAIENFFNAPQDLARLRQTNQELEAEVARLEAEIINLNQQLSETRVLSAL
                     VDFARAQPENRYLAAAVIGRDPSPFLQDLIINRGSDDGIRRGMPVVTDRGLVGRISRV
                     TANAASIQLITDPATIVNIRLEPSRAQAVLIGSITGEVTLEQIPQSAEVQPGDLLLTS
                     GLGGNYPGNILIGQITGVRSRDLDLFQSASVQPTVEFDQLEIVLIITNFNPIDISPLT
                     PAEQSP"
     gene            complement(2330..>3339)
                     /locus_tag="VN363_00565"
     CDS             complement(2330..>3339)
                     /locus_tag="VN363_00565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0000902 - cell morphogenesis [Evidence
                     IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWQ83024.1"
                     /translation="ANTLVNVRGKGITINEPSWVAINKRTRKPLAIGAEAKEMVGRTP
                     ADVIAVRPLKDGVISEFEITQAMLEYFIGKAHEQSIVPVPRPRVVIGIPSGVTEVEKR
                     AVYDAAMSAGAREVGLIEEPIAAALGAGLPVSEIRGSMIVDIGGGTSEVAIMSMGGIV
                     VSRSLRVAGDEMDQDIIQYMRNKYNLSIGERMAEQAKIAIGSAYHLPEEKTMVLRGRN
                     LITGLPEAVEVSSVEIREAIAGTTQIILDTIKDALDESPPEIIADLMDTGICLAGGGA
                     QLQALDRRLAEEVKVRVWIAEDPMSCVARGAGMILENYEGLHHLLIGLERGSTRHNQR
                     A"
BASE COUNT          768 a          910 c          891 g          770 t
ORIGIN      
        1 gtctggggat tcatagcaat taccacaccg gtgcgatagc gttcgtcacc caaccaggca
       61 ttccagctgg ataattcctt tttaaaaatt gcttctgcag cttgctgtaa gcggatatcg
      121 atcgtcaggc gaatattttt acctgggatg gcgttaaccg gaggcttgat atcccgcaat
      181 atctgtccac ctacatccac ttcaacctca cgctcaccat tctgacccgc caggatgtct
      241 tgatattcca gctccaggcc ggcataacca actttatcgc ggttaggcag aaaacctttg
      301 gcgatgaatt cttcctcggt tgccgccggg atcggaccca ggaacccgag aattgaggct
      361 gtcagtgaac ctgtaggata atcgcgaata ggttgaatat ccaccgaaat gcccggccaa
      421 tcgatcgact tttcctgtac cattaatgcc agctcgcggc tgatatcaca gcggatctct
      481 acagcagaat agggtcttga gtttgtcccg taatcggcga tttcgctgat tccgtgatct
      541 gagcggcagg gtacgtaagg attctcttcg ctcagagcgc caccgttcac cggaacagag
      601 atcatctcgg aaagctcgcg aaagattttt tgtaccgcac ccgcatcatc cggcagattt
      661 gccgcggtga ctacgatgtt atacgatgct atattccgag ccaggatcgt cccgttccga
      721 tcatagatga tcccgcgtaa ggtcggcaga ttgatggtcg tgctccggtt gtcttcggcg
      781 cgtgccaggt aagtttcccc attcaggatc tgcaaactga ataagcgaaa cgtgtaaata
      841 gcaaacacgg ctaaaaccac aatccagaac gccgtgatcc gtttagggga gacattgttg
      901 tttaatttcg gtgaaccgtt catacctcga tcgcttctgg atacagccac tcggctagat
      961 cgcttaagat tacaaataca gggattgctg ccagtaagtt taacaggata ctgggcagtg
     1021 tgatcagttc taatgattct atcaccggaa taggcgtgcc aatcaggcgc aaggcgacta
     1081 acgaaattaa ctggatcacg attgtgctca ggatcgtgct tacgaacatc gccagaactg
     1141 ggatctgcca gacccgttgg cgaagcacca tggcaattcc tactaccaac ggatagctga
     1201 tcaaggcgac acccaggggt aaaccagaag caatcgtaat cagtaagccg gcaatgatcc
     1261 cccactgcca ggctgaatca acccgttttt gtaatgccca ggcgctcact gccaccagga
     1321 taagatcgac agtgccatgc aagagcataa aatcgctcaa taccgcggtc tgcagcatta
     1381 gaagtagacc tagaattgga agagcaagca gggtcgccat attcgctcag ggagactgct
     1441 cggcgggggt caggggtgag atatcgatgg gattaaaatt tgtgatgatc aggacaattt
     1501 ctaactggtc gaattccacc gtgggttgga cagaagcact ctggaagagg tcaagatcgc
     1561 ggctgcgcac accggtgatc tgcccgatca ggatattacc cggataatta ccccccagac
     1621 cagaagtgag cagcaaatcc ccgggttgga cttcagcgga ttgagggatc tgctctaacg
     1681 taacttctcc ggtgattgaa ccaatcaata cagcctgcgc ccgcgagggc tccaggcgaa
     1741 tattcactat tgtagccggg tcggtaatga gctgaatgga ggcggcattg gcggtgaccc
     1801 gggaaattcg ccccacaaga ccgcgatcgg tgacgaccgg catcccgcgc cggattccat
     1861 cgtctgagcc gcggttgata atcaagtctt gtaggaatgg gctggggtcg cggccgatca
     1921 cagccgctgc gagataccgg ttttcaggct gggcgcgggc aaagtcaacc agggcagaga
     1981 ggacccgcgt ctcggatagc tgctgattta aattgataat ctcggcttcc aggcgagcta
     2041 cttcggcttc cagctcctga ttggtttgac gcaggcgggc taaatcttga ggtgcattaa
     2101 agaaattttc gattgcctgg tagcgggttg ccagccaggt ctgggcattc acgaccggtg
     2161 ccagaacata gcgtgaaacc ggagccagat agccacccag tgccaggaga atggccccgg
     2221 caacgagcaa caccagaacg ataatctgta aagtgcgtgt agatggtttt atcattaatt
     2281 tcagaggcgg gctgtcaccc ctgctcagct tcgccagggt gaggcagcat taagcgcgct
     2341 gattgtgccg cgtgctgccg cgttcgagtc caatcagcag gtgatgcaaa ccctcgtagt
     2401 tttccaggat catccctgcc ccgcgggcca cgcagctcat cgggtcttca gcaatccaga
     2461 cgcgcacctt aacttcctcc gccaggcgcc ggtccagggc ttgcagctga gcgccgcccc
     2521 ctgccaggca gattccggta tccatcaggt cggcgataat ctccgggggt gactcgtcca
     2581 gtgcatcctt gatcgtatcc aatatgattt gggtggttcc ggcgattgct tcccggatct
     2641 ctaccgagga aacttccact gcttccggga ggccggtgat caggttccgg ccgcgcaata
     2701 ccatggtttt ttcttcgggc aagtggtacg cagacccaat cgcaattttc gcctgttcag
     2761 ccatgcgctc gccgatcgag agattatatt tgttgcgcat gtactggatg atatcctggt
     2821 ccatttcatc gccggccacc cgcagagagc gggagacgac aattccgccc atcgacatga
     2881 tcgcgacttc tgaagtgcct ccaccgatat cgacgatcat gctgccgcgg atttcggaaa
     2941 cggggagccc ggcgcctaac gccgctgcga tgggctcttc aatcaagccc acttcccgtg
     3001 ccccagccga catagcagca tcgtagacag ctcgtttttc tacttcggtg actcctgatg
     3061 gaatcccgat tacgacccgc ggcctgggaa ccggcacaat actttgttcg tgagccttac
     3121 cgataaaata ctccagcatc gcctgggtta tttcaaattc ggagatgacg ccgtctttca
     3181 atgggcgcac cgcgatgaca tccgccggag tccggccgac catctctttt gcttctgcgc
     3241 caattgccag gggtttacgg gtgcgtttat taattgccac ccacgaaggt tcgttgatgg
     3301 tgatgccttt gccgcgtaca ttgaccagtg tgtttgcgg
//
LOCUS       DATMXK010000027         3293 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_135681, whole genome shotgun sequence.
ACCESSION   DATMXK010000027 DATMXK010000000
VERSION     DATMXK010000027.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3293)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3293)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3293
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135681"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..618)
                     /locus_tag="VN363_00570"
     CDS             complement(<1..618)
                     /locus_tag="VN363_00570"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ83025.1"
                     /translation="MLTTDKKSALYTLTHGDGAPVILLHGMAASNRHWQLLLHELALN
                     GFQGFAPDLPGHGDSPKPDDPTHYTAREIYAVFERWLEGLAIDRPVHLVGHSLGGFLS
                     LLFACNHPERTASLVLIDPFISQDQLPFTVRLINKFPGFVGRVLHRAPSWIVHLVFDL
                     DVSEARHFSSQVRYEIAGDYLRASPNFVYLGSTIWDLTPMLPQIQT"
     gene            complement(658..>3293)
                     /locus_tag="VN363_00575"
     CDS             complement(658..>3293)
                     /locus_tag="VN363_00575"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ83026.1"
                     /translation="LISASLDPENVLAQVCQSVSLMGGGERSAVFLVDPDKGQIWMAY
                     AHELSDEFVQANLSFSILQEGRTRCLTTGKPDIASDVRYAELDASFCDSLLKENILAM
                     GDFPLTTPEGQIGYLSVYHNQVHKFLPEELELLQTYASDAAIAVSNARLYARTDIALS
                     RRVRQLSILEAVGRELAANLHSDQLFTIILNYALEFTGSQWGSLGLYEPDRELINLKA
                     SRGYPHSLQTLSVHEGINGRAVLTDQILNIGDVRQEAGYQDLTGGLARAHLSVPLTYE
                     GRVLGVLTLEKPDLNAYNENDETFSASLAVQAAVAVVNAELYAEIQRRLREQSTLYLV
                     SSRLSGDRDLGSVLGTVQRAIQAALEPSGSGVYLWSEHEQNYRLQAAPISENRLQTGA
                     LPAAVPARIPGNRVNRPVRLPLTLNADSLGAYFPRLAENTPIYLKLADDLPQSLQNLG
                     QTAYQVLLVPLVVPDRRLGALLFFLDSNRIPSEDILQLVNAIASQGAISLQNALLFND
                     ISGVRDRLAAVLNSVREGILMIEMDGRISLVNESVQRITGLRVSELIGQPLASLSAEA
                     LAYLGYTRADAYFLTSTKSHSFDSDEPKATYRVGQPAAAGKPALDEHYLERVTMPVWG
                     RKHNNEGWMIVLRDISQEHLITETRQLITETLVHDLRSPLSAVVSAIDVMEGGLTNES
                     QAEIMPQALQVARRGSMRVLGMIESLLDISRLQSGSLELTRIPVDMQTVVDSVLIDFL
                     PQAQDYGVIVHSKVPPGLVASADLTKITRVITNLLDNALKFTPPGGKVAFTGASGDGL
                     IELRISDSGPGVPVDFREKIFDQFAQVPGQRGRRRGSGLGLTFCKLAVEAHGGRIWVE
                     TPSSGGSVFVFTLPAASQPSE"
BASE COUNT          697 a          923 c          992 g          681 t
ORIGIN      
        1 ggtctggatc tggggcagca tcggggtcag gtcccagatg gtgctgccca ggtagacaaa
       61 atttggcgag gcgcgcaagt aatctccagc aatctcatac cgcacctggc tgctgaaatg
      121 gcgcgcttcg cttacatcca ggtcaaaaac cagatgcacg atccaggagg gagcccggtg
      181 gagaactcgc ccgacgaaac ctggaaactt gtttatgaga cgaactgtaa acggcagctg
      241 gtcctggctg atgaaaggat cgattaaaac caggctggcg gtgcgctcgg gatggttaca
      301 ggcaaacaac aggctgagga aaccacccag ggaatggccg accaggtgta ctggccggtc
      361 tatagccagg ccctccagcc agcgctcaaa gacagcatag atttctcggg cagtgtaatg
      421 ggtgggatca tccggcttgg ggctgtcgcc atgcccgggc aaatcgggag caaagccctg
      481 gaatccattc agcgccagct cgtgcagcaa aagctgccag tgccggtttg aggcggccat
      541 cccatgcagc aggatgaccg gcgcgccatc accatgggtg agggtatata aagcggattt
      601 tttatccgtg gtcagcatgg atagatcatt ttacttggtc tttaaggaaa aataatctta
      661 ttcagaaggt tggctggctg ctggcaaggt gaagacgaac acactacccc ctgacgacgg
      721 ggtttccacc cagatccgcc cgccgtgagc ttctactgcc agtttgcaga aagtgagccc
      781 taaaccggag ccgcgccgcc ggccgcgctg cccgggtacc tgggcaaact ggtcgaatat
      841 cttctcacga aaatctactg gaacgccggg tccactgtcg ctgatgcgca gttcaatcag
      901 gccgtctccg gaagctccag taaaggcgac ctttccaccc ggtggcgtga atttcagggc
      961 gttatccagc aggttcgtga tcacccgggt aatcttggtc aaatcggcgc tggctaccaa
     1021 ccccggcggt accttgctgt gcacgatcac tccataatcc tgagcctggg gcaggaaatc
     1081 gatcaacacc gagtcaacca ccgtttgcat atcaaccgga atgcgggtga gctcaagcga
     1141 gccagactgc aggcgggaaa tatccagcag ggactcgatc atccccagga cgcgcatcga
     1201 gccgcgccgt gccacctgga gagcctgagg cataatctct gcctgggatt cattcgtcag
     1261 cccaccttcc atgacatcga tagcgctgac caccgcgctg agcggcgagc gcaggtcgtg
     1321 caccagggtc tcggtgatca gctggcgggt ttcggtgatc aggtgctcct ggctgatgtc
     1381 acgcaggacg atcatccagc cttcgttgtt gtgctttctg ccccatacgg gcatggtcac
     1441 ccgctcgagg tagtgttcat ccagggcggg ttttcctgcc gcggcaggct gtccgacccg
     1501 gtaggtggct ttgggttcat cactatcgaa ggaatggctt ttggtgctgg tgaggaaata
     1561 agcatcggcg cgagtgtagc ctaaataagc cagcgcctcg gcactcaggc ttgcgagagg
     1621 ctggccgatc agctcgctga cccgcagccc ggtgatgcgc tgcacagatt cattcaccag
     1681 cgagatgcgc ccatccattt cgatcatcag gatgccttca cgcaccgagt tcaggactgc
     1741 cgccaaccgg tcgcgcacgc cgctgatatc attgaaaagc agggcattct gaagtgagat
     1801 agctccctgg cttgcgatcg cgttgactaa ttggaggatg tcttcgctcg ggatccggtt
     1861 tgaatccagg aaaaataaca gcgcacccag gcgcctgtct gggacaacca gggggactaa
     1921 cagtacctgg taggctgtct ggcccaggtt ttggaggctt tggggcaggt cgtctgccag
     1981 tttcaggtag atgggtgtat tttctgccag tcgggggaaa taggctccca ggctatctgc
     2041 attcagggtc aacggcaggc ggacgggacg gttcacgcgg tttccgggga ttctggctgg
     2101 cacagcggct ggtagggcac cggtttgcag gcggttttct gatatcggtg cggcctgaag
     2161 ccggtaattc tgctcatgct cggaccataa ataaactccc gagccagatg gctccagggc
     2221 agcctggatt gcccgctgca ctgtccccag cacgctcccc aggtcccggt caccactcag
     2281 ccggctgctg accaggtaga gggtagactg ttctcggagg cggcgctgga tctcagcata
     2341 cagctcggca tttaccacag caactgcagc ctgcaccgcc agtgaggctg agaatgtctc
     2401 atcgttctca ttgtaggcgt tcagatcggg tttttccagg gtaagaactc ccaggacccg
     2461 tccttcgtaa gtcaaaggaa cgctcagatg ggcgcgggcc agcccgccag tcaggtcctg
     2521 gtaaccggcc tcctggcgca catcgccaat attcaggatc tgatcggtga gcacggcccg
     2581 accgttgatc ccctcgtgga ccgagagcgt ttgaaggctg tggggatagc cccggctggc
     2641 tttgaggtta attagctctc gatccggttc atataaaccc agactccccc actgtgagcc
     2701 ggtaaattcc agggcgtaat tcaagatgat ggtgaagagt tggtcggaat gcaggttggc
     2761 tgccagctcg cgtccgacgg cctccaggat cgatagctgg cggacgcgcc gcgacagggc
     2821 gatatcggtg cgggcataca ggcgggcatt tgagacggcg attgcggcgt cagaggcata
     2881 agtctggagc agctccagtt cttcagggag gaatttatgc acctggttgt gataaaccga
     2941 gagatagccg atctgccctt cgggggtggt gagcggaaaa tcgcccatcg ccaggatgtt
     3001 ttctttgagc agtgaatcgc agaagctggc atccagctcg gcgtagcgca catcgctggc
     3061 gatatcgggc tttccggtgg tcaggcatcg cgtgcgaccc tcctgaagga tcgaaaacga
     3121 cagatttgcc tgcacaaact catccgacag ctcgtgtgcg taggccatcc agatctggcc
     3181 tttatccgga tcgactaaaa agactgcact ccgctcacct ccgcccatta gactgaccga
     3241 ttggcagacc tgagccagca cattttcggg atccaggctg gcgctgatca ggc
//
LOCUS       DATMXK010000028         2255 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_21497, whole genome shotgun sequence.
ACCESSION   DATMXK010000028 DATMXK010000000
VERSION     DATMXK010000028.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2255)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2255)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2255
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_21497"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(97..1767)
                     /locus_tag="VN363_00580"
     CDS             complement(97..1767)
                     /locus_tag="VN363_00580"
                     /inference="COORDINATES: protein motif:HMM:NF017011.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83027.1"
                     /translation="MADSLPFLDRFQKRLASRQSQQLILRTAGAVSAHSQPSKAEQPV
                     LLFNASTRIQGLSLNAAFQMLTGWSLRLSGVPVHHFLCESGLQPCVLGTNREDHTKSP
                     PCKACLAQSARIYTGASTRPFQFQANQELSLAVDSLSVAELSQLQIPARQAGLSLDQE
                     IPLGSLVLPSVRWALRMHHLENSQSNRYLLRQYILSAYRVLGEFASALQSVQPAAVLV
                     FNGIMYPEAAARWVSKALGFPTYAHEVGFQRNSAFFTEGQPTAYPMDIPEDFELTEAE
                     NQRLDEYLAKRFQGQFSMAGIQFWPEMRRLDEEFLAKAAEFKQIVPVFTNVVYDTSQV
                     HANVVFPHMFAWLDHVLEIIHRHPETLFVIRAHPDEMRPNSKKLSRESVREWVKHNRL
                     DQLDNVVFIDSQEYLSSYELIQRSKFVIVYNSSIGLEATLLGVCVLCGGKARYTQVPM
                     VYFPDTPEAFDDQAEKFLSQGLNSHSVDSIPAPDEYVRNARRFLYYQLYRTSLPFGDF
                     IEASPRQGFVQLRRFNWEALLPENSPTMQIIHDGLFNHQPFILPRKSS"
     gene            complement(1782..>2255)
                     /locus_tag="VN363_00585"
     CDS             complement(1782..>2255)
                     /locus_tag="VN363_00585"
                     /inference="COORDINATES: protein motif:HMM:NF013429.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIM barrel protein"
                     /protein_id="HWQ83028.1"
                     /translation="LYDNLRRSIEALDYSGVELLLENLPPRPWYFGGQWLTNAFMDAY
                     EIRDFISSMGLNMCYDTSHHKLYCNWANVDFYAQVQVLLPYIRHLHLSDGAGLDGEGL
                     QIGEGTIDWVHFFKVLGEYHGTMIPEIWRGHQRQGEGFLIAIQRLSEAYQKAQPA"
BASE COUNT          519 a          571 c          665 g          500 t
ORIGIN      
        1 ggctgcgcgc caggcttttg agctcggtca cgagcggccg gtactgatta agcaaatcga
       61 ccgatgggat ttcaattttt tcctgatatt ttgtgctcat gaagattttc tcggcaggat
      121 gaagggctgg tggttaaata acccgtcgtg gatgatttgc atggtgggtg aattctcggg
      181 gagcagtgct tcccaattga agcggcgtaa ctggacaaaa ccctggcgag ggctggcttc
      241 gatgaagtcg ccaaaaggca gtgatgttcg gtagagctgg taatacagga agcggcgggc
      301 attccgcaca tactcatctg gagcagggat gctgtcaaca gagtggctgt tcaacccctg
      361 gctcaggaat ttctcggcct ggtcatcgaa tgcttctgga gtatccggga agtaaaccat
      421 cggaacctgg gtataacgcg cctttccgcc gcacagcaca catacaccca ggagcgttgc
      481 ttccagtccg atagatgaat tgtacacaat cacgaatttg gagcgctgga tcagctcata
      541 tgagctgagg tattcctggg agtcgataaa gacaacatta tccagttgat ctaaccggtt
      601 gtgcttgacc cactcacgga cgctctcccg gctcagtttt ttactgttgg ggcgcatctc
      661 atccgggtgc gcccggatca caaacagcgt ttcgggatgc cggtgaataa tctccagtac
      721 atggtcaagc caggcaaaca tatgtggaaa tacgacgttg gcgtgcacct ggctggtatc
      781 gtaaaccaca ttggtaaata ccggtacgat ctgtttgaat tctgcggctt ttgccaggaa
      841 ttcctcgtcg agccggcgca tctcaggcca gaattgaatt ccagccatgc tgaactggcc
      901 ttgaaagcgt tttgccaggt actcatccag gcgctggttt tcggcttcag tcagctcgaa
      961 atcctcgggg atatccatgg gataagctgt cggctggcct tcggtgaaaa aggcagagtt
     1021 gcgctgaaag ccgacttcgt gggcgtaggt tgggaatccc agggctttgg agacccagcg
     1081 cgcggctgct tcgggataca taatgccgtt gaatacaagc acggcggctg gctggaccga
     1141 ttgtaaagca gacgcgaact cgcccagtac ccggtacgct gaaaggatgt actggcgcag
     1201 taaatagcgg ttggactggg aattttccag atgatgcatg cgcagcgccc agcgcaccga
     1261 aggcagtacc aggctgccta aaggaatttc ctgatcgagg gataatccgg cctggcgggc
     1321 aggtatctgc agctggctga gctcagccac actcaggctg tcaacagcca ggctgagctc
     1381 ctgatttgcc tggaactgaa aagggcgcgt gctggcgccg gtgtagatgc gagcggactg
     1441 cgccaggcag gccttgcaag gcggagattt ggtgtgatcc tcccgattgg tgcccagtac
     1501 gcaaggctgc agaccagatt cgcacaaaaa gtgatggacg ggtacaccgg agagccgtaa
     1561 actccagccg gtgagcattt gaaatgcggc attcaggctc aaaccctgga tacgggtgga
     1621 ggcattgaac agcagaaccg gttgttcagc tttggatggc tggctgtggg cgctgaccgc
     1681 gccggctgta cgcaggatca gctgctggct ctgcctggaa gccaggcgtt tttggaaacg
     1741 atctaaaaaa ggcaggctat cagccatgtt tcccggcgtg ctcaggctgg ctgcgctttc
     1801 tggtaagctt cggacaggcg ctggattgcg atcagaaagc cttcaccctg gcgctggtgt
     1861 ccgcgccaga tttcagggat cattgtgccg tgatattcgc ccagaacctt gaagaagtga
     1921 acccagtcga tggtgccttc cccgatttgc agaccttccc catccagccc ggccccatcc
     1981 gataaatgca ggtgacggat atagggtaat aagacctgga cctgggcata gaaatcgaca
     2041 ttagcccaat tgcagtagag cttgtggtgc gaggtgtcgt agcacatatt caagcccatc
     2101 gagctgatga aatcccggat ctcataagca tccatgaaag cgttggtcag ccactgcccg
     2161 ccgaaatacc aagggcgcgg aggcaggttt tccaaaagca gctctacccc ggaataatcc
     2221 agggcttcaa tcgaacggcg cagattatcg tagag
//
LOCUS       DATMXK010000029         3803 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_79578, whole genome shotgun sequence.
ACCESSION   DATMXK010000029 DATMXK010000000
VERSION     DATMXK010000029.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3803)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3803)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3803
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79578"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..826
                     /locus_tag="VN363_00590"
     CDS             173..826
                     /locus_tag="VN363_00590"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561352.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWQ83029.1"
                     /translation="MLVGERMSHPVISVHPDVSIQDALYRMNAENIRRLPVIDRRGIL
                     VGIVTERELLHASPSDATSLSVWEVTYLLSRIKVEDIMVKNVITTTEDTPIEEAARIM
                     TDQRIGALPVVRGDRVVGIITETDLFKVFTEIMGAREPGTRITVMAANVPGTLVNISK
                     EIYAAGGDIVAMATFIGESSAERELTFKITGADKDALLEALKPYIVAVIDVREIKAE"
     gene            complement(902..1348)
                     /locus_tag="VN363_00595"
     CDS             complement(902..1348)
                     /locus_tag="VN363_00595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83030.1"
                     /translation="MSNPAQSFAQALELLVRIRSESPYRRIRITCRDQFLFQQLLDAW
                     QKHYRVVMLNFTTLFPELDGQDLPAYQSQMIPHLNRLAESKDHLVIVLENNVQSGDQV
                     VQSLLIWLVDYLPANITLVCRCSDTAPLPLGRLRVRRQLLEISLDG"
     gene            complement(1405..2214)
                     /locus_tag="VN363_00600"
     CDS             complement(1405..2214)
                     /locus_tag="VN363_00600"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83031.1"
                     /translation="MTAFATHFAFEFKTGLRSASSMLMNYLFPLGFYAMMGLVMTQVN
                     PGFRETLIPAVILVALMAAALLGMPAPLVEAREAGIFRTYKINGVPAGSILLIPMLTT
                     VFHALIAAGIISLTARPLFNGLNPQNWTALALVTLLAALNFGCLGALIAVVSNGSRAT
                     VLWSQLIFLPAMLLGGLMMPLNILPESVQGFSFLLPTTYAMQAYTGLAYRQATLIDPG
                     LAVAILVIGAFLAIGLALFLFKWDSQATRRSRLAPLALLAWLPYLAGIFLR"
     gene            complement(2211..3137)
                     /locus_tag="VN363_00605"
     CDS             complement(2211..3137)
                     /locus_tag="VN363_00605"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83032.1"
                     /translation="MQAAVEVNRFTKRYGDFTAVSEISFRVAPGEIFGLLGPNGAGKT
                     STLECLEGLRTADQGELSILGINPGREPHRLRNLIGVQLQASGLPAVMTVQEAVRFFC
                     AYHQVPPRDDLIERMGLGEKRHTQYGQLSVGQQRRLALALAVAHRPPVVFLDEPTAGL
                     DVASRVELHSLMQELKASGSAILLATHDMAEAEKLTDRVAILLRGQIVAEGTPLELTA
                     TGSGFTKISVRTEEAALQANGHAGEPSFRGVAHHQVTEEYAVYYSAEPGQTVVALMDY
                     INQQGDKLIDLRVERPTLEERFLEITHLGEPQ"
     gene            complement(3287..>3803)
                     /locus_tag="VN363_00610"
     CDS             complement(3287..>3803)
                     /locus_tag="VN363_00610"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83033.1"
                     /translation="YLAERLLAGLESQARLGRVLEALLFLAVLYERMGDGGSLNRVFE
                     RALNFARTEGYQRTLIDLGADFALIARGYSGPHLDYLKQLMDALAASIPAPEVYLGNA
                     ENLRNAGVLSQRELEVLRLVADGRTNQEIADQLFISLNTVKTHVRHIFQCLDARNRAE
                     AIARGREHSLI"
BASE COUNT          833 a         1096 c         1149 g          725 t
ORIGIN      
        1 aaatatgaat gggatattct gcaagataaa gactataacc agctgctgga aatttcccct
       61 atgcgctggc tgaactggta agatatacta tcgcttgagc cgcggcgcag ggcccggtaa
      121 atcccagacg aatgaactca ttattatttg attttgtgca ggaggtatga aaatgcttgt
      181 cggcgaacgc atgtcccacc cggttatcag cgtccacccg gatgtctcca tccaggatgc
      241 tttgtatcgc atgaatgccg agaacatccg gcgcctgccg gtcattgacc ggcgaggaat
      301 tctggtcggc atcgtcaccg agcgcgagct gctgcacgcc tcaccctccg atgccaccag
      361 cctgagcgtt tgggaagtca cctatctgct cagccggatc aaggttgaag atatcatggt
      421 gaagaatgtt atcaccacta ccgaagatac accgattgag gaagcagccc ggatcatgac
      481 ggatcagcgg ataggggcgc tgccggtggt gcggggtgac cgggtggtgg ggatcatcac
      541 cgagactgac ctgttcaagg ttttcaccga aattatgggt gctcgcgagc cgggcacccg
      601 catcaccgtg atggccgcga atgtccccgg gacgctggtg aatatctcga aagagatcta
      661 cgcggcaggt ggggatattg tcgccatggc gaccttcatc ggtgaaagca gcgccgagcg
      721 cgagctgacc tttaagatca ccggtgcgga taaagatgct ttattggaag ccctgaaacc
      781 gtatattgtg gcggtgattg acgtccggga aatcaaggcg gagtaagccg gccacccaac
      841 ctgggttacc ctttcagggt cacccatcca gggtcaccca tcctgggtta ccctttcagg
      901 gttagccatc caggctgatc tccaacagct ggcggcggac ccgcaaacgg ccgagcggca
      961 gcggtgcagt atcgctgcag cggcaaacca gggtaatatt ggcgggcagg taatcgacca
     1021 gccagattag cagcgactgg accacctgat ctccggactg gacattattc tccaggacga
     1081 ttaccaggtg gtcttttgat tctgcgagcc ggttcagatg tgggatcatc tggctctggt
     1141 aagctggcag gtcctgcccg tccagctcag gaaataaggt agtgaaattc aacataacta
     1201 cccggtagtg tttctgccag gcatccagca gctgctggaa cagaaattga tcccggcagg
     1261 taatccggat ccggcgataa gggctttctg atcttatccg gaccagaagc tcaagagcct
     1321 gcgcgaaact ctgggcagga ttgctcatcg gacgcctggc aggggcaggg ctgaatacac
     1381 ggcctctgcc agtcctatac attgctaacg cagaaaaatc cctgccaggt agggcagcca
     1441 ggccaggagc gccagcggcg ccagccggct gcgccgggta gcctggctgt cccatttgaa
     1501 caggaataga gccaggccga tcgccaggaa ggctccaatt accaggatcg ccacagccag
     1561 gcccggatcg atgagggtcg cctgccggta tgccagcccg gtgtaagcct gcatggcgta
     1621 cgtggtgggc agcagaaagg agaagccctg caccgattcg gggaggatat tcaacggcat
     1681 catcaaaccg cccagcagca tggcgggcag aaaaatgagc tgcgaccaca gcaccgtggc
     1741 tcgcgagccg ttcgagacca ccgcaatcag ggctccaagg cagccaaaat tcaaagctgc
     1801 caggagggtg accagggcca gtgcagtcca attctgcggg tttagcccat tgaagagcgg
     1861 ccgggcagtc agggagataa ttccggctgc gatcagggca tgaaagacgg tggtcagcat
     1921 gggaatgagc aggatcgaac cagccggcac gccgttgatt ttataggtgc gaaagatgcc
     1981 agcctcgcgg gcttcaacca gcggggcggg catccccagc agcgccgccg ccatcaacgc
     2041 caccaggatg accgccggga tcagcgtctc gcgaaagccc gggttcacct gggtcatgac
     2101 caggcccatc atggcataga aacccagcgg gaacaggtag ttcatcagca ttgaagatgc
     2161 gctgcgcaag ccggtcttga attcaaaggc gaagtgagtg gcaaaagcgg tcattgtggc
     2221 tctcccaggt gggtaatctc taagaagcgt tcttccaggg tggggcgctc gacccgcaaa
     2281 tcgatcaact tatcaccctg ctggttaatg tagtccatca gggctaccac tgtctgcccg
     2341 ggttcagcgg agtaatagac tgcgtattcc tcggtaacct ggtggtgggc gactccgcga
     2401 aaggagggct ccccggcgtg gccgttggct tgcaaggcag cctcttcggt gcgcacgctg
     2461 attttggtga aacctgatcc ggtcgccgtc agctcaaggg gagtgccctc ggccacgatc
     2521 tggccgcgca gcaggatggc cacccggtcg gtcagtttct cggcttccgc catatcatgg
     2581 gtcgccagca ggatggctga gccgctggct ttcagctcct gcatcaggct gtgcagctcg
     2641 acccgtgaag cgacatccag accggccgtc ggctcatcca ggaagaccac cggaggccgg
     2701 tgagcgactg ccagcgccag ggccaggcgg cgctgctggc caaccgaaag ctggccgtac
     2761 tgggtgtgac gtttctcacc cagacccatc ctttcgatga gatcatcccg cggcggcacc
     2821 tgatggtagg cgcagaaaaa gcggactgct tcctgcacgg tcatcactgc cggcaggccg
     2881 gaggcctgga gctgaactcc gatcagattg cgcagccggt gcggctcacg ccccggattg
     2941 atccccagga tcgagagctc gccctggtcc gcggtccgca ggccttccag gcattccagg
     3001 gtgctggtct tgccggcgcc gttgggccct aacagtccaa agatctcacc cggcgccacc
     3061 cggaagctga tctccgagac ggccgtaaaa tccccatagc gcttggtaaa gcggttaact
     3121 tcaactgcag cttgcatatt tccactctcc ataccaaaaa tccattgcga ttttcccagt
     3181 ataggcagaa tccactgtcc ggtcatcaca ctggaggatg atcgcagggg tgattacccg
     3241 gtggtggcgg gaatcaccct taagcaggat tggttggaga aaggggttag atcagtgagt
     3301 gttcgcgccc gcgggcaatc gcttccgccc ggttgcgggc gtccaggcat tgaaagatgt
     3361 gccggacatg ggttttgacg gtgttgaggg agataaacag ctggtcggcg atttcctggt
     3421 tcgtgcggcc atccgccacc aggcgcagca cttccagctc gcgctggctg agcaccccgg
     3481 cattgcggag attctcggca tttcccaggt aaacttccgg cgcgggaatg ctggcagcca
     3541 gggcgtccat aagctgcttc aggtaatcca gatggggccc ggaataaccc cgtgcaatca
     3601 gagcaaaatc agcacccaaa tcaatcaggg tccgctggta gccttccgtc cgggcaaaat
     3661 tcaaggcgcg ctcgaaaacc cggttgagag agccaccatc tcccatgcgc tcgtaaagaa
     3721 cagccaggaa cagcagcgct tccagcactc tgcccagcct cgcctggctc tccagcccgg
     3781 ccaggagacg ttcggccagg tac
//
LOCUS       DATMXK010000030         4384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_66964, whole genome shotgun sequence.
ACCESSION   DATMXK010000030 DATMXK010000000
VERSION     DATMXK010000030.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66964"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            206..1225
                     /locus_tag="VN363_00615"
     CDS             206..1225
                     /locus_tag="VN363_00615"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWQ83034.1"
                     /translation="MRECLLMQQLGVNGHGLVEWKQAGGDPAIAFSCTSCNLCMVACN
                     RRLSPGAVLHAWRQFPPDREGQSPAYLSLRQTDRPAHLYERYRQHYAVDEPSHQAAPG
                     RVSFFPGCAMKAFTPHQAGSAFEHLRQSLPEIGWLDGCCYDPLDKLGLVSRFETSSRT
                     LHEKVTNLKIEELITACPTCTYRLQALLPEVQVTSIYEWMAKEQTISFSGAAGLAVHD
                     ACPDRYTQRIGKAVRSLLGVTDPLMKHEGKRSLCCGAGGDVPQFNPTLAQAIAESRLA
                     EAETGGARALVTYCSTCAVQLAGYSQTLPIVHALDLLLGLEIDYRQIAERVSLLREEA
                     RETAI"
     gene            1370..2353
                     /locus_tag="VN363_00620"
                     /pseudo
     CDS             1370..2353
                     /locus_tag="VN363_00620"
                     /inference="COORDINATES: protein motif:HMM:NF018488.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="UPF0236 family protein"
     gene            2909..4119
                     /locus_tag="VN363_00625"
                     /pseudo
     CDS             2909..4119
                     /locus_tag="VN363_00625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076611114.1"
                     /note="frameshifted; internal stop; incomplete; partial in
                     the middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ISNCY family transposase"
BASE COUNT         1106 a         1059 c         1242 g          977 t
ORIGIN      
        1 cacctggacg aatccaggcc tggccgggaa gcgggtaccg cgatcatcga accggcaaaa
       61 gagaatgaac ccgccttaga agtgttattt ccacctgccc tcagacgaat agctgctgac
      121 agggctccga attgcagaga acaaacctat ggatgatcct gaaactaatc tactagaacg
      181 ttgccaccca agctgccgga gctgcgtgcg tgagtgcctg ctgatgcagc agctaggcgt
      241 caacggccac gggctggtgg agtggaagca ggccggcggc gaccccgcga ttgccttttc
      301 ctgcacctct tgcaacctgt gtatggttgc ttgcaatcgt cgattaagcc ctggcgctgt
      361 cctacatgcc tggcggcaat tcccacctga ccgtgaaggc caatcaccag cttacttgag
      421 tctgcgccag acggatagac cagctcacct atacgagcgc tatcgccaac actatgcggt
      481 ggatgagcca tcccaccagg ctgcgcccgg ccgggtctcc tttttccctg gttgtgctat
      541 gaaagccttt accccacacc aggctgggtc ggcttttgaa cacctgcgac aaagcttgcc
      601 ggagatcggc tggctggatg ggtgttgtta tgatccgttg gataaactcg gcctcgtctc
      661 tcgttttgaa acctccagcc gcactcttca tgaaaaggtt accaatctga aaatcgaaga
      721 gctcatcact gcctgcccga cctgcaccta ccggttgcaa gccctcctgc cagaggtgca
      781 ggtcacttcg atctacgaat ggatggcaaa ggagcaaacc atctcattct cgggcgcggc
      841 tggcctggcc gttcacgatg cctgcccgga ccgttacacc cagaggatcg ggaaagcggt
      901 gcgtagcctg ctgggagtga ccgacccgct gatgaaacac gagggaaagc ggtcgctgtg
      961 ctgcggggcc ggtggagatg tcccccagtt caaccccacc ctggctcagg cgatagcaga
     1021 atcccgcctg gccgaggctg agactggcgg cgcacgcgcc ctggtaacct actgcagcac
     1081 ctgcgcggtg cagttagccg gttattccca gactctcccc attgtccatg ccctggattt
     1141 acttcttggg ctggagattg actaccggca gattgctgaa cgggtttcac ttctcaggga
     1201 agaggccaga gagactgcta tatagaaata cgtgatcaag gaggcgcctg aaaaaagatc
     1261 tggaatcgaa aacagttact gtatcataaa cccatagaat acttgcaaaa agagagagtt
     1321 ccgatcaata ttgttgttga caccaatcaa caaagaaagg aactctcaga tgacagacaa
     1381 ggctgtcgaa ttaagtataa ccgtagaagt ctatggtgaa gtgattgcat acacacgccg
     1441 ggtcgcgatt aaggagtttg aaacagggat cagccagata gtgcaagaga taggcacaaa
     1501 agtactggtg gctggcttgg agggattgga cgatgaatta cgaaaaggtg tgccagcgga
     1561 atggcagaat gtcggcacag aagaacgcag catcatgagc agcttgggaa agatcagcta
     1621 ctcgaggcgc atctatagag acgaaaaggg tgtacgtcgg aaaccggtgg atgaggtctt
     1681 aggagtggag cgatatgatt gggaaagcga gcgggttcaa caaatgggag cttatctggc
     1741 ctgtgaaggg acctatcggc gagcggcgga ccaaatgagc tgtttattga aaacaaaagt
     1801 aagtcacagt aaaatccaaa agatggtttg ggaggtaggg aaccgcatcg cagatgggga
     1861 agaagcaaag aacaggaggg tattcgaaag tggggaggcc atcccaaagg ggaaggtgaa
     1921 ggctgaggtc ttatatggag aaagtgatgg cggatggttg catttgcaga gggagaaacg
     1981 gcactcagtc gaagcccggg tagccacgct ctatagtgga aagaaacctg tgggaaagaa
     2041 gcgatatcgc ttgacggata attgcagcat cgctgctctt gggcttagtg gtgatgcatg
     2101 acgggagcat gtgttgaaga cagcacatcg ttattatgat ctggaacaaa ccaacctgct
     2161 gatcacggta ggtgatggca atcaatgggt tcgacacacc ttccagcgtt ttgatatgcc
     2221 gcaggagttc gtactggatc gctttcacct gagtcgggca gctctcgcag agcctggggc
     2281 aaccgggttg cagcccaaga gacggtcaat acattgcgtc agcaaggttt cgcggccgta
     2341 tgccaggaac tgaggcaaag cattgagcaa gcttctggcc aaagaaaaga aaaactaaac
     2401 cagttctacc aatatatcta ccaccagcaa gacggtttgt tggatctgtc ccagagaggg
     2461 tattcaaggg aactgggtag cctgggtgct attgaaggaa agtagataag ctggtcatcc
     2521 atcgcatgaa gggacggggt tgctgttgga aattacgggg ggcaaggccg atgctggcgc
     2581 tttgtcagca taaagaggcc ttgaagcatc tggcttttca atacttgccg cttgagccac
     2641 cccaaaaacc tgatcgccgg aaaggattgg cattagatag aatagatcga agcgaattct
     2701 tgcaagcaca tttgccaatt tttgatggac cagatcaaga taaaccctgg gtgaaagagc
     2761 tatatcgcta tgtacactgg caatgaattc tctcttctgg agtttttatg gaaattcttg
     2821 actgtgactc gaaaacccag caaatctaaa tatgacttca aaggtatgat aagttagggg
     2881 catgaaagcc tccaagatat ttgacgagat ggtggacggg ctgcaccaca gttttgaaaa
     2941 gctgccggat taccggacag gtgcaaacac gcagtatgaa atcaaagatg cggccctgag
     3001 agcgtttggc attttcttta cccagtcggc ttcctttctg gcctatcaac gcatgttgga
     3061 agaacggaaa gggcgcagca atgtgggcag tctgtttggg gtagagcgca ttcccagcga
     3121 caaccagatc cgcaacctgt tggacccact cgaaccggaa ttgctgtacg aagtgtttca
     3181 gcaggggctg aaggcggtgg aagccagtgg gcaactggaa aactttcgca gttacgacag
     3241 gcagatactg atttcgtgtg atgacacaca gatagtttcg tcgtccaagg tgcattgtgc
     3301 caactgttcg cagcgagcgc ttgccaatga agagatccag tatgcgcact cggccatcct
     3361 gccagtggtt gtcaaagcgg gcgaaagccg ggtgtggtgt tggagctgga gtttatcagc
     3421 ccacaggacg ggcatgagaa gcaggattgt gaacgagcag gtatcaagcg ctgggtgaaa
     3481 cgcaatgctg gacgcctggc ccggggccaa tacagcctgt tgggggatga tttgttcgcc
     3541 tgtcggccta tttgcgagct gtttttggaa gcagacttta acttcatcct ggttagcaag
     3601 ccggattcgc atgtggcgct ctacgagcaa gttgacttcc tgactaaaat gggtcaggtg
     3661 gagcaagtgc gtctgcgcca ttggaacgga cgctatgggg aaatccacga atttcgtttc
     3721 gccaacgggc tgcccttgcg ccgtggagaa gacgctttgg aggtcaactg gtgcgaaatc
     3781 accgtcaccc gcgaagacaa tggccgcaag ctctaccaca atgctttcat cacccaccac
     3841 gtgctgaccc ctgataatgt cgtgccgatt gtgcgcgatg gccgggctcg ctgaaagagc
     3901 gagaacgaaa ccaacaatgt gctcaaaacc aaaggttatc acctggaaca caacctcggt
     3961 catggagatc aattcctggc caatttcctg gttactctga atctgctggc ctttctctgc
     4021 catacgctcc tggacttact cgatgaacgc tatcacctgc tgcgccaaca tctagtagtt
     4081 cgccgagatt tcttcaatga cttgcgcgcg ctgctgcgct acatgctgtt tgacaactgg
     4141 aaccacctgg tgcgtttcat gctggaaggc ttggaactca tccccgatac ctgataaatg
     4201 ccttggttta gttcgatgaa ctgcgtttca ctgcggatgt cgctcggcag ttctgtgcgc
     4261 tcgcctgagg taattaccca atctatcggg ggctttgatt tcaccagagg tgtgcttgag
     4321 acgataattt gctagctgcc ataatacgat ttttgtggcc cttggatcgc tttttcttaa
     4381 ttcc
//
LOCUS       DATMXK010000031         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_88606, whole genome shotgun sequence.
ACCESSION   DATMXK010000031 DATMXK010000000
VERSION     DATMXK010000031.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88606"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            172..1155
                     /locus_tag="VN363_00630"
     CDS             172..1155
                     /locus_tag="VN363_00630"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ83035.1"
                     /translation="MTLVSIVILTKNAAAFLPETLPAIFSQQVDFEYEVLAVDSGSQD
                     SSLELLSRYPVRVIQIPPESFNHGETRNLGARHASPESQYVVFLSHDALPANSSWLSC
                     LIEPLINDPEVAGAFSRHIPRPGANAPVVRQLVQLTQSGGSQRLVKRMPNSSAEYEAN
                     KFYYVFFSNTSSVLRKSVWEKLPFPKADFAEDAIWADKILRAGYKIVFEPDSMIMHSH
                     NYSLVEQFRQNVDHAYAMNQLFHPDFYHDNRVWFHLFAGLPVQVGKDIKFVMSDSTFK
                     QVSLVNKFVSFFVSPAWHFATMSGSWVGAHVEKMPSWLKGVVSRQERIKRT"
     gene            1152..3176
                     /locus_tag="VN363_00635"
     CDS             1152..3176
                     /locus_tag="VN363_00635"
                     /inference="COORDINATES: protein motif:HMM:NF014237.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase"
                     /protein_id="HWQ83036.1"
                     /translation="MKKLTVILSLISFLITTTYRSVQAAPETPNEPQALGIAQGAYLR
                     PATYYGDEVLKFNALVGKDLAAVMYFINWDTSGLPAGEFFNPYHPNKLFEKFGSRSPA
                     IMITWEPVSGKSPGCARNYSGRIPLNDITSGNCDTYIREFAEALQTPPMDQLRFLIRF
                     GHEMNLRESSWWPGHTGQDASAYVAAWRHVHDVFTQVNSTNAEWVWAPNYESNPRDDW
                     NDRNNYYPGDDYVDWIGVDGYNWGSPRWDTFSELYDSSAYDHVLKDFACRYPKPQLIT
                     ETGSVEGAGSKASWIADTYSRIPNFPFVRGVFWFNDFAYGDPSRADFRVTTSTTQSGN
                     VSPLPGGSNAWTNAYRNALSNSIYTSTLPSVEAATPPTTYCQTSGAQFNAAPANITLK
                     AGERSVHTITGVGYTSTQSLALKVPSGSQITGSFSPTSLQAPFGETVLTLTTSSATPP
                     GVYPVVVQGNGVDLISLQITVYQTYSISGKLLSQVSQEMSGVTIEYQSADRLIRGTVT
                     TRANNTYLIPDLPAKEYIITPQLPEYLVDPASWTVNLTASDIHDVDFTLYARPKLIID
                     HGGVVRPGSSFNITGQEFPADQVLELYINRVYHPASITTDSTGGMNISLNTGRAETGY
                     YMLTLVAGPIHASIWFQLDENGEAWRENPPFITISDETPAWDVVFLPVVR"
     gene            3234..>4189
                     /locus_tag="VN363_00640"
     CDS             3234..>4189
                     /locus_tag="VN363_00640"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ83037.1"
                     /translation="MRILIVANGYPPTAMGGVEIYTAELSRYLVSQGHQVTVFCRESD
                     FSRPDYTINTEYLDQVKIVRVVNDYKDPHSFRKTIVDPHLEQIFDEFLESEQPDLIHF
                     NHLIALSAQLPLIAATRNIPTMITLHDFWPICHRINLIDWRGQICPGPLQGVNCAACV
                     VRGNLRQKAAQAVGTTLYKLKGYLSNRKQSPDGTSLLETADPPPALASFPKIYADRLD
                     LFTRAVLACRRILVPSEYVRSQYSVNGIPFDRIDVIPLGIEPVPAAQALRPTSGNLTF
                     ATIGPLQPIKGMDLVIKAFRLVSGDHLRLKIFGRTDLFPRDHA"
BASE COUNT          979 a         1195 c         1006 g         1009 t
ORIGIN      
        1 gtggagaggt gcgctttcat ttcccaatcg acccgttagt cctgcttttc ggctatggtg
       61 gagctttatt agccgcctat tacttactga aacgcgacct cggattggct ctggtatgcg
      121 ggacgcttat cgcctcccgc ctggttacta tctgacagct gatagcagac aatgaccctg
      181 gtatccattg ttatcctcac gaaaaatgcc gcagctttcc tgcctgaaac cctgccagcg
      241 atcttcagcc agcaggtcga tttcgagtat gaggtcctgg cggtcgattc tggctcgcag
      301 gatagcagcc tggagctgct cagccggtac ccggttcggg ttatccagat tccacccgag
      361 agctttaatc acggtgagac acgcaacctc ggcgcccggc atgccagccc ggaaagccag
      421 tacgtcgtct tcctgagcca cgatgcccta cccgcaaatt ccagctggct gagctgcttg
      481 atcgagcccc tgattaacga tccggaagtc gccggtgcgt tcagccgcca cataccccgt
      541 ccgggtgcga atgccccggt cgtacgccag ctggtccagc ttacccaatc cggaggctca
      601 cagcgactgg ttaagcgaat gccgaattcc tcagcagagt atgaggccaa caaattttat
      661 tatgtgtttt tttcgaatac cagctctgtt ctgcgcaaat ccgtgtggga aaaactgcca
      721 tttcccaaag cggatttcgc tgaagatgcc atctgggccg ataaaattct gcgcgccgga
      781 tataagatcg tttttgagcc agattccatg atcatgcact cgcataacta ctcactggtt
      841 gaacaattca ggcaaaacgt cgatcacgcc tatgccatga accagctgtt ccacccggat
      901 ttttatcacg acaatcgggt ctggttccac ctgttcgccg gactccccgt gcaggttggg
      961 aaggatatca aatttgtaat gtccgattca acctttaagc aggtatcatt ggttaataag
     1021 tttgtttcat tttttgtaag tcctgcatgg cattttgcaa caatgtctgg aagttgggtt
     1081 ggggcgcacg tcgaaaaaat gccctcctgg ctcaaaggcg ttgtatcccg acaagaaagg
     1141 atcaagcgca cttgaaaaaa cttactgtta ttctgtcgct catatctttc ctgattacaa
     1201 ccacctatcg gtctgttcag gctgcacctg agacacccaa cgagccccag gccctgggta
     1261 ttgcccaggg cgcatatctg cgtccggcga catattacgg cgatgaagtc ctgaaattta
     1321 acgcactggt cggaaaagat ctggcggcgg tgatgtattt tatcaattgg gatacttcag
     1381 gcttacccgc gggggagttt ttcaaccctt accatcccaa taagctgttc gaaaaattcg
     1441 gcagcagaag tcccgcgatc atgattacct gggaaccggt gagcggaaaa tcgcctggtt
     1501 gtgcgcgcaa ttacagcggc cgcatcccat tgaatgacat cacctccggc aattgtgaca
     1561 cttacattcg cgagttcgcc gaggccttac aaacccctcc catggaccag ctgcgcttcc
     1621 tgatccggtt cggtcatgaa atgaatttac gcgaaagctc ctggtggccc ggccacaccg
     1681 gtcaggatgc cagcgcatat gtggctgcct ggcgtcatgt ccacgatgtg tttacccagg
     1741 taaattcgac caatgcagaa tgggtctggg caccaaacta cgagagcaat ccccgcgatg
     1801 attggaacga tcgcaacaat tattatccag gcgatgacta tgtcgactgg atcggggtgg
     1861 atggctacaa ttggggcagc ccacgctggg ataccttcag cgaactgtac gattccagcg
     1921 cctatgacca tgtgcttaag gatttcgcct gtcgctatcc caaacctcag ctaatcacgg
     1981 aaactggctc agttgaaggc gcaggctcca aagccagttg gatcgccgat acttacagcc
     2041 ggattcccaa ttttccattt gtccggggtg tattctggtt caatgatttc gcgtatggcg
     2101 atcccagccg cgccgatttt cgggtgacca ccagcacaac ccagtccggg aatgtttcac
     2161 ccttacccgg cggttcaaat gcctggacaa acgcctaccg gaatgcgtta tctaactcta
     2221 tctacacctc taccctgccc agtgtggaag ctgccacacc accaacgacc tattgccaga
     2281 cctccggcgc acaatttaac gctgccccag ccaatatcac ccttaaggct ggcgagcgat
     2341 ccgtccatac gatcaccggc gtaggctata ccagtaccca gagcctggca ttgaaagttc
     2401 caagtggtag ccagatcact ggcagttttt ctccgaccag cctgcaggct cctttcggag
     2461 agacagttct gaccctgaca accagcagcg caacgccgcc gggtgtttat ccagtcgttg
     2521 tccagggtaa tggggtggac ctgatctccc tccaaatcac ggtctatcaa acctattcga
     2581 tctcgggaaa gctcctgagt caggtcagcc aggaaatgag cggcgtaacc atcgaatatc
     2641 aatctgcgga tcgtctaatc cgcgggactg tgacgacccg ggcgaacaac acgtacttaa
     2701 ttcccgattt gcctgcgaaa gaatatatca tcacccctca actccctgaa tatctggttg
     2761 atcctgcctc gtggacggtt aatcttacgg cttcagatat ccacgatgta gatttcaccc
     2821 tatatgcccg ccccaaactg atcatcgatc atggtggggt cgtaaggcca ggcagctcct
     2881 tcaatattac cgggcaggaa ttccccgcag accaggttct tgagctgtac atcaatcgtg
     2941 tttatcatcc cgcctctatc accacagata gcacaggcgg aatgaatata agtctgaaca
     3001 caggcagggc cgagaccggc tactatatgt taaccctggt agcagggcct atacatgctt
     3061 cgatctggtt ccagctggac gaaaatggcg aagcctggcg tgaaaaccca cctttcatca
     3121 cgattagcga tgagacccca gcctgggatg tcgtctttct tccagttgtc cgttaacgtt
     3181 ttcgttcgcc ctcgtcaggg cacagtacaa gactgaagtc cgttccataa ccagtgagaa
     3241 ttctgatcgt cgcgaacggc tacccaccca cggcgatggg tggggtagaa atttatacgg
     3301 ctgaactatc ccgttacctg gtaagccaag gccaccaggt aacggttttt tgccgcgagt
     3361 cagatttttc ccgccccgat tatacgatca acacggagta tttagatcag gtcaagattg
     3421 tgcgcgtggt caatgattat aaagacccac attctttccg gaagacaatt gtcgatccac
     3481 atctggagca aatcttcgat gaattcctgg aatcggagca gcccgacctg atccatttta
     3541 accacctgat cgccctatct gcccagcttc cgttaattgc agccacccga aacatcccaa
     3601 caatgattac cctgcacgac ttctggccga tctgccaccg gatcaacctg atcgactgga
     3661 gaggtcagat ctgccctggc ccattacagg gtgtaaattg tgctgcttgc gttgtccgtg
     3721 gtaatttacg ccaaaaagcc gctcaggcag tcgggacaac cctctacaag ctaaaaggat
     3781 acctctccaa ccggaaacaa tccccggatg gaacaagcct gcttgagaca gcggatccac
     3841 cgccagccct cgcatccttc ccgaagattt acgccgatcg ccttgacctg ttcacccggg
     3901 cagtccttgc atgccgcagg atattggtgc cctccgaata tgtacgcagc cagtattctg
     3961 tcaatgggat cccttttgac agaatcgatg ttatcccttt agggattgag ccagtacctg
     4021 ctgctcaagc gctcagacca acctcgggaa acctcacttt tgcgacaatc ggtcctttgc
     4081 agcccattaa aggcatggat ctcgtcatta aagcgttccg gctggtgtca ggcgaccatc
     4141 tgcgtttgaa gattttcgga cgcaccgatt tatttcctcg cgatcatgc
//
LOCUS       DATMXK010000032         2757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_100769, whole genome shotgun sequence.
ACCESSION   DATMXK010000032 DATMXK010000000
VERSION     DATMXK010000032.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100769"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..537
                     /locus_tag="VN363_00645"
     CDS             <1..537
                     /locus_tag="VN363_00645"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HWQ83038.1"
                     /translation="HYNIPVNVYGLSTNALSMDLQNGYERALNAILPALAGADELSGV
                     GELAAGVTGSLAQMVVDDELTAAVRRAIRGFTVDANSLAVEVIDQAMQGSRNFLEQPH
                     TLRTLRSGELLVTRLAERGSWSEWERRGKPDLALRAQERADMLLTQHQPLPLDIDQEK
                     ELDRIYQHAVQEIAARIL"
     gene            complement(661..1821)
                     /locus_tag="VN363_00650"
     CDS             complement(661..1821)
                     /locus_tag="VN363_00650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83039.1"
                     /translation="MSTNSHNSTTALLVGTRKGGFVFTANADRSAWKPGPLLFKGWKV
                     MHMVQDQRSGRLFSAVSSDVFGPSLHTSEDGGESWQQTATAPAFTRPSASGRPVGNPD
                     EARQPEAAVQEPEKVNQIWHIRPGLESQPGVIYAGIEPAALFRSADNGQTWELNAALY
                     DHPHRPDWFPGAGGLCLHTILLDPRDAQTMYLGISTGGCYRTDDGGVSWQPRNRNVRA
                     DFLPNTLPEYGQCLHKMAMHPALPDRIYQQNHCGIYRTDDAGEFWQDIGDGRLPSRFG
                     FPIAVHPHDPETVFVALEESDQYRLSVDGRFSVWRSRDAGESWERKTKGLPEASHLVV
                     LREAMAVDQHDPAGIYIGTSTGQIFYSRDDGESWALLADFLPPVLSVEVATI"
     gene            1916..2755
                     /locus_tag="VN363_00655"
     CDS             1916..2755
                     /locus_tag="VN363_00655"
                     /inference="COORDINATES: protein motif:HMM:NF025615.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YwiC-like family protein"
                     /protein_id="HWQ83040.1"
                     /translation="MATIASRSSSTLFRRQIALPQDHGSWVFIFSPLLIGLFAGGWVS
                     TSAYLIVAAMAAFLIRQPVTMLVKIYSRRRPRRDLASAWFWSGVYVLLGLVGVAGLAF
                     SGHAYLLWLALPGVPVFIVHLLLVSRREERRQIGVELVGCGVLALAAPAAYWVGIGEA
                     DPSGWWLFGLVWFQSAASIVYAYLRLEQRELTARPPDAVLLRMSRRAFLYTGFNLFAT
                     LVLSLLNVLPDWIWLPFALQSAETLHGALVKPAIGWKPTRIGVRQLIVSTLFTLLFIL
                     VWR"
BASE COUNT          476 a          858 c          828 g          595 t
ORIGIN      
        1 cactataata ttccggtcaa tgtttacggg ctttccacca atgccctgag catggatctg
       61 cagaacgggt atgagcgggc attgaacgcc atcctgcccg ccctggccgg cgccgatgag
      121 ctttccggcg tgggtgagct ggctgccggg gtaaccggct ctctggccca gatggtggtg
      181 gacgacgagc tgaccgccgc ggtccgccgc gccatccggg gcttcactgt tgacgctaac
      241 agcctggcag ttgaagttat cgaccaggcc atgcagggca gccgcaactt cctggagcag
      301 ccccacaccc tgcgcaccct gcgctccggg gagctgctgg tcacccgcct ggcggaacgc
      361 ggcagctgga gcgaatggga gcggcgcggc aaaccagacc tggccttgcg cgcccaggag
      421 cgcgccgata tgctgctcac ccagcaccag cccctgccgc tcgacattga tcaggaaaaa
      481 gaattggacc gcatctatca gcatgcggtc caggagattg ctgcacgtat actctaatta
      541 ctaaaaacat tcctggagga ggttaattga tttgaaatca gaccccaggg gtaaatcctt
      601 cggatttcaa aacccatggg gtctgttact caagacccga atttcccctt ctcccgaaga
      661 tcagatcgtc gccacttcca ccgacaatac cggcggcagg aaatccgcca gcagcgccca
      721 gctttcgccg tcatcccggc tgtagaagat ctgcccggtg ctggttccga tatagattcc
      781 cgccggatcg tgctggtcga ccgccatggc ctcccgcagc accaccaggt gtgaagcctc
      841 gggtaagccc ttcgtcttcc gttcccagct ttcccccgca tcgcggctgc gccagacgga
      901 aaagcgcccg tccacactca ggcggtactg gtcgctctct tccagggcga caaaaacggt
      961 ttccggatca tggggatgga cagcaatggg aaagccgaag cggctgggca ggcgtccatc
     1021 gccgatgtcc tgccagaatt caccggcgtc atccgtgcgg tagatcccgc agtgattctg
     1081 ctggtagatc cggtccggga gggcaggatg catcgccatc ttatgcaggc actgcccgta
     1141 ttcgggcagg gtattgggca ggaaatcggc gcgcacattg cggttgcgcg gctgccagct
     1201 taccccgccg tcgtccgtgc ggtagcagcc gccggtcgag atgcccaggt acatcgtttg
     1261 cgcgtcacgt gggtccagca ggatggtatg caggcacagc cctccggctc cggggaacca
     1321 atccggccgg tgagggtggt cgtagagggc tgcattgagt tcccaggttt gcccgttgtc
     1381 tgcagagcgg aacaacgccg ccggctcaat cccggcgtag attacccccg gctggctttc
     1441 cagcccgggc cgaatatgcc agatctggtt gaccttctcg ggctcctgga cagccgcttc
     1501 cggctggcga gcctcatccg gattacccac tggcctgccg gaggcggatg ggcgggtaaa
     1561 ggccggggca gttgcagtct gctgccagct ctccccgccg tcctccgagg tatgcaggct
     1621 ggggccgaat acatcgctgc tcacggcgct gaacaaccgg ccgctgcgtt gatcctggac
     1681 catgtgcatc actttccagc ctttaaataa caacggtcca ggtttccagg cgctgcggtc
     1741 cgcattggcg gtaaatacaa agccaccttt gcgcgtgcca accagtaagg cggtcgtgct
     1801 gttgtgtgag tttgtactca aaattacctc catttatgtc cgaaatcggg atgctccgat
     1861 tataggacag gcgttctatg gggtcaaggg tgaattccgt ttataattgg caattatggc
     1921 tacaatcgca tccagatcca gctcaacttt atttcgccgc cagattgcac ttccccagga
     1981 ccacggctcc tgggtgttta ttttcagccc gctcctgatt ggcctctttg ctggcggctg
     2041 ggtgtctacc agtgcttacc tgatcgtggc ggcaatggct gccttcctca tccgccagcc
     2101 ggtcaccatg ctggtgaaga tctactcccg ccgccggccc aggcgcgatc tggcctcagc
     2161 ctggttctgg tcgggcgtat atgtcctgct tgggctggtt ggcgttgccg gcctggcgtt
     2221 cagcgggcat gcttacctgc tctggctggc gctgcccggt gtgccggttt tcatcgtgca
     2281 cctgctgctc gtcagccggc gtgaagagcg ccgccagatt ggggttgagc tcgtcggctg
     2341 tggcgtgctg gcactggctg ccccggcggc ctattgggtg gggatcgggg aagccgaccc
     2401 atccggctgg tggttgttcg gtctggtctg gttccaatcc gcggcttcga tcgtgtatgc
     2461 ttacctgcgc ctggagcagc gcgagctgac tgcccggcct ccggatgcag tcctgttaag
     2521 aatgagccgg cgggcgttcc tgtacaccgg cttcaatctg tttgccacgc ttgtcctgag
     2581 cctgctcaat gtcctcccgg attggatctg gctgccattt gccctacaga gcgcagaaac
     2641 cctccacggt gctctggtaa agccggcgat cggctggaag cctaccagaa tcggggtccg
     2701 ccagctgatt gtcagcaccc tgttcaccct gctgtttatc ctcgtctggc gctaaac
//
LOCUS       DATMXK010000033         5395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_79441, whole genome shotgun sequence.
ACCESSION   DATMXK010000033 DATMXK010000000
VERSION     DATMXK010000033.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79441"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1799
                     /locus_tag="VN363_00660"
     CDS             <1..1799
                     /locus_tag="VN363_00660"
                     /inference="COORDINATES: protein motif:HMM:NF012271.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWQ83041.1"
                     /translation="TMPGAPTIYYGDEVGLTGDDDPDDRRTFPWIDTGGDANYNVMRI
                     YRTLAILRKKMPALTGGDLSILLADDAANTVVYGRKVTSQAALVAINRGSSTQTISIP
                     VAGYLPDGARLNSLYWAGPKSPAFTTLVKYGQVQLTLPAMSALLLATPITDLQPPAPP
                     RNLHVTDEGSQQVSLAWDAVARNTTYNLYRSPVSGGGWVKVNPAPLSTPSFTDTGLRN
                     ARLYYYVVTALDGRGNESAYSNEVSALPHYQIGWANLQWPPTIEHTISTTTRTPNSYG
                     QVWIDGATSSPGPTDGLLAQLGFGPADSNPDGNPDWLWVDASFNVDAGNNDEFVASLL
                     PEQTGSFDYVYRYTTTNGRDWLYADLNGPIPAGALPPNPGSLTVLPSSDSVPPAIPTG
                     LHVIAASPAGVELAWDAVLDDPTLYGYEIRRSAVSGGPYTTLALLAGVTTYTDTDVLQ
                     GATYYYVVRAVDTSFNRSADSPEVQATAELRTVTLVFTVTVPATTDATGRMVYIAGTL
                     DRLDGGLPQWDPGGVVLSRVDDTHWSITLTGEEGVQIEYKYTLGSWDYVEKGAACDEL
                     GNRQLTLSFGATGTQTVNDTVLNWRNVSPCGS"
     gene            2130..3611
                     /gene="guaB"
                     /locus_tag="VN363_00665"
     CDS             2130..3611
                     /gene="guaB"
                     /locus_tag="VN363_00665"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291787.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWQ83042.1"
                     /translation="MLNDKLFTASEALTFDDVLVVPGYTEVLPADTSVLAHLGPGLDL
                     NIPILSAAMDTVTEARLAIALAREGGLGVIHRNLSPEAQAAEVDKVKRSQSGMIVEPI
                     TLPPEASVRQAEEIMSTYHISGVPVIDHSGRLVGILTNRDVRFLEAADLDDPVSKFMT
                     SQPLVTAPVGISLLDAKKLLQQHRIEKLPLVDENGYLKGLLTVKDIQKARDFPNAAHD
                     SQGRLLVAAAVGVGPDLETRAQCLVSEGIDVVVIDTAHGHSAGVIQAIQRLRRTWPDL
                     PIMAGNVVTAEGTLALINAGATAVKVGVGAGSICTTRIVAGAGMPQLSAIYNCAQAAR
                     PFGTPIIADGGIKFSGDIVKAIAAGADAVMLGSLLSGLDESPGDIVHYEGRRFKEYRG
                     MGSLGAMQGYGRDRYASGQNGSGKLVPEGIEGRIPYKGPLSDYVFQLVGGLRSGMGYA
                     GAASLDDLRNNTELVRITNAGLIESHPHDVIITREAPNYQVNS"
     gene            3617..4225
                     /locus_tag="VN363_00670"
     CDS             3617..4225
                     /locus_tag="VN363_00670"
                     /EC_number="3.2.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF002003.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003905 - alkylbase DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase"
                     /protein_id="HWQ83043.1"
                     /translation="MPAAIRLERPFFARPALQVARELLGARLVRLQDGLSISGFIVET
                     EAYRGQEDLGCHCRAGRTPRTQVMYGPPGHAYVYFTYGMHWMLNFVVEPENQPAAILI
                     RAILPDSGLDRIASRRGPQPAQHWTDGPAKLCQALSITGELNGADLCSSKAELFVETG
                     EAISDSCVTIGPRVGLYTVPEPWKSIPWRFRAQGWQPHAAAD"
     gene            4259..5068
                     /locus_tag="VN363_00675"
     CDS             4259..5068
                     /locus_tag="VN363_00675"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="enoyl-ACP reductase"
                     /protein_id="HWQ83044.1"
                     /translation="MGLLDGKTALVFGVANDRSIAWGIAQAFHAQGAAIGLSYAGEVL
                     ERRVRPLAASIGCTFIEACDVTRDEDILQVANRAQETFGQVDILVHAIAFANRDELNG
                     PYYNTSRAGFHTAMDISVYSFTALAKAFQPLLRPGGSLLTLTYYGAEKVSPHYNVMGV
                     AKAALEASVRYLAYDFGPQGVRVNAISAGPIRTLAAAGVSGFKSMHRRFTELAPLHQS
                     ITIEDVGNTAVYLSSDLAAKTTGEVIYVDSGYNILGITEPVEEPSSEKTAQ"
     gene            5096..>5395
                     /locus_tag="VN363_00680"
     CDS             5096..>5395
                     /locus_tag="VN363_00680"
                     /inference="COORDINATES: protein motif:HMM:NF016408.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polymer-forming cytoskeletal protein"
                     /protein_id="HWQ83045.1"
                     /translation="MFRKRETPPPAEEQPAPIERITSVLGSGTIWKGSLGGSGGVRIE
                     GTFEGEIALRGVLVVGETGRVTCEQLRANLVIVAGAVRGDITTQKLEIRATGRIWG"
BASE COUNT         1067 a         1713 c         1492 g         1123 t
ORIGIN      
        1 tcaccatgcc tggcgcccct acaatttact atggggatga agtcggcctt accggggatg
       61 atgatccaga cgatcgccgt accttcccct ggatcgacac cggcggcgac gcgaactaca
      121 acgtgatgcg catctaccgg accctggcaa ttctgcgcaa aaagatgcct gcccttactg
      181 gaggcgatct gagtattctc ctcgccgatg atgccgccaa tacggtcgtc tacgggcgca
      241 aggtcacttc ccaggcagcc ctggttgcca tcaaccgcgg ttcgtctact cagacgatct
      301 cgatcccggt cgctggttac ctccccgacg gcgctcgtct gaactcgctt tactgggcgg
      361 gcccgaagag tccagccttc accaccctgg tgaagtatgg tcaggtccag cttacgctgc
      421 ccgccatgag cgccctgctc ctcgcaactc cgatcaccga tctgcagccg ccggcacctc
      481 cgcgtaacct gcatgttacg gatgaaggca gccagcaggt cagcctggcc tgggatgcgg
      541 tggctcgcaa taccacctac aacctctacc gcagcccggt cagcggcggc ggctgggtaa
      601 aggttaatcc tgccccgctc agcacgccca gcttcaccga taccggcctg cgcaatgccc
      661 gcctgtatta ctatgtggtc accgccctgg atgggcgcgg caatgaaagc gcttactcga
      721 acgaggtaag cgccttaccc cattaccaga tcggctgggc aaatctacag tggcctccta
      781 caatcgagca caccattagc accaccaccc gcaccccgaa tagttacggt caggtctgga
      841 tcgatggcgc taccagctcg cctggtccga ccgatggtct gttggcgcag cttggcttcg
      901 gtcccgcgga ctccaatccg gacggtaacc cggattggct ctgggtggat gccagcttca
      961 atgtggatgc cggcaataac gacgagtttg tagccagcct gctgcccgaa cagaccggca
     1021 gcttcgatta cgtttatcgt tacaccacga ccaatgggcg cgactggtta tatgccgacc
     1081 tgaatggtcc tatccccgcc ggcgctctgc ctcccaaccc gggcagtctc accgtcctcc
     1141 ccagcagcga cagtgttccg ccggcaattc ctaccgggct gcatgtcatt gctgcttccc
     1201 ctgccggcgt ggagctggcc tgggacgctg tgctggatga ccctacctta tacggctacg
     1261 agatccgccg cagcgctgtt tccggcggcc cctacaccac cctggcgctg ctcgccggcg
     1321 tcaccactta cacagatacg gatgtcctcc agggcgcgac ctactattat gtcgtccgcg
     1381 cagtggatac ttcgttcaac cgctctgccg actcgcccga agtccaggct acagctgagc
     1441 tgcgcactgt cactcttgtc ttcacagtta cagtgcctgc caccaccgat gccaccgggc
     1501 gcatggtcta tatcgccggc accctggatc gcctggatgg cggcctgcct cagtgggatc
     1561 caggtggcgt cgttctctcc cgcgtggatg atacccactg gagcatcacg ctcactggtg
     1621 aagaaggcgt ccagatcgaa tacaaataca cactgggttc gtgggattat gtcgagaaag
     1681 gcgctgcctg cgatgagctt ggcaaccgcc agctcacttt aagctttggc gcaaccggca
     1741 cgcagacggt gaatgatacc gttctgaact ggcgcaatgt ctccccctgc gggagctgaa
     1801 cgtcaaatca gttgtttagc cctggtgcgg gagcggcaag ctcccgcacc ttctatttcc
     1861 gtggcggggt cttccccgat ttgtgccctc cgggtattgc ggagcgattt ttccaaatat
     1921 gatacaatct ttgggaatgg cactccggta aaacctcccg gaatcctgcc ttcatcctcc
     1981 cgcctcagcg ctgctcggag gattttttct aagtacctca caaccgccag ccaccatggc
     2041 tcagccaaaa tatgcgagta acaaaaactc ctgcgtgact tccatctgat aatatccagg
     2101 agcctatgcc tgaatcagga agataagcta tgctgaacga taaattgttt accgcctccg
     2161 aagcattgac atttgatgat gtgctggtcg ttcccgggta taccgaagtg ctgcctgcgg
     2221 acaccagcgt actcgctcat cttggacccg gcctcgatct caacattccc atcctttccg
     2281 ccgcaatgga caccgtcacc gaggcccgcc tggcgattgc cctggcgcgg gagggcggtt
     2341 tgggcgtcat ccaccgcaat ctgtcccccg aagcccaggc tgctgaggtc gacaaagtaa
     2401 aacgttcgca gtccggtatg atcgtagagc cgatcacact cccaccggag gcgtccgtgc
     2461 gccaggctga ggagatcatg tccacttatc atatcagcgg cgtcccggtc attgatcatt
     2521 caggccgcct ggtaggtatt ctcaccaatc gggatgtgcg cttcctggaa gcagccgacc
     2581 tggacgatcc agtctcaaaa tttatgactt cccagccgtt ggtcactgcc ccggttggca
     2641 tcagcctgtt ggatgccaaa aaacttttac agcagcaccg catcgaaaaa ttacctctgg
     2701 tcgatgaaaa cggctatctc aaaggcctgc tcaccgtcaa agatattcaa aaagcacgtg
     2761 atttccccaa cgcagcccat gattcccagg gccgcctgtt ggtcgcggcg gctgtaggtg
     2821 ttggtcccga cctggaaacc cgtgcccaat gtctggtgag cgaaggcatt gatgttgtcg
     2881 tcatcgatac tgcccacggg cattctgcgg gtgttattca ggcgatccaa cgcctgcgcc
     2941 gcacctggcc ggacctgccc attatggcgg gcaacgttgt caccgccgaa ggtaccctgg
     3001 cactgattaa tgccggagcc actgccgtga aagttggtgt gggggccgga tcgatttgca
     3061 ccacgcgcat cgtcgccggt gccggcatgc ctcagctcag cgccatttac aactgcgccc
     3121 aggcagccag accatttggc accccgatta tcgctgatgg aggcatcaag ttcagcggag
     3181 acattgtcaa agctatcgcc gccggcgctg atgccgtcat gctcggcagc ctgctttctg
     3241 ggctggatga atctccgggt gatattgtgc attacgaagg ccgccgtttt aaagagtacc
     3301 gcggcatggg cagtctgggt gccatgcagg gctacgggcg cgatcgctat gccagcgggc
     3361 aaaacggcag cggcaagctg gtcccagaag gcatcgaagg ccgcatcccg tacaaaggtc
     3421 cgttgagcga ttatgtcttc cagctggttg gtggcttacg ctcggggatg ggctatgccg
     3481 gggctgccag cctggacgac ctgcgtaata acaccgaatt ggtgcggatc accaacgccg
     3541 gcctgatcga aagccacccc cacgatgtga ttatcacccg cgaagcacca aactaccagg
     3601 tgaattcctg aatccgatgc ccgctgccat ccgcctcgaa cgtcctttct ttgctcgtcc
     3661 agccttacag gtcgcccgcg agctgcttgg ggcgcgtttg gttcgcctgc aggatggcct
     3721 cagcatttcg gggtttatcg tcgaaactga ggcctatcgc ggccaggaag acctgggctg
     3781 ccactgccgc gcgggacgga ccccccgcac ccaggtgatg tacggcccgc ccggacatgc
     3841 ctatgtttac ttcacctatg gcatgcattg gatgctcaac tttgtcgtgg aacccgaaaa
     3901 ccagcccgcg gctattctga tccgcgccat cctccccgat tcaggcctgg accggatcgc
     3961 cagccggcgc ggcccccaac cggcccagca ctggaccgat ggacctgcca agctgtgcca
     4021 ggcgctgagc atcaccgggg agctcaatgg cgccgacctg tgcagctcaa aggcggagtt
     4081 attcgtcgaa accggtgagg caatctccga ttcttgcgtg acgattggcc ctcgcgtggg
     4141 gttatatact gtgcccgagc cctggaaaag catcccctgg cgcttccggg ctcagggctg
     4201 gcagccgcac gcagctgcag actgaaccga tttcaatctt aaccattgga gaagacacat
     4261 gggactatta gacggtaaga ccgcactggt gttcggagta gccaatgacc gctcgatcgc
     4321 ctggggcatc gcccaggctt ttcacgccca gggtgctgcg attgggctga gctatgccgg
     4381 cgaagtcctc gagcgccgcg tccgcccgct ggcagcctcg attggctgca cttttatcga
     4441 ggcctgtgat gtaacccgcg atgaggatat cctccaggtt gccaaccgcg cccaggagac
     4501 tttcgggcag gttgatatcc tggtgcatgc catcgccttt gccaatcgcg acgagctgaa
     4561 cggaccctat tacaacacca gccgggctgg ttttcacacc gccatggata tcagcgttta
     4621 ttccttcacc gccctcgcca aagccttcca gcccctcctg cgccccggcg ggtcactgct
     4681 cacccttact tattacgggg cggaaaaagt ctccccccat tacaatgtca tgggcgttgc
     4741 caaagccgcc ctggaagctt ccgtccgcta cctggcgtat gattttggac cgcaaggtgt
     4801 gcgcgtcaat gcgatctccg ccggaccgat ccgcaccctg gcagctgccg gtgtatctgg
     4861 tttcaaaagt atgcaccgcc gctttaccga gcttgccccg ctccatcaga gtatcaccat
     4921 cgaagatgtt ggcaataccg ccgtttacct gtcctcagat ctggcagcga aaacaaccgg
     4981 agaggtgatt tacgtggact ccgggtacaa tatcctgggc atcaccgaac cggtggaaga
     5041 accttcctcc gaaaaaacag cccagtagcg ctcgcccggc gcttaaggat aaggaatgtt
     5101 ccgtaaacga gaaaccccgc ccccagccga agaacaaccc gctcctatcg agcgcatcac
     5161 ctccgtcctg ggttcgggga ccatctggaa aggatcgctc ggtggcagcg gcggggtgcg
     5221 aattgaaggc actttcgaag gtgagattgc cttgcgtggg gtgctggtag tcggtgagac
     5281 aggccgcgtt acctgcgagc agctgcgcgc caacctggtg atcgtggctg gcgcagtgcg
     5341 cggtgatatc accactcaaa aacttgaaat ccgtgccact ggacggatat ggggc
//
LOCUS       DATMXK010000034         2184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_79365, whole genome shotgun sequence.
ACCESSION   DATMXK010000034 DATMXK010000000
VERSION     DATMXK010000034.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2184
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79365"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..533)
                     /locus_tag="VN363_00685"
     CDS             complement(<1..533)
                     /locus_tag="VN363_00685"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83046.1"
                     /translation="MSNKIWVYIDHFKGKAVPASWEAIGAGKTLAAELGGDVTALVFG
                     QNVEDLAQQAFASGADEVLVAEDAVLADYRPEPYAVLLARLATASQPEVILFPTTVRG
                     RELAAMAAVDLKTGVMPDAVGLEVVGGAVQVTRPVYAGKLLSQARCSARPQLITLRGR
                     VFQSPAVDAERTGTPVRV"
     gene            complement(533..1303)
                     /locus_tag="VN363_00690"
     CDS             complement(533..1303)
                     /locus_tag="VN363_00690"
                     /inference="COORDINATES: protein motif:HMM:NF013200.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="electron transfer flavoprotein subunit beta/FixA
                     family protein"
                     /protein_id="HWQ83047.1"
                     /translation="MKIVVCVKQVPDSAAKVVVENGRVSWGDAPLVINPWDEFAVEAA
                     LQLKEAQGGDVIAVSVGGESAKEALKHALAMGCGEAILVSDPVLANADSGAVAQVLAA
                     AIQKMGGVDLAVFGRQAIDGDIGMTHTQTARILGWPVLGLVSSLKLEDGSVLAERTIE
                     EGRQVIKAKLPALISVSKDIGEPRYPSFMGIRKASRALIPVWTLGDLGIPAPVSVVQW
                     PAVLTPPARDVTTEMISGSSAQEIAEKLVNRILAEKVL"
     gene            complement(1382..>2184)
                     /locus_tag="VN363_00695"
     CDS             complement(1382..>2184)
                     /locus_tag="VN363_00695"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWQ83048.1"
                     /translation="ITSTARRAGEDYILNGEKTWISLATRADHMLWVGRTNPAPDDPH
                     TGLSAFLVELNSPGVRRGDMHGKLGVRAGSTGWVAFENVRVPVENRIGLEGEGFKIAM
                     SCLDNGRYTVAAGATGLIRACLEASRKYALDRQAFGREIAHFQLIQQKIAFMVQFYEA
                     ARLLYLRAGWLKNRGIRNTRETSLAKWFATDASFQAAAEAVQIHGAYGYSDEYDVERL
                     LRNSKGAMIYEGTSEIHQLMQAGYALGYREDAALRCELPAYDPDSWQA"
BASE COUNT          468 a          693 c          543 g          480 t
ORIGIN      
        1 acccggaccg gtgtgccagt tcgctccgca tcgactgctg gagactgaaa tacacgcccg
       61 cgcaaggtga tcaactgcgg acgggcgcta cagcgcgcct gcgagaggag cttgccagca
      121 tataccgggc gagtaacctg cacagctccc cccacaactt cgaggccaac tgcatccggc
      181 ataaccccgg ttttcaaatc gacagcagcc atggcagcca gctcacgccc gcggactgtg
      241 gtgggaaata agatcacctc gggctggctg gctgtggcca ggcgtgccag aagaactgca
      301 taaggttccg gtcgataatc tgccagcacc gcatcttcag ccactagaac ttcgtctgct
      361 ccactggcaa atgcttgctg ggccagatct tctacattct gtccaaatac cagtgcggtg
      421 acatcacccc ccagctcagc agccagggtt ttgcctgccc ctatggcttc ccaggaagcg
      481 ggaacggctt tgcctttgaa atgatcaatg tatacccaga tcttgttgct catcacagca
      541 ccttctccgc caggatgcgg ttaaccagtt tttcggcaat ttcctgagcg ctgctccccg
      601 agatcatttc cgtagtgaca tcccgcgccg gaggcgtcag cactgccggc cactgtacga
      661 ctgaaaccgg agccggaata cccaggtccc ccagggtcca gaccgggatt agcgcccggg
      721 aggccttgcg aatacccata aaggatgggt agcgaggctc gccaatatcc ttgctcacac
      781 tgatcagggc aggaagtttc gctttaatga cttggcgccc ttcctcgatt gtccgctcag
      841 ccaaaacgga gccatcctcg agtttcaagc tggaaacaag gcccagcaca ggccaaccca
      901 ggatacgagc agtctgagta tgagtcatcc caatatcgcc atcaattgcc tggcgcccaa
      961 agaccgccag atccacgccg cccatcttct ggatagctgc cgccaggacc tgggccacag
     1021 ccccgctgtc tgcgtttgcc agaacaggat ccgataccag gatagcctcg ccgcagccca
     1081 tcgccagggc atgtttcaga gcttccttgg cgctttcccc tcccacgctc actgcgatga
     1141 catccccgcc ctgcgcttct ttaagctgca aagctgcctc tacagcgaac tcatcccagg
     1201 gattgatcac taagggcgca tccccccagg atacccgccc gttctctact accactttcg
     1261 cggcagagtc gggcacctgt ttaacacaga ctacgatttt caagatagcc tcctttggaa
     1321 ataaatccca ttctgtgagc gtttccgttt gttttctgga ataatatccg gcacctcagc
     1381 atcaggcctg ccaggaatct ggatcgtaag cgggtaattc acaacgtaaa gcggcgtctt
     1441 ctcgatatcc caaagcatag ccggcttgca tcaattggtg aatttcgctg gttccttcgt
     1501 agatcatcgc gcccttggaa ttgcgtaata agcgctctac atcatactca tcgctgtatc
     1561 cataagcccc atggatctgg accgcttcgg cagccgcctg gaatgaggca tccgtggcga
     1621 accattttgc gagggatgtt tcgcgcgtgt tccgtatgcc cctgttcttc agccagccgg
     1681 cccgcaggta taacagccgg gctgcctcat aaaattgtac catgaaggct atcttttgtt
     1741 gaattagctg aaagtgcgca atttcacggc cgaacgcctg acgatcgagt gcatattttc
     1801 ggctggcttc caggcaggcg cggatcaacc ctgtcgcccc tgccgcgacc gtgtaccgtc
     1861 cattatccag gcaggacatg gctatcttga acccttcacc ttccagccca atccggttct
     1921 caacgggcac acgcacattt tcaaatgcga cccagccagt ggatccagcc cgcactccca
     1981 gcttgccgtg catatcgccg cgccttaccc caggcgaatt taactcgacc agaaaggcag
     2041 ataacccggt atggggatcg tccggtgctg gattggtccg gcctacccag agcatgtgat
     2101 ctgccctggt tgccagtgag atccatgtct tttcgccatt gagtatgtaa tcttcgccag
     2161 cccggcgcgc agtacttgta atcc
//
LOCUS       DATMXK010000035         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_8703, whole genome shotgun sequence.
ACCESSION   DATMXK010000035 DATMXK010000000
VERSION     DATMXK010000035.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8703"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2013
                     /locus_tag="VN363_00700"
     CDS             <1..>2013
                     /locus_tag="VN363_00700"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWQ83049.1"
                     /translation="LTALTTLAAYHIPGATANVIPSIITVPGRGLMTRTLPRREFLKA
                     TRTLRRSQQISPEEIARVLVGLGYEPVNTVIGPGQFARRGGILDIWPAADAYPSRLDF
                     FGDEIDTLRRFDPATQITFKHSHPAQAEIAAPERVLLTPAREYLVPLERQGGENSSEI
                     LEYSEFYLPVLHSSPASLLDYLPRNTLVVIDNLVALGDLVQEIEEQAVSLRRDSIQEG
                     TLPPDFPVPYLSWSELQDSLNRYQVLELGMGEIFDPLEEDAHSPGELFLPGPRFGGRL
                     KPLIDHMVERYQDGEIQVVVSRQSARLRELWNETHPAPVEDGTQPEFIENSLGEGWIF
                     NTPGGRVINLLTDGEIFGWRRPEPRRRHRPVASSPEAAYSDLQPGDWVVHADHGIGHF
                     EGLVRRTVEGIEREYLCIEYADQAQLFVPVHQVDRLTRYVGADNRTPTPTRLGGTEWR
                     SVKAHVKEAVQEVAEELLDLYATRNVVEGHAFSPDTAWQKELETSFPYIETDDQVRVL
                     GEVKRDMESMRPMDRLICGDVGYGKTEVALRAAFKAVMDGKQVAMLVPTTVLAQQHFN
                     TFLQRLAAFPVTVEMLSRFRTPQEQRRILKRLQTGGIDILIGTHRLISSDVNFKDLGL
                     LIIDEEQRFGVTHKESLKKLRTEIDVLTLTATPIPRTLYMALSGVR"
BASE COUNT          439 a          576 c          572 g          426 t
ORIGIN      
        1 cctgacggca ttgacaaccc tggcagccta tcatatccct ggcgcgaccg ccaacgtgat
       61 cccatcaatc ataacggttc caggacgggg gttgatgacg cgcacactgc cgcggcgaga
      121 atttctaaaa gcgacgcgca ctttacgccg cagtcagcaa atctcacccg aggaaattgc
      181 ccgggtattg gttggcctcg gctacgagcc agtcaataca gtgatcgggc ctggtcaatt
      241 cgcccggcga ggcggtattc tggatatctg gccggctgcc gacgcctatc cctcccgctt
      301 ggatttcttc ggagatgaga tcgacacctt acggcgcttc gacccggcga cccagattac
      361 cttcaagcat agccaccctg ctcaggctga gattgcggct ccagagcggg tactgctcac
      421 cccggcccgc gaatatttag ttccactgga gagacaggga ggggagaata gcagcgaaat
      481 cctggaatac agcgaatttt accttccagt gctgcattcc tccccggcca gtttgctgga
      541 ctatctgccg cgcaacacat tggttgtgat tgacaattta gtagcgctcg gggatcttgt
      601 tcaggaaatc gaggaacagg ctgtgagcct gcgccgcgat tctatccagg agggcacact
      661 gccgcccgat ttcccggtcc cttatctgag ctggtccgag ctgcaggatt ccctaaaccg
      721 ctatcaagta ctcgaactgg gtatgggtga gattttcgat ccgctcgagg aagacgccca
      781 ctcgcctggc gagctatttc tgccaggacc acgttttggc gggcgtttga agcccctcat
      841 agatcatatg gtcgagcgtt accaggatgg cgaaattcag gtcgtcgtct ctcgccaatc
      901 cgctcggctc cgcgaactgt ggaatgaaac ccatcctgcc ccagttgaag acggcaccca
      961 accagagttt atcgaaaaca gtctgggtga aggctggatc ttcaatacgc ccggggggcg
     1021 ggtaatcaat ctcttaaccg atggtgaaat tttcggctgg cgccgcccag agccccgccg
     1081 ccgtcaccgg ccggtggcaa gctcacccga agctgcttac tccgacttgc agccaggaga
     1141 ttgggtcgtc cacgctgatc acggcattgg gcattttgaa ggtctggtgc gccgtacggt
     1201 tgaaggaatc gaacgcgaat acctgtgcat cgagtacgca gaccaggccc agttgtttgt
     1261 cccggtccac caggtagacc ggcttacccg ctatgtggga gcggataacc gtacgccaac
     1321 tcctacccgc ctgggaggca cagaatggcg cagcgttaaa gcccacgtca aggaagccgt
     1381 ccaggaggtt gctgaggagc tgcttgattt atacgccacc cgcaatgtgg ttgaaggcca
     1441 tgctttcagc ccggatactg cctggcagaa agagctggaa accagtttcc cttacatcga
     1501 aactgatgat caggtgcgcg tgctggggga agtgaaacgc gatatggaaa gcatgcgacc
     1561 gatggatcgc ctgatctgtg gggatgtggg ctatggcaag acggaggttg cgctgcgggc
     1621 ggctttcaaa gccgtgatgg atggcaagca ggtagccatg ctggtgccta cgacagtgct
     1681 cgcccaacag catttcaaca ccttcctgca gcgcctggca gcctttccgg tcacagtcga
     1741 gatgctctct cgctttcgga ccccacagga gcagcgccgg atcctgaagc gcttacagac
     1801 cggtggaatc gatattttga tcggcaccca ccgcctgatt tcttccgatg taaattttaa
     1861 agacttaggg ctgctgatca ttgatgaaga gcagcgcttt ggcgtgacgc acaaagaaag
     1921 ccttaagaag ctgcgaacgg aaatagacgt cctgactctg accgcaacgc caattcctcg
     1981 cacgctgtat atggctctca gcggcgtccg cga
//
LOCUS       DATMXK010000036         2224 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_79093, whole genome shotgun sequence.
ACCESSION   DATMXK010000036 DATMXK010000000
VERSION     DATMXK010000036.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2224)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2224)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2224
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79093"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..722
                     /locus_tag="VN363_00705"
     CDS             <1..722
                     /locus_tag="VN363_00705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83050.1"
                     /translation="LGECDHRAALYMQIDGYPENLEEFNAVSVRHGADPLPRLLIVLD
                     EFSALMTTLGGPKSSFANRVAELGWRGRKFGIHLVFAAQDFTKQVVGRVRDQVSAVVC
                     FRVRSLEAARAMGCPEAVRIPESRPGLACTDRWGLVQTFYLDKCMFTQSDTKAVLNHQ
                     ELQLAQRALNEANGKMSIPLLVSWGLPERGARSLVESWELRGWLRQDSARQNARYITP
                     KLLEILSNSQSGQTASSTSNW"
     gene            734..1507
                     /locus_tag="VN363_00710"
     CDS             734..1507
                     /locus_tag="VN363_00710"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWQ83051.1"
                     /translation="MPTTMAILNQKGGVGKTTTAVTLASGLARNGYHVLLIDLDTQGN
                     VADSLGLPHHDDLRRLLSPDLRFPLEQVITSSGIDHLDVIRSDKSTTALKQTLAGVTL
                     REYILADVLASAVHDVILLDCAPSADLLHFAALVAADYLLIPTRLDKLAVNGVRDALQ
                     TLAALKRISRCQVAGILPTFYERVTLESHEQLIHLAQTFGRLVLPPIPQDTQCRVATR
                     YGKTLWDYAPNAKALNGYEQGNRRVGGYVQVLERVKEWL"
     gene            1504..1911
                     /locus_tag="VN363_00715"
     CDS             1504..1911
                     /locus_tag="VN363_00715"
                     /inference="COORDINATES: protein motif:HMM:NF019493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83052.1"
                     /translation="MKNDTSKGMTSFTDLSAVDPAVESLLGQGQRRQDEARLPRTERS
                     RKKKERERAEKRKPNRINLDLPVELKKRLELLAKKEGVPISQLVAFLLYEPVYQLESR
                     AISLWGYKTASGCPKFECNLDLKRRADEAARKS"
BASE COUNT          547 a          559 c          638 g          480 t
ORIGIN      
        1 ctctgggaga atgcgaccac cgggcggctt tatatatgca aatagatggc tacccagaaa
       61 atctcgagga gttcaatgct gttagcgtcc gtcatggggc agacccgctt cctcggctcc
      121 taatcgtgtt ggatgaattc agtgccctga tgacaaccct gggcgggcct aaaagcagct
      181 tcgctaatcg ggtcgcagag ttgggttggc gtgggcgaaa gttcggaatt caccttgtat
      241 ttgccgccca agatttcacc aagcaggtag ttgggcgggt tcgtgatcag gtgagtgctg
      301 tagtgtgctt ccgtgtgcgt agccttgaag ccgcccgggc aatgggctgc cctgaagcgg
      361 tgcgcattcc agagagtcgc cctggtctgg cctgcaccga ccgttgggga ctggtgcaaa
      421 ctttctacct ggataagtgc atgtttaccc aaagtgacac taaagccgtt ctcaaccatc
      481 aggaactgca attggcgcaa cgagctttga acgaagcaaa tggcaagatg tctatcccgt
      541 tgctggtttc gtgggggctg ccagagcgcg gggcgcgttc gctggttgag agctgggagt
      601 tacgcggctg gctgcgccag gattcagctc gtcaaaatgc tcgttatatc accccgaaat
      661 tgctggaaat cctgtcaaac agtcaaagcg gtcaaactgc gtcaagcaca tcaaactggt
      721 gaggagtaag aatatgccta cgactatggc aattttgaac caaaaaggcg gtgtaggtaa
      781 aacaaccacg gcggttactc tggcttctgg attagccaga aatggctacc atgttctact
      841 gatcgacctg gatacacaag gcaatgtggc tgacagcctg ggtttgccgc atcatgacga
      901 tctgcgccgg ctgctttcac ccgatctgcg cttcccactt gaacaggtga tcacctcaag
      961 cgggatagat catctggatg taatccggtc tgataagagc acaaccgcct taaaacaaac
     1021 gctggctggt gtgaccctac gggagtatat cctggccgat gttttggcaa gcgcggttca
     1081 tgacgtgatc ctgctggact gcgccccgtc agctgatctg ctacactttg ccgcgctggt
     1141 cgctgctgat tatctactca tccccactcg gcttgacaag ctcgcggtaa atggtgtgcg
     1201 cgatgccctg caaacgttgg ctgcattgaa gcgcatcagc cgttgccagg tggctggcat
     1261 cctgccgact ttctacgaac gggttacttt ggaaagccac gaacagctga tccacttggc
     1321 gcaaacattt ggccgtttgg tgctcccacc tatcccgcaa gatacccaat gccgagtggc
     1381 aactcgctac gggaagacgc tgtgggacta cgctccaaac gccaaagcgc tgaatggtta
     1441 tgagcaaggc aaccggcgag tgggcggata tgtacaagtt ttggagcgag taaaggagtg
     1501 gctatgaaaa acgatacatc gaaagggatg accagcttca cggatctatc agctgtagat
     1561 ccagcagtag agtctctgct agggcaggga cagcgccgcc aggatgaagc ccgtttacct
     1621 cgaaccgagc gatctcgtaa gaagaaagag cgtgagcgcg ccgagaagcg taaacccaac
     1681 cgcatcaatc tcgacctccc agtggaacta aaaaaacgac tggagctcct ggcaaagaag
     1741 gagggtgtgc ccatcagtca actggtagct ttcttactct acgaaccggt ctaccagctg
     1801 gagagcaggg cgatcagcct gtggggttat aagaccgcct ccggctgccc caagttcgag
     1861 tgcaacctgg acctgaaacg ccgggcagat gaagcggcgc ggaaatcgtg aaaagttggc
     1921 tgtgtagggc gtcctaaggc gatttgtagg gcgtcccact agggcatcct attagggcgt
     1981 cctattggag aaaacccgaa aaatcatagg gtgtcctatg agacaacagg aaggcccagg
     2041 agctgcgcaa tataaggggt ggctatcttt agtcatgaac ctaagaaacg cccttagaag
     2101 ggctaaaaac ctaagacaag aatggagatc gaaaaatgac ggacacgctg catctggacg
     2161 aattccaggc atatttgttg gccgaggatc gctcgccggt aacgataata ggatatttgg
     2221 gtga
//
LOCUS       DATMXK010000037         7210 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_20561, whole genome shotgun sequence.
ACCESSION   DATMXK010000037 DATMXK010000000
VERSION     DATMXK010000037.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7210)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7210)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7210
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20561"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1263
                     /locus_tag="VN363_00720"
     CDS             <1..1263
                     /locus_tag="VN363_00720"
                     /inference="COORDINATES: protein motif:HMM:NF028259.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83053.1"
                     /translation="LYYPLLVIMLVALALAAAGLGALLVMGVVRIFPARRVAEVLGFI
                     GAISSFICSQSGQLANFEDVPPDQAMQMMANISRFSSPWSPLSWPGEALVRLGSGQWL
                     VGLGFLVLTLGLCGLLFFSSLGVAERLYYSGWANMQNNRRKRKPTSTAATAQAQPSPL
                     SGLLSQPLRAILVKDYFMLRRELRNMSQLVTPLIFGIVYAVMLLRGDDPLPAPDSETP
                     TVLAEAMQNMPLYLSVALAMFVSWMLVSRLAGMGFSQEGKNYWLLKTAPLTSKQLLLA
                     KFLVAFLPSLILGWIFLLIISMLRPGGLSQLWFTLLVVALTTAANTGINLAFGVAGAN
                     MTWEDPRKMTTGMNGCLASLASLIFLPVCLGLFFSPPILIPLVVDTPPFVGQLVGLLL
                     GGIVCLAATVLPPRLVQARVPVLDEPAL"
     gene            complement(1349..4093)
                     /gene="ppdK"
                     /locus_tag="VN363_00725"
     CDS             complement(1349..4093)
                     /gene="ppdK"
                     /locus_tag="VN363_00725"
                     /EC_number="2.7.9.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559833.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050242 - pyruvate, phosphate dikinase
                     activity [Evidence IEA];
                     GO_process: GO:0015976 - carbon utilization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate, phosphate dikinase"
                     /protein_id="HWQ83054.1"
                     /translation="MDKKWVFLFEEVEEAEKEVGGKWDNVRALLGGKGANLADMTRIG
                     LPVPPGFTVTTAACNAYLAEGEKFPAGMWEQELEALKHVEEVTGKKFGDPQNPLLVSC
                     RSGAKFSMPGMMDTVLNIGLNDNTAAGMVKLTGDERFVYDSYRRLVQMFGSVVLGISD
                     EAFEDPLEAYKHGKGIQSDTDLTAEDWKQLTEVFKQVVHKEQGFPFPQEPLEQLRLAT
                     EAVFKSWNGKRAIDYRNASGIAHDLGTAVNIVTMVFGNMGWDSGTGVAFTRNPANGEN
                     EMYGDYLLNAQGEDVVAGIRNTNPIAKLADEMPAAYKQFLEITEKLEKHYKDMQDVEF
                     TIEHNRLWMLQTRNGKRTAKAAVRVAIDMAKEGLISKEEAIMRVTPENVDTLLHPQFD
                     AMAKSNAKEQGNLVAKGVNASPGAAVGQIYFDADTSERMAKESKQDVIMVRPFTKPDD
                     VHGMLSAKGILTSEGGATSHAAVVARQFGVPCVVGASDIRIDLEKRVMTVSGKVVKEG
                     DWISVDGTTGEVYLGQIPTTSPNLEEQTDLLTLLGWADEVCSIPGQRQAPEGWPTRGL
                     QVWANADYPADARRARAYGAQGIGLCRTEHMFFEPARLPIVQRMILADSSEARTAALN
                     ELLPYQRADFEGLFNAMDGLPVIIRLIDPPLHEFLPSEEALLEEVITMRVKGETKDLD
                     AKEKLLASVRSLHESNPMMGLRGVRLSIEMPEIVEMQVRAIFEAAANQALAGVEVKPE
                     VMIPLTGTVKELEWIQPRLEKIAKAVMAEKGVTFDYKFGTMIEIPRAAVTAEEIAGLA
                     EFFSFGTNDLTQMTFGYSRDDAERNFLVTYVDQGILPKNPFQTLDREGVGRLMKMAIN
                     DGRKARPNLEIGICGEHGGDPASVEFCHMIGNNYVSCSPFRVPIARLAAAHAAITHRN
                     "
     gene            4395..4712
                     /gene="rplU"
                     /locus_tag="VN363_00730"
     CDS             4395..4712
                     /gene="rplU"
                     /locus_tag="VN363_00730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560893.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L21"
                     /protein_id="HWQ83055.1"
                     /translation="MKFAIVEDGGKQYRCVEGGTIDVDLLSAEPGEQVDLERVLLVAD
                     GDDVKVGTPLVEGAMIQATVVDQIKGPKLIVFRYKPKKRIRVKTGHRQKYTRLQIDSI
                     VLK"
     gene            4725..5006
                     /gene="rpmA"
                     /locus_tag="VN363_00735"
     CDS             4725..5006
                     /gene="rpmA"
                     /locus_tag="VN363_00735"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010545913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L27"
                     /protein_id="HWQ83056.1"
                     /translation="MAHKKGGGSSRNGRDSKGQRLGVKIFGGQTVRSGNILVRQRGTH
                     IKPGKNVGLGKDDTLFATADGVVVYETIRDDRKRVSVLEVAEVVAEKSE"
     gene            5008..5199
                     /gene="rpmE"
                     /locus_tag="VN363_00740"
                     /pseudo
     CDS             5008..5199
                     /gene="rpmE"
                     /locus_tag="VN363_00740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004811173.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing C-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L31"
     gene            5257..5481
                     /locus_tag="VN363_00745"
     CDS             5257..5481
                     /locus_tag="VN363_00745"
                     /inference="COORDINATES: protein motif:HMM:NF015102.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha
                     C-terminal domain-containing protein"
                     /protein_id="HWQ83057.1"
                     /translation="MDRHITDLELGPRPTDSLAKAGINTVGDVLTKMANGEAAILAIE
                     GFGRKSLADLKKRLRQFGYELPEAAEEITV"
     gene            5646..6218
                     /locus_tag="VN363_00750"
     CDS             5646..6218
                     /locus_tag="VN363_00750"
                     /EC_number="2.7.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004797 - thymidine kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thymidine kinase"
                     /protein_id="HWQ83058.1"
                     /translation="MKHHTGSIEVITGSMFSGKTDELIRRLRRATIARQKVQVFKPLI
                     DDRFSVEKVTSHAGGEFAAVPVQSAQEILGLLEPLTTVVAIDEAQFFDAAVIQLAQDL
                     ANRGMRVIVAGLDTDFRSEPFGPMPTLMAKAEIVDKLHAICMVCGESASRTQRLVNGK
                     PAYYDDPVVIVGASEMYEARCRKHHEVPHK"
     gene            6230..6784
                     /gene="hpt"
                     /locus_tag="VN363_00755"
     CDS             6230..6784
                     /gene="hpt"
                     /locus_tag="VN363_00755"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559827.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWQ83059.1"
                     /translation="MQSYEEYLDEILISEKDLQERIAELGAQITEDYRGHKLHLICIL
                     RGGVMFLTDLMRSIKIPTTIDFMAVSSYGNNRQSTGQVRITLDLKDNIQDRDVLLVED
                     IVDSGYTIASVLDFLKTRHPNSLKVCALLNKPERREVEVPIDYCGFNIPNKFVFGYGL
                     DIDEYHRNLPFIASVDLERYKPAG"
     gene            6791..>7210
                     /locus_tag="VN363_00760"
     CDS             6791..>7210
                     /locus_tag="VN363_00760"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83060.1"
                     /translation="MTPEPEPQSPTPDERAAYAGRWIALAGRQVIGQGGTPGQARRAS
                     QAARHKESLEVAYVPTPQPFAFSPILESVRTLASPDEPVYLVGGAVRDALLGQTSHDL
                     DFVLAGDVLGLARRTANLLGGAYFTLDGERQTGRVILT"
BASE COUNT         1493 a         1971 c         2037 g         1709 t
ORIGIN      
        1 ctctactatc cactgctggt gatcatgctc gtagccctgg ccctggcggc tgccgggttg
       61 ggagctctgc tggtgatggg ggttgtccgc atctttcccg cccggcgggt agctgaggtg
      121 ctgggcttca tcggcgcgat cagctcattc atctgctcgc agtccggtca gctggctaat
      181 tttgaggatg tacccccgga tcaggccatg cagatgatgg ccaatatctc gaggttttct
      241 tcaccctggt caccattgag ctggcctgga gaggctttag tgcgcttggg aagcgggcag
      301 tggctggtcg ggcttggctt tttggtgctg accctgggtt tgtgcggttt attattcttc
      361 tccagcctgg gggtggctga aaggctctat tacagcggct gggcgaacat gcagaataac
      421 cggcgcaagc ggaagcccac ctccactgcc gcgaccgccc aggctcagcc ctctccgttg
      481 tcgggtttgc tctcccagcc cctgcgggca atcctggtca aagattattt catgctgcgg
      541 cgtgagctgc gcaacatgtc acagctggtt accccgctca ttttcgggat tgtgtatgcg
      601 gtgatgctct tacggggcga tgatccactg ccggcacctg acagcgaaac cccaaccgtg
      661 ctggcagagg caatgcaaaa tatgcctcta tatttgagtg tggctttggc gatgtttgtg
      721 agctggatgc tggtgagccg cctggcaggg atgggttttt ctcaggaagg gaagaattac
      781 tggctgttga aaacagctcc cctcacttcc aaacagctct tactggcaaa attcctggtg
      841 gcttttctgc cttccttgat cctgggatgg atctttttgc tgatcatatc catgctgcgt
      901 ccgggtggcc tgagccagct ctggttcact ctgctggttg tcgccctgac gacggctgcc
      961 aacaccggga ttaacctggc ctttggtgtc gccggtgcaa atatgacctg ggaagacccg
     1021 cgcaagatga ccaccggtat gaacggttgt ctcgccagcc tggcgtcatt gatatttcta
     1081 ccggtctgcc tggggttgtt ctttagtccg cctatcttaa ttcccctggt agtagacaca
     1141 ccgccatttg tgggacagct ggtcgggctc ctgctgggag gaattgtctg cctggcggca
     1201 acggtgctgc ctccaaggct ggtgcaggcg cgggtgcccg tgctggatga gcctgccctc
     1261 tagcgccagg gtggaatcca tcgtcaccag aatacaaaga tcggggctgc cagctggcag
     1321 ccccgatctt tagatagaaa cgagaaaatt agttgcggtg ggtgatcgcc gcgtgggcag
     1381 cagccaggcg ggcaatcggt acccggaacg gcgagcagct cacgtagtta ttgccaatca
     1441 tgtggcagaa ctcgaccgaa gctggatcac cgccgtgctc accgcagata ccaatctcga
     1501 ggttggggcg ggccttgcgc ccgtcattga tcgccatctt catcaggcgt cccacgcctt
     1561 cccggtccag ggtctggaag gggttcttgg ggagaattcc ctgatcgacg tatgtcacca
     1621 ggaaattgcg ctctgcgtcg tcccggctgt agccgaaggt catctgggtc aggtcattgg
     1681 tgccgaagga gaagaattct gccagtccgg cgatttcttc agcggtcact gctgcacggg
     1741 ggatttcgat catggtgccg aacttataat cgaaggtgac acctttttca gccatgacag
     1801 ctttggcgat tttctccagc cggggctgga tccattccaa ttctttcacc gtgccggtca
     1861 gggggatcat cacttccggt ttcacttcga cacctgccag cgcctggttg gcggcggctt
     1921 caaagatcgc ccgcacttgc atttctacga tctcgggcat ctcaatgctc aggcgcacgc
     1981 cgcgcaggcc catcatcggg ttggattcgt gcagggagcg cacgctggcg agcagcttct
     2041 cttttgcgtc caggtctttg gtctcacctt tcacacgcat cgtgatgact tcttctaata
     2101 gggcttcttc agaaggaagg aactcgtgca ggggtgggtc gatcaggcgg atgataaccg
     2161 gcaggccatc catggcattg aacaggccct cgaaatcggc gcgctggtac ggcagcagct
     2221 cattcagggc agctgtgcgt gcctcagagg aatctgccag gatcatgcgc tggacaatcg
     2281 gcaggcgggc tggctcaaag aacatgtgct cggtgcggca cagacctatg ccctgagcgc
     2341 cgtaagcgcg agcgcggcga gcatccgccg ggtaatccgc attggcccac acctgcaggc
     2401 cgcgagtcgg ccagccttcc ggtgcctggc gctgtcctgg gatggagcag acttcatctg
     2461 cccatcccaa aagggtcagc agatcagttt gttcttccag gtttggagag gtcgtgggaa
     2521 tctggcccag atagacctca ccggttgtac catcaaccga gatccaatcg ccttccttca
     2581 ccaccttacc acttactgtc ataacccgct tttcaaggtc gatgcggata tcactggcgc
     2641 ccaccacaca gggcacgcca aattgccggg cgaccactgc ggcgtgggag gtagctccac
     2701 cttcgctggt cagaatgcct ttggcagaca gcatcccgtg gacatcatcc ggcttggtga
     2761 agggtcgcac cataatcaca tcctgcttgg attctttggc catgcgctcg gatgtatcgg
     2821 cgtcgaagta gatttgtccg acggctgcgc ccggggaagc attgacacct ttggcgacca
     2881 ggttaccctg ttctttggcg ttcgatttag ccatggcatc gaattggggg tgcaggaggg
     2941 tatctacgtt ttcaggggtt acccgcatga tcgcttcttc tttgctgatc aggccctcct
     3001 tggccatgtc gatcgccaca cggactgcag ctttggcggt gcgcttgcca ttccgggttt
     3061 ggagcatcca caggcggttg tgttcgatgg tgaactcaac gtcctgcata tctttgtagt
     3121 gtttttcgag tttctcggta atttccagga actgcttgta ggctgcgggc atctcgtctg
     3181 ccagtttggc aatcggattg gtgttacgga taccggcaac cacatcctca ccttgcgcat
     3241 tcaacaggta atcaccgtac atttcgttct cgccgttagc ggggttgcgg gtgaaagcca
     3301 cccccgtgcc cgagtcccag cccatattac cgaaaaccat agtcacgata ttgacggcag
     3361 tgccgaggtc atgggcgatc cccgaagcat tgcgataatc gatggcgcgc ttgccgttcc
     3421 aggatttgaa aacagcttct gtcgccaggc gcagctgctc aagaggctcc tgggggaaag
     3481 ggaaaccctg ctccttgtgg acaacctgct tgaagacctc ggtcagttgt ttccagtcct
     3541 cagccgtcag atcggtgtcg gattggatgc cttttccatg cttgtaagct tccagaggat
     3601 cttcgaaagc ctcgtcggag atacccagca cgaccgagcc gaacatctgg accaggcggc
     3661 ggtatgaatc atatacgaag cgctcgtccc cggtcagctt gaccatcccg gcggcagtgt
     3721 tatcgttcaa accgatgttg agcacggtat ccatcatgcc tggcatggaa aattttgcgc
     3781 cggaccggca agataccagc aggggattct gtggatcgcc gaattttttg ccggtcactt
     3841 cttcaacatg cttcaacgct tccagctctt gctcccacat tccggcaggg aatttttcac
     3901 cttctgccag gtaggcattg caggcggctg tggtcactgt gaatccaggg ggtactggca
     3961 gaccaatccg ggtcatatca gccagattgg cgcctttgcc gcccagcagt gctcgcacat
     4021 tatcccactt gccaccaact tctttctcag cttcttctac ttcttcaaaa agaaaaaccc
     4081 acttcttatc catatcaatt ctcctgattt agggttgtat aatagcaaca atccaacgga
     4141 atagtttacc acaaaagcta tttccgattt tcacgaaaga aagcttgctc atcagattct
     4201 aaatcacacg aatcatcagt tgacaggtta ctccaacccc ggtataatac cgtccgcact
     4261 ctagccccgg ctgttagctg ctggggcgct attctgtatc ccaaacttcc ccgcacacag
     4321 gcgcttcgtc agggagtttt gagcactcac acaaatgcgc gtggcgtgtg gtgaagtgaa
     4381 gcttggagaa tcaaatgaaa tttgctattg tcgaagatgg cggtaaacaa taccgctgcg
     4441 tggagggagg caccatcgat gttgacctct tatccgccga acctggcgaa caggtagatt
     4501 tggaacgtgt cctgcttgtc gctgatggtg atgatgtcaa agtaggtact ccgctggtcg
     4561 agggcgccat gatccaggcg actgttgtag accagatcaa aggccccaag ttgattgtgt
     4621 ttcgctataa gcccaagaaa cgcattcgcg ttaagactgg tcatcgccag aaatatactc
     4681 gcctgcagat cgactcgatc gtgctgaaat agaggaaggt aaccatggca cacaaaaaag
     4741 gtggcggctc gagccgcaat ggtcgtgata gtaagggtca acgcctgggc gtgaaaatat
     4801 tcggcgggca gacagtgcgc tccggcaata ttttggttcg ccagcgtggg actcacatca
     4861 agccaggtaa gaacgtaggc ctgggcaaag acgacacatt gtttgcaacc gcggacgggg
     4921 ttgtggttta cgagaccatc cgcgatgacc gcaaacgcgt ctctgtttta gaggtagccg
     4981 aggtcgttgc agaaaagagc gaatgaaatg aaaccagata tccatcccac ctattacacc
     5041 gaagcccagg tgatttgcgc atgcggcaat acctggacga ccggttctac ccgcaaggtc
     5101 atccataccg atgtgtgctc caaatgtcac ccattcttta caggtgagca gcgcattgtc
     5161 gataccgaag gccaggttga tcgcttctac aagaagcttc aggctcgtga gcagtacctt
     5221 accgatgtcg aaacccgcgc aactgcacgc acttccctgg accgccatat tactgatctt
     5281 gagctgggtc cacgtccaac cgacagcctg gcgaaagctg gcattaacac cgtcggtgat
     5341 gtgctgacaa aaatggcgaa tggtgaagcc gcgatcctgg caattgaagg ttttggtcgc
     5401 aaatccctgg cggatctgaa aaagcgtttg cgccagtttg gttacgagct gccagaagct
     5461 gccgaagaaa ttacggtctg atctctggct gaatcgccgg tcaacgggca ggtgtaggaa
     5521 tacgcctgcc tgtttttatt ttttttctgg cgggtatgcg tctggcgatc ctcgcttatt
     5581 caattggcgc gagcgacctc caggcggtaa aatatggcat ccatcacccc tgaccgagga
     5641 gttttatgaa gcatcataca ggctcaattg aagtcataac cggctccatg tttagcggta
     5701 agaccgatga attgatccgc cgtttgcgcc gcgccaccat agcccgacaa aaagtacagg
     5761 tctttaagcc cttgatcgat gatcgttttt cggtagaaaa agtcacctcc catgccggcg
     5821 gtgaatttgc agcagttcct gtccagtcgg cgcaggagat cctcggcttg ctcgagccgt
     5881 tgaccacggt ggtagcgatc gatgaggccc agttttttga tgctgccgtt attcaattag
     5941 cccaggacct ggccaaccgc gggatgcgcg tgatcgtcgc cgggctggat acggatttcc
     6001 gcagtgaacc ctttggtccg atgccgaccc tgatggcaaa ggcggagatt gtggataagc
     6061 tgcacgccat ctgcatggtg tgcggcgaat ctgccagccg cacccagcgc ctggtgaatg
     6121 gaaagcctgc ttattatgat gacccggtgg tcattgtggg tgcttccgaa atgtacgaag
     6181 cgcgctgccg caagcaccac gaagtacccc ataaataaag aggtgtatcg tgcagagtta
     6241 tgaagaatat ttagatgaga tcctgatctc tgaaaaggat ctgcaggagc gcatcgccga
     6301 gcttggggcg cagatcaccg aggattatcg cgggcataag ctgcatttaa tctgcatttt
     6361 gcggggtggg gtaatgtttc tcaccgactt gatgcggtcg atcaagatac ccacaaccat
     6421 cgatttcatg gcagtttcat cgtacggcaa taaccgtcag agcaccggtc aggtgcgcat
     6481 caccctggat ctgaaagaca acattcagga tcgggatgtg ctgctggtcg aggatatcgt
     6541 cgacagcggt tacaccattg cctctgtact ggattttctg aaaactcggc atccgaacag
     6601 cctgaaggtg tgcgccttac tgaataaacc cgagcgccgt gaagtggaag tgccgatcga
     6661 ttactgcgga tttaatatcc ccaataaatt tgttttcggc tacggcctgg atatcgatga
     6721 gtaccatcgc aatttgccgt ttatcgccag cgtggacctg gagcgctata agccggctgg
     6781 gtgacccggc atgacacccg agcccgagcc tcaatcgccc acccctgatg agcgcgccgc
     6841 gtatgcgggg cggtggatcg cgctggctgg caggcaggta atcgggcagg gtggcacacc
     6901 tgggcaagcc cggcgagcct cccaggctgc ccggcacaag gaatctctcg aggtcgcata
     6961 cgtgcctacc ccacaacctt ttgcattttc acctatcctg gagagcgtgc gtacgctcgc
     7021 ctcaccagat gagcctgtct atctggtcgg cggcgcagtg cgtgacgctc tcttaggcca
     7081 gacatctcac gacctggatt ttgtgctggc aggggatgtg ctggggcttg cccggcggac
     7141 cgctaatctc ctcggtgggg cgtatttcac cctggatgga gagcgccaga cgggaagagt
     7201 tattttgacc
//
LOCUS       DATMXK010000038         4744 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_54870, whole genome shotgun sequence.
ACCESSION   DATMXK010000038 DATMXK010000000
VERSION     DATMXK010000038.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4744)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4744)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4744
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54870"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(20..1420)
                     /gene="lpdA"
                     /locus_tag="VN363_00765"
     CDS             complement(20..1420)
                     /gene="lpdA"
                     /locus_tag="VN363_00765"
                     /EC_number="1.8.1.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004148 - dihydrolipoyl dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0045454 - cell redox homeostasis [Evidence
                     IEA];
                     GO_process: GO:0051068 - dihydrolipoamide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoyl dehydrogenase"
                     /protein_id="HWQ83061.1"
                     /translation="MSDYDVIVIGAGPGGYICAIRCAQLGLKTAIVDKQWLGGVCLNV
                     GCIPSKALLKNAEVAHTLRERGKEFGFSFDNLVLDYSVAVKRSRSTSERLVKGVGFLM
                     KKNKIDVHMGAGKLTATDTVQVTAEDGKTTELKAKNIVVATGASVMIPPGWNVDGKKV
                     VTYLEAILQTELPKSVVIIGAGAIGVEFATVWNSYGVDVTIVEMLPHLLPLEDEEVSS
                     ELEKAFTKRKIKSLTNYRVEGIETTDSGVKVIVSSEGQEKKVLEADQALVAIGFKPNG
                     RGLGLEEAGVKLSERGAVEIDERMATNVPGIWAIGDATAKLMLAHVGSAQGIICAENI
                     AGAETTTLNYEMMPRAVYCQPQVASFGMTEAQAKERGYEIKIGRFPYQANGKALGLGE
                     STGWVKLITDAKYGEILGAAMIGPEVTELLPELTLAQMMELTAEEIARNVHAHPTLSE
                     ALMEAAHAAEGRSINI"
     gene            complement(1423..2742)
                     /locus_tag="VN363_00770"
     CDS             complement(1423..2742)
                     /locus_tag="VN363_00770"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWQ83062.1"
                     /translation="MSTQVLMPRLGESVDEGTISKWLKAIGDPVAQYEPLLEVNTDKV
                     DSEIPSPAAGVVLEILVPEGTLVQVGTPLARIGAPGEAASGGDQPERASTPPPATETA
                     SVTAPAPQAAPSTPAAVPGAGKDRELGFISPVVAKIARQENLDLRQVPGSGQAGRITK
                     QDVLDYLEKRPGAPALAQPPASSLPSIHPSISQPVPAGSAAVSPGKILPLNNVRKAIA
                     EHMVLSKHTSPHVTTVMEADLSQVIAHRNANKEQYARDGVNLTFTGYFMAATVTALKA
                     YPIVNSSWNEAGIVLHPQINVGMAVSLGDEGLIVPVIKNADGLSLLGLARMINDLASR
                     ARGHQLKPDEVKGGTFTITNHGISGSLFASPIINQPQCAILGVGAIQKRVVVVSDPVQ
                     GDTIGIRSMAYLTLTFDHRILDGAIADYFLGKVVESLQTWNIQSRGA"
     gene            complement(2783..>4744)
                     /locus_tag="VN363_00775"
     CDS             complement(2783..>4744)
                     /locus_tag="VN363_00775"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ83063.1"
                     /translation="GLAYASVLYREVPELSAFRQFSDHGNEVAFGTIGNASTAEGMFW
                     EAVNAIGVLNAPAVISIYDDGYGISVPNLYQMVKQDLSTILKGFGRWEGSCDGFDLYT
                     VRGCDYPALYQTYQQAAANARVDHTPAILHITELTQPQGHSTSGSHERYKSKERLEWE
                     VENDVLRCMRQDVISTGVVDDKTLAEMEAEERQFVESERQAAWEAYQAPILKERQELI
                     GLLSTLAGESQQPEILKGIQEKLAGLPQPLRRDLLSEAHTALRATRSQTLGSKRTLIQ
                     WRDQLQATTQERYSSLVYSESPESALRVPVVPAEYAPDAQQVYGYEILTACFDAALGR
                     DPRIFAFGEDVGQLGDVNQGMRGLQEKYGLYRVADTGIREATILGQAIGMALRGLRPI
                     AEIQYLDYLLYALQIMSDDLATLLWRTHGGQKAPVIVRTRGHRLEGIWHSGSPMASII
                     HLVRGMHVLVPRNMTQAAGFYNTLLRSDEPGLIIEVLNGYRLREKLPANIGEFTVPLG
                     EPEILRAGKDITLVTYGACCRLALEASNLLAETGVDTEVIDVQSLLPFDIQGRIGESL
                     QKTGRVVFVDEDMPGGTTAYMLQEVLEKQGGYQWLDSAPTTVPARPHRPAFGSDGDYF
                     SKPNVEQIFDAVYTLMHESNPARYPLFDR"
BASE COUNT          930 a         1388 c         1346 g         1080 t
ORIGIN      
        1 cagctaaata ttatccgggc tagatattga tgctgcgccc ttctgcagca tgggcagctt
       61 ccattagagc ttcgctcaat gtggggtggg cgtgaacgtt gcgggcaatc tcttcagcag
      121 tcagctccat catctgagcc agagtcagct ccgggagcag ctcggtcact tcgggcccga
      181 tcatggcagc cccaaggatc tcgccatact tggcgtcggt gataagcttc acccagccgg
      241 tggattcacc cagccccagg gctttgccgt tggcctggta agggaagcgc ccgatcttaa
      301 tctcatagcc gcgttctttg gcctgtgcct ctgtcatccc gaaggaagcc acctgtggct
      361 ggcagtatac ggcccgcggc atcatctcat aattcaaagt agtggtctcg gctccggcaa
      421 tattctcggc gcagataatt ccctgggccg agccgacatg ggccagcatc agcttggcgg
      481 ttgcgtcgcc gatcgcccag atgcctggca cattggtcgc catgcgttcg tcaatctcga
      541 ctgcgccgcg ctcgctcagc ttcacaccgg cttcttccag acccaggccg cgtccgttcg
      601 gcttgaatcc gatcgctacc agagcctgat cggcttcgag aacctttttc tcctgtccct
      661 cgctggagac aatgactttg acgccgctat ccgttgtttc gatgccttct acccgatagt
      721 tggtcaggga tttgatcttg cgtttagtaa aagctttttc taactcgctg gaaacctctt
      781 catcttccag aggcagcagg tggggaagca tttctacaat ggtcacatcc actccgtagc
      841 tgttccatac ggtcgcaaat tctactccga tggctccggc gccgatgatg acgactgatt
      901 tgggcagctc tgtttgcagg atggcttcga ggtaggtgac cacttttttg ccatccacat
      961 tccaacccgg tgggatcatg acgctggccc cggtggctac gacgatattc tttgctttca
     1021 gctccgtggt tttcccgtct tcggcagtga cctgaacagt atctgtggcg gttagcttcc
     1081 cggcgcccat gtgaacatcg atcttattct ttttcatcaa gaagccgacc ccttttacca
     1141 gccgctcgga tgtggagcga ctgcgcttga cggccacact gtaatccagg accaaattat
     1201 caaacgaaaa accaaattcc ttcccccgct cgcgcagcgt gtgagcgact tcagcgtttt
     1261 tgagcaaagc tttggaggga atgcagccca catttaaaca aactccccct aaccattgtt
     1321 tatcgacgat ggcggttttc aaacccaatt gagcacaacg aatggcgcaa atatacccgc
     1381 ccggtccggc gccaatcaca atgacatcgt aatcgctcat tttcaagctc ctctagattg
     1441 tatattccag gtttgtaagg attctaccac cttgcccagg aagtaatcgg cgattgctcc
     1501 atccaggatg cggtggtcaa aggtgagcgt cagatacgcc atcgagcgga tgccgatcgt
     1561 gtctccctga acggggtcac tcaccacgac gacccgcttc tggatggcgc caactcccag
     1621 gatagcacac tgcggctggt tgataatagg tgaggcgaac agcgagcctg agattccatg
     1681 attcgtgatc gtaaaagtcc ctcctttgac ttcatccggt ttgagctggt gcccccgcgc
     1741 ccggctggcg aggtcattga tcatgcgcgc cagtccgagc agggaaagtc cgtctgcgtt
     1801 cttgattact ggcacgatca atccctcatc ccccagagaa actgccatgc caacattgat
     1861 ctggggatgc agtacaatgc cggcctcgtt ccaggatgaa ttgacgatcg ggtaggcttt
     1921 aagcgcagtt acggttgccg ccatgaaata gccagtgaat gtcaggttga ccccatcccg
     1981 ggcatactgc tctttattgg cgttgcggtg ggcgatcacc tggctcagat cggcctccat
     2041 caccgtggtt acatggggcg aggtgtgttt gctgagcacc atatgctcgg cgatcgcttt
     2101 gcgcacgttg ttaagcggca ggattttgcc ggggctgacc gcggcgctcc cagcagggac
     2161 aggctggctg atgctggggt ggatcgaagg tagagaggaa gccggcggct gggccagcgc
     2221 aggtgcgccg gggcgctttt cgaggtaatc cagcacgtcc tgcttggtga tccgcccagc
     2281 ctgcccgctg ccgggcacct ggcgcaagtc caggttttcc tggcgggcaa tttttgccac
     2341 gaccggtgag ataaacccca gctcacggtc cttgccggct ccggggacgg ctgcgggtgt
     2401 gcttggagcg gcttgtgggg ctggtgcagt cacactggct gtttcggtgg caggtggtgg
     2461 ggtgctggcc cgttcaggct ggtctccgcc tgaagccgct tcgcccggtg caccgatcct
     2521 cgccagtgga gttccgacct ggacgagcgt tccttccgga accagaattt ccagcacgac
     2581 tccagcagcc gggctgggga tttccgaatc gactttatcg gtattcactt cgagcaaggg
     2641 ctcgtattgg gcgacaggat cgcctatcgc tttcagccat ttgctgatcg tgccttcatc
     2701 taccgattcg cccaacctgg gcatcagaac ctgtgtggac atgcagcctc cagaaaagga
     2761 tatcgtgcgg gatcacgcag ttttagcggt caaacagtgg atagcgtgcc ggattggatt
     2821 catgcatcag tgtgtatacg gcatcaaata tttgctccac attgggctta gaaaaataat
     2881 cgccatcgga gccaaatgcc gggcgatggg gccgggcggg gactgtggtt ggcgccgaat
     2941 cgagccactg gtagccacct tgcttttcca ggacctcctg gagcatatag gcggtcgtcc
     3001 caccgggcat atcttcgtcc acaaagacga cccggcctgt cttttgcagc gactcaccga
     3061 ttcgcccttg gatgtcaaag gggagcaggc tttgtacatc gatcacctca gtatcgacgc
     3121 cggtttccgc cagcaggttg ctggcttcca gcgccagccg gcagcatgcc ccgtaggtca
     3181 ccagggtaat gtccttgcca gcgcgcagga tttcgggttc tcccaaaggc acagtaaact
     3241 caccgatatt ggcgggcagc ttttcgcgca gccggtaacc attcagaact tcgatgatca
     3301 agccgggctc gtccgagcgg agcagggtat tgtagaatcc agctgcctgg gtcatattgc
     3361 gcggcaccag cacgtgcatc ccgcgcacca ggtgaatgat gctcgccatg ggagagcctg
     3421 aatgccagat gccctcgagg cggtgcccgc gggtgcgcac gattaccggg gctttttgcc
     3481 cgccgtgggt gcgccagagg agagtagcca gatcgtcgga cataatctgc agggcgtaga
     3541 gcaagtagtc caggtattgg atttcggcga taggacgcag gccgcgcagg gccatgccga
     3601 tcgcctgacc cagaatggtt gcctcccgga tgccggtatc cgcaacccgg tacagaccat
     3661 atttttcctg caggccgcgc atgccctgat tcacatcgcc cagctgccca acgtcctctc
     3721 caaaggcgaa tatgcgtggg tcgcgtccca aggcagcatc gaagcaggca gtgaggatct
     3781 catatccata cacctgctgg gcgtctggag catattctgc cggaacaacg ggaacccgga
     3841 gagccgattc gggggattca ctgtacacga gcgagctgta cctctcctgg gttgtcgcct
     3901 ggagctggtc gcgccactgg atcagagtgc gtttactccc cagagtctgg gagcgagtag
     3961 cgcgcagggc tgtgtgcgcc tcgctcagca ggtcgcggcg cagaggctgt ggcagacctg
     4021 ccagcttttc ctggatcccc ttaaggattt caggctgctg cgattcgcca gccagggtgg
     4081 ataataaccc gatcagctcc tggcgttctt ttagaatagg cgcctggtaa gcctcccagg
     4141 ctgcctggcg ctccgattca acgaattggc gttcttcagc ttccatctct gccagggttt
     4201 tatcgtccac aaccccggtc gatattacgt cctggcgcat gcagcgcagg acatcattct
     4261 ccacttccca ttccaggcgt tccttggatt tataccgctc gtggctgcca gaggtggaat
     4321 gtccctgcgg ctgggtaagc tcggtaatgt gcaggatcgc cggggtatgg tctacccgag
     4381 cgtttgcagc agcttgctgg taagtctggt agagggccgg gtagtcacac ccgcgcacag
     4441 tatacaggtc gaagccatca cagctcccct cccagcgccc aaaacctttc aggatcgtag
     4501 aaaggtcctg cttcaccatc tggtacagat tagggacgga gataccgtaa ccgtcatcgt
     4561 aaatcgagat tactgccggg gcgttcagca cccctatggc attaaccgcc tcccagaaca
     4621 tcccttcggc ggtggaagca ttgccgatgg tcccgaatgc cacttcattc ccatggtccg
     4681 aaaattggcg gaaagcgctc aactcgggta cttcccggta caggacagag gcatacgcca
     4741 accc
//
LOCUS       DATMXK010000039         2036 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_123796, whole genome shotgun sequence.
ACCESSION   DATMXK010000039 DATMXK010000000
VERSION     DATMXK010000039.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2036)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2036)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2036
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123796"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1706
                     /locus_tag="VN363_00780"
     CDS             <1..1706
                     /locus_tag="VN363_00780"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HWQ83064.1"
                     /translation="QLTIKADLILLHAPSVFDFRQRDDVLFAYLSDSDSVNVTSIFEM
                     YPLGFLSLQAHLQQHGIKVVIINLASLMLQHSNLDIDRLLAHLEAPVFGLDLHWMAHC
                     HGSIEVAQRVKAIHPDSLVIFGGLSATYFADQLIQYPAIDLVVQGYDTLEPVRQLVQS
                     VIDGSRNLAAIPNLVYRQPDKTVAKTGYTHQPSRKYNDAAIQWSFYRDAISSPTVNRL
                     IMTLPNSGCAHDCGWCGGSRSAFVQPTGTHRSLVYKDHKHIVAELASMADAARQTSIY
                     ALQCYSEPPQRMYEYLDTIQDMGYKSVSFELYNLPDEKLTRRIAQATQAYVLLSPESH
                     DQEISRLAGRGNYSMAQMEAWIEKALDSGIAGVMVWFFIGMPRQTSESVMETVNYCER
                     LIRRFPGRKVIPLLCPMVPFLDPGSRFYEKPEAHGYRIFFHDLEQYRQAMVAPLWYQR
                     LNYETDWLSRRQLQNVTYESISRLVEIKGELGMLPKSFTRGILMTIDETQSLLAEIES
                     VIQSGAQLTSDLRAQIHLYNQKILATSSDQIVPIPRPIGSRWFDDMAVSQEIIKDCII
                     A"
BASE COUNT          474 a          577 c          476 g          509 t
ORIGIN      
        1 gccaattaac aattaaagct gacctgatcc ttctgcacgc gcccagcgtg ttcgattttc
       61 gacaacgaga cgatgtccta ttcgcatacc tgagtgattc ggacagcgtg aacgtcacat
      121 ccatttttga aatgtaccca cttggtttcc tatccttgca agcacatttg cagcagcatg
      181 ggattaaggt agtaatcatc aacctggctt cattaatgct gcagcactcc aacttagaca
      241 tcgatcgcct gctcgctcac ttggaggctc cagtatttgg gcttgacctt cactggatgg
      301 cgcactgtca tggctcaatc gaagtcgccc aacgagttaa ggctatccat cctgatagtc
      361 tggtgatctt cggcgggttg tcggccacct actttgccga tcaattgata cagtatccag
      421 caattgatct ggttgtgcag ggttacgaca ccctggaacc ggtgcgccag ctggtgcagt
      481 ctgtcatcga tggcagtcga aacctggcgg caattcctaa cctggtctac cgccaaccag
      541 acaagacagt ggcaaaaact ggctacaccc accagccatc cagaaagtac aatgatgctg
      601 ctattcaatg gtccttttat cgcgatgcca tctcttctcc cacagtaaat agattgatta
      661 tgaccctacc caatagtggc tgcgcccacg actgtggctg gtgtggtggt tctcgctctg
      721 ctttcgtaca gcctactggc acccaccgtt cattggtgta taaagaccac aagcatattg
      781 tcgccgagtt ggccagcatg gcagatgccg cccggcaaac ttcgatttat gccctccagt
      841 gttactccga gcctccccag agaatgtatg aatacctgga tacaatccag gatatgggtt
      901 acaaatctgt atccttcgag ctatacaacc tgccagacga gaaactcact cgccgtatcg
      961 cccaggcaac ccaggcgtat gtgctgctgt cacctgaatc acacgaccag gagatcagcc
     1021 gtctggccgg tagaggtaat tattcaatgg cgcagatgga agcctggatc gaaaaagcgc
     1081 tggactcagg tatagctggg gtgatggtct ggttcttcat tgggatgccg cgccagacgt
     1141 ccgaatccgt gatggagaca gtgaattact gcgagcggct tatacgccgt tttccgggtc
     1201 gcaaagttat tccgctgttg tgcccaatgg tgccgttcct ggatcccgga tctcgtttct
     1261 atgaaaagcc agaagcacac ggctaccgta tcttcttcca cgatcttgag cagtaccgcc
     1321 aggcgatggt agcgccatta tggtatcagc gcctaaacta tgaaactgat tggctttctc
     1381 gccgccagct ccagaacgta acctacgaaa gcatttcccg gctggttgaa atcaaaggcg
     1441 agcttggcat gcttcccaaa agtttcaccc gcggtatctt gatgaccatc gacgagaccc
     1501 agtcactgtt ggcggagatt gagagcgtta tccaatcagg agctcaatta acatctgacc
     1561 ttcgagctca aatacacctc tacaaccaga agatcctggc cacttccagc gaccagatcg
     1621 tacctattcc aagacctatc ggcagtcgct ggttcgatga tatggcggta tcgcaggaga
     1681 tcatcaaaga ttgcattatt gcctgacaaa tttttcaaga aatactaaat aagttactgg
     1741 catgaggatc atgattccga tggcgtttca cattgtccca ctcgcacccc aacacctccc
     1801 cgccgtggct cagctcgttt ccaggaagta tcaatccctc cagattcagg tcagctgcct
     1861 gcctgctccc gctcgtccag gagatctctc gcgccaaccc ctctgctgcc gcctgtatta
     1921 attaaaaacc gctcaatttg tgttctttct catcccgctg tcctcccgct cgagattcac
     1981 tccagctcag gcggcccttt ttgggcggcc ctttttgggc agccctgctc agggag
//
LOCUS       DATMXK010000040         2531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_88105, whole genome shotgun sequence.
ACCESSION   DATMXK010000040 DATMXK010000000
VERSION     DATMXK010000040.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88105"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1434
                     /locus_tag="VN363_00785"
     CDS             <1..1434
                     /locus_tag="VN363_00785"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HWQ83065.1"
                     /translation="NIPPPADPALDSVHLQTTLPELAGRVPDVLNRYQLDVSITPEQL
                     SFQGNLVLDYTNLESTALGTLFFRLLPNGQRSYGNGSLSVDNVQVNGENQAVNLSLED
                     TILEVTLDPALEPGASATIAMNFQGEVPEDFGGEDSGGYGIFNFSAEVMALASWYPIL
                     AVYDEAGWHLDPVSYIGDSVYSDMADYEVEIELPADQVLVSTGVARQTGAGGDRQLLV
                     VESGPVRDFFMILSPRFRSLQSRFGGTRVNSYYLPGHENGGQRALEVAVLSIETFNRQ
                     FGAYPYTELDVVDAPMQNAGGVEYPGIVLVGDSQYTDYNETGFAVTTAHEVAHQWWYN
                     LVGNDVFAEPWLDEALASYTSGIYLEAAQGKPALDGLMSHYQERYQRSLDSSGDHPIN
                     ESLAYFESQTNPNAYGGIVYAKGALFFDAVRQEIGDEAFFAAMRSYFDTYRYGIAAGK
                     DLLAAFEAAAGRPLAELFQTWNVASQP"
     gene            1465..2514
                     /locus_tag="VN363_00790"
     CDS             1465..2514
                     /locus_tag="VN363_00790"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWQ83066.1"
                     /translation="MILRRPHYSHPFSRVFALVCLGLFFAACTTAPTATLLVHTPTLQ
                     NTGTPLPGFPIPITRTPTPDTLPETAGPASVTPPAVTATPESPLRFAVIGDYGLAGEP
                     EAQVAQLISSWGPDLIITTGDNNYPDGEAETIDANIGQYYQEYIYPYYGDYGPGADRN
                     RFFPTLGNHDWYTPGAQPYLDYFTLPGNERYYEYTWGPVHFFAVSSDSREPDGVGRSS
                     VQAAWLQERLAAATLPWKVVYFHQAPFSSAYHGSVDWMRWPFKEWGASLVLAGHDHTY
                     ERLEIDGLTYIVNGLGGGPIYPFFAPLPGSMVRYNDTQGALLVEADAEQLRGYFANLD
                     GTNSDQFRLLIPNKP"
BASE COUNT          528 a          738 c          699 g          566 t
ORIGIN      
        1 aatatacctc ccccggccga tcccgctctg gattctgtgc atctccaaac aaccctgccc
       61 gagctggctg gcagagtccc ggatgtactc aatcgctacc agctggatgt atccattacg
      121 cccgagcagc tcagcttcca aggaaatctg gtcctggatt acaccaacct cgaaagcact
      181 gctttaggaa cacttttctt ccgtttgctg ccgaatgggc agcgcagcta tgggaatggt
      241 tcattgagtg ttgataatgt gcaggtgaac ggcgaaaacc aggcggtcaa cctgtcgctg
      301 gaagatacta ttcttgaagt tactctggac ccagccctgg agcctggcgc atcagccacg
      361 atcgccatga actttcaagg tgaggttccc gaggatttcg gcggtgagga ctcgggagga
      421 tatggcattt tcaacttcag cgcggaggtg atggccctgg caagctggta tcccatcctg
      481 gcagtctacg atgaggcggg ctggcattta gatcctgtat cctacatcgg cgattccgta
      541 tacagcgaca tggctgacta tgaagtagag atcgagctgc ctgcggacca ggtgctggtc
      601 agcacagggg tcgccaggca aacgggagca gggggtgatc gccagctgct ggtcgtggaa
      661 agcggtccgg tgcgcgattt cttcatgatc ttaagcccgc gctttcgcag tctccagtcc
      721 cggttcggcg ggacgcgggt caattcctat tatttaccgg gccatgaaaa cggtgggcag
      781 cgcgccctgg aggtcgccgt gcttagcatt gaaacattca accgccagtt tggggcttat
      841 ccatacaccg agctggatgt tgtggacgcc ccgatgcaaa atgccggcgg cgtcgagtat
      901 ccggggatcg tccttgttgg agacagccag tacacagact ataacgagac gggttttgca
      961 gtcacaacag cccacgaggt ggcccaccag tggtggtaca acctggtagg gaacgatgtt
     1021 tttgccgaac cctggctgga tgaagccctg gcgagctaca cttccggcat ctacctggaa
     1081 gccgcccagg gaaaaccagc cctggatgga ctgatgagcc attatcaaga acgctaccag
     1141 cgcagcctgg acagcagcgg tgatcaccca attaatgaga gcctggcgta tttcgagagc
     1201 cagaccaatc ccaatgctta tggggggatc gtatatgcca agggcgccct cttttttgat
     1261 gctgtacgcc aggagattgg ggacgaggct ttctttgctg ccatgcgcag ttattttgat
     1321 acttatcgtt acgggatcgc agccgggaaa gatctcctgg ctgcttttga agcggccgcc
     1381 ggccggccgc tggctgagct cttccagacc tggaacgtcg cttcccaacc gtaaactccc
     1441 tggttttcgg ctacaatcgt cgatatgatc cttcgccgcc cacactattc ccaccctttc
     1501 agccgggttt tcgctctggt gtgtctcggc ctgtttttcg cagcctgcac gacagccccg
     1561 accgccactc tactcgtcca tacacctacc ctccagaaca cgggaactcc cctccccggc
     1621 ttccccatcc ccataacgcg cacacctacc ccagataccc ttccagagac ggctggacca
     1681 gcttcggtca cgccacctgc tgtcaccgca accccggaat cccccctgcg gtttgcggta
     1741 atcggcgact atgggctggc gggtgagcct gaagcccagg ttgcccagct tatttccagc
     1801 tggggtccag acctgatcat taccacgggg gataacaact atccggatgg cgaagctgag
     1861 acgatcgatg ccaacatcgg acagtactac caggagtata tttatccata ctatggcgac
     1921 tatggacctg gagcagatcg caaccgcttc ttcccgaccc tgggcaacca tgattggtat
     1981 acccccggag ctcagccgta tctcgattat ttcaccctgc ccggtaatga gcgctattac
     2041 gagtatacct ggggaccggt ccatttcttt gcggtgagca gcgactcacg tgagcccgat
     2101 ggggttggcc gcagctctgt ccaggctgcc tggctgcagg agcgcctggc tgccgcgaca
     2161 ctcccctgga aagttgtcta cttccaccaa gcgcccttct cgtccgccta tcacggctca
     2221 gtagattgga tgcgctggcc ttttaaggaa tggggagcca gcctggtcct tgccgggcac
     2281 gatcatactt acgagcgttt agagatcgat gggctgacgt atatcgtcaa cggcctgggg
     2341 ggaggtccga tctatccgtt ctttgcgccc ctccccggca gcatggtccg atataatgat
     2401 acgcagggtg cactgctggt cgaagcggat gccgagcagc tgcggggata ttttgcgaat
     2461 ctcgacggga ctaattcgga ccaatttaga ttattaatac caaataagcc ttgacagcct
     2521 gaaaaagttc a
//
LOCUS       DATMXK010000041         2497 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_88068, whole genome shotgun sequence.
ACCESSION   DATMXK010000041 DATMXK010000000
VERSION     DATMXK010000041.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2497)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2497)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2497
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_88068"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VN363_00795"
     CDS             complement(<1..973)
                     /locus_tag="VN363_00795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83067.1"
                     /translation="MRPSRLIPIGLMSSVLILATLACSLFDDLPQIPGSAEAPIASST
                     PRPTPTALPAMPVQPGESNPDEPVYVYGDIPYTSPFFVNSLSEPYVLLEDEAGFVARD
                     REFEFKLPGQMIGPVEVHEDGSLTYSLPLPSVPLGTFVDVDNDGGADKGLQVFAAAYW
                     SNTWGGPFLERRDGTGWSTAYSSTLTDPENEDEIMGGILIVWSPDSEQAFPTGFGADG
                     LLFTADDPTAPIPAGYNIVDLDQEPFQVTKETRPEITLNEGVVAVNNYTQDDYATAFE
                     KLYEKVAREYPFTSEKNVDWQALYAEFSPRIADAGDDTDFYEALRDFT"
     gene            complement(1051..1641)
                     /locus_tag="VN363_00800"
     CDS             complement(1051..1641)
                     /locus_tag="VN363_00800"
                     /inference="COORDINATES: protein motif:HMM:NF019494.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyhydroxyalkanoate synthesis regulator
                     DNA-binding domain-containing protein"
                     /protein_id="HWQ83068.1"
                     /translation="MPLIKRYPNRKLYDTVARRYITLEEIAKLVQGGEDVKVIDHASG
                     MDLTELTLSQVVYELQKKKRRFLPHSLLAALIHDAVPPVSRVVGSFGLNELVAAEIQR
                     RIEALVTGGELTAEEGSLWLNKLLSNAPDQAETHSPWSYPGPFTPEQIEQVIQRLPIP
                     SRKEFRKLEEQLHQLEAALEQIKPPDTPDDPPEAND"
     gene            complement(1652..2017)
                     /locus_tag="VN363_00805"
     CDS             complement(1652..2017)
                     /locus_tag="VN363_00805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83069.1"
                     /translation="MTTETSEPRGSPHMKGDRPLYEATRRLLLASVGAVALAQDEIED
                     FINKLVERGELADKEGFKLMNEMKSHRSKHLRKAEEELTGHLEAAMTRMNLPTKADLD
                     ALSEKISTLNQRIEELKKE"
     gene            complement(2159..>2497)
                     /locus_tag="VN363_00810"
     CDS             complement(2159..>2497)
                     /locus_tag="VN363_00810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559855.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWQ83070.1"
                     /translation="DYDHERAIDTTRELLTQYLAQQPHIARASGVSMDVVAEIQSILG
                     WPATREQIQKAKHLVPEELIYKITASGTPDEARAKVEEYKKHGCTCPILYPVGGDVKL
                     LIDTFAPASA"
BASE COUNT          491 a          675 c          733 g          598 t
ORIGIN      
        1 aggtgaagtc gcgcaaagct tcatagaaat ccgtgtcgtc gcccgcatcg gcgatgcggg
       61 gagaaaattc ggcgtacagg gcctgccaat ccacattctt ttcggatgtg aaggggtatt
      121 cacgggcgac cttctcatat aatttctcaa aagccgtggc gtagtcgtcc tgggtgtaat
      181 tgttgaccgc gaccacccct tcgttcaggg tgatttccgg gcgggtttct ttggtcacct
      241 ggaacggttc ctggtccaga tcgacaatat tgtagccagc cgggatcggc gcggttgggt
      301 catcggcagt gaacagcaga ccatcggcgc caaatccagt gggaaaagcc tgctcgctgt
      361 ccggcgacca gacgatcagg atcccaccca tgatctcatc ttcattttcc gggtctgtca
      421 gagtcgacga atatgcggtg gaccaaccgg tgccgtcccg ccgttccagg aaaggcccgc
      481 cccaggtgtt ggaccagtaa gccgcggcaa agacctgcag gcctttatcg gctccgccat
      541 cattatccac atccacaaac gtgcccaacg ggaccgacgg cagcggcagg gagtaggtga
      601 gtgagccatc ctcgtggact tctaccgggc cgatcatctg cccgggaagc ttgaattcga
      661 attcccggtc gcgggcgaca aaccccgcct catcttcgag taagacatac ggttcggaga
      721 gtgaattgac gaagaagggc gaggtgtaag ggatatcccc ataaacatac accggctcat
      781 ctggattgct ctcccccggc tggacgggca tcgccggtaa agcggtcgga gtggggcgcg
      841 gggttgagct ggcgattggc gcctcagcag agccaggaat ctgcggtaag tcgtcgaaca
      901 ggctgcatgc cagggtcgcc agaatcagga cggagctcat gagaccaatg ggaatgaggc
      961 gagaagggcg catggttctc tccagttgga ataaatgcgt ggaattccca ccattatagc
     1021 tttatttggt ttattacagg tgagagcaag ttaatcgttt gcttctggag gatcgtccgg
     1081 cgtgtccggt ggcttgattt gttccaaagc ggcttccagc tggtggagtt gttcttccag
     1141 cttgcggaat tccttccggc tggggattgg caggcgttgg attacctgtt cgatttgctc
     1201 gggggtaaag ggtcctggat aagaccaggg agaatgtgtc tcagcctggt ctggggcatt
     1261 tgagagcagc ttatttagcc acaaactgcc ttcttcagcg gtcagttcac ccccggtgac
     1321 cagcgcttca atccggcgct ggatttcagc ggcgacgagc tcgttcaggc cgaacgagcc
     1381 gaccaccctg ctcaccgggg ggactgcatc gtggatcagg gctgccagca ggctgtgtgg
     1441 taaaaaccgc cgttttttct tctgcagctc atagactacc tggctgaggg tgagctcagt
     1501 cagatccatg ccgctggcat ggtcaataac cttcacatct tccccgccct gcaccagctt
     1561 tgcgatctct tccagcgtga tgtaacgtct ggctacggta tcgtaaagct tgcggttggg
     1621 gtagcgtttg atcagcggca tgtttgccgg gctattcttt cttgagctct tcgatcctct
     1681 ggttcagggt ggaaatcttc tcgctcagcg cgtccagatc ggctttggtg ggcaaattca
     1741 tgcgcgtcat ggcggcttcc aggtgcccgg tcagctcttc ctcggctttg cgaaggtgtt
     1801 ttgagcggtg agacttcatc tcattcatga gcttgaagcc ttctttatcg gccagctcgc
     1861 cgcgctcaac cagcttgttg ataaaatcct cgatctcatc ctgggccaga gcgaccgcgc
     1921 caacggaggc cagcagcagc cgccgggtcg cctcatacag cgggcggtct cccttcatgt
     1981 gtggagaacc tctcggctcg cttgtttcgg ttgtcatttg atcagctcct tctcgcaggt
     2041 cagattatga cgcattgcgt tatgaaatta taccacactg cggcaaatga ataaagcctg
     2101 ttccgccgta tcttaccggc agagcaggct ttctggttat gactcgaaag tgaaatgttc
     2161 aggcgctggc gggcgcgaat gtatcgatca gcagttttac atcgccgccc accggataca
     2221 ggatggggca ggtgcagcca tgttttttgt attcttccac cttggcacgc gcctcatccg
     2281 gtgtgccaga ggcggtaatc ttataaatca gttcttcagg gaccaggtgc ttggctttct
     2341 gaatttgctc tctggtcgcc ggccagccca ggatcgactg gatctctgcg accacatcca
     2401 tcgaaacgcc ggaagccctg gcaatgtgcg gctgctgggc cagatattgg gtgagcagct
     2461 cgcgggtggt atcgatggcg cgctcgtgat cataatc
//
LOCUS       DATMXK010000042         7226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_157812, whole genome shotgun sequence.
ACCESSION   DATMXK010000042 DATMXK010000000
VERSION     DATMXK010000042.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7226
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157812"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1074
                     /locus_tag="VN363_00815"
     CDS             <1..1074
                     /locus_tag="VN363_00815"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWQ83071.1"
                     /translation="GPYGIVDDLYLRLVEHLNLGTPVGPFRSGHSISYMDDRLLERLG
                     TDIRYCWSGLLPNSPVQPGEDADTFYDSYGQVWKRALPYYYAGPGLLQETTSLDDIDR
                     LVRWPDLNDPRWTSGLAERARTLRENTGYFVTMRMVASHGPFQTACDLRGTENFLMDL
                     TLHPEFAQALLDKVTEAINGLLHLVMQAGGKWFDMIELPGDDYAGNASTIISPRMFRQ
                     FIKPCLARLITTIKECNPDIAIMLHSDGAITRLMPDIIDLGVDVIHPLEPLPATDIPA
                     VKANFGAQVTFLGGIDIAHAMPGTREDVIHEVKKRIIQLATGGGYILAPSNHLQADVP
                     PENVVTLFQTAREFSIQSNIGPD"
     gene            1107..1856
                     /locus_tag="VN363_00820"
     CDS             1107..1856
                     /locus_tag="VN363_00820"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWQ83072.1"
                     /translation="MSLPYAAVDPHSPVPLYYQIYLDLRQMIQREVIPPDGMLPPEME
                     ICQAYNVGRQTVRQAIARLVDEQFVERFAGRGTFVRAQPSHTQFFLDRSFTQQMRELG
                     RQPHSRLLRIETGVIDAGINPALNDWHGHACLTIERLRLGDNEPICQQSSTVLLDRCP
                     GIEQVDFSNQSLYEVLSTRYQLAVTRIDHVVRAVAADGYRADLLEVPEGTPLLFVDTR
                     AYTEGGGLIEHSASYYRADRYEYSTTQTYRA"
     gene            1907..2683
                     /locus_tag="VN363_00825"
     CDS             1907..2683
                     /locus_tag="VN363_00825"
                     /inference="COORDINATES: protein motif:HMM:NF002805.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWQ83073.1"
                     /translation="MPSSAFEYIHQAQTILARIAASQMEAIERAAEQCAQTISQYGLV
                     HMFGTGHSRIFVEEMFPRHGSFPGFHPIVELSLTFHNVVVGANGQRQAMFLEHVEGLG
                     QTILRNFVFSPADCFLIFSNSGVNEVVIDVALEAKRQGFPVIAVVSLDHCSRSPAPHS
                     SGKKLPDIADIVIDNCTPGGDALVHIAGLEDPVGPGSTIGSAAVTNAIKCLVAEKLVA
                     QGKPPIVLTSSVLIGSEASKQRFDECYDDYRARIRQIYGC"
     gene            2673..3455
                     /locus_tag="VN363_00830"
     CDS             2673..3455
                     /locus_tag="VN363_00830"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ83074.1"
                     /translation="MAADMHLEDKIAIITGGGSGIGQATARLFAAEGAQVILFEIDPG
                     AAAETLASLSGKHPGEAIQLDLTKPVEVAAGIQEVVTRYGRVDVMVNIAGGSGRKWGD
                     GPADACTLDGWQKTLDLNLNSQFYCCKYALQAMLPYKHGSIVNVSSILGVVGGDEDFA
                     THAYAASKGAIISLTRAIASYYAPRGIRANVILPGLIATPMSRRAQTSEQIRGRLAQL
                     QPLTGDFGQPMDVAQAALYLASDASSFVTGTTLTVDGGWTVR"
     gene            3504..4538
                     /locus_tag="VN363_00835"
     CDS             3504..4538
                     /locus_tag="VN363_00835"
                     /inference="COORDINATES: protein motif:HMM:NF013847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-independent methionine synthase II
                     family protein"
                     /protein_id="HWQ83075.1"
                     /translation="MKERPLRTTVIGSYAFPSWLEFASQHLDQFGTNEFTEIQEDAVI
                     AAIHDQLAAGLDVITDGEQSRLDFNLSFYGYLQGLAPVPRPLRVFGPPAHDQRGKHEI
                     VAELTAPHGLGVVEEFTRLKRLAPQGPTLKASVPGPYTLSGRLNPNQRYPDRYAITEA
                     LIPIVRAELGALIQAGCTEITLDEPSMSCYAYKEDPKHFVEIFNRTVEPVVGKCRLST
                     HLCFGNFKARAVGPRQYAPMFPDFLAMNVDEIHLEMASREFSELELIAEVARVKDVAV
                     GIVDVKSYYIETPQDIAGRVRRCLKYAPPERLAFAPDCGLSQTARWAARLKLSNLVAG
                     VNIVRRELGL"
     gene            4559..5413
                     /locus_tag="VN363_00840"
     CDS             4559..5413
                     /locus_tag="VN363_00840"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ83076.1"
                     /translation="MMQKPLLQDEAFLADVRAAHPDQLHLWWLGQSGYLVRWRDSHLL
                     IDPYLSDSLTQKYAGTSKPHVRMTERVISPERLDFIDVVTSSHNHTDHLDGDTLIPLL
                     GANPALNVVVSRANLAFAAERLQTPAERLTPIRAGGDPIQIGPFKFAAIPSAHETLET
                     DENGDHKFLGYLITVAGRTIYHSGDACLYEGLVERLKAWKIDLALLPINGRDPKRGVA
                     GNFSAAEAVQLGKDIQAGLVVPCHYAMFEFNTVSPAEFTHLAGTRQVPYQVLQCGERL
                     SLTAGAGS"
     gene            complement(5554..>7226)
                     /locus_tag="VN363_00845"
     CDS             complement(5554..>7226)
                     /locus_tag="VN363_00845"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="PDZ domain-containing protein"
                     /protein_id="HWQ83077.1"
                     /translation="HTLTITSLFVLATLLLSACSAVPAQQAEASNPVANPVAEANAQA
                     TGAPQLQSQTGDASGLLAAYEGALENVYIQVNPSVVNINVVEQQSTAGLDPGQFPFGG
                     PEGNLPDVPEYSQGLGSGFVWDTEGHIVTNNHVVAGADRIEVTFSDGTTLPATLVGTD
                     SYSDLAVLKVEDAGDLLRPVQVADSSLVKVGGLAIAIGNPYGLDGTMTLGIISALGRS
                     LPATDGNTFGPVYSIPNIIQTDAPINPGNSGGVLVDENGQVVGVTAAIESASSANAGI
                     GFVIPSNIVSKVVPSLIESGSYEHPYLGISGTDLTREMAAAMGLDAAQRGVLVISATE
                     DGPAAKAGLIASEDSTTVGGQNLPVGGDVITAIDGQPINTMDELIAYLNDQTEVGQTV
                     SITVLRDGSEMTLDITLAARPVSTPEPDLTAALPDPGTESGQLPRSGSSWLGILGAEL
                     TPEVAQAMDLPEDQQGVLVVQVESGSPADEANLQASDQEITLNGQTVMIGGDIITAVD
                     GIPLTSLDELRSLIQTYEPGSEVTVSLLRDGESIEVAVTFAARPSNIP"
BASE COUNT         1507 a         2203 c         1996 g         1520 t
ORIGIN      
        1 ggtccctatg gcatcgtcga tgatctctac ctgcgcctgg ttgagcacct taatctcggc
       61 actccggtgg ggcctttccg cagcggtcac agcatttcgt atatggatga tcgcctgctc
      121 gagcgcctcg gcaccgatat ccgctactgc tggtctggac tgctgcccaa cagcccggtg
      181 cagccaggcg aggacgccga taccttctat gattcctacg gtcaggtctg gaagcgcgcc
      241 ctgccctatt attacgccgg gcctggactc cttcaggaaa cgaccagcct ggatgatatc
      301 gaccgactcg tccgctggcc tgatcttaac gacccgcgct ggacgtctgg attggccgaa
      361 cgcgccagaa cgctgcgcga aaacacaggt tattttgtaa ccatgcgtat ggttgcttct
      421 cacggtccat tccagaccgc ctgcgacctg cgcggcaccg aaaacttcct gatggacctg
      481 accctgcacc ctgaatttgc ccaggcgctg ctggataaag taaccgaagc gatcaatggg
      541 ctgctgcacc tggtgatgca ggccggggga aaatggttcg atatgatcga gctgcccggc
      601 gacgattatg ccgggaacgc cagtaccatc atctcgccgc gcatgtttcg ccagtttatc
      661 aagccctgcc tggcccggtt aatcacgacc atcaaagaat gcaatccaga tatcgccatt
      721 atgctccaca gcgatggcgc aatcacccgc ctgatgccgg atatcatcga cctgggcgtc
      781 gatgtgatcc acccgctgga gcctctcccg gcaaccgata ttccagccgt aaaggcgaat
      841 tttggcgccc aggttacctt cctgggtgga attgatatcg cccacgccat gcccggcaca
      901 cgtgaggatg tcatccacga ggtcaaaaag aggattatcc agctggctac tggaggcggc
      961 tacatcctgg ccccctccaa tcaccttcag gcggatgtgc cgccggaaaa tgtcgtgaca
     1021 ctcttccaga ctgctcgcga attctcgatc cagagcaata ttggccctga ttagcctgat
     1081 tcaggccgcc tgaggcgccg aagcccatgt ccttaccgta cgctgccgtc gatccacaca
     1141 gtcccgtgcc gctgtattat caaatttacc tggatctgcg ccagatgatc cagcgggaag
     1201 tgattccacc ggatggcatg ctgccgccgg aaatggagat ttgccaggcc tacaacgtag
     1261 gccgccagac ggtccgccag gcaattgccc gtctggtaga tgagcagttc gtcgaacgct
     1321 ttgccgggcg agggaccttt gtccgtgccc agcccagcca cacccagttt ttccttgacc
     1381 ggagcttcac ccaacagatg cgcgagctgg gtcgccagcc ccactcacgc ctgctgcgaa
     1441 tagaaaccgg tgtcatcgac gccgggatta atccggctct caatgattgg cacggccatg
     1501 cctgtctcac gattgagcgc ttgcgcctgg gtgataacga gcccatctgc cagcagtcca
     1561 gcactgtcct gttggaccgc tgtcccggaa tcgagcaggt ggatttttct aaccaatcat
     1621 tatatgaagt cctctctacc cgctaccagc ttgccgtcac tcgcatcgac cacgtcgtgc
     1681 gcgccgtggc agccgatggc taccgcgccg acctgctgga agttcccgaa gggacaccgc
     1741 tgcttttcgt cgatacccgc gcctataccg agggcggcgg tctcatcgaa cattcggcaa
     1801 gttattaccg cgccgatcgc tatgaataca gcacgaccca gacatatcgt gcataaaccg
     1861 gcgtattgct gaccggattc atcttgagat ctcccctgga ggcccgatgc cttcttccgc
     1921 cttcgagtac attcaccagg cccaaacgat cctcgctcgc atcgcagcgt cccagatgga
     1981 ggccattgaa cgcgcggctg agcagtgcgc ccaaacgatc agccagtatg gtctggtgca
     2041 catgtttggg accgggcact cacggatatt cgtcgaagaa atgttccccc gccacggcag
     2101 ctttcccggt ttccacccca tcgttgagct ctcgctgacc tttcataatg tagtcgtcgg
     2161 cgctaacggg cagcgccagg cgatgttcct ggagcatgtc gaaggtctgg gccagaccat
     2221 cttacgcaac tttgtctttt caccggccga ttgttttctt attttctcga acagcggggt
     2281 caacgaggtg gtcatcgatg ttgccctcga ggctaaacgc cagggtttcc cggtgatcgc
     2341 cgtcgtctcc ctcgatcact gttctcgctc accggcaccc cactctagcg gcaagaaatt
     2401 acctgatatc gccgatatcg tgatcgacaa ctgcaccccc ggcggggatg cactggtaca
     2461 tatcgccggg ctggaagacc cggtgggtcc cggctcaacc atcggatcag ccgcggtcac
     2521 caacgctatt aagtgcctgg tcgccgaaaa gctggtcgcc cagggaaaac cgcccatcgt
     2581 gctgaccagc tcggttttga tcggcagcga agcttccaaa cagcgctttg atgaatgcta
     2641 tgatgattac cgggcacgga tacgccagat ctatggctgc tgacatgcac ctcgaggata
     2701 aaatagccat tatcacgggg ggaggcagcg ggatcggaca ggccactgcc cggctgttcg
     2761 cggctgaagg ggcgcaggtg atccttttcg agatcgaccc cggtgcagcc gccgagactc
     2821 ttgcctcgct aagcggaaaa catcccggtg aggccatcca gcttgatctt accaaacctg
     2881 ttgaagtcgc cgccggaatt caggaagttg tgacccggta cggtcgtgtg gatgtaatgg
     2941 tcaatatcgc cggcggctcc gggcggaaat ggggcgatgg gccagccgac gcctgcacgc
     3001 tggatggctg gcagaagacc ctcgatctta acctgaacag ccagttttac tgctgcaaat
     3061 acgcattaca ggccatgctt ccctacaaac acggctctat cgtgaatgtt tcttctattc
     3121 tgggggtcgt cggtggggac gaagattttg ccacacatgc ttacgccgcc agtaaaggcg
     3181 ccattatcag cctgacccgc gccatcgcat cctattacgc cccgcgcggg attcgcgcca
     3241 atgtgatctt acccggcctg attgccacgc ccatgagccg gcgcgcccag accagcgagc
     3301 agatccgcgg ccgcctggcg cagctccagc cgctgaccgg cgattttggc cagccaatgg
     3361 atgttgccca ggctgctctt tacctggctt cagatgcctc cagctttgtg accggtacca
     3421 ccctgaccgt cgatggtggc tggaccgtcc gctaaatcag caataaacga ccagcaggct
     3481 ggtcagattt tcgaggagac ccgatgaaag aacgtccttt acgcaccacc gtgatcggca
     3541 gctacgcctt cccctcctgg ctcgagtttg ccagccagca cctggaccaa ttcggtacca
     3601 acgagttcac agaaatccag gaagacgcgg tcatcgccgc catccatgac cagctggctg
     3661 ctgggctgga tgtgatcacc gatggtgagc agtcacggct cgattttaac ctgtcatttt
     3721 atggctatct gcagggcctt gcgcctgtcc cgaggccgct gcgtgtgttt ggaccgccag
     3781 cccatgatca gcgcggcaag catgaaattg ttgccgagct gaccgcccca cacggcctgg
     3841 gagtggtgga agaattcaca cgcttgaagc gcctggcgcc tcaggggcca accttgaaag
     3901 ccagtgtccc gggaccttac accttgagcg ggcgcctgaa cccaaaccaa cgctatcccg
     3961 accgctatgc catcacggaa gccctgatcc ccatcgtacg cgccgagctg ggagctttga
     4021 tccaggcggg ctgtacggag atcaccctgg acgagccttc gatgagctgt tacgcctata
     4081 aagaagaccc aaaacacttt gtcgagatct tcaaccgcac agtggagccg gtcgtgggca
     4141 aatgccgcct ctccacgcac ctgtgttttg gcaatttcaa agcccgcgcg gttggaccgc
     4201 gccagtatgc gccaatgttc cccgatttcc tggcgatgaa tgtcgatgag atccaccttg
     4261 aaatggccag ccgggaattt tcagagctgg agctgatcgc agaggtagcc cgggtcaaag
     4321 acgtcgcagt tgggatcgtg gacgttaaaa gttactacat tgagaccccc caggatatcg
     4381 ccgggcgggt gcggcgctgc ctgaaatacg ctccacccga gcgcctggcc tttgcccctg
     4441 actgcggctt gagccagact gcgcgctggg cggccaggct taaattgagc aatctggtcg
     4501 ccggtgtgaa cattgtgcgc cgcgagctgg gtctgtagcc aaccggctgc tggagaaaat
     4561 gatgcagaaa cctctgctcc aagatgaagc tttcctggca gacgtgcgcg ccgcccaccc
     4621 ggaccagctg cacctgtggt ggctgggcca gagtgggtat ctggtcagat ggagagattc
     4681 ccacctgctg atcgacccct atctttcaga ttcactcacc caaaaatacg ccgggacaag
     4741 caaaccccac gtgcggatga ccgagagggt catctccccc gagcggctcg acttcatcga
     4801 tgttgtgacc tcctctcaca atcacaccga tcatctcgac ggagacacat tgatccccct
     4861 gctgggcgcc aaccctgcgc tgaatgtggt cgtctcacgg gccaatctcg ccttcgccgc
     4921 agagcggctg cagacgccgg ctgagcgcct gacaccgatc cgcgctggcg gagatcccat
     4981 ccagattggc ccgtttaaat tcgcggcgat cccctctgcc cacgagaccc tcgaaaccga
     5041 tgagaatggc gatcataaat ttctgggcta tctcatcacc gtcgctggac ggacgatcta
     5101 tcacagcggt gacgcctgtc tctatgaagg gctggtagaa cgcctgaaag cctggaagat
     5161 cgacctggct ttactgccga tcaacgggcg tgacccgaag cgcggcgtgg ccgggaattt
     5221 tagcgctgcg gaagccgtcc aattgggcaa agatatccag gcaggcctgg ttgtcccctg
     5281 ccattacgcg atgttcgaat tcaacacggt ctcgcccgcc gaatttactc acctggccgg
     5341 aacccggcag gttccctatc aggttttgca gtgtggtgaa cgcttatcac ttacggctgg
     5401 agctggcagc taaaacaggt cggagccagg gaaaaactgc cgtaccaatc cgggttgtgg
     5461 tatccaccgc cctgattggt atgcaataca catcagaccc ggcagagatt atgccgggtc
     5521 tggtgtgtag ataacaataa tctaatagat cgttcagggg atattgctgg ggcgggcagc
     5581 gaatgtcact gccacttcga tcgattcgcc atcacgcagg agagacacgg taacttcgct
     5641 tcccggttca taagtctgga tcaggctgcg cagctcatcg agcgaggtca gaggtattcc
     5701 gtctaccgcg gtgatgatat ctccgccgat cataaccgtc tgcccattga gggttatttc
     5761 ctgatcgctg gcctgcaaat tcgcctcgtc agccgggcta ccgctctcaa cctggacaac
     5821 caggacaccc tgctggtcct caggaagatc catggcctga gcgacctccg gggtaagctc
     5881 agcgcccagg atacccaacc aggagctgcc tgaccgtggg agctgcccgg attcggttcc
     5941 agggtctggt aaagccgcgg taagatccgg ttctggggta gagaccgggc gggcagccag
     6001 ggttatatcc agggtcattt cactgccgtc ccgcagcacc gtgatgctga cagtctggcc
     6061 tacttcggtc tggtcgttaa ggtaggcgat caattcatcc atcgtattaa tcggctggcc
     6121 atcgattgca gtgatcacat cgccgccgac cggcaaattc tgaccaccca cagtcgtaga
     6181 atcctcgctg gcaatcaagc cggctttagc tgccgggcca tcttcggtgg cggatataac
     6241 cagcacaccc cgttgagcag catctaagcc catcgcagca gccatctcgc gggtcaggtc
     6301 agtgccgctg attccgaggt atgggtgctc atagctgccg gattcgatca gggatggcac
     6361 tactttactg acgatattgg atggaatgac aaagccgatc cctgcattgg cgctcgaggc
     6421 cgattcgatt gccgcggtaa cacctacgac ctgtccattt tcatcaacca gtacaccccc
     6481 ggagttgccc gggttgatgg gtgcgtcggt ctggataata ttggggattg agtaaaccgg
     6541 tccaaaggta ttgccatcgg tcgccggtaa tgaacgccct aaagcactga taatgcctaa
     6601 agtcatcgtc ccatccagac cgtagggatt accaatcgca atggccagac cacccacttt
     6661 taccaggctg gaatcggcca cctggacggg tcggagcaga tcgccggcat cttctacctt
     6721 aagaacagcc agatcactgt acgagtctgt tcctaccaga gtagcgggca gtgtcgtacc
     6781 atccgagaag gtcacctcaa tccgatccgc gcctgcgacg acatggttat tggtcacaat
     6841 atgaccctcg gtgtcccata cgaaacccga gccaagaccc tggctgtact ctgggacatc
     6901 cgggaggttt ccctctggcc cgccaaaggg gaactgtccg ggatccagac ctgccgtact
     6961 ctgctgctcg acgacattga tgttgaccac agatgggttt acctggatgt agacattctc
     7021 aagcgcgccc tcataggctg ccagtaaacc gctggcatcg ccagtctgcg attgtaactg
     7081 tggagcacca gtagcctgcg cattcgcctc ggcgacagga ttggccacgg gattgctggc
     7141 ctccgcctgc tgtgcaggga cagcactaca ggcagaaagg agcagggtgg cgagaacaaa
     7201 tagacttgta attgtaaggg tatgtt
//
LOCUS       DATMXK010000043         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_32503, whole genome shotgun sequence.
ACCESSION   DATMXK010000043 DATMXK010000000
VERSION     DATMXK010000043.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32503"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="VN363_00850"
     CDS             <1..1595
                     /locus_tag="VN363_00850"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ83078.1"
                     /translation="LEALARWNGRGQTIFKPGSDSSMALDSTFEALPAQPDDIAARIW
                     LLGSLHEVTAAGRAAQTYPRHPLVWSRQAAILAESNAHQALSALNRALDNWSSQSIEY
                     RYEYPIQLALQARLAYETGEGELAASAIQMALSHWPDEPRWHALCAEIELDQALQTQS
                     NYAQREAGIASAINHFEQAAELEPEEHSHALKLGQVYLWRGAPIQAIRALERATQIAP
                     NEAETWFSLAQAYRTSGDMDDARDCAEKALEKTSDPTAVQLLLAEIDLDTNETRAAHT
                     RILAILEAQPEHPKALHILALTLEKLNRSSEALATFDRLIPLSQAPFALELERARVLE
                     KVQGAKASIEALTQIVERYPAEPLALAGMARALLNDGQVQAAVEAAQKALAYNNGQLD
                     QIALADLNQRIGHHALQAGQLDLALHHLTLAVELDPNSLDAYLDLGSTYMERRQHNYA
                     LQIFNQAIALTHEDYRPYYHAGLALKELKDYQAAEEMLNRASQLAPGEIGIHRMLTAV
                     VALNLIHNRRTSNITHYPEGLK"
     gene            1592..>2449
                     /locus_tag="VN363_00855"
     CDS             1592..>2449
                     /locus_tag="VN363_00855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83079.1"
                     /translation="MITSSTAVSHPIEREQFLKILETAISVREYRYAREAALAWLAVY
                     PGDLTVNLMYAQVMLGNQRAKPVIPVLEQICQADPENLPALESYYQAQLLLKQPIKSD
                     LKGSLLALGSSLVKREKDSAWGAQLAMVRASLPKGEPEKAMELFQNVLGSRQHIPLVG
                     VTHLQILESLPDTPAIIKRKLGAFYHEKWPDCLQATLYLADWLMDGGEFDQAVGLLHE
                     AAGKDISAQVVERMWGVNHPYQSLWPEAIEATVEIPVPAAVAAVFGWNQIPARLPAHP
                     QSTAETQKTR"
BASE COUNT          554 a          754 c          707 g          434 t
ORIGIN      
        1 gcctggaagc actggcgcgc tggaacggcc gcggccagac gatcttcaag ccggggagcg
       61 acagcagcat ggcgctggat tctaccttcg aagctttgcc tgcccagccg gatgatattg
      121 ccgcccgcat ctggctgctg ggctcgctgc atgaggtgac ggccgcaggc cgggcggctc
      181 agacttaccc tcgccacccg ctggtctggt cacgccaggc cgcgatcctg gccgagagca
      241 acgcgcacca ggcgctcagc gccctcaacc gggcgctgga taactggagc agccagtcta
      301 tagaatacag atatgaatat cccatccagc ttgcgctgca ggcccggctg gcttacgaaa
      361 cgggtgaggg cgagctggcc gccagcgcca tccagatggc cttaagccac tggcctgacg
      421 agcctcgctg gcatgcgctg tgcgctgaaa ttgagctgga tcaagcctta cagacccaga
      481 gcaactatgc ccagcgggaa gcagggattg cctcggcaat caatcatttc gaacaggcag
      541 ccgagctgga acccgaagaa cactcgcatg ccttgaagct tgggcaggtc tacctgtggc
      601 ggggtgcgcc gatccaggca atccgtgccc tggaacgggc aacccagatt gcacccaacg
      661 aagcggagac ctggttctca ctggcccagg cttaccggac ctcgggagac atggacgatg
      721 cccgggactg cgctgagaag gcactggaaa aaaccagcga cccgactgcg gtccagctgc
      781 tgctggcgga aatagatctg gataccaacg aaacccgggc agctcatacc cgcatcctgg
      841 ccatcctgga agctcaacca gagcatccga aagccctgca tattctggcc ctgaccctgg
      901 aaaagctgaa tcgctcgagt gaagccctgg ctacatttga tagactcatc ccgctgagcc
      961 aggcgccatt tgccctggag ctggagaggg cgcgtgtcct ggagaaagtt cagggggcga
     1021 aagccagcat cgaagctctc acgcagatcg tggagcgcta tcctgctgag ccgctggctc
     1081 tggccgggat ggcacgggca ctgttgaacg acgggcaggt gcaggcggca gtcgaagctg
     1141 cccaaaaagc gctcgcttac aataatggtc aactggacca gattgccctg gccgatctca
     1201 accagcgcat cggtcaccat gccctgcagg ccggacagct tgatctggcc ctgcaccacc
     1261 tgaccctggc agtcgagctt gatcccaaca gcctggatgc ctacctggac ttgggatcca
     1321 cctacatgga gcgccgccag cataattacg ccctgcagat cttcaaccag gcgattgccc
     1381 tgacccatga ggattaccgc ccttattatc atgccggatt ggccttaaaa gagctgaaag
     1441 actaccaggc agccgaagag atgctcaacc gtgcatcgca gctggcaccc ggtgaaatcg
     1501 gtattcaccg catgttgacc gcagtcgtgg ccctgaacct gatccacaac cggcgcacgt
     1561 cgaatataac ccattatccg gaagggctaa aatgatcacc tcatccaccg cagtcagcca
     1621 ccccattgaa cgcgagcaat ttctaaagat cctggaaact gccatctcgg tgcgtgagta
     1681 ccgctatgct cgcgaagctg ctctggcctg gctggcagtc tatcccggcg acctgacggt
     1741 aaacctgatg tatgcccagg ttatgctggg taaccagcgc gccaaaccgg tgatcccggt
     1801 tctggagcag atctgccagg ccgatccaga gaaccttccg gcgctggaaa gctactatca
     1861 ggctcaatta ctgcttaaac agccgatcaa aagcgatctg aagggcagtt tgctggccct
     1921 gggcagcagc ctggtaaaac gcgaaaaaga ctccgcctgg ggagcgcagc tagctatggt
     1981 gcgggcgtcc ttacccaaag gcgagcctga aaaagccatg gagctgttcc agaatgttct
     2041 gggcagccgc cagcatattc cactcgttgg agttactcac ctgcagatcc tggaaagcct
     2101 gcccgataca cccgcgatca tcaaacgcaa attgggagcc ttttatcatg aaaagtggcc
     2161 ggactgcctg caggcgacgc tttacctggc agactggctg atggacggcg gtgaattcga
     2221 ccaggcagtg ggactgctac acgaagccgc cggaaaagat atctctgccc aggtcgtgga
     2281 acggatgtgg ggggtcaatc atccctacca gtcgctgtgg cccgaagcga ttgaagcgac
     2341 ggttgagatc cccgtcccgg ccgctgtagc ggctgtattc ggctggaacc agatccctgc
     2401 caggctgccc gcccaccccc agtctacagc cgaaacccaa aagacgaga
//
LOCUS       DATMXK010000044         4061 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_20383, whole genome shotgun sequence.
ACCESSION   DATMXK010000044 DATMXK010000000
VERSION     DATMXK010000044.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4061)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4061)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4061
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20383"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..973)
                     /locus_tag="VN363_00860"
     CDS             complement(<1..973)
                     /locus_tag="VN363_00860"
                     /inference="COORDINATES: protein motif:HMM:NF014081.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ83080.1"
                     /translation="MSLKLPGLVDVHVHMREPGAEHKEDWDSGTAAALAGGFTMVLAM
                     PNTRAPVTDADSLELALEAARRKARCDYAQFLGAGPDNLDETPSLASRVAGLKMYLDQ
                     TYGPLRLDEMSLWQAHFERWPVRSAGPVVVHAEGRTLAAAILWAGLCQRPLHVAHVAT
                     AEEILLIRKAKERGLAVTCEVTPHHLFLTQEDLPRLGGGSSGRGEVRPRLAAPADRDA
                     LWDNLEVIDCFATDHAPHTAAEKDSENPPPGFPGLETALALLLEGVHDGRMSLEDLVA
                     RLHTNPRRIFGLPEQPDTYIEVDPDKVWEIRAAESHTRCGWTPFEGRQ"
     gene            complement(970..1122)
                     /locus_tag="VN363_00865"
     CDS             complement(970..1122)
                     /locus_tag="VN363_00865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83081.1"
                     /translation="MPKIIGYLPNDTPPIGQIILPGFQHALTMFPATRLARKLPVKPS
                     YLERLK"
     gene            complement(1300..2067)
                     /gene="surE"
                     /locus_tag="VN363_00870"
     CDS             complement(1300..2067)
                     /gene="surE"
                     /locus_tag="VN363_00870"
                     /EC_number="3.1.3.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558988.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008252 - nucleotidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="5'/3'-nucleotidase SurE"
                     /protein_id="HWQ83082.1"
                     /translation="MHILVTNDDGVTAPGLLALAQEMRQLGDVTVLAPDRNWSASGHV
                     KTLDRPLRVKEVVLADGSQALATDGAPSDCVALPVLGLLTEKIDLVVSGINPNANVGH
                     DVTYSGTVTAAMEAAIWGLPGIAVSLDRPAELLERVDYGPAARLAKRIVAQVIARGLP
                     KDILLNVNVPYRSDDELREVRITRQGLRVYHDLLDARIDPRGRPYYWIGGEAPTGVPE
                     DGTDFGALAEGTVSITPLHLDLTAHHWMERLRSWSWE"
     gene            complement(2159..3154)
                     /gene="yjjX"
                     /locus_tag="VN363_00875"
     CDS             complement(2159..3154)
                     /gene="yjjX"
                     /locus_tag="VN363_00875"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR00258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0046128 - purine ribonucleoside metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="inosine/xanthosine triphosphatase"
                     /protein_id="HWQ83083.1"
                     /translation="MPVIAIASHNPVKIEAARQGFQSAFPEQAFTLIPVTADSGVSAQ
                     PFSDQETLAGAEERATQASLALPDADYWVGIEGGVEEHPCGMAAFAWVVARGAVRGAA
                     LTGRGRSAAFFLPEAVAALVRQGMELGAADDLVFGRSNSKQANGAIGLLTGDVIDRAG
                     LYVPAVVMALIPFKNPELYPQETSAPEDGILISSDPARLDLEAIHGYLSQYSYWAKDI
                     PRGRVARSLANSLCIGAYQNGQQVGLVRVITDRATYAYLTDVYVVEALRGRGIARRML
                     AAAQAHPDLQGLRRWGLITRDAHDLYLPFGFTPLANPERHLEIYNPESYPARDEA"
     gene            complement(3166..3960)
                     /locus_tag="VN363_00880"
     CDS             complement(3166..3960)
                     /locus_tag="VN363_00880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560645.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ83084.1"
                     /translation="MDLKLKDLRALVCGSSRGLGYATALCLAAEGARVVVNSRKPDGA
                     AQAAESIRQQTGNVQVHAIAGDVADPQVPAELVSQAAAVLGGLDILITNSGGPTAGSF
                     ASLDDTAWQAAVELNLLSHVRLIRAALPYLEQSPAASVLTVTSYSVKQPIPGLVLSNS
                     VRAATVGLTKTLSLELGPLGIRVNSILPGWTETERVTVLMSARAAQNQTSIEEEIQKQ
                     AAASALGRLATPEEFANAAVFLVSPAAAYITGVMLTVDGGTVKGTL"
BASE COUNT          717 a         1315 c         1265 g          764 t
ORIGIN      
        1 cctggcgccc ttcaaaaggc gtccagccgc agcgggtgtg gctctcggcg gcgcggatct
       61 cccagacctt atcgggatcg acctcgatgt aggtgtcggg ctgttccggc aggccgaaga
      121 tgcgccgcgg gttggtatgc aggcgcgcca ccagatcctc caggctcatc cgcccatcgt
      181 gcacgccctc cagcagcaga gccagggcag tttccaggcc gggaaagccg ggcggcggat
      241 tctcgctgtc tttttcggcc gcagtgtgcg gggcatggtc ggtggcaaag caatcgatca
      301 cctccaggtt gtcccacagg gcgtcacggt cggcgggggc tgccaggcgt gggcgcacct
      361 caccgcgccc tgagcttcca cccccgaggc ggggcaggtc ttcctgggtg aggaacagat
      421 ggtgaggagt gacctcacag gtcaccgcca ggccgcgctc tttcgctttg cgaatcagca
      481 ggatctcttc tgccgtcgcg acgtgggcca cgtgcagcgg tcgctggcac aatcccgccc
      541 acaggatcgc cgctgccagg gtgcgcccct cggcatgcac gacgaccgga ccagcgctcc
      601 gtacgggcca gcgctcgaaa tgcgcctgcc acaggctcat ctcatccagg cgcagggggc
      661 cgtaggtctg gtcgagatac atctttagcc cggccacccg gctcgccagg ctgggtgtct
      721 cgtccagatt atctggccct gcaccgagga actgggcgta atcgcagcga gctttgcgcc
      781 gcgctgcttc cagagccagc tccaggctgt ccgcatcggt caccggggca cgggtgttgg
      841 gcatcgccag caccatggta aaaccacccg ccagggcggc cgctgtgccg gaatcccaat
      901 cctctttatg ctccgcgcca ggctcgcgca tatgcacgtg cacatcaacc agtccgggga
      961 gtttcaggct catttcaggc gctccaggta tgagggttta accggcaact ttctcgccag
     1021 cctggtagcc gggaacatgg tcagggcgtg ctggaagcca ggcaggatga tctggccaat
     1081 gggaggcgta tcgttgggta agtatccgat gattttgggc aaaaaaatag agccttgcgg
     1141 ctctacgctg gggataaaaa aatcgcccac ttgagcttgc gctccggggc gatccagtat
     1201 gcagggggaa acgggatatt tgcttgacat tccgaccgat tttacgccga ttcgagaatc
     1261 tgtcaagagg aattttggag gaaattatcc gcaaattgcc tactcccagc tccagctgcg
     1321 cagcctttcc atccagtgat gggcggtgag atcgaggtgc agcggggtga tcgagacagt
     1381 tccttctgcc agcgccccaa aatcggtgcc atcttcgggt acgccggtgg gcgcttcgcc
     1441 gccgatccag taataaggcc ggccgcgtgg gtctatgcgg gcgtccagca agtcgtggta
     1501 cacgcgcagc ccctggcggg tgatccgcac ttcgcgcagc tcatcgtcag accggtaggg
     1561 cacgttgaca ttcaacagga tatcttttgg caggccgcgg gcgatcacct gcgcaacgat
     1621 ccgtttcgcc aggcgcgctg ccggcccata atccacccgc tcgaggagct cagccgggcg
     1681 gtcgagcgag acggcgatcc cgggcaggcc ccagatggcg gcttccatcg ccgccgtcac
     1741 cgtgccggaa taggtcacat catgccccac attggcgttg gggttgatcc ccgataccac
     1801 caggtcgatc ttttccgtca gcaagcccag caccggcagc gccacgcagt ccgagggcgc
     1861 cccatcggtg gccagcgcct ggctgccgtc tgccagcacc acctctttga cccgcagcgg
     1921 acggtccagg gtcttgacat gcccggaggc cgaccagttg cggtcaggcg ccaggacggt
     1981 aacatcccct aattggcgca tctcctgggc cagcgccagc aggcccggcg cggtcacgcc
     2041 atcatcattg gttaccagaa tatgcatcag ttttccttcg cttggagaat aacccggcaa
     2101 ttgtatcagc cctgccgtcg tgacgagttc ttttaaatgc cggtcaggct gccgcggctc
     2161 aggcttcgtc tcgcgccgga tagctttccg ggttgtagat ttccaggtgc cgttccgggt
     2221 tcgccagcgg ggtaaagccg aacggcaggt acaggtcgtg ggcgtcgcgg gtgatcaatc
     2281 cccagcggcg caggccctgc aggtcggggt gagcctgggc ggccgccagc atgcgcctgg
     2341 caatcccccg cccgcgcagc gcctccacca catagacatc cgtcaggtag gcgtaggtcg
     2401 cccggtcggt gatcacccgc accagcccca cctgctgccc gttttgatat gcgccgatgc
     2461 acagcgaatt cgccagcgag cgggccaccc gtccgcgcgg gatatccttc gcccagtatg
     2521 agtactggct caggtagccg tgaatcgcct ccaggtccag ccgggccgga tcgctgctga
     2581 tcaggatccc atcctccggt gcggaggttt cttgcgggta cagctctgga ttcttgaacg
     2641 ggatcagcgc catgaccaca gcaggtacat acaggccagc ccggtcgatc acgtccccgg
     2701 tcagcaggcc gatagccccg ttggcctgct tggagttgct gcgcccgaag acgagatcgt
     2761 cggccgcgcc cagctccatg ccctggcgca ccagcgccgc caccgcttcg ggcaggaaga
     2821 aagccgcgct gcgcccacgc ccggtcaggg ctgcgccgcg caccgctccc cgcgccacca
     2881 cccaggcgaa ggctgccatc ccgcagggat gctcttccac accgccttcg atccccaccc
     2941 agtaatcagc gtctggcagc gccagacttg cctgggtggc gcgttcttcc gccccggcaa
     3001 gggtttcctg gtcactgaaa ggctgcgcac tgactcccga gtccgcagtg accgggatca
     3061 gggtgaaggc ctgctcgggg aaggcgcttt gaaagccctg gcgggcggct tcaatcttga
     3121 ccggattgtg ggaagcaatg gcgatgactg gcactgtaaa cgctcctaaa gggtaccttt
     3181 gaccgtccca ccatctacgg tcagcattac cccggtgatg tatgccgcag ccggcgagac
     3241 caggaaaaca gccgcgttgg caaattcttc gggggttgcc aggcggccca gggcgctggc
     3301 agcggcttgc ttctggatct cttcttcgat gctggtctgg ttctgggcgg cgcgggcgct
     3361 cattaaaacc gtcacccgct cggtctccgt ccagcctggc aggatcgagt taacccggat
     3421 gcccagcgga cccagctcca gagacagggt tttggtcagg cctacggtgg cggcgcgcac
     3481 actgttcgaa agcaccaggc cagggatggg ctgcttaacc gaataggaag tcaccgtcaa
     3541 tacgctggcg gccggggatt gttctaaata aggcaaagcc gcccggatca ggcgcacgtg
     3601 actgagtaga tttagctcca ctgccgcctg ccaggcggta tcgtccaggc tggcaaaact
     3661 gcctgcagtt ggtccaccag aattggtgat caggatatcc agaccaccca gaacggcggc
     3721 agcctggcta accagctccg ctggcacctg cgggtcggcg acatccccgg cgatggcgtg
     3781 gacctgtacg ttgcctgtct gctggcggat gctctcagct gcctgggcgg cgccgtccgg
     3841 cttgcggctg ttgaccacca cccgggcgcc ttctgccgcc aggcataggg ctgtggcgta
     3901 acccaggccg cggctcgaac cacataccag ggcgcgtaaa tcttttaatt ttagatccaa
     3961 ttctgcctcc ctttgatcct tacatttata agatctcgat tttttcgtcg accaggctga
     4021 aatcggggaa gttgacctcg atccaatggg gaatccgctg c
//
LOCUS       DATMXK010000045         2372 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_101982, whole genome shotgun sequence.
ACCESSION   DATMXK010000045 DATMXK010000000
VERSION     DATMXK010000045.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2372)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2372)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2372
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101982"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2359
                     /gene="mutS"
                     /locus_tag="VN363_00885"
     CDS             <1..2359
                     /gene="mutS"
                     /locus_tag="VN363_00885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0030983 - mismatched DNA binding [Evidence
                     IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA mismatch repair protein MutS"
                     /protein_id="HWQ83085.1"
                     /translation="DKGYHVAICEQIGEVPAKGLVTRQVTRVVTPGTIVEPALLPGDA
                     NNYLACFVLQSEMEGEESRSAGVAYVDITTGEFAATELTSRDILSSLRAELNRLNPAE
                     TLHPAAQTVNNGLPGHSTSWPDWRFEQGRCQEALLAHFEAASLDGFGLRGQSLAVRAS
                     GAILQYLKETQPAALKMLNRLSTYSLSDFMTLDASTRRNLELTETLRGGQVKGSLLGV
                     LDQTVSPMGKRQMSQWVSKPLLDVERIAARQAGVRYFFENGLLRAELRASLKPLNDLE
                     RLANRVASGIALPRDLVALRATLERLPGLRAIFPPEATPLDLVLEEYDPCTNELELLE
                     RALADEPPGTLATVGVIRPGYSAELDGVVERSRHAREWIANLEASERQRTGIKTLKVG
                     YNKVFGYYIELTRANSEMAPDDYIRKQTLVNAERYITPEMKEYEALVLNAEEQLREIE
                     GRLFRGLLVELDKSTPRLLKTARSLASLDVLASLADVAARGGYVCPQVVAEDVLEVRG
                     ARHPVVELTLSGERFVPNDIIFEPGERIRVLTGPNMSGKSTFLRQAALIVLLAQLGSF
                     VPAASARIGLVDRIFTRIGAQDEIHAGQSTFMVEMVETANILHHATPRSLLILDEIGR
                     GTSTYDGVSIAWAVVEYLHNHPGLRARTLFATHYHELTQLADLLPGVTNYNVAVSEAD
                     GKVVFLHKIIPGGADRSYGIHVGQLAGLPRPVVQRASEILRQLEATSGKAGKINPLAA
                     RQIALFPETNPLLDELKNLDVNGLSPIEALNKLYEWQKRFLDHDS"
BASE COUNT          435 a          720 c          740 g          477 t
ORIGIN      
        1 cgataaaggc tatcacgtgg cgatttgcga gcagatcggc gaggtgcccg ccaaaggcct
       61 ggtgacgcgc caggtgaccc gtgtggttac accgggcacc atcgttgagc ctgctctgct
      121 gcccggcgat gccaataatt acctggcttg ttttgtgctg cagtcagaga tggaaggtga
      181 agaatcgcgc agcgccggag ttgcctatgt ggacattacg accggtgaat ttgctgccac
      241 cgagctgact tcccgcgata tcctctccag cctgcgcgcc gagctgaatc gcctgaatcc
      301 cgccgagacc ctccacccgg cggctcagac ggtaaataat ggtctgccgg gtcactctac
      361 ttcctggccc gattggcgat ttgagcaggg tcgctgccag gaggctttac tggcccactt
      421 tgaagctgct tcgctggatg gctttggcct gcgcggccag tccctggcag tgcgcgccag
      481 cggggcgatc ttgcagtacc tcaaggagac ccagccggct gccctgaaga tgctcaaccg
      541 cctgagcact tacagcttat ccgactttat gaccctggat gcttccaccc gccgtaacct
      601 ggagctgacc gaaaccttgc gcggtggtca ggtaaaaggc tcgcttctgg gtgtgctcga
      661 ccagaccgtc agtccgatgg gaaagcgcca gatgagccag tgggtgagta agccgctcct
      721 cgatgtggag cggattgccg cccgccaggc aggtgtacgc tattttttcg agaatggcct
      781 gctgcgcgcg gagctgcggg cgtccctcaa accgctcaac gacctggagc gcctggcgaa
      841 tcgcgttgcc agtggaattg ccttgccgcg cgatctggtt gcgctgcggg ccacgctgga
      901 acgcctgccg gggctgcgcg ccatcttccc tcccgaagcg acccccctgg acctggtgct
      961 ggaggaatat gatccctgca ccaacgagct ggagctcctg gagcgggctc tggcggatga
     1021 gccgccgggt accctggcaa ctgtaggtgt gatccgcccg ggatattcgg ctgagctgga
     1081 tggcgtggtt gagcgttcgc ggcatgcccg cgagtggatt gccaacctgg aggccagcga
     1141 acgccagcgc accgggatta aaaccttgaa agtgggttac aacaaggtct ttggctacta
     1201 tattgaatta acccgcgcca acagcgagat ggctccggac gactatatcc gcaaacagac
     1261 cctggtcaac gccgagcgct acatcactcc tgagatgaag gaatatgagg ccctcgtcct
     1321 gaacgccgag gagcagctgc gggagattga aggccgcctg ttccgcggcc tgctggtgga
     1381 gctggacaaa tccacgccca ggctgctcaa gactgctcgc tccctggcta gcctggatgt
     1441 gctggcttcc ctggccgatg ttgccgcccg gggaggctat gtgtgcccgc aggtcgtggc
     1501 ggaagatgtt ctcgaagttc ggggggcccg ccatccggtg gtagagctta ccctgagcgg
     1561 cgagcgcttc gtccccaatg atattatctt cgagccgggc gagcgcatcc gcgtgctgac
     1621 cgggcccaat atgagcggaa agagcacctt cctgcgccag gctgccctga tcgtgctctt
     1681 ggcgcagttg ggcagtttcg tcccggcagc cagcgcccgg atcggcctgg tggaccgcat
     1741 cttcacccgg atcggggccc aggatgagat ccatgccggg cagtcgacct tcatggtcga
     1801 gatggtcgag actgccaaca ttctgcacca tgccacgccg cgctcgcttt tgatcctgga
     1861 tgagatcggc cgcggcacca gtacctatga tggggtttcg attgcctggg cggtggttga
     1921 atatttgcac aaccatccgg ggctgcgggc caggactctg tttgccaccc attaccacga
     1981 gctgacccag ctggcggatt tgctgcctgg cgtgacgaac tacaatgtgg ccgtgagcga
     2041 ggcggacggc aaggtggtat tccttcacaa gatcattccc ggtggggcag accgctctta
     2101 cggtatccat gtggggcagc tggctggctt gccccgccct gtggtgcagc gcgccagcga
     2161 gatcctgcgc cagctggagg ctacatccgg caaggcgggc aaaattaacc ccctggcagc
     2221 ccgccagatc gccctcttcc cggagacgaa tccactcctc gacgagctaa agaacctgga
     2281 tgtcaacggc ttatctccga ttgaggctct gaataaattg tacgagtggc agaaacgctt
     2341 cctggatcac gacagctgac cctgttattt cc
//
LOCUS       DATMXK010000046         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_44927, whole genome shotgun sequence.
ACCESSION   DATMXK010000046 DATMXK010000000
VERSION     DATMXK010000046.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44927"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VN363_00890"
     CDS             <1..311
                     /locus_tag="VN363_00890"
                     /inference="COORDINATES: protein motif:HMM:NF025607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HWQ83086.1"
                     /translation="TTATGLFALVKPSAIYGFTGLVAQGVRGISEIRAIFGGLFIALG
                     IAPFIFGPVGYQVLGLGYLAIAVVRLASILIDKSYASSNWISLAIEIVLGIILIWGVK
                     "
     gene            308..1153
                     /locus_tag="VN363_00895"
     CDS             308..1153
                     /locus_tag="VN363_00895"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ83087.1"
                     /translation="MSVHIVTDSTCDLPAATIADLGIQVLPLYIRVGDKDYLDGIDLT
                     REDFYRDLPGYKDYPTTAVPSPQKFRAMYDSLADQGASEVLSIHIATSLSATVNVATT
                     AAKETASIPVTVVDSRQLSMGTGFLVQTAAELARAGNTVSQILPRLQDQIKRTHVWAL
                     VDSLTYLRRSGRMSAVMSTIGELLQIKPILTMHDGVSKAEKVRTRKNGLARLIGRINE
                     LAPFEKIAFLHSNALQDAMDLKTTVQTLIPNQDTAFEIINPILGAHIGTGVVGFACVT
                     QDSRG"
     gene            1158..1937
                     /locus_tag="VN363_00900"
     CDS             1158..1937
                     /locus_tag="VN363_00900"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HWQ83088.1"
                     /translation="MTFFEMYALGLAAILALMIVLWFVSLLLKNSSIVDIFWGPGFVL
                     AAWTYFLLTPEGFLLRKLLITALVTIWGLRLAIHIFLRNKGKPEDFRYQKWREENGSS
                     WWWRSFFKVFLLQGILLWLISIPLLAAQIPALPARLTFFDYFGAVLWLIGFFFEAAGD
                     AQLARFKTDPANKGKIMTSGVWRFTRHPNYFGDAAQWWGYFLIALAAGGWWTIFSPIL
                     MTTLLLRVSGVALLEKTMSAKPGYEDYMRKTNAFVPWLPKK"
BASE COUNT          398 a          645 c          546 g          468 t
ORIGIN      
        1 ccaccactgc caccggtctt ttcgccctgg tgaaaccatc cgccatctac ggcttcaccg
       61 gtctggtagc ccagggagta cgcggcatct cagagatccg cgcgattttt ggcgggttgt
      121 ttatcgccct gggcattgca ccgtttatct tcggcccggt cggctatcag gtgctcggtc
      181 tgggttacct ggccatcgcc gtggtgcggt tggcatccat cctgatcgac aagtcctatg
      241 cctcttccaa ctggatcagc ctggcgatcg aaatcgtttt gggaatcata ctgatatggg
      301 gggtcaaatg agcgtccaca ttgtcaccga cagcacctgt gatttacctg ccgccaccat
      361 agccgacctg ggtatccagg tcctgcccct ctacatccgg gtgggcgaca aagattacct
      421 ggacggcatc gacctgaccc gcgaggattt ctaccgggat ttgcccggct acaaggacta
      481 ccccactacc gccgtcccct cgccgcagaa attccgcgcc atgtacgatt ccctggcgga
      541 ccagggcgcc agcgaagtcc tatcgattca tatcgccact tcgctcagcg ccacggtcaa
      601 cgtcgccacg actgccgcca aagaaaccgc ctccattccg gtgaccgtgg tcgattcccg
      661 tcagctgagc atgggcaccg gctttttggt gcagaccgcg gcggaactgg cccgcgccgg
      721 aaacacggtc agccagattc tgcccaggct gcaggatcaa atcaagcgca ctcacgtctg
      781 ggcgctggtc gatagcctca cctacctgcg ccgcagcggc cgcatgagcg ccgttatgtc
      841 caccatcggc gagctgctgc agatcaaacc catcctgacc atgcatgacg gcgtgtcgaa
      901 agccgaaaag gtacgcacgc gcaagaacgg cctggcccgg ctgattgggc gcatcaacga
      961 actggctccc ttcgagaaga tcgctttcct gcattccaac gcgctgcagg acgccatgga
     1021 tttgaaaacc actgtgcaga cgctgatccc caaccaggac accgccttcg agatcatcaa
     1081 cccgatcctc ggcgcacata tcggcaccgg cgtggtcggt tttgcctgcg ttacgcagga
     1141 ttcacggggg taatctcatg acattttttg aaatgtacgc gctcggtctg gctgccattc
     1201 tggcgctgat gattgtactg tggttcgtca gcctgctgtt gaagaactcc agcatcgtcg
     1261 atattttctg gggaccgggc tttgtgctgg ctgcctggac ctatttcctg ctcactccgg
     1321 aaggcttcct actccgcaag ctgctgatca ctgccctggt gaccatctgg gggctgcgcc
     1381 tggcgatcca tattttcctg cgcaataagg gcaaacccga ggacttccgc taccagaaat
     1441 ggcgcgaaga gaacggctct agctggtggt ggcgcagttt cttcaaggtc tttctgctgc
     1501 agggcatctt gctgtggctt atctccatcc ccctgctggc agcccagatc ccggcactgc
     1561 ccgcccgttt gaccttcttt gattatttcg gcgctgtcct ctggctgatc ggcttcttct
     1621 tcgaagccgc cggcgacgcc caattggcgc gcttcaaaac tgacccggct aacaagggca
     1681 agatcatgac cagcggcgtg tggcgcttca cccgccatcc caattacttt ggcgatgccg
     1741 cccagtggtg gggttatttc ctcatcgcct tagctgccgg cggctggtgg accatcttca
     1801 gccccattct catgaccacc ctgctgttac gcgtctccgg cgtggcgctg ctggagaaga
     1861 ccatgtccgc caagcccggc tatgaggact acatgcgcaa aactaacgcc ttcgtgccct
     1921 ggctgccgaa gaaatagtag gagaaggata tgagaagcta ttcttgacgg tcttaggata
     1981 tgtgcagatc gcgtatcata cccaattttc accattagga taaaaatttt tcaaataaca
     2041 attgccatgc ctttacc
//
LOCUS       DATMXK010000047         8360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_54729, whole genome shotgun sequence.
ACCESSION   DATMXK010000047 DATMXK010000000
VERSION     DATMXK010000047.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54729"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2583
                     /locus_tag="VN363_00905"
     CDS             <1..2583
                     /locus_tag="VN363_00905"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_019467715.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWQ83089.1"
                     /translation="MTCASCVSHVEGALKDLPGVDDVVVNLATNKATLNYDPQKVRLA
                     DMQRAVDDVGYAVPTSELTLDVSGMTCASCVDHVESALKELTGVQGAAVNLGLHTARV
                     TYIPGVVTASAMKRAVREAGYEATERSQGADALDRERQAREDEIKRQGRNLLISGIIG
                     LVVMIGTFYDMLGPLQAFVPKFLSYKWVIGLLTTPIVFGPGRQFFTNSWRGLKHGVTD
                     MNLLYATGIGASYGIAVINTILPKAGFGGEGATFFESAALLTTFIILGRWLEALTRGR
                     TSEAIRKLMKLQPKIARVIRHGKEEEIPAEEVEINDLILVRPGEGIPVDGIVADGYSA
                     VDESMLTGESLPVEKKAGDNVIGGTLNKTGAFKFTASRVGKDTALGQIIKLVEDAQAS
                     KAPIQKLADWVAGHFILGVHLLAVLVFIFWFFLGYQRFFDPGSSFMLSPYTLGEMGVF
                     GFSLLLSVTVLVISCPCAVGLATPSAMMAGTGKAAENGVLFKGAEGLEKASKLQTIVF
                     DKTGTLTKGEPSVTDVIADSRFQIEGSGVQSTINNQQSAILRYAAIAEKNSEHPLGEA
                     IVNGAASRGLSLVEAESFNSIPGHGIEAQVEGREILLGNRRLMREWEIDFSSLINQAE
                     ALEADGKTVMFVAVDAQPAGLIAVADTLKEFAVEAIQRLHKLGVETAMITGDNHRTAE
                     AIARQVGIDRVLAEVLPQDKAEEVKKLQASGKIVAMVGDGVNDAPALAQANVGMAIGS
                     GTDVAKETGDVILIKDDIRDVVVALEVARATMSKVKQNLFWAFFYNTLSIPLAAGLLY
                     PFTSLIISPEIAGLLMAVSSITVTLNTLLLKGFKPSFQRNDRPNRGTGASESHLTPVA
                     ASGD"
     gene            2605..2856
                     /locus_tag="VN363_00910"
     CDS             2605..2856
                     /locus_tag="VN363_00910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83090.1"
                     /translation="MENEQMEHEQIEKEIARRSALIYLPISLGAVLLFLLAASLVGGY
                     PPVARIGGTVWVGLLSLIVSMPIITSRVKRQVAMVRGNH"
     gene            2856..3230
                     /locus_tag="VN363_00915"
     CDS             2856..3230
                     /locus_tag="VN363_00915"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HWQ83091.1"
                     /translation="MITGSLITIREGLEAFLIIGILLGYLTKINQPQLKLHVWIGAGA
                     ALLVSILLAAVFQTLAIQFDGETAQLFEAGIALLAVAVLITCCSSVEKFFLARVAASP
                     AGEVMSIYSCSCLWRGLLCRRS"
     gene            3935..4747
                     /locus_tag="VN363_00920"
     CDS             3935..4747
                     /locus_tag="VN363_00920"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HWQ83092.1"
                     /translation="MNEIGPALTTITETTSGLSCLERWRYRFSRHWILIFSVILGLYA
                     GLPFLAPVFMRLGWEAPGRVIYLIYSFLCHQLPQRSFFLFGPNATYSLTEIQTIWQNT
                     ADPLILRQFMGNAEMGWKVAWSDRMVAMYISLWLFGILWRPLRSWLKPLPWWGFGLFL
                     LPMAIDGTSHFVSDLYGIGQGFRDSNAWLASLTINAFPRDFYAGDALGSFNSWMRLLT
                     GVLFGMGIVRFIFPYIDNTFSQQARKLESRLYYFQQKKLDVSISPSSGQQLP"
     gene            complement(4879..5232)
                     /locus_tag="VN363_00925"
     CDS             complement(4879..5232)
                     /locus_tag="VN363_00925"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83093.1"
                     /translation="MNSLQISNERLDVSKAAASLWIANAASTLTGLDAGVDPGYNRFG
                     SIGGEFCIDAPCCMPEAVAYKYVILSSVFLVHTLTQVGFQKGLCTILTVIYNRTVRRT
                     TKTILYRAYFSKWDV"
     gene            5235..5912
                     /locus_tag="VN363_00930"
     CDS             5235..5912
                     /locus_tag="VN363_00930"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83094.1"
                     /translation="MIPTATPIRVLLADDHNIVREGIRQVLEHASDIQVVAEASDGEV
                     AQDLLRQHHPDVAVLDIQMPKASGIEVTRWVRANMKDVGVLVLTAYDDDPYVMAVLQA
                     GANGYVLKTASPAELIQAVRDVHEGKSVIDASIAQKLMSHFFTGSKTTVVEQLTDREL
                     EVLSLTAKGYTNKAIGMQLGISDRTVQGHLAHIFDKLQASSRTEAVMRAVSLGWISQN
                     VGTIAEE"
     gene            5956..7656
                     /locus_tag="VN363_00935"
     CDS             5956..7656
                     /locus_tag="VN363_00935"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83095.1"
                     /translation="MPWRGLIWQLFVITILPLAVLTVVITFGSLTIHQQAMRALVGNR
                     DERAVRTAAAALEEQLSHRQDAIRSLALLAEKATPEEFSKAFSNFAYLRDDFDSGLAF
                     FSPEGLLESYTGDQQVWEGLVDQVGPMIRELLEQSNNPVLISSAYNNPANQEKIVLVL
                     AASSERNLVAAGASSAADLMRHTLTNSFASGSEASVVVVDANKDVLYQGGSFSYIGGA
                     AAHPGVAEALRGESGVTYVEVGDSEHVVTYSPIGSVGWALALEEPWETVDTPTLRTTQ
                     LAPLVLVPVLLLTLLALWFGLRSIVKPLQALESKASTLAWGEFDAIEQSVGGIAEIRR
                     LQYELIHMARKVKAAQQSLHSYIGAITSGQEEERRRLARELHDDTIQSLIALKRRVEL
                     AQLASKNGTSEASLVEVVSLTEKTIENVRRQTRALRPIYLEDLGLVTALEMLARETSQ
                     AANIPIDFQRQGTENRLNPNAELALYRMAQEALNNVIRHAQASQANLGISFTSQEVTL
                     YVSDNGRGFDVPKSPAEFAPGGHFGLLGMYERAELIGARLEIHSTPEQGSQITVRLPV
                     "
     gene            7784..8218
                     /locus_tag="VN363_00940"
     CDS             7784..8218
                     /locus_tag="VN363_00940"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWQ83096.1"
                     /translation="MTRITVIFLFLVASLLSACKSTAISTPAVDVVGQQVSIAGGSYT
                     DVSVDELQSMLANKDFTFVNVHIPFEGDIAGTDLSIAYDEIEQNLDQLPAEKDAKIVL
                     YCRSDRMSRMAAETLVGLGYTNLWNLDGGMVAWEKAGQPLER"
BASE COUNT         1939 a         2148 c         2244 g         2029 t
ORIGIN      
        1 atgacgtgcg cctcgtgcgt ctcccacgtg gaaggtgcac ttaaagatct ccctggtgtg
       61 gacgacgttg tcgtcaacct ggctaccaat aaagccacct tgaactatga tccccaaaag
      121 gtcaggctgg cagatatgca gcgtgctgtg gatgatgtgg ggtatgctgt cccaacttca
      181 gagcttactc tggacgtgag cggtatgacc tgcgcttctt gcgtggatca tgttgaaagc
      241 gcgctcaaag aattgaccgg tgtgcaagga gcagccgtta acctcggact gcacactgcc
      301 cgtgtgactt atatcccagg cgttgttact gcctctgcca tgaaacgtgc tgtgcgtgaa
      361 gccggttatg aggccaccga acgcagtcag ggcgcagacg ctctcgaccg cgaacggcag
      421 gctcgggaag atgaaattaa acgccagggt cgcaatctgc ttatttccgg gatcatcggc
      481 ctggtcgtca tgatcggtac gttctacgat atgctcggcc cattacaagc atttgtcccg
      541 aagtttcttt cttataagtg ggtcattggc ttgctgacca cccctatcgt cttcggaccc
      601 ggacggcagt tcttcaccaa ctcctggcgc ggcctgaagc atggggtcac agacatgaac
      661 ctgctctacg ccaccggcat tggcgcctcc tatggcattg ctgtgatcaa cacgatcctc
      721 cctaaagccg gctttggcgg cgaaggggct acattctttg agagcgcggc cctgctcacc
      781 actttcatca tcttaggacg ctggctggag gcgctcaccc gtggtcgcac ttcggaagcc
      841 atccgcaagt tgatgaagct tcagcccaag atcgcacgcg tgattcgcca cgggaaagaa
      901 gaggaaatcc ccgccgagga ggttgagatc aacgacctga ttctcgtccg tcctggcgag
      961 ggcatcccgg tggatgggat cgtagcagat ggctattcgg ctgttgacga atcgatgttg
     1021 accggagaaa gcctcccggt agagaaaaag gctggcgaca atgttatcgg cggcacattg
     1081 aacaagactg gcgcatttaa gttcacggct tcccgggtcg gcaaagatac agccttagga
     1141 cagatcatca agctggtaga agacgctcaa gccagcaaag cgcctatcca aaaattggct
     1201 gactgggtgg cgggacactt catcctcggt gtacatcttc tggctgtgct ggtatttatt
     1261 ttctggtttt tcttgggtta ccaacgcttc ttcgatccag gctcaagttt catgctctcg
     1321 ccgtataccc tcggtgaaat gggcgtcttt ggcttctcac tcctgctttc ggttacagtc
     1381 ctggtcatct cctgcccatg cgctgttggt ttggcaacac cgagcgccat gatggcaggt
     1441 acaggcaaag ctgctgaaaa cggtgtgtta ttcaaaggcg cagaaggctt ggagaaggcc
     1501 agtaagctgc aaaccattgt cttcgacaag acgggtacgc ttacaaaagg cgagccatcg
     1561 gtaaccgatg taattgcaga ttcgagattt cagattgaag gatcaggagt tcaatcgacg
     1621 atcaacaatc aacaatctgc aatcctccgc tacgcggcta ttgccgagaa gaacagcgaa
     1681 caccctcttg gggaggcaat tgtcaatggt gctgcatcgc gagggctctc gttggttgag
     1741 gcagaatcgt tcaactctat tccaggtcat ggtatcgagg cgcaggtgga aggtagagaa
     1801 atcctgctgg ggaatcgcag gctgatgcgg gaatgggaaa tagatttctc gagcctgata
     1861 aaccaggccg aagcattgga agccgacgga aaaactgtga tgttcgtcgc agtagatgct
     1921 cagcccgctg ggttgatcgc agtggcagat accttaaagg aattcgccgt tgaagccatc
     1981 caacgcctcc ataagctggg tgttgagaca gccatgatca ccggcgacaa ccaccgcaca
     2041 gccgaagcca tcgcccggca agtgggtatc gaccgcgttt tggctgaagt gttacctcag
     2101 gacaaggcag aagaggtcaa gaaactgcaa gcctcgggca agatcgttgc catggttggc
     2161 gatggtgtca acgacgcccc agctctggct caggcaaatg tgggcatggc tatcggctcg
     2221 ggtaccgacg tagccaaaga gaccggcgat gtaatcctga ttaaagatga catccgcgac
     2281 gtggtagtgg cgctggaagt cgccagggcg accatgtcta aggtaaagca aaacctgttt
     2341 tgggcctttt tctacaatac cctgagcatc cccctggctg caggtttgtt atacccattc
     2401 acctcgctga tcatcagccc ggagatagct ggacttttga tggcagtcag ctcgattact
     2461 gtgaccctta ataccctgct tttgaaagga tttaaacctt ctttccagcg caatgatcga
     2521 ccaaatcgtg gcacaggtgc atcagagtca catctgacgc cggtagctgc aagcggcgat
     2581 tagccgaatt taaggaaatc ttctatggaa aatgaacaaa tggaacacga acaaatcgaa
     2641 aaggaaatag cgcgtcgtag tgcgctgatc tatctaccca tttccttggg tgcagtcttg
     2701 ctgttcttat tggctgccag cctggtgggt ggttatcctc ccgtggccag gatcggaggt
     2761 acggtctggg ttggcttact gagtctgatt gtgagcatgc ccatcattac ctcgcgcgtc
     2821 aaacgccagg ttgcgatggt tagggggaac cattaatgat caccggctcg ctgatcacca
     2881 tccgtgaagg tctggaggcg ttcctgatca tcggcatcct gcttggatac cttacgaaga
     2941 tcaaccagcc ccagcttaaa cttcacgtat ggatcggcgc aggagcggca ttactggtta
     3001 gtatcctgtt ggccgcggtc ttccagaccc tggctattca attcgatggg gaaaccgctc
     3061 aactctttga agctggaata gcattgttgg cggttgccgt attgattacg tgttgttctt
     3121 cggtcgaaaa atttttcttg gcgcgcgtag cagcatcccc ggcaggtgag gttatgtcta
     3181 tttattcttg ctcctgtctc tggcggggac tgttatgccg tcgcagctga tgtaactatc
     3241 taaagcttca ctgcttctgg cggcagacag ccaccccgca atctcacgga atccgatttt
     3301 gcgcgtgatt gccgcagtga tgacccatgc ttcaacttca aaatccccat tagcgatcga
     3361 tccgggcctg gtgcgcctct ctgtcggcat ggagaatgcg aatgatttgt tagcagatct
     3421 agcagaagca ttggagtaga ggttatataa gcaaaatagc ctatcctcga aaccgcagga
     3481 tggcgctaat ggctaccaat tatgtcgaat ataattcaat attatttaaa ggaggaaccg
     3541 gtttcgtgag ggtgtttcta ttcttgttgt taatatcgat tacattgatc ctatccgcct
     3601 gttccgccca gcaatcttct cccacgcttg catcggaggt tgagctgggg atgggcgcaa
     3661 ccactggagc agccacaccc acgccgggca gtatgattgc cacgaaccct gaagctgtcc
     3721 ccatgatgga gccaatcaga ggcgcaaatg tccaaccggc aacagagtca aagggtgggc
     3781 agccattggc atttcatgaa gaggacgggg tcaaagtatt cgagctggac aaaagtgata
     3841 cacaagcaac caccagtaca aaagccaggg atccaataca ggcacgcgct tttattaagg
     3901 caaaccgaaa ccgggccccc gaatcgaatg caccatgaat gaaatcggtc cagctctaac
     3961 tactataaca gagaccacct caggactctc ttgcctggaa cgctggagat accgcttcag
     4021 ccgccattgg atcctcatct tcagcgtgat cttagggctt tacgcgggtt tgccgtttct
     4081 ggcacccgtt tttatgaggt tgggctggga agcccctggt agagtgattt atctcatcta
     4141 ttcattcctt tgtcaccagc ttccccagcg ctcgttcttc ctgtttgggc caaatgcgac
     4201 atattccttg actgagattc aaacgatttg gcaaaatacg gcagacccgc ttatcttgcg
     4261 ccagtttatg ggaaacgctg agatgggctg gaaagttgcc tggtccgacc ggatggtcgc
     4321 aatgtatatc agcttgtggt tgtttggtat attatggcgt ccactgcgta gttggctgaa
     4381 gccattaccc tggtgggggt ttggtttatt cctgctaccc atggcgattg acggtacgag
     4441 ccatttcgtc agtgatttgt atggtattgg tcaggggttc cgcgacagta atgcctggtt
     4501 agcttcgctg accattaatg cctttcctcg tgacttttat gccggcgatg ccttaggatc
     4561 ctttaattct tggatgcgtt tgctaacggg cgttctgttt ggcatgggga tcgtccggtt
     4621 tatttttccc tatattgata ataccttttc tcaacaggcg cggaaattgg aaagcaggct
     4681 atactacttc caacagaaga aattggacgt ctcgataagc ccttcttcgg ggcagcaatt
     4741 accatgaccg aaacatgttc gtgcccacct gaagccggaa acgatagctg cgaattacac
     4801 aacaacgttt ccgactcatc ctgccccgtc tgcctgaaaa ccggcaaacg agttgactca
     4861 cttactctga aggctttgtt aaacgtccca cttactgaag tacgccctgt acagtatcgt
     4921 ttttgtagta cggcggactg tccggttgta tattacagtc aagatggtgc acagaccttt
     4981 ctggaacccg acttgcgtga gcgtgtgtac cagaaacacg ctgctgagga tgacgtattt
     5041 gtatgctact gcttccggca tacaacaagg agcatcaatg cagaattcgc cgccaatcga
     5101 gccgaaacga ttgtagccag gatcaacgcc ggcatccagg ccggtcaatg tgcttgcggc
     5161 attcgcaatc cacagggaag ctgctgcctt ggaaacgtca aggcgttcat tagaaatttg
     5221 gaggctattc aatcatgatt ccaacagcca caccgatccg cgtcctgctt gccgacgatc
     5281 acaatatcgt ccgggagggt attcgccagg ttctggaaca tgccagtgac atccaggttg
     5341 tcgctgaagc cagtgatggt gaggttgccc aggatctgct ccggcagcac caccccgatg
     5401 ttgctgtctt agatatccaa atgccaaaag ccagcggtat cgaagtaacc cgctgggtgc
     5461 gggcaaacat gaaggatgtt ggggtcctcg tgcttactgc ctatgatgac gacccctatg
     5521 tgatggctgt gcttcaagcg ggggcaaacg gttatgtact taaaaccgcc tcgccggcag
     5581 agcttatcca ggcagtacgg gatgtccacg aaggcaaatc ggtaatagac gcgtccatcg
     5641 ctcaaaagtt gatgtcgcac ttcttcaccg gttcaaaaac aacagtagtc gagcagctta
     5701 ccgaccgcga gttggaagta ctctcgctca ctgccaaagg ttacaccaac aaagccatcg
     5761 ggatgcagtt gggcatcagc gaccggactg tgcaaggtca cctggcgcat atcttcgaca
     5821 agctccaggc cagcagccga actgaagctg tcatgcgggc tgtttcctta ggctggatat
     5881 ctcaaaacgt aggcacgatc gccgaagaat aatcatctta tgtcccaatc cccaagagga
     5941 ataaagcgag tttttttgcc ctggcgtggg ctgatctggc agttgtttgt aataaccatt
     6001 ttacctttgg cggtgctcac ggtcgtcatt accttcggta gtcttacgat tcaccagcag
     6061 gccatgcgcg ccctggttgg aaatcgtgac gaacgcgcag tcagaacggc agcggctgcc
     6121 ttggaagaac aattatcaca tcgccaggat gctattcgta gcctggcctt gctcgcagag
     6181 aaagccaccc ctgaagaatt ctcgaaggcg ttttccaact tcgcctacct tcgagatgat
     6241 tttgattcgg gtctggcatt ctttagcccc gagggtttgt tggagagcta cactggtgac
     6301 cagcaagttt gggagggtct tgtggatcag gtcgggccaa tgatccgcga gcttctagaa
     6361 cagagtaata acccagttct catttccagt gcttataaca acccggccaa ccaggaaaaa
     6421 atagtgctcg tgctggcagc ttcgtcagag cggaatttgg ttgcagcagg ggcttcttca
     6481 gcagccgatc tgatgcgcca cacgctgacc aactctttcg ccagcggatc ggaagcatcg
     6541 gttgtcgttg tggatgcaaa taaggatgtg ctttatcaag gaggctcatt ctcatatatt
     6601 ggaggtgcgg ctgcgcatcc tggcgtcgct gaggctctgc gcggcgagag cggcgttacc
     6661 tacgttgaag tgggagacag tgaacacgtg gtgacttaca gccctattgg gtctgtggga
     6721 tgggccctgg ctctggaaga accctgggaa acggttgata ctcctacctt gcgtacaact
     6781 cagctggcgc cattggtgct ggttcctgta ctcctgctga cgcttctggc gttatggttt
     6841 ggcttgcgct cgatcgtcaa gccactgcaa gccctcgagt cgaaagcctc aacattggcc
     6901 tggggagagt tcgatgccat tgaacagtct gttggaggaa tcgctgaaat ccgccggtta
     6961 caatatgagc tgatccacat ggcgcgcaag gtcaaggccg cccaacaaag cttgcacagc
     7021 tatattggtg cgatcacttc cgggcaggaa gaagagcgtc gtcgccttgc ccgcgagctg
     7081 cacgatgaca ccatccagtc cctgattgcc ctcaagcgta gggtcgagct cgcccaatta
     7141 gcctcgaaga acggtacttc tgaagcatct ttggtagagg tcgtatcctt gaccgaaaag
     7201 accattgaaa acgttcgccg ccagacgcgc gccttgcgcc caatctacct ggaagacctg
     7261 ggactggtca cggcactgga aatgcttgcc cgcgaaacca gccaggctgc aaatattccc
     7321 atcgatttcc aacgccaggg tacggagaat cggttgaatc ccaatgccga gctggctctt
     7381 taccggatgg cccaggaagc attgaacaat gttatccggc atgctcaggc ctcccaggcc
     7441 aatctgggca tatcttttac atcccaggaa gtcacattgt acgtaagtga caacggcaga
     7501 ggcttcgatg tacccaaaag tccggccgag ttcgctcctg gtgggcactt tgggctgtta
     7561 ggcatgtacg aacgtgccga attgattggc gctcggttag agatccattc cactcctgaa
     7621 caaggaagcc agattacagt tcgtttaccg gtttgattca accgtattaa agcaattgct
     7681 tttggaagaa caacttactg ggtataagca taatggctcg tttccgaatc ggcctgatca
     7741 cattaagatt actggagtta gatagtctgg atggagcaat ctaatgaccc gaataacggt
     7801 gatattcttg tttctcgttg cctcgttgct atccgcgtgt aagagcactg caatatctac
     7861 gccggccgta gacgtagttg ggcagcaggt atcgatcgct ggcggatcgt atacggacgt
     7921 ttctgtagac gagttgcaat ccatgctggc gaacaaagat ttcaccttcg tcaacgttca
     7981 tattcccttc gaaggggata ttgctggtac cgatctctcg atcgcctacg atgagatcga
     8041 acaaaacctg gatcaactgc ctgcagagaa agacgctaaa attgtgctct attgtcggag
     8101 tgatcggatg agcaggatgg cagccgagac gctcgttggc ctgggctaca ccaacctttg
     8161 gaacctggat ggcgggatgg ttgcgtggga aaaggccgga cagccattgg aacgatagac
     8221 cgtggatcat ccaatcacac ccctcaatcc acccaccctc agtcgccggc tcacgacttt
     8281 tcttcacgcc gtgaccttcg tattgggctt ttcactggtc tttatcgtcg gctggggcgg
     8341 agctgcgaca actgcagggc
//
LOCUS       DATMXK010000048         2233 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_123279, whole genome shotgun sequence.
ACCESSION   DATMXK010000048 DATMXK010000000
VERSION     DATMXK010000048.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2233)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2233)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2233
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123279"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1320
                     /locus_tag="VN363_00945"
     CDS             <1..1320
                     /locus_tag="VN363_00945"
                     /EC_number="1.11.1.5"
                     /inference="COORDINATES: protein motif:HMM:NF015131.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c peroxidase"
                     /protein_id="HWQ83097.1"
                     /translation="ALAQEPVPEPEGLAALGEALFYDKSLSANGTQSCAACHSPEVGF
                     TGPDAAINTGGAVYQGALPNHFGNRKPPSSAYAGDSPLFHFDETEGGWFGGMFWDGRA
                     TGAVLGDPLAEQAQGPYLNPLEQAIPNAQVLCVKVRQAGYAGLFEQVWGAGSLDCARD
                     TAGVYEMIGRSVAAYERSPEVNPFTSKFDLFWDNAAAAGKDVTKIKCGTGGMGGGGGG
                     GMGDPMGGAMGCPGGGMDPNRWTAFRSLGLTDADLVGLAVFNARAECSACHTLEPGPA
                     GYPLFTDFGYDNIGVPKNPDNPFYSMPAAWNPDGAAWIDGGLGDFLKSAGYPAEVYAA
                     ELGKIKVPTLRNVDLRPSPDFVKAYGHNGYFKSLEDIVFFYHWRGMMDSMCADGGGME
                     GMCEMMSQMFPPPEVDENRAVLGMFPRPQVDNIVAFLKTLSDGYFVR"
     gene            1395..>2233
                     /locus_tag="VN363_00950"
     CDS             1395..>2233
                     /locus_tag="VN363_00950"
                     /inference="COORDINATES: protein motif:HMM:NF019352.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWQ83098.1"
                     /translation="MNKKYFSRRDFLKFTGIGAAALVSAGFRPLGWDITAGAPTPGVV
                     ARGIASDPAQMMEFIPDAEIAITAAEKWVQILPGAQTRVWSYEGQLLSGSGATVQNLP
                     GNYLGPILRVESGKKVRIFFHNNLNEDSVIHPHGLRVPEDCDGHPMQAIPPGATKIYE
                     FEVIDRAGLYWFHPHPHMRTAEQVAMGMAGLFHVWDAEEALAVPGASTGANDIPVIIQ
                     DRVFDANNQIVYQDHPMWGYLGDRILVNGALNPTLALEPRGYRLRLLNGSNARTYKLA
                     WSNG"
BASE COUNT          435 a          674 c          727 g          397 t
ORIGIN      
        1 gcgctggccc aggagccggt tcctgagccg gaagggctgg ctgcattggg ggaggcgctc
       61 ttctacgata aaagcctctc ggcgaacggc actcagagct gcgcggcctg tcactccccg
      121 gaagtgggct ttaccgggcc ggatgcggcg atcaataccg gcggggcggt ctaccagggc
      181 gctctgccca atcacttcgg caaccgcaag ccgcccagct cggcctatgc cggtgacagc
      241 ccgctgttcc acttcgatga aaccgagggg ggctggttcg gaggcatgtt ctgggatgga
      301 agggcaacgg gcgcagtcct tggcgaccca ctggccgagc aggctcaggg gccttacctg
      361 aacccgcttg agcaggcaat cccgaacgcc caggtcctgt gtgtgaaggt caggcaggcg
      421 ggatatgccg gcttattcga gcaggtctgg ggagcaggct cgctcgactg cgccagagat
      481 accgccgggg tctacgagat gatcggccgc tcggtggcgg cttacgagcg cagcccggag
      541 gttaatccat tcacctccaa attcgacctg ttctgggaca atgccgcagc cgccggcaag
      601 gatgtgacca aaatcaaatg cggtacgggc ggaatgggcg gcggtggtgg cggcggcatg
      661 ggcgacccca tgggaggagc tatggggtgc cccggcggtg ggatggaccc caaccgctgg
      721 acggctttcc gcagcctggg tctgaccgac gccgatctgg ttggcctggc ggtcttcaat
      781 gctcgggcgg agtgctcggc ctgccacacc ctcgagccgg gtcccgctgg ctaccccctg
      841 ttcaccgact ttggctacga taatatcggc gtcccgaaga atcccgacaa cccgttctac
      901 agcatgcctg cagcctggaa tccagatggc gcggcctgga tcgacggcgg cctgggggat
      961 ttcctgaaaa gcgccggcta tcccgccgag gtctacgcag ccgagctggg taaaatcaaa
     1021 gtgcccacgc tgcgcaatgt ggatttacgc ccgtcgccag actttgtgaa ggcttacggg
     1081 cataacggtt actttaaatc cctggaagac atcgttttct tctaccactg gcgcggcatg
     1141 atggacagta tgtgtgctga tggcggcgga atggaaggga tgtgcgagat gatgagccag
     1201 atgttcccgc cgcccgaagt ggacgagaac cgcgccgtat tgggcatgtt cccccgcccg
     1261 caggtggata acattgtggc ttttctaaaa acactttccg atggttattt tgtccgctag
     1321 atagactgcc gcaggaggag cgagcactgc tccgcctgcg gctaaaaggc atggagggaa
     1381 aagagggagg gatcatgaac aagaaatatt tcagccggag ggatttcctg aaattcaccg
     1441 gaatcggcgc ggcagcgctg gtcagtgccg gttttcgtcc gctgggatgg gatatcaccg
     1501 caggcgcgcc caccccgggc gtcgtcgcca ggggcatcgc ctctgatcca gcgcagatga
     1561 tggaatttat ccccgatgcg gagatcgcca tcacggccgc cgaaaaatgg gtccagatcc
     1621 tgccgggggc gcagacccgc gtatggagct acgagggaca gctcctgagc ggatcgggcg
     1681 caaccgtgca gaatttgccg ggtaactatc tggggccgat cctgcgagtg gaaagcggta
     1741 aaaaggtgcg catcttcttc cacaataatc tcaatgaaga cagcgtcatt cacccgcacg
     1801 gcctgcgcgt gccggaggac tgcgacgggc accccatgca ggcgatcccc ccgggagcga
     1861 ccaagattta cgagttcgaa gtcatcgacc gggcagggct ttactggttc catccccacc
     1921 cgcacatgcg caccgccgag caggtggcga tgggtatggc gggtttgttc cacgtgtggg
     1981 acgccgaaga agcgctcgcc gtgccgggcg ccagcaccgg ggccaacgat atcccggtga
     2041 tcatccagga ccgcgtcttc gacgccaaca accagatcgt ctaccaggat cacccgatgt
     2101 ggggctacct cggcgaccgc atcctggtca acggcgcgct caatcccacc ctggcgctcg
     2161 agccgcgcgg ctaccgcctg cgcctgctga acggatccaa cgcccgcacg tacaagctgg
     2221 cctggagcaa cgg
//
LOCUS       DATMXK010000049         4427 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_44748, whole genome shotgun sequence.
ACCESSION   DATMXK010000049 DATMXK010000000
VERSION     DATMXK010000049.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4427)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4427)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4427
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44748"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1019)
                     /locus_tag="VN363_00955"
     CDS             complement(<1..1019)
                     /locus_tag="VN363_00955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83099.1"
                     /translation="MNTITEEKIAAIRREKPRLGLIFGLLAGAGFALTAWGLDANQLA
                     QAHAYLPWGKFIAGLVICLVVGGFTGWVASRLDSSLWAAFLWALVGCTFTIGLSWLAY
                     KGFLSLFNWFYPDLASQMDYASNPAITLLTGIAVVASGILGIGVGIAQIIQVESALAG
                     VNPFQRLVRLAMGALIFAIAGAISSSLVQTKLAEPVLQVNDALVYAREHRGQILEIKE
                     ARKYGLTALRALGDLQYQPYRLVVSSYDEYLSMIWVMLDFGDVQTRCSVLEKTLSYCE
                     SPPEIAASSSSGNSVSIPGPQPTGIPSAVPTSTPAGSSTDTASGEQVNPPENIPPPTD
                     PALDSV"
     gene            complement(1016..2419)
                     /locus_tag="VN363_00960"
     CDS             complement(1016..2419)
                     /locus_tag="VN363_00960"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWQ83100.1"
                     /translation="MRSSTLNFTTGNKPSSHLKRNNNMQRSNLTLAVLILSVLSLAAC
                     SQTAGTSIPGIPTAAATSLPQPAVAITQAPTPTEALVEPAGPTSTAPVSPEPSATQTT
                     GSAQVTSFPNPDLFTWIEVASGLTAPTSLAHAGDERLFIVERDGRIRILQAGSLKPEP
                     FLDITDRVGAGGERGLLGLAFHPRYPETGAFYVNYTDFNGNTHISQFSVTANNPDQAD
                     AASEKELIFVEQPFPNHNGGALAFGPDGFLYIGLGDGGSAGDPHGNGQSVDTLLGKIL
                     RISVDQGDPYGIPDGNPFISGGGRPEIWAYGLRNPWRFAFDSLTGDFYIGDVGQNIHE
                     EIDFQPASSQGGENYGWNILEGSACFISEPCDSAGLTAPVFTYTHAEGGCSVTGGGVY
                     RGSELPEWQGIYLFGDYCSGMVGGLLPPANQGDGAGWQRAWLFTDVGSISSFGEDASG
                     EIYLADLGGSIYRLVRK"
     gene            complement(2382..2741)
                     /locus_tag="VN363_00965"
     CDS             complement(2382..2741)
                     /locus_tag="VN363_00965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83101.1"
                     /translation="MNDPQGPKMIRPQGGAFNDLTQYAKLVVRLMGDRRVNPLLKLLP
                     IGSLVYLVLPDLLPGPIEDAGIIWIGTYLFVELCPPAVVAEHRAALQEVIPGEWRDPQ
                     KPKPEDEIIDAEFHDRE"
     gene            complement(2918..3265)
                     /gene="rplT"
                     /locus_tag="VN363_00970"
     CDS             complement(2918..3265)
                     /gene="rplT"
                     /locus_tag="VN363_00970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558491.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L20"
                     /protein_id="HWQ83102.1"
                     /translation="MARVKTGPYRHHRHLKVLKLTKGQFGSRSTLYRRANEAMLKSLW
                     YAYRDRRTRKRDLRRLWIVRINAAARINGISYSRLIHGMKTNHIRINRKMLADLAARD
                     PKTFSAVVAQAQS"
     gene            complement(3403..3630)
                     /locus_tag="VN363_00975"
     CDS             complement(3403..3630)
                     /locus_tag="VN363_00975"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bL35 family ribosomal protein"
                     /protein_id="HWQ83103.1"
                     /translation="MTRKARTGKYKIKTHKATSKRFALTGGGMLMRTKGGKSHFRRRR
                     SARVKQQLDEMISVKGRGIVKTVRRLAPYMK"
     gene            complement(3730..4257)
                     /gene="infC"
                     /locus_tag="VN363_00980"
     CDS             complement(3730..4257)
                     /gene="infC"
                     /locus_tag="VN363_00980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00168.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003743 - translation initiation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006413 - translational initiation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-3"
                     /protein_id="HWQ83104.1"
                     /translation="MSTTNYRVNDAIRVPQVRVIGAKGENVGVITIEEALRLAQEAEL
                     DLVEVAPNSDPPVCRVMDFGKFIYERAKKEKEAKRAQTKIEVKEIRLRPKTNDHHRDL
                     KVRDARRWLEDGMKVKVRIRFRGREISYPEIALSDLKSIADELADIALVEQSPGLEGR
                     TMLMVLAPGKAPKKK"
BASE COUNT          960 a         1230 c         1229 g         1008 t
ORIGIN      
        1 acagaatcca gagcgggatc ggtcggggga ggtatattct cgggtggatt aacttgctcg
       61 ccagatgctg tatccgtgga gctgccagca ggggtgcttg ttggcactgc ggatgggata
      121 ccggttggct gagggccagg tatggaaacc gaatttccgc tgctgctgga ggcagcaatc
      181 tcgggcggtg attcgcagta ggagagggtc ttttccagca ccgaacagcg ggtttgcaca
      241 tcgccaaagt cgagcattac ccagatcata gacaggtatt catcgtaact gcttactacc
      301 aggcggtagg gctggtactg cagatcgccc agggcgcgca gggctgtcag cccatatttg
      361 cgagcctcct taatttccag gatctggccg cggtgctccc gggcatatac gagggcgtca
      421 ttaacctgga ggacgggctc ggccagtttt gtctggacca ggctgctgct gatcgcacct
      481 gcgatggcga agatcaatgc ccccatcgcc agccttacca ggcgctggaa tggattcact
      541 cctgccaatg cggattcgac ctggatgatt tgagcaattc ccaccccaat cccgagtata
      601 ccgctggcta ctaccgcgat cccggtcaga agtgtgatcg ctggattgga cgcgtaatcc
      661 atctggctgg ccaggtcagg gtagaaccag ttgaacaggg agagaaagcc tttataagcc
      721 agccaggaaa gccctatggt gaatgtacag ccaaccaacg cccataaaaa ggcagcccac
      781 aggctgctgt ccagccggga tgccacccag ccggtaaacc cacccacaac caggcagata
      841 accaatccgg cgatgaattt accccagggc aggtaggcat gggcttgcgc aagctggtta
      901 gcatccagtc cccaggcggt cagggcaaaa cctgcgcctg ccagtaagcc aaagattaag
      961 cccaggcgag gtttttcacg ccggatcgcg gcgatttttt cttcagtgat ggtgttcatt
     1021 ttctgacgag cctgtatatg gatccgccta aatcggccag gtaaatttca ccgctggcgt
     1081 cttcaccgaa cgagctgata ctgcccacgt ctgtgaacag ccaggctcgc tgccagcctg
     1141 cgccatcacc ctgattggca gggggcagca agccgcctac cattccagag caatagtccc
     1201 cgaacaagta gattccctgc cattcaggca actccgagcc gcggtaaact ccgccgccgg
     1261 taacggaaca gccgccttcg gcatgcgtat aggtgaacac cggggcagtc agaccggcag
     1321 aatcgcaagg ctcgctgata aaacatgcac tgccttccag gatattccag ccataatttt
     1381 cacccccctg gcttgaggca ggctgaaaat cgatctcttc gtggatgttt tggccaacat
     1441 caccgatata gaaatcaccg gtcaggctgt caaacgcgaa acgccatggg ttgcgtaatc
     1501 cgtatgccca gatttccggg cgtccgccgc cgcttatgaa tggatttcca tccggtatcc
     1561 cgtaagggtc accctggtcc acagagatgc gcaggatctt tcccaacagc gtatccacgg
     1621 actgcccatt cccgtgtggg tcgcctgcgc tgccaccatc tcccagtcca atatacagaa
     1681 agccatccgg tccaaaagcc agcgccccgc cgttgtgatt gggaaatggc tgttcaacaa
     1741 agatcagctc tttttcgctg gcagcatctg cctgatcagg attgtttgca gtaacgctga
     1801 actgcgaaat atgggtattc ccattgaagt cggtatagtt cacatagaac gcacccgttt
     1861 ctggatagcg ggggtgaaat gccagaccca gcagaccgcg ctcaccaccc gcaccaaccc
     1921 ggtcggtgat atccaggaaa ggttctggct tgaggctgcc tgcctgcagg atacggatcc
     1981 gcccgtcgcg ttctacgata aaaaggcgct catccccggc atgggccagg ctcgtgggag
     2041 ccgtcaggcc gctggcgact tctatccagg taaacaaatc gggattgggg aagctggtta
     2101 cctgagctga gccagtagtt tgcgttgcgc tgggctctgg cgatacaggt gctgtggatg
     2161 taggaccagc tggttctacc aacgcttcgg ttggtgttgg cgcctgggtg atagctacgg
     2221 caggctgggg gaggctggtg gctgctgcag tagggatgcc tggaatcgat gttccggctg
     2281 tctggctgca tgctgccagg cttaagacgc tcagaatcaa cactgcaaga gtcaggttac
     2341 ttcgctgcat attattatta cgcttgagat gggaggatgg tttattcccg gtcgtgaaat
     2401 tcagcgtcga tgatctcatc ttccggcttt ggcttttggg gatcgcgcca ctcgccggga
     2461 atcacttctt gcaaagccgc ccggtgttcg gcgacgaccg cgggtgggca cagctccaca
     2521 aataaatacg tgccaatcca gatgatcccg gcatcttcga ttggccccgg gagcaggtct
     2581 ggcagcacca gatagaccag ggagccaatt gggagcagct tgagcaaagg atttacccgc
     2641 cggtcgccca tcaggcgcac aaccagcttg gcgtactggg tgagatcatt aaatgcaccc
     2701 ccctgggggc gaatcatttt tgggccttga ggatcgttca tggttgcctc ctgatggagg
     2761 tgcatctccg aatggaagta cgcctccacg cgaaaagatt atactacacc gggcaggggg
     2821 ccaactcggg cgtgcctccc gaggcatgtc tgcggctttg gaaataaaac ggggcggctg
     2881 ctgccgcccc atggggtgtc cgtgagttgt gattgggtca ggattgtgcc tgggcaacca
     2941 cggcgctgaa agtttttgga tcccgcgcag ccaggtctgc cagcattttg cggttgatcc
     3001 gaatgtgatt tgttttcata ccatggatca aacggctgta cgaaatgcca ttgatgcggg
     3061 cagcggcgtt gatgcgcaca atccacaggc ggcgcaaatc gcgtttgcgg gtgcggcgat
     3121 cccggtaggc ataccacaaa cttttcagca tggcctcgtt cgcccgtcgg tacagggtgc
     3181 tccgagaacc aaactgacct ttggtgagct taagaacttt caggtgccgg tgatggcggt
     3241 atggaccagt ctttacacga gccattttct actctcctgc gaacccctgg gcgccagcag
     3301 ttcattctgc tcgttagctg cacccaacag tcgcactaat agacggataa aatcgatcag
     3361 cggaagccgg aaatggcgtc cgtccgggct gccagtggca gcctatttca tgtacggcgc
     3421 caggcggcgt actgttttga cgatcccgcg acctttcacg gaaatcatct catcgagctg
     3481 ctgtttgaca cgcgcggatc tgcgccggcg gaaatggctt ttgccaccct tggtgcgcat
     3541 cagcattcca ccgcctgtca gggcaaaacg cttcgacgtc gccttatgcg tcttgatctt
     3601 gtatttgcct gttcttgctt tgcgagtcac tttccgaact cctctcacat aaaattccgc
     3661 ggacaggttc atccgcggac atcaacgggt caattattat accggataat gatgatttca
     3721 gtgggccaat tactttttct tgggagcttt gccaggtgcc agcaccatca acatggtgcg
     3781 cccttccagc cccggggact gttcaactaa agcaatatct gccagctcgt cggcaatcga
     3841 tttgaggtca ctcagggcga tctctggata gctgatctca cgcccgcgga agcggatccg
     3901 aactttgacc ttcatgccat cttccaacca gcggcgagca tcgcgcactt taagatcgcg
     3961 gtggtgatca ttggtcttgg ggcgcaggcg gatttctttg acttcgatct tggtctgggc
     4021 tcttttcgct tccttttctt ttttggcgcg ctcataaatg aacttgccaa aatccatcac
     4081 ccggcaaacc ggtgggtcgg aattgggcgc tacttcgacc agatcaagtt ccgcttcctg
     4141 ggcaagacgc agggcttctt cgattgtaat taccccgaca ttctctcctt tagcgccaat
     4201 cacacgcacc tggggcactc gaatggcatc attgacacgg taatttgttg tgctgataag
     4261 ctttctcctt aatcaagttg gttaataaat aaaattaaaa ttacggggaa tcaaccgcaa
     4321 gcatcatacc aaacatttaa cgaatcgtca acgctcgcgt atcgccacga aaacagccgc
     4381 tgaactgctc aacggctggt gtgtgggcgg tataggactc gaaccta
//
LOCUS       DATMXK010000050         4271 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_87710, whole genome shotgun sequence.
ACCESSION   DATMXK010000050 DATMXK010000000
VERSION     DATMXK010000050.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4271)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4271)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4271
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87710"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..225
                     /locus_tag="VN363_00985"
     CDS             <1..225
                     /locus_tag="VN363_00985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83105.1"
                     /translation="VERNSVELQCEISLLFLPRDIAEQDAPLANLGYKARSIQSLGVR
                     AWQEAASESMPPDSVMLFKQLRKDRVLRPV"
     gene            244..1125
                     /gene="cdaA"
                     /locus_tag="VN363_00990"
     CDS             244..1125
                     /gene="cdaA"
                     /locus_tag="VN363_00990"
                     /EC_number="2.7.7.85"
                     /inference="COORDINATES: protein motif:HMM:TIGR00159.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004016 - adenylate cyclase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diadenylate cyclase CdaA"
                     /protein_id="HWQ83106.1"
                     /translation="MPAFLSDLLANILFIFERVDLLDVIDLLLVTTIFYAILRLLRDT
                     QAMVLLRGVLLLIALFGLISSLDVLPAFSWLVETTLPALVVAIPVIFAPEIRRALERL
                     GRAGVIFTPEKPNAPALDTISAIVTAASRLSDRQHGALVVIQRLDTLDDYIRTGVKMD
                     SLVTPELLLQVFYPNTPLHDGAAIISGERMVAAACVLPLSASGVLTRSPERQMGLRHR
                     AALGISEVSDAVAVVVSEETGSISIVHGGRMIRRLDPERLENILRAFFKPAKTQQTLK
                     EFVGRLMNPQPPDAEAK"
     gene            1125..2390
                     /locus_tag="VN363_00995"
     CDS             1125..2390
                     /locus_tag="VN363_00995"
                     /inference="COORDINATES: protein motif:HMM:NF019561.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CdaR family protein"
                     /protein_id="HWQ83107.1"
                     /translation="MFKIALQWFSRNISTLLLAFAIAVVVWVSAVLTADPNEERIYTR
                     NLSVVGQSSNLMQMTELPTQVRITLKAPRSILTELNNNPALIQATLDLSGLDGGNHQA
                     PIEVQVDDSPVRVLLVEPEQVEVLLEKQVSRTTPVQLEIIGEPPIGYRKGTVQASPDE
                     VTITGSESQVNQVDSVVARLDITGLSDTIQTNLPVTALNSAGDIVSEVAISPRAIAVT
                     QPINLLGGFRSVVVTVITEGQIEAGYRLTNISVSPRTVTVFSSDPQVVADLPGFVETM
                     PLNLNSINDDLEVRVQLDLPDGVELVNEESVLVQIGVAAIEGSVTVTVPIEAFGQSPN
                     FAAQIAPKLVDLIVSGPIPVLDLLTSDSFRVLVDINGLTLGTYQLPIIVDLVPDEVVV
                     DAILPETVEVTITPPATPTVTPIPVTPTP"
     gene            2416..3789
                     /gene="der"
                     /locus_tag="VN363_01000"
     CDS             2416..3789
                     /gene="der"
                     /locus_tag="VN363_01000"
                     /EC_number="3.6.5.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290913.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome biogenesis GTPase Der"
                     /protein_id="HWQ83108.1"
                     /translation="MRKPIVALVGRPNVGKSTLFNRLAEERLAVVDDTPGTTRDRLVA
                     EAEWTGIVFNIVDTGGIDPEDYSGKNRQPLSVGSADFIQQIRQQAEIAIQEADAVLFL
                     TDADSGMTPADREVAQILRRYQTEKDGQLWPPIFLVVNKADSEARRLQALQFYELGVD
                     EIYPISALHGTGTGDLLDALVASFPSQEEEIEDETVKISIVGKPNVGKSSLLNYLLGE
                     ERVIVSPIAGTTRDAIDTSLEYEGIKITLIDTAGIRRRGKVEPGVEKYSVLRAMQAIE
                     RSDVSLLMIDATSGITAQDTHIAGYILESWKSTVVVVNKWDAIVKDTHTMNEHTAQVR
                     QQLNFMDYVPILFISALTGQRVEQVLPMALRVQEQRLLRITTSSLNQIIQRAQDTHPA
                     PSHAGRQLKIYYGTQVRSDPPTFLLYVNDPHLSHFSYMRYLENRIRETHPYLGTPIRI
                     VMRPRRE"
     gene            3862..>4271
                     /gene="lepB"
                     /locus_tag="VN363_01005"
     CDS             3862..>4271
                     /gene="lepB"
                     /locus_tag="VN363_01005"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: protein motif:HMM:NF021981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWQ83109.1"
                     /translation="MEGYRTETAQEPLAETPSKRLGFVKLLVDLVETILLAVLLFIGI
                     NAVSARIRVDGFSMLPTLQSHQYVLVNRLAYKVGSPQAGDVIVFHYPRDPQQEYIKRV
                     IGLPGDRVEIANGEVLINNQLIDEPYIAAAPVYQT"
BASE COUNT          965 a         1164 c         1143 g          999 t
ORIGIN      
        1 gtggagcgga attcagtaga gctgcagtgc gaaatctcgc tgctcttcct gccccgggat
       61 atagcggaac aagacgcccc gctggcgaac ctgggttata aagctcgcag tatccagagc
      121 ctgggggtgc gcgcctggca ggaagccgcc agcgaatcga tgccaccgga tagcgttatg
      181 ttatttaagc agcttcgcaa ggatcgggtg ctgcgcccgg tttagaattg attggagtaa
      241 tgggtgccgg cttttttaag tgatctgctc gccaacatcc tgttcatttt tgaacgtgtg
      301 gacttgctgg atgtcattga cctcttattg gtcacgacaa tcttttacgc aatcttacgc
      361 ttactgcgcg atacgcaagc catggtgctc ttgcgcggtg tgctgctgct gattgcactt
      421 tttggtttga tcagctcgtt ggatgtcctg ccggcctttt catggttagt tgaaaccact
      481 ttgccggctc tggtggtcgc aatcccggtc atctttgccc ccgagattcg ccgggctctg
      541 gaacgcctgg gccgggcagg tgtcattttt actccggaaa aacccaacgc gccggctttg
      601 gataccatca gcgccatcgt taccgccgct tcccgccttt cagaccggca gcacggcgcc
      661 ctggtcgtga tccaacgcct ggacacgctg gatgactata tccggaccgg ggtgaagatg
      721 gactcactgg ttacacctga attactcttg caggtgtttt accccaatac acctctgcac
      781 gatggcgcgg caatcatttc gggggagcgc atggttgctg cagcgtgcgt actgccgcta
      841 tctgcctccg gtgtgctcac gcgttcgcct gagcgccaga tgggcttgcg ccaccgggca
      901 gccctgggaa tttctgaagt ctcggatgcc gtggcggtcg ttgtctctga agaaactggt
      961 tcgatttcaa ttgtacatgg tggtagaatg atccgccgcc tggatcctga gcgattagaa
     1021 aatatcctcc gtgcattctt caagcctgcc aagactcaac aaaccttgaa agaattcgtg
     1081 gggcgcttaa tgaacccgca acctccagat gcggaggcaa aataatgttc aaaatcgccc
     1141 tgcagtggtt ttcgcgcaac atatccaccc tgctgctcgc atttgcaatt gcggttgtgg
     1201 tatgggtttc ggctgtgctt accgcggatc caaatgaaga acgcatttat acacgtaacc
     1261 tcagtgtagt aggccaatcg tcaaatctga tgcagatgac ggagcttcct acccaggtgc
     1321 gcatcacact gaaagcacct cgctcgatcc tgaccgagct aaacaataat ccagctctca
     1381 tccaggccac gcttgacctt tctggcctgg atggtggcaa tcaccaagcc cccattgagg
     1441 ttcaggtgga tgacagtccg gtacgggttt tactggtgga gccagaacag gtcgaagtac
     1501 tgctggaaaa acaagtctcc agaacgacac ctgtccagct cgaaatcatc ggtgagcccc
     1561 cgattggcta ccggaagggt accgtgcagg cttccccgga tgaggtaacc attactggat
     1621 ctgaatccca ggtcaaccag gtcgatagtg tggtcgccag gctggatatc accggtcttt
     1681 cggatacgat ccagacgaac ttaccggtca cggctctcaa cagcgccgga gacattgtga
     1741 gtgaggtagc gatatccccg cgtgcaatcg ctgtcaccca gccgatcaac ctgttgggtg
     1801 gtttccgctc agttgtcgta accgttataa ccgaggggca aattgaggct ggttatcggc
     1861 tgaccaatat ctctgtcagc ccgaggacag taactgtatt ctccagcgat ccccaggtcg
     1921 ttgccgactt gcccggcttt gtggaaacca tgccattaaa tctgaattca atcaacgatg
     1981 accttgaggt ccgcgtgcag ctcgatctgc ccgatggagt cgagctggtt aatgaagaga
     2041 gtgtattagt acagatcggg gtagcagcta tcgaggggag tgtgacggtc acagtgccga
     2101 tcgaggcttt tgggcaatcc cccaattttg ccgcccagat cgcgccgaaa ctggtagatc
     2161 tcattgtctc cggtccaatt ccggttttag atctgctgac atcggacagc ttccgggtgc
     2221 ttgtagatat caacggctta acccttggta cttaccagct gcccataatc gttgatctgg
     2281 ttcccgacga agttgtcgtg gatgccatct tacccgagac ggtggaggtg acgatcacgc
     2341 ctccggcaac cccaacagtg acccccattc cggtaacacc taccccataa agcttatcca
     2401 gataaggtat tttgaatgcg taaaccaatt gtggccctgg tgggccggcc gaatgtcggc
     2461 aaaagcacat tatttaatcg tctggcggaa gaacgtctgg ctgttgtcga tgatacccca
     2521 ggcacaaccc gcgaccgcct ggtcgctgaa gcagagtgga ctggaatcgt ttttaatatt
     2581 gtggacaccg gtgggatcga ccctgaggat tattcaggaa agaaccgcca gcccttatcc
     2641 gttggctcgg ctgattttat ccagcagatc cgccaacagg cagagattgc catccaggaa
     2701 gccgatgcag tcctttttct caccgatgca gacagcggca tgactccagc cgatcgcgag
     2761 gttgcgcaga tattgcgccg ctaccagact gaaaaggatg gacaactctg gccgcccatt
     2821 ttcctggtgg tgaacaaagc cgatagtgaa gccaggcggc tgcaagcttt gcagttttac
     2881 gagctgggag tggatgaaat ctaccctata tccgccttac acggcacagg tacaggcgac
     2941 ttgctggacg cactcgtcgc cagttttcca tctcaggaag aagaaatcga agacgagact
     3001 gtcaagatct cgatcgtggg gaaacccaat gtcggcaagt ccagcctgct caactacctg
     3061 ctgggcgaag aacgagtcat tgtcagtcct atcgccggaa caacccgtga tgcgatcgat
     3121 acctcccttg aatacgaagg gatcaagata actctgatcg atacggctgg tattcgccgg
     3181 cgcggcaagg ttgagccagg cgtagagaaa tacagcgtgc tgcgcgccat gcaggcgatc
     3241 gagcgttcgg atgtttctct attgatgatt gacgccacca gcggaattac tgcccaggat
     3301 acacatattg ccgggtatat tcttgaatcg tggaaaagca cagtggttgt ggtcaataag
     3361 tgggatgcga tcgtcaaaga cactcacacc atgaatgaac acaccgctca ggtgcgccag
     3421 cagttaaatt tcatggatta tgtaccgatc ttatttatct ccgcgcttac cgggcagcga
     3481 gttgaacagg tcttaccgat ggcgctgcgt gtgcaggagc agcgtctgct gcgcatcacc
     3541 acttcctcgc tgaaccagat catccagcgc gcccaggata ctcacccggc tccctcccat
     3601 gccggtcgcc agctcaagat atattacggc acccaggtgc gcagcgatcc accaacattt
     3661 ctgctgtacg tcaacgatcc ccatctgagt catttcagct atatgcgtta cttggaaaat
     3721 cggatccgtg aaacgcatcc ttacctggga acaccgatcc ggatcgtgat gcgcccgaga
     3781 cgagaataag catactctca tgtagcaact cttgtccgcg agttcttcca gttttataat
     3841 cctcccgtca ggaggcagac tttggaaggt tatcgcaccg aaactgccca ggaaccgctg
     3901 gcagaaacgc ccagcaaacg cctgggattt gtaaaattat tggttgatct ggtcgagaca
     3961 atcttactgg cggtattgct ctttatcggg attaatgcag tttcggcgcg catccgggtg
     4021 gatggattca gcatgctgcc aaccctgcag agccaccaat atgtgctggt taaccggctg
     4081 gcctacaaag tcggcagccc gcaggccggg gatgtgattg tgttccacta cccccgcgac
     4141 ccgcagcaag aatacatcaa gcgcgtcatc ggactgcccg gcgaccgggt agagatcgcg
     4201 aatggcgaag ttttgatcaa caaccagttg atcgatgagc catatatcgc ggccgccccg
     4261 gtatatcaga c
//
LOCUS       DATMXK010000051         3752 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_20044, whole genome shotgun sequence.
ACCESSION   DATMXK010000051 DATMXK010000000
VERSION     DATMXK010000051.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3752)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3752)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3752
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20044"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /locus_tag="VN363_01010"
     CDS             complement(<1..621)
                     /locus_tag="VN363_01010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWQ83110.1"
                     /translation="MALFSKDLGIDLGTMYTRVVSGNQLLIEEPTVVAIVVDEQKMVA
                     WGQEAFDMFGRVPDSMEVTRPMLNGVIADYEVTEHMLRFLLRKISNRMFPPRILITVP
                     YGVTSVESRAVQEAALNAGSRDVNLIPQPLAAALGIDLPISTPSGNMILCLGGGATQA
                     AVLAMYGIVTAETMRSGGMAMDESIVNYVRKKYGVIIGQLTAEQVKI"
     gene            complement(634..1077)
                     /gene="atpC"
                     /locus_tag="VN363_01015"
     CDS             complement(634..1077)
                     /gene="atpC"
                     /locus_tag="VN363_01015"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559792.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit epsilon"
                     /protein_id="HWQ83111.1"
                     /translation="MPIRCEIVSQDRMVFEGDADIVVLPGVEGEMGILPNHAPLLTTL
                     TLGILKVRYKGEEQYFTIAGGIAEIQPDIITVLANAAENVNEIDLARAEQSRRRAEEL
                     LAKGPPPDTDAYLAMEAALRRSNLRLEAAQRYRQAHGRSGASKEN"
     gene            complement(1112..2512)
                     /gene="atpD"
                     /locus_tag="VN363_01020"
     CDS             complement(1112..2512)
                     /gene="atpD"
                     /locus_tag="VN363_01020"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_020045332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit beta"
                     /protein_id="HWQ83112.1"
                     /translation="MANATGHVVQILGGVVDVEFPAEAMPEIYEAISVPMEGGKTLIL
                     EVEKHMGDNVVRTVAMDATDGLQRGRPAYATGAAIKVPVGPATLGRVFNVLGQPVDGL
                     GPVETDEYLPIHRLAPPFSEQSTRVEVFETGVKVIDLIAPFTKGGKTGIFGGAGVGKT
                     VIIMELIRSIASVHQGNSVFAGVGERTREGTQLYREMLESGVMKDTVMVFGQMNEPPG
                     VRLRVALSALSMAEYFRDSGKDVLLFIDNIFRFSMSGSEVSALLGRMPSAVGYQPTLA
                     TEMGELQERITSTRGGSITSMQAVYVPADDYSDPAPVATFTHLDATIALERSIAEKGI
                     YPAVDPLSSTSRILDPNIVGIDHYNTAREVQQMLQRYKDLQDIIAILGVDELSEDDKL
                     IVSRARKMERFFSQPMFVAQQFTGREGRYVPLKDTVRGFREILDGKHDDVPEQAFVMA
                     GDIDEVVERSRQMAAA"
     gene            complement(2563..3456)
                     /gene="atpG"
                     /locus_tag="VN363_01025"
     CDS             complement(2563..3456)
                     /gene="atpG"
                     /locus_tag="VN363_01025"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01146.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000275 - mitochondrial
                     proton-transporting ATP synthase complex, catalytic sector
                     F(1) [Evidence IEA];
                     GO_component: GO:0045261 - proton-transporting ATP
                     synthase complex, catalytic core F(1) [Evidence IEA];
                     GO_component: GO:0045262 - plasma membrane
                     proton-transporting ATP synthase complex, catalytic core
                     F(1) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F1 subunit gamma"
                     /protein_id="HWQ83113.1"
                     /translation="MANAREVKLRIRSVKNISHVTRAMQAVSASKVRKSTEALLRTRP
                     YATKAWQVLTHIARQPGREQMHPLLVQRPSVNRVLVVLLTGDRGLAGAFSSNIVRFAA
                     EKFDKYPVPVDYVAVGRKGRDLLYRRGKSVIAEFSNLPPAPTFGDVSAIGRVAVDEFL
                     SGKVDEVYLLYTDYINMARQVPDLKKLLPLEVTGGEGRVQDYQPAHIGPAATYIYEPD
                     EMAILDEIIPRFTSLQVYQAILESLASEHAARMVAMKNATDSASALADALLLEYNNVR
                     QQGITNEILDIVGGAEALGSS"
     gene            complement(3491..>3752)
                     /locus_tag="VN363_01030"
     CDS             complement(3491..>3752)
                     /locus_tag="VN363_01030"
                     /inference="COORDINATES: protein motif:HMM:NF012527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit alpha"
                     /protein_id="HWQ83114.1"
                     /translation="LQEILKQPQYVPMSLENMVIELFAGTRGFTDNVPLERMGAWETA
                     MLRFMESNYPEIVKDIATRKQIIPETEQKLRAALDSFMSTWS"
BASE COUNT          795 a         1095 c         1076 g          786 t
ORIGIN      
        1 gatcttaacc tgttccgcgg taagctgtcc gatgatcacc ccatatttct tacggacata
       61 attaacaatg gattcgtcca tcgccatgcc gcctgagcgc atcgtctcgg cggtgacgat
      121 cccgtacatc gccaggactg ccgcctgggt cgccccaccg cccaggcaca ggatcatatt
      181 gcccgaaggt gtgctgatcg ggagatcgat ccccagggcg gctgccagag gctgggggat
      241 caggttgaca tcccggctgc cggcgttcaa agcggcttcc tgcacggcgc gcgattctac
      301 gctggtcacc ccataaggaa ccgtgatcag gatgcggggt gggaacatgc ggttggagat
      361 cttccggagc agaaagcgca gcatatgttc ggtgacttcg taatcggcga tcaccccatt
      421 caacatgggc cgggttacct ccatggaatc aggtacccgt ccgaacatat caaaagcctc
      481 ctgcccccag gcgaccattt tttgttcatc gacgacaatg gcaacaacgg taggctcttc
      541 aatcagcagc tgattgccag agacgacgcg cgtgtacatc gtccccaaat cgatgccaag
      601 atcttttgaa aataaagcca taaaggagag ctcttagttc tcttttgaag cgccagaacg
      661 cccatgcgcc tggcggtagc gctgggcagc ttccaggcgc aggttcgacc ggcgcagggc
      721 agcttccatg gccaggtaag cgtcggtatc cggcggtggt cctttggcca gcagctcttc
      781 cgcccggcgg cgagattgtt ccgcccgggc aagatcaatc tcattcacat tctcggctgc
      841 gtttgccagg accgtgatga tatcgggctg aatttcggca atcccgccag caatggtgaa
      901 gtactgttcc tcgcccttat agcggacttt caggataccc agggtcaagg tcgtcagtaa
      961 aggcgcgtgg ttggggagaa tacccatctc gccttctaca ccgggtagaa caacgatatc
     1021 cgcatcgcct tcgaagacca tgcggtcctg agaaacaatt tcacagcgta tcggcataat
     1081 ttcctcggtg ttccaggcgg aggttcacca ctcaggcagc cgccatctgg cgggagcgct
     1141 cgacgacctc atcgatatcc ccggccataa cgaaagcctg ttcgggaaca tcgtcatgct
     1201 tgccatccag gatttcacgg aaaccgcgca cggtatcctt caggggcaca tagcggccct
     1261 cgcggccggt gaactgttgg gcaacaaaca ttggctggga gaagaagcgc tccatcttgc
     1321 gcgcccggga gacaatcagc ttgtcgtctt cgctgagctc gtccacgccc agaatggcga
     1381 tgatgtcctg gaggtccttg tagcgctgca gcatttgctg gacctcacgg gcggtattgt
     1441 agtggtcaat gccgacaatg ttgggatcga ggatgcgcga agtggacgac agcggatcca
     1501 cagcgggata aatacctttt tcagcgatcg accgctccaa agcaatggtc gcatccaggt
     1561 gggtaaaggt cgccaccggg gctggatcgg agtaatcatc agccgggacg taaactgcct
     1621 gcatcgaggt gatcgagccg ccgcgggtgg aggtaatccg ttcctgcagc tcgcccatct
     1681 ccgtagccag ggtgggctgg taaccgactg cggagggcat gcggcccagc agcgccgaga
     1741 cttcggagcc ggacatcgag aaacggaaga tattatcgat gaagagcagc acatcttttc
     1801 cagaatcgcg gaagtattcc gccatcgaga gcgcggagag cgccacgcgc aaacgcacac
     1861 cggggggctc gttcatctga ccgaagacca tcacggtatc tttcataacc cccgattcga
     1921 gcatttcacg gtaaagctga gtgccttcac gggtgcgctc acccaccccg gcaaacacag
     1981 agttgccctg gtggacggaa gcaatggagc gaattaattc catgatgatg acagtcttgc
     2041 cgacgcccgc gccgccgaag atgcccgtct taccgccttt ggtgaagggc gcaatcagat
     2101 cgatgacctt cactccggtt tcaaagactt caacccgggt ggactgctct gagaaagggg
     2161 gtgcaaggcg gtgaatcggc aggtactcgt ccgtctcaac cggacccaat ccatccacag
     2221 gctgcccgag gacattgaaa acccgcccca gggtagccgg gcctaccggc actttgatcg
     2281 ccgcgccggt agcatacgcc gggcgcccac gctgcaagcc atcggtggca tccatcgcca
     2341 cggtgcggac gacattatcg cccatatgct tttcgacttc cagaattaaa gtcttgccac
     2401 cctccatggg aacactgatt gcttcgtaaa tctctggcat tgcctcggct gggaattcaa
     2461 catctaccac accgcctaaa atttgcacaa catgcccggt tgcatttgcc attctctcgc
     2521 ctccgaaaaa aaataattgc tcgctgcgcg atttagtatc gatcaggatg agccgagcgc
     2581 ttctgcgccg cccacgatgt cgaggatttc gttggtgatc ccctgctggc gaacattgtt
     2641 atattccagg agcagtgcgt ctgccagggc agaggcgctg tcggtggcgt tcttcatcgc
     2701 caccatacgc gcagcgtgct cgctggccag ggattccaaa atggcctggt atacctgcag
     2761 agaagtaaaa cgggggatga tctcatccag gatcgccatc tcgtctggtt cgtagatata
     2821 cgtggcagcc gggccgatgt gggccggctg gtagtcctgc acgcgacctt cacctccggt
     2881 aacctccagc ggaagcagct tcttcaagtc cggcacctgg cgcgccatgt tgatgtaatc
     2941 ggtgtacagc aggtatactt cgtcgacttt gccggacagg aattcgtcca ccgcaacccg
     3001 gccaattgcc gacacatccc caaaggtggg ggcgggtggg aggttgctga attccgcgat
     3061 caccgatttc ccgcggcggt agagcaggtc gcggcctttg cgccctacgg cgacataatc
     3121 caccgggacc ggatatttat caaatttctc ggcggcaaag cgcacgatat tggagctgaa
     3181 ggcgcctgcc aggccgcgat caccggtcag caggacaacc agcacacgat tgacgctggg
     3241 gcgctgaacc agcaatgggt gcatctgctc gcgccctggc tgccgggcga tatgggtgag
     3301 cacctgccag gctttcgtgg catagggccg ggtacgcagc aaggcttccg ttgacttgcg
     3361 cactttgctg gcgctgaccg cctgcattgc ccgcgtcacg tgggagatat tttttacgct
     3421 ccggatgcgg agcttgactt ctctagcatt tgccatggca gagtcctgtt cagcagcgga
     3481 tgaggttcga ctagctccag gtgctcataa acgaatcgag cgcagcgcgt aatttctgct
     3541 cggtctcagg aatgatctgc ttgcgggtgg caatatcctt gacgatctcc ggataattgc
     3601 tctccatgaa gcgcagcatt gcagtttccc aggcgcccat gcgttccaga ggcacattat
     3661 cggtgaagcc gcgcgtcccg gcaaacagct cgatgaccat attttccagc gacatcggca
     3721 cgtattgagg ctgcttgagg atttcttgga gg
//
LOCUS       DATMXK010000052         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_144915, whole genome shotgun sequence.
ACCESSION   DATMXK010000052 DATMXK010000000
VERSION     DATMXK010000052.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144915"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VN363_01035"
     CDS             complement(<1..1134)
                     /locus_tag="VN363_01035"
                     /inference="COORDINATES: protein motif:HMM:NF016800.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-antigen ligase family protein"
                     /protein_id="HWQ83115.1"
                     /translation="MNKNAVATLPITASVANQNFIFLVLAWICGIIVWFNPGVGVWFL
                     LPVFLLWIVRGIKTHQFIQSSLLNLPGAVFMLTAIIGVWTAYDFQAAFHKFLILLAGI
                     FIYIALAYLDQERIFWFFGLVSVTALLLAGYFLLSNDWAAIPADFDLINRLAERWMAV
                     RAGLPPGFDNANIPAGILAVMLPLIAAFTWYAQANLRHHWRWIGWVSFSVVYFAIFMS
                     SSRAVWMALAAGLAGWAWWEISNRAGHRLGWNPRKLFLMGISIVVAAGVFLLSYQASQ
                     FNSVASVLTGGSSAFIRSNLYHQTTDLIADFWLTGGGLQSFAGLYSKYILDIPYLFFK
                     YAHNLWLDVALEQGLFGLLSFVAIYAWTSIQLLHFIVRSSQDLK"
     gene            complement(1127..>2051)
                     /locus_tag="VN363_01040"
     CDS             complement(1127..>2051)
                     /locus_tag="VN363_01040"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559987.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ83116.1"
                     /translation="LLPLDLYRMPLIKGPFTFILTSRGCPAGCTYCIKHVSYQYTTRL
                     RSVDLIMEELWKLKELGINNIHMYSDLFTVNREQVMDLCQRMIDEKINLKWTSNSRVD
                     YVDEEMLRMMGKAGNHLISWGIESGNEQILKHAHKGAYPDKAERALRWARDAGIKNWG
                     YFIIGLPGETEATIRQTIDFAKKLPLDIALFHVAAPYPGTPFFFEVVENGWFRPGTRW
                     EQVDMDKGTVLDYPDLPAEKLLYWQKRAFREWAFRPGPMLTYLKMLVSDWSTVKSAIT
                     VGLAHLSWATTDSGAPIAGELAGNLGVKLRE"
BASE COUNT          558 a          550 c          501 g          442 t
ORIGIN      
        1 tttgagatcc tgagatgagc gaactataaa atggagcagc tggatgctcg tccatgcata
       61 aatcgccacg aatgaaagga gcccaaagag gccctgttcc agagccacat ccagccacag
      121 attgtgggcg tacttaaaaa atagataagg gatatcaaga atatatttag aatagagacc
      181 agcaaatgac tgcagtccac cgccggtaag ccagaaatcg gcgatgagat ccgtcgtctg
      241 gtggtaaaga tttgacctga tgaaagcgct tgagccgccg gttaaaactg acgcaaccga
      301 attgaactgg gatgcttgat agcttaataa gaacaccccg gcagcaacga caatactgat
      361 ccccatcagg aataatttac gcggattcca tcccaatcta tggccagcac ggttacttat
      421 ctcccaccat gcccagcctg ccaatcctgc cgcgagtgcc atccataccg cccgtgagct
      481 gctcatgaag atggcgaaat atacaacgct aaaactgacc catccgatcc aacgccaatg
      541 atggcggagg ttagcctgag cataccaggt aaatgctgcg atcagtggca gcatcaccgc
      601 taaaattccg gccgggatat tggcgttatc gaaaccgggt ggcaaccctg cccggacagc
      661 catccagcgt tctgcaaggc gattgatcag atcaaaatct gctggtatag ccgcccaatc
      721 attggacagc agaaaatagc cagccagcag taatgctgtc acgctgacca ggccaaaaaa
      781 ccagaatatc cgctcctggt ccaggtaggc cagggcaata tagataaaaa tccctgccag
      841 caagattaag aatttgtgga aggctgcctg aaaatcgtat gcagtccaca ctccgatgat
      901 agcggtaagc atgaaaaccg ctccaggcag attaagcaga ctgctctgga taaactggtg
      961 ggttttaatt ccccgtacta tccagagcag aaagaccggc agcaaaaacc agacacccac
     1021 tcccggattg aaccagacga tgatcccaca gatccaggcc aggaccagga atatgaaatt
     1081 ctggttggcc acgctcgcag ttatcgggag agtggccact gcgttcttat tcacgcagct
     1141 tgacacccag attaccagcc agctctccgg cgatcggtgc gcctgaatcc gttgtcgccc
     1201 agctgagatg tgcaaggcca actgtgatgg cagacttgac cgtggaccaa tcggatacca
     1261 gcattttcaa atatgtgagc atcggacctg gacggaaagc ccattcccga aaggcgcgct
     1321 tctgccagta aagtaacttt tccgccggga gatctggata gtcgagcacg gtgcctttat
     1381 ccatatcgac ctgttcccaa cgtgtacccg gtcggaacca gccattttca acgacttcaa
     1441 agaaaaacgg cgtaccagga tacggtgcag ctacatggaa caaagcgata tcgaggggta
     1501 atttcttggc aaaatcgatc gtctggcgga tagtcgcttc cgtttcgcct ggtaagccga
     1561 tgatgaagta accccagttt ttaatccctg catcacgcgc ccagcgcaag gctcgctcag
     1621 ccttgtcggg ataagcccct ttatgggcat gtttgaggat ctgctcattg ccactctcga
     1681 tcccccatga gattaaatgg ttgccggctt tccccatcat gcgcagcatt tcctcatcta
     1741 cataatccac ccggctgttg cttgtccact tcaagttgat cttctcatcg atcatccgct
     1801 ggcacaggtc catcacctgc tcgcgattca cagtaaacag gtccgaatac atatggatat
     1861 tgttaattcc caattctttc agcttccaca gctcttccat gatcaggtcg acggagcgta
     1921 agcgagttgt gtactggtag ctgacatgct tgatgcagta ggtacagcct gcagggcagc
     1981 cccgggaggt gagaataaag gtaaacgggc ctttaatgag cggcatccgg taaagatcca
     2041 gcggaagcag c
//
LOCUS       DATMXK010000053         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_78305, whole genome shotgun sequence.
ACCESSION   DATMXK010000053 DATMXK010000000
VERSION     DATMXK010000053.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_78305"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..934
                     /locus_tag="VN363_01045"
     CDS             <1..934
                     /locus_tag="VN363_01045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560416.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HWQ83117.1"
                     /translation="HPELTGRENISLNGAILGMKRKEIEQKYDEIVDFSGVEKFIDTP
                     VKRYSSGMYLRLAFAVAAHMEPEILVVDEVLAVGDAEFQRKCLGKMSDVAHEGRTVLF
                     VSHNMSAILRLTEETIVLEKGRVAYRAPTSEAVDYYLTSGFAQTGERCWHADEIPAEA
                     APFKPLALRVCNPQGTIAETLRSIEPCTVEMEYELSAPITGLRVGIYLMSTHGDQIFS
                     SFDTDNADEYNRYMIRPAGRFISRCTIPADVLNDGRYILGVNASAFRVKRYFQDEYAV
                     SFTVDSTGAPGMQWAEPRPGPIRPRLDWDIEVIR"
     gene            934..>2170
                     /locus_tag="VN363_01050"
     CDS             934..>2170
                     /locus_tag="VN363_01050"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83118.1"
                     /translation="MALNKKAIKEVLGEVPLAAELYWQIRQQGRPISKSGSLRRTQKH
                     LLDWVAQATAARALHPSGQRVMLFSTLRYWIEYNTLLAVSLAGLGHQVSHTYLPYTSW
                     QKRINHFDLRRQNAYIQSILNPCAPLLNPVSLLELKPSSAPLPDQIAQGVKDVSLRDV
                     QYSLQLEDVELDNPTTPSGQLYQLRLERNTRAALQFITWLHAHRPDVIITPNGMILEM
                     GVLYQVASALDLPVVTYEFGEQRDRIWLARNAEVMRQDTNDLWAARRDQPLDASQWQQ
                     VQELFASRQRASLWENFSRRWQGQPGVGGERVRQELGLNERPVILLAANVIGDSLTLG
                     RQVFSASMTEWLQRTARYFVNRPDVQLVIRIHPGERYTKGPSVADVVRQALPEMPAHF
                     RLVGANDPINTYDLVEIADL"
BASE COUNT          477 a          605 c          615 g          473 t
ORIGIN      
        1 tcaccccgag ctgaccggcc gggagaatat ttccttaaac ggcgccattc tgggcatgaa
       61 acgcaaagag atcgaacaga agtatgatga aattgttgat ttctctggtg tggaaaaatt
      121 catcgataca ccggtcaagc gctattcgag cggtatgtat ttgcgcctgg catttgctgt
      181 agccgcccat atggaacctg aaatcctggt tgtagacgaa gtcctggcag taggggatgc
      241 tgaattccag cgtaaatgcc tgggcaagat gagcgacgta gcccatgagg ggcgcacggt
      301 cctgtttgtc agccacaata tgtctgcaat cctgcgcctg acggaagaga caatcgtcct
      361 cgaaaaagga cgggtggcct accgggcgcc caccagtgaa gcggttgact attatttgac
      421 ttccgggttt gcccaaaccg gcgagcgctg ctggcatgcg gatgaaatac ctgctgaagc
      481 agcccctttc aagccgctcg ccctgagggt gtgtaatcct cagggcacga ttgctgaaac
      541 tctgcgctcg atcgaacctt gcacggtcga gatggaatat gagctgtctg caccgatcac
      601 aggtttgcga gttgggattt acctaatgtc cacccacggc gatcaaatct tcagcagctt
      661 cgataccgat aacgcggatg aatacaaccg ttatatgatc cgcccagcag gtcgatttat
      721 cagccggtgc acaatccctg ccgatgttct caatgatggg cgttacattc tgggtgtgaa
      781 cgccagcgcc ttccgggtga agcgttattt ccaggacgaa tatgccgtat cctttacggt
      841 cgattcaacc ggtgcgccag gcatgcagtg ggcagaaccc cgccctggac cgatccggcc
      901 gcgcctggat tgggatatcg aggtgattcg ctgatggcgt tgaacaagaa agcgatcaag
      961 gaggttttag gcgaggttcc gctggctgcg gagctctact ggcagattcg ccagcaaggg
     1021 cgcccgatca gtaaaagcgg ctccttacgc aggacccaaa aacacctcct ggattgggta
     1081 gctcaagcca cagccgcccg tgccttgcac ccgtccgggc agcgggtgat gcttttcagc
     1141 actctgcgct attggatcga atacaataca ctgttggctg tcagcctggc cggactaggg
     1201 caccaggttt cgcataccta cctgccttat accagctggc aaaaacggat taaccacttt
     1261 gatctgcgcc gccaaaatgc ctacatccag agcatcttaa acccatgcgc gcctctcctg
     1321 aatcctgtct ctttattgga gcttaagccg tcttcagcgc ctctgccaga tcagatcgct
     1381 caaggtgtta aagatgtgtc actgagggat gttcaataca gcctgcagtt agaagatgta
     1441 gagctggata acccgacgac gccgtccggt cagctgtacc agctccgtct ggaacgcaat
     1501 accagggctg ccctgcagtt catcacctgg ttacacgccc atcgaccgga tgtcatcatt
     1561 actccgaatg ggatgatcct ggagatgggt gttctgtacc aggtagccag cgcattggat
     1621 ttaccggtcg tcacttacga gttcggtgaa cagcgcgacc ggatctggct ggcccgtaac
     1681 gctgaagtca tgcgccagga taccaacgat ttatgggctg cccgacgcga ccaaccactc
     1741 gacgccagtc agtggcagca ggtccaggag ctgtttgctt cccgccaacg ggccagcctg
     1801 tgggagaatt tttcacgccg ctggcagggc caacccggcg ttggcggaga gcgggtgcgc
     1861 caggagctgg gtctgaatga acgcccggtc atcctgctgg ccgcgaatgt aattggcgac
     1921 agcctgacac ttggtaggca ggttttcagc gccagtatga ccgaatggct gcagcggact
     1981 gctcgctatt ttgtcaaccg gcctgacgtg cagctggtga ttcgcatcca ccccggcgag
     2041 cgctacacaa agggcccttc ggtggcagac gtcgtcaggc aggcgctgcc tgagatgccg
     2101 gctcacttcc gcctggtagg ggcgaatgac cccatcaaca cttatgatct ggtggaaatc
     2161 gccgacctgg
//
LOCUS       DATMXK010000054         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_87530, whole genome shotgun sequence.
ACCESSION   DATMXK010000054 DATMXK010000000
VERSION     DATMXK010000054.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87530"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2287
                     /locus_tag="VN363_01055"
     CDS             <1..>2287
                     /locus_tag="VN363_01055"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWQ83119.1"
                     /translation="LLQLTPNPREHFQALEMNFLSTASGVELINGLEALPALYRGWLS
                     GLKLDEVPAGLQDAARQNLHNCEVVANRIEHGIQLIKEDEQVREAFQLANKAMLIQRG
                     RVTWLKMPADKRPPVPVLDESHTWRPFQLGFILLCLPSIANPEIPDRQLVDLLWFPTG
                     GGKTEAYLGLTAFTIFLRRMRGAGRKITAGVTVLMRYTLRLLTIQQFQRASTLIMACE
                     TIRRQSKGKLGEEEINLGLWVGGAATPNRLSQAKTALEQLLQGERVLEGNPYQIHNCP
                     WCGTPITPRHYRIGVTLVIHCPNEGCEFATALPLYLVDEDIYRRRPSLLIGTVDKFAR
                     LPWLAEAGAIFGRPDGETLPPELIIQDELHLISGPLGSLVGLYETAIDRLCQQNGSPP
                     KIIASTATIRRAAEQSKGLFNRGLGQFPPPALDARDNFFSWQISPDVRPGRLYAGVHA
                     PGKSMKTALLRIYAVLLQRIFEHTAEPALRDPYWTLVGYFNSLRELGGALRLVEDDVR
                     DRMKLLASRAGVKQRYINYYRELNSRIGSDEIPEILDHMSKSINAHSAIDVVLATNMI
                     SVGMDIDRLGLMVVNGQPKMTSEYIQATSRIGRQYPGLVVTLYNWTRPRDRSHYEGFV
                     PYHSSIYSQVEPTSVTPYSNRARDRGLAGVFITLMRHLYSDLNPEQAAGNFDPISPDA
                     NEVIEAILARVSEIDPVEVDEVRQELEYIRSRWGELSMADNLTYGRSYKNPALPHLMD
                     PVEEVHGADILSFPTLNSLRDV"
BASE COUNT          576 a          573 c          572 g          566 t
ORIGIN      
        1 agctattaca gctcacacct aatcctcgag agcattttca ggcacttgaa atgaactttc
       61 tatcgactgc ctccggagta gaattgataa atggattgga ggcattacct gccttgtatc
      121 gcggctggtt gagcggacta aaactggatg aggtgcctgc tggacttcag gatgccgcca
      181 gacagaatct gcacaattgt gaggtagttg ccaatcgcat cgaacatgga atccagttaa
      241 taaaagaaga cgaacaggtt cgagaagcat ttcaacttgc caataaggcc atgctaatcc
      301 aacgcggcag ggtgacctgg ctgaaaatgc cggctgataa gcgcccgcct gttccagttc
      361 ttgatgaatc ccatacctgg agaccctttc aattagggtt tatccttctg tgcctgcctt
      421 ccattgccaa tccagaaatt cctgaccgcc agcttgtcga tttgctctgg tttccaacgg
      481 gaggcggaaa gactgaagct tacctcggac ttaccgcttt taccatcttc ctgcggagga
      541 tgagaggggc tggacggaag attacagcag gtgtcacggt gttgatgcgg tatacattgc
      601 gattgctgac catccagcaa tttcaacgtg catccacact gatcatggcc tgtgaaacaa
      661 ttcgtcgcca aagcaaggga aaactgggag aagaggagat caatcttggt ctgtgggtcg
      721 gtggcgctgc cacaccgaat agactcagcc aggcaaaaac agctctggag cagttattgc
      781 agggggaacg tgttctggaa ggcaatccat accagatcca taactgtccc tggtgtggaa
      841 cgcctatcac acctcgtcac tatcgaatcg gtgtaactct ggtgattcac tgtcccaatg
      901 agggttgtga attcgcaaca gcgctgcctt tataccttgt ggacgaagat atctaccgac
      961 gcaggccttc gcttcttatc ggcactgtag ataaatttgc tcgtctgcca tggctggcag
     1021 aagcaggtgc gatttttggc agaccagatg gtgaaacatt gcctcccgaa ttaatcattc
     1081 aggacgaact ccaccttatc tcgggacctt taggatcact cgtcgggttg tatgaaactg
     1141 ccattgaccg tttgtgccag cagaatggta gcccacccaa aattatcgct tccacagcca
     1201 caatccgtcg cgcggcagaa caaagcaaag gactctttaa tcgaggactt ggccagtttc
     1261 ctcctcctgc actggatgcc cgagacaatt tcttttcctg gcagatatcc ccagatgtca
     1321 gaccaggacg gctttatgca ggtgtacatg cacccgggaa aagcatgaaa accgccctgc
     1381 tacgtattta tgctgtgctg cttcaaagga tattcgaaca cacggcagaa ccagctctac
     1441 gtgatccata ctggacactg gtgggctatt tcaacagttt gagagagctg ggcggtgctc
     1501 tgcgcctggt tgaggacgac gtccgggatc gtatgaaact gctcgcctcg agggcggggg
     1561 tgaaacagag atatatcaat tattaccggg aactgaacag tcgtattgga tcggacgaaa
     1621 tcccagagat cctggaccac atgtcaaaat ccataaacgc ccattcagcc atcgatgtcg
     1681 tgcttgcaac caatatgatc tccgttggga tggatattga ccgtcttgga ttaatggtgg
     1741 ttaacgggca gcctaagatg acatcagaat atattcaggc taccagccgg attggccgcc
     1801 agtaccccgg actggtggtc acgctataca actggacaag accccgtgat cgctctcatt
     1861 atgagggatt cgtcccctat cacagctcaa tctacagtca ggttgaaccg acttcagtaa
     1921 ctccgtattc aaatcgagca cgcgatcgtg ggttggctgg tgtctttatc actttgatgc
     1981 gccacctgta ttctgatctc aatccagaac aggcagccgg aaactttgat ccgatatctc
     2041 cagatgcaaa tgaggtcatt gaagcaatcc tggccagagt gagtgaaatt gatccggttg
     2101 aagtggatga agtccgtcag gaactggagt atattcgttc tcgttgggga gagctgtcca
     2161 tggcagataa tcttacctat gggcgctcct acaagaaccc tgctctcccc catcttatgg
     2221 atccggttga agaagttcat ggagctgata ttctctcctt cccaacgcta aactctcttc
     2281 gtgatgt
//
LOCUS       DATMXK010000055         2447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_99537, whole genome shotgun sequence.
ACCESSION   DATMXK010000055 DATMXK010000000
VERSION     DATMXK010000055.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99537"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..260
                     /locus_tag="VN363_01060"
     CDS             <1..260
                     /locus_tag="VN363_01060"
                     /inference="COORDINATES: protein motif:HMM:NF012267.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWQ83120.1"
                     /translation="IDIDVCMNCGLCAEYCPFDAIKMDHDYELSVYQRHEKNIVDKER
                     LSKPESYYASIRPVNYASEEQAKAEAEAAKAARKAKPAEAG"
     gene            281..1369
                     /locus_tag="VN363_01065"
     CDS             281..1369
                     /locus_tag="VN363_01065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560223.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0140663 - ATP-dependent FeS chaperone
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWQ83121.1"
                     /translation="MMNNHQHANQEAVLAALSKVQEPELHKDLVTLNMVRDINIDGKK
                     VSFKVVLTTPACPLKTRIEREAREAVLAIEGVESVEIQMDSNVPSDGRSRGLLDLPIR
                     NAIAVGSGKGGVGKSTVAVNIAVVLAQSGARVGLLDADIYGPNVPTMMGVQHLPQPKE
                     QKLIPAEAYGVKIMSIGFLVKPDQPLIWRGPMLHSAIRQFLTDVTWGELDYLIVDLPP
                     GTGDAALSLAQSLPLSGSVIVTLPQQVSLEDARRGLEMFRQLEVKIFGIIENMSYLEL
                     PDGTRMDIFGQGGGQKLAEQSGVPFIGSIPMDPNVRTGGDNGVPVVISNPESPVAKAL
                     HEIAENIAAKISVSAVSQGNFIPINLIG"
     gene            1430..>2447
                     /gene="ricT"
                     /locus_tag="VN363_01070"
     CDS             1430..>2447
                     /gene="ricT"
                     /locus_tag="VN363_01070"
                     /inference="COORDINATES: protein motif:HMM:NF041131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="regulatory iron-sulfur-containing complex
                     subunit RicT"
                     /protein_id="HWQ83122.1"
                     /translation="MTQPYIVGIRFQKTGKVYHFDASQQADLIVGDYAVVDTSRGRQL
                     GEIVQIVPEPTSPPEGSWKAITRRATPRDLVLRQLWQKKEIEAMINCRERLAEINLPG
                     VKIVAAEFSFDGSGLTFLYSTEAEGKIDLRDLRRVMQRIYPRARVEMRQIGPRDVAKI
                     LGGMGACGLDNRCCSMFLTDFSPISIKMAKEQGISLSPTEITGMCGRLRCCLVYEYEQ
                     YVEARKTLPKRNKRVITPLGEGKVNDVLPLKQTVLVYLDTGVVQEFPAQDLQPWDELE
                     ALRRKSQEPCDRHENGGCNCGKSTPTAAGSTEAEEIEPDEPDLRRPPTSTPPAENRNI
                     DQTSR"
BASE COUNT          617 a          627 c          678 g          525 t
ORIGIN      
        1 atattgacat tgatgtgtgc atgaattgtg gattgtgtgc ggaatactgc ccctttgatg
       61 ccatcaagat ggaccacgat tacgagttat cggtctacca gcggcatgag aagaatatcg
      121 tggataaaga gaggctatcg aagccagaat cgtattatgc ttcaatccgc ccggttaact
      181 acgccagcga agaacaggca aaagccgagg ctgaagctgc gaaagctgct cgaaaagcaa
      241 aacctgctga agcgggttaa ttattaattg agaaaggtaa atgatgaata atcaccagca
      301 tgccaatcag gaagcagtcc tggcagccct gagcaaagtt caggagcctg agctgcataa
      361 agacctggtt accctcaata tggtccggga tatcaatatt gacggtaaaa aagtcagttt
      421 caaggtggta ttaacgactc ccgcctgtcc cttgaagacc cgcatcgaac gggaggcacg
      481 cgaagctgta ttagcgatcg agggtgtgga aagcgttgag atccagatgg attccaatgt
      541 tcccagcgac gggcgttcac gcggtctgct cgatctgcca atccggaacg ccatcgcggt
      601 tggatccggt aaaggtggcg tgggtaaaag caccgtagca gtcaatattg ccgttgtatt
      661 ggcgcagagc ggtgcgcgag ttggcctgct ggatgcagat atttatggtc ccaacgtacc
      721 caccatgatg ggcgttcaac acctaccgca gccaaaagaa caaaagctga tccccgctga
      781 agcctatggg gtcaagatca tgtcaatcgg ctttctggta aaacccgacc aacccttgat
      841 ctggcgtgga cctatgctcc attcggctat tcgccagttt ctgacggatg taacctgggg
      901 cgagctggat tatctgattg tcgatctccc gccgggaacc ggcgatgcag ccttaagcct
      961 ggcgcagtcg ctgccgttga gcggaagtgt aatcgtcacc ctcccccagc aggtatctct
     1021 cgaagatgcg cgcagaggat tggagatgtt ccgccagctg gaggtcaaaa tcttcggcat
     1081 tatcgagaat atgagctacc tggagctgcc ggatgggacg cgtatggata tttttggcca
     1141 gggcgggggc caaaagctgg ccgaacaatc cggggttccc tttatcggga gtattccgat
     1201 ggatccgaat gtccggactg gtggagataa tggggttccg gttgtcatca gcaaccccga
     1261 atcaccggta gccaaagccc tgcatgagat tgctgaaaac atcgccgcca agatcagcgt
     1321 ttcggcggtc agccagggca atttcatacc gatcaacctg atcggatagc aaatcccttt
     1381 acgccagcct ggcaaccttt ccggaggcct ggctggggcg aaaaatctta tgacccaacc
     1441 atacattgtc ggtatccgtt ttcagaaaac aggtaaggtc tatcatttcg acgccagcca
     1501 gcaagcagat ctgattgtgg gtgattatgc cgttgtagat acaagccgcg gtcgccagct
     1561 gggtgagatc gttcagatcg tccctgaacc cacctcaccc ccagaaggga gctggaaggc
     1621 tatcactcgc cgggcaacgc cgcgcgatct ggtattacgc cagctctggc aaaagaaaga
     1681 aatcgaggcg atgatcaact gtcgcgaacg cctggcggag atcaacctgc cgggtgtaaa
     1741 aattgtggca gcagaatttt cgttcgatgg gtcagggctt accttcctgt acagcactga
     1801 agctgaaggc aagatcgatt tgcgcgactt aaggcgggtc atgcagcgaa tttacccacg
     1861 ggctcgcgtg gaaatgcgcc agatcggtcc acgggatgtc gctaaaatcc tgggagggat
     1921 gggcgcctgc gggttagaca accgctgctg ttccatgttc ctgaccgatt tcagcccaat
     1981 ttcgatcaag atggccaagg aacagggtat ctcgctctcc ccaaccgaaa tcacagggat
     2041 gtgcggacgc ttacgctgct gcctggttta tgaatacgag cagtacgtcg aagctcgcaa
     2101 gacccttcct aagcggaata aacgcgtgat cactcccctc ggtgaaggta aggtcaatga
     2161 tgttttgccc ctgaaacaaa ccgtgctggt gtatctggat accggggtcg ttcaggagtt
     2221 tcctgcccaa gatttacaac cctgggatga actcgaagct ttgcgtcgaa aatcccagga
     2281 accttgcgac cggcacgaga atggtgggtg taactgtgga aaatctaccc ccacggcagc
     2341 tggctctacc gaagctgagg aaattgaacc agatgagcca gatctacgac gaccacccac
     2401 cagtactccg ccggctgaaa acaggaatat cgaccagacc tcacgcc
//
LOCUS       DATMXK010000056         3545 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_157428, whole genome shotgun sequence.
ACCESSION   DATMXK010000056 DATMXK010000000
VERSION     DATMXK010000056.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3545)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3545)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3545
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157428"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1454
                     /locus_tag="VN363_01075"
     CDS             <1..1454
                     /locus_tag="VN363_01075"
                     /inference="COORDINATES: protein motif:HMM:NF024640.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prenyltransferase/squalene oxidase
                     repeat-containing protein"
                     /protein_id="HWQ83123.1"
                     /translation="IWLVVGLITILVFLIVILNFLRPDETLDTQSTAAAAAMTETAIS
                     VMPAAETGVPETSTPEINPTATTRVDPTATEAPAQAASRAYVAVEPADGSLILSPITF
                     TAPITGLDALLQTGLDIVTQETSFGTAVCSIEGVGCPADNCFCSATNFWNYLNWDWAQ
                     NQWVSSAVGASATTVANYAFDGWRWQVFEDTLTPPPVPQLVTSSAMSYLLGRQDPATG
                     GYGSLGSTLDFLLAAGASGTPVSDLKAPDGAQSLQEYVILEGAAYAESDAGTAGKLAM
                     GLAAAQMCKPADTKSPQDYYDPATGVYSNQSLFQAFAMLGVLASGESVSPEAVAYLNS
                     QALPSGGWAWFPGETEDSNTTAIALQALIASGEPVTSPVIINGLTFLQSFQNADGGFS
                     YSQEYLGVSDSNSTAYVIQAIYAAGQNPVTEAWTKDSRNPIFYLLDLQQEDGSFEWQA
                     GSGPNDLATMQAVVALLSRPYPISTAGFAECAQ"
     gene            1508..2203
                     /locus_tag="VN363_01080"
     CDS             1508..2203
                     /locus_tag="VN363_01080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83124.1"
                     /translation="MLKLRSFLYPLLGGSILVLALALTDLPLAIAQEPERVGLVIQFA
                     DETVVASCIDISGQEPNGYDILQLAGLDLGINSDANQDIAVCSVNGQGCDANHCFCQF
                     PDYWSYWHTEGNEWIYSGRGASTYKVKPGTVEGWRWGSGIPPAPVSFEQICSTSAPDF
                     ISGPTVASEHLSPGVLLKPVNGVDSIPGSRSSSPDASKPVSLQASIGYIVFGFALLGM
                     GVGLFIVAKTERS"
     gene            2200..3027
                     /locus_tag="VN363_01085"
     CDS             2200..3027
                     /locus_tag="VN363_01085"
                     /inference="COORDINATES: protein motif:HMM:NF024231.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ECF transporter S component"
                     /protein_id="HWQ83125.1"
                     /translation="MKNRWLSPIIYLVCSLVGILSLLYPFVLPQVTTGAQSGQQRSAD
                     MPLMLSVLLGLALIVLVYEVQGQGMSTKLIALLGVLVAINAALRFLDIAIPVPGGFSP
                     IFFLIILTGHVYGARFGFLMGVMTLVVSAILTGGVGPWLPGQMFAAGWVGMSAPLLRP
                     LVRRLQLEGRSEEVWILAGFGALWGLFFGVIMNLWTWPFIAGPADQYWTQGAGIGETI
                     RRYASYYLATSLIWDLARAAGNFLIILGFGLPALRALRRFKRRFTFTVAQPPVTMVP"
     gene            3039..>3545
                     /locus_tag="VN363_01090"
     CDS             3039..>3545
                     /locus_tag="VN363_01090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83126.1"
                     /translation="MNTNLHTWTWVAWLATALIALSATRNFYYLILILVCLLVIYFVI
                     EGSVEKSAYPLSMLRFGLMICGFSTVFNLLVSHFGETIFFSLPQAWPLIGGAYTLEAA
                     VYGLTNGMVLFGILIVFTIFSQALPVRKLIRLIPRAFFPVAVVTSIAISFVPTTIRQY
                     HQIKEAQLL"
BASE COUNT          733 a          894 c          969 g          949 t
ORIGIN      
        1 ctatatggct cgtggtcggt ctcatcacca tcctggtatt cttaattgtt attctcaact
       61 tcctgcgtcc tgatgaaacc ctcgataccc aatcgacagc agctgcggcg gcgatgaccg
      121 aaactgccat ttctgttatg ccggcagctg agactggtgt accggaaact tcaactcccg
      181 aaattaaccc aacagctaca acccgggtag atccaactgc gacagaagcg ccagcgcaag
      241 ctgcttcacg cgcatatgtg gccgttgagc ctgccgatgg cagccttatc ctttccccga
      301 ttacatttac agcgccgatc acaggtctgg atgctctgct ccagacaggc ctcgatattg
      361 tcactcaaga aacttccttt gggactgcgg tttgcagcat cgaaggagta ggctgcccgg
      421 ctgataattg tttctgctcg gctaccaact tttggaatta tctgaactgg gattgggcac
      481 aaaaccagtg ggtgtcatcc gcggttggag ccagcgcgac cacagtggca aattatgcct
      541 ttgatggctg gcggtggcag gtatttgagg acacattgac ccctccacct gtaccccagc
      601 tggtcaccag ctccgccatg agctacttgc tgggcaggca agaccctgcg acgggtggat
      661 atggctcgct cgggagcact ttggattttc tgctggcggc tggcgcaagc ggtacaccgg
      721 tgagtgatct gaaagctccg gatggggcgc agagcctgca agagtacgta atactggaag
      781 gcgccgccta tgcagagagc gatgcgggca cggcgggcaa gctggcgatg ggcctggcag
      841 ctgctcagat gtgtaagcca gccgacacga aatcacctca ggattattat gatcccgcca
      901 ccggcgttta cagcaatcaa agcttattcc aggcttttgc catgctgggt gtgcttgcga
      961 gtggcgaatc cgtctcaccc gaagccgtgg cttacctcaa cagtcaggct ttgccgagcg
     1021 gcggttgggc gtggttcccc ggagaaactg aagattccaa tacaacagcg attgccctgc
     1081 aggcattgat tgcctcaggt gagccggtaa cctctccggt tattatcaat ggcctaactt
     1141 ttctacaatc ctttcaaaac gctgatggcg gcttttctta cagccaggaa tacctcggcg
     1201 tgagcgacag taactcgact gcttatgtta ttcaagccat ctatgccgct ggacaaaacc
     1261 cggtaactga ggcctggacc aaagatagcc gtaacccaat tttctatttg ctggatctcc
     1321 agcaggaaga tgggagcttt gaatggcaag ctggcagtgg ccccaacgac ctggcaacga
     1381 tgcaggctgt ggtggccttg ctcagccgcc catacccgat cagcacagct ggttttgctg
     1441 aatgcgccca ataatccagg tcgggatgtc cactgatctg gaagggatcc gataagagta
     1501 aatagcgatg ctgaaactgc gcagcttttt atatccactc cttggtggat cgatcctggt
     1561 cctggcgctg gctctgaccg atctgccgtt ggcgatcgcc caggagcctg agcgcgttgg
     1621 cctggtcatt caattcgccg atgaaacagt cgtcgcaagc tgtattgata tcagcgggca
     1681 ggagccgaat ggctatgaca ttttacagct cgcaggtttg gatctgggca taaattcgga
     1741 tgccaaccaa gatatcgcgg tctgcagtgt aaatggtcag ggatgtgacg ccaaccattg
     1801 cttctgtcag tttccagact actggtctta ttggcatact gaaggaaatg aatggatcta
     1861 ttctgggcgt ggcgcgagta catacaaagt taagcccggc actgtcgaag gctggcgctg
     1921 gggaagtggg attcccccgg cgcctgtttc cttcgagcag atctgttcta cgtccgctcc
     1981 agattttatt tcaggaccta ccgtcgcgag tgagcacctt tcgccgggtg tgttacttaa
     2041 gcctgtgaat ggggtagaca gtataccggg aagcagatca agttcgccag acgcgagtaa
     2101 gccggtttct ctgcaggctt cgattggtta tatcgtcttc ggatttgcgc tgcttggtat
     2161 gggtgtcggt ttatttatcg ttgcgaaaac cgagcgttca tgaaaaaccg ctggttgagc
     2221 cccatcatct atctggtctg cagtctggtt gggatccttt ctctgctcta tccttttgtt
     2281 ctaccgcaag tcactaccgg cgcacaatcg ggtcagcagc gcagtgcgga catgcccctc
     2341 atgctgagtg tgctgctggg gctggcatta attgtgctgg tgtatgaagt tcaaggccag
     2401 ggaatgagca ccaaattaat cgctttattg ggggtgctgg tggcgattaa tgctgccctg
     2461 cgatttctcg atatcgcgat tccagttccg ggaggcttca gtccaatttt ctttttgata
     2521 attttgactg gacacgttta tggtgcccgt tttggttttt taatgggcgt gatgactctg
     2581 gttgtatcgg caatactaac cggtggcgtg gggccgtggc tgccaggcca gatgtttgcc
     2641 gctggctggg ttggaatgag tgcgcctttg ctgcgacccc tcgtccgtcg cttacaactc
     2701 gaaggtcgca gcgaggaggt atggattctg gcaggatttg gcgcgctgtg ggggctgttt
     2761 tttggggtta tcatgaatct atggacatgg ccatttatcg ctggaccagc tgaccaatac
     2821 tggacgcaag gggcaggtat aggcgagaca attcggagat atgcaagtta ttatctggcg
     2881 acatccctga tctgggatct ggctcgcgcc gccggtaatt ttctgataat tttgggtttt
     2941 gggctgccag ccttgcgcgc cttacgccgg tttaaacggc gatttacttt caccgttgct
     3001 cagccgcctg tgacgatggt tccctgagca atttccagat gaatacaaat ttacatacct
     3061 ggacatgggt tgcctggctt gccacagcgc tgatagctct ttcggccaca cgcaatttct
     3121 attacctaat tttgatcctg gtttgcctcc tggttattta tttcgtgatc gaaggctcgg
     3181 tcgagaaaag tgcttatccg ctttccatgc tccgttttgg gctgatgatc tgtgggtttt
     3241 cgacagtatt taatctactg gtctcccatt tcggggagac aatatttttt tcattacccc
     3301 aggcatggcc tctgattggt ggggcttata cccttgaggc agctgtctac ggtctgacga
     3361 acggcatggt tttattcggt attttgatcg ttttcacaat tttcagtcag gcgctgccgg
     3421 tacgcaagct gatccgttta attccacgag ctttctttcc ggtagcagtt gttacatcga
     3481 tagccattag ttttgtccct acaacgatcc gccagtatca ccagattaaa gaggctcaac
     3541 tgtta
//
LOCUS       DATMXK010000057         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_31954, whole genome shotgun sequence.
ACCESSION   DATMXK010000057 DATMXK010000000
VERSION     DATMXK010000057.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31954"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(43..669)
                     /locus_tag="VN363_01095"
     CDS             complement(43..669)
                     /locus_tag="VN363_01095"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWQ83127.1"
                     /translation="MEKVNDRPTPMTLTDFMRQRFKGLLDSIGGFLNGLGLMPNTMTL
                     LGLLGNAIGAYLIARGEMTWGGILILLMGPVDALDGTMARLRGMASEFGAFVDSVTDR
                     YSELVIYGGMLYYFTHQGEVLAGILTFVAASGSVLVSYVRARGQSVGADTKVGILTRM
                     ERYLVLAPALVFQQPLWGLWIIAVLANVTAIQRIFDIRRQLTTPQPDK"
     gene            complement(866..>2166)
                     /locus_tag="VN363_01100"
     CDS             complement(866..>2166)
                     /locus_tag="VN363_01100"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015616 - DNA translocase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DNA translocase FtsK"
                     /protein_id="HWQ83128.1"
                     /translation="VDKAQNGEGEARQKVRVAQISGLSRDLALALSAERLRIEAPVPG
                     RPYVGIEVPNSRSSFVRLRQLLESEAMVKVNSPLAIALGRDVSGSPVVGDLSRMPHLL
                     IAGTTGSGKSVCIAAITACLVMNNTPEDLRLVMVDPKMVELVRFNGLPHLLGKVETEL
                     ERILGVLRWTVAEMDRRYKLLEATHSRNLETYNRKARRRKGEGPLPRIVLLIDELADL
                     MMSAPDQTEHNLVRLAQMARATGIHLIVATQRPSTDVVTGLIKANFPARISFAVASGV
                     DSRVILDTQGAESLLGRGDMLFLAPESSTPIRAQGVWVTDQEIDKIISFWQKSTLPPE
                     EGDLPPWDDLLHKESMLADRDGLLDGAVAIIKKTRRASASLLQRRLHIGYPRAARLID
                     ELAELGYIGPAQPGGREREVLISDDEPDLPSDEDELTLSG"
BASE COUNT          463 a          652 c          620 g          431 t
ORIGIN      
        1 ccttctttct ggaaagaatt ctgagaatga tgaaccggac tattatttat ccggttgtgg
       61 agtcgttaac tggcgccgga tatcgaaaat gcgctggatg gcagtcacat tggccaggac
      121 tgcgataatc cataaacccc acagcggctg ctggaaaacc agtgccggag ccagaaccag
      181 atagcgctcc atgcgggtga gaatccccac tttggtgtct gcacccacag attggccgcg
      241 tgcccggaca tacgaaacca gcacggaacc cgaagcagcc acaaacgtca ggatacccgc
      301 cagaacttct ccctgatggg taaaataata cagcatccca ccatagatta cgagctcaga
      361 atagcgatcc gtgaccgaat cgacaaatgc tccaaattcg ctcgccatac cccgcaggcg
      421 ggccatggtg ccatccagag catccaccgg ccccatgagc aggatcagaa tgccgcccca
      481 ggtcatctcg ccgcgcgcaa tcaggtacgc tccgatcgca ttccccaaca ggccgagcag
      541 ggtcatggta ttgggcatca ggcccaatcc gttcaaaaaa ccaccgatgg agtccagcaa
      601 acctttgaag cgctggcgca tgaaatctgt aagcgtcatt ggcgtgggac gatcattgac
      661 tttttccaat agctggtttt gcctttcatt tcaggtttgt tttcctgtta aacaatacgc
      721 tatgctatcc catgagcgca gggctgtcaa gacaatctga atttcgtccg cttagggggt
      781 gaatcgagtg gcgtgcgtgc actggcgtgc gtgcactcgc gtgcgtgcac tggcgtgcgt
      841 gcacggacgt gagtgggctg atttgctaac ctgagagggt caattcgtct tcgtcagatg
      901 gaaggtcagg ctcgtcgtct gagatcaaga cttcgcgttc gcgacccccg ggttgggctg
      961 gcccaatata gcccagctca gccagctcgt cgatcaggcg ggcagcgcgt ggatagccaa
     1021 tatgcaggcg gcgctgcagg agtgaagcac tggcccggcg ggtcttctta ataatcgcca
     1081 ccgcgccatc gagcagtcca tcccggtcag ccagcatgct ttccttgtga agcagatcat
     1141 cccagggtgg cagatcgcct tcttcaggcg gcagggttga tttctgccag aagctgatga
     1201 ttttatcgat ttcctgatcg gtcacccata ctccctgggc gcgaatcgga gtagaggatt
     1261 cgggtgccag gaacagcata tcgccgcgcc ccagcaggga ttctgcaccc tgggtatcca
     1321 ggataacccg tgaatcgacc ccggaagcca ctgcaaaaga gatccgggcc ggaaaattgg
     1381 ccttgatcag cccggtgaca acatccgtgc tggggcgctg ggtggcgacg atcaggtgaa
     1441 tacccgtagc ccgcgccatc tgcgccaggc gcaccaggtt gtgctcggtc tggtcggggg
     1501 ccgacatcat cagatcagcc agctcgtcga tcagcagcac gatgcgcggc aggggtccct
     1561 cgccctttcg ccggcgagct ttgcgattat aggtttccag gttgcgcgag tgggtcgcct
     1621 ccagcagctt atagcgccgg tccatctctg ctaccgtcca acgcagcacg cccaggatgc
     1681 gttccagctc ggtctcgacc ttgcccaaca gatggggcaa gccgttgaag cgcaccagct
     1741 cgaccatctt ggggtcgacc atcaccagac gcaggtcctc gggggtgtta ttcatcacca
     1801 ggcaggcggt aatcgccgca atacataccg atttaccaga gccagtcgtc ccggcgatca
     1861 gcaggtgcgg catcctgctc aggtcgccaa ccaccggcga gcctgaaaca tcccgcccca
     1921 gggcaattgc caggggtgaa ttgaccttca ccatggcctc cgattccaaa agctggcgca
     1981 ggcgcacaaa gctggaacgc gaattgggga cttcgatgcc cacgtacggg cggccgggaa
     2041 ccggggcttc gatgcgcagg cgttcggcgg agagggccag cgccagatcg cggctcaatc
     2101 cggatatctg ggccacgcgc actttctggc gcgcctcccc ctccccattc tgagccttgt
     2161 ctacaa
//
LOCUS       DATMXK010000058         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_100811, whole genome shotgun sequence.
ACCESSION   DATMXK010000058 DATMXK010000000
VERSION     DATMXK010000058.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_100811"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            160..1179
                     /locus_tag="VN363_01105"
     CDS             160..1179
                     /locus_tag="VN363_01105"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWQ83129.1"
                     /translation="MAETTILVVEDNSLLREGLVTTLESDGLRVLHASNGLEAIEILE
                     QEIPDLILSDIGMPKMDGYALFDHIREHPRWIAIPFIFLTARDDREDIYLSKRLGAED
                     YLVKPVSRLELITTVRARLARNQQLALAQLQQAYQSSLIVMSNAIELRDQYTRGHVER
                     VMAYSVALASQLGFRNGTLNALRLGSILHDIGKIYIRESILRKPGPLNEQEWVEMRQH
                     PIVGADLIRNIPFLSPAIPIIRHHHERWDGSGYPDRLVGEEIPFLARVVAIADSLDAM
                     LIARVYSRPFSPEEARTEIIHLSGKHYDPKLVKAFVQAWPEIFKRIQSAPAAGPLSEI
                     QFAPE"
     gene            complement(1194..2156)
                     /locus_tag="VN363_01110"
     CDS             complement(1194..2156)
                     /locus_tag="VN363_01110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561395.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWQ83130.1"
                     /translation="MEYRYLGHSGLQVSELCLGTMQFGWSADEATSHQILSLAYEAGI
                     NFIDSADVYSRWVEGNPGGVAEEIIGRWIKQSQIPRPKLVIATKVRGKMGNGPNEGGL
                     SRVHIMQAVEDSLRRLGTDYIDLYQAHAYEEHPPIEETLRAFDDLVHQGKVRYIGASN
                     FPAWRLMQALWTSQRDGLARFVSLQPHYHLLNRAEVERELADVCQTYQLGVIPYSPLA
                     GGFLTGKYRQGSEVDSVRARGVRRYFKPENFALLDKMQELGQAKGGYSISQIALAWQL
                     SDPLITSPIIGPRSLEQTQDNLGAAGLRLSEDEKRVLDEATAWK"
BASE COUNT          542 a          686 c          595 g          572 t
ORIGIN      
        1 agattgtaat tatcaaacgg tccgggttat gttagattta atgttgccgg cttcctcggg
       61 ggttctccag actaattgta ctggatattc acttgtagac ggcataattg ttgcatcaat
      121 caagcaatca tcatcacaac gaataaggag ttagagaaca tggccgaaac aaccatcctg
      181 gttgttgaag ataatagctt gctccgtgaa ggactcgtaa ccacgctgga atccgatggt
      241 ttgcgagtac tgcatgcttc caacggcctg gaagccatcg aaatccttga gcaagaaatc
      301 cctgacctga tcttatccga tatcgggatg cctaaaatgg atggttatgc cttgtttgat
      361 catatccgcg agcatccccg ctggattgcc attcccttta tcttcctcac tgcccgtgat
      421 gatcgcgaag atatctactt gagcaagcgt ctgggggcgg aagattatct ggtcaagcca
      481 gtcagccgcc tggagctcat cactacagtg agggcgcgtc tggcccgcaa ccaacagctg
      541 gccttagccc agctccagca agcctaccaa tccagtttga tcgtcatgtc caacgccatc
      601 gagctgcgtg accaatatac ccgtgggcac gttgagcggg tgatggcgta ttcggtcgct
      661 ctcgccagcc agctcggatt tcgaaatggg accctcaatg ccctgcggct ggggagtatt
      721 ctccatgata tcggtaaaat ttatatccgc gaaagcatct tgcgcaagcc aggtcctctg
      781 aatgaacagg aatgggtcga aatgcgccag cacccaattg ttggggcgga tctgatccgg
      841 aatatacctt tcctgtctcc agccataccg atcattcgcc accatcacga gcgctgggat
      901 ggcagtggtt acccggatag actggtcggg gaggagattc ccttcctggc gcgagtggtt
      961 gcgatcgccg attcgctgga tgctatgctc attgcccggg tttattcccg gcctttcagc
     1021 cccgaggaag cccgaactga gatcatccac ctgagtggta agcactacga cccgaaactg
     1081 gttaaggctt tcgtacaggc ctggccagag atctttaagc ggatacaatc agcacctgct
     1141 gcaggtccgc tttccgaaat tcagtttgcg cccgaataat ctctccaaag atcttatttc
     1201 caggcagttg cctcgtccag gacccttttc tcatcttcac tcaagcgtaa acccgctgca
     1261 cccaggttat cctgggtttg ttcaaggctg cgcggcccga tgattgggct ggttatcagc
     1321 ggatcggaaa gctgccatgc cagggcgatc tgcgaaatac tataaccgcc tttcgcctga
     1381 cccaattcct gcattttatc cagcagtgca aaattctccg gcttgaagta acgccgcaca
     1441 cctcgcgccc ggacagaatc gacttccgac ccctggcgat attttccggt tagaaagccg
     1501 cctgccagcg ggctgtacgg tatgacgccc agctggtatg tctggcatac atccgccagc
     1561 tcccgctcca cttcagcccg gttcagcagg tgataatgag gctgcagcga aacaaatctt
     1621 gccagcccat cgcgctgcga ggtccataat gcctgcatca ggcgccaggc aggaaaattt
     1681 gacgctccga tatagcgcac tttgccctgg tggaccaggt catcaaaggc gcgcagagtc
     1741 tcttcgatcg gcgggtgttc ttcataggca tgcgcctgat acaggtcgat ataatctgtt
     1801 cccagccggc gcagggaatc ctcgaccgcc tgcatgatgt ggacccggga taacccacct
     1861 tcatttggac cattccccat cttgccgcga actttggtcg cgatcaccag cttcgggcgc
     1921 gggatctggc tttgcttgat ccaacgtcca atgatttctt ctgcaacccc accgggattc
     1981 ccctcgaccc accgggaata aacatccgcg gaatcgatga aattgatccc ggcctcataa
     2041 gccaggctga ggatctggtg tgaagttgct tcatctgcac tccagccaaa ttgcatggtc
     2101 cccaggcaaa gctcggaaac ctgcagtcca ctgtgtccta agtagcgata ttccatcgaa
     2161 ttcctcctgc ggatcggtcc gtttatcata gatgacctgt caatgttctg ataccctggg
     2221 cttattttcc ccgaaaaatt tgcacccgat cttcatttag ccgcggattg cccggtagat
     2281 caacaccaat ccaaatccag ccagaatgaa gatcccgact accatcatcc cgcgcaccag
     2341 ttttggcgct ttctggcggg caaaatggag cgctaaaaca aggccgagca ttgcg
//
LOCUS       DATMXK010000059         3999 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_19683, whole genome shotgun sequence.
ACCESSION   DATMXK010000059 DATMXK010000000
VERSION     DATMXK010000059.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3999)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3999)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3999
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19683"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(173..1048)
                     /locus_tag="VN363_01115"
     CDS             complement(173..1048)
                     /locus_tag="VN363_01115"
                     /inference="COORDINATES: protein motif:HMM:TIGR00167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWQ83131.1"
                     /translation="MSIVNAKEIMVKAAEGKYAVGAFNITNLIQMEAVVQAAVDKKAP
                     LIIQTSVKPSKFLGRDVLVAIYRSLAEAAPIPICLHLDHCTDVEYCKRCADAGYTNIM
                     IDASKQPYDENIRQTKEVVDYCHAIGGISVEGELGTVGGVEDQIIVAEDEAQLANPQQ
                     AVEFVERTGLDIFAPAIGTAHGVYKTKNPKIDFERLGAINQIMNGNGIQTPLVVHGGT
                     GLPEDYIVKLLANGGAKFNVSTELKRTLIDATYEYITAKRDEYDPGKIDEAVNKAIRA
                     VVGHWIDMLGSAGKA"
     gene            complement(1139..2134)
                     /gene="dhaK"
                     /locus_tag="VN363_01120"
     CDS             complement(1139..2134)
                     /gene="dhaK"
                     /locus_tag="VN363_01120"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HWQ83132.1"
                     /translation="MKRFVNDPKDFVPEMLKGIALANPKTLKYIPEYNLIMRTDIPMA
                     DKVSIMQGSGSGHEPAHVMTVGKGMLDAACPGNVFAAPPADFVYNTTKLVASPKGVLH
                     LLNNYTGDTMSFEMGREMAEAEGIEVRTVTINDDVAVKDSLYTIGRRGVAGNFFVMKA
                     VGAAAEQGKDLAELESIGNHVVSVVRTMGMALTGCTPPAKGSPIFTLAEDEMEMGIGI
                     HGEPGRKRVKLAPANAIVDELFEAVSTDLPYKAGDEVALMINGMGGTPISELYILYAH
                     AHELCEKLGLKIKRNYVGEYCTSLEMAGFSLTLVKLDPELEGYLAAPAEIAIRVF"
     gene            complement(2180..2803)
                     /gene="dhaL"
                     /locus_tag="VN363_01125"
     CDS             complement(2180..2803)
                     /gene="dhaL"
                     /locus_tag="VN363_01125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HWQ83133.1"
                     /translation="MSKDVEFVVRTIADTVLVNEAYFSELDGVVGDGDFGYSIARGFE
                     KVLGEWDNIDRSLPGNFLKKISSLFISAVGGVSGTIWGTAFLRAGMSLGTKETIARED
                     IIPMLRSAIEGIKKRGNSDLGDKTLLDALIPAVDALEKAINEGKMGSQALEEAALVAR
                     ETAEATRPMLAKRGRAAYTGERSIGTLDAGAVAMAVLFEAVSKAWSE"
     gene            complement(3051..3773)
                     /locus_tag="VN363_01130"
     CDS             complement(3051..3773)
                     /locus_tag="VN363_01130"
                     /inference="COORDINATES: protein motif:HMM:NF019322.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWQ83134.1"
                     /translation="MLNKSEGIPLYIQIRRTLRDEISNQVLVPGQKLPSEDELAARFG
                     VSRMTVRQGISDLIDEGLLYRRHGVGTFVALPHLDRDHTRLTSYLESARQEGLATAVQ
                     VLTGEVIPAKRKVALELKLNEGELVIRVKTLRYISGVPVTLHDAYIPYKLFPQLLQED
                     LKNKHLWDIFESYGFRVKRAVQRLEAREADEVSAALLEVDEGSPILYKERTVYLDNGT
                     PVEFTYCYNRGDRYRLTVVLDR"
BASE COUNT          781 a         1130 c         1119 g          969 t
ORIGIN      
        1 gctcgtgata gtaatctacg tcccaatcga cagggtagcc gaattccttg aaggtctcat
       61 tgaaagagac ccggtgcccg tcccgctcag tgtcgatgat gacgccatcc tggtcgaaaa
      121 ataaggcttt gatttgagcc atctggacgc ctccttcgaa ttcccctctg gttcaggctt
      181 tgccggcgct gcccagcata tcaatccagt gcccgaccac cgcccggatt gccttgttca
      241 cggcctcatc gatcttgccc ggatcgtact cgtcgcgttt ggcggtgatg tactcgtagg
      301 tggcatcgat cagggtccgc ttcagctcgg tagagacgtt gaatttcgct cccccgtttg
      361 ccagcagctt gacgatgtaa tcttcgggca gaccggtacc gccgtgaact accagcgggg
      421 tctgaatgcc gttgccattc atgatctggt tgatcgcccc caggcgctcg aaatcgatct
      481 ttggattctt ggtcttatac acgccgtggg cggtgccgat tgccggggcg aagatatcca
      541 gcccggtgcg ctcgacgaat tccactgcct gctgggggtt tgccagctgg gcttcatcct
      601 ccgccacgat gatctggtcc tcaaccccgc caaccgtgcc gagctcgcct tcaaccgaga
      661 tgcccccgat ggcatggcag taatcgacga cctcttttgt ctggcggata ttctcatcgt
      721 aaggctgctt ggaggcgtcg atcatgatat tcgtatagcc ggcatcggcg cagcgcttgc
      781 agtattccac gtctgtgcag tgatccaggt gcaggcagat cgggatcggg gcagcttctg
      841 ccaggctgcg gtagatggct accagcacat cccggccaag gaatttggag ggctttaccg
      901 atgtctggat gatcaggggg gcttttttat ctacagctgc ctggacgaca gcttccatct
      961 ggatcaggtt ggtaatattg aaggcaccga cggcatattt gccttcggca gctttgacca
     1021 tgatctcttt ggcattgact atagacattc accacctccg ggtaggtaaa tggtcattct
     1081 ggaggctgga atgccagcct ccagaatcta ttttgaacga caacaggctg agcctgaatt
     1141 agaagacccg gatggcgatt tccgccgggg ccgccaggta gccttccagc tctggatcaa
     1201 gcttgaccag ggtgagcgag aagccggcca tctcaagcga ggtgcagtat tcaccgacat
     1261 agttgcgttt gatcttcaag cccagtttct cgcacagctc atgagcatgg gcatacagaa
     1321 tgtacagctc ggagatcggt gtgccgccca tgccgttgat catcagggcg acttcatcgc
     1381 cggctttgta gggcaggtcc gtggagacgg cttcgaagag ctcgtccacg atggcattgg
     1441 cgggcgccag cttgacgcgc ttgcggcccg gctcgccgtg gatgccgatg cccatttcca
     1501 tctcatcctc agccagggtg aagatcgggc tgcctttggc gggcggcgtg cagccggtga
     1561 gcgccatacc catggtgcgc actaccgata ccacatggtt gccgatcgat tcgagctcag
     1621 ccaggtcctt gccctgctcg gcggcggcgc cgacagcttt catcacgaag aagtttccgg
     1681 ctaccccgcg gcggccgatg gtgtacagcg aatccttgac cgcgacatcg tcgttgatgg
     1741 taactgtgcg tacttcaatg ccctcggctt cagccatttc gcggcccatc tcgaaggaca
     1801 tcgtatcgcc ggtgtagtta ttcagcaggt gcagcacacc tttcggcgat gcgaccagct
     1861 tggtggtgtt gtacacgaag tcggctggcg gggcagcgaa gacatttccc gggcaggctg
     1921 catccagcat gcccttgccg accgtcatca catgcgccgg ttcgtgccct gagccagagc
     1981 cttgcatgat cgagacttta tcggccatag ggatatcggt ccgcataatc aggttgtatt
     2041 cggggatgta cttgagagtc ttggggtttg ccagggcaat ccctttcagc atttcgggta
     2101 caaaatcttt cggatcatta acgaaccttt tcatcaggaa ctcctttcag cctcatccaa
     2161 atagtgggtt tccaaacgtt cattcactcc aggccttgct gactgcctcg aataagacag
     2221 ccatggcaac tgcaccggca tccaatgttc caatgctgcg ttcgcctgta taggcggccc
     2281 ggccgcgttt ggcgagcatg gggcgggtag cttcagccgt ctcccgggcc accagcgcag
     2341 cttcttccag agcctgactg cccatcttac cttcattaat tgctttttcc agcgcgtcca
     2401 ccgccgggat cagtgcatcc agcagggttt tatcgcccag atcgctgttg ccgcgctttt
     2461 tgatcccctc gatggcggag cggagcatgg ggatgatatc ttccctggcg atcgtctctt
     2521 tcgtccccag ggacatcccg gcgcgcagga aggctgtccc ccagatggtg ccggagaccc
     2581 cgccaactgc gctgatgaaa agggaagata tttttttgag gaaatttccg ggcaggctgc
     2641 ggtcgatatt gtcccattcc cccagtactt tctcgaaacc gcgggcaatc gaataaccaa
     2701 aatccccatc cccgacaacg ccgtccagct cgctgaaata agcttcattt acaaggacgg
     2761 tgtcggcaat ggttcgcaca acaaactcaa catctttgct cattggcaat ctcccttttt
     2821 gagttgtgga gcgcccactc tggcagtctt cggcacgggt ccctcccgag cgcctgcctt
     2881 gagggtgttt cgatcggatt cttgctcacg tacaggcatt aggatcggat agtggacgcc
     2941 tccttgttgt ttcagcagta atagtccggt caaggcaagt tcgatggtag agccgtgcgc
     3001 acacgctctg cgcttgcgaa cgccacctgg gcatgccctg agtttattcc ttaacgatcc
     3061 agcactacgg tcagccggta gcggtcgccg cggttatagc agtaagtgaa ttccaccggc
     3121 gtaccgttat ctaaatacac ggtgcgctct ttgtacagga taggcgagcc ctcatccact
     3181 tccagcagcg cggcgctcac ttcgtcagct tcgcgcgctt ccaggcgctg cacggcgcgc
     3241 ttcacccgga agccgtagga ttcgaagata tcccacagat gtttgttttt tagatcttct
     3301 tgcagcagtt ggggaaaaag cttgtaggga atgtaggcgt catggagagt tacgggaacg
     3361 cctgagatgt agcgcagggt ttttacccgg atgaccagct cgccctcgtt gagcttcagc
     3421 tcaagggcga ctttgcgctt ggcggggata acttctccgg tgagcacctg gacagcagtt
     3481 gccaggcctt cctgacgggc agattccaga tagcttgtca ggcgagtgtg gtcgcgatcc
     3541 aggtggggca gggcgacgaa ggtacccacg ccgtgccgcc ggtaaagcag accttcatca
     3601 atcaggtcgg aaatcccctg gcggacggtc atacggctga caccgaagcg ggctgccagc
     3661 tcgtcctcgg agggtagctt ttgcccgggc accagcactt gattggatat ttcatcccgc
     3721 agggttctgc ggatctggat gtacagcggg atgccttccg atttgttcaa catggttatg
     3781 ttgctcatcc gggtaattcc gcgtccgtcc gtcgactcca gtctgatcgt ccagtcaggg
     3841 ttgtatatac gattaaggga agatgtgtag acgtctacat attagatgat tctgagagac
     3901 aagtcaagta ggtgaatctt aaaaaattcg gctgttctgc ccatgtttgt aacttttaag
     3961 ctcccgttta ttgactttat ccaggatttt gctaagatg
//
LOCUS       DATMXK010000060         8671 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_122866, whole genome shotgun sequence.
ACCESSION   DATMXK010000060 DATMXK010000000
VERSION     DATMXK010000060.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8671)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8671)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8671
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122866"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            644..1705
                     /gene="pheS"
                     /locus_tag="VN363_01135"
     CDS             644..1705
                     /gene="pheS"
                     /locus_tag="VN363_01135"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560878.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit alpha"
                     /protein_id="HWQ83135.1"
                     /translation="MLENLDQIEKAALVSLEDVQDEESLQQWKIVYLGRSAPLMQVFD
                     QMGKLSKEERPLVGRKANEVKRALEAALAERTQALGQQSLERSLQSEKLDVTLPGRPQ
                     TLGRLHPATRTLNEITRVFGDMGFQVYRSPEVETDEVNFQLLNFAVGHPARDMQDTYF
                     VESSRERGDNPVLMRTHTSPGQIHAMREYSAENPDNPPPVRIILPGMCFRYEQVTVRH
                     EMQFNQVEVLAVGKNITFGDLKGTISEFARRMFGQQVRTRFRASYFPFTEPSAEMDVE
                     CFVCGGTGCGVCKGSGWLEIMGCGMVHPVVLRNGGYDPARYSGFAAGMGPERVTMLRH
                     RIEDIRSFWANDMRFLEQF"
     gene            1717..4308
                     /gene="pheT"
                     /locus_tag="VN363_01140"
     CDS             1717..4308
                     /gene="pheT"
                     /locus_tag="VN363_01140"
                     /EC_number="6.1.1.20"
                     /inference="COORDINATES: protein motif:HMM:TIGR00472.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009328 - phenylalanine-tRNA ligase
                     complex [Evidence IEA];
                     GO_function: GO:0004826 - phenylalanine-tRNA ligase
                     activity [Evidence IEA];
                     GO_process: GO:0006432 - phenylalanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phenylalanine--tRNA ligase subunit beta"
                     /protein_id="HWQ83136.1"
                     /translation="MKLPLSWLKDFVDIDLAPEELASQLTMAGLEVEEIRYVGLPLPG
                     GANELHSSASRPEAKISGLEWDAAKIVTAAVSEVMPHPNADRLVLCRLFDGLGEQIVL
                     TGAPNLYPFKGIGPLDKPLKVAYAREGARLYDGHQAGQVLTTLKRAKIRGVDSYSMIC
                     SEKELGISDEHEGVIILDEDAPVGVPLVDYMGDAVLEISILPNTARAANVIGVAREIA
                     AITGKPLKVPADFYAQHASGAPIQARIEILEPELNPRFVLGLVRDVKIGPSAFPIQRR
                     LRLAGMRPINNIVDATNYAMLEVGQPLHAFDYDVLVQRAGGGPPTILTRTARPGERLT
                     TLDGVERSLDASTVLVCDTAGALSIAGIMGGAETEVSEATRNVLLEGAAWNFINIRKT
                     AQTQNLPSEASYRFSRGVHPALAERGVQRGLELMRAWAGGEVAEGLVDNYPLPPEDPQ
                     VEVTPDDVQRWLGISLKADQIAALLIRLEFSVVVQGDTVLVTTPDHRLDIGAGITGVA
                     DLMEEVARVYGYDNLPETRLADELPPQRGNPSLEREDRIRELLVSLGLQEVISYRLTS
                     VEREARLFTRIEGGSELENFGCPIQPYVRLMNPISSDRGVLRQSLLSSVMEVLDRNAR
                     LRQRLALFEIGPVFQADGDAGLPREPQRIALLLAGPRSVAGWQPADSGSMDFYDLKGV
                     LETWLGSLHIQDLRYAPVVHPSYHPGKCARILAGEQLIGVFGELHPLVRANFDLPVSY
                     AEAPIVTAELDLDALQPLIPTLYAAEAVPEYPPALEDLAVIVSETMPAEQVESVIREA
                     GGKMLVGLRLFDVYRGEQIGTGMKSLAYGLIYQSPDHTLSEKEVQQVRARIIRRLEQD
                     LGAHLRS"
     gene            complement(4425..6293)
                     /locus_tag="VN363_01145"
     CDS             complement(4425..6293)
                     /locus_tag="VN363_01145"
                     /EC_number="1.5.1.20"
                     /EC_number="2.1.1.10"
                     /inference="COORDINATES: protein motif:HMM:NF006396.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional homocysteine
                     S-methyltransferase/methylenetetrahydrofolate reductase"
                     /protein_id="HWQ83137.1"
                     /translation="MSGSPFLQFIDQSPLPLLSDGAMGTLLNGRGISFERCFDELNRT
                     RPAVVAEVHREYIEAGAQVVQTNTFGANRYKLAAYGLEDQVAEINRAGVELARRVVMA
                     SYKSVFVAGDIGPLGVRLAPFGRVQPEQARLAFSEQIRALAEAGADLLIIETFSDLHE
                     IREAVTSARQVCDLPVVASLTFTRDDRTLLGDSPQKVARLLTETGADLIGVNCSGGPA
                     QIFRILRLMRQEVPQARFSVMPNAGWPEHIGGRIMYPSNEDYFGEYTLSFCQAGASLI
                     GGCCGTTPQHIAAMRAALDTHPEGCRSEVSVSQSHEAEEASPEDNATSPLTQKLAHGE
                     FIVSVEMDPPRGLSAHKLLAGASLLAEAGADVINVADSPMARMRMSPWAVCELIQQRV
                     GADTVLHFPTRGRNLLRVQGDLLAAHALGIRNVFVVMGDPTSIGDYPEAMDNYDLVPS
                     GLIKLIKQGFNAGLDHAGADIGQPTSFFVGCALNLCPPDPDREIKNLRRKLAAGADFI
                     LTQPVFDPAPAQALLARYTELHGPLTTPVFVGILPLFGARHAAFLHNEVPGITIPEQL
                     RERIRQAGEHAPQEGVRIALELTDQMRQFAHGIYLMPAFQRYDLAADIVEGTKAGS"
     gene            6365..>8671
                     /locus_tag="VN363_01150"
     CDS             6365..>8671
                     /locus_tag="VN363_01150"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SMC family ATPase"
                     /protein_id="HWQ83138.1"
                     /translation="MTGVYKSLNLADNEEYMIPLHLSIAGFLSYRDPVEIDLSAIELA
                     CIAGANGAGKSSLLDAITWVLFGQARKRDDSIINSQSDMASVGLIFSYEGNCYRVLRS
                     KPRDKTTVLEFHILQSGQPLPGDGAQTVWKPLTERSLRETEQIIQNTLRLDYETFVNA
                     SFFLQGKADQFTQQRPGDRKRILSSILGLDIWEQYRLQAVERRKHVEAEISIQSGRLD
                     EISAELAEEANRRAQLKAMETNLEQASKERMLQEQALHSIREVAAALENQTRLVDNLQ
                     QQLAVAERRRMDLAGRLESRRRERDEFAELNARSEAIQAAYQRLQEAREELERLNDVA
                     SRFREQEKRREGPRDEIKAAQARLLQEAQSLEGQLRQIDVLMAEAVRLESQQKEAAQA
                     VAMAEEQVHQREALELALQGSQQLRAESQAEKSRLRAEMTPIKERIEKLAETEGAQCP
                     VCGKDLAFHERDKLIEELTTQGKTLGEQFRLLTTTLTGLDEEMKNQEARLTALRQIES
                     QLHAQHQRLAQVNSRLEVVQGQIQTWQDSGAPRLREIRQALDEESYAAEARQRLAEVD
                     EELWGIGYDAAAHDAIRREVGEGRKAEEDSRALERARAALAPLEREIAELETEAAVQD
                     EQLDRQREETTSAAAALAEAQSKAPDLRAAERVMYDLQERENQVRLEVGAARQKVLVL
                     DDLKQRRKTLEEKRKELRLSESRFRQLEKSFGKDGVPALLIEQSLPEIEGRANAVLER
                     LSSGTMTVRFITQAQYRDKKRDDLRETLD"
BASE COUNT         1888 a         2350 c         2833 g         1600 t
ORIGIN      
        1 attgtcggta aaaaatgacc tttctcgatg gagatgatcg actggcgcgc aactccgacc
       61 atttctgcca gctgctcctg ggtcaatccg gaattggcgc gggtctcacg gacacagttc
      121 ttcacgattg gggatggcat atgtaaagtg tacattacat tccgtaaaat gtcaagtgta
      181 ctttacatta ttttttggct tgagttccag tcgcttgaca ttgcaggttt ttcgcggata
      241 attaggcata catagaataa cggctgtgat ggaggaaagt aagccaggca ggttcaaatg
      301 aacttgctga aaccgacagg gaggcctggg gcatagactg agaccccggc ccggggcaaa
      361 ctggctgaag ttcccttctg agccacccag gggaacgatc ttcgtgcgcc acgcctggcg
      421 ctgcaggtcc agtagtttgg gtcgcaatcc cagcgttaac cgggagacca atcaccgctc
      481 aggcggtcgt tgggtcgagt gggctgtatc cacagccaag cagggtggta ccgcgggaaa
      541 attcccgtcc ctagataggg gcgggttttt gtttactacc gcaaagccca cgccgctgga
      601 acacaggcgg caaatgcttt gatttatcga ttatggagga agtatgctcg agaacctgga
      661 tcagattgaa aaagcagcgc tggtctccct ggaagacgtc caggatgagg agagcctgca
      721 gcagtggaag attgtatatc tggggcgcag tgcgcctttg atgcaggtat ttgaccagat
      781 gggcaagctc tcgaaggaag agcgcccgct ggttggccga aaggcaaacg aggtaaagcg
      841 cgccctggaa gccgccctgg cggagaggac ccaggcgctg ggccagcaaa gcctggaacg
      901 cagcctgcaa tcggagaagc tggatgtgac cctgcctggc agaccgcaaa ccctgggcag
      961 gctgcacccg gcaacgcgaa cgttgaatga gatcacgcgg gtgtttgggg atatgggctt
     1021 tcaggtttac cgctcacctg aagtggaaac cgacgaggtc aacttccagc tgcttaactt
     1081 cgcggtggga cacccggcgc gggatatgca ggatacttat tttgtggaaa gcagccgcga
     1141 gcgcggtgat aatccggtat tgatgcgcac gcatacttcc cctggccaaa tccatgccat
     1201 gcgcgagtat tcggcagaga atccggacaa cccgcctccg gtgcggatta tcctgccggg
     1261 gatgtgcttc cgctacgagc aggtcaccgt ccggcacgag atgcagttca accaggtgga
     1321 agtgctggcg gtaggcaaaa acatcacttt tggcgacctg aaagggacga tcagtgaatt
     1381 cgcccggcgc atgttcgggc agcaggtgcg cacccgcttc cgcgcctcgt acttcccttt
     1441 tactgagccg agcgccgaaa tggacgtgga atgttttgtg tgcggcggca cgggctgcgg
     1501 cgtctgcaaa ggttcgggct ggttggagat tatgggctgt ggaatggtgc acccggtggt
     1561 tttacgcaac ggcgggtacg acccggcgcg ttacagtggt ttcgcggcag gcatgggccc
     1621 ggagcgggtc acgatgctgc gccaccggat tgaagatatt cgctcgttct gggcgaatga
     1681 tatgcggttc ctggaacaat tctgagggat ggtgcaatga aattaccctt atcctggctt
     1741 aaagattttg ttgatatcga tctcgctcct gaagagctgg cgagccagct cacgatggcg
     1801 gggctggagg tagaggaaat ccgttatgtg ggcctgccgc tgccgggtgg agcgaatgaa
     1861 cttcattcgt ctgccagccg ccccgaggcg aaaatcagcg ggctggagtg ggacgcggca
     1921 aaaatcgtga ctgcagctgt ctcggaagtg atgccccacc ccaacgctga ccggctggtg
     1981 ctgtgccggc tgttcgatgg cctgggcgag cagattgtgc tcaccggcgc gccaaatctc
     2041 tacccgttta agggtatcgg tcccctggat aagcccttga aggtggctta tgcccgggaa
     2101 ggggcgcgcc tgtatgacgg gcaccaggct ggacaggttc tgaccacgct caagcgggcc
     2161 aagatccgcg gggtggattc ctattcgatg atctgctccg agaaagagct ggggatttcc
     2221 gacgagcacg agggcgtgat catcctggat gaggatgctc cggtgggtgt gccgttggtg
     2281 gattacatgg gcgatgcggt attggagata tcgatcctgc cgaatacagc ccgggcggca
     2341 aacgtcatcg gggtagcgcg tgaaatcgcc gccatcaccg gtaagccgct caaagtcccc
     2401 gcggacttct atgcgcagca cgcaagcggt gccccaatcc aggccaggat tgagatcctc
     2461 gagccggagc tgaacccacg tttcgtgctg ggattggtgc gggacgtcaa aattggtccc
     2521 agcgcatttc ctatccagcg ccgcctgcgc ctggcgggca tgcgcccgat caacaatatt
     2581 gtggacgcca ccaactacgc catgctggaa gtgggccagc cgttacatgc ttttgattac
     2641 gatgtgctgg tccagcgagc cggtggagga ccccccacca tcctcacccg tacggcgcgt
     2701 cctggtgagc ggctgaccac gctggacggc gtggagcgca gcctggatgc ctctacggtg
     2761 ctggtgtgtg atacagccgg ggcactctcg attgcaggaa ttatgggcgg ggcggaaacg
     2821 gaagtgagtg aagctacccg caatgtgctc ctggagggcg cagcctggaa cttcatcaat
     2881 attcgcaaga ccgcccagac gcaaaacctg ccttctgagg ccagctaccg attctcgcgc
     2941 ggcgttcatc cggcgctggc ggaacgcggt gtgcagcgcg gcctggagct gatgcgggcc
     3001 tgggccggag gcgaggtggc ggaaggcctg gtggataatt acccgctgcc gcccgaagat
     3061 cctcaggtgg aagtcacccc agacgatgtc cagcgctggc tgggtatatc tctgaaagct
     3121 gatcaaattg ctgccctgct catacgcctg gaattttcag tggtcgtgca gggtgatacg
     3181 gtgctggtca ccacccccga tcaccggctg gatatcggcg ccgggattac cggtgtagca
     3241 gacctgatgg aggaagtggc ccgggtctat ggatacgata atctccctga gactcgcctg
     3301 gcggacgagc tgccgcccca gcgcggcaat ccgtcgttgg aacgtgagga tcgcatccgc
     3361 gagctgctgg tgagcctggg cctgcaggaa gtgatcagct accgcctcac ctcggtcgag
     3421 cgggaggcgc ggctcttcac ccgtattgag ggaggcagtg agctcgagaa tttcggctgc
     3481 ccgatccaac cgtatgtgcg cctgatgaat ccaatttcca gcgaccgggg tgtgctgcgc
     3541 cagagcctgc tttccagcgt catggaggtg ctggatcgca atgcccgcct gcgccaacgc
     3601 ctggccctgt tcgaaatcgg gccggtcttc caggcggatg gggacgccgg gctgccgcgt
     3661 gagccgcagc gaatcgctct gctcctggcc gggccgcggt cggtggccgg ctggcagccg
     3721 gcagattcag gcagcatgga tttctatgac ctgaaaggcg tcctggaaac gtggctgggg
     3781 agtctgcata ttcaggattt acgctatgca cccgtggtgc atcccagcta ccatccgggc
     3841 aagtgcgccc ggatcctggc aggcgagcag cttatcggcg tatttggcga gctgcaccca
     3901 ctggtgcggg ctaacttcga cctgccagtg agctatgccg aagcccccat cgtgaccgcc
     3961 gagctggacc tggatgcctt acaaccattg attcccaccc tgtatgctgc cgaggcagtg
     4021 cctgaatacc caccagcact ggaagatctg gcggtaatcg tctctgaaac gatgccggcg
     4081 gaacaggtgg aaagtgtgat tcgcgaggca ggaggcaaaa tgctcgtggg gctgcgcctg
     4141 ttcgatgtgt atcgcggtga gcagatcggg accgggatga agagcctcgc atacggcctg
     4201 atttaccagt cgcccgacca cactttgagc gaaaaagaag ttcaacaagt gcgcgccagg
     4261 atcatccgcc gcttagagca agatctgggg gcgcacctgc ggagctgatc agaacactcc
     4321 tcaggttaag accacactta cgttgatcga cggaggggaa taaggaaata taaaacgggc
     4381 tttgagatta tttcccaaag cccgtttcgc gttaaattgg cagcttagct gccagccttg
     4441 gtcccctcga caatatcggc tgccagatcg tagcgctgaa aagctggcat cagataaatt
     4501 ccgtgcgcaa actggcgcat ctgatcggtc agctcgaggg cgatgcgcac accttcctgt
     4561 ggggcgtgct cgcctgcctg gcgaatccgc tcgcggagct gctcgggaat ggtgatcccg
     4621 ggcacttcgt tgtgcaggaa ggcagcatgg cgagcaccga agaggggaag gataccgaca
     4681 aaaacggggg tagtgagcgg accgtgcagc tcagtgtagc gagcgaggag tgcctgggca
     4741 ggggccggat cgaagacagg ctgggtgagg ataaaatctg ccccggcagc cagcttgcgg
     4801 cgtagattct tgatctcgcg gtcagggtcc ggcgggcaca aattcaaggc gcagccgacg
     4861 aagaacgaag taggctgacc gatatctgcg ccggcatgat ccaggccggc attgaagcct
     4921 tgtttgatga gcttgatcaa accggaaggg accaggtcgt aattatccat tgcttccggg
     4981 tagtcgccga tggaggtggg gtcgcccata acgacgaaga cattgcggat acccagggca
     5041 tgggctgcca gaaggtcccc ctgaacgcgc agcaaattgc ggccgcgggt ggggaaatgc
     5101 aggaccgtat cggcgcctac ccgctgctgg atcagctcgc agactgccca gggactcatg
     5161 cgcatgcgcg ccatcgggct gtcggcaaca ttgatcacat ccgcgccggc ttccgccagc
     5221 aggctggcgc ctgccaggag cttgtgcgct gaaagaccgc ggggtggatc catctctacc
     5281 gagacaatga actccccgtg ggcaagcttt tgggtcaggg gcgaagtggc gttgtcctca
     5341 ggagaggctt cctcggcctc atggctttgt gagacactga cttcggagcg acagccttcg
     5401 ggatgggtat ccagggcggc gcgcatggcg gcgatgtgct ggggggtggt gccgcagcag
     5461 ccgccgatca ggctggcgcc tgcctggcag aaggagagcg tatattcccc gaagtaatct
     5521 tcgttggaag ggtacatgat ccgcccgccg atatgctcgg gccagccggc attgggcatc
     5581 accgaaaaac gcgcctgggg gacttcctgg cgcatcaggc gcaggatgcg gaagatctgc
     5641 gccgggcccc ccgagcagtt tacgccgatc agatcagcgc cagtttcggt gagcaggcgg
     5701 gctaccttct ggggtgaatc gcccaacagg gtgcggtcat cgcgggtaaa ggtcagtgag
     5761 gctactaccg gcaggtcgca cacctggcga gcggaggtta ccgcctcgcg aatttcatgc
     5821 aaatcactga aagtctcgat gatcaaaagg tcagcgcctg cttcagccag ggcgcggatc
     5881 tgctcgctaa aagccagtct ggcttgctcg ggctggacac gcccgaacgg cgccaggcgg
     5941 acacccaggg gaccgatatc cccggcgacg aagacggatt tgtaggacgc catcaccacc
     6001 cgccgggcaa gctctacacc ggcccgattg atctcggcga cctggtcttc caggccataa
     6061 gcagccagtt tgtagcggtt cgctccgaaa gtattggtct ggaccacctg ggcgccggct
     6121 tcgatatatt cgcggtggac ctcggcaacg acggcgggac gggtacggtt gagctcatca
     6181 aaacaacgct caaagctgat gccgcgcccg ttgagcaggg tacccatggc accatcgctc
     6241 aggagcggga ggggagattg atcaataaat tgcaggaagg gagaaccgga cataatggac
     6301 caattttact acgtctaggg gtggaacgag ctggcattct gtactcagga cagggtttgt
     6361 tataatgaca ggagtttaca aatcactaaa tctggctgac aacgaagaat acatgatccc
     6421 attacatctg tctattgctg gctttttaag ttaccgtgat cccgtggaga tcgatctcag
     6481 cgcgattgaa ctggcctgta ttgccggtgc gaacggcgcg ggaaaatcat ccctgctgga
     6541 tgccatcacc tgggtgctgt tcggccaggc gcgcaagcgt gacgattcga tcatcaattc
     6601 tcaatcggat atggcttcgg tcggcctgat attttcatat gagggaaact gttaccgggt
     6661 actgcgcagc aagccgcgcg ataaaacgac ggtgctggaa ttccatatct tacagagcgg
     6721 gcagccatta cccggggatg gggcgcaaac ggtgtggaaa ccactcaccg agcgttctct
     6781 gcgggagacc gagcagatca tccagaatac cttacgcctg gattatgaaa cattcgtcaa
     6841 tgcctcattt tttctgcaag gcaaagctga ccagttcacc cagcaaagac cgggagaccg
     6901 caagcgtatc ttgagcagca tcctgggact ggatatctgg gagcagtacc ggctgcaggc
     6961 tgtcgagcgg cgcaaacacg tagaagccga gatatctatt cagagtgggc ggctggatga
     7021 aatcagcgcc gagctggcgg aagaggccaa ccggcgcgcc cagctgaaag caatggaaac
     7081 gaacttagag caggcgtcca aggagcggat gctgcaggag caggctctgc acagcattcg
     7141 cgaagtcgcg gcggcgctgg agaatcagac ccggctggtg gacaatttgc agcagcagct
     7201 ggctgtggca gagcggcggc ggatggatct ggcaggacgt ttggagagcc gccggcgaga
     7261 gcgagacgag ttcgcagagc tcaacgcccg gtcggaggca atccaggctg cctaccagcg
     7321 cctgcaagaa gcacgggaag agctggaacg cctgaacgac gtggcaagcc ggtttcgcga
     7381 acaggagaaa cgccgggagg gcccgcggga tgagatcaag gcggcgcaag cgcgcttgct
     7441 gcaggaagca caatcactgg aaggacagct gcgccagatt gatgtcctga tggcggaggc
     7501 ggttcgcctg gagagccagc agaaggaagc cgctcaggct gttgccatgg cggaggagca
     7561 ggttcaccag cgcgaggcgc tggagctggc attacaggga agccagcagc tgcgggcgga
     7621 atctcaagca gaaaaaagcc gtttgagagc cgagatgacc ccgatcaagg aaagaattga
     7681 aaagctcgcg gagacggagg gggcgcagtg cccggtgtgt gggaaggatc tggcgtttca
     7741 tgagcgcgat aagctgatcg aagagctaac gacgcagggc aagacattgg gcgagcagtt
     7801 ccgcctgcta acaaccacac tgaccggcct ggatgaagaa atgaagaacc aggaggcgcg
     7861 cctgacagcc ctgcgccaga tagaaagcca gctgcatgcc caacaccaac gcctggcaca
     7921 ggtcaacagc cgcctggaag tcgtccaggg gcagatccag acctggcagg atagcggggc
     7981 gccgcgcttg cgcgagatcc gccaggcttt ggacgaagaa agctacgccg cggaggcgcg
     8041 ccagcggctg gctgaggtgg atgaagagct ctgggggata ggctacgatg cagccgcaca
     8101 cgatgcgatc cggcgcgaag tgggagaagg gcgcaaggca gaagaagaca gccgcgccct
     8161 ggaacgcgcc cgggctgccc tggctccact ggagcgcgag atcgccgagc tggaaacgga
     8221 agcagccgtg caggacgagc agcttgatcg ccagcgagaa gaaacgacca gcgcggcagc
     8281 cgctctggca gaagcacaga gcaaagcacc tgacttgaga gccgccgagc gggtaatgta
     8341 tgacctgcaa gagcgggaaa accaggtgcg cctggaagtg ggcgctgccc gccagaaggt
     8401 gctggtgttg gacgacctca aacagcgccg gaaaacactg gaggaaaagc gcaaagagct
     8461 gcgactcagt gagagccgct tccgccagct ggagaagtct tttggcaagg atggggttcc
     8521 cgcactgctg atcgagcagt ccttaccgga gatcgaaggg cgggctaacg ccgtcctgga
     8581 acgattgagc agcgggacga tgacggttcg tttcatcacc caggctcaat accgggataa
     8641 aaaacgggac gacctgcgcg agaccctgga t
//
LOCUS       DATMXK010000061         2927 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_7697, whole genome shotgun sequence.
ACCESSION   DATMXK010000061 DATMXK010000000
VERSION     DATMXK010000061.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2927)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2927)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2927
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7697"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..535)
                     /locus_tag="VN363_01155"
     CDS             complement(<1..535)
                     /locus_tag="VN363_01155"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ83139.1"
                     /translation="MDETALILEAKRGDLDSFNRLVLAYQDMVYNQAFRMMGELDAAE
                     DATQEAFISAYKSIHIFRGGSFKGWLLRIVTNACYDELRRRKRRPTTPLEPVDEDDEE
                     IESPHWIADPSETPEESTERQELSLAIQNCLNELPEEFKTVVVLVDLQAIDYTEAAAV
                     MQVPLGTIKSRLARARLR"
     gene            complement(537..920)
                     /locus_tag="VN363_01160"
     CDS             complement(537..920)
                     /locus_tag="VN363_01160"
                     /inference="COORDINATES: protein motif:HMM:NF022700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3054 domain-containing protein"
                     /protein_id="HWQ83140.1"
                     /translation="MARSNLILIFGDTLVILIIAMIGFASHGTLDSSGGRLLATLVPF
                     LVAWFASALPLQALDPQATRGWGIIRPAWAVILAAPLGAFLRGIWLRIPIQATFVLVM
                     AAFLLLGLLLWRIFYNYLIFPRTHT"
     gene            1078..1572
                     /locus_tag="VN363_01165"
     CDS             1078..1572
                     /locus_tag="VN363_01165"
                     /inference="COORDINATES: protein motif:HMM:NF016221.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016531 - copper chaperone activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="copper chaperone PCu(A)C"
                     /protein_id="HWQ83141.1"
                     /translation="MKHVELIPILGLILLMLAGCKTPPAPATLTAEDAWIRAIGSTES
                     TEHTMEMPDPTPGTTIELNTAAFVKIVNSSAEADRLIRVESDLALTVEIHLSEMKDEV
                     MTMRPVTGIDILAGETLNFEPGGYHIMLINLRHGIKAGEKYPLTLIFEKAGSLTIDAE
                     VLAP"
     gene            complement(1582..>2927)
                     /locus_tag="VN363_01170"
     CDS             complement(1582..>2927)
                     /locus_tag="VN363_01170"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ83142.1"
                     /translation="AFPPEKIQTRLNTTLELAGLTGLRSRRLDTLSGGERQRVALAAV
                     LALSPTILVLDEPTSQLDPEAAECILQILVRLNKDQGLTILLAEHRLERILPFINSMI
                     YMDGDNGSVLHGEPRQVLAKVKLVSPVIALAKQLGWQPLPLTVEEARPFAENVRFSEP
                     AGQAAGDQHPPVYEFALSIENLHVYYGPKPAITDINLGVQHGEILALMGPNGAGKTTL
                     LRTLVGLVKPDRGTIRLLGEPTQSMDTAELSLSIGYLPQDPNSLLFAESVLDELLFTL
                     RSRAQRSNDPLRLKPAEWEANAAEVANRLLSQLDMADLAAAYPRDLSVGQRQRVALGA
                     IMVARPKVLLLDEPTRGLDAAAKEQLAEILRNWRDEGHAIILVTHDVELAARIADRIA
                     LLEDGNLTAVGQPAEVLSASPVFTPQILQLFPDSGCLTPEDLLRRLNPVGTQPAR"
BASE COUNT          734 a          837 c          739 g          617 t
ORIGIN      
        1 gtctcaacct ggcccgcgcc aggcgactct tgatcgttcc gagcggtacc tgcataaccg
       61 ctgccgcctc ggtataatca attgcctgca aatcgaccag gaccaccaca gttttgaatt
      121 cctctggcag ctcattcagg cagttttgga tcgccagact caattcctgt ctttcggtgc
      181 tttcctctgg cgtctcgctg ggatcggcaa tccagtgggg agattcaatt tcttcgtcat
      241 cctcatccac aggctcgagg ggagtcgttg gccggcgttt tcgacggcgc agctcatcat
      301 agcaagcatt cgtcacaatc ctcaatagcc agcccttgaa ggagccgcct cgaaaaatat
      361 ggatactctt ataagccgag ataaacgcct cctgggtcgc atcttcggcc gcgtccaatt
      421 cccccatcat ccgaaacgcc tggttgtaaa ccatatcctg ataggctaaa accaggcgat
      481 tgaaagaatc taaatcacca cgtttggctt caagaattaa agctgtttca tccatgttaa
      541 gtgtgggtgc gagggaaaat taagtagttg tagaatatcc gccagaggag caatcccaac
      601 aaaaggaaag ctgccataac cagcacaaaa gtagcctgga taggtatccg aagccagatg
      661 ccacgcagaa aggctcccaa tggagccgcc aagatgactg cccaggccgg acgaattatc
      721 ccccaacccc gggttgcctg gggatccaat gcctggagcg gtaaggcact tgcaaaccag
      781 gcaacgagaa aaggaaccag ggttgccagg agccggccac cagatgagtc cagcgtccca
      841 tggctggcga agccaatcat ggcaatgatc aaaatgacca gtgtatcccc gaaaatgaga
      901 attaaattgc ttcgcgccat gctcacgcct acatccaaat ataatcctat cgcgaaggca
      961 gaaaaggggc aagggtgtat aaatctcaaa ccccgtcaat aatagatatc gacctaacag
     1021 agatgttata ctggcttccg taattgcagc gaatacattt caagagagat gcaatcgatg
     1081 aaacatgtcg aactgattcc aatcctggga ttaattctcc ttatgctggc aggatgcaaa
     1141 actcccccag ccccggcgac tctcacagct gaagatgcct ggatccgggc gataggcagt
     1201 acagaatcaa cagagcatac gatggaaatg ccggatccca cacctggaac aacaattgag
     1261 ctaaacacgg cagctttcgt aaaaatcgtg aattcaagcg ccgaagctga tcggctgatc
     1321 cgggtcgaga gtgaccttgc cctaacggtc gaaattcatc tgtcggaaat gaaggatgag
     1381 gtcatgacca tgcgcccggt cacggggatc gatatcctgg caggcgaaac gctcaatttc
     1441 gaaccgggtg gttaccacat tatgttgatc aacctccgcc acggcatcaa agccggcgag
     1501 aaatatcccc ttacgcttat ttttgagaaa gccggttcgc taacaatcga cgctgaggtg
     1561 ctggctcctt agccgccaaa ctcaccgcgc tggctgcgtt ccaacaggat tcagacgcct
     1621 gagcaaatcc tcaggagtca ggcagccaga atcgggaaat aattgcagta tctggggagt
     1681 gaatactggc gaggccgaga gcacttctgc gggctgccca acggcggtca gattcccatc
     1741 ctcgagaagc gcgatccggt ctgctatacg cgcagccagc tccacatcat gggtaaccaa
     1801 aataatcgcg tgcccctcat cccgccagtt tcgtaagatc tctgccagct gctctttcgc
     1861 cgcagcgtcc agcccccggg tgggctcatc cagcaaaagc accttcggtc tcgcaaccat
     1921 gatcgctccc aacgccacgc gctggcgctg gccaacactc aagtcgcggg gatacgccgc
     1981 agctaaatcc gccatgtcca gttgggacag cagccggttt gccacctcgg cagcgttcgc
     2041 ctcccactct gcaggcttca acctgagcgg atcgttagat ctttgcgccc ggctgcgtaa
     2101 agtaaacagc agctcatcca ggacactctc tgcgaacaac aacgaattag gatcctgagg
     2161 taaataacca attgacaggg agagttcggc tgtatccatg gactgggtag gctctcccaa
     2221 caaccggatc gttccccggt ctggcttcac cagacctacc aaagtcctaa gcaaagtcgt
     2281 tttgccggcc ccattgggtc ccatcaatgc caatatttca ccatgttgga ctcccagatt
     2341 gatgtctgtt atcgcaggtt tgggaccata atagacgtgt aaattttcta tactcaaagc
     2401 aaactcataa actggagggt gttgatctcc tgctgcctgt ccagccggct ccgaaaaccg
     2461 cacattctct gcaaagggtc gggcttcctc cacagttaac ggtaacggct gccaacccag
     2521 ctgcttggcc agggcgatca ccggggagac cagtttgact tttgccagca cctggcgcgg
     2581 ctctccgtgt agcacagatc cgttatcacc gtccatataa atcatgctgt tgataaaagg
     2641 caggatgcgt tccagacgat gttccgccag gaggatcgtc aatccctggt ccttgttcag
     2701 gcgaaccaga atttgtagaa tgcattcggc tgcctcagga tccaattggg aagtcggctc
     2761 atccaaaacg aggatcgtgg gggataatgc cagaaccgcc gccagggcca cccgctggcg
     2821 ctccccgcct gaaagtgtat ccaggcggcg gctgcgcagc ccggtcagcc ccgccaattc
     2881 cagggtcgtg ttcaggcgcg tctggatttt ctcgggcggg aaggcaa
//
LOCUS       DATMXK010000062         3322 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_99235, whole genome shotgun sequence.
ACCESSION   DATMXK010000062 DATMXK010000000
VERSION     DATMXK010000062.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3322)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3322)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3322
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99235"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..459
                     /locus_tag="VN363_01175"
     CDS             22..459
                     /locus_tag="VN363_01175"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWQ83143.1"
                     /translation="MKIQEPEIQISELFKALSQPARIEILLAIGTSEACVCHLEAQLT
                     MRQAYISQQLMALRQAGILETRRDGRYIYYRLADPAVLELISQAAQVLQIPAEKTQRA
                     DLEQAMAACPCPHCEPQPAARLIELPIEPRRTSAVSRSRPDRF"
     gene            593..2077
                     /locus_tag="VN363_01180"
     CDS             593..2077
                     /locus_tag="VN363_01180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="permease"
                     /protein_id="HWQ83144.1"
                     /translation="MDIPAFLLRLFQGGMGNLAAYLAAHVLLCLLPAFYIAGAMTALI
                     PKDTVTRFLGRNTPRFISYPAAALAGSVLAVCSCTIVPLFAGIYKKGAGIGPAITFLF
                     FAPAANILALVYTGGVIGPDLAFARLFLSLAFGIGIGLIMALIFRKDDQVHDKNTDAM
                     FAAQNAKGMPRASLAFLLVLVALLLAGTLKLPFLTEAAAQTTLPVAGAAEFQAGLDRL
                     VPYDAAQGEEGVSVQGVILIGMLVLVGITSWKGFENIHEGANRWTGIALGLTAITLSV
                     ASLGMRASPDGLIISLPGKFFAVLLALGVLFWLGSSRISADERRDFLWESWKFVKQIF
                     PLLVVGVFAVGMIRVIIKPEWIETLAGSNTLLGNMAGVLFGVFMYFPTLVEVPIARMF
                     LSLGMHRGPLLAYLMADPELSLQSILIISAIIGRKKTWTYVSWVALFSTLAGLIYGAW
                     IDGTSLWILALYLGSFLAVLGTILFLVNKRVAKKTSLPQSAATD"
     gene            2124..2369
                     /locus_tag="VN363_01185"
     CDS             2124..2369
                     /locus_tag="VN363_01185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560509.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWQ83145.1"
                     /translation="MLTIKVLGPGCANCKRLEQIATKVVTELGIQAQVVKVTDYNDIM
                     SYDILSTPGLVINDKVVSYGRIPSPAEVTGWVTSALA"
     gene            2394..2972
                     /locus_tag="VN363_01190"
     CDS             2394..2972
                     /locus_tag="VN363_01190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83146.1"
                     /translation="MAAASSGTATTPRGTIMLNYAYWNRWVFGFLMMGLTLSACANVT
                     AQVALVEPVIEETGYEKFDLKNCEQPLDDIHTPVTEYFNVKQEVTIADQATAAATGAS
                     YTLSAAEKKAMADQVEAAYQKDYAAAQEVLEEQEFVVPKDRAANFSITWQAKIYQASL
                     DFEAKGETHTVEYQYTLTVPVVGEREIAICGG"
     gene            complement(3114..>3322)
                     /locus_tag="VN363_01195"
     CDS             complement(3114..>3322)
                     /locus_tag="VN363_01195"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="catalyzes the formation of
                     4-imidazolone-5-propanoate from urocanate during histidine
                     metabolism; Derived by automated computational analysis
                     using gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWQ83147.1"
                     /translation="IHHGGGVGIGYSIHAGQVTVADGTEAAARRLQRVLTSDPAMGVV
                     RHADAGYPEAIEFAKKTGVKIPMM"
BASE COUNT          687 a          947 c         1005 g          683 t
ORIGIN      
        1 aatatattca ggtatgcgaa tatgaaaata caagaacccg aaatccaaat ctcagaatta
       61 ttcaaagccc tcagccagcc agcacggatc gagatcctgc tggcgatcgg cacgagcgaa
      121 gcctgtgtgt gccacctgga agcgcagctg accatgcgcc aggcgtatat ctcccagcag
      181 ctcatggcct tgcgccaggc gggcatcctg gagacgcggc gggatgggcg gtacatctat
      241 taccgcctgg cggatccagc ggtgctcgag ctgatatccc aggcggctca agtactgcag
      301 atcccggctg aaaagaccca gcgggcagac ctggaacaag ccatggcggc ctgcccgtgc
      361 ccgcactgtg aaccccagcc ggccgcacgc ctgatcgaac ttccgatcga gccgcgaagg
      421 acctccgctg tgtccaggag cagaccggac aggttttaag gcaacctgaa tcgaccggca
      481 tccggcgggg aacaataatc cgccggggct ggcagcaaac ggaaacctgt acggtcatgt
      541 atgaagaagg attatcgcgg aagattgaaa aaacacctga gggaagatat cgatggatat
      601 tccagcattt ttgttgaggt tatttcaagg cgggatgggc aacctggctg cctacctggc
      661 agcgcatgtc ttactctgcc tgctgccggc attctatatc gccggggcga tgaccgccct
      721 gatccccaag gataccgtca cccgcttttt ggggcgcaac acgcccaggt tcatttccta
      781 cccggcagct gccctggccg gctcggtgct ggcggtgtgc tcgtgcacca tcgtgccgct
      841 gttcgccggg atctataaaa aaggcgccgg gatcggcccc gccatcacct tcctgttctt
      901 tgccccggca gccaatatcc tggcgctggt gtacaccggc ggggtgatcg gccccgacct
      961 ggcttttgcc cgactgttcc tctccctggc ctttgggatc gggattggcc tgatcatggc
     1021 cctgatcttc cgcaaggacg accaggtgca cgataaaaac acggatgcca tgttcgccgc
     1081 tcaaaacgct aaagggatgc cccgagcctc cctggctttc ctgctggtgc tggtggcgct
     1141 gctgctggcg ggcacgctca agctcccatt tctgacggag gcagcggctc aaaccaccct
     1201 gcctgtggca ggcgccgcag aatttcaggc cgggctggac cggctggtgc cgtatgacgc
     1261 cgcccaaggt gaagaaggcg tttccgtgca gggcgtgatc ctgatcggca tgctggttct
     1321 ggttggaatt acttcctgga agggttttga gaatatccac gaaggcgcca accgctggac
     1381 cgggattgcc ctgggactga ccgccatcac gctgagcgtc gcctccctgg ggatgcgggc
     1441 ttctccagac gggttgatca tttctttacc ggggaaattc ttcgctgtgc tgctggcgct
     1501 gggagtattg ttctggctgg ggagcagccg catctcggcg gatgagcggc gcgatttcct
     1561 gtgggagtcg tggaagttcg tcaagcagat cttccctttg ctggtggtgg gcgtgttcgc
     1621 agtgggcatg atccgggtga tcatcaagcc ggaatggatc gagaccctgg cgggcagcaa
     1681 taccctgctg gggaatatgg caggggtcct gttcggagtg ttcatgtact tccccaccct
     1741 ggtggaagtg ccgatcgcca ggatgttcct gagcctgggc atgcaccgcg gcccgctgct
     1801 ggcgtacctg atggcggacc cggagctgag cctgcagagc atcctgatca tctcggcgat
     1861 catcgggcgc aagaagacct ggacgtatgt gagctgggtg gcgctgttca gcacgctggc
     1921 cgggttgatc tacggcgcct ggatcgatgg gaccagcctg tggatcctgg cattgtacct
     1981 gggttccttc ctggcggtgt tagggaccat cctgttcctg gtcaacaagc gggtggctaa
     2041 gaaaaccagc ctgcctcaat cagcagcgac agattaattt ctgtgaaatt gcacaaaaca
     2101 aatcaatcac tatggaggtt tcgatgttaa caatcaaagt tttaggtccg ggctgcgcca
     2161 actgcaaacg cctggagcaa attgccacca aagtcgttac cgagctggga atccaggctc
     2221 aggtagtcaa agtcactgat tacaacgaca tcatgtccta cgacatcctg tccacaccgg
     2281 gattggtgat caatgataag gtcgtgtctt acgggcgaat cccttcgccg gcagaagtaa
     2341 ccggctgggt gacgagcgcg ctggcgtaag tcacaggttt agtggtagca ggcgtggcag
     2401 ctgcttcctc cgggacagct accacgccgc gcggaaccat catgttgaat tatgcatact
     2461 ggaatcggtg ggtgttcggt tttttgatga tgggtttgac cctgagcgcc tgcgccaatg
     2521 tcactgccca ggtcgccctg gtcgagccgg tgattgaaga aacaggctac gaaaaattcg
     2581 acctgaagaa ctgtgagcag ccgctggacg atatccatac gcccgtgacg gaatacttta
     2641 acgtcaagca ggaggtcacc atcgcggacc aggcgactgc agcagccacc ggcgccagct
     2701 atacgctatc ggcagctgaa aaaaaagcaa tggctgatca agttgaagcc gcctatcaaa
     2761 aggattatgc cgcggctcag gaagtgctgg aagagcagga atttgtggtc cccaaggacc
     2821 gggcggccaa cttcagcatt acctggcaag ctaagatata ccaggcctcc ctggattttg
     2881 aagccaaggg cgaaacccac accgttgagt accagtatac gctgaccgtc cccgtggtgg
     2941 gggagcggga gatcgcaatc tgcggcggtt gagtacatta accgctcccc ggaggggtag
     3001 cactttgggt agcacgttgg gtagctgagt tgggaaagaa accaccatcg tgagggcggg
     3061 aatccacgag aggtggtttt tttacccgag gaacagaccg tacaggtttg caactacatc
     3121 atcgggatct tcaccccggt ctttttggca aactcgatcg cctccgggta gccggcatcc
     3181 gcatgccgca ccacgcccat tgccggatcg ctggtgagga cgcgctgcaa gcgccgcgcc
     3241 gcggcttccg tcccatccgc caccgtcacc tgcccggcgt ggatgctgta gccgatgccc
     3301 acccccccgc cgtggtggat gc
//
LOCUS       DATMXK010000063         4660 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_122770, whole genome shotgun sequence.
ACCESSION   DATMXK010000063 DATMXK010000000
VERSION     DATMXK010000063.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4660)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4660)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4660
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122770"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1447)
                     /locus_tag="VN363_01200"
     CDS             complement(<1..1447)
                     /locus_tag="VN363_01200"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWQ83148.1"
                     /translation="MAEPLIITPEKRLQRRLLVLATLFMLIYAAALTISPVVRAREWT
                     GWPADLRWTHWLGVGAWLVFAWLLEGWINRRLPDHDPYLLPITSLLLGWGLLTIYRLW
                     PEAGLRQSAWVLFGGALAGLGIARLTPDLSSLRRYKYVWLTGGLLLTALTFWLGTNPL
                     GYGPRMWLGAFGIYLQPSEPLKLLLIIYLAAYLSEPGTALPASQHPERSELLRLLAPT
                     LVMTGLALGLLVVQRDLGTASIFLFLYTIIVYLSTARRIVPLSGALVITAAGVAGMLL
                     FDLVRLRIDAWLNPWLDPSGRSYQIVQSLLAVANGGVFGRGPGLGNPGVVPVPHSDFI
                     YASIVEEAGLMGGLAMLALLAMLVGRGLQIAMRAADPFRRLLAGGLTAYLGGQSILII
                     GGNLRLLPLTGVTLPFVSYGGSSLMVSLAAVMLLVMISSQPATRLVPQKSNQPYLHVG
                     GLLFAGLIGASLTLGWWGVVRGPDLLLRTDNP"
     gene            complement(1461..2327)
                     /gene="mtnP"
                     /locus_tag="VN363_01205"
     CDS             complement(1461..2327)
                     /gene="mtnP"
                     /locus_tag="VN363_01205"
                     /EC_number="2.4.2.28"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559561.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0017061 - S-methyl-5-thioadenosine
                     phosphorylase activity [Evidence IEA];
                     GO_process: GO:0006168 - adenine salvage [Evidence IEA];
                     GO_process: GO:0019509 - L-methionine salvage from
                     methylthioadenosine [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S-methyl-5'-thioadenosine phosphorylase"
                     /protein_id="HWQ83149.1"
                     /translation="MQSKPTLAVIGGTGLHSMPGLENIQTLDVDTPFGKPSAPITVGI
                     ISGIPVAFLSRHGIGHHLLPGEVPYRANIYALKALGVERIVSVSACGSLREDFAPGSI
                     VIPNQIFDLTRGRKSSFFGEGLVAHISVADPFCPDLSAQLFAAAKMRTPNVHKGGGYV
                     TIEGPRFSTRAESNTYRSWGMSLIGMTASPEIFLAREAEICYATMAHVTDYDVWHVSE
                     EPVNVEMVMDILNRNLSLAQDIILTLASDLAEARDCQCSSALENTLITNPQVIPAETR
                     SRLDLLVGKYLP"
     gene            complement(2371..2805)
                     /locus_tag="VN363_01210"
     CDS             complement(2371..2805)
                     /locus_tag="VN363_01210"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWQ83150.1"
                     /translation="MNISAPVLLALRVVLALVLYGFLALALNLTWKNLRATSQRLAVP
                     QFPRLALLPADTEAEPGYYNTQIVLVGRDPASDLHLDDPTISAHHARISFHHGQWWLE
                     DLHSRNGTLLNQEAVSEPVVITGGDSLRCGQVELRIVIEQAH"
     gene            complement(2802..3602)
                     /locus_tag="VN363_01215"
     CDS             complement(2802..3602)
                     /locus_tag="VN363_01215"
                     /inference="COORDINATES: protein motif:HMM:NF023818.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FhaA domain-containing protein"
                     /protein_id="HWQ83151.1"
                     /translation="MNYNNPVNKPRKTNRLAQIETRLQSLIEGSAARFFTRSTPEQNL
                     AQRLANEVLRGLHQGADGILVAPNLYTLFVSPEHLDELQANPGLFDQLSQTLEEAAAQ
                     AGVSFSSPPVLRVYPETSLRGHEITIHSQDSLADLAQTSELSQDELLHGGEFPPNAFL
                     IVDGVRTFPLRRSVINIGRRSDNHLVVDDARVSRVHAQLRAVRRRYLLFDLDSTGGTF
                     LNGERIRQATLSPGDVITLAGVPMVYGQDPSSETQDIGFVPSGPEVFR"
     gene            3668..4117
                     /locus_tag="VN363_01220"
     CDS             3668..4117
                     /locus_tag="VN363_01220"
                     /inference="COORDINATES: protein motif:HMM:NF014058.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="archease"
                     /protein_id="HWQ83152.1"
                     /translation="MDTFENGFRGQAGFREHPHTADWELEVWAADLPGLLEQAARGML
                     HLAGARALDTPRTTLSLRLTAADVESLLVSFLGELLFKMEQDGLVFSGFDLQVESLPE
                     DAGLALNANLLAQPLLSLDKEIKAVTYHHLHVQAAPEGLRTRIVFDV"
     gene            4138..>4660
                     /locus_tag="VN363_01225"
     CDS             4138..>4660
                     /locus_tag="VN363_01225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008452 - RNA ligase activity [Evidence
                     IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RtcB family protein"
                     /protein_id="HWQ83153.1"
                     /translation="MVALKDLKKISDYEWEIPQSYRGDMRVPVRLFATRRQLEAIASD
                     QSLEQAVNAATLPGLVGQVTIMPDMHQGYGFPIGAVAATRLPDGVVSPGAVGYDINCG
                     VRLLGSQLEFRQVEPYLDELATTLNAYCPSGVGEAGSVKLSENDLEQVCRDGARWALK
                     NGYASPADLRRTEA"
BASE COUNT          935 a         1378 c         1477 g          870 t
ORIGIN      
        1 aggggttgtc ggtgcgcaaa agcaggtcgg ggccgcgcac cacaccccac cagcccagcg
       61 tcagggaggc gcctatcagg ccggcgaaaa gcagccctcc tacatgcaga taaggctgat
      121 tggatttctg cggaaccagg cgcgtggcag gctggctgct gatcatcacc aggagcatta
      181 cagctgccag ggagaccatt aacgaagagc ctccgtagga aacgaatggc agggtcacgc
      241 cggtcagtgg cagcaagcgc aggttgccgc cgatgatcaa tatgctctgc ccgccgaggt
      301 aagcggtcag cccgccggct agcaggcgcc ggaatggatc ggcagcccgc atggcgatct
      361 gcaggccgcg ccccaccagc atcgccagca gcgccagcat tgccaggccc cccatcaggc
      421 cagcttcctc tacgatagag gcataaataa aatcggagtg cggcacaggc accacccctg
      481 ggtttcccag accaggcccg cgcccgaaaa cgccgccatt ggcgactgcc agtaaggact
      541 gcacaatctg ataggagcga ccgctggggt ccaaccacgg attcagccag gcgtcgatgc
      601 gcaggcggac caggtcaaag agcagcatgc ctgccacgcc agccgcggta attaccagcg
      661 ctccgctgag cggtacgatc cgccgggcgg tgctcaggta gacgataatg gtatataaga
      721 acaggaagat cgaagccgtg cccagatcac gctggacaac cagcaggccc agcgccagcc
      781 cggtcatcac cagggtgggc gccagcagtc ttagcagctc gctgcgttcc gggtgctggc
      841 tggcgggcag ggcagtgcct ggctcgctca ggtatgccgc caggtagata atcagcagta
      901 attttagcgg ctcggagggc tgcagataaa tcccaaatgc gcccagccac atgcgcggtc
      961 catagccgag cgggttggtt cccaaccaga atgtcagggc ggtcagcagc agcccgcctg
     1021 tcagccagac gtatttgtag cggcgcaggc tgctcaggtc gggggtcagc ctggcgatgc
     1081 ccagaccagc cagtgcccct ccgaagagca cccacgcgct ctggcgcagg ccagcctctg
     1141 gccataaacg gtagattgtc agcagccccc agcccagcag caggctggtg atgggcagca
     1201 ggtaaggatc gtggtccggc aggcgccggt ttatccagcc ttccagaagc caggcaaaca
     1261 ccagccaggc gccaaccccg agccagtgcg tccagcgcag gtcggcaggc cagccagtcc
     1321 attcacgggc ccggacaacc ggggagattg tgagcgcggc ggcgtagatc agcataaaca
     1381 gcgtcgccag gaccagcagg cgacgctgca aacgtttttc gggggtgatt atgagcggtt
     1441 cagccaagct ggtttgaggc tcagggcagg tatttcccca ccagcagatc caggcggctg
     1501 cgtgtctcag cagggattac ctgggggttg gtgatcaggg tattctccag ggcgctgctg
     1561 cattggcagt cgcgtgcctc cgccaggtca cttgccaggg tgaggatgat gtcctgggcc
     1621 aggctcaggt tgcggttgag gatatccatc accatttcca cgttgaccgg ttcttcgctg
     1681 acatgccaga catcgtaatc ggttacgtga gccatggtgg cgtaacagat ctcggcttcg
     1741 cgagccagga atatttcggg tgaagctgtc atcccgatca gggacatacc ccacgatcgg
     1801 taggtgttcg attcggcgcg cgtggagaac cgtgggcctt cgatggtcac ataccccccg
     1861 cctttatgga cgttcggagt gcgcatttta gctgctgcga ataactgcgc cgacaaatcc
     1921 gggcagaagg gatcggcgac actgatatgc gctaccagtc cttcaccgaa gaaggagctt
     1981 ttgcgacccc tggtcagatc gaagatctgg ttggggatca cgatactgcc aggagcgaaa
     2041 tcctcgcgca aagagccgca ggcactgacg ctgacaatcc gctccacgcc cagagctttg
     2101 agggcataga tattggcccg gtagggaacc tcacccggca gcaggtggtg cccgatccca
     2161 tgccgcgaaa gaaatgcaac cggaatgccc gagatgatac ctactgtgat cggggcgcta
     2221 ggcttgccga atggagtatc gacatccagg gtctggatat tttccagccc gggcatggaa
     2281 tgcaggccgg tgccgccgat gactgccagt gttggtttgg attgcatgaa ggttcctccg
     2341 agagcagagt ttaccggcct gtttgccgga tcagtgcgct tgttcgatga cgatccgcag
     2401 ctcgacttgc ccgcagcgca ggctgtcccc gccagtgatc accaccggct cgctgactgc
     2461 ctcctggttg agcagagtgc cgttgcgcga gtgcaggtct tccagccacc actgtccgtg
     2521 atggaagctg atcctggcgt ggtgggctga gatcgtcgga tcatccagat gcagatcgct
     2581 ggctggatca cggccaacca gcacgatctg ggtattgtaa tagccgggct cagcctcagt
     2641 gtcggctggc agcagcgcca gacgcgggaa ctggggtaca gccaggcgct ggctggttgc
     2701 gcgcaggttt ttccaggtca gattaagcgc aagcgccaaa aaaccgtaca gcaccagggc
     2761 taaaaccacc cgcaaagcca gtagaaccgg ggcgctgatg ttcaccggaa gacctccggg
     2821 ccggaaggca cgaagccaat atcctgagtt tcactggagg gatcctgccc gtataccatg
     2881 ggcacgcccg ccagggtgat tacgtcgccc ggggaaagcg tggcctggcg gatgcgctca
     2941 ccgttcagga aggtcccccc ggtcgagtcc aggtcgaaaa gcaggtagcg cctgcgcact
     3001 gcccgcagct gggcgtggac acgcgagacc cgcgcatcat ccaccaccag atgattatcc
     3061 gagcgccgcc cgatattgat cacactccgc cgcaggggga aagttcgcac cccatccacg
     3121 atcaagaatg cgttcggggg aaattcaccg ccgtgcagga gctcatcctg gcttaactcg
     3181 ctggtctggg ccaggtccgc caggctgtcc tgggaatgga tggtgatttc atgcccccgc
     3241 agggatgtct ccgggtagac ccgcagaacc ggcgggctgg agaacgaaac cccggcttga
     3301 gcagccgctt cttccagggt ctggctgagc tggtcgaaca gtccgggatt ggcctgcagc
     3361 tcgtccaggt gctctggcga gacgaacagg gtgtataagt ttggcgccac cagtattcca
     3421 tccgctccct ggtgcagccc gcgcaggact tcgttcgcca ggcgctgggc taaattctgc
     3481 tctggtgtgc tgcgagtaaa gaaacgcgcc gcgctgcctt caatcaggct ctgcagccgg
     3541 gtttcaatct gcgccaggcg gttcgttttg cggggcttat tcaccgggtt attataattc
     3601 atgccggggg tgattggcgc ggtgtgctag aatgaagcgg gacgtgaact gcaggaggtt
     3661 tggcccattg gatactttcg aaaatggttt tcgcgggcaa gctggttttc gtgagcaccc
     3721 acatactgcc gactgggagc tggaagtctg ggcggcggat ctgcccggcc tgttggagca
     3781 ggctgcccgg ggtatgcttc acctggcagg cgcccgggcc ctcgataccc cgcgcactac
     3841 cctgagcctg cgcctgactg ctgctgacgt ggagagcctg ctggtcagtt ttttaggtga
     3901 gcttctcttc aaaatggagc aggatggtct cgtctttagc ggcttcgatc tgcaggtgga
     3961 aagccttcca gaagatgccg ggttggcatt aaatgcgaat ttactcgccc agccgctgct
     4021 ctcgcttgat aaagagatta aagcagtcac ttatcaccac ttgcatgtgc aggccgcgcc
     4081 ggagggtctg cgcaccagga ttgtgttcga tgtgtgatcc ccggccagga ggccttaatg
     4141 gttgccctca aagacctgaa aaagatcagt gattatgaat gggagatccc ccagagttac
     4201 cgcggcgata tgcgcgtccc ggtgcgcctg tttgccactc gccgccagct ggaagcgatc
     4261 gcctcggacc aatctctgga gcaggcggtc aatgccgcca cactcccggg cttggtaggg
     4321 caggtgacca tcatgccgga tatgcaccag ggctacggct tcccgattgg ggcggtcgca
     4381 gccacccgcc ttccggatgg ggtcgtctcg ccgggcgcgg tcggctatga tatcaactgt
     4441 ggggtgcgcc tgctgggctc gcagcttgaa tttcgccagg tcgagccgta cctggatgaa
     4501 ttagccacca ccttgaatgc ttattgtccc agcggagtcg gagaagccgg gtccgtaaag
     4561 ctcagcgaga atgaccttga gcaggtctgc cgggacggtg cgcgttgggc gttgaaaaat
     4621 ggttatgcca gccccgccga cctgcgccgc accgaagccg
//
LOCUS       DATMXK010000064         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_44234, whole genome shotgun sequence.
ACCESSION   DATMXK010000064 DATMXK010000000
VERSION     DATMXK010000064.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44234"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..324)
                     /locus_tag="VN363_01230"
     CDS             complement(<1..324)
                     /locus_tag="VN363_01230"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWQ83154.1"
                     /translation="MQSTPPEFPPPDEDAVAAVDIEQWLEMYEKMLKIRLFEEQVNDL
                     YKTAKMPGLAHLYSGEEAIAVGVSENLHLDDTITSTHRGHGHCLAKGATVDRMFAELL
                     GKQAGY"
     gene            complement(347..580)
                     /locus_tag="VN363_01235"
     CDS             complement(347..580)
                     /locus_tag="VN363_01235"
                     /inference="COORDINATES: protein motif:HMM:NF025807.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MoaD/ThiS family protein"
                     /protein_id="HWQ83155.1"
                     /translation="MLEIHLGGHLSFYTPGKRSRFSLPIASPSSLAGIVEQLGVPLAE
                     VTIAAVNGELVDIETAQVQPGDKIELYPPIGGG"
     gene            complement(591..2489)
                     /locus_tag="VN363_01240"
     CDS             complement(591..2489)
                     /locus_tag="VN363_01240"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HWQ83156.1"
                     /translation="MRSGNTGKILRVDLTGGSIKEETLDESLYRSYPGGKALAAYFLL
                     RELPLHTDPLSPENMLIFANGLLTGAPVATATRFTAAARSPLTGAYGESEAGGFWGPE
                     LKMAGYEAILITGKAVKPVYLSIRNEQVELHPADHLWGQEPESVQSQIRQELGDDKVR
                     VLQIGRAGENLVKYASLTHEMRHYNGRNGIGAVMGSKNLKAVAVRGTSQRYIDIAQDG
                     RALAEIGKTLAKRVKEHPQSWDLHEKGTMPLVGGLNAGGMLPTNNFRTGVFAGVGQID
                     WTAYEKELLIGRKSCYACSVRCKPEVEVDDRYQVSQKYGGPEYEAVAGFGSNCGVSDI
                     QAVAKANELCNRYVMDTISTSATIAFAMECFERGLIGLQDTGGLELRFGNAEAMLQAV
                     EQIAIRQGFGDQLAEGSRSLALAIGGEAPDFTIQVKGQELSMHDPRGKVNVGIGFAVS
                     ENGADHLTAVHDTLLQNPGSISFQGAQALGIRSPLPARLLNAEKVEQYFIGENWVSLG
                     KALGLCYFGPAPRSFIQVDEVVKAVRAATGWDITLEELLQAGERATNLARLFNAREGF
                     SRQDDTLPKRLFDPLESGPLEGVAYPKAEFEQALTTLYQLKGWDPNTGLPGRERLESL
                     GLGWVLDN"
     gene            complement(2541..3842)
                     /locus_tag="VN363_01245"
     CDS             complement(2541..3842)
                     /locus_tag="VN363_01245"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWQ83157.1"
                     /translation="MWSNISHAFQWKGELPRFYRFKQSFPRPRVTDIPAAVAQALINI
                     DNRIPDLKQKRVAISAGSRGISEIAAITAALVSCLRDRGAQPFIVPAMGSHGGGTAEG
                     QRGMLAELGMTEVSLGAPIISSLDTVELGHLPNGMPVYLDQHAAEADAIVIVNRVKPH
                     TDFVGDFESGLAKMAAIGLGKLRGADTLHRYGVDGLLHLVPAAARLICQHTPVIFGLA
                     SVENAYHEVAEIAAIPPEGIAGPQERDLLQLAYRLMPRFPFPEIDVLIVDEMGKNISG
                     VGMDPKIIGRVKVHGVPDLADCSIRTIAVLRLTPEAHGNASGIGLADVTTQHLVNQID
                     FEATYLNCITSGITGIQRAFLPMVAPTDRAAIETALRVCGRADLREARVVRIQNTLSL
                     GEIDISSALVQEIPSGVAPSTIFNLSFDSNDNLSPFTSPAH"
BASE COUNT          876 a         1149 c         1104 g          934 t
ORIGIN      
        1 gtagccggcc tgtttgccca gcagctcggc aaacatgcga tccaccgttg cacctttggc
       61 caggcagtgt ccgtgaccac ggtgggtact ggtgatagta tcgtccaggt gtagattttc
      121 ggaaacaccc acggcgatcg cttcttcacc gctatacagg tgggccaggc cgggcatctt
      181 ggctgtcttg tacaggtcat tgacctgttc ttcgaacaac cgaattttga gcattttttc
      241 gtacatttcc aaccattgtt ctatgtcaac ggcggcaact gcatcttcat ctggaggagg
      301 aaattccggt ggagtagatt gcatcttttg tttccttgaa agtgggttaa ccgccaccaa
      361 tgggcgggta aagttcaatt ttatcgcccg gctgcacctg ggcggtttcg atatcgacca
      421 actcgccatt caccgctgca atcgtgacct ccgccagcgg cacgcccaat tgctccacga
      481 taccagccag agaagaaggg ctagcgattg gaaggctgaa gcggctccgt ttgcctggag
      541 tgtaaaagct taagtgccca ccgagatgga tttccaacac gaccgccgcc tcagttatcc
      601 agtacccagc ctaaccccag actctccagt cgttcacggc ccggcagccc ggtatttgga
      661 tcccagcctt tgagctggta aagggtagtt aaagcctgct caaattcggc ttttggataa
      721 gccacacctt ccaacgggcc gctttccaga gggtcaaaca ggcgcttggg gagcgtatca
      781 tcctgccggc tgaatccttc tcgagcgttg aaaaggcgcg ctagattcgt ggcgcgctcg
      841 ccagcctgca gcagctcttc cagggtaata tcccagccgg tagctgctcg cactgctttg
      901 acgacttcgt ccacctggat gaaggagcgc ggagctggcc caaaatagca tagtcccaga
      961 gctttaccca ggctgaccca gttctcgcca atgaaatatt gctccacttt ctcggcgttg
     1021 agcaggcgcg ccggcagggg actgcggata cccagagctt gagcgccttg aaaggaaatc
     1081 gatcccggat tctgcagcaa ggtatcgtgc accgctgtaa ggtggtctgc gccattctct
     1141 gagacggcga aaccaatccc cacattgacc ttgccgcgtg ggtcgtgcat ggaaagctcc
     1201 tggcccttca cctgaatggt gaaatctggg gcttcaccgc cgattgccag agctaagctg
     1261 cgcgagcctt ccgcaagctg gtcgccaaac ccctggcgta tggcaatttg ttcaacagcc
     1321 tgcagcatcg cttcggcatt gccaaaccgc agctccaagc caccagtatc ctgtagcccg
     1381 atcaggccgc gttcgaagca ttccattgcg aatgcgattg tggcggaggt agaaatcgta
     1441 tccattacat agcggttaca gagctcattg gcctttgcta ctgcctggat atcgctcact
     1501 ccacagttcg agccgaaacc ggctacggct tcatactctg gtccgccgta tttctggctg
     1561 acctggtagc ggtcgtctac ttccacttcg ggtttgcagc gcactgagca ggcataacag
     1621 ctcttgcggc ctatcaataa ctctttttcg taagctgtcc aatcaatttg cccaactccg
     1681 gcgaatacac ctgtccggaa attattcgtc ggcagcattc cgccagcatt tagtccgccc
     1741 acaaggggca tggtgccttt ttcatgcaaa tcccagcttt gggggtgctc tttaacccgt
     1801 ttcgcgaggg ttttgccgat ctcagctaag gcgcgcccat cctgggctat gtcgatatag
     1861 cgttgactcg tgccgcgcac tgccacagcc ttcaggtttt tgctgcccat tactgcgccg
     1921 atgccattcc gcccgttata atgacgcatt tcatgggtca ggctagcata tttgacgagg
     1981 ttttccccag cgcgaccgat ctgcagtaca cgcactttat catcacccag ctcctggcgg
     2041 atctggcttt ggacggattc cggctcctgg ccccagagat ggtcagcggg gtgtagctcg
     2101 acctgctcgt tgcgtataga caggtaaact ggtttgactg ccttacctgt gatgagaatg
     2161 gcttcgtagc cagccatctt cagctctgga ccccagaacc caccggcctc ggattcacca
     2221 tacgccccgg tcagcggcga gcgagctgcg gcggtgaacc gtgtggctgt agccacaggt
     2281 gcgccggtca gcaggccatt agcaaagatc aacatatttt cggggcttaa tgggtcggtg
     2341 tgtagtggga gttcgcgcag caagaaataa gctgccagtg cttttccgcc cgggtacgag
     2401 cggtatagcg actcgtcaag agtttcttcc ttaatcgatc caccagtcag gtcaacgcgt
     2461 aatattttac cggtatttcc agatcgcatg tccaaaaccc tttcaaacca gcgccgcgcc
     2521 gtgattctcc gggtgggcag ttaatgcgcc ggggatgtaa atggtgaaag attgtcgttt
     2581 gaatcaaagc tcaggttaaa aatggtggat ggggctacgc cagagggtat ctcctgcacc
     2641 agggcgctgg agatatcgat ctcgcccaat gaaagcgtgt tttggatacg cacgacgcgc
     2701 gcttcccgga ggtcggcacg cccgcatacc cgcagcgcag tctcgatggc agctctgtcc
     2761 gttggagcca ccattggtaa gaaggcgcgc tgaataccgg tgataccgga ggtgatgcag
     2821 ttcaggtagg tggcctcaaa gtcgatctgg ttaaccaggt gctgggtggt cacatcggcc
     2881 aggccgatcc cggatgcgtt gccatgcgcc tcaggggtca ggcgcaatac agcgatagtg
     2941 cggatggagc agtctgccag gtcgggtacg ccatgaactt tcacccgccc aatgatttta
     3001 gggtccatgc cgacgccgct gatatttttg cccatttcat ccacgatcaa cacatcaatc
     3061 tcagggaaag gaaagcgcgg catcaggcgg taggcgagct gcagcaaatc acgttcctga
     3121 ggtccggcaa tgccttcagg cggtatggcg gctatctcgg cgacttcatg ataggcgttc
     3181 tccaccgaag ccaggccaaa gataaccgga gtatgctggc agatcaggcg cgcggcagca
     3241 ggcaccaggt gcagcaggcc atccacgccg taacggtgta aagtatctgc gccgcgcaat
     3301 ttacccagcc cgatagcggc cattttggcc agcccgcttt caaaatcgcc cacaaaatcg
     3361 gtgtgcggct tgacccgatt gacgatcaca atagcatccg cttcagcggc gtgttgatcg
     3421 agatataccg gcattccatt gggcaggtgc cccagttcca cagtatcgag cgaagaaata
     3481 atcggcgccc ccaggctaac ctcggtcata cccaattctg ccagcatacc acgctggcct
     3541 tcggcggtac ccccgccatg gctgcccatt gctggcacaa tgaaaggctg cgccccacga
     3601 tcccgtaagc aggaaaccag ggctgcagtg atggcggcaa tctcgctgat accgcggctg
     3661 ccagcagaga tggcaacacg tttctgcttc aggtcgggga tgcggttatc aatattaatc
     3721 agcgcctggg caacagccgc cgggatgtct gttactcgcg ggcgaggaaa gctttgcttg
     3781 aagcgataaa agcggggaag ttcacccttc cattggaagg catgggatat attagaccac
     3841 ataccgtcat tatacggctg attttgtcaa ctgtcaacaa tcgactaaaa ataagtggat
     3901 ttgttgctca tagtgcagtt gggaagcaga tcctggcgag atctggctct cgtcaaggat
     3961 ctgcagctcg gaatgtcctg gcctgggcgt gcatctattt attaagatcc cgcgcttaag
     4021 taattcataa ttgacaaacg cattttttct gctaggataa tat
//
LOCUS       DATMXK010000065         2387 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_110743, whole genome shotgun sequence.
ACCESSION   DATMXK010000065 DATMXK010000000
VERSION     DATMXK010000065.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2387)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2387)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2387
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110743"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..882)
                     /locus_tag="VN363_01250"
     CDS             complement(<1..882)
                     /locus_tag="VN363_01250"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWQ83158.1"
                     /translation="MSQSDDFHEPMEGEVFYPSAQATENATVQEYESLYHESLQDPES
                     FWAARAEELEWFQKWDQVLDGSKAPFYKWFVGGKTNIVYNALDRHLKTFRKNKLALIW
                     EGEPGDTRTYSYFSLNREVCKFSNVLRSMGVKKGDIVTIYLPRIPEQVIAMLACAKIG
                     APHSVVYGGFSVEALAERIEDAQSKVLITADGGWLRGKIVELKNIADEAISRQPTIES
                     CIVVKRTGQDVYMEPGRDYWYHDLISLPISTNSCETEVMDAEDPLFVLYTSGTTGRPK
                     GVLHTHGGYMVYTYTTLK"
     gene            1091..1831
                     /locus_tag="VN363_01255"
     CDS             1091..1831
                     /locus_tag="VN363_01255"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560495.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWQ83159.1"
                     /translation="MKILGIDIGGTGIKGAPVDTDTGVMMGERFRIPTPEGGKPKAVS
                     EVVGEVARHFEWSGLIGCGFPAAVRGGTVLNAANLSKKWIGSNAAGLFSEQTNCTVHV
                     LNDADAAGLAEMTFGAGRGRKGVVLIITIGTGLGTSLFTDGVLLPNVEMGHIEMGGKD
                     AETQASEAARVRNELNWPEWTSVFNRYLQAMEHLLWPDLIILGGGAIKKQDKFFSQLK
                     VGTEIVPAQFLNEAGIIGAALGAHLRMT"
     gene            1840..>2387
                     /locus_tag="VN363_01260"
     CDS             1840..>2387
                     /locus_tag="VN363_01260"
                     /inference="COORDINATES: protein motif:HMM:NF020084.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST N-terminal domain-containing protein"
                     /protein_id="HWQ83160.1"
                     /translation="MSDQIKFYSALATQPDPADAIDALAAQLQTQGFRTGEDAADLAL
                     VFISAHFTQQAEIIRRALETLVSPRVFLGTTAEGVVGDEHEVESQPSISVIAARLPGV
                     ELKPFALQEIDWSCILLQPEDFRQIVQAPAETRLFILLADPFSTPIDDVLQSFNLAYP
                     GIPVTGGMSSGALRSSGNAHLY"
BASE COUNT          563 a          592 c          652 g          580 t
ORIGIN      
        1 ttttaaggtc gtgtatgtat agaccatata tccgccgtgg gtgtgtaaca cgcccttggg
       61 acgaccggta gtgccagaag tgtagagcac aaagagcggg tcttcggcat ccatcacctc
      121 agtctcgcag gagttggttg aaattggcag gcttatgaga tcatggtacc agtaatcccg
      181 gccgggttcc atatatacat cctgtccggt gcgcttaact acaatacaac tttcaatggt
      241 gggctggcga gagatggctt catcggcgat attcttcagc tcgacgattt tgccacgcag
      301 ccagccgcca tctgcggtga tcaacacttt gctctgtgcg tcttcgatcc gttcggcaag
      361 tgcttcgacg ctaaacccac cgtaaacgac gctgtggggt gcgccgatct tggcgcatgc
      421 cagcatagcg atcacctgct ccgggatgcg gggcaggtag atggtgacaa tatccccctt
      481 tttaaccccc atgctccgca ggacattgga aaatttacag acttcgcgat tgagtgagaa
      541 gtagctgtag gttcgggtgt cgccaggttc accctcccag atcagggcga gcttattttt
      601 gcgaaaagtc ttaaggtggc gatccaatgc gttgtaaaca atattagttt tacccccgac
      661 aaaccattta taaaagggtg ccttgctgcc atccagcacc tgatcccatt tttgaaacca
      721 ttccagctct tcagcacgcg ctgcccaaaa agattcgggg tcttgcagcg attcatgata
      781 gagactttcg tactcctgaa ctgtagcgtt ttcggtggcc tgtgcggatg gataaaatac
      841 ctccccttcc atcggctcgt ggaaatcgtc tgactgggac atagggacct cctgctggtt
      901 accgctgatt atatggatgt aaaggacagc cggtgaaaca tacgctgtga ttgtatattt
      961 tagaaaagag cttgtcaacc caaaatatcg tgtatccgag atataatcag gggcagtcat
     1021 ctgttcacct tgatttagaa tatcggaagc gagcccggca cacccaccat ccagcacagg
     1081 gaggacgtat atgaaaatcc tggggataga tattggcgga accggtataa aaggcgcacc
     1141 tgtcgataca gatactggcg ttatgatggg tgaacgcttt cgaatcccta cgccagaggg
     1201 tgggaaacca aaggcagtca gcgaggttgt tggcgaggtc gcccggcatt ttgagtggag
     1261 tggcttgata ggctgtggtt ttcccgctgc ggtgcgcgga ggtactgtac tgaatgctgc
     1321 caacctctct aaaaaatgga ttggcagcaa cgcagccggg ctcttttcgg aacagaccaa
     1381 ctgcacagtg catgtgctta acgatgcgga cgctgctggc ctggctgaga tgacttttgg
     1441 cgccgggcgc ggccggaaag gcgtcgtgtt aatcatcact atcggaacag gattgggaac
     1501 gagcctgttc acggatgggg ttcttttgcc gaatgtagaa atggggcata tcgaaatggg
     1561 cggcaaggat gccgagacac aggcatccga ggcggctcgc gtgcggaatg agttaaactg
     1621 gcccgagtgg accagcgtgt ttaatcgata tttacaggcg atggagcatt tactctggcc
     1681 agatctgatt atcctgggtg gtggcgcaat caagaaacag gataaattct tttcccagct
     1741 aaaagttggg acggagattg tgccggcaca gtttttgaat gaagccggga ttattggagc
     1801 ggccttaggt gctcatttga ggatgacctg aacacaccca tgtcagacca gatcaaattc
     1861 tattcggctc tcgccaccca gcctgaccct gctgatgcaa tcgatgcatt ggctgcgcag
     1921 cttcaaacgc aagggtttag aacaggagaa gacgcagccg acctggcatt ggtatttatc
     1981 tccgctcatt ttacccaaca ggctgaaatc atccggagag cactggagac cctggtttca
     2041 ccccgcgtct tcctgggcac tacagctgaa ggggtggtag gagatgagca tgaagtcgag
     2101 agccaaccct cgatttctgt catcgctgcc aggctccctg gcgtagaatt aaaacccttt
     2161 gctttacagg agattgactg gtcttgtatt ctcctgcagc cagaggattt caggcagatc
     2221 gtacaggctc cggctgagac gcggctgttt atccttctgg ctgacccatt ttcaactccc
     2281 atcgacgatg tcctccagtc attcaatctg gcctacccgg gcatcccggt aactggtggt
     2341 atgtccagcg gagcactgcg atcaagtgga aacgcacacc tctataa
//
LOCUS       DATMXK010000066         3274 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_44145, whole genome shotgun sequence.
ACCESSION   DATMXK010000066 DATMXK010000000
VERSION     DATMXK010000066.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3274)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3274)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3274
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44145"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(395..3214)
                     /locus_tag="VN363_01265"
     CDS             complement(395..3214)
                     /locus_tag="VN363_01265"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWQ83161.1"
                     /translation="MTKLRIPFTRPQLVDRPRLQVRTAEGLRGPLTLITAPAGFGKTT
                     LVASGVANCGMPVAWLSLDKDDNQAGRFLNYLVTALQEADNTIGSEAAQLITASQQAP
                     PEAVLTSLINDLDTVGGEIALVLDDYQYLTSKAAHAAVAFLLEHRPDSLHLVIATRSD
                     PPLPLARLRARGQTVELRAADLRFTVPEAAQFLNEVMGLSLDAEAVTLLEERTEGWIA
                     GLQMAALSMRDRNDKRGFIEGFSGTHRYILDYLLEEVLASQSPEVQRFLLCTSILERL
                     TAPLCDAILKDEAGDTSIILPPSSVILEYLEQANLFLVPLDDERRWYRYHHLFTDLLR
                     ARLDQIYQGLAPRLHARAAAWLEQEKMMVEAVNHALAAGEQDYAARLVEENTTRLLAQ
                     GELNALMGWVGVLPAELRLARPWLCVHQAYALAFAGQLADVPPLLAQAEAAKGVTATQ
                     DATYVVGKSEETGIPSAATFETRAFKGAVAAIRAMTAVMVGQDGEAISQAQQSSELLP
                     EENLWDRAAAAWALGYALRSLGRLREARAAFEEQIRLGLAMGNIWTLVTGLTDLANVV
                     RAQGQIRQARALFEEALTEASKQGARSLGYIARMEASLASVLYEQNELEAANRLLTEA
                     IAHTRQWPNPNHLAYAFALQARVLLAKGDLQGARSLVGKADQVRRSAALTRLNKRMVE
                     AELIRVWLALQAAGFGLEPGDQLKEQATTLVEEWQDELVSSTENVAAFMDEGAETAAI
                     TLARVLLAAGRTEEALSLLERVTHDAQAAGHTERLINSLILTTIASRRSEAFSDDQII
                     AALPALEEALSLAESGGYVRVFLDEGQSMQVLIAQWLSHAGINPLRSYAVRLLSHFEA
                     EPNAVIAAQDKVVPTGNLIEPLSQRELEVLRLIALGKTNLEIARQLIVSRGTIKAHAA
                     SIYRKLDVTNRTEAVARARQLGILP"
BASE COUNT          689 a          957 c          913 g          715 t
ORIGIN      
        1 tgccatactc tcgactggct tccatcaagc ccttcttacc cgagatgagc tccagcacga
       61 cacgagcttt gaattctgga ctgaacttcc gtcgttctac catagtggac ctctttcctg
      121 aagtgtaccc caccccttag gggtgtgtcc agttttatcg atccactaca aagcagggtc
      181 tcatcccctt ccaggtgcat caactttaag cctgtaaacc aatccgacca gcgttgatcc
      241 agatgacccc tgattttgat ctcatagtaa tccggcatag cagccctcct tgttagcatc
      301 tgacaccagt ttagcgtgcg acgaggatcc ccgcatctgc caaaggggat attcaggggt
      361 tatttgcagg ggttatctac aggatgagaa ggggttaggg aaggataccc agctgccggg
      421 cacgagcgac tgcctcagtc cgattggtga catccagctt gcgatatata ctggccgcgt
      481 gggctttgat ggtgccccgt gacacgatga gctgtcgggc gatctccaga ttggtcttgc
      541 ccagtgcgat cagacggaga acttccaact cacgctgact taacggctca atcaggttcc
      601 cggtggggac gactttatct tgcgcggcaa tcactgcatt tggttcggct tcaaagtgag
      661 aaagcaagcg aactgcgtat gatcgtaacg ggttgatgcc ggcatgcgac agccactgcg
      721 cgatcagcac ctgcatcgat tggccttcgt cgagaaagac ccgaacgtaa ccgccggatt
      781 cggccaggct gagcgcttct tccaatgctg gtagagctgc aatgatctga tcgtctgaga
      841 acgcttcgct acgcctagag gcaatcgtag tgagaatcag ggagttaatc aatctttcgg
      901 tatgtccggc agcctgcgca tcatgggtta cacgctcaag tagggaaagg gcttcctcag
      961 tccgccctgc agcgagcagc acacgagcca gggtgatggc ggcggtttca gcgccctcat
     1021 ccataaacgc agcgacgttt tctgtcgaac tcaccagctc atcttgccat tcttcgacga
     1081 gcgttgtggc ttgctctttt agttgatccc ctggctcgag gccaaaacca gctgcttgaa
     1141 gtgcgagcca aacccggatc agttccgcct ccaccatgcg cttgtttaag cgggtcagcg
     1201 cagcgctcct tcttacttgg tcggcttttc caaccaacga ccgagctccc tggagatcgc
     1261 ccttggcgag cagcactcga gcctggagtg caaaggcata agccagatga ttggggttcg
     1321 gccactggcg ggtgtgggca attgcctcag ttagcagacg gttggcagct tctagctcat
     1381 tctgctcata gagcacactg gccaggctgg cttccatacg agcgatatag cccaggctgc
     1441 gggctccctg tttgctggcc tcggtgagtg cctcttcgaa aagcgcacgc gcctgcctga
     1501 tctgaccctg ggctcgtacg acgttggcca ggtcggtcag tccggtaacc aatgtccaga
     1561 tattgcccat ggccaggccc aaccggatct gctcttcgaa tgcagcgcgc gcctcccgca
     1621 ggcgaccaag gctgcgtaaa gcatagccga gcgcccaggc ggctgcagcc cgatcccaca
     1681 agttctcttc cggcagcagc tcgctggact gctgtgcctg tgagatggct tcaccatcct
     1741 ggcctaccat cacggctgtc atggcccgga tagccgccac agcacccttg aacgcccgag
     1801 tttcaaaggt agctgccgac ggtatgcctg tttcttctga tttgccaacc acataagtcg
     1861 cgtcttgtgt agcagttacc ccctttgcag cctctgcctg ggctagcagc ggcggcacat
     1921 cagccagctg gccggcgaaa gcgagagcat atgcctggtg gacacacagc cagggccgag
     1981 ccaggcgcaa ttccgccgga agcaccccga cccagcccat caacgcgttc agttcacctt
     2041 gtgctaacag gcgggtggta ttctcctcca ccaggcgagc ggcgtagtcc tgttctccgg
     2101 ccgccagggc gtggttgacg gcttccacca tcatcttttc ttgttcaagc caggctgcgg
     2161 cgcgtgcatg cagccgaggt gccagccctt ggtagatctg atccagccgg gcgcgcagca
     2221 gatcggtgaa caggtggtga taacggtacc agcgccgttc atcatccaat ggcaccagga
     2281 acagattcgc ctgttctagg tattcaagta tgacagatga aggtggaaga atgatagaag
     2341 tatctcctgc ttcatccttc aaaatggcgt cgcacagtgg ggcggtcaaa cgctctagaa
     2401 tcgatgtaca caataaaaag cgctgcactt ctggcgactg gctggccaga acctcctcca
     2461 acaggtagtc cagaatgtag cggtgggtgc ctgagaaccc ttcaataaac cctcgtttat
     2521 cgttacgatc gcgcatggag agggctgcca tctgcaagcc agcaatccag ccctctgtcc
     2581 tttcttcgag cagcgtcact gcctcggcat ccaggctgag gcccatgact tcgttcagga
     2641 actgagctgc ctcgggcacc gtaaaccgga gatcggctgc acggagttca accgtctggc
     2701 cgcgtgctcg caagcgggct aaaggaagcg gtgggtcaga acgggtagcg atcacgagat
     2761 ggagtgagtc ggggcgatgt tcgagcaaaa aagcgactgc cgcgtgcgca gccttgctgg
     2821 ttagatactg gtagtcgtct aacaccaagg caatctcccc gccaacggta tccagatcat
     2881 tgataagact ggtcaggacc gcttcaggtg gtgcttgctg cgatgccgtg atcaattgag
     2941 cggcctcact cccgatcgta ttgtcagcct cttgcagtgc agtgaccaga taattcagga
     3001 agcgccccgc ctgattatcg tccttatcca gagacagcca ggctacgggc attccacaat
     3061 tagcgacacc ggaggccact aatgtggtct tgccaaaacc agcgggagcg gtaatcagcg
     3121 tcagcggacc acgcagaccc tccgcggtcc gtacctggag ccgcggtcgg tcgaccagct
     3181 gtggccgggt gaagggtatg cgtagcttgg tcattatgat cgggtcagcc ttttgcatac
     3241 ggtggtctca ggtcaaacat agactcatcc agat
//
LOCUS       DATMXK010000067         2629 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_87384, whole genome shotgun sequence.
ACCESSION   DATMXK010000067 DATMXK010000000
VERSION     DATMXK010000067.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2629)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2629)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2629
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_87384"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1131
                     /locus_tag="VN363_01270"
     CDS             <1..1131
                     /locus_tag="VN363_01270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83162.1"
                     /translation="LEKLAGFGLLLAIREEGILLGGFVIALNYLLMKGRPGRRLQTLV
                     FLGLETAAAGAFLAFMAWGGYTRVDNVYNPLGFLSTFLSNPLLLALSILLLGAVLLGL
                     AWFKKRSGIDATLLLTIYLGAVALTGAQWLRDTARAFLSLKEANAGLWQYVLAAFTNE
                     STALVAYISILGLALGWAFSRGRARRTLTALVAGLAVFFALITAACMPAQVAQWRQNL
                     APARLVWDFAAGHDRYQTKVLLDYATYQAFYNFDQVIVYNRLPVWDTLPEKRYYPQNK
                     AAVAIQIKKGIAYAVIGRESLANVRELAGLAGVPASELAANERYVILQFQPARPAGSS
                     TAGSVGRLAGAAGRVHRRGVGAGGRSDVCGGAERALTANCWQ"
     gene            complement(1696..>2629)
                     /locus_tag="VN363_01275"
     CDS             complement(1696..>2629)
                     /locus_tag="VN363_01275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83163.1"
                     /translation="VVTKGAGLELATFGAYGDLQVLSTRPFAALSPDGTQIAYVEGDD
                     IWLEPVDQSTPPANLTNSPEIIDSNIQWWPARPGWLVSLYQPVEDMGYSAFIAMLRTD
                     GSEFVPLEDEARSMSVPALSPDGVTIAYDLFGQPALLNVDTGEMNDPGLNVPEHPVQA
                     MGFPAWNADGSQVVFKLYDKEGWIGTGAYTLADSSWKLINEYIINGGTEPWAQSAFSP
                     DGRWLALVNQSDPAVGGFRGPALWVLPLEGGEAILIGAGSNPMWSPDSTRLVYTQSTD
                     GAFDTALLQMVLADSWEPVQLPELPGGYYASSWR"
BASE COUNT          495 a          818 c          758 g          558 t
ORIGIN      
        1 ctcgaaaagt tggccggctt tggcctgctg ctcgccatcc gcgaagaagg catcctcctc
       61 ggcgggtttg tgattgctct aaactacctg ctcatgaaag gcaggcccgg tcgccggctg
      121 caaaccctgg tattcctggg cctggaaact gccgccgccg gtgcgtttct ggctttcatg
      181 gcctggggcg gctacacacg ggtggacaac gtctataatc ccctcggttt cctgtcgacg
      241 ttcctgtcga atcccctgct gctggccttg agcatccttc tcctgggcgc ggttctgctg
      301 ggattggcct ggttcaaaaa gcgctcgggg atcgacgcca cgctcctgct caccatttac
      361 ctgggtgcgg tggcgctgac cggcgcccag tggctgcgcg ataccgcccg ggcattcctc
      421 tcgctcaaag aagccaacgc cggcctctgg cagtatgttc tggccgcttt caccaacgaa
      481 agcaccgccc tggttgccta catcagcatt ctgggcctgg ccctggggtg ggctttcagc
      541 cgcggccggg ctcgcaggac cctcaccgcg ctggtcgcgg gcctggcggt tttctttgcc
      601 ctgatcacgg cggcctgcat gcccgcccag gtggcgcagt ggcgccagaa tctggccccc
      661 gcccgcctgg tctgggattt tgccgccggg cacgatcgct accagacgaa agtccttctc
      721 gactatgcca cttaccaggc attttacaat ttcgaccagg tgatcgtcta taaccgcctg
      781 ccggtgtggg acaccctgcc agaaaaacgt tactaccccc agaataaagc cgctgttgcc
      841 atccagatca agaaaggcat cgcatacgcc gtcatcgggc gtgaatccct ggcaaatgtc
      901 cgggagctcg ccggtctggc tggtgttcct gcctccgagc tggctgccaa cgagcgctac
      961 gtcattttac aattccagcc cgcccggcca gccggcagct ccaccgctgg atctgttggc
     1021 aggcttgctg gagcggctgg gcgagttcac cggcgcgggg tgggagcagg aggacgatct
     1081 gacgtttgtg gtggtgcaga gcgagcgctg acagcgaatt gctggcaatg aatttttacc
     1141 ccgaattgaa cgaattggag tggagaggat ctaattcaat aatcgtagac ttgtctctcc
     1201 gaaattcaga aatattcaaa atgtcagtct attggtctcc cctgacataa ttcgttgaat
     1261 tcgttcccag aattcgttgt cagcctgccg tcaacccgtc gagccggtac accagggcgg
     1321 cgatcaacgc aagagtctac agcggatttt tagaacggat ttcacggatt agttcacaat
     1381 ttggacagtt cacgaagcac aagtcagctc acgttcaaat tcaattctta caaacgaatt
     1441 tcgggctatc cttacccaat ccgtacaatc cgtttttcga atccgttgta tgcccgcctc
     1501 ctgcagccca tcgagcatgc aaacaagacg ctcttcatct gcgtacttct gcgcctgcga
     1561 tcttcgcagt caatctgcgg ataaatcttt aatgtctgga atcaatgaat gaacgaacac
     1621 catctggcat tcgttcattc gcttggattt cgttgattgg cgtcaggaca ggctgaagcc
     1681 tatcctacgg gatatttacc gccagctgga ggcgtagtag ccacccggca gctcgggcag
     1741 ctgcaccggc tcccaggaat ccgccaggac catctgcagc agggcggtat caaacgcgcc
     1801 atccgtggac tgggtgtaca ccagccgcgt agaatccggg ctccacatgg gattggaccc
     1861 cgccccgatc aggatcgcct cgccgccttc caaggggaga acccacagcg ccgggccgcg
     1921 gaagccgcct accgccgggt cggactggtt gaccagcgcc agccagcgcc catccgggct
     1981 gaaggccgac tgcgcccagg gctcggtgcc gccgttgatg atgtattcgt tgatcagctt
     2041 ccagctgctg tccgccaggg tgtatgcgcc ggtgccgatc cagccttcct tatcgtacag
     2101 cttgaacacc acctggctgc cgtccgcgtt ccaggccggg aaacccatcg cctggacggg
     2161 gtgctcgggc acgttcagac cggggtcgtt catctcgccc gtatccacgt tgagcagggc
     2221 cggctggccg aaaagatcgt aggcgatggt cacaccatcc ggcgaaaggg ccgggacgga
     2281 catgctgcgg gcttcatctt ccagggggac aaattcactg ccatccgtcc tgagcatggc
     2341 gatgaacgcc gagtaaccca tatcttccac gggctggtac aggctcacca gccagccggg
     2401 gcgagcgggc caccactgga tattgctgtc gatgatctcg ggcgaattgg tcaggttggc
     2461 cggcggggtg gactggtcca ccggctccag ccagatatcg tcgccttcca catacgcgat
     2521 ctgggtgccg tctggagaaa gcgccgcgaa gggacgcgtg ctgaggactt gcaggtcgcc
     2581 gtatgcgccg aaggttgcca gctccaaccc ggcgcctttc gtcaccacc
//
LOCUS       DATMXK010000068         2392 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_122652, whole genome shotgun sequence.
ACCESSION   DATMXK010000068 DATMXK010000000
VERSION     DATMXK010000068.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2392)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2392)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2392
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122652"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /locus_tag="VN363_01280"
     CDS             <1..561
                     /locus_tag="VN363_01280"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWQ83164.1"
                     /translation="GANLSEAEKPLVLTANGVRFGIVSLGQIEPMAFAGTSQSGIAVL
                     TEESLRAAVTAARQVANVVIVMPHWGPEDVPIPNWSQRSLAEVAVKAGADLVVGNHTH
                     VVQAVQVIQGVPVFYGLGNFVFDQTWALDHMQGVMLQVRFSGPDLLDYELIPTHVDGD
                     GAVHIAGAEEAAQILERIEAASRNLH"
     gene            complement(558..1031)
                     /locus_tag="VN363_01285"
     CDS             complement(558..1031)
                     /locus_tag="VN363_01285"
                     /inference="COORDINATES: protein motif:HMM:NF013629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fur family transcriptional regulator"
                     /protein_id="HWQ83165.1"
                     /translation="MQNVFTSTENDSHQWLVCLQENGYRLTEPRRAVVAAIASSQQAL
                     NPFDVFEKARSNYPRLGLVTVYRTLEKLEELGLIQRVHQPSGCQAFVTAFSGHQHLLI
                     CQRCGRVEFFDGDVEKMDGLMHVVAQNSGYQLQSHWLQLFGVCGNCQQRASRDAN"
     gene            complement(1035..1892)
                     /locus_tag="VN363_01290"
     CDS             complement(1035..1892)
                     /locus_tag="VN363_01290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560494.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_process: GO:0030001 - metal ion transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metal ABC transporter permease"
                     /protein_id="HWQ83166.1"
                     /translation="MIDFLLQPLQYAFMVRGLIAALLTGIVCATVGSYVVLRGMAFFG
                     DALAHAMLPGVAIGYLVSSGAREPIFWWALLTAVLSSLGIGWISRSAEIREDTAIGIV
                     FSGMFALGIALISTVRNYTTDLTHFLFGNVLGVGTNDLLLIGIFGAAILAIIFAFYKE
                     FLVLSFDPLLAATLRLPARSLEYLLLILIALTIVVSLQTVGVALMVAMLVTPAATAYL
                     LTNRLPYMMGLAVLIASLSGIIGLYFSFYLSISSGAAIVLTATAIFLLVWLAKRLADA
                     AAKRTPLKA"
     gene            complement(1885..>2392)
                     /locus_tag="VN363_01295"
     CDS             complement(1885..>2392)
                     /locus_tag="VN363_01295"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="metal ABC transporter ATP-binding protein"
                     /protein_id="HWQ83167.1"
                     /translation="DVVMMGRIGKLGLFRWPQTRDWEIVHQALDLVGLGRLSKRQISE
                     LSGGQQQRMFIARALAQEAELMLMDEPFTGLDIISQQEILNIINELQRRSVTILASTH
                     DLQLAQDSFDRILLLNRQMIGFGPPAEVLAAPQLLEAYGGHLRLVNDGGTLLALGDTC
                     CDGEEPHD"
BASE COUNT          563 a          653 c          692 g          484 t
ORIGIN      
        1 ggagcaaatt tgagcgaggc ggaaaagccg ctcgttctca ctgccaatgg tgtgcgtttc
       61 gggattgtat ctctgggaca gatcgagccg atggcttttg ccggaacaag ccagtctggg
      121 atcgccgtgc tgaccgagga gagcttgcgt gctgcagtaa ccgctgcccg ccaggtcgct
      181 aatgtcgtga ttgttatgcc gcattggggg cctgaggacg tgcccatacc gaattggtcg
      241 caacgcagtc tggcggaggt tgctgtaaaa gccggggcag atctggtggt ggggaaccac
      301 acccacgtcg tccaggctgt tcaggtgatc cagggtgtgc cggtttttta tggcctggga
      361 aattttgtct ttgatcagac ctgggcgttg gaccatatgc agggtgtgat gttacaggtt
      421 cgcttttcag gcccggattt gctcgattac gagctaatcc ccacccatgt cgatggagat
      481 ggcgcggtcc atattgctgg agcagaagaa gccgcccaaa tcctggagcg aattgaggct
      541 gccagtcgca atttgcacta gttagcgtct ctggaagccc gctgttgaca gtttccgcac
      601 actccaaata actgcagcca gtgactctgg agctgataac cgctgttttg ggctacgacg
      661 tgcatcaaac catccatttt ctcaacatcc ccatcgaaaa actcgaccct tccgcagcgc
      721 tggcaaatca gcaagtgctg atgtcctgaa aaggcagtga caaacgcctg gcagccgctg
      781 ggctgatgca cgcgctgaat caatcccagc tcttccagtt tttcgagcgt ccggtagact
      841 gtcaccaggc ccagtcttgg gtaattactg cgggctttct cgaacacatc gaagggattt
      901 aaggcttgct ggctgctggc gatggccgcg actacagccc ggcggggttc tgtgagacga
      961 taaccatttt cttgcaagca gaccagccac tggtgagagt cgttttcggt cgaggtaaat
     1021 acgttttgca tagttcaggc cttgagtgga gtccgcttcg cagccgcgtc cgccaggcgt
     1081 tttgccagcc agaccagcag gaagatggcg gttgctgtga gcacgatagc cgcgcctgaa
     1141 gagatactca aatagaacga gaaatacagg ccaatgatgc cagacaggga ggcaatcagg
     1201 accgccagcc ccatcatgta gggtaagcgg ttggtcagta ggtaagcggt cgctgcagga
     1261 gtaaccagca tggcaaccat caaagcgaca cccaccgttt gcagggaaac tacaatggtc
     1321 aaagcaatta agatcagcag cagatattcc aggcttcggg cgggcaggcg caacgtggct
     1381 gccaggagag gatcgaacga cagcaccaga aactctttat aaaaagcgaa gatgatcgcc
     1441 agaatggctg ccccgaatat cccgatcaac agtaaatcgt tggttccgac cccgagtaca
     1501 ttcccaaaca gaaaatgggt cagatcggtg gtgtaattac gcaccgtcga gatcagggcg
     1561 atacccaggg caaacatgcc cgaaaagaca atcccgatgg cggtgtcctc ccgtatttcg
     1621 gccgagcggc tgatccagcc gatccccagt gagctgagca ctgcggtgag cagcgcccac
     1681 cagaagatcg gctcgcgagc gccactgctg acgagatagc caatggcaac ccccggcagc
     1741 atagcatggg caagtgcatc cccgaaaaag gccatcccac gcagaacaac gtaagatccc
     1801 acggtcgcac aaacgatccc ggtgagcaga gctgcgatca acccgcgcac catgaaagca
     1861 tactgcaagg gttggaggag gaaatcaatc atggggctct tctccatcac aacaagtgtc
     1921 accaagggcc aggagggtcc cgccgtcatt caccaggcgc aggtggcctc cataggcttc
     1981 cagcaactgc ggggctgcca gtacctcggc tggtggccca aacccgatca tctggcggtt
     2041 gagcagcagg atgcggtcga aggaatcctg ggctaattgt agatcgtggg tcgaagcgag
     2101 aatagtgaca ctgcggcgtt gtaattcgtt gatgatgttc aggatttcct gttgggagat
     2161 gatatccaga cctgtgaaag gctcatccat cagcatcagc tcagcctctt gagccagggc
     2221 gcgggcgatg aacatacgct gctgctgtcc gccactcagc tcgctgatct ggcgtttcga
     2281 aagcctgccc agcccgacca gatccagcgc ctggtggaca atttcccaat cgcgagtttg
     2341 gggccagcgg aagagcccca gtttgccaat ccgtcccatc atgaccacat cg
//
LOCUS       DATMXK010000069         4809 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_122630, whole genome shotgun sequence.
ACCESSION   DATMXK010000069 DATMXK010000000
VERSION     DATMXK010000069.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4809)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4809)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4809
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122630"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..359)
                     /locus_tag="VN363_01300"
     CDS             complement(<1..359)
                     /locus_tag="VN363_01300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83168.1"
                     /translation="MAKFDETPIVKDPAVEGLLVPPPLREHIAREVRCWPSKEKDTHK
                     INLPATLVPDWQEKAIQKFAELLDKNKALRVYMDICVRCGACTDKCQFFLGTGDPQNF
                     PVARAELMRKVYRRYFTP"
     gene            complement(401..1153)
                     /locus_tag="VN363_01305"
     CDS             complement(401..1153)
                     /locus_tag="VN363_01305"
                     /inference="COORDINATES: protein motif:HMM:NF014695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="respiratory nitrate reductase subunit gamma"
                     /protein_id="HWQ83169.1"
                     /translation="MESSPVIWSLLSYFLIGLAYFAATVFVAGLLSKLIGYLRTPMPW
                     PETTTPAPTTEGGAVLRVLGDVTIFPNLFKADKWLWAGAWLFHAGLAAVLFRHMRYFT
                     YPVPGLVLAMEPAALFFGYLLGGAALFLFFRRLVLPRTLYISNIPDFFALALLGGIAA
                     TGILMSTWIKVYLVDVKAFMLGLITLNPVLPPQHPLFLFHFFLVLLLLIYFPFSKLLH
                     AGGIFFSPSRNLPFEVQVAGKRHVNPWDKPAR"
     gene            complement(1375..1692)
                     /locus_tag="VN363_01310"
     CDS             complement(1375..1692)
                     /locus_tag="VN363_01310"
                     /inference="COORDINATES: protein motif:HMM:TIGR03342.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TusE/DsrC/DsvC family sulfur relay protein"
                     /protein_id="HWQ83170.1"
                     /translation="MPVMDVLGSQIIVDEDGFLEDPDVWSQDLALAIAQMEDVPELTE
                     DHWKVINYLRDYYKQFGIAPMIRKLCKDTGFPLKDIYELFPSGPAKGACKIAGLPKPT
                     GCV"
     gene            complement(1759..1968)
                     /locus_tag="VN363_01315"
     CDS             complement(1759..1968)
                     /locus_tag="VN363_01315"
                     /inference="COORDINATES: protein motif:HMM:NF020263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory sulfite reductase D family
                     protein"
                     /protein_id="HWQ83171.1"
                     /translation="MSDMPMEELKAKVLEYFKAKGKMMTSRDVAKGLELENRLVKLVL
                     TELVNEGTLEFTSYGGATFIKLPEN"
     gene            complement(1981..3042)
                     /gene="dsrB"
                     /locus_tag="VN363_01320"
     CDS             complement(1981..3042)
                     /gene="dsrB"
                     /locus_tag="VN363_01320"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02066.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     beta"
                     /protein_id="HWQ83172.1"
                     /translation="MATRTDFGPPNYKDLLPPVIEKNYGQWKYHERPQPGVLRHVAES
                     GEELYTVRVGSPRLVSTDWIKDMCEIADEYCGGYLRFTSRHNLEFLVSDKSKVEPLIA
                     YLKSIGLPVGGTGHAISNPVHTQGWIHCHTPATDASGIVKAVMDDLYEYFVDMKLPAY
                     CRISLACCLNMCGAVHCSDIAILGIHRMPPKINHDIVQAKCEIPNVIAACPTGAIRPD
                     PKNKSLTVNVDRCMYCGNCYTMCPGMPLADPIGDGVSIWVGGKISSARTPPKFSRLVI
                     PFLPNNPPRWPEVTSAVRNIVEVWASRAHKHERMGEWIERIGWERFFELSGIPFEVQL
                     IDDFTLASDTYRSTPSFKW"
     gene            complement(3042..4220)
                     /gene="dsrA"
                     /locus_tag="VN363_01325"
     CDS             complement(3042..4220)
                     /gene="dsrA"
                     /locus_tag="VN363_01325"
                     /EC_number="1.8.99.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02064.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018551 - dissimilatory sulfite reductase
                     activity [Evidence IEA];
                     GO_process: GO:0019420 - dissimilatory sulfate reduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dissimilatory-type sulfite reductase subunit
                     alpha"
                     /protein_id="HWQ83173.1"
                     /translation="MSKTPMLDELEQGPWPSFVSEMKRAAEKKDSAKDLINQLERSYE
                     DRIGHWKHGGIVGVKGYGGGVIGRYSDMPEAFPAVAEFHTLRVNQPSGWFYNTETLRK
                     LCDVWDKHGSGLTNMHGATGDIILLGAPTSALQPCFDDLAEIGFDLGGSGSDMRTPSC
                     CVGPGRCEYACIDTLDLLYDITMEYQNELHRPMFPYKSKIKVSGCANDCVAAVPRSDI
                     AVIGTWRDNIQVDQNCVAEYAAEGFDISGEVVALCPTRCLVYDSAAKTLTIDDKECNR
                     CMHCLNLMSKALHIGKERGATVLVGGKAPIVHGAMLSHVIVPFIKMEPPYAEFKELLE
                     KIWDWWDENGKMRERLGELINRLGMRAFLKAIDLEPSPQMIKVPRANPYYFWDQEEVK
                     "
     gene            complement(4417..>4809)
                     /locus_tag="VN363_01330"
     CDS             complement(4417..>4809)
                     /locus_tag="VN363_01330"
                     /inference="COORDINATES: protein motif:HMM:NF015558.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative sulfate exporter family transporter"
                     /protein_id="HWQ83174.1"
                     /translation="VKQTQNALIGFVAFFLALYFATVVEGKKEKPSASIIWKRFPKFV
                     LGFIVASLLFTFGVIQKGDGATAISSLKDWAFCLAFVCMGLELSVSEFKKMGWSPVVV
                     YLIVTVFNTLLALVVSWVIFGWLFPPTL"
BASE COUNT         1103 a         1339 c         1372 g          995 t
ORIGIN      
        1 ggggtaaagt agcgccggta tactttgcgc atcagctcag cccgcgccac ggggaagttt
       61 tgcgggtcgc cggtgcccag gaaaaactgg catttatcgg tgcaggcgcc gcagcgcacg
      121 cagatatcca tatagacccg caaggcctta ttcttatcca gcagctcggc gaatttctgg
      181 atcgcttttt cctgccagtc cggcaccagg gtggcaggca ggttgatttt gtgggtatct
      241 ttttctttgc tgggccagca gcgcacctcg cgggcgatat gctcacgcag cggcgggggg
      301 accagcagac cttcgaccgc cgggtctttt acgataggag tttcgtcaaa tttggccata
      361 tagattccta tagctctgcg agccagggtg gctagcctgg ctagcgcgcc ggtttgtccc
      421 agggattgac gtggcgtttc ccggcaacct gaacctcgaa gggtaaattg cgcgaggggc
      481 tgaagaagat cccgccagcg tgcagcagct tgctgaaggg gaagtagatc aacagcagca
      541 ggaccaggaa gaaatgaaac aggaacaggg gatgctgcgg gggtagaact ggattcaagg
      601 tgatcagacc cagcatgaat gccttaacat ctaccaggta aaccttgatc caggtgctca
      661 tcaggatgcc ggtcgcggcg atcccgccca gcagcgccag ggcgaaaaag tcgggaatat
      721 tggagatgta cagggtgcgc ggtaaaacca ggcgccggaa gaatagaaat aaagccgccc
      781 cacccaaaag gtaaccaaag aaaagagctg ccggctccat agccaaaacc agcccgggga
      841 ccgggtaggt gaaataacgc atatggcgga acaggaccgc tgccagaccg gcatggaaga
      901 gccaggcgcc ggcccacagc catttatcag ccttgaacag gttggggaag atggttacat
      961 cccccagaac gcgcaggacc gccccgcctt cagtggtcgg cgccggcgtg gtagtttcgg
     1021 gccagggcat gggagtccgc agatacccga tcagcttgct caataaacct gccacaaaga
     1081 ctgtggcggc gaaataagcc agaccgatga gaaaatagga taataatgac cagatgaccg
     1141 gtgagctctc catcagcgct ccttaaggtt tctgcaaatt acgcctgctg ttctctgaac
     1201 aagcggagga gcgatcagcc tcagatagga ttgatgcggg caggcgtgca gggtgctgca
     1261 cctgaggttg tttcgctcct ccccaaatga ctatggcttt gttctgcctg ctggcctgag
     1321 ccgcaccccc tccaagcggc ctcccagccg gcagccccct gagaaaatcc gttcctagac
     1381 acatccggtc ggtttgggta agccggcaat cttacaggcc cctttggcag gaccgctagg
     1441 aaataactcg taaatatcct tgagcgggaa accagtatcc ttgcataact tgcgaatcat
     1501 cggggcgatc ccgaactgct tgtaataatc gcgcaggtag ttgattacct tccagtgatc
     1561 ctcggtcagc tcgggaacat cttccatctg ggcaatcgcc agcgccagat cctggctcca
     1621 gacgtctgga tcttccagaa agccatcttc atcaacaata atttgtgaac ccaatacatc
     1681 cataacaggc atttttaact ccttgggtgg ttctccaggt ccggaagctt tcccaccaga
     1741 cctggagaat tgattggcct agttttcggg cagcttgata aaggtcgccc caccataaga
     1801 ggtgaactcg agggtgcctt cgttcaccaa ttcggtcagg accagcttca ccaggcggtt
     1861 ttccagctcg agacctttcg caacatcccg gctggtcatc atcttgccct tggctttgaa
     1921 gtactccagg accttcgcct tgagctcttc cattggcatg tcactcatta gaaacctcgc
     1981 ttaccatttg aaagatggtg tcgagcgata ggtatcggac gccagagtga aatcatcgat
     2041 gagctggacc tcgaacggga taccggacag ctcgaagaaa cgctcccagc cgatgcgctc
     2101 gatccactcg cccatgcgct cgtgcttgtg ggcgcggctg gcccagactt caacgatatt
     2161 gcgcactgcc gaggtcactt cgggccagcg agggggattg ttaggcagga aggggataac
     2221 caggcgggag aacttgggag gtgtacgggc gctggaaatc ttgccgccca cccagattga
     2281 gacgccatcc ccaatcggat cggcaagggg catccccggg cacatggtgt agcagttgcc
     2341 gcagtacata cagcggtcaa cattcaccgt cagggattta ttcttgggat cagggcggat
     2401 agcacccgtg gggcaggctg cgatcacgtt ggggatctca catttcgcct ggacgatatc
     2461 atggttgatt ttcggcggca tgcggtggat tcccaggatg gcaatgtctg agcagtggac
     2521 cgcgccgcac atattcaggc agcacgccag ggagatgcgg cagtacgccg ggagcttcat
     2581 atccacgaag tactcgtaca ggtcatccat gaccgctttg acgattcctg aagcatcggt
     2641 ggcgggggtg tggcagtgga tccagccctg ggtgtggacc ggattgctaa tggcatggcc
     2701 tgtgccgccc actggcagcc cgatggactt gaggtaggca atcaggggtt cgaccttgct
     2761 cttatccgag accaggaact cgaggttgtg gcggctggtg aagcgcaaat aaccgccgca
     2821 gtactcatcg gcgatctcgc acatatcctt gatccagtcg gtgcttacca ggcgcggcga
     2881 gccaacccgc acggtgtaaa gctcctcgcc agattcggcg acatggcgca gcacacccgg
     2941 ctgcgggcgc tcgtggtact tccactgccc gtagttcttc tcaattactg gtgggagcag
     3001 gtccttgtaa tttggaggac cgaaatctgt tcgcgttgcc attacttgac ctcctcttga
     3061 tcccagaagt aatagggatt ggcgcggggt accttgatca tctgcgggga aggctcaagg
     3121 tcgatcgcct tcaggaaagc gcgcattccc aggcggttga tcagctcacc caggcgctcg
     3181 cgcatcttgc cgttctcatc ccaccaatcc cagatcttct ccaggagctc tttgaactcg
     3241 gcgtaaggcg gttccatctt aatgaaaggt acaatcacat gcgagagcat ggcgccatgg
     3301 acgatagggg ctttgccgcc taccagaacc gtagcgccgc gttctttacc aatgtgcaga
     3361 gccttagaca tcaggttcag gcagtgcata cagcgattgc attctttatc gtcgatggtc
     3421 agcgttttcg ccgcgctatc gtaaaccagg catcgggtgg ggcacagagc caccacttcg
     3481 ccggagatat caaatccctc cgcggcatat tcggccacac agttctgatc tacctggata
     3541 ttgtctcgcc aggtgccgat caccgcgata tccgagcgtg ggacagccgc tacgcagtcg
     3601 tttgcacaac ccgagacttt aattttggat ttgtagggga acatcggacg gtgaagctcg
     3661 ttctgatatt ccatcgtgat atcgtaaagc agatcaaggg tgtcgatgca ggcatactca
     3721 cagcgcccgg gaccgacaca gcagctggga gtgcgcatgt cgctgcccga gccgcccaga
     3781 tcaaagccaa tttcagccag gtcatcaaaa caaggctgca aagcgctggt gggagcgcca
     3841 agcaggatga tgtcaccggt cgccccgtgc atattggtga ggccggagcc gtgtttatcc
     3901 cacacatcac agagcttgcg cagtgtttca gtattgtaga accaaccaga aggctggttg
     3961 acccgcaggg tgtggaattc cgccactgcc gggaaagctt caggcatatc tgaatagcgg
     4021 ccgatcaccc cgccgccata gcctttgaca cccacgatac cgccatgctt ccagtgacca
     4081 atgcggtcct cgtaggaacg ttccaattga ttgatcaagt ctttggccga atctttcttc
     4141 tcggcagccc gcttcatttc agagacgaag ctgggccagg gaccctgttc gagctcatca
     4201 agcattgggg tctttgacat gaggtacacc tccaaaagag taattgaccg gtcgtgccag
     4261 aggttttcat tcaggcaggc agagctgctt tgcgagccct gcctgccagt ttggcgccga
     4321 aaagcacctg cgcaagaatt gacctgaaaa ggtagcctgg gtgaccctga ggcagcgacg
     4381 ggagcccggg acaatccctg cgcaggcttc aaaaacctag agagtcggcg ggaataacca
     4441 gccgaagatc acccaggaga ccacgagggc cagcagggta ttgaacacgg ttacgatcaa
     4501 ataaacgacg accggcgacc atcccatctt cttgaattcg ctcacggaga gctccaggcc
     4561 catgcatacg aacgccaggc agaaggccca gtctttcagc gaggaaatcg cggttgctcc
     4621 atcacccttc tggatcacgc cgaaggtgaa cagcaaggaa gcgacgatga aacccaaaac
     4681 gaacttgggg aagcgcttcc agatgatcga ggcagacggt ttttctttct tgccttccac
     4741 caccgtggcg aagtacagcg ccaggaagaa agctacgaaa ccgatcaggg cattttgggt
     4801 ctgcttcac
//
LOCUS       DATMXK010000070         2876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_19298, whole genome shotgun sequence.
ACCESSION   DATMXK010000070 DATMXK010000000
VERSION     DATMXK010000070.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2876
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19298"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            406..1188
                     /locus_tag="VN363_01335"
     CDS             406..1188
                     /locus_tag="VN363_01335"
                     /inference="COORDINATES: protein motif:HMM:NF012455.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015267 - channel activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MIP/aquaporin family protein"
                     /protein_id="HWQ83175.1"
                     /translation="MKMKELIGEVFGTFILILLGDGVVANVGLAPRLAAPGYNWNTIT
                     IGWAFAVIVAVYVSGGVSGAHLNPAVTLGLAVKRGFPWGKVFPYILAQLVGAFLGALA
                     VYLVYMDGLRAAGMPNVWSTGPGSVFGASFWGGEGAAATGTYSLLNACIAEFFGTMML
                     MWGIAASGDSRNMGLMHNMGPFLVGFTVLAVGLSLGGPSGYSINPARDLGPRILGTLV
                     GTQGLFDGIYWLLPPVLIPAIAGPIGFMVYDWFVTAQLPEKK"
     gene            1250..2251
                     /gene="dhaK"
                     /locus_tag="VN363_01340"
     CDS             1250..2251
                     /gene="dhaK"
                     /locus_tag="VN363_01340"
                     /EC_number="2.7.1.121"
                     /inference="COORDINATES: protein motif:HMM:TIGR02363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaK"
                     /protein_id="HWQ83176.1"
                     /translation="MKKLINQPEDVVVEELQGIAYAHGDLVKVHYNPNFIVRADAPVK
                     GKVGVISGGGSGHEPMHGGFVGMGMLDAACPGAVFTSPTPDQMLEATKAVNGGAGVLH
                     IVKNYTGDIMNFEMAAELARAEGIDVEAVITNDDVAVQDSLYTAGRRGVGITVIAEKI
                     SGAAAEQGRSLKEVADICRKVNAQGRSMGMALTSCTVPHAGKPTFDLPEDEMEIGIGI
                     HGEPGRVRMKLKSADEITEMLMEPIVSDLPYKSGDEVLLFVNGMGGTPLLELYVIYRK
                     AYEIAEKHGLKVVRNLIGPFITSLEMAGTSITLLKMDSDMVKLWDAPVKTPGLRWGV"
     gene            2333..>2876
                     /gene="dhaL"
                     /locus_tag="VN363_01345"
     CDS             2333..>2876
                     /gene="dhaL"
                     /locus_tag="VN363_01345"
                     /inference="COORDINATES: protein motif:HMM:TIGR02365.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydroxyacetone kinase subunit DhaL"
                     /protein_id="HWQ83177.1"
                     /translation="MSISRDEILAWLQDVAQTIAENRDYLVQLDSPIGDADHGANMDR
                     GFRAIMGKMGEISDKDIGTIFKTAAMTLIATVGGASGPLYGTFFLQAGTKTTGKMEIT
                     IEEWAVVLEAALAGIVMRGKAEVNQKTMVDALTPAVQALKQAVADGKTQAEALELSAE
                     AARQGMLATIPMLALKGRASY"
BASE COUNT          636 a          762 c          793 g          685 t
ORIGIN      
        1 gccggacgac cctttggcgt aagctgcgcg aatttgagat ctcggtcgat gatttccgca
       61 gctcaaaaat ataatttagg gagaataaat ttcctccggt aagtgtttca caacgaaaca
      121 actccccgta gaattgtttc acttctgtac gttttttcca cctgggttga cagaaatgaa
      181 agccgtgtca taatgaatgg tatgtgtgtg tcaacgtgta cccttacctg tacgcgcggg
      241 cgcatgaccg aacaggcaat catcatggca gaatccttat gctgtttgct ataggatcgg
      301 gaaaggaggg ctacccagat agatttgatc atttcgttgt tcggatctcc ctaacaagtt
      361 cgaccgaatt tttgatatta agtcttattc caaggagaat gaaccatgaa aatgaaagag
      421 ttgatcggcg aggtctttgg cacattcatt ctgatcctgc ttggtgacgg cgttgttgcc
      481 aatgttggtc tggcccctcg tctggctgcc cctggctata actggaatac catcaccatc
      541 ggatgggcct tcgcagtgat cgtcgccgtc tatgtctccg gcggcgtttc tggcgctcac
      601 ttgaacccgg ccgtcaccct tggcctggct gtcaagcgcg gcttcccctg ggggaaagta
      661 ttcccctaca tcctcgccca gttagtaggc gccttccttg gcgccctggc tgtatacctg
      721 gtctacatgg atggtctgag ggctgctggt atgcccaatg tctggtccac cggtccaggc
      781 tcagtatttg gcgcatcctt ctggggtggg gaaggcgctg ccgcgacggg tacttactca
      841 ctgctgaatg cctgcatcgc agaattcttt ggcacgatga tgctgatgtg gggcatcgcc
      901 gccagcggcg actctcgcaa catgggcctg atgcacaaca tgggaccttt cctggtcggc
      961 ttcaccgtcc tggctgttgg tctttctctg ggtggcccca gcggctactc aatcaacccc
     1021 gcccgtgacc tcggccctcg catccttggc actctcgttg gcacacaagg cctgttcgac
     1081 ggcatctatt ggctgctccc gccagtcttg atcccggcga tcgctggccc gatcggcttt
     1141 atggtttacg actggtttgt caccgctcaa cttcctgaga agaaataata accctggtgc
     1201 atttttacgt agcagggctt ttttttacaa ggaatgagga attagagcga tgaagaaatt
     1261 aattaaccag cccgaagatg tcgtcgttga agagctccag ggcattgctt atgcccatgg
     1321 ggatcttgtg aaagtccact ataacccgaa ctttatcgtg cgcgccgatg cgccggtgaa
     1381 gggcaaagtg ggcgtgattt ctggcggtgg ctccggccac gaacccatgc acggcgggtt
     1441 tgtgggtatg ggcatgctgg atgcagcctg ccccggagca gttttcacct caccgactcc
     1501 cgatcaaatg ctggaagcga ccaaggctgt caatggtggc gcgggcgttc tacacatcgt
     1561 caagaactac accggcgaca tcatgaactt cgaaatggct gccgagctgg ctcgcgctga
     1621 aggcatcgat gtcgaggctg tgatcaccaa cgacgatgtt gctgtgcagg attccctgta
     1681 tacagctggg cgtcgcggcg tgggcatcac agtcatcgct gaaaagatca gcggtgcagc
     1741 tgccgaacaa gggcgctctc tcaaagaggt ggccgatatc tgccgcaagg taaacgcaca
     1801 gggccgctca atgggcatgg cgctcacctc ctgcacggtt cctcacgccg gcaaaccgac
     1861 cttcgatctg ccggaagatg agatggaaat cggcatcggc atccatggcg agcccggacg
     1921 tgtgcgcatg aagctcaaaa gcgctgacga gatcactgaa atgttgatgg aacctatcgt
     1981 cagcgacctg ccttacaaga gtggggacga agtcctgctt tttgtcaacg gtatgggcgg
     2041 cacgcccctc ctcgagcttt atgtgatcta tcgcaaggct tacgagattg ctgagaagca
     2101 tggtctcaaa gtcgtccgca acttgattgg tccattcatc accagccttg aaatggctgg
     2161 cacctcgatt acactgctca agatggatag cgacatggtc aagctctggg atgcacctgt
     2221 caagactcct ggcttgcgct ggggggttta agacaactga ataactaaat tcagagggtt
     2281 ggcggtggaa tacctgccaa ccctctgaaa tatccttatg taggggtccc ctatgtccat
     2341 atcgcgagat gagattcttg cctggctgca agatgttgcc caaaccatcg ccgaaaaccg
     2401 ggattatctg gttcaactgg actcaccgat cggtgacgct gaccatggcg cgaacatgga
     2461 tcgtggcttt agagccatca tgggcaaaat gggagaaatc agcgataaag acattggcac
     2521 catctttaaa accgctgcta tgacattaat tgctaccgta ggtggcgcca gcggcccact
     2581 ctatgggaca ttcttccttc aagcgggtac caaaactacc ggtaagatgg aaatcaccat
     2641 tgaagaatgg gcagttgttt tagaggctgc tctcgcgggg attgtcatgc gcggtaaagc
     2701 cgaagtgaac caaaaaacga tggtcgatgc cctcactccg gctgtccagg cattgaaaca
     2761 ggctgtcgca gatgggaaga cgcaagctga agctttggag ctgtctgcgg aggcagcccg
     2821 ccagggaatg cttgccacga ttcccatgct cgctctcaaa gggcgcgcca gctacc
//
LOCUS       DATMXK010000071         5811 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_122498, whole genome shotgun sequence.
ACCESSION   DATMXK010000071 DATMXK010000000
VERSION     DATMXK010000071.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5811)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5811)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5811
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122498"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..688)
                     /locus_tag="VN363_01350"
     CDS             complement(<1..688)
                     /locus_tag="VN363_01350"
                     /inference="COORDINATES: protein motif:HMM:NF024793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ83178.1"
                     /translation="MDGPGAKTIPILRTKITLPRPHPETTPRRSLEARLVSALGGKLV
                     LVSAPAGYGKTTLLTRVLGDADIPVAWLSLDENDDQPQRFWSNVISALQAVLPGSCGQ
                     AQALLGTPQPPPFELVLVELVNDLVYSGQELILVLDDLHEICSAEILGNLAALIEKMP
                     PNLHLILSSRQTPTLQLARLRARRELAEFTAADLRFQPPEIERLFNELFTLDLSAGDL
                     DTLENLTEGWI"
     gene            complement(705..2201)
                     /locus_tag="VN363_01355"
     CDS             complement(705..2201)
                     /locus_tag="VN363_01355"
                     /EC_number="3.4.11.1"
                     /inference="COORDINATES: protein motif:HMM:NF002074.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070006 - metalloaminopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0019538 - protein metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="leucyl aminopeptidase"
                     /protein_id="HWQ83179.1"
                     /translation="MEIRTTVGTLDTQQVDTWVVGVFEGAQELKAAAGQVDAALDGGL
                     LALLQSGEFTGKAGEVIVVYTFGKVPAGRVLLVGLGKEAEFDLEAVRRASGAAVNRGR
                     DLGAQALASELFGAGRSSYSTADVAQAMAEGGMLALYDYRARFNQKPPRNNPQTLVLV
                     AGSPAEQAALEEGVHFAQASVPGVVLARDLVNLPPNTATPTYLSTVAEEIAQEYGMKL
                     TVGDREWAQAMNMGAFLCVARGAEEPPKFIVLEYNAERAELPTVVLVGKGITFDSGGI
                     SIKPSDKMDEMKSDMAGAAAVLAVMKSAGELKLPLHLVGITPCTENMPDGRASRPADV
                     VTASNGKTIEIISTDAEGRLILADALVYASRYNPAVVIDLATLTGACVIALGDNIAAG
                     LFCNDDELSQRLVESGSKTFERLWPMPLWDDYKKAIESRYADIKNTGGRYGGVGTSAI
                     FLKQFIDYRWAHLDIAGMALADKELPYQPAGGTGYGVRLLLDYLRNYH"
     gene            complement(2245..4380)
                     /locus_tag="VN363_01360"
     CDS             complement(2245..4380)
                     /locus_tag="VN363_01360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293345.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005829 - cytosol [Evidence IEA];
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0003729 - mRNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Tex family protein"
                     /protein_id="HWQ83180.1"
                     /translation="MDAALLISNALSLRQESVAAAIALLDEGNTLPFIARYRKEATGG
                     LDDEQLRELESRLGKLRALDERRQTILGSIQEQDKLTPELAAQIQAAETLTALEDLYL
                     PYKPKRRTRASLAREKGLEPLADLILAQGRGDVSPEALALSFLSEAVPGIEEALAGAR
                     DIVAETISDHAEVRRQTRERAFLHGSLLCEKVPGAEDPRRVYELYYEYQQQVERLRPH
                     QILALDRGEREKVLRIKLIVPDRDWLGAIQSIFRPDPRSPFAGELQLAIVDAAARLLL
                     PAIERDVRRALTEKAETHAIGVFTTNLRALLTQPPLAGHTIIGLDPGFRTGCKVAVVD
                     PTGKVLWTGVIYPHEPQKQWAAAVRTLLELVDRFHASLISIGNGTASRETEKLAAEVV
                     QAAPEVKYLITNEAGASVYSASELARAELPGMDVSLRGAVSIARRVQDPLAELVKIDP
                     KSIGVGMYQHDVDQKNLSQALSGTVEGVVNHVGVDLNTASPALLTYIAGIGPRLAGSI
                     VTSRDTNGPFLDRQALRKVSGLGPKAFEQCAGFLRIRGGSQPLDATHIHPESYAVAEK
                     LLQMAGVSARQPQAEIQEHLDRLLLSKPLPRLAAELKCGEPTLKDILEQLARPGRDPR
                     QDFPAVLLRSDVLVVEDLQPGMHLKGTVRNVVDFGAFVDIGVKQDGLLHRSRIPQTAL
                     LRVGDIIEVEILSIEPDRGRISLGWTTPG"
     gene            complement(4388..4897)
                     /locus_tag="VN363_01365"
     CDS             complement(4388..4897)
                     /locus_tag="VN363_01365"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWQ83181.1"
                     /translation="MTFSDLIARRYSVRAYRSNPVEAEKLNAILEAGRMAPTASNRQA
                     FGIIVARPATNPEKFHQLYHREWLLQAPYLICICTLTAQSWANKESKSYADVDAALVV
                     DHMVLQAAELGLGTCIIANFDPPTASELFSLPKGVEPVLMFTLGYPADQPKTKVRKPL
                     AELVHEGHW"
     gene            5032..5382
                     /locus_tag="VN363_01370"
     CDS             5032..5382
                     /locus_tag="VN363_01370"
                     /inference="COORDINATES: protein motif:HMM:NF014664.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051920 - peroxiredoxin activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carboxymuconolactone decarboxylase family
                     protein"
                     /protein_id="HWQ83182.1"
                     /translation="MADRVDEFQAYRTRMNERIFDLDHLGIKRFFNLDTAAYRDGALD
                     PKTKELLGLVASTVLRCNDCIDYHLIQCREAGWKDEELYDALNVALIVGGSIVVPHLR
                     HAVETIDILHEKSE"
     gene            5390..>5811
                     /locus_tag="VN363_01375"
     CDS             5390..>5811
                     /locus_tag="VN363_01375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83183.1"
                     /translation="MEYLTFDALQQQLLSLFKAGDFEAVRQLAAEQIVQYPEQFHILA
                     YWQITAAARQMEYPAALQLIRGLLERGFWYGESVLVNSPSLQPLQDMPEFINLLEASR
                     ALRARSEKDLYPLLILRQPERGEKGGPPTPLMIALHTNG"
BASE COUNT         1135 a         1778 c         1731 g         1167 t
ORIGIN      
        1 cgatccagcc ttcagtcaga ttttccagag tatccagatc gcccgccgag agatcgaggg
       61 taaacagctc gttgaacaag cgctcgatct cagggggttg gaagcgaagg tcggcggcag
      121 taaattctgc cagctcgcgc cgggcgcgca gccgggccag ctggagtgtg ggggtctggc
      181 ggctggaaag gatcaaatgc agattgggcg gcattttttc gatcaatgcc gccaggttac
      241 ccaggatttc cgccgagcaa atctcatgca gatcatccag gaccaggatc agctcctgcc
      301 ccgaataaac caggtcgttc accagctcca ccaggaccag ctcaaaaggt gggggttgag
      361 gagtaccgag cagagcctgg gcttgcccac agctgcccgg caggacagcc tgcagggcgc
      421 tgatcacatt actccagaat ctttggggct gatcatcgtt ttcatcgagt gagagccagg
      481 cgaccggtat gtctgcatcg cccaggaccc gcgtgagcag ggttgtcttg ccatagccag
      541 ccggcgcgct gaccagaacc agcttgcccc ccagagcgct gaccaggcgc gcttccagag
      601 accggcgtgg ggtcgtctcc ggatgcggcc tgggcagggt tattttggtg cgcaagatcg
      661 ggatggtctt tgcgccgggt ccgtccatgg cgttgctccg cgatttaatg ataattacgc
      721 aggtagtcca gcagcaggcg cactccgtaa ccggtgccgc cggctggctg gtaaggcagc
      781 tctttatctg ccagggccat cccggcgata tccaggtgcg cccaccggta gtcgatgaat
      841 tgtttcaaga aaatggccga agtgccaacg cctccgtagc gaccgccggt gtttttgata
      901 tcggcgtagc gcgattcgat ggctttcttg taatcatccc ataatggcat cggccaaaga
      961 cgctcgaagg ttttactgcc actttccacc aggcgctggc tgagctcatc atcattgcag
     1021 aacagcccgg cggcgatatt atcgcccagg gcgatcacac acgccccggt cagagttgcc
     1081 aggtcaataa ccacagccgg gttgtagcgg ctggcgtaca ccagagcatc cgccaggatc
     1141 aaacgccctt cggcatcggt gctgatgatc tcgatggtct tgccattcga ggcggtgacc
     1201 acatccgccg gacgggatgc gcgcccgtcg ggcatgtttt cagtgcaggg ggtgattccc
     1261 accaggtgca ggggcagttt cagctcgccg gcggatttca tcaccgccag cacggcagcc
     1321 gcacccgcca tatcggattt catctcgtcc atcttatcac tgggtttgat cgagatcccg
     1381 cccgaatcga aggtgatgcc ctttccaacc agcaccacgg tgggcagctc agcgcgctcg
     1441 gcattgtact cgagcacaat gaacttgggc ggctcttcgg ccccccgcgc cacacataaa
     1501 aacgccccca tattcatcgc ctgggcccat tcccggtcgc cgacagtcag cttcattccg
     1561 tattcttgag caatctcttc ggcaaccgtg gataagtagg tgggtgtcgc tgtgttgggc
     1621 ggaagattga ccagatcgcg cgccaggacc acccccggca cactggcttg tgcaaagtgc
     1681 acaccttcct ccagggcagc ctgttccgcc gggctcccgg caaccagcac cagggtctgc
     1741 gggttgttgc ggggcggctt ctgattgaag cgagcccggt aatcgtataa tgccagcatg
     1801 ccgccttcgg ccatcgcctg ggccacatct gcagtcgaat agctcgacct gcctgcccca
     1861 aacagctcgc tggccagcgc ctgcgcgccg agatcacgcc cgcggttaac tgccgcgccg
     1921 gaggctctgc ggaccgcttc caggtcgaat tcggcttcct tgcccagtcc aaccaggagc
     1981 acgcgcccgg cgggtacctt accaaaggta tagaccacga taacttcgcc ggctttaccg
     2041 gtaaattccc cactctgcag caaggcgagc agcccgccat ccagggcggc atcgacctgt
     2101 cccgcggcag ctttaagctc ctgcgcgcct tcgaacacgc ccacgaccca ggtatctacc
     2161 tgctgagtat cgagcgttcc aacagttgtc ctgatttcca tttctgagtg cctccaaact
     2221 tgggtatgat gcgagccatg cggctcagcc aggggtcgtc caacccaggc tgatccgccc
     2281 gcgatctggc tcgatggata atatctctac ctcgataata tcaccaactc gtaacagcgc
     2341 agtctggggt atccggctgc ggtgcagcaa gccatcctgc ttgactccga tatccacaaa
     2401 tgccccaaaa tcgaccacat tccggacggt gccttttaaa tgcatgccgg gctgcagatc
     2461 ttccacgacg agcacatccg agcgcagaag aaccgccggg aaatcctggc gtgggtcgcg
     2521 gccgggacgg gcgagctgct cgaggatatc tttcaaggtc ggctcgccgc atttcagctc
     2581 ggccgccagc ctgggaagag gtttgctcag cagcaggcga tccaaatgct cctggatctc
     2641 agcctgcggc tgccgggcgc tcacacctgc catctggagc agtttttcgg cgaccgcata
     2701 gctctcgggg tggatatggg tggcatccag gggctggctg ccgccgcgaa tgcgcaggaa
     2761 acccgcacac tgttcgaatg cttttggacc caggccgctg accttgcgca gcgcctgacg
     2821 gtcgaggaag ggtccgttcg tgtcacggct ggtgacgata ctgcccgcca gccggggacc
     2881 gatccccgca atataagtca aaagggccgg tgaagcggta ttcagatcca caccgacatg
     2941 gttgaccacg ccttccacgg tcccactcag cgcctgggac aggttttttt gatcaacatc
     3001 gtgctggtac atcccgaccc cgatcgattt cgggtcgatc ttgaccagct cggccagcgg
     3061 gtcctggaca cgccgggcga tggaaaccgc cccgcgcagg ctgacatcca tcccgggtaa
     3121 ttcagcccgc gccagctcgc tggcactgta cacactggcg ccagcctcgt tcgtgatcag
     3181 gtattttact tccggggcag cctgaacgac ttcggcagcc agtttttcag tctcgcggga
     3241 tgcggtgccg ttcccaatcg agatcaggct ggcatgaaag cggtcgacca gctcgagcag
     3301 ggtcctgact gcagccgccc actgtttttg cggctcgtga gggtaaatta cgccagtcca
     3361 caggacctta ccggtgggat cgaccacggc gactttgcag ccggtgcgaa atcccggatc
     3421 aagcccgatg atggtatgcc ccgccagcgg cggctgggtg agcagggcgc gcaggttggt
     3481 ggtgaacacg ccaatggcat gggtttcggc tttctcggtc agagcgcggc gcacatcccg
     3541 ctcgatcgcc ggcaggagca gccgcgccgc ggcatcgacg atcgccaatt gcagctcccc
     3601 ggcgaaaggc gagcgtgggt cgggacgaaa gatactctgg atggctccca gccagtcccg
     3661 gtcggggacg atcaatttga tgcgcagaac cttttcgcgc tcgccgcgat ccagggccag
     3721 gatctggtgg gggcgcaggc gttcaacctg ctgctggtat tcgtaataaa gctcgtacac
     3781 ccggcgggga tcttcggcgc caggtacctt ttcgcacagg aggctgccgt gcaggaaagc
     3841 ccgctcgcgc gtctggcggc ggacttcggc gtgatcgctg atcgtctctg cgacgatatc
     3901 gcgcgctccg gccagcgcct cctcaatccc aggcaccgcc tcgctcaaga aagacagggc
     3961 cagggcctca ggggatacat ccccacgccc ctgggccagg atcaggtcag ccaggggttc
     4021 caggcctttt tcgcgcgcca ggctggcccg cgtgcggcgc ttcggcttgt agggcaggta
     4081 gagatcttcc agtgcggtca gggtttcggc agcctggatc tgggctgcca gctccggggt
     4141 gagcttgtcc tgctcctgaa tggagccgag gattgtctgg cggcgctcat cgagggcacg
     4201 cagcttgccc aggcggcttt caagctcacg cagctgttca tcatccagcc caccggtagc
     4261 ttccttgcga tagcgggcga tgaagggcag ggtattgcct tcatccagca gggcaattgc
     4321 ggctgccaca ctctcctggc ggagggagag ggcatttgag atcagtaagg cagcatccat
     4381 acggggttta ccagtgtcct tcgtggacaa gctcggcaag gggtttgcga actttggttt
     4441 ttggctggtc tgccggatag cccagggtga acatcaaaac cggctccacg cctttcggca
     4501 ggctaaacag ctcggaggcg gtggggggat caaaattagc gatgatacac gtccccaggc
     4561 ccagttccgc agcctgcaac accatatgat cgaccaccag ggcggcatcg acatccgcat
     4621 aactcttgga ttctttattc gcccaggatt gggcggtgag ggtacagata cagatcaaat
     4681 acggcgcctg gagcagccat tcacggtggt acagctggtg gaatttttcc gggttggtgg
     4741 cgggtctggc gacgatgatg ccaaatgcct ggcggttgct ggcagtcggc gccatgcgcc
     4801 cggcttcgag gatggcgttt aatttttcag cttccaccgg gttactgcgg tacgcccgca
     4861 cgctgtagcg cctggcaatt aaatcggaga aggtcatgat gtgctcctgc tggctttatt
     4921 ttgatcctat tatacccacc gcccgacttt tctacctgag attataattc gggttggtga
     4981 gataccggtg caagccggta ttctgccaaa aaattaccca ctggaggaac catggctgac
     5041 cgcgtggatg aatttcaagc ctaccgcacc cggatgaatg aacgcatctt tgacctggac
     5101 catctgggga tcaagcgctt tttcaacctg gatacagccg cttaccggga tggcgccctt
     5161 gatccgaaga ccaaagagct gttggggctg gtcgcttcga cggtgctgcg ctgtaatgac
     5221 tgcatcgatt atcacctgat ccagtgccgc gaggccggct ggaaagatga agagctctac
     5281 gatgccctga acgtagccct gatcgtcggc ggcagcatcg tcgttccgca tttacgccac
     5341 gcggtcgaga caatcgatat actacacgaa aaaagcgagt aactgcacga tggaatacct
     5401 gacctttgat gcactccagc agcagctgct cagcctgttt aaagccgggg atttcgaagc
     5461 ggtccgccag ctggctgccg aacagatcgt ccaatacccg gagcagtttc acatcctggc
     5521 ctactggcag atcacagcgg ctgcgcggca aatggagtat cccgccgcac tgcagctgat
     5581 ccggggactg ctggagcgcg gtttctggta cggtgaaagc gtgcttgtaa acagccccag
     5641 cctgcagccg ctgcaagata tgccagaatt catcaacctt ctggaagcca gccgcgccct
     5701 gcgcgcccgc tcggaaaagg atctttaccc cttactgatc ctgcgccagc cagagcgcgg
     5761 cgaaaaaggc ggcccgccca ccccgttgat gatcgccctg cataccaacg g
//
LOCUS       DATMXK010000072         3452 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_43979, whole genome shotgun sequence.
ACCESSION   DATMXK010000072 DATMXK010000000
VERSION     DATMXK010000072.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3452)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3452)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3452
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43979"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2048)
                     /gene="nuoG"
                     /locus_tag="VN363_01380"
     CDS             complement(<1..2048)
                     /gene="nuoG"
                     /locus_tag="VN363_01380"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01973.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA];
                     GO_process: GO:0006120 - mitochondrial electron transport,
                     NADH to ubiquinone [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoG"
                     /protein_id="HWQ83184.1"
                     /translation="MAMLVNLTIDGVQVSVPAGTLIVNAAKQAGIDIPVFCYHPKMEP
                     VGMCRMCLVEIGRPVVDRATGEVQREADGAVKISFGPKLETACTTPVAEGMVVLGASE
                     KAKAGREDILEFLLTSHPLDCPICDKGGECPLQNLTMGFGPGESRYLYDEKMHLAKNL
                     PLGELIYLDRERCIQCGRCVRFQDEIVDDPVIGFYNRGRSLEIVSFSEPGFDSYFSGN
                     TTDICPVGALTTADFRFRARPWELNFAASLCTQCPVGCNTTLNTRREAVSGGKYVVKR
                     VMPRQNEQVNEIWICDKGRFGYHYAQHAERLEQPLIRKNGVLTQASWDEALDLVSSRI
                     RQVDQGLVTLAGGRLTNEDLFNLYQLTTPLQGKAILYTHMAGGDLTAQVGLGTGTNLG
                     DLGRGSAILVVACDLEEEAPIWWLRVKQAADRGATVIVANPRQTKLDRSAKFSLRYPY
                     GSEASVVLAMLNSLSAKRPDLPESARDLLRDEQLKAAAQAFSQAENGIVIFGSEGTSY
                     ESSQALAQACANLLLATDHVGKPNNGLLGVWPRANDQGAWDMGFRPEAELTAALQEAR
                     LLYIVAADPAGDDPGLAGAMQAEFTVVQDLFLTQTGMLADVVLPVQSYAERQGSFTSG
                     ERRVQLYYPAIPERPGSLPDYAVTARIGKRLDIQLEERSASRVMDQIAAGVPAYAGIN
                     "
     gene            complement(2115..3398)
                     /gene="nuoF"
                     /locus_tag="VN363_01385"
     CDS             complement(2115..3398)
                     /gene="nuoF"
                     /locus_tag="VN363_01385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559260.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWQ83185.1"
                     /translation="MAEYILLRHRDVPEIDQLEVYRQHGGFEGFHKAITTMQPQEVTE
                     VVKASGLRGRGGAGFPTGMKWSFIDAKNWPHYVVANADESEPGTFKDREIMERNPFQF
                     LEGIALCAYAIGANAAYIYLRGEFWQLAAKLDTKIAQLHSAGLLGDNLFGTNYSLRIY
                     THLGAGAYICGEETAMLESIEGKLGQPRLRPPFPPSYGLFGKPTVVNNVETLTNVPLI
                     VNNGASWYRTFGTEKSPGTKIFSLSGCINKPGNYELPLGTPFRELIFEHGGGIPDGRQ
                     IKAIMAAGASSSLIVANDTALDTPMDYESVPTVGGALGSASVIVVDESISIPWLINKT
                     MHFFRHESCGKCTPCREGTFWMMHLTDRIQAGQAGMEDVILLQDVANQVKGKCLCALG
                     DFSTEAVTSGIERFRADFEQSTAAEKQPEALAPAD"
BASE COUNT          772 a          996 c          899 g          785 t
ORIGIN      
        1 taattaatcc ctgcgtaggc aggcaccccg gcagcaatct gatccatcac ccgagaggcg
       61 gagcgttctt ctagttgaat atccaggcgc ttaccaatcc gggcagttac ggcatagtcc
      121 ggcaggctgc ccggccgctc agggatcgcc ggataataaa gctgcacccg gcgttcaccg
      181 ctggtgaaac taccctggcg ttcggcatag gattggaccg gcaatacaac atctgccagc
      241 attccagttt gggtgaggaa tagatcttga acaactgtaa attccgcctg catggctcct
      301 gccaggccgg ggtcatcacc agccgggtct gcggcgacga tataaagcag gcgggcttcc
      361 tgcaacgctg cagtgagctc agcttcggga cggaaaccca tatcccaggc gccttgatca
      421 ttcgctctgg gccacacccc gagcaagcca ttattgggtt ttccaacatg atctgtcgcc
      481 agaagcaggt tagcacaggc ttgagccagc gcctggctgg attcatagct ggtgccttca
      541 ctgccaaaga tcacaatacc attttctgcc tgagaaaaag cctgggcggc agcttttagc
      601 tgctcgtccc gcagcaggtc ccgggctgat tcgggcagat cggggcgttt cgccgacagg
      661 ctgttgagca tggccagcac cactgaagct tcgctgccat acgggtagcg taagctgaat
      721 ttggctgagc ggtctaattt ggtctggcgt ggattggcta caattaccgt cgccccccgg
      781 tcggcggcct gctttacccg cagccaccag attggggctt cttcttccag gtcacaggct
      841 accactaaaa tcgcgctgcc tcgacccaga tcgcccaaat tagtacctgt ccccagacca
      901 acttgagccg tcaggtcgcc gcctgccatg tgagtataaa ggattgcttt gccctgcaag
      961 ggtgtggtta gctgatataa attgaacagg tcttcgtttg tcaggcgccc acccgccagg
     1021 gtcacaagac cctggtctac ctggcggatt ctggagctta ccagatcgag cgcttcgtcc
     1081 caggatgcct gggttaatac accatttttt cggatcaaag gttgttcaag gcgttctgca
     1141 tgttgggcgt aatggtaacc aaaccgaccc ttgtcgcaaa tccagatctc attgacctgt
     1201 tcgttctggc ggggcatgac gcgttttacg acatacttgc caccggagac tgcttcacgc
     1261 cgggtattca aggtggtatt gcatccaacc gggcattgtg tacacagaga ggcggcgaaa
     1321 ttcaactccc agggccgggc gcggaagcgg aaatcggcgg ttgtcagcgc ccccaccggg
     1381 cagatatcgg tggtattgcc ggagaagtaa gagtcaaaac caggctcaga aaacgagacg
     1441 atctccaaag accgcccccg gttgtagaaa ccaatcaccg gatcatcaac aatctcatcc
     1501 tggaagcgaa cacaacgccc acactggatg caccgctcgc gatccagata aatcaattcg
     1561 ccaagaggga ggtttttggc caggtgcatc ttttcatcat agaggtagcg cgattcacca
     1621 ggaccgaatc ccatggtcag gttctgcaga gggcactctc cgcctttatc acaaatggga
     1681 caatccagcg ggtgcgaggt taacaagaat tccaggatgt cttcacgccc ggctttcgcc
     1741 ttttcgctgg ctcctaatac caccatgcct tcggcgacgg gtgttgtgca ggctgtttcc
     1801 agcttagggc caaagctaat tttcaccgcg ccatccgctt cgcgctgcac ttcaccggtg
     1861 gcgcgatcca caaccggcct tccgatctct accaggcaca tccgacacat accaaccggt
     1921 tccattttgg ggtgatagca gaagaccggt atgtcaatgc cggcttgctt ggcggcattg
     1981 acaatcagcg taccagccgg gaccgaaacc tgaacaccgt caattgtcag atttaccaac
     2041 attgccatgg actactccgt gatcacaaag cgggccagct tgagcggcag agcgctttgc
     2101 agattcgcat cttatcagtc tgccggtgca agcgcttccg gctgtttttc cgcagctgta
     2161 ctttgctcaa aatcagcccg gaaccgttca atccctgaag tgactgcttc cgtagaaaag
     2221 tcacccagtg cgcacagaca ttttcctttg acctggttgg cgacatcttg caggaggatc
     2281 acatcctcca tgcctgcctg tccggcctgg atgcgatcag tcagatgcat catccagaaa
     2341 gtgccttccc ggcacggggt gcattttcca cacgattcat ggcggaagaa atgcatggtt
     2401 ttattgatca gccagggaat actgatcgat tcatccacga caatcaccga ggctgaacct
     2461 aatgcaccgc ccactgttgg cacactttcg taatccattg gggtgtccag ggctgtgtcg
     2521 ttggccacga ttagcgacga ggaagcccca gccgccatga tggctttaat ctggcggcca
     2581 tcagggatcc ctccgccatg ctcgaagatc agctcccgaa aaggggtgcc caggggcagc
     2641 tcataattac cgggcttatt gatgcagcct gacaacgaaa aaatcttggt gcccggactc
     2701 ttctcggtgc caaaggtgcg ataccagctg gccccattgt ttacgatcaa tggcacgttg
     2761 gtcaatgtct ctacattatt tacaaccgtg ggtttaccaa ataggccata gctgggcggg
     2821 aaaggcgggc gcaatcgagg ctggcccagc ttcccttcaa tcgattccag catagcagtt
     2881 tcttcaccgc agatatacgc gccggctccc aggtgagtat agatgcgtaa cgaataatta
     2941 gtgccaaata gattatcccc cagcaggcct gccgaatgca gctgcgctat tttggtatct
     3001 agtttggctg ccagctgcca gaattcgccg cgcaggtaga tataagccgc gttcgcgccg
     3061 atcgcatatg cacataaagc gatgccttcc aggaactgga agggattgcg ctccatgatc
     3121 tcgcgatcct taaatgtacc cggctccgat tcatccgcat tggctacgac gtagtgcggc
     3181 cagttcttag catcgatgaa tgaccacttc attccggttg gaaagcctgc tccaccccgt
     3241 ccccgcaaac ccgatgcttt gacgacttcg gtaacctcct ggggctgcat ggttgtgatc
     3301 gctttatgaa atccttcaaa accgccatgc tggcgataga cttctagctg atcgatctcg
     3361 ggtacatccc ggtgacgaag aaggatatat tcagccataa tgctatttct ttccttcccg
     3421 cgcccgcagt ccattcacga attcgagagc gc
//
LOCUS       DATMXK010000073         4652 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_65199, whole genome shotgun sequence.
ACCESSION   DATMXK010000073 DATMXK010000000
VERSION     DATMXK010000073.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4652)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4652)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4652
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65199"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            8..316
                     /gene="nuoK"
                     /locus_tag="VN363_01390"
     CDS             8..316
                     /gene="nuoK"
                     /locus_tag="VN363_01390"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002638444.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoK"
                     /protein_id="HWQ83186.1"
                     /translation="MIPISWYLVFAAALFSIGLFGVLARKNAIAILMGIELMLNSVNV
                     NLVAFWRYNTPEMITGQVFAIIVFAVAAAEVAVGLALLISVYRRRKTVVADEINLLKW
                     "
     gene            320..2509
                     /gene="nuoL"
                     /locus_tag="VN363_01395"
     CDS             320..2509
                     /gene="nuoL"
                     /locus_tag="VN363_01395"
                     /EC_number="7.1.1.2"
                     /inference="COORDINATES: protein motif:HMM:NF005141.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit L"
                     /protein_id="HWQ83187.1"
                     /translation="MASETLIWLIPVPPLLAFFLILLFTNRSNRLSHIVGVGAALLSF
                     LGSMVVFFRAISTEHLGEHPFASSVNWLATGEKYLTVGVLIDPLSAATLFFVSITILM
                     IFLYSVGYHNFGQPKGDHDQPGLPPHGAAVTDAHGHSHKVPSIEPMYARFFAFIALFA
                     FGMYLLVVSDNLVTLFAGWEVMGLCSYLLIGFWYAKESARAAMVKAFLTTRIGDMFML
                     LGIGYLYSITGTLNFRDILYNEEVLHMLATTPAVGGFLGLSAAGLIGILLFMGTVGKS
                     AQFPLHVWLPDAMEGPTPVSAMIHAATMVSAGVYMVIRMFPLISAGMEHGELTSAMTV
                     MTVIGAFTALFAATIALAQNDIKRVLAYSTISQLGYMIAALGIGAYVAAAFHLITHAF
                     FKALLFLGSGSVIHGMEHGVLHTGEHVDPQDMYNMGGLRKKMPVTFWTFLIGGFALSG
                     FPLVTAGFWSKDEILADAFGHGHYVVFGVLALAALLTAFYTMRQITLTFLGEPRTKAA
                     EHAQETPWTMTLPLSILAVFAIGAGWVGIPEHFPLLGGLIPNWFHEFVGGTLLEHPTA
                     VDFNIVPLLTSLVVALGGLWAGWTVYSRVKTTDAIDPLKTALGGMYKYLKNKYYVDEI
                     YELVFIRPAQWMSATFTSLWMDRTVIDGFLHGVAASTLKIGSVLRNYFDAPVVNGFGD
                     IVGEGAKKLGQVLRPIQTGRIQQYMIVALVSVAAFSALFYYLLVYTR"
     gene            2533..4080
                     /locus_tag="VN363_01400"
     CDS             2533..4080
                     /locus_tag="VN363_01400"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWQ83188.1"
                     /translation="MAFLENYLLSFILFTPVAAAVLLLFVPRDNKNLIRWVAFIASFI
                     PLILTLVTWFNFDSNLAGFQFEEKFVWYGALNSSYHLGIDGISLSMVLLTTLLTPLVI
                     LASFNIQDNVRTYMMLFFLLEFGMLGVFLTLDLLLFFVFWEFGLVPMYFLINQWGSEK
                     GERQIWGGIKIPARTYASFKFMIYTMAGSLGLLLAIQMIGVVSGTFDLVELFQRWPTV
                     DGTLFGLPAETVKAIAFWAFAVAFAIKVPVWPFHTWLPDAHTEAPTAGSMILAGVLLK
                     LGAYGFIRLVLPLYPAESSQYAPVLAFLAVMGIIFGALAAYGQTDFKRLVAYSSVNHM
                     GFVVLGIAAAAAATGTENANIALNGAVLQMFNHGLSAAGMFFLVGVIYERTHTRDLNE
                     YGGLFPLVPVYGGILIFTSMASLGLPGLNGFVSEFLVVRGSWPVLPLYTALGMIGLFF
                     TGAYILKGIGKVLHGPLNQNWVGHLTEISPREIFVIAPLMILILWIGIFPGWILDVIN
                     RAVALFF"
     gene            4151..>4652
                     /locus_tag="VN363_01405"
     CDS             4151..>4652
                     /locus_tag="VN363_01405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83189.1"
                     /translation="MQFPILSAIVFLPIITALILLMIPGERKTEIRVTALAAAIFALV
                     LSGWVYFSYDIAQGGYQFIEKYDWLPALGISFHVGVDGMNAPLVLLTGVVMFTGVLIS
                     WKIDDRPREFFAFLFILATGVFGVFVTLDLFMLFFFYEIAVFPMYLLIAIWGWKQTRE
                     YAAMKLT"
BASE COUNT          865 a         1219 c         1235 g         1333 t
ORIGIN      
        1 tttataaatg atcccaatat cctggtacct ggtttttgct gccgcgctct tcagcatcgg
       61 gctgtttggc gttctggcgc gtaaaaacgc gatcgcgatc ttgatgggca ttgagctgat
      121 gttaaattcg gtcaatgtca acctggtggc attttggcgc tacaacacgc cggaaatgat
      181 caccgggcag gtatttgcaa tcattgtttt tgctgtggca gccgcagaag tcgcggttgg
      241 cctggctctg ttgatatccg tctatcgccg ccggaagaca gtcgtggcgg atgagatcaa
      301 cctgttgaaa tggtgaccta tggcatctga aactctcatc tggctaattc ctgtaccccc
      361 gctgctggca tttttcctga tcctgctttt cacaaatcgc agcaaccggc tcagccacat
      421 cgtcggtgtt ggcgcagcgt tgctctcgtt cttaggcagc atggttgttt tcttccgggc
      481 gatatccacg gagcacctcg gtgaacatcc ttttgcttcc agtgtgaact ggctggcgac
      541 gggcgagaaa tacctcacag taggtgtctt gatcgatcca ctctcggctg caacgctgtt
      601 tttcgtctcg atcaccatct tgatgatctt cctgtacagc gtgggatacc ataattttgg
      661 ccagccgaaa ggtgatcacg accagcccgg cttacctccc catggcgctg ccgtcaccga
      721 tgcccacggg cattcgcaca aggtgccctc catcgagccg atgtatgccc gattctttgc
      781 gtttattgcc ttattcgctt tcggcatgta cttgctggta gtaagcgata acctggttac
      841 tctgttcgcc ggctgggaag tgatgggcct gtgttcatat ctgttgattg gcttctggta
      901 tgccaaagag tctgcccggg ctgccatggt aaaagccttt ctcaccaccc ggatcgggga
      961 tatgttcatg ctgctgggca taggctacct ctattcaatc accggcactc tgaatttccg
     1021 ggacatcctc tacaacgagg aagttctcca catgctggca accacgccgg cagtaggggg
     1081 atttttgggg ctgtctgccg ccggcttgat cggcattctc ctcttcatgg gcacggtcgg
     1141 taaatctgct caattcccat tgcatgtctg gctgccagat gcgatggaag gtccgacccc
     1201 ggtttcagcc atgatccatg ctgcaaccat ggtttcagcc ggcgtttaca tggtcatccg
     1261 catgttccca ttgatctctg caggtatgga gcacggcgag ttgacctcgg cgatgaccgt
     1321 gatgaccgtt atcggggcgt ttacagccct ctttgcagcc accattgccc tggcacagaa
     1381 tgatattaaa cgggttctgg cttactcaac aatctcacag ttgggctata tgattgctgc
     1441 tttggggatc ggtgcgtatg tcgcagcggc tttccacctg atcacccatg cattcttcaa
     1501 ggcactcctt ttcctcggct caggttcggt aatccatggg atggagcacg gtgttctgca
     1561 taccggcgag catgtagatc cccaggatat gtacaatatg ggcggcctgc gcaagaagat
     1621 gccggtcacc ttctggactt tcctgatcgg cggttttgct ctttctggat tcccactggt
     1681 cacggctggc ttctggtcca aggatgagat cctggcagat gccttcgggc atgggcatta
     1741 cgtggtcttt ggggtactcg ccctggcagc cctgctgacc gccttctata ccatgcgcca
     1801 gatcaccctg actttcctgg gagagccacg caccaaagcc gcagagcatg ctcaggagac
     1861 tccctggacg atgaccctgc ccttgagcat cctggcagtc tttgccatcg gcgccggctg
     1921 ggtgggcatc ccggagcact ttcccctcct cggaggtctc attccgaact ggttccacga
     1981 atttgtcggc gggacgcttc tggaacatcc caccgctgtt gattttaaca tcgtgccatt
     2041 gctgacctct ctggttgtcg ctttaggcgg cttatgggct ggctggacgg tttacagccg
     2101 ggtgaaaaca acggatgcaa ttgatccgct gaaaaccgct ttggggggca tgtacaagta
     2161 cttgaaaaac aaatattacg tcgatgaaat ttacgagctg gtatttatcc gcccggctca
     2221 atggatgagt gccacattta cttctttgtg gatggatcgc acggtcatcg atggtttctt
     2281 gcacggcgtt gctgcctcaa ctttgaagat cggctctgtt ttacggaatt attttgacgc
     2341 cccggtggtt aatggtttcg gcgatatcgt cggcgaggga gccaaaaagc tgggtcaggt
     2401 tctgcgtccc atccaaaccg gtcgcatcca gcaatacatg atcgtggctc tggtgagcgt
     2461 tgctgcattc agcgccctgt tttattactt gctggtttat acccgctaag cgaccgacag
     2521 taggagcgac acatggcgtt cttagaaaat tatctgcttt ctttcatctt attcacacct
     2581 gtcgcggcgg cagtcctttt gctgttcgtg ccccgcgata ataagaacct gatccgttgg
     2641 gttgctttca ttgccagttt tatcccattg atcctgactc tggtgacctg gtttaatttt
     2701 gacagcaatc tggcaggttt ccagtttgaa gaaaaatttg tctggtacgg cgccctgaat
     2761 tcctcctatc atctgggaat tgatggtatt tcgttgagca tggtcttgct caccacttta
     2821 ctcactccgc ttgtgatcct ggcttccttc aatatccagg ataatgtacg gacttacatg
     2881 atgctcttct tcctgctgga atttgggatg ttgggtgtct tcctgaccct ggacttgttg
     2941 ttgttctttg tcttctggga atttggcttg gtcccgatgt atttcttgat taaccaatgg
     3001 gggagcgaaa aaggcgagcg ccagatttgg ggtgggatca aaattcccgc tcggacctat
     3061 gcttctttca agttcatgat ctacaccatg gctggttccc tgggtctgct gttggcgatc
     3121 cagatgatag gtgtggtttc ggggactttt gatctggttg agcttttcca gcgctggcca
     3181 acggtggatg gcaccctgtt cggtttaccg gctgagacgg tgaaggcaat cgcattctgg
     3241 gcttttgccg ttgcttttgc gatcaaagtc ccggtctggc cgttccacac ctggctgcca
     3301 gatgcacata ccgaagcccc cactgccggc tcgatgatcc tggcaggtgt tttgctgaaa
     3361 ttaggcgcct acggttttat ccgccttgtc ttgcccctct atccggctga atcgagtcaa
     3421 tatgctccgg ttctggcctt cctggcggta atggggatca tttttggtgc cctggcagcg
     3481 tatgggcaaa cggatttcaa acgcctggta gcttactcct cggtcaacca tatgggtttt
     3541 gttgtgctgg gtattgccgc ggcagccgcg gcgacaggta cagaaaacgc aaatatagcc
     3601 ctgaacggcg cagttttaca gatgttcaac cacgggcttt cggcggctgg aatgttcttc
     3661 ctggtggggg tgatctatga gcgtacgcac acccgcgact tgaatgaata tggcggatta
     3721 ttcccactgg ttccggtgta tggcggcatc ctgatcttca cttccatggc atcactgggt
     3781 ctccccggtc tgaatggctt cgtctccgaa ttcttggttg tccgaggctc ctggccagtc
     3841 ctgcctctct atactgccct cggcatgatc gggctgtttt tcacgggcgc ctatattttg
     3901 aaagggatcg gcaaagtact ccacggtccc ctgaaccaga attgggtggg tcatctcact
     3961 gaaatcagcc cgcgcgagat ctttgtaatt gcgcctctga tgatcctgat cttgtggatc
     4021 gggatctttc ccggctggat cttagacgtt atcaaccgcg cggtcgcgtt gttcttctag
     4081 gcatggtcgt tattatgact ggcttatata cagtaagacc tgcatcacgc actcgtgcat
     4141 gaggtgaaga atgcaatttc caattcttag tgctatcgtt tttctaccga tcatcacggc
     4201 cctgattctg ctgatgatcc cgggtgagcg caaaaccgaa atccgggtca cagccctggc
     4261 agctgcgatc ttcgccctgg ttttatctgg ctgggtgtat ttttcctacg acatcgctca
     4321 gggtggttac cagttcatcg aaaaatatga ttggctgcct gccttaggga tatccttcca
     4381 tgtgggggtg gatgggatga atgctcctct ggtcctgttg accggggtgg ttatgttcac
     4441 cggggtgctg atctcctgga agatcgatga ccggccgcgg gaattcttcg cctttttatt
     4501 tatcctggca accggagtgt tcggcgtttt cgtcacgctc gacttgttca tgttgttctt
     4561 tttctacgaa attgccgttt tccccatgta cttgctgatc gcaatctggg gttggaagca
     4621 aacccgagaa tatgcggcca tgaagctaac cc
//
LOCUS       DATMXK010000074         2174 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_43825, whole genome shotgun sequence.
ACCESSION   DATMXK010000074 DATMXK010000000
VERSION     DATMXK010000074.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2174)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2174)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2174
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43825"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..411
                     /locus_tag="VN363_01410"
     CDS             <1..411
                     /locus_tag="VN363_01410"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWQ83190.1"
                     /translation="YRRVLLPQIRAEAELAHEHGAKFGYICSSGTKPMLDDYLEAGID
                     VLIGVDPIQGTHTNLPLMKEKLNHRVCLWGGVSGAITVEQGSEVEVRSAVREAQQTLG
                     SNGFILSPVDNLTVDEPRTWTNVKILIDEWNRVR"
     gene            complement(408..1379)
                     /locus_tag="VN363_01415"
     CDS             complement(408..1379)
                     /locus_tag="VN363_01415"
                     /inference="COORDINATES: protein motif:HMM:NF009498.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tagatose 1,6-diphosphate aldolase"
                     /protein_id="HWQ83191.1"
                     /translation="MSLSTGKRRGLAQCSTPRGTFAILALDHRNNLRRAINPTDPNAV
                     TYEIMAEFKQDVVQALAPSCSAVLLDPEIGSARVVAQDALPGNVGLLVAVEATGYSSE
                     PTDRLSEILAGWSVAKIKRMGASGVKLLIYYHPNARHAHDQENLVSQVADDCVKYDLP
                     LFLEPLSFSLDPQKKLSSAELRYVVIETARRLSPLGIDILKAEFPINVAVVPDQEVWL
                     EACLELGRASSVPWALLSAGVDYETYLQQVTIACQAGASGVLAGRAVWKEAIDYQGWE
                     RQRFLSTIATERMARLWGLCDALAKPWKEAYPIGGAGETWYQEYPEY"
     gene            complement(1379..>2174)
                     /locus_tag="VN363_01420"
     CDS             complement(1379..>2174)
                     /locus_tag="VN363_01420"
                     /inference="COORDINATES: protein motif:HMM:NF019625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class II D-tagatose-bisphosphate aldolase,
                     non-catalytic subunit"
                     /protein_id="HWQ83192.1"
                     /translation="PGGAQGAMHKDWITRPEQVQETMDLTHTAFLERGLEAAWERVIA
                     IVVQPGVEFGDQEVIDYHPDLSRSLSLFIESLDHLVYEAHSTDYQLPSGLRQLVADHF
                     AILKVGPALTFALREAVFALAQIEEESLAGRSGTQLSQVRTILENAMQSNPKDWLKYY
                     RGDPHEVAIARKYSYSDRIRYYWSTPTVRSALTQLLHNLETEPVPGSLISQYLPQQYW
                     KMRNGELDRSPRAIIQDKIQEVLQDYAYACGYRAAPSTRLSSGRSR"
BASE COUNT          545 a          588 c          535 g          506 t
ORIGIN      
        1 tatcgccggg tgctgctccc tcaaatccga gccgaggcag aattggctca cgaacatggc
       61 gcaaaattcg ggtatatctg ttcgagcggg acaaaaccga tgcttgatga ttatctggaa
      121 gccggcatcg atgtattgat cggggtcgat cctatccagg gaacgcacac gaatttaccc
      181 ttgatgaaag aaaagctgaa tcacagagtc tgtctttggg gaggcgtgtc gggagcgatc
      241 actgtcgagc aaggaagtga ggtagaagtg cgtagtgctg tgcgggaggc ccagcaaacc
      301 ctgggatcga atgggtttat cctttccccg gtagacaatt taaccgtcga cgagcccagg
      361 acctggacaa acgtcaaaat tctgatcgac gaatggaatc gcgtccgtta atattccggg
      421 tattcctggt accaggtttc acccgctcca ccgattggat aagcctcttt ccacggtttc
      481 gccagagcat cacataaacc ccacaaacgc gccatacgct cagtcgcaat cgtgcttaaa
      541 aagcgctggc gctcccatcc ctgataatct atcgcctctt tccacaccgc ccgcccggcc
      601 agaactccag acgcccctgc ctgacacgca atcgttacct gctgtaaata cgtctcataa
      661 tccactcctg cagacagtag cgcccagggt acggatgagg cgcgaccaag ctcaaggcaa
      721 gcctccagcc atacttcctg gtctggcacg actgccacgt tgatcgggaa ttctgctttg
      781 agaatatcga tccccagcgg agaaagacgc cgggcagtct cgataaccac ataacgcagt
      841 tccgcactcg aaagcttctt ctgagggtct aatgaaaatg aaagcggctc caggaatagg
      901 ggaagatcgt atttgacaca gtcgtccgcg acctggctaa ctaaattctc ttgatcatgg
      961 gcatgccggg catttggatg ataataaatc aatagtttta cgccgctcgc gcccatgcgc
     1021 ttgatcttgg caacgctcca accagctaag atctcactta accggtcagt aggttctgac
     1081 gaatatccag tcgcttctac tgcgaccagc aaaccaacat ttccgggcaa cgcatcctga
     1141 gcaaccactc gcgccgagcc aatttctgga tccaataaga ccgcgctgca gcttggtgca
     1201 agtgcttgga caacatcctg tttgaattcc gccatgatct catacgtaac agcatttggg
     1261 tcagttgggt tgatagcccg gcgcaagtta ttccggtgat ctaaggcaag gatagcaaaa
     1321 gtaccccgtg gcgttgaaca ttgcgccaat cctctacgtt ttccagtact cagcgacatt
     1381 acctactcct tccagaactt aaccgcgtgc tgggagcagc gcgatatccg caagcataag
     1441 cataatcctg cagtacttcc tggattttat cctgaatgat cgcccttgga gagcgatcca
     1501 gctctccgtt gcgcattttc caatattgct ggggcagata ctggctgatc agcgaaccag
     1561 ggacgggttc tgtttccaga ttgtggagta actgggttaa cgccgatcga acagtagggg
     1621 tggaccaata ataacggata cgatcactat agctgtactt tcgggctata gcaacttcat
     1681 gagggtcgcc ccggtaatat ttcaaccaat cctttggatt ggattgcatc gcattttcca
     1741 ggattgttcg cacctgagat agctgggtcc cagaacgacc tgccagactc tcttcttcaa
     1801 tctgggcaag ggcaaaaaca gcctcgcgca gggcaaaggt aagcgccggt cctactttta
     1861 ggatagcaaa atgatcagct actaattggc gcaatccaga gggcagctgg taatcggttg
     1921 aatgagcctc ataaaccaga tgatccaatg actcgatgaa taggcttaaa gaccggctca
     1981 aatcagggtg ataatcaatg acttcctgat ctccaaattc aactccaggt tgcacaacga
     2041 tggcgatgac gcgctcccag gcagcttcta atccgcgctc gaggaaagcg gtatgcgtta
     2101 gatccatggt ttcttgtact tgctctggcc gggtaatcca atctttatgc attgcccctt
     2161 gagcgccccc aggc
//
LOCUS       DATMXK010000075         5604 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_133604, whole genome shotgun sequence.
ACCESSION   DATMXK010000075 DATMXK010000000
VERSION     DATMXK010000075.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5604)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5604)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5604
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133604"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            136..1224
                     /gene="mobB"
                     /locus_tag="VN363_01425"
     CDS             136..1224
                     /gene="mobB"
                     /locus_tag="VN363_01425"
                     /inference="COORDINATES: protein motif:HMM:TIGR00176.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-guanine dinucleotide biosynthesis
                     protein B"
                     /protein_id="HWQ83193.1"
                     /translation="MESLAELLSRSAARHHKLCPRQVLGVRMGMLAGKVLNLELPQQD
                     KRLFTFVESDGCGMGGIAVATGCFAERRTMRVLDYGKLAATFVDTQTGAAFRIHPHPE
                     GRTRARAHFPEYNRWQSQLEGYQVLPDEDLFIVEPVQLNVSVEKIISQPHLKVTCAQC
                     GEEINNAREVIRDGQSLCRSCAGEGYYTTAILTTQPAKSSIPVVTVIGKSGSGKTTLL
                     EKLIKELSIRGYRLATIKHHSHSGFDIDVPGKDSWRFARAGSCHVVIAAPDKWAAYRH
                     LQADLSLDEITAPIQDVDLILVEGFKQANKPTIEVVRSANSQTLIGTPEQRIAVVSDM
                     QLEVGVPLYALDDVQGLAAFIENRFLTT"
     gene            1300..2349
                     /locus_tag="VN363_01430"
     CDS             1300..2349
                     /locus_tag="VN363_01430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83194.1"
                     /translation="MSASLFPGLLLPDHYDSGKAGQIWKVSYENTAAAAREWAARYSI
                     PPAAADGFRIALLMVDVQNTFCLPDFELYVGGRSGTGAVEDNRRLSAFIYRNLGRLTE
                     ITLTLDTHQAMQIFHAIYLIDADGKHPTPYTLVSAADILEGRWRFNPSVAHSLDISPE
                     YGQEQLADYVQQLDARQKYALTIWPYHSMLGGIGHALVPLIEEAVFFHTIARAAQADF
                     ILKGANPFTESYSAIGPEVLEDPAGKQLAKKNDQIVRLVEQFDAVVVAGQAKSHCVAW
                     TIGDLLSDIQARDPALANKIYLLDDCSTPVVIPGVIDYTDQAEAAYQRFTEAGMHRVL
                     STDLMENWPGMPPVS"
     gene            2740..3522
                     /locus_tag="VN363_01435"
     CDS             2740..3522
                     /locus_tag="VN363_01435"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWQ83195.1"
                     /translation="METEALAAPENTENPELENIKRKMQFSGTVIKTTIAGAVVDIGL
                     STPGVVHISQLSTDPVNRVEDVVTTGQQVEVWVKRVFPKKNRVELTMIRPLELEWREI
                     VKGMNVKGKVTRLEKFGVFVDVGAERPGLVHISEMTHGYIKSPGEMVKEGDEIEVQVL
                     NVNRQKKQIKLSMKALEEKPEKVVKEVQAQPSRPAVQAEEAPKEEAIPTAMEMALREA
                     MERSKSREPAAPVSRSKQKAPAANNEIEQILARTLKNKAKTR"
     gene            complement(3619..4941)
                     /locus_tag="VN363_01440"
     CDS             complement(3619..4941)
                     /locus_tag="VN363_01440"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83196.1"
                     /translation="MGFFDLLTLIIDNLGRRKGRVALTAIGVIIGTAAVVVLVSLAIG
                     LQKNANEQLTGIGDLTQIQVYPGYKEQGPGMMMVAVGPGQPDPNVTLITDDTLAQITA
                     MAGVTEVIPRDYLMAGGSIKIDRLETYPNFIGIDVNDLSTLGIQAAEGTLELSKGNIV
                     VGAMVKNQFYDPRWRPGQTMPEPPELMGKTLRLTLSKYSQEGTETRKVIPLTVVGTTA
                     ESRGESDYSIYLPLDDLVAMNEWALGKRINRNRDGYNMLIVKVDNLDTVLEVTDQITA
                     LGLQAYTPQSFVQGINSFYLVLQVIFGGVGAIALLVAAIGIANTMAMAILERTREIGL
                     MKAIGATNRDVLSVFLGEAAGIGFLGGLGGVIIGWSAGQVINVLAMAYLAGQAVETGG
                     APPSVAVFTPTWLPIFTLVFATIIGLVSGLYPALRAATLIPVEALKYE"
     gene            complement(4965..>5604)
                     /locus_tag="VN363_01445"
     CDS             complement(4965..>5604)
                     /locus_tag="VN363_01445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83197.1"
                     /translation="ITLLVFSVPQLEVSFYRDAGQFFVGQPNQLPLQVVNLGRKQAVL
                     GSMRVVAQPAEGMPGAQMMNNSTLVGALDPGGYYTLDTTMIPEAPGPLDLLITLDYTD
                     DFNQAQVITRTISIVVEEMMVGPEGGIPPDGGFPQNGGEFPGGEIPGGEFPTDGGAQT
                     ETFGQKVVRFLKGLFGLDSAQPSGGGEIMPPVEGIPEEGPSEAVPVAPPIKG"
BASE COUNT         1285 a         1590 c         1518 g         1211 t
ORIGIN      
        1 gaagcgcggg cgttcttacc cgagcacgcc cggagtatgc cgatcaaaat cggaaagctg
       61 gattggttga tcctgactct gggtgtgatc ggcctgctgg ctttcgcgct catccctgtt
      121 taggagaacg ccatcgtgga atctctagct gaattattat cccgctcagc tgcccgccac
      181 cacaagctct gcccgcgcca ggtacttggc gtgcgcatgg gtatgctggc aggaaaagta
      241 ttgaaccttg agctccctca acaggacaaa cgcctgttta ctttcgtgga gtctgacggc
      301 tgtgggatgg gcgggatcgc ggtcgctaca ggctgcttcg ccgaacggcg gacgatgcgc
      361 gtactggatt atggcaagct ggcggcaacc ttcgtcgata cccagaccgg cgcggctttt
      421 cggatccacc cccatcccga agggcgcacc cgggcccggg cacattttcc cgaatacaat
      481 cgctggcaaa gccagctcga aggttaccag gtgctgccag atgaggatct tttcatcgtc
      541 gagccggtcc aactcaacgt atcggtagag aaaatcatca gtcagcctca tctaaaagtg
      601 acctgcgccc agtgcggcga agaaatcaac aatgcgcggg aagtgatccg ggatggccag
      661 agcttatgcc gttcctgcgc gggcgaaggt tattacacca cggccattct cacaacccag
      721 ccggcaaaat cctccatccc ggtggtgacc gtgatcggta aatccggatc tggcaagaca
      781 accttgctgg aaaaattgat caaggaacta agtattcggg gctaccggct ggcgacgatc
      841 aaacaccatt cgcacagcgg gtttgacatt gatgtccccg gcaaagacag ctggcgattt
      901 gcccgggcgg gcagctgcca tgttgtcatt gccgctccgg ataaatgggc ggcataccgc
      961 catctccagg ctgacctcag cctggacgaa atcaccgccc cgatccagga tgtggatttg
     1021 atcctggtcg agggcttcaa gcaggccaat aaaccgacca tcgaagtcgt gcggtctgcg
     1081 aacagccaga ccttgatcgg gacacctgaa cagcgcatcg cggttgtctc cgatatgcag
     1141 cttgaggtgg gggtacccct gtatgcgctg gatgatgtcc agggcctggc tgcattcatt
     1201 gaaaaccgct tcctgacgac gtgagcaaac ctacccatcg actaattgtt tcatagtata
     1261 actatgagac ggttattctt gatttaagaa ggagttctga tgtctgcatc tttattccct
     1321 ggtctgctgc tgcccgatca ttacgattct gggaaagccg gtcagatctg gaaagtatcc
     1381 tatgaaaata ccgccgcggc tgctcgcgaa tgggctgccc gttacagcat ccccccggct
     1441 gcggctgacg gctttcggat tgctctcttg atggtggatg tacagaatac attttgcctg
     1501 cccgatttcg agttgtatgt cggtgggcgc tcgggtactg gcgcggtcga agataaccgc
     1561 cgcctatcgg cttttatcta tcgcaacctc gggcgtctga ccgaaattac gctcaccctg
     1621 gacacccacc aggcaatgca aatattccat gccatttacc tgatcgacgc cgatggcaag
     1681 catcctaccc cgtataccct ggtcagtgca gccgatatcc tcgaagggcg ctggcgcttc
     1741 aatccatctg ttgcccacag cctggatatc agcccggaat acgggcaaga gcagctggcc
     1801 gattacgtcc agcagctgga cgcgcgccag aaatatgccc tgacgatctg gccttatcat
     1861 tccatgctgg gtgggattgg gcacgccctg gtgccgctga tcgaggaagc ggtattcttc
     1921 catacaatcg cccgcgccgc tcaggccgat ttcatcctca agggtgccaa tcctttcacg
     1981 gaaagttatt cagccatcgg gccagaagtg ctggaggatc ccgccggcaa gcagctggcg
     2041 aaaaagaatg atcagattgt ccgcctggtg gagcagtttg acgctgtggt ggtcgccggg
     2101 caggccaaga gccactgcgt tgcctggacc atcggtgact tgctaagcga tatccaggct
     2161 cgcgatcctg ccctggcgaa caagatctac ctactggatg actgcagcac acccgtagtt
     2221 attcctggag ttatcgacta caccgatcaa gccgaggctg cttaccagcg ctttacagaa
     2281 gccgggatgc accgggtgct gtccaccgat ctgatggaaa attggcccgg gatgcctccg
     2341 gtatcctgac ggtcgaaatg cgacaaattc tatctccatt gacaatcggg tggaattgtg
     2401 tgatactaca ctcctcggag ttgcccgaaa ggtaacccgc cctgatttcg ccatcttaat
     2461 caggaggagg tgttgctaat atgtctgacc atagtaccaa acttagcgca gtggcaaccc
     2521 tcctcgtaat ccgataggtt gccgctgcgc gacagggagc cgtctgagtt attcagacgg
     2581 cttcttgttt ttcattcgca cattcaattc tcaagtttga cttaacaggt ttggagcggt
     2641 ataatcactc ttcgcgggtg taagcctccg cggcttaagg atttaagcat atccgccgat
     2701 ttgggtggat cgaaattagt ccgattggag taatgtgcta tggaaacaga agccctggca
     2761 gccccagaaa acactgaaaa ccccgaactt gagaatatca aaaggaaaat gcagttttcg
     2821 ggaaccgtga tcaaaacgac tatcgccggt gccgtcgtag acatcggtct cagcacccct
     2881 ggtgtagtgc atatttcaca gctttcgacc gatccggtca accgcgttga agatgttgtt
     2941 accactggac agcaggtaga ggtatgggtt aaacgggtat ttcccaagaa aaaccgggtt
     3001 gaattgacga tgatcaggcc gctcgagctg gagtggcgcg aaattgtcaa agggatgaat
     3061 gtcaaaggca aagtaacccg tttggagaag tttggcgtct tcgtggatgt tggagccgag
     3121 cgtcccggcc tggtgcatat cagcgagatg acccacggct acattaagag cccgggcgag
     3181 atggtaaaag aaggcgatga gatcgaggtt caggtgctga acgtgaatcg ccagaaaaag
     3241 cagatcaagc tcagcatgaa ggccctcgaa gaaaaaccgg agaaagtggt caaggaagtt
     3301 caggcccagc cttcaaggcc tgccgttcag gctgaggaag cccccaagga agaagccatc
     3361 ccaactgcca tggaaatggc cctgcgcgag gcaatggaac gctccaagag tcgtgagcct
     3421 gccgcgccgg tcagccgcag caagcaaaag gcccccgctg ccaataatga gatcgaacag
     3481 atcctcgcgc gtacgctgaa gaataaagcg aagacccggt aagctcaatt ttctggaact
     3541 gcaggacctc gttgttaaac acaactcggc tcaactgcta aactggttga gccgaatata
     3601 tttaatcgca ccgctgcgct actcgtactt cagcgcttcg accggaatca gtgtggctgc
     3661 ccgcaaagcc gggtacaaac cactgaccag gccgataatt gtggcgaata ccagggtgaa
     3721 aattggcagc caggtaggcg tgaataccgc aactgagggt ggagcgccgc ctgtctcaac
     3781 cgcttgccct gccagatagg ccatcgccag cacgttgatc acctgcccgg cgctccagcc
     3841 aatgatgact ccgcccagcc ctcccagaaa gccaattcca gccgcttcac cgagaaagac
     3901 gctcaagaca tcccggttgg tcgcgccgat ggctttcatc agtccgatct cccgagtgcg
     3961 ttccaggatt gccatggcca tggtattggc tatgccaatc gccgcaacca gcaaagcgat
     4021 ggcgcctacg ccgccaaaga tcacctgcag aaccagatag aagctgttaa tcccctgaac
     4081 gaacgattgt ggcgtatatg cctgcagccc caaagctgta atctggtctg tgacctccaa
     4141 tactgtgtca aggttatcca ccttgacaat caacatatta tagccatccc ggttgcggtt
     4201 gatgcgcttg ccaagagccc attcgttcat cgctaccaga tcatcgagag gcaggtagat
     4261 tgagtaatcc gactcacccc gggattcagc ggtggtccct accacagtca gtgggatgac
     4321 cttgcgtgtt tctgtccctt cctgactgta tttcgacagg gtcaggcgta aggttttacc
     4381 catcaattca ggtggctcag gcatcgtctg cccgggacgc caacgcgggt cataaaactg
     4441 atttttgacc atcgccccga ctacaatatt acccttggag agctcaaggg tgccctcagc
     4501 agcctggatt cccagcgtac tgagatcgtt gacatcgatg ccaatgaaat tgggataggt
     4561 ttccagacga tcgatcttga tcgaaccgcc tgccatcaaa taatcgcggg ggatcacctc
     4621 ggttacccct gccatcgcgg taatttgagc cagagtgtcg tcggtgatca gagtgacgtt
     4681 gggatcaggc tgcccgggac ccaccgcgac catcatcatc ccgggtcctt gttctttgta
     4741 accaggataa acctggatct gggtcagatc gccgatcccg gtaagctgtt cgttggcatt
     4801 tttctgcaag ccaatcgcca gcgaaaccag gacgaccacc gcggcagtcc caatgatcac
     4861 gccaatggca gtcagggcga cgcgcccttt gcgccgcccc aggttgtcaa tgatcaatgt
     4921 cagtaaatca aagaaaccca tcgtgcttct ccagagagaa ttctctagcc cttgatcgga
     4981 ggtgcgactg gcactgcttc gctcgggcct tcctcgggga tcccttcaac cggcggcata
     5041 atctctccgc caccagaagg ctgggcactg tccagcccaa acaggccttt cagaaaccgc
     5101 accacttttt gaccaaaggt ttccgtctgt gctccgccat cggtgggaaa ttcacccccc
     5161 ggaatctcgc ccccaggaaa ttcgccgcca ttttgaggga agcctccatc cggcgggatc
     5221 cctccttcgg ggccgaccat catctcttcg accacgatcg agattgtgcg cgtgattacc
     5281 tgggcctggt tgaaatcatc ggtgtaatcc agcgtaatga gcaaatcgag tgggcccggg
     5341 gcttcgggga tcatggttgt atcgagggta tagtatccgc ccgggtcgag ggcacccacc
     5401 agggtgctgt tgttcatcat ctgcgcgccg ggcatgcctt cagccggctg ggctaccacc
     5461 cgcatactcc ccagaaccgc ctgtttccgt cccaggttaa ccacctgcag tggcagctgg
     5521 ttgggttgcc ctacgaaaaa ctgcccggca tcgcgataga agctgacctc gagctgcggc
     5581 accgaaaaaa ccagcagggt aatc
//
LOCUS       DATMXK010000076         2129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_156592, whole genome shotgun sequence.
ACCESSION   DATMXK010000076 DATMXK010000000
VERSION     DATMXK010000076.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2129
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_156592"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1683
                     /locus_tag="VN363_01450"
     CDS             <1..1683
                     /locus_tag="VN363_01450"
                     /EC_number="7.2.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015662 - P-type ion transporter activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0019829 - ATPase-coupled monoatomic cation
                     transmembrane transporter activity [Evidence IEA];
                     GO_function: GO:0043169 - cation binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWQ83198.1"
                     /translation="VLEPLSDGGPQAYRMIGDPTEGAMLVAAAKAWENIDELAVAFPR
                     RQEIPFDSSLKRMTTVHEITGVKDEGPSPFHDQTHVNKHAITIKGAPDLVLQLCTDYQ
                     TIDDQVKPLDDAMRASILAANDAMTNDALRVMGFAYRVEEDIPDELTREFAEQGLIFA
                     GLMGMIDPPRVEVKPALEKARGAGLRTIMITGDYPNTGRAVAESIGLLRKGDKVYTGT
                     DVNAMDDAALAEEVQRTAVFARVSPEHKMRIVDALRANDHVVAMTGDGVNDAPAIKRA
                     DIGIAMGITGTDVAKGAADMVLTDDNYVSIVAAVEQGRVIYSNIRKFVFFLLSSNIAE
                     VMIIFLATLFALPMPLTVIQLLWLNLLTDGAPALALAMEKGDPDIMEQPPRPKSESII
                     NSSMRLGMVIQTVAQTGVVLTAFILGLTWHLGDGVTMPEGTNLLSYVLQHDWSGVDVQ
                     TAETMAFVTLSLCELFRAYTVRSERASIFRLGVFSNKFMQYAVSLSIVLLLLVVNVPF
                     LQPIFNTHPLAVREWLIVLGLAVFPAIAEEFTKLYLRSRDAGEKKVREAQSA"
     gene            1777..>2129
                     /locus_tag="VN363_01455"
     CDS             1777..>2129
                     /locus_tag="VN363_01455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560212.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase"
                     /protein_id="HWQ83199.1"
                     /translation="MKETSQLQTQAINTIRFLAADGVQQAKSGHPGLPMGSAPMAYTI
                     WTRYLRHNPANPGWPDRDRFVLSGGHGSMLLYSLLHLTGYDLPLEELKRFRQWGSLTP
                     GHPEYGLTPGVESTTG"
BASE COUNT          437 a          635 c          566 g          491 t
ORIGIN      
        1 gtattagagc cgctctctga tggtggccct caagcttacc gcatgattgg ggatcccacc
       61 gaaggcgcca tgcttgtggc cgccgccaaa gcctgggaga atattgacga gttggcagta
      121 gctttccccc gccgccagga aatccccttt gattcctccc tgaagcgcat gaccaccgtc
      181 catgagatca ccggggttaa agatgaaggt ccttcaccct tccatgatca aacccatgta
      241 aacaaacatg ctatcaccat caaaggtgcg ccggacctgg tactgcagtt gtgcaccgat
      301 taccagacga ttgacgatca ggttaagccc ttagatgatg ctatgcgggc cagcatcctg
      361 gcagccaacg atgccatgac caatgacgct ctgcgggtga tgggttttgc ctatcgcgtc
      421 gaggaggata tccccgatga gcttacccgc gagtttgccg agcagggcct gattttcgca
      481 ggtctgatgg gcatgattga ccctccccgc gtcgaagtca agccggccct tgaaaaagct
      541 cgcggagcag ggctgcgcac cattatgatc accggggatt atcccaacac tggccgggca
      601 gtcgccgaaa gcatcggcct gctgcgtaaa ggcgataagg tttacactgg cacggatgtg
      661 aacgcaatgg atgatgccgc tctggccgaa gaggtgcagc ggaccgccgt cttcgcccgg
      721 gtctcccccg agcataagat gcgcattgtg gatgccttgc gcgccaatga tcatgtcgtt
      781 gcaatgaccg gcgatggagt caacgatgcc cccgccatca aacgcgccga tatcgggatt
      841 gccatgggca tcaccggtac ggatgtcgcc aagggcgctg ccgatatggt cctcacagat
      901 gataattatg tgagcattgt ggccgcggtc gaacagggcc gcgtcattta ttcgaacatc
      961 cgcaagttcg ttttctttct gctctcttcc aacatcgccg aggtcatgat catcttcctg
     1021 gcgacgctct tcgctctgcc aatgccgctg acggttatcc agctgctgtg gctgaatctg
     1081 ctcaccgatg gcgccccggc tctggctctg gcaatggaga agggcgaccc ggatatcatg
     1141 gaacagcccc cccgccccaa gagcgaatcg attatcaaca gctcgatgcg cctgggtatg
     1201 gttatccaga cggttgctca gacgggtgtg gtcctcactg ctttcatcct cggcctgacc
     1261 tggcatctgg gcgatggcgt caccatgccc gaaggtacaa atcttctatc ctacgtgctc
     1321 cagcacgact ggagtggggt ggacgtgcag accgccgaga cgatggcctt tgtcaccctc
     1381 tccctgtgcg aattgttccg ggcctacacc gtccgctctg agcgtgcttc aatcttccgc
     1441 ctgggtgtat tctcaaacaa atttatgcag tacgcagtca gtctttcgat tgttctgctg
     1501 ctgctggttg ttaatgtgcc tttcttacaa cccatattca acacccatcc gctggctgtg
     1561 agagaatggc tgattgtcct tggcctggca gttttcccgg cgatcgctga agagttcacc
     1621 aagctctatc tgcgcagcag agatgctggc gagaaaaagg ttcgcgaggc ccaatctgct
     1681 taatcaggag atccatccct atccgatgac ttgtgtgttc gagcagtcta cccgctcgaa
     1741 cacacagccg aaataaacgc tttttagagg tattctatga aagaaacttc ccagctccaa
     1801 actcaggcta ttaataccat tcgcttcctt gccgcggatg gggttcaaca ggctaaatct
     1861 ggtcacccag gcttgccgat gggcagcgcc cccatggcat atacgatctg gactcgctat
     1921 ctgcgccata atcccgccaa ccccggctgg ccggatcgcg accgcttcgt attgtccggc
     1981 gggcatggct ctatgctgct ctactccttg ctccatctca ctggctatga tcttccattg
     2041 gaggagctca aacgcttccg ccagtggggc agtctcaccc ccggacatcc tgaatatggc
     2101 ctgactcccg gcgtcgagtc cactaccgg
//
LOCUS       DATMXK010000077         2402 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_65106, whole genome shotgun sequence.
ACCESSION   DATMXK010000077 DATMXK010000000
VERSION     DATMXK010000077.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2402)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2402)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2402
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65106"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..840
                     /locus_tag="VN363_01460"
     CDS             <1..840
                     /locus_tag="VN363_01460"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ring-cleaving dioxygenase"
                     /protein_id="HWQ83200.1"
                     /translation="VNFDDPGTYHLYFGDETGTPGTVLTFFPFENARPGQVGAGQVGV
                     ISFAVPENSLPEWQQRLTGLNIPTSGIQERFGEQVLTLQDPDGLRLKLVGTPSAEQIP
                     GWQNGPVPAEHAIRGFHGATLLAGNLDSSARLLEEVFSFERLAQTSTYLRLKAPDSPA
                     GSIIDLLPVKERGVGGAGTNHHIAWRTPSDSDQLAWLDKLSGLGYRVTPIQDRQYFHS
                     IYFREDSGILFEIATDTPGFLVDETTENLGSKLRLPAWYEASREVIEANLPALNLPVP
                     VNS"
     gene            complement(939..1811)
                     /locus_tag="VN363_01465"
     CDS             complement(939..1811)
                     /locus_tag="VN363_01465"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class F420-dependent oxidoreductase"
                     /protein_id="HWQ83201.1"
                     /translation="MRIGIIYPQTEFGSDPIAIRDYAQTVEDSGFTHILAYDHVLGEN
                     AERPAQGYRPYTHFSAFQEPFVLFSYLAGFTRRIEFTTGILILPQRQTTLVAKQAATL
                     DVLSQGRLRLGVGLGWNAVEYQALNEDFHNRGKRSEEQVEVLRRLWTEPLVSFHGKYH
                     TIEDAGLNPQPVNGAIPIWFGGVADPVLKRAARLGDGWMPNYRTFEQAKPAIAAMQVY
                     LEEAGRSPDSFGIEGRVLYTPEQPKAYLEAAANWERVGASHLSINTMGLGLNTPGQHL
                     AAIQYIASELGLKS"
     gene            complement(1930..>2402)
                     /locus_tag="VN363_01470"
     CDS             complement(1930..>2402)
                     /locus_tag="VN363_01470"
                     /inference="COORDINATES: protein motif:HMM:NF021941.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="valine--tRNA ligase"
                     /protein_id="HWQ83202.1"
                     /translation="TDFNLVQEIVRSIRNLRAEKNVKPGRRIPAILVGGEQTKMLQTQ
                     SVAIASLAQLDGQSLQIVRQLSAKPEGHIGLVIGAVEIFLPLAGLVDVSEERTRLEKD
                     LASVNAQIQRLEGLLASSFAEKAPANVVQKEREKLEGYLETARRLKAQLAGLSE"
BASE COUNT          512 a          685 c          645 g          560 t
ORIGIN      
        1 gtcaatttcg acgatcctgg cacttatcac ttgtattttg gtgacgagac cgggaccccc
       61 gggacagtcc ttactttttt cccgttcgag aatgcccgcc ctgggcaggt tggtgcaggt
      121 caggtaggcg tgatttcgtt cgcagtaccc gagaattcat tgcctgaatg gcagcaacgg
      181 ttgactggtc tgaatattcc taccagtggt atccaggagc gcttcggtga gcaggttttg
      241 accttgcagg atccggatgg cttacgatta aagctggttg gaacaccatc agcagagcaa
      301 atccctgggt ggcagaatgg tccagtgccg gctgagcatg cgatccgtgg atttcatggc
      361 gcaaccctgc tggcgggaaa cctcgacagc tctgcccggc ttctcgaaga ggttttcagt
      421 tttgaacgcc tggctcagac gagcacttat ctgcggttga aagccccgga ttctccagca
      481 ggcagcataa tcgacctgtt gcctgtgaaa gagcgcggcg ttggtggggc aggtaccaac
      541 caccacattg cctggcgtac accctctgac tcagaccagc tggcctggct ggacaaactg
      601 tctggccttg gctatcgggt tacccctatc caggaccgcc agtatttcca ttcgatctat
      661 ttccgagagg attcgggaat cctattcgag atcgccaccg atactcctgg ctttctggtg
      721 gatgagacga ccgagaacct gggcagcaag ctgcgcttgc cagcctggta tgaggcgagc
      781 cgcgaggtga ttgaagccaa tttgccagcg ctgaatttac cggtccctgt gaatagctaa
      841 gcctgcttct gcagagcaaa tccagggtac tggctttgta aataaaaact gtccaggtcg
      901 agaaattgtg acctggacag tttattactt attctgaact agctcttcag accgagttca
      961 ctggcgatat actggatggc ggcaagatgc tgcccaggtg tattcaatcc caaacccatc
     1021 gtgttgatcg aaaggtgaga agccccgacc cgctcccaat ttgctgcagc ctccaggtat
     1081 gctttaggct gctcgggagt gtataacacc cgcccttcaa ttccgaaact atccggcgaa
     1141 cgaccggctt cttccaggta aacctgcatc gctgcgatcg caggctttgc ctgctcaaat
     1201 gtccgataat ttggcatcca tccatccccc aggcgcgccg ccctcttcaa cacgggatct
     1261 gcgaccccgc caaaccagat agggatcgct ccgttgactg gctgtggatt cagaccggca
     1321 tcctcgatcg tatggtattt cccatggaag gacaccagcg gttcggtcca caagcggcgc
     1381 aggacttcga cctgttcttc gctgcgcttg ccgcggttgt ggaaatcctc attcaaagcc
     1441 tgatattcga cagcattcca tcccagaccg actcccaggc gcaggcgtcc ctggctcagc
     1501 acatccaggg tcgcggcttg cttggcgacc agggtggtct gacgttgggg aaggatcagg
     1561 atcccggtgg taaattcgat gcgccgggta aaccctgcca ggtaactgaa cagcacaaac
     1621 ggttcctgga aagccgagaa gtgagtataa ggccggtaac cctgggcggg ccgctcagcg
     1681 ttttccccca gaacatgatc ataagccaga atatgcgtaa aaccagaatc ttcgacagtc
     1741 tgggcataat cccggatcgc aatcgggtcg ctgccgaact ctgtctgagg atagatgata
     1801 ccgatgcgca tagatgtgac tcaattcgtc tttgctgttg actcatgggg tagtgaagca
     1861 atcccacatg gatttcccat gccggagcat ggccttgaaa gatccaattc tcgcagttaa
     1921 tagccgtctc tattccgata accctgccag ctgcgccttc aaccggcggg cggtctccag
     1981 gtaaccttcc agtttttcgc gctccttctg gacaacattt gcgggagcct tttcggcgaa
     2041 ggatgaggcc agcagccctt ccaggcgttg aatctgggca ttgacactgg ccaggtcctt
     2101 ttccaaccgt gttctttctt cactcacgtc caccagcccg gccaggggta agaagatctc
     2161 cacagccccg ataacgagac caatgtgccc ctctggtttg gccgatagct gcctgacgat
     2221 ctgcaagctc tggccgtcca gctgagccag gctggcgatc gcaacgcttt gggtctggag
     2281 catcttcgtt tgctcaccac caaccaggat agccggtatc cgccgcccgg gcttgacatt
     2341 tttctctgcc cgcagattgc ggatcgaacg gacgatctcc tggaccaggt taaaatcagt
     2401 ag
//
LOCUS       DATMXK010000078         2151 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_65084, whole genome shotgun sequence.
ACCESSION   DATMXK010000078 DATMXK010000000
VERSION     DATMXK010000078.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2151)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2151)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2151
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65084"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..508)
                     /locus_tag="VN363_01475"
     CDS             complement(<1..508)
                     /locus_tag="VN363_01475"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83203.1"
                     /translation="MTNEPLRMQILNMIERGDISTGEGLRWLEALAKNQPTSASRGEL
                     DSDMPEADPDLPEPDFPDVETGAEGVAATILPPPSADHLPFDTARIRGWWVVPLWIGV
                     GVTVLAGLLMYWANLSQGIGFWFACASVPFILGLLLIVLAVQSRTARWLYLRVEQPGT
                     EWPRVIQFG"
     gene            complement(514..1782)
                     /locus_tag="VN363_01480"
     CDS             complement(514..1782)
                     /locus_tag="VN363_01480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83204.1"
                     /translation="MNQFTLPTSLAPEVIFDVTGSLQVQGWERPEIAIRAQSEALKVD
                     EKDDIVEIKCESNCAVRLPYGSTVHVTRVEGDARFKLLEDLLTIEKAMGHLSLRSVAE
                     TQIEIVQGDLDAKHITGNLSVDRAEGNTYIREVQGSCTLADVQGNLDVRNVESDIDIT
                     AQGNARLRLNTLDGSHYRVQAAGNLNCRIPDNASARFSLQAGAEEITLQLPNQPRSIK
                     SGRYTFTLAEGLAEIDLSAEGNLFLGSQTGLGGEENLDSLEAEIDSISESFSQQINQQ
                     IESQIESQMEALTRQLNEQMANISNVLGKSWLPAEESQRILERARQDSERATARAQER
                     MHRAQEKMEAKLEATRRRTELKAQAAQRRQQAANRRGRTFEWSPPSIPSAKESVSDEE
                     RLLILRMLEQKKITLAEAEELLAALEGKNG"
     gene            complement(1784..>2151)
                     /locus_tag="VN363_01485"
     CDS             complement(1784..>2151)
                     /locus_tag="VN363_01485"
                     /inference="COORDINATES: protein motif:HMM:NF021388.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF2089 domain-containing protein"
                     /protein_id="HWQ83205.1"
                     /translation="CPICGGELEVTRIHCSDCNTTIESRFTGGPFSQLSHEQLEFVET
                     FVRHEGKITRMETELGLSYPTIRNRLHEVIRALGYEPGGGEEAPGLSPEERQRILEDL
                     DKGKISAEKAMLLLQESGG"
BASE COUNT          407 a          621 c          627 g          496 t
ORIGIN      
        1 ggccaaactg gattactcgc ggccattcag ttccaggctg ctccacgcgc aggtaaagcc
       61 agcgggcggt ccggctttgt acagccagga cgatgagcag cagcccgagg ataaagggca
      121 cgctggcgca ggcaaaccag aagccgatcc cctgggacag gttggcccag tacatgagta
      181 atcctgccag cacagtgacg ccgaccccga tccagagcgg cacaacccac cagccacgga
      241 tccgggcagt gtcgaatggg agatgatctg cgctgggagg cggaagaatc gttgcagcga
      301 caccttcagc accggtttcc acatctggga aatctggttc cgggagatcg ggatccgcct
      361 caggcatatc cgaatcgagc tcgccccggg aggcagaggt gggctgattt ttcgcaaggg
      421 cttccaacca gcgcaaacct tctccggtgc tgatgtcacc tcgctcgatc atatttaaga
      481 tctgcatccg cagtggttca tttgtcatcg tctttatccg ttcttgcctt caagcgccgc
      541 cagcagctct tcggcttccg ccaatgtgat cttcttctgt tcgagcatgc gcagaatgag
      601 caggcgttct tcgtcgctga cggattcttt cgccgacggg atggatgggg gagaccactc
      661 aaatgtccgg ccgcgacgat tggcagcctg ctggcggcgc tgagcagcct gagctttgag
      721 ctcagtccgg cggcgagtgg cttccagctt ggcttccatc ttttcctggg cccggtgcat
      781 ccgttcctgg gcccgggctg tggcacgctc gctgtcctgg cgggcgcgct ccaggatacg
      841 ctggctttct tcggctggca gccaggattt ccccagcaca tttgagatat ttgccatttg
      901 ttcgttgagc tggcgggtca gggcttccat ctgcgattca atctggcttt cgatctgctg
      961 gtttatctgc tggctgaaac tttctgagat actatcgatc tcggcttcca ggctatccag
     1021 gttttcctcg ccgccaaggc cagtctggga gcccaggaag aggttaccct ccgcagacag
     1081 atcgatctcc gccagccctt cagccagggt aaatgtgtag cggccggatt tgatgctgcg
     1141 aggctggttg ggaagctgta aggtaatctc ttctgctccg gcttgcagcg agaagcgcgc
     1201 gctggcatta tctggaatcc ggcagttcag gttcccggct gcctggacgc gatagtggct
     1261 gccatccagt gtgttcaggc gcaggcgggc gttgccctga gcggtgatat caatatcgct
     1321 ttcgacgttc cggacatcca gattcccctg gacgtcggcg agggtgcagg aaccctggac
     1381 ctcgcgaatg taagtgttgc cctcagcgcg gtcgacgctc aaattgccag tgatatgttt
     1441 ggcgtccaaa tcgccctgaa caatctcaat ctgggtttcg gccacggagc gcaaggataa
     1501 atgccccatg gctttttcaa tcgtgagcag gtcttccagc agcttgaagc gggcatcgcc
     1561 ttcgacacgc gtgacgtgca cggttgagcc atagggcaaa cgcaccgcgc agttgctttc
     1621 acatttaatc tcgacgatat catctttttc gtctaccttg agagcctcgg attgggcgcg
     1681 tatggcgatc tcgggacgct cccagccctg tacctgcagg ctgccggtga catcaaagat
     1741 gacttctggg gccaggctgg tcggaagcgt gaactgattc atgctaacct ccactctctt
     1801 gcagcagcag cattgctttt tcggcgctga tcttgccctt atccagatct tccaggatgc
     1861 gctggcgttc ttccggagat aagccaggcg cttcttcccc gccgccgggt tcatacccga
     1921 gggcgcgaat gacttcatgc agccggttgc ggatagtggg ataagataaa cccagctcag
     1981 tttccatgcg ggtgatcttg ccctcatggc ggacaaaagt ctccacaaat tccagctgct
     2041 cgtgggagag ctggctgaat ggaccgccgg taaaacgact ttcaatggtc gtattgcaat
     2101 cgctgcagtg gatgcgggtg acttcgagct cacccccgca aattggacaa c
//
LOCUS       DATMXK010000079         5854 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_86924, whole genome shotgun sequence.
ACCESSION   DATMXK010000079 DATMXK010000000
VERSION     DATMXK010000079.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5854)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5854)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5854
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86924"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..487
                     /locus_tag="VN363_01490"
     CDS             <1..487
                     /locus_tag="VN363_01490"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWQ83206.1"
                     /translation="PVIFVCENNLYATEVAFSYASKSANVAGRAAGYGLPGVVVDGND
                     VLAVYQAAKEAVQRARAGEGPTLLECQTYRTRAHSEGMRDAGYRTVEEVERWKARDPI
                     QLWKSHMLEQGLVREVEISALEAEVLKRAEDAAQFARQSPLPDPSTVYDHLYSQEVQH
                     G"
     gene            480..1454
                     /locus_tag="VN363_01495"
     CDS             480..1454
                     /locus_tag="VN363_01495"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWQ83207.1"
                     /translation="MAEMTFTDAARAALAEEMERDPMIFVVGEGIGPRGGNFNTTVGL
                     HELYGDMRLRDTPISERGFTGLCTGAAVLGARPVVDFMFLDFELDALGEIINQTSRLR
                     WMSNGQVKVPIVIRGGVGVASTGPHHSSNLFSVFMHIPGLHVVTPVFPADAKGLLKTA
                     IRSDDPVIFLEHKKLMKLKGPVPDGEHLVPFGKAAIVRPGTDVTIVAISYPVVEALSA
                     SRLLEADGISAEVIDPRTLAPLDIDTILKSVAKTHRLLVVDEDFAPCGAAAEIATQVM
                     ERAFYELDAPVRRLNGAFIPAPYSPPLEAAVVPNAASIAQAVRALIQE"
     gene            1470..2750
                     /locus_tag="VN363_01500"
     CDS             1470..2750
                     /locus_tag="VN363_01500"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWQ83208.1"
                     /translation="MAYDVTLPRLGWDMEEGSLASWLKQEGEFVKRGELLFTVEGDKA
                     IQEIEALDTGYLRILPDGPQPGVKVPVGTLLGYLVPESERDSFKFPGSDSKQHSTTAA
                     WQDPPTMAVPEAAVPPDVLDAKARRIYISPYAKRLAEGLSVDWERLQGSGRAGRIMAA
                     DVEKAAAQPVTQKPDRVAIPKPPPAPLPAIPGMVTRQPMSQTRKKIAEHMAASAHTVA
                     PVTLTAEVDATEMVTLRKQLKADAALDGLPVPSYNDMLAKIAAQALLEHPHMNAQIVG
                     DEILFSNSAHIAVAVDTERGLLVPVLRDVQAKSLRQVAREAAALIEKTRQGTVAYADL
                     QGATFTITNLGMFEIEAFTPIIDLPQCAILGVGQIIAKQVVTDAELELVEIRQRMVLS
                     LTFDHRLVDGAQAARFLQRIKRLVERPYVWLVGS"
     gene            complement(2816..4171)
                     /locus_tag="VN363_01505"
     CDS             complement(2816..4171)
                     /locus_tag="VN363_01505"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrolipoamide acetyltransferase family
                     protein"
                     /protein_id="HWQ83209.1"
                     /translation="MSKYIDVIMPVLGMSQDSGKLVRWIKKSGERVEKGEPLMEVETD
                     KATVEIEAPASGWLTQISAQEGDDIPVTRVIAVIQDESGEGAPAPQVTKVEPAPALKP
                     GPLTGMAPLSISPLAARIAAENHLDPSQIKPNGGRIEKADVLAYLGNVSHPESVIRPR
                     RILASPKARRLAKESGLVLADLHGSGPEGAILAVDVLSIHTVSPDAGPALGTPVPGEA
                     GSPLPTSAIPTELPMSTTWARMIERISASWPATPHFYLVREVNASRMVAWGAQAQAQN
                     ENKITVTDLLVRLCAAALAKHPRINAQYHNGKLLQMPEVNIGLAVAVEEGLVVPVIKQ
                     ADRLSLSEVAAARKALVERARAGKLRLEDMQGGTFTISNLGMYDIDAFNAMINPPQAA
                     ILAVGRIADRVVAVNGQPAVQPMLTLSVSFDHRVVDGARGAEFLKTLAEWIEEPLALL
                     H"
     gene            complement(4173..5213)
                     /locus_tag="VN363_01510"
     CDS             complement(4173..5213)
                     /locus_tag="VN363_01510"
                     /EC_number="1.2.4.-"
                     /inference="COORDINATES: protein motif:HMM:NF006667.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-ketoacid dehydrogenase subunit beta"
                     /protein_id="HWQ83210.1"
                     /translation="MSTLEISRPDQRSSDSLEPEMKEITLSQAVHDALVEEMRRDPNV
                     FIIGEDVAEAGTPFKVMQGLVDEFSTQRVIDAPISEPGFCSLGVGAAMTGMRPIVDIM
                     FGDFLGLVMDAIANQAAKVHYMSGGKLKVPIVFRTTLGATRRAAAQHSQSLQAWPSHI
                     PGLKVVLPSTPYDAKGLLKTAIRDDNPVVFIEDKLSWQMKGLVPVEEYTLPFGVADIK
                     RAGTDITLVATSSMVQVALGAAEKLATIGISAEVIDPRTTSPLDVNTLVESAKKTGRA
                     IVLDEGYERYGVTAELASVIADGAFYYLDAPVKRMGAMNVPVPFSPALEDQTVPTPDT
                     VLAMAKLLCERL"
     gene            complement(5200..>5854)
                     /locus_tag="VN363_01515"
     CDS             complement(5200..>5854)
                     /locus_tag="VN363_01515"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016624 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, disulfide as
                     acceptor [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWQ83211.1"
                     /translation="PETGNLGANAIVGAGSGLATGAALSMKLRGTHQVSVAFFGEGAM
                     GQGLVYEVMNMAALWKLPVVYVCENNHYSEYTYFKETLAGELRNRPVAFGIPVVEVDG
                     QDVRSVYLAAQGLIEQARSGDGPGFLICDTYRYYGHHVGDINRGYYRSKEEEEFWHMN
                     RDPLTLMSTWLSEQKAVPASVFAEIERKVGDEIAHGVEFALQTPYPDASEVYDNVYA"
BASE COUNT         1218 a         1675 c         1689 g         1272 t
ORIGIN      
        1 gccggtgatc ttcgtgtgtg aaaataattt atatgctacc gaagtggcct tcagctatgc
       61 cagcaagagt gctaatgtgg ccgggcgggc agcaggttac ggactgcccg gcgtggtggt
      121 ggatggcaac gatgtgctgg cggtttacca ggcggctaag gaagctgtcc aacgcgcccg
      181 ggccggggaa ggcccgacct tattggaatg ccagacttac cgcacgcgag ctcattctga
      241 gggcatgcgc gatgccgggt atcgcaccgt ggaggaagtg gagcgctgga aagcccgcga
      301 tccgatccaa ctgtggaaga gccacatgct ggagcaaggt ctggtgcgcg aggtggaaat
      361 tagcgccctt gaagcagaag tgttgaaaag ggctgaagac gctgcccagt ttgctcgcca
      421 aagcccactg cccgacccga gtactgttta cgatcatctt tattcgcaag aggtgcagca
      481 tggctgagat gacctttacc gatgcagccc gagccgccct ggcagaagaa atggaacgtg
      541 atccgatgat ctttgtggtt ggggaaggga ttggcccgcg cggcggcaat ttcaacacca
      601 ctgtaggcct gcatgagctg tatggggata tgcgtttgcg cgatacaccg atcagcgaac
      661 gtggcttcac cggcctttgc accggcgctg ccgtactggg cgctcgcccg gtggtggatt
      721 tcatgttcct ggattttgag ctggatgctc tgggcgagat catcaaccag accagccgtc
      781 tgcgctggat gagcaacggc caggtcaaag tgccaatcgt aatccgtggc ggggtaggag
      841 tggcttccac cggcccgcat cattcatcca atttgttctc agtcttcatg cacatccccg
      901 gcctgcatgt ggtcacgcct gtctttccag ccgatgccaa aggcttgttg aaaaccgcta
      961 ttcgcagcga tgatccggtt atcttcctgg agcataaaaa gctgatgaag ctcaaaggcc
     1021 cagtcccaga cggtgagcat ctggtgcctt tcggcaaggc ggcgatcgtc cgccccggta
     1081 ccgatgtgac cattgtcgct atctcttatc cggtcgtcga agccttgagc gcttccaggc
     1141 tgctcgaagc agacggcatt tcggccgagg tgattgaccc gcgcaccctg gcgcccctgg
     1201 atatcgacac gatcctgaaa tcggttgcca aaacccaccg cctgctagtg gtggatgagg
     1261 actttgcccc atgtggggct gcggctgaga ttgctaccca ggtgatggaa cgtgcctttt
     1321 acgaactgga tgccccggtg cgtcgtttga atggcgcttt tatccccgcc ccgtacagcc
     1381 ctccgctcga agcggcggtg gtgcccaacg cggccagcat cgcccaggct gttcgggcgc
     1441 tcatccagga atgaggtcag gagacgatca tggcttatga tgtcacacta ccccgcctcg
     1501 gttgggacat ggaagaagga agtctggcct cctggctcaa acaggaggga gagttcgtaa
     1561 agagaggtga gctgctcttt acggtggaag gcgataaagc tatccaggag atcgaagcac
     1621 tggatactgg gtatttacga atcctgccag acggccctca gccaggggtc aaggtcccgg
     1681 tgggcacatt gttggggtat ctggttccgg agagtgagcg cgattccttt aaattccccg
     1741 gaagtgactc caaacaacat tctacaacag ccgcatggca agacccgccg acgatggcgg
     1801 tgcctgaagc tgccgtcccg ccagatgttt tggatgctaa agcgcgccgg atttatatca
     1861 gcccgtatgc caaacgcctg gcggagggcc tgagtgtgga ttgggaacgt ctgcagggta
     1921 gcgggcgcgc aggacggatc atggccgcag atgtcgaaaa ggctgctgcc caaccggtca
     1981 cccagaaacc tgaccgggtt gctatcccaa agcctcctcc tgctccgcta ccagcaatcc
     2041 ctggtatggt tactcgtcag ccgatgagcc agacgcgcaa gaagattgct gagcacatgg
     2101 cagccagcgc ccataccgta gctccggtga cgctgacggc agaggtggat gccaccgaga
     2161 tggtgacatt acgcaagcag ctcaaagccg atgcagcgct ggatggcctg ccggtgccct
     2221 cgtataacga tatgctggcc aagatcgccg ctcaggcgct gctggaacac ccgcacatga
     2281 atgcacagat tgtgggggat gaaatcctgt tttccaattc cgcacatatc gccgtagcag
     2341 tcgataccga gcgcggttta ctggtgccag tcctgcgtga tgtgcaagcc aaaagcctgc
     2401 gccaggtagc gcgcgaagcg gctgcgctga tcgaaaaaac ccgccagggg acggtcgcct
     2461 atgccgatct gcagggggcg accttcacaa tcaccaatct ggggatgttt gagatcgaag
     2521 cttttacccc gatcatcgac ctgccgcaat gcgcgattct gggcgttggg cagatcatcg
     2581 caaaacaagt agtcaccgac gccgagctgg agctggttga aattcgccag cgcatggtgc
     2641 tcagcctgac ttttgaccat cgactggtag atggagctca ggcggcgcgc ttcctgcagc
     2701 gcatcaagcg cctggtggaa agaccatatg tgtggttagt ggggtcttaa aaagtgtgag
     2761 gcaaagcggt aaaatagtcg gtcgatgctg tatgttgctc cgaccggcta ttcgcttaat
     2821 ggagcaaagc cagcggttct tcgatccact ctgccagggt cttcagaaat tcagcaccgc
     2881 gggcaccatc taccacacgg tgatcgaagg agacacttag agtcagcata ggctgcacgg
     2941 caggttgccc gttcacggct accacgcggt ctgcaatgcg gcctacagcc aaaatggctg
     3001 cctggggcgg gttgatcatg gcgttgaacg catcaatatc atacatgcca aggttactga
     3061 tcgtaaacgt gccgccttgc atgtcttcca ggcgcaactt gccagcccgg gcgcgttcca
     3121 ccagggcctt gcgcgccgcg gcgacctcac tcaggctcag gcgatcggct tgtttaatca
     3181 ccggtacgac cagtccttct tctaccgcaa ccgccaggcc aatattgacc tccggcattt
     3241 gcaagagctt gccattgtgg tattgagcat tgatgcgcgg atgtttcgcc agggctgccg
     3301 cgcataagcg taccagcaga tcggtcactg tgatcttatt ttcgttctgt gcctgggctt
     3361 gcgctcccca ggcgaccatg cggctggcat tgacttcgcg taccaggtaa aaatggggcg
     3421 tagccggcca gctggctgaa attcgctcga tcatgcgcgc ccaggtcgta ctcatgggga
     3481 gctctgtggg gattgcgctg gtgggcaaag gtgaacctgc ttctccaggt acaggtgtac
     3541 ctaaagcagg gcctgcgtct ggggacactg tgtggataga caggacatcc acagccaaga
     3601 tcgctccttc cggcccactg ccatgcaggt ctgccaaaac cagaccgctt tctttagcca
     3661 gacggcgagc ctttggagaa gccagtatcc gtctcggccg aataaccgac tcagggtgac
     3721 tgacattgcc taaataggct aatacatccg ctttctcaat ccgccctccg tttggtttga
     3781 tctggctggg gtccaggtgg ttttcggcag ctatccgggc tgccagcggg ctgatggaga
     3841 gcggcgccat gccagtaagg ggtcctggtt tgagcgcagg tgcgggctca accttagtca
     3901 cctgaggagc aggagcgcct tcaccagatt cgtcctggat tacggcaatg acccgggtga
     3961 ccggaatatc gtcaccttcc tgggccgaga tctgagtcag ccagccggag gcgggtgctt
     4021 caatctctac agtagccttg tcagtctcca cttccatcag aggctcgcct ttttctaccc
     4081 gctcgccaga cttctttatc caacgtacca gcttgcccga atcttgcgac attcccaaaa
     4141 ctggcatgat cacatcgatg tacttgctca tgttacagcc tctcgcagag cagtttggcc
     4201 attgcaagta ccgtgtctgg cgttggcaca gtctggtctt ccagggcggg ggagaacggt
     4261 accggcacgt tcatggcgcc catacgtttt accggcgcat ccaggtaata gaaggcccca
     4321 tcagcgatga ctgaagccag ctcggcagtc acaccgtagc gctcatagcc ttcgtcgagt
     4381 acgatcgccc gcccagtctt tttagccgat tcaaccagag tgttgacatc cagtggggat
     4441 gtcgtccgcg ggtcaatcac ttcggcgctg atcccgatgg tggccagttt ttcggcggca
     4501 cctaaggcta cttgcaccat actgctggtc gccaccagag tgatatctgt gccagcacgc
     4561 ttgatatctg ccacaccgaa gggaagagtg tactcctcca ctggcaccag acctttcatc
     4621 tgccagctca gtttatcttc aatgaacact accgggttat cgtctcggat ggcggtcttg
     4681 agcaagcctt tggcgtcgta gggtgtggat ggtaagacta ctttcaagcc ggggatatgg
     4741 ctgggccagg cttgcaggct ctggctgtgt tgagctgcag cccgtcgggt agcgcccagg
     4801 gtggtacgga agacgatcgg caccttgagc ttgccgccgg acatgtaatg tactttagca
     4861 gcctggttgg cgatggcgtc catcaccagc cccaggaaat caccaaacat gatatccact
     4921 atcgggcgca taccggtcat cgcggcaccc acacccaggc tgcaaaaacc aggctccgag
     4981 atgggtgcat cgatcacccg ctgggtgcta aattcatcca ccaggccctg catcaccttg
     5041 aacggtgtac cggcttcggc tacatcttcg ccaataatga atacatttgg atcgcggcgc
     5101 atctcctcca ccagggcgtc gtgcacagcc tggctgaggg taatttcttt catttcgggt
     5161 tcgagggaat ccgagctcct ttgatcgggg cggctgatct caagcgtaga cattgtcgta
     5221 tacctcactg gcatccgggt agggggtctg cagggcaaat tccaccccgt gtgcgatctc
     5281 atccccaact ttacgttcaa tctctgcgaa aacagaagct ggaactgcct tctgctcgct
     5341 caaccaggtg ctcatcaatg ttagtggatc gcgattcatg tgccagaatt cttcttcttc
     5401 cttggagcgg taatagccgc ggttaatatc ccccacatga tgtccataat aacggtatgt
     5461 atcgcagatc aggaaacccg ggccgtctcc agaacgcgcc tgttcaatca gcccctgagc
     5521 agccaggtac acactgcgaa catcttgccc gtccacctcc accactggga taccgaaggc
     5581 taccggacgg ttgcgcaatt cgccggccaa cgtttccttg aaataggtat attcagagta
     5641 gtggttattt tcgcacacgt agaccactgg cagcttccac agggcagcca tgttcatcac
     5701 ttcgtatacc aggccctgtc ccatggcgcc ctcaccaaag aacgccacac tcacctggtg
     5761 agtaccgcgc agtttcattg aaagcgctgc cccggtggcg aggccgctgc ctgccccaac
     5821 gatggcattg gcgcccagat ttccggtctc gggg
//
LOCUS       DATMXK010000080         3191 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_8093, whole genome shotgun sequence.
ACCESSION   DATMXK010000080 DATMXK010000000
VERSION     DATMXK010000080.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3191)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3191)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3191
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_8093"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VN363_01520"
     CDS             complement(<1..425)
                     /locus_tag="VN363_01520"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWQ83212.1"
                     /translation="MSNLYRSQKDSMVGGVCGGLGQYLGIDPTFVRLFFVLMALAGNG
                     IGVFIYLLLWIILPLDQPRRKQNDHLSENVRAGSEEILDQARAVGEDIKNIVVRPHPN
                     ASLIIGGALIILGIFYLLDHLQVEWLRWLNFDLVWPLLL"
     gene            complement(433..1074)
                     /locus_tag="VN363_01525"
     CDS             complement(433..1074)
                     /locus_tag="VN363_01525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83213.1"
                     /translation="MLKWFESRVLWGSLLILGGILFLLQNLGVFEIGSLFWGLLFGLA
                     GVFFLSIYFQNRASWWSIIPGFTMFGIALAIVLDQLMPEVGNLIGGSLFLGTLGLGFL
                     FVYQADRQNWWALIPMGVLLTLAVVSGLDEVSGLDTGGIFFLGLGLTFALVATLPKGI
                     DTRWAWIPAAILIGLGLFIFAAAQAVLNYVWPLALILVGGYMIYRTMRFNRTG"
     gene            complement(1106..2698)
                     /locus_tag="VN363_01530"
     CDS             complement(1106..2698)
                     /locus_tag="VN363_01530"
                     /EC_number="6.3.4.21"
                     /inference="COORDINATES: protein motif:HMM:NF006695.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004516 - nicotinate
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate phosphoribosyltransferase"
                     /protein_id="HWQ83214.1"
                     /translation="MNQQKRKTAEGILFTDMYQFTMAQLYYRMGLQEKLVQFDHYFRS
                     YPDYGTHKAGYCIAAGLEWLIDWMQETHFEPEEIEYLRSQKTRTGSPMFADDFLDWLS
                     KQGDFSALNLFAIPEGRVVHPNVPLTVVRGPLAMAQILETALLNQINFQTLIATKAAR
                     MREIGHGQLMMEFGLRRAQERGGNAGARAALIGGADYTSNVGVSALLGIQPKGTHAHS
                     MVQLFIAMGQSELDAFRAYADVYPDDCLLLVDTIDTLESGVPNAIQVFQELRSKGHQP
                     VGIRLDSGDLAYLSIQASRLLDEAGFPDVSITLSNNLDELIIWQIITQIQEEAPRYGV
                     DADHLVKRLGYGIGTRLITSWGEPALGGVYKLVAVHHENSWQPAIKISESPEKTPNPG
                     YKNVWRLYDQRGKATADLLSLQEEDPRKMERIVLRHASNQTKFRVMEKITISEIEPLL
                     VQYLAEGQLVADLPDLETLRARRVADVARLDAGVRRLMYPHIYHVSLTQKLWDLKQQL
                     ITSALNVEDLLHIPIDPEKDLT"
     gene            complement(2716..>3191)
                     /locus_tag="VN363_01535"
     CDS             complement(2716..>3191)
                     /locus_tag="VN363_01535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83215.1"
                     /translation="REQTRGETKEVELKPKAEVFVVPFGPDQALQWDFSLAPSIPLEL
                     ILETGANEASADLTGLLVERLKLSSGASSTHIILPAAAGYTEVKVDSGVTSVRLTVPA
                     EVAGRIRYAGGLSNLQIDKQRFIQNGNNYESADYSTAANKVDISIDTGMASIVID"
BASE COUNT          786 a          897 c          790 g          718 t
ORIGIN      
        1 attagcagca ggggccatac caggtcgaaa ttcaaccagc gcagccattc cacctgcaga
       61 tggtcaagca gataaaatat tcccagaata attaacgctc cgccgatgat caggctggca
      121 ttggggtgtg gacggacaac aatatttttg atatcctcgc ccacagcccg cgcctgatcc
      181 aaaatttctt cactgccagc ccgtacattt tcactcaaat gatcgttttg ctttcgtctg
      241 ggttggtcca gtggcaaaat gatccacagc agcaggtaga taaatacgcc aatcccattg
      301 ccggccagcg ccatcaacac gaaaaacaac cgcacgaagg tgggatcgat gccgaggtac
      361 tggccaagcc cgccgcatac cccaccaacc attgagtcct tttggctgcg atacaggttg
      421 ctcatctgtg gattatcctg tccggttaaa gcgcatcgtc cgatagatca tataaccgcc
      481 caccagaatc agtgccaacg gccagacgta attcaacaca gcctgcgctg cggcaaatat
      541 gaataaaccc agcccgatca ggatcgcagc cggaatccag gcccagcgtg tgtctatccc
      601 tttgggtaaa gtggctacca gtgcgaaggt caagcccagt cccaggaaga agatcccacc
      661 cgtatccagc ccggagactt cgtcgagccc acttacgacc gccagggtca gtaagactcc
      721 cattgggatc agagcccacc aattctggcg gtcagcctga taaacaaaca ggaagcccag
      781 gccgagcgtg cccaaaaaca gtgaaccacc gatgagattt ccaacctcag gcattaactg
      841 atcaaggaca atcgcgagag caattccaaa catggtaaaa cccggaatga tactccacca
      901 ggatgccctg ttttggaaat agatagacag gaagaaaacc cctgccaggc caaacagcag
      961 accccagaac agactcccga tctcaaatac acccaggttt tggagcagga aaagaatgcc
     1021 tcccaggatg agcagtgaac cccacagcac tcgtgattca aaccatttta acattgtgaa
     1081 ctccttatct tcgctccagg gatatttagg tgaggtcctt ctcaggatcg attggtatat
     1141 gcagcaaatc ttctacattc aacgccgagg taatcagctg ttgtttcaag tcccataatt
     1201 tctgggtgag ggaaacatgg taaatatgag ggtacattaa ccgccggacg ccggcatcca
     1261 gacgggctac atctgcaacc cgccgggcgc gtaatgtttc cagatcgggc aagtccgcca
     1321 ccagctggcc ttcagccaga tattgcacga gcagcggctc gatttccgag atggtgattt
     1381 tctccatgac gcggaacttg gtctggttgc tggcatggcg taagacaatc cgctccattt
     1441 tccgcgggtc ctcttcctgc aaactgagca aatccgctgt tgccttgccg cgctggtcgt
     1501 acaatctcca gacattttta taacctggat taggggtctt ttcaggagat tcggatattt
     1561 tgatcgctgg ctgccagctg ttttcatgat ggacggctac cagtttgtag acccctccca
     1621 gcgcaggttc accccaggag gtgatcaatc gggtgccaat gccatacccc aggcgtttca
     1681 ccagatggtc ggcatctaca ccataacggg gggcttcctc ctggatctgg gtgatgatct
     1741 gccagataat cagctcgtcc aggttgttgg acagggtgat cgaaacatcc ggaaagccgg
     1801 cttcatccaa caatcgcgag gcctggatgc tcaaatatgc caggtcgccc gagtccaggc
     1861 ggataccaac cggctggtgg ccttttgaac gcagctcctg gaaaacctgg atggcatttg
     1921 gaaccccgct ctcaagcgta tcaatcgtat cgaccaaaag caaacaatca tccggataga
     1981 catccgcata agccctgaaa gcatccagct ctgattggcc catggcgata aatagctgga
     2041 ccatgctgtg agcgtgggtg cctttcggtt ggatccctaa taaagctgag acgcctacat
     2101 tcgatgtgta gtctgcccca ccgatcaacg cggcccgggc tccggcgttg ccgccgcgct
     2161 cctgggcccg ccgcagacca aattccatca tcagctgccc gtgaccaatc tcgcgcatgc
     2221 gcgcggcttt ggtggctatc agagtttgga aattgatctg gttcaacagg gctgtctcga
     2281 gaatttgggc catcgccagc ggaccgcgga ctacagtcaa ggggacattc gggtgcacga
     2341 cccttccctc gggtatcgcg aacaggttga gtgccgaaaa atcaccctgc ttgctcagcc
     2401 aatccagaaa atcatccgca aacataggcg atccagtccg ggttttctgg gatcgcagat
     2461 attcgatctc ctccggctcg aaatgcgttt cctgcatcca atcgattaac cactccaggc
     2521 cggccgcgat acaatatccg gctttgtgag tgccataatc ggggtagctg cggaagtaat
     2581 gatcgaactg gaccagtttt tcctgtaagc ccattcgata atagagctga gccatcgtaa
     2641 actggtacat atcggtaaac aagataccct cagctgtctt gcgtttctgc tggttcaaag
     2701 atatctcctt gtagatcagt cgatgacaat tgaagccatg ccggtatcga tgctgatgtc
     2761 aactttgttg gcagcagtac tgtaatccgc tgattcataa ttattcccat tttggataaa
     2821 acgttgctta tcgatctgga ggttggacag accaccggca tagcggatcc gtccggctac
     2881 ctcggctgga accgtcagcc gcacggaagt aaccccagaa tccaccttta cctcggtata
     2941 acctgccgca gcaggtaaga taatatgagt ggagctggct cctgagctta gctttaacct
     3001 ttctaccagc aagccagtca aatctgcgct ggcttcgttg gctccggttt ccagaatgag
     3061 ctcgaggggt atactgggcg ccagactgaa atcccattgc agtgcctggt ctggtccaaa
     3121 ggggacaacg aagacctccg cttttggctt caactcgact tccttcgttt ccccacgggt
     3181 ttgctcgcgg g
//
LOCUS       DATMXK010000081         7711 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_122106, whole genome shotgun sequence.
ACCESSION   DATMXK010000081 DATMXK010000000
VERSION     DATMXK010000081.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7711)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7711)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7711
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_122106"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VN363_01540"
     CDS             <1..292
                     /locus_tag="VN363_01540"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83216.1"
                     /translation="PYSYPVPGAVEQAKERIQETIYQYLTIAQAERYIQSNIGKPAPY
                     FGEIYLRLGELYEQEGDLLAARELYESIPELFPNRADLLELAETHLKRLGDS"
     gene            350..1927
                     /locus_tag="VN363_01545"
     CDS             350..1927
                     /locus_tag="VN363_01545"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWQ83217.1"
                     /translation="MNLRRKVIQVTGILMLISAVVMPFSSTGLADTSTLTGHQRETLQ
                     MYAQDTWQSFLAMLDPDTGLPADNVSVDGERSGFTSPTNIGMYVWSTLAARDLQIIQP
                     LEARQRIGTVLNRLEMMERHTDSGMYYNWYDPATGAKLTIWPPSGDPVYPFLSSVDNG
                     WLASALIMITNAVPQLRDQAQVILNSMDFSCYYDPNARGSDYGAGLLRGGFWTVEDAP
                     PWATAPLDNYCDKGPDVIYTGHHYGTFNTEPRIASYIGIALGQIPPEHYFAGWRTFPP
                     TCDWSWPEMAPQGETHTYLGIEVYEGSYQYRGMNIVPSWGGSMFEALMVPLVVPEAEW
                     GQKSWGINHPLYVQAQIEHGLEEAQYGYWGFSPSNNPAGGYREFGVDALGMDTGGYTS
                     DLERTSVDYGFADPSGIGYCPGREPQPLPETYGQGVVTPHASFLALEFAPEATLENLD
                     NLRRDFEIYSWGGFFDAVNVSNGDLSTYYLALDQGMIMASISNALRNDRLQHYFTHGA
                     VEEFMRPLMEMEEFSAH"
     gene            2087..4453
                     /locus_tag="VN363_01550"
     CDS             2087..4453
                     /locus_tag="VN363_01550"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl transferase family 36"
                     /protein_id="HWQ83218.1"
                     /translation="MPQFQTEYGYFCDDGQEYIITRPDTPVPWINVISNQNYGLVLSQ
                     SGGGYSWLSHASLNRITRWEQDLIRDEWGKFIYLRDMDSGEFWSPAWQPAGRKLDEYC
                     VRHGRGYSIIESRCAEIETRLTYFVPRFDPCEIWQLKINNRSSVSRRFQVFTYFEWLL
                     GAAPDWHREFHRLFVRTWYNQQKGALLASKVLWDLPGETGPHWNRDWAYVAFHSSSIH
                     PSGYDAYKDAFLGRNQSLAAPDALNEGRSLNTTGSWGDPIGSLQVEIRLEADQELELI
                     FTLGATETESQALDLAEQYKNPHAVQVAFDEVKSFWQGIGKGLIVETPDHALNLMANT
                     WLPYQVISGRLWGRTAYFQTGGAFGFRDQLQDSLLWLLLGQPEQTLAQIRLHASHQYT
                     DGIVLHWWHPLAEVGLRSEYSDDLLWLPFVVLHYIYETGDLTCLEEMIPFFDGGSATL
                     HEHCKRAFTVALERRSPHGLPLILKGDWNDGLNAVGAGGKGESIWMALFLYYLLTRWS
                     ELPDLDEEMCLRYKAEVKALRDSTEIYGWDGAWYRRATTDDGQHIGSAQNNEGQIFLN
                     TQTWAVLSGLATPDRAGQAILSARQHLYTAYGPLLLAPAYSQPDPEIGYLTRYAPGVR
                     ENGGVYVHAACWAVMAERKAHGVQAAYDLWRSFCPPVRGMEPEAYTAEPYVMPGNVDG
                     PDSQFPGRGGWTWYTGSAAWYYRALIEGVLGVEATKEGLRVQADLPDGWDRYHVKRLF
                     RGATYDIHIRRANSGEQTGCTVDDRPWQGETLPILEPGTVHLIKFTCR"
     gene            4467..6602
                     /locus_tag="VN363_01555"
     CDS             4467..6602
                     /locus_tag="VN363_01555"
                     /inference="COORDINATES: protein motif:HMM:NF021596.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWQ83219.1"
                     /translation="MIQSMPLQPYTPGTYYNHLIFDNSLSDGGYFFSHAVAIAPSQLE
                     AIDCKIPVAADRFLSPPNSLRLSWLSQTGGDWLAELYSETWLNKTRLLSGDTLSFWGY
                     SEEEIPVRLLPMIRLKIKDGPLTSPLQMTGIVPDLPARHWQQINIPLEAFPNETFNVD
                     FSRIEKIIFTQSLDDNFPHTLYIDEVKISDSAMVGMGRPPSGLSAVAYDCHVDLSWRA
                     VNDPAVMYYQIYRSNNGQDFLPVGIQNPTFNRYVDYVGQSQRKLFYRITSVSSNYSES
                     PASQIVSGTTHPMTDDELLSMVQEACFRYYWENAHPNAGLALENVPGDENLIALGASG
                     FGIMALIVAVWRGFVARETAIERLLVILSFLEIADRFHGVWPHFLDGRTGKVIPVFGK
                     YDNGGDLVETAFMMQGLMAARQFFANEEEGEKQIRSRITSLWETVEWDWYRQSADSEF
                     LYWHWSPDYGWHIGHPLTGWNETLIAYLLAIASPTHPVPAAMYYSGWASSSETAQRYR
                     QAWGQTTQGDLYRNRNVYYGIQLPVGVGSGGPLFFTHYSFLGFDPRNKRDRFGDYFES
                     NRAIVLINRAYCIENPGKYQGYGENFWGLTASHDHTGYLAHEPSPRADNGTITPTAAL
                     SSFPYTPEGSMAALKHFYYDLGDKMWGIYGFRDAYNPTVNYVSNIFMGLNQAPITVMI
                     ENYRSGLLWNLFMSNPEITSMVKRIGLVK"
     gene            complement(6844..6915)
                     /locus_tag="VN363_01560"
     tRNA            complement(6844..6915)
                     /locus_tag="VN363_01560"
                     /product="tRNA-Lys"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(6881..6883),aa:Lys,seq:ctt)
     gene            7066..>7711
                     /locus_tag="VN363_01565"
     CDS             7066..>7711
                     /locus_tag="VN363_01565"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ83220.1"
                     /translation="MSPGLIDTVTEKDTLLVVEDNDALREGLREMLSLEGFAVLAAAN
                     GRAALEQMSTLTPDLIVSDISMPEMDGIAFFKAVRERPDWLTIPFLFLSARGEKQDIL
                     RGKNLGAEDYLVKPLTRDELVTAVRSRLERFEQLRLAQLQRAYEASLTALANAIDLRD
                     RYTRGHVERVTAYCLAMASKLRWPEMNLDHLRFGAILHDIGKIHIPENILFKKTA"
BASE COUNT         1789 a         2037 c         2077 g         1808 t
ORIGIN      
        1 tccttactct tatcccgtgc ccggcgctgt ggaacaagcc aaagagcgca ttcaggaaac
       61 catctatcaa tacctgacca ttgcccaggc ggagcggtat atccaatcca atatcggcaa
      121 acctgcaccc tacttcggag aaatctactt gcgattgggc gaattatacg agcaggaggg
      181 agatctgctc gcagcgaggg aactctatga atctataccc gaactttttc ccaaccgtgc
      241 ggatttactg gaactggccg agacgcattt aaagcgtttg ggagattcct gatcgatgtc
      301 gggcggctcg agtgtataag taaaccatta gtgatataag gagagaatta tgaacttgcg
      361 acgtaaagtg atacaagtca cgggaatcct gatgttgata tctgctgttg tcatgccgtt
      421 ctcgtccacc gggttggctg acacatcgac attaaccggg caccaaagag aaacccttca
      481 gatgtacgcg caggatacct ggcaatcttt tctcgccatg ctcgaccctg acaccgggct
      541 gcccgccgat aatgtcagcg tcgacggcga gcggtcaggc ttcacctcac ctaccaacat
      601 cggtatgtat gtctggagca cattggcggc gcgcgatttg caaatcatcc aacctttaga
      661 ggcacgccag cgaataggaa ctgtgctcaa taggttggag atgatggagc gccataccga
      721 tagcggtatg tactacaact ggtatgatcc agccaccggc gctaagctta ccatatggcc
      781 tcccagcggt gatccagtgt acccattctt atcgagcgta gacaatggct ggctcgcttc
      841 agctctgatc atgatcacca acgctgttcc acagttacgc gaccaggcgc aggtcatcct
      901 gaatagcatg gacttcagct gctactacga ccctaatgcg cggggctcag attatggcgc
      961 tggcctgctg cgcggtggat tctggacggt ggaggacgcg cctccctggg ctactgcccc
     1021 actggacaac tactgcgata aaggaccgga tgtgatctac accggccacc attatggaac
     1081 atttaacacc gagccccgca tcgccagcta cataggcatc gctctgggac agatcccacc
     1141 ggagcattac ttcgccggat ggcgcacatt cccccccacc tgcgattgga gctggccaga
     1201 gatggcgccc cagggtgaga cgcacaccta cctgggcata gaagtctacg agggatctta
     1261 tcaataccgc gggatgaaca tcgtacccag ttggggcggc agtatgttcg aagcgctgat
     1321 ggtcccactg gtggttccag aggcggaatg gggccagaaa agctggggaa tcaaccaccc
     1381 gctctacgtg caggcgcaga ttgaacacgg cctggaagaa gcccagtacg gctactgggg
     1441 attctcgcca tccaataacc cggctggtgg ctaccgtgag tttggtgtgg acgcgctggg
     1501 catggataca ggtggataca cttccgacct ggagcggacc tcagtcgatt acggctttgc
     1561 cgatccctct ggaatcggat actgccccgg ccgagagccg cagcccctgc cagaaaccta
     1621 tggccagggc gtggtgaccc cgcatgcctc tttcctggcg cttgaattcg caccggaggc
     1681 tactctggag aacctggata acctgcgccg ggattttgaa atttattcct ggggcggatt
     1741 tttcgacgcc gtcaacgtaa gcaacggcga cctctccaca tattacctgg cgctcgacca
     1801 gggtatgatt atggcgtcca tcagcaatgc tttgcgaaat gatcgtctgc aacattattt
     1861 cacccacggc gctgttgaag agttcatgag accgttgatg gaaatggagg aattctccgc
     1921 gcattagcgg cggcgctggc cttccaggag acctgggcag gggtatgttt ccggttttca
     1981 ggatccgcac cggattccct gcgactggcg cacgcaggta aacctataaa tgcaagcgcc
     2041 aggtactgat gtatctggcg cttgctgctg ttcgggagga aaacctttgc cccaatttca
     2101 aacagaatat ggttattttt gcgatgatgg gcaggaatat atcatcaccc gcccggatac
     2161 acctgtgccg tggatcaatg tgatttcaaa tcagaactat gggctggttt tatctcagtc
     2221 cgggggtgga tacagctggt tgagccatgc cagcctcaat cgtatcaccc gatgggagca
     2281 ggatctgatc cgagatgagt gggggaaatt catttacttg cgtgacatgg acagcggtga
     2341 gttttggtcg ccggcatggc aacctgctgg ccgaaaactg gatgaatact gcgtccgtca
     2401 cgggcgaggt tattcgataa ttgagtctcg ctgcgcagag atcgaaaccc ggctgaccta
     2461 ttttgttcct cgctttgatc cctgtgaaat atggcagctc aagatcaata atcgaagttc
     2521 ggtctcgcgc aggttccagg ttttcacata tttcgagtgg cttcttggcg cagcgccaga
     2581 ttggcatcgc gagttccacc gcttatttgt gcgcacctgg tacaaccaac aaaagggtgc
     2641 cttgctggca tccaaggtgc tttgggatct tcccggtgag actggcccgc attggaatcg
     2701 agactgggcc tatgtggcat tccactcttc ctcgattcat cccagtggat atgatgctta
     2761 taaggacgca ttcctgggtc gtaatcaaag cctggctgcg cccgatgcgc ttaacgaggg
     2821 gcggtccctg aatactacgg gtagctgggg agatccgatt ggtagcttgc aggtggagat
     2881 tagactggag gctgatcagg agctggagct cattttcaca ctgggcgcca ccgagactga
     2941 gtcccaggcg cttgatctgg cagagcagta caaaaatcca cacgcagttc aagtggcatt
     3001 tgatgaggta aagagcttct ggcagggtat tgggaaaggt ttaatcgtcg agacgccgga
     3061 tcacgccctc aacctgatgg ctaatacctg gctgccctat caggtgattt ctggacgctt
     3121 atggggacgg acagcctact tccagactgg cggtgccttc ggttttcgtg accagctgca
     3181 ggatagttta ttatggcttc tccttggcca acctgagcaa accctcgccc agatccggtt
     3241 acacgcctcc caccaatata cagatgggat cgttttgcat tggtggcatc cattggcaga
     3301 ggtgggatta cgttcagagt acagtgatga cctgttatgg ctgccatttg ttgtcctgca
     3361 ttatatttat gaaactggtg acctcacttg tcttgaagaa atgatcccct tcttcgatgg
     3421 aggatcagcc actttgcacg agcactgcaa acgtgctttt acggtagctc ttgagcgcag
     3481 aagcccgcat ggactccctt taatcctgaa aggggattgg aatgatggtc tgaatgcagt
     3541 aggagcgggg gggaagggag agagcatctg gatggcgctc tttctttatt atttgctcac
     3601 cagatggtcg gagcttcctg acctcgatga agaaatgtgt ctgcggtata aagctgaagt
     3661 caaagcgcta cgcgattcga cagagattta tggctgggac ggcgcgtggt atcggcgcgc
     3721 tacgaccgac gatggccagc acatcggctc agcccaaaac aacgaaggcc agatatttct
     3781 caacacgcaa acctgggccg tcttaagtgg actggcgacg ccggatcgcg ctggacaggc
     3841 gattctttcg gcgcgccagc atctatatac ggcttatgga cctctccttt tagcgccagc
     3901 ctattcccag cccgatcctg agatcggcta cctcacccgc tatgccccgg gagtgcgcga
     3961 gaatggtggc gtgtacgtcc atgcggcatg ctgggcagtc atggctgaga gaaaagcgca
     4021 tggcgtgcag gccgcttacg atctctggcg tagtttttgc ccgccggttc gtggtatgga
     4081 accggaagcc tacacagccg aaccctacgt gatgccgggt aatgttgatg gtccggattc
     4141 tcaatttcct gggcgcggcg gctggacatg gtacaccgga tcggcggcgt ggtactaccg
     4201 ggcattgata gaaggagtac ttggggtcga ggcaacgaaa gaaggattgc gcgttcaggc
     4261 tgatctgcca gatggctggg atcgctacca cgtcaagcgc ctctttcgcg gcgcgactta
     4321 cgatatccac atccgacgag caaattccgg agaacagact ggttgtacag tcgacgatcg
     4381 accctggcag ggggaaacac tgccaatcct ggagccaggg acggtgcatc tgattaagtt
     4441 cacttgccgg taggaggcga ctatcaatga tccagtccat gcctttgcag ccttacactc
     4501 ccggcacata ttacaaccac ctcatcttcg acaacagtct atcggatggc ggctattttt
     4561 tcagtcacgc agttgcgatt gcacccagcc agctggaagc gatcgactgc aagataccgg
     4621 ttgccgcgga tcgcttcctc agccctccca acagtctacg cctttcgtgg ttgtcgcaaa
     4681 ccggtggaga ctggctggcg gagttataca gcgaaacctg gctcaacaag acacgcttgc
     4741 tttctggcga taccctttca ttctggggct actcggaaga agagatccct gtgcgtctac
     4801 tcccaatgat tcgcctaaaa atcaaggatg ggccacttac ttcaccgtta caaatgaccg
     4861 gcatagtgcc agacttgcca gccaggcact ggcagcagat caatattcca cttgaagcct
     4921 tcccgaatga aactttcaat gtcgatttca gccggatcga aaaaatcatc ttcacccaga
     4981 gcctcgatga caactttccc cacaccttgt atattgatga ggtcaagatc tctgactctg
     5041 ctatggttgg aatgggtcga ccgccgagtg gactttctgc ggtggcttat gattgccatg
     5101 tcgatctgag ttggcgtgca gtgaatgatc cagcggtaat gtattatcaa atttaccgct
     5161 cgaacaatgg gcaggacttc ctgccggttg gaattcaaaa ccctaccttt aaccgttatg
     5221 tagactacgt cgggcaatcg cagagaaagc tcttctatcg aataacctct gtcagctcca
     5281 attattccga gtctcccgct tctcaaatag tttcagggac aacccatccg atgactgacg
     5341 acgagctgtt gagcatggta caggaagcct gcttccgcta ttattgggaa aatgctcatc
     5401 caaatgcggg tctggcattg gagaatgtgc ccggtgacga gaatctcatt gcattaggtg
     5461 cgtccggctt tgggatcatg gcattgattg tcgcggtctg gcgtggcttt gtggcaaggg
     5521 aaacagctat cgagagactg ctcgtgatcc tctcgtttct agaaatcgcc gaccgctttc
     5581 acggcgtttg gccgcacttc ctggatgggc gcacaggaaa agtaattccc gtcttcggga
     5641 agtatgacaa cgggggtgac ctggtagaaa cggcattcat gatgcaagga ttaatggcag
     5701 cacgccagtt cttcgcaaac gaagaagagg gagaaaagca gatccgttcc cggatcacca
     5761 gcctgtggga aaccgtcgag tgggattggt accgccaatc agcagatagc gagttcctct
     5821 actggcattg gtcgccagat tatggctggc atatcgggca tcccttgacc ggatggaacg
     5881 aaaccctgat tgcttatttg cttgctattg cttctcccac gcatccggtg ccagcggcaa
     5941 tgtattattc cggttgggcc tcatcatccg aaaccgccca gcgctaccgc caggcgtggg
     6001 gacagacaac tcagggagat ctctaccgaa acaggaatgt ttactatgga atccaactcc
     6061 cggttggtgt cggctccggc ggtcccctct tcttcaccca ttattccttc ctgggtttcg
     6121 atcctcgcaa taaacgggac aggtttggcg attactttga aagtaatcgg gcaattgtac
     6181 tcattaatcg tgcttactgt atcgaaaatc ctggcaaata tcagggttat ggtgaaaact
     6241 tctggggctt gacagccagc cacgatcata ccggttacct ggcacacgaa cctagcccga
     6301 gggcggacaa cggaacgatc actcctaccg cagccttatc ctcttttccc tatacaccgg
     6361 agggttcgat ggcggcttta aagcatttct actacgatct gggcgacaag atgtggggga
     6421 tctatggctt cagagatgca tataatccga cagtcaatta cgtttcaaac attttcatgg
     6481 gactaaacca ggcaccgatc accgtaatga tcgagaatta tcgcagcggt ctgttatgga
     6541 atcttttcat gtctaacccg gaaatcacaa gcatggtgaa acgaatcggg ctcgttaagt
     6601 aaccaaaccg taaatattcc tttcgagccc tgatttcctc tggcaacttt gaataaggtt
     6661 ggatccaccg gcactacata acagagtccg ttgagcaggt cagaccagca gtagacggcg
     6721 gagtcttatg catccagcaa cagatgcaga catcaccgcc aggaactgca cccaacggca
     6781 ccacatctgc accatttaag acaaaaaatc cgctgatcag cggattttac ccccacatct
     6841 aggtgacccc ggggcggctc gaacgcccaa ccaattgatt aagagtcaac tgctctacca
     6901 ttgagctacg gggccttgcg tgccgtatta taccccaaag gaatttgttg tcaacaaaaa
     6961 tgacaggtgg caggttctga ttctagttat aataaccccg tttatgggtg attttgagcc
     7021 cccgaagctt gcttgccaga ctttattcat cgagacattc attcaatgag ccccgggctg
     7081 atcgatactg taacagaaaa agataccctg cttgtcgttg aggacaacga tgcactgcgg
     7141 gaaggtttac gcgagatgct cagcctggaa gggttcgcgg tgctggcagc tgccaatggg
     7201 cgcgccgcgc tcgagcagat gagcaccctg acccccgatc tgatcgtctc cgatatttcc
     7261 atgcccgaga tggatgggat tgcttttttc aaggcggttc gggagcgccc cgactggctg
     7321 acaatcccct tcctcttcct ttccgcgcgc ggggaaaagc aggatattct gcgtggaaaa
     7381 aacctgggcg ctgaagacta cctggtcaag ccgcttactc gcgatgaact ggtaactgca
     7441 gttcgctccc ggctggaacg cttcgagcag ctccggttag cgcagctcca gcgagcctat
     7501 gaggccagcc tcaccgccct ggcaaatgcc attgatttgc gagatcgcta tactcgcggc
     7561 cacgtggaac gtgtcactgc gtactgcctg gcgatggcga gcaagttgcg ttggcccgaa
     7621 atgaacctgg atcacctgag gtttggggcg atcctgcatg atattggtaa aatccatatc
     7681 ccagagaata tattattcaa aaagacagcc g
//
LOCUS       DATMXK010000082         2012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_18704, whole genome shotgun sequence.
ACCESSION   DATMXK010000082 DATMXK010000000
VERSION     DATMXK010000082.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2012
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18704"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..654
                     /locus_tag="VN363_01570"
     CDS             40..654
                     /locus_tag="VN363_01570"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWQ83221.1"
                     /translation="MNSDPADSQVTTVDVGERLRILREERGLSMRSLARDSGLSTNAL
                     SMIERGRTSPSVSTLVKLAGALGAPITAFFRTEPERSNIVFRKASERTKITIPAGIYE
                     GLGGDAFNGLMEPFILTIDPGSSSGDHDMIHTGQEFALCLEGVIEYALETQLYRLEAG
                     DSLIFAANLRHSWHNPGPGYAKMAVVLSAFELGESPSEFHLAAA"
     gene            776..>2012
                     /gene="aspS"
                     /locus_tag="VN363_01575"
     CDS             776..>2012
                     /gene="aspS"
                     /locus_tag="VN363_01575"
                     /EC_number="6.1.1.12"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate--tRNA ligase"
                     /protein_id="HWQ83222.1"
                     /translation="MYKTHTCGELRAGHAGEAVTLAGWVHRRRDHGGVTFVDLRDRFG
                     LVQVVADPVNAPEAHEALLAVRNEWVIQVHGTVRRRPAGSENPNLPTGEVEVIASTVQ
                     VLNPAKTPPFLINKDEEVDENSRLKYRYLDLRREKMRHNLELRHRVVKFIRDYLDTRG
                     FLEVETPILFKTTPEGARDYLVPSRVHPGEFFALPQSPQQLKQMLMVGGIERYFQIAR
                     CFRDEDQRGDRQPEFTQLDLEMSFVEREDIMQLIEGMFTEMVAKVVPHKRLQSSPWPR
                     LKYQEAMDRFGKDNPDIRFGLELKDLTDLAPGSGFKVFENAAASGGQVRALNAKGLGG
                     YTRKQLDELTEFVKQFGAKGLAYLAVEADGNHRSTFSKFLTPEWLAKLFERTEAEPGD
                     LLLVVADQPAVVYESLGRLR"
BASE COUNT          435 a          562 c          641 g          374 t
ORIGIN      
        1 atactggact gtaaagatcg gcatcacttg ggaggcaata tgaattcaga tccagcagac
       61 agtcaggtaa caacggtcga tgttggcgag cggctgcgga tcctgcgcga agaacgcggg
      121 ttgtcgatgc gctccctggc gcgcgacagc gggctctcaa ccaacgctct gagcatgatt
      181 gaacgtgggc ggacctcgcc ctcggtaagt accctcgtca agctggcggg agctctgggc
      241 gcgccaatca cggctttctt ccgcaccgag cctgagcgct cgaatattgt atttcgtaaa
      301 gcttccgaac ggacgaaaat aaccattccc gccgggatct atgaaggtct ggggggagat
      361 gccttcaacg gtttgatgga gccgttcatt ctgacgattg acccgggcag ctcgagcggg
      421 gaccatgaca tgatccatac cgggcaggaa tttgccctgt gcctggaagg cgtgatcgaa
      481 tacgccctgg aaacgcagct ttaccggctc gaggcgggcg acagcctgat ctttgcagcc
      541 aatctgcgcc acagctggca caacccggga ccgggttacg caaagatggc ggtcgtactg
      601 tctgctttcg agctggggga gagcccgagc gagttccatc tcgcggcagc ctgaaataag
      661 caaaaagaac gtgctataat tcgagcctac cgcacgcgga acgattctgt gagtctggaa
      721 aaatctatcg cctgaggcgg attgctgagg gcgattttta ttatggagga gaggcatgta
      781 taagacgcac acctgtgggg agctgcgcgc cggtcacgct ggagaggcag tgacgctggc
      841 gggctgggtg caccgccgcc gcgatcacgg cggggtgacc tttgtagacc tgcgtgatcg
      901 atttgggctg gtgcaggtgg tggccgaccc ggtgaacgct ccggaagcgc atgaggcact
      961 gctcgccgta cgcaatgaat gggtgatcca ggtgcatggg acggtacgcc gccgccccgc
     1021 tggctctgag aaccccaatc tgccgacagg cgaggttgaa gtcattgcca gcacggtcca
     1081 ggtgctgaac cccgccaaaa ccccaccttt cctgatcaac aaagacgagg aagtggacga
     1141 aaactcccgc ttgaaatacc gctacctgga tctgcgccga gaaaaaatgc gccacaacct
     1201 ggagctgcgc caccgggtgg tgaaattcat ccgcgattat ctggacacgc ggggattcct
     1261 ggaagtggaa accccgatcc tgttcaagac gactcccgaa ggagcgcgtg actacctggt
     1321 gccatcccgg gttcatcccg gcgaattctt cgccctgccg cagtcgccgc agcagctcaa
     1381 gcagatgctg atggtgggcg ggattgaacg ctacttccag attgcgcgct gcttccgcga
     1441 cgaagaccag cgcggcgacc gccagcctga attcacccag ctcgacctgg agatgtcctt
     1501 cgtggagcgg gaagatatca tgcagctgat cgagggcatg ttcaccgaga tggtggcaaa
     1561 agtggtgccg cacaagcgcc tgcagagcag cccatggccg cgcctgaaat accaggaggc
     1621 gatggaccgc tttggcaagg acaacccgga tatccgcttt ggattggagc tgaaggacct
     1681 gaccgacctg gcgccgggga gtggttttaa agtattcgag aacgcggcag cctccggcgg
     1741 gcaggtgcgc gctttgaatg ccaaaggcct gggcggctat acccgcaagc agctcgatga
     1801 gctgaccgaa tttgtcaaac agtttggcgc caaagggctg gcttacctgg cggtggaagc
     1861 ggatggcaac caccgctcga cattcagcaa atttctgacc ccggaatggc tggcgaagct
     1921 ctttgagcgc acggaagccg agccgggcga cctgctgctg gtagttgcag accagccggc
     1981 agtggtctat gaatcgctgg gccggctgcg gg
//
LOCUS       DATMXK010000083         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_53671, whole genome shotgun sequence.
ACCESSION   DATMXK010000083 DATMXK010000000
VERSION     DATMXK010000083.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53671"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2508)
                     /gene="uvrA"
                     /locus_tag="VN363_01580"
     CDS             complement(<1..>2508)
                     /gene="uvrA"
                     /locus_tag="VN363_01580"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560703.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrA"
                     /protein_id="HWQ83223.1"
                     /translation="HCPICGREVFRQSAQEVVESVEALPEGSRLLLLAPLVRGRKGTH
                     QAVLDEIRKAGFVRARVDGQVYELEEDIQMDRYKIHNIEAVVDRLVIRHQEEDPDFQA
                     SRSRLTDSVETALKFGDGYLIIQDLTDPQEPRDLYFSEHLACPEHGVSLPEIEPRTFS
                     FNTPHGACPDCQGLGGKLEIDPDLLIPDNDLSLNEGAIIVSEWSGPRDEGGYYWQTLE
                     AVAHDYHIDLNAPVSKLTPQQLDLVMYGTQGREVTIHYSNRDGRKATFRQPFEGVIRN
                     LERRYNETSSEYMREKLSEFMSERICPTCHGARLRPEALAVTVDDQNIIAVTTWPVLK
                     TLEWVERLASPDNVLTSRQQAIAERILKEIHARLGFLVDVGLDYLTLQRSAASLSGGE
                     AQRIRLATQIGSRLMGVLYVLDEPSIGLHPRDNARLLRTLRGLVDLGNTVLVVEHDNE
                     TILEADWILDLGPGAGEHGGKVVAEGTVEQILAHPDSLTGAYLSGRKRVPIPEKRRNG
                     NRKSLKVVGAQENNLKNIDVTIPLGRLVCITGVSGSGKSTLMVDILYKALAHALHGAH
                     SQPGAHERIEGLEHIDKIINIDQSPIGRTPRSNPATYTGLFDTIRALFAELPESKVRG
                     YKPGRFSFNVHGGRCEACQGQGQLRIEMQFLPDVYVPCDVCHGARFNRETLQVRFKDK
                     NIAEVLEMTVEEGLEYFSSFPRMLNRLQTLNEVGLGYIRLGQPATTLSGGEAQRVKLS
                     KELSRRSTGRTVYVLDEPSVGLHAADVHKLIEVLQRLVDVGNSVLIIEHNLDIIKVAD
                     YVIDLGPEGGDRGGYVIGAGTPEEICAMDESYTGQYL"
BASE COUNT          537 a          766 c          649 g          556 t
ORIGIN      
        1 ctcagatact gccctgtata tgattcatcc atcgcgcaga tttcttccgg agtgcctgcg
       61 ccgatcacat accccccccg gtcgccccct tcaggtccca ggtcgatcac gtagtcagcc
      121 actttgataa tatccaggtt atgctcgatg attaacacac tgtttcccac atccaccaga
      181 cgctgcagaa cctcgatcag cttgtgcaca tccgcggcat gcaggccaac tgacggctca
      241 tccaggacat acactgttct gccggtcgag cgccgcgaca gctccttcga aagcttcacc
      301 cgctgcgctt ctccgcctga gagagtagtc gccggctgtc ccagccggat atatcccaga
      361 ccaacttcgt tcaaagtttg cagacgattg agcatccggg ggaaagagct gaaatattcc
      421 aagccttcct ccacggtcat ttccagcacc tcagcaatat ttttatcctt gaagcgcacc
      481 tgcaaagttt cacgattaaa gcgggcgcca tgacagacat cgcaaggcac gtacacatcc
      541 ggcaagaact gcatttcgat ccgcagctgc ccctgtccct ggcaggcttc acagcgtcca
      601 ccatggacat tgaacgaaaa gcgccccggc ttatacccgc gcaccttgct ctctggcagc
      661 tcagcaaaga gggcccggat agtatcgaac aaaccagtat atgtagctgg atttgaacgg
      721 ggcgtgcgcc caatcggcga ctggtcgata ttgatgatct tatcgatatg ctccagcccc
      781 tcaatccgct catgcgcgcc aggctgggag tgggcgccat gcagggcgtg ggcgagggct
      841 ttatacagaa tatccaccat caaagtcgat ttgcctgagc cggaaacccc ggtgatacac
      901 accagacgtc ccaacggaat ggttacatcg atattcttca ggttattttc ctgcgcgcca
      961 acaactttca aactttttct gttgccgttg cgtcgttttt ctgggatggg gacccgtttt
     1021 cgcccagaga gatatgctcc cgtgagcgag tctggatggg cgaggatctg ttctacggta
     1081 ccttctgcga ccactttccc gccatgctca ccagcgcccg gtcctaaatc caggatccag
     1141 tcagcctcca ggatggtttc attatcatgt tccaccacca ggactgtgtt tcccagatcg
     1201 accagccctc tcaaagtgcg cagcaagcgg gcattatcgc gaggatgtaa accgattgag
     1261 ggctcatcca gcacatagag cacgcccatc aggcgcgagc cgatctgggt tgccagacgg
     1321 atacgctgcg cctcaccacc agagagtgat gctgctgagc gctgcaaggt gagataatcc
     1381 aggcctacat ctaccaggaa gcccagccgg gcgtggattt ctttgagaat ccgctctgcg
     1441 atcgcctgct ggcgactggt aaggacattg tccgggctcg ccaggcgctc gacccattcc
     1501 agggttttca aaaccggcca ggtagtcacc gcgatgatat tttgatcgtc gaccgtcact
     1561 gccagcgctt ctggacgcag gcgcgctccg tgacaagtcg ggcagatgcg ctcactcata
     1621 aactcagaga gcttttcgcg catatattca gagctggtct cgttgtagcg ccgctccagg
     1681 ttgcggatca ccccctcaaa tggctgacga aaagttgcct tacgcccatc ccggtttgag
     1741 taatggatgg tgacttctct gccctgggta ccgtacatga ccaggtccaa ctgctgtggg
     1801 gttaatttgc tcaccggcgc attcaagtcg atgtgatagt catgggccac ggcctccagg
     1861 gtttgccagt aatacccgcc ctcatcgcgt ggcccgctcc actcggaaac gataatcgct
     1921 ccctcattca gtgaaaggtc gttatccgga atgagcaaat cggggtctat ctccagtttg
     1981 ccgcccagtc cctgacaatc tgggcaagcg ccgtgtgggg tattgaagga aaaagtgcgc
     2041 ggctcgatct ccggcaggct gacgccgtgc tccggacatg ccagatgctc tgagaaatac
     2101 aggtcgcgcg gttcctgtgg atcggtcaga tcttgaataa tcaaatagcc atcgccaaat
     2161 ttcagcgccg tttcaaccga atcggtcaga cgcgagcgag acgcctggaa gtcggggtct
     2221 tcctcctggt gacgaatgac cagcctgtcg accactgctt cgatattgtg gatcttgtag
     2281 cggtccatct ggatatcttc ttctaactcg tacacctgcc cgtccaccct ggcgcggacg
     2341 aagcctgcct tgcggatctc atccagaact gcctggtgag tgcccttacg cccgcgcacc
     2401 agcggcgcca ggagcaaaag ccgcgaccct tcgggcagcg cttcgaccga ttcgacgact
     2461 tcctgcgctg actggcgaaa aacttcccgc ccacaaatgg ggcagtgc
//
LOCUS       DATMXK010000084         5379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_67871, whole genome shotgun sequence.
ACCESSION   DATMXK010000084 DATMXK010000000
VERSION     DATMXK010000084.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67871"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..891)
                     /locus_tag="VN363_01585"
     CDS             complement(<1..891)
                     /locus_tag="VN363_01585"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWQ83224.1"
                     /translation="MPDHTRTVPDFAAARSYVLDRLERELSPRLVYHSVVHTRDDILP
                     ALERLAEREGITDEHELVVLRTAGLYHDIGFTETYAGHEIASIRIAAETLPGFGYSQA
                     QIEQICHLIEATEMPQNPDSRLAKLLADADLDILGREDFLIRNRDLYRELADMGKDFT
                     LTEWCSSQLTLLQTHRYFTRAATELRQAGKEQNMARLQDQLAGSSNPDSPATGKASTG
                     EKIEDILARIPLFTTLPKEELAHLGKSLQLSEYPVPTLLFREGDHGDFMYLVIEGFIE
                     IFKALGTPDERRIGLRGSGEY"
     gene            complement(921..>5379)
                     /locus_tag="VN363_01590"
     CDS             complement(921..>5379)
                     /locus_tag="VN363_01590"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="tandem-95 repeat protein"
                     /protein_id="HWQ83225.1"
                     /translation="LTDFIDLPGQSRWYKFNVQPGSKLVISLTGLAANYDLTLYKDIA
                     QAFKTLNSPADLLRLDAEFAPEAFSPEAFSPEAFSPEAFSPEAFSPEAFSPEAFSPEA
                     FSPEAFSPEAFSPEAFSPEAFSADAYSPEAFSPEAFSPEAFSPEAFSPEAFSPEAFSS
                     AQSRSLIGISAFEGVVSEGLQVNTWDSTGDFYVRVRGRNGAFDVEMPFNLSVRQIPGS
                     CTTVSDVLPASTLVPDAGGYHTLILTNLPRMEGSPEEKSLLLNRLQQFALRPEVAGKI
                     IDLSLDDRFTAAEIQARQNLTCPYAMNLAASTIKETVDAFWELNPALEYLVIIGNDEV
                     IPFFRHADHAMLGNEKSFVPPVRDNTSSQTSLRLGYVLSQDDYGASLTISLKDDAFPV
                     PDLGVGRLVETAAEATGMLDAYLITSAGVVGTPASAFVSGYDFLDDAARAVRDQLSAG
                     MSLDGSVQVNTLISPRQLSPTDPSAWTADQLRAVLLTERHDLIFLAGHFSASSALAAD
                     YSTRFFSTELISSEVNLLNSLVFSVGCHSGYNIVNSHNVPGVTREPDWAQAFARKQAT
                     LIAGTGYQYGDTDFIRYSEKLYLEFARQLRVGTGPVSIGQALVRAKQAYLASTPYLRG
                     IDEKAVLQATIFGLPMLSYNLPGRLLDSGSEPSIVSGTQVVTGNPGQTLGLETYDLNV
                     QPQLAINTVELTDVQNLGVTNQAVYLSGSHEVISKPNEPVLPLAQYNVSVPGKALRGI
                     GFRAGSYADMLNILPFTGAPSTEIRGVHSPFQSNFFYPVQLWRPNYFSALDGGNTLLN
                     VFPAQYISSSPTSITGTLRRFDQLDLRLFYSSNIESYTIGGFVSTPALAAAPSIINVM
                     SQIENGLVRLQSRVIGDPAAGIQQVWVTFTGLSAPFYGSWQSLDLVQNSYDSSLWEAE
                     LPLNNFPAGDIRFVVQAVSGVGLVSMDTNQGSYYIPGVDPEAGEASSLALVPNAGDNQ
                     IASSGRYTEQVTFSARLTSNGAPLAGKAVAFILGPQSRLGITDSNGVAVVTLSIYGLA
                     GDTEVRTSFAGDGSYQASFAAQPFTIQRQISLITVDPQPASGYGEEAGLLTARLLDVS
                     GRSLSEETVFFALRSGTIQYYEAVITDYAGRASLGSLPLPPGSYQVSVYFSGQIPLTE
                     TINLTDDRYEPAVYLDGSLTLLNHLPLANIDSYPVEEDGLLAIAAPGVLGNDSDQDGA
                     QLTALLASGPNHGTLNLNPDGSFSYAPDLNYNGSDDFSYYANDGQDNSLVQVSIQVTA
                     VNDAPQAYPDTYAALEDTDLTIPAPGLLGNDQDVDGDALSAFLVSLPSAGTVTLNQNG
                     SFTYHPEQNYNGRVSFLYQANDGQTSSEPALVTIEVGAVNDPPSCITALPNPDSLWSP
                     NGLLIPVSLINISDPEGNPFNITISSVFQDEPVGREADAFLQGPSALLRSERDGNGNG
                     RVYHIYFSAVDLFGASCTGHTTIGVVPHDQSGNVAAIDGGPLYDSTLPQ"
BASE COUNT         1262 a         1416 c         1534 g         1167 t
ORIGIN      
        1 atactccccg gagccgcgca aaccgatccg gcgttcatcc ggagttccga gagccttgaa
       61 gatttcgata aacccctcga tcaccaggta catgaaatcc ccatggtcgc cttcacggaa
      121 aaggagggta ggaactggat actcactcaa ctggagagat ttacccaggt gagccagttc
      181 ttccttggga agggttgtaa ataatgggat ccgcgccaat atatcttcaa ttttctcgcc
      241 agtgctcgcc tttccggttg cgggagaatc gggatttgag ctgccagcga gctgatcctg
      301 cagcctggcc atattctgct cctttccagc ctggcgcagc tcggttgcag cccgggtgaa
      361 atagcggtgc gtctgcagca gcgtaagctg tgagctgcac cactcagtaa gcgtaaaatc
      421 cttacccata tccgccagct cccggtagag gtcgcgattg cggatgagga aatcttctcg
      481 ccccaaaatg tccaggtcgg cgtcagccag cagcttggcc agccgggagt cgggattttg
      541 cggcatctcc gtcgcttcga ttaaatgaca gatctgctca atctgagcct gactgtaccc
      601 aaatcctggt aaagtctcgg ctgcgatccg gatactggcg atttcgtgtc ccgcataggt
      661 ttcagtgaat ccgatatcgt gatagaggcc ggctgtgcgc agcacaacca gctcgtgctc
      721 atccgttatt ccttccctct cagccagacg ctccagggct ggaaggatgt catctcgggt
      781 atggacaacc gagtgataga caagtctggg agacagttcg cgttccagtc tgtcgagcac
      841 gtagcttcgg gctgctgcga agtcagggac ggtcctggtg tgatctggca tagatgtgaa
      901 gggttgctcc atccggaaca ctattgtggc agggttgagt cgtagagagg cccaccatcg
      961 atcgcagcaa catttccact ctgatcgtga gggaccacac caattgttgt atgcccggtg
     1021 caggaagcgc caaacaagtc caccgcacta aaataaatgt ggtaaacccg cccatttcca
     1081 ttgccatcgc gctccgagcg caagagcgca cttgggcctt gaaggaaagc atcggcctct
     1141 cgacctacag gctcgtcctg gaatacactg ctgatcgtaa tattgaaggg attcccttct
     1201 ggatcagaaa tattgatcag gctgaccgga atgagcagac cattgggtga ccagagactg
     1261 tcggggttcg gcagcgcagt gatgcagctg ggtggatcat tcaccgcgcc tacctcgatc
     1321 gtaaccaggg caggctcgga ggaggtctgc ccatcgttcg cctgatacaa gaacgatacc
     1381 ctcccgttgt aattctgctc ggggtggtag gtgaaagatc cattctggtt aagggttacg
     1441 gtgcctgcag aaggtagact gaccagaaac gccgagaggg catccccatc cacatcctga
     1501 tcattgccca gcaacccggg agcaggtatc gtcaaatctg tgtcttcaag agcggcgtaa
     1561 gtatccggat acgcctgggg agcgtcatta actgcggtaa cctggatgga tacctggacc
     1621 aggctgttgt cctgcccgtc gttggcgtaa taactgaaat cgtcgctccc gttatagttc
     1681 aaatctggag cgtagctgaa agagccatcc ggattgaggt ttagcgtgcc atgattgggt
     1741 cctgaagcca gtaaggcagt aagctgagcg ccgtcctgat cggagtcgtt acccaggaca
     1801 cctggtgccg cgattgccag cagcccgtct tcttccaccg gatatgaatc gatgttggcg
     1861 agaggcaaat ggttcagcag agtcagtgag ccatccagat agactgctgg ctcgtagcga
     1921 tcatcagtga ggtttattgt ctcagtgagg gggatctgcc cgctgaaata aacggacacc
     1981 tggtagctgc cagggggtaa tggcagtgaa cccaaggaag cccgcccggc ataatccgtg
     2041 attaccgcct catagtactg tatcgttcca gatcgcaggg caaagaacac agtctcttca
     2101 ctcagcgagc gaccgctgac atcgagcagt cgggctgtca gcagcccagc ctcctctcca
     2161 tatccgctgg cgggctgcgg gtctaccgtg ataaggctga tttgccgctg gatggtaaat
     2221 ggctgcgcag caaatgaagc ctgataactg ccatcaccag cgaatgaagt gcgtacttct
     2281 gtatcgccgg ctaatccata aatcgaaaga gtgaccacag ccacgccatt gctatctgta
     2341 attcccagcc ggctctgtgg gccgagaatg aacgcaaccg ccttacccgc cagcggtgca
     2401 ccgttgctgg tcaatcgagc gctgaatgtt acctgttccg tataacgccc cgacgaagcg
     2461 atctggttat cacctgcatt gggtacgagc gccaggctgc tggcctcacc tgcctctgga
     2521 tctacacctg ggatgtagta actgccttga ttggtatcca tactgaccaa tcccacgcca
     2581 ctcacagcct ggacgacaaa ccggatatcc ccggccggga aattgtttaa gggtagttca
     2641 gcttcccaca aggatgagtc gtaggaattc tggaccaaat ccagcgactg ccagcttcca
     2701 tagaatggag cgctcaggcc agtaaacgtg acccatacct gctggatgcc tgcggccgga
     2761 tcgccaatca cgcgcgactg gaggcgcacc aatccatttt cgatttgtga cattacgttg
     2821 atgatggatg gcgctgcagc cagggcaggc gtgctgacaa atccaccgat tgtgtaagat
     2881 tcgatattgc tgctgtaaaa caagcgcagg tcgagctggt caaagcggcg aagtgttccg
     2941 gttatggaag taggtgatga agaaatatat tgggcaggaa atacattcaa caacgtatta
     3001 ccgccgtcca gcgcgctgaa gtagttcggc cgccagagtt gaacagggta gaagaagttg
     3061 gattggaatg gggaatggac gccccggatc tctgttgatg gagcgccggt aaaaggaagg
     3121 atatttagca tatccgcgta agaaccagcc cgaaaaccaa ttccacgtaa tgccttccct
     3181 ggaacactga cattatattg agccagcggc aagactggct cattcggctt ggagatgact
     3241 tcatgcgacc cggagagata aacagcctgg ttagttacac ctagattctg aacatcggtt
     3301 agctccacgg tgttaatcgc caattgtggc tggacattca ggtcataagt ttccaaaccc
     3361 agggtttgcc cggggttacc cgttaccacc tgcgtaccgg atacgatgga tggctcactg
     3421 cccgaatcga gcagccggcc gggcagattg taagagagca tgggcaggcc aaagatggtg
     3481 gcctgtaaaa cagctttttc atcgatgccg cgcaggtaag gagtgcttgc cagataagcc
     3541 tgcttggccc gcacaagtgc ctgaccgatc gagaccggac cggttcccac ccgaagttgg
     3601 cgagcgaatt ccaggtagag tttctcgctg tagcgaatga aatccgtatc accatactgg
     3661 taaccggttc ctgcgatcag agtagcctgt ttgcgggcga atgcctgcgc ccagtctggc
     3721 tcgcgagtta ccccgggcac gttatgcgaa ttgacaatgt tatagccaga gtggcagccc
     3781 acactgaata ccagcgaatt gagcagatta acctcggagg agatgagctc agtgctgaaa
     3841 aagcgggtgg agtaatccgc tgccagagcg ctgctggcgc tgaaatgacc tgccaggaag
     3901 atcaggtcgt ggcgttcagt caacaataca gctcgcagct ggtctgccgt ccacgctgat
     3961 ggatcggttg gtgatagctg gcgcgggctg atcagggtat taacctggac actgccatcc
     4021 aggctcatcc cggcggatag ctggtctcgc actgcccggg cagcgtcgtc tagaaaatca
     4081 tagccactga cgaaagcaga ggcaggtgta ccgactacac cagccgaggt aatcaaatat
     4141 gcatccagca tgccggtcgc ctcagcagct gtctccacca gccggcccac gccgagatcg
     4201 ggcactggaa aggcgtcatc tttgagtgaa atcgtgaggc tggctccata atcgtcctgg
     4261 ctgaggacgt aacccagccg gaggctggtc tgggaggagg tgttatcccg caccggagga
     4321 acaaagcttt tttcattccc taacatcgca tgatcagcat gccggaagaa ggggatgact
     4381 tcgtcgtttc cgatgatgac caggtattcc agggcgggat tgagttccca gaaagcatcc
     4441 acggtttcct tgattgtcga ggctgccagg ttcatggcat aagggcaggt caaattctgc
     4501 cgggcctgga tttcagcggc ggtaaaacgg tcatccaggg agagatcaat aattttcccg
     4561 gcaacttcag gccgcagcgc aaactgctgc agccggttga ggagtaaaga tttctcttct
     4621 ggtgagcctt ccatccgcgg caggttggtc agaatgagcg tgtggtaacc gcctgcatcg
     4681 ggtaccaggg ttgaagcagg gagtacatcg cttaccgtgg tgcaggagcc gggtatttgc
     4741 cggacactca gattaaacgg catctccacg tcaaatgcac cattgcgacc tcgcacccgc
     4801 acataaaaat ctcccgtgct gtcccaggtg ttgacctgca gtccttcgct caccactcct
     4861 tcgaaggcgg aaatcccgat cagactgcgt gattgtgcgc tggaaaatgc ctcgggtgag
     4921 aaagcctctg ggctaaacgc ttccggagag aaggcttcgg ggctgaatgc ttctggactg
     4981 aatgcctctg ggctgtatgc gtctgctgag aaagcttcgg gggaaaaggc ttccggtgaa
     5041 aatgcttctg gcgagaaagc ctcgggggag aaggcttctg gactgaatgc ttctggagaa
     5101 aatgcttcag gtgagaaagc ctctggcgag aaggcctcgg gggagaaggc ttccggcgaa
     5161 aaggcttccg ggctgaaggc ctccggggca aactcagcat ccagacgtag cagatcagct
     5221 ggagaattca gggttttaaa tgcctgagcg atatccttat acaaagtcag gtcataattc
     5281 gcagctaaac cagtgagtga gatgaccagc ttgctgccag gctgaacatt gaatttatac
     5341 cagcgggatt gccccggcag gtcgataaaa tccgtcaat
//
LOCUS       DATMXK010000085         3170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_168035, whole genome shotgun sequence.
ACCESSION   DATMXK010000085 DATMXK010000000
VERSION     DATMXK010000085.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168035"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..651)
                     /locus_tag="VN363_01595"
     CDS             complement(16..651)
                     /locus_tag="VN363_01595"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HWQ83226.1"
                     /translation="MEALVQEIYTHVMEGQSEPVKVKVQAALEAGIPAPVILNEGMIA
                     AMAEVGRLFEEGEYFVPEMLIAARAMQAGLALLKPHLADSDVKVAGRVVIGTVKGDLH
                     DIGKNLVAMMLEGAGFEVIDLGADVSPEKFVTAAREHNAQIVAMSALLTTTMPGMKAT
                     IEALNSANLRDQVKIMVGGAPITQDYANLIGADGYSPDASRAVAAAKALLA"
     gene            complement(738..1877)
                     /locus_tag="VN363_01600"
     CDS             complement(738..1877)
                     /locus_tag="VN363_01600"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83227.1"
                     /translation="MNKELTGEIAVPAKSKNNGFRKVLGVIGADNLTLIFALLVLTFL
                     ITVASGWFGMSGGDKFFSWQNLWNSLAQAIVIVGLLAIGETVIIVAGGLDISVGSVAS
                     IGSVVSASVLAGVGTIGALGILPKGSVFIAVLAGILAGMAAGLINGFIITRLRVNPTI
                     ATLGTLAAFAGLAFLLAPDGKPVGVLTQPAFTWLAQGRFLTNLGIPPLAGSKWTGIPV
                     LTVILVAVTILVHILMRYTDFGRAVYAIGGNTIAARLAGINVNKIKVLMYMLSGGIAG
                     LAGVLLTARTTSGNPINGNGLELQAITAVFLGGAATSGGKGTIFGTFLAVILVGVLNN
                     GMNLLGFNTFVQRVALGLLLVAAVAVSQWRQARAERVRTKIATQN"
     gene            complement(1916..>3170)
                     /locus_tag="VN363_01605"
     CDS             complement(1916..>3170)
                     /locus_tag="VN363_01605"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWQ83228.1"
                     /translation="VIYQEPEIVPGIDVAENIWVGELPKKMGLIDRRKLEQLVQESLA
                     EYGFEDTLPIHLLGEELSSAQRQLVEIMRALKSGVRVLALDEPTSSLTDDEVERLFTL
                     VRRLRDEGVAIIYVSHRIKEILKLCDRIAVLRDGNLVAVKPAAELTEREIVRLMVGRK
                     LSEFIHRTPSGTDRVVLRVEDLYSNWHVGVSFHINAGEVIGFAGLIGAGRTELAKVIF
                     GEMPRLRGSIEIDGRETNIRRPDQAISNGVGFAPEDRKREGLLLIRDVLENTSIAILR
                     QICRFHFVNQRLEHKIAAAYVDRLQVRTPSLKQEVGKLSGGNQQKVVLARWLAAKPKV
                     LILDEPTRGIDVGAKAEIYQLIDELANEGLGIMLISSELPEILGLSDRIYVMQNGRIT
                     GELSRAEATEEAVLKLAMIDNLSVA"
BASE COUNT          788 a          923 c          764 g          695 t
ORIGIN      
        1 cttcatgggc gcctgtcagg ctaacagagc tttggctgct gcgaccgccc ggctggcatc
       61 cggtgaatag ccatccgcgc cgatcaaatt ggcataatcc tgagtgatag gagcaccacc
      121 aaccatgatc ttaacctgat cgcgcaggtt cgccgaattc agcgcttcga tggtggcttt
      181 catcccgggc atggttgtcg ttagcaaagc agacatcgca acgatctggg cattatgctc
      241 acgagctgct gtgacgaatt tctccgggga tacatctgca ccgagatcga ttacctcaaa
      301 accggcccct tcaagcatca tggcgaccag gtttttgcca atatcatgca ggtcaccttt
      361 aaccgtgcca atcacgaccc tgcctgcgac cttcacatcc gaatcggcca ggtgcggttt
      421 gagcagcgcc aacccggctt gcatggcccg ggcagcaatc aacatctcgg ggacaaaata
      481 ttcgccctct tcgaagaggc ggcccacttc agccatggct gcaatcatgc cctcattcaa
      541 aataaccgga gccggtatgc cagcctccag agcagcctga actttgacct taacgggctc
      601 gctttgacct tccattacat gggtatagat ttcttgaact aatgcttcca taaatgactc
      661 ctgaaatgat ctactcaccg agaaaataac cagttgccac cagctggtga gcgatgaata
      721 ttttgtttct cagccgatta attttgagta gcaattttgg tgcggacacg ctcagctcgc
      781 gcctggcgcc actgtgaaac agcgactgct gcaaccaaca acaggcccag ggcgacacgt
      841 tggacaaatg tattaaagcc cagcagattc atgccattgt tcagcacacc gaccaggata
      901 accgccagga atgtcccaaa gatcgttcct ttgccacctg aagtagctgc accgcccaga
      961 aataccgctg taattgcctg cagctctaac ccgttcccgt taatcgggtt gccggaggtg
     1021 gtgcgggcag taagcagcac acctgccaga cctgcaattc cacctgaaag catgtacatc
     1081 aagaccttga tcttgttcac attaattccc gccaggcggg ctgctatggt attaccacca
     1141 atcgcataga ctgcccggcc gaaatcggtg tagcgcatga ggatgtgaac caggatggtc
     1201 acagcgacca ggatgacagt cagcaccggg atgcctgtcc atttcgagcc agccaacggt
     1261 gggatgccca ggttggtcaa aaagcgaccc tgcgccagcc aggtaaatgc aggctgggtg
     1321 agaacaccca ccggtttacc atccggagcc aacaagaagg ccaggccagc aaacgcagcc
     1381 agggtgccca gggtagcaat ggttgggttg acccgcagcc tggtaataat aaagccattg
     1441 atcaggccgg cagccatacc tgctaatatc ccagccagaa cagcaataaa gacgctcccc
     1501 ttgggtagaa tacccaatgc gccgatggtc cctaccccgg ccaggacaga tgccgacact
     1561 accgagccaa tggaggcaac ggagccaacc gaaatatcca gcccaccagc gacgatgatc
     1621 accgtctcgc caattgccag caagccaaca atcacgatcg cttgagccag gctgttccac
     1681 aaattctgcc aggagaagaa tttatcgccc ccactcatac caaaccagcc gcttgccacg
     1741 gtgatcaaga aagtcaggac gagcagggca aagatcaagg tcaggttgtc agcgccaata
     1801 acaccgagaa ccttccgaaa accattattt ttactcttcg caggaactgc aatttcgcca
     1861 gttaactctt tgttcataat gtgctcccct gatgggttgt ttccgcgacc tgaggttagg
     1921 ccaccgaaag attatcgatc attgccagtt taagtactgc ctcttcagta gcctcagctc
     1981 tggagagctc gccggtgatc cggccatttt gcatgacata tatccggtcc gaaaggccca
     2041 ggatttcggg cagctcggag ctgattaaca taatccccaa tccctcgttg gcaagctcat
     2101 cgatcagctg gtagatctca gctttagcgc caacatcgat ccctcgcgtg ggttcgtcca
     2161 ggatgaggac tttcggtttg gccgccagcc agcgggccag gacaaccttt tgctggttac
     2221 cgccggataa tttaccgact tcttgtttga gcgaaggcgt ccgcacctgc agcctatcga
     2281 cataagccgc ggcaatttta tgctccaggc gctggtttac aaaatgaaaa cgacatatct
     2341 ggcgcagaat agcgatggaa gtattttcaa gtacgtcccg gatgagcaag aggccttcac
     2401 gcttgcgatc ttcaggcgca aaaccaactc cattggaaat cgcctggtct ggccggcgga
     2461 tattcgtctc gcgaccatcg atttcgatgc tgcccctgag cctgggcatt tcaccaaaga
     2521 tcactttggc cagctcagtt cgacctgccc cgatcaatcc tgcaaagccg ataacttcgc
     2581 cagcattaat gtggaaactc accccaacat gccagttgct atacaggtct tcaactctta
     2641 aaacaaccct gtcggtaccc gatggtgtgc ggtggataaa ttccgaaagc ttgcggccta
     2701 ccatcaagcg gacaatctct ctttcggtaa gctcggcggc aggtttcacc gcaaccaggt
     2761 tgccatcccg caatacagca atccggtcac atagtttcaa gatttctttg atccgatggg
     2821 acacataaat aatcgccacg ccttcatctc ttagccggcg gacaagcgta aataaacgct
     2881 cgacttcatc gtcggtaagg gaggaggtcg gttcatccag agctaacacc cgcaccccag
     2941 atttgagggc gcgcataatt tcaaccagct gcctttgggc tgaagaaagt tcttccccca
     3001 gcagatggat aggcagcgta tcctcaaaac catattctgc caggctttcc tggacgagct
     3061 gctcgagctt gcgtcgatca atcaagccca tttttttagg aagctcacct acccagatat
     3121 tttcggcgac atcgatgccg gggacaatct ctggttcctg gtaaatcacc
//
LOCUS       DATMXK010000086         3113 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_110383, whole genome shotgun sequence.
ACCESSION   DATMXK010000086 DATMXK010000000
VERSION     DATMXK010000086.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3113)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3113)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3113
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110383"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..921
                     /gene="fhcD"
                     /locus_tag="VN363_01610"
     CDS             22..921
                     /gene="fhcD"
                     /locus_tag="VN363_01610"
                     /EC_number="2.3.1.101"
                     /inference="COORDINATES: protein motif:HMM:NF002554.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran--tetrahydromethanopterin
                     N-formyltransferase"
                     /protein_id="HWQ83229.1"
                     /translation="MALLINGVEIEDTFAEAFKMWGTRVIITAKNRKWAWEAATKMTG
                     FATSVIGCKCEAGIEGDVEDTLDGRPGVSVLFFTPSKDDLPKRLMDRIGQTVMTCPTT
                     ACFNGLDAPDKLSIGGQLRYFGDGYQGSKLVNGRRFWRIPVMEGEFLIEAKLGMQRSV
                     GGGNFLVLGEDPDVTLEATERAVEAMKQVPGMILPFPGGIVRSGSKTRSKYKFLIAST
                     NDAFCPTLRGVVDSELPEGVNSVLEIVIDGLNVQAVEEATRVGILAACIPGIRRITAG
                     NYGGTLGQFKIDLHRVLGEGVPS"
     gene            918..1727
                     /locus_tag="VN363_01615"
     CDS             918..1727
                     /locus_tag="VN363_01615"
                     /EC_number="1.2.7.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR03122.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018493 - formylmethanofuran dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formylmethanofuran dehydrogenase subunit C"
                     /protein_id="HWQ83230.1"
                     /translation="MTRLKLNIQSNFPLEVESITPENFSGKSEAEIAALPGLYGNENG
                     VIGDFFTVTGDGSEEIELEGDLANVKYIGAGMKRGKITIHGNVGMHLGARMHGGEIVV
                     HGNAGDWAGAEITGGTIRIHGNAGHGLGGAYRGSRHGMNRGFIWVGGNTGNETGALMR
                     RGLIVVQGRTGDFTGAFMLAGTVICFGGLGSRPGAAMKYGTLVTHQTPELLPTFRYDC
                     VYQPGFLRLILNDLRRKGITVGDELIAGTYRRYSGDFTSLGKGEILVYDQH"
     gene            1714..2679
                     /gene="mch"
                     /locus_tag="VN363_01620"
     CDS             1714..2679
                     /gene="mch"
                     /locus_tag="VN363_01620"
                     /EC_number="3.5.4.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR03120.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HWQ83231.1"
                     /translation="MISINRLTNEIVRKMVQDSDALGLNVLQLANGATVIDAGVKVPG
                     SLEAGRLFAEACLGGLGQVSLTRLEYEEAGKSPFWLPAVSVSVSSPHIACMGSQYAGW
                     AVKREKFFAMGSGPARALYAGEELYTKLDYKDEAEVGILLLEGRKLPKEDVVEYIAQK
                     CRVQPECVTLLIAPTASLVGTVQIAARVVETGMHKMVELGFDVRKVTAGYGVCPLATI
                     AADDLKAIGRTNDGVLYGGQAFYTVQATDEELSELVEKVPSSSSRDYGTPFYDLFQRY
                     GGDFYQIDPLLFSPAEVILNNVTSGRSFAAGKINPALLRTSLLES"
     gene            2683..>3113
                     /locus_tag="VN363_01625"
     CDS             2683..>3113
                     /locus_tag="VN363_01625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83232.1"
                     /translation="MTQPNLQIGILSSQLGWHATALQEALKRHDVIPCYFPIQRLAAW
                     TGEPSQVEAYGQRLEDCQALIVRTIPIGSLEQIIFRMDALHRLEHLGMRILNPPSAIE
                     RTVDKYYTDFLLRDAGILTPRTMVTEDFEQALQAFRVMGDVV"
BASE COUNT          702 a          798 c         1043 g          570 t
ORIGIN      
        1 cagtaggagg tctctaaagt catggcactc ctgataaacg gcgttgaaat tgaagatacc
       61 tttgccgaag ccttcaaaat gtggggcacc cgggtgatca ttacggctaa aaatcgcaag
      121 tgggcctggg aagcagcaac caaaatgacc ggatttgcca cctcggtgat cggctgcaaa
      181 tgcgaggccg ggatcgaagg tgatgtggaa gacacgctgg atggaagacc gggggtgagc
      241 gtattgtttt tcaccccttc caaggatgat ctacccaaac ggttgatgga ccggatcggg
      301 cagacagtga tgacctgccc caccacggcc tgtttcaacg ggctggacgc accggataag
      361 ctctctatcg gcgggcagct gcgctatttt ggggacggat accaggggag caagctggtg
      421 aacgggcgac gcttctggcg catcccggtg atggaaggag aattcttgat cgaggccaag
      481 ctggggatgc agcgctcggt gggcggaggg aacttcctgg tgctggggga agacccggac
      541 gtgaccctgg aggcaaccga gcgggcggta gaagccatga agcaggtgcc gggaatgatc
      601 ctgcccttcc cgggcgggat cgtgcgcagt gggagcaaga cgcgctcgaa atataaattc
      661 ctgatcgctt cgaccaatga tgccttctgc cccacgctgc gaggcgttgt agacagcgag
      721 cttcccgaag gggtgaactc ggtcctggag atcgtaatcg acgggctgaa tgtacaggca
      781 gtggaagaag ccacccgggt aggaatcctg gcggcatgta taccgggcat ccggcggata
      841 accgccggaa attatggcgg cacactgggg caattcaaga tcgatctgca ccgggtgctg
      901 ggagagggtg tgccatcatg accaggctaa agttaaacat ccaatccaac tttccgttgg
      961 aggtcgaatc catcacgccg gagaatttca gcgggaagag cgaagcggag attgcagccc
     1021 tccctggatt atatgggaac gagaacggcg tgatcgggga tttctttacg gtgaccggcg
     1081 acggaagcga ggagatcgag ctggagggcg acctggcgaa tgtcaaatat attggcgccg
     1141 ggatgaaacg gggcaagatc accatccacg ggaacgtagg gatgcacctg ggcgccagga
     1201 tgcacggcgg ggagatcgtg gtgcacggta acgcaggcga ctgggccggg gctgagataa
     1261 ccggcggcac gatccgcatc cacgggaacg cggggcacgg gctgggaggc gcgtatcgcg
     1321 gcagccggca cgggatgaac cgcgggttca tctgggtggg gggaaacacc gggaacgaaa
     1381 ccggggccct gatgcggcgc gggctgattg tagtgcaggg gcggaccggc gattttaccg
     1441 gggcgttcat gctggctggg acggttattt gtttcggcgg cctgggaagc cgcccaggag
     1501 cagccatgaa atacgggacc ctggtaacgc accagacgcc ggagctgctg cccaccttcc
     1561 gctatgactg tgtgtaccag ccaggttttc tgcggctgat cctgaatgat ctgcgccgga
     1621 aagggataac agtcggcgac gagctgatcg ccgggaccta ccggcgctac agcggggatt
     1681 tcaccagcct gggaaaagga gaaattttag tctatgatca gcattaaccg gcttaccaac
     1741 gagatcgtca gaaaaatggt ccaggacagc gatgccctgg gtttaaatgt gctgcagctc
     1801 gccaatggag cgaccgtaat cgacgccggc gtgaaagtgc cgggcagcct ggaagcaggc
     1861 aggcttttcg ctgaggcgtg cctgggaggg ctgggacagg tgagcctgac ccggctggag
     1921 tatgaggagg cgggaaaaag ccccttctgg ctgccagcag tgagcgtcag cgtgagcagc
     1981 ccccacatcg cctgcatggg atcgcagtac gcaggctggg cggtcaaacg cgagaaattc
     2041 ttcgccatgg gctcaggccc ggcacgggcc ctgtacgccg gggaagagct gtacaccaaa
     2101 ctggattaca aagatgaggc agaagtcggc atcctcctgt tagaggggcg caagctgccg
     2161 aaagaagatg tggtcgaata tatcgcccaa aaatgtcgcg tccaaccgga atgtgtgacc
     2221 ctgctgatcg cgccgacagc atccctggtg ggtacggtgc agatcgcggc gcgggtggtg
     2281 gaaaccggga tgcacaaaat ggtagagctg ggcttcgatg tgcgcaaggt gaccgcggga
     2341 tatggagttt gcccactggc gacgatcgcg gcagatgacc tgaaagcgat cgggcggaca
     2401 aatgacggcg tgctctatgg aggccaggca ttctataccg tgcaggcgac agacgaagag
     2461 ctgagtgagc tggtggagaa ggtgccttca tcaagctcgc gagattacgg gacccccttc
     2521 tacgatctct tccagcgcta tggcggggat ttctaccaga tcgacccgct gctgttcagc
     2581 ccggcggagg tgatcctgaa taatgttacc agcgggcgaa gttttgcagc cggcaagatc
     2641 aacccggcac tgctgcgcac atcgttgctc gagagctaat ccttgactca gccgaacctg
     2701 caaatcggga ttttatcatc ccaattaggc tggcatgcga ctgcactgca agaggcgctt
     2761 aagcgccacg atgtaatccc gtgctacttc ccaattcaaa gactggcggc ctggacaggg
     2821 gagccgtctc aagttgaagc atatggtcag cgcctggagg actgccaggc gctgatcgta
     2881 cgcaccatcc cgattgggtc gctggaacag atcatctttc gcatggatgc gctgcaccgg
     2941 ctggaacatc tgggaatgcg aatcctgaat ccacccagcg cgatcgagcg cacggttgac
     3001 aagtattata cggatttctt gctgcgagat gcgggaatcc tgactccgcg gaccatggtg
     3061 acggaggact tcgaacaggc actgcaggct ttccgggtga tgggggatgt agt
//
LOCUS       DATMXK010000087         2000 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_98549, whole genome shotgun sequence.
ACCESSION   DATMXK010000087 DATMXK010000000
VERSION     DATMXK010000087.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2000)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2000)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2000
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98549"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VN363_01630"
     CDS             <1..363
                     /locus_tag="VN363_01630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559246.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83233.1"
                     /translation="ILLNVNIPALPLEQIHGLRTTRQGLRVYRDRLDQRIDPRGQPYF
                     WIGGDAPTGVVESGTDFGALHEGFVSITPLQLDLTAYPAQHMLKNWQWQAGQPTENQQ
                     MAVPFSASELALDQSRSK"
     gene            366..662
                     /locus_tag="VN363_01635"
     CDS             366..662
                     /locus_tag="VN363_01635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83234.1"
                     /translation="MSLPVYPASDLRWNILQEGAVRNGSSAAYLLLLSAVDQGWSIRQ
                     PVRVFPNPVHPEIPSCHISLERKPGRQTRELAIPFSAELETYLTAEQIAVRIAR"
     gene            710..1858
                     /gene="chrA"
                     /locus_tag="VN363_01640"
     CDS             710..1858
                     /gene="chrA"
                     /locus_tag="VN363_01640"
                     /inference="COORDINATES: protein motif:HMM:TIGR00937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015109 - chromate transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015703 - chromate transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chromate efflux transporter"
                     /protein_id="HWQ83235.1"
                     /translation="MFLRLGLTAFGGPAAHIAMYHDEVVVRRKWLDEAQFLDLLAATN
                     LIPGPNSTEMTIHIGYQRAGWQGLLAAGSLFILPAVLITTAFAWVYARYGATPQAAWL
                     LFGVKPVVIAIILQALWGLGKKAIVGTFSAAIALAATAAYFLNVDTLLTLFLGGLLMM
                     LYQNRQRLKTAIGESLPALLLPVLTQAQWNLGAILAAPGLQTAQPFSLGRLFLAFLKI
                     GSILYGSGYVLVAFLQDEFVTHLGWLNPQQIIDAIAIGQVTPGPVFSTATFIGYQLGG
                     LPGAALATLGIFLPSFIFVALSNPLIPRLRSSPWAAGFLDGVILASLGLMAAVTFELA
                     QDAFSGPFAIAIALLSALLLFRFKVNTTWIILGGAFLGVLAHFLGWVA"
BASE COUNT          357 a          656 c          538 g          449 t
ORIGIN      
        1 atcttactga acgtcaatat tcccgccctc cccctggaac agatccacgg cctgcgtaca
       61 actcgccagg gcctgcgcgt ttaccgcgac cgcctggacc agcgcattga cccgcgcggc
      121 cagccatatt tctggatcgg cggcgatgcc ccaaccggag tggttgaatc ggggaccgat
      181 tttggcgcgc tgcatgaagg attcgtctcg atcaccccgc tgcagctcga tctgactgcc
      241 taccctgccc agcatatgct gaaaaactgg cagtggcagg ctggccagcc caccgaaaac
      301 cagcaaatgg cggttccttt ttccgccagc gagctcgccc tggatcaatc gaggagcaaa
      361 tgagcatgag cctgcctgta taccctgcca gcgatctgcg ctggaatatc ctgcaagaag
      421 gggcggtccg caatggatca tccgctgcct acctgctcct gctttcggca gtcgatcagg
      481 gctggtccat tcgccagccc gtccgggtat ttccaaatcc ggtccacccg gagattccca
      541 gctgtcacat ttccctggaa cgcaaaccag gccgccagac ccgcgagctc gccattccct
      601 tttcggcaga gctggagact tacctgaccg cagagcaaat cgcggttaga attgcgcgat
      661 aaacgccaat ccattcactc cccaccccca ccttggtgaa gtcgccagat tgttcctgcg
      721 ccttggtctg accgcgtttg gcgggccggc agcgcacatt gccatgtatc atgatgaggt
      781 cgttgtccgc cggaaatggc tggatgaggc gcagttcctg gatttgttgg cggctaccaa
      841 tctgatcccc ggtcccaatt cgaccgaaat gaccatccac atcggctacc agcgcgccgg
      901 atggcagggc ctgctggccg caggcagcct gtttatcctc cccgccgtgc tgatcaccac
      961 cgccttcgcc tgggtttacg cccggtacgg cgccacacct caggcagcct ggctgttatt
     1021 cggtgtcaaa ccggtcgtca tcgccatcat cctccaggcc ctgtggggcc tggggaagaa
     1081 ggccatcgtg ggcactttca gcgctgccat cgccctggca gccaccgcag cctattttct
     1141 gaatgtggat accttgctga cgcttttcct gggtgggctg ctgatgatgc tctaccagaa
     1201 ccgccagcgc ctgaaaacag ccatcggtga aagcctgcct gcgctgctcc tccctgtcct
     1261 tacgcaggcg cagtggaatc tgggagcaat tctggctgcg ccaggattgc agaccgccca
     1321 gccgttcagc ctgggccgtt tattccttgc tttcttgaaa attggctcga tcttatacgg
     1381 cagcgggtat gtcctggttg cttttctgca agatgaattt gtcacccacc tgggctggct
     1441 gaacccccag cagatcatcg acgccattgc catcggtcag gtcacccccg gcccggtttt
     1501 ctcaaccgcc accttcatcg gttaccagct gggtggcctg cccggggcag ccctggccac
     1561 cctgggtatt tttctgccgt cgtttatttt cgtcgccctc agcaatccgc tgatcccccg
     1621 cctgcgcagc tcgccctggg cggcgggatt tctggatgga gtcatcctgg cttcgctggg
     1681 tttgatggct gcagtcacct tcgagctggc gcaggatgct ttcagcggac cctttgccat
     1741 cgctatcgca ctgctctccg ccctgctctt attccgcttt aaggtgaaca ccacctggat
     1801 catcctgggc ggggcattcc tgggcgtgct agctcacttt ctgggttggg ttgcttaacc
     1861 tctcgcctgg cgcagcaggc taaagctgaa tccctcgaat tattccagca gcaggcgagc
     1921 caaagatgcc tccagccagg caataaactg ctctcgcgaa taatttcgat cgcgggtcag
     1981 taacaggtac acctcaggac
//
LOCUS       DATMXK010000088         2812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_133278, whole genome shotgun sequence.
ACCESSION   DATMXK010000088 DATMXK010000000
VERSION     DATMXK010000088.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2812
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133278"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2812
                     /locus_tag="VN363_01645"
     CDS             <1..>2812
                     /locus_tag="VN363_01645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83236.1"
                     /translation="RRVATLAAAFYAAMALPIQQAHFFTMDTFINLFTLLSIYFAVRI
                     STDQRAWATAVVDPADTTSLAGLRGTWQTLKQHPLFLPSLGFGLAYGMAVASKLNAAP
                     VALALPAAVMIRIAGLPRLRRNQAIIRAIPFVVLAAALSILIFRLCQPYAFSGPGFLG
                     LSPNPQWIAQLKELRTQSSGDVDFPPAMQWARRDITFSGKNMVLWGMGLPLGILVGFG
                     FLWAGLRMLTHRKDRAWRQHALLWGWTGLYFTWQSLALNPTMRYQLPIYPTLAIFAGW
                     AVIALWDAAQNVSLRRWLTPGRLRGSALIIGTFVLLATYGYAYGFSTIYTRPITRVEA
                     SRWIYDHVAGPINLKIETQKGEEVQLLPVSYDYPITPGYPLTTTFQAQTSGVLREIYI
                     PHLRDTQDGDYQRTIHLTISNQGEPGSALASAALAADLSPQTDARGDAYTLQLDNPLT
                     LVEGQLYSLELSVDGTPDQVAVDGDFKLAFETADPAGDNLVLTPGGFFLLSSQPFATE
                     LAPARDVHLTELTIHQVSVLSSGTEPLTLAVSLTTPEGLIPGEEPVDTSQRDTPLATV
                     VGSVTLDTSSTQPLDYQIPMDIPLPLTAGQSYRLQVFLLSQQPNLELSGDSLANEGEW
                     DDGLPLRMDSYDPFGGIYPQDLNFNMYWDDNADKQARFIRILNESDYIVISSNRQWGS
                     LTRIPERFPLTTTYYRELLGCPEDQTIYWCYAVAEPGRFNGTLGFELVQIFQSNPKLG
                     FIELNDQFAEEAFTVYDHPKVLIFKKTADFNSERMAAVLNAVDLSRVLRIAPMRFPDR
                     PADLMLSPERVAEQRAAGTWTELFNPQSWINRSQLLAVVMWYLCFSLLGWALYPTLRH
                     LLPGLPDRGYALARTAGLLIFAYLAWLAGSTGVGYTRPVIAAIFGLLLASGLIFGFKD
                     RQDLISEFKRHWRYVLFVELLAL"
BASE COUNT          559 a          836 c          766 g          651 t
ORIGIN      
        1 acaggcgtgt cgcgactctg gctgcagcct tctatgccgc gatggcgctg ccgattcaac
       61 aggcgcattt tttcacgatg gacacgttca tcaacctgtt cacgctgctg tctatttatt
      121 ttgcagttcg catcagcaca gaccagcgcg cgtgggcgac agcggtagta gaccctgcag
      181 ataccacctc tctggccggt ctgaggggaa cgtggcaaac tctaaaacag catccattat
      241 ttctccccag cctggggttc ggtctggcgt atgggatggc agttgcctcc aaacttaacg
      301 cagctcctgt cgccctggca cttccggccg cggttatgat ccggattgcc ggtctgccgc
      361 gcctcaggcg taatcaagca attattcggg cgatcccctt tgtggtgctg gcggctgcac
      421 tgagtatcct gatcttcagg ctttgccagc cttacgcctt cagcggtccg ggcttcctcg
      481 gattatcacc caacccccag tggatcgccc agcttaaaga attacgcacc cagagcagcg
      541 gggatgtgga tttcccgcct gccatgcagt gggcgcgccg ggatatcaca ttttccggaa
      601 aaaacatggt cttatggggg atgggccttc ccctcggcat tctcgtaggg ttcggcttcc
      661 tttgggcagg cttgcggatg cttacccacc gtaaagatcg cgcctggcgc cagcatgccc
      721 tgttgtgggg gtggaccggg ctctacttca cgtggcaatc tctggcgctc aaccccacca
      781 tgcgctacca gctgccgatc tatcctaccc tggctatttt tgccggctgg gcagtgattg
      841 cgctgtggga tgccgcccaa aatgtctcct tacgccgctg gctgacgccc ggacggctgc
      901 gtggatcggc tcttatcata ggaacctttg ttcttctggc tacgtatggc tacgcttacg
      961 gattcagcac catctatacc cggccaatta cccgggtgga ggcatctcgc tggatctatg
     1021 atcatgtcgc cggtccgatt aatttaaaga tcgaaaccca aaaaggtgaa gaagtccagc
     1081 tcctgccggt cagctatgat tatccgataa cgcctggtta tccactgacg accacttttc
     1141 aagcccagac cagcggggtg ctgcgagaaa tctatatccc ccacctgcgc gatactcagg
     1201 acggagatta ccaacgcacg atccatttga cgatctcgaa tcagggcgag cctggatcgg
     1261 ccctggcgag cgcagcgctg gcagctgatc tatcccccca gaccgatgcg cggggagatg
     1321 cttacacgct tcaattggat aatccgctta ctctggttga agggcagcta tattccctgg
     1381 aactgtccgt ggatggaacc cctgaccagg tagccgtaga tggcgatttc aaactggcat
     1441 tcgaaacggc agacccggcc ggcgataacc tcgtcctgac cccgggggga tttttcctgc
     1501 tctccagcca gccttttgca accgaattgg cgccagccag ggatgttcac ctcaccgaac
     1561 tcaccatcca ccaggtcagc gtactttcga gcgggaccga gccacttact ctggcagtca
     1621 gcctgaccac tccagaaggt ctgatcccgg gtgaagagcc ggtcgacacc agccagaggg
     1681 atactcccct ggcgaccgtg gtagggtctg taaccctgga tacgtccagc acacagccat
     1741 tggattacca gatcccaatg gatatcccct tacccctgac tgccgggcaa tcctaccggc
     1801 tgcaggtctt cttgctttca cagcagccca atctggagtt aagcggagac tcgttggcga
     1861 acgagggtga atgggacgat ggactgccgc tgcggatgga cagttatgat cctttcggcg
     1921 gcatctaccc ccaggatttg aatttcaaca tgtattggga tgacaatgcc gataaacagg
     1981 cgcggtttat ccgcatcttg aatgaatcgg actacattgt gatcagctcc aaccgccagt
     2041 ggggcagcct gacgcggata cccgagcggt ttccactgac aaccacctat tatcgtgagc
     2101 ttctgggctg tccggaggac cagaccattt attggtgcta tgctgtggca gaaccaggca
     2161 ggttcaatgg cactttgggg ttcgagctgg tgcaaatatt ccaatccaat cctaaactcg
     2221 gatttatcga gcttaacgac cagttcgccg aagaagcctt caccgtctac gaccatccca
     2281 aggtgctgat cttcaaaaag acagccgatt tcaattctga acggatggct gcagtcttga
     2341 atgcggtcga tctcagccga gtcctgcgga ttgcgcctat gcgttttcca gaccgcccgg
     2401 cggacctgat gttatccccg gaacgggtgg cagaacagcg cgcagcagga acgtggaccg
     2461 agcttttcaa ccctcagagc tggataaacc ggtcccagct cctggctgtc gtgatgtggt
     2521 acctgtgctt cagcctgctg ggatgggcgc tctatcccac cttgcgccac ctgctccccg
     2581 gtctgcccga ccgcggttat gccctggcac gcactgcagg tttgctgatt tttgcctatc
     2641 tggcctggct ggcaggctcg accggcgttg gctatacgcg cccggtgatc gcagccatct
     2701 ttgggctgct gctggcttcg ggtctgatct ttggattcaa agaccggcaa gacctgataa
     2761 gcgaattcaa acgtcactgg cggtatgtgc tgttcgtcga gctcctggcg ct
//
LOCUS       DATMXK010000089         4457 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_77122, whole genome shotgun sequence.
ACCESSION   DATMXK010000089 DATMXK010000000
VERSION     DATMXK010000089.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4457)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4457)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4457
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77122"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(156..1418)
                     /locus_tag="VN363_01650"
     CDS             complement(156..1418)
                     /locus_tag="VN363_01650"
                     /inference="COORDINATES: protein motif:HMM:NF024873.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease H-like domain-containing protein"
                     /protein_id="HWQ83237.1"
                     /translation="MPSLADRLKSLGVQVGAQNLPPRQAEYPFPIEKVVGGELLYTHF
                     GPTYQVEERFPAGHLHGSLGLLTTSPMDALAAWTGDERVRNLDPRRIAFIDTETTGLS
                     GGTGTYAFMIGIGRFIGDEFQTVQLFMRDPAEEPADLAALEEYLAPAQALVTFNGKTF
                     DLPLLRTRFTTHGLKPSFSDLAHIDLLHISRRLWRDRLPSRTLGNLEYQILGAQRTED
                     DVPGWMIPSLYFDYLRSGDARPLRGVFYHNKIDVVSMAALLNCIASLLADPSGASLPD
                     QSDLLAIAKFYDALGDAQAAAGLYHECLQRSLSETQQVEAMLRLAQIRKRAGEDAAAI
                     ALWKQAAFHWNLDAHIELAKAYEHRLREPRSALEWTQAAIQLVNHSDSTPFDKRTRLA
                     ELEYRQARLERKLAGKATLADETLNPAE"
     gene            complement(1437..3995)
                     /locus_tag="VN363_01655"
     CDS             complement(1437..3995)
                     /locus_tag="VN363_01655"
                     /inference="COORDINATES: protein motif:HMM:NF012492.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DEAD/DEAH box helicase"
                     /protein_id="HWQ83238.1"
                     /translation="MSVASQISAWRADPSIGDNISFWQQIPARPASLTSIPDDLPEPL
                     KRSLQRQGIASLYSHQLKAYRLARSGSNVALVTGTASGKSLAYNLPVLAGLLNDPRGR
                     ALYLFPTKALAQDQLTSLKTLLNLAGDLSVPLPAPAIYDGDTPTAQRALIRQNAQLLL
                     SNPDMLHMGILPHHTSWAGFFENLRYVVLDEIHTYRGVFGSHIANVLRRLMRVAAHYG
                     SRPQFLLTSATIANPQEHASRLIEAPVSVVAEDGSARGPKHFLLYNPPIIDPRLGLRR
                     SALQEAVRLSDDLLTAGVQTIVFARSRRTVEIVLTYLREKATPAGGWENEQETLELRG
                     YRSGYLPGMRRQIEAGLRRGDVRAVVATNALELGIDIGQLGAAVLVGYPGSIASTWQQ
                     AGRAGRGSELSAAVLVATPAPLDQYLATHPEYLFERSPEQALIDPDNPLILLSHLRCA
                     AFELPFRQGEKFGSLAAGEVAEYLEFLASEGILHASNDRFYWMADQYPAQQISLRNAS
                     PSQVLLQAAEPGDPENLRTIGQIDQGSALWMVHPNAVYLHESQTYFVEELDLEHGQAR
                     LAPFGGDYYTLSMQDTQVQKLSLTAEAAIPGGLKASGELQVTTRVTGFRKIRWHTHEV
                     LGVEPLDLPPSELQTTGYWITLSDECVAGLREAGLWTNDSNDYGPDWDAIRQLVRQRD
                     DYRCQGCGMQETNRAHDVHHLVPFRQFASVFEANQLNNLVSLCPSCHKRAEAAVRMRS
                     GLAGLSYVMSNLAPIFLMCDTSDLGAHSDPQSPISDNKPVVVVYDMVPAGIGLSQRLY
                     ESHSALIAAASELIQSCACEDGCPSCVGPGGEQGSGSKQATLAILESLQASTNP"
     gene            complement(3982..>4457)
                     /locus_tag="VN363_01660"
     CDS             complement(3982..>4457)
                     /locus_tag="VN363_01660"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWQ83239.1"
                     /translation="LDPHGPVCLCGKRGCVERLASGPYLAQDARQALSQEPDQGPELL
                     RLCGGFYDQITAEMLSRAARQGDPLASRLLARSAWAIGTAIGNAANLLNPQRAILGGG
                     VTKAGRAWWQGVRSAARQTMLPEVSIEIVPAKLGDDAPLWGAAVLALECVANERR"
BASE COUNT          810 a         1265 c         1569 g          813 t
ORIGIN      
        1 ccgatcagca gcgcggaatc ggtcatctgg cggacgaata acggcaggat ggtgacgaag
       61 gaggtgaatc ccatcgccag accaaaaaaa gccccgtcga gcaggttaac tttgatattg
      121 tatttctgga ggggtgtgat gggggtattg agcatttatt cggccgggtt cagggtttcg
      181 tcggcgaggg tggctttacc ggctaatttg cgttccaggc gcgcctggcg gtattcgagc
      241 tccgccagcc gggtgcgctt atcaaaaggc gtggaatcgc tgtgattgac cagctggatg
      301 gctgcctggg tccactccag ggcggagcgc ggctcgcgca ggcggtgctc gtaggctttg
      361 gcaagctcga tatgggcgtc caggttccag tggaaggcag cctgcttcca taaagcaatc
      421 gccgcggcat cctcgccggc ccgcttgcgg atctgagcca gccggagcat ggcttctacc
      481 tgctgggttt cagagagact gcgctgcagg cactcatggt acaggccggc ggcggcctgc
      541 gcatctccga gggcgtcata gaatttggcg atcgccagca ggtcgctctg gtcgggcagg
      601 gacgcaccgg atggatcggc gagcaggctg gcaatgcagt tcaacagggc tgccatcgag
      661 acgacatcga ttttattgtg gtaaaagacg ccccgcagcg ggcgagcatc gccgctgcgc
      721 aggtaatcga aatacaggga ggggatcatc cagccaggga cgtcgtcctc ggtgcgctgg
      781 gcgcccagga tctggtattc aaggttgccc agggtgcggc tgggaagtcg gtcgcgccac
      841 agccggcggg agatatgcag caggtcgatg tgagccaggt cagagaagct gggtttcaag
      901 ccgtgggtcg tgaagcgggt gcgcagcagg ggcaggtcga aggtctttcc gttgaacgtc
      961 accagggcct gggcgggagc cagatattct tccagggctg ccaggtcggc gggctcttcg
     1021 gcggggtcgc gcatgaaaag ctggacggtc tgaaattcat cgccaatgaa ccggccgatg
     1081 ccgatcatga aggcataagt tccggttccg ccggaaagcc cggtcgtctc ggtatcgatg
     1141 aaagcaatcc tgcgggggtc cagattgcgc actcgctcat caccggtcca ggctgcgagg
     1201 gcatccatcg gggaggttgt aagcaggccc agactgccgt ggaggtgacc ggcggggaaa
     1261 cgctcttcaa cctggtaggt ggggccgaaa tgggtgtaaa ggagctcgcc accaacgact
     1321 ttctcgatgg ggaaggggta ctccgcctgg cggggaggta aattttgggc gccaacctgc
     1381 acgccgaggg atttcaaacg atccgccagg gatggcattc tgactcctga tccggattaa
     1441 gggttggtgg aagcctgcag gctttccaga attgccaggg tggcctgctt gctgccgctg
     1501 ccctgctcac cgccggggcc cacacaggat gggcagccgt cttcacaggc acaggattgg
     1561 atgagctcgc tggctgcggc gatcagtgcg ctgtgcgatt catacaggcg ctggctcagg
     1621 ccgatcccgg ccggaaccat atcatacacc acaacgaccg gtttattatc gctgattggc
     1681 gactgcgggt cgctgtgcgc acccaggtcg gaagtatcgc acattaagaa gattggcgcc
     1741 agattgctca tcacatagga taggccggct aacccgctgc gcatacgcac ggcggcttcg
     1801 gcgcgtttgt ggcagctcgg gcacaggctg accaggttgt tgagctggtt ggcctcgaaa
     1861 acgctggcaa actggcggaa aggcaccaga tggtggacgt catgggcgcg attcgtctct
     1921 tgcatcccgc agccctggca gcgatagtca tcccgctggc ggacgagctg gcggatggcg
     1981 tcccaatccg gcccataatc gttgctgtca ttggtccaca gcccggcttc gcgcaggcct
     2041 gccacacatt cgtcagacag ggtgatccag tagccggtgg tttgcagctc actgggcggc
     2101 aggtcgagcg gctcgacccc caggacttcg tgggtatgcc agcggatctt gcgaaagccg
     2161 gtgacccggg tggtgacctg cagctcaccg ctggctttca acccgccggg gatggctgct
     2221 tcggcagtca ggctgagttt ttggacctga gtatcctgca tggacagggt gtaataatcg
     2281 ccgccgaacg gcgccaggcg cgcctggccg tgctccaggt cgagctcttc gacaaaataa
     2341 gtctggcttt cgtgtagata aacagcgttc ggatggacca tccacagcgc gctgccctgg
     2401 tcgatctgac cgatggtgcg caaattttca ggatcgccgg gctcggcagc ctgcagcagg
     2461 acctggctgg gtgaggcgtt gcggagcgag atctgctggg cggggtactg gtctgccatc
     2521 cagtaaaagc ggtcgtttga ggcgtgcaga atgccctcac tggccagaaa ttccaggtat
     2581 tcagccactt cgccggcagc caggctgcca aacttttcac cctgccggaa gggcagctcg
     2641 aaggcagcgc agcgcaggtg actgagcagg atcagcggat tatccgggtc gatcagagcc
     2701 tgctcgggtg agcgctcaaa gagatattcg gggtgggttg ccaggtactg gtccaggggg
     2761 gcaggtgtgg cgaccaggac tgccgcggaa agctcggagc cgcgcccggc gcgcccggcc
     2821 tgctgccagg tagaggcaat cgagccgggg tagcccacca gcaccgcggc acccagctgg
     2881 ccgatgtcaa tccccagctc gagggcattg gtggcgacca cggcgcgcac atccccgcgg
     2941 cgcaagccgg cttcgatctg gcggcgcatg ccgggcaggt agccgctgcg gtagccgcgc
     3001 agctcgaggg tctcctgctc attttcccag ccgccggcag gggtggcctt ttcgcgcagg
     3061 taggtcagga cgatctctac ggtccggcgc gagcgggcga agacgatcgt ctggacgcca
     3121 gcagtaagca gatcgtcgct caggcgcaca gcttcctgca gggcgctgcg gcggaggccc
     3181 aggcgcggat cgatgatggg tggattgtag agcagaaaat gcttcgggcc gcgggctgag
     3241 ccatcttctg ccacaacgct gaccggggct tcgatcaggc gcgaggcgtg ctcctgggga
     3301 ttggcgatgg tcgccgaagt aagcaaaaac tggggccggc tgccataatg ggcggcaacc
     3361 cgcatcaggc ggcgcaggac attggcaatg tgtgagccga agacaccccg gtaggtatgg
     3421 atttcgtcga ggaccacgta gcgcaggttc tcaaaaaagc cggcccagct ggtgtggtgg
     3481 ggcaggatgc ccatgtggag catgtccggg ttggaaagta gcagctgagc gttctggcgg
     3541 atgagggcgc gctgggcggt gggggtatcg ccatcgtaaa tcgccggggc gggcagcgga
     3601 acgctcaggt caccggccag gttgagcagt gtcttcaggc tggtgagctg gtcctgggcc
     3661 agggctttgg ttgggaagag atacagggcg cgtccccggg gatcgttcaa caggccggcc
     3721 agaacgggca aattgtaggc cagactttta ccgctggctg ttccggtgac cagggcgacg
     3781 ttgctgccgg agcgagccag ccggtaagct ttgagctggt ggctgtaaag tgaagcgatc
     3841 ccctggcgct gcaggctgcg cttcagcggc tcgggcaggt cgtccgggat tgaagtcagt
     3901 gaagcaggcc gggccgggat ttgctgccag aagctgatat tatcgccaat gctggggtcg
     3961 gcgcgccagg cactgatctg gctagcgacg ctcattggct acacactcca gcgccagcac
     4021 agccgcgccc cacagggggg cgtcatcgcc cagcttggcg ggcacaattt cgatggatac
     4081 ttcgggaagc atggtctggc gggcggcaga ccgcacaccc tgccaccatg cgcggccggc
     4141 tttggtcacg ccgccgccca ggatggcgcg ctgcgggttg agcaggttgg cggcattgcc
     4201 gatggccgtg ccaatcgccc aggcgctgcg cgccagcagg cggcttgcca gcgggtctcc
     4261 ctgccgggcg gcccggctga gcatctcggc tgtgatctgg tcgtagaagc cgccgcacag
     4321 ccggagcagc tcgggtccct ggtctggttc ctggctcaaa gcctggcggg catcctgggc
     4381 gaggtaaggt ccggaagcca ggcgctcgac gcagccgcgt ttcccgcaca ggcacaccgg
     4441 accgtggggg tcaaggg
//
LOCUS       DATMXK010000090         2027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_112294, whole genome shotgun sequence.
ACCESSION   DATMXK010000090 DATMXK010000000
VERSION     DATMXK010000090.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112294"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1282)
                     /locus_tag="VN363_01665"
     CDS             complement(<1..1282)
                     /locus_tag="VN363_01665"
                     /inference="COORDINATES: protein motif:HMM:NF012422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LuxR C-terminal-related transcriptional
                     regulator"
                     /protein_id="HWQ83240.1"
                     /translation="MSDHIELSEREREILRLVATGASNKEIASRLFISANTVKVHLRN
                     IFTKIEATSRTEAAMYAANIGLVDGIAPVKEEEVIDDPAPESIPVLEALPVPEIVPGR
                     RLSLLWVGLVGLVILVLVVSVPNLLNRFPPPGSTPFAENPAVNQEPADWQTAAPLPAA
                     RAGLALAVAEDRIYAIGGEAVHGVIGDVTYYDSTADTWENASLKPTPVTDIQAAVLGG
                     RIYVPGGKIPNGSISAGLEIYDPRADSWSAGADLPAPRSAYGLVAFEGSIYLFGGWDG
                     EKYVNTVYAYDPEQDLWEPRQSLPAPVGYPGTVVAGGRIYLLGGFDGQRAVVDTYQYL
                     PEAAASGEAAWRKLKPLPQGRYAMGVTSIADIIYVVGGISNQSDLPFLEYILPRDEWI
                     PFSPGEKFDWSHGGMTILGTDLHILGGLGGEEYLT"
     gene            1450..1521
                     /locus_tag="VN363_01670"
     tRNA            1450..1521
                     /locus_tag="VN363_01670"
                     /product="tRNA-Gly"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1482..1484,aa:Gly,seq:ccc)
     gene            1657..2025
                     /locus_tag="VN363_01675"
     CDS             1657..2025
                     /locus_tag="VN363_01675"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559607.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ83241.1"
                     /translation="MTISRTVLYVEDNLDNRTLIRRILQVEGYTVLEARDAAQALEII
                     DSTTPDLILMDINMPDMDGYTLTSHIKAMSGKQNIPIIAVTANVMRGDREKTLEAGCD
                     GYIQKPIDVDVLAAQISRYL"
BASE COUNT          512 a          556 c          514 g          445 t
ORIGIN      
        1 gggtaaggta ttcttcaccc cccaacccac ccagaatatg caggtcggtg cccaggatgg
       61 tcattccccc atgcgaccaa tcgaactttt caccgggtga aaatgggatc cactcatccc
      121 tggggaggat atattccagg aacggcagat cagactggtt gctgatccca ccaacgacat
      181 agatgatgtc ggcgatactg gtcactccca tggcatagcg accttgcgga agcggtttta
      241 attttcgcca ggcagcctcg ccggatgctg ctgcctcggg tagatactgg taagtatcga
      301 ctacagcccg ctgtccatca aaaccaccca ggagatagat ccggccaccg gcaaccaccg
      361 tacctggata acctaccgga gcaggcaatg actgccgggg ttcccaaaga tcttgctcgg
      421 gatcatacgc ataaacagtg ttgacatact tttcgccatc ccacccgcca aataaataaa
      481 tactcccctc aaatgcaacc aatccataag cgctgcgcgg cgccggcagg tctgctcctg
      541 cggaccagga gtctgcccgg ggatcgtaga tttccagacc agcgctgatg gatccattgg
      601 gtatcttccc cccaggaaca tagatacggc cgcccaggac ggctgcctga atatctgtga
      661 ccggggttgg ttttaggctg gcattctccc aggtatctgc ggttgaatca tagtaggtca
      721 catcgccgat cacaccatgc acagcctccc caccaatcgc atagatccga tcctccgcca
      781 ccgccagcgc cagacctgcg cgcgcagccg ggagcggggc tgctgtttgc caatcggcag
      841 gttcttgatt gactgccggg ttctcagcga agggagtgct gcctggcgga ggaaaccgat
      901 taagcaggtt tggaacactg accaccaaca cgaggataac cagacctacc agcccgaccc
      961 acaacaggga cagcctacgg ccagggacga tctctgggac cggtaaggct tcaagcacag
     1021 ggatggattc tggagccgga tcatcgatta cctcttcttc cttgaccggg gcaatcccat
     1081 ctaccagtcc aatattcgcg gcatacattg ccgcttcagt gcgtgaagta gcctcgattt
     1141 ttgtgaagat attgcgcagg tggactttaa ccgtattggc gctgatgaat aacctggacg
     1201 cgatctcctt attactggcg cctgtagcaa ccagccggag gatctcgcgt tcgcgttcac
     1261 tgagttctat atgatcagac ataggaggga atgcatctct ggagtgtcgg ggtcaacgcc
     1321 attttgagcc tgggaatgat tataacattc tccgatatgg atcaactttt aaccgcccag
     1381 caggcctctc attagggcag gctgaaactt gacgcagcgg atcagccgcg gtaaaattca
     1441 cgcactcatg cgggcatagt tcagtggtag aacgtcacct tcccaaggtg aatgtcgtgg
     1501 gttcgagtcc cactgcccgc tcagaaaaac ctgctaaaat tgcaggtttt ttgattctta
     1561 caaaactgta aaacaaggtt agcagagaaa gcatctaaac taatgtatag tatagactga
     1621 atgccaggaa agaatgatct atccggagca gctctgatga ccatttccag aacggtcctg
     1681 tatgttgaag ataatctcga taatcgaaca ttgatccggc ggatcctgca ggttgaaggt
     1741 tacacagtac tggaagcccg cgatgccgct caagcgttgg agattatcga ttcaacaacc
     1801 cctgatctaa tcttgatgga tatcaatatg cccgatatgg atggttatac actgacctcc
     1861 cacataaaag cgatgtcagg aaagcagaac atccccatta tcgccgtgac agcgaatgta
     1921 atgcgcggcg atcgggagaa aaccctcgaa gccggttgtg acggctacat ccaaaagccg
     1981 attgatgtag atgtcctggc ggcacaaatc agccgctacc tgtaact
//
LOCUS       DATMXK010000091         4405 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_145018, whole genome shotgun sequence.
ACCESSION   DATMXK010000091 DATMXK010000000
VERSION     DATMXK010000091.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4405)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4405)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4405
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145018"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..621)
                     /gene="npdG"
                     /locus_tag="VN363_01680"
     CDS             complement(<1..621)
                     /gene="npdG"
                     /locus_tag="VN363_01680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADPH-dependent F420 reductase"
                     /protein_id="HWQ83242.1"
                     /translation="MSPSNPLPTLAILGGTGKEGPGLALRWASAGYPILIGSRQAEKA
                     EATAAELNAKLGRDCARGLENSEAARQADICVLTVVATAHQAALESLKDDLQGKILVD
                     TTARVDFRDPHPPAAPSAGRIAQDLLGAGVRVAAAFQNVPASALKKNLGEKLDVDVLV
                     CADDLETARVVMQLAEAAGMNAYYAGSLENALIVEGLTSLLISINKH"
     gene            complement(636..1994)
                     /gene="ssnA"
                     /locus_tag="VN363_01685"
     CDS             complement(636..1994)
                     /gene="ssnA"
                     /locus_tag="VN363_01685"
                     /inference="COORDINATES: protein motif:HMM:NF005540.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="putative aminohydrolase SsnA"
                     /protein_id="HWQ83243.1"
                     /translation="MSEYLITNGILITWERENRVLESQAVYLKEDRIAEIGPQADLLA
                     RYPDAEVLDAHGQYVMPGNICAHTHFYGAFSRGMGIPGAAPKDFPDILRKLWWPLDKA
                     LDMDAVRASAEVMLVDAIRHGTTTLIDHHASPNAIAGSLDTIAEAVDLSGLRAVLCYE
                     VTDRDGAEKSQAGIAENVRFINHLKEAPHPRLGATFGLHASLTLSDETLAKCVRAAPK
                     GTGFHIHVAEHPADEYDSLAKSGRRVVDRLAGFGMLGPDSILVHGVHIDAGEAVLLAE
                     TQTWLTHQPRSNMNNAVGAADIESLLRLGIKVCLGTDGFSSTMWEEMKFAYLLQKVWQ
                     RDPRRMPGNEVVRMAAYHNAELAQRFFPDAPLGRLVPGAYADIILVNYHPYTPLTPGN
                     LPWQILFGFHESMVTTTIAAGKILMRDHQLLTMDEAAITARARELAPQVWENYQNLVP
                     AD"
     gene            complement(2012..2488)
                     /locus_tag="VN363_01690"
     CDS             complement(2012..2488)
                     /locus_tag="VN363_01690"
                     /inference="COORDINATES: protein motif:HMM:NF017517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008138 - protein
                     tyrosine/serine/threonine phosphatase activity [Evidence
                     IEA];
                     GO_process: GO:0016311 - dephosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dual specificity protein phosphatase family
                     protein"
                     /protein_id="HWQ83244.1"
                     /translation="MSALTRLPFDLPGAVYRSPMPLGPYDPDQELLKDYHAANIDLVV
                     MLSATGEDVRLTGLDLACLYQRAGFKILQLPIPDFSTPPREALAGVLEKIRAAALQGQ
                     NVVIHCSAGIGRTGLVSACLARQTLGLDGEAAIAWVRDIIPGAIETETQRQFVLAF"
     gene            complement(2493..>4405)
                     /locus_tag="VN363_01695"
     CDS             complement(2493..>4405)
                     /locus_tag="VN363_01695"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559891.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWQ83245.1"
                     /translation="VEYDLQPVISSAVQARQPESPSLHEKGNLLKHIKVRKGEIEQGF
                     AEADVILEHTFYTAITDHAFLEPECSVARLTQDGRMEIYVGSQIPYSDRAQVAAALGW
                     PDERVHIIGQLMGGGFGGKEDITGQIHAALLANVTRRPVKLLFDRHESLLVHPKRHAT
                     QIKVKMGAKQDGRLTAVETELYGDTGAYASLGEKVMTRATTHSSGPYEVPHVKADCYA
                     MYTNNPPAGAFRGFGVTQSAFAVETMMDMLAETLRMDRIDLRRQNALQVGSITNTGQV
                     LRESVGLSECIERTNQEMLRLGGERPFDPHPVPGAEHLVRSWGFAVAYKNTGLGGGAP
                     DKAGAEVELFHDGTLEVRTSSAELGQGLVTVLRMIVAEEFQLDPAAVKVLVMDTDLTP
                     DGGPTTASRQTYVTGNAARHAAQTLRSAMASALAEKYDLPPDQIRFVEGLARVNGHSL
                     PMGEVVQILKDEGREPLARYEYWAPETRPLGQGGDMHFAFAFAAQAAEVEVNTHTGEV
                     RVVRVIAANDVGRAINPLGLQGQVEGGIMMGIGNALTEHFIVEEGRVITDRLARYRIP
                     SITMTPAITSIVVEHPVASGPYGAKGVGEIVSIPTTPAITNAIYNAVGVRVDSLPVDQ
                     EAILLALRTAQP"
BASE COUNT          801 a         1366 c         1339 g          899 t
ORIGIN      
        1 gtgtttgttg atgctgatca gcagcgaggt cagtccttcc acgatcaggg cattctccag
       61 gctgccggca taataagcgt tcatcccggc ggcttcggcg agctgcatga ccacccgggc
      121 ggtttccagg tcatcggcgc aaaccagcac atccacgtcc agcttctcgc ccaggttctt
      181 tttcagggcg ctggcaggca cattctggaa agctgctgcc acgcgcacgc cggctcccag
      241 caggtcctgg gcgatacgcc cggcggaagg tgcagctggt gggtgcgggt cgcgaaaatc
      301 gacccgggcg gtcgtatcca ccaggatctt gccctggagg tcgtccttca ggctttccag
      361 cgcagcctgg tgagcggtgg cgaccacggt cagcacgcag atatccgcct ggcgtgcagc
      421 ttcgctgttt tccaggccgc gagcgcagtc gcgccccaat ttagcattca gctcggcagc
      481 agttgcttcc gctttttccg cctggcgcga gccaatcagg atcgggtagc cggcagacgc
      541 ccagcgcagg gccagaccgg ggccttcttt accggtgccg cccaggattg ccagagtggg
      601 aagcggattg cttgggctca taaattctcc atttttcagt cagcaggaac gagattttgg
      661 tagttttccc atacctgggg cgccagttcc cgggcacggg ccgtgattgc tgcctcatcc
      721 atggtcagca gctggtggtc gcgcatcagg atttttccgg cggcgatcgt ggtggtgacc
      781 atgctctcgt ggaagccaaa gaggatctgc cagggtagat ttccgggggt cagcggggtg
      841 taggggtggt aattcaccag gatgatatcc gcataggctc ccggtaccag cctgcccaat
      901 ggtgcatctg ggaagaagcg ctgcgccagc tcggcattgt gataggctgc catacggacc
      961 acttcattgc cgggcatccg gcgtggatcg cgctgccaca ccttttgcag caggtaagcg
     1021 aacttcattt cttcccacat ggtcgagctg aagccatccg tgcccagaca gactttgatt
     1081 cccaggcgca gcagggattc gatatccgcc gcgccaacgg cgttgttcat attcgagcgc
     1141 ggctggtggg tgagccaggt ctgtgtttcg gccagcagga cggcctcgcc ggcgtcgata
     1201 tgcaccccgt ggacgaggat gctgtcaggt ccgagcatgc caaagcctgc caggcgatct
     1261 accacccgcc ggccggattt cgccaggctg tcgtactcat cggcggggtg ttcagcgaca
     1321 tggatgtgga aaccggtgcc tttgggggca gcccggacac acttcgccag ggtctcatcc
     1381 gagagggtca gactggcgtg caggccaaac gttgctccca ggcgcgggtg gggagcttcc
     1441 ttcaaatggt tgataaaccg tacattctcg gcgatgccag cctgagattt ctccgcgcca
     1501 tcccgatcgg tgacttcata acacaagaca gcccgcaggc cggataggtc caccgcttcg
     1561 gcaatcgtat ccagcgaacc ggcgatggca ttgggtgagg cgtggtggtc gatcagcgtg
     1621 gtcgtgccat ggcggatcgc atcgaccagc atcacctcgg ccgaggcgcg cactgcatcc
     1681 atatccaggg ccttatccag gggccaccac agcttacgca ggatatccgg gaaatctttc
     1741 ggcgcggcac cggggattcc catgccgcgc gagaatgccc cataaaaatg ggtatgggcg
     1801 cagatattgc ccggcatcac atattgacca tgggcatcca gcacttcagc gtcaggatac
     1861 ctggccagca ggtccgcttg cggtccaatc tctgcgatgc gatcctcttt caggtatacc
     1921 gcctggcttt cgagcacccg gttttcgcgt tcccaggtga tcaggatgcc gttggtgatc
     1981 aggtattctg acatgacccc tcctcttact gctaaaaagc cagcacaaac tggcgctgtg
     2041 tctcagtctc aatggctccg gggatgatat ctcgcaccca ggcgatagct gcttccccat
     2101 ccaatcccag agtctgcctg gcgagacagg cagacaccag tcccgtccgc ccgatccctg
     2161 ctgagcagtg tatgaccaca ttctgaccct gaagggcggc agcccggatt ttttccaaca
     2221 cgccggccag tgcctcccgc ggtggggtgg aaaagtcagg gatggggagc tgcaggatct
     2281 taaaaccagc tcgctgatac agacaggcca gatcaagtcc ggtcaggcgg acatcttcgc
     2341 cggtcgctga gagcatcacc acgagatcaa tatttgccgc gtggtagtcc ttcagcagct
     2401 cctggtctgg atcgtaagga cccaacggca tcggactgcg gtacaccgcg ccgggcagat
     2461 cgaatggtag tctggtaaga gcgctcatcc tctcatggct gagctgtgcg cagtgccagc
     2521 aggatcgctt cctggtccac cggcaggctg tcgacgcgca cgcctaccgc attataaatc
     2581 gcatttgtga tcgccggtgt ggtcgggatg gaaacgatct cgcccactcc cttggcgccg
     2641 tacggccccg aagccaccgg atgttccacg acgatcgagg tgatcgcggg ggtcatggta
     2701 atcgatggga tgcggtagcg cgccagacga tcggtgatca cccggccttc ctcgacgatg
     2761 aaatgctcgg ttagggcgtt cccgatcccc atcatgatcc cgccttccac ctggccctgc
     2821 aggccgagtg gattgatcgc ccgaccgaca tcattcgccg cgatcacccg caccacgcgc
     2881 acttcgccgg tatgggtatt gacttcgacc tcggctgcct gggcagcgaa ggcaaaggca
     2941 aagtgcatat ctccgccctg gcccagcggc ctggtctctg gcgcccagta ttcgtagcgc
     3001 gccagcggct cgcgaccttc atctttcagg atctggacca cttcgcccat aggcaggctg
     3061 tgcccgttca cccgggccag cccctcgacg aagcggatct gatcgggcgg caggtcatat
     3121 ttttccgcca gggcgctggc catcgccgag cgcagcgttt gggcagcatg ccgggcggca
     3181 tttcctgtga cgtaggtctg gcgtgaggcg gtcgtcgggc cgccatccgg ggtgaggtca
     3241 gtgtccatca ccaggacttt gaccgcggct ggatcgagct ggaattcttc ggccacaatc
     3301 atacgcagca cggttaccag cccctggccc aactcggcag aggatgtgcg cacctccagc
     3361 gtgccgtcgt ggaacaattc gacctccgcg ccagccttat ctggagcgcc gccgcccagc
     3421 ccggtatttt tgtaggccac ggcaaaaccc caggagcgga ccagatgctc ggcaccggga
     3481 accgggtggg gatcaaaggg ccgctcgccg cccagccgca gcatttcctg gttggtgcgc
     3541 tcgatgcatt cactcaggcc aacgctttcg cgcagtacct gcccggtatt ggtaatgctg
     3601 cccacctgca gcgcattctg gcggcgcagg tcaatccggt ccatccgcag ggtctcagcg
     3661 agcatatcca tcatcgtttc gaccgcaaaa gccgactggg tcaccccaaa gccccggaaa
     3721 gcgcctgccg ggggattgtt ggtgtacatg gcatagcagt cggctttgac atgcggcact
     3781 tcataaggtc cggaggaatg cgtggtggcc cgggtcatca ctttttcacc gagtgaagcg
     3841 tacgccccgg tgtcgccgta cagctccgtc tccaccgcgg tcaggcggcc gtcctgtttg
     3901 gcgcccattt tgaccttgat ctgggtggcg tgccgcttgg gatgtaccag caggctctcg
     3961 tggcggtcaa agagcagctt caccggccgc ctggtgacat tcgccagcaa ggcggcgtgg
     4021 atctgaccgg tgatatcttc tttaccgcca aaaccaccgc ccataagctg cccgatgatg
     4081 tggacgcgct catccggcca acccagggcc gcggccacct gggcccggtc ggaataaggg
     4141 atttgcgagc ccacataaat ttccatgcgc ccatcctggg tgaggcgggc gacggagcat
     4201 tcgggttcca aaaaggcgtg gtcggtgatc gccgtgtaga aggtgtgctc gaggatgacg
     4261 tctgcttcgg caaagccctg ttctatctca cccttgcgca ctttgatatg cttgagcaaa
     4321 ttccccttct cgtgcaggct gggtgattca ggctggcgag cctgcaccgc gctgctgatc
     4381 accggctgca gatcgtattc cactt
//
LOCUS       DATMXK010000092         3793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_133257, whole genome shotgun sequence.
ACCESSION   DATMXK010000092 DATMXK010000000
VERSION     DATMXK010000092.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133257"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..814)
                     /locus_tag="VN363_01700"
     CDS             complement(<1..814)
                     /locus_tag="VN363_01700"
                     /inference="COORDINATES: protein motif:HMM:NF012981.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWQ83246.1"
                     /translation="MPRYQIIVNPTSGRGAGLASIPLIRQNLDAQGDAQGDAQGDAQG
                     IDYTITQTERPWHAAELTQKAVQEGYDVVVSVGGDGTANEVINGLVLAQKAGLGTAAM
                     GVLAVGRGNDFAYSMYIPGGIEAGCQTLVHGQRKLIDIGLVRGGDYPEGRYFGNGVGI
                     GFDAVVGFVAVKMTRLTGFASYIAAVVKTIFLYFDAPTVKIETDRETLTLPALMVSIM
                     NGRRLGGGFRLAPQGDNADGLMDLTIARQVSRGRVLTLILHFLKGTQATQKEI"
     gene            985..2025
                     /locus_tag="VN363_01705"
     CDS             985..2025
                     /locus_tag="VN363_01705"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase"
                     /protein_id="HWQ83247.1"
                     /translation="MQSDTIPDLVYFDPIGHEAHYAAGHPERPERIEAIRQGLQSQGL
                     WEPYPHLAPRQIPREILEKVHTPQYLRQLENACWGGEWLDMDTYTSPASWVLALNAAG
                     GALAVAETVWQSQAWRGFALARPPGHHACSDRGMGFCLLNNVAIAAEGLIQAQGARRL
                     AIVDLDLHHGNGTQEIFYERSDVFYISTHQTPLFPGSGAIEEMGFRAGQGFTANLPLP
                     PGAGDSAFSAGMEHFILPLLEKYHPEALLVSIGFDVHWRDPLGSLQLSAEGQYRLIRS
                     LTQFADLHCAGHIALFLEGGYDLQASSVCAQASVAGLLNLPFHDLLGPAPGEEGEHWQ
                     VWLERARKQWGL"
     gene            2416..>3793
                     /locus_tag="VN363_01710"
     CDS             2416..>3793
                     /locus_tag="VN363_01710"
                     /inference="COORDINATES: protein motif:HMM:NF014045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NYN domain-containing protein"
                     /protein_id="HWQ83248.1"
                     /translation="MTSQLAVFIDFENVALWAEREFFDFELTPLMEYLQARGPAVVKR
                     AYGDWSRFSRYRDELMNHSIDLIQIFSVRAGKNRTDIRMAIDAMEVAMHRPQIDTFVI
                     VSGDSDFGPLVAKLREYGRYTLGIGPRSVTHHLLVKSCDEFVYLETVMGETIITGDHT
                     SAELDTARSLLRKALQVHGQRGDIPVLATRLKQTMLLMDPAFNEANFGYSQFKAWLED
                     SRDHIKLFLKDLQLFVAPLDYQAAGYNEFGADSAGMELVEKVEAGSAVLEDQYRQLFA
                     RLRLTSVDLETRRDVLRDVYREVSEHPYELSVDDLLETLDERYEEQGLMRSKANLREI
                     IQMAYRQNAFTFDGQPPSQFNPVNLAKGIETEAEFISWSEEDFIFALVRAGVELDIDV
                     LANLMLNDSAQADYVQCLLDDLHARGMIIRRGKHYVIAEPSSIPFRDDPALQVLMDDL
                     QQVEVPDGM"
BASE COUNT          811 a         1072 c         1058 g          852 t
ORIGIN      
        1 ggatctcttt ctgggttgcc tgggtgcctt tgagaaagtg gagaatgagg gtcaacacgc
       61 gtccccggct gacctgccgg gcgatggtca ggtccattaa tccatccgca ttatcgcctt
      121 gcggtgccag cctaaaacca ccccccaacc ggcggccatt catgatcgag accatcaagg
      181 cgggtaaggt cagggtttca cgatctgttt cgattttcac ggtaggagcg tcgaaataca
      241 ggaaaatggt tttgacgact gcggcgatgt aggaagcgaa gccggtcagc cgggtcattt
      301 tgaccgccac aaacccgacc acagcatcga atccaatccc caccccgttg ccaaaatagc
      361 gtccttcagg atagtcaccg ccgcgcacca gaccgatatc gatcagcttg cgctgcccgt
      421 gcaccagtgt ctggcagcct gcctcaattc cacccggaat atacatgctg taggcgaaat
      481 cattgcccct gcctactgcc agcacaccca tcgcggctgt gcccagcccg gctttctggg
      541 cgagcaccag cccattgatc acttcgttag ctgtcccatc cccgcccaca ctgacgacca
      601 catcataacc ttcctggaca gccttttggg tcagctcggc agcatgccag gggcgttctg
      661 tctgggtgat cgtgtaatca ataccctggg cgtccccctg ggcgtccccc tgggcgtcac
      721 cctgggcgtc cagattctgg cgaattaaag ggatggaggc caggccagct ccccgcccgg
      781 aggtaggatt gacaatgatc tgatagcgag gcataactgg tttctccacg agagttattc
      841 aaaattacga ccgtcatagt ttactatagt ccggttaaat aaatggctgt ttttttacga
      901 ttggaacagg cttgccaggc atgatttaga aacggccttg acaggaatct aatccattcg
      961 ggcagctttc tggtataaat actcatgcag tccgatacca ttcccgatct ggtgtatttc
     1021 gatcctatcg gccacgaagc tcactatgct gcgggtcacc ccgagcggcc tgagcgcatc
     1081 gaggcgatcc gccagggctt gcagtcccag ggcttatggg agccttaccc gcacctggcg
     1141 ccgcgtcaga tacctcgcga aattttggaa aaagtacata ctccgcagta cctgcgccag
     1201 ctggagaacg cctgctgggg aggcgagtgg ctggatatgg acacatatac ctctcctgct
     1261 tcctgggtgc tggcattaaa tgctgccggc ggcgcactgg cagtggccga gactgtctgg
     1321 cagtcccagg cgtggcgcgg tttcgccctg gcgcgcccac ccggtcacca tgcctgctcg
     1381 gatcgcggca tgggcttttg cctgctgaac aatgtcgcga tcgctgccga aggcctgatc
     1441 caggcacagg gcgccaggcg cctggcgatc gtggacctcg acctgcacca tggcaatggc
     1501 acgcaggaga tcttctacga gcgcagcgat gtattctata tctccaccca ccagacgccg
     1561 ctctttcctg gctccggcgc aatcgaggag atgggcttcc gggcgggaca gggctttacc
     1621 gccaatttgc cgctgccgcc aggggctggc gacagcgcat tcagcgccgg aatggagcat
     1681 ttcattctac ccctcctgga gaaataccat cctgaggcgc tgttggtcag tattggcttc
     1741 gatgtccact ggcgcgaccc gctgggcagc ctgcagcttt cggctgaagg ccagtaccgg
     1801 ctgatccgga gcctgaccca atttgccgac ctgcactgcg cagggcatat agccctgttc
     1861 ctggaaggcg gctacgacct gcaggcctcc tcggtgtgtg cccaggcgag tgtggcgggt
     1921 ttgttgaatc tacccttcca cgatctgctc ggccctgccc cgggtgagga aggcgagcat
     1981 tggcaggtct ggttagaacg cgctcggaaa cagtgggggt tataagccag ggattgcatc
     2041 tggcaaaagg ttgctttatt caaagaattg cagtaaagtt agattaaatc atcgccctgc
     2101 atttccgatc actacgcacc gcgcgacgtt agccggcaaa ataaccggat aatcctcaaa
     2161 cgagatcagt gaagccacga agtaacatcg aaataaagaa caatttacaa gcggccttct
     2221 gaagcactcg ttaagcgtct ggtaagggta tctcgtttag tacgtcctcc cgacctgcgc
     2281 ttaagatcgt ccatgaggat aaccggtatg agagcctgga acgtaaacga cacaagtttc
     2341 cgtctggtgt ctgggattcc catcacagat gtgtgggaaa tgcgggggat agaaaataaa
     2401 agatcggagt accctatgac cagccagctg gcggttttta ttgatttcga aaatgttgct
     2461 ctgtgggcgg aacgcgagtt ttttgatttt gaattgaccc ctcttatgga atatttacag
     2521 gcgcgtggcc cggccgttgt caagcgcgcc tacggtgatt ggtcgcgttt ttcacgctac
     2581 cgtgacgagc tgatgaacca ttccatcgac ctcatccaga tcttcagcgt gcgcgccggt
     2641 aaaaatcgca ccgatattcg catggcgatt gatgccatgg aagtcgccat gcaccgcccg
     2701 caaatcgata cctttgtgat tgtctctggc gacagcgatt ttggtcccct ggtggcaaag
     2761 ctgcgcgaat atgggcgcta caccctcgga atcggcccgc gctccgtcac ccaccacctt
     2821 ttagtgaagt cgtgcgacga gttcgtgtac ctggaaacgg tgatgggcga gaccattatc
     2881 accggcgacc atacctctgc tgagctggat actgcccgca gcctgctgag aaaagccttg
     2941 caggtccatg gtcagcgtgg cgatatcccc gtgctggcga cccggctgaa gcaaaccatg
     3001 ctgctgatgg atccggcttt caatgaggct aatttcggct attcccaatt caaagcctgg
     3061 ctggaagaca gccgcgatca tatcaagctt ttcctcaagg atctgcagct ctttgttgct
     3121 cccctggatt accaggctgc cggctacaac gagttcggtg cggacagcgc cggaatggag
     3181 ctggtcgaga aagtcgaggc cgggagtgct gttctggaag accagtaccg ccagttgttt
     3241 gcccgcttgc gccttacctc agtcgatctg gaaacccgcc gtgatgttct ccgcgatgtt
     3301 tatcgggagg taagcgagca tccctatgag ctgagcgtgg atgatctgct tgaaacactg
     3361 gatgaacgct acgaagagca gggcttaatg cgcagcaaag cgaacctgcg tgagattatt
     3421 cagatggcgt accgccagaa tgcatttaca ttcgatggtc agcccccttc ccaatttaat
     3481 ccggttaacc tggcgaaagg catcgaaacg gaagcggaat ttatcagctg gtccgaagaa
     3541 gattttatct ttgcactggt gcgcgccggt gtggagctgg atatcgatgt tctggccaat
     3601 ttgatgctca atgacagcgc ccaggccgac tacgtacaat gcctgctgga tgatctccat
     3661 gcccgcggta tgatcatccg gcggggtaag cattacgtga ttgccgaacc cagcagcatc
     3721 cccttccgcg atgatcctgc tctgcaggtc ctgatggatg acctgcagca ggtggaagtg
     3781 ccggatggaa tgc
//
LOCUS       DATMXK010000093         4377 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_98311, whole genome shotgun sequence.
ACCESSION   DATMXK010000093 DATMXK010000000
VERSION     DATMXK010000093.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4377)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4377)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4377
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98311"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..750)
                     /locus_tag="VN363_01715"
     CDS             complement(<1..750)
                     /locus_tag="VN363_01715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83249.1"
                     /translation="MNRIQRTDLLRHARITAWILFLVCLPVTSFPYFPAAVGGTALVR
                     PLSIYPLIVLLVLVTIPRLWKLRLPRTFLSLLPFVIIAVVSSLFSLFRGIEPGLNVTV
                     GERVLRGLITLGLGVSFYLTTSLTPRSLDDLKSTLRWLYAGFGIALIWGSLQAIYVVH
                     FTKAWYRIMNNLQQYISSRRLIQNRISGMTYEPNWFAEQITGLLLPWLLASVLTGYTV
                     FKWRRGWLTIEWFLLVWSVALLPLTFSRAGVL"
     gene            complement(747..1805)
                     /locus_tag="VN363_01720"
     CDS             complement(747..1805)
                     /locus_tag="VN363_01720"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83250.1"
                     /translation="MQEWSLRNEIYTALHRWPLILLYGLVGCSLGVLLSFIWPSPWRA
                     TAEIYVGMEPYRWTEDENVVAYALGTRFNYADDYKNWQMANLDALVRMDEITLKTLDR
                     LRQSDDFWSDINRQGLADMLQVYWRNPGRWRLAADHTDPDIASAAALAWREVVYETVH
                     YSIGFAQDTFRLDIQLKAIQNQLVPLTTQRAELETALAGLTDIQNELENFSASQAVQE
                     LTRWQMASWASHASQNDPIWVTLLNDMPVEGQTRAMYLAWLDRARITIGQDLLTLQSQ
                     IDTLSSQQAQAMAEYAAASQASRGLSANLQLEKISDTNPKPVALRPTAQMALVGTIIG
                     VLVWALLWLARLTLRKTG"
     gene            complement(1820..2779)
                     /locus_tag="VN363_01725"
     CDS             complement(1820..2779)
                     /locus_tag="VN363_01725"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWQ83251.1"
                     /translation="MGNNRTPDPRSLPTSSAEQITGETPTLSICIVSLNSWSYLQDCL
                     QSLAENTRTDYEVIITDNGSTDGTQSMLAAKFPGVRLIQNQHNEGYTRPMNQALQLAQ
                     GRFLAQLNPDTLILPGAMDHLISFLDAYPQAGICGPKVLNRDHTLQKPCRRGEPRPWA
                     VICYFLGLSALFPKIRLFGEYLMSYKDEDETHTVAGVSGSCMLIRRDVVNSIGFLDER
                     FFAYQEDADYCFRARQAGWKIYYHPQAQIIHYGGMGGSRVQPYRSIYEWHRSYFLYYR
                     KNLARDYFFLFNWLYYVAMGVKLILSLGLNFIRRDKFAGSRKP"
     gene            complement(2719..3864)
                     /locus_tag="VN363_01730"
     CDS             complement(2719..3864)
                     /locus_tag="VN363_01730"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 1 protein"
                     /protein_id="HWQ83252.1"
                     /translation="MTQKTHLRVGIDATALPPQPVGAGNYMIQLIRALAKLDSDLELV
                     IFAHPHGKALIDVPEQAGLHWSLVPDQKPALRLVWEQTRFPGLIRQEGIDLLHSLHYT
                     RPLRLPCTSVVTFHDMTFFLFPELHTRAKRLFFPAAIRYSARTADAVIAISESTRQDA
                     IRLLKIDPARIQTIPLGVTGEFKPISASPQLSIIRQKYNLPECFILYVGLLEPRKNLP
                     LLLRAYRQVLDRGPAPVLVLAGRMGWTVDTLRHTIDDLALTDQIHFTGYVTPDDLPFV
                     YNLADLFVYPSLYEGFGLPPLEALACGTPVITSSVSSIPANMGDAAILVQPDDQNALS
                     TAIREILDNPELRQELIVKGPIQASKFKWETTARQTLAVYQQVLQNK"
     gene            complement(3882..>4377)
                     /locus_tag="VN363_01735"
     CDS             complement(3882..>4377)
                     /locus_tag="VN363_01735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83253.1"
                     /translation="YGRLRFANKLLGDRYRRRFNRGYLLEDCLRLFLAFVRLRASQVS
                     AILTGRRQFWQTADLPAARESLQKRRQVADESLFALQKTFPSPLIWGGAPVLTWDIIQ
                     SVYLPLIQAQETRPLPEFPASYRITIPPSSSLQRWNRIRQLEGTVMLFQHIYRAVQRR
                     LAIP"
BASE COUNT         1062 a         1087 c         1267 g          961 t
ORIGIN      
        1 taataccccg gctcgtgaga aggttaaagg cagtaaggcg acactccaga ccaacaagaa
       61 ccattcgatc gtcagccagc cgcgccgcca tttaaacacg gtataaccgg tgagtaccga
      121 tgccagcagc catggcagta acaaaccggt gatttgctcg gcgaaccagt tcggctcgta
      181 tgtcatacca gagatacggt tctggatcag gcgccgggaa gaaatatatt gctggaggtt
      241 attcatgatc cggtaccagg ctttggtgaa atggactaca taaatagcct gcagtgatcc
      301 ccaaatcagg gcaatcccaa atccggcata cagccagcgc aaggtgcttt tcagatcatc
      361 caaactgcgc ggggtcaggc tggtggtgag atagaacgag acccccaatc ccagagtgat
      421 caggccccgt aagacgcgct cgccaaccgt gacattgagg cctggctcga ttcctctgaa
      481 cagtgaaaat aacgagctga ccacggcgat gatcacaaat ggcagcagcg atagaaaagt
      541 acgcggcaag cgcagtttcc acaggcgtgg gatcgttacc aacaccagca gcacaattaa
      601 tgggtagatc gacagcgggc gcacaagcgc agtaccaccg actgcggcag ggaaataagg
      661 gaagctggtt acaggtaaac agaccaggaa aaggatccag gctgtgatgc gtgcatggcg
      721 aagcaaatct gtgcgctgaa tgcggttcat ccagtttttc tcaatgtcag gcgagcaagc
      781 cagagcagtg cccagaccag aacaccgatg atcgttccga ctaatgccat ctgagctgtg
      841 ggacgcaggg caactggttt ggggtttgta tctgagatct tttccagctg cagatttgcc
      901 gagagacccc ggctggcttg ggatgcagct gcatactcag ccatcgcctg ggcttgctgg
      961 ctggaaaggg tatcgatttg agactgcagg gtcaggaggt cctgtccaat ggtgatgcgg
     1021 gcgcgatcca gccaggccag atacattgcg cgggtttgac cttccactgg catatcattt
     1081 aataacgtaa cccagatcgg atcattttga gatgcatggg atgcccagga tgccatttgc
     1141 cagcgagtaa gctcctgcac agcctggctt gcggagaagt tttccagctc attttgtatg
     1201 tctgtgagcc cagccagggc ggtttccagc tcggcacgct gcgtggtgag ggggaccagc
     1261 tgattttgaa tggctttgag ctggatatcc agccggaagg tatcctgagc gaatccaatc
     1321 gaatagtgga cagtctcgta gacgacctcg cgccaggcga gggcagctgc tgaagcgata
     1381 tccgggtctg tatggtcggc tgcgaggcgc cagcgcccgg ggttacgcca atagacctgt
     1441 aacatatctg ccaacccctg gcggttgata tcactccaga aatcatcgct ttgccgcagc
     1501 cgatccaggg ttttaagggt aatttcgtcc atcctcacca gagcatcgag gttagccatt
     1561 tgccagtttt tatagtcatc ggcatagtta aaccgcgtac ccagggcata agccaccaca
     1621 ttttcgtcct ctgtccagcg atagggctcc atgccaacgt agatctctgc agtagcgcgc
     1681 cagggtgaag gccagataaa ggagagcagg acccccaggc tgcagcctac caacccatat
     1741 aacaagatca acggccagcg gtgcagggcg gtatagattt cattgcgtaa cgaccattct
     1801 tgcatgcagg tactccaatt cagggtttgc gcgagccggc aaatttatct ctgcggataa
     1861 agttcagtcc taaggacagg ataagtttca cacccattgc cacgtaatac aaccaattaa
     1921 agaggaaaaa ataatcccgg gcaaggttct tgcggtagta caggaaatac gagcgatgcc
     1981 attcgtagat tgagcggtaa ggttggacgc gtgaaccgcc catgccgccg tagtgaatga
     2041 tctgagcctg gggatgataa taaattttcc agcctgcctg gcgtgctcga aaacaataat
     2101 cggcgtcttc ctggtatgca aaaaatcgct catcgagaaa gcctatgctg ttcactacat
     2161 ctcgacggat aagcatgcac gagccggata cgccagccac ggtgtgggtc tcatcctcat
     2221 ctttgtaact catcaagtat tcaccgaaca ggcggatttt ggggaaaagg gcggacagcc
     2281 ccaggaagta acatatcact gcccaggggc ggggctcacc tcgccggcag ggtttttgca
     2341 aggtgtgatc gcggtttagt acttttggtc cgcaaatgcc agcctggggg tacgcatcca
     2401 gaaaagaaat caaatgatcc attgcgccag gcaggattaa ggtatccggg tttaattgag
     2461 ccaggaaacg gccttgcgcc agctgcagcg cctggttcat tgggcgggtg tagccttcgt
     2521 tgtgttgatt ttggatcagc ctgacgccgg gaaattttgc cgccaacatg gactgagtgc
     2581 catcggtgga gccattatcg gtgatgatga cctcataatc tgtgcgggta ttctctgcca
     2641 gagactgcaa acaatcctgg agataactcc agctgttcag gctcacgatg cagatcgata
     2701 gagttggagt ctccccggtt atttgttctg cagaacttgt tggtagactg cgagggtctg
     2761 gcgtgcggtt gtttcccatt taaattttga tgcctggatt ggacctttga cgattagttc
     2821 ctggcgtaat tctgggttgt ctagaatttc ccgtatggcg gtgcttaagg cattctgatc
     2881 gtcgggctgc acaaggattg ccgcatcacc catattagct ggtatggatg ataccgagct
     2941 ggtgattacg ggcgtcccgc atgccagcgc ttccagaggc ggcagtccaa acccttcata
     3001 cagcgatgga tagacaaata aatctgccag attataaacg aagggcaggt catctggggt
     3061 cacatatcct gtgaagtgaa tctgatcagt caaagccaga tcatcgatcg tatgccttag
     3121 tgtgtcaacc gtccagccca tccgcccggc gaggaccagc acaggagccg ggccacgatc
     3181 cagcacctgc cggtatgcac gcaaaagaag gggaaggttc ttccgtggtt ccaataagcc
     3241 gacatagagg atgaaacatt ccgggaggtt atatttctgg cggattattg aaagctgagg
     3301 tgacgcggag attggcttga actcgccggt tacaccaagc ggtatcgttt gaatgcgggc
     3361 aggatcgatc ttcagcaggc ggatggcatc ctgtcgcgtg ctctccgaga tcgcgataac
     3421 cgcatcggct gtgcgagcgc tgtagcgaat cgcggccggg aaaaacaaac gtttcgccct
     3481 ggtatgcagc tcgggaaata agaagaaggt catgtcatgg aaagtcacta ccgaagtgca
     3541 cggtaaacgg agtgggcgcg tgtaatgtaa cgaatgcagc agatcgatgc cttcctggcg
     3601 gatgaggccc ggaaagcggg tctgttccca gaccaggcgt aaagcaggct tctgatcggg
     3661 taccaggctc cagtggagac cggcctgttc ggggacatcg attaaagctt ttccgtgagg
     3721 atgggcaaag atcaccagct ccaggtctga gtcgagcttt gccagggcgc ggatcagctg
     3781 gatcatataa ttgcctgctc ccacaggctg gggaggcagg gcggtggcat cgatgcctac
     3841 acggagatga gtcttttgag tcatggctgg gtaagtctgg tttacgggat tgccaggcgc
     3901 cgttgaacgg cacggtaaat atgttggaag agcatgacgg tcccttcaag ctggcggatg
     3961 cggttccagc gctgaaggct gcttgatggg ggtatggtaa tccggtagct ggcaggaaat
     4021 tcgggcagcg gccgggtttc ctgggcctgg atcagcggta aatagacgga ttgaataata
     4081 tcccaggtca gtaccggcgc gcctccccaa atcaggggtg aggggaaggt tttttggaga
     4141 gcaaataaac tttcatcggc gacttgccgg cgtttctgaa gactttcgcg tgctgccgga
     4201 agatcagctg tctgccaaaa ttgtcttctg ccggtcaaga tcgcgctcac ctgtgaggcg
     4261 cgcagccgca caaacgccag gaacagtctc aagcagtctt ccaaaagata gccccggttg
     4321 aaccggcggc gatagcgatc tccaagcaat ttattggcaa agcgcagtct tccatag
//
LOCUS       DATMXK010000094         2563 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_6833, whole genome shotgun sequence.
ACCESSION   DATMXK010000094 DATMXK010000000
VERSION     DATMXK010000094.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2563)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2563)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2563
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6833"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2563
                     /locus_tag="VN363_01740"
     CDS             <1..>2563
                     /locus_tag="VN363_01740"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWQ83254.1"
                     /translation="LYFNQPMDRASVESALNTPVRGSLTWLDDSTLSFAPAELLQPAS
                     QVSLSLEETARSAQGQPLTAPVRLEYQTTGNLELVQRLPTPDINEVDPSSPIVAAFNH
                     PVVALGAEADSLPPAFTLEPAAAGRGEWLNTSTYIFYPRPALLGGAAYTVQVDPTLTS
                     TAGSPLGGETDWVFYTAAPRILGVTPSSQDSPVIRLDSAFELAFNQPMDPASTEAAFR
                     LRTSNGENVTGSFSWNDDLTMLTFTPAALLGRSLGLELTVRGSAQGAGSTPLEIPFVA
                     LYSTYPTLVVNSSNPGPAGVIAPDRNIAINLSAPVAEDTVKDALTITPAVPNLSTWWN
                     SWDLSLNISGSFAPNTDYQLSLSTALQDEWGGSLAQPFTLPFRTGAVSPNFTMPLGSN
                     VLFLSSTDRTLPARATNIFNASISLGAITLDDFFSMVRSGGYQVIESFQPARPLTWSQ
                     ALNTQSDVNETVQIQLNPGGTPLEPGLYFMRLSFNQPGVTGGPFLVVVGDSQLTYKSS
                     PSDVLLWATSLEDFSPRSGAAVTVYDENGAVLAAGQTRADGVFHSAIPVQADIYLETI
                     AVAGQPGEAGFSLALGTWNQGLNAWDFDIPSDSRPPGLRTYLYSDRPIYRPGDTIYFR
                     AIVRQAQNGRYSLPDRSTVSLKLFDGLGIEVAAFDLQLSAFGTAHAEYTLPQGAATGY
                     YNLVVEDSFYDTLSIQVADYRKPEINLQVTLDQAEILSSAGFNAAINARYFFDAPAGS
                     LPVQWTLYAAPQDFYVPGYQTGALDFSWMQAFNFPSFGGSLGIQVAQGASETAPDGTL
                     TLEFPTLPENSAVNPQLRQRYTLEVVAADESGLPVAARASLLANPAEFYIGLRPDT"
BASE COUNT          449 a          920 c          663 g          531 t
ORIGIN      
        1 ccctgtattt caaccagccc atggatcgcg cttcggtcga aagcgccctg aatacccctg
       61 tgcgcggcag cctgacctgg ctggacgact ccacccttag ctttgcccct gctgagctgc
      121 tccagcccgc cagccaggtt tccctcagcc tggaagaaac cgcccgcagt gctcaggggc
      181 agcccctcac cgcgccggtc cgcctggagt atcaaactac cggcaacctg gagctggttc
      241 aacgcctgcc caccccggat attaacgagg tcgatcccag ctccccgatc gtcgccgcct
      301 tcaatcatcc cgtggtcgcc ctgggcgccg aagcagacag cctcccgcct gcctttacac
      361 ttgagccggc tgccgcggga cgcggtgaat ggctgaacac cagtacctat attttctatc
      421 cccggccagc cttgctcggc ggggctgctt ataccgtcca ggttgacccg accctgacca
      481 gcacggctgg cagcccgttg ggcggtgaga ccgattgggt cttttatacc gccgcgcccc
      541 gcattcttgg cgtcacccct tcatcccagg attcacccgt aatccgcctg gacagcgctt
      601 ttgagctggc attcaaccag cccatggatc cagcttccac ggaagccgcc ttccggctgc
      661 gcacctccaa tggcgaaaac gtcaccggca gcttctcctg gaatgatgac ctcacaatgc
      721 tgacctttac ccccgctgcc ctgctggggc gtagcctggg tctggagctg accgtgcgcg
      781 gctctgccca gggggccggc agcactccgc tcgaaattcc attcgtggcc ctctattcga
      841 cctatccaac cctcgtcgtg aacagctcca accctggtcc ggcgggcgtg atcgctccgg
      901 accgtaatat cgccattaat ctctcggcgc ctgtcgccga agacacggtg aaagatgctc
      961 tgaccatcac acctgctgtg cccaacctca gcacctggtg gaacagctgg gatctttcct
     1021 taaatattag cggctcattt gcccccaaca ccgattacca gctcagcctg tccaccgctt
     1081 tgcaggacga atggggcgga tcgctggctc agccttttac cctgcccttc cgcaccgggg
     1141 ccgtatctcc caatttcacc atgcccctgg gcagcaatgt gcttttcctc tccagcacag
     1201 atcgcaccct gcctgcccgc gccaccaaca tcttcaatgc cagtatcagc ctgggtgcta
     1261 tcaccctgga tgacttcttc tccatggtcc gttcaggcgg ataccaggtc attgaaagct
     1321 tccagcctgc ccggcctctc acctggtctc aggctttgaa tacccaatcc gatgtcaacg
     1381 aaactgtcca gatccagctc aatcccggcg ggacgccgct cgaacccggg ctgtacttta
     1441 tgcgcttgag cttcaatcag cccggggtta caggcggtcc gttcctggtc gtggtgggcg
     1501 acagccagct tacctataaa tccagcccca gcgatgtgct gctctgggcg accagcctgg
     1561 aagatttcag cccgcgctcc ggcgccgcgg tcaccgtcta cgatgaaaac ggggctgtgc
     1621 tcgccgccgg gcagacccgg gcggatggcg tatttcacag cgcgatcccg gtccaggccg
     1681 atatctatct tgaaaccatc gccgttgcag gccagcccgg cgaagccggt ttcagcctgg
     1741 ctcttggcac ctggaaccag ggcctgaacg cctgggattt cgatatcccc agcgattccc
     1801 gcccaccggg gctgcgcacc tacctgtaca gcgaccgccc gatctaccgc cccggcgata
     1861 ccatttactt ccgtgccatc gtccgccagg ctcaaaatgg ccgttacagc ctgcccgacc
     1921 gctccaccgt gagcctgaag ctctttgacg gcctgggtat cgaggtggct gccttcgatc
     1981 tgcagttatc cgccttcggc accgcccatg ccgaatatac cctgccccag ggtgcggcta
     2041 ctggctacta taacctggtc gttgaggata gtttttacga taccctctcc atccaggtcg
     2101 ccgactatcg caagcccgag attaacctgc aggttaccct ggaccaggcg gaaatcctct
     2161 ccagcgccgg ttttaacgct gccatcaatg cccgctattt cttcgacgcc cccgccggca
     2221 gcctgcccgt ccagtggaca ctatatgccg ccccccagga tttctacgtc cctggctacc
     2281 agaccggcgc cctcgatttt agctggatgc aggctttcaa tttcccatcc ttcggcgggt
     2341 cgctgggcat ccaggttgcc cagggtgctt cggagacggc gcccgatggg accctgaccc
     2401 tcgaattccc caccctgccg gaaaacagcg ctgtaaatcc gcagttacgc cagcgttaca
     2461 ccctcgaagt cgtggctgcc gatgagagtg gtctgccggt agccgcccgc gccagcctgc
     2521 tcgccaaccc ggctgagttt tacatcggtc tgcgccccga cac
//
LOCUS       DATMXK010000095         3873 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_133108, whole genome shotgun sequence.
ACCESSION   DATMXK010000095 DATMXK010000000
VERSION     DATMXK010000095.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3873)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3873)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3873
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133108"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(253..936)
                     /locus_tag="VN363_01745"
     CDS             complement(253..936)
                     /locus_tag="VN363_01745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83255.1"
                     /translation="MSIRILIADDHSLIRAGLRALLQGTSDITVVGEVDDGISVLGQV
                     AALEPDIVLMDISLPGVSGIEATRQLRLISPRTRVLALTVHEDEAMLREMLRAGAYGY
                     ILKRADDSELIKAIRFVSQGDMYIYPSLTSALMKDFSPHGKAERDTEPTLTMREIDVL
                     LLLARGYTNRQIAEELHLSPRTVEGHRSNLVSKLGLKSRVELMNYVEEHNLAKKKLEK
                     SQKPKNPEA"
     gene            complement(911..>3873)
                     /locus_tag="VN363_01750"
     CDS             complement(911..>3873)
                     /locus_tag="VN363_01750"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWQ83256.1"
                     /translation="IPLLYFFHFRPLFNQVSERIRSEELLSRVLENLPVGVWITDAQG
                     NILHGNQASLKIWSGARYVSVDQYGEYKAWRLDNGKLVEHADWAAARSIRSGESIPDE
                     ELEIECFDGTHKIILNSATPFFEGQTLQGVIAINQDISERKRAEQALVKSEARLKTAF
                     DVLPVGAWMTDENGNILYGNPAGQQIWAGAKYVGIDQFGEYKAWWVSTGLPVEPEDWA
                     VARAVHNGETSWNEELEIECFDGTHKLIFNTAIPVRDDHGQVHGVFVVNEDITLRKQA
                     EQDLIRSNELIGKAFNSIDILIAYMDRDFNFIKVNEAYATSDGHPVAYYEGKNHFDLF
                     PNLENQAIFQKVVDTGEPYSVLEKPFEYAANPERGISYWNWRLQPVFELDGSVQGVVL
                     SLVDVTERKRAELLLKRQNQELIALSSAEAKQRKLAESLVQTMLAVNASLELEEVLKT
                     ILDQIHEAIEYQRANILLIDNGIFRIVLQHDLSEDKVLTDAVSGSSELADFPLLEVMR
                     TTHQPLLVEDTVETPDLYRVPGLPLPRTLLAVPLIVGSRLTGVINLTSEQPGVFNPET
                     ARRLMVFAAPAALAMENARLYAAELQARQLAETLNEVSRALSETLNFEKVMNTLLEYV
                     CHLVPVDQAFIGVCENESSLTLLAIRGYDLQINPKMELKDTFDSWDQPYLHEVLSSHK
                     SLLIPDTRLYPDWTTLVSQLPIRSSLAIPILVNGEAMGVLAMGNSKPYKINQEHVWLA
                     EVVIAQAAVSIQNAWLFEQVRSGHERLQSLSRRLVEVQESERRYIARELHDEAGQSLT
                     ALKFGLSLLEQDAEKPESQLLRIAELKQLTDSVSEELHRLAMDLRPASLDYLGLVVAL
                     EQLITGYAARYPMNIRFKEIGFANKVRLADYVETNIYRIVQEALTNAVRHAHASNVDV
                     IVEKRVDKVVVMIEDDGVGFETSGIRKSGHLGLLGIQERTQMIAGSLQIESKPGGGTT
                     IVVEVPNVDSHFDR"
BASE COUNT          864 a         1095 c         1003 g          911 t
ORIGIN      
        1 aacctatccc gagaagaaaa ttagtttgca ttaatgcaaa cttaatatag cggttttcag
       61 aagtgtgaac aagcgcactt atttacggat ttttctgcaa tgttctgcgt gaggaatacg
      121 gcgggatggc gatagaaata cccggtagat accgcgtagg aatcgtttaa caattacaaa
      181 ccggtcgcgt atatactacg caaccggttt gtttcaaccg gctacatgcc gccaggcagg
      241 aaaatttcgg ggttaagctt cggggttttt aggtttttga gatttttcga gttttttctt
      301 tgccagattg tgctcttcaa cgtaattcat taactcgacc cggctcttta aaccgagttt
      361 gctcaccagg ttggagcggt gaccttcaac cgtgcgcggg ctgaggtgca gctcttcggc
      421 tatctgccga ttggtgtaac cccgcgccag gagaagcaag acgtcgatct ctctcatcgt
      481 cagggttggc tcggtatccc gctctgcttt gccatgcgga gaaaaatctt tcatcagggc
      541 gctggtcagc gaagggtaga tgtacatatc cccttgcgag acaaagcgga tcgccttgat
      601 cagctccgaa tcatccgctc gcttgaggat gtagccgtac gccccggcgc gcagcatctc
      661 gcgcagcatc gcctcatctt cgtggacggt gagcgccagc acccgcgttc ggggggagat
      721 caggcgcagc tggcgagtgg cttcgatgcc gctcacgcct ggcaggctga tatccatcag
      781 gacgatatcc ggctccaacg ccgccacctg gcccagcacg ctgatgccat cgtcaacttc
      841 gccgaccacg gtgatatcgc tggtcccttg caggagggcg cgcaatcccg cccggatcag
      901 gctgtgatca tcagcgatca aaatgcgaat cgacattcgg tacctccaca acaatcgtcg
      961 tcccaccccc gggtttgctc tcgatctgta agctgccggc aatcatctgg gtgcgttcct
     1021 gaatacccag caatcccagg tgcccactct tgcgtatccc ggaggtctca aagcctacac
     1081 catcgtcttc gatcatcacc acaactttat ccacccgctt ttccacgatc acatccacat
     1141 tgctggcatg cgcgtggcgg actgcgttgg tcagcgcctc ctgcacaatc cggtaaatat
     1201 tggtctcgac ataatctgcg aggcgcactt tattggcaaa accaatctct ttaaagcgga
     1261 tattcatcgg gtagcgggct gcatagcctg tgatcaactg ctccagggcg accaccagac
     1321 cgaggtaatc caggctggca gggcgcagat ccatcgccag gcggtgcagt tcttccgata
     1381 cgctgtcggt cagctgcttc agctccgcaa tccgcagcaa ttggctttct ggtttttcag
     1441 catcctgctc gagcaggctc agtccaaact taagcgcggt aagtgactga cccgcttcat
     1501 catgcagctc gcgagcgata tagcgccgct cactctcctg gacttccacc agccgccggg
     1561 agagagactg cagccgctcg tgcccggagc gaacctgttc gaacagccag gcattctgga
     1621 tggatacggc agcctgggcg atcaccactt ccgcaagcca gacatgctcc tgattgattt
     1681 tgtagggttt cgaatttccc atcgccaaca cgcccatggc ttccccattc accaggatcg
     1741 ggattgccag ggagctgcga attggtaact ggctgaccag ggttgtccaa tcgggataca
     1801 agcgtgtatc gggaatgagc aagcttttat gggaggagag tacttcatgc agatagggtt
     1861 ggtcccagct gtcaaatgta tccttaagct ccatcttcgg atttatctgc aggtcgtaac
     1921 cgcggatcgc cagcagggtg aggctggact cattctcaca tacgccaata aaggcttgat
     1981 ccaccggtac caggtggcag acatactcta acagggtatt catcaccttt tcaaaattaa
     2041 gggtctccga aagcgcccgg ctgacttcat tcaaagtctc ggcgagctgg cgggcctgta
     2101 attcggcagc gtacaggcgg gcattctcca tcgccagcgc agcgggggca gcaaaaacca
     2161 tcaggcgccg ggctgtttca ggattaaaga cgccaggctg ttcgctggtc agattgatga
     2221 ccccggtcag gcggctgcca acaatcaagg gaactgccag gagggtgcgc ggcaaaggca
     2281 gcccgggaac cctgtaaaga tcgggagtct ctactgtatc ttcaaccagt agcggctggt
     2341 gggtggtacg catcacttca agcaagggga aatctgccag ctcggacgag cctgaaacag
     2401 catcagtgag caccttatcc tcagacaggt catgttggag gacgattctg aagattccat
     2461 tatcgatcaa caatatatta gcccgctgat attcgattgc ttcgtggatc tggtccagga
     2521 tggttttgag cacctcttca agctccaggc tggcatttac ggcgagcatg gtttggacca
     2581 ggctttccgc cagcttgcgc tgcttcgctt cggcgctgct aagggcgatt aattcttgat
     2641 tctggcgctt caataacagc tcagcccgct tgcgctcggt gacatctacc agactgagca
     2701 caacgccctg cacggagcca tccagctcaa acaccggctg tagacgccag ttccagtaac
     2761 ttataccccg ctcgggattg gcagcatatt cgaagggttt ttccaataca gagtaaggct
     2821 cacccgtatc taccaccttt tgaaaaatcg cctggttctc aagattgggg aataaatcaa
     2881 aatggttctt gccttcatag tatgctaccg gatgaccgtc tgaggtggca taagcctcgt
     2941 tgactttgat gaagttaaag tcacgatcca tgtaggcgat cagaatatcg atactgttga
     3001 aggctttgcc gatcagctcg ttggaccgga tcaggtcctg ttcagcctgt ttacgcaggg
     3061 tgatatcttc attaaccaca aagactccat ggacctgacc atgatcatcg cgcaccggga
     3121 tggcggtatt gaaaatcagc ttatgggtgc catcaaaaca ttcaatctca agctcctcat
     3181 tccaggaggt ctccccattg tggaccgccc gggcaactgc ccagtcttcc ggctcgaccg
     3241 gcaaccctgt actcacccac caggctttat actcgccaaa ttggtcaatc ccgacgtatt
     3301 ttgcccccgc ccagatctgc tggccggcag gattgccata taagatgttc ccgttttcat
     3361 ccgtcatcca ggcacctacc ggaaggacat cgaaggcggt tttgagcctg gcctcacttt
     3421 ttaccagcgc ctgctcagcc cgcttgcgct cggaaatatc ctggttgatg gcgatcaccc
     3481 cctgtaaggt ttgaccttcg aaaaagggag tggcagagtt gaggatgatt ttgtgggtgc
     3541 catcaaaaca ctcgatctcg agctcctcat ccgggatgga ctctccagac cggatggaac
     3601 gcgccgccgc ccaatcggca tgctcgacca gttttccatt atccagccgc caggctttat
     3661 attctccata ttgatccaca ctcacatagc gggctcccga ccagatcttc agactggcct
     3721 ggttgccatg caggatattc ccctgcgcat cggtgatcca gacccccacc ggcaggtttt
     3781 ccagcacgcg actcaacagc tcttcgctgc ggatgcgctc gctgacctgg ttgaataatg
     3841 ggcggaagtg gaaaaagtaa agcaaaggta tga
//
LOCUS       DATMXK010000096         4039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_167778, whole genome shotgun sequence.
ACCESSION   DATMXK010000096 DATMXK010000000
VERSION     DATMXK010000096.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167778"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..181
                     /locus_tag="VN363_01755"
     CDS             <1..181
                     /locus_tag="VN363_01755"
                     /inference="COORDINATES: protein motif:HMM:NF012698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWQ83257.1"
                     /translation="VMLVQGYETSEWEAPEVLRPLISRLRRKLSEFPHGEHWISNVRG
                     TGYVFDWQEDMAASN"
     gene            complement(195..1145)
                     /locus_tag="VN363_01760"
     CDS             complement(195..1145)
                     /locus_tag="VN363_01760"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWQ83258.1"
                     /translation="MQTRVKGISAALMSAFFLGMAPVFGKQAIQAGMAPFAVVAIRTS
                     AAALLLLLVVVLFFRRYLYIYPAGMLICFLAGGINGIGSLFYYSALGRLDASLGQLLY
                     ALYPLFLVLWLTMTGKRISYITILRLGLAIPAVILLSQTDTQTIDWIGIAQMLIAAAL
                     YALHLPVNQHVLYDMPAPTVTLYTLIAMSLVVVPAYFINGISSLPALGAISMPVIGLT
                     LVTFFSRLTLFQGVKNIGGMQTAILGLGELLVTVLFAHLWLDERLTVAQWVGTGLLLV
                     SLGLVIIDSANGSVKKSSWPNRLRPAATQELTFPENKGKL"
     gene            complement(1189..2907)
                     /locus_tag="VN363_01765"
     CDS             complement(1189..2907)
                     /locus_tag="VN363_01765"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWQ83259.1"
                     /translation="MIGNRIIECLDCGHKLSFEPGFTACPNCMSQWQEIHYAYPEIGA
                     ALPGLLARRPFDLWRYRELLPVLDYQPRMSLGEGGTPLIHAGNLGMMLGIPQLYIKDE
                     RQGPTNSFKDRQAAVSVAALKEAGITEAVVCSTGNVAIAFSAYAARAGIKLWAFLTSL
                     VPGEKMREVAIYGTQVIKVTGTYDEAKKIAAEFASQRGLYIDRGARSIPSVEAMKTIA
                     FEIAEQLTWLAQQDNPGLGDRLARANSQQVEWITPDWYFQSVSGGLGPLGVQKGFHEL
                     RLAGLTSSIPAIAGIQTAGCAPMAQAWNQGLEIANPVHSPRTHIATLSTGDPGRTYTL
                     LRQRMLAESNSHPGGCFDQVTDEEAFRAMHILAKLEGISVEPASAVAFAGLIKLVREG
                     LIKSNEVVVVNCTGHTVPVESTVLGDNWSRSVVAPSQAPEETPQEGLLAALSQVTSDR
                     YSKILIVDDTEDARRLIRRILQSQGNYTIVEASDGYQALELVHSELPDLIILDLMMPE
                     IDGFAVLDQLKSNPQTAPIPIIVVTAKELTQAEKKRLNGHIHKLMQKGEFMNDELLDE
                     VRALVK"
     gene            complement(2953..>4039)
                     /locus_tag="VN363_01770"
     CDS             complement(2953..>4039)
                     /locus_tag="VN363_01770"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ83260.1"
                     /translation="GSIAIKAFKQTGPDGKPEIMLQITDTGPGIALEHQAKLFQPFSQ
                     VDASLTRKTGGTGLGLSICRHLVELHGGRIGLESEVGIGSTFFFTIPVQLPATGSLTA
                     PDARLVLVIDDERPIVQLYERYLNRHGYRVIGLSEPQDALRRAREVMPYAILLDVMMP
                     GRDGWHVLEDLKSDPDTCSIPVIMCTILESQAKGQSLGADGYLTKPILEEELVHTLSN
                     LEVKAYKYNILGITDNDREFERFQAVFKDEPDYQLGLAFGGHQAISSMQFRRPDAILL
                     AIEPLKTKSTHPLSGLSVISLLETIRNDPLLQDIPVILFAELPLDARHGARIQELATA
                     VIPKNDWAKETVMEKIQQLLNQKNPAS"
BASE COUNT          945 a         1083 c         1062 g          949 t
ORIGIN      
        1 ggtcatgctg gtccagggat atgaaacctc ggaatgggag gccccagaag tactccgccc
       61 attaatcagc cggctgcggc ggaagctttc tgaattcccc catggggaac attggatttc
      121 caatgtacgc gggacaggct atgtttttga ctggcaggag gatatggccg ccagtaattg
      181 aggatgaaaa tgggttaaag ctttccctta ttttctggga atgttagctc ttgtgtagca
      241 gctggtctca acctgttggg ccagctgctt tttttaaccg aaccattggc actgtcgatg
      301 atcaccagcc cgaggctgac cagtaataac cctgttccaa cccactgcgc cacagttaag
      361 cgttcatcca gccagagatg ggcgaataat actgttacca gcagctctcc caaacccagg
      421 atagcggttt gcatgcctcc aatattcttt acaccctgga acagcgtcag gcgcgagaaa
      481 aatgtaacca gggttaaacc gatcacaggc atactgatcg ccccaagggc aggcagactg
      541 gatatcccat tgataaagta cgctggcaca accacaagac tcatggcgat caaggtatac
      601 aatgtcacgg tgggagcggg catatcatac aggacgtgct gattaactgg taaatgcaga
      661 gcatataatg cggctgcaat cagcatttgg gcgatgccta tccaatcgat agtttgagta
      721 tccgtctggc tcaacagaat cactgccggg atggccagac ccagccgtaa gatcgtgata
      781 taggagatcc gttttccggt catggtcagc catagcacca ggaacaaggg gtataaggca
      841 tataagagct ggcctaaact ggcatcaagc cgacccaggg cggagtaata aaaaagcgag
      901 ccgatcccat tgatcccgcc agctaaaaag caaattaaca tcccggcggg atagatatac
      961 aggtagcgtc gaaagaataa gactacgacg agcaggagaa gcagagcggc tgcagaggtg
     1021 cgaatcgcca caacagcaaa cggcgccatt ccagcctgga tggcttgttt gccaaatact
     1081 ggcgccattc ccagaaagaa cgccgacatc aatgctgctg agataccttt tacacgcgtc
     1141 tgcatttgat ttcctgttgg cagtgaattc tttactttct cagcatgatc atttcaccag
     1201 ggcgcgcact tcatcgagca gctcgtcgtt cataaactct cccttctgca taagcttgtg
     1261 aatatggccg ttgaggcgtt tcttttcggc ctgggtcagc tcctttgcgg ttacaacgat
     1321 aatcgggatg ggtgcagttt gtggattcga cttgagctgg tccagtacgg caaagccatc
     1381 aatttcgggc atcatcagat cgagaataat cagatcaggc agctctgaat gcaccagttc
     1441 cagcgcctga tagccatcgc tggcctcaac gattgtgtaa tttccctgcg actggagaat
     1501 tcgccggatt aagcgccggg cgtcttcggt atcatcgacg atcaggatct tagaatagcg
     1561 gtcgctggtc acctggctga gggctgccag gagaccttcc tggggagtct cttccggagc
     1621 ctggctgggt gcgaccacac tgcgtgacca gttatcaccc aggactgtgc tttcgacagg
     1681 tacagtatgt ccggtgcagt tgaccacgac gacttcattg gatttgatca agccctcccg
     1741 gaccagcttg atcaaaccag caaatgccac cgccgaggca ggctctacag aaataccttc
     1801 taattttgcc aggatgtgca tggcccggaa ggcttcttcg tcagtgacct gatcgaagca
     1861 tccccccggg tgtgaattgc tttctgccag catccgctgg cgtaataatg tataggtccg
     1921 tccggggtct ccggtggata gggtggcaat atgcgtccgc ggcgaatgca ccggattggc
     1981 tatctccagc ccctggttcc aggcctgggc cattggagcg cagccagctg tctggattcc
     2041 tgcaattgca ggtatggaac tagttaaacc ggccaggcgc agctcgtgga aacctttctg
     2101 gacacccagg gggcctaatc ccccgctgac tgactgaaaa taccagtccg gggtgatcca
     2161 ctctacctgt tgactgttag ctctcgcaag acggtcaccc agcccaggat tatcttgctg
     2221 agcaagccag gtcagttgtt cggcgatttc gaaggcgatc gttttcatcg cttcgacgga
     2281 ggggatagag cgggcaccgc ggtcgatata caatccccgc tggctggcaa attcagcagc
     2341 tatttttttg gcttcatcat aagtaccggt taccttgatc acctgtgtgc catagatcgc
     2401 aacttcgcgc atcttttcac caggtacaag gctggtaagg aatgcccaca gcttgatccc
     2461 ggcccgagct gcgtaagcgg agaaggcgat cgcaacattc ccggtagaac atacaaccgc
     2521 ttccgtgatt ccagcttcct tcaaagctgc cacactgacc gcagcctggc gatctttaaa
     2581 cgaattcgtc ggcccctggc gttcgtcctt gatgtacagc tgcgggatgc ccagcatcat
     2641 tccaaggttg ccagcatgga tcaacggtgt tcccccctct cccagtgaca ttcggggctg
     2701 gtaatccagg acgggcagca gctcgcggta gcgccacagg tcgaagggcc gccttgccag
     2761 caatcccgga agagcggcgc caatttctgg atacgcgtag tgaatttcct gccactggct
     2821 catgcaattt gggcaggcgg taaagcctgg ctcaaagctt aatttgtgcc cacaatccag
     2881 gcactcaata attcggttgc cgatcaatta tgcctccgca aaggaaaggt gtaggatgaa
     2941 ctgccagata gattaactgg ccggattttt ttgattcagc agttgttgga ttttttccat
     3001 cacggtttct tttgcccaat cgttcttcgg gatcactgct gttgcaagct cctggatgcg
     3061 ggcaccatgc cgggcatcga gcggcagctc tgcaaacaag atcacaggga tatcctgcag
     3121 aagcggatca ttccgaatag tctccaacag gctgataacg cttaatccag ataatgggtg
     3181 tgtggatttg gttttcaacg gttcaattgc caggaggatt gcgtcggggc ggcggaattg
     3241 catagaagaa atcgcctggt gcccgccaaa tgccagccct aactggtaat ctggctcatc
     3301 tttaaatacg gcttgaaaac gctcaaattc acggtcatta tctgtaatac ccagaatgtt
     3361 atatttataa gcctttactt caagattgct cagtgtatgc accagctctt cttccaggat
     3421 aggcttggtt aagtaaccat ccgcccccag gctctggcct ttcgcctggc tttcgaggat
     3481 ggtgcacata atgaccggga tcgagcaggt atccgggtcc gatttcaagt cctcgagtac
     3541 atgccagcca tcgcggcccg gcatcatcac atccagtaag atggcgtaag gcatgacctc
     3601 cctggcccgg cgcaatgcat cctgtggctc gctcagacca atcacgcggt aaccatgccg
     3661 gttcaggtat ctttcgtaca attggacgat tggccgctca tcatcgatga ccagaaccag
     3721 cctggcgtct ggggctgtaa gacttccagt tgcagggagc tgaacaggga ttgtaaagaa
     3781 gaatgtgctc ccgatgccga cttcactttc caatccaatc cgcccaccgt gcagttccac
     3841 cagatggcgg cagatggaga gacccagccc ggttccaccc gtcttccggg tcagcgaagc
     3901 atccacctgc gagaaaggct ggaaaagctt cgcctggtgc tccagagcga tccccggacc
     3961 ggtatctgta atctgcagca tgatttcagg ttttccatcg ggtccggttt gtttgaatgc
     4021 tttgatcgcg atgctgccc
//
LOCUS       DATMXK010000097         3048 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_76892, whole genome shotgun sequence.
ACCESSION   DATMXK010000097 DATMXK010000000
VERSION     DATMXK010000097.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3048)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3048)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3048
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76892"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /locus_tag="VN363_01775"
     CDS             <1..821
                     /locus_tag="VN363_01775"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF013296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016832 - aldehyde-lyase activity
                     [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWQ83261.1"
                     /translation="VDLSKETLAEQQALNTSLSAMFTALIREIEPAGVTISVGGEIGE
                     VGGQNSTEAELVAYMEGYQTALAKSAPGKPGISKISVQTGTSHGGVVLPDGSIASVSV
                     DFDTLLRLSRVARHEYGMGGAVQHGASTLPEDAFGKFVESEACEVHLATNFQNIFFNH
                     IPDDLKAEMYAYLDVKNAGDRKPELTAEQFYYKTRKNAIGPFKAKSWGLPADYRDAIG
                     SEWEKQFLKLFNLLGLVGTRKYVTQTILAVAVPPDVRDYLGEAVLEEDVSDLAD"
     gene            924..1436
                     /locus_tag="VN363_01780"
     CDS             924..1436
                     /locus_tag="VN363_01780"
                     /inference="COORDINATES: protein motif:HMM:NF012514.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NUDIX domain-containing protein"
                     /protein_id="HWQ83262.1"
                     /translation="MPASDQGLDQQRYTVVPRTLIFLRRDDKVLLLRGAPHKRLWANR
                     YNGIGGHVERGEDILSAARRELREEAGLEPAQLELCGVLSVDAGPDTGVIVFILTGEL
                     PQGEPLASSEGTAEWLAVDALGDLPLVEDLHILLPRSLAQAPGDPPFFARSYYDEQGK
                     LQVVINDTSR"
     gene            1502..1984
                     /locus_tag="VN363_01785"
     CDS             1502..1984
                     /locus_tag="VN363_01785"
                     /inference="COORDINATES: protein motif:HMM:NF024986.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pentapeptide repeat-containing protein"
                     /protein_id="HWQ83263.1"
                     /translation="MAELTRTELIKLIAANSGKGLTLRGVDLTGLNLSHLYLAEANLA
                     YANLTEASLTQADLTKANLEYAILDYADLSESILQFANLHRARLIGADLRAVDLRDSD
                     LREADLRGANLRTSDLTRANIWHADLREANLIYTNLDGAKGVSPEVEEWLKAGREPSE
                     "
     gene            complement(2075..>3048)
                     /locus_tag="VN363_01790"
     CDS             complement(2075..>3048)
                     /locus_tag="VN363_01790"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DMT family transporter"
                     /protein_id="HWQ83264.1"
                     /translation="AIISHPQNQEQPALPSVFRPQPPSATSSITPSSSPTAKTYTDWQ
                     AVGLVALGAVCYSSVIIFTRQSQGLSALSVSFFRAFFGFVFSSLLLLILRQPVRWSAL
                     KPYWKLLLLQSILMSGAAILYVYSVQHTTAANAALLVNSAPIYVALLAPLVLKERSPR
                     YTRLSLVLILAGVVLLTGVLDQNGGVISWPGLIAGFFAGANFGGVVLVSRAQHGQVPG
                     LIQTWIGTAFASLLLLPWGLSASGELVSAYLPSLVGLGVLSYGLPSFLYFVSLQRLKA
                     QVVSVVALLEPVAGALIGMLVYQEMFTLAALAGSLLVLVSIYLIAQE"
BASE COUNT          707 a          896 c          918 g          527 t
ORIGIN      
        1 tggtggacct ttccaaggag actctggctg aacagcaagc cctcaatacc agcctatcgg
       61 ccatgttcac cgctttgatc cgcgagatcg agccagcggg agtgactatc tcggtcggcg
      121 gcgagatcgg cgaagtgggc gggcaaaatt ctacggaagc cgagctggtc gcctacatgg
      181 aaggttacca gaccgccctg gcaaaatctg ccccgggtaa gcccggcatc agcaagatca
      241 gcgtccagac gggaacttcc cacggcggcg tggtcctgcc agatgggagc atcgccagcg
      301 tctcggtaga cttcgacacc ctgctgcgcc tcagccgcgt ggcgcgccat gagtatggca
      361 tgggtggcgc agtccagcac ggcgcatcca ctctgccaga agatgccttc ggaaaattcg
      421 tcgaatcgga agcctgtgaa gtccatctgg cgaccaattt tcagaatatc ttcttcaatc
      481 acattccaga cgacctgaaa gccgagatgt atgcctacct ggatgtgaag aatgccggcg
      541 atcgcaagcc agagttgacc gccgagcagt tctactacaa gacccgcaag aacgccatcg
      601 gccccttcaa ggccaaaagc tgggggctgc ccgcagacta ccgggatgcc attggcagcg
      661 aatgggaaaa acaattcctg aagctgttca acctgctggg attggtaggc acccgcaagt
      721 acgtcaccca gacgatcctt gcggtcgccg tcccacccga tgtccgggat tacctgggcg
      781 aagcagttct ggaagaggac gtttccgacc tggcggactg agccgggtta agcatctacc
      841 tgccatcccg agagatttac cgggcaatcc agatgggaat ctgagcggcc cggtaaatca
      901 atagaccgaa tggggagccc ggcatgcctg cctcagacca gggactggat caacaacgct
      961 ataccgtggt gccgcgcacc ctgatctttt tacggcgaga cgacaaagtc ctgctgctgc
     1021 gcggggctcc ccacaagcgc ctgtgggcga atcgctacaa tggaattggc gggcatgtgg
     1081 aacgcggcga ggatatcctc agcgccgcgc ggcgtgagct gcgcgaagaa gccggtctcg
     1141 agccagctca gctcgagttg tgcggggtgc tgagcgtgga tgcgggaccg gacaccgggg
     1201 taatcgtgtt catcctgacc ggtgagctgc cccagggtga gccgctcgcc tcctccgaag
     1261 gcacagccga atggctggcg gtggatgcgc tgggcgatct gccgttggtg gaagatctgc
     1321 acatcctgct gccgcgcagc ctggcgcaag ctcccggcga cccgccgttc ttcgctcgtt
     1381 cgtactatga tgagcagggt aagctgcagg ttgtgatcaa tgacacgagc cgctaagttg
     1441 ggtataatct ttattaagca atccagaacc tgaacccgca aacgcgcaag gagaaacccc
     1501 catggctgag ctgacccgca cggaactcat caaattgatt gcagccaact ctggaaaagg
     1561 gctcaccctg cgcggagtgg atctgacggg attgaatttg agccatctgt acctggcaga
     1621 agccaacctg gcgtacgcga acctgaccga ggcatctctg acccaggccg acctgaccaa
     1681 agccaacctg gaatacgcta tcctggatta cgccgatcta tctgagagca tcctgcagtt
     1741 tgccaacctg caccgcgccc ggctgattgg cgccgacctg cgggcagtgg atttgcgcga
     1801 ctcggatcta cgcgaggccg atctgcgcgg ggccaatctg cgcaccagcg acctgacccg
     1861 cgccaatatc tggcatgccg acctgcgcga agccaatctg atttacacga atctggatgg
     1921 cgcgaaaggt gtatcgcccg aagtcgaaga gtggctcaag gccgggcgtg agcccagcga
     1981 gtgagctgtg cgccctggat tacccgaatt gaattaccat ttgagtgaat aaccaatcgg
     2041 gcgacctgga ttaactggcg cctggattcc cccgttactc ctgagcaatc agataaatac
     2101 tcaccagcac cagcaggctg ccagccagcg ctgccagggt gaacatctct tgatacacca
     2161 gcatgccgat caaagccccc gccaccggct ctaacaatgc caccacgctg accacctggg
     2221 ctttcaggcg ctgaagggaa acaaagtaca ggaagctggg caatccatac gagaggacac
     2281 ccaatcccac cagcgagggc aggtaagcgc tcaccagctc accggaagcc gagagccccc
     2341 agggcaggag cagcaggctg gcaaaggctg tgccaatcca ggtctggatc aaacccggca
     2401 cctgcccgtg ctgagcacgg ctgaccagca ccacgccccc gaaattagcc ccggcaaaga
     2461 aaccggcgat cagaccgggc caggagatga ccccaccgtt ctggtccagc acaccggtaa
     2521 gcagcaccac cccggcgagg atcaaaacca ggctgagccg ggtatagcgc gggctgcgct
     2581 ccttaagcac cagtggggcg agcagcgcca cgtagatggg ggcagaattg accagcaggg
     2641 cggcgttggc ggcagtggta tgctgcacag aataaacgta caggatcgct gccccgctca
     2701 tgaggatgct ttgcaagagc agcagcttcc agtaaggttt cagagcgctc cagcggactg
     2761 gctggcgcag gatgagcaaa agcaagctgg aaaaaacaaa tccaaagaat gcccggaaga
     2821 atgacacact gagggcgctc aggccttgcg attggcgggt gaagatgatc accgagctgt
     2881 aacagactgc ccccagcgcg accaatccga ccgcctgcca atcggtatag gttttagcag
     2941 tgggactgga agacggggtg attgaactgg tggctgaggg aggttggggg cggaagacag
     3001 atggcaatgc aggttgttcc tggttctggg gatggctaat tatagcct
//
LOCUS       DATMXK010000098         2508 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_42978, whole genome shotgun sequence.
ACCESSION   DATMXK010000098 DATMXK010000000
VERSION     DATMXK010000098.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2508)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2508)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2508
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42978"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..890
                     /locus_tag="VN363_01795"
     CDS             <1..890
                     /locus_tag="VN363_01795"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HWQ83265.1"
                     /translation="VVISGTGESLVDILAARAKVGERVAISQELVNCEFVPIPERKDW
                     TNTYTATGVDKYILKGGVVQVDQDASQRQPMTAIAYNDDFVYFIVIDGREPGVSRGMS
                     NYEIAQFAKNTLQATDAATLDGGGSSTMVVNGTVVNNTTCNFEDCTNLQSPNGQLREA
                     EIISEARPAAGESIDMGVWSEDIQAIEAIVPNGLLMVVVEPPQRFSAFPVGSTPQVFT
                     NTEFRLGPGRNYDRVAGVPEIPEGGKVTVVENINNMNGILVNGGYWWKVSYAGQEGWA
                     PLDVSQVDLPIKTFLPFIQ"
     gene            1154..2068
                     /locus_tag="VN363_01800"
     CDS             1154..2068
                     /locus_tag="VN363_01800"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWQ83266.1"
                     /translation="MSTQANNPLASLSEALAEAVKRAGAWTVTVEARRRIPASGIAYA
                     GDLILTAEHVVERDEDITVRLPDGSSQAARVAGRDAGRDLALLRLESAALAPAEIASE
                     AAQVGQLVLGLGRPSREVGIEASLGIISAIGGPARTGRGGLLESYLRTDTTPYPGFSG
                     GPLVNTAGKLVGLNTSGLSRGSALTIPASLAWEVAATLAEHGHIRRGYLGVRSQPVEI
                     TPAQQTVLGRTQASGLLLVWIEEGGPAAQGGLLVGDILAGLGEETVSDHDSLLAGLAG
                     SRVGQAVDVRILRGGQPLTLQVVVGERS"
     gene            2076..>2508
                     /locus_tag="VN363_01805"
     CDS             2076..>2508
                     /locus_tag="VN363_01805"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83267.1"
                     /translation="MSRIGLVAPVLAVRVGLNALLEAAGFEVSWQAASLAEIVPHLEE
                     CELVLLSTSSISRAELSQVFGEVEGRLGLLLLVDRVQEIPALTELGLRAWGVLPLEAS
                     ETELAAALQALEAGLIVSTPAFSGRMASSEPGAGGNPPEMES"
BASE COUNT          533 a          637 c          882 g          456 t
ORIGIN      
        1 aggtggtgat ttcagggaca ggagaaagct tagtagacat tttagcagcc cgagctaagg
       61 ttggggagcg ggtggcaatc tcccaagaac tcgtgaactg cgaatttgtt ccaattccag
      121 agcgcaagga ttggacgaac acttacaccg ccaccggggt agacaaatat atcctcaaag
      181 gtggagtggt tcaggtcgat caggatgcat ctcagcgcca gccgatgacg gcgatcgctt
      241 ataacgatga ttttgtgtat ttcattgtaa tcgatgggcg cgaacccggg gtgagccggg
      301 gaatgagcaa ctacgagatc gcccagtttg ctaaaaatac cctgcaggcc acggacgcgg
      361 cgaccctgga cgggggcggg tcttcaacca tggtcgtaaa tgggacggtg gtgaacaata
      421 ctacctgcaa tttcgaggac tgtaccaatc ttcagtcgcc aaatggccag ctgcgcgagg
      481 ctgaaataat cagcgaagcg agacctgcgg cgggtgaatc catcgatatg ggggtgtgga
      541 gtgaagatat tcaggcgata gaggcgatcg tacccaacgg gctgctgatg gtggtggtcg
      601 agccgccgca gcgcttcagc gctttcccgg ttggctcgac gccgcaggtg ttcaccaata
      661 cggagttccg gctggggccg gggagaaatt atgatcgcgt ggcaggcgtg ccggagatcc
      721 ccgagggtgg gaaggtgacg gtggttgaaa atatcaataa catgaacggg atactggtga
      781 atggtgggta ctggtggaag gtaagttatg cggggcagga gggctgggcg ccgctggatg
      841 tgagccaggt ggacctcccc atcaagacct tcctgccgtt cattcaatag ggcgatccag
      901 ctaacctaaa gaccaggcta acctaaagac caggctaacc taaagatcag gctaacctaa
      961 agaccaggtt gacctaaaga ccaggctaac ctaaagatca ggttgaccta aagaccaggt
     1021 tgacctaaag accaggttga cctgcccgtt tgacggatag cggagcacac cctttgcggg
     1081 tatgatcttt ttaagggatc cacaatccct ttaatggtaa ctgggatcta taaacgcaaa
     1141 ggagaaataa ttcgtgagta cacaggcaaa taatccattg gcttccttat cggaggcgct
     1201 ggccgaggcg gtcaaacggg cgggtgcgtg gaccgttacc gtcgaggcgc gccggcggat
     1261 cccggcgagc gggatcgcct atgccggcga cctgatttta accgccgagc atgtggtgga
     1321 gcgcgacgaa gatatcacgg tgcgcctacc ggatgggagc tcacaggcgg cgcgggtggc
     1381 cgggcgggat gccggccgcg acctggcgct gcttcgttta gaaagtgcag ccctggctcc
     1441 ggctgagatt gccagtgaag ccgcccaggt gggccagctg gtgctggggt taggacggcc
     1501 ctctcgtgag gtgggaatcg aggccagcct gggcatcatc agcgccattg gcgggccggc
     1561 gcgcaccggg cgaggcggcc tgctggagag ctatctgcgc acggatacga ccccctatcc
     1621 cgggttctcg ggcggacccc tggtgaatac agcagggaag ctggtagggc tgaatacttc
     1681 ggggttgagc cgcggctcgg ccctgaccat cccggccagc ctggcgtggg aggtggcagc
     1741 tacgctggca gagcacgggc atatccggcg cggttacctg ggcgttcgca gccagccagt
     1801 ggagatcacc ccggcccagc aaacggtgct gggaaggacg caggcaagtg ggctgctgct
     1861 ggtctggatt gaggaaggtg gaccggcagc ccagggcggc ttgctggtgg gcgatatcct
     1921 ggcgggattg ggcgaggaaa ccgtgagcga ccatgacagc ctgctggccg gactggcagg
     1981 cagccgggta ggccaggcgg tggatgtgcg gatcctgcgc ggtggacagc cgctgacgct
     2041 ccaggtggtg gtgggggaaa ggtcatgagg aagatgtgag ccggatcggg ctggttgcgc
     2101 cggtcctggc agtgcgggtt ggcctgaacg ccttgctgga agcggcgggc tttgaagtga
     2161 gctggcaggc cgccagcctg gcagaaatcg tgccccatct ggaagagtgc gagctggtcc
     2221 ttttaagtac cagcagcatt agcagggcgg agctcagcca ggtctttgga gaagtcgaag
     2281 gacgcctggg gctgctgctg ctggtggacc gggtgcaaga aatcccggcg ctgacggagc
     2341 tgggtctgcg cgcctggggt gtgctgccgt tggaagcgag cgagacagag ctggcggcag
     2401 ccctccaggc gctggaagcg ggcttaattg tcagcacacc ggcattctca ggacgcatgg
     2461 cgagcagcga acccggtgct ggcggaaacc cgccggaaat ggaaagcc
//
LOCUS       DATMXK010000099         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_121651, whole genome shotgun sequence.
ACCESSION   DATMXK010000099 DATMXK010000000
VERSION     DATMXK010000099.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121651"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..300
                     /locus_tag="VN363_01810"
     CDS             <1..300
                     /locus_tag="VN363_01810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560097.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ83268.1"
                     /translation="GQITGVEGYAGNIATGLLAGCNAARLWAGKPPLALPPETMLGAL
                     CQYITHASAQDFQPMKANYGIMPELEDSGVRGRRPRAAAFSQRALAVLDEFLKSI"
     gene            311..1318
                     /locus_tag="VN363_01815"
     CDS             311..1318
                     /locus_tag="VN363_01815"
                     /inference="COORDINATES: protein motif:HMM:NF016289.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M28 family peptidase"
                     /protein_id="HWQ83269.1"
                     /translation="MKPRVNIALLMFSAALGISLLAGCANEPRSNPNTGLSPDASQPP
                     AFTQTPEATEPPIMDFSGERALQDVVTQVEFGPRTPGSPAHTQTVEFIKTSLEANGWS
                     VEIQESIYQGQTVRNVVARTGSGSPLLLFGAHYDTRIYADHDPNPANHTQPVPGANDG
                     ASGVGVLLELGRVLSARMARLEGKPGQVWLVFFDAEDNGRIPGWDWIMGSRAFVDQLT
                     VRPDATVIVDMIGDADLNAYLEKNSTPELRDEIWGIAAGLGYGDQIINELKYGMTDDH
                     TPFLNAGLPAVDMIDFDYPYWHTVDDTVDKVAPASLEVVGNTLVAWIELRLGLPGEST
                     P"
     gene            1358..2644
                     /locus_tag="VN363_01820"
     CDS             1358..2644
                     /locus_tag="VN363_01820"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWQ83270.1"
                     /translation="MQILENLEMIRPAWSQQVAAQMARGVDVRLSFAEQLDHFYAGLI
                     QAIATGDPTRVEPVLNDWILVRSQSELKADKASLAPLVDQMLLSLSGVAGQALTAEDA
                     LALVGAALPVFSHVYTYTNQEETRLNFENYSRELEAVKLSLEELDKSKSDFISIASHE
                     LRTPLTLIEGYSAMLLENLSTSARDEQTAVLLHGIEKGTRRMHEIIEDMIDVSMIDNH
                     LLELNFQPVWMDRLVNQVVHGLSSSISDRQQNLEIRSFEGFNGRMFADNERLYQALYN
                     LVANAIKFTPDHGKVTIGGRCLPGFMELTIQDTGIGIDQEDQARVFDKFTGIRDASLH
                     SSSKTGFKGGGPGLGLAIAKGIIDAHGGTIWVESEGYDEQAFPGTTFHVLIPNRDRPP
                     EERISELFERTGGGKGLLNPAEKPTPYSIESGPASS"
     gene            2672..4048
                     /gene="hflX"
                     /locus_tag="VN363_01825"
     CDS             2672..4048
                     /gene="hflX"
                     /locus_tag="VN363_01825"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560101.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase HflX"
                     /protein_id="HWQ83271.1"
                     /translation="MAKKTPESTRPPREKAFMVGVDIRSAENMLPLDASLAELALLAD
                     TAGLEVVGQATQKLDHPHPQTFIGPGKVEEVRALVEELQAEVLLFDEELSPRHLRELE
                     EAFKGKINILDRTALILDIFAQHASTREGALQVELAQYEYRLPRLTRAWTHLARQAGG
                     GGGRSGSVGGVGLRGPGETQLEVDRRDIRRRIDRLKADLEKVRVHRQQYRSRRKRSRT
                     PVVALVGYTNAGKSTLLNKLAQSDVYVADQLFATLDPTTRRVELPGGNLALFTDTVGF
                     IQKLPTMLVAAFRATLEEIAEADLLLHVVDINHPQAQAQADAVFQTLSEIEADHIPVL
                     TVLNKMDQMSDPVQAAATVEEYPNTVAISARTGFGIEKLLAKIGEVLFETYTPILVFL
                     PFTEGGLISGFHELGQVEQAEDVRGGVRIKGRLPGRLLASYKAFEVLDTVDGAGLPAE
                     EMDDDELV"
     gene            4032..4244
                     /locus_tag="VN363_01830"
     CDS             4032..4244
                     /locus_tag="VN363_01830"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83272.1"
                     /translation="MTNWFNRFLDRMSEFLANRKGLLPMLGLALITANLILEFVFPGS
                     WLVTTDLLLQMGLILAISGFLLAWAL"
     gene            4261..>4754
                     /locus_tag="VN363_01835"
     CDS             4261..>4754
                     /locus_tag="VN363_01835"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWQ83273.1"
                     /translation="MITGSTLTHLTCPECNKEVSADRLQRYCADCASPWLAVYELAAL
                     RTELDRDRVSHRPRGMWRWAELLPVHSTANQVSLGEGDTPLLQLDRLGACLGLEKLFL
                     KDEGANPTGSFKARGMAAAISMAKELGVEKVIVPTAGNAGGAMAAYAARAGLQACVIM
                     PQDTP"
BASE COUNT          999 a         1330 c         1474 g          951 t
ORIGIN      
        1 gggcagatca ccggggtgga agggtatgcc gggaacatcg ccacaggcct gttggccggc
       61 tgcaatgccg cccgcctgtg ggcaggcaag ccgcccctgg ctttgccgcc cgaaaccatg
      121 ctgggggcat tgtgccaata catcacccac gccagcgccc aggatttcca gcccatgaaa
      181 gccaactatg ggatcatgcc tgagctggag gacagcggcg tgcgcggcag acgcccgcga
      241 gcggccgcat tcagtcagcg ggcactggcc gttttggatg aatttctgaa atcaatttga
      301 ggacctcatt atgaaacccc gggtcaatat tgctttgctc atgttcagcg ctgcactagg
      361 catttccctg ctcgcaggct gtgccaatga accgcgttcc aatcccaaca ctggattatc
      421 gccagatgcc agccaaccac ctgcgtttac ccaaacacct gaggctaccg agcctccgat
      481 catggatttc tctggggaaa gggccttgca ggatgtggtt acccaggtag agtttgggcc
      541 tcgaacgccg ggcagccctg cccacaccca gaccgtcgaa tttattaaaa ccagcctgga
      601 agcgaatggc tggagcgtcg agatccagga gagcatctat caagggcaga ctgtccgcaa
      661 cgtcgttgct cgcaccggca gcggctcgcc gctgctcctg tttggggcgc actatgacac
      721 gcgcatctat gcggaccatg atcccaaccc ggccaatcac acccaaccag ttcccggcgc
      781 caacgacggc gcttcgggtg taggcgtgct gctggagctg gggagggtgc tgtctgcccg
      841 catggcgcgt ctggaaggca aaccgggtca ggtgtggctg gtgttctttg acgccgagga
      901 caacgggcgc atcccgggct gggattggat catgggctcg cgagcgtttg tcgaccagct
      961 caccgtcagg ccggatgcga cagtaatcgt ggatatgatc ggggacgcag acctgaatgc
     1021 ctatctcgag aagaattcaa cccctgagct gcgcgatgag atctggggga tcgcggccgg
     1081 gctcgggtat ggtgatcaga tcattaatga gctgaaatac ggcatgaccg acgaccacac
     1141 gccgttcttg aatgccgggc tcccggcagt ggatatgatc gactttgatt atccttactg
     1201 gcacaccgtc gatgatacgg tcgataaagt cgctccagcg agtctggagg ttgtagggaa
     1261 tacattagtc gcctggatcg agctgcggct gggattgcca ggagaatcca caccctgacc
     1321 gttggcaaat agcaggggag aggataaact aagagccatg caaatactgg agaacctgga
     1381 gatgatccgc ccggcgtgga gccagcaggt ggctgcacag atggcgcgcg gcgtcgatgt
     1441 tcgcctgagt tttgccgaac agttggacca tttctatgcg ggtctgatcc aggcgattgc
     1501 gacaggcgat cccacccggg tggagccggt gctcaacgat tggatactgg tgcgctctca
     1561 atctgagctg aaggcggaca aggccagcct ggcaccgctc gttgaccaga tgctgctgag
     1621 tctgtccggc gtggcaggcc aggctttaac ggctgaggat gcgctggccc tggtgggtgc
     1681 agcgctgccg gttttcagcc atgtttatac ctataccaac caggaagaga cccggctgaa
     1741 tttcgagaat tattcccgtg agctggaggc ggttaaactt tccctggaag agctggataa
     1801 aagcaaatcg gatttcatct cgattgcctc ccacgagctg cgcacaccac ttaccctgat
     1861 cgagggctac agtgcgatgc tgctggagaa tttatccacc tcggcgaggg acgaacaaac
     1921 cgcggtcctg cttcatggaa tcgagaaggg gacgcgccgg atgcacgaaa ttatcgaaga
     1981 tatgatcgat gtctcgatga ttgataatca tctgctggag cttaattttc aaccggtctg
     2041 gatggaccgg ctcgtgaacc aggtcgtcca cgggctttcg agcagcatct cagaccgcca
     2101 gcagaacctg gagatccgca gttttgaggg attcaacggc cggatgttcg cggataacga
     2161 gcggctttat caggcgttgt acaatctggt ggccaatgcg atcaaattta ccccagatca
     2221 tggcaaggtc actatcggag ggcgctgcct gcccgggttt atggaattga cgatccagga
     2281 taccgggatc gggatcgacc aggaagacca ggcgcgggtc ttcgataaat ttaccggaat
     2341 ccgggatgca tcgctgcact cgagcagcaa aaccgggttc aagggcggag ggccaggcct
     2401 gggtctggca atcgccaaag gcatcattga tgcccacggt gggacaatct gggttgaatc
     2461 ggagggctat gacgagcaag ccttcccagg cacaaccttc catgtgttga ttccaaaccg
     2521 cgaccggcct ccagaggagc gcatcagcga gctgtttgaa aggaccggcg gtgggaaagg
     2581 tctgctgaat ccggctgaga aaccaacccc ctacagtatt gaatctggcc cagccagttc
     2641 ttagccgttt gagcgataga ggatttatta tatggcaaag aaaacccctg aatccacccg
     2701 tccacctcgt gaaaaggcat tcatggtggg ggtagatatt cgcagtgctg agaatatgct
     2761 gccgctggat gcctccctgg ccgagctggc actgctggcg gacacagccg gcctggaagt
     2821 tgtcgggcag gcaacccaga aattggacca cccccacccg cagacattta tcggtccagg
     2881 taaggtagag gaggtgcgcg ccctggtcga agagctgcag gcggaagtgc tcctgttcga
     2941 cgaagagctt tcgccgcgcc acctgcgcga gctggaggaa gctttcaaag gcaaaatcaa
     3001 tatcctggac cggacggcat tgatcctgga tatttttgcc cagcacgcca gcacccgcga
     3061 aggcgcactg caggtggagc tggctcaata tgagtaccgc ttgccccgcc tgacccgggc
     3121 ctggacccac ctggcccgcc aggccggggg cggtggcggg cgctcgggca gcgtgggcgg
     3181 cgtcggtttg cgcggcccag gtgagaccca gctggaagtg gaccggcggg atattcgccg
     3241 gcgcatcgat cgtctgaagg cagacctgga aaaagtgcgc gtccaccgcc agcagtaccg
     3301 ctcgcggcgc aaacgctcgc gtactccggt ggtggcgctc gtgggctata ccaatgccgg
     3361 taaatccacc ctgctcaata aactcgctca gtcagatgtt tacgttgccg accagctctt
     3421 tgccaccctg gatccgacga cccggcgggt agagctgccg ggcgggaatc tggccttgtt
     3481 tacggatacg gtcggcttca ttcagaaact gcccaccatg ctggtggctg cctttcgggc
     3541 gaccctggaa gagatcgctg aagcagatct gctcctgcac gtggtggata ttaaccaccc
     3601 ccaggcccag gcgcaggcgg atgccgtttt ccagaccttg tcagagatcg aagccgatca
     3661 tatcccggtc ttgaccgtgt tgaataaaat ggaccagatg agcgatccgg tgcaggctgc
     3721 cgccacggtg gaagaatatc cgaacaccgt ggcaatctcc gcccggacag gctttgggat
     3781 cgaaaaactg ctggcgaaga tcggcgaggt actcttcgag acctatacgc cgattctggt
     3841 ctttcttccg tttactgaag gcggcctgat atccggtttc cacgagctgg gccaggttga
     3901 gcaggcagag gatgtgcgcg gcggagtgcg cattaaaggc cgtctgcccg ggcggctgct
     3961 ggcaagctat aaagcatttg aggtgctgga tacagtcgat ggcgcggggc tgccggcgga
     4021 ggaaatggac gatgacgaac tggtttaatc gatttttgga ccggatgtca gaattcctgg
     4081 ccaaccgcaa aggcctgctg cccatgctgg gcctggccct gatcactgcc aacctgatcc
     4141 tggaatttgt gttcccaggg agctggctgg tgaccacgga cctgctgctg cagatgggac
     4201 tgattctcgc catctcgggt tttctactgg cctgggcgct ttaattaacg gatctacgct
     4261 ttgattaccg gatcaactct cacccattta acctgcccgg aatgtaataa ggaggtctct
     4321 gcagaccggc tgcagcgtta ctgcgcagac tgcgcatccc cgtggctggc ggtttatgaa
     4381 ctggcagcgc tccgcacgga gctggatcgg gaccgggttt ctcaccgccc gcgagggatg
     4441 tggcgctggg cagagctgct cccggtgcac tcgaccgcga accaggtcag cctgggcgaa
     4501 ggcgatacac ccctgctgca attggaccgg ctgggtgctt gcctgggcct tgaaaagcta
     4561 tttctgaagg atgaaggcgc caatccaacc ggctctttta aagcccgcgg gatggcagct
     4621 gcgatttcaa tggcgaaaga gctgggcgtt gagaaagtga tcgtcccgac ggcggggaat
     4681 gccgggggag ccatggcagc ctatgccgcc cgcgccggac tgcaggcgtg tgtgatcatg
     4741 ccgcaggata cccc
//
LOCUS       DATMXK010000100         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_10384, whole genome shotgun sequence.
ACCESSION   DATMXK010000100 DATMXK010000000
VERSION     DATMXK010000100.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10384"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..481
                     /locus_tag="VN363_01840"
     CDS             <1..481
                     /locus_tag="VN363_01840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="N-acetyltransferase"
                     /protein_id="HWQ83274.1"
                     /translation="QLPANLQLIPWNAELNEAARQTVNEAFADHWGSIPTNPEHWKMW
                     VTEHEYFHPELSFMVTDGEDLAGVSVNKIRSAENKVIGIKEGWIQTLGVRRAWRRQGI
                     ASALIGASMQAFKAAGLEYAGLTVDTDNFTGALRIYERLGFKDIARIISFAKTINPE"
     gene            486..1844
                     /locus_tag="VN363_01845"
     CDS             486..1844
                     /locus_tag="VN363_01845"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ83275.1"
                     /translation="MEQTVIHSQPVLRDLGEGLILRRSTPADADALADFNSIIHNESD
                     TQDRDLRIGAWTRDLLTFPHPTFDPGDFTIVEDSQTGKIVSSLNLINQTWTYAGIPFG
                     VGRPELVGTRLEYRKRGLVRAQFEEVHRWSAARDHRVQAITGIPHYYRQFGYEMTVNL
                     GGGRMGYRPQIVELGKDETDPYRVRPARLEDIPFLQSLYTQFCQRSLLSNVMDEAQWR
                     YQLSGRSEANVNHSETCIVENNENHAVAFFTHPSMLWGTILAATEFEILPGQPYLPVT
                     NCVIRYLRTTGEAYARQQGGTATWESFGFWLGSQHPVYDVLYERLPRERKPYAWYLRV
                     ADIPGFLHLIQPVLQQRLDKSPMAGYQGELRISFYRSGLRLAFEAGRLNAIEPWQPTP
                     VGHTGEAGFPNLTFLQLMFGYHSLEELADFLPDVYIHKPEAWPLLNSLFPKQASNIWP
                     VA"
     gene            1873..2481
                     /locus_tag="VN363_01850"
     CDS             1873..2481
                     /locus_tag="VN363_01850"
                     /inference="COORDINATES: protein motif:HMM:NF027788.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWQ83276.1"
                     /translation="MGTIFEQLQNLISVDIESAGSIPSRFALLSIGACTLTHPQETFY
                     TELQPDQDQIDPMALQVHGLDLQQLRQKGLPPADAMRRFADWLKNTLPPGEKPIMLAF
                     NAPFDWMFVNDYFIRYLGDNPFGHNAIDIKAVYLGATGKSWQETSGVYLHTRYHDGSK
                     LTHNALDDAIAQAEIFEGILREIQGNLQDINRANFPESNLSQ"
     gene            2496..>2773
                     /locus_tag="VN363_01855"
     CDS             2496..>2773
                     /locus_tag="VN363_01855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83277.1"
                     /translation="MAADEVNPTEEMNRILESARRLGVELNEQEATQWLVAISAAGSE
                     SDITYDEADGIFGHRITLLDFSPQRLAYFRKIGKLVEFSDEPGVVETAL"
BASE COUNT          628 a          801 c          745 g          599 t
ORIGIN      
        1 ccagctgccg gcaaacttgc agctaatccc ctggaatgct gagcttaatg aagctgcccg
       61 ccagacagtc aatgaagcct tcgcagatca ctgggggagt atcccaacca atccagagca
      121 ctggaagatg tgggtgaccg agcatgaata ttttcaccct gagctgtctt tcatggtgac
      181 ggatggagaa gacctggccg gtgtttcagt caataagatc cgctcggccg aaaataaggt
      241 gatcgggata aaagaaggct ggattcagac cctgggtgtg cggcgtgcct ggcgcaggca
      301 gggaattgca tccgccttga ttggtgccag catgcaggca ttcaaagctg ccggcctgga
      361 atacgctggc ttaacggtcg ataccgataa cttcaccggc gccctgcgaa tttacgagcg
      421 cctgggtttt aaggatatcg cccggattat ctccttcgcg aaaacgatca acccggagta
      481 gcctcatgga acaaacagta attcactctc aacctgtcct gcgtgatctg ggcgaaggcc
      541 tgatcctgcg ccgctcgaca cctgccgatg ctgacgccct ggcagatttc aattccatca
      601 tccacaatga atcagatacc caggaccgcg atctccggat aggcgcctgg acgcgcgatc
      661 tgctgacctt cccgcacccc acttttgacc ccggcgattt cacgattgtt gaagatagcc
      721 agacgggcaa gattgtctcg tctctcaacc tgatcaacca gacctggacc tatgccggca
      781 ttccttttgg ggtaggccgc ccggagctgg tgggcacgcg gctcgaatat cgtaagcgcg
      841 gcctggtgcg cgcccagttt gaggaagttc accgctggag tgctgcccgc gaccaccggg
      901 tgcaggctat caccggtatc ccccactact accgccagtt cggttacgaa atgacagtca
      961 acctgggcgg cgggcggatg ggctaccgcc cccagattgt ggaattggga aaagatgaaa
     1021 ccgatcccta ccgggtgcgt cccgctcgcc tggaagatat tcctttcctc cagagcctgt
     1081 atacccaatt ctgccagcgc tctttactca gcaatgtgat ggatgaagcg caatggcgct
     1141 accagctttc cggccgctcg gaggccaacg tcaaccacag tgaaacttgc attgtggaga
     1201 acaatgaaaa tcatgcggta gcgtttttca cccatccgag catgttatgg ggcaccattc
     1261 tggcagccac cgagttcgag atcctgccag gccagcctta cctgccagtc actaattgcg
     1321 ttatccgcta cctgcgcact accggtgaag cctatgccag acagcagggg ggaaccgcca
     1381 cctgggagtc gtttggcttc tggctgggga gccagcatcc cgtctatgat gtgctttacg
     1441 agcgactgcc ccgtgagcgc aagccttatg cctggtactt aagggtagcc gatatccccg
     1501 gatttttaca ccttatccag ccggtcttgc aacaacgcct ggacaaatcg ccaatggcag
     1561 gctatcaggg tgagctcagg atcagctttt accgctccgg cctgcgcctg gcgtttgagg
     1621 ccggccgttt gaacgcgatc gagccctggc agccgactcc ggtggggcat accggagaag
     1681 ctggtttccc caacctcacc ttcttgcagt taatgttcgg gtaccactcg ctggaagagc
     1741 tggcagattt cctcccggat gtctatatcc ataaaccgga agcgtggcct ctgcttaaca
     1801 gcctgttccc gaaacaggcc agcaatatct ggccagtcgc ctgaagcttg cgtctcgcga
     1861 tacaatacag gcatgggaac tatcttcgaa cagctacaaa atctaatcag tgtcgatatc
     1921 gaatcggctg gctctatccc cagccgattc gcgttattat ccatcggcgc ctgcaccctc
     1981 acgcaccccc aagagacctt ttataccgag ctccagccgg atcaagacca gatcgaccca
     2041 atggcattgc aggtgcacgg gctggatctc cagcagctgc gccaaaaagg cctgccgccg
     2101 gcggacgcca tgcgtcgctt cgcagactgg ctaaaaaaca ccctcccccc cggagagaag
     2161 ccgattatgc tggcgtttaa tgcaccattc gactggatgt tcgtcaacga ttatttcatc
     2221 cgctacctgg gtgacaaccc gttcggtcac aatgccatcg atatcaaggc agtttacctg
     2281 ggcgcaaccg gaaaatcctg gcaagagaca tcgggggtgt atctgcatac ccgctaccac
     2341 gatggctcca agctgaccca caacgccctg gatgatgcaa tcgcccaggc agagatattc
     2401 gaaggtattt tgcgtgaaat ccagggtaac ctgcaagata ttaaccgggc taattttcca
     2461 gaatcgaatc tatcgcaata attcaggagg aaatcatggc tgctgatgaa gtcaatccca
     2521 ccgaggagat gaaccgcatc ctcgaatcgg cacggcgttt gggtgtagag ctcaacgagc
     2581 aggaggcgac ccagtggctg gtggcaattt ctgccgcggg gagtgaatca gatattacct
     2641 atgacgaggc ggatgggatc ttcgggcacc ggattacgct gctggatttt tctccacagc
     2701 gcctggcata ttttcgcaag attggaaagc tggttgaatt ttccgatgag cccggtgtgg
     2761 tggaaactgc cct
//
LOCUS       DATMXK010000101         3012 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_18242, whole genome shotgun sequence.
ACCESSION   DATMXK010000101 DATMXK010000000
VERSION     DATMXK010000101.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3012)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3012)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3012
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_18242"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2778
                     /locus_tag="VN363_01860"
     CDS             <1..2778
                     /locus_tag="VN363_01860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWQ83278.1"
                     /translation="SNDFTPLSAPPPEVSWQAGTLFEFRIRGEDHAGNREDWPATPDA
                     VTRIESHPPHTSFIDPPAYARTGGLLYYQAADVGGSQVANYDVQRFNNDTQTWEMLAS
                     ETTSTEIGVGGLPGTSWTVRVRARDTAGNEEPWPQAGDPGYHTVGVYTAAIYGTAHAA
                     TGNPVSGVEVAVADGTLLAQENSDPGGAYASFVIYDTVIQAAFSKPGYGGLPWSTYAG
                     FLDYKLDLYLPPADNLVQDSDLVLGLASPASPWAVGGTLPITPAASQEPCHTGSCAAL
                     GSTSLIFTPFETVFPAALQTVDLRTYAASDNSLHLIAHMGTDLYYRQRTAAGTWLPYE
                     TIHTAAANFILAAEVKTDASGVAHLIYLHNGQVFYIQRSLAGAWSSPRRISGEANANS
                     DWSISDLLQVDAQGGVHAMWQCNWDGLYSDVCYARRTPAGSWSAPEVVESVQGAHMQI
                     RFRLTSSGIVHAAWSRESSSPPAILYARRSVDGTWSSPVELGQFSDYAELRFTFELDA
                     SQRPMVAWVVYGGFQNFLIRQRWQQANGSWSALQERDLGIYYTYYPYAKLAYDSQARL
                     HMLFGNDFHYFILPRSGELERRGVIVPDLFEVNDFSLAPDGKIHLVYRLNNRVGAVYS
                     LLEGGEIRETLFFTDFFAPTDAPWMFVDASQTPHFLYHRDEILGGEQVYVWDHTTRAR
                     AASAETGELSQTVTIPGGMNQAGLSLLYLLKGGQAAGSTGLSAWIEAGDGTQEMWHTH
                     TGSIDWQHAWVDVSDYAGETVRVILRMDQAAGEVRMFGYLDEVSLGTGYGDAWLGVDA
                     QERKVRPDEPFAVEIPYGNQGGFALPGGVIEVSLPAGLELLDSSLACTGGPAVYSCAA
                     GTLAARAEGVLSLSLRSTPGAPAFQRLEIGLRLATTGSELELLNNQGILGVRLANELF
                     VPAIGNWWVGE"
BASE COUNT          549 a          928 c         1009 g          526 t
ORIGIN      
        1 tcgaatgatt tcacccccct cagcgccccg ccgccggagg tcagctggca ggccgggacg
       61 ctgtttgaat tccgcatccg cggcgaagac cacgccggca accgggaaga ctggccggcg
      121 acgcccgacg cggtcacacg catcgaaagc cacccgccgc ataccagctt cattgatccg
      181 ccggcgtatg cccgcaccgg aggcctgctg tactaccagg ctgcggatgt gggcggttcg
      241 caggtggcga actacgatgt ccaacgcttt aataatgaca cccagacctg ggaaatgctg
      301 gcctcggaga ccaccagcac cgagatcggg gtcggcgggc tgcccggaac cagctggacg
      361 gtgcgggtgc gcgcccggga tacggccggg aatgaggaac cctggcccca ggccggtgat
      421 ccgggttatc acacggtagg ggtatatacc gcagccattt acggcaccgc ccatgccgcc
      481 accggcaacc ctgtctcagg ggtggaagtg gcagtggcgg atgggacgct gctggcccag
      541 gaaaacagcg acccgggcgg ggcatatgcc agctttgtca tttacgacac ggtcatccag
      601 gcggcgttct ccaaacccgg ctacggcggc ctgccctggt cgacctatgc gggcttcctg
      661 gactataaac tggacctgta cctgccgccg gcggacaacc tggtccagga cagcgacctg
      721 gtgctgggcc tggcatcgcc ggctagccca tgggcggtag gcgggacgct gcctatcacg
      781 ccggcggcca gccaggagcc ctgccatacc ggcagctgcg ccgccctggg cagcaccagc
      841 ctgatcttca cccccttcga gacggttttt ccggctgccc tgcagacagt cgacctgcgg
      901 acctatgccg cgagcgataa cagcctgcac ctgatcgccc atatgggcac tgacctgtat
      961 taccgccagc gcaccgccgc gggaacctgg ctgccgtacg agacgatcca tacggcggcg
     1021 gccaatttta tcctggcggc cgaagtcaag acggatgcca gcggggtggc gcacctgatt
     1081 tatctgcata acgggcaggt tttttacatc cagcgcagcc tggcgggcgc ctggtccagc
     1141 ccccgccgga tttccgggga ggcgaacgcc aacagcgact ggtcgatctc cgatctgctg
     1201 caggtcgatg cccagggcgg ggtgcacgcc atgtggcagt gcaactggga cgggctgtac
     1261 agtgatgtgt gttacgcccg gcgcaccccg gctggcagct ggagcgcccc ggaggtggtt
     1321 gaatccgtgc agggagcgca tatgcagatc cgcttccggc tgaccagcag tgggatcgtg
     1381 cacgccgcct ggagccggga aagttcatcg cccccagcga tcctttatgc gcggcgcagc
     1441 gtggatggga cctggagcag cccggtagaa ctgggccagt tcagcgacta cgctgagctg
     1501 cggttcacct ttgagctgga tgccagccag cggccgatgg tcgcctgggt tgtgtacggc
     1561 gggttccaga acttcctcat ccgccagcgc tggcagcagg ccaacggcag ctggtcggcg
     1621 ctgcaggaac gggatctggg gatctactac acttactacc cctatgcaaa actggcgtac
     1681 gacagccagg cgcgcctgca catgcttttc ggcaatgact ttcactactt catcctgccg
     1741 cgcagcggtg agcttgaacg gcggggggtg attgtaccgg acctgtttga ggtgaatgac
     1801 ttcagcctgg cgcctgatgg gaaaatccac ctggtgtacc gcctgaacaa ccgcgtggga
     1861 gccgtttaca gcctgctgga gggcggagaa attcgtgaaa cgctgttctt taccgacttc
     1921 tttgcgccca cggatgctcc ctggatgttc gtcgacgcct cccagacacc ccatttcctg
     1981 taccaccgcg atgaaattct cggcggggag caagtctacg tctgggacca caccacccgg
     2041 gcacgggctg ccagcgccga aaccggtgag ctctcccaga ccgtgaccat ccccggcggg
     2101 atgaaccagg ccgggttgag cctgctctac ctgctcaagg gcgggcaggc tgccggcagc
     2161 acggggcttt cggcgtggat cgaagcgggg gatggcacgc aagaaatgtg gcatacgcac
     2221 accggaagca ttgactggca gcatgcctgg gtcgatgtct cggattacgc cggggagacg
     2281 gtgcgcgtga ttttacgcat ggaccaggcg gctggcgagg tgcgtatgtt cggctacctg
     2341 gacgaagtga gcctgggcac ggggtatggc gatgcctggc tgggggtgga tgcacaggaa
     2401 cgcaaggtgc ggccggacga gcccttcgcg gtcgagatcc cttacggcaa ccagggcggg
     2461 tttgccctgc ccggaggcgt gatcgaagtg agcctgccgg ccgggctgga gctgctggac
     2521 agcagcctgg catgcaccgg tggaccggcg gtgtattcgt gcgccgcggg cacgctggca
     2581 gcccgggcgg aaggtgtgct cagcctgagc ctgcgcagca ccccgggagc gccggccttc
     2641 cagcgcctgg agatcggcct gcgcctggcg accaccggca gcgagctgga gctgctgaac
     2701 aaccagggaa tcctgggtgt cagactggcg aacgagctgt ttgtacctgc aatcgggaac
     2761 tggtgggtgg gagagtagcc ggggaggctg agataaagaa ttaaccaaga acccgagttt
     2821 tggctctcga aactcgggtt ctatttttca ggcgcgcatg caggaaagga tctgttcggc
     2881 ggtcaggtag atcatcaacg cgccagggct tccaaagccg ggcgatattc ttcgataaat
     2941 tcggccacct ggcgggcgaa gtcctcgtct atacccgctg cagccagttc ttcgggcggc
     3001 agggagatgc tg
//
LOCUS       DATMXK010000102         2172 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_6677, whole genome shotgun sequence.
ACCESSION   DATMXK010000102 DATMXK010000000
VERSION     DATMXK010000102.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2172)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2172)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2172
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6677"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..502)
                     /locus_tag="VN363_01865"
     CDS             complement(<1..502)
                     /locus_tag="VN363_01865"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ83279.1"
                     /translation="MSLKRLPVQVIVFTTIRIALNTMFRMVYPFLPAFGRGLGVDLQS
                     IALALSLRSAAGSASPFLVSYAESKGRRTGMLMGLFMFAAGCGLVVVYPSYVTFVLAL
                     IITLLGNFVYLSSMQAYLGDRVPYARRGLVLAVTEIGWSLSFIFGVPLAGGLISQRGW
                     SAPFPVL"
     gene            complement(511..1083)
                     /locus_tag="VN363_01870"
     CDS             complement(511..1083)
                     /locus_tag="VN363_01870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83280.1"
                     /translation="MAGIAVFTSFGPAEKTLGTNVRIVYLHGAWVWAALVSFGAAAAS
                     GLLGLLTRQFTWHTWSQALGRTGLFFWITYLPISLWAMQTNWNGLFMSEPRWRLAVIF
                     SITGLLLQIGLSLVRRPVWTSLANAIYFTVLLVVLQTTDQVLHPASPILTSEVVNIQI
                     YFISLVALTVLAAWQVARWWRCHDTLGSKA"
     gene            complement(1128..>2172)
                     /gene="hutU"
                     /locus_tag="VN363_01875"
     CDS             complement(1128..>2172)
                     /gene="hutU"
                     /locus_tag="VN363_01875"
                     /EC_number="4.2.1.49"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016153 - urocanate hydratase activity
                     [Evidence IEA];
                     GO_process: GO:0006548 - histidine catabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="urocanate hydratase"
                     /protein_id="HWQ83281.1"
                     /translation="YIDAVVDTLEEAMTMVEECVQTGTPQSIGLIGNAAEIYPELVLR
                     GVVPDVVTDQTPAHDVQMYIPAGLSVAQADSLRQSDLLQYQQRSLESMARHVEAMLAF
                     QRAGSEVFDYGNNLRQRAFDFGVKEAFNFPGFVPAYIRPLFCEGKGPFRWVALSGDPQ
                     DIYRTDEVIMELFPEDDHLQRWLRLAREKVPFQGLLARICWLGYGERAKAGQAFNELV
                     RRGEVKAPIVIGRDHLDAGSVASPNRETEAMRDGSDAVSDWAILNALINAVGGATWVS
                     FHHGGGVGIGYSQHAGQVIVADGTLEASRRLERVLTTDPGMWIVRHADAGYPEAIAAA
                     RRHGIKMPMLKDS"
BASE COUNT          567 a          649 c          566 g          390 t
ORIGIN      
        1 acaggacagg gaacggcgca ctccagccgc gttgagatat cagtccaccc gctaacggaa
       61 cgccaaatat aaagctgagc gaccagccta tttcggtaac tgctaaaacc agtccgcggc
      121 gcgcatacgg aacccgatct ccaagataag cctgcatcga agacagataa acaaagttac
      181 ctaagagggt aataatcaat gccagaacga aggtgacata ggagggataa acaacgacca
      241 acccacaccc agcagcgaac atgaacagac ccatcagcat gcctgtccgg cggcctttgc
      301 tttcggcgta ggaaaccaga aacggactgg cgctgcccgc tgccgagcgc aggctgagcg
      361 ctaatgcaat cgattggagg tccacaccca acccgcgccc aaacgcgggc agaaatggat
      421 acaccatccg gaacatggtg ttgagagcga tccggatggt ggtgaataca atcacctgaa
      481 cgggcagacg ctttagagac aaataagaat tcaggctttc gatcccagag tgtcgtggca
      541 gcgccaccac cgggcaacct gccaggctgc cagcacggtg agagccacca ggctgatgaa
      601 atagatctgg atgtttacta cctctgaagt caaaattgga gatgctggat gcaagacctg
      661 atcagtggtt tgcaatacaa ccagcagaac tgtaaaatag atcgcgtttg ccagcgatgt
      721 ccagacgggg cgccgtacca gactcaagcc gatttgcagg agcagtccag tgatagaaaa
      781 aataaccgcc agacgccaac gcggctcact cataaataag ccgttccaat tggtctgcat
      841 tgcccataac gagatcggca aataagtgat ccagaagaac aaaccggtcc tacccagggc
      901 ctgtgaccag gtatgccagg taaattggcg ggtcaataac ccgagcagcc cactggccgc
      961 ggccgcgccg aatgaaacca gggctgccca cacccaggcg ccatgcagat aaacgatcct
     1021 cacattggtc cctaaggtct tttcggcagg accaaagctg gtgaacaccg ctatcccagc
     1081 caaaataaga ccgaaccaga gcagcggcga cctccaattg ttacatttca tgaatctttg
     1141 agcatgggca ttttgatccc atgccggcga gccgcagcga tcgcttcggg atagccggca
     1201 tccgcatggc ggacaatcca catgccgggg tccgtggtca gaacccgttc caaccggcgc
     1261 gaagcctcca gcgtgccgtc agcgacgatg acctgcccgg cgtgctggct gtaaccaatc
     1321 cccacaccgc caccgtggtg gaatgacacc caggttgcgc cgccgacggc gttgatcaaa
     1381 gcattcagga ttgcccaatc gctcaccgcg tcggagccat ctcgcatagc ctcggtttca
     1441 cgattgggtg aagcaacaga cccagcatcc aggtgatccc gtccaatcac aatcggagct
     1501 ttcacctcac cacggcgcac cagctcattg aatgcctgtc cagctttggc tcgctcccca
     1561 tatcccagcc agcaaatccg cgctagcaga ccctggaagg gaactttttc ccgcgccaga
     1621 cgcagccagc gctgtaaatg atcatcctca ggaaacagct ccatgatgac ttcatctgtg
     1681 cgatagatat cctgtggatc gccggataat gccacccagc ggaagggtcc ctttccttcg
     1741 cagaagagtg gtcggatgta agccggcaca aagcccggaa aattaaaagc ctcttttacc
     1801 ccgaaatcaa acgcccgctg gcgcaagttg ttgccgtaat cgaatacttc agagccggcg
     1861 cgctggaagg ccagcattgc ttcaacatgc cgggccatcg attccagaga acgctgctgg
     1921 tattgcagca ggtcggactg gcgcagggaa tccgcctgag ccacgctcaa tccagccggg
     1981 atatacattt gcacatcgtg cgccggggtc tggtcggtga cgacatcggg gacaaccccg
     2041 cgcagaacca gctcgggata aatttcagcc gcattgccaa tcaagccgat ggattgcggc
     2101 gtaccggtct ggacacattc ctccaccatg gtcatggctt cttccagcgt atcgacgact
     2161 gcatcaatat ac
//
LOCUS       DATMXK010000103         4139 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_163099, whole genome shotgun sequence.
ACCESSION   DATMXK010000103 DATMXK010000000
VERSION     DATMXK010000103.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4139)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4139)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4139
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163099"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..425)
                     /locus_tag="VN363_01880"
     CDS             complement(<1..425)
                     /locus_tag="VN363_01880"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83282.1"
                     /translation="MLRILLADDHVLFRKGLTALLSARPDIQIVGEARDGLEAIEAAR
                     EFLPDVILMDVNMPRCDGLEATRRIKREMPHVKIVILTVSDDDQHLFEAIKSGAQGYL
                     LKDLEPYQLYDLLESISRGEAPLSGAIAAKILKEFSHPNG"
     gene            complement(447..3050)
                     /locus_tag="VN363_01885"
     CDS             complement(447..3050)
                     /locus_tag="VN363_01885"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ83283.1"
                     /translation="MCIYGSDPGGSPPSGCGAYGTACIGIRQVDQSTHLIISDSQNEV
                     PGWFQKPGALGRAIRSKIRMSLGARLIWILVGVSLISILLSSYLLFDYQHQQFVAEQK
                     DAITLLSSTLESSLRQAMLSGEPEQVNSMIQMVASEPEIISLRILDLQGEVQFSSQKA
                     EVGTRLDLRQQTCQLCHAGALPEKNATAITEVHSGEQVLINGNLIPNTSECHACHDPQ
                     ADSLGVMLIEASLAGANQHQISSFWRSGAISLLSVVLLVALVGTALNRYVHKPLSNLS
                     QGVAEISAGNLDEPVMMTHQDEIGDLVIAFNDMRVKLKQSYQEQEKHRQDMAVMNEVA
                     LAATQLLDVQDILNFTLDTMVDRLGMLAGLIFLWDDATKRYTPQATRNISQDQVDEIE
                     RRRKAGWDITREVADSGQEIVIYDIPQDYRFHGLWDDRTGQAYIKVPMMSRGTVVGVL
                     SLISPAEKTVTQPGVEYLKAIGREVGIAIDNALLLKNTRQGEKEANALYKLGSKISAS
                     LALDEVLDAVAEAARELLEADIGLVGLFEEESQEVVIMAVAGDQGVTLKGARVPVHSQ
                     APGNVLLKGQPIIANASVGDALKVNAEDATTDETIASFLAVPLERGEHFLGLVEVMIR
                     PPLTFRDNDAQLLMRLATQVVVAIENAQLYLQLRHMAALQERDRLAREMHDHLAQSLG
                     YINVRAAMTDELLSAGKAEQALENIRELKRAAKIAYTDVREEIFNLRTTVASPDNFFE
                     TLKNYLSEYRTHYGVIVELTVDAAELCDFDAETGSQLLRIIQEALGNVRKHSGASLVR
                     IHFHEEASQVLVCIEDNGEGFDLERVPQASGQHYGLQIMRERAESVGGSLTLESAPGQ
                     GTRVIVCVPGV"
     gene            complement(2978..4066)
                     /locus_tag="VN363_01890"
     CDS             complement(2978..4066)
                     /locus_tag="VN363_01890"
                     /inference="COORDINATES: protein motif:HMM:NF022077.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Mrp/NBP35 family ATP-binding protein"
                     /protein_id="HWQ83284.1"
                     /translation="MASIEEIKQALTRVIDPELGKNVVELGMIRDIQLADGQVKILLA
                     LTIAGCPMSNQLRSQTQAAAESVPGVKNVTVELTSMTPEERQKIHSRAPSRAPQALNL
                     NQIGRIVAVLSGKGGVGKSSVTSLLATALQRQGLTVGILDADITGPSIPRMFGITGPV
                     PSVPFGILPIKSSSGVRVISTNLMVPEEDTAVVWRGPVVSNTIKQFWSDVLWGRLDYL
                     LIDLPPGTSDATLTVMEALPLDGVVLVTMPQGLSSMVVRKTVHMAQGLKVPILGIVEN
                     MSYFVSPETGTRFEIFGPSQTDKVTASANAPLLGCLPIDPHLAELADTGQIEAYTHPA
                     YDALACAFMEAIPAVAPRPAAARMAQPA"
BASE COUNT          909 a         1205 c         1066 g          959 t
ORIGIN      
        1 ccattggggt gggagaattc ttttaagatt ttggcagcaa tcgcaccgga aagcggtgct
       61 tcgcctcgcg agatgctttc cagcaagtca tatagctgat atggctcaag gtccttgagc
      121 aggtaacctt gcgctccgct cttgattgct tcgaacaaat gctggtcatc atccgagacc
      181 gtcaggatga cgatcttgac atgcggcatt tcgcgcttaa tccgccgggt cgcttccagg
      241 ccatcacagc gcggcatgtt gacatccatc aggattacat cgggtagaaa ttcccgcgcc
      301 gcttcaatag cttccagacc atcccgggct tcaccgacga tctgaatatc cggacgagcg
      361 ctgagtaatg cggtcagacc cttccggaac agaacatggt cgtcagccag taaaatcctc
      421 aacatcttct catctccttc caccctctaa acgcctggca cgcacacgat cacccgtgtc
      481 ccctgcccgg gcgctgactc taacgtcagg ctgcctccaa ccgactcggc ccgctcgcgc
      541 atgatttgca atccataatg ctgtccactg gcttgcggaa ctcgctcgag atcaaatcct
      601 tctccattat cctcgataca aaccagcacc tgagaagctt cttcatgaaa gtggattcgc
      661 accaggctgg cgccggagtg cttacgcaca tttccgagag cttcctggat gatacgtagt
      721 aattggctac cggtctctgc gtcgaaatca cacagctctg cggcatcaac cgtcaattct
      781 acaatgactc cgtagtgggt ccggtattcc gaaagatagt tttttaatgt ttcgaagaag
      841 ttatccggag atgcaactgt tgtgcgcaga ttgaatattt cttcgcggac atccgtatac
      901 gcaattttag cggctcgctt gagctcacgg atattctcca gggcctgctc tgcttttccc
      961 gcactcaaaa gttcgtcggt catcgctgcc cggacattga tataacccag agattgggca
     1021 agatgatcgt gcatttcgcg cgccaggcga tcacgctcct gtaaagccgc catatggcgg
     1081 agctgcaggt aaagctgggc attttcgatc gccaccacaa cctgggtcgc caatcgcatc
     1141 aataattgag catcgttatc acggaaggta agcggcgggc ggatcatcac ctctaccaga
     1201 ccgaggaaat gctcaccacg ctccaatgga acagccagga aggaggcgat cgtttcatcg
     1261 gttgtcgcgt cctcagcgtt cacctttaag gcgtccccca ccgaagcatt ggcaattatg
     1321 ggctgcccct tcagaaggac attgcccgga gcctggctgt ggacgggcac tctggcgcct
     1381 tttaatgtaa ccccctggtc gccggcgact gccataatga ccacttcctg ggattcttct
     1441 tcgaacagcc ccaccagacc gatatccgcc tcaagcagct cccgcgccgc ttcggcgacg
     1501 gcatccaata cttcgtcaag cgccagcgag gctgatatct tactcccaag tttgtaaagg
     1561 gcattggctt ccttctcgcc ctgacgggta ttcttcagaa gcagggcgtt atcgatggcg
     1621 atgcccacct cgcgtccgat cgccttcaaa tactccactc ccggctgggt taccgtcttt
     1681 tcagccggcg agatcaggct cagcacaccg accacagtcc ctcgcgacat cattggcacc
     1741 ttgatatacg cctgtcccgt gcgatcgtcc cacagcccat gaaagcggta gtcctgagga
     1801 atatcataga ttacgatttc ctggcccgag tcagccactt cgcgggtgat atcccacccg
     1861 gctttgcgcc ttctttcaat ttcgtctacc tgatcctggg agatatttcg ggtcgcctgg
     1921 ggggtatagc gtttcgtcgc atcgtcccac agaaagatca atcccgccag catgcccagc
     1981 cggtctacca tagtatccag ggtgaagttc aagatatcct gaacatccag taattgggtg
     2041 gcagccaggg caacttcgtt catcaccgcc atatcctgcc ggtgtttttc ctgctcctgg
     2101 taagattgct ttaatttcac ccgcatatca ttgaaggcga tcaccagatc accgatctcg
     2161 tcctggtgag tcatcatgac gggctcatcc aaatttccgg cgcttatctc agccactccc
     2221 tgggaaaggt tggagagcgg tttatgcaca taccggttta atgcggtccc gaccagggca
     2281 accagtaaaa caacactcag cagactgatc gctcctgaac gccagaaact gctgatctga
     2341 tgttgatttg ctccagccag ggaggcttcg atcagcatca cccccaatga atcggcctgg
     2401 ggatcatgac aggcatggca ctccgaggta ttggggatca gattcccgtt gattaatacc
     2461 tgttcacctg aatgtacctc ggtgatggcg gttgcattct tctctggcag ggcgccagca
     2521 tgacacagct ggcaagtttg ctggcggaga tccaggcggg ttcccacctc cgctttttgg
     2581 ctgctgaact gcacttcccc ctgcaaatcc aatatgcgta agctgataat ttcaggctcg
     2641 gaagcaacca tctggatcat gctgttaacc tgctccggct cgccgctgag catcgcctga
     2701 cgcaggctgg attccagggt gctgctcagc agcgtaatgg catctttttg ttcggccacg
     2761 aactgctggt gctggtaatc aaacagcaga tagctcgaca acaaaatact gatcaaagat
     2821 acaccgacca gaatccagat cagccgggcg cccagggaca ttctgatctt cgagcggatg
     2881 gcgcgcccta aggcgcctgg tttttgaaac caaccaggca cctcattctg ggagtcggaa
     2941 ataatcagat gtgtgctttg atccacctgg cgtataccta tgcaggctgt gccatacgcg
     3001 ccgcagccgg acggggggct accgccggga tcgcttccat aaatgcacat gccagagcat
     3061 cgtaggctgg atgggtatag gcttcgatct gcccggtatc ggccagctcg gccagatgag
     3121 ggtcgatcgg caggcagccc agcagcggcg cattggcgct ggcagttact ttatctgtct
     3181 ggctgggacc gaaaatctca aagcgtgtcc cggtttccgg cgagacaaaa tagctcatat
     3241 tctctacaat acccaggatc ggaaccttca atccctgggc catatggacg gttttacgca
     3301 caaccatgga cgataagcct tgcggcatgg tcacgaggac cacgccatcc agaggtaagg
     3361 cttccatgac cgtcagggtc gcatcggagg tgccgggggg taaatcgatc agcagatagt
     3421 cgagtcttcc ccaaaggaca tcgctccaga actgtttgat cgtattgctg accacggggc
     3481 cgcgccatac aactgcagta tcctcttccg gaaccataag gttggtggag atcacccgca
     3541 ccccggacga gctcttgatg ggcaggatcc cgaatgggac gctggggaca ggaccggtaa
     3601 tcccgaacat gcgcgggatg ctgggtccgg tgatgtcggc gtccaatata cccaccgtta
     3661 atccctggcg ctggagagca gtcgccagca agctggtgac cgaggatttg cccaccccac
     3721 ccttaccgct caggacggca acgatgcggc ctatttgatt caaatttaat gcttgaggtg
     3781 ctcgggaggg agcacggctg tgaattttct ggcgctcttc aggagtcatg ctggtcagct
     3841 cgacggtgac attcttaaca cccggcacag actcagctgc ggcctgagtc tgactgcgga
     3901 gctggttgct cattggacag ccggcgatcg tgagggctaa caggatcttt acctgaccat
     3961 ccgctaattg aatatcgcgg atcatcccca gctcgacaac atttttacca agttcagggt
     4021 ctatgacccg cgtcaacgct tgtttgatct cttcaatcga tgccatgtat tatgctcttt
     4081 ctttcggtga gatcgcctgc gggaggatac ctgtaaccgg accaatacca accgggaac
//
LOCUS       DATMXK010000104         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_1940, whole genome shotgun sequence.
ACCESSION   DATMXK010000104 DATMXK010000000
VERSION     DATMXK010000104.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1940"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..541)
                     /locus_tag="VN363_01895"
     CDS             complement(<1..541)
                     /locus_tag="VN363_01895"
                     /inference="COORDINATES: protein motif:HMM:NF025157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Fic/DOC family N-terminal domain-containing
                     protein"
                     /protein_id="HWQ83285.1"
                     /translation="MIKVGQGQASYWAFSPDPLSPEIRSDWNLTGALSQADRAVSELA
                     GLGIMLPNPALYIHPFVRREAVLSSRIEGTQSDLTDLYLYEGQQLMLPGMPTADPATS
                     DVREVFNYVRALDHGLERLSSLPLSLRLIREVHRVLMQGVRGEHATPGEFRTSQNWIG
                     GATINQAVFIPPPVDVMRTA"
     gene            complement(582..>3125)
                     /locus_tag="VN363_01900"
     CDS             complement(582..>3125)
                     /locus_tag="VN363_01900"
                     /inference="COORDINATES: protein motif:HMM:NF012403.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWQ83286.1"
                     /translation="DEVGLGKTIEAAMVFEELKLRRQVRRALFVVPAGLTHQWRDEFE
                     QKFGEKFILYDRELFSALREIHGREANLWKFNDQVITSLDFIKPRRIRPGLSAEEKAR
                     REEHNRLVFQHLQEAGWDLAVFDEAHKLSKHGDGSETTRYQVGEALARSVPVVLLLTA
                     TPHQGDPGHFLHLLNLVDAYGFQNITDLRPDRVAEVVWRTRKRAAVDANGKQLFKGRI
                     TDIYPVDRSGPEHTLERQLYDEVTDYVREHYNQAMQRGDRAFGFLMILFQRLVTSSSA
                     AIAEALTRRLERLTALRTRLTQETVPDSREDYDEQAQQDEDAQASLDELIHLAGVVDL
                     AELQGEIGILERLLDLARQARRQHDAKVIALLQIIDEVRRREGPLTKFLVFTEFVATQ
                     KALRQILEGLGYSVTTISGSQGLEERITARKAFAEQAQFLISTDAGGEGINLQFCHVM
                     VNYDLPWNPMRLEQRIGRLDRIGQTRDVLVLNLLISDTVEARVREVLEEKLGLIRKQF
                     GDDKLADILSTLQEEFQFDRLYMDAVMRNAAHSAELEHLAQQIFDRARQVLEKDDLLL
                     PQSQAQMEQFRQRLVESAQPRLRAMLEGYLAARGEQLHEYSRRPGIFYFDLLEPDGFR
                     IHYPDVAFDRSLAVGDDSLDYIHLNHPLIQDILAELTTGKQAGLTLLRLKQEALSAGL
                     ISGADPAVWALYRLRYTNYLDVDRQELIPVAVDAAGQSLPRLARVVLELTPEQVEAVF
                     VPPTGLNISGLLEQARKLAEVQAGDRFSEAQVMQSERLQAERRKIEKYYRQQEAAVGQ
                     IAIENIRQAKQRELLERRHSDLESLDKRLQLVPDMNLVGMALLTFEKVL"
BASE COUNT          651 a          945 c          825 g          704 t
ORIGIN      
        1 atgctgtgcg catcacatcc actggcggtg ggatgaatac agcctgattg atcgtggccc
       61 cgcctatcca gttttgcgag gtgcgaaatt cgccgggagt ggcatgttca ccgcgcacac
      121 cctgcatcaa cacccggtgt acttctcgaa ttaaccttaa actgagcggc agagatgaca
      181 gtcgttctaa tccgtgatcc agggcacgta catagttaaa tacttctcga acatctgaag
      241 ttgcagggtc agcagttggc ataccgggca acattaattg ctggccttca taaagatata
      301 aatccgtcag atcggattga gttccttcga tgcgtgagga taacactgct tcacggcgga
      361 caaaaggatg gatgtatagt gccgggttgg gtagcattat tccaaggcct gccagttcac
      421 tgaccgcacg atcggcctgc gatagggcac cggtcaggtt ccaatcggat cgaatttctg
      481 gcgaaagtgg atctggactg aaagcccaat aagaagcctg gccctgtcca acttttatca
      541 actgtcctgc tgctttactt tgaaaggttt ttgggtccat cttataacac cttttcaaac
      601 gtcaggagcg ccatcccaac caggttcata tcgggaacca actggaggcg cttatccagg
      661 gattccagat cgctgtggcg tctttcaagc agttcgcgct gttttgcctg gcggatattc
      721 tcgatggcaa tctgccccac ggcggcttcc tgctgtcgat aatatttttc aatcttccgc
      781 cgttcggcct gcaggcgctc ggactgcatt acctgcgcct cgctgaaacg gtctccggcc
      841 tgcacctctg ccagcttacg tgcctgctcg agcagtccgg atatattcag cccggtcgga
      901 ggtacaaaga ccgcctcgac ctgctctggc gtcaattcca gtaccaccct ggcaaggcgc
      961 ggcaaagact gccccgctgc atccacggct accgggatca attcctggcg gtccacatcc
     1021 aggtaattgg tgtaacgcaa ccggtacaat gcccatactg ctggatctgc acctgaaatc
     1081 aacccggctg atagtgcctc ctgcttcagg cgcagcaggg tcagtccggc ctgcttcccg
     1141 gtggtcagtt cagccaggat atcctggatc aggggatggt tcaggtgaat ataatccagg
     1201 ctgtcgtctc caaccgccag gctgcgatca aaggctacat ccgggtaatg gatgcggaag
     1261 ccatctggct caagaagatc gaaatagaag atccctggac gcctgctgta ctcatgcaac
     1321 tgctctccac gcgccgccag atagccctcc agcatggccc gcaggcgagg ctgggcgctc
     1381 tcgaccagac gctggcgaaa ctgctccatc tgcgcctggc tttggggcag cagcaaatcg
     1441 tctttctcca gcacctggcg ggcgcggtca aatatttgct gcgccaaatg ctcgagttcc
     1501 gccgaatgcg ccgcgttgcg catcaccgca tccatataca ggcggtcaaa ctggaattcc
     1561 tcctgcaggg tcgagaggat atctgccagc ttatcatctc cgaactgttt gcggatcagc
     1621 ccgagctttt cctccagcac ctcccgcacg cgcgcctcga ccgtatcgct gatcagcagg
     1681 ttcagcacca gcacgtcccg ggtctggccg atgcggtcca ggcggccgat gcgctgctcc
     1741 agcctcatcg ggttccaggg cagatcgtaa ttgaccatca catggcagaa ttgcaggttg
     1801 atgccctctc cccccgcatc ggtggagatc aggaactggg cctgttctgc aaaagccttg
     1861 cgggcagtga tccgttcttc cagtccctgt gagccgctga tcgttgtcac actgtagccc
     1921 agtccttcca ggatctgacg cagcgccttc tgggtggcca caaactcggt aaagaccaga
     1981 aatttcgtca gcggaccttc ccgccggcgc acctcatcga taatctgaag caaggcaatc
     2041 accttggcgt catgctggcg gcgcgcctga cgggccaggt cgagcaggcg ctccaatatg
     2101 ccgatctccc cctgcagctc cgccagatcc accactccag ccaggtggat cagctcatcc
     2161 agggaggcct gtgcatcctc atcctgttgc gcctgttcat cgtagtcttc ccgactatct
     2221 ggaacagtct cctgtgtcag acgggtgcgc agggctgtca gccgttccag ccggcgggtc
     2281 agtgcttcag caatggcagc gctgctgctg gtcaccagac gctggaaaag gatcatcagg
     2341 aagccgaatg cccggtcgcc gcgctgcatg gcctggttgt agtgctctcg cacataatcc
     2401 gtgacttcat cgtagagttg ccgttcgaga gtgtgctctg gcccggaacg atccaccgga
     2461 taaatatccg taattcttcc cttaaacaac tgtttaccat ttgcatccac agcagctcgc
     2521 ttgcgcgtac gccagaccac ttcagctacc cgatccgggc gcagatccgt aatattctga
     2581 aagccgtagg cgtcaaccag gttcagcaga tgcagaaaat gaccggggtc gccctggtgg
     2641 ggggttgccg tgagaagcaa cacaaccggc acgctcctgg caagcgcctc gcctacctgg
     2701 tagcgtgtgg tttcgctgcc atcaccgtgt ttgctcaatt tgtgggcttc gtcaaagact
     2761 gccagatccc agcccgcctc ctgcaggtgc tgaaaaacca gacggttgtg ttcttcacgc
     2821 cgggcttttt cttccgccga aagccccggg cgaatgcggc gcggtttgat gaaatccagg
     2881 ctggtgatca cctgatcgtt gaatttccac aggttggcct cccggccatg gatttcgcgc
     2941 agtgccgaaa ataattcgcg gtcgtacagg ataaacttct cgccgaattt ttgttcgaac
     3001 tcatcccgcc attgatgtgt caaccctgca ggaaccacaa acagagcccg tcgaacctga
     3061 cgacgtaact tcaattcctc aaaaaccatg gcggcttcaa tggtcttacc cagacctacc
     3121 tcatc
//
LOCUS       DATMXK010000105         4827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_46794, whole genome shotgun sequence.
ACCESSION   DATMXK010000105 DATMXK010000000
VERSION     DATMXK010000105.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46794"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..353
                     /locus_tag="VN363_01905"
     CDS             <1..353
                     /locus_tag="VN363_01905"
                     /inference="COORDINATES: protein motif:HMM:NF014647.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ83287.1"
                     /translation="DTLFVYANQVGLGSIQAAKEQGVKFVGFSGDQNSVAPGTVVASV
                     AFDFETFYTWTLDKFIKGELDGNTVHMAGIKEGIFRPVYTDQTGSDIQAKVQAGIDKV
                     TNGEIDLTTMFANP"
     gene            477..2015
                     /locus_tag="VN363_01910"
     CDS             477..2015
                     /locus_tag="VN363_01910"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83288.1"
                     /translation="MPAVPFVELKNISKYFAKVIANQDVSMQIRRGEVLALLGENGAG
                     KSTIMKVLYGLYKADTGQILVDGQEKVINSPRDAMALGIAMIQQHFSLVQAHTVTENI
                     ILGNVQGRYDLQKYDRVIRDLASRYGFELEPSALISDLPVGVQQKVEILKALYQNAQL
                     LIMDEPTAVLTPQESDVLMKFVRENTREGKAVVFITHKLREVMEVADHIVVMRNGRVV
                     GDLRKDETNELELSKLMVGKEIHERTYIEDFDYEKSPVGLELQNVTIRESREDVARIK
                     DINFQVHKGEILGIAGVSGNGQDALCDAITGYRELDGGRILLNEKDISAQSVRERIQL
                     GISYVPSDRQRYGLIMALKLAENMLLKSSFEDRWVRRGLIDQKALDAYTKEKIQDYQV
                     KATGPDQVVRNLSGGNQQKVILAREVDLGREVIVFDQPTRGLDLGAIDHIQHTILAEK
                     KKGKAILLISTELTEIFALSDRIAVIYKGEIVGIYRNRDLDTEQIGLLMAGYAPEREQ
                     PHEP"
     gene            2053..3069
                     /locus_tag="VN363_01915"
     CDS             2053..3069
                     /locus_tag="VN363_01915"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83289.1"
                     /translation="MAGLVASGLLLLFLGENPLTAMGTLFGSLFRDGYTFADIFVKAT
                     PLIFTALAFAFPYKANLYNIGAQGQFYIGSITAVALSLILAGSLPGSVALVVIALMTI
                     LVGGLWGVLIGWLKARFNANEFLVSMMSTYVALNIMNYLLRTFLMETKAEYPQTNPLD
                     PDIWLPVMLRGTRLHVGFLLAVLACIGVWVLLYRTPLGFRIRAVGSNKDAAEMAGISS
                     RLITIVTFFIAGALAAMGGFAEVNGVQHMLVQGFNPDIGSAGIGIAILANGHPIGIIF
                     AAILFGALKVGGTIMGQLSGIPASIIELMQGFVMVFVILSYFVREVLEVRREKARLKD
                     AVSA"
     gene            3066..3980
                     /locus_tag="VN363_01920"
     CDS             3066..3980
                     /locus_tag="VN363_01920"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83290.1"
                     /translation="MIELLVRTIMMGTPLLYGSLSEVYSERSGVMNTAIEGGFLMGAW
                     AGFVAAYLTGGNLLIGLLAAMLAGILTAALYGWITIYLKQHQIVTGVAINILAIGVCA
                     FFQRVIFGVPITPLIVQPFQTIPIPLLSDIPLIGPILFNQNILTYLLFLLVPAANFIL
                     FKTSVGLSIRSIGENPEAGDVAGLRVDRIRFLTILFAGLMCGIGGSFYSIGYLGMFTT
                     TIIGGRGWIAFAICFLGNWRPLGALAGTLVFGLAEAVAIFMQSSGSDLFPNELFIALP
                     YLLTIVLTAMRRSFNVPAQLGVPYKKEG"
     gene            complement(4054..>4827)
                     /locus_tag="VN363_01925"
     CDS             complement(4054..>4827)
                     /locus_tag="VN363_01925"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWQ83291.1"
                     /translation="SLLSGALLGAIFIVTLGAAWLSERTLPPPAGEARSLKVMTYNIQ
                     QANDENAQRAHLRQLELIRQVSPDVLALQESDSARISLGNNDYVRYYAGKLGYYSYYS
                     PKTVTGTFGAALLSRYPLENPHTVFTFSDQDEIGTTVAEIQVGDRRFNIYNVHPDGSD
                     SAMLVFAEELLRRTAGQENVIALGDFNLRPYEEAYQLIDAVLINAWTDAFPDGGTMPE
                     ERRIDHIFVSPGLEVRDPVYLLAPESATDHPAHWAEIGW"
BASE COUNT          989 a         1358 c         1415 g         1065 t
ORIGIN      
        1 ctgataccct gtttgtgtac gccaaccagg tcgggctggg ctcgatccag gcagccaagg
       61 agcagggcgt caagttcgtc ggcttctctg gcgatcaaaa ttcggttgct ccgggtacgg
      121 tcgttgcctc cgttgcattt gatttcgaaa ccttctatac gtggacgctc gacaagttca
      181 tcaagggcga gctggacggc aataccgtcc acatggcagg catcaaagag ggtatcttcc
      241 gaccggtcta caccgaccag accgggagcg atattcaggc taaagtccag gccgggatcg
      301 acaaagtgac caatggcgag atcgacctga cgacgatgtt cgctaatccg taagccaggc
      361 ggttcaaaag ataccttttc aggttcaggc tgaaaaggta tctttttttt ctgcaaagta
      421 tgagagctga aacctgctct ccatcacctg caaccttcca ggaaggatgt tttaatttgc
      481 cagcagttcc gttcgtcgag ctgaagaata tcagtaagta ttttgccaag gtcatcgcca
      541 accaggatgt ctcaatgcag atccgtagag gcgaggtcct cgctctgctg ggggagaatg
      601 gcgccggaaa aagcacgatc atgaaagtgc tctacggtct gtataaagcc gatacagggc
      661 agatactggt ggacgggcag gaaaaggtaa ttaactctcc cagggatgcg atggcgctgg
      721 ggatcgctat gatccagcag catttttcgc tggtccaggc ccacacggtc actgaaaaca
      781 ttatcctggg taacgtgcag gggcggtacg acctccaaaa atacgaccgg gtgattaggg
      841 acctggcgtc tcgctatggg ttcgagctcg agccgtccgc actgatcagc gacctgccag
      901 taggcgtgca gcagaaggtg gagatcctta aggcgctgta tcagaacgcc cagctcctga
      961 tcatggacga gccgacggcg gtgctgacgc cccaagaatc cgatgtgctg atgaagtttg
     1021 tccgggagaa tacccgcgag ggtaaagctg tcgtctttat cacccacaaa ctgcgcgaag
     1081 tgatggaggt tgccgatcat atcgtcgtga tgcgcaacgg ccgagtcgtg ggtgatctgc
     1141 gcaaagacga aacgaacgag ctcgagctgt ccaagctgat ggtcggcaaa gagatccatg
     1201 aacgcaccta tatcgaagac ttcgattatg aaaagtcacc ggtaggcctg gagcttcaaa
     1261 atgtcaccat ccgggagagc cgagaggatg tcgcccgcat aaaggatatc aacttccagg
     1321 tgcacaaagg cgaaatcctg ggcattgccg gagtcagtgg aaacgggcag gatgctctat
     1381 gcgatgcgat caccggctac cgtgaattgg atggagggcg gattctgctc aacgaaaaag
     1441 acatcagcgc ccaaagcgta cgggagcgaa ttcagctggg gattagctat gtcccctccg
     1501 accgccagcg ctacggcctg atcatggccc tcaaactggc cgagaacatg ctcttgaagt
     1561 cgagctttga ggaccgctgg gtgcggcgcg ggctgatcga ccagaaagcg ctcgatgcct
     1621 acactaaaga aaagatccag gactaccagg tcaaggctac cgggcctgat caggtagtcc
     1681 ggaacttgtc gggcggcaac caacaaaaag tgattctggc ccgcgaggtc gatctgggac
     1741 gggaagtgat cgtcttcgac caacccacgc gcggcctgga tctcggcgct attgaccaca
     1801 tccaacacac catcctggct gagaaaaaga aaggcaaggc aatcctgctg atctcgacgg
     1861 aactgactga aattttcgcc ctgtccgacc ggattgcggt catctataaa ggagagatcg
     1921 ttggtatcta ccgcaaccgt gacctggaca ctgagcagat cgggctgtta atggccggct
     1981 atgcaccgga gagggagcaa ccccacgaac cgtagactaa gagacctcct gcttaccaat
     2041 ctcctggcga tcgtggcggg actggtagcc agcgggctgc tgctgctatt cctgggtgaa
     2101 aaccccctaa ctgccatggg aacgctgttc ggatccctct tccgggatgg atataccttt
     2161 gccgacatct tcgttaaagc tacgccgctg atattcacag cgctggcctt tgccttccca
     2221 tacaaggcca atctgtacaa catcggcgcg caagggcagt tctatatcgg ctcgattact
     2281 gcggtggctc tgtcgctgat cctggcgggc agcctgccag gatccgtagc gctggtcgtc
     2341 atcgctctga tgaccattct ggtaggtggt ctgtggggcg tgctcatcgg ctggctcaaa
     2401 gcacggttca atgccaatga attcttggtc agcatgatgt ccacctatgt ggccctcaac
     2461 attatgaact acctgctgcg cacctttctg atggagacca aggccgagta cccgcagacg
     2521 aacccgctcg accccgacat ctggctgccg gtcatgctgc gcggcacccg gctgcacgtc
     2581 ggctttctgc tggccgtgct ggcctgtatt ggcgtctggg tgctgctgta ccggactccg
     2641 ctcggattcc gcatccgggc agtgggtagt aacaaggatg ccgctgaaat ggccggtatc
     2701 agctcccggc tgattacgat cgtaactttc tttatcgctg gagctctggc ggccatgggc
     2761 ggatttgccg aagtcaatgg cgtccagcac atgcttgtcc agggtttcaa tccggatatc
     2821 ggctcggccg ggattggcat cgccatcctg gcgaacggtc acccgatcgg gattatattc
     2881 gcggcaatcc tgttcggcgc gctgaaggtg ggtggcacga ttatgggcca gctctcggga
     2941 atccccgcca gcattatcga gctgatgcag ggctttgtca tggtttttgt gatcctatcc
     3001 tatttcgtcc gtgaagtgtt ggaggtccgg cgcgaaaaag ctcgcttgaa agatgcggtg
     3061 agcgcatgat cgagctgctg gtccgcacca ttatgatggg cacgcctctg ctttacggtt
     3121 ccctttccga ggtgtattcc gagcgcagcg gcgtgatgaa tacggcaatc gaaggcggct
     3181 ttctgatggg cgcctgggct ggctttgtag ccgcgtatct gaccggcggt aacctgctga
     3241 tcgggctgct cgcagcgatg ctggccggta tcctgaccgc cgccctgtac ggctggatca
     3301 ccatctacct gaaacagcac cagatcgtca ccggtgtggc gatcaacatc ctggccatcg
     3361 gcgtatgcgc attcttccag cgagtgatct tcggtgtgcc aattacaccc ctgatcgtac
     3421 agccgttcca aactatcccc atcccgctgc tgtcggacat tccgctgatc ggcccgatct
     3481 tgttcaacca gaatatactg acttatctgc tgttcctatt ggtgcctgcg gccaatttca
     3541 tccttttcaa gacctcagtc gggctgtcca tccgctccat cggcgagaat ccggaggcgg
     3601 gcgacgtggc cggtttgcgg gtggaccgca tccgtttcct gacgattctc ttcgcgggtc
     3661 tgatgtgcgg gatcggtggc tcattctatt cgattggcta cctggggatg ttcaccacca
     3721 ccatcatcgg tgggcgcggc tggatcgcct tcgccatctg tttcctgggg aactggcggc
     3781 cgcttggcgc tctggccggg acgctggtct tcggtctggc cgaagccgtg gccatcttca
     3841 tgcagtctag cggcagcgac ctgtttccca acgagctttt catcgccctg ccgtacttgc
     3901 tgacaatcgt gctgaccgcc atgcgccgct cgttcaatgt ccctgcccag cttggggtgc
     3961 cttataagaa ggaagggtag agaagttctg accccaacgg ccgactctgt caccatttaa
     4021 aaggaaaaaa tctgggggct atgagcaggt gcgttaccaa ccgatctcgg cccaatgtgc
     4081 cgggtggtcg gtggccgatt ccggtgccaa taggtacacc gggtcgcgca cttccagccc
     4141 cggcgagacg aagatatggt cgatgcgccg ttcctcgggc attgtcccgc catctggaaa
     4201 cgcgtctgtc caggcgttga tcaataccgc atcgatcagc tggtacgcct cttcgtacgg
     4261 gcgtagattg aagtccccca gggcgattac attctcctgg ccggccgtgc ggcgtaataa
     4321 ttcctccgca aataccagca tggcgctgtc cgagccatcc gggtggacat tgtagatatt
     4381 gaagcgcctg tccccgacct ggatttcggc gaccgtggtg ccgatctcgt cctggtcgct
     4441 gaacgtgaac actgtgtgcg gattctcgag cgggtagcgc gatagcagcg ctgcgccgaa
     4501 ggtgccggtg accgtcttgg ggctgtaata gctgtagtag cccagcttgc cggcatagta
     4561 gcgcacatag tcgttgttgc ctagcgagat gcgcgccgag tcgctttcct gcagcgccag
     4621 cacgtccggg ctcacctgcc ggatcaattc aagctggcgc aggtgggcgc gttgcgcatt
     4681 ctcgtcattg gcctgctgga tattgtaggt catcactttc aggctgcggg cctctcccgc
     4741 cggcggcggc agggtgcgct cgctcagcca ggccgcgccc agggtaacga tgaaaatcgc
     4801 tcccagcaac gcgccgctga gcaagct
//
LOCUS       DATMXK010000106         2114 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_139818, whole genome shotgun sequence.
ACCESSION   DATMXK010000106 DATMXK010000000
VERSION     DATMXK010000106.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2114)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2114)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2114
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139818"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2114
                     /locus_tag="VN363_01930"
     CDS             <1..>2114
                     /locus_tag="VN363_01930"
                     /inference="COORDINATES: protein motif:HMM:NF013126.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycoside hydrolase family 3 N-terminal
                     domain-containing protein"
                     /protein_id="HWQ83292.1"
                     /translation="QHFVGGVVLSAENDNFTDADNVLTQTQALTRQLQSNQWAIAQQN
                     RVDPVSGATYLPPFVPMFIGLSQEGDGYPFDQILSGLTTLPNLMAVGATWNPDLAGEI
                     GQIVGTELQSLGINLLIGPSLDILENTYAEGKNNLGTRTFGGDPFWVSELGKAYISGV
                     HTSSNGRVAVAATHFPGHGGSDRLPEEEVPTVRKSLDQLKDFELAPFYAVTGNAPSSD
                     ATVDALLVSHIRYQGFQGNIRATTRPVSFDPQAFNLLMQLPPITAWRSNGGLMISDNL
                     SSQAVRRFYELTSQTFDARRVALNAFLAGNDLLYVADFTSSELPESMEATIQTLQFFA
                     DKYREDPAFAQRVDESVLDILTLKLKLYGDFTLGLTLPRVEDLAKIGRSSSVVFDVAR
                     ESATLISPSAQELDDLLPDAPNRNDRMVFITDSRPAKQCTGCTTSETLPVDALEEVIV
                     RRYGTAGGGQITPAYLTSYSINDLQTMLAGLPGTTQMDVDLSRAQWIVFALQDNSPQY
                     LAYQTLSQFLSERPDLFQQKRLIVFAFNAPFYLDATNITKLTAYYALYSKAAAFIDMA
                     SYLLFREIPATGALPITVPGVYDLNAALFPDASQILQVMVDSPTGSPTQGTGTPDPAP
                     ALEFAVGETLSLRTGVVRDNNGNPVPDGTPVDFILNQGGETRQPAFTVNGIARLAFII
                     NQPGVLEVRVETETARQSNILH"
BASE COUNT          463 a          641 c          540 g          470 t
ORIGIN      
        1 accagcattt tgtgggtggc gttgttctat ctgcagaaaa cgataatttc acggatgccg
       61 ataacgtcct cactcaaacc caggcgctca cccgccagct gcagtcgaac cagtgggcga
      121 tcgcccagca aaatcgggtc gacccggtga gcggtgcgac ttacctccca ccttttgttc
      181 ctatgttcat cggcctatcc caggagggag acgggtaccc ctttgaccag atcctgagcg
      241 ggctcaccac actacctaac ctgatggcag tcggtgcaac ctggaacccg gatctggcag
      301 gtgagatcgg acaaattgtt ggcacagagc tgcagagcct gggtatcaac ctgctcatcg
      361 gtccttctct ggacatttta gagaatacct acgccgaagg taaaaacaac ctggggacgc
      421 gcacatttgg cggcgatcca ttctgggtct cggaactggg taaagcatac atttcggggg
      481 tgcacaccag cagcaatggg cgcgtggcgg tcgccgcaac ccacttccca gggcatggcg
      541 gctcagatcg cctgccggag gaagaagtgc ctaccgtccg caagtccctc gaccagctga
      601 aggactttga gctggctcca ttttacgcgg taaccgggaa tgccccctca tccgatgcga
      661 ccgttgatgc gctgctcgtt tcccatatcc gctaccaggg atttcagggc aatatccggg
      721 ctaccactcg ccctgtcagt tttgacccac aggccttcaa tttgctgatg cagctgcctc
      781 ccatcacagc ctggcgctcg aatggcggct tgatgatcag cgataacctg agcagccagg
      841 ctgtgcggcg gttctatgag ctcaccagcc agaccttcga tgccaggcga gtggccctga
      901 acgcttttct ggcaggtaat gacctgctct atgtggctga tttcacatcc agcgagctgc
      961 cggaatccat ggaagctacg atccagaccc tgcaattttt cgccgataaa taccgcgaag
     1021 atccggcttt tgcacaacgc gtcgacgagt cggtactgga catcctgacc ctgaaattga
     1081 agctgtacgg cgatttcacc ctgggtttga ccttaccccg tgtagaagac ctggcaaaaa
     1141 tcggaagaag cagttcagtc gtttttgatg tcgcccgcga atccgccaca ttgatcagcc
     1201 cctccgccca ggagctggac gatttgctcc ccgatgcccc caaccgcaac gaccggatgg
     1261 tttttatcac cgatagtcgc ccggcgaagc agtgtactgg ctgtacaacc tccgaaacac
     1321 tgccggtaga tgctctcgag gaagtgatcg tccgccgcta tggcacagcc ggaggcgggc
     1381 agattacccc tgcttacctg acatcttatt cgattaacga tctgcaaacc atgctggcag
     1441 gcttgccagg aaccacccag atggacgtag acctcagtcg cgctcaatgg attgtgtttg
     1501 ccctgcagga caattctccc caataccttg cctaccagac cttaagccag tttctctccg
     1561 aacgacctga tttattccag caaaaacggc tcattgtttt cgctttcaac gcaccgttct
     1621 acctggacgc gacaaacatt accaagctta ctgcctatta tgctttatac agcaaagccg
     1681 cggcatttat cgacatggcc agttatctat tgttcaggga gattccggcc accggcgccc
     1741 tgcccattac ggtgccaggg gtctatgact taaatgcagc cttattcccg gatgcatctc
     1801 aaatcttgca agtgatggtg gattcaccca ctggcagtcc tacccagggc acaggcactc
     1861 ctgacccggc ccctgcgctt gaattcgcag ttggtgaaac cttatctctg cgcacgggtg
     1921 tggtccgcga taacaacggc aatcctgtac ccgacggcac gcctgtcgat ttcatcttaa
     1981 accagggcgg tgaaacccgc cagccggctt tcaccgtgaa tgggattgcc cgcctggcct
     2041 ttattatcaa ccagccgggt gtgctggagg tgcgcgtcga aaccgagacc gccagacagt
     2101 caaacatcct gcat
//
LOCUS       DATMXK010000107         4666 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_16125, whole genome shotgun sequence.
ACCESSION   DATMXK010000107 DATMXK010000000
VERSION     DATMXK010000107.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4666)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4666)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4666
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16125"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            154..336
                     /locus_tag="VN363_01935"
     CDS             154..336
                     /locus_tag="VN363_01935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83293.1"
                     /translation="MDLSTRHRYPGMLKIPEWSSEQEEFTMARLLSTDSTPANQAKVL
                     ASWAAHRVYSPSTAAT"
     gene            complement(309..638)
                     /locus_tag="VN363_01940"
     CDS             complement(309..638)
                     /locus_tag="VN363_01940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83294.1"
                     /translation="MLAGLLDPPVPLLQIEPGQHPDEDRSPQPEISQHFGKGHPIVAQ
                     ELTPRSLGMNLCYPRLSDQQRKVDRCGCSGLAVKQVGQDFGDRAFIRFGSLASLLFGQ
                     VAAVLGE"
     gene            820..1098
                     /locus_tag="VN363_01945"
     CDS             820..1098
                     /locus_tag="VN363_01945"
                     /inference="COORDINATES: protein motif:HMM:NF024769.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YdeI/OmpD-associated family protein"
                     /protein_id="HWQ83295.1"
                     /translation="MTEAGMVRVMEAQAYGEWKAALQRQDGLTMPDDLKAALAENSLA
                     QANFDRFPPSYKKRLIYWITSARTDKTRQSRIQKTVAIAAVNQRSGRM"
     gene            complement(1129..1893)
                     /locus_tag="VN363_01950"
     CDS             complement(1129..1893)
                     /locus_tag="VN363_01950"
                     /inference="COORDINATES: protein motif:HMM:NF015949.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF364 domain-containing protein"
                     /protein_id="HWQ83296.1"
                     /translation="MSLIQELLQVVPDGTVEEVHIGLHWTAVSVQVDGTLRCGLASTL
                     HGPHQHSGPDVPQAGRLTELTARELGALAELSDQPTLCSLGVAALNALLSPGIQETPD
                     INAEEMIARLGAGKRVALVGHFPFVEHLRERVGELVVLELNPRPGDLPAEAAPEVLAG
                     AEVAAITGMTVQNHTLESLLRLAAPGAVVLVLGPSTPMHPCLFEHGIGVLSGSLVTNI
                     PNVMRLVSQGANFRQIHHHGVSLVNLVRPDLAAILS"
     gene            complement(1890..2120)
                     /locus_tag="VN363_01955"
     CDS             complement(1890..2120)
                     /locus_tag="VN363_01955"
                     /inference="COORDINATES: protein motif:HMM:NF021552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2277 domain-containing protein"
                     /protein_id="HWQ83297.1"
                     /translation="MCRSIQTLRRVDYTPSQTEIEAAALQYVRKISGYRKPSRANQAA
                     FERAVEQIAAATQALLDGLQSNEVVLRRDTTH"
     gene            complement(2148..3032)
                     /locus_tag="VN363_01960"
     CDS             complement(2148..3032)
                     /locus_tag="VN363_01960"
                     /inference="COORDINATES: protein motif:HMM:NF013961.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglutaminase domain-containing protein"
                     /protein_id="HWQ83298.1"
                     /translation="MTQPSPESLAYYTQPGLFTQIDDYAHLFKQFPVGIPERVARVQG
                     LLLNVLWAESYGVTPGEQREDELNLRTVPEKLQRILELDGQALTVTRTAENRLLGNSR
                     DYATLLVAILRLGGLPARARCGFATFFRTGRYEDYWVVEYWDKDQQRWVMVASQIDAY
                     QRSGMGIEWDQFDMPGGLFVSGGRSWLACRMGEADPDDFGVFEFKGLDFVRGNLLRDV
                     LALNKVEPLPWDVWGYLETPVAQLKEHELEGFDQLAALTLDPDANFDTLREVYESDPL
                     VHHPSDWEPETDEADKPA"
     gene            complement(3063..>4666)
                     /locus_tag="VN363_01965"
     CDS             complement(3063..>4666)
                     /locus_tag="VN363_01965"
                     /EC_number="6.1.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0002161 - aminoacyl-tRNA editing activity
                     [Evidence IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0004827 - proline-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA];
                     GO_process: GO:0006433 - prolyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="proline--tRNA ligase"
                     /protein_id="HWQ83299.1"
                     /translation="EMNAIGGQEVSMPVVQPADIWKETKRWYQIDAEMGRFKDRNGRD
                     MVLAMTHEEAVGDLVRREIRSYRQLPALIYHLQTKFRDDPRPRAGLIRVREFTMKDSY
                     SLDADWEGLDRQYRAHYQAYFNIFNRCGLPAIAVKSDVGMMGGKLAHEYMYLTPHGED
                     TLLLCDACGYSANRQIAHFHKPAPIPGEALPVEKVATPNCKTIADVAGFLGVPTSQTA
                     KAVFMIAAIPEGDKTIQRFVFAVIRGDMEVNETKLSNAVKAKELRPATEEEIRAIGAE
                     PGYASPVGLKDALVVVDDLIPMSPNLVAGANEAGYHLKNVNYGRDFSAQIVTDLAAAQ
                     EGDACPNCVAPLRTVRGVEVGNIFKLGTRYSDAMGCTFLDKDGQIKPVIMGSYGIGSG
                     RLLACIAEEHHDDNGLIWPVSVAPYPVHLVVLPGKGEGEQVTAEADQLYADLQAAGIE
                     VLFDDRQESPGVKFMDADLLGMPIRLTVSERAHKAGGVEFKRRDQAEKRVVPLAEVLE
                     AVQAEIAALQAGLDATLVEATFEGE"
BASE COUNT          923 a         1475 c         1342 g          926 t
ORIGIN      
        1 ccccacgccg cgacccacag ccaccgaagt gccgtatcca ccgccaccgc catatccata
       61 atctctcgaa cagccgtaaa tcaatcaaaa aaacgccagg caacctgaca tttcaacaac
      121 taaaagttgc tcggcagccg aaatctggct aaaatggatc tttccacacg gcaccgctat
      181 ccaggcatgc tgaaaatccc ggaatggtca tctgagcaag aggaatttac catggccagg
      241 ctgctttcga ccgactcaac ccccgccaac caggccaaag tcttagcctc ctgggcggcg
      301 caccgggttt actcgcccag cactgccgcg acctgaccga agagcaggct cgccaggctc
      361 ccaaaccgga tgaacgctcg atcgccgaaa tcctggccca cctgcttaac agcgaggccc
      421 gaacatccgc agcgatctac cttgcgctgc tggtcgctga gccgcggata gcagaggttc
      481 atcccgagcg agcgtggggt aagctcctgc gctacgatcg ggtgcccttt cccgaaatgc
      541 tggcttattt ccggctgcgg cgaacgatcc tcatccgggt gttggccggg ctcgatctgg
      601 agcagtggaa ccggcggatc caggagcccg gcaagcaacg ccaaggatcc gtttactggc
      661 gggccaggtc gttggccctc cccgagcagg agcacttggc ggaaattgag aaccggttga
      721 tgccgatact taaaatttgc ggttttcgcc cgcaaaccac gcagtatctg gtcagtttcc
      781 aaccaggagc gagtcacaaa gctgctggcc cagggcagga tgaccgaagc cggaatggta
      841 agggttatgg aagcccaggc ttatggtgaa tggaaagccg ccctccagcg ccaggacgga
      901 ttaactatgc cagatgacct gaaagcagcc ctggctgaga acagcctggc gcaggctaat
      961 tttgaccggt tccccccatc ctataaaaaa aggctgatct actggatcac aagcgccagg
     1021 acagacaaga ctcgccagag ccggatccaa aaaaccgttg caatcgccgc cgtcaaccag
     1081 cgctcggggc ggatgtgatt gcaggggcgt gtctttcagg ttccgttttc agctcaaaat
     1141 ggcagctaaa tctgggcgga ccagattaac caggctcacc ccgtggtggt ggatctggcg
     1201 gaaatttgcc ccctgagaga ccaggcgcat cacattcgga atattggtaa ccagcgagcc
     1261 ggacagcacc ccgatcccat gttcaaacag gcacgggtgc atgggtgtgc tgggtcccag
     1321 gaccaggacc acggctccag gggcagccag cctgagcagg ctttccaggg tgtgattctg
     1381 gacagtcata ccggtgatgg cggcgacttc cgctccggcc agcacttcag gggcggcttc
     1441 cgccggcaaa tcgccaggcc ggggattcag ctccagcacc accagctcgc cgacgcgctc
     1501 gcgcaggtgc tcaacaaaag gaaaatgccc cacgagagcg acgcgtttcc cggcacccag
     1561 gcgagcgatc atctcctcgg catttatatc gggggtttcc tggatacctg gtgatagcag
     1621 ggcgttgagc gctgctacac ccagggagca cagagtcggc tggtcggaga gttccgccag
     1681 ggcacccagc tcccgggcag tcagctcggt caatcttcca gcctggggca catccggccc
     1741 gctgtgctgg tgtggtccgt gcagggtaga tgccagcccg cagcgcagcg tgccgtcaac
     1801 ctgcacgctc actgccgtcc aatgcaggcc gatatgcact tcctccaccg tcccgtcggg
     1861 gaccacttga agcagctcct ggatcaggct caatgggtgg tgtcccggcg gagtacgacc
     1921 tcgttggact gcaggccgtc cagcagggcc tgggtggccg cggcgatctg ctccacggcg
     1981 cgctcaaagg cagcctggtt ggcccgcgag ggtttgcggt agccgctgat cttgcgcaca
     2041 tactgcaggg ctgccgcctc gatctcagtc tggctgggcg tgtaatcaac ccggcgcagg
     2101 gtttggatac tccggcacat atccagctcc tttcatttca ttccggctca agccggtttg
     2161 tctgcttcgt ccgtctccgg ctcccagtcg ctcggatgat gcaccaacgg gtcgctctca
     2221 tacacctcgc gcagggtgtc aaaattcgca tccgggtcca gcgtgagggc agctaactgg
     2281 tcgaagcctt ccagctcatg ttcttttagc tgtgcaacgg gcgtttcgag gtagccccag
     2341 acatcccagg gtagcggctc gactttattc aatgccagca cgtctcgcag caggttgccg
     2401 cgcacaaaat caagcccttt aaattcgaaa acgccaaaat catccgggtc ggcttccccc
     2461 atccggcagg ccagccacga tcgcccaccg ctgacgaata aaccacccgg catatcaaat
     2521 tgatcccact cgattcccat tccactgcgc tggtaagcgt caatctggct ggcaaccatc
     2581 acccaacgct gctggtcctt atcccagtat tccaccaccc agtaatcttc ataccgaccg
     2641 gtcctgaaga aggtggcgaa tccgcagcgt gcccgcgccg gtaaacctcc caggcggagg
     2701 atggcgacca gcagggtggc atagtcccgg ctgttgccca ggaggcgatt ttcggctgtc
     2761 cgggtgacgg tcagcgcctg cccgtccagc tctaagatgc gctgcagctt ttcggggaca
     2821 gtgcgcagat tcagctcatc ctcgcgctgt tcgcccgggg ttacgccata gctttccgcc
     2881 cagaggacgt tcagcagcaa gccctgtact ctggcgacgc gctcagggat acccacaggg
     2941 aactgcttaa acaggtgcgc ataatcgtcg atctgagtga acagccccgg ttgggtgtag
     3001 taagccagag actctgggga aggttgggtc attttgttcg gctccttgat gtcggttatc
     3061 ggttattcgc cttcaaaagt ggcttcgacc agggtcgcat ccagcccggc ttgcagggcg
     3121 gcaatttccg cctggacggc ttccaggacc tctgccagcg ggacgacccg cttttcagcc
     3181 tggtcgcggc gcttgaattc aaccccaccg gctttgtgag cccgctcgct gactgtcagg
     3241 cggatgggca tgcccagcag gtcggcatcc atgaatttta cacccgggct ttcctgccgg
     3301 tcgtcaaaca gcacctcgat ccccgcagct tgcaggtctg cgtaaagctg gtcggcttca
     3361 gcagttacct gctccccttc gcctttaccg gggaggacca ccaggtggac cggatacggg
     3421 gcgacactga ccggccagat cagcccgtta tcgtcgtgat gttcctcagc aatgcaggcc
     3481 agcaggcgcc cggagccaat cccatatgac cccatgatta ccggcttgat ctgcccatcc
     3541 ttatccagga aggtgcagcc catggcatcc gagtagcgcg tgcccagctt gaagatattg
     3601 cccacctcca cgccgcgcac cgtacgcagc ggcgcaacgc agttcgggca ggcgtcgcct
     3661 tcctgggcgg ctgccaggtc ggtgacgatc tgagcgctga aatcccgccc ataattgaca
     3721 ttcttcagat ggtagccagc ttcgttggct cccgccacca ggttcggtga cattgggatc
     3781 agatcatcca ccaccaccag ggcgtccttc aaacccaccg gcgaggcata gccaggctcg
     3841 gcgccgatgg cgcgaatctc ttcttcggtt gccgggcgca gctctttggc cttcacggca
     3901 ttgctcagct tggtctcatt gacttccata tcgccgcgaa tgacggcaaa tacaaagcgc
     3961 tggatggtct tatcaccctc cgggatagcc gcaatcatga aaacggcttt ggctgtctgt
     4021 gaggtgggta caccgagaaa tccggccaca tccgcgatgg tcttgcagtt gggcgtggcg
     4081 actttctcca ctggcagcgc ctcccccggg atgggagcag gcttgtggaa gtgggcgatc
     4141 tggcggttgg ctgaataccc gcaggcatcg cacagcagca gggtatcctc accgtggggc
     4201 gtcaggtaca tatattcgtg ggccagcttg ccgcccatca tgccgacatc cgatttgact
     4261 gcgattgccg gcagtccaca gcggttgaag atattgaaat acgcctggta gtgggcgcgg
     4321 tactgcctgt ccagcccttc ccagtcggcg tccaggctgt agctgtcctt catggtaaat
     4381 tcacgcaccc ggatcaggcc ggcccgcggg cgcgggtcat ccctgaattt ggtctgcagg
     4441 tggtagatca aagccgggag ctggcggtaa gagcggatct cgcgccgaac caggtcgccg
     4501 acggcttctt catgggtcat cgccaggacc atatcccgcc cgttgcgatc cttgaagcgg
     4561 cccatctcgg catcgatctg gtaccagcgc ttggtctctt tccagatgtc cgctggttgg
     4621 actaccggca tgctgacttc ctgcccgccg atggcgttca tctcct
//
LOCUS       DATMXK010000108         2960 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_49215, whole genome shotgun sequence.
ACCESSION   DATMXK010000108 DATMXK010000000
VERSION     DATMXK010000108.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2960)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2960)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2960
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49215"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..586)
                     /gene="truB"
                     /locus_tag="VN363_01970"
     CDS             complement(<1..586)
                     /gene="truB"
                     /locus_tag="VN363_01970"
                     /EC_number="5.4.99.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00431.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004730 - pseudouridylate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(55) synthase TruB"
                     /protein_id="HWQ83300.1"
                     /translation="MENDNSNLDLVSGVLVVDKPIGLTSHDVVQIIRRGTGIRRAGHT
                     GTLDPRASGVLVILIGPAVRLSEFVSASDKRYQATIRLGSTTDTYDGDGQITSTAEVG
                     DITEDNFDEVLQTFVGEMEQVPPPYSAVKVKGRKAYNAARLGEEIELEPRTVQVYSLE
                     ILEWAPPESVVDVYCSSGTYVRSLANDLGKALGTG"
     gene            complement(599..1570)
                     /locus_tag="VN363_01975"
     CDS             complement(599..1570)
                     /locus_tag="VN363_01975"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004527 - exonuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional oligoribonuclease/PAP phosphatase
                     NrnA"
                     /protein_id="HWQ83301.1"
                     /translation="MNPPANLDRILSETIEQADQILVIAHVRPDGDAIGSLLGLGLAL
                     REKGKPVELVVEDGLPATFKNLPGWELVRRKPSAPADLVITVDCSDLARLGSSLAPGS
                     QVDINIDHHITNQNFGRFNLVDTRAVATAEILARHLPEWGYPISPEIAAALLTGLITD
                     SLGFRTSNMTPEALRIAAGLMEAGANLPELYRHALISQTFQAVKFWGSGFSRMQRMDR
                     IVWTSITQTDRLKAQYPGRDDADMINVLSAIDDSDVVILFNEQGNGHIKVSWRSQPGI
                     DITPLALQYGGGGHPAASGADVEGSLAEVEAKVLQSTRNFLRSIGNG"
     gene            complement(1554..1961)
                     /gene="rbfA"
                     /locus_tag="VN363_01980"
     CDS             complement(1554..1961)
                     /gene="rbfA"
                     /locus_tag="VN363_01980"
                     /inference="COORDINATES: protein motif:HMM:TIGR00082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosome-binding factor RbfA"
                     /protein_id="HWQ83302.1"
                     /translation="MVSKLRLQRINERIQMELSDMLVRDISDPRLLGISITGVRVDRE
                     LAFAEIYFSALEGSKRAAEILEGLEHAKGYLRHELAVRIDLRTFPHLRFHWDPTFEKA
                     DSIEKLFSALQSEKVEKQEPAHEPDQDEDEPAS"
     gene            complement(1986..>2960)
                     /locus_tag="VN363_01985"
     CDS             complement(1986..>2960)
                     /locus_tag="VN363_01985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558657.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="translation initiation factor IF-2"
                     /protein_id="HWQ83303.1"
                     /translation="VIGAMIEAERDRAKGVLATMLIQNGTLQVGDTVVVGTTLGKLRA
                     MFDFRGKQIRKAGPSTPVSVMGLNDVPQAGDLFEVVDNEKVGRTIVQQRKQQADEAAA
                     SKQTTATLEQLFERYQAGELRELRLILKADVQGSLEPIVSEVNELGKGDIKVNILHAE
                     TGNIGENDIMLASASDAVVIGFNVQADAAARRLAEAEGVSIRLYDIIYRLTEDLEKAL
                     KGMLEPEERETVIGYARVRAVFNISKLGNIAGCIVTSGEIRRNARVRVKRGEDILYEG
                     NISSLKHLQDDVREVRTGFECGIALKNFTDITEGDIIEAYIVEKVAVA"
BASE COUNT          683 a          837 c          689 g          751 t
ORIGIN      
        1 cccctgtacc cagggctttg cccaggtcgt ttgccagcga gcggacatac gttccagacg
       61 agcagtagac atctacgact gactccggtg gtgcccattc gagaatttcc aggctgtaaa
      121 cctgcaccgt gcgtggttct aactcgatct cctctcccag gcgcgctgca ttatacgctt
      181 ttcgaccttt tacttttaca gccgaataag gggggggaac ctgttccatt tcaccaacaa
      241 aggtctgaag gacctcgtca aaattatctt cggtaatgtc gccgacttcg gcagtactgg
      301 taatttgacc atcgccatcg taagtatccg tggtgcttcc caggcggatc gtagcctgat
      361 agcgtttatc cgatgcgctc acgaactcgc ttaagcgaac tgctgggccg atcaagatga
      421 ccagcacgcc agatgcgcgt ggatcaagag tcccggtatg tccggcgcgc cggatgcctg
      481 tacctctccg gatgatttgc acaacatcat gcgaagtcaa accaattggt ttatcgacga
      541 ccaatactcc cgaaacgaga tctaagttgc tattgtcatt ttccattgtg cagctcttct
      601 agccgttccc tattgatctc aagaaatttc gtgttgattg taacactttc gcttcaacct
      661 ctgccaggga gccttcaaca tccgcgccag atgctgccgg atgaccaccc ccaccatact
      721 gcagcgccag gggcgtgata tcaatgcccg gctgagaacg ccagcttacc ttgatatgtc
      781 catttccctg ttcattaaac aggatgacca catcgctgtc atcgatcgca gataaaacgt
      841 tgatcatatc tgcatcatcc cgcccaggat attgggcttt aagcctgtcc gtctgggtaa
      901 tgctggtcca cacgatgcga tccattcgct gcatacgaga aaaaccgctg ccccagaact
      961 ttactgcctg aaaagtctgg ctgatcaggg catgtctgta aagctcaggt agattcgcac
     1021 cggcttccat taaacccgct gcaatccgca aggcttcggg agtcatgtta ctggtccgga
     1081 atcccaggct atcggtgatc agaccggtca acagcgccgc ggcgatttca ggggaaattg
     1141 gatatcccca ttcgggcagg tgcctggcta aaatttcagc cgtggcaact gccctggtat
     1201 ccaccagatt aaagcgccca aaattctgat tggtaatgtg gtggtcgata tttatatcca
     1261 cctgagaccc tggcgccagg ctgctgccaa ggcgcgccag atcagagcaa tcaactgtta
     1321 ttaccaggtc agctggagca gaaggttttc tgcggaccag ttcccagcct ggcaggttct
     1381 tgaaggtggc cggtaagcca tcttcaacca ccagttctac cggcttccct ttttctcgta
     1441 aagccaatcc caatcccagc agtgatccga tcgcatcacc atccgggcgg acgtgggcga
     1501 taaccaggat ttgatctgcc tgttcgattg tctcgcttag aatgcgatcc aaattagctg
     1561 gcgggttcat cctcatcctg gtcgggttcg tgagccggtt cctgcttttc tactttttcg
     1621 gattggagtg ctgagaaaag cttctcgatc gaatcggctt tttcaaatgt tggatcccag
     1681 tggaaacgca gatgagggaa tgtccgcagg tcaattcgca ccgctaattc gtggcgtaag
     1741 taacctttgg catgctccag accttccagg atctcggctg ctcgttttga gccttccagg
     1801 gcagaaaaat agatttctgc aaaagccagt tcacgatcca cgcgcacacc cgtgatcgag
     1861 atacccaata accgtgggtc ggagatatct cttaccagca tatcggaaag ctccatctgg
     1921 atacgctcat taatgcgctg gaggcgtagt ttagaaacca actctctctt ccttacccct
     1981 gtggtttaag ccacagctac tttctcaacg atatacgctt cgataatatc gccttcagtg
     2041 atatcggtga aattcttcaa agcgatacca cactcaaagc cggtgcggac ctcacggaca
     2101 tcgtcttgta agtgcttcag cgatgagata ttcccttcat atagaatatc ctcgccgcgc
     2161 ttaacgcgca ctcgcgcatt gcggcggatc tcaccactgg tcacaatgca tccggcaata
     2221 ttacccagtt ttgagatatt gaaaactgcc cgtactcgag cataaccaat aacagtttct
     2281 ctttcctctg gctcgagcat acctttgagc gctttttcca ggtcttctgt caaacggtag
     2341 ataatgtcat agagcctgat cgaaacacct tctgcttcag caaggcgtct tgcggctgca
     2401 tcagcctgga cattgaagcc gatcaccaca gcatctgaag ccgaagccaa cataatatca
     2461 ttctcaccga tatttccagt ttcagcatgc agaatattga ctttgatatc gcctttgccc
     2521 agctcattga cttctgagac gatgggttcc agggagccct gaacatcggc tttcaagatc
     2581 aaacgcagct cacgtaattc acccgcctga taacgctcaa acaactgttc cagggtggca
     2641 gtagtttgct tgctggcagc tgcttcatca gcctgttgtt ttcgctgctg gacaatcgtt
     2701 cggccaacct tttcattatc aaccacttca aacaggtcgc ctgcctgggg tacatcattc
     2761 aaccccataa ccgataccgg ggtcgaaggg ccagccttgc ggatttgctt gccgcgaaaa
     2821 tcgaacatcg ctcgcagctt acctaaagtc gtccccacga caacggtatc tccaacctgc
     2881 agggtgccgt tttggattaa catggttgca aggacgcctt tggcccggtc gcgctcggcc
     2941 tcgatcatcg ccccgattac
//
LOCUS       DATMXK010000109         2833 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_72034, whole genome shotgun sequence.
ACCESSION   DATMXK010000109 DATMXK010000000
VERSION     DATMXK010000109.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2833)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2833)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2833
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72034"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1212
                     /locus_tag="VN363_01990"
     CDS             <1..1212
                     /locus_tag="VN363_01990"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Smr/MutS family protein"
                     /protein_id="HWQ83304.1"
                     /translation="VTNIIRILEKANPRSLVILDELGAGTDPQEGAALARAILTHVLG
                     RSITTLVTTHHPELKVFAHSTPGVVNASVEFDLETLRPTYHLTVGLPGRSNALAIAQR
                     LGMPAEIISEARSEINPDDLRAEDLLDEIHRQRDLARQARTAADKARREAETLRAELE
                     QRLDGIDDERRKVIEQARSQAETELRDVQEELRQARRTLARARQPVEALQAVEAQVEK
                     LEELVAKPVERKAPDIALPHRRAVRLGDKVRVRSLNTQGVLTSLGEEEAEVQVGMLRI
                     RTRLAELELSGGSPVIEQPSTTPSPTPRPTSSDSPPVPAASYLLASPGMEIDLRGQRA
                     DEALDAIERYLDAAYLSGLPFVRIIHGKGTGRLREVVRQMLHGHPHVRSYEGGSDKEG
                     GDGVTVVKLAQ"
     gene            complement(1231..2334)
                     /locus_tag="VN363_01995"
     CDS             complement(1231..2334)
                     /locus_tag="VN363_01995"
                     /inference="COORDINATES: protein motif:HMM:NF014164.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase"
                     /protein_id="HWQ83305.1"
                     /translation="MNDPRLENLAKILVDYSIAVKPGDWVVVRGSSLAFPLLEAVVRN
                     IYQSGGHPTTLIENDALEEIHLRYASDAQLEWISPFETLAFQKMDALITVRAPGNTRL
                     LSGIDPQKTRLQTLARKDLHQTYMDRSAEGSLRWVSTQYPCPAYAQDADMSLSDYEDF
                     VYAATFADQPDPVACWHNVHAEQQRLVEWLKGKDRVSVRGPNCDLSLSIAGRKFSNSD
                     GHRNMPSGEIFTGPVEDSVDGWIRFTYPAIYRGREVEGVELKFKAGKVVNARASKNEA
                     YLLALLDSDPGARYLGEFAIGTNYGIQRFTRSILYDEKIGGSIHLAVGAGYPETGSLN
                     KSAIHWDFICDMRDGSEILVDGELFYRNGKFML"
     gene            2449..>2833
                     /locus_tag="VN363_02000"
     CDS             2449..>2833
                     /locus_tag="VN363_02000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83306.1"
                     /translation="MKPQSAYHLLVLLLIIILTGCQTLVPLPPTPTATITPTETPTPS
                     PSPTATASPTLTSTPTPTETPTATPSPTPLLLAADSAPLPETLSPVTVENAGQVSALA
                     EWQTDPVVDLVWLPDGSQLVAAAANE"
BASE COUNT          592 a          837 c          828 g          576 t
ORIGIN      
        1 gtgaccaaca tcatccgcat ccttgagaaa gccaacccgc gctccctggt cattctggat
       61 gagttgggcg ccggcaccga cccgcaagag ggtgccgccc tggcgcgtgc gatcctgacc
      121 catgtcctgg ggaggagtat caccacactg gtaaccaccc accacccgga gctgaaggta
      181 tttgcccact ccacgccggg cgtggttaac gccagtgtag aatttgacct ggaaaccttg
      241 cgcccgacct accatctcac cgttggatta ccaggccgct ccaatgccct ggcaatcgcc
      301 cagcggttgg ggatgccggc agagatcatc agcgaggcgc gcagcgagat caacccggac
      361 gatttgcggg ctgaggatct gctcgacgaa attcatcgcc agcgtgacct ggcacgccag
      421 gcacggactg cggccgataa agctcgccga gaggcggaaa cgctgcgggc tgagctggaa
      481 cagcgcctgg atgggatcga tgacgaacgc cggaaggtga tcgagcaagc ccgttcccag
      541 gctgaaaccg agctgcggga tgtacaggaa gaattacgcc aggcgcgacg caccctggcg
      601 cgtgctcgcc agccggtaga agcgctccaa gctgtcgaag cgcaggttga aaaactggaa
      661 gagctggtgg caaaaccggt ggagcgcaaa gccccggata tcgccctgcc gcaccgccgg
      721 gctgtccgcc tgggagataa ggtgcgggtg cgttcgctga acacgcaggg tgtactcact
      781 tcactcgggg aagaagaggc agaggtccag gtaggcatgc tgcgcatacg cacccgcctg
      841 gcagaactgg agctgtctgg gggcagcccg gtgatcgaac agccctcaac gacgccgtct
      901 ccaaccccca gaccaaccag cagcgattct ccgcctgtac cggcggcctc gtacctgctg
      961 gcttcgccgg gaatggaaat agatctgcgc ggccagcgcg cagatgaagc gttggatgca
     1021 attgagcgct acctggacgc ggcttacctg tccggtttgc catttgtaag gattatccat
     1081 ggaaaaggta ccggcaggct gcgggaggtg gtgcgccaga tgctccatgg acacccgcat
     1141 gtgcgctcat atgaaggcgg cagtgataaa gaaggcgggg atggggttac ggtcgtaaaa
     1201 ttagctcaat aaccactttc gcgtgctgcc tcagagcatg aattttccgt tgcggtagaa
     1261 cagctcccca tcgaccagga tctcacttcc atcgcgcata tcgcagataa aatcccaatg
     1321 gattgcggat ttattcaagc tgccggtttc ggggtagcca gcgccaacgg ccaggtgaat
     1381 acttccgccg atcttttcat cgtacaggat gctgcgggta aagcgctgga tgccataatt
     1441 cgttccgatg gcgaactcgc ccaaataccg cgcccctgga tcgctgtcca ataaagccaa
     1501 taaataagct tcgttcttgc tggctctggc attgaccacc ttgccagctt tgaatttcag
     1561 ctcgacgcct tcgacctcac gccctctgta tatcgccggg taagtaaagc ggatccagcc
     1621 gtccactgaa tcttccactg gtccggtgaa gatttcgccg ctgggcatgt tgcggtgccc
     1681 atcgctgttg ctgaacttgc gaccggcaat ggagaggctc aggtcgcagt tgggtccgcg
     1741 cacgcttacc cggtcttttc ctttgagcca ctccaccagg cgctgctgtt cagcgtggac
     1801 attatgccag caggctaccg ggtcaggctg gtcggcgaaa gtggcggcat agacgaagtc
     1861 ctcataatcg ctgaggctca tgtctgcatc ctgggcgtac gccgggcagg gatactgggt
     1921 gctcacccag cgcaggctgc cttctgcaga gcgatccata taagtttggt gcaagtcctt
     1981 gcgggcaaga gtttgaaggc gggttttctg cgggtcgatc ccgctcaaga ggcgggtatt
     2041 cccgggggcg cgcacggtaa tcaatgcatc catcttttgg aaggccaggg tctcgaaggg
     2101 agaaatccat tccagctggg catcgctggc ataacgcagg tggatctctt ccagggcgtc
     2161 attttcgatc agggtggttg gatggcctcc ggactggtag atatttctga caaccgcttc
     2221 caataagggg aacgccaggc tgctgccgcg cacaaccacc caatccccgg gtttgacggc
     2281 gatggagtaa tcgaccagga ttttagcgag attttcaaga cgtggatcgt tcattaaacc
     2341 ctcctgtaat gtgaattatg ctcgatttgt gattatactc tcctgcaggg tcttgaacct
     2401 cggggtagtg ctacaattag gtatcctgtt ggagaggctg gctgatcgat gaaaccccaa
     2461 tctgcttatc atctcctggt attgctgctt atcattattc tgaccggctg ccagaccctg
     2521 gtgccactgc cgcctacacc gacagcaacc attaccccaa ctgagactcc gacccccagc
     2581 ccttcgccaa cggcaaccgc cagccctact cttaccagca ccccgacgcc tacagaaaca
     2641 cccaccgcaa ccccttctcc aacacccctg cttctggctg cggattctgc gccgctcccg
     2701 gaaactcttt cgccggtgac cgtggagaat gccggtcagg tttcggcgct ggcggagtgg
     2761 cagaccgatc cggtggtcga tctggtctgg ctgccggatg gcagtcagct ggtggcagcc
     2821 gcagcaaatg aga
//
LOCUS       DATMXK010000110         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_152610, whole genome shotgun sequence.
ACCESSION   DATMXK010000110 DATMXK010000000
VERSION     DATMXK010000110.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152610"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2827)
                     /gene="nifJ"
                     /locus_tag="VN363_02005"
     CDS             complement(<1..>2827)
                     /gene="nifJ"
                     /locus_tag="VN363_02005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005506 - iron ion binding [Evidence IEA];
                     GO_function: GO:0016903 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="pyruvate:ferredoxin (flavodoxin) oxidoreductase"
                     /protein_id="HWQ83307.1"
                     /translation="ASNSGQEAQDMALIAHAATLESRVPFLHFFDGFRTSSEVTKIER
                     LGIEDLRAMIDDQLVQAHRARGLTPDRPVLRGTAQNPDVYFQARETVNPYYLACPEIV
                     QNTMDRFAALVGRQYSLFEYTGDPQAERVIIMIGSGAETAGETAKYMNEHGEKVGVLA
                     VHLYRPFSVKHFLQALPATVKSIAVLDRTKEPGAAGEPLYQDVVTALVEGWKGGFPKV
                     IGGRYGLSSKEFTPAMVKAVYDELLKPEPKNHFTVGIIDDVTLTSLDYDPSFFIESPE
                     TVRCMFWGLGSDGTVGANKNSIKIIGEETDNYAQGYFVYDSKKSGSVTISYLRFGPKP
                     IRAPYLIDQAQFVACHQFSFLERYDVLKDAQPGGVFLLNSIFGPDKVWENLPVETQQT
                     IIDKHLRFYVIDAYEVAEKTGMGGRINTVMQTCFFAISGVLPREQAIAEIKRAIKKTY
                     GKRGEAVVQQNYAAVDSAVENLFEVKVPAEADSPITRRLAVPELAPEFVKEVLGAMIS
                     RQGDELPVSALPVDGTYPTGTTKWEKRNISLEIPVWEPDLCIQCGKCSYVCPHAVIRE
                     KVYDPKYLEAAPEGWNSRDARWKEFPGSKYTLAVAPEDCTGCGLCIEACPAKDKSQVG
                     RKAINMAAQPPIREVEAVKWDYFLSLPEADRTQMSLKTVKNSQLLEPLFEFSGACAGC
                     GETPYLKLLSQLYGDRVVIANATGCSSIYGGNLPTTPWAQNAAGRGPAWSNSLFEDNA
                     EFGLGMRLTLDKRGEYARELLPQFAEKVGGELVSELLQADQSTEAGINAQRERVAILK
                     QRLESITEPAGRELASIADVLVRKSVWIVGGDGWAYDIGYGGLDHVLASGRNVNVLVL
                     DTEVYSNTGGQASKSTPRAAVAKFAAQGKAIPKKDLGMIAMSYGYVYVARVAMGASDQ
                     QTLSAFIEADEYDGPSLIIAYSHCIAHG"
BASE COUNT          657 a          767 c          748 g          655 t
ORIGIN      
        1 gccgtgggca atgcagtggc tgtacgcaat gatcagagac gggccatcgt actcatcagc
       61 ttcaatgaac gcactgagag tctgctggtc actggcgccc atggctacgc gagcgacata
      121 gacataacca taagacatag caatcatacc caggtctttt ttagggattg ccttaccctg
      181 agcggcgaat ttagcaacag ccgcgcgtgg agtggactta gaagcctggc caccggtatt
      241 ggaatacact tcggtatcaa gcaccaggac attgacattg cggcctgaag ccaggacatg
      301 atccagacca ccataaccaa tatcatatgc ccaaccatcg ccgccgacga tccaaacact
      361 tttgcggacc aatacatcgg caatgctggc aagttcgcgc ccggcaggct cagtgatgct
      421 ctccagacgc tgcttgagga tggcaacgcg ttcgcgttgg gcgttgatcc cggcttcggt
      481 gctctggtct gcctggagaa gctcactgac cagctcccct ccaacttttt cggcaaactg
      541 aggcaaaagc tcgcgagcat actcaccgcg tttatccagg gtcaggcgca tgcccagccc
      601 gaactcagca ttatcttcga agagggagtt ggaccaggca ggcccgcgcc cggcagcgtt
      661 ttgcgcccag ggtgttgtag gcaggttacc gccataaatg gatgagcagc ctgtggcatt
      721 tgcgataacc acccgatccc cataaagctg gctgagaagc ttcaggtacg gggtttcgcc
      781 gcatccagcg caagcacccg aaaactcaaa gagaggttcg agcagctgcg aattcttgac
      841 tgtcttgagg ctcatctggg tgcgatccgc ttccggaagg cttaagaaat aatcccattt
      901 gacagcttca acctcgcgga tgggtggctg agcagccatg ttgatggctt tacgacctac
      961 ctggctctta tcctttgccg ggcaggcttc gatgcacaaa ccacagccgg tacaatcttc
     1021 gggggctaca gccagggtat atttcgatcc agggaactct ttccagcgcg catccctcga
     1081 gttccagcct tctggagcag cttccaggta ttttggatca tatactttct cacggattac
     1141 tgcgtgcggg cagacgtacg agcatttacc gcactggatg cacaaatcag gctcccacac
     1201 cggaatctcc aggctgatgt tgcgtttttc ccatttggtc gtaccggtcg ggtaggttcc
     1261 atccaccggc agcgcgctca ccggaagctc gtcaccctgg cgggagatca tggcacctaa
     1321 cacttctttg acaaattcgg gtgccaattc tggcacggca agccggcgag tgattgggct
     1381 gtctgcttca gcaggaactt tcacctcaaa caggttttct acggcgctat cgacggcagc
     1441 ataattctgc tggacaaccg cctcaccccg cttgccatag gttttcttga tggcccgttt
     1501 aatctcggca atcgcctgtt ctcttggaag aacaccgctg attgcgaaga agcaggtctg
     1561 cataacagta ttgatccgtc cgcccatacc ggttttttcg gcgacttcat aggcatcgat
     1621 aacatagaaa cgcaggtgtt tatcgatgat cgtttgctgg gtctcgactg gcaggttctc
     1681 ccagactttg tctggcccga aaatgctgtt caacaagaac acacctccgg gctgggcgtc
     1741 tttcaagaca tcataacgct ctaagaaaga gaactggtgg caggcaacga attgagcctg
     1801 gtcgatcagg tatggtgcgc ggataggctt aggaccaaaa cgcagataag atatcgttac
     1861 cgagcccgac ttttttgagt catagacaaa atagccttga gcgtagttat cggtttcttc
     1921 gccaatgatt ttgatcgagt tcttattagc tcccactgtg ccatctgagc ccaagcccca
     1981 gaacatgcag cggacagtct caggcgattc gatgaagaaa ctgggatcat aatccaggct
     2041 ggtgagggtg acatcatcaa taatgccaac cgtaaaatga tttttgggct caggtttaag
     2101 gagctcatca tagactgctt tgaccatggc cggggtgaat tctttggagg agagaccata
     2161 acgcccaccg atcactttgg ggaagccgcc cttccaacct tcgaccagag cagtcacaac
     2221 atcttggtag aggggctcgc ctgctgcgcc gggttctttg gtgcggtcca gaacagcaat
     2281 cgatttcact gtggcgggca aagcctggag aaagtgttta accgagaatg gtcgatacag
     2341 gtgaacagcc aggacgccta ctttttcgcc gtgctcgttc atatatttag cggtttcacc
     2401 agcggtttcg gcgcccgagc cgatcatgat aatgacacgt tcggcttgcg gatcgccggt
     2461 gtattcaaat aatgaatact gccgcccgac aagagcggcg aagcgatcca ttgtgttttg
     2521 gacaatctca ggacacgcca aataataggg gttgacggtc tcacgtgcct ggaaataaac
     2581 atccgggttt tgggctgtcc ctcgcaaaac tggccggtcg ggagtcagtc cccgcgcccg
     2641 atgagcctgc acaagctgat catcgatcat agcccgcaga tcttcaatcc ccagacgctc
     2701 tattttggtt acctcagagg atgtgcggaa accatcaaag aagtgcagga atggcacgcg
     2761 gctttctagt gtagctgcat gggcaatcaa tgccatgtcc tgggcttctt gaccggaatt
     2821 cgaggcc
//
LOCUS       DATMXK010000111         2359 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_128895, whole genome shotgun sequence.
ACCESSION   DATMXK010000111 DATMXK010000000
VERSION     DATMXK010000111.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2359)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2359)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2359
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128895"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..360
                     /locus_tag="VN363_02010"
     CDS             <1..360
                     /locus_tag="VN363_02010"
                     /inference="COORDINATES: protein motif:HMM:NF014357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HWQ83308.1"
                     /translation="VKNGFGTCPIPPVNPDEDRASALANDCVLYGGRVTYTVRCEDSE
                     LEALIERIPSSSSPSYGIPFYELYQQFGSFYNVDPLIFCPSQVTMNNLTSGRVFRAGQ
                     PHLGVLRQSILGEGRVG"
     gene            488..769
                     /locus_tag="VN363_02015"
     CDS             488..769
                     /locus_tag="VN363_02015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83309.1"
                     /translation="MSDDELENGDENGEENGLPDEFKEQLGQVVDEHFSKKEREDAQD
                     LIRDAFAFLPMELQDLYHDIQLAYRAGKFEEASKLFEQWLGEARDLGLI"
     gene            787..1641
                     /locus_tag="VN363_02020"
     CDS             787..1641
                     /locus_tag="VN363_02020"
                     /inference="COORDINATES: protein motif:HMM:NF020738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NADP-dependent
                     methylenetetrahydromethanopterin/methylenetetrahydrofolate
                     dehydrogenase"
                     /protein_id="HWQ83310.1"
                     /translation="MKKLLLQLDSDKLASSFDTITAYDAGADHVLSFGGVGVEDVRNI
                     IYGAMFTRGGEMLKNSAVFIGGSSVPVGEAMLKAAQEAFFGPVRVSVMADPNGSNTTA
                     AAAVLKIIRATPVAGKKAVIYAGTGPVGVRAAALLVKEGCQVLLTSRKRDRAQEACKS
                     IADRFQLEVTPMEVREESSVIQALEGAHIALACGAAGIELVKKELWAASPTMEVVADV
                     NAVPPTGIGGIKPSDDGKEREGKIAFGALGIGGLKMKIHRAAVARLFEQNDLVLDIEE
                     IYAIGKGL"
     gene            1703..1846
                     /locus_tag="VN363_02025"
     CDS             1703..1846
                     /locus_tag="VN363_02025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83311.1"
                     /translation="MVAECPVCLRDVTIDENAVEGDIVKCPVCKEWFRLVRINGEWKG
                     ERI"
     gene            1859..>2359
                     /locus_tag="VN363_02030"
     CDS             1859..>2359
                     /locus_tag="VN363_02030"
                     /inference="COORDINATES: protein motif:HMM:NF018968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1464 family protein"
                     /protein_id="HWQ83312.1"
                     /translation="MRVVGIDPGTVSFDLCGLEDGRVFLDTTLPSADFATDPKLLLDL
                     LTSSVPLDLVIAPSGYGLPLVPIEEFGDAERFLFTLVDERERGRIPVLGGMGKVIRLL
                     KESGLPVVFMPGVIHLPSVPEHRKANKIDMGTADKLCCLALGIYNQAQRADIPYSETS
                     FILAEVG"
BASE COUNT          559 a          554 c          746 g          500 t
ORIGIN      
        1 gtaaaaaacg gctttggaac ctgtccaata ccaccggtaa atccggatga agaccgggcc
       61 agcgccctgg cgaatgactg cgttctgtat ggcgggcggg tgacttacac cgtgcggtgc
      121 gaggacagcg agcttgaagc attgatcgag cgcatccctt catcgtcttc gccatcctat
      181 gggattccat tctatgagct ttaccagcag tttggcagtt tttacaatgt cgatccgttg
      241 atattctgcc cgtcgcaggt gacgatgaat aatctgacca gcgggcgggt atttcgggca
      301 gggcagcccc acctgggcgt gctccgccaa tccatcctgg gagaaggcag ggtgggttaa
      361 atggttagtg aagataaaag cgaccatgtt cagctctact ggcgcggatc atcgaaccta
      421 tcgagtgcca tcttcagtcg gataggcgcg ttcatcctgt ttgggaaata aaccgagcga
      481 ggcgatcatg agcgacgatg aacttgaaaa tggcgatgaa aatggcgaag aaaatggcct
      541 gccggacgaa ttcaaagagc agctcggcca ggtggtggat gagcactttt ccaagaaaga
      601 acgcgaagat gcccaggatt taatccggga cgcgtttgct tttttgccca tggagctaca
      661 agatctttat cacgatattc agctggccta tcgagctggc aaatttgagg aagcatcaaa
      721 attattcgaa caatggcttg gcgaggcgcg agatttagga ttaatctaaa taaaagaggc
      781 aacctgatga aaaaattact cctccaactg gacagcgata aactggcaag ctcgttcgac
      841 acgatcacag cctacgacgc gggagcagac catgtgctga gtttcggcgg ggtaggagtt
      901 gaggatgtac gcaatatcat ttatggggca atgttcaccc gcggcgggga gatgctcaaa
      961 aactcggcgg tgttcattgg cgggtcgtcg gtgccggtgg gcgaagcgat gctcaaagcc
     1021 gcgcaggagg ctttctttgg gccggtgcga gtatccgtga tggcagaccc gaatggatcg
     1081 aacaccacag ccgcagcggc ggtgctgaag atcatccggg cgactcctgt ggcaggaaaa
     1141 aaagcggtga tttacgccgg aaccggcccg gtgggcgtgc gggctgcggc gctgctggtc
     1201 aaggagggct gccaggtact gctcacttcc cggaagcgcg accgggccca ggaagcttgc
     1261 aaatcgattg ccgaccggtt ccaattggaa gttaccccga tggaagtccg ggaggaatcg
     1321 agcgtgatcc aggcgttgga aggggcacat atcgccctgg catgcggcgc tgccgggatc
     1381 gagctggtga agaaagaatt atgggctgcc agcccgacaa tggaagtggt ggcagacgtg
     1441 aatgcggtgc cacctaccgg aatcggcggg atcaaacctt ctgatgacgg caaagaacga
     1501 gaaggtaaaa ttgcttttgg ggcattgggc atcggtgggc tgaagatgaa gatccaccgc
     1561 gccgctgtcg ccaggttgtt cgaacagaac gacctggtgc tggatatcga agaaatctat
     1621 gcgattggta aagggctcta agccagggat gcgcggcgct aaggctcaca cccagagcgc
     1681 cagagcaaag gagtgacacc tgatggttgc tgaatgcccg gtctgtctgc gggatgtaac
     1741 gatcgatgaa aatgcggttg aaggcgatat cgtgaagtgt ccggtttgca aagaatggtt
     1801 caggctggtg cgaattaatg gcgaatggaa aggcgaacga atttaagagg tgacagtgat
     1861 gcgagttgta ggtatcgatc ccggtacggt tagttttgac ctgtgtggtc tggaggatgg
     1921 gcgggtattt ctggatacga ccctgccatc tgccgatttt gcgacggacc caaaactgct
     1981 gctggatctt ttgaccagct cggtcccttt ggacctggtg attgcgccct ccggatatgg
     2041 gctgccgctg gtacccatcg aagaatttgg cgacgcggag cgctttttgt ttacgctggt
     2101 ggacgagcgc gaacggggaa ggatcccagt gctcggcggg atgggcaagg taatccgcct
     2161 gttaaaggag agtggattac cggtagtatt tatgcccggg gtgatccacc tgccgagcgt
     2221 gcccgagcac cgcaaagcca acaagatcga tatgggtaca gccgataagc tgtgctgcct
     2281 ggcattggga atctacaatc aagcgcagcg ggcagacatt ccctactctg aaacctcctt
     2341 catcctggct gaggtgggg
//
LOCUS       DATMXK010000112         4797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_93931, whole genome shotgun sequence.
ACCESSION   DATMXK010000112 DATMXK010000000
VERSION     DATMXK010000112.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93931"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1317)
                     /locus_tag="VN363_02035"
     CDS             complement(<1..1317)
                     /locus_tag="VN363_02035"
                     /EC_number="3.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:NF014156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-galactosidase"
                     /protein_id="HWQ83313.1"
                     /translation="MLSLETSTLCLTIAPDQAAWSLAYQTVPDFEFTQVHLRLHYIQG
                     GQRRTLLDDWPGCTVSGPGNSPSPLGMSQTVHLVFTPAPGLEMELVFALHESPACLLW
                     KIKLVNQDTVPLTMERIELIADHPAATIHLGEHRQTPPNDLAFFSNGWQSWSYTGAYA
                     PQDRFHHTRLGFLHQPMVLNAGTPRPGQPGHFASDMFAVLGSRSTRRAWLAGFLSQLQ
                     HFGSLEARLDRSPPALRMWANGDNTRLDPGQSMETDWACLFPIQLDDPDPLGAFAHAV
                     AQTYACTGLQHQAISGWCSWYHYYLKVRPADVLDNIQAASTLRHRLPLELIQLDDGFE
                     YAVGDWLSFSSNFPQGVAPLAAQISAQKFTPGLWLAPFIVDPRSELAHTHPDWLLRGR
                     FNRPVNAGFTFWGSFATGLDLTHPEAMDYAARVTRTAAREWGYPFLK"
     gene            complement(1363..1722)
                     /locus_tag="VN363_02040"
     CDS             complement(1363..1722)
                     /locus_tag="VN363_02040"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWQ83314.1"
                     /translation="MQIKSELISPHQAVLYPTGRMDVESSAAVRQAILDLVDSGVRYL
                     VVDLVQVEFMDSSGLSALVSGMKALRRSSGMLTICQANAQIRTALRLTMLDRVFPIYE
                     DIPAALEAAETDQPPAL"
     gene            complement(1773..3827)
                     /locus_tag="VN363_02045"
     CDS             complement(1773..3827)
                     /locus_tag="VN363_02045"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWQ83315.1"
                     /translation="MSEMLAQVGGLWRDLTGHELFFWGDGALLSASNTAPAGLLAALN
                     DTLQHHPAPVETILWQNQTWLIGRLGPSPTQISIASLLTDPAAEAQFERMTGLLDGLA
                     ESQQAAQQLGTQLSASSGHLNFLYNLAQFSFQTDDPAQLYPRLAQSLSQVSEAEDICL
                     AFWEADQPHIYTASGRNPSGLEKLAVYASTAAPLSVLSSADSVPRALLRAIPNLKNLL
                     ILPLPLTGELRGLVALVNHSTGSLNQADQQLLTNAGELISMLITSQFARHNRDADRRL
                     ELELSFASQIQAIFLPTTLPDIPELEFGASLMPAHLISGDFYDVQGMQDGWAIMVGDV
                     AGKGIPAAMLAAMIHATLKSEVHHHTEPAELLQSINHLIYAELDRSETFITSFLAVLQ
                     TRPLQLHYASAGHTTTLLWRSAEQHVLQLPSTGLPLGIYPESVYAEHHLPLKPGDVLV
                     LYSDGITEAENDQGRVFGGQALIDLLLAGHPAPIDKQLQLIFNALDLHRGKLPLRDDV
                     VLLMVRARGTETATLVQPFVFMAEKRSVRTLATQVRQVASSLTFSGPAERSAFLNDLE
                     LAVSEITANIVIHAYHDSPYLGRIQGRVTLFPEKVQVDLIDSGIQFNSPTGLSLLQAE
                     PAFSPSDPPTGGYGLSIARRLLDTCAYTRLPEGRNHWHLEKFISQVTSLPRPDPATGL
                     FH"
     gene            complement(3966..4550)
                     /locus_tag="VN363_02050"
     CDS             complement(3966..4550)
                     /locus_tag="VN363_02050"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013052.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0016279 - protein-lysine
                     N-methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SET domain-containing protein"
                     /protein_id="HWQ83316.1"
                     /translation="MQHPNTELRFVNPQVGYGVFATTFIPAGTIVYARCSLDIRISPS
                     SPLLADPAYRDLILKYAYTDPNGDFIICWDIGKYVNHCCHASAVSTGYGFDIAVRDLQ
                     PGDEITNDYAQFSFDEEMDLFCHYPDCRKKFTPQDFDRLHPDWDAQVKSALQKFAEVP
                     QPLQQYLDPQTASDLQRYLATGEGYRSVRSLRPW"
     gene            complement(4594..>4797)
                     /locus_tag="VN363_02055"
     CDS             complement(4594..>4797)
                     /locus_tag="VN363_02055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83317.1"
                     /translation="ILGARHLEQLQDNLGALDLTLTPEQIFKLDSLSPIQLGFPHDFL
                     SGSEVRSLLFGDTHPLIDNHRPG"
BASE COUNT         1078 a         1246 c         1534 g          939 t
ORIGIN      
        1 ttttaaaaat gggtagcccc attcacgggc ggctgtccgg gtaacccggg cagcgtagtc
       61 catggcttcg gggtgagtca aatccaggcc ggtggcaaag ctgccccaaa aagtaaagcc
      121 ggcgttgaca ggacgattaa agcggccgcg caagagccaa tcggggtggg tgtgggccag
      181 ctcggagcga gggtcgacga taaagggcgc cagccacaga ccgggagtaa acttctgcgc
      241 gctgatctgg gctgccaggg gagccacacc ctgagggaaa ttggaggaga agctgagcca
      301 atctcccacc gcgtattcaa agccatcgtc gagctggatc agctcgagcg gcagccggtg
      361 ccgcagtgta ctggcagcct ggatattatc caacacatca gccgggcgga ccttcaggta
      421 ataatgatac caggagcacc agccgctgat cgcctgatgc tggagcccgg tgcaggcata
      481 ggtttgagcg acagcgtggg caaaggcgcc caggggatct ggatcatcga gctggatagg
      541 gaaaagacag gcccagtcgg tttccatgct ctgcccggga tccagacggg tattgtcccc
      601 gttcgcccac atccgcaaag ccggtgggct gcgatccagg cgggcttcca ggctgccgaa
      661 gtgctgcagc tgagatagaa agcctgccag ccaggcgcgc cgggtggacc ggctgcccag
      721 cacggcaaac atatcactgg cgaaatggcc cggttggccg ggacggggag taccggcatt
      781 cagcaccatc ggctggtgca aaaagcctaa tcgagtatgg tggaaccggt cctggggagc
      841 ataggcgccg gtataagacc aggactgcca gccattgctg aaaaaagcca ggtcgttggg
      901 cggagtctgt ctatgctcgc ccaggtggat cgtggctgcc gggtgatcag cgatcagctc
      961 aattcgctcc atggtgagcg gcacggtgtc ctggttaaca agtttgatct tccacaacaa
     1021 gcaggcggga gattcatgca atgcaaaaac cagctccatc tccagtccgg gagcaggggt
     1081 gaaaaccagg tgcacggtct ggctcattcc taacggcgag gggctgtttc cggggccaga
     1141 gaccgtacaa cccggccagt catccagaag ggtccggcgc tggcctccct ggatatagtg
     1201 caggcgtaag tgcacctggg tgaattcaaa atccgggacc gtctgatagg caagactcca
     1261 ggcagcctga tcgggagcga tggtcagaca cagcgtggag gtctctaagg acagcatggg
     1321 cacgggtgga ttcagcagga gtgatgagca gccagccggt ggtcagagag ccggcggttg
     1381 gtcggtttcg gcagcttcta aggctgccgg gatgtcttca tagattggaa aaacccgatc
     1441 cagcatggtc aggcgcaggg cagtccggat ctgggcgttc gcctggcaga tggtcaacat
     1501 gccgctcgag cggcgcaggg ctttcatgcc ggagaccagg gcgctcaacc cggatgaatc
     1561 catgaactcg acctgtacca ggtcaacgac cagatacctg actccgctgt cgaccaggtc
     1621 caggattgcc tggcgtactg cagctgatga ttcgacatcc atacgtcctg tgggataaag
     1681 aacggcctgg tgaggtgaaa tgagttctga ttttatctgc atgcgtgtac tcccttatgg
     1741 acaaattgaa aggatgtgct cctgaagatt ggtcagtgaa acagacccgt ggcggggtct
     1801 ggccggggaa ggctggtaac ctggctgata aatttttcca ggtgccagtg attacgcccc
     1861 tcggggaggc gggtataagc gcaggtatcc aggaggcggc gggcgattga aagcccatat
     1921 ccaccggtgg gaggatcgct tggcgagaag gccggctcgg cctggagtaa agataagccc
     1981 gtgggcgaat tgaattgaat cccgctatcg atcaggtcta cctggacttt ttcaggaaac
     2041 agcgtcaccc gcccctgaat gcgccccaga tagggcgagt cgtgataggc atggatcact
     2101 atattggcgg taatttcaga tactgccagc tctaaatcgt tgagaaaggc gctgcgctcg
     2161 gcgggaccag agaatgtcag gctggaggca acctggcgga cctgggttgc cagcgtgcgc
     2221 acgctgcgtt tctcggccat gaaaacaaat ggctggacca gggtggcggt ctccgtcccg
     2281 cgggcgcgca ccatgagcaa tacgacatca tcgcgcaggg gcagcttgcc acggtgtaaa
     2341 tccagggcgt tgaaaattaa ttgcagctgc ttatcgattg gagcaggatg acctgcaagg
     2401 agcaaatcga ttaacgcctg ccccccgaac acccggccct gatcattttc cgcctcggtg
     2461 atcccatcgc tgtacaggac caggacatcc ccgggtttta agggaaggtg atgttcagca
     2521 taaacagatt ccgggtaaat tcccaatggc aagccggtac tgggcagctg aagaacatgc
     2581 tgctcagcgg aacgccagag taaagtggtg gtatgcccgg cgctggcata atggagctgg
     2641 aggggccggg tttgaaggac tgccagaaag gaggtgatga aagtttcgga acggtccagc
     2701 tcagcataga tcaggtggtt gattgactgt aacaactcgg ccggctctgt gtgatggtgg
     2761 acttcgcttt tcaaggtggc atggatcatg gccgccagca tggcagcggg gatgcctttc
     2821 ccggctacat ccccgaccat gatcgcccag ccgtcctgca ttccctggac atcataaaaa
     2881 tcaccactga tcagatgagc aggcatcagg ctggcgccaa attcaagctc gggtatatca
     2941 ggcagggttg tgggcaggaa aatggcctgg atctggctgg caaagctaag ctcgagctcc
     3001 aggcgacggt ccgcatcccg gttatggcgg gcaaactggg aagtgatgag catactgatc
     3061 agctcgcctg cgttggtgag cagctgctgg tcagcctggt ttaagctccc ggttgaatgg
     3121 ttgaccagag ccaccaggcc gcgcaattcc ccggtgaggg gcaggggtag gatcagcaag
     3181 ttcttgaggt tcgggatcgc gcgcagcagg gcgcgaggta cgctgtctgc ggagctcaac
     3241 acgctgagcg gcgcggcagt gctggcataa acagccagct tttccagacc ggacggatta
     3301 cgcccgctgg cagtataaat atggggctgg tctgcctccc aaaaggccag gcaaatatcc
     3361 tctgcctcac tcacctggct caatgactgc gccaggcgcg ggtaaagctg ggcaggatcg
     3421 tctgtctgaa aactaaactg cgccaggttg taaaggaagt tgaggtgacc cgaggatgcg
     3481 gatagctgtg tgccaagttg ttgggcggct tgctgggatt ctgccagccc gtccagcaag
     3541 ccggtcatcc gttcgaactg agcttcggca gcagggtctg tcagcaggct agcgatcgag
     3601 atttgggtgg gagagggtcc aagccgacca atcagccagg tctggttttg ccagaggatg
     3661 gtttcaaccg gagctggatg gtgctggagc gtatcgttca gcgcggcaag caaaccggct
     3721 ggcgcggtgt tggacgcgct taacagtgcg ccatcgcccc agaagaacaa ctcgtgccct
     3781 gtcaggtcgc gccacaggcc gccaacctgg gccagcatct cggacattcg ctcaatcatt
     3841 tgtaatactt tactcgaaag tccttcaaat ggcaactaaa cgtgttcagg cacgggtcca
     3901 ctctccccga gcgaccgctg ctccaaatgg gcatggatcc ctcaggcaaa tgcacagatt
     3961 ggaatttacc agggacgcag gctgcggacc gagcgatatc cttccccggt agccaggtag
     4021 cgctgcaggt cgctggcggt ttgcggatcg agatattgct gcaggggctg gggtacctcg
     4081 gcgaactttt gtaaggcaga tttgacctgc gcatcccaat cgggatgcag gcggtcgaaa
     4141 tcttgcgggg taaatttttt gcggcagtcg ggataatggc agaagaggtc catctcctcg
     4201 tcaaagctaa attgagcata gtcattggtg atctcatccc ccggctggag atcgcgcacg
     4261 gcgatatcaa agccgtaacc ggtgctgaca gcactggcat ggcagcagtg attgacgtac
     4321 ttaccgatat cccaacagat gataaaatca ccgttcgggt cagtataggc atattttaag
     4381 atcaaatcgc gatatgcagg atcggcgagc agggggctgg agggtgaaat cctgatatcc
     4441 agactgcagc gagcgtagac gatcgtgcca gccgggataa acgtggtggc aaatacacca
     4501 tagccaacct gagggttgac gaacctgagc tctgtattgg ggtgctgcat gccggagaga
     4561 ctcctcgatg tagggtaggg ttgtttttgc gagctaaccg ggacggtgat tgtcgatcag
     4621 cgggtgtgta tcgccgaaaa gcaggctgcg cacctcactg ccgctcaaga aatcgtgggg
     4681 gaaccccagc tggatcgggc tgagtgaatc cagcttaaag atttgctcgg gggtcagggt
     4741 caaatcaagc gcgccaaggt tatcctggag ctgttccagg tggcgtgcac ccaggat
//
LOCUS       DATMXK010000113         3613 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_20236, whole genome shotgun sequence.
ACCESSION   DATMXK010000113 DATMXK010000000
VERSION     DATMXK010000113.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3613)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3613)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3613
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_20236"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(176..973)
                     /gene="pgeF"
                     /locus_tag="VN363_02060"
     CDS             complement(176..973)
                     /gene="pgeF"
                     /locus_tag="VN363_02060"
                     /inference="COORDINATES: protein motif:HMM:NF014619.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan editing factor PgeF"
                     /protein_id="HWQ83318.1"
                     /translation="MPFHQVDTLRYLTFDLFDQAGIRHAVFTRRGGVSPAPWASLNVG
                     GLVGDLPECVTENRRRSFAALGSSLDSLYDVWQVHSAEVVIAEAPRSGAPHHQADAIL
                     TDHPGVTLYMRFADCTPVLLVDPEHHAVGLVHAGWQGTVKKIAAAAVAAMQARYGSRP
                     GALLAAIGPSIGPHHYPVGPDVVARVEQAFGSQAPALLENPPDRSSGQSGVQFDLWSA
                     NRLVLEAAGVRQIEVSQICTACHLEDWYSHRGEQGRTGRFGALIAVE"
     gene            complement(993..1988)
                     /locus_tag="VN363_02065"
     CDS             complement(993..1988)
                     /locus_tag="VN363_02065"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HWQ83319.1"
                     /translation="MLQVTLYTRQDCHLCEQVKADLESLKDRFPHQLAEIDVDSSPQL
                     RKAFGLEVPVVEIGPYTLKAPVAQSDLALTLAAAQDRLRHIAQIEQEAELAGSVRWTK
                     SDRFTHWISNHYLALLNFLVLLYVGLPVLAPVLMNAGYTAPANVIYRLYSGVCHQLAF
                     RSYFLFGEQAVYPRAEAGLESVMTLQQATGLDESAEGAALLAARRYVGNEQVGYKIAL
                     CERDIFIYGAILLFGLIYAATGRKLPPLPWYLWVGIGMLPIGLDGFSQLLSQPPLNLF
                     PYRESTPFLRSLTGFLFGFTTAWFGYPMVEASMAEARQIMATKLRRIAHQSATPR"
     gene            complement(1988..2605)
                     /locus_tag="VN363_02070"
     CDS             complement(1988..2605)
                     /locus_tag="VN363_02070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWQ83320.1"
                     /translation="MSGSAPSPSPQILRQPAAGRAHPPKTALLGAGVLVGLGLGFLIL
                     AGLGLMGRLPDWFGGGSAAALGPSIDKPALDFTLDNLAGETVSTLDLRGKTVLLNFWA
                     TWCIPCRQEMPMLQAAAEKYAPNLVVLGINIGEEPWRVKKIVYELGLSFEILMDESNQ
                     VETMYRVRAYPTTYLIDDEGIIRAQHVGLLTETQLEKYLERVDLP"
     gene            complement(2602..3531)
                     /locus_tag="VN363_02075"
     CDS             complement(2602..3531)
                     /locus_tag="VN363_02075"
                     /inference="COORDINATES: protein motif:HMM:NF014713.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcdA"
                     /protein_id="HWQ83321.1"
                     /translation="MEIGSLSLSLAFIAGIASFLSPCVFSLVPAYVGYLGGRSAASAT
                     RGVSNRFLTLTHGLMFVLGFSVVFITLGLAASAIGGMLYDLRTWLARIGGVVVIVFGL
                     HMIGILRIPFLEYDLRVQNTPKQGRGYLASFLMGVFFSAGWSPCVGPVLGAILTLSLS
                     GGSIGQGAGLLAAYSAGLAIPFLIASTQIGLVTEAVRKHGKIMRYIEIAMGVLLIVVG
                     VMLLTGRFEQLARYGSFFTSIDEIAVGRLLLIGVVVLAGLGLIPAAIASRKGRSFFDW
                     WVFGAGLFPVALYMVLRIPAHTPTPDSSTLGAG"
BASE COUNT          847 a         1092 c         1061 g          613 t
ORIGIN      
        1 cgacgtaatt ttcacccagc cagcgcgccc cggcctgaac gacctcgcgc agggcgggga
       61 gggagtgtcc cttggtcacc gtaaccagcc ggatcgaagc cggatcacga ccagccaggc
      121 gggcggtgtg ctgaatatcc tcgatcacgc gctgataatt ctcagtaatc atcattcatt
      181 ccacagcgat caaagcgcca aaccgcccgg tgcgaccctg ctcgccgcgg tgcgaatacc
      241 agtcttccag gtggcaggca gtgcagatct ggctgacctc gatctgcctg acaccggccg
      301 cttctaaaac cagccggttg gctgaccaca ggtcaaattg tactccagat tgcccactcg
      361 agcggtcagg cggattttcc agaagcgcgg gcgcctgcga tccgaatgcc tgctccaccc
      421 gggcgaccac gtcgggtcca accgggtaat ggtgcgggcc gatcgatggg ccaatcgcgg
      481 ccaacaaggc cccggggcgg ctgccgtagc gcgcctgcat ggcggcaacc gccgcggcag
      541 caattttttt gaccgttccc tgccaaccgg cgtgaacgag tcccaccgca tgatgctcag
      601 gatcaaccag tagcacgggc gtgcaatcgg caaagcgcat atataaggtc accccggggt
      661 gatcagtgag gatggcatcg gcctggtggt ggggtgcgcc gctgcggggc gcttcggcaa
      721 tcaccacctc ggcgctgtgc acctgccaga catcgtacag gctgtccagg ctgctgccca
      781 gggcagcgaa ggaacggcgg cgattctcgg tgacacactc cggtagatcg ccaaccaggc
      841 cgcccacatt caaggaagcc cagggcgcgg ggctgacgcc tccgcggcgg gtaaataccg
      901 cgtgccggat acctgcttga tcgaatagat cgaaagtcag gtagcgcagg gtatcgacct
      961 ggtggaatgg cataggatcg agggaaagcc gctcagcggg gggtggctga ctggtgagcg
     1021 atccggcgca gcttggtcgc catgatctgg cgagcttcgg ccatcgaggc ttcaaccatc
     1081 ggataaccga accaggcggt ggtgaagcca aacaaaaagc cggtcagact gcgcaagaag
     1141 ggggtgcttt cccggtaggg gaagagattg agcggcggct ggctgagcag ctggctgaaa
     1201 ccatccaggc cgatcggcag catgccgatc cccacccaca ggtaccaggg caggggagga
     1261 agtttccgcc ctgtggcagc atagatcagc ccaaacagca ggattgcgcc gtaaataaag
     1321 atatcccgct cgcacaaagc aattttgtac cccacctgtt cattacccac atagcgccgg
     1381 gctgccagca gcgccgcccc ttcggcgctt tcatccaggc cggtggcttg ctgcagggtc
     1441 atcacgctct ccaatccagc ctctgcccgc gggtagacag cctgctcacc aaataagaaa
     1501 tatgagcgga aggcaagctg gtggcatact cctgaataca ggcgatagat aacattggcc
     1561 ggagcggtat aaccggcatt catcaacacc ggcgccagga ctggcaggcc cacatacagc
     1621 aaaaccagaa aattcaacag cgccagataa tggtttgaaa tccagtgggt gaatcgatct
     1681 gattttgtcc agcgaacgct gccggctaac tcggcttcct gctcgatctg ggcgatgtgg
     1741 cgcagacggt cctgagcagc ggcaagggta agcgccagat cagactgagc gaccggcgct
     1801 ttgagcgtgt atggtccaat ttccaccacc ggtacttcga gcccaaaggc tttccgcagc
     1861 tggggagagc tgtccacatc aatttcggcc agctggtggg ggaagcgatc cttgagagat
     1921 tccaggtcag ctttcacctg ctcgcagaga tggcaatcct ggcgggtgta aagggtcact
     1981 tggagcatca gggtaaatcc acgcgttcca gatatttttc aagctgggtt tcagtgagca
     2041 aaccgacatg ctgggcacga ataatcccct catcgtcgat taaataggtt gtcgggtagg
     2101 cgcgcacgcg gtacattgtt tccacctggt tcgattcatc catcaggatc tcaaagctca
     2161 aacccagctc atagacaatt tttttcaccc gccagggttc ttcaccgatg ttgatcccca
     2221 acaccaccag gttcggggcg tacttttctg cagcagcctg gagcatgggc atctcctggc
     2281 gacacgggat gcaccacgtg gcccaaaaat tgagcagcac ggtcttacca cgcaagtcga
     2341 gcgtgcttac ggtctccccc gccaggttat ccagggtgaa atcgagcgca ggcttatcaa
     2401 tggatggccc aagcgccgca gcactgccac cgccgaacca gtccggcagg cgtcccatca
     2461 agcccagccc ggctaagatc agaaagccca gccccaggcc taccagcacc cctgcgccca
     2521 gcagggcagt tttcggtggg tgggcgcggc cggctgccgg ctgtctgagg atctggggcg
     2581 aaggagaagg agcagatccg ctcacccggc ccccagggtc gatgaatcgg gtgtgggggt
     2641 gtgagcaggt atgcgcagga ccatgtacag ggcaaccggg aacaatccag caccaaaaac
     2701 ccaccaatcg aagaagctgc gccccttacg gctggcaatc gctgccggaa tcaatcccag
     2761 cccggccagg accaccacgc cgatcaacag caagcggcct accgcgatct catcgatgct
     2821 ggtgaagaag ctaccgtagc gcgccagctg ctcgaagcga cctgtcagga gcatgacacc
     2881 gacgacgatc aggagcacgc ccatggcaat ctcaatataa cgcatgattt tgccatgctt
     2941 gcgaacggcc tcggtcacca ggccgatctg ggtcgaagcg atcaggaatg gaattgccag
     3001 accggcggaa taagccgcca gaagtccagc tccctgaccg atcgagccac cgctcaaaga
     3061 gagggtgaga atcgcgccca gcaccgggcc cacgcacggc gaccagccgg cggagaaaaa
     3121 tacacccatc aggaatgaag ccagatagcc gcgcccctgc ttcggggtgt tctgcacgcg
     3181 taaatcgtat tccaggaagg gaatgcgcag tatgccgatc atatgcagac caaatacgat
     3241 caccaccacc ccgccgatgc gggccagcca ggtgcgcaaa tcatagagca ttccgccgat
     3301 tgcagaggct gccaggccga gggtgatgaa caccacgctg aaacccagca caaacatcag
     3361 gccatgcgtc agggtcagaa atcgattgct gacgccgcgg gtggctgagg ccgccgaacg
     3421 gccgcccagg tagccgacat aagcagggac cagcgaaaat acacatggag aaaggaagga
     3481 ggcaatgcca gcaataaaag cgagactgag actgagacta ccgatttcca tgcaatccga
     3541 ctccctaaaa agatctgcgt ttaactttcg acgacctgta taacagttcc caccaaaccg
     3601 gagacagtca tat
//
LOCUS       DATMXK010000114         2654 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_49027, whole genome shotgun sequence.
ACCESSION   DATMXK010000114 DATMXK010000000
VERSION     DATMXK010000114.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2654)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2654)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2654
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49027"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VN363_02080"
     CDS             <1..280
                     /locus_tag="VN363_02080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559420.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF1731 domain-containing protein"
                     /protein_id="HWQ83322.1"
                     /translation="NVTSPNPVQNRDFAKAIGKAVGRPAWIAAPAFAIRLLFGEMATV
                     VLDGQRMIPARLAAEGFKFQYAEPLAALRQVLENNNLRGSTGSTGSIR"
     gene            307..1245
                     /locus_tag="VN363_02085"
     CDS             307..1245
                     /locus_tag="VN363_02085"
                     /EC_number="1.1.1.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR01771.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004459 - L-lactate dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-lactate dehydrogenase"
                     /protein_id="HWQ83323.1"
                     /translation="MKIGIIGSGLVGSTAAYALVMRGIGREVVLVDLNKERASAEADD
                     IFHAVPFAHPVSVRDGDYADLEGCKVVVITAGVGQRPDESRRDLLGRNAAVSNSIVPQ
                     ILQAAPEAVLLVASNPVDVMTHLTARIAAESGLPGSRVMGTGTTLDTARFRVLLGRCL
                     GVDAQHVHAYVIGEHGDSEVLTWSLAAVGGIPLEEYSRMQNQSLCAEDRATIDEQVRR
                     AAFHIIAGKGATYYGVGSAIARIVEVILKDQRSILTVCTPIAEIAGVKNVTLSLPHLV
                     GGEGALSTLYPPVDVQEEQLLHASAEIIAADIALLS"
     gene            1254..2243
                     /locus_tag="VN363_02090"
     CDS             1254..2243
                     /locus_tag="VN363_02090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559950.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWQ83324.1"
                     /translation="MENPAADRSADHVPLGKSGILLSPLGLGAWSWGDRFVWGYGRNY
                     VESDVAQAFQTSREAGINWVDTAEFYGLGQSERLVGRFMGELNADAPRMVVATKFFPL
                     PWKWYAGALVRSLKGSLQRLQFAQVDLYQVHFPRPDGMVERFAQGLVQVLEQGLARAV
                     GVSNYNADQMLRTQIVLNKHGLMLASNQLHYSLLDRKIEKIGLLEVCQKLEISVIAYS
                     PLEMGLLTGKYTPANPPPGSRGQRYSRRLLAEMQPLVQALGDLGEQYGGKTPAQVALN
                     WVMCKGALPIPGAKNARQAAENAGAMGWKLEPNAIKQLDELSDRVLARSEGTA"
     gene            2240..>2654
                     /locus_tag="VN363_02095"
     CDS             2240..>2654
                     /locus_tag="VN363_02095"
                     /inference="COORDINATES: protein motif:HMM:NF013739.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ83325.1"
                     /translation="MIYGSEYYAQYLDLIRNGLPPRSGPRQKVIIVGAGIAGLVAAFE
                     LLHAGHDPLILEGRGRIGGRLYTLREPFSSGLYAEAGGMRIPKSHQLTLAYIEKFDLP
                     TLPFRMGNEQTFSYLHGRRQRTAESLRDPESLGYPI"
BASE COUNT          534 a          762 c          865 g          493 t
ORIGIN      
        1 caatgtcacc agccccaatc cggtccagaa ccgtgacttc gccaaagcga ttggcaaagc
       61 agtcggacgc ccggcgtgga ttgccgcccc ggctttcgcc attcgcctgc tgttcggcga
      121 gatggcaacg gttgtcctgg atgggcagcg gatgatcccg gcgcggcttg cggcagaagg
      181 atttaagttc caatatgccg agccgttggc ggcgctgcgc caggtgctgg aaaacaacaa
      241 tttacgcgga tcaacgggat cgacaggatc gatccggtaa aaacctgtct gaatagggaa
      301 cctgcgatga aaatcgggat tattgggagt gggttggtgg gctcgacggc tgcgtatgcg
      361 ctggtgatgc gcggcatcgg ccgcgaagtg gtactggtgg acctgaacaa agagcgcgcc
      421 agcgctgaag ccgacgatat ttttcacgcg gtgcccttcg cgcacccggt gagcgtgcgg
      481 gatggcgatt acgccgatct ggagggctgc aaggtagtgg tgatcactgc cggggtaggc
      541 cagcgcccgg atgagagccg ccgcgacctg ctggggcgca acgcagccgt atctaacagc
      601 attgtgccgc aaatcctgca ggctgccccg gaggcggtcc tgctggtcgc cagcaatccg
      661 gtggatgtga tgacccatct tacagcgcgc atcgcagctg aatccggctt accgggcagc
      721 cgggtgatgg gcaccggcac caccctggat accgctcgct tccgggttct gctgggccgc
      781 tgcctggggg tggatgcgca gcatgtgcat gcctacgtga tcggcgagca cggcgattcg
      841 gaagtgctca cctggtcgct ggcagcggta gggggtattc cactggagga gtactcccgg
      901 atgcaaaacc agagcctgtg cgccgaagat cgagcgacga ttgacgaaca ggtgcgccgg
      961 gcagccttcc acatcatcgc gggcaaaggc gccacctact acggggtggg cagcgccatc
     1021 gcccggattg ttgaggtcat tctgaaggac cagcgctcga tcctgacggt gtgcacacca
     1081 attgcagaaa ttgctggcgt aaagaatgtc accctttctc tgccgcacct ggtgggtgga
     1141 gaaggcgcat tgagcacgct atacccgccg gtggacgtgc aagaggaaca actcttacat
     1201 gccagcgctg aaattatcgc cgccgatatt gcactgctga gctagaaata tccatggaaa
     1261 accctgccgc agatagatca gccgaccatg ttccacttgg caagagtgga atcctgttat
     1321 ctccgctggg actgggcgcc tggtcctggg gggatcgatt tgtgtgggga tatgggcgga
     1381 attacgtgga aagcgatgta gcccaggctt ttcagaccag ccgtgaagcc ggcataaact
     1441 gggtggacac ggccgaattt tatggtctgg ggcaatcgga gcgcctggtg ggacgtttta
     1501 tgggcgagct gaacgccgat gcacccagaa tggtggttgc gaccaaattt ttccctctcc
     1561 cctggaaatg gtatgcaggt gccctggtgc gttccttaaa gggcagcctg cagcgcctgc
     1621 agttcgcgca ggtggatctg taccaggtgc atttcccccg cccggatggc atggtggaac
     1681 gctttgccca gggtcttgta caggtgctcg aacagggcct ggcgcgggca gtgggcgtct
     1741 ccaattacaa cgccgaccag atgctgcgca cccagatcgt gctcaacaag catggcttga
     1801 tgctggcttc caaccagctg cattacagcc tgctggaccg caagatcgag aaaatcggtc
     1861 tgctggaagt ctgccagaag ctggagatca gcgttatcgc atacagcccg ctggagatgg
     1921 ggctgctgac ggggaaatat actcccgcca accctccccc gggctcgcgc ggccagcgct
     1981 acagccggcg cctgctggca gagatgcagc ccctggtgca ggcgctgggt gatctgggcg
     2041 agcagtacgg cgggaaaact ccggctcagg tagcgctgaa ctgggtgatg tgtaaaggcg
     2101 ccctgccgat cccaggggct aagaacgccc gccaggctgc cgagaatgcc ggcgcgatgg
     2161 ggtggaagtt ggaaccgaat gccattaagc agcttgacga gctcagcgat cgcgtgctcg
     2221 cccgcagtga ggggacagca tgatatacgg cagtgaatac tatgcgcaat acctcgacct
     2281 gatccggaac ggcttaccac cccgctccgg cccacgccag aaggtgatca ttgtaggagc
     2341 cgggatcgcc gggctggtgg cagctttcga gctgctgcat gccgggcacg accccctgat
     2401 cctggaaggg cgcgggcgga tcggtgggag gctgtacacc ctgcgtgagc cgttcagcag
     2461 tgggctgtac gccgaagcgg gcgggatgcg catccccaaa tcacatcagc tcaccctggc
     2521 ctacatcgag aaattcgacc tgcccaccct gcctttccgc atgggcaacg aacagacttt
     2581 ttcctacctg cacggccggc gccagcgcac ggcggaaagc ctgcgcgatc cggaaagcct
     2641 gggctaccca atag
//
LOCUS       DATMXK010000115         2168 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_105633, whole genome shotgun sequence.
ACCESSION   DATMXK010000115 DATMXK010000000
VERSION     DATMXK010000115.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2168)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2168)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2168
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105633"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            292..600
                     /locus_tag="VN363_02100"
     CDS             292..600
                     /locus_tag="VN363_02100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83326.1"
                     /translation="MSKITLGIICGIVFGAVSVATMIPLKLEDKKTALGGAFVNRFAI
                     GFVIGATTLPLPMWGSGLLFGLLLSLPDAIITRSWIPIMALGAIGGVVCGFVIGAFGF
                     "
     gene            complement(711..2111)
                     /locus_tag="VN363_02105"
     CDS             complement(711..2111)
                     /locus_tag="VN363_02105"
                     /inference="COORDINATES: protein motif:HMM:NF012470.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alkaline phosphatase"
                     /protein_id="HWQ83327.1"
                     /translation="MSVQNKNLLNVLASLLTVALLVGFLPAAPTAAGSHQQPKNIIVM
                     ISDGMGYNQSLAASYYHFGKPDRPVYHHFPFQSAMSTYPGAESLGAPDGDPCAGWGYD
                     PALAWSEFDYVRTCPTDSAAAATAMASGVKTYNAAIGVDVNGNRVKNVVEAAEEQGKA
                     TGVVTTVEFSHATPAGFVAHNLSRNDYAGIAQEMIYQSATDVIMGAGHPWYDIDGLPV
                     ITPNTFKYVGGENTWNDLIAGTAGNDADGDGDFDAWTLVQARQDFQNLAFGATPQRVI
                     GVAQVYTTLQQSRSGDGMAVPNQVPLNSSVPSLTEMTLAALNVLDEDPDGLFLMVEGG
                     AIDWASHANQPGRTIEEQADFNLAVEAVVRWVQANSNWGETLLIVTADHETGYLWGPG
                     SNPTWEPLVNNGRGALPGMQFNSSDHTNSLIPFFAKGAAARWFKPYADQEDPVRGAYL
                     DNTAIAQAIFQLLGEQ"
BASE COUNT          405 a          593 c          679 g          491 t
ORIGIN      
        1 ctgccattct gcgcggcaga ggccggctgg gggtcggcgc gtcccgaggc gcaggcggat
       61 aagatccatc ccaggaacac caggagggta aatgatcgga ataaggcttt gaacatactt
      121 cctccgtgaa gggcgtttat atgcgataga atgaaggaga agctgattgc ttacaacatc
      181 atcttagcga attcctgtta atagcttttg atggacaggt aaacgatatg ttatggttgg
      241 tttaaatggc aaaccaccag ttcggactca aagaaaggag tatgaccagc catgtccaaa
      301 ataaccctcg gtatcatttg tggtatcgtc ttcggcgcgg tttctgtagc cacgatgatc
      361 cccctcaaac tcgaagacaa gaaaaccgcg ctgggaggag catttgttaa tcgcttcgct
      421 atcggtttcg tgattggcgc aacaactctg cctttgccaa tgtgggggag tggattactg
      481 tttggattgc tccttagttt gcccgatgca atcattacca ggtcgtggat accgattatg
      541 gcgctgggcg caatcggcgg cgttgtttgc gggttcgtga ttggagcgtt cggcttctga
      601 aggaaatcga gcgtgaccac aaactttaca gtggagccgt attactggct ccactgtttt
      661 gcacgatata gaaattttga tagaggcaga gctggcctag caggctgcgc ttactgctcg
      721 ccgagaagct ggaagatcgc ctgggcaata gcggtgttat cgaggtaggc tccgcgcacc
      781 ggatcctcct ggtcggcata gggcttgaac caacgcgccg ccgcgccttt ggcgaagaag
      841 gggatcaggc tgttggtgtg atcgctcgag ttgaactgca taccgggcag ggcgccgcgc
      901 ccattgttca ccagcggttc ccaggtgggg ttggagcccg gtccccacag gtagccggtc
      961 tcgtgatcgg cggtgacgat gagcagcgtc tcgccccagt tgctgttcgc ctgcacccag
     1021 cgcaccacgg cctccacggc caggttgaag tcggcctgct cttcgatggt cctgccgggc
     1081 tggttggcat gcgaagccca atcgatggcc ccgccttcca ccatcaggaa cagaccgtcg
     1141 gggtcttcat ccagcacatt gagcgctgcc agggtcatct cggtcaggct gggcacagac
     1201 gagttcagcg ggacctggtt tggaaccgcc atgccatcgc cgctgcggga ttgctggagg
     1261 gtggtataga cctgggccac gccgatcaca cgctgggggg tcgctccgaa ggccagattc
     1321 tggaaatcct ggcgcgcctg gaccagggtc caggcgtcga aatcgccatc cccatcggcg
     1381 tcgttcccgg ccgtcccagc gatcaggtcg ttccaggtgt tctcacctcc tacgtatttg
     1441 aaggtgttgg gtgtgatgac gggcaggccg tcgatgtcgt accaggggtg tccggcgccc
     1501 atgatcacgt cggtggcgct ctggtagatc atttcctggg cgatgccggc gtaatcgttg
     1561 cggctcaggt tgtgggcgac gaaaccggcc ggagtggcgt ggctgaattc cacggttgtg
     1621 accacgccgg tggctttccc ctgctcttcg gcggcttcca ccacgttctt cacccggtta
     1681 ccgttcacgt ccacgccgat cgccgcgttg taggtcttca ccccgctcgc catggccgtg
     1741 gcggcggcag ccgaatcggt cgggcaggtg cggacgtaat cgaactcgct ccaggcgagc
     1801 gccgggtcgt acccccagcc tgcacacgga tcgccgtccg gcgcgcccag actctcggcg
     1861 ccgggatagg tgctcatcgc tgattggaag gggaagtggt ggtagaccgg gcggtcgggc
     1921 ttcccgaaat gatagtagct ggcggccagg ctctggttgt agcccatgcc gtcactgatc
     1981 atgacgatga tgtttttcgg ttgctgatgg gatccggctg ccgtgggagc cgctggcagg
     2041 aaccctacca gcagagcgac ggtgagcagg ctggcgagga cgttcagaag gtttttattc
     2101 tggactgaca tttcatattc tccttatcat ttcaggttga gtttaatgaa tttctctcat
     2161 gcaacgtc
//
LOCUS       DATMXK010000116         2731 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_14255, whole genome shotgun sequence.
ACCESSION   DATMXK010000116 DATMXK010000000
VERSION     DATMXK010000116.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2731)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2731)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2731
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14255"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..365
                     /locus_tag="VN363_02110"
     CDS             <1..365
                     /locus_tag="VN363_02110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HWQ83328.1"
                     /translation="SAGRVAFSAVRAARAQGIPVGLLRPITLRPFPYAPLAELAKTVK
                     ALLVVEMNAGQMLDDVLLAAAGHAPVEFYGRMGGMVPYQGEILAEIVRIAGESIPLEG
                     DPRHRWAKRVNYGRNGHN"
     gene            381..1157
                     /locus_tag="VN363_02115"
     CDS             381..1157
                     /locus_tag="VN363_02115"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558881.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent enzyme"
                     /protein_id="HWQ83329.1"
                     /translation="MDITAPTRLVYQRPEALADVPTSYCPGCTHGVAHRLIAEVLDEM
                     GVRENTIGVAPVGCSVFAYNFFNFDFVEAAHGRAPAMATGIKRVLPDRIVFTYQGDGD
                     LASIGMGEIMHAAGRGENITVIFINNAIYGMTGGQMAPTTLPGQKTTSSPLGRDVEQT
                     GFPLRMAELLANMDGAGYVVRRSLHDPKNIRLAKKAIRTAFEAQVRGLGFSLVELLSS
                     CPTNWGMTPMDSLTWIEERMVPVYPLGDYKVAPSLANLRI"
     gene            1173..1733
                     /locus_tag="VN363_02120"
     CDS             1173..1733
                     /locus_tag="VN363_02120"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxoacid:acceptor oxidoreductase family
                     protein"
                     /protein_id="HWQ83330.1"
                     /translation="MQTEIIISGFGGQGALFAGQLLAYAAMDSGYEVTWIPSYGPEMR
                     GGTANCTVIIADEEIGAPTVLNPRAVLALNLPSLDKHEPLVIPGGVLVANASLINRQI
                     TRTDLIAVSIPANEIAEEIGDKRLLNMVMLGALLGKLPVLPVEEIKKAMKAHIPARHQ
                     RLLPANYAALQRGIDFAAQESSPIST"
     gene            1860..2591
                     /locus_tag="VN363_02125"
     CDS             1860..2591
                     /locus_tag="VN363_02125"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83331.1"
                     /translation="MCGNKYLRHRGSPRASSTRASGARLLCGLVLLALIAGCSAPAAL
                     QPPTATPVLPTPTQVPPTATFTPLPTETPVPSATPTETPVPTETQTPTPTETATPTLP
                     PPTPSGEDALYIYYILRESGGPVGCGDTAIKINTGLWRTGDVAQDTAAALKSLFALRY
                     QEYGSLYNGLYASNLKVDSVTFKAFEGIISIRLSGSYGRTEDRCDNSRSRAQIWSTIR
                     QFSDVKTIDILLNGNLLGDILANDH"
BASE COUNT          548 a          836 c          790 g          557 t
ORIGIN      
        1 gctctgccgg gcgggtggcc ttttcggctg tccgggctgc ccgggcgcag ggcatcccgg
       61 tcggtttgct gcgtccgatc acactgcgcc ccttcccata tgcgccgctt gcagagctgg
      121 cgaagaccgt caaagctttg ttggtggtgg agatgaatgc cgggcaaatg ctggacgatg
      181 tcctgctggc tgcagccgga catgctccgg ttgaatttta cgggcgcatg ggcgggatgg
      241 tgccctatca gggtgagatc ctggcggaga ttgtccgcat tgctggcgaa tcgatccccc
      301 tggaaggcga tccacgccat cgctgggcga agcgggtgaa ttatggacgg aacggacaca
      361 attaaggagt gcaaacagac atggatatca ccgctccaac tcgtttagtg taccagcgcc
      421 ctgaggccct ggctgacgtt cccacaagct actgcccggg ctgtacgcat ggggtagctc
      481 accgtctgat agccgaggtg ctggatgaga tgggcgtgcg tgagaacaca atcggcgttg
      541 cgccggtcgg ctgctcggta ttcgcgtaca acttcttcaa ctttgatttc gtcgaggccg
      601 ctcacgggcg cgctccggcg atggcaaccg ggataaaaag agtcctcccc gaccggatcg
      661 tgtttaccta ccagggtgat ggcgacctgg cttcgattgg gatgggcgag atcatgcacg
      721 ctgccgggcg cggcgaaaac attactgtga tctttatcaa taacgccatc tacgggatga
      781 ccggcggtca aatggcgcca accaccctgc ccggccagaa gacgacctcc tcgcccctgg
      841 ggcgggatgt tgaacagacc ggtttccccc tccgcatggc cgagctgctg gcgaatatgg
      901 atggagccgg gtatgtcgtc cgccgcagcc tgcacgatcc caaaaacatc cgcctggcaa
      961 agaaagccat ccgcaccgcc ttcgaggcgc aggtgcgggg tctgggtttc tcgctggtcg
     1021 agctgctttc ctcctgcccc acgaactggg gaatgacccc catggactcc ctgacctgga
     1081 ttgaagaacg catggtcccg gtctatcccc tgggcgatta caaggttgcg ccgagtctgg
     1141 caaacctgcg aatataaagg acggagcccc gcatgcagac tgagatcata atttctgggt
     1201 ttggagggca gggcgccttg tttgccgggc agctcctggc ttatgccgcc atggattctg
     1261 gctacgaagt aacctggatt ccatcttacg ggccggaaat gcgcggtggc acggccaact
     1321 gcacggtgat cattgccgat gaagagattg gcgcgccgac agtgctcaat ccccgcgccg
     1381 tcctggcctt aaatttgcct tctctggata aacacgagcc gctggtgatc ccgggagggg
     1441 tgctggtggc caacgcttcg ctgatcaatc gccagattac ccgcacagac ctgattgcag
     1501 tatcgattcc ggcgaacgag atcgccgaag agatcggcga taagcgcctg ctgaatatgg
     1561 tgatgctggg ggcgctgctg ggcaagctgc cggtgctgcc ggttgaagag atcaaaaaag
     1621 ccatgaaagc tcacattccg gcgcgtcacc agcgtctgct gccggccaac tacgccgcct
     1681 tgcagcgcgg gattgatttc gccgctcaag aatcctcccc gatttccacc tgattaatca
     1741 aaacgacgga cctctggctg ctggagcatt tccagcagcc gggtggtaaa ataccgcttg
     1801 gtgcgcgctg cacaaactgc gcgcatcttt gcgcttgcgc atgattagga gacaaatgca
     1861 tgtgtggaaa taaatatctg cgccaccgag gcagcccacg cgcctccagc acacgcgcct
     1921 ccggcgcacg cctgctctgc ggcctggtcc tgttggcact catagcgggc tgctctgctc
     1981 cggccgcgct acaaccgccg acagctaccc cggttctgcc taccccaacc caggtaccgc
     2041 ccaccgccac atttacgcct ctgcccacgg agacaccggt cccatcggcg acgcctacgg
     2101 agacccctgt ccctaccgag acgcagaccc cgaccccgac agagactgcc acccctacct
     2161 tgccgcctcc cacgccttcc ggtgaggatg cgctgtacat ctactatatc ctgcgcgaat
     2221 ctggcggacc ggtaggctgc ggggatacag ccatcaaaat taataccgga ttgtggcgaa
     2281 cgggagatgt cgcccaggat acggctgcgg cgctcaagag cctgttcgct ttgcgttacc
     2341 aggaatatgg cagcctgtac aatggcctgt atgcctccaa tctcaaagtg gacagtgtca
     2401 cgtttaaagc cttcgaaggg attatttcga tccgtctttc aggcagttat gggcgcacgg
     2461 aagaccgctg cgataactct cgttcgcggg ctcagatctg gtctactatc cgccagttct
     2521 cagacgtgaa gacaatcgac atcctgctca atgggaattt attgggcgat atcctggcca
     2581 atgatcacta aacgccctgc ccggcattca aaattcggag aaaataaatg aaccaccgcc
     2641 tacctatcct gatcctggtt ttcaccctgg tcctgagtgc ctgttcggcg cagaccccgg
     2701 taccgacgct cgcgccaact gccaccagcc t
//
LOCUS       DATMXK010000117         3650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_37803, whole genome shotgun sequence.
ACCESSION   DATMXK010000117 DATMXK010000000
VERSION     DATMXK010000117.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37803"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1221)
                     /locus_tag="VN363_02130"
     CDS             complement(<1..1221)
                     /locus_tag="VN363_02130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83332.1"
                     /translation="MPNLVQSLQGKDLGHLRIISELWGVELNAPDVRQAVQSLLQFML
                     QRKYLSAVLEELPPHALQAWNALLEQDGRLPWAMFARRYGALREMGAARRDREQPYLE
                     IDLASEVLWYRGLIGRAFLDTPSGPEEFAYIPEDLRELLPGAQPRPPEPPGRPAASAE
                     RAVTVLASDWILDHATTLLAALRSGLGLKGAGELDFPAAQPAEIPPLAPELLLVWLGE
                     MGLIDMQQVPVSEPVREFLEAGRGQALASLTRGWMSSLVVNELQLLPDLICEGEWKND
                     PRRARQAIVELVKQLPVGKWWSMAAFVEAVRQQAPDFQRPAGDYDSWFIRRTGSPDYL
                     RGFEHWDEIDGALIRFILAGPLYWLGCVDLALPAENGSKDRRPVSAFRLTGWSEALLG
                     GLAPVLSEETEPSTI"
     gene            complement(1228..1995)
                     /locus_tag="VN363_02135"
     CDS             complement(1228..1995)
                     /locus_tag="VN363_02135"
                     /inference="COORDINATES: protein motif:HMM:NF016163.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF429 domain-containing protein"
                     /protein_id="HWQ83333.1"
                     /translation="MLFSQTTFLGIDPSAGQRPFVYAALDHDLNLLALGQGRLDDMLA
                     FSAGQRTAVVGVCGPRQPNQKVMSRPEIRQALNPPPRPGRWVEFRLAEYELRQRRISV
                     PQIPDRAEKAPNWMQMSFNLFSGLKNLGYQVYPNSEAACQMLEVYPFAAYTALLGVTP
                     FPKYTLEGRLQRQTVLHDLRLKVSDPMRFFEEITRHKLLRGVLPTEQLLSAPELDALV
                     AAYTAWQAALHPADIVLVGDPQEGQIVLPVAELRSQY"
     gene            2212..>3650
                     /locus_tag="VN363_02140"
     CDS             2212..>3650
                     /locus_tag="VN363_02140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWQ83334.1"
                     /translation="MTDSFKLTYATMFNPPEELHTQFDKALAAHKANLGQEYGMIIDG
                     KDVFTAEKFENRSPADTSVVLGVFQKGSEAEAHAALAAARKAFPAWSHMNWQDRVALI
                     RKAAGEIDRRIYEIGAAMAMEVGKNRMEALGDVAEVADLIRYACSQMEKNNGYLVEMG
                     RDPLVGYTSTNYSVLRPYGVWLVISPFNFPGALSGGPTGAALVAGNTVVLKPATDTPW
                     TVRLLAECFRSAGLPEGVLNYVTGPGRTLGQALIDSPEVDGVTFTGSFDVGMGIYRDF
                     ANGKYVRPTILELGGKNPAIVSRHADLDRAATGIIRSAFGLQGQKCSACSRVYIEDAV
                     YDALVPRLVEQTNKLVIGDPTDRKVYLGPVVNKSSYQEFKDFTEELSQAGNILTGGKV
                     LTEGEYANGYFCQPTLVADLPLTHRLWKHEMFLPITTLARVNSLDEAMQLANDVDYGL
                     TAGFYGSKEETSWFFDKIEAGVTYANRPQG"
BASE COUNT          811 a         1097 c         1012 g          730 t
ORIGIN      
        1 gatcgtagaa ggctctgtct cctcggataa gaccggagcc agaccaccca gcaaagcctc
       61 gctccagccc gtcagccgga aagcgctcac cgggcggcga tccttggaac cgttctctgc
      121 cggcagggct aaatccacac agcccagcca gtacagcgga ccagcgagga taaaccggat
      181 caacgcgcca tcgatctcat cccagtgctc aaaaccgcgc aaataatccg ggctgcctgt
      241 gcggcggata aaccacgaat cgtaatcgcc tgccgggcgc tggaaatccg gcgcttgctg
      301 gcgaactgcc tccacaaaag ccgccatgct ccaccattta cctaccggga gctgcttaac
      361 taactccacg atcgcctggc gagcccggcg ggggtcattt ttccattccc cttcgcaaat
      421 taaatccggc aggagctgca gctcattcac caccaggctg ctcatccaac cccgggtgag
      481 actcgccaac gcctggccgc gcccggcttc caggaactcc cggacgggtt ctgagacagg
      541 gacctgctgc atatcgatca gacccatctc gcccagccac accaacaaga gttctggcgc
      601 caatgggggg atctccgccg gctgagccgc gggaaaatcc agctcgcctg cccctttcaa
      661 tcccaacccg ctgcgcaggg ctgccagcag cgtggtggca tggtccagga tccagtcgct
      721 tgcgaggacc gtcacagccc gctcagcaga tgcagccggc cggcctggcg gctctggagg
      781 gcgcggctga gcccctggga gcagctcccg cagatcctcg ggtatatacg caaattcctc
      841 ggggccgctg ggggtatcga gaaaggcgcg cccgatcagc ccgcgatacc acaatacttc
      901 cgaagccaga tcaatctcca gatacggttg ttcccgatcg cgccgcgccg cgcccatctc
      961 gcgcagcgcc ccgtaccggc gggcaaacat cgcccagggt aagcggccat cttgctcaag
     1021 cagagcattc catgcctgca gagcgtgtgg aggcagctcc tcgagaacag ccgataaata
     1081 tttacgttgc agcatgaact ggagcagtga ctgaacggcc tgacgcacgt caggcgcatt
     1141 cagctcgacc ccccagagct cggaaattat gcgtagatgc ccgaggtcct tcccctgcag
     1201 gctttgcaca aggttaggca tggcgactta atattgagac cgcagttcag ccaccggtaa
     1261 gacgatctgc ccctcctgag gatcaccaac cagcacgatg tcggcggggt gtaaggctgc
     1321 ctgccaggct gtataagccg ccaccagcgc atccagctcg ggcgccgata ataactgctc
     1381 ggttggcaaa acgccgcgca gcagcttatg ccgggtgatt tcctcgaaaa agcgcattgg
     1441 atcagatact ttcaagcgta aatcgtggag aaccgtctgg cgctgcaggc ggccttccag
     1501 cgtgtatttt gggaaaggag tcactcctaa taaagcagta taagctgcaa aggggtaaac
     1561 ttcgagcatc tggcaggctg cttcgctgtt cggatatacc tggtagccca gattcttaag
     1621 cccgctgaac aggttgaagc tcatttgcat ccagtttgga gctttttcag cccggtcggg
     1681 gatctggggg acgctgatcc gccgctggcg cagctcgtat tccgccagcc ggaattccac
     1741 ccagcgcccg gggcgaggcg gcgggttcag ggcctggcga atttcaggcc ggctcattac
     1801 tttctgattg ggttggcggg gaccgcatac acccacgaca gctgtgcgct gcccggcgct
     1861 aaatgcaagc atatcgtcca ggcgtccctg tcccaatgcc agcaggttta aatcatgatc
     1921 cagggcagca taaacaaaag ggcgctgccc ggcagaggga tcgatgccca ggaaggttgt
     1981 ctgagaaaag agcatagtgg ccccaatgct gttcaatctg aatgctgaga tgccctgctt
     2041 gcaggcgcac cctcagttta gctcaaaatc agccagcctg caacagtaaa atggtgacct
     2101 gtgtcaccgt cagaagcata cacctgtctt tgactgcaat taaaggctgg actatactat
     2161 acttatcccg ttccgaatct atccccttta ttaaacaagg aggcaatgca aatgaccgat
     2221 tcttttaagc tgacctatgc caccatgttc aatcccccgg aagagcttca tacccagttc
     2281 gataaggccc tggcagccca caaagccaac ctgggccagg aatacggtat gatcattgat
     2341 ggcaaggatg tattcacagc tgaaaaattt gaaaaccgat cacctgccga taccagcgtc
     2401 gtcctgggtg ttttccaaaa agggagcgag gctgaagccc acgctgccct ggctgctgcc
     2461 cgaaaagcct tccccgcctg gagccatatg aattggcagg atcgggttgc cttaatccgt
     2521 aaagctgccg gagagatcga ccggcggatt tatgagatcg gcgcggcgat ggcgatggaa
     2581 gttggcaaga atcgcatgga agccctcgga gatgtagctg aagtggctga tttgatccgc
     2641 tatgcctgct cccagatgga aaagaataat ggttatctgg ttgaaatggg ccgcgatccc
     2701 ctggtcggtt acacctccac caattattca gtcctgcgcc cctatggcgt ctggctggta
     2761 atcagcccgt tcaatttccc gggtgcgctg agcggcgggc caaccggtgc cgcactggtt
     2821 gccggcaaca cggtggtatt gaaaccagct accgatactc cctggactgt gcgtttgctc
     2881 gccgaatgct tccgatcggc tggtttaccc gaaggcgtat tgaactatgt cactggtcct
     2941 ggacgcactc tcggccaggc tttgattgac agccccgaag tggacggcgt taccttcacc
     3001 ggctcctttg acgttgggat gggaatctac cgcgacttcg ccaatgggaa atacgtccgc
     3061 ccgaccatcc tggaattggg cggcaagaat ccggccatcg tttcgcgtca cgccgatctg
     3121 gatcgggctg ccaccgggat catccgctca gcctttggcc tgcaaggtca gaaatgctct
     3181 gcctgttccc gggtgtatat tgaagatgcc gtctacgatg cgctggtgcc caggctggtc
     3241 gagcagacca ataaactggt catcggcgat ccaaccgatc gaaaggtcta tttgggaccg
     3301 gtcgtcaaca aaagctcata ccaggaattc aaagatttca ccgaagagct ctcccaggct
     3361 ggaaatatcc tcaccggcgg caaggtcctg actgaaggcg agtatgccaa tggttatttc
     3421 tgccagccca cgctggttgc cgatctaccc ctgacgcacc gcctttggaa gcacgaaatg
     3481 ttcttgccca tcaccaccct ggcgcgggtg aacagcctgg atgaggccat gcagctggcc
     3541 aacgacgtgg attatggtct cacggctggc ttttatggca gcaaagaaga aaccagctgg
     3601 ttctttgaca agatcgaagc tggcgtaacc tacgccaatc gcccgcaggg
//
LOCUS       DATMXK010000118         3451 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_37797, whole genome shotgun sequence.
ACCESSION   DATMXK010000118 DATMXK010000000
VERSION     DATMXK010000118.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3451)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3451)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3451
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37797"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..611
                     /locus_tag="VN363_02145"
     CDS             <1..611
                     /locus_tag="VN363_02145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558966.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tRNA uridine(34) 5-carboxymethylaminomethyl
                     modification radical SAM/GNAT enzyme Elp3"
                     /protein_id="HWQ83335.1"
                     /translation="TEDLVDLIADIKPTIPRYCRVNRIIRDIPSTNVVAGNKRTSLRQ
                     DVQQKLAERGTACLCIRCREVRSQQVIASTLTREDLVYTTGTSEEHFLAFNTPNDQLA
                     GFLRLSLPNPDAIHTDMGDLDGAAIIREVHVYGQSLPVGSEANGAAQHSGLGTLLLDW
                     SAALASERGYARIAVISAVGTRRYYLDRGFERGELYLVKQLK"
     gene            707..1786
                     /locus_tag="VN363_02150"
     CDS             707..1786
                     /locus_tag="VN363_02150"
                     /inference="COORDINATES: protein motif:HMM:NF012742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000976 - transcription cis-regulatory
                     region binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0045892 - negative regulation of
                     DNA-templated transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWQ83336.1"
                     /translation="MNVRRVTSQDVARAAGVSRTTVSLVLNNVEAQISVDTRQRVLEA
                     AKQLNYHPNASARRLVQGRTRVIGFVEHHSPYQSFADAFMAEVLRGLHAVARQYNYHI
                     IYEPTSDDDRDPDYHVKLVQERHVDGIIYSGPRLNDVALESLAEANIPVVLQGHLPGC
                     PFSSVDIDNISSAKLATDYLIGLGHTQIAMITNGPLIYSAATDRLTGYTLALQAAGIE
                     VNNDWIRIGSFTPDSGFQAMQSLLREKSRPSAVFVASDVVAMGAVEAIHQADLRIPED
                     ISIIGFDNIPWVAYLNPPLTTINVPAHTLGWSAGLLLIQTLENDNFEHDNLLLDTELI
                     VRHSCGNKREAQSPIVTQPPSFSSV"
     gene            1885..>3451
                     /locus_tag="VN363_02155"
     CDS             1885..>3451
                     /locus_tag="VN363_02155"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ83337.1"
                     /translation="MNAKKPIWSLMTMLIVFAMVLSACSSATPTPAPVTEAPAEPVAT
                     EAPVEPVATEAPATGEMPPAGQELADAMDGAYSGTVVSMAGPFTDADQVKFDNSVKAF
                     EDATGIDIQYEGSKEFEAAITIRVDGGDPPDIVDFPQPGLLANMVKKGQVIDLDTIIN
                     PDWLAQNYSQAWLEMGTMEGPDGPITAGVWGRVNGKSSVWYPKDEFDAAGYVVPETWD
                     EMLALTEQIAADGDTPWCIGIESGAATGWTMTDWIEDVMLRTTSLENYDKWVRGELKF
                     DSPEVRRALDYITQIWFNDAYVYGGRAAIATTAFGDAPKPMFEKPPKCWFNRQGNFVS
                     TFFPEEVQAQPGVLFDFFYLPPIDEEYGKPVLGAGDIYAMFNDRPEVRAVIQYFSTGE
                     SVKGWVEAGGAISPHKDSDPSWYADAVTAKVADMLINATAFRFDGSDLMPGAVGAGTF
                     WKYMTDYVSGSIDLDTALKQIDESWPAEATTATGTGGTVSAEPGQELADAMDGAYSGT
                     VVSMAGPFTDADQV"
BASE COUNT          821 a          868 c          991 g          771 t
ORIGIN      
        1 cgaccgaaga tctggtcgat ctgatcgcgg atataaagcc taccattcct cgctactgcc
       61 gcgttaatcg catcatccgc gatattcctt caacaaacgt agtggcagga aataagcgta
      121 ccagtttgag gcaggatgtg cagcagaaac tggctgaacg aggaactgct tgcctgtgca
      181 tccgttgccg ggaagtgcgc agccaacagg ttatcgcttc aacactaacc agagaggacc
      241 tggtctatac caccggcaca tcagaagaac acttcctggc atttaatacc ccgaatgacc
      301 agcttgcagg atttttaagg ctgtctttac ccaatcctga tgctatccat acggacatgg
      361 gagacctgga tggagcagct atcattcgtg aagtgcatgt ctacggtcag tcactgccag
      421 ttggaagtga agcgaacggt gcagctcagc actccggtct ggggactctt ttgcttgatt
      481 ggtcggctgc actggccagc gagcggggct acgcacgcat cgcggtgata tccgcggtgg
      541 gtaccagacg ttattatctc gaccgtggtt ttgaacgtgg agaattatat ttagtcaaac
      601 aactcaaata aacctgttga ctttacctcg aaattctgct atattactaa cacgagttag
      661 taacgcgtgt tagtgagagg ggcgaatttt tgaggacaca aaccgaatga atgtcaggcg
      721 ggttaccagc caggatgtcg ccagagcagc cggtgtgtcc aggaccacgg tgtcattggt
      781 tctcaataat gttgaagccc agatcagtgt agatacccgc caaagggtcc tggaagcggc
      841 caaacaactg aactatcatc ctaatgcctc tgctcggcgg ctcgttcagg gccggacgcg
      901 cgtaattggc ttcgttgaac atcattcccc ttatcaaagc tttgcagatg cttttatggc
      961 tgaggtgttg cgtggtttgc acgctgttgc ccgccagtac aactatcata ttatttatga
     1021 gccgacctca gacgatgacc gtgatccgga ttaccatgta aaattggttc aggaacggca
     1081 cgtggatggg attatttact caggaccacg cttaaacgat gtcgccctgg aaagcctggc
     1141 tgaggcgaac atacctgttg tattacaagg acatcttcca ggatgccctt tttcgtcagt
     1201 cgacatcgat aacatctcca gcgcaaaatt agcaactgat tatttgatcg gattagggca
     1261 cactcagatt gcgatgatca ccaacggccc tctcatctat tccgctgcaa cagatcgcct
     1321 gacagggtat accctggcgc tgcaagctgc ggggattgag gtaaataatg actggatccg
     1381 gattggctct ttcacaccgg acagcggttt tcaagcgatg cagagcctgc tccgggaaaa
     1441 aagtaggccc agtgctgtat tcgtcgccag cgatgttgtg gcaatgggcg cggtcgaagc
     1501 aattcaccag gcagacttga ggattcccga agatatttcc ataattggtt ttgacaacat
     1561 accctgggta gcttatttga atcctcctct gacaacgatc aatgtacctg cgcataccct
     1621 cgggtggagc gcaggtcttc tgttaattca gaccctggag aatgataact tcgagcacga
     1681 taatctctta ctggataccg agctaattgt caggcattca tgcgggaata agcgagaagc
     1741 tcaaagcccg attgtcactc aacccccatc attcagctcg gtgtaaaatg tatcaaggag
     1801 gtgaaataac ctgacagatc cagccttgtg tcttgttcta ctgtatccaa cttaacttgt
     1861 gtaacaagga gtagaaagaa gaacatgaac gcgaaaaaac ctatatggtc gctgatgact
     1921 atgctgatag tctttgcaat ggtcctgtcg gcatgctcga gtgcgacacc aactccagct
     1981 ccagtaactg aggctcccgc tgaaccagtc gctacagaag cgcctgtcga gccagtcgcc
     2041 actgaagcgc ctgccaccgg agaaatgccg cccgctgggc aagagctggc agacgccatg
     2101 gatggtgcct actctggcac agtggtgagc atggcgggtc cgttcacgga tgcggaccag
     2161 gtgaaattcg acaactcggt gaaggcattt gaagatgcga ccgggatcga cattcagtac
     2221 gaaggctcga aggaatttga agctgcgatc acgatccggg tggatggcgg ggatccgcca
     2281 gacattgtgg actttccgca gccgggcttg cttgcgaaca tggtgaagaa gggtcaggta
     2341 atcgacctgg acacgattat caacccggac tggctggcgc agaactacag ccaggcgtgg
     2401 ttggaaatgg ggaccatgga agggccagac gggccgatca cggctggggt atggggtcgt
     2461 gtgaacggca agagctcggt ctggtatccg aaggacgagt ttgacgccgc cggatacgta
     2521 gttcccgaga cgtgggacga gatgctggcg ctgaccgagc agatcgcggc tgatggcgat
     2581 acaccctggt gcatcggcat cgagtcagga gctgcgacgg gctggacgat gacagactgg
     2641 atcgaagatg tgatgctgcg gaccacttcg ttggagaact acgacaagtg ggtgcggggc
     2701 gagttgaagt ttgactcacc ggaagtacgc cgggcgctgg attacatcac gcagatctgg
     2761 ttcaacgatg cgtatgtgta tggcgggcgg gctgcgatcg cgacgacggc ttttggcgat
     2821 gcgccgaagc cgatgttcga gaaaccgccc aagtgctggt tcaaccgcca gggcaacttt
     2881 gtgtcgacct tcttccccga agaagtccag gcacagccgg gtgtgttgtt tgacttcttc
     2941 tatctgcctc cgatcgacga agaatatggc aagccggtgc tgggtgctgg cgatatctac
     3001 gccatgttca acgaccggcc agaagtacgg gcagtaatcc aatacttctc gaccggcgag
     3061 tcggtgaagg gttgggtgga agctggtgga gcgatctcgc ctcacaagga ctctgaccca
     3121 tcctggtacg ctgatgcggt gacggccaaa gttgcggaca tgctgatcaa tgcgacagct
     3181 ttccgcttcg acggctcaga cctgatgcct ggtgcagttg gcgcaggcac attctggaag
     3241 tacatgaccg actacgtcag cggctccatt gacctggata ccgctctcaa gcagattgac
     3301 gaatcctggc cggctgaggc tacgaccgcg accggcacag gcggcactgt ttcggcagag
     3361 cctgggcaag agctggcaga cgccatggat ggtgcctact ctggcacagt ggtgagcatg
     3421 gcgggtccgt tcacggatgc ggaccaggtg a
//
LOCUS       DATMXK010000119         3839 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_160881, whole genome shotgun sequence.
ACCESSION   DATMXK010000119 DATMXK010000000
VERSION     DATMXK010000119.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3839)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3839)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3839
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160881"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(15..494)
                     /locus_tag="VN363_02160"
     CDS             complement(15..494)
                     /locus_tag="VN363_02160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559628.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWQ83338.1"
                     /translation="MDAKVTWKGKMTFSGTSDSGFELPLGTYPELGGDNDGFRPMELL
                     AIGLGGCTAMDVISILQKKRQQITDFEVDIKAKRASEHPKVFTQARVTYVITGQAVDE
                     SAVVRAIELSVTRYCPATAMFSQVMPIETAYTIYEAGENGERQLVKSGTYNRNPDQD"
     gene            complement(496..1086)
                     /locus_tag="VN363_02165"
     CDS             complement(496..1086)
                     /locus_tag="VN363_02165"
                     /inference="COORDINATES: protein motif:HMM:NF012787.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWQ83339.1"
                     /translation="MHKKPLKKTGRKRTQNSLPLVLAGMGLIIFGVAAAIIVFEETNR
                     NQAQAQAGEVLPQIVNYPAPELQLVDVNGQTVDLKAYTGQVVLLNNWATWCPPCRAEM
                     PALQVYHTAHQEQGFTVVAVEAGDPIEQVRQFVDEYGLTFPVWVDTRQQALAAFRNMA
                     LPNSYVMDRAGQVRLAWNGAISLETLDKYVTPLLEE"
     gene            complement(1178..1408)
                     /locus_tag="VN363_02170"
     CDS             complement(1178..1408)
                     /locus_tag="VN363_02170"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWQ83340.1"
                     /translation="MLLAHQMAMESPLIEAEMIEATEFPVLSNQHHVSSVPQTTINNG
                     AGTAVGAVPEGQLLAEIIRVVGAPQVKVATQV"
     gene            complement(1427..1873)
                     /locus_tag="VN363_02175"
     CDS             complement(1427..1873)
                     /locus_tag="VN363_02175"
                     /inference="COORDINATES: protein motif:HMM:NF024590.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin family protein"
                     /protein_id="HWQ83341.1"
                     /translation="MAEKLLNEQIVRQIQEIFTNLKEPVQVLYFGQEKNCDYCADTHQ
                     LVEEVTAISDQLYLDVYDIERDAEIAQRYHVDKAPGLVIAGRDGDQIVDYGVRYAGIP
                     SGHEFGSLIHDLVNVSRRDSGLSQETREFLKTLETPVLLQVFVTPT"
     gene            complement(2024..2272)
                     /locus_tag="VN363_02180"
     CDS             complement(2024..2272)
                     /locus_tag="VN363_02180"
                     /inference="COORDINATES: protein motif:HMM:NF024881.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWQ83342.1"
                     /translation="MAHENCRHLLGSLSEYIDGALGDEICTEIEKHLAGCENCRVVVD
                     TLKKTVYLYHVTNENPAVPDGVRNRLLQRLELKDLVEK"
     gene            complement(2272..2901)
                     /locus_tag="VN363_02185"
     CDS             complement(2272..2901)
                     /locus_tag="VN363_02185"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ83343.1"
                     /translation="MSDEKIITDDGFSLTALQTGDKAEFARLVDAYYPVIYRLALKIL
                     GNTQDAEDILQETFIKAFRNIADFNGRSRLSTWLYRIATNEALMLIRRRKPDTISIEV
                     PREGDEEDKEPLEIVDWCCMPERELLSSESRQRLDQAIESLPPSLRVVFVLRDIEGLS
                     TLETGEVLHLSETAVKTRLSRARLRLRKELTQYYGDRMRRKPVAEEGGR"
     gene            3104..>3839
                     /locus_tag="VN363_02190"
     CDS             3104..>3839
                     /locus_tag="VN363_02190"
                     /inference="COORDINATES: protein motif:HMM:NF014003.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAM-dependent chlorinase/fluorinase"
                     /protein_id="HWQ83344.1"
                     /translation="MSIVTLTTDFGLKDGNVGVMKGVIWKLAPRAHIADLSHQINPQN
                     IAEAALILSRSAPYFPSDTVHIVVVDPGVGTARRPIAARIDEQLFVGPDNGFLTPLLE
                     RAEQENLFAEIIHINRPQFWLPAVSHVFHGRDIFAPVGGHLASGTPLLALGNKITDLV
                     RLPLSHPQQFSGGYQGEIIHIDHFGNLSTNLRVADLAGHTSLSIQLRGAEIPGLYHTF
                     GELPPGSLMALIGSTGSLIISVVNGSA"
BASE COUNT          845 a         1144 c          897 g          953 t
ORIGIN      
        1 cctggctgag gtggctaatc ctggtcaggg ttgcggttgt aagtcccgct tttgaccagc
       61 tgccgctccc cgttttctcc agcctcataa atcgtatagg ccgtttcgat cggcatcacc
      121 tgggagaaca tggcagtcgc agggcaataa cgggtcacgg agagctcgat cgcccgcacc
      181 actgcgcttt catccaccgc ttgcccggtg ataacatatg tgacccgggc ctgagtaaag
      241 actttggggt gttcgctggc ccgtttcgcc ttaatatcca cctcaaaatc ggtgatttgt
      301 tggcgttttt tctgcaggat tgagatgaca tccatggctg tacagccccc gaggccaatc
      361 gccagcagct ccatggggcg gaagccatcg ttatccccgc ccagctccgg ataggttccc
      421 aggggcagct caaaccccga atcagacgtt ccactgaacg tcatttttcc tttccaggta
      481 acttttgcat ccatgttatt cctccaacag gggtgtgacg tacttatcca gggtctccag
      541 gcttattgcc ccattccacg ccaggcgcac ctgcccggcc cggtccatga catacgaatt
      601 tggtaaggcc atgttgcgga aagctgccag tgcctgctga cgggtatcaa cccacaccgg
      661 aaaagtcaga ccgtactcat cgacaaattg gcgcacctgt tcaatcggat cgccggcttc
      721 cacagctacc accgtaaaac cctgttcctg atgagccgta tggtacacct gtaaagctgg
      781 catttcggcc cggcagggtg ggcaccatgt cgcccaattg tttaacagca ccacctgacc
      841 ggtataagct ttcaaatcca cagtctgccc attcacatca acgagttgca gctcgggcgc
      901 gggataattg acaatctgag gtagaacctc tcccgcttga gcctgggcct ggttccgatt
      961 ggtctcttca aacacaataa tagccgctgc caccccgaaa attatcaacc ccatgcctgc
     1021 cagtaccagc ggcaagctgt tttgcgtgcg ttttcgccca gtctttttga gaggcttttt
     1081 atgcatcctt gctcccgttc aaaatccgta gagcccaggg gataatcatc ctgggctcta
     1141 caattttacc ggtaaaatat tacctgccag tacaccgcta aacctgtgtg gctactttta
     1201 cttgtggtgc ccccaccacc cggataatct ccgctaacaa ctgcccttcg ggcacggcac
     1261 cgaccgctgt cccggcgcca ttattgatcg tggtctgcgg cacgctgctg acatggtgct
     1321 ggtttgagag caccggaaac tccgtggctt ctatcatttc cgcttcgatg agcgggcttt
     1381 ccatcgccat ctggtgagca agcagcacgg cttgcgggca ccagggtcag gtaggggtga
     1441 caaatacctg cagtaagacg ggcgtttcca gtgtcttcag gaactcacgg gtctcctggc
     1501 ttaaccccga atcgcggcgt gagacattca ccagatcgtg gatcagcgag ccgaattcgt
     1561 gtcccgaggg gatgcccgca taacggaccc cataatccac tatttgatcg ccatctcgcc
     1621 cggcgatcac cagcccgggg gctttatcca cgtgataacg ctgggcaatc tctgcatcgc
     1681 gctcgatatc gtacacgtcc aggtacagct gatccgagat tgccgtcact tcttccacca
     1741 gctggtgagt atctgcacag taatcgcagt tcttttcctg tccaaaatac aatacctgaa
     1801 ccggctcttt caagttcgta aatatttcct gaatttgcct tacgatctgc tcatttaata
     1861 atttttccgc catccatctc tcctaactgc accccaccga ggcgcttgat taaatatcgt
     1921 tcatccctta tttcagatgc aaaaacactt gaaaggttgc agtggacaaa aaaggagttg
     1981 caggtgaaga cctgcagctc cttaattcag tcagcatgac tttctatttt tcgacgagat
     2041 cctttaattc cagacgctgc aacaggcggt tgcgtacgcc atccggtacc gcaggattct
     2101 cattggtgac atggtacagg taaaccgtct tcttcaaggt atcgacgacc acccgacagt
     2161 tttcacaacc cgccaggtgc ttctcgatct ctgtacaaat ctcatctcct aaagccccat
     2221 cgatgtattc cgaaagtgag cctaataaat gcctgcaatt ttcgtgagcc atcagcggcc
     2281 tccttcctcg gcaacgggct tacgcctcat tcgatcacca taatactgcg tcaactcttt
     2341 tctcagtcgc agccgcgctc gagataagcg ggtctttacg gctgtttcac tgagatggag
     2401 tacctcaccc gtctcaagtg tagataatcc ctcaatatct cggagcacaa acacaacccg
     2461 taaactgggc ggcaggctct cgattgcctg gtccagacgc tgtcgagatt cactcgagag
     2521 cagctcccgc tctggcatac agcaccaatc tacaatctcc aagggttctt tgtcttcctc
     2581 atcgccttcc cgagggactt cgatcgagat cgtatctggt ttccgccgcc ggatgagcat
     2641 cagagcctca ttggttgcga tccggtacaa ccatgtggag agacgggatc gcccattaaa
     2701 atcagcaata ttgcgaaacg ctttgatgaa ggtctcttgc aggatatctt cagcatcttg
     2761 cgtattaccc aaaatcttca aagccaggcg ataaataacc ggataataag catccaccaa
     2821 ccgggcaaat tcagccttat cgccggtctg aagagctgtt aatgaaaaac catcatccgt
     2881 aataattttc tcgtcactca ttggatgcat cctgacccaa aaggttacgc aaggcttggg
     2941 tccagaaatt gtaacacacg ccaaacatcc tgcctgtacc cttaccaaac ctccaccaca
     3001 cccacttagc ttactgcttg acgcacagac atcgccgtgc tatagtccaa agcacgtacg
     3061 caccccgcaa gttcttttcc agtcccatcc tgaggaggcc aagttgagta tcgtcaccct
     3121 gacgacagat tttggcttaa aagacggcaa tgtaggtgtg atgaaagggg tgatttggaa
     3181 gctggctccc cgcgctcaca ttgctgatct cagtcaccag atcaatcctc agaatattgc
     3241 tgaggcagcc ctgatccttt cccgctctgc gccttatttt ccatccgata ccgtccatat
     3301 tgtggtggtc gatcctgggg ttggcactgc gcggcgtcct atcgctgcgc gcatcgatga
     3361 gcagctcttc gtcggcccag ataatggttt tctcacccca ttgttggagc gggctgagca
     3421 agagaattta ttcgccgaga tcatccacat aaaccggcct cagttttggc tgccggctgt
     3481 cagtcacgtc ttccacggca gggatatctt cgcccctgtt ggcggtcacc tggcctccgg
     3541 tacgcctctg ttggctctgg gcaacaaaat cacagatctg gtccgccttc cgctgtcgca
     3601 tccccaacag ttttccggag gttatcaggg ggagatcatc catattgatc atttcggtaa
     3661 tctctccacc aacctgcgcg tggctgacct tgccgggcac acttcccttt ccattcagct
     3721 gcggggagcc gagattcctg gcctgtacca taccttcggc gagctgccac caggctcact
     3781 gatggctctg atcggctcca ccggcagcct gatcatctct gttgtcaatg gcagcgccg
//
LOCUS       DATMXK010000120         9715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_2897, whole genome shotgun sequence.
ACCESSION   DATMXK010000120 DATMXK010000000
VERSION     DATMXK010000120.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2897"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..923
                     /locus_tag="VN363_02195"
     CDS             <1..923
                     /locus_tag="VN363_02195"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ83345.1"
                     /translation="YALCAVANEVSRRHFNNLGEIYAQIGLDFAPCPGNCEFCSFGSD
                     HGNVKSVIEYPAENIARAAKICEDQGANGIYLMTTARYRFEKFLDVAREVRQAVSPEF
                     PMVANIGDFNEDHARELADLGFKAVYHAVRLNEGKGTGFSIKRRVKSIAAARKAGLAL
                     HFCVEPVGPEHTPEEQVDLMFLGRELGVTFSGAMRRVCVPGTKVADRGNITWWSLART
                     VAVCRLVMMDTVLGHCTHEPNLPSLLAGANLLWAEMGPNPRDDRPDTENSRGMGIKQC
                     QELLTEAGYDIRRGPAITAIATPERNSVRT"
     gene            1133..2083
                     /locus_tag="VN363_02200"
     CDS             1133..2083
                     /locus_tag="VN363_02200"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWQ83346.1"
                     /translation="MNLNPAQMFKAAREILVQKGRIDPEEYDVMRVDREWNFFSIYRA
                     TYDIHFDDLKRTDYDTLFFPGCTLASYSPELTRAAFHWLEKRGLQVGFTELCCGKPLD
                     SIGLSGTANHYLDELRNELETSGARQIITACPNCENSLRSKLPGLDIHSIYGLMLDAG
                     VHVTGEGTLTFHDSCPDRYGSSNPQDVRQLLSGFQQVEMKSRGRDTICCGSGGIVSMV
                     DPELCASRAQKRMAEFFSTRADTCVTACMACSHRLAQNIRAGQVRHCLEYVLDIQVDY
                     EQVEKNTIAMWEGRTGEINLARLAQAHELSAEEEKSGENA"
     gene            2076..3398
                     /locus_tag="VN363_02205"
     CDS             2076..3398
                     /locus_tag="VN363_02205"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ83347.1"
                     /translation="MPETINVLDHTESLCPICLKIIEARIVVQNGAVHMEKVCPDHGA
                     ANAYLWPDPDHYLWMNSFRLPFTQPQAEVPLQDGCPLDCGLCPAHLRHATLVEIEVTQ
                     RCNLRCPVCFMSAGAELPDPDMYTLRAMLEATYRQSGGQTSVQITGGEPTVRKDLPEI
                     IRLVKETGFSAVEVNTNGVAIGRDPAYIQTLVDAGISGIYLQFDGLTQAVYTQIRGAD
                     LLNTKLQAIEYCRAAGVQVVLAMTIIWGVNHNQIGDVLDFALRNQDVIVGVAFQPAFT
                     SGRFSVQPERRLTMGDVIFMLSEQSQGLIEPYDLWPLSCSHPLCSCATYIIEDQGTRK
                     PFTRLITSQEYIQGFDSHSPQGSVFADIAARQFPAISHGLSVVIMNYMDAMNIDLKRL
                     KECSMTVTMENGRHIPFCAYQLTDLNGKRLYPMWGRVEGQSVREAGNA"
     gene            3391..5616
                     /locus_tag="VN363_02210"
     CDS             3391..5616
                     /locus_tag="VN363_02210"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ83348.1"
                     /translation="MPEVKRVRANCRFCGYLCGLIATVEDGRVIAVEPDPSRYPYDAS
                     VQRGCRRWRTNLEFLDHPDRINFPLKRLGERGSGQWEQVSWEEAVDDIGARLQLLKDR
                     HGPETLATSIGGPHATYWPLHRFMNLFGSPNNIGIGQICWNPGVWINTLTYGWPLENE
                     LDPQTTDCAFLWGTNPAESDNSLFWRAILEYRHTGKPLIVVDPRKTKTAAQATLWLPI
                     NPGTDAFLALGLLHQILTEKLYDHRFVESWCSGFEHLVEHVAQYSPEFVAKITGVNAE
                     QIVEAARLYAMNAPASLISGRGIDQLGANSIPTHQALVLLRAVTGNVDVPGASHLGEM
                     PAFTPEVDLELSDELPEIQRQKGLNRQLLQSYSGYARVRQVTGRAGRRLPMRYLTSAH
                     PNLVWQAMLTGEPYPIRALVVMGSNPLLTQADSRLIYRVLKSLDLLVTLEYFKTPTAM
                     LADYILPAAGGLERPLFQTHAGIANIAYGGDQAVEPYYERRTDFYFWRELGLRLGQAE
                     RWPWETFQAALEATLTPAGLSWEQFCESGLYYQPGPYQKHERLDPLSGEKEGFGTPSG
                     KVELYSELLAELGYAPLPSPPPPRPPEGSFPLLLTTGARKQPFYASSYRQVVSLRQMH
                     PEPWAEMSETTAARLGLEDGCPIIVETERGQARFIVKIAEMRPDVVSVEYGWWFPDLP
                     ACEPDLGGVWISNANMLTSADIRTSDPVIGTWTYNGLPCRVLPGADEPVKQEMEIVNE
                     R"
     gene            5606..6463
                     /locus_tag="VN363_02215"
     CDS             5606..6463
                     /locus_tag="VN363_02215"
                     /inference="COORDINATES: protein motif:HMM:NF021365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2064 domain-containing protein"
                     /protein_id="HWQ83349.1"
                     /translation="MSGKKHAFLLFTKAPAPGVTKTRLTEARGGILTPEEAADFYRAA
                     LLDVAELAFQALAELNQAAQTGANGSDPDRYDFIVSSPPKTDQAILEALFSENEPWPA
                     PVQFINDHGKTFDEHFDDAFQQLFDQGYHSVVSIGGDLPTMPVSHIVQAFQWLEYYDT
                     YSEKSGFVQAPCQECGVSLVGYTAKTPMDSTGVFYNLDGVPALDAYIAKAAQRGVPVA
                     SLTPVADVDDMRDMAHTISLLHGIAYSSQFQPGLFVARRTLDWIQRTRIVINTPPNAE
                     HDPRERIDA"
     gene            6456..7454
                     /locus_tag="VN363_02220"
     CDS             6456..7454
                     /locus_tag="VN363_02220"
                     /inference="COORDINATES: protein motif:HMM:NF014635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF169 domain-containing protein"
                     /protein_id="HWQ83350.1"
                     /translation="MPEIELAEERCISCGLCVDFCPVNVLEMIEIEGRLLPAAVRVED
                     CWACDTCVGQCPTGALRVVEAYEPTLVPAAKHPGCNGHEPVAAGLTTEQQALYQSWSE
                     TLMRVLRLRWSPVAINLIPAGAPLPDAPQPREKLRYCQSLMAARRGKTLLMPAHCHAC
                     PDGTSILGLTEIPPKLASGELYIRFGKLATIEAARQMVAERPRLEPRSVQATMVAPLP
                     QAILPPDVVAIIAQPEQMMWLCMAASFYTGRRFDFKVSGYNAQCVETTLFPYTSGKIN
                     ISLGCYGCRASSDIGDDLMFMGVPVDQMPTLIQGLKELGKKAIPDSRNKIYLEPLT"
     gene            7451..8095
                     /locus_tag="VN363_02225"
     CDS             7451..8095
                     /locus_tag="VN363_02225"
                     /EC_number="1.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016646 - oxidoreductase activity, acting
                     on the CH-NH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWQ83351.1"
                     /translation="MSRLVLQPDRDLRRKEICGSEIAALLNPRLAVLVSCCDLTGKPN
                     ILSVAWHSPLSHKPPLVGIAIHPRRYSHDLIRAGQEFIVNVVGMAFEDAVQICGSYSG
                     ASTDKAKLAGLSFSPARTVRPPYLTNALGHLECRVWGEVPVGDHTLFIGQVQFAEALE
                     ACFDACWDPSKADVLLCLQRDRLVTLGGGKSLPQGSGSAAEDATWDLEELICQP"
     gene            8083..9099
                     /gene="arsS"
                     /locus_tag="VN363_02230"
     CDS             8083..9099
                     /gene="arsS"
                     /locus_tag="VN363_02230"
                     /inference="COORDINATES: protein motif:HMM:TIGR04167.1"
                     /note="Some members of this family are selenoproteins;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenosugar biosynthesis radical SAM
                     (seleno)protein ArsS"
                     /protein_id="HWQ83352.1"
                     /translation="MPTVTSKYGDEEALEALVPSFEDRLGQETASLVRHSLDSLQINV
                     GKQCNQACLHCHVEAGPQRVEIMDQRTAELAIEFACLSGAQTVEITGGAPELNPSFRY
                     MVGQLRQAGLRVIDRSNLTVFFEPGQEELPEFLAGMKVELVASLPCFTEKNVDQQRGR
                     GVYRKSAQALLKLNELGYGKEDSGLDLNLVYNPLGAYLPAPQAELEADYKRELATRFG
                     IRFNHLYTITNIPIGRFAHILARQGEMQAYARLLVESFNPATLDNLMCLRQVSLSWDG
                     LIYDCDFNQMLDMRLSNGRVFRLGKAPAAQIAASLIGKPIRTGAHCYGCTAGFGSSCA
                     GALA"
     gene            9116..9649
                     /locus_tag="VN363_02235"
     CDS             9116..9649
                     /locus_tag="VN363_02235"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ83353.1"
                     /translation="MKDPWVDVKEAMTIMSWEESADSGMEGAVAGSGNLSRPWLRLGR
                     PEDAPALQQLIAEAGMYAEVDPLECLVADTPHGVIGFARVEFFEDVPYLRPVVVAPPY
                     QGHGVGRRLVEGLLADYPGLYVVSRGKAAGFYQRLGFQALPWSAVAADHQEECDLCPE
                     RSQCCPVGLHAGRNEEC"
BASE COUNT         2094 a         2592 c         2843 g         2186 t
ORIGIN      
        1 tgtatgcgct gtgtgcagtc gccaatgagg tttccaggcg gcattttaat aatctaggtg
       61 aaatctatgc tcaaatcggg ctcgattttg caccctgccc cggaaactgc gaattttgct
      121 ctttcggctc cgatcacggg aatgtaaagt cagttattga atatcctgcg gagaacattg
      181 ctcgggctgc caaaatttgc gaggatcaag gcgccaatgg aatttatttg atgaccaccg
      241 cccgataccg ttttgaaaag tttttagatg tggcccgaga agtgcgacag gcggtttcgc
      301 ccgagtttcc gatggtggcc aatatcgggg atttcaacga agatcatgcc cgcgagctgg
      361 ctgatctcgg tttcaaagcg gtttatcacg cagttcgtct caatgaaggc aaaggaacag
      421 gtttttcaat caaacgtcgc gtaaagagta tcgcggcggc acgcaaagcc ggccttgcgt
      481 tgcacttctg tgttgaacca gtggggccgg aacatacacc cgaggagcag gtcgatttga
      541 tgttcttagg acgtgaattg ggcgttacat ttagtggagc catgcgcaga gtgtgcgttc
      601 ctggcacaaa ggttgccgat cgcggcaata tcacctggtg gtcgcttgcc aggacggtgg
      661 cagtctgccg tttggtgatg atggataccg ttcttggcca ctgcacccat gagcctaacc
      721 tcccgtctct tttggcggga gccaacctgt tgtgggctga gatgggaccc aacccgcgcg
      781 atgaccgacc ggatacggaa aactcacgcg gaatgggaat taaacagtgc caggaactcc
      841 tcactgaagc tggttacgat atacgccgcg gtccggccat cacggctatc gctacgcctg
      901 agcgaaattc agtcaggaca tgagcactaa gcacgctaat caggttattg agaaattttc
      961 cgacgaatgc atcctgtgcg gtgcatgtct gtcagcctgt accctgctct ctgatttggg
     1021 cgagaaccca ggtgaaatcg cccaggcgat ttcgcgtgaa gaggtctctg atgaattagt
     1081 ggctgcaatt caacgctgtg ctctgtgcgg gtattgcagt caggaatgtc tggtgaactt
     1141 aaatcctgca cagatgttca aggcagccag agaaatactt gtccaaaagg gacggatcga
     1201 ccctgaagag tatgatgtca tgcgggttga ccgtgaatgg aatttctttt ccatctaccg
     1261 cgcaacttac gatatccatt tcgatgatct aaagcgaaca gactacgaca ccctgttttt
     1321 ccccggttgt actctcgcaa gttactcccc cgaattaact cgcgccgctt tccattggct
     1381 cgagaagcgc ggtttgcagg ttggtttcac cgaactatgt tgtggaaaac cgctggatag
     1441 catcgggcta tctggtacag ccaatcatta cttggatgaa ttgcgcaatg agttagaaac
     1501 cagcggcgcc aggcaaatta tcactgcctg cccaaactgc gaaaacagcc tcagatccaa
     1561 actgccaggg ctcgatattc actcgatata tggattgatg ctcgatgccg gggtccatgt
     1621 gacgggcgag ggtacactta ctttccatga ctcgtgccca gatcgctacg gaagcagtaa
     1681 tccgcaggac gtgcgtcagc tcctatcagg attccaacaa gtcgagatga aaagccgggg
     1741 aagggatacc atttgttgtg gctctggcgg gattgtttcg atggtggatc cagaattatg
     1801 cgcctcgcgt gcccagaaaa gaatggcaga atttttttca accagggcgg atacctgcgt
     1861 gactgcgtgt atggcttgtt cacaccgctt agcccagaac attcgcgcag ggcaggtaag
     1921 gcactgcctg gaatatgtat tggatatcca ggtggactat gagcaggttg agaaaaatac
     1981 aattgcgatg tgggaggggc gtaccggaga aatcaatttg gcgcgcctgg ctcaagctca
     2041 tgaattgagc gctgaagaag agaaaagcgg tgaaaatgcc tgaaacgatt aatgttctgg
     2101 atcataccga aagtctttgc cccatctgcc ttaagattat tgaggccaga atagttgttc
     2161 aaaatggggc tgtacatatg gagaaggttt gccccgatca tggggcagcc aatgcttatc
     2221 tctggccaga tccagatcat tacctttgga tgaactcatt ccgcttgccg ttcacgcaac
     2281 cccaagcaga ggtcccactc caggatggct gcccactgga ctgtggatta tgtcccgcac
     2341 atctacggca tgcaaccctg gttgagatcg aagttaccca acgctgcaat ctacgctgtc
     2401 cggtgtgttt tatgtcagcc ggcgctgaat tacctgaccc cgatatgtat acgctaagag
     2461 ccatgcttga agcaacttat cgacaatcag gtgggcagac cagtgtgcag attaccggcg
     2521 gcgagccaac agtgcgaaaa gatctgcctg aaattattcg cctggtaaaa gaaaccggtt
     2581 tttctgctgt tgaagtgaac acgaatggtg tagcgattgg acgtgaccct gcttatatac
     2641 agactctggt ggatgccggg atcagtggaa tctacctgca atttgacggg ctgacccagg
     2701 cagtgtatac gcaaatccgg ggcgctgacc tgctgaacac caaactgcaa gcgattgaat
     2761 attgccgggc tgcaggtgtc caggtggtgt tggccatgac catcatttgg ggtgtcaacc
     2821 acaatcagat tggcgatgtg ctggattttg cgctgcgcaa tcaggatgtg attgttgggg
     2881 ttgccttcca gcccgcgttt acctcagggc gtttttctgt tcaaccagag cgccgcctga
     2941 ctatgggcga cgtgatattt atgctgtccg aacaaagcca gggactcatc gaaccttacg
     3001 acctttggcc gttgagttgc tcgcacccgc tgtgttcctg cgcgacgtat atcattgaag
     3061 accagggcac gcgaaaaccg tttacacgcc tgattacctc ccaagaatat atccaaggtt
     3121 ttgattccca cagcccccag ggttctgtgt ttgcagatat cgcggcgcga caatttccgg
     3181 ccatctcgca tggtttatcg gtggtcatta tgaattatat ggatgcgatg aatatagatc
     3241 tcaagcgcct gaaggaatgc agtatgaccg tcaccatgga aaatggccgg catatcccct
     3301 tctgcgccta ccagttgacc gatttgaatg ggaagcgttt gtatccgatg tgggggaggg
     3361 tagaaggcca gtctgtacgg gaggctggca atgcctgagg tcaaacgggt gcgggcgaac
     3421 tgccgtttct gtggttacct gtgtggactg attgccacgg ttgaagatgg gcgggtgatc
     3481 gcggttgagc cggaccccag ccgctaccca tacgatgcca gtgttcaacg tggctgccgg
     3541 cgctggagga ccaatctaga gttcctcgat caccccgacc ggatcaattt tcctctcaag
     3601 cggttgggag agcgtggcag tggacagtgg gagcaagttt cctgggaaga agcagtagat
     3661 gacattggcg cacgtttgca gctgctcaag gaccgtcacg gaccggagac gctggcaacc
     3721 agcattggcg gtccgcatgc cacctactgg cccttgcatc gcttcatgaa cctctttggc
     3781 agtccgaata acattgggat tgggcagatc tgctggaatc caggggtgtg gatcaacact
     3841 ctgacctacg gctggccact cgagaatgag ctggatccgc aaactacgga ttgcgctttt
     3901 ctatggggga cgaatccagc cgaatcagat aattccttgt tttggcgggc catactggag
     3961 taccgccata caggaaaacc cctgattgtc gttgatccca gaaagactaa aaccgcggcc
     4021 caagccacgc tatggctgcc tatcaatcca gggacagatg cttttctggc tttgggattg
     4081 ctgcaccaaa tcctcacgga aaaactatat gatcaccggt ttgttgaatc atggtgcagc
     4141 gggtttgagc acctcgtaga gcatgtagcc caatattcac ctgaatttgt tgccaagatt
     4201 accggggtaa acgcggagca gattgtcgag gccgcccggt tgtatgcgat gaatgcccca
     4261 gcaagcttga tcagtggtcg ggggatcgac caactcggcg cgaacagcat ccctactcac
     4321 caggcgctgg tgctcttgcg ggctgtcacc ggcaatgtgg atgtgccggg ggcttcgcac
     4381 ctgggagaga tgcctgcttt cacacccgag gtggatctgg agttgagtga tgaacttccg
     4441 gaaatccagc gccagaaagg gctcaaccgc cagctgctgc agtcctattc cgggtatgcg
     4501 cgtgtccgtc aggtgaccgg acgggctggg cggcgcttgc cgatgcgcta cctgacttcc
     4561 gcccatccca acctggtctg gcaggcgatg ctgaccggtg agccttatcc catccgtgcg
     4621 ctggtggtca tgggcagtaa cccgctgcta acccaggctg actcacggct gatttaccga
     4681 gttcttaaga gcctggactt gctggttacg ctcgagtact ttaaaacccc tacggctatg
     4741 ctggccgact acattctgcc tgctgccggt ggcctggagc gcccactgtt tcagacgcac
     4801 gccgggatcg ccaacattgc ctatggcggt gatcaggctg tagagccgta ttacgagcgg
     4861 cggactgatt tttacttctg gcgagaattg gggctgcgcc tgggtcaggc agaacgctgg
     4921 ccttgggaga ccttccaggc agccttggaa gccacattaa cccccgctgg tctgagctgg
     4981 gagcagttct gcgagagtgg attgtattac caacctggtc cttatcaaaa acacgaacgg
     5041 ctcgatccgc tcagcggtga aaaggaaggc ttcggcacac ccagtgggaa agtggagctc
     5101 tattcggaac tcttagccga attgggatat gctccattac cttcacctcc cccacctcga
     5161 ccgcccgagg gcagcttccc tctgctgttg acaactggag cccgaaagca gcctttttac
     5221 gcctcctcct accgccaggt ggtatccctg cgccagatgc atccagagcc atgggcggag
     5281 atgagcgaaa ccacggctgc caggcttgga ctggaagacg gctgtccaat aatcgtcgag
     5341 acggagcgcg ggcaggcccg cttcattgtg aaaatcgccg agatgcgccc cgacgtagtc
     5401 agcgtcgagt atggctggtg gttcccggat ctgcctgcct gcgagccaga cctgggaggg
     5461 gtttggattt cgaatgcaaa catgctgacc agcgctgaca tccggacctc agatccagtg
     5521 attggaacct ggacctataa cgggctgcct tgccgggttt tgcctggcgc ggatgagccg
     5581 gttaagcagg aaatggagat agttaatgag cggtaaaaaa cacgcattct tattgtttac
     5641 caaagcacct gcgccgggtg tgaccaaaac ccgccttacc gaagcgcgcg gcggtattct
     5701 caccccagaa gaggcagccg atttttatcg cgccgcccta ttagatgtgg ctgagctagc
     5761 ctttcaggca ctggctgagt tgaaccaggc agcccaaacg ggcgccaacg gcagtgaccc
     5821 tgacaggtat gatttcatcg taagtagtcc cccaaaaacc gatcaggcaa ttttggaagc
     5881 gctgttttcc gaaaacgagc cctggccagc gcctgtacag ttcattaacg accacggtaa
     5941 aactttcgac gagcacttcg acgatgcttt ccagcagctc tttgaccagg gctatcattc
     6001 ggtcgtctcg atcggcggtg atctgcctac aatgccggtc agccacatcg ttcaggcttt
     6061 tcagtggctc gagtactatg acacatactc tgaaaaaagt gggtttgtgc aggcaccttg
     6121 ccaggagtgc ggagtgtcgt tggtgggcta cacagccaaa acgccgatgg attcgaccgg
     6181 cgtattctac aacttagatg gtgtaccggc tttggatgcc tatatcgcca aagccgccca
     6241 gcgcggggta cctgtggcaa gcctcacgcc agtcgctgac gtggacgaca tgcgtgatat
     6301 ggcgcacact atttcgctgc tgcatgggat tgcctattcc agtcagttcc agcccggctt
     6361 gttcgttgcc cgacggaccc tggattggat ccagcgcacc aggattgtca tcaacacccc
     6421 gcccaatgcg gaacatgacc cacgggagcg catcgatgcc tgagattgaa ctcgctgaag
     6481 aacgctgcat tagctgcggc ctgtgcgttg atttctgccc ggtcaatgtt ttggagatga
     6541 ttgagatcga agggcgcctg ctcccggcgg cggtcagggt ggaggactgt tgggcttgcg
     6601 atacctgtgt agggcagtgc ccgactggtg cgctgcgggt agtggaagcc tacgagccga
     6661 ccctggtacc agctgcgaag caccctgggt gcaatggcca cgaaccggtt gctgcggggt
     6721 taactaccga gcagcaagct ctctaccagt cctggtctga aacgctgatg cgggtgctgc
     6781 gtctgcgctg gagcccggtg gcgatcaacc ttatcccggc cggggcgccg ttgcccgacg
     6841 cccctcaacc gcgggaaaaa ctgcgctact gccagtcgct gatggccgcc cggcgcggca
     6901 aaaccctgct catgccggcg cactgccatg cctgcccgga cggaacctcc atcctgggtc
     6961 tgaccgagat tccgcccaag ctggccagtg gtgagttata tatccgcttc ggtaagttgg
     7021 ccacaatcga agccgcccgc cagatggtgg ctgaacggcc gcgcctggag cctcgctcag
     7081 tccaggctac gatggttgcg cccctgcctc aggcgatctt accaccggac gttgtagcta
     7141 tcatcgctca accggaacag atgatgtggt tgtgcatggc ggcttcgttc tacaccgggc
     7201 ggcgctttga tttcaaggtc agtggctata acgcccagtg cgtcgagaca accctgttcc
     7261 cctataccag cggaaagatc aatatttccc tgggttgtta cggctgccgc gccagctcgg
     7321 atattggcga tgatctgatg ttcatgggcg taccggtaga ccagatgccc accttgatcc
     7381 aggggttgaa ggagctgggg aagaaagcca tccccgattc tcgcaacaaa atctacctgg
     7441 agccgctgac atgagcaggc tagttcttca accggaccgg gatctgaggc gaaaagaaat
     7501 ctgcgggagt gaaattgctg ccttgctgaa ccccaggctg gcagtactgg tcagttgctg
     7561 tgacctcacg ggcaagccta atatccttag cgtcgcctgg cactcgccgc tctcccacaa
     7621 gccgcccctg gtagggatcg ccatccaccc caggcgctac tcgcacgatt tgatccgggc
     7681 tgggcaggag tttatcgtta atgtggtcgg aatggctttt gaagacgcgg tgcagatttg
     7741 cgggtcatac agcggcgcct ccaccgataa agcgaaactg gctggtctga gcttctctcc
     7801 cgcccggact gtccggcctc cctaccttac caatgcctta gggcatctag aatgccgggt
     7861 gtggggggag gtgccagttg gcgaccatac cctgtttatt ggacaggtgc agtttgccga
     7921 agcccttgaa gcctgttttg acgcctgctg ggatccgtcc aaagcagacg tactgctctg
     7981 cttgcaacgc gatcggctgg tcaccttggg tggaggaaag tcgctccccc aagggtccgg
     8041 aagcgcagcg gaggatgcca cctgggatct tgaggagttg atatgccaac cgtaacgtca
     8101 aaatatgggg atgaagaggc tctggaggcg ctggtaccat cctttgagga ccgtctgggt
     8161 caggagaccg cttccctggt ccggcatagc ctggatagtc tgcagatcaa cgtgggcaaa
     8221 cagtgcaacc aggcctgcct gcattgccat gtggaggcag gcccgcagcg ggttgagatc
     8281 atggaccagc gaacggctga actggcgatc gagtttgcct gcctctcagg agcccagacg
     8341 gtggagatca ccggaggcgc tcctgagctc aacccttcct tccgatacat ggtaggccag
     8401 ttgcgccagg ctggactgcg ggtgatcgat cgctcaaacc tgaccgtgtt tttcgagcca
     8461 ggccaggagg agctgcccga attcttggca gggatgaagg tcgaactagt ggccagcctg
     8521 ccctgcttta ctgagaaaaa cgtggaccag cagcggggtc gcggggtcta tcgcaaatcg
     8581 gcgcaggcgc tgttgaagtt gaacgagctg gggtatggca aggaggattc cgggctggat
     8641 ctcaacctgg tgtacaaccc actgggcgcc tacctgccgg ctcctcaggc agagctggag
     8701 gctgattaca agcgtgaact tgctacccgc ttcgggatcc gattcaacca cctttacacc
     8761 attaccaaca tcccgatcgg gcgctttgct cacatactgg ccaggcaggg cgagatgcag
     8821 gcttatgccc gcctgttggt cgaatcgttc aacccggcca cgctggacaa cctgatgtgt
     8881 ctgcgtcagg tcagtctctc ctgggatggg ttaatttacg actgcgactt caatcaaatg
     8941 ctggatatgc gtttgagtaa cgggcgcgtt ttccggctgg gtaaagcgcc cgccgcacag
     9001 atcgccgcct ccctgatagg taagcccatc cgtaccgggg ctcactgcta tggatgtacg
     9061 gccggattcg gcagttcgtg cgcaggggcg ctggcctgag ctgtggcgag atgccatgaa
     9121 agacccctgg gtagatgtta aggaagcgat gacgataatg agctgggaag agtcagccga
     9181 tagtggaatg gaaggcgcgg tagccgggtc agggaacctc tctcgacctt ggctgcggct
     9241 cgggcgtccg gaggatgcgc cagctttaca gcaactgata gccgaggccg gtatgtatgc
     9301 tgaagtggac ccgttggagt gcctggtagc cgacacgcct catggggtca ttgggttcgc
     9361 ccgggtggag ttctttgaag atgttcccta cctgcgtccg gtggttgttg cacctccata
     9421 tcaagggcac ggagttggcc ggcgcctggt agaaggctta ctggccgatt atccagggct
     9481 atacgtggtg tctcgcggga aagcagccgg cttctaccag cgcctgggat ttcaagccct
     9541 accctggtcg gctgtggcgg ctgaccatca agaggaatgt gatctctgtc cggagcgcag
     9601 ccagtgttgt ccggtgggct tgcacgccgg acggaacgaa gagtgttgat ggtaagtgtg
     9661 cgtttggttg gctatcctga tgtacaggcc gccgggtgaa acgacacaac gaatc
//
LOCUS       DATMXK010000121         2442 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51924, whole genome shotgun sequence.
ACCESSION   DATMXK010000121 DATMXK010000000
VERSION     DATMXK010000121.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2442)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2442)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2442
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51924"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1200
                     /gene="rny"
                     /locus_tag="VN363_02240"
     CDS             <1..1200
                     /gene="rny"
                     /locus_tag="VN363_02240"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560016.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004521 - RNA endonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease Y"
                     /protein_id="HWQ83354.1"
                     /translation="RQSALDKRANDIENLHAEEVAELQRISQLTGEEAREILLAEVEK
                     EARSDMARIIRQIETEAKEEGENRARKLITAAIQRVASEHVAEVTTSVVALPSDDMKG
                     RIIGRNGRNIHAFEQAAGVDVIVDDTPEAVTISCFDTVRREVARRALGKLILDGRIHP
                     AHIEKILKNEQKEVERDMQEAGEEAVYEAGVSALHPEIIKMLGRLKYRTSYGQNQLFH
                     AVETAKIATVLAAELGADVEVARMGGLLHDLGKAMDHNMEGTHASLGAEFAKRYAVPA
                     KVVNTIASHHHEVEQETLEAAIVEAADAISGARPGARRESLEQYIKRVKALEEIADSH
                     KGVTQSYALQAGREIRIFVRPEDIDDLACIRLARDVAHKIEETMQYPGQIKVTVIREN
                     RAVDWAK"
     gene            1365..>2442
                     /locus_tag="VN363_02245"
     CDS             1365..>2442
                     /locus_tag="VN363_02245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWQ83355.1"
                     /translation="MLPWLAEGIALLGAILYIAQTVVYTFTQDTILDEGAYLLKGYLF
                     TTGQYTIFQDNGAWSNHMPLAFLIPGVVQVLFGPGLRAARYFAVFISILTLLGVWLVA
                     RRSGGRWWAAIAVWATAWNPALVKMYSVALSQGLIACMFIWSVYFVLGEKRSLWQIGL
                     GAALAGAMFMTRINMAFVLPLLILYVLWQYGARAASVALLSGGLVVGIGHAVYWPNIL
                     RLWAYWLPESLTPFLNAYRLTAESGIRFWDPDLSLSSRVVSFFHSFRFQFISLVGFIG
                     SWLLWPKRSSWKKDSDFRSAVYLSSLFAGLWLAHLWATMGNEYCVYCLAGYIAFFISA
                     ALIVVILSAAIWSKEMGGLRQLGIG"
BASE COUNT          552 a          593 c          691 g          606 t
ORIGIN      
        1 cgccagagcg ctctggataa acgggcaaat gatatcgaaa atttacatgc cgaagaagtc
       61 gctgagctgc aacgcatttc tcagctgacc ggagaagagg cacgcgaaat cctgcttgcc
      121 gaagttgaaa aggaagctcg ctcggatatg gcccgcatca tccgccagat tgagacagag
      181 gcaaaagaag aaggggaaaa ccgggctcgc aagttaatta ctgcggcgat tcaacgggta
      241 gcctccgagc atgtggcaga ggtgaccact tcagtagtcg ctttgccatc ggatgatatg
      301 aagggccgga tcattggtcg caatggacgg aatattcatg cctttgaaca agccgcaggc
      361 gtagatgtga ttgtagatga tacacctgaa gcagtcacaa tctcctgttt cgatacagtg
      421 cggcgagaag tagctcgccg ggcgctcgga aagctgatcc tggatggacg gatccatcct
      481 gcgcacattg agaaaattct caagaacgag caaaaggaag ttgaacgaga tatgcaggaa
      541 gcgggtgagg aggcagtgta tgaagccggc gtctctgctc tccatccaga aatcatcaag
      601 atgttgggcc ggttaaagta ccgcacctcc tatgggcaga accagctctt ccatgctgtc
      661 gaaacagcca agattgcgac cgttctggcc gctgagcttg gcgcagatgt tgaggtagcc
      721 aggatgggtg gcctcttaca tgacctgggg aaggcgatgg accataatat ggagggtacc
      781 cacgccagcc tgggcgcaga atttgccaaa cgttatgctg ttccggcgaa agttgtgaac
      841 accatcgcct cgcatcacca tgaggttgag caagaaaccc ttgaagcagc gatcgtcgaa
      901 gccgcggatg caatctcagg cgctcgccct ggtgctcgcc gggagagcct ggagcagtat
      961 atcaagcggg tgaaagcgct tgaggaaatt gcagactcgc ataagggtgt aacgcagagc
     1021 tacgccttgc aagccgggcg cgaaatccgc atctttgtcc gtcctgaaga catcgatgat
     1081 ctcgcctgta tccggttggc tcgggatgtg gcacacaaga tcgaagaaac gatgcagtat
     1141 cccggtcaaa ttaaggttac cgtcattcgt gagaatcggg cggtagattg ggctaaataa
     1201 gcatttttgc tgtgggtcaa ctatccagca tctggctaaa tgaacactga ctgctccttg
     1261 gaggaaacaa gattgtcacc aactcgatct tgccactcta aggagcagtt tttcgtataa
     1321 ttcaattgat accttatatc gatagcttcc gtagacgatt gagtatgctt ccctggctgg
     1381 cagaagggat tgccttgctg ggtgcaatcc tgtatatagc ccaaacagtc gtctatacct
     1441 tcacacagga tactatcctg gacgaaggcg cttacctgct caaaggctac cttttcacca
     1501 ccggtcaata tacgattttt caagataacg gcgcgtggag caaccacatg ccgctggcat
     1561 ttttgatccc gggagtcgtt caggttcttt ttggtccggg attacgcgct gctcgctatt
     1621 ttgcggtctt catcagtata ctcacactgc ttggtgtgtg gctcgtagcc cgccggagtg
     1681 gggggcgctg gtgggctgcc atagccgtct gggcaacagc ctggaatcca gcgctggtga
     1741 agatgtacag cgtcgcatta tcccagggct tgattgcctg catgttcatc tggtcggtct
     1801 acttcgtgct tggtgagaaa cgctccctct ggcaaatcgg actgggagcc gccctggcag
     1861 gagccatgtt catgacccgc atcaatatgg catttgttct tcccctgctc atcctgtatg
     1921 ttctctggca gtatggcgcc agggcggcca gtgttgccct gctcagcggc ggactggtgg
     1981 ttggaattgg acatgcggtt tattggccga atatcctgcg tttatgggct tactggctgc
     2041 cagaatcctt aacacccttc ttgaatgcct accggttgac agcagaatcc gggattcgtt
     2101 tttgggatcc tgatctctct ctttccagca gggtggtgag tttttttcat agtttccggt
     2161 ttcaattcat ctctctggtt ggttttatag gatcatggct gctgtggccg aagcggagtt
     2221 cctggaaaaa ggatagcgat ttccgcagcg cggtctattt atcgagctta tttgctggct
     2281 tatggttggc gcatctatgg gctacgatgg gcaatgagta ttgcgtctac tgcctggcag
     2341 gctatatcgc ttttttcatt tcggccgcct tgatagtggt gatcctttca gctgctatat
     2401 ggagcaaaga gatgggcggg ctccgccagc tcgggattgg tc
//
LOCUS       DATMXK010000122         2475 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_25888, whole genome shotgun sequence.
ACCESSION   DATMXK010000122 DATMXK010000000
VERSION     DATMXK010000122.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2475)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2475)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2475
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25888"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1954
                     /locus_tag="VN363_02250"
     CDS             <1..1954
                     /locus_tag="VN363_02250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HWQ83356.1"
                     /translation="DLGIFGMWAHAPWVHWLMFALATPVQFYVGSQYYVGAYKALRNG
                     SANMDVLIAMGSSAAYFYSIVVMLNIIPGHVYFETSAVIITLIKLGKYLEARAKGRTS
                     EALKKLMGLQPKQAHILRAGQEMEVAVDEVRVGDIVLVRPGEKIPVDGVVTEGRSAVD
                     EAMLTGESLPVEKGPGAAVIGGTLNKLGLLRFEATKVGRETALAQIIKLVEDAQGSKA
                     PIQKLADQVSAVFVPVVIGIATLTFLVWLLLIPLPAGAETNLFTRALINMVAVLVIAC
                     PCALGLATPTAVMVGTGRAAELGVLFKSGEALERLGSVTTVVLDKTGTITRGQPAVTD
                     IVLAHQPVQVSAGLAVGPDGREINPDGHSPRRLDENELLRLAASVEKGSEHPLGEALL
                     AEAGERSLVLAEPQGFLTEVGHGVRAEVDGRAVWVGNLRMLQAAQIFLNGLEPEIDRL
                     QNEAKTAILVAIDGSLQGVIGVADTVKAGSLEAIASLHALGLEVVMITGDNQQTADAI
                     ARQVGVDRVLAEVLPADKANQVKALQGQGKVVAMVGDGINDAPALAQAEVGIAIGTGT
                     DVAMAAAPVVLISGDLRGVERALKLSRRTLKTIKQNLFWAFVYNIILIPAAAIGLLNP
                     MLAAAAMASSSVFVVSNSLRLKRVRL"
     gene            complement(2007..>2475)
                     /locus_tag="VN363_02255"
     CDS             complement(2007..>2475)
                     /locus_tag="VN363_02255"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ83357.1"
                     /translation="DAGEINLGPSVKIGHYAQEHETLDPDETPLEAVRCASTLSESHA
                     ISFLIRYLFTYLQASQKIADLSGGERSRLQLALLVLSGANFLLLDEPTNNLDVASAEV
                     LENALEDFEGTVLVISHDRYFLDRTVNRILLLEDGRIKEFAGGYSDYAAAVRI"
BASE COUNT          449 a          733 c          803 g          490 t
ORIGIN      
        1 tgacctgggc attttcggta tgtgggcgca cgccccctgg gttcactggc tgatgtttgc
       61 cctggcgacc ccggtccagt tctacgtcgg cagccagtat tatgtaggcg cctacaaggc
      121 cctgcgcaac ggctcagcca atatggatgt gctgattgcg atgggttctt cggcggcgta
      181 tttctattcg atcgtagtca tgctcaatat tattcccggg catgtctatt ttgagacctc
      241 ggcggtgatc atcaccctga tcaagctggg caaatacctg gaggcgcggg ccaaaggccg
      301 caccagcgaa gcgcttaaga agctgatggg cttgcagccc aaacaggcgc atatcctgcg
      361 cgccgggcag gagatggaag tagctgtgga cgaggtgcgc gtcggcgata tcgtcctggt
      421 gcgccccggg gaaaaaatcc cggtggatgg ggtggtaacc gaaggccgct cggccgtgga
      481 tgaggcgatg ctgaccggtg agtcgctgcc ggtggagaaa ggcccggggg cggcagtgat
      541 cggcggtaca ctcaacaagc tgggcctgct gcgctttgag gccaccaaag tcgggcgcga
      601 aaccgccctg gcgcagatta tcaagctggt ggaggatgcc cagggttcca aggctcccat
      661 tcagaagctg gcggatcaag tctctgcggt cttcgtcccg gtggttatcg ggattgccac
      721 gctcacattc ctggtctggc tgctgctgat cccgctgccg gcaggcgctg agacgaacct
      781 attcacgcgc gccctgatca atatggtcgc cgtgctggtg attgcctgcc cctgcgcatt
      841 gggtctggcg acccctaccg ctgtgatggt aggcaccggt cgggcagctg agctgggcgt
      901 gctgttcaag tccggcgagg cgctcgaacg cctgggcagc gtgaccaccg tggtgctgga
      961 taagaccggc acgatcaccc gcggccagcc ggcggtgacc gatatcgtcc tggcccacca
     1021 gccagtgcag gtatcggcgg gtttagccgt tggcccggat ggccgcgaga tcaatccgga
     1081 tggtcattcg cccagacgcc tggatgaaaa cgaattgctc cgcctggctg cctccgtgga
     1141 aaaaggctcc gaacaccccc tgggcgaggc gctgctggca gaagccggcg agcgcagcct
     1201 ggtgcttgca gaaccccagg gctttcttac ggaggtaggc cacggcgtgc gcgccgaagt
     1261 ggacgggcgc gccgtgtggg tgggaaacct gcgcatgctt caggccgctc agatcttcct
     1321 caacggcctg gaacctgaga tcgaccgctt gcagaatgaa gccaaaactg ccatcctcgt
     1381 tgccatcgac ggcagcctgc agggtgtgat cggcgttgcc gatacggtca aagcaggctc
     1441 gctagaagcc atcgccagcc tgcacgcatt gggtctggaa gtggtgatga ttaccggcga
     1501 caaccagcag accgccgacg ccatcgcccg ccaggttggc gtggatcgcg tcctggcgga
     1561 ggtgctgccc gccgataagg ctaaccaggt caaggcgctg caggggcagg gcaaggtggt
     1621 agccatggtc ggggatggga ttaacgatgc tcccgccctg gctcaggctg aagttggcat
     1681 cgccatcggc actggcaccg acgtggccat ggcagctgcc ccggtcgtcc tgatcagcgg
     1741 tgacctgcgc ggcgtcgaac gcgctctgaa gctctctcgc cgcactttga agaccatcaa
     1801 gcagaatctg ttctgggcgt ttgtctacaa catcatcctg atccccgccg ccgccattgg
     1861 cttgctcaac cccatgctgg cagctgccgc catggcttcg tcaagcgtgt ttgtggtcag
     1921 caacagcctg aggttgaagc gggtgaggct gtaggggaca agttattaat agcaaatcgc
     1981 ctgcgatctt cgcaggcgat ttgctgtcaa attcttactg ccgcggcgta atcgctgtag
     2041 ccgcccgcaa attccttgat ccgcccatct tccagcagca ggatccggtt taccgtccgg
     2101 tccaggaagt agcgatcgtg cgagatcacc agcaccgtgc cttcgaaatc ctccagcgcg
     2161 ttttcgagca cctcagccga ggcgacatcc aggttgttgg tcggctcgtc cagcagcagg
     2221 aagttggcgc ctgaaagcac aagcaaggcc agctgcagcc ggctgcgctc cccgcccgag
     2281 aggtcggcga tcttctggct tgcctgtagg taagtgaaca ggtagcggat caggaacgag
     2341 attgcatgcg attcgctcag tgtgctggcg cagcggactg cctccagggg agtttcatcc
     2401 gggtcgaggg tctcgtgctc ctgggcgtaa tgcccgatct tgacgctggg tccgagattg
     2461 atctcgcccg cgtcg
//
LOCUS       DATMXK010000123         8642 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_71709, whole genome shotgun sequence.
ACCESSION   DATMXK010000123 DATMXK010000000
VERSION     DATMXK010000123.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8642)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8642)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8642
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71709"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2050)
                     /locus_tag="VN363_02260"
     CDS             complement(<1..2050)
                     /locus_tag="VN363_02260"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560875.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 65 protein"
                     /protein_id="HWQ83358.1"
                     /translation="MHDLWTITESQFDPLRQQHSETVFTLGNGYLGTRGAFEEFYPGE
                     ERTTFVHGIFDDIPIVFTELVNFPDWLELEIILSGERFNLAEGEILDFNRQLNLFTGL
                     LTRTVRWRSPKGKTTRLEFRRFASLADEHLACLQVLIIPEDYSGSLALRSGLDAATNN
                     QGYRHWDWLDQGLAGNTCWLKLRTKTTAIEAGNAARLTLFADGIVTTHAWDVHGHPTL
                     ACETSLRAGQAVRLEKFVSIYTSRDRPDPLASARAALAGLPASAWDELWQAHTRKWEE
                     EWRRCDVIIEGDAEAQLAIRFNLYHLLIAAPRSDERVNIGAKTLSGHGYRGHAFWDTE
                     IFMLPFFTFTRPQIARSLLSYRFHNLPGAVEKAASGGFSGAQYPWESAGDGLEVTPTW
                     FPNPEDRANLIRIWTGDIELHISADIAFAVWQYWLVSQDHEFLSQRGAEIILETARFW
                     VSRLEWDSERGLYEITDVIGPDEYHEHVDNNAYTNYLVRWHLRKAVWLAGWLVDYYSP
                     AARVLFGRLGMDEETFSRWRQIAEQIYCPTPASSGLIEQFDGYFERTDLSLASLEPRS
                     SSVQSLLGIEGTNETQLLKQPDVVMLMYLLPEEFDAAAAQANFDYYTARTDLTYGSSL
                     GPSIQAILATRHGSLDSAYENFLRAARADLEDVRGNAADGIHGASAGGLWQAVVFGFA
                     G"
     gene            complement(2093..3013)
                     /locus_tag="VN363_02265"
     CDS             complement(2093..3013)
                     /locus_tag="VN363_02265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560288.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosidase"
                     /protein_id="HWQ83359.1"
                     /translation="MQLQRHPANPILLPDPASDWETYNVLNPSVIYHAGLFHMHYRAQ
                     GLDWISRIGYAVSPDGVHWNRMRRPVLQPQDAWDDRGIEDPRLTEIEGMFYMAYTAYG
                     SQPGIPGAATHAGGGVLPMIARSSNLITWERLGPIVTGEDNKDHVLFPRRIQGRYVAF
                     HRRPPQVWLAESEDLIHWPEEYMRPIFGPRAENGWDSFRVGGGGVPVATEHGWLAIYH
                     AYDPAHTYRLGVCLLDLENPAIVLHRPEGFVLEPRELWELRGDVPNVVFSCANPVVGD
                     KVYVFYGGADHVIGLATIGFDELLEYARFG"
     gene            complement(3076..3711)
                     /locus_tag="VN363_02270"
     CDS             complement(3076..3711)
                     /locus_tag="VN363_02270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83360.1"
                     /translation="MLRELVRILLAALQDIWADFWTTLAVNLICLLAWLFIVPGPPAA
                     LALTYYANRLAHGEVADLADFWQAFRRYWAPAWRWGSLHLGVVAFLGADIFLTGQFTQ
                     GLWKTYLQGLYLALLAAWLALQIFVLPFLFEQQTLSVRQALRNAAALVGRNPGFSLGL
                     ICLITLVLIAGTLTFLLSLMLGAVFLACAGNRAVLNRLEAFKLSNPSGSAG"
     gene            complement(3743..4645)
                     /locus_tag="VN363_02275"
     CDS             complement(3743..4645)
                     /locus_tag="VN363_02275"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008643 - carbohydrate transport [Evidence
                     IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate ABC transporter permease"
                     /protein_id="HWQ83361.1"
                     /translation="MITTEQKAVQPKVTQPVVSTQRILNIISTILRYVLMIAIGLIMF
                     TPFIMAMIGTFKTDLEITAFPPKFFPEVWHTENWTRVWNTDLGQGGTFPRWLFNTAFL
                     AVTVSVSQVIFGSMAAYAFARLKFAGQEIVFAFMLATMMIPGAVTLIPAYVLMTKLGL
                     INTFWSLILPGLVGAGSIFTLTQFFKSIPKDLEEAAVIDGATHMQIFRIVILPLARPA
                     ILTVLILQFQGMWNAFLQPLLYLNSPDKYVLNVALSIFQQQFSAQWNLTLVGAMFNAI
                     PVIILFFIFSRYYIEGVAYTGIKG"
     gene            complement(4656..5855)
                     /locus_tag="VN363_02280"
     CDS             complement(4656..5855)
                     /locus_tag="VN363_02280"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWQ83362.1"
                     /translation="MLVFIGIVAAILIIALLNAPTMGGPRVMASLQTYLAEVRATAIP
                     YILAVGLLGVVVAFFAAREVRRVWPNVRQRTELLTAYAFLAPYLLVTLTFTIGVVLFA
                     FYISFTNYDIFTRPTWAGLENYAAAFEGFTDPTRRDFLQALYNVLWYSFIVVPIQTGL
                     AILLAVLLNAPMKAKQFFRTIFYAPSVTSSVVITLIFVWFYLRNGYINYFLQSALGWI
                     GVDFTPINWLGDPRGLIQLILEPFGVDIPSSQWYFRGPSIAWMAIMFQNIFTTVPTFM
                     IMFLAALQDINPTLYEAAKIDGANGWQAFRRITLPMLRPIILLVIVLGTIGTLQIFDQ
                     VYLATKGGPLNTSLTPVFLIFQEALGVNGPITMGYATALAFILAVIIFVFTFIQRRFI
                     ESGTELY"
     gene            complement(6071..7393)
                     /locus_tag="VN363_02285"
     CDS             complement(6071..7393)
                     /locus_tag="VN363_02285"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter substrate-binding protein"
                     /protein_id="HWQ83363.1"
                     /translation="MRKSLTLFTVLIALSMLIAACGTPAATTAPAAETQAPPADATAP
                     PAASGTCPLPVEAGATISFSGWGDETEQQVYRDTIERFKGVCPDVTVNYIPVPSDFQT
                     KLKAQMAGGTAPDVFYVDDQLMTAFAPSEQIMPLDDLMAQVGITRDDFIPALLTIFTL
                     DGKTYALPKDWGTLGLIYLPEAFADAGIPEPTADWTWEDMRAAAKTIADKGTFGGFCM
                     GADWARFAPFVTSNGGSFASPDFTTATLDTPEVKTMATFVNDMFQEGSLVRAADVSAG
                     WCGEAIGKKAVAMTTEGGWMVNFMKTTYPEVEWKAVEIPSGPVTRADIIFTNGIGINA
                     ATPFPNAAAAFLFYATGPENQAEIVKTGFAYSTHPEQADLIVDPNDMAIAQGGLLPDA
                     IVAYWGPNTGKVNDAVSQALDRIFLGDQTVDEAFAQAQAEAQTALDGG"
     gene            complement(7588..>8642)
                     /locus_tag="VN363_02290"
     CDS             complement(7588..>8642)
                     /locus_tag="VN363_02290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560874.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="LacI family DNA-binding transcriptional
                     regulator"
                     /protein_id="HWQ83364.1"
                     /translation="SKGVSMRKRVTIKEVAARAAVSYQTVSKVINHQANFSASTQQRV
                     WDAVHELGYTPSYTARSLKLQSSKTIGYSWQPSPPDHTNPILDEFLQSMFRTAESCGY
                     YLLCFPYHADPIEYLATYRDLIETGRVDAFVLSIVNYDDPCINYLLQRNFPFVAFGRL
                     DNPLSFPNIDIDGELGLSLAVEHLLSLGHRKIAALAWPTDSRVGNHRMSGYFSAMQKA
                     GIEAPEEWVQRGEGSYEVGYQCTLRLLEMPDSTRPSAIVAMNDSMAIGAMAAIRQQGF
                     QPGKDIAITGFDDTPIARYLNPPLTSLHQPVSQVGQNLMERLIAYLETGVFPDPFYEL
                     VAPQLMIRESTTGIEA"
BASE COUNT         1999 a         2303 c         2630 g         1710 t
ORIGIN      
        1 ggcctgcaaa gccaaacacc accgcctgcc acaaaccgcc tgccgaggcg ccgtgaatcc
       61 catccgcggc gttgccgcgc acatcctcca ggtcggcgcg ggcggcgcgc aggaagttct
      121 cgtaggcgct gtccaggctg ccatggcggg ttgccaggat ggcttgaatg gacggaccta
      181 atgaagaacc gtaggtcagg tccgtgcgcg cggtatagta gtcgaagttg gcctgggcgg
      241 ctgcggcgtc gaattcttcc ggcaggagat acattaacat caccacatcc ggctgcttga
      301 ggagctgggt ctcattggtg ccttcgatcc cgagcaggct ctggaccgag ctggaacgcg
      361 gctcgagcga agccaggctg aggtccgtgc gctcaaagta gccatcgaac tgttcgatca
      421 ggccggagct cgcgggggtg gggcagtaaa tctgctcggc gatctgccgc caacgggaga
      481 aggtctcctc atccatgccc aggcggccga acaggacccg ggctgccggc gaatagtaat
      541 caaccaacca gcccgccagc cagacggctt tgcggagatg ccagcgcacg agatagttcg
      601 tgtaggcatt attatctacg tgctcgtggt attcgtccgg gccaatcaca tccgtgatct
      661 catacaaccc gcgctcgctg tcccattcca accggctgac ccagaaacga gccgtttcca
      721 ggatgatctc tgcgccgcgc tggctgagaa attcgtggtc ctggctgacc agccagtact
      781 gccagacggc aaaggcgata tcggctgaaa tatgcagctc gatatccccg gtccagatgc
      841 ggatcagatt tgcccggtct tcgggattgg ggaaccaggt gggggtaact tccagtccat
      901 ctccggcgct ttcccagggg tactgagcgc cgctgaagcc gccggaagcg gctttttcaa
      961 cggcgccggg cagattatga aagcgatacg agagcaggct gcgggctatc tggggacggg
     1021 tgaaggtaaa gaacggcagc atgaatattt ctgtgtccca aaaggcatgc ccgcggtaac
     1081 cgtggccgct cagggtttta gcgccgatgt ttacacgttc gtcgctgcgg ggcgcagcaa
     1141 tcagcaggtg gtacagatta aagcggatgg cgagctgagc ttcagcgtcg ccttcaatga
     1201 tcacatcgca gcgccgccat tcttcttccc atttgcgggt gtgagcctgc cacaattcat
     1261 cccaggcgct tgcaggcagg ccggcgaggg cggcgcgagc agaggcgagc ggatctggac
     1321 ggtcccggct ggtgtaaatc gagacgaatt tttccaggcg aacggcttgc ccggcgcgca
     1381 agctcgtttc acaggccagg gtgggatgcc catgtacatc ccaggcatga gtggtgacga
     1441 tgccatccgc gaacagggtg aggcgggctg catttccggc ttcgatggcg gttgttttgg
     1501 tgcgcagctt cagccagcag gtattaccgg cgaggccctg atccaaccag tcccaatgcc
     1561 ggtaaccctg gttgttggtc gccgcatcca gcccggaacg cagggcgagg cttccggagt
     1621 aatcttcggg gattatgagg acctgcaggc aggcgagatg ttcatctgcc agactggcga
     1681 agcgccggaa ctccaggcgg gtggtcttcc ctttggggct gcgccagcgc acggtgcggg
     1741 tcagcagccc ggtaaataga ttaagctggc gattgaagtc caggatttcg ccttcagcca
     1801 ggttgaagcg ctcgccgctg aggataattt ccagctccag ccaatctggg aaattgacca
     1861 gctcggtaaa cacgatggga atgtcatcaa aaatcccatg gacaaaggtg gtgcgctctt
     1921 cgcccgggta gaactcctcg aaggcgccgc gcgtacccag gtagccgttg cccagcgtaa
     1981 aaaccgtttc gctgtgctgc tgccggaggg gatcgaattg ggattcggtg atggtccaaa
     2041 ggtcatgcat acatgtgcct tttccggtgg gaatgttatc gggttaagta gattagccga
     2101 acctggcata ctccaggagc tcatcaaagc caatggtcgc cagcccgatc acatggtctg
     2161 cgccgccgta gaagacatag accttatccc caacgaccgg attggcgcag gagaacacca
     2221 cattggggac atcgccgcgc agctcccaca gctcgcgcgg ctccaggacg aaaccttcgg
     2281 ggcggtggag cacgattgca ggattctcga gatcgagaag gcatactccc aggcgatagg
     2341 tatgcgcagg atcgtaggca tggtagattg ccagccagcc gtgctcggtg gcgacgggca
     2401 cgccgccgcc gcccacccgg aatgaatccc agccgttttc cgcccgcggg ccaaagatgg
     2461 ggcgcatata ttcttcgggc cagtggatca gatcctcgga ttcagccagc cagacctgag
     2521 gcggccggcg gtggaaggcc acatacctgc cctgaatgcg gcgtggaaat aagacatggt
     2581 ccttgttatc ctcgccggtc acgatcggac cgaggcgctc ccaggtgatc aggttcgagc
     2641 tgcgggcgat catcggcaaa acgccgccgc cagcgtgggt tgccgcccca gggataccag
     2701 gctggctgcc atatgcggta taagccatgt aaaacatgcc ttcgatttcc gtcaggcgcg
     2761 gatcttcgat accgcggtca tcccaggcat cctggggctg cagcacgggg cggcgcatgc
     2821 gattccaatg gacgccgtcc gggctgacgg catagccaat ccggctgatc caatccagtc
     2881 cctgggcgcg gtagtgcatg tggaacaatc cggcgtggta gatcacggaa gggttgagga
     2941 cattgtaagt ttcccaatcc gacgccgggt ctgggagcag aatgggattg gcggggtggc
     3001 gctgcagctg catggtgatc tccaaggggt aagggtcaat aaaccaggac gatatcgcag
     3061 gattggttcg cccgctcagc cagctgaccc ggaggggtta gaaagtttaa aggcttccag
     3121 gcggttcaaa actgcccgat tgccggcgca ggccaggaaa acagccccca acatcaggct
     3181 gagtaaaaag gttagcgtgc cggcaatcag gaccagggtg atcaggcaga tcaaacccag
     3241 ggagaagcca ggatttctgc caaccagtgc agccgcattg cgcagcgcct ggcgaacgct
     3301 cagcgtctgc tgttcgaaca gaaacggcag cacaaaaatc tggagggcaa gccaggctgc
     3361 gagcagcgcc aggtagagac cttgcaaata ggttttccat aagccctgcg tgaactgacc
     3421 ggtcagaaag atatctgccc ctaaaaacgc aaccacgcca agatggaggc tgccccagcg
     3481 ccaggctggc gcccaataac ggcggaaggc ctgccagaaa tcggccaggt ctgctacttc
     3541 gccgtgagcc aggcggttgg cataataagt cagcgccaga gcagccggcg gaccgggcac
     3601 gatgaacagc caggcaagca ggcagatcag gttgactgcc agggtggtcc aaaaatctgc
     3661 ccagatgtcc tggagtgcag ccagcaggat gcgtacgagt tctctcagca tgagtctgat
     3721 ggcgcgaaca gggtgctccc gcttatccct tgatcccggt ataggcaacg ccttcgatat
     3781 agtacctgct gaagatgaaa aacaggatta tgaccgggat ggcattgaac atggctccta
     3841 ccagggtcag gttccattgg gcactgaact gctgctgaaa aatgctcaag gccacgttga
     3901 gcacgtattt gtccggcgaa ttcagataca gcaaaggctg cagaaaggcg ttccacatcc
     3961 cctgaaactg gaggatcagc accgtgagga ttgccgggcg ggccagcggc aagatcacga
     4021 tccggaaaat ttgcatatga gtggcgccgt caatcacggc agcttcttcc aggtctttgg
     4081 ggatagactt gaagaactgg gtcagggtga aaatgctgcc ggcgccgacc aggccgggca
     4141 agatcaatga ccagaaggta ttgatgaggc ccagcttggt catcagaaca taagccggga
     4201 tcaaggtgac ggcgcccggg atcatcatcg tggccagcat gaaggcaaat acgatttcct
     4261 ggccagcaaa tttcaacctg gcaaatgcat aagcggccat ggatccgaag ataacctgag
     4321 acacggagac agtaacagcc agaaaagccg tattgaacaa ccagcggggg aaggtgcccc
     4381 cctgacccag gtcggtattc cagaccctgg tccaattttc tgtgtgccat acttcgggga
     4441 agaacttagg cgggaaagcc gtgatttcaa ggtcggtttt aaaggtcccg atcattgcca
     4501 tgataaaggg cgtaaacatg atcaggccga tggcgatcat gaggacatac cgcaaaatgg
     4561 tcgaaataat attcaagatt ctctgggtgg aaacgaccgg ctgggttacc tttggctgga
     4621 ccgctttttg ttcagttgtg atcatgacag gcctcctagt acagttccgt gccagattcg
     4681 atgaaacggc gctggatgaa ggtaaataca aaaatgatca cagccaggat aaacgccaga
     4741 gctgtggcat aacccatggt gattggccca ttgacaccca gggcttcctg gaaaattaag
     4801 aacaccgggg tcagggaggt attcaacggg ccgcctttgg tcgccagata aacctggtcg
     4861 aagatttgca aggtcccgat ggtgcccagg acgatcacca acaggataat gggtctcaac
     4921 atgggcaggg tgatccggcg aaaggcttgc cagccattgg cgccatcgat cttggctgcc
     4981 tcatataagg tcgggttgat atcctgcaaa gctgccagaa acatgatcat gaaggtgggg
     5041 acggtggtaa agatattctg gaacatgatc gccatccagg caatgctcgg tccgcggaaa
     5101 taccactgcg aggatggaat gtcgacgcca aaaggctcca gaatcagctg gatcaagccg
     5161 cgcgggtcgc ccaaccagtt gattggggta aagtcgacgc cgatccagcc cagggctgac
     5221 tgcaggaaat aattgatata tccgttgcgc agataaaacc agacgaagat cagggtgatc
     5281 acaactgagg aggtgacgct gggggcatag aaaatggtgc gaaaaaattg tttcgccttc
     5341 atcggagcgt tcagcagaac ggccagtaaa atcgccagac cggtctggat gggtacaacg
     5401 atgaacgagt accacagcac gttatacagg gcctggagga aatcccgcct ggttggatcg
     5461 gtaaagcctt caaaggcagc ggcgtaattc tccaggcctg cccaggtagg gcgagtaaaa
     5521 atatcgtagt tggtgaatga aatatagaag gcaaaaagaa cgacgccgat ggtgaaggtc
     5581 agggtgacta acaagtatgg cgctaaaaag gcgtaggcgg tgagcagctc tgtgcgctgg
     5641 cgtacattcg gccagactct gcgcacctct cgggcggcaa agaaagcgac aacgaccccc
     5701 agcaggccta cagccaggat ataggggatg gcggtggcgc gcacctcggc caggtaagtc
     5761 tgaagagaag ccatgacgcg cggaccgccc attgtgggag cattgaggag agcaatgatc
     5821 agaatagcgg ctacgatacc gataaatacc aacacggata cgagcgtccc ggaagttcgg
     5881 acctgcccgg gagattggcc aggactcctg agattacttt ccatagcgca cctcgatcga
     5941 tctctttcgg gctgcaggtg ccctgctggg gcctgtttac cctgggttcc ccaacaatct
     6001 gccggggaac ccaggagcta tccagtttca cggcggcggt aaacaccgca cccggcggga
     6061 ggaaaccggg ttagccgcca tccagagcgg tttgggcttc ggcttgagcc tgtgcaaaag
     6121 cctcgtcaac ggtctgatcg cccaggaaga tgcgatccag tgcctgggag acagcgtcat
     6181 tcaccttgcc tgtgttcggt ccccagtagg caacgatcgc gtcgggcagc aaaccaccct
     6241 gggctatcgc catatcattg gggtcaacga tcaggtcggc ctgttcgggg tgggtactgt
     6301 aggcaaaacc tgtcttgact atctcagcct ggttttccgg cccggtggca tagaacaaga
     6361 aggctgcggc agcattcggg aagggagtgg cggcattgat cccgatgccg ttggtgaaga
     6421 tgatatcggc cctggttacc ggaccagatg ggatctcaac tgccttccat tcaacttccg
     6481 gataagtggt cttcatgaag ttgaccatcc agccaccttc ggtggtcatg gcaaccgctt
     6541 tcttgccaat cgcttcaccg caccagccgg cgctgacatc ggcggcgcgg accaggctgc
     6601 cctcctggaa catatcattg acgaaggttg ccatcgtctt tacttcgggg gtatccaggg
     6661 tggcagttgt gaaatcaggc gaggcaaacg atccgccgtt ggaggtgaca aaaggtgcga
     6721 atcgcgccca gtctgcgccc atgcaaaagc cgccaaaggt tcccttatcg gcaattgttt
     6781 tggccgcagc gcgcatatcc tcccaggtcc aatcggcggt gggttcggga ataccagcat
     6841 cggcgaacgc ttcgggcagg tagatcagcc ccagcgtacc ccagtctttg ggcagggcgt
     6901 aggtcttccc atccagggtg aaaatcgtca gcagcgctgg aatgaaatca tcgcgagtga
     6961 taccgacctg agccatcagg tcatccagag gcatgatttg ctcggagggg gcaaaggcgg
     7021 tcatcagctg gtcgtcgaca tagaacacat ccggagctgt gccgcctgcc atctgggctt
     7081 tcagcttggt ctgaaagtct gaaggaacgg ggatatagtt caccgttacg tcggggcata
     7141 cgcctttgaa gcgctcaatt gtgtctcgat aaacctgctg ttccgtttca tcaccccaac
     7201 ccgagaagga gatggttgcg cctgcctcca ctggcagagg gcaggttcca gatgccgcag
     7261 ggggcgcggt ggcatcagcc gggggtgcct gagtttcagc cgctggagcc gtcgttgctg
     7321 ccggcgtgcc gcaggctgcg atcagcatgg aaagggcgat cagtacagta aataaagtca
     7381 gggactttct catgggtctt ctcctgagta acatcaaaca ggtcttgtat tccgatatgt
     7441 tatacttaaa caatgcaggg gcaatagtcc ctgtctggga gtcgcctggt atctcttgcc
     7501 ggagaaggcg actccttctg attcaaaacc aaaagggagg ttcatcagga tctgatttgc
     7561 gacacactcc tttctaacag gtggttgtca ggcttcaatt cccgtcgttg attcgcggat
     7621 catcagttgg ggagccacca gctcataaaa tggatccggg aaaacaccgg tttcaaggta
     7681 agcgatcaac ctttccatta aattctgacc gacctgtgag acaggctggt gcaggctggt
     7741 gagcgggggg tttaaataac gagcaatggg cgtatcgtca aaaccggtga tggcgatatc
     7801 ttttccaggc tggaatcctt gctggcgaat tgcagccata gcgccaattg ccatcgaatc
     7861 gttcatggcg acaatcgccg aggggcgagt ggaatcaggc atttctaaaa gccgcagtgt
     7921 gcactggtag ccgacctcgt agctgccttc accacgctgg acccattctt caggcgcttc
     7981 gatgccggct ttctgcatgg ctgagaaata gcccgacatg cggtgattgc cgacccgcga
     8041 atcggtcggc caggcaaggg ctgcgatttt gcggtgaccc agggacagga gatgctccac
     8101 cgccagggat aaccccagct caccgtcaat gtcgatattc gggaagctca gaggattatc
     8161 cagcctgccg aaggcgacga aggggaaatt tcgctggagc aggtaattga tgcaagggtc
     8221 atcgtagtta acaatggaaa gcacgaaggc gtccacgcga cccgtctcaa ttaaatcgcg
     8281 gtaggtggct aaatattcga ttggatcggc atgataagga aaacagagca ggtaataacc
     8341 gcatgactca gcagttcgaa acatgctctg taaaaattcg tccaggatgg ggttggtgtg
     8401 atcgggcggg gaaggctgcc aggaatagcc gatggtttta ctggactgca atttcaggct
     8461 gcgcgcggta tagctgggag tataacccag ttcgtggact gcatcccaaa cgcgctgttg
     8521 tgtgctggca gaaaagtttg cttgatggtt gattaccttc gagacggtct ggtaacttac
     8581 cgcagcgcgt gcagcaactt ctttgatcgt cacccgtttg cgcatagaca ctcctttgga
     8641 gt
//
LOCUS       DATMXK010000124         5305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_106689, whole genome shotgun sequence.
ACCESSION   DATMXK010000124 DATMXK010000000
VERSION     DATMXK010000124.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5305
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106689"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..1567
                     /locus_tag="VN363_02295"
     CDS             137..1567
                     /locus_tag="VN363_02295"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWQ83365.1"
                     /translation="MSIIQNLNTAFKKNPLLGKLVIAGLLIGIALLTGLLAFAGGRSV
                     TLAFSNPGGRAGGPEIGAVDAQGTPLADSANAAAANPNAPSDVPELTPWDGVGRVTML
                     LIGLDYRDWSAGTDYSRSDTMMLLTLDPLTKTAGMLSIPRDMWVAIPGFQHGKINTAY
                     YLGEAYKLPGGGPGLAVKTVEEFLGVPINYYAQVDFGAFVRFIDELGGVKINVPEKIV
                     IDLLGKGSATKKTLQPGVQVLPGEWTLAYARNRYTSGGDFDRGTRQQQVIMAVRDRIL
                     SLELLPGLISRAPALYSELASGVHTNLTLDQIIKLAVLAMDVPDGSIQQGALDEEYVL
                     FGTSPDGLSIVIPLPDKIFTLVDQLFTTSGSLSPQTPGDSLARMQAEAASVALYNGSS
                     RNGLAESTAELLRQQGMNVSQIGSADQPYPRTTLVDHTGSPASLRYLAEALGLGNEPR
                     LILDYNPSAAASVEVYLGEDWAQRAP"
     gene            1709..2320
                     /locus_tag="VN363_02300"
     CDS             1709..2320
                     /locus_tag="VN363_02300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01470.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional precorrin-2
                     dehydrogenase/sirohydrochlorin ferrochelatase"
                     /protein_id="HWQ83366.1"
                     /translation="METQRVIIIGGGSVAARKATSLVDAGACPLVISPDLHPDLEDLL
                     ARDEIRYLARGYQPGDLAGAFLVISATDDPGVNQQVWEAARTAGCLVNIVDDPSRCNF
                     ILPAVVRRGDLSLSISTGGGSPALARRLRERLDAEFGPEYGDLAAILDELRPQLIASC
                     PPDTRLAAALSLVDGPLLEILRRDGYAAAREYAASQINGSQGE"
     gene            2317..3912
                     /gene="cobA"
                     /locus_tag="VN363_02305"
     CDS             2317..3912
                     /gene="cobA"
                     /locus_tag="VN363_02305"
                     /EC_number="2.1.1.107"
                     /inference="COORDINATES: protein motif:HMM:NF004790.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004851 - uroporphyrin-III
                     C-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0019354 - siroheme biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen-III C-methyltransferase"
                     /protein_id="HWQ83367.1"
                     /translation="MSSPRAGIVYLVGAGPGDPGLITLRAVECLRKAEVVVYDRLANQ
                     RLLTYAPTAEWIDAGKLPDHHKLPQEQINLTLITHASQGKVVVRLKGGDPFVFGRGGE
                     EAAALAAAGIPFEIVPGVSSAIAAPAYAGIPITYRDLASSAAFITGHRSDAQADAPGG
                     WRRLAHSADTLVFLMGVQNLPEIVRQLISGGRPPETSVAIIASGTRPEQQTITGTLAD
                     IIDKSSEIRPPAVIVVGEVVNLRQSLRWFDLPQQRPLLGLRVLNPRLQSGRGPDPFSE
                     LLLAAGAQPIDFAASRLAALADPAELDAALQRMADPQTRADWLVFTSANAVHFTLERL
                     AALGLDGRALAGIKLAVIGKATGAALSEWRLIPDFVAQTGTGAGLARELAAATDLRQK
                     RLLIPRSSAALPELPRLLTQSGAVVEAVTAYTLLPVAPDPEALEQVLQRQFDAAAFLS
                     PSALHSLADCLARHGLALPAALKDRWVTCIGETTAAAAKELGITVNSTAETPTLEAVI
                     AALVQARQDLVKMEKELTGDQHA"
     gene            3905..4930
                     /locus_tag="VN363_02310"
     CDS             3905..4930
                     /locus_tag="VN363_02310"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWQ83368.1"
                     /translation="MPENLRALVTGGTGFLGSNLALRLIERRWNVRILERPGANRVLL
                     EGGPFEFVTGDVLQPDTLPPAMQDIDVVFHAAGVVDYWRQGVERMNEVNVAGTRNVMA
                     AALQAGVPRLVHTSSTAAMGIHPNVVVDESFTFNVKPASFVYGYSKFQAEEVVFEYIA
                     RGLPAVIVNPTTVLGPRDIRKVSSGMVVEVVKNCIPPLVPPGGTNIVPICDVAQGHIE
                     AALKGEVGERYILAGENMNHAQLYKTIADVVGCGDTIKVMPRWQVALIAGITDMVQPQ
                     TSGPVPLTGARLRLESQMFYFDGSKARGCFKMPSTPLRTTIERTIEWYTEMGEFDEQP
                     EQEAAPG"
     gene            4927..>5305
                     /locus_tag="VN363_02315"
     CDS             4927..>5305
                     /locus_tag="VN363_02315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83369.1"
                     /translation="MSSKRVRLPRWLIPVQRRFQHFIQIRWLDISLLAKMSSLVITGL
                     LGLMTIFALLGISTASQTTRQAMSDRVMLARMTATSLDTGMRNYHTALTLLASQPAIF
                     DPTLPASEKVLALANLVGFDQGLY"
BASE COUNT         1017 a         1696 c         1606 g          986 t
ORIGIN      
        1 ctccagcccc ggaacctcca accgtgacac ctaccccacc accgcccagc gagactccag
       61 gagagccatc cccgacgacg cccagcgacc agtccgaagc cccgcgtacg gttgagccaa
      121 cgctgcaggt tggaccatga gcattatcca gaatttaaac accgctttta agaagaatcc
      181 actcctcggg aagctggtta tcgccgggtt gttgatcgga atcgccctgc taaccggcct
      241 gctggctttt gctggaggcc gttctgtcac gctcgccttc agtaatcctg gcgggcgcgc
      301 cggtggacct gaaattggcg cagtcgatgc ccaggggact cccctggcag acagcgcaaa
      361 cgcggcagcc gccaacccca atgcacccag cgatgtgccc gagctaaccc cctgggatgg
      421 cgtcgggcgg gtgaccatgc tgctgatcgg cctggattat agggactggt cggcaggcac
      481 ggattattcg cgctcggaca ccatgatgct cctgacactg gacccgctga ccaaaaccgc
      541 cgggatgctt tcgatcccgc gggatatgtg ggtcgcgatc cccggcttcc agcacggcaa
      601 gatcaacacc gcctattacc tgggtgaggc ctacaagctg cctggcggcg ggccgggtct
      661 ggcagtcaaa acggtggaag aattcctggg ggtaccgatc aactactatg cgcaagtcga
      721 ttttggggca ttcgtccgct tcatcgacga gctgggcggg gttaagatca atgttcccga
      781 aaagatcgtc atcgacctgc tggggaaagg ctcggcgacc aagaagaccc tgcagccggg
      841 tgtccaggtt ctgccgggtg aatggacact ggcctatgcc cgcaaccgct acacctcagg
      901 cggcgatttc gaccgcggca cccgccagca gcaggtgatc atggcggtcc gcgatcggat
      961 actcagcctg gagctgcttc ccggtctgat ctctcgggcc ccagccctct acagcgagct
     1021 ggcctccggc gtccatacca acctgaccct cgatcagatt atcaagctgg cagtcctggc
     1081 aatggatgta ccggatggaa gcatccagca gggtgcactg gatgaagagt atgtcctgtt
     1141 tgggacctcg ccggatggat tgagcattgt catcccgctg ccggataaga tcttcaccct
     1201 ggtcgaccag ctcttcacca cctccggcag cctgagccca cagacccctg gcgacagcct
     1261 ggcacgcatg caggccgaag ctgccagtgt ggcgctgtac aacggcagca gccgcaacgg
     1321 gctggcagaa agcaccgccg agcttctccg ccagcaggga atgaacgtca gtcaaatcgg
     1381 cagcgccgac cagccttacc cgcgcacaac cctggtggat cacaccggca gtccggcgag
     1441 tctgcgctac ctggcagagg ccctggggct gggcaatgag ccgcgcctga tcctggatta
     1501 caacccatcg gccgcggcca gtgtggaagt ttacctgggt gaagactggg cgcagcgcgc
     1561 tccctgaccc cgaaaatctc tttttccact caattcagat aaattgggga tttattcccg
     1621 cgccgccagg tgacatatgt cattgacgct tctgcgccgg atatgctaaa actattctgt
     1681 gaaaacatat ccgctgtgct tgatagggtt ggaaacacaa cgcgtgatca tcatcggcgg
     1741 cgggagcgtg gcagcccgta aagccaccag cctggtggat gccggcgcct gtcccctggt
     1801 gatcagcccc gatctccatc ctgatctcga agatttgctc gccagggatg aaatccgtta
     1861 tctggcccgc ggctaccagc cgggagacct ggctggcgcc tttctggtca tttctgccac
     1921 ggatgacccc ggtgtgaatc agcaggtctg ggaggcagcc cggacggcag gctgcctggt
     1981 caatatcgtc gatgatcctt cccgctgtaa ttttatttta cccgccgttg ttcgccgcgg
     2041 cgacctgagc ctgtccatct ccacgggtgg gggcagcccc gccctggccc gccggctgcg
     2101 cgagcgcctg gatgccgagt ttggccccga atacggcgat ctggcagcca tcctggacga
     2161 gctgcgtccg cagctgatcg ccagctgccc gccagacacc cgcctggctg cagccttgag
     2221 cctggtggat ggcccgctgc tggagatcct gcgccgggat ggctatgctg ccgcacgcga
     2281 gtacgcagcc agccagatca atgggagcca gggcgaatga gcagtccccg ggcaggaatc
     2341 gtctatctgg taggcgccgg acctggcgac cccggcctga tcacgctgcg ggctgtggaa
     2401 tgcctgcgca aagcggaggt ggtcgtttac gaccgcctcg caaaccagcg cctcctgact
     2461 tatgccccga ccgccgagtg gatcgacgct ggtaagctgc ccgatcacca caagctgccc
     2521 caggaacaga tcaaccttac cctgatcacg cacgcctccc agggcaaagt cgtggtgcgc
     2581 ctgaaaggcg gcgatccgtt cgtatttggc cgcggcggag aagaagccgc cgccctggct
     2641 gcagccggca tccccttcga gattgtgccc ggggtgagca gcgccattgc tgcgccggcc
     2701 tatgccggca tccccatcac ctatcgcgat ctggccagct cagccgcgtt tatcacaggt
     2761 caccgctcgg atgcgcaggc ggatgcgcct ggcggctggc ggcggctggc tcacagcgcc
     2821 gataccctgg tcttcctgat gggagtccaa aatttacccg agatcgtgcg ccagctcatc
     2881 agcggtggac gcccacccga gacctccgtg gcgatcatcg cctcgggaac tcgccctgag
     2941 cagcagacca tcaccggcac gctggccgat attatcgata aatccagtga aatccggcct
     3001 ccggcggtga tcgtggttgg cgaggtggta aacctgcgcc agagcctgcg ctggttcgac
     3061 ctgccccaac agcgccctct gttgggcctg cgcgtgctta acccccgcct gcagagcggg
     3121 cgtgggccag acccgttcag cgagctgctg ctggcagccg gcgctcagcc catcgatttc
     3181 gcggcttccc gcctggcagc gctcgcagac cccgccgagc tggatgctgc cctgcagcgc
     3241 atggcggacc cgcaaacgcg agcagactgg ctggtgttta ccagcgccaa cgccgtccac
     3301 tttaccctgg agcggttggc agccctcggg ctggatggcc gtgccctggc cggaataaag
     3361 ctggcggtga tcgggaaagc gaccggcgca gcgctttccg aatggcggtt gatcccggat
     3421 ttcgtcgccc agacaggaac aggagccgga cttgccagag agctggcagc tgcgaccgac
     3481 ctgcgccaga agaggctgct gattccgcga tcctcggcgg cgctgcccga gctgccccgg
     3541 ttattaaccc agtccggcgc tgtggtggag gccgtcacag cctacaccct cctgccggtg
     3601 gcgccagacc cggaggccct tgaacaggtc ctccaaaggc aattcgatgc ggctgcattc
     3661 ctcagcccct cagcgctgca cagccttgcc gattgcctgg cccggcacgg ccttgcgctg
     3721 ccagcagccc tgaaagaccg ttgggttaca tgcattggag agacgaccgc ggctgcggca
     3781 aaagaactgg gtatcaccgt caattccaca gccgaaactc ccaccctgga agcggtgatc
     3841 gctgccctcg tccaggcgag acaggatctg gttaaaatgg aaaaagagct cactggagat
     3901 caacatgcct gagaacttgc gtgccctggt caccggaggc acgggctttc tgggctcgaa
     3961 tctggcgttg cgcctgattg agcggcgctg gaatgtgcgc atcctggagc gccccggggc
     4021 aaaccgggtg ctgctggaag gcgggccatt tgaattcgtc accggggatg tgctgcagcc
     4081 cgataccctg ccgccggcca tgcaggatat cgacgtcgtg ttccatgctg ccggggtggt
     4141 cgattactgg cgccagggcg tggagcgcat gaatgaggtc aacgtggcgg gcacccggaa
     4201 tgtaatggca gctgccctgc aggctggtgt accgcgcctg gtgcatacca gctccaccgc
     4261 ggccatgggc atccatccta acgtggtggt agacgaatcc ttcactttta atgtcaaacc
     4321 ggccagcttt gtctatggat actccaaatt tcaggccgaa gaagtggtct ttgaatacat
     4381 cgccagaggg ctgccggcgg ttatcgtcaa ccccacgacc gtattaggac cgcgggatat
     4441 ccgcaaagtc tccagcggga tggtcgtgga agtggttaaa aactgcattc ctccgctcgt
     4501 gccacccggg gggacgaata tcgtgccgat ttgtgatgta gcccagggcc atatcgaagc
     4561 tgccctcaaa ggcgaagttg gcgagcgtta tatcctggcg ggggaaaaca tgaaccacgc
     4621 ccagttgtat aaaaccatcg ccgatgtcgt cgggtgcggc gacacgatca aagtcatgcc
     4681 ccgctggcag gtggctttga tcgccgggat caccgatatg gtccagccgc agaccagcgg
     4741 accggtgccg ctcaccggcg cccgcctgcg ccttgaatcg cagatgtttt actttgatgg
     4801 ctccaaggcg cggggctgct tcaagatgcc cagcacgccg ctgcgcacaa cgatcgagcg
     4861 cacgattgaa tggtacaccg agatgggcga attcgatgaa cagcccgagc aggaggcagc
     4921 cccaggatga gcagcaaaag ggtccgactc ccgcgctggc tgatccccgt ccagcgccgc
     4981 ttccaacact tcatccagat ccgctggctg gatatcagcc tgctggcgaa aatgagctcg
     5041 ctggtcatca ccggcttgtt gggcttgatg accatatttg ctttattagg gatcagcacc
     5101 gccagccaga caacccgcca ggcaatgagc gaccgggtga tgctggcgcg gatgaccgcg
     5161 acctcgctgg ataccggtat gcgcaattat cataccgctt taaccctgct cgccagccag
     5221 cctgccatat tcgatccaac tttacctgcc agcgagaagg tcctggctct ggcgaacctg
     5281 gtaggctttg accagggctt atatc
//
LOCUS       DATMXK010000125         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_60089, whole genome shotgun sequence.
ACCESSION   DATMXK010000125 DATMXK010000000
VERSION     DATMXK010000125.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60089"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..379)
                     /locus_tag="VN363_02320"
     CDS             complement(<1..379)
                     /locus_tag="VN363_02320"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWQ83370.1"
                     /translation="MTPLALQRMHNQFLAASHPGSLGELVCALGAVQGQDHQGTLWSL
                     GLRMGGGLDQAQVEAGYAQPAGKATIVRSWVLRGTLHFVASGDLGWMVALLAPRLLNG
                     EARRYRELELDEATRRCSDKLLVK"
     gene            589..777
                     /locus_tag="VN363_02325"
     CDS             589..777
                     /locus_tag="VN363_02325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83371.1"
                     /translation="MATYPQCSYAHLAEDARFCSNCGARIEIASPPAAESSQNLVQVK
                     MQVEQIAGGFTFMFQRTG"
     gene            820..1896
                     /locus_tag="VN363_02330"
     CDS             820..1896
                     /locus_tag="VN363_02330"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ83372.1"
                     /translation="MKQLIRFCTTPDAVRIAYSTTGDGPPLVKAANYLTHLEHDWNSP
                     VWRHWLLGLSATHRLIRYDARGSGLSDWDIASYSMDDWVRDLEAVVDTLQLEQFPLLG
                     ISQGAAVCIAYAVKHPERVSRLVLYGGYARGRFHRNLDAQGQLEAEALINLIRVGWGQ
                     ENPAFRQLFSTMLMPDADLEQMASLNQLAQISATPENASRMERAFYQIDVREQAAKVS
                     MPTLILHPRRDGSIPMEESRLLAAMIPDSRFVVLESRNHILLEQEPAWSRFLAEVRTF
                     LGVGAEPVEQPPLHPAVFPELTPRERVILEMIAQGLDNTQIAAQLVVSQRTVRNHISH
                     IFRKLRIRNRAQAIVLARKSGLGH"
     gene            2027..>2713
                     /locus_tag="VN363_02335"
     CDS             2027..>2713
                     /locus_tag="VN363_02335"
                     /inference="COORDINATES: protein motif:HMM:NF017505.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008430 - selenium binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-binding protein SBP56-related protein"
                     /protein_id="HWQ83373.1"
                     /translation="MNSHATDSQASHASHAMHSHGDDPQAEDMLAEDPHAHCGPGYAS
                     PRDALQAGREKLLYVNALYTGTGIDQPDYLATVDADPDSPTYSQVIYRTPMPNIGDEL
                     HHFGWNACSSCHTDSSKSRRYLILPGFRSSRIHILDTSEPRAPRLHKVIEPEEVLQKT
                     SLSAPHTVHCLADGTVMISMLGDQDGNAPGGFLLLDENFDVLGRWDSQQAGMDFNYDF
                     WYQPRHNVMVS"
BASE COUNT          544 a          841 c          771 g          557 t
ORIGIN      
        1 tctttaccag cagcttatcg ctgcaccggc gggtcgcctc atccagctcc agctcacggt
       61 agcggcgcgc ctccccattc agcaggcggg gagccagcag cgccaccatc cagcccaggt
      121 cgccggaggc aacgaagtgc agcgtgccgc gcagcaccca cgagcgcaca atggttgctt
      181 tcccagccgg ctgcgcatag ccggcttcga cctgggcctg gtccaatccg ccgcccatgc
      241 gcaagcccag cgaccacagc gtgccctggt gatcctgccc ctgtaccgct cccagggcac
      301 agaccagctc gcccaggctg ccgggatgag aggcagcgag gaactggttg tgcatgcgtt
      361 ggagggcgag gggtgtcatt atttccacct gtaatactga aataccttct gcaggctacc
      421 tggcttactt gctatttggg cagacctcga tccagaacac cgtgatcata ggcagaaaaa
      481 tgcaaattgt caatggacct cggaaaatat tggacacgaa cagtcagcaa tgcgattttc
      541 tgatatgata ttaaggaccc ttctcctttt tacttccggg aggcgcgtat ggcaacctat
      601 ccgcagtgca gttatgccca tctggccgaa gatgcccgct tctgctccaa ttgtggggcg
      661 cggattgaaa tcgcctcgcc gccggctgca gaaagctcgc agaacctggt ccaggtcaag
      721 atgcaggtgg agcagatcgc cggcggcttc accttcatgt tccagcgtac cggttagctg
      781 gttgcgtacc ctgtacccga gccagcatcc agaccagaca tgaaacagct gattcgattc
      841 tgtaccactc cagatgccgt ccggattgcc tattccacca ccggcgatgg cccacccctc
      901 gtaaaagctg ccaattacct gactcacctt gagcacgatt ggaacagccc ggtctggcgg
      961 cactggctgc tgggtttatc cgcaacccac cgcctgatcc gctatgatgc ccgcggctca
     1021 gggctctcgg attgggatat tgcttcctat tcgatggatg attgggtgcg cgatctggaa
     1081 gcggtggtgg ataccctgca gctagagcag ttcccgctgt tgggtatctc gcagggcgcg
     1141 gcagtctgca tcgcctatgc cgtcaagcat cccgagcgtg tctcacggtt ggtgttgtat
     1201 ggcggatatg cgcgcgggcg ctttcaccgc aacctggacg cccagggaca gctggaggct
     1261 gaagccctca tcaatctgat ccgggtgggc tgggggcagg aaaacccggc tttccgccag
     1321 ctgttcagca ccatgctgat gccagacgcc gacctggagc aaatggcctc attaaaccag
     1381 cttgctcaaa tctcagccac ccctgaaaac gcctccagaa tggaacgcgc tttttatcag
     1441 attgatgttc gggagcaggc agcgaaagtc agcatgccaa ccttgatttt acatcctcgt
     1501 cgggatggaa gtattccgat ggaggagagc cggctgctgg cggcgatgat cccggactcc
     1561 cgcttcgtgg tattggagag ccgcaatcac atcctgctgg agcaggagcc tgcctggagc
     1621 cgttttctgg cagaggtgcg caccttcctg ggcgttggcg ccgaacctgt tgagcagccg
     1681 cccctccatc cggcggtctt cccggagctc acgccgcgtg agcgggtgat cctggagatg
     1741 atcgcccagg gattggataa cacccagatc gcggcgcagc tggtggtcag ccagcgcacg
     1801 gtgcgcaacc atatctcgca tatcttccgc aagctcagga tccggaatcg cgcccaggcg
     1861 atcgtcctcg cccggaagtc cggattggga cactagtccc ataaatttca cgattttcgg
     1921 cccacaatcc catcgaaaat aagcctctgg tctcaagaca gctccgggtg atcaggatat
     1981 aaccgggtta tatttacccg ttgtctgaaa ctactggagg tttattatga attcccacgc
     2041 aaccgattcc caagcatccc atgcatccca cgccatgcac tcccacgggg atgacccgca
     2101 ggcggaggat atgcttgcgg aggatccgca tgcgcactgc ggccccggct atgcctcgcc
     2161 gcgtgatgcg ctgcaagccg ggcgagaaaa actgctgtac gtcaacgccc tctacacggg
     2221 cacaggtatc gaccagccgg attacctggc cacggtggat gccgatcccg attcgcccac
     2281 gtattcgcag gtgatctacc gtacgcccat gcccaatatc ggcgatgagc tgcaccactt
     2341 tggctggaat gcgtgcagct cctgccatac ggacagcagc aaatcccggc gctacctgat
     2401 cctccccggt ttccgttcca gccggatcca tatcctggat acctccgagc cgcgcgcgcc
     2461 ccgcctgcac aaggtgatcg agccggaaga ggtgctgcag aaaaccagcc tgtctgcgcc
     2521 gcataccgtg cactgcctgg cggatggcac ggtgatgatc tccatgctcg gcgatcagga
     2581 tggcaatgct cccgggggtt ttctcctgct ggacgagaat ttcgatgtcc tggggcgctg
     2641 ggattcccag caggcgggga tggattttaa ttatgacttc tggtaccagc cgcgccacaa
     2701 tgtaatggtg tcc
//
LOCUS       DATMXK010000126         3792 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_2719, whole genome shotgun sequence.
ACCESSION   DATMXK010000126 DATMXK010000000
VERSION     DATMXK010000126.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3792)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3792)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3792
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2719"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            114..344
                     /locus_tag="VN363_02340"
     CDS             114..344
                     /locus_tag="VN363_02340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83374.1"
                     /translation="MFEETGDLQPTSRTWANAQAFASMNNGYLAQYGVSAVVTGITVD
                     AGEDPVLVQVSANLERLFPSVVPGVSSLSKVP"
     gene            374..943
                     /locus_tag="VN363_02345"
     CDS             374..943
                     /locus_tag="VN363_02345"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWQ83375.1"
                     /translation="MPKARDYQMNEAELQAVEIAIRRDKRPEVRQRCTAMRLLHLGHK
                     PAQVAEMQAVSIPTIYAWINRWRKGGIEALATQPRSGRPPKADPAYSQLLGEVLEKEP
                     AELGYGFTMWTIDRLRAHLAKETGIELSESRFRALLKREGFRYRRPKLDLNHLQDPEA
                     KAQADELLEGLKKRSLETISGSSLWTKPR"
     gene            925..1434
                     /locus_tag="VN363_02350"
     CDS             925..1434
                     /locus_tag="VN363_02350"
                     /inference="COORDINATES: protein motif:HMM:NF033545.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS630 family transposase"
                     /protein_id="HWQ83376.1"
                     /translation="MDETTLTLDPPLRACWMKIGQQKRIPATRPGMKQKRHVFGGYDW
                     VNDTITWTVAETKNSSQFIQFLEHLLVTQYPTGRVVLVMDNAAYHKSAPSMAALSLFE
                     HRVLVIWLPTYCSELNPIERYWRHLKDLACANKLQDNIDEVVRAAENTMTNQNTLEYA
                     SRYHVSRNL"
     gene            complement(1724..2539)
                     /locus_tag="VN363_02355"
     CDS             complement(1724..2539)
                     /locus_tag="VN363_02355"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF014228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sir2 family NAD-dependent protein deacetylase"
                     /protein_id="HWQ83377.1"
                     /translation="MTEVNIQTQLERATRLLAGSRFAVAFTGAGISTPSGIPDFRSPD
                     TGLWARLEASLEGEPQVGTVHSFARDPQTFYERMKPLFQRIVTAGPNPAHHALADMEK
                     LGIIQAVITQNADMLHQRAGSQCVIEVHGTLAEATCIRCYKVRPFRPLLERFFADGQV
                     PTCKDCGGVIKPNVILTGEELPARAFLAARQALRQCDVLLVTGTSLAGGPATALVETA
                     HAQGTKLIIVNLTPTLMDTVVEIAIQADVVTALPAIAIRLAKDREATVSAILR"
     gene            complement(2532..3245)
                     /locus_tag="VN363_02360"
     CDS             complement(2532..3245)
                     /locus_tag="VN363_02360"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ83378.1"
                     /translation="MKVRYLGHSCVEIIGQHHILIDPDYTRGPEPEVEYTLVTHAHKD
                     HIGRLSEVTSGKVVASPDVCEIAVRMGVDRERMLPVSPGKQIANIQVLPGYSLVDDPI
                     YKLFYVLFRWRRPDPGGTPLSFLIKDAATLLHIGDAHDAPLPVRPDILCLPWRKSPLW
                     TKRYQATLIRMAEKFAAPYVLPIHYDLPHSRADPRSLNGRLAAEILDGNGWYSFRNRQ
                     RVQDGDWVSAISRREIKGD"
     gene            complement(3242..3688)
                     /locus_tag="VN363_02365"
     CDS             complement(3242..3688)
                     /locus_tag="VN363_02365"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWQ83379.1"
                     /translation="MMEKIREHGSCFVCGKANPNRIGVEWYFDDGNHIEGSFIFTIHH
                     QGPPGFVHGGASAAVLDEAMGASVWRAGFRAAAVNLNTTFIKPVPLEVRVQVIGEFIE
                     KKDKHIFTQGEIRLPDGTVAVKSKGVYVEAPHLFNELTSHFEARQS"
BASE COUNT          911 a         1002 c         1015 g          864 t
ORIGIN      
        1 tctgatgctg gatcctcgga ccggacaatc gaaatcgccc ttcaggcagc ctgccgggta
       61 gtgcctgtgg ggatgccgtc gccctggccg ctgctgccga gatcagccag agggtgttcg
      121 aggaaaccgg ggatctacaa cccaccagcc gcacctgggc caatgcccag gcttttgcca
      181 gcatgaataa tggttactta gcgcagtatg gtgtgtcggc cgtagtgacg ggaattacag
      241 tagatgcagg ggaggatccg gtgctggtgc aggtttcagc aaatttggag aggttgttcc
      301 cgagtgtagt gccgggggta agtagtctgt cgaaagtacc ttaacaaacc gtcatttatg
      361 ataaactcgg ggcatgccaa aagcccgaga ttatcaaatg aatgaagcag agttgcaggc
      421 ggttgagatc gccatccgcc gcgacaaacg tccggaagta cgtcaaaggt gcacagctat
      481 gcgcctcctt catctgggac acaaaccggc tcaagtcgca gaaatgcaag cagtcagcat
      541 cccaacgatc tatgcttgga tcaaccgctg gcgcaaggga ggtattgaag ccctggccac
      601 ccaaccccgc agtggaagac cgcccaaagc tgatccagct tacagccagc ttttgggaga
      661 ggtattggaa aaagaacccg cagagctggg gtatggtttc accatgtgga cgatcgatcg
      721 cttgcgtgct catctggcga aagaaaccgg gattgaactg agcgaaagcc ggtttcgggc
      781 tttgctcaag cgggaagggt ttcgatatcg acgccctaag cttgacctga accatctgca
      841 ggacccagaa gccaaggcac aagcggacga gttgctggaa ggcttgaaaa aaaggtcctt
      901 ggagacgatt tcaggctcat ctttatggac gaaaccacgt tgacgctcga cccaccactg
      961 agagcttgtt ggatgaagat cgggcagcag aagcggatcc cagcgacccg acctgggatg
     1021 aaacagaaac ggcatgtgtt tggtggctac gattgggtca acgacaccat cacctggacc
     1081 gttgccgaaa ccaagaacag ctcgcagttt atccagtttc tggaacacct gctggtcaca
     1141 caatacccta cggggcgagt ggttctggtg atggataacg ccgcttacca caaaagtgct
     1201 ccttctatgg ctgcattgag cttgtttgag catcgcgtct tggtcatttg gttaccaacg
     1261 tactgctccg aactgaatcc gattgaacgt tattggcgcc atctcaaaga cctggcatgt
     1321 gccaacaagc tgcaagacaa cattgacgaa gtggtccggg ctgctgagaa caccatgacg
     1381 aatcagaata cgcttgagta tgcatctcga taccatgttt ctagaaactt atgacggttt
     1441 acttagttgt gactgaggcg ggttatgctg aaattcgagt gtttacccgt tgaagtttat
     1501 actttagatt ctacaggaag atcgaaacaa ctgacttcgg gctatgaagc cagccccaat
     1561 ttggcttgct ttcgcattca caaccgaaac gaaacacctc tgcttcacga tccgaatgga
     1621 ctcaccctaa agctggtaat tcgatagtgc tatgcactcc ctccccagtt atggagtaag
     1681 ttcgcgccaa aatcacgtta tgcattttca tcaattgctc aaactaccgt aggatggcgg
     1741 atactgttgc ttctcgatct ttggcaagtc tgatggcgat cgcgggcagg gccgttacga
     1801 catctgcctg gatcgcgatt tcgacaacgg tatccatcag agtgggggtc agattgacga
     1861 tgatcaattt cgtaccctgt gcgtgagcgg tttccactag tgccgtcgcc ggcccaccag
     1921 cgagcgaagt cccggtcacc agtaatacgt cgcattggcg gagggcctga cgcgccgcca
     1981 gaaatgccct ggccggcaac tcctcaccgg tgaggatcac gttgggcttg atcacgcctc
     2041 cgcagtcttt gcaggttggg acctgcccat cggcgaagaa gcgctccagc aagggacgaa
     2101 agggacggac tttgtagcat cggatacagg tagcctcagc cagggtgcca tgcacctcga
     2161 tcacgcattg cgaccccgct cgctggtgga gcatgtccgc attttgggta atgacggcct
     2221 ggatgatacc cagtttctcc atatcggcca gcgcatggtg agcgggattc ggccctgcgg
     2281 ttacgatcct ttggaacaaa ggtttcatcc gctcgtagaa cgtctgcgga tcacgggcaa
     2341 atgaatgcac cgtcccgacc tgcggctccc cctcaagcga ggcttccagg cgcgcccaca
     2401 gtcctgtatc tgggctgcga aaatcgggga tgccagaagg cgtagagata cctgcaccgg
     2461 taaaagcaac cgcgaaacgg ctgccggcca aaagccgggt tgctctttct agctgggttt
     2521 gaatattaac ctcagtcacc ctttatttct ctccttgaaa tggctgaaac ccaatctcca
     2581 tcttgcaccc tctgccggtt gcgaaagctg taccagccgt ttccgtccag gatttcagct
     2641 gccagtcgcc cattcagcga ccgggggtcg gctcttgagt ggggcaagtc gtaatggatc
     2701 ggcagcacgt atggagcagc aaatttctcg gccatgcgga tcagggtcgc ctggtagcgc
     2761 ttagtccaaa ggggtgactt tcgccagggc aggcaaagaa tatccggcct gacaggaaga
     2821 ggcgcatcat gggcatcacc aatgtgcagt aaagtcgctg catctttaat tagaaacgag
     2881 agcggtgttc cgcccggatc aggccttcgc caacgaaata acacatagaa aagcttgtat
     2941 atggggtcat caacgaggct atatccaggt agaacttgaa tgttggcgat ttgcttccca
     3001 gggctgactg gtagcatacg ttcccgatcg actcccatcc tgacagcaat ctcgcatacg
     3061 tcgggggatg ccacaacctt accactcgtg acctcggaca acctgccgat gtgatcctta
     3121 tgggcatgcg taaccagcgt gtactccacc tccggttctg gacctcgggt gtaatccgga
     3181 tcgatcaaga tgtggtgctg cccgatgatc tcaacacaag aatgccccag gtagcgtact
     3241 ttcatgattg ccttgcttcg aagtggctgg taagttcgtt gaacagatga ggcgcttcca
     3301 catagacacc cttactttta acagccaccg tgccatccgg aaggcgaatt tcaccctgag
     3361 taaagatgtg cttgtccttc ttttcgatga attccccgat tacctggact ctcacttcca
     3421 gtgggaccgg ttttataaag gtcgtattca ggttgaccgc cgcggcgcga aacccggcgc
     3481 gccagacaga tgcgcccatg gcctcgtcta atactgctgc tgatgcacct ccgtggacaa
     3541 aaccaggagg gccttggtga tgaatggtga agataaaact accttcaatg tggtttccat
     3601 catcaaaata ccattctacg ccaatacgat tggggttagc tttaccacac acaaaacacg
     3661 aaccatgttc acggattttt tccatcatga aaagtccagt ttgcgcctaa ttatgctgat
     3721 gaggttctct ttgtatccag ctcttgtgat cttgaatatc acgatctaag aaaatccgac
     3781 ggagaatcac at
//
LOCUS       DATMXK010000127         2016 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_146221, whole genome shotgun sequence.
ACCESSION   DATMXK010000127 DATMXK010000000
VERSION     DATMXK010000127.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2016)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2016)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2016
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146221"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(130..1293)
                     /locus_tag="VN363_02370"
     CDS             complement(130..1293)
                     /locus_tag="VN363_02370"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWQ83380.1"
                     /translation="MKLNPSLKIINWLRGIGKNADFSEQPDPTFLRADGLPNIVISKN
                     DPLVEYLEKTPVIADVDRLTIDSPTLGTLRNSGVKLITPLVSSGELLGILILSSKRSE
                     QEYSNDDRQLINMLAAQVAPTVRVAQLVQQRQAVLLEKERLDQEMRLARLIQQTLLPK
                     TPPELAGWQVALHYQPARAVGGDFYDFFPLPDGKIVIVIGDVTDKGVPAALVMASTRA
                     ILRGSARRMLSPGEALKRSNELLLPEIPSRMFVTCLYAILDVNTGVITYANAGHNLPV
                     GCHAGQIRELRATGMPLGLLENMEYDEFTATIQPGESLLFYSDGLVEAHNPGGEMFGF
                     GRLHSKMASFDHVGETLVDYLLADLESFTGEGWEQEDDVTLLTLHFQAPGGEQ"
     gene            complement(1294..>2016)
                     /locus_tag="VN363_02375"
     CDS             complement(1294..>2016)
                     /locus_tag="VN363_02375"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83381.1"
                     /translation="AAVLNDMQNPWQYIIAWRNSYLYLSLGILVFLVMMVVRQRFQNS
                     PIIRQQTRIVLLGGLLAFVPIALWMGAPVLGLNIRWNPDLFLPFLLIFPLSIAVAIQR
                     YRLWDIDGLINRAMVYGALSIILGILFSGSVLILQRLFTVITGSQSNLAAIISTLLIA
                     ALFNPLRIRMQEYIDRRFYRHKYDAEQTLIAFSLAIRDEVDLEKLSERLIGVIDETLE
                     PDRAAFITIDRSETSRPAIESN"
BASE COUNT          525 a          503 c          500 g          488 t
ORIGIN      
        1 cgataatatg agagaccagc tcgaccgcca gtttgtccgc attgcgttgc cggggaagat
       61 taatttcaga gaggaccttc cactccccat ggctgttcgc tgtttctgga agatcaggta
      121 tagaggatgt cattgttctc ctccaggcgc ctgaaaatga agagtcaata acgttacatc
      181 gtcttcctgt tcccagccct ctcctgtaaa ggattcgaga tccgctaaaa gataatcaac
      241 cagagtctca ccaacatggt cgaaggatgc catttttgaa tgcagccggc caaaaccgaa
      301 catttccccc ccaggattat gggcttctac cagaccatca ctataaaaca atagactctc
      361 accgggttgg atggtggctg tgaattcatc atattccata ttttccagga gtcccagagg
      421 cattcccgta gctctcagct cgcgaatttg cccagcgtgg cagccaaccg gtaaattgtg
      481 tccagcgttg gcataggtta ttacccccgt attgacatct aatatcgcgt aaaggcaagt
      541 aacgaacatt cgtgacggaa tctcaggcag gagcagttca ttcgatcttt tgagcgcttc
      601 gccaggagat aacattcgcc gcgcagagcc gcgcaggatg gctcgtgtgg aggccataac
      661 cagggctgcc ggcacgcctt tgtcggtgac atccccaatc acaatcacaa tctttccatc
      721 cggaagcgga aagaaatcgt aaaaatcccc accgacagct cgcgcaggct gataatgtaa
      781 agctacctgc catccagcca attccggtgg agttttcggg agcagggttt gttggatcag
      841 acgcgccagg cgcatctctt gatccagacg ctctttttcg agcagcactg cctggcgctg
      901 ttgaacaagt tgcgccaccc ggacagtagg agcaacctgg gctgccagca tattgatgag
      961 ctggcgatcg tcatttgaat attcctgctc gctgcgtttg gagcttaaaa ttagaattcc
     1021 gagcagctca ccactgctca ccagaggtgt aattagtttg acgccactat tccgcaaggt
     1081 gcctaaagtg ggcgaatcga ttgtgagcct gtcaacatct gcaattacgg gcgttttttc
     1141 taaatattca accagcggat cgttctttga gatcacgatg ttcggcagac catcagcgcg
     1201 taaaaaggtg ggatcgggtt gttcgctgaa atctgcgttt ttaccgattc ccctcagcca
     1261 gttgatgatt tttagactgg ggttcagttt catctaatta ctctcgatcg ccggtcgaga
     1321 cgtctcactg cggtcgatcg tgataaatgc agccctatcc ggctccaggg tttcatcgat
     1381 cacgcctatc aggcgttcag aaagcttttc taaatcaacc tcatcccgga tcgccaaaga
     1441 aaatgcaatc agggtttgtt cggcgtcata tttgtgcctg tagaaccgcc ggtcgatata
     1501 ctcctgcatt cggatgcgca gcggattaaa cagagcggcg atgagaaggg ttgatatgat
     1561 cgcagcaagg ttcgattggg agccggtgat taccgtaaat aagcgctgta agatcaaaac
     1621 actgccactg aagaggatgc cgaggatgat cgacagtgcg ccgtatacca ttgcccggtt
     1681 gatgagccca tcaatatccc ataaacggta acgttggatg gcgacggcaa tcgataacgg
     1741 aaagatcaat agaaatggca ggaatagatc aggattccag cgaatattca gtccaagtac
     1801 cggtgctccc atccataagg caatcggaac gaaggctaat aatccaccaa ggaggacaat
     1861 ccgggtttgc tggcggatta ttggagaatt ctgaaagcgt tggcgaacga ccatcataac
     1921 caggaagact aaaatcccta atgataagta aagatagctg tttctccagg cgataatgta
     1981 ctgccaggga ttctgcatat cattaagcac tgcagc
//
LOCUS       DATMXK010000128         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_168462, whole genome shotgun sequence.
ACCESSION   DATMXK010000128 DATMXK010000000
VERSION     DATMXK010000128.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168462"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..469
                     /locus_tag="VN363_02380"
     CDS             <1..469
                     /locus_tag="VN363_02380"
                     /inference="COORDINATES: protein motif:HMM:NF024724.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWQ83382.1"
                     /translation="LSVAALVLEDGGSEDEAIAALLHDAVEDQGGQATRAEIERRFGG
                     QIAEWVMALSDSDTIPKPPWRKRKEAYLEHLSAAVPEVLRISLADKLHNARSILEDMR
                     SEGPETLGRFNGGKTGTLWYYRRLVAIFNARLASPRAAELERVVSELQVLVRK"
     gene            485..1180
                     /locus_tag="VN363_02385"
     CDS             485..1180
                     /locus_tag="VN363_02385"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWQ83383.1"
                     /translation="MAKFLISGLINLETTLQVEAFPLTYEPVRYPFFGVASTVSGVGY
                     NITKALQTLGDEVAFLSLIGDDAPGWLVRQALQQDGIPGVDVLDCPSPTAQSVILYEP
                     GGRRQIHVDLKAIQEQVYPLERFQQVLERCNPELLVIGRGAQGALLAVREDSFIGSFP
                     APSLRPVVNTIGAGDAQFACFLHEYLTSPDPYRALQRAQVFAACKIGSAGAAQGFLDA
                     PSLEELCSRHGIS"
     gene            1189..1902
                     /locus_tag="VN363_02390"
     CDS             1189..1902
                     /locus_tag="VN363_02390"
                     /inference="COORDINATES: protein motif:HMM:NF013232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83384.1"
                     /translation="MSANPKSRIVILISADGEWQAVLNHFPAARLEKSPYGDWFQTAI
                     PDLEPGTSPRPVIFFHGGWGKIASAGATQYAIDYWKPAALVNLGTCGGFEGQVEKGEI
                     LLVERTVVYDIIEQMGDFEAHIAHYATRIDLSWLQPPYPQPVRRTLLVSGDRDLLVDE
                     IPELGRRYGAVAGDWESGAIAWVAARQGVRLLILRGVSDVVGAAGSAAYGDLTYFQHS
                     AAVVMDRLLAALPLWLACL"
     gene            2000..3661
                     /locus_tag="VN363_02395"
     CDS             2000..3661
                     /locus_tag="VN363_02395"
                     /inference="COORDINATES: protein motif:HMM:NF012398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde dehydrogenase family protein"
                     /protein_id="HWQ83385.1"
                     /translation="MSIPRVLEFVNPATGERFGEIPAAAPEEIPQAIQELRAAAPAWA
                     NRPLDERIRILRKFLGLLIDARDEITAVINQDTGKSRQDALVEVLIQADYLHTYLKHA
                     PRWLRREAVSSELYFTKRAAIEHRPYGVVLVLAPWNYPLVLALSPLLGALLAGNVVVL
                     KPSEVAPATGAMIAKLLGSIPELAPVTRVLHGDGAVGAALVAARPDYIFLTGSTATGK
                     AVSLAAAEGLIPGSYELGGKDAMLVLEDADLPAAAHWGVWAAHYNAGQSCVAVERAYV
                     VQPVFEQFVKLAVAETQALEQGFSTAKNSHFNLGPITDPRQLDTVEKHLKDALQKGAH
                     LLTGGERRGSYITPAVLVDVDESMLIMQEETFGPVLPILAVANEDEAIARTNTSSFGL
                     GVSIWSRDLKRARRVASRLKSGAAVINDAIAHFAVPLLPFGGVDDSGYGRTHGKEGLL
                     QFTYSFAILEGGPPLAWDIATVARQAGRYDLVAGILRLAFGVSSRQRLEPVVETLAQA
                     RELSHKDWSTVQRRLAPFKPALGAATLALGAFAGAALFAGKRRKH"
     gene            3735..>4865
                     /locus_tag="VN363_02400"
     CDS             3735..>4865
                     /locus_tag="VN363_02400"
                     /EC_number="5.1.99.6"
                     /inference="COORDINATES: protein motif:HMM:NF015791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-hydrate epimerase"
                     /protein_id="HWQ83386.1"
                     /translation="MHARLVSVSEMVAIERAAAAAGHGYARMMEFAGRGLAEEVEEAY
                     DFLRSEGALGLVGSGNNGGDTLVALDWLAQWGWTATACLVRPRPADDPLVTRLLASGG
                     EVFSLEGDSSTTHLYERLASCGIVLDGVLGTGMRLPLKVEVAQALEHVRQALDGLEDP
                     PVVVAVDCPSGVDCDSGEAAPETLPANLTVTMAAYKQGLLKFPAYDYVGELRLVGIGL
                     PQDGDGLEPYRSLQITVPDMDDVRALLPLRPAQAHKGTFGTALIVAGSQNYTGAVLLA
                     GEAAYRIGTGLVTLAVTEMIQPMLAGHIPEATWLVLPEAGGGVSPAAARVILDNLERV
                     TGLLIGPGFGLASPTAEFIARFLAGGQYPVQLPVLPPLVVDAD"
BASE COUNT          873 a         1383 c         1563 g         1046 t
ORIGIN      
        1 gttaagcgta gctgcgctgg ttctggaaga tggcggcagt gaagacgaag cgattgcagc
       61 tttactgcac gatgcggttg aggaccaggg cggacaggct acccgggcag agatcgagcg
      121 ccggtttggc gggcagattg cggagtgggt gatggcgttg agcgattcgg ataccatacc
      181 gaagcctccc tggcgaaagc gcaaggaggc atatctggag cacctgtctg cggcagtccc
      241 cgaagtgctg cgcatctccc tggcggacaa gctgcacaat gcccgcagta tcctggagga
      301 catgcgcagt gaaggcccgg agaccctggg gcgcttcaat ggcggtaaaa ctggcactct
      361 ctggtactac cgcaggctgg ttgcaatctt caatgcgcgc ctggcttccc cacgtgccgc
      421 cgagctggag cgagtggtca gcgagctgca ggtgttggtc cgaaagtaat cctggcagcc
      481 ctgtatggcg aaatttctca tttccggtct gatcaatctg gaaaccacct tgcaggtgga
      541 ggcgttccct ctcacctacg agccagtgcg ctaccctttt tttggcgtcg ccagcacagt
      601 ctcgggggtg ggctacaata tcaccaaagc cctgcaaacg ctgggtgatg aagtcgcatt
      661 tctgtccctg atcggggatg atgctccggg atggcttgtc cgccaggctc tccagcagga
      721 tggtattcct ggtgtggatg tgctggattg cccttcgccg actgcccaat ccgtaatcct
      781 gtatgagcca ggtgggcggc gccagatcca tgtcgacctc aaagcgatcc aggagcaagt
      841 ttatccgctg gagcggtttc agcaggtgct ggagcgctgc aaccccgaac tcctggtgat
      901 cggtcgtggg gcgcaagggg cgctgctcgc tgttcgggaa gatagcttta tcgggtcgtt
      961 cccggcccct tccctgcgcc cggtggtaaa caccatcgga gcaggggatg cccagtttgc
     1021 gtgtttcctg catgaatatc tcacctcacc cgacccatac cgggcgctcc aacgggctca
     1081 ggtctttgct gcctgcaaga ttggtagtgc gggcgcagcc cagggcttcc tggatgcacc
     1141 ctccctggaa gagctgtgca gccggcatgg cataagctga ggcctgaaat gagcgctaac
     1201 ccaaaatccc gtatcgtaat cctgatctca gccgatggtg aatggcaggc ggttttaaat
     1261 cacttcccgg ctgccaggct ggagaaaagt ccctatggag actggtttca aaccgctatc
     1321 cccgatcttg agccaggtac gtccccgcga ccggtgattt tctttcacgg cgggtgggga
     1381 aagatagcct ccgccggcgc gacccaatat gccatcgact actggaagcc cgcagccctg
     1441 gtgaacctgg gtacctgcgg cgggtttgaa gggcaggtcg aaaaaggcga gatcctgctt
     1501 gtggagcgca cggtggtgta cgatatcatc gagcagatgg gtgatttcga ggcccacatt
     1561 gcccattatg ccacccggat cgatctttcc tggctgcagc cgccttaccc ccagcctgtc
     1621 cggcggaccc tgctggtatc cggagaccgc gatctcctgg tagatgaaat tcctgaatta
     1681 ggcaggcgct atggagcggt tgccggggat tgggaatcgg gggcgattgc ctgggtggca
     1741 gcccgccagg gtgtgcgctt gctgatcttg cgcggggtaa gcgatgtggt tggagcagcg
     1801 ggcagtgccg cctatgggga tctgacctat ttccagcatt ccgcggccgt ggttatggat
     1861 cgcctgcttg ccgccctgcc gctctggctg gcgtgcctgt gagggtgctg gaaccactcc
     1921 tcccccattt tcgtttattc gagctatggt aaactggttg gagcgctgat ttattcgaga
     1981 agttgcccag gaggctgaca tgagcatacc ccgggtcctg gaatttgtga atcctgccac
     2041 cggtgagagg ttcggcgaga ttccggctgc ggcgcccgaa gagatccctc aggcgattca
     2101 ggagctgcgc gccgcagccc cagcctgggc gaaccggcct ctggacgagc gaatccgaat
     2161 cttacgaaaa tttttgggcc tgctcattga tgcgcgcgat gagatcaccg ccgtgatcaa
     2221 tcaggacacc ggcaagagcc gccaggatgc gctggtcgaa gtcctgatcc aggccgatta
     2281 tttacatact tacctgaagc acgccccgcg ctggctgcgc cgtgaagcgg tctcaagcga
     2341 gctgtatttc accaagcggg ccgccatcga acaccgccca tatggggttg tgctggtcct
     2401 ggctccctgg aattacccac tggttctggc actttccccg ctcctgggag ccctgttagc
     2461 tggcaatgtc gtggtgttga aaccttcgga ggtagctccg gcaaccgggg cgatgattgc
     2521 aaaacttctt ggcagcattc cagagctggc gccggtgacc cgcgtcctgc acggcgacgg
     2581 agccgtcggt gcagctctcg ttgcagccag accggattat atcttcctga ccggttcgac
     2641 cgcgaccggc aaagcggtca gcctggcagc cgccgaaggc ttgatcccgg gcagttatga
     2701 gctgggcggg aaggatgcga tgctggtgct tgaggacgcc gacctgcctg ccgcagccca
     2761 ttggggtgtg tgggcagccc actacaacgc cgggcagtcg tgtgtggcgg ttgagcgcgc
     2821 ttatgtggtg cagccggttt tcgagcagtt tgtaaagctg gctgttgctg agacccaggc
     2881 cctggagcaa ggcttctcaa ccgccaaaaa tagccatttt aatctgggac cgattaccga
     2941 tccgcgccag ctcgacaccg tggaaaagca tttaaaggat gccttgcaaa aaggtgccca
     3001 cctgcttacc ggtggggagc gccgcggcag ctatatcacg ccggcggtgc tggttgatgt
     3061 cgatgagagc atgctcatca tgcaagaaga gacctttggc ccggtcttac ccattctggc
     3121 agtcgccaac gaagatgaag ccatcgcccg gaccaacacc agcagtttcg gattgggggt
     3181 ctcgatctgg tcgcgcgatc tcaagcgcgc ccggagagtg gcttcccgcc tgaagagcgg
     3241 cgcggcagtg atcaatgatg cgatcgctca ctttgccgta cccctcctgc cttttggcgg
     3301 cgtcgatgat tcaggttatg ggcgaacgca tggcaaggaa ggcttgctgc agttcaccta
     3361 ttcattcgcc attctggagg ggggtccgcc attagcctgg gatattgcca cagtcgcccg
     3421 ccaggcaggg cggtatgatc tggttgcagg catactccgc ctggcttttg gcgtgagctc
     3481 gcgccagcgc ctggagccgg tggtcgagac cctggcgcaa gcccgggagc tgagccataa
     3541 agattggagc actgtccaac gccggcttgc gccatttaag cccgccctgg gggccgctac
     3601 attggccctg ggcgctttcg ccggggctgc tttgttcgcc ggcaaacgcc gcaagcatta
     3661 atttcggtga cagggggtta atctaggatt gtcatttttc cggatcgctg gtatccggga
     3721 tcgaggaggg aatcatgcat gccaggttgg tcagtgtttc cgagatggtc gccattgagc
     3781 gcgccgcggc tgctgccggg cacggctatg cccgaatgat ggaattcgcc gggcggggcc
     3841 tggcagaaga ggttgaagaa gcctatgatt tcttgcgctc cgagggggct ctggggctgg
     3901 tgggctccgg gaataacggc ggtgataccc tggtcgccct ggactggctg gcacagtggg
     3961 gttggacagc gaccgcctgc ctggttcgcc cacgccctgc ggacgatcct ctggttaccc
     4021 gcctgctggc gtctggtggg gaggtattct ctctggaagg cgattcctcc acaactcacc
     4081 tgtacgagcg gctggcgagc tgtgggattg tgctggatgg ggtattgggc accgggatgc
     4141 gcctgccgct caaagttgag gttgcccagg cactggaaca cgtccgtcag gcattggacg
     4201 gtctggaaga tccaccggtg gtggtcgcag tcgattgtcc ttccggtgtg gactgcgaca
     4261 gcggagaagc tgcccccgag accctgcctg ccaacctgac cgtgaccatg gcagcctaca
     4321 aacagggcct gttgaaattt cccgcctatg attatgtggg tgagctgcgc ctggtgggga
     4381 tcggtttacc tcaggatggc gatggcctcg aaccttaccg ttcgctgcag attaccgttc
     4441 cagatatgga cgatgtgcgc gctttgctgc ccctgcgtcc tgcgcaggcc cataagggca
     4501 cattcggcac cgccctgatt gtggccggat ctcaaaatta taccggggca gtcttacttg
     4561 ctggtgaagc tgcctaccgg attgggaccg ggctggtgac cctggccgta accgagatga
     4621 tccagccgat gctggcgggg catatccccg aagcaacctg gctggtgctg ccagaagccg
     4681 gtggaggcgt ctcgccagcg gcggcgcggg tgatcctgga taacctggaa cgggtgactg
     4741 gtttactgat cggtccgggt tttggcctgg cttcccccac tgcagagttt attgcccggt
     4801 ttctggctgg tggtcagtat ccggtccagc tcccggttct gccaccactg gtagtggatg
     4861 cagat
//
LOCUS       DATMXK010000129         2413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_98041, whole genome shotgun sequence.
ACCESSION   DATMXK010000129 DATMXK010000000
VERSION     DATMXK010000129.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2413
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_98041"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..768
                     /locus_tag="VN363_02405"
     CDS             <1..768
                     /locus_tag="VN363_02405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83387.1"
                     /translation="ILILSIGIGYSAFEDTGNSLANLRISRLFLGQPALWEQTAALGE
                     IVANIFHLNLQQVIRFLPGVFGAVAGIILLLLAWFASLLSRRQNGRAKISFGYWAVII
                     FLSVGAIFTPTKVFSGGYHTYACSGNVIRNQEVIGEHLASRIPPGSTVYWRGRLSAVP
                     LLYLPEVQIFPAQINGDYTIYTGGNSERLEKFGFWNEELARQWASEADTILIARHYYS
                     GWLKDHVNAGQFDELEPTPQSAHCEPDAQIRIFRRKQ"
     gene            complement(793..1425)
                     /locus_tag="VN363_02410"
     CDS             complement(793..1425)
                     /locus_tag="VN363_02410"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ83388.1"
                     /translation="MLEIVTFVLGPVQTNAYLVADPDTGAAVAIDPAWDGGSIVLEAQ
                     KRGWHIGSIWLTHAHFDHLGGAGQVADLSSPPPPVALHPADYPLWRAQGGAAWFGIHI
                     DPGPEPTIDLAHAQILHLGGFDFEVRHTPGHTPGHIVLVCAAAGVVFCGDVIFQGSIG
                     RTDLPGGDYDTLIQSIREQILSLPDETRLLSGHGPETTVGEERLENPYLN"
     gene            complement(1433..2185)
                     /locus_tag="VN363_02415"
     CDS             complement(1433..2185)
                     /locus_tag="VN363_02415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83389.1"
                     /translation="MPASRVVKKNYLVSLIFAIGVLLSFACISSTSISPPAEIGEPTR
                     AVEPLPIIETPANTSMPAIQEPRLITLEWPARLRVGDSDTIRLTLEVDETGGITPTAL
                     NAGNETRGERVNIPDLYDTHNVMVEARLDIAGLTYTPTSVVIEPMQPGQAVIFFWSVQ
                     PQQVGKYRGTVWLHLHYVPKTPAGQEARMALSAQVVEIEAINFLGLSGNAARVTGWVG
                     AALGSIISLDRLYDFVIRLIKRSRLKPPQQAG"
BASE COUNT          551 a          623 c          681 g          558 t
ORIGIN      
        1 atattaatcc tttcaatcgg gattggatat agcgccttcg aagataccgg taattcactg
       61 gcaaatttac gcatttccag attgttcctg ggacagccag cgctgtggga gcaaactgcg
      121 gcgctgggtg aaatcgtcgc caatattttc catctcaatt tacagcaagt gatccggttc
      181 ttgccaggcg tttttggggc tgtcgcgggc attatcttgc tactgttggc ctggtttgca
      241 agtcttttgt cccgccgcca aaatggacga gccaaaattt cctttggata ttgggcggtg
      301 atcatttttt tgagtgtggg cgcgatcttt acaccgacaa aggtctttag cggtgggtac
      361 cacacttatg cttgtagtgg gaatgtcatc cgcaatcaag aagtcattgg ggagcatctc
      421 gcgagccgga tcccgcctgg ctcgacggtt tattggcggg ggcggctttc ggctgtccca
      481 ctcttatacc ttcctgaagt acagattttc ccggctcaga tcaatgggga ctatacaatc
      541 tataccggcg ggaattccga gcgcctcgaa aaatttggtt tttggaatga agagctggcg
      601 cgacagtggg cgagcgaggc cgatacgata ttgatcgccc ggcattatta ttctggctgg
      661 ctgaaagatc acgtgaatgc cgggcagttt gatgagcttg agcctactcc ccaatcagcc
      721 cactgcgaac cggacgctca aattcggatc tttcgcagaa aacagtaagg ttaccccgta
      781 atttagaccg gatcaattca gatacggatt ctccagccgt tcttctccaa ccgttgtctc
      841 cggtccatgt cctgataaca agcgggtttc atctggcagg ctgaggatct gctcgcggat
      901 gctttggatc agggtatcat agtcaccgcc aggcaggtcg gtgcgcccga tactgccctg
      961 aaagatcaca tccccacaaa atacgacccc tgctgcggcg caaaccagga cgatatggcc
     1021 tggggtatgg ccgggggtat ggcggacttc aaaatcgaat ccccctaaat gcaggatctg
     1081 ggcatgagcc aggtcgatcg ttggctcagg acccggatcg atgtgtattc caaaccaggc
     1141 agctccaccc tgggcgcgcc agagtgggta gtccgccggg tgcaaggcta ccggtggagg
     1201 tgggctgctg agatccgcga cctggcctgc accaccgaga tgatcgaaat gtgcatgggt
     1261 cagccagata ctgccaatgt gccagccgcg cttctgggct tctagaacga tactaccgcc
     1321 atcccaggcg ggatcaatgg cgactgccgc gcctgtatct ggatcggcaa caagataggc
     1381 attggtttgg acagggccga gaacgaaagt gacgatttct agcatgaagt aattatcctg
     1441 cctgttgtgg gggtttgagg cgcgagcgtt tgatcaatct gattacgaaa tcgtagagcc
     1501 tgtccaggct gatgatcgag cccagagcgg ctcccaccca gccagtaact cgggcggcgt
     1561 tgccgcttaa gccaagaaaa ttgattgctt caatttctac cacctgggcg gagagcgcca
     1621 tacgagcctc ctgcccggct ggcgtcttgg gtacataatg caaatgcagc cagacggtgc
     1681 cgcgatattt cccgacctgt tgaggttgga cactccaaaa gaagatcacc gcctgcccag
     1741 gctgcatcgg ctcaatcaca acgctggtag gcgtataggt caatccggcg atatccagcc
     1801 gggcctcgac cataacatta tgcgtatcat agagatccgg gatattaacc cgctcgccgc
     1861 gtgtttcgtt ccctgcattc aaagcggttg gggtgatgcc gcctgtttca tctacttcca
     1921 gggtcaggcg gatggtgtcg gaatccccga cacgaaggcg cgccggccat tccagagtga
     1981 tcaagcgtgg ctcctggatg gctggcatcg aggtattggc cggggtctca ataattggca
     2041 ggggttcgac tgcacgggtt ggctcgccta tctcagctgg gggtgaaatc gaagtgctgg
     2101 atatgcaggc aaacgacagc agtacgccga ttgcgaaaat gaggctaacc agataattct
     2161 tcttcacgac tcgactggct ggcatcagag aactgcccag agagatagca gggaggtagg
     2221 cgtgggtatt tgcatattct ctggtgctac aggaatagaa agcctatcat ggatggatgg
     2281 ccacaaaccc cataccagca agcctatcga aaccaccagg atgacgattc ctacgagctg
     2341 acgaaaccgg cggatattca ccgttcaatt atagttgatc ggaggacagg agtcacatcc
     2401 gaaaactgat atc
//
LOCUS       DATMXK010000130         2051 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_37544, whole genome shotgun sequence.
ACCESSION   DATMXK010000130 DATMXK010000000
VERSION     DATMXK010000130.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2051)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2051)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2051
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_37544"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..653)
                     /locus_tag="VN363_02420"
     CDS             complement(<1..653)
                     /locus_tag="VN363_02420"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWQ83390.1"
                     /translation="MDVSVLIVNWNVRDLLESCLDSLYNEQHPFSCEVWLVDNASTDG
                     SVEMLQARFPQVHVIQNQSNPGFAQGNIQAYAQSTGRYVLMLNPDTRLHPGALDKLYE
                     YLESNPKAGGVGPDLRNPDGSRQMGCYPFPTLSREFWRLLHLDRIKAYGIYPVDQWDS
                     ATSRRVDVIQGACLLLRRSALEQVGFLDPDYFMYTEEVDLCYRLHRAGWLISWLPAA"
     gene            complement(684..1673)
                     /gene="nadE"
                     /locus_tag="VN363_02425"
     CDS             complement(684..1673)
                     /gene="nadE"
                     /locus_tag="VN363_02425"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560402.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HWQ83391.1"
                     /translation="MQFHKNILDIDAAAEVERIVDFLRSQVRQGMRKQGGVLGISGGI
                     DSSVVLGLCVLAFGPERVIPLMLPEKDSDPESVQLAERLSDHFGVKPVLEVITPVLDG
                     FGCYPRRDEAIRRVFPEYDPSLGYKSKIVLPNNLLEGDTLNVFKVVIVTPDGEEKSHR
                     LTPRDFNQIVAASNFKQRTRMAMLYYHAELHNYAVIGTGNKNEHAQGFFVKYGDGGTD
                     IRPIIHLYKTQVYQLAEYLEIPEDIRRRPPTSDTYSAHSTQEEFFFRLPFRTMDLLWY
                     AQEHGISEAETARVMDMEVVQVQRAFADFTRKQRTTEYLRLPMLELDGVLQPE"
     gene            complement(1660..>2051)
                     /locus_tag="VN363_02430"
     CDS             complement(1660..>2051)
                     /locus_tag="VN363_02430"
                     /inference="COORDINATES: protein motif:HMM:NF012936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="asparagine synthase-related protein"
                     /protein_id="HWQ83392.1"
                     /translation="TEKYLLKKMARQWLPDEIWKRHKQPYRAPIHRSFFNEDTQDYVI
                     DLLSPESINRNGLFNASAVQQLVEKIRRGVRLSETDDMALAGILSTQLIHDRFIDHFR
                     LAPPIADPDDVKVVRKSIGQGVDYAVS"
BASE COUNT          482 a          579 c          529 g          461 t
ORIGIN      
        1 tgcgcggctg ggagccagct gatcagccag ccggcgcgat gcaagcggta gcacaggtcc
       61 acttcctcgg tatacatgaa gtagtcagga tctaaaaatc caacctgttc aagggcactc
      121 cggcgcagaa gcaggcacgc cccctggatc acatctaccc tccgggaggt agcggaatcc
      181 cattgatcta ccggatagat tccatatgcc ttgatccggt ccaggtggag caatcgccag
      241 aactcccggg ataaagtcgg aaatggatag cagcccatct gccgggagcc atccggattg
      301 cgcagatcag gaccgacgcc acctgctttc ggattggatt ctaaatattc atagagctta
      361 tccagcgcgc cgggatgcag ccgtgtatct ggattgagca tcagtacata gcgtcccgtg
      421 ctctgagcat aggcctggat attcccctgc gcaaaaccgg ggttgctctg attctggatg
      481 acgtgaacct gagggaagcg cgcctggagc atttccacac tgccgtccgt ggaggcatta
      541 tccaccagcc agacttcgca gctgaaagga tgctgctcgt tataaagcga atcaaggcag
      601 ctttccagca agtcgcgtac attccagttg actatcagaa ccgaaacgtc catcaatcct
      661 ggactcccag atcaagaccc gttttattcg ggttgaagta ccccatccag ctccagcatc
      721 ggcaggcgta aatattccgt cgtgcgctgt ttgcgcgtaa aatcagcaaa cgcccgctga
      781 acctgaacca cttccatatc catcacccgg gcagtttctg cttcacttat cccatgctct
      841 tgagcatacc agagcagatc catcgtccga aaaggcaggc ggaagaagaa ttcttcctgg
      901 gtgctgtgag cgctgtacgt gtctgaagtg ggtgggcggc ggcggatatc ttccgggatc
      961 tccagatatt ctgccagctg atagacctgg gtcttataga ggtgaatgat cgggcggata
     1021 tcggtgccgc cgtctccata cttaacaaaa aatccctgag catgctcatt cttgttgcct
     1081 gtcccgatca cggcgtaatt atggagctcg gcatgataat acagcatcgc catgcgggta
     1141 cgctgcttga aatttgaggc agccacaatc tggttgaaat cgcgcggcgt cagccggtga
     1201 gatttctctt caccgtcggg ggtgacgatg accactttga atacattgag cgtgtcgcct
     1261 tccaggagat tattgggcag gacgatcttg gatttgtatc ccagggaggg atcatactcc
     1321 ggaaacacgc gccggatcgc ttcgtcccgg cggggataac aaccaaaacc atccagcacc
     1381 ggggtgatca cttccaggac cggtttcacg ccaaaatgat cagacagccg ttctgcaagc
     1441 tgaacacttt caggatcaga atccttctcc ggaagcatca gcggtatcac ccgctcggga
     1501 ccaaaggcaa gcacgcacaa acccaataca accgaagagt caatcccacc actgattccc
     1561 agtactccac cctgcttgcg cattccctgg cgaacctggc tgcgcaagaa atctacaatc
     1621 cgctctactt ctgcagcggc gtcgatatcc aggatgtttt tatgaaactg cataatccac
     1681 tccttggccg attgatttac gcacaacttt gacatcatcg gggtctgcaa tcggcggcgc
     1741 aagcctgaaa tggtcgataa aacggtcatg gatgagctgg gtcgataaga tccccgccag
     1801 agccatatcg tctgtctcgc tcaaccgtac cccgcggcgg attttttcaa ccagctgctg
     1861 gacggcgctg gcgttgaaca gcccgttgcg gttaatcgat tccggcgaca acaaatcaat
     1921 cacgtaatcc tgggtatctt cattgaagaa actgcggtga atcggcgccc ggtatggctg
     1981 tttgtggcgc ttccagatct catccggcag ccactgcctg gccatctttt tgagtaagta
     2041 cttctcggtc a
//
LOCUS       DATMXK010000131         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_14724, whole genome shotgun sequence.
ACCESSION   DATMXK010000131 DATMXK010000000
VERSION     DATMXK010000131.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14724"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..783
                     /locus_tag="VN363_02435"
     CDS             <1..783
                     /locus_tag="VN363_02435"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWQ83393.1"
                     /translation="VYISLPNQLHAEWTIKALEAGIHVLCEKPFAVTLEDVDSMIAAS
                     QRSGCVLAEAFMYRHHPQTKLLDERIQRGDLGDLLAIRGVFNFLIRGGWNIRLAPELG
                     GGSLWDVGTYPVSLAQYIFQSAPDWVSASQRLGPTGVDLTFTGLLHYPGDRTAQIMSS
                     FETPYYAWADIVGTQGRMVLDQPFTGQDENTVLRFYPKDGEMQEIPVPDQYLYLGEVE
                     DMHSAVLDGQPPYLRLSETRDHIRTILALYEAARTHRIVHLE"
     gene            857..2485
                     /locus_tag="VN363_02440"
     CDS             857..2485
                     /locus_tag="VN363_02440"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase family protein"
                     /protein_id="HWQ83394.1"
                     /translation="MIPMRSKPTPIYPPASRPARKSRPNGQAARPRPANGSAPQTLVE
                     RLAPYSFWVLLLAAFAGLFFILAGALGFFAYYQVTERIYPGVHAGSIDLGGMQVPDAA
                     VAIHQVYNLDRRIVLDDGLQPRSLSPSELGISVDAVATAEAAYATGHRKDLFFRFGAM
                     LTGLTEGVSITPVIQFDEARAWQTMADISAQITVPARDAAIQFQDDQLVALPASPGYT
                     LNHAETLAPILLSPANVLSSGYLKAVLLPLSPAVTDAAPQLEAARAFLDAPHRIEAFD
                     PIRNQGQAWEIERSLLAGWLAVSNQAGAVQLDLSVPAVEAHLANLSAQLGDNRRLEPA
                     QDAPRVVSDLLAGQVPALRIYHSPSSYTVQPGDTLLRIAWQVGMPFWIIAQANPGLDI
                     DHLTLGQTLVLPSLDEMLPLPVVPGKRIVMSIGQQRMEIFENGEKLHKFVISTGIDRS
                     PTQPGIFQVQTHDLSAYASVWDLTMPHFLGIYEAWPGFMNGIHGLPTLSNGRRLWANI
                     LGKPASYGCIILDLNNAEWLYNWAEDGVVVEITR"
BASE COUNT          470 a          846 c          696 g          517 t
ORIGIN      
        1 gtctatatca gcctgcccaa ccagctccat gccgaatgga ccattaaagc cctcgaagcc
       61 gggatccacg tgttatgcga gaagccgttt gccgtcacgc tggaagatgt tgattccatg
      121 atcgccgcca gccagcgctc gggctgtgtg ctggcggaag ctttcatgta ccgccaccac
      181 ccccagacaa agctgctgga tgagcgtatc cagcgcggtg atctgggtga cctgctcgca
      241 atacggggtg tatttaattt cttgatcagg ggtggctgga atattcgcct ggctccagag
      301 ctgggcggcg gctcgctctg ggatgtcggt acctaccccg tcagcctggc gcagtacatc
      361 ttccaatccg ccccggactg ggtcagcgcc tcccagcgcc tgggaccgac cggcgtggac
      421 ctgaccttca ccggcttgct ccattatcct ggcgatcgca ccgctcagat catgagctca
      481 ttcgaaacgc cctattatgc gtgggcggac atcgtcggca cgcagggccg gatggtgctc
      541 gaccagccct ttacagggca ggatgaaaac acggtcctgc gtttctaccc caaagacggc
      601 gagatgcagg aaatccccgt ccccgaccag tacctgtatc tgggtgaggt tgaggatatg
      661 cactctgccg tgctggatgg gcagccgcct tacctccgcc tgagtgaaac tcgcgaccat
      721 atccggacga tccttgccct gtacgaggct gcccgcaccc accggattgt gcatctggaa
      781 tagcttagct tcaagggtac caggacatgc aggaacgttc ggggaactaa ttgctgttat
      841 tggcgttaat ttaataatga tcccgatgcg ctcaaaaccc acccccattt acccgcctgc
      901 ctccagacct gcccgaaaat cccgtcctaa tggtcaggca gcccgcccgc gcccggcgaa
      961 tggatccgct ccccagacgc tggttgagcg cctggcgccg tattccttct gggtcctcct
     1021 cctggcagcc ttcgcgggct tattttttat ccttgccggt gccctgggat tcttcgccta
     1081 ttaccaggtc accgagcgga tatatcctgg cgtgcatgcc ggcagtatcg acctgggcgg
     1141 catgcaggtc cccgacgcgg ccgttgccat tcaccaggtc tacaatctgg atcgccggat
     1201 cgtgctcgat gatggcctgc agccgcgctc gctttcccca tccgagctgg ggatttccgt
     1261 cgatgccgtt gccaccgcag aagcggctta cgccaccggg caccgcaaag atctcttctt
     1321 tcgctttgga gccatgctca ccggtcttac agaaggcgtc tccataaccc cggtaatcca
     1381 gttcgatgaa gcccgcgcct ggcagaccat ggcggatatt tctgcccaga tcaccgtacc
     1441 cgcccgggat gcggctatcc aatttcagga cgaccagctg gttgccctgc ccgcctcccc
     1501 cggctacacc ctcaaccacg ccgaaaccct ggcgcccatc ctgctttcac ccgccaacgt
     1561 cctcagcagc ggttacctca aggcagtctt gctgcccctt tcccccgccg tcacagatgc
     1621 cgcaccccag cttgaagctg cccgagcctt cctggacgcc cctcaccgga tcgaagcctt
     1681 cgacccgatc cgtaatcagg gccaggcatg ggagatcgag cgcagcctgc tggcaggctg
     1741 gctggccgtc tccaaccagg ctggcgccgt tcaactggac ctgtcagtcc cggcggtgga
     1801 ggctcatctc gccaatctca gcgcccagct cggcgataac cgccggctgg aacccgccca
     1861 ggacgctccc cgggtcgtct cagacctgct ggccgggcag gttcccgccc tgcggatcta
     1921 ccacagccct tcaagctata cggtacagcc cggggatacc ttgctgcgca ttgcctggca
     1981 ggtcgggatg cccttctgga taatcgccca ggcgaatccc ggactggaca tcgaccatct
     2041 caccctgggt cagaccctgg tcctgcccag cctggacgag atgctgccgc tgccggtcgt
     2101 cccgggcaag cgcatcgtga tgagcatcgg ccagcagcgt atggaaatct tcgagaatgg
     2161 cgagaagctg cacaaattcg tgatcagcac cggcatcgac cgctctccga ctcagcccgg
     2221 tatcttccag gtccagaccc acgatctaag cgcctatgcc tcagtctggg acctgaccat
     2281 gccgcatttc ctgggtatct atgaagcctg gcccggcttc atgaacggga tccacggcct
     2341 gcccaccctc tccaatgggc gccgcctgtg ggcgaacatc ctgggcaaac cggcttccta
     2401 cggctgcatc atcctcgatt taaacaatgc cgaatggctg tataactggg ccgaagatgg
     2461 ggttgtggta gagattactc ggtaagtggg gatataaccg cagatttcgc agatgggcgc
     2521 agataagat
//
LOCUS       DATMXK010000132         2207 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_117150, whole genome shotgun sequence.
ACCESSION   DATMXK010000132 DATMXK010000000
VERSION     DATMXK010000132.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2207)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2207)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2207
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117150"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..218)
                     /locus_tag="VN363_02445"
     CDS             complement(<1..218)
                     /locus_tag="VN363_02445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83395.1"
                     /translation="MIIIDGHQDLAWNMLTFGRNYTRSAAETRRLEAGQAVEVQGGIT
                     LLGWPDYQRGQVAVIFATLFAAPARRSGG"
     gene            complement(249..1322)
                     /gene="mutY"
                     /locus_tag="VN363_02450"
     CDS             complement(249..1322)
                     /gene="mutY"
                     /locus_tag="VN363_02450"
                     /inference="COORDINATES: protein motif:HMM:TIGR01084.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="A/G-specific adenine glycosylase"
                     /protein_id="HWQ83396.1"
                     /translation="MSQFPFTEHLLNWYRNNARQLPWRGQRDPYAIWVSEIMLQQTRV
                     ETVIPYFERWIEHFPTVRSLAEAPLQEVLAVWEGLGYYSRARNLHKAARQIWEQGGVL
                     PSTTSELRTLPGVGRYTAGAIASIAFGENAAALDGNIRRVLARLFDVEEPARSLKGEA
                     RLWELAESNLPSGEAGDYNQALMDLGATLCTPHDPDCIQCPLTAFCQAYQLGIQEQRP
                     VNLPKPSIPHYQVAAAVLEREGRYLITQRPFQGLLGGLWEFPGGKQEPAETLPECLGR
                     ELQEELGVRVRVGEQLNVYKHAYTHFRVTVHAFHCFWQQGEPEPQLLQVNDLAWAELS
                     DLAKYPMGKVDRQIASHLSEGAG"
     gene            complement(1319..1621)
                     /locus_tag="VN363_02455"
     CDS             complement(1319..1621)
                     /locus_tag="VN363_02455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83397.1"
                     /translation="MAVKLLLTWDILPGREQDYFEFVVRDFIPGMQHLGMEPGEAWFT
                     MYGNQPQILVAAQMNTMAALQNILVSPDWDNLTKRLLDYVENYTYKVVPARGGFQM"
     gene            complement(1637..>2207)
                     /locus_tag="VN363_02460"
     CDS             complement(1637..>2207)
                     /locus_tag="VN363_02460"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ83398.1"
                     /translation="DHTVAGYEALKLRGDIVTVSHDAPGHNFVSAVKGRAHVITGPGE
                     FEIGGVFITAVQTNGSHKDTPDEPRNTLYVFDYDGITVAHLGDLQKVPNQAEIEALGN
                     VNIVLVPVGGGGGLTAAKAVEVVSLLEPGIVIPMHYSTPDGGTNLSPVGKFLKEMGLS
                     DIEPLPSLKVTQSAIPEETRVVILNYEKS"
BASE COUNT          529 a          631 c          533 g          514 t
ORIGIN      
        1 cccccactcc gccgcgcggg ggctgcaaac agggtcgcga aaatgaccgc cacctgccca
       61 cgttggtaat caggccagcc caataaggtt atgcccccct gaacttccac cgcttgcccg
      121 gcttccagcc ggcgagtctc ggctgccgag cgcgtgtagt tgcgtccaaa agtcagcata
      181 ttccaggcca gatcttgatg cccatcaatg ataatcatgt tctggctgcc ctgcgattga
      241 gataatcttc acccagcacc ttcagacaaa tgggatgcaa tctgacggtc tactttaccc
      301 atggggtatt ttgccaggtc agacagctcc gcccatgcca ggtcattcac ctgtaatagc
      361 tgcggttctg gttcaccctg ctgccagaaa caatggaatg catgcacggt gacgcggaag
      421 tgggtataag catgcttgta tacgttcaac tgctcgccga cccggactcg aacacccagc
      481 tcctcctgta attcgcgtcc caggcattct ggaagggttt cagctggctc ttgtttgccc
      541 ccgggaaact cccataggcc acccaataat ccctggaaag gtcgctgggt gatcaaatag
      601 cgtccttctc gctccaaaac tgcagcagct acctgataat gcggaatcga tggtttaggc
      661 aggttgactg gacgctgttc ctggataccg agctggtaag cctggcaaaa cgcggttaat
      721 gggcattgaa tacaatcagg atcatgcgga gtgcatagtg tagccccgag atccatcaaa
      781 gcctggttgt aatcgcctgc ctcgcctgaa ggtagattgc tttctgccag ctcccacaag
      841 cgggcttccc ctttgagtga gcgcgccggc tcttcgacat caaaaaggcg cgctaggacc
      901 cgcctgatat tcccatccag agcagctgca ttctcgccaa aggcaatcga tgcaatcgct
      961 ccggctgtgt accggccaac ccctggtaat gtgcgcagct cactggtagt tgatgggagc
     1021 acgcctccct gctcccaaat ttgacgagcg gccttgtgca aattgcgcgc ccggctgtaa
     1081 tagcctaacc cttcccatac tgccaacact tcctggagag gagcttcagc caggcttcgc
     1141 accgttggaa aatgttctat ccagcgctca aagtaaggga tcacagtctc cacccgagtt
     1201 tgttgcagca taatctccga aacccagatt gcataagggt cgcgttgacc acgccaggga
     1261 agctggcgag cattgttacg ataccagttg agaagatgtt cagtgaaggg aaattgactc
     1321 acatttggaa tccgccgcgc gccgggacaa ccttatacgt ataattctcc acataatcta
     1381 agaggcgttt tgtcaggtta tcccaatccg gtgagacgag gatattttgc aacgcagcca
     1441 tggtattcat ctgggcagcc acaaggattt gaggctgatt tccatacatt gtaaaccagg
     1501 cttcaccggg ttccattcca aggtgctgca tgcccgggat aaaatcacgg actacaaatt
     1561 caaaataatc ctgttcgcgc ccaggtagga tatcccaggt caagagaagc ttgacagcca
     1621 tatcggttct ccttattcag gatttttcat aattcaagat aacaacccgg gtttcctcag
     1681 ggatagcgct ctgagtcacc ttcaaggatg gcaggggctc aatatcgctc agccccattt
     1741 ctttcaagaa ctttccgacc ggagacaggt tggtgccacc gtcaggagtg ctgtaatgca
     1801 tcgggatgac aatccctggc tctaagaggc tgaccacctc tacagctttg gcagctgtaa
     1861 gccctcctcc tccgcctacc ggaactaaaa caatattcac attgcccaat gcttcgatct
     1921 cagcctggtt cggaactttc tggagatcac ccagatgggc cacagtgatt ccgtcgtaat
     1981 cgaatacata cagcgtattc cgcggttcat cgggagtatc tttatgactg ccattcgtct
     2041 gcacggcagt aataaaaacc ccaccaatct caaattcacc gggacctgta atgacatgag
     2101 cgcgcccttt caccgccgat acaaaattat gcccgggggc gtcatgactc acagtgacaa
     2161 tatcgccgcg taattttaac gcttcataac cagctacagt atgatcg
//
LOCUS       DATMXK010000133         6198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51779, whole genome shotgun sequence.
ACCESSION   DATMXK010000133 DATMXK010000000
VERSION     DATMXK010000133.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6198
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51779"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..208)
                     /locus_tag="VN363_02465"
     CDS             complement(<1..208)
                     /locus_tag="VN363_02465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83399.1"
                     /translation="MKYSSQAHRPTVLLGAILVLITLSLACVRSGQDGSNSLWSISGL
                     RQPTPTQTIASPLLPGSFVPTSPPG"
     gene            290..2035
                     /gene="ftcD"
                     /locus_tag="VN363_02470"
     CDS             290..2035
                     /gene="ftcD"
                     /locus_tag="VN363_02470"
                     /EC_number="2.1.2.5"
                     /inference="COORDINATES: protein motif:HMM:TIGR02024.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030409 - glutamate formimidoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0030412 - formimidoyltetrahydrofolate
                     cyclodeaminase activity [Evidence IEA];
                     GO_process: GO:0019556 - histidine catabolic process to
                     glutamate and formamide [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate formimidoyltransferase"
                     /protein_id="HWQ83400.1"
                     /translation="MRTLAVHLRRAASCMHQARPDGLPGSPWRYNNDALPRIEKAKTL
                     IWQRIQRKNTYKEIKLRMTTSLVECIANFSEARRPEVVEAIRQAIEATAGVAILDQHS
                     DLDHNRTVLTFVGPPEEIGEAAFRAIARAAELIDLDQHQGEHPRIGASDVVPFVPIQG
                     VTMPECVELARRLGERVGRELEIPVYLYEEAATRPERVNLENIRRGQYEALKLEIATQ
                     PEREPDFGPRRVGPAGATVIGARQPLVAFNVYLTSEDVSIAEKIAKAVRNSSGGLRFV
                     KALGLLVEGRAQVSMNLTHFRKTPLARVVELVRREAQRYGVGIHHSELVGLIPQEALV
                     EAARWYLQLDQLEPEQILEQRLAAVQAESPAAVKGGEETGGGESFLDALAAGTPAPGG
                     GSAAAYAGAAGAALVAMVARLTIGKKKYAAVEAQMQAVLIRAEELRAVLQQAVEQDSE
                     AFKAVMEAYRLAKETLEQQAERSKAIEGAMLAAARAPLKVAGMAVKVLELASQVIAEG
                     NSNAISDGASAAAMARAALNGSGLNVRINVNSLADPQSGQALVAELEGLEQQALHLEA
                     GIRQVLQVRAGLVLP"
     gene            2032..3741
                     /locus_tag="VN363_02475"
     CDS             2032..3741
                     /locus_tag="VN363_02475"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWQ83401.1"
                     /translation="MSSLYRFNQSQFRNGLPGLVMLVLVILLAGCQAGGAGTPAVSSP
                     AAATAVQMPTGVVLPASPTPLPPTLAPTPVPRIWLADYLPPAITESFVLPPGFQMTSE
                     REAAQVRLEVGSDTAAANWVYALAAPFPTIRDDISLTDLQAIWRGAAPVGLETISFVL
                     VEDIRALFAALWGEPGAGVVEVLPRAQLVERAWEIRTAWTLLPFEALEPRWKVLSVDG
                     SSPLWKTFDPEIYPLSVGFAWSGEPELVSYLNRTVWQGKQPLTNRDPQKLTTVVMTGV
                     TALVRATAFTMHRNGITYPGQDVGPILREADFAHVSNEIPFTEDCPPADPSQAGLVFC
                     TRPEYIGLLEDIGTDVVELTGDHFGDWGPEAMLYTLELYKERGWIVYGGGANREEARQ
                     AQLIEHNGNKLAFIGCNAKGGGYATASESRPGAVACDFEWMHGEITRLKAEGYQVIST
                     FQHFEYYSYAAQPNQIADSEGMSQAGAAIVSGSQAHQPQAMEFYQGGFIHYGLGNLFF
                     DQYYMGVPTSQGFLDRHVFYDGRYLSTELIGIYFVDYARPRLMTAEERVELLEAVFKA
                     SGW"
     gene            3775..5529
                     /locus_tag="VN363_02480"
     CDS             3775..5529
                     /locus_tag="VN363_02480"
                     /inference="COORDINATES: protein motif:HMM:NF021127.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CapA family protein"
                     /protein_id="HWQ83402.1"
                     /translation="MRLRGVLFLAILLISACSPGAQQPPLVAGGEQQGSVQPATRTPF
                     QPLAAEPSGGAAPTLALPAPAPTPTAMPPTPTLGAAAQLPVWVDPLLPAAFRASLSLP
                     AELAALPGESSAESGVLRLEAGGSRLVSRWIYALVAPFPTQVENVSAETLQNAWSGAG
                     QSPWGSLPLLLDPGTQRMLSAAWGAPAEGAVRILASDELLSEAWKRSPAWAIIPFEAL
                     EPRWMVLKVEGQSPLQPTFDLSTYPLTLPVSLTGDEQLAEAIYALYGPQSAAPLAQAA
                     NWRSDHLTTLAMTGVTALVRATAFTMERQGNQYPARDIGPLLNSAGLTHISNEIPFAR
                     NCPFPDPVQAGLRFCSDPGYIALLEAVGTDIVELTGDHFQDWGEEAMLLTLDLYRERN
                     WAYYGGGENLEDGRKAFLVEHNGNRLAFIGCNAKGGGYAQAAANHPGAVKCDFDYLTK
                     EIARLRSEGYLPIATFQHFEYYTYKAQPDQVKDFRALAEAGAVIVSGSQAHQPQAMAF
                     EGSAFVHYGLGNLFFDQYDISLATRQGFIDWHVFYEGRYISTELVPILFVDYARPRFM
                     NGDEKQQLYNAVFSASGW"
     gene            5574..6128
                     /gene="dcd"
                     /locus_tag="VN363_02485"
     CDS             5574..6128
                     /gene="dcd"
                     /locus_tag="VN363_02485"
                     /EC_number="3.5.4.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010543264.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008829 - dCTP deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0009394 - 2'-deoxyribonucleotide metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dCTP deaminase"
                     /protein_id="HWQ83403.1"
                     /translation="MGLKPDHWIRKMAREYNMIEPFADSQVRNGVISYGVSSYGYDIR
                     VADEFKIFTNVYSAVVDPKNFNPQSMVDFKGDICVIPPNSFALARTIEYMRIPRKVLT
                     VCLGKSTYARCGIIVNVTPFEPEWEGYVTLEISNTTPLPARIYANEGIAQVLFFEADE
                     ECEVSYADKKGKYQKQQTIVLPKL"
BASE COUNT         1288 a         1660 c         2019 g         1231 t
ORIGIN      
        1 tccctggagg gctggtgggc acgaaacttc ccggaagaag tggcgaggca atggtttggg
       61 tgggggttgg ctggcgcagg cctgagatgc tccagagtga gtttgagcca tcctgaccgg
      121 agcggacaca ggccagactg agcgtgataa gaaccagaat ggcccccaac aatacggtgg
      181 ggcgatgagc ctgtgaagaa tatttcatga acacgcctgt gcgctaacct taaagaattc
      241 taccaataaa tgatcgaccg gtacagggtg cagcgggagg caaacggagg tgcgtacact
      301 ggccgtgcac cttcgtcgtg ccgcttcgtg tatgcaccag gcgcggcctg acggcttgcc
      361 gggcagtccc tggcggtata ataacgacgc gctgcccagg atcgaaaagg ctaaaacctt
      421 gatctggcag cgcatccaaa gaaaaaatac ttataaggag ataaaactgc gcatgaccac
      481 atctctggtc gaatgcatag caaatttctc ggaggctcgc cgcccagagg tggtggaggc
      541 gatccgccag gcgatcgaag cgactgccgg ggtggcaata ttagaccagc actcagacct
      601 ggaccacaac cgcactgtgc tgacctttgt aggcccgccg gaagaaattg gggaagctgc
      661 ctttcgggcg attgccagag cagctgaatt aatcgatctg gaccaacacc agggagaaca
      721 cccacgcatt ggggccagcg atgtggtgcc gtttgtgccg atccaggggg taacgatgcc
      781 ggagtgtgtg gagctggcgc gcaggctggg agagcgggtc ggacgagagc tggaaattcc
      841 ggtttactta tatgaggagg ccgccacgcg gccagaacgg gttaaccttg aaaatatccg
      901 gcgcgggcag tacgaggctc tgaagctgga gattgccacc cagccagagc gcgagccgga
      961 ttttgggccg cggcgtgtag gacccgcagg agcaacggtg atcggagctc gccagccgtt
     1021 agttgccttc aatgtgtatc tgacgagtga ggatgtcagc attgccgaaa agatcgccaa
     1081 agcggtgcgc aattcatccg gcggtctgcg tttcgttaaa gccctggggc tgttggtgga
     1141 agggcgagcg caggtttcga tgaatctgac ccatttccgg aagacgcctc tggcgcgggt
     1201 ggtggagctg gtccggcggg aggcgcagcg ctatggggtt ggaatccatc acagcgagct
     1261 ggtgggtctg atcccgcaag aggcgctggt tgaagcggcg cgctggtatt tacagcttga
     1321 ccagctcgaa ccagaacaaa tattagagca gcgcctggca gcggtccagg cggagagtcc
     1381 tgccgcggta aagggcggtg aggaaacagg tggaggggaa agctttttgg atgctctggc
     1441 agcaggtaca ccagcgccag gcgggggctc ggcggcggca tacgccgggg cggccggagc
     1501 ggcactggtg gcgatggtgg cgcgcctgac gatcggaaag aagaaatacg ccgcagttga
     1561 agcacagatg caggcggtat taatccgggc ggaggagctg cgggcggtct tgcagcaggc
     1621 ggtagagcag gattccgaag catttaaggc tgtgatggag gcttatcgcc tggcgaaaga
     1681 gaccctcgag cagcaagctg agcggagtaa ggcaattgag ggggccatgc tggcggctgc
     1741 ccgggcgccc ctgaaagtag cgggaatggc tgtgaaggtg ctggagctgg ccagccaggt
     1801 gatcgcagaa ggcaactcga acgcgatcag tgatggcgcc agcgcggcgg cgatggcgcg
     1861 ggcggcgttg aatgggtcgg gtttaaatgt gcggatcaat gtgaacagcc tggcagatcc
     1921 ccagagtggg caggcgctgg tcgccgagct ggagggcctg gaacagcagg ctttgcatct
     1981 ggaagccggc atccgccagg ttctgcaggt gcgtgcagga ctggtattac cttgagttct
     2041 ttataccggt tcaaccagag ccaatttcgg aatgggctgc ctgggctggt tatgctggtt
     2101 ctggttatcc tgctggcggg ctgccaggca gggggtgccg gtaccccggc tgtgagcagc
     2161 cccgcagcgg cgacggctgt tcagatgcca acgggggtgg tcctgccggc cagcccgacg
     2221 ccgctgccgc caaccctggc gccgacgcct gtgcccagga tctggctggc ggattatctc
     2281 ccgccagcga tcacggaaag ctttgtattg ccacccggat tccagatgac ctccgagcgt
     2341 gaagcagccc aggtgcggct ggaggttggc tcagatactg ctgcagcgaa ctgggtgtat
     2401 gccctggcag cgccatttcc aaccatccga gatgatatca gcctgaccga tttgcaggcg
     2461 atctggcgcg gcgcggcgcc ggtcgggttg gagacgatct cgtttgtatt ggttgaggat
     2521 atccgggcat tgtttgcggc cctgtgggga gagcctggcg cgggcgtggt tgaagtgctt
     2581 ccccgcgcgc agctggtaga gcgggcctgg gaaatacgga ccgcctggac cctgcttcct
     2641 ttcgaagcgc ttgagccgcg ctggaaagtg ctcagcgtgg atggcagctc acctttatgg
     2701 aagacattcg atccggagat ttacccctta tcggttggct ttgcctggtc gggcgagccg
     2761 gagctggtct cctatttaaa tcggacagtc tggcaaggga agcagcccct gaccaaccgc
     2821 gatccacaga agctgaccac tgtggtaatg actggcgtaa ccgccctggt gcgagcgaca
     2881 gccttcacaa tgcaccgcaa tgggatcact tacccggggc aggatgtagg cccgatcctg
     2941 cgggaagccg attttgcgca tgtcagcaac gaaattcctt tcaccgagga ctgcccgcca
     3001 gcggatccca gccaggcggg gctggttttc tgcaccaggc cggaatatat cggattgctg
     3061 gaggatatcg ggacggatgt ggtggagctg accggcgacc attttgggga ttggggtccg
     3121 gaggccatgc tctacacgct tgaattatac aaggagcggg gctggattgt atatgggggg
     3181 ggcgctaacc gggaagaagc ccgccaggcg cagctgattg agcacaacgg caacaagctg
     3241 gcgttcatcg gctgtaatgc caaaggcgga gggtatgcta ctgccagtga gagccgcccg
     3301 ggagccgtag cgtgtgattt tgaatggatg catggcgaga ttacccgctt gaaagcggaa
     3361 ggctaccagg tgatctccac tttccagcat ttcgagtact atagctacgc agcgcagccc
     3421 aatcagattg ctgattcaga ggggatgagc caggcaggtg cagctatcgt cagcggcagc
     3481 caggctcacc agccccaggc gatggaattt tatcagggcg gcttcatcca ttatgggctg
     3541 ggcaacctgt tcttcgacca gtattatatg ggggtgccca ccagccaggg ctttttggac
     3601 cggcatgtat tttatgacgg gcgctacctg agcacggagc tgatcgggat ctactttgtc
     3661 gattatgccc gcccgcgatt gatgactgcg gaagagcggg tggaattact ggaagctgtc
     3721 ttcaaagcga gtgggtggta aagactggca gacaaggtac cagggaggtc aggtatgcgg
     3781 ttgagaggcg ttctattttt agcaattcta ttgatctctg cctgctcgcc tggcgcgcag
     3841 cagccgccat tggtggccgg cggcgagcag cagggaagcg tacaacccgc gacacgaacc
     3901 ccctttcagc cgctggcagc cgagccatcc ggcggggctg caccaacgct ggcgctgcca
     3961 gcgccagcac cgacgcccac cgctatgcct ccgacgccaa ctctgggggc tgcggctcag
     4021 ctgccggtat gggtagatcc tttattgcct gctgctttcc gggcgagtct gagcctgccg
     4081 gccgagctgg ctgctctgcc aggtgagagc tcagccgagt caggtgtgct gcgcctggaa
     4141 gctggcggct cccgcctggt cagccgctgg atatatgctc tggttgctcc cttcccgacg
     4201 caagtcgaaa acgtttcggc ggaaactctg cagaatgcct ggagtggagc cgggcagagt
     4261 ccctggggaa gcctgccgct gctgctggac cccggcacgc agcgtatgct cagcgccgcc
     4321 tggggagcgc cggcagaggg cgcggtgcgg attctggcga gtgacgagct gctgagtgaa
     4381 gcctggaagc gcagccctgc ctgggcgatt atccccttcg aggcgcttga gccgcgctgg
     4441 atggtattaa aagttgaagg ccagtcgcct ttgcagccta catttgatct cagtacctat
     4501 ccgctgaccc tgcctgtttc cctgaccggc gacgagcagc tggcggaggc gatctatgca
     4561 ctttacgggc cgcaaagcgc ggcgcctctg gcacaggcag cgaactggcg cagcgaccac
     4621 ctgaccaccc tggcaatgac cggcgtcacc gcattggtgc gggcgacggc tttcaccatg
     4681 gaacgccagg ggaaccaata cccggcacgc gatatcggcc ctctgctcaa cagcgccggg
     4741 ctgacccata tcagcaacga aatccccttt gcgcgcaact gccccttccc ggaccccgtt
     4801 caggcgggac tgcgcttctg cagcgacccc ggctatatag ctttgctgga agcggtggga
     4861 acagacatcg tggagctgac cggcgaccac tttcaggatt ggggcgagga agccatgctg
     4921 ctgaccctgg acctgtaccg ggagcggaac tgggcttact acgggggtgg ggaaaatctg
     4981 gaagacgggc gcaaggcctt tctggtggaa cacaacggca accgcctggc gtttatcggc
     5041 tgcaatgcca aaggcggcgg ctatgcccag gcagcagcca atcacccggg agcggttaag
     5101 tgcgattttg actatctgac gaaagagatc gcccggctgc gcagcgaagg atatttaccc
     5161 atcgccacct tccaacattt cgagtattac acctacaaag cccagccaga ccaggtgaag
     5221 gattttcggg cgttggcaga ggcaggcgcg gtgatcgtca gcggcagcca ggcccaccag
     5281 ccccaggcga tggcctttga ggggagtgca tttgtccatt atgggctggg gaacctgttc
     5341 tttgaccagt acgatatcag cctggcaacc cgccagggat ttatcgactg gcatgtgttt
     5401 tatgagggcc gctacatcag caccgagctg gtgccaatct tatttgtaga ttatgcccgg
     5461 ccgcgcttta tgaacggcga tgaaaaacag caattataca atgctgtatt ttcagccagc
     5521 ggctggtaaa attctgccgt taatcagact ttgttttttt tgcgaggagc agcatgggac
     5581 tgaaaccaga tcattggatc cgcaagatgg cacgcgaata taacatgatc gagccattcg
     5641 ccgatagcca ggtacgcaac ggggtgattt cgtacggggt gtcgtcttat ggttatgaca
     5701 tccgcgtggc agacgagttt aagattttta ccaatgtcta ctcggcagtt gtcgacccga
     5761 agaacttcaa cccgcagtcg atggtggatt ttaagggtga tatctgcgtc atcccaccga
     5821 actccttcgc cctggcgcgc accatcgaat atatgcgcat ccctcgtaag gtgctcacgg
     5881 tttgtctggg gaaaagcact tatgcccgct gtgggatcat tgtcaatgtg acgccttttg
     5941 agccggagtg ggaaggctat gtgactctgg aaatttccaa taccacaccc ctgccagccc
     6001 gcatctacgc caatgagggg atcgcccagg tgctcttctt cgaggccgac gaagagtgcg
     6061 aggtttcata tgcggataag aagggcaaat accagaaaca gcagacaatc gtcttgccca
     6121 agctataaat taaagccaga accccgcctg tgaaataagc actctgggcg gggttttttg
     6181 ttttaatccg ctttgtta
//
LOCUS       DATMXK010000134         3305 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_48448, whole genome shotgun sequence.
ACCESSION   DATMXK010000134 DATMXK010000000
VERSION     DATMXK010000134.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3305)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3305)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3305
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48448"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(80..1420)
                     /gene="murJ"
                     /locus_tag="VN363_02490"
     CDS             complement(80..1420)
                     /gene="murJ"
                     /locus_tag="VN363_02490"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWQ83404.1"
                     /translation="MTPPQTTANRQIARAAGTVMFAFMLSTLTGLLRWVLVANAFGTS
                     AEMEAFSAANRVSETLYYLVAGGALGSAFIPTFTGLLAKDQRRKAWEMASAITNLVLL
                     VLIFASLVAAIFAPAIVRHILAPGFTSDPYKFDLTVSLVRLMLPSAIIFGVSGLVMGI
                     LNSHQIFLFPALAPSMYALGMIFGVLFLSPRMGIYGLAWGVLLGAALHLLVQVPVLVR
                     QGGRYTATLGLRLAEVGEVARLMGPRLLGVAVVQLNFWINVRLASAMPAGSVNGVTYA
                     FTLMLMPQTVIAQSIATAAMPTFSAQYARGQISEMRNSLSATLRGVLLLALPASAGLV
                     LLRFPLVATLYQRGEFDAQSTELVAWALLWYGVGLVGHSVVEILARAFYALHDTRTPV
                     LVGAGAMGLNVIFSFLFSAWFERLGWMPHGGLALANTAATGLEMLGLLIAMRSR"
     gene            1635..2546
                     /locus_tag="VN363_02495"
     CDS             1635..2546
                     /locus_tag="VN363_02495"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWQ83405.1"
                     /translation="MVQLRLEFEAHSNYALPVLFPGRVDELVTAGMDRMIRHWKLPEL
                     PEAGSPQLIREVVAHANSVNSLAFTPDRRSLLSASTDASICIWSWPDLQLIHTLSGHK
                     KTVAGLAVAPGGSLAASASYDSQVMLWNIPTGELSGILKGHRRNVTCVAFSPDGRTLA
                     SSGLGEDICIWDIPSQTIQKKLPGHKDAVGTLAYTPDGRYLLSIDNAGVLRLWAAAGL
                     QLKREIQLPKSRMGSFAFHPNGEICAIGAEHRILLGEWLSGEIIAEIPFKPSAVAGIS
                     FSPDGKSLAAGFADRKVRLWDLGSTVG"
     gene            2549..2938
                     /locus_tag="VN363_02500"
     CDS             2549..2938
                     /locus_tag="VN363_02500"
                     /inference="COORDINATES: protein motif:HMM:NF013222.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MGMT family protein"
                     /protein_id="HWQ83406.1"
                     /translation="MGFTSPPNPTQFNQQVWTLVRQIPHGEVASYGMIAALMPLPPNV
                     TPEDFRAWGARWVGGAMARCPADVPWQRVVNSQGKISLPRGGPYERQQGLLQEEGVVF
                     GPGGRIDLERFGWAGPNLPDQAIIPSE"
     gene            2948..>3305
                     /locus_tag="VN363_02505"
     CDS             2948..>3305
                     /locus_tag="VN363_02505"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005934810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWQ83407.1"
                     /translation="MSADVEPTRCAWPGTDPLYVRYHDEEWGVPVHDDRKLFEFLILE
                     GAQAGLSWITILRKRPAYQLAYAGFDPEQVAQYDEAKIAELLANPGIVRNRLKIQASI
                     GNARAFLKVQEEFGSFD"
BASE COUNT          739 a         1000 c          930 g          636 t
ORIGIN      
        1 gcaagaaagc ggccatcccg gcggtagcga gacccccctg cagtagaacc tggaggattt
       61 gcgaccctgc cagtccaccc taccggctgc gcatggcgat cagcagcccc agcatttcga
      121 gtccggtggc cgcagtattg gccagggcca ggcctccatg gggcatccat cccagacgct
      181 caaaccaggc tgaaaacaaa aaactgaaga tcacattcaa gcccattgcc cctgctccta
      241 ccaggaccgg agtgcgggta tcgtgcagcg cataaaaagc ccgcgccagg atctcgacca
      301 cggagtgacc gaccaggccc acgccgtacc ataacaaagc ccaggcaacc agttctgtgg
      361 attgggcgtc aaattcaccg cgctggtaaa gtgtcgccac cagggggaag cgcagcagca
      421 ccaggccggc gctggcaggc agtgccagga gcagcacccc gcgcagggtc gctgagagcg
      481 aattacgcat ctcgcttatc tggccgcgcg catattgggc ggaaaaagtc ggcatggctg
      541 ctgtggcaat cgactgggcg atcaccgttt gcggcatgag catcagggtg aaggcgtagg
      601 tcaccccatt aacgctgcct gctggcatcg ccgatgccag gcgcacattg atccagaaat
      661 tcagctgcac gaccgcgacg cccagcaagc gcggccccat cagccgggct acctcgccca
      721 cttccgccag gcgcaaaccc agcgtcgctg tgtagcgccc tccctggcgg accaggaccg
      781 gcacctgcac caacaggtgc agtgctgctc ccagcagcac accccacgcc aacccgtaga
      841 tccccatcct cggcgacagg aagagcacgc cgaagatcat ccccagggca tacatcgatg
      901 gcgccagcgc cgggaacagg aagatctggt gagaattcaa gatgcccatc accagcccgc
      961 tgaccccaaa gattatcgcc gagggcagca tcaggcgcac cagggagacg gtcaggtcga
     1021 atttataggg atcgctggtg aaacccggcg ccaggatatg gcgcactatc gcgggggcaa
     1081 agatcgctgc caccaggctg gcaaaaatca aaaccagcag caccaggttg gtgattgccg
     1141 aagccatctc ccaggctttc cggcgctggt ctttcgccag cagaccggtg aaggtgggga
     1201 taaaagccga acccagggca ccgcctgcca ccagatagta caaagtctca gagacccggt
     1261 tagcagccga gaaagcttcc atctctgccg atgtgccaaa agcattggcg acgagaaccc
     1321 agcgcagcag cccggtaagg gtgctgagca taaacgcaaa cattaccgtg ccggccgcac
     1381 gggcaatttg acgattggca gttgtctgtg ggggagtcac tgctaaatat tataatccgg
     1441 acttcccatc taccctgtca attagcccac ccgtgaaaaa ttcaccttca cttagaacaa
     1501 ttgttcaggt ataatgggct gcattccaat ttatgataca atccaacctt acgcgggcct
     1561 aaatcgtatt taattggagc ccactcctcc gaatattcaa cgttctccga ataacctccg
     1621 cgaaggagct gtgcatggtt cagttacgcc tggaatttga agctcattca aattatgccc
     1681 tcccagtttt gtttccgggc cgcgtcgatg agctggtcac tgcaggaatg gaccggatga
     1741 tccggcactg gaagctgccg gagctgccag aggctggatc gccgcagctg atccgggaag
     1801 tcgtcgccca tgcgaacagc gtcaacagcc tggctttcac tccagaccgg cggtccctgc
     1861 tttctgcatc cacagatgcc agtatttgca tctggtcctg gcctgacctg cagctgatcc
     1921 atacgctttc cgggcataaa aagacggtcg ccggcctggc tgtagcgcct ggcggctcac
     1981 tggctgccag cgcatcgtat gattcacagg tgatgctctg gaatatcccc acaggcgagc
     2041 tgtccggtat tttgaaaggc cacagacgta atgtcacctg cgtggcattt agcccggatg
     2101 gcaggaccct ggcttcttcc gggttgggtg aagatatttg catctgggat attccttccc
     2161 agactatcca aaagaaacta cccggtcata aagacgccgt cggaactctg gcttacactc
     2221 cggatgggcg ctatttgctt tccatcgaca atgccggcgt cctgcgttta tgggccgcgg
     2281 caggtctgca gcttaaacgt gagatccagc ttcccaaaag ccggatgggt tcattcgctt
     2341 ttcatcccaa cggagagatc tgcgccatcg gtgcggaaca ccgcatcctg ttaggagaat
     2401 ggctatcggg tgaaatcatc gcagaaattc cattcaaacc ttctgcggtg gcaggcatat
     2461 ccttctctcc cgacggcaaa agcctggctg ccggatttgc agaccgcaaa gtacgcctct
     2521 gggatctcgg atccacggta ggataatcat gggttttacc tcgcctccca atcccaccca
     2581 gtttaaccaa caggtctgga ccctggtgcg ccagattccc cacggagagg tcgcctcgta
     2641 cggcatgata gcggcgctaa tgcctctgcc ccccaatgtg accccggaag atttccgtgc
     2701 ctggggtgcc cgctgggtcg gcggggccat ggcgcgctgt ccggcagatg taccctggca
     2761 gcgcgtggta aacagccagg gtaagataag cctgccgcgc ggcggacctt acgagcgcca
     2821 gcaaggcttg ctccaggaag aaggtgttgt tttcggtccg ggtgggcgga tcgatctgga
     2881 acgcttcggt tgggccgggc ccaacctgcc agaccaggca atcatcccat ccgaatagag
     2941 actatctgtg tctgctgatg ttgaacctac ccgctgcgcc tggccaggaa ccgacccatt
     3001 gtatgtccgg tatcacgatg aagaatgggg cgtcccggtg catgatgacc ggaagctgtt
     3061 cgagtttttg atcctggaag gggctcaagc aggcctgagc tggatcacca tcctgcgcaa
     3121 acgcccggca taccagctgg cgtatgccgg ttttgatccc gagcaggtag cccaatatga
     3181 tgaggcaaaa atcgccgagc tgctggcgaa tcccggcatc gtccgcaacc ggctcaagat
     3241 ccaggcttcg atcggcaatg cccgagcctt cctgaaagtt caggaggaat ttggtagttt
     3301 cgatg
//
LOCUS       DATMXK010000135         3768 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_13156, whole genome shotgun sequence.
ACCESSION   DATMXK010000135 DATMXK010000000
VERSION     DATMXK010000135.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3768)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3768)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3768
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_13156"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..640)
                     /locus_tag="VN363_02510"
     CDS             complement(<1..640)
                     /locus_tag="VN363_02510"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ83408.1"
                     /translation="MRTATRLVVVGQTGDCCAVCIRWIARTVRRVINMTRYAIVLDTR
                     RCIGCQSCTVACRVHNDLPLDMIYNPVTTVGPTGVFPNVHMVHIPLLCMHCANPPCVE
                     CCPTGASQQRADGIVWVDEQKCVGCKACVMACPYGARIPNHEKGIVQKCDFCMDRVDM
                     GKEPYCVATCHQRARIFGDLDDETSEVFRLVNRENVVRLLPELGTEPHAFYIM"
     gene            complement(543..2783)
                     /locus_tag="VN363_02515"
     CDS             complement(543..2783)
                     /locus_tag="VN363_02515"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ83409.1"
                     /translation="MSKNIDLEQEGIQVKKSCCFFCHPNCGVLAYVKDGRVLEIEGDP
                     DNPTNQGGLCCRGNIALKHLDHPARVNYPLKRIGQRGSGEWERISWDQALDEIAARLA
                     QIREEYGAEAVATAGGTQRTDDWARRRFMNLYGSPNGFHNAHLCWIPTFMVETAIYGW
                     CPFETDLGNSRCLVQWGHNPGASGMPEMRGITDLKLKGMKLIVIDPKFTETAAKADLW
                     LPLRPGSDLALALAWLNVIIWEGLYDAAFIENCCIGFNELAEHVREYTPEWAAPLTWL
                     DAELIRESARMYATNKPGNIQWGVAVDQSGTPAGTAMHARALLRAVTGNLDAPGSDLL
                     TGPSLEFVTDEEMEANEWLPDEQKAKQIGSDRYKLTTWPGYGRIAELTKEVWGKAPTA
                     EWMCEAHPPSVFRAILTGEPYPVKALLVSAANPINSYGESLQVLEALRKVDFLVTCEY
                     WMTPTSMLSDYVLPIAGALERPTFHSVYGCSDFVIATQRAIQPMYERRTDYEFWRGLA
                     IRMGQEEMWPWENLEEANYYRIHPLGYDVSSYDEFVEKIFYHFPEREYYKYGRLGFAT
                     PSRRVELVSSTLAELGYPSMPSYIGPAENEIDDPELAEKFPLVLTTGSGFMPYHHSEH
                     FQIKELRFLRHDPYMDINPATAAGLGIQDGDWVWIETQRGRIKQKANLTQAIHPRVIV
                     TQRGWWYPEREACEPELGGCLESNTNVLTSVADEDCDPIGGSWANRGLLCRVYKVDSS
                     NGKEGH"
     gene            complement(2799..3590)
                     /locus_tag="VN363_02520"
     CDS             complement(2799..3590)
                     /locus_tag="VN363_02520"
                     /inference="COORDINATES: protein motif:HMM:NF024118.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ83410.1"
                     /translation="MGAQIIFLGTGAGCGVPAFFCTCVACREASSEPRYQRGRCAMLI
                     RGEYNTLIDAPPDLRTQLLREHVHQIDHFVLTHGHYDHSGGLGELEFYVRVGRQEAIP
                     AYMTPGTGEWLHTAYGFLEDCLSIQTIDAGWQFDQDGVAYTGLEVTHAPGTLGLLLET
                     PGGRRTAYLPDTGPLPASTKDQLQGIDTLILGATFWGRNWMPEDHLSVEEAVRIGLSL
                     NVKKLYLTHLSMHHDIPVTNRELEAYLRTFGDHLHLAYDGLCIEI"
BASE COUNT          829 a         1096 c         1018 g          825 t
ORIGIN      
        1 ccataatata aaaagcgtgg ggttcggtgc ccagctctgg cagcaagcgc accacgttct
       61 cccggttcac cagccggaaa acttcgctgg tttcgtcatc caggtctccg aagattcggg
      121 cgcgctggtg acaggtcgcc acacaatacg gctcttttcc catgtcaacc cgatccatgc
      181 agaaatcgca tttttggacg atacccttct catggttggg gatacgcgcc ccgtagggac
      241 aggccatcac gcaagcctta cagcccacgc acttttgctc gtcgacccaa acgatgccat
      301 cagcacgttg ctgagaagcg cctgttggac agcattcaac acacggtgga ttagcacaat
      361 gcatgcacag gagcggtata tgtaccatat gcacattcgg aaagaccccg gtagggccta
      421 cagtggtcac cgggttataa atcatatcca ggggaaggtc gttatgaact ctacaggcta
      481 cagtgcaaga ttgacagcca atacaacggc gggtatccag gacgattgca tagcgcgtca
      541 tattaatgac cctccttacc gttcgagcta tccaccttat acacacggca cagcaatccc
      601 cggtttgccc aactaccacc aatcgggtcg cagtcctcat ctgcgacact ggtcagcacg
      661 ttagtgttcg attccaggca gccgcccagc tcgggctcac aggcctcccg ctccgggtac
      721 caccagccgc gctgggtgac aatcacccgt gggtgaatgg cctgcgtcag gttggccttc
      781 tgcttgatgc gcccgcgctg ggtctcgatc cagacccaat caccgtcctg gatgcccagc
      841 ccggctgctg tggccggatt gatgtccata tatggatcgt ggcgcaggaa gcgcagttct
      901 ttgatctgga agtgctcaga gtgatggtaa ggcatgaacc cgctgcccgt ggtcagcacc
      961 agcgggaatt tctcggccaa ctcaggatca tcgatttcat tttcagctgg cccgatatag
     1021 gagggcattg atggataacc caactcagcc aatgtgctgg acaccagctc cacccgcctg
     1081 gaaggtgtag caaagccgag gcgaccatac ttgtaatatt cccgttccgg gaaatggtag
     1141 aagatctttt caacaaactc gtcataactg ctcacatcat agcctaatgg atggatgcga
     1201 taatagttgg cttcttccag gttttcccat ggccacatct cttcctggcc catacggata
     1261 gccagaccac gccaaaactc gtaatccgtc cgccgttcat acatgggctg gatcgcacgt
     1321 tgagtggcaa tcacaaaatc tgagcagccg taaacgctgt ggaaggttgg gcgctccaac
     1381 gctcctgcga tcggcagcac ataatcggaa agcatcgagg tgggggtcat ccaatattca
     1441 caggtaacca gaaaatctac cttgcgtaag gcttccagaa cttgcagact ttcgccatag
     1501 gaattgatcg ggttggcagc tgagaccagt aaggccttca ccgggtaagg ctcaccggtc
     1561 aggatagccc tgaagaccga aggtgggtgg gcttcgcaca tccattctgc ggttggagct
     1621 ttgccccaga cctctttggt cagctccgcg atcctgccat agcctggcca ggtggtgagc
     1681 ttatagcggt ctgacccgat ctgcttggcc ttctgctcgt ccggcagcca ttcgttggct
     1741 tccatctcct catcggtcac aaactccagg ctgggaccgg ttaacaggtc tgagccgggt
     1801 gcatccaggt tgccggtaac ggcccgcagc aaggcgcggg catgcatggc ggtacccgcc
     1861 ggtgtgccag actgatcgac cgccacaccc cattggatgt tgccgggttt gttggtggcg
     1921 tacatgcggg cgctttcccg gatcagttct gcatccagcc aggtgagcgg agctgcccac
     1981 tccggcgtgt actcgcggac atgttctgcc agctcattaa acccaatgca gcagttctcg
     2041 ataaaggctg cgtcatacaa cccttcccag atgatcacat tcagccaggc cagcgccagc
     2101 gccaggtccg agccgggacg caaaggcagc cagagatccg ccttggctgc cgtctcggta
     2161 aattttgggt cgatgacaat cagcttcata ccttttagct taagatcggt aataccacgc
     2221 atttcaggca tccctgaagc gcccgggtta tgaccccatt gcaccaggca gcggctgttg
     2281 cccaagtcag tctcaaacgg gcaccagccg tagatggccg tctccaccat aaaggtcggg
     2341 atccagcaca gatgggcgtt gtgaaaaccg ttggggctgc cgtacaggtt cataaaacgc
     2401 cggcgagccc agtcatcggt gcgctgcgtg cctcctgcgg ttgctaccgc ttcagccccg
     2461 tattcctcac ggatttgtgc cagcctggca gcaatttcat ccagggcttg atcccaggaa
     2521 attcgttccc attccccgga gccgcgttgg ccaatccgtt ttaaggggta attcacgcgc
     2581 gccgggtgat ccaggtgttt gagcgcgatg tttccccggc aacataaccc tccctgattg
     2641 gtcgggttat caggatcgcc ttcgatctcc aggacgcgcc cgtctttgac gtaggcaagc
     2701 accccgcagt tgggatggca aaaaaaacaa caggattttt tgacctggat accttcttgt
     2761 tcgagatcga tgtttttgct caaagttacc ctccaggatt agatttcgat acacaatcca
     2821 tcataagcca ggtgtagatg atccccaaat gtccgtaaat acgcttccag ctcccggttc
     2881 gtgacaggaa tatcgtggtg catcgagaga tgggtcagat agagcttctt cacgttgagg
     2941 ctcaatccga tccgaaccgc ctcttcgacc gacaggtgat cttccggcat ccagttcctg
     3001 ccccaaaagg tagcgcccag gatcagggta tcgatgccct ggagctggtc cttggtagag
     3061 gccggcaatg gcccggtgtc ggggaggtag gcggtccgcc ggccgcccgg cgtttcgagc
     3121 agcaatccga gtgtgcccgg tgcatgggtg acttcaagcc cggtgtaggc tactccgtcc
     3181 tggtcaaact gccaaccagc gtcaattgtc tgtatggaaa gacagtcttc caggaaacca
     3241 taggcagtgt gcagccattc cccggtgcca ggggtcatat acgcggggat agcttcctga
     3301 cgcccgaccc gcacgtagaa ctccagctca cccagcccac cggaatggtc gtagtgtccg
     3361 tgggtcagga cgaaatggtc gatttgatga acatgttcac gcaggagctg ggtgcgcaga
     3421 tcgggcgggg cgtcgatgag agtattgtat tcgcctcgga ttaacatagc acaacggccg
     3481 cgctggtaac gcggctcact ggaagcttca cggcatgcaa cgcaagtaca gaagaacgct
     3541 ggcacaccgc agcccgcacc tgtccccagg aaaatgatct gcgcacccat atcaaacacc
     3601 ttttaccacc ataaccggta cctgtaagta ttgtagaaga taccggctgc ctacggaata
     3661 ctcttccggg taaaattcca ctagacaaat ggattatatt tgccagcagc ccatcctatt
     3721 agccagagat ggttcactcg cctggttttt tagggggtgc gggcggcg
//
LOCUS       DATMXK010000136         4328 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_50093, whole genome shotgun sequence.
ACCESSION   DATMXK010000136 DATMXK010000000
VERSION     DATMXK010000136.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4328)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4328)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4328
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50093"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1158
                     /locus_tag="VN363_02525"
     CDS             <1..1158
                     /locus_tag="VN363_02525"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWQ83411.1"
                     /translation="MVSYPSYENNRMARIIPAYYYEQLVRDPSNPLLNHAVGDVLPAG
                     SVFKLVTAVGSLNEGVVTPEQVIDTPPELIVTERNYLSTNDSGLARRFVDWNEAGFGQ
                     LDFVHGLANSSNVYFYKLGGGYEDEVPEGLGICRLGTYAEALGYGDAPNVGLPDEQNG
                     LIPNPTWKRVTHGESWTTGDTYIAGVGQGYVLATPIQVLISAAIVANDGKYMQPTLVR
                     EILDEEGNVVEPFEPELRWDLTQDAVIQEYGETTIRGCEPLVGQTTTVAPWVFEKVQE
                     GMRLAVTEGTLANPTIGFGPDSLDIAAAGKTGTAEYCDIYANAKNRCIPGAWPSHAWT
                     VAYAPFENPEIAVVAFVYNGEEGSSVAGPIVAQTLQAYFELKSIDNALQAP"
     gene            1263..1946
                     /gene="minC"
                     /locus_tag="VN363_02530"
     CDS             1263..1946
                     /gene="minC"
                     /locus_tag="VN363_02530"
                     /inference="COORDINATES: protein motif:HMM:TIGR01222.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004857 - enzyme inhibitor activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinC"
                     /protein_id="HWQ83412.1"
                     /translation="MEITTKIQIKGIREGLLVTLGDAPWEQAAEALIEHLDQQREFLK
                     GARMALDVGNHVLKAADLGQLRNQISDREVTLWAVISNSPTTERTAQTLGLETRLAKT
                     KPEPAAQDTRYQDGDPAILVHRTLRSGYSLQNPGHVVILGDVNPGAQIISGGDVIVWG
                     RLRGMVHAGAGGNESAVICALDLSPTQLRIAGAIAITPQRKLKPQPETARIVDGQVVA
                     EPWNPKKRE"
     gene            1962..2792
                     /gene="minD"
                     /locus_tag="VN363_02535"
     CDS             1962..2792
                     /gene="minD"
                     /locus_tag="VN363_02535"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559399.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="septum site-determining protein MinD"
                     /protein_id="HWQ83413.1"
                     /translation="MDYSGNGKQMNGKVITVTSGKGGVGKTTLTANLAAALAADGQKV
                     VCIDGDIGLRNLDVVLGLENRIVYDLVDVVEGRCRLRQAMIRDKRLPELYLIPAAQTR
                     DKNAVSPSDMVRLANELRPEMDWILIDSPAGIERGFRNAIAPADIVLVITNPEVSAVR
                     DADRIIGLIEAEEKGPARLVINRIKPAMVKRGEMLSTEDVLELLAIELIGLIPDDESV
                     VTSSNRGNPIAIDGKTKAAQAFRNIARRLNGHKVPFLELQEDGNLFGRLARLLRPGGN
                     "
     gene            2793..3059
                     /gene="minE"
                     /locus_tag="VN363_02540"
     CDS             2793..3059
                     /gene="minE"
                     /locus_tag="VN363_02540"
                     /inference="COORDINATES: protein motif:HMM:TIGR01215.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0000918 - division septum site selection
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division topological specificity factor
                     MinE"
                     /protein_id="HWQ83414.1"
                     /translation="MNSFFDRLFRKEPNSASQAKERLKLVLIHDRTDMAPGTLEMMKD
                     ELIAVISRYVDIDPEAVRIDMAQEGREQRLIADIPLRPPKRRRQ"
     gene            3147..4277
                     /locus_tag="VN363_02545"
     CDS             3147..4277
                     /locus_tag="VN363_02545"
                     /inference="COORDINATES: protein motif:HMM:NF013279.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsW/RodA/SpoVE family cell cycle protein"
                     /protein_id="HWQ83415.1"
                     /translation="MNEKNSISWRHFDFLLLGAVALLVIFGITMIRSAVAGNIELEEL
                     DVVTRQIIFAVGGFVVVLVVASIDYRLWASISRTLYLGTAGLLAILTVVGAALFGSAR
                     WFDTGVILIQPSELAKIVLILVLADFFTRNQENLGSIRVVIRSLIMAMGIVIWVILQP
                     NLSTSIVMVVIWFALLWAAGLRVRHLVTVGGSAVLILALLTPILISADIIKEYQLDRV
                     TNFLFPDETASYGESYNIQQALISIGSGGWFGQGYGHGSQVQLRFLKVRTSDFIYSAM
                     AEEFGFIGTILVTLVLLFVIYRCMRAAHLSQDTFGALICYGVATLLTFQAMVNIGVNL
                     NLMPATGLTFPFVSYGGSSLLSSLIGIGLVESVILRHRSLEF"
BASE COUNT          983 a         1088 c         1239 g         1018 t
ORIGIN      
        1 atggtgtcct acccatcgta tgaaaataac cggatggcgc ggatcatccc ggcttattat
       61 tatgaacaac tggtccgcga tcccagcaat cccctcctga atcacgccgt cggcgatgtg
      121 ctgccggcag gctcagtatt caagctggta acagcagttg gctctctaaa cgaaggtgtg
      181 gttacgcctg aacaggtgat agatacaccc cctgagctga tcgtcaccga aaggaattat
      241 ctaagcacga atgactcagg ccttgctcgc cgcttcgtgg actggaacga ggcaggtttt
      301 ggacagctgg attttgtcca cggtctggca aattcgagca atgtgtattt ctacaagctc
      361 ggcggtggtt atgaagacga agttccggaa ggcctgggaa tttgccgcct gggcacgtac
      421 gcggaagctc tggggtatgg agatgctcca aatgttggcc tgcccgatga gcagaatggt
      481 ctgatcccca accctacctg gaaacgtgtg acgcacggcg aaagctggac caccggtgat
      541 acctatattg ccggggttgg tcagggctat gtactggcga ctccaatcca ggtattaatc
      601 tctgctgcga tcgttgccaa cgatggaaaa tatatgcagc ctaccctggt gcgtgagatc
      661 ctggatgaag aggggaatgt ggtcgagcct ttcgaaccgg agctgcgttg ggacctgacc
      721 caggatgccg ttatccagga atatggtgag acgacgatcc gcggctgtga gccattagtc
      781 gggcagacga ctacagttgc gccgtgggtt ttcgaaaagg tgcaggaagg aatgcgtctg
      841 gcagtaaccg aagggacact ggccaaccca accattggct tcggtcccga ttcgctggac
      901 attgctgcgg caggtaaaac cggcaccgct gaatactgcg acatatatgc caatgcaaag
      961 aaccgctgca tccccggcgc ctggccttct catgcctgga ccgtagccta tgcgccgttt
     1021 gaaaatccag aaatcgcggt ggttgccttc gtctataacg gtgaagaggg atcttctgtt
     1081 gccggtccga ttgtcgccca gacactccag gcatactttg agctaaaatc gattgacaat
     1141 gccctgcagg ccccttagac gatggctgtg ctataatcag ccattatctt acggggtaca
     1201 cctgcacagg ataattatta attccccaaa ccaccggcat aaacgcgctc agatagattt
     1261 gcatggaaat aaccacaaag atccaaataa aaggtattcg ggagggcctg ctggtcactc
     1321 tgggagatgc accctgggag caggcggcag aagctttgat cgagcattta gatcagcagc
     1381 gtgaattcct taaaggggcg cgcatggcgc tggatgttgg caatcatgtt ctcaaagctg
     1441 ctgatctggg ccagctgcgg aaccagatct cagaccggga ggttaccctg tgggctgtaa
     1501 tcagcaattc gcccaccaca gagcgcactg cccagacgct ggggctggaa accaggctgg
     1561 caaagaccaa acctgaacca gcagcccagg atacccgcta ccaggatggc gatccggcga
     1621 ttcttgtcca ccgcaccttg cgctcagggt atagcctgca aaaccccggg catgtggtta
     1681 tcctgggaga tgtaaatcca ggagcgcaga ttatctcggg aggggatgta attgtctggg
     1741 ggcgcctgcg cgggatggtg catgccggtg ccggtggaaa tgaaagcgcg gtgatctgcg
     1801 ccctcgatct gtcgcctacc cagctgcgga tcgccggggc gatcgcgatc accccacaac
     1861 ggaaactcaa acctcaaccg gaaacagccc gcatcgtgga tgggcaggta gtggctgaac
     1921 cctggaatcc caaaaaaaga gagtgaagtc aggattattg aatggactat agtgggaatg
     1981 gtaaacaaat gaatgggaag gtcattaccg tgacttcggg gaaaggcggt gtgggaaaaa
     2041 ccaccctgac agccaacctc gccgccgccc tggctgcaga tgggcaaaaa gtagtctgca
     2101 ttgatggtga tatcggcttg cgcaacctgg atgtggtttt aggcctggag aaccggatcg
     2161 tttacgattt agtcgatgta gtcgaaggtc gctgccgcct gcgccaggcg atgatccgcg
     2221 ataaacgcct acccgagcta tatctgatcc cggctgccca aacccgggat aaaaatgcag
     2281 tctctcccag cgatatggtc cgccttgcca atgaactgcg gcctgaaatg gactggattc
     2341 tgattgattc tcccgccggg atcgagcgtg gtttccgcaa tgcgattgcc ccggcagata
     2401 tcgtcctggt gatcaccaac ccggaagtat ccgcggtccg ggatgcggac cggatcattg
     2461 gacttattga agcggaggaa aagggcccgg cgcggctggt gatcaaccgg atcaaacctg
     2521 ccatggttaa acggggcgaa atgctttcca cggaagatgt cctggagctt ttagctatcg
     2581 aattgatcgg cttgatcccg gatgacgaaa gcgtcgtcac cagctccaac cgcggcaatc
     2641 cgatcgcgat tgatgggaaa accaaagctg cccaggcttt ccggaatatt gcccggcggt
     2701 tgaatggtca caaggtgcct ttcctggagc tccaggagga tgggaatttg tttggccggc
     2761 tggcgcgact cctgaggccg ggaggcaact agatgaacag cttttttgac cggttattcc
     2821 gtaaagaacc gaacagtgcc tcccaggcta aagagcggtt aaagctggta ttgatccacg
     2881 atcgaaccga tatggctccg ggaacactcg aaatgatgaa agatgagctg attgcggtaa
     2941 tctcccggta tgtagatatc gatcctgagg ctgtgcggat cgacatggcg caagaaggcc
     3001 gcgaacagcg cttgatcgcg gatattccac ttcgcccgcc aaagcgcagg agacaataag
     3061 gtgatttcca gcgcaggtcg cagatgccag gccagcatgg ggtggcgtgc cgaccaggct
     3121 gccacacaca gcgataattg atatttatga acgaaaaaaa ttcaatttcc tggcgtcatt
     3181 ttgatttctt actcctgggt gcggtcgcgc tgctggttat ctttggcatt acgatgatcc
     3241 gctccgcagt tgccgggaat atagagctgg aagagctgga tgttgtcacc cgacaaatca
     3301 tctttgcggt cggtggattt gttgtggtat tggttgttgc atcgattgat taccgcttat
     3361 gggcttcgat cagccgcacc ttatatttgg ggactgccgg tctgcttgcc attctaaccg
     3421 ttgtgggggc agctctattt ggctcagcgc gctggtttga taccggtgtg atcctgatcc
     3481 agccctccga gcttgcgaaa atcgtcctta ttctggttct ggcagatttc ttcactcgca
     3541 atcaggaaaa tcttgggagt atccgggtgg tgatccgcag ccttatcatg gcaatgggga
     3601 ttgtaatctg ggttatctta cagcccaatt tatccacttc gatcgtaatg gtggtgatat
     3661 ggttcgccct gctgtgggca gcaggactgc gcgtcaggca tctggtaact gttggggggt
     3721 cagcggtgct gatccttgcc ctcctgaccc cgattttgat cagcgctgat attatcaaag
     3781 aataccagct tgaccgggtt accaattttt tgtttcccga cgaaactgcc agctatggtg
     3841 agagctataa tatccagcag gctttgatca gcatcggctc aggtgggtgg tttgggcagg
     3901 gttacgggca cggctctcag gtccagctgc gctttttgaa agtacgtacc tcggatttta
     3961 tctactctgc gatggctgaa gaattcggtt ttataggcac aatcctggtt accctggtgc
     4021 tgttatttgt gatttaccgc tgtatgcggg ctgctcacct ttcgcaggat acttttggtg
     4081 cgttgatttg ctatggagtg gctacgttgt tgacatttca ggccatggtg aatatcgggg
     4141 tgaacctgaa tttaatgccg gctacaggcc tgacattccc ctttgtaagc tacggaggca
     4201 gctcgctgct ctcctcgctg atcggtatcg gactggtaga gagcgtaatc ctcaggcacc
     4261 gctcactcga attttaattt tcgccgcctg ttggcgcgct aactgatcat ctggaacagg
     4321 aacaatac
//
LOCUS       DATMXK010000137         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_139019, whole genome shotgun sequence.
ACCESSION   DATMXK010000137 DATMXK010000000
VERSION     DATMXK010000137.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139019"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..987)
                     /locus_tag="VN363_02550"
     CDS             complement(<1..987)
                     /locus_tag="VN363_02550"
                     /inference="COORDINATES: protein motif:HMM:NF013179.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWQ83416.1"
                     /translation="MAIAPPNLHPGNTLLGYAELYENWLSFVQKHRLNSGVEPFVAHS
                     WMRCYPHLNPQQPLRPSRLSPGLLEAADSNASIRTVGRRVLEDIYEYIEGTNTALMLV
                     NGLGYIIDILGDPDILALAEERGLRQGAIYSEPYVGTNAFAMALAEGVPVRVRGPEHF
                     IQQFHIFSEAAAPIFDISGRLLGAAGLVNLFEEQYPHSLSLAVSAAQIIVTQRTSEQL
                     LADQNQQIAQMNAILSAISEGVMVWSAEGRLLQINEAAEQIVSLPRQAILGKHYRDFI
                     SLPSWLEEAIQERQSMSSVTARVRTGNRPLDLVVSLNFLQNKSGVVNAIVTLH"
     gene            complement(1047..1832)
                     /locus_tag="VN363_02555"
     CDS             complement(1047..1832)
                     /locus_tag="VN363_02555"
                     /inference="COORDINATES: protein motif:HMM:NF018649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1295 domain-containing protein"
                     /protein_id="HWQ83417.1"
                     /translation="MDFVSIYLILGGVIFVLMTLLWLVSLALKNSSIVDIFWGTGFVV
                     VAWIAFLLTPDGYEDRKWLLAILVTLWGLRLSLHILVRNWGKLEDFRYQVWRKEAGAA
                     WWWRSYFKVFLLQGILLWIISAPLLAAQISAQPARLTWLDLLAIPVWLFGFFFEAVGD
                     WQLSHFKANPANKGKVLNTGVWRYTRHPNYFGDATQWWSYYLIALAAGGWWTIFSPLL
                     MTTLLWRVSGASLLEKTLRDEKPGYREYIETTSEFIPWFPRQK"
     gene            complement(1862..2698)
                     /locus_tag="VN363_02560"
     CDS             complement(1862..2698)
                     /locus_tag="VN363_02560"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ83418.1"
                     /translation="MSVRIVTDSTCDLPAEIIARYGIHVVPLYINVGKQSYLDGIDIT
                     RQEFYTRLPIFPTHPTTAVPSIERFQAAYNTLAEEGASEVLSIHISKALSGVVDVARV
                     AAQETSAVPVTVIDSQQLSLGTGFLVETAARMAGAGSSHAEIQAAIADQTRRTHVFAA
                     LDTLEYLKRSGRMSKYLAGIASLLQIKPILTMHRGNPGSERVRTRDRALNRVVKMLAA
                     VGEIERIAIVHTHAPPESISELRARAAGLLPAGEIPVEDITPVIGAHIGPGALGFAVV
                     GS"
     gene            complement(2707..3051)
                     /locus_tag="VN363_02565"
     CDS             complement(2707..3051)
                     /locus_tag="VN363_02565"
                     /inference="COORDINATES: protein motif:HMM:NF025607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4345 family protein"
                     /protein_id="HWQ83419.1"
                     /translation="MDILQILKSIAALATAATGLLAIIKPDAVYGFTGLDANGVRGVS
                     EIRAIFGGLFIGLGLAPFFLGPTAYKMLGIAYLAIAVARIFSIVVDKSYAQSNWISLG
                     IELVLGLILVLK"
     gene            complement(3059..3388)
                     /locus_tag="VN363_02570"
                     /pseudo
     CDS             complement(3059..3388)
                     /locus_tag="VN363_02570"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology.
                     GO_function: GO:0016209 - antioxidant activity [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="redoxin domain-containing protein"
     gene            complement(3401..3532)
                     /locus_tag="VN363_02575"
     CDS             complement(3401..3532)
                     /locus_tag="VN363_02575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83420.1"
                     /translation="MPQVSLNTPAPEFSLPNFRGETVNLSDYYGRSHVLLVFNRGFT"
     gene            complement(3532..4302)
                     /locus_tag="VN363_02580"
     CDS             complement(3532..4302)
                     /locus_tag="VN363_02580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83421.1"
                     /translation="MQDILRQVSVIVTVLATIVVNTLANALPINGLNTGEISDRFEVY
                     FVPAGYVFSIWGLIYIGLIAFTIFQAMPSQRQNPRMRATGWWVSLGGLANIAWIFLWH
                     YEIFPLTILAMAALLITLIITYLRLGIGRTQVSTAEKWAARVPVSIYLGWITVATVAN
                     MTSLLDFLQWDGFGLAPEIWMVIMLAVVLVLTGLMIFTRRDVAYSLVILWALVGIALK
                     HAAVSAVAIPTWITFALVALTLMAALITRKPALKLASL"
BASE COUNT         1061 a         1218 c         1183 g          859 t
ORIGIN      
        1 atgcagggtg acaatggcgt tgaccactcc acttttattt tgcaggaagt tcaggctgac
       61 caccagatca agcgggcgat ttccggtgcg cacccgggca gtcacactgc tcatcgactg
      121 tcgttcctgg atggcttctt ccagccagga aggcagagag ataaaatccc gataatgctt
      181 accaagaata gcctggcgcg gcaggctgac gatctgttcg gcagcttcat tgatttgcag
      241 cagccgtccc tcagcactcc agaccatcac cccttcggag attgcgctca gaatggcatt
      301 catctgggca atctgctggt tctggtccgc caggagctgt tcggaagtac gctgggtcac
      361 aatgatctgc gcggcagaaa ccgccaggct gagactgtga ggatactgtt cctcgaacag
      421 attgaccagc ccggccgctc ccaataacct gccggagata tcgaaaatgg gcgcggctgc
      481 ttcagaaaat atatggaact gctggatgaa atgttctgga ccgcgtaccc ggactggcac
      541 gccttcggca agcgccatcg caaaggcgtt ggtacccaca taaggctcag agtagatagc
      601 cccctggcgc aagccccttt cttctgccag tgccagaata tctggatcgc ccagaatatc
      661 aatgatatac cccaggccgt tcaccagcat cagggctgta tttgtgcctt caatatactc
      721 gtaaatatct tccaacaccc ggcgaccgac cgtgcggatg ctggcattgc tgtcggcagc
      781 ttccaacaga ccgggactga gccggctcgg acgcaatggc tgctgtgggt tcaggtgggg
      841 gtagcagcgc atccaggaat gagctacaaa tggctcgacg cccgaattga gccggtgctt
      901 ctgcacaaag ctcaaccagt tctcgtagag ctcggcatag cccagcagag tattcccggg
      961 atgaaggtta ggcggagcaa ttgccatatc aggttaattg taccgtgaat ttaccggaca
     1021 atgagcctat cgagttgtgt ggcgggttat ttctggcggg ggaaccaggg gatgaattcg
     1081 ctggtcgttt cgatatattc tcgataacca ggtttctcat cccggagggt tttttccagg
     1141 agggaggctc ctgaaacgcg ccagagcaag gtggtcatga gtagcgggct aaaaatcgtc
     1201 caccagccgc cagcagccag agcaatgagg taatacgacc accactgagt ggcatcccca
     1261 aaataattgg ggtggcgcgt atatcgccat acaccggtat tcaggacctt gcctttgttt
     1321 gccgggttgg ctttgaaatg gctgagctgc caatcgccaa ccgcttcaaa gaagaagcca
     1381 aacagccaca ccggaatcgc caggagatca agccaggtga ggcgagcggg ttgggcgctg
     1441 atctgcgccg ccagcagggg agcggagatg atccacagca agatgccttg cagcagaaat
     1501 actttgaagt aactgcgcca ccaccaggct gcgccggctt cctttcgcca gacctggtag
     1561 cggaaatctt ctagcttgcc ccagttgcgg accaggatat gcaaggacag gcgcaagccc
     1621 cacagtgtca ccaggatggc gagcagccat tttcggtcct cataaccatc cggagtgagc
     1681 aagaaggcta tccaggcaac caccacaaaa cctgtccccc agaaaatatc cacgatactg
     1741 gagttcttca aggccaggct gaccagccag agcaaggtca tcaacacaaa aataacgccg
     1801 cccaggatca gataaatcga cacgaaatcc atcgcttgcc ctccactttc gatacaacaa
     1861 cttaagaacc gacgacagca aatccgagag cccccggacc gatatgggcg ccaattaccg
     1921 gggtgatatc ttctaccggg atctcgcctg ctgggagcaa gcctgcagcc ctggcgcgca
     1981 gctcgctgat gctttcgggt ggggcgtggg tatggacaat ggcaatccgc tcaatttctc
     2041 ctacggctgc cagcatctta accacccgat tcagagcgcg atcgcgggtg cgcactcgtt
     2101 cgctgccggg gttgccccgg tgcatggtca ggattggctt gatctgcaag agcgaggcaa
     2161 tcccggccag gtatttgctc atccgcccgc tgcgttttag gtattccaag gtatccaacg
     2221 ccgcaaagac atgggtacgc cgggtctggt cggcgatcgc agcttgaatc tcagcatgcg
     2281 agctcccagc accagccatc ctggcagcag tctctaccag aaaaccggtc cccaggctca
     2341 gctgttggga gtcgatcacc gttaccggga cagcagaggt ctcctgggcg gcgacccgcg
     2401 ccacatctac cacgccgcta agtgccttcg agatatggat ggagagcact tcgctggcgc
     2461 cttcttcagc cagggtgttg taggcagcct gaaaccgctc aatcgaaggc actgcggtgg
     2521 tcggatgagt ggggaatatc ggaagccggg tataaaattc ctggcgcgtg atatcgatcc
     2581 catcaagata actttgcttc cccacattga tatacagggg taccacgtga atcccgtagc
     2641 gggcgataat ttcagccggc agatcgcaag tactatcggt aacgatccgt acagacatat
     2701 ccagcctcac ttaagaacca gaatcaagcc caggacaagc tcaatgccca ggctgatcca
     2761 gttcgattgg gcatacgatt tgtcgaccac gatggaaaat atccgggcaa ccgcaatcgc
     2821 cagataggca attcccaaca ttttataagc ggttgggccc agaaagaaag gggcaagccc
     2881 caggccaata aacagccccc caaagattgc gcgaatctcg gaaacacccc ggacgccatt
     2941 tgcgtccagc ccggtaaagc cgtacaccgc gtcgggttta atgatggcca gaagccccgt
     3001 cgctgcggtc gccagggcgg cgatggattt taagatctgc aagatatcca tatggttcct
     3061 actcacggat gttcagctga tcgaataaag ccaaaagttc accattatcg ggtatatctg
     3121 acatcgagtg cccgtaatga acgaaacgtg ctacaccgtt cttatccacg atcacctgag
     3181 ccggcatgcg ccccagcttg aacaggttga tctcctgccc gtagagcttg agcacactgg
     3241 ctttgggatc aggcaaaccg atgaagggta gatcatgctg ccgccagtaa gctgcaaagg
     3301 cattggcatc ttcagggccc agcacaatca cctgcgcctg gcgtttgata aattccgggt
     3361 aatcctggcg caactgcgcc atgtgccgct ggcagaatgg tcaggtaaag ccgcggttaa
     3421 acaccagcag cacatgggag cgtccataat aatcggacag gtttacagtc tcgcctcgaa
     3481 aattgggcag gctgaactct ggtgcggggg tattcaggga tacttgtggc atcagaggct
     3541 cgctaatttc agcgccggct tgcgcgtgat cagcgcggcc atcagggtca acgccactaa
     3601 cgcgaaggtg atccaggtgg gaatcgcgac tgccgaaacc gcagcatgtt taagcgcaat
     3661 ccctaccagc gcccacagga tcaccaggct gtacgccaca tcccggcggg tgaagatcat
     3721 cagtcccgtg agcaccagaa cgacagccag catgatgacc atccagatct ccggggccag
     3781 cccaaaacca tcccactgca ggaaatcaag caatgaagtc atattcgcca cggttgctac
     3841 tgtgatccag cccagataga tgctgaccgg tacacgcgcc gcccatttct cggcggtgga
     3901 aacctgcgtc cgaccgattc cgaggcgcag gtaagtgatg atcagggtga tcagcaaggc
     3961 agccattgcc agaatggtga gcgggaaaat ttcgtaatgc cacaggaaga tccaggcaat
     4021 attcgccagc ccacccagcg aaacccacca gccggtggca cgcatgcggg gattctggcg
     4081 ctgagagggc atggcctgga agatggtgaa ggcgatcagc ccgatataga tcagccccca
     4141 gatggaaaac acatacccgg ccggaacaaa atatacctca aagcgatcgg agatctcccc
     4201 ggtattgagg ccattaatcg gtaaggcgtt cgccagggta ttgacgacga tcgtggccag
     4261 tacagtcaca attacagaaa cctgacgcag aatgtcttgc atgtttttac tcctttaaga
     4321 t
//
LOCUS       DATMXK010000138         2723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_93172, whole genome shotgun sequence.
ACCESSION   DATMXK010000138 DATMXK010000000
VERSION     DATMXK010000138.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93172"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VN363_02585"
     CDS             <1..495
                     /locus_tag="VN363_02585"
                     /inference="COORDINATES: protein motif:HMM:NF012263.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b N-terminal domain-containing
                     protein"
                     /protein_id="HWQ83422.1"
                     /translation="VYYVPSPDHAYDSIQYIMNGVAFGWLIRGIHHWGATLMVIFVFL
                     HLLRTFFTGSYKYPRELTWVTGVLLFLFTLGMGFTGYLLPWNQRAYWATTVGTEIPGT
                     APLIGPWITSLLRGGPDLSGVTLARFFSVHIWWLPAGIVLLIGIHLYLLIRLGISSMP
                     KKDD"
     gene            513..1703
                     /locus_tag="VN363_02590"
     CDS             513..1703
                     /locus_tag="VN363_02590"
                     /inference="COORDINATES: protein motif:HMM:NF012262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWQ83423.1"
                     /translation="MSDDYKQEYHEKYAREKEHGVKFFPDIIFKDMVFSFGVFVLLVG
                     LAIFVGVKNEPPADPSDSAYIPRPEWYFLFLFEMLKYFPGQIEWIGTTLIPAVAVIAL
                     LLLPFFDKNRARHWSKRKLGISIMSGVVLAMVVLSILAVITTPPQEEVALASTLGEQV
                     TIGQDVYSVQCVECHGAEGEGGEIIGVEGLEEVVLDPINSTDVMYTFTDDTLFNIVDY
                     GQPSLGMPPFGLAYGGELSRGDITALVNFMRYTWDDRAELPAEAAAASAIPELKPGEV
                     PSYEVHISVLSKRYCVSCHRPNKENNEYLMQSYEEILTSGDNAPNLIAGDLGSNLIRM
                     LHREEIEAGGPMPPTKALKAEYIDIWERWVMGGMPNTAADAAALSTTATEAVPEAPAP
                     SPTP"
     gene            complement(1810..>2723)
                     /locus_tag="VN363_02595"
     CDS             complement(1810..>2723)
                     /locus_tag="VN363_02595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558824.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="isocitrate/isopropylmalate family dehydrogenase"
                     /protein_id="HWQ83424.1"
                     /translation="YFKGVPSPVKRPEDVDMVVFRENTEDIYTGIEFELGTPENAQFL
                     TLLKDQFPKEYAKIRFPQTAGLGIKPVSREGTQRLVRAAIQWALENHRQSVTLVHKGN
                     IMKYTEGAFRNWGYELAEREFGERVYTWSQWERTKASQGEEAANLEQSAALKAGRLLI
                     KDVIADIVFQQTITRAREFDVLATTNLNGDYLSDALAAQVGGIGIAPGGNINYETGHA
                     VFEATHGTAPKYADKDVVNPGSVILSGEMMLRYMGWNEAADQIIAGMEKAITAKSVTY
                     DFHRLMEGATLLKTSEFGDAIIEQMNN"
BASE COUNT          634 a          736 c          678 g          675 t
ORIGIN      
        1 gtttattacg tgccttcccc agatcatgcg tacgacagca tccagtacat catgaacggg
       61 gttgcttttg gctggttaat ccgcggcatc catcattggg gggcaacatt gatggtgatc
      121 tttgtgtttt tacatcttct caggactttt ttcactggct cctataaata cccgcgcgag
      181 ctcacctggg tgacaggcgt cctcttattt ctattcactt tgggaatggg ctttacgggg
      241 tatctactgc cctggaatca gcgggcttac tgggcaacga ccgtgggcac agagatcccc
      301 ggaacggcac cattgattgg cccatggatc accagcctgc tccgaggtgg gccagacctg
      361 agcggtgtta ccctggcacg attcttctct gtccatatct ggtggttgcc ggcgggaatt
      421 gtactcctga tcggcatcca tctctatctg cttatccgcc tggggatcag ctctatgccc
      481 aagaaagatg attaaacacc gggagcacaa gtatgagtga cgattacaaa caagagtatc
      541 acgaaaaata tgcccgcgag aaagagcatg gggtgaaatt cttcccggac atcatcttca
      601 aggatatggt cttctctttt ggggtctttg tgctcctggt cgggttggca attttcgtcg
      661 gtgtgaaaaa tgaacctccg gctgatcctt ccgattcggc ttatataccc cgcccagagt
      721 ggtatttcct tttccttttt gaaatgctca aatattttcc aggccagatt gaatggattg
      781 gtacgacgct cattccagct gtggcagtga ttgcgctcct tttactgcca ttttttgaca
      841 agaatcgcgc ccgccactgg tctaagcgaa aattgggcat cagcatcatg agtggagtgg
      901 tgctggcgat ggtagtactg tcgatcttgg cagtgattac aacccctccc caggaagagg
      961 tcgctctggc ctccacattg ggagaacagg tcacaatcgg gcaggatgtc tattcagtcc
     1021 aatgcgttga atgccatggt gccgaaggag aaggtggaga gattatcggg gtggaagggc
     1081 tggaggaggt cgtcctggac ccgattaaca gtaccgatgt catgtataca ttcaccgatg
     1141 acaccctgtt caacatcgtt gattacggac agccatcttt agggatgccg ccgttcgggc
     1201 tggcctacgg tggggagcta agccggggtg atatcacagc cctggtaaat ttcatgcgtt
     1261 acacctggga cgatcgcgcc gagctgcccg cagaagcagc cgcagcctcc gccattcctg
     1321 agctcaagcc aggcgaagta ccatcatacg aagttcacat atccgtactc agcaaacgct
     1381 actgcgtttc ctgccaccgc cccaataaag agaacaacga gtacctgatg caaagttatg
     1441 aagagattct caccagcggt gataatgccc cgaatctcat cgccggcgat ctgggaagca
     1501 acctgatccg tatgctgcat cgtgaggaga tcgaagcagg tggaccaatg cctccaacca
     1561 aagcgctgaa agcagaatat atcgatatct gggaacgttg ggtgatggga ggaatgccaa
     1621 atactgccgc tgatgcagcc gcactcagca ctacggcaac ggaagctgta cctgaagcac
     1681 cggcgccatc cccgacaccc tgacagaata atagagatcc cgggcgaatt tacttttaat
     1741 cccacaagct aaccgggctg cctgatgact acaatcaggc agcccggtcg attctagcaa
     1801 ctttcaaaat cagttgttca tctgctcgat gatagcatcc ccaaattctg aggtcttcag
     1861 cagagttgcc ccctccatca aacggtgaaa gtcataggtc accgatttgg cagtgatggc
     1921 tttttccatt ccagcgataa tctgatcggc agcctcgttc cagcccatgt atcgcagcat
     1981 catttcccct gacagaatga ccgacccggg attaacgaca tccttatcag catatttcgg
     2041 agcagttcca tgagtagctt caaagaccgc gtgaccggtc tcgtaattaa tatttccacc
     2101 gggtgcaatt cctatcccac ctacctgggc agccagggca tcggatagat agtccccgtt
     2161 caggtttgta gttgccagaa catcaaattc tctggcccgg gtgatcgtct gctggaagac
     2221 aatatcagca atcacatctt ttatcaggag gcgacccgcc ttaagtgctg cagactgctc
     2281 caggttggct gcctcttcac cctggctggc tttagtccgc tcccactgcg accatgtgta
     2341 cacccgctcc ccaaactcac gttcggcaag ctcataaccc caattacgga aggcaccttc
     2401 cgtatatttc atgatgttcc ctttatgaac cagggtcacg ctctggcggt gattttccaa
     2461 agcccattga atggccgctc gtaccaggcg ctgggtgcct tccctggaga caggcttgat
     2521 ccccagcccc gccgtctgcg gaaaccggat cttggcatat tctttgggaa attggtcttt
     2581 taacagagtg agaaattgcg cgttttccgg cgtacccaat tcaaactcaa ttccggtgta
     2641 aatatcttcg gtgttctcgc ggaacacgac catatccaca tcttcaggac gtttgaccgg
     2701 ggacggcaca cctttaaaat acc
//
LOCUS       DATMXK010000139         3503 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_1194, whole genome shotgun sequence.
ACCESSION   DATMXK010000139 DATMXK010000000
VERSION     DATMXK010000139.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3503)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3503)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3503
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1194"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..851
                     /locus_tag="VN363_02600"
     CDS             <1..851
                     /locus_tag="VN363_02600"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWQ83425.1"
                     /translation="VPWISYNWGPYGVGANVLLAPGEAENGWAYTEDSLRECVRFAAQ
                     HGRKVAGLVLTCPDNPTGLTISPERQAALARAALEAGVGYVLFDWMYHYVTDAAPMEL
                     NAFLGLFSPAERLRLIFLDGITKSLGGSNIRCCHLIAPEPLVKFIVARASHGVIPSYY
                     SQAVAMAAYEMGYAEASRPIVEPTNQSRAALRTWLDLHGFTYILGQGYYAFIHVQPWL
                     EGRGWVDSEPLGQYLAEEYGVAIVPGAFFSPYGSSWLRFSYATPPERTLGALERLAEG
                     LYALEA"
     gene            855..>3503
                     /locus_tag="VN363_02605"
     CDS             855..>3503
                     /locus_tag="VN363_02605"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWQ83426.1"
                     /translation="MSEGFDPGLFAEKYHLALEQAERDNPQGGLCGFELEWNLLDSQL
                     RPLLTVGSGPEKQSFVDYLRAACLNPWTRDFSQLEVFHWMIEWATRPYYTPRAAVYEA
                     RLMEASLLNALDRAGREFGEQLYHWPGNLLYQTAIDHDSLPGAWGLAKRRYLERCVDL
                     YGESLATAGTHTNLSLPDPLLEWDFMHLSSIERGDSHLDEYKSEFYITATRLLRAFTA
                     LFIATSAATPFQARRQAGRPVVYLTPSDSVRNLTFPNPAGLDQPNLYRSYKDYLRISY
                     DLVRRGVRFGNNNWTPARARSFAEPVERLIHITSGKLSELYARGLYSLDEESPAGEMA
                     QQIEIQNLLARINLPMARVEVRTDDGGHSLAIDLANLTLKHLLLLRFYGDLDFGRAFR
                     YDREDIARARRSEEAAARDGLRAEIEHPMTGKPVAMRQFLKWTLDELRPLAEALSLWD
                     DLSPLQAMAEGAPNTAEKMRQETLEQIGVRLAGQEVEIPNELLIQMAEQRARQVMADV
                     ETIAGDLPAEPADHERLSEYLQHGREDAHANPQAPIRFRPGPEQIAAINYSDPASEIL
                     ALAQALIRIPSVTVGGEERLDEVRRAATLIYDTARNYGLEARYFDQARFPALLIAPPG
                     QMLAPVMLSGHFDVVAPDPDDSQFEPRISGDYLWGRGAADMKTVVATYLVWLKDSLHS
                     GRPYPPVNLLLIGNEENGETEATGTPHALRQLAGEHPEYAPRLVIAGERTGERGGELW
                     GEICTQNRGVLRFEVTATGKRAHSAASSGGSDLTERLLLARSDITGILSSHLTLTSPD
                     GWQSQARFPYIQVGIPGVFNITADRGVLGVEVRPIPQDRLQPLLLELQAYCASQALVL
                     EIPVCDDGIACDPENAPRLVIAGERTG"
BASE COUNT          659 a         1070 c         1118 g          656 t
ORIGIN      
        1 gtgtgccgtg gatctcgtat aactggggac cctacggggt aggggcgaat gtgctgctgg
       61 cgccgggcga ggcggagaat ggctgggcat ataccgaaga cagcctgcgc gagtgcgtgc
      121 gcttcgccgc ccagcatggg agaaaggtgg ccgggttggt gctgacctgc ccggataacc
      181 cgaccggcct gaccatcagc ccggagcggc aggccgccct ggcgcgggct gccctggagg
      241 ctggcgttgg ctatgtactc ttcgactgga tgtaccacta tgtgaccgat gccgcgccaa
      301 tggaattgaa tgccttcctc gggttgttca gccccgcaga acgcctgcgc ttgatcttcc
      361 tggacggcat caccaagagc ctgggtggct caaatatccg ctgctgccac ttaatcgcgc
      421 cagaacccct ggtgaaattt attgtagccc gtgcttcaca tggggtgatc ccctcttatt
      481 attcacaggc cgtggcgatg gcagcctatg agatgggtta tgccgaagcc agccggccaa
      541 ttgtcgaacc gaccaaccag agccgggcgg cgctgcgcac ctggctggat ctgcatggtt
      601 ttacgtacat cctgggccag ggttactatg catttatcca tgtgcagccc tggctggagg
      661 ggcgcggctg ggtggacagc gagcccctgg gacaatatct ggcagaagag tacggtgtgg
      721 caatcgtgcc gggggcgttc ttttcgccgt atggcagcag ctggctgcgc ttctcttacg
      781 ccacaccccc cgagcgcacc ctgggagcgc tggaacggct ggccgaagga ctgtatgcgc
      841 tggaggctta gccaatgagc gaaggtttcg acccgggctt attcgccgaa aaatatcacc
      901 tggcactgga gcaggccgag cgggacaacc cgcagggcgg gctgtgcggc tttgagctgg
      961 agtggaattt gctcgacagc cagctccggc cgctgctgac tgtcggctcg ggaccggaga
     1021 aacaatcctt tgtagattat ctgcgggcag cctgccttaa cccctggacg cgcgacttca
     1081 gccagctgga agtattccac tggatgattg agtgggccac ccgcccgtat tacaccccgc
     1141 gcgccgcggt gtacgaagcg cgcctgatgg aagccagcct gctcaacgcc ctggaccgcg
     1201 ccgggcgcga gtttggcgag cagctgtacc actggcccgg gaacctgctc taccagacgg
     1261 ccatcgacca cgattcgctg ccaggcgcct gggggctggc gaagcgccgc tatctggagc
     1321 gctgtgtcga tctgtatggc gaatcgctgg caactgctgg aacgcacacc aacctgtctt
     1381 tacccgaccc gctgctggag tgggatttca tgcatctcag ctccatcgag cgcggcgaca
     1441 gccacctgga cgagtacaaa agcgaattct atatcaccgc cacccgcctg ctgcgggcat
     1501 ttacggcttt gtttatcgcc accagcgccg ccaccccttt ccaggcgcgc cgccaggctg
     1561 gccggccggt tgtctacctg accccatccg attctgtccg caacctcacc ttccccaacc
     1621 cggccgggct ggaccagccc aacctgtacc gttcctataa ggattatctg cggatctcct
     1681 acgatctggt ccgccgggga gtgcgctttg gcaacaacaa ctggacaccc gcccgggcgc
     1741 gctcatttgc tgaaccggtc gagcggctga tccatatcac ttccggtaag ctcagcgagc
     1801 tgtacgcccg gggactgtat tcgctcgatg aggaaagccc ggccggtgag atggcgcagc
     1861 agatcgaaat ccagaacctg ctggcgcgca tcaatctgcc catggcccgg gtggaagttc
     1921 gcaccgacga tggtgggcat tcactggcta tcgacctggc aaacctgacc ctgaaacacc
     1981 tgcttttgct gcgtttctac ggtgacctgg attttggacg cgcattccgc tacgatcgcg
     2041 aggatatcgc ccgcgcccgg cgcagcgaag aagcggctgc ccgggatggc ctgcgagctg
     2101 agatcgagca ccccatgact ggtaagccgg tggcgatgcg gcagttcttg aagtggaccc
     2161 tggacgagct gcgaccgctg gcggaagccc tgagcctgtg ggatgatctc agtccgctgc
     2221 aggcgatggc agaaggcgct ccgaatacag ctgaaaaaat gcgccaggag acattggagc
     2281 aaatcggagt ccgcctggcc ggccaggagg tcgaaattcc caatgagctg ctgatccaga
     2341 tggcagagca gcgcgcccgc caggtgatgg cggatgtcga aaccatcgcc ggggatctac
     2401 cggcagagcc ggcggatcat gagcggttga gcgaatacct gcagcacggg cgggaggatg
     2461 ctcacgccaa ccctcaggct ccgatccgct ttcgtcccgg accggaacag atcgcagcga
     2521 ttaattactc cgatccggcg agcgagatcc tggccctggc gcaagcgctg atccgcatcc
     2581 catccgttac ggtgggtggg gaggagcgcc tggatgaagt gcgccgggcc gcgaccctga
     2641 tttacgacac tgcgcgcaac tacgggctgg aagcgcgcta tttcgatcag gctcgttttc
     2701 cggctttatt gatcgcccca ccggggcaaa tgctggcgcc tgtgatgttg agcgggcatt
     2761 tcgatgtcgt tgccccagat cccgacgaca gccagttcga gccgcgcatc tccggggatt
     2821 acctgtgggg gcgcggggct gcggatatga aaactgtggt ggcgacctat ctggtatggc
     2881 tgaaagacag cctgcattca ggaaggcctt acccgccggt gaatttgctg ttaattggga
     2941 atgaggagaa cggcgagacc gaagccaccg gaaccccaca tgccttacgc cagctggcag
     3001 gcgagcaccc ggagtatgcc ccacgcctgg tgatcgccgg agaacgcacc ggcgagcgcg
     3061 gcggtgagct gtggggggag atctgcaccc agaatcgggg ggtgctgcgc tttgaagtaa
     3121 ccgccaccgg gaagcgcgcc cacagcgcag ccagctcagg cggcagcgac ctgaccgagc
     3181 gtttgctgct ggcgcgcagc gatatcaccg gaatattatc cagccatctg accctgacca
     3241 gccccgacgg ctggcagtct caagcgcgct tcccttatat tcaagtgggc ataccggggg
     3301 tgtttaatat cactgctgac cggggtgtgc tcggtgttga ggtccgcccc attccgcaag
     3361 acaggctgca gcccttactg ctggagctgc aggcgtactg cgccagccag gcgctggtgc
     3421 tggaaatccc ggtctgtgac gatgggattg cctgtgaccc ggagaatgcc ccacgcctgg
     3481 tgatcgcagg agaacgcacc ggc
//
LOCUS       DATMXK010000140         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_128172, whole genome shotgun sequence.
ACCESSION   DATMXK010000140 DATMXK010000000
VERSION     DATMXK010000140.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128172"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..704)
                     /locus_tag="VN363_02610"
     CDS             complement(<1..704)
                     /locus_tag="VN363_02610"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWQ83427.1"
                     /translation="MKIIIPGGSGMIGTLATTMFARQGHTVIILSRTPEASLNRLVQQ
                     AGYLANVVVRGWDTATAEGWGELVDSDTAIINLVGVNLGGKRWTPAQKKLILESRTKA
                     GQAIVQAAEQARAKPAVVLQVSGSGYYGARQDELISESSPAGSDFPAEVCQAWETSTA
                     ALEALGVRRVVMRTGVVLSERGGALPRLLLPFRLMIGGPLGNGRQWLAWIHELDQIAA
                     FQYFIENRSCHGVYNVT"
     gene            complement(796..1653)
                     /locus_tag="VN363_02615"
     CDS             complement(796..1653)
                     /locus_tag="VN363_02615"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="metallophosphoesterase"
                     /protein_id="HWQ83428.1"
                     /translation="MNEPERTMSRREFLKISAMALAGGLAGAGALDYARRVEPEWIET
                     TQRTLHLPRLDPAFNGLRLVQISDIHMDGWMDGNRFSAIVSEVNALQPDLIAITGDLV
                     TLSHHDHYAELARSLGELRAAYGVSVVLGNHDYYSGVHKVRQVLKTAGVRELSNQVWT
                     IHKGGSSLHIAGLDCAFFGHDRLGQVLEELPEGGAAILLAHEPDTADRTAASGRFDLQ
                     LSGHSHGGQVVLPIFGAPILPRLARIYPFGYYQIKGMHLLVNRGLGMVHIPVRFNARP
                     EISVYEFTA"
     gene            complement(1646..2041)
                     /locus_tag="VN363_02620"
     CDS             complement(1646..2041)
                     /locus_tag="VN363_02620"
                     /inference="COORDINATES: protein motif:HMM:TIGR03618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PPOX class F420-dependent oxidoreductase"
                     /protein_id="HWQ83429.1"
                     /translation="MTALQISEKYYAWLKSRAFAHLATLMPDGSPQVTPVWIDTDGEM
                     ILVNTARGRQKDLNMQRDGRVALSIMNPDNPYDYVQVRGLVVLSTENGAREHIDELSQ
                     RYLGHAYQANDPDQVRVIYQIRPLNLLDE"
     gene            complement(2093..>2541)
                     /locus_tag="VN363_02625"
     CDS             complement(2093..>2541)
                     /locus_tag="VN363_02625"
                     /inference="COORDINATES: protein motif:HMM:NF015553.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="cadmium resistance transporter"
                     /protein_id="HWQ83430.1"
                     /translation="PEEWMIGLLGLIPLGLGLKVLFSKEEEEGEDEAEEQKEQVMESS
                     HKYGSLVASITFLTIASGGDNIGVYIPLFSSQTWNEIGLILSAYALLTAVLLYISYRF
                     ANLKYVGETIEKYERIIVPVVFIGLGIMIMSENGVFSFLASIFGLS"
BASE COUNT          522 a          793 c          680 g          546 t
ORIGIN      
        1 gtgacattgt ataccccgtg gcaggagcgg ttttcaatga aatattggaa tgcagctatt
       61 tggtccagtt cgtggatcca tgccagccac tgcctgccat tccccagcgg cccgccgatc
      121 atgagcctga acggcagcag gaggcgcggc agggcgccgc cgcgctccga aagcaccacc
      181 ccggtgcgca tgaccacccg gcgcacaccc agggcttcca acgcagcagt ggaggtttcc
      241 caggcctggc atacttccgc cgggaagtcg ctgcctgccg ggctgctctc actgatgagc
      301 tcatcctgcc gggcgccata ataaccggac cctgagacct gcagcaccac agccggcttt
      361 gcgcgggctt gctccgcagc ctgtacaatc gcctgcccgg ccttcgtccg gctttccagg
      421 atgagcttct tttgggccgg ggtccagcgc ttgcctccca ggttcactcc caccaggttg
      481 attatagctg tatcggagtc gactagctcg ccccaaccct cggcggttgc ggtatcccag
      541 ccgcgcacca ccacattggc caggtaacca gcttgctgga ccaaacggtt caggctggct
      601 tcaggtgtcc ggctgagaat aatcacggtg tgcccctgcc tggcgaacat cgttgtggcc
      661 agggtgccta tcatccctga cccaccagga attattattt tcacagaact gcctccaaat
      721 gttcgtccgt aggcaagctt tagcttgact cctgtccggc tgaagccgaa cctacgtgtc
      781 cggctaaagc cgaacctaag cagtgaattc atacacactg atctccgggc gggcgttgaa
      841 acgcaccgga atatgcacca tccccaggcc gcggttcacc agaaggtgca tccctttgat
      901 ttgataatat ccgaaggggt agatgcgcgc caaacggggt aagatcggcg cgccaaaaat
      961 tggcagcacc acctgcccgc cgtgcgaatg ccctgaaagc tgcaggtcaa agcgcccgct
     1021 ggcagctgtc ctgtcggcag tatctggctc gtgggccagc aggatggccg ctccaccctc
     1081 cgggagctcc tccagcacct gccccaggcg gtcgtgtcca aaaaaggcgc agtccagccc
     1141 ggcaatatgc aggctggagc cccctttatg gatggtccat acctggtttg aaagctcgcg
     1201 cactccggca gttttcagta cctggcggac tttgtgaacc cctgaataat aatcatggtt
     1261 gcccaggact acgctgaccc cgtaagcagc gcgcaattcg cccagcgacc gggccagctc
     1321 ggcataatgg tcgtgatggg aaagggtcac caggtcgccg gtgatcgcga tcaggtcagg
     1381 ctgcagggca ttcacctcgc tcacaatggc gctgaagcga ttgccatcca tccagccatc
     1441 catatggata tcgctgatct gcaccaggcg cagcccatta aaagccgggt ccaggcgcgg
     1501 cagatgcagc gtccgctggg tggtctcgat ccattccggc tctacccgcc gggcataatc
     1561 cagggcacct gcgcctgcca gcccgcccgc cagcgccata gcgctgattt ttaagaattc
     1621 ccgcctgctc attgtccgtt ctggctcatt catcgagcag attcaatggg cggatttggt
     1681 aaataacccg cacctggtcc gggtcgttag cctggtaggc gtgccccaga tagcgctgcg
     1741 aaagctcgtc gatatgctcg cgggctccgt tctcggtgct caacacaacc aggccgcgca
     1801 cctggacata atcgtatgga ttatcggggt tcataatcga gagcgccacg cggccatccc
     1861 gctgcatatt gaggtccttc tgccggccgc gagctgtatt caccaggatc atttccccgt
     1921 ctgtgtcgat ccagaccggg gttacctgcg ggctgccatc cggcatcaat gtcgccaggt
     1981 gggcaaaagc ccggctctta agccaggcgt aatatttttc agaaatttgt agtgctgtca
     2041 tgaatactcc ttaccaggtt attggttacg acaattttac tcggatttaa gctcaggata
     2101 acccgaatat actcgccaga aagctgaaga ccccgttttc gctcatgatc atgatcccca
     2161 ggccgatgaa tacgaccggc acaataatcc gctcgtattt ttcgatggtc tcgcccacgt
     2221 acttgaggtt tgcaaagcga tagctgatat agagcagaac ggcggtcagc aaagcatatg
     2281 cgctcaagat cagcccaatc tcattccagg tctgcgagga gaaaagcggg atatacacgc
     2341 cgatattgtc ccctccgctg gcgatggtca gaaaggtaat gctcgccacc aggctgccgt
     2401 atttatggga ggattccatc acttgttctt tttgctcttc cgcttcatcc tcaccttcct
     2461 cttcttcctt gctgaagagc actttcaggc ccaatcccag ggggatcagt cccagcagcc
     2521 cgatcatcca ttcctcggga a
//
LOCUS       DATMXK010000141         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161628, whole genome shotgun sequence.
ACCESSION   DATMXK010000141 DATMXK010000000
VERSION     DATMXK010000141.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161628"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(55..>2245)
                     /locus_tag="VN363_02630"
     CDS             complement(55..>2245)
                     /locus_tag="VN363_02630"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83431.1"
                     /translation="LLSILLAACSTPAPTVVATEAPTAVPCPTCPPVPECPTCPEPVV
                     QDVPYQDDWANSPHADITSEAFIHWDEEDPAEVPTSCAKCHSTPGYLDYIGADGSAVG
                     AVDVASPIGTTVECIACHNPTTATKTSVVFPSGVEVTGLGAEARCMECHQGRSSSTQV
                     NASLEENGVAEDLDTPNAELSFINIHYFAAAASLYGTQTKGGYEYDGKAYDFKNDHVE
                     GYDTCIGCHNSHTLELKVEACATCHTGVASVEDLRAIRMQGSQADYDGDGDVTEGIAA
                     EIEGLQALLIQSIQAYAAEKSGAAVAYDVAAHPYFFLDLNTNGTVDDDERNSDNAFNA
                     WTGRLLKAGYNYQTSVKDPGSYAHGGKYIIQLLYDSVEDLNSVLDAPVEFSTARRLDP
                     GHFAGSEEAFRHWDAEGEVPADCAKCHSADGLPLFLKEGVNISTHVVNGLNCTTCHAS
                     LADFSLYPVAEVTVPSGKRISFGEADNSNICLNCHQGRESVVSVNAAITRSGATGDDQ
                     VTEGLNVRNPHYFAAGSTLFGSEASGAYEYQGKQYNGRNLHANGFSTCINCHNAHALE
                     NNVVACAGCHTGVSTAEDLENIRMGGEAPVDFDGDGDVAEGVANEVATLEEALYAAIQ
                     QYAADRAGSPIVYNAAAYPYFFVDTNGDGVADPDESVRDNGFASWTPRLLKAVYNYLW
                     IAKDPGAFAHNADYSMQVLYDSLQDIGGGGAVSGMTRPPVAVPAPAP"
BASE COUNT          491 a          586 c          636 g          532 t
ORIGIN      
        1 atcaggggct gtctggcata agctggacag cccctgatga ttcagagtgg atatttaggg
       61 tgctggtgca ggtactgcta ctggtgggcg agtcatcccg gaaactgccc cgccgccgcc
      121 gatatcttgc aagctatcat agagaacttg catggagtaa tcggcattgt gggcaaaggc
      181 tccaggatcc ttggcgatcc acagatagtt atagactgcc ttgagcaggc gtggtgtcca
      241 gctcgcaaag ccgttgtcac gcacggattc gtcaggatca gccacaccat cgccgttcgt
      301 atcgacaaag aagtatggat aggctgcggc gttatagacg atcggtgacc cggcccggtc
      361 agctgcgtat tgctggatgg ctgcatagag agcttcttcc agagtagcaa cctcgttagc
      421 cacaccttcg gcaacgtcgc cgtcgccatc gaagtcaacc ggagcctcac cacccatgcg
      481 gatgttttcc aggtcttctg cggtgctgac gcctgtgtgg cagccagcgc aggcgactac
      541 attgttttcc aaggcatggg cattatggca gttgatacag gtagagaagc cattggcgtg
      601 tagattgcga ccattatatt gcttgccctg atattcatag gcgccgctgg cttcactacc
      661 aaatagcgtg ctgccggctg caaaataatg gggattgcgt acgttgagac cttccgttac
      721 ctgatcgtcg ccggttgcac cgctgcgggt aatggcagca ttgacactga ctaccgattc
      781 acgaccctga tggcagttca gacaaatgtt ggaattgtct gcttcgccaa agctgattcg
      841 cttgccgctt ggtacagtga cttctgcgac cggataaagg ctgaaatcag ccagactggc
      901 atggcaggtg gtgcaattca ggccattcac cacatgggtg gatatattta cgccttcctt
      961 caggaacagg ggtaaaccat ccgcagagtg gcattttgca cagtctgctg gaacttcgcc
     1021 ttctgcatcc cagtggcgga aagcttcttc agagccagca aagtgaccag gatcgagccg
     1081 gcgcgcagtg gaaaattcaa ctggagcatc caacacgctg ttgagatcct caactgaatc
     1141 gtacagcagc tgaataatgt attttccacc gtgggcatac gaaccgggat ccttgactga
     1201 ggtctggtag ttgtaaccag ctttcagcag gcgtccggtc caggcattga aagcattatc
     1261 actgtttcgc tcgtcgtcat ccacagtgcc attcgtgttc aagtccagga aaaagtaagg
     1321 gtgggcggca acatcatagg cgaccgctgc tccagatttc tccgcagcgt atgcttgaat
     1381 ggactggatg agtaaagctt gcagaccttc gatctctgct gcaatgcctt cagttacgtc
     1441 gccatccccg tcataatctg cctgagaacc ctgcatgcgg attgcacgca gatcttcaac
     1501 gctggcaacg ccagtatggc aggtggcgca ggcttcaacc ttgagctcta aggtgtgtga
     1561 attatggcag ccgatgcagg tgtcatagcc ttcgacgtgg tcgtttttga agtcgtaggc
     1621 tttgccatcg tactcgtaac cgcctttggt ctgggtgcca tagagtgacg cggctgcagc
     1681 aaagtagtga atgttgataa aactcagctc ggcattcggg gtatccagat cttccgccac
     1741 accgttctct tccagcgagg cgttcacctg ggtggaggaa gaacgaccct gatggcattc
     1801 catgcagcgt gcttcagcgc ctaacccggt tacctcaacc cccgatggga agacgacact
     1861 ggttttggta gctgttgtag gattgtggca ggcaatacat tcaacagtgg taccaattgg
     1921 gctggcaaca tcgactgctc caaccgcact tccatccgcg ccgatgtagt ccagatagcc
     1981 cggcgtgctg tggcatttag cgcagcttgt cggcacttcg gctggatctt cttcatccca
     2041 atggatgaaa gcttcggaag taatatcggc atggggagag ttggcccaat cgtcctggta
     2101 gggcacatcc tggacgactg gttctggaca ggttgggcat tctggcacgg gcgggcaggt
     2161 tgggcacgga accgcagttg gggcttcagt agccactacg gttggtgccg gtgtgctgca
     2221 cgctgccagc agaatgctca ataag
//
LOCUS       DATMXK010000142         2488 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_15172, whole genome shotgun sequence.
ACCESSION   DATMXK010000142 DATMXK010000000
VERSION     DATMXK010000142.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2488)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2488)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2488
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15172"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1500)
                     /gene="acnA"
                     /locus_tag="VN363_02635"
     CDS             complement(<1..1500)
                     /gene="acnA"
                     /locus_tag="VN363_02635"
                     /EC_number="4.2.1.3"
                     /EC_number="4.2.1.99"
                     /inference="COORDINATES: protein motif:HMM:NF006757.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aconitate hydratase AcnA"
                     /protein_id="HWQ83432.1"
                     /translation="MGKDAFNSRQKLYIRGSELVYYRLAKLEEDGLTRLDRLPFSIRI
                     MLEGLLRQCNGIEITQQDVLNLAAWQPQAEHRPSLPYRPARVIMQDFTGVPAVVDLAA
                     MRDAVKRLGGDPKRINPLVPVDLVIDHSVQVDFFASPDALTRNAELEFQRNRERYEFL
                     HWGQQAFKNFRVVPPATGIVHQVNLEYLANVVLATEEEGVLVAYPDTLVGTDSHTTMI
                     NGLGVVGWGVGGIEAEAAMLGQPIDMVTPEVIGFKLSGELKEGVTATDLTLTVTQILR
                     QKGVVGKFVEFYGPGLAAMSLPDRAAIANMAPEYGATMGFFPVDDETLRYLRLTGRSD
                     EVVERVEVYCKAQGLFRTAETPDPVFTDTLELDLGSVQPSLAGPKRPQDRVTLAEMQT
                     AFQAALVAPKAERGYELKPEERARTATVYGEGASTSQNMIDMGHGAVVIAAITSCTNT
                     SNPSVMLGAGLVAKKAIERGLKVQPYVKTSLAPGSRVVTEYLQKAGLVAP"
     gene            complement(1576..2481)
                     /gene="folP"
                     /locus_tag="VN363_02640"
     CDS             complement(1576..2481)
                     /gene="folP"
                     /locus_tag="VN363_02640"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR01496.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004156 - dihydropteroate synthase
                     activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWQ83433.1"
                     /translation="MAAFSPLTIAHKTLEWGARTYVMGILNITPDSFSGDGLIPASPQ
                     SQQMVVDRAVEQAQRFVSAGADILDIGGESTRPGASPITVQEELDRVIPVIQAISNQM
                     DILISVDTYHAVVAEAALEAGANIVNDVWSLQVDPDMAGVVARYSAPVVLMHNHSSWA
                     HTEVREKLGGRYVGKPFVDMILDIQNELKQSLAIAHAAGIQDENIILDPGVGFGMTVS
                     ENLELIDRLAEIRSLGYPVLLGPSRKSFIGYTLDLPPDQRLEGTAAAVAVGIARGVDI
                     VRVHDVEFMVRVARMTDAIVRRTGK"
BASE COUNT          566 a          745 c          632 g          545 t
ORIGIN      
        1 gggggcgacc agcccggctt tttgcaggta ttcggtgaca acccgcgaac ccggtgccaa
       61 gctcgtttta acatacggct ggactttaag acctcgctca atcgcttttt tggcaaccag
      121 tcctgctccc agcatgaccg agggatttga cgtattggtg caggatgtga tcgcagcaat
      181 gacaactgca ccgtgcccca tatcgatcat attctgggat gtcgatgccc cttcaccata
      241 gaccgtcgct gtacgagctc tctcttcggg cttgagctcg tagccgcgct ctgctttcgg
      301 cgcgaccagg gccgcctgaa atgcagtttg catctccgcc agcgttaccc ggtcttgagg
      361 tcgcttgggt cctgccagac tcggctggac actgcccagg tcaagctcca gggtatccgt
      421 aaacaccgga tcgggagtct cagcagtccg gaaaagcccc tgggctttac aatagacctc
      481 cactcgttca acgacttcat ccgagcgccc ggtcaggcgg agataacgta aagtttcgtc
      541 atctaccggg aaaaacccca tcgtcgctcc atactccggt gccatattgg caatcgccgc
      601 ccggtcgggc agcgacatcg cggccagacc cgggccataa aactcaacaa atttgcccac
      661 gacacctttc tggcgcagaa tttgagtgac cgtcagggtc agatctgttg ccgtaacgcc
      721 ttcttttagc tcaccagaca acttaaagcc gatgacctca ggtgtgacca tatcgatcgg
      781 ctgacctaac atagccgcct ctgcctcgat accgcccaca ccccagccga ccacgcccag
      841 gccattaatc atggtggtat gcgaatcggt cccgaccagc gtatccgggt atgcaacaag
      901 aacaccttct tcttcagttg ccagaacaac atttgccagg tattccaggt tcacctgatg
      961 gacaatgccg gtggcaggtg ggacaaccct gaaattctta aacgcctgct gtccccaatg
     1021 taagaactcg taacgctcac gattgcgttg gaactccagt tcggcattgc gcgtcagcgc
     1081 atcgggtgag gcaaaaaaat ccacctgaac ggaatggtcg attaccagat cgaccggcac
     1141 cagggggttg atccgtttgg ggtcgccgcc caggcgtttt acagcatctc gcatggctgc
     1201 caggtcgacg acagccggga cgcccgtaaa atcttgcata atgacccggg ccgggcgata
     1261 tggcagcgat gggcggtgct cggcctgggg ctgccaggct gccagattta gtacatcctg
     1321 ctgggtgatc tcaataccat tgcactggcg gagcaaacct tccagcatga tccggattga
     1381 aaatggcagc cggtcgagcc gggtcagccc atcttcttcg agctttgcta accgataata
     1441 aaccagctcg ctgccccgga tatacaattt ctggcgggaa ttaaaggcat ccttccccat
     1501 gtgtttctcc ttatattgac tgataactga gtgcggatct atcagaatct gcgattaatt
     1561 tacctatatt gcgagctatt tcccggttcg gcgaacgatc gcatccgtca tccgggctac
     1621 ccggaccata aactccacat cgtgaacgcg tacaatatca acccctcggg cgatccctac
     1681 agctacagca gcagctgtgc cttccaggcg ctggtctgga ggcagatcga gggtatagcc
     1741 gataaacgac ttccgggaag gccccagcaa gacgggatag cccaggctgc ggatttccgc
     1801 caggcggtcg atcaactcca ggttttcgct aactgtcatc ccaaacccca cgcctggatc
     1861 aaggataatg ttctcatcct ggatcccggc ggcatgcgca atcgccaaac tttgcttaag
     1921 ctcgttctgg atatccagga tcatatccac gaacggcttg cctacatagc gcccacccaa
     1981 tttttcccgc acctcggtat gtgcccatga actatgatta tgcatcagga cgaccggagc
     2041 ggaatagcgt gcaaccactc ccgccatatc gggatcgacc tgcaggctcc acacatcgtt
     2101 gacgatattt gccccggctt ccagagccgc ttcagcgaca acggcatggt atgtatccac
     2161 ggatatcagt atatccatct ggttggagat tgcctggatg actggaatta cccgatccag
     2221 ctcttcctgg acagtgatcg gagaagctcc cggacgagtg ctttcgccac cgatatcgag
     2281 aatatctgcg ccagcactaa caaaacgctg agcctgctcc acggcgcgat caacgaccat
     2341 ctgctggctc tgcggcgagg cagggatcaa accatcccct gaaaaactat ccggggtaat
     2401 attcaggatg cccatgacat aagtgcgagc gccccattcc agagttttat gggcaattgt
     2461 gagcggagag aaagctgcca tttatacc
//
LOCUS       DATMXK010000143         3669 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_125129, whole genome shotgun sequence.
ACCESSION   DATMXK010000143 DATMXK010000000
VERSION     DATMXK010000143.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3669)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3669)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3669
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125129"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..616)
                     /locus_tag="VN363_02645"
     CDS             complement(<1..616)
                     /locus_tag="VN363_02645"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HWQ83434.1"
                     /translation="MIEIANSISRRYQLQAQIVAKRLPQVLQRRGLEAPFAEFLVTST
                     QGMVLLFAILDLPQVRRLEAYTTPELLHHLSTDLQGLPVFLSNSNGLRYAIPLSPLPR
                     LPKLIQFPGIERGWVLLGVNHAGESVNQPWSKLGHLLVAGKTGSGKSVFLRSLVYQAL
                     VEGAQLLLVDLDGATFPMLANHPVMLAPISTTAQAAHDAVERALG"
     gene            complement(609..1607)
                     /locus_tag="VN363_02650"
     CDS             complement(609..1607)
                     /locus_tag="VN363_02650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83435.1"
                     /translation="MKNHKLLLLFPLLLFGWILTACDDAAYSSADPNVSGVQARATLE
                     FVQAVQTSTAQALYAAEWQATVQAASRQDAIQAAYVAAQATDESIRSAAAATRTQQVW
                     NATTTADSSQATATAAANATAVSWSSTATQSAWNNQATAEVASVQALQTAQAAQADMA
                     RMAAERQRTINKIQAAAPWVILAIAVPLVAYLAWVWGNTEALRRRAIPRDPRGDAPIL
                     ILDDHGRRNVIDPDLFFGAAVSIGKGGQPEAPQLAAPELQSQVKARDQAVDLVSRGLP
                     GQPQRRRALPTLTQIPSNPLLGNVRVVEPEEVKDWISDVLPRIQRQAFQEGEIEDD"
     gene            complement(1631..2107)
                     /locus_tag="VN363_02655"
     CDS             complement(1631..2107)
                     /locus_tag="VN363_02655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83436.1"
                     /translation="MKKIGFFIALVLSVLLVVAACGPSDNAVEIAQAQNVIEAARAAQ
                     DASRAAQIASQGLSDMGRGQTLILVLMTLMVVLLIGMAAYIVIQRLIWKHSQVQPRNT
                     GRWMSGPNAHWKRVEGSDPYQQLLLQQQMLLTQFLQQSQVESEEDDRPQLPTEWWG"
     gene            complement(2135..>3669)
                     /locus_tag="VN363_02660"
     CDS             complement(2135..>3669)
                     /locus_tag="VN363_02660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83437.1"
                     /translation="KPETWKRLRWLVKQVDSEPRWSRGKDGRPHQSSRYYRVTMNPPL
                     TPFDEQSLRSWLYRQLAQGKTPVAVIQSALETPVDELIPWPDQVASEEAIDEPHSVQD
                     VLQAVCGSIPESQKAEFREFADKLAHHLMPPKDLVFLTHYFVSHWLPRLGPGPGWFVT
                     LMRDRGYINQRTGEVRDEIHLREGYAEAARWLGLKRAKTVWEWLRTGEVATFVRETGR
                     EIGSWEDAPRRFKVCLGEPMTESDQARANESLSAVAIGASDIHNPQNGNDLIGANDTH
                     SSATDPNLVGASDTHREDQSADLIGAIDIHNGAPDIHKPGASDIHDGAVDTHKNGASD
                     TPDWRDWHSLNTLALGLNHKKNTPTTTDADIDPDLEAESDRLKKGVVGMEWNLSDLLV
                     RNRVSTKNHELLLEKGLTAQAFVSWLLYAASTGGNGIHDPIAHTVSRLIPNPSRGAGS
                     AFDELAELPANDLADMLTRVLAGQNPWNQIWRKTMDGAPRSRLRMLADQLGIPVSDLG
                     NW"
BASE COUNT          803 a         1044 c          969 g          853 t
ORIGIN      
        1 ctcccagagc gcgttcaacc gcatcatgag ctgcctgggc tgtggttgat attggggcta
       61 acattactgg atgattagcc agcatcggga aagtggcgcc gtccaaatcc accaatagaa
      121 gctgagcacc ttcaacaagc gcctggtaga ctagagatcg taagaacacc gatttacctg
      181 agcccgtctt acccgccact agtaaatggc caagtttcga ccagggttgg tttactgact
      241 cgccggcatg gttgacgccc agcagcaccc atccacgctc gattcctgga aattggatca
      301 gctttggtaa ccgtggtagt ggtgagagcg ggatggcata acgaagccca ttcgaattag
      361 agaggaacac cggcagcccc tgtagatcgg tgctcaggtg atgcaacagt tcaggggtag
      421 tgtaggcttc cagacgccgt acctgtggta aatccaggat ggcaaacagc agcaccatcc
      481 cctgcgtgga tgtaaccaaa aattctgcaa aaggtgcttc caggccacgc cgctgcaaca
      541 cttgcggtag ccgcttggca acgatctgcg cctggagctg atagcgtcgc gatatagagt
      601 tcgcaatttc aatcatcttc gatctctcct tcctggaacg cttgccgttg aatgcgcggc
      661 aacacatcag atatccagtc tttcacttct tcaggctcca ccacccgtac attgcctaat
      721 aaagggtttg acggaatttg cgtgagggta ggtaaagcgc gccgtcgttg gggttggccg
      781 ggtaggcctc ggctgacgag atcgacagcc tggtcgcgcg ccttcacctg tgattgcagc
      841 tcaggagctg ccaactgcgg agcttccggt tggcctccct taccgatcga caccgcggca
      901 ccgaagaata aatctgggtc aatgacatta cgccggccgt ggtcatccag gatcaggatt
      961 ggcgcatccc cacgcggatc acgcgggatg gcgcgccgac gcagggcctc ggtgttaccc
     1021 cagacccagg ccagataagc caccaaaggg actgcgattg ccaggatcac ccagggagca
     1081 gccgcttgaa ttttgttgat cgtacgctgc cgttcagctg ccatccgggc catatcggcc
     1141 tgggctgcct gagcggtctg cagcgcctgc acgctggcga cctcggctgt ggcctggttg
     1201 ttccaggccg attgggtagc agtgctggac cagctcaccg ccgtggcatt agcggcggca
     1261 gtagccgtcg cctggctgct atcagctgta gtagtggcat tccatacctg ttgcgttcgg
     1321 gtggctgctg ccgcagagcg aatgctttca tcggtcgcct gggcagccac atatgcagcc
     1381 tgaattgcat cctgcctcga agctgcctgg acagtcgcct gccattcggc agcataaagt
     1441 gcctgcgcgg tagaggtctg cacggcttgc acaaactcca atgtcgcccg agcctgcaca
     1501 ccggagacgt tcgggtctgc actgctatat gcggcatcat cacaagcagt caggatccat
     1561 ccaaacagaa gcagagggaa cagtaagagc aacttgtggt ttttcatctg caccttcctc
     1621 ccttctgcat ctatccccac cactcggtag gtagctgagg tcgatcatcc tcttcggatt
     1681 cgacctggct ctgttgcaga aactgggtaa gcagcatctg ctgttggagg agcaattgtt
     1741 ggtatgggtc agaaccttcc acccgcttcc agtgagcgtt tggcccactc atccagcggc
     1801 ctgtgttcct tggctgaacc tggctgtgct tccaaatcag gcgttggatg acaatatagg
     1861 cagccatccc aatcagcaaa accaccatca gggtcatgag caccaggatc aacgtttgac
     1921 ctcgccccat atcgctcagg ccttgggagg cgatttgcgc cgcccgggaa gcatcctgag
     1981 cggcgcgggc tgcctcgatc acattctgag cttgagcgat ctccactgca ttgtcgcttg
     2041 gcccgcatgc ggctacaacc agtaaaaccg acaaaaccaa agctataaag aaaccgatct
     2101 ttttcatgtc acacctccat gtaactgaac caaatcacca attaccaagg tccgaaaccg
     2161 gtatacccaa ttgatcagcc agcattcgca gacgtgaacg tggcgcgcca tccattgttt
     2221 ttcgccagat ctgattccag gggttttgtc cagccaacac cctagtcagc atgtctgcca
     2281 gatcgttggc tggcaattca gcaagctcgt caaaggcact ccctgcgcca cggcttggat
     2341 tcgggatcaa gcgactgacg gtatgcgcga taggatcatg aatgccattc ccgcccgtac
     2401 ttgcagcgta aagcagccag gaaacaaagg cttgtgctgt caagcctttt tccaggagca
     2461 attcatggtt tttggtgctt acccggtttc gtacgagtaa atccgataaa ttccattcca
     2521 tacccaccac ccccttttta agtctgtcgg attcagcttc taaatcagga tcaatgtcag
     2581 cgtcggtggt ggttggagtg tttttcttgt gattcaaacc aagtgctaag gtgtttaaac
     2641 tgtgccaatc gcgccaatcg ggtgtgtcac tcgcgccatt cttatgagtg tcaaccgcgc
     2701 catcatgtat gtcacttgcg ccaggcttat gtatgtcagg cgcgccatta tgtatgtcaa
     2761 tcgcgccaat gagatcggcg ctttgatcct ctctatgtgt gtcacttgcg ccaaccaaat
     2821 tcgggtcagt agcactgcta tgtgtgtcat tcgcgccaat gagatcgttg ccattttgag
     2881 ggttatgtat gtcacttgcg ccaattgcaa cggcagacaa agactcatta gcacgcgcct
     2941 ggtcactttc ggtcattggc tcacccaggc aaaccttgaa acggcggggt gcatcctccc
     3001 aggatccgat ttctcgacct gtttcccgca caaacgttgc gacttcacct gttcgcagcc
     3061 attcccagac cgtcttggca cgtttcaaac ccaaccaacg ggcggcttcg gcatatcctt
     3121 ctcgtaggtg aatttcatcc ctcacctcgc cggtgcgctg gttgatgtag ccgcgatcgc
     3181 gcatcagggt cacaaaccag ccaggaccgg gtcctaacct gggcagccag tgagaaacga
     3241 agtaatgggt cagaaagacc agatccttgg gtggcatcaa gtgatgtgcc aatttatccg
     3301 caaactcccg gaactccgcc ttttggcttt cagggatcga gccgcacaca gcctgaagca
     3361 catcttggac agaatgcggt tcatcaatgg cttcttccga ggccacctga tctggccagg
     3421 ggatcagctc atcgactggc gtctccaaag ctgattgaat tacagccacc ggcgtttttc
     3481 cttgagcaag ctgtcgataa agccaggagc gtaaagattg ttcatcaaat ggagtcagag
     3541 gtggattcat ggttacccgg taatatcgag aagattggtg cgggcgacca tctttgcccc
     3601 ggctccagcg tggttcacta tcgacttgct ttaccagcca gcgaaggcgc ttccatgtct
     3661 ctggtttgg
//
LOCUS       DATMXK010000144         2385 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_133036, whole genome shotgun sequence.
ACCESSION   DATMXK010000144 DATMXK010000000
VERSION     DATMXK010000144.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2385)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2385)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2385
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_133036"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..394)
                     /locus_tag="VN363_02665"
     CDS             complement(<1..394)
                     /locus_tag="VN363_02665"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83438.1"
                     /translation="MGERITRPRNKIRILLVDDHANLRAGVRRLLETTRDIIVVGEAS
                     DGYQALELVERLEPDIVILDVEMPRMNGLVAARKLSDRYESVKILALSAYDDRQYVLG
                     MLDQGASGYLIKDEVPEMLIQAVRDISLG"
     gene            complement(550..1182)
                     /locus_tag="VN363_02670"
     CDS             complement(550..1182)
                     /locus_tag="VN363_02670"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83439.1"
                     /translation="MAQIRVLLIDDHPIVRSGIRNLIENSIGIEVIGEASNGSNGLEL
                     IEKLQPDVVLLDMELPDIPGNEVALRLRDKGSTCAILALSAHDDRMYIQELLSSGAAG
                     YLMKEEIPEFIVDAIRGVARGERGWLSRKIAAQMTEWMQAGHQTPAEISSREKEVLKG
                     VVDGKTNQEIAFQLGISVKTVEKHLDGLFTKLGVASRVEAAVMAVREGWF"
     gene            complement(1185..>2385)
                     /locus_tag="VN363_02675"
     CDS             complement(1185..>2385)
                     /locus_tag="VN363_02675"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ83440.1"
                     /translation="MGATPSGEFMDLLGEIATQLGKFIERQQAHDQLREREEHLSQLV
                     ESVKDFAFYTLDLNGHITSWNKGAEKIKGYSRNEIIGKHFSIFYTPEDIAANKPALVL
                     ESALQEGGFNSESWRVRKDGTRFWANVIVFPIYNSAGILQGYSKLTRDLTKRKLMEEE
                     LIASQLRHEKETSELRRRLMEGREAERLGMAQELHDGPIQELYGLIFGLDAIQGKIRS
                     QAALEQIKDMKRELQKVVSTLRGMIGDLRPPTLAPFGLEKAIRSHMQSFRDAHGEIQI
                     EMELDVDRRTLPEDVRLALYRVYQSSMVNIVRHADASQVKVKFKLDDDQVRLEIEDNG
                     KGFMVPERWIKLARAGHLGLVGARERVEAVGGRFIVESMPGEGTQVKVYIPREAIDVE
                     TQDGSGR"
BASE COUNT          530 a          736 c          524 g          595 t
ORIGIN      
        1 ctcccagact gatatcccgc acagcctgga tgagcatttc tggaacctcg tctttgatca
       61 gatagcctga cgcgccctgg tccagcattc ccaatacata ctggcggtcg tcgtaagcgc
      121 tcaacgccag gatttttacc gactcatacc ggtctgagag cttgcgggct gctaccaatc
      181 cattcatgcg cggcatctct acatccagga taacgatatc tggctccagc cgttcgacca
      241 actccaatgc ttgataacca tcactggctt ctccgacgac gataatatct cgggtggtct
      301 ccagcagtcg cctcactcca gctcgaagat tagcatgatc atcaaccagc aagatgcgga
      361 ttttgttgcg aggtctcgta attctctctc ccatcgtttc ccatcaatct gttccactct
      421 tgacttccac cacactacgc cttggtagaa tcataaaaca aactttcgaa ttatgctatc
      481 gggaattatc cggatccgag tagggaaaac cctactttac aaaccctgaa aaaatggttc
      541 cgtcctgagc taaaaccagc cttcacgaac agccatcacg gcggcttcga cccgagacgc
      601 cacacccagc ttggtgaaca gaccatcgag gtgtttctca acggtcttta cgctgatccc
      661 cagttgaaac gcgatctcct gatttgtttt gccatcaaca actcctttca agacttcctt
      721 ctcacggctc gaaatttctg ccggcgtctg gtggcctgcc tgcatccatt cggtcatctg
      781 ggcagctatt ttccggctca accagccgcg ctcgccgcgc gctaccccgc ggatcgcatc
      841 cacgatgaac tcggggattt cttctttcat caaatagcca gcggcgcctg agctcagcag
      901 ctcctggata tacatgcgat catcgtgggc gctgagagcc aggattgcgc aggtagatcc
      961 cttatcgcgc aggcgcaggg cgacttcatt acctggaata tccggaagct ccatatccag
     1021 cagcaccacg tccggttgga gcttctcaat cagctccagg ccatttgaac cattgctggc
     1081 ttccccaatc acctcgatcc cgatcgaatt ctcaatcagg ttgcgaattc cactccggac
     1141 aatcgggtga tcatcaatca gaaggacacg aatttgtgcc atgtttacct tccactccca
     1201 tcctgcgttt cgacatctat ggcttcccgg ggaatgtaaa ctttcacctg cgtaccttct
     1261 ccaggcatcg actccacaat aaacctgccc cctacagctt cgacccgctc acgtgccccc
     1321 accaggccca gatgcccggc cctggcgagc ttgatccagc gctctggcac cataaagcct
     1381 ttcccgttat cctcgatctc aagccggact tggtcgtcat ccagcttaaa tttgaccttc
     1441 acctgactgg catcggcatg ccggacgatg ttgaccatag aggattggta gacccgatat
     1501 aatgccaggc gcacgtcctc tggcagtgtc cggcgatcca catccaattc catctcaatt
     1561 tggatctctc catgagcatc ccgaaaactt tgcatgtggg aacgaatcgc tttttccagg
     1621 ccaaacggcg ccagggtagg tggacgtaaa tcgccaatca tcccccgcag ggttgagacg
     1681 accttttgca gctcgcgctt catgtctttg atctgttcca gggcagcctg gctgcgaatt
     1741 ttcccctgga ttgcatccag accgaaaatc aagccatata attcctggat aggtccatca
     1801 tggagctctt gagccatacc caggcgctcg gcctctctcc cctccatcag tcgcctgcgc
     1861 agctcagagg tctctttctc atggcgaagc tgactggcga tgagctcttc ttccattaac
     1921 tttcgtttgg tgagatcgcg ggttaatttt gagtaaccct gtaatatacc cgcgctgtta
     1981 tagatcggga aaacgatcac attggcccaa aaccttgtgc catctttgcg cacccgccag
     2041 ctctccgaat tgaatccacc ttcctggagg gctgactcaa gaactaaggc aggtttattg
     2101 gctgcgatat cctctggcgt atagaaaatc gagaaatgct taccgattat ttcgttccgg
     2161 ctgtagcctt tgatcttctc agccccctta ttccagctcg tgatatggcc attcagatcc
     2221 agcgtataaa aagcaaaatc cttaacactc tcgaccagct gtgacagatg ttcctcgcgc
     2281 tcgcggagct ggtcatgagc ctgctgccgc tcgatgaatt tacccagctg cgtggctatt
     2341 tccccaagca aatccatgaa ctcaccagac ggcgtggctc ccatg
//
LOCUS       DATMXK010000145         2245 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_150827, whole genome shotgun sequence.
ACCESSION   DATMXK010000145 DATMXK010000000
VERSION     DATMXK010000145.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2245)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2245)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2245
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150827"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1545
                     /gene="ftsH"
                     /locus_tag="VN363_02680"
     CDS             <1..1545
                     /gene="ftsH"
                     /locus_tag="VN363_02680"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560485.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent zinc metalloprotease FtsH"
                     /protein_id="HWQ83441.1"
                     /translation="PSAILGVATALGYILPFILIAGVFYFVFRQAQGSNNAALSFGKS
                     RARMFTGDQPTVTFQDVAGVDEAKEELEEVVEFLREPEKFISLGARIPKGVLLVGPPG
                     TGKTLLAKAVSGEAGVPFFSISGSEFVEMFVGVGASRVRDLFDQAKRHSPCIVFVDEI
                     DAVGRQRGAGLGGSHDEREQTLNQMLVEMDGFDTDTNVIIVAATNRPDILDPALLRPG
                     RFDRRVTLDRPDMHGREEILKVHVKGKPLDPSVELAVLARSTPGFVGADLENLVNEAA
                     ILAARRNLKTIGQSELEEAIERVIAGPARKSRLISSEEKRIVAYHEAGHAVVMNALPS
                     ADPVHKVSIIARGMAGGYTLALPEDDRTLMPRKKMFTEMVSLLGGRAAEEIVFDDITS
                     GASNDLERVTRLARAMVTRLGMSEQLGPMVYGQKEELIFLGREISEQRDYSEAVAEQI
                     DHEVRRLVSEAHTKAHQILVEYRDRLDTIAERLLEVETISRDEFETLFPPPQPKTSGT
                     PLPLAA"
     gene            1637..>2245
                     /locus_tag="VN363_02685"
     CDS             1637..>2245
                     /locus_tag="VN363_02685"
                     /inference="COORDINATES: protein motif:HMM:NF013720.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83442.1"
                     /translation="MTAKPLPADYRAALEGIAWYRHHDPGCLSIQGRDQAAFLQRQTT
                     HDVRLLNGDRALPTILTSSNARILDVLYLLPGSEGNILALTLPGQGAATAAYFKKRIF
                     FMDQVAVEDLSAAYTQIDLIGPCREDILQPMGFTRAPTADEILTAEWEGCLVYLLHNS
                     APLWPGWRLFFPAGISTTIDRVLEQVGATQLTDENYHLLHLEN"
BASE COUNT          491 a          611 c          627 g          516 t
ORIGIN      
        1 cccagcgcta tccttggcgt tgccacagcc ctggggtata tcttaccatt catcttaatc
       61 gcaggcgttt tctacttcgt ttttcggcag gcgcaaggca gcaacaatgc cgccctttcc
      121 tttgggaaat cccgcgcccg catgttcacc ggtgaccaac ctacagtcac ctttcaggat
      181 gttgcggggg tagatgaagc caaagaagaa ttggaagaag ttgttgagtt cttgcgcgag
      241 cccgagaagt ttatttctct cggtgcccgc atcccgaagg gtgtgctgct ggttggcccg
      301 cctggaaccg gtaaaaccct gctcgccaaa gcggtatccg gcgaggctgg agtgcccttc
      361 ttctcaatct ctggttctga atttgtcgaa atgttcgtcg gtgtgggcgc aagccgggtc
      421 cgcgacctgt ttgatcaggc aaaacgccat tcaccttgta ttgtattcgt ggatgaaatt
      481 gatgctgtcg gtcgccagcg tggagccggt ctgggtggct cgcatgacga gcgtgaacag
      541 accctaaacc agatgctggt ggaaatggat ggcttcgata ccgacaccaa tgtgatcatc
      601 gtggcagcaa caaaccgccc cgatatcctg gaccccgctc tgctgcgccc cggtcgtttc
      661 gaccgccggg tgactctcga tcgcccggac atgcacggac gggaggaaat ccttaaagtc
      721 catgttaagg ggaaaccgct cgatcccagt gttgaattag cagttctggc acgctcgaca
      781 cccggctttg taggtgcaga tctggaaaac ctggtcaatg aagccgcgat cctggctgcc
      841 aggcgcaacc tgaaaacgat cggtcagtcc gaattagagg aggctatcga gcgtgtgatt
      901 gcaggcccag cccgcaaaag ccgcctgatc agctctgaag agaagcggat cgttgcctat
      961 catgaggccg gacacgcagt cgtcatgaac gccctgccct ccgctgatcc agtccacaag
     1021 gtgtcgatta tcgcccgagg tatggcaggt gggtacaccc tggctcttcc agaagacgac
     1081 cgtaccctga tgccgcgcaa gaaaatgttt accgagatgg tgagcctgct gggtgggcgc
     1141 gccgctgaag aaatagtctt tgatgacatc acctcagggg cttccaatga tctggaaaga
     1201 gttacccgcc tggcacgggc tatggtaaca cgcctgggta tgagtgaaca gcttggtccg
     1261 atggtttatg gacaaaagga agagctgatc ttcctggggc gcgagatctc ggagcagcgt
     1321 gattactcag aagcggtggc tgaacagatc gatcatgaag tacgccgcct ggtcagcgaa
     1381 gcgcatacca aggctcacca gatcctggtc gaataccgcg atcgcctgga tacaattgcc
     1441 gaacgattac tggaagttga gacgatctcc cgggatgaat tcgagacgtt gtttcctcct
     1501 cctcagccaa aaacgagcgg tactccgctt ccgctggcag cctgagactg atctagtttc
     1561 caatgtgtga ggcgcacgcc gcgagtgttg agctctgcgc agtgcgcctc actttttttc
     1621 caggaggtac cttggaatga ccgctaagcc tttgcctgct gattatcgag cagcgcttga
     1681 aggaattgcc tggtaccggc accacgaccc aggctgcctg agcattcagg gtcgcgatca
     1741 ggctgctttc ctccaacgcc agacaactca tgatgtgcgc ttgttaaatg gtgatcgggc
     1801 tctccctacg atcctgacct cctcaaacgc ccggatcctg gatgtgcttt accttctgcc
     1861 cggttcagag gggaatatcc ttgccctgac cctccctggt caaggggctg cgacagctgc
     1921 atattttaaa aagcggatct ttttcatgga tcaagttgca gttgaggatc tgagcgcggc
     1981 atatactcag attgatttga tcggcccttg ccgagaagac attctccaac ccatgggctt
     2041 caccagagcg cccacggcag atgaaatcct gactgcagaa tgggaaggtt gtctggtcta
     2101 tctgctccat aacagcgcac ccttatggcc cggatggagg ttgtttttcc cagccgggat
     2161 ctcaacgaca atagacaggg ttttggaaca ggttggtgcg acccagctga ccgatgaaaa
     2221 ctatcattta ctgcatttgg agaat
//
LOCUS       DATMXK010000146         3668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_48418, whole genome shotgun sequence.
ACCESSION   DATMXK010000146 DATMXK010000000
VERSION     DATMXK010000146.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48418"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..565
                     /locus_tag="VN363_02690"
     CDS             <1..565
                     /locus_tag="VN363_02690"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="dienelactone hydrolase family protein"
                     /protein_id="HWQ83443.1"
                     /translation="TNGGTVQDSLGFWKTAAAEGWITAAPQSSQAMWRGAYVWDDRGY
                     ARDELQRHYTSLIQKYAIDPQRVILAGHSLGGETAMWLALNAELPVQGFIAFGPSGPL
                     TEDPEKWHDLLYQSAGLGLRGYIIFGEEDESIPQENIRLLVDLLNETGTPTDLETVPH
                     AGHDYDPAYDDCLLRALEFIFPQGGKG"
     gene            complement(571..1173)
                     /gene="nadD"
                     /locus_tag="VN363_02695"
     CDS             complement(571..1173)
                     /gene="nadD"
                     /locus_tag="VN363_02695"
                     /EC_number="2.7.7.18"
                     /inference="COORDINATES: protein motif:HMM:NF000840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nicotinate-nucleotide adenylyltransferase"
                     /protein_id="HWQ83444.1"
                     /translation="MKIGVFGGTFDPPHIAHLILAQEACNQLGLERLLWVLTPNPPHK
                     RGRKMTAAPQRWAMLQLAVQDNPRFVPSRVDIDRPPPQYAVDTLHLLAQAYPKAELVY
                     LIGGDSLRDLPHWHRPLDVVAASAAIGVLHRPRTRIDLKKLEARLPGLSAKIRWIAGP
                     RLDISATHIRRLAGEGKEFRYYLPEAVYTYIRENQLYCPA"
     gene            complement(1186..2445)
                     /gene="obgE"
                     /locus_tag="VN363_02700"
     CDS             complement(1186..2445)
                     /gene="obgE"
                     /locus_tag="VN363_02700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559739.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase ObgE"
                     /protein_id="HWQ83445.1"
                     /translation="MFIDQADIDVRSGKGGDGSMHFRREKFVPRGGPDGGDGGRGGDV
                     VFEVLPTLNTLSSFRHKARYIAESGGNGGKQNMSGKSGQDLVIHVPPGTLVYSRDSGE
                     LLGDLVEPGQRLVIARGGRGGRGNSHFVSSVHQAPRIAERGAPAEERSLRLELKLIAD
                     IGIVGVPNAGKSTFLAAVTNARPKIAPYPFTTLEPNLGVAELDEETNLVLADIPGLIE
                     GAHQGIGLGHDFLRHIQRTRVLIHLLDGMAENPLLDFAQINSELALFDAHLAEKPQVV
                     AVNKIDLPDVQARWPELQKALQKKGYSAVSISAVAGTNVRQVLYQASQLLQEAPPVEV
                     VASIPVYRPEQDPNEFRIERESDGWRINGEAIERAASMTYWGEFQSIRRFQRILEHAG
                     IDEALREAGVKEGDTVYIGEYELEWSD"
     gene            2702..3322
                     /locus_tag="VN363_02705"
     CDS             2702..3322
                     /locus_tag="VN363_02705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559794.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="redox-sensing transcriptional repressor Rex"
                     /protein_id="HWQ83446.1"
                     /translation="MNGKMIPDIVVGRLPLYLRALQQMTNEGRQVTSSQELGERLGIS
                     AAQIRKDLSQFGEFGKQGTGYHIDYLLKMIRQILKVDRVWNMIVVGAGDIGSAVAGYQ
                     GFADRGFQVTMLFDNNPAKIGQQIGKFTVLDSATLAESIREAGIQVAMIAVPADHAQA
                     VAELLVSAGVKAILNYAPLNLMVPQDVRVQYIDPASHLQRMTYYLD"
     gene            complement(3326..>3668)
                     /locus_tag="VN363_02710"
     CDS             complement(3326..>3668)
                     /locus_tag="VN363_02710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559793.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="rod shape-determining protein"
                     /protein_id="HWQ83447.1"
                     /translation="IEIQGQDQVTGLPRPVTMTTGEVVEALHDPLGNIIETARRVLEK
                     TPPELVSDIIDRGVALTGGGAKLRGIDRLLTKELGVPAYLVDNPTSCTAEGAARGFSV
                     FGLIRRNLPRA"
BASE COUNT          756 a         1039 c         1113 g          760 t
ORIGIN      
        1 taccaacggg ggtaccgtcc aggattcact gggtttctgg aaaacggcgg cagccgaagg
       61 ctggattact gccgccccgc agtcctccca ggccatgtgg agaggcgcgt atgtatggga
      121 tgatcgcggt tacgcccggg acgagctcca gcgccactac acatctttaa tccagaaata
      181 tgccatcgac ccgcagcggg tgatcctggc agggcattcg ctgggcggcg agactgccat
      241 gtggctggcg ctcaacgctg agctcccggt ccaggggttc atcgccttcg gaccgtccgg
      301 cccgctcacc gaggatcctg agaaatggca cgatctgctg taccaatccg ccgggttggg
      361 tttgcggggc tatattatct ttggcgagga ggatgagagc atccctcagg agaatattcg
      421 cctgctggtc gacctgctca acgagacggg cacgcctaca gatctggaaa cggtgcccca
      481 tgccgggcat gattacgatc cagcctacga tgactgcctg ctgagggcat tagagtttat
      541 ctttccgcag ggcgggaagg ggtaagccag ttaagcgggg cagtagagct gattttcgcg
      601 gatgtatgtg taaacagctt cgggcaggta atagcgaaat tccttcccct ctcctgccag
      661 gcggcgaata tgggtcgccg agatatccaa ccgggggccg gcaatccagc ggatcttcgc
      721 cgagagaccc ggcagtctgg cttccaattt tttgaggtct atgcgcgtcc gcggccggtg
      781 caggacgcca attgccgcgc tggcggcgac aacatccagg gggcggtgcc agtggggcag
      841 atcgcgcagc gaatccccgc cgatgagata gaccagctca gcctttgggt aagcctgggc
      901 cagcaaatgc agggtatcca ctgcatactg cggaggtggg cggtcgatat cgacccggga
      961 ggggacgaag cggggattgt cctggacagc cagctgcagc attgcccagc gctgaggtgc
     1021 ggcggtcatt ttgcggccgc gcttgtgagg agggttggga gtgagcaccc acaagaggcg
     1081 ttccagcccg agctgattgc aggcttcctg ggccagaatg agatgagcga tatgaggcgg
     1141 gtcgaaggtg ccgccgaaaa ccccaatttt catcggtatt cgagcctaat cgctccactc
     1201 cagctcgtat tctccaatat aaaccgtatc cccctccttc acgccggctt cccgcagcgc
     1261 ctcgtcgatc cctgcatgtt ccaggatgcg ctggaaacgg cgaatggact ggaattcacc
     1321 ccaataggtc atggaagctg ccctttcaat cgcttcgccg ttgatccgcc agccatctga
     1381 ttcgcgctca atccggaatt cattggggtc ctgttccggg cggtaaaccg ggatgctggc
     1441 gaccacttcc accggtggcg cctcctggag cagctgggaa gcctggtaga gcacctggcg
     1501 gacattcgtt ccggcgacgg ctgagatcga aaccgcggaa taccccttct tttgtaaagc
     1561 cttctggagc tctggccagc gggcctgtac gtctggcaga tcaattttat tcactgccac
     1621 cacctggggt ttttccgcca ggtgggcgtc aaacaaggcc agctcgctgt tgatctgggc
     1681 gaagtcgagc aggggatttt cggccatgcc atccagcagg tggatgagga cgcgggtgcg
     1741 ctggatgtgg cgcagaaaat catgccccag accaatcccc tgatgggcgc cttcaatcag
     1801 gccgggaata tcggccagca ccaggttggt ttcctcatcc agctctgcga cgcccagatt
     1861 gggctcgagg gtcgtaaatg ggtaaggggc aatctttggc ctggcattgg tcactgcggc
     1921 caggaaagtg gatttgccgg cgtttggcac gcctacgatc ccaatatcag cgatgagctt
     1981 cagctccaga cgcaggctgc gttcttcggc tggagcgccg cgttcggcga tgcggggcgc
     2041 ctggtggaca gaggatacaa aatggctgtt cccccgcccg ccgcggccgc ctctggcgat
     2101 taccaggcgc tggcccggct caacgagatc accgagcagc tcgcctgagt cgcgcgagta
     2161 gaccagggtg cctgggggaa cgtggatcac cagatcctgg ccggatttac ccgacatgtt
     2221 ctgcttccca ccgttgccac cagactcggc tatatatctc gccttatgcc ggaaggacga
     2281 gagggtattc agagttggca gcacctcaaa aaccacatcg ccgccccgcc cgccatcgcc
     2341 gccatccggg ccgccgcggg ggacaaattt ttcgcgccgg aaatgcattg acccatcccc
     2401 gcccttgcca gaccggacat caatatccgc ttgatcgata aacataatct aatttccctg
     2461 gaatctgatt gagccagtca ccttaccact gcgccggctg aggtttgcaa caaaagatta
     2521 taccatccag aagtcattcg agactattcc agccccggga gaatggacag cctgcctgcc
     2581 gggggctccg gacctgtttg taccccactt gtacatttct tgtggaatat tgcacaatga
     2641 ccaaaaatgt ggtagattat agaagacaat ttgcagctta attttcggga gggcttgagg
     2701 catgaatgga aaaatgatcc ctgatatcgt cgttggacgg cttccactgt acttgcgcgc
     2761 tttacagcaa atgaccaatg aagggcgcca ggttacctcc tcgcaagagc tgggcgagcg
     2821 cctgggcatt tcggctgccc agatccgcaa ggacctctcc cagtttggcg aattcgggaa
     2881 gcagggtaca gggtatcaca tcgattacct gctcaagatg atccgccaga tcctgaaagt
     2941 agaccgggtg tggaatatga tcgtcgttgg ggccggtgat atcggcagcg ctgtggcagg
     3001 ctaccagggt tttgccgatc gtggttttca ggttacgatg ctgtttgata ataacccggc
     3061 taaaatcggc cagcagattg gcaaattcac cgtgctcgac agcgcgacgc tggcagaaag
     3121 tatccgcgaa gcggggattc aggtggcgat gatcgcagta cctgccgacc atgcccaggc
     3181 tgtagcagaa ttactggtaa gcgccggggt gaaggcgatc ttgaattatg cgccgctcaa
     3241 cttgatggtt ccgcaggacg tgagagtgca gtacatcgac cccgccagtc acctgcagcg
     3301 gatgacgtat tacctggatt gaagttcagg cgcggggtaa atttcgccgg atcaggccaa
     3361 agacgctgaa accacgcgcc gcgccttcag cggtacagga agtgggatta tcgaccaggt
     3421 aggcgggaac tcccagttct ttggtgagca gcctatcgat accgcgcagc ttcgccccac
     3481 ccccggtgag ggctaccccg cggtcgataa tatccgaaac cagctcggga ggggttttct
     3541 ccaggacgcg gcgggcagtt tcgatgatat ttccgagggg atcatgtaaa gcttcgacaa
     3601 cttcgccggt ggtcatcgtc accgggcggg gcagaccggt tacctggtcc tggccctgga
     3661 tttcgatg
//
LOCUS       DATMXK010000147         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_128094, whole genome shotgun sequence.
ACCESSION   DATMXK010000147 DATMXK010000000
VERSION     DATMXK010000147.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128094"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..678)
                     /locus_tag="VN363_02715"
     CDS             complement(<1..678)
                     /locus_tag="VN363_02715"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83448.1"
                     /translation="MPETRRYIDLLLQLRDLDPQTQTFKLAVLPNPGGPLGSVPAVSV
                     PALSEDDQFLLDDLERKNLTVDDAIKLGKALTERLLPDGPVRAIFHQALVLAGQDGGL
                     RLRLNIQSPDLAQIPWELVYDPEQLGENKTAYFLVLNPRISIARFEERLTPPPPLALK
                     DPARVSIRPVTANARVSGQRPLKLDQERKVIEESLANFEVDGVTIEIKPFVEQASWED
                     LTQALAGK"
     gene            complement(679..2157)
                     /locus_tag="VN363_02720"
     CDS             complement(679..2157)
                     /locus_tag="VN363_02720"
                     /inference="COORDINATES: protein motif:HMM:NF014503.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83449.1"
                     /translation="MPPNSHSASDGIPPHFIVVVPGYMGSLLRDSQDGQTLWVDIPGL
                     LRNPLNIAENVDHMLERLKYPNQDIVPAGLMDQVLFVPPFLKAEHYGRLLQALRRMGY
                     QEGNGGGTTNRPPVYTFAYDWRQDNRISARQLGQAIALWSDLHSPAKAWIIAHSNGGI
                     VSRWYIEKEGGKDFVERLFLMGSPWDGAPKALNVVLNGLDVFFVKLLNRYGIQQRIKD
                     LVRSFPSYYQLIPYSKPFVFDANNNPVDLFADPRWLGGDEDIRMLQEGLRFNQELGTT
                     LSVDTVCFFGRRKPTLTSAVVNIGAGGQWHDIDWIYTEAGDGTVPERSAVHPSVREAG
                     RAFAFAVDHGNIYVDPQVLVQLEFELVGRYQEATRATLFTDEFSIFFAPTQDTYLPGQ
                     IIDLKATISRNEPDNPPQSNAAIKVQMIWDQALPSQPEALAPAALPSATLLETTQQSG
                     EYSGKITAPTQEGYYRLLASVEIQSKPTLTLEELVSVEAESV"
BASE COUNT          565 a          618 c          618 g          534 t
ORIGIN      
        1 cttaccagcc agtgcctgag tcaaatcctc ccagctggcc tgctcgacga atggcttgat
       61 ctctatggtc accccgtcta cttcgaaatt cgccagcgat tcttcgatca ccttgcgttc
      121 ctggtctaat ttcaatgggc gctggcctga gacacgtgca ttcgcggtca ctggacggat
      181 cgacaccctg gcagggtcct ttaatgctaa aggtggtggt ggagttaacc gctcctcaaa
      241 ccgggcaatt gagatgcggg gatttaagac caggaaatag gcagtcttat tctcgccaag
      301 ctgctcggga tcataaacca gctcccaggg gatctgggcc aggtctgggc tttggatatt
      361 gagccgtaag cgcagaccgc catcttgccc tgccaggaca agggcctggt ggaatatcgc
      421 ccgcaccgga ccgtcaggga gtaagcgctc ggtaagcgct ttgccgagtt taatggcatc
      481 atcgacagtc aggtttttgc gctccaggtc atcgagcaga aattgatcgt cttcactcag
      541 cgcgggcaca ctgactgccg ggacactgcc cagaggtccg cccgggttcg gcaagacagc
      601 cagcttgaag gtctgggtct gtggatccag atctctaagc tggaggagaa gatcaatgta
      661 acggcgagtt tcaggcattt acacgctctc cgcttccact gaaaccagtt cttccaaggt
      721 gagtgttggc ttactctgga tctccaccga tgccagcagg cgataatacc cttcctgggt
      781 tggcgcggtg atttttcctg aatactcgcc tgattgctgg gtcgtttcga gcaaggtagc
      841 ggaaggaagc gcagctggag ctaacgcctc cggttggctg ggtaaagcct gatcccagat
      901 catttgcact ttgatcgcag cattggactg cggtggattg tccggctcat tgcggctaat
      961 cgttgccttc aagtcaatta tctgacctgg cagataagta tcctgcgtag gggcaaaaaa
     1021 tatcgaaaat tcatccgtaa ataaggtggc ccgggtagct tcctggtagc gccccaccag
     1081 ctcaaattcg agctgcacca gcacctgagg gtcgacatag atattgccat gatcgacagc
     1141 aaaggcaaac gctctaccgg cttcgcggac ggagggatgg acagcactgc gctccgggac
     1201 agttccatcg ccggcttcag tatagatcca gtcaatgtcg tgccactgcc cacctgcgcc
     1261 aatattgacc actgcagagg ttaaggtcgg cttacggcgt ccaaaaaagc acacagtatc
     1321 cacactgagg gtagtcccca gttcctgatt aaagcgcagc ccttcctgga gcatgcgaat
     1381 atcctcatct ccgcccagcc aacgcggatc tgcaaataaa tccaccgggt tgttgttggc
     1441 atcaaacacg aagggttttg aataaggaat cagctggtaa taggaaggga aagagcggac
     1501 cagatccttg atccgctgct ggatcccata ccggttgagc aatttcacaa agaacacatc
     1561 caagccattc aggaccacat tgagggcttt cggcgcccca tcccaggggg aacccatcag
     1621 gaacaggcgt tcaacaaaat ccttaccacc ctctttctca atataccagc gcgatacgat
     1681 ccccccattg ctgtgagcaa tgatccaggc tttggcagga ctgtgcaaat cgctccacag
     1741 cgcgattgcc tgacccagct ggcgagcgga aatgcgattg tcctggcgcc aatcgtaggc
     1801 aaaggtatag accggtggtc ggttggtcgt ccctcctcca ttcccctctt gataccccat
     1861 ccggcgtaaa gcctgaagca gacgaccgta atgttcagct tttaagaatg gtggaacaaa
     1921 taaaacttga tccatcaaac cggctggaac gatatcctga ttgggatatt tgagacgttc
     1981 cagcatatga tcgacattct cagcgatgtt taaggggttg cggagtaaac cgggaatatc
     2041 cacccacaat gtttgcccat cctgggaatc gcgcaatagg ctgcccatat agcctgggac
     2101 aacaacaatg aagtggggag ggattccatc actggcagaa tgactattgg ggggcatagg
     2161 gaacctcctg attcagatga cagtaaaggt agggaatgca tggggcggga aagtcaatga
     2221 tggaaactat acctgctgct ctgggtttcc cgccacagca tatattggag tttctatatc
     2281 cactatactg gattttgatt gctctgtcaa cgaatcaagg tatgagtttt acaac
//
LOCUS       DATMXK010000148         2324 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_24051, whole genome shotgun sequence.
ACCESSION   DATMXK010000148 DATMXK010000000
VERSION     DATMXK010000148.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2324)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2324)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2324
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24051"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1152)
                     /locus_tag="VN363_02725"
     CDS             complement(<1..1152)
                     /locus_tag="VN363_02725"
                     /inference="COORDINATES: protein motif:HMM:NF028046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain-containing protein"
                     /protein_id="HWQ83450.1"
                     /translation="MVKSIRWRIAIPYILLIVLVMLALGLILGNLIRQNYLTQLESEL
                     SSNAYLLSQALLAPLREPASPADLEALAQQWAQALDSRITIIDPAGNVLADSHAGQVE
                     IENHLNRPEVSAAIQNGMGSSTRFSRTVGYEQMYVAAAVRQPAGLLGVVRISIPLQVI
                     QQGQNSLIRTLIGVSLAATLAALLLAIGIAGAASRPLRELTHTAEQIAYAASAGVAGG
                     TSGDTSGAAQGVAHGDQKLVNYSARGDEIAHLTQAFHAMSRQLSSQIADLEGERTKLS
                     AVLAEISDGLLIVNPAGQIQTINRAAQELFMVSEKDATGRSLIEVLRLHQAVELAQAA
                     QETGQTQSAILEHPARQLTLQAIAIPRHSQDPGSPESGAALPPAAGVLLL"
     gene            complement(1154..1861)
                     /locus_tag="VN363_02730"
     CDS             complement(1154..1861)
                     /locus_tag="VN363_02730"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83451.1"
                     /translation="MPDRILIVEDEPALQDTLAYNLTRQGYLVETVGDGQAAISAARR
                     QPPDLILLDIMLPVLDGFEVTRILRQELNTPILFLTARDDEIDRILGLELGADDYMTK
                     PFSMRELLARVKAQLRRVRMIRAEASANTELAQREQLNFGDLVIDQTRREVRLNAIPL
                     NLKPKEYELLHFLAMHRGQVLTRDLILERVWGWEFSGGSRTVDVHVRWLREKIESDPA
                     EPHRLVTVRGAGYRFEG"
     gene            complement(2047..>2324)
                     /locus_tag="VN363_02735"
     CDS             complement(2047..>2324)
                     /locus_tag="VN363_02735"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015035 - protein-disulfide reductase
                     activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutaredoxin domain-containing protein"
                     /protein_id="HWQ83452.1"
                     /translation="VISPRGNKMLEPEIIVYGTRWCGDCLRTRRYLEKYAVPFQFIDI
                     DKDKVAEGFVLTTNHGIRSVPTIVFGDGSILVEPNNLQLKEKLHLAV"
BASE COUNT          463 a          652 c          751 g          458 t
ORIGIN      
        1 gagcagcagg actcccgcgg ccgggggcag ggcagcccca gactcagggg agccggggtc
       61 ctgggagtgg cgcgggatgg cgattgcctg aagagtgagc tggcgagcag ggtgttccag
      121 gatagcgctc tgggtttgcc cggtctcttg agccgcctga gccagctcca cagcctggtg
      181 gaggcgcagc acttcgatca acgagcgacc ggtggcgtct ttttcactca ccataaacag
      241 ttcctgagcc gcgcggttga tggtctggat ctgcccggcg ggattgacaa tcagcagccc
      301 atccgagatc tcagccagga cagccgataa tttcgttcgc tctccttcca ggtcggcgat
      361 ctggctggaa agctggcggc tcatggcatg gaaagcctgg gtcaggtggg cgatctcatc
      421 gccgcgggcg gaataattga ccagtttctg gtcaccgtgg gcgacaccct gggcggcgcc
      481 ggaggtgtca ccggaggtgc cgccggcgac gcctgcggaa gcggcatatg cgatctgctc
      541 ggcagtgtgg gtgagctcgc gcagggggcg gctggccgcg ccagcgatcc caatcgccag
      601 cagcagtgca gccagggtgg ctgccaggct tacccctatg agcgtgcgga taaggctgtt
      661 ctggccttgc tggataacct gtaagggtat cgagatccgc accacgccca acaaaccggc
      721 tggctgcctg accgccgcgg ccacatacat ctgctcgtac ccgactgtgc gtgaaaagcg
      781 ggtactgctg cccatcccat tctgaatggc ggctgacacc tcaggccggt tgagatgatt
      841 ttcgatctcg acctgcccgg catgcgaatc ggcgagcaca ttcccggccg ggtcaatgat
      901 ggtgatgcgt gaatccaggg cctgagccca ctgctgtgcc agagcttcca ggtccgcggg
      961 gctggccggc tcgcgcagcg gagccaggag ggcctgggag agcaggtagg cattggatga
     1021 cagctcactt tccagctggg tcaggtaatt ctggcggatg agatttccca ggatgagtcc
     1081 cagggcaagc atgaccagga cgatcaggag gatataggga attgcgatgc gccagcggat
     1141 ggatttaacc atctcagcct tcaaagcgat agccggcccc gcgcacagtg accagccggt
     1201 gaggttcagc agggtcagac tcaattttct cgcgcagcca gcgcacatgg acgtctactg
     1261 ttcggctgcc gccggagaac tcccagcccc agacccgttc caggatcaaa tcccgggtga
     1321 ggacctggcc gcggtgcatc gccaggaagt ggagcagctc gtattccttg ggtttcaagt
     1381 tcagggggat agcgtttaaa cggacctcgc ggcgggtctg gtcgatgacc agatcgccga
     1441 aattcagctg ctcgcgttgg gctaactcgg tattggcgct ggcttcggcg cggatcatac
     1501 gcacccggcg cagctgggcc ttcacgcggg ccagcagctc gcgcatcgaa aagggcttgg
     1561 tcatgtagtc atccgcgccc agctccagcc cgaggatgcg atcaatctcg tcgtcgcggg
     1621 ccgtgagaaa caggatcggc gtgttcagct cctggcgcag gatgcgagtg acttcaaagc
     1681 catccaggac aggcagcatg atatcgagca gaatcagatc gggcggctgg cggcgggctg
     1741 cggaaatcgc cgcctggcca tcgccgactg tttccaccag gtacccctgg cgagtgaggt
     1801 tataggctaa agtatcctgc aaggcaggct cgtcttcaac gatgagaatg cggtcaggca
     1861 tagggtatgc gcttttaacg aactggtagg gtgttttccg ggatggtcga agaccagccg
     1921 ggctgttccc tgggttaggc ggggtcacag cgatgggatc ttgttaaaca gacccatctc
     1981 gtgtacatgg ggcgctcacg agtaatcgag taaccaccgc gatggtacaa ttatccggta
     2041 agggaattat accgccaggt ggagcttttc ttttagttga aggttgttgg gttcgaccag
     2101 aatactgcca tcaccaaaga cgatcgtcgg cacgctacgg atgccatggt tcgtcgtcaa
     2161 aacaaagccc tccgctactt tatccttgtc gatatcgata aactggaagg ggacggcata
     2221 cttttcgaga tagcggcggg tgcgcaggca gtcgccacac cagcgggtac catatacaat
     2281 tatctcaggc tcaagcatct tattccctcg gggtgatata acgt
//
LOCUS       DATMXK010000149         2086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_59546, whole genome shotgun sequence.
ACCESSION   DATMXK010000149 DATMXK010000000
VERSION     DATMXK010000149.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2086
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59546"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..772)
                     /locus_tag="VN363_02740"
     CDS             complement(<1..772)
                     /locus_tag="VN363_02740"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561284.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfite exporter TauE/SafE family protein"
                     /protein_id="HWQ83453.1"
                     /translation="MQHDIHLQRQLTLEIINYLWVALAALAAGAVNALAGGGTLITFP
                     ALIAVGIPAVAASMTNTVALCPGYFGATYAQAKDLKGQEKRLKLLMPAGAVGGVIGGL
                     LLANTGEKLFSALVPFLILLASGLLAIQDPLRAWLLRRSKQNGAESVPETWSWLPVGL
                     AAIYGGYFGAGLSVIVLAVLGLTLEDTLTRLNGLKQAISLSVNVAAAVFFLFSGQVVW
                     GVALVMAVGSLLGGVLGGRLASRIKPSVLRWTVVVIGVV"
     gene            complement(809..1783)
                     /locus_tag="VN363_02745"
     CDS             complement(809..1783)
                     /locus_tag="VN363_02745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83454.1"
                     /translation="MDSLTEWLLAGPPWVAYRTRLDLLGEAIDNPEVQQSRQAMLKHP
                     QVQQLVAELADWPGSLLSSHKSAGHAMHKLTFLADLGLKVSDPGVEQVVERVLEHRAD
                     TGPFQMLMNISPAHGGSGKDEWAWALCDAPLLVYALVNFGLGDDPRVTAALAHLIGLI
                     TERGWPCAVSPELGKFRGPGRKDDPCSYANLVMLKTLALRSEWQSLPASRTGIETLLS
                     LWESRSESHPYMFYMGTDFCKLKAPLVWYDILHVTDVLTRFPWLAGDPRMQSMLDIIR
                     SKLDRNGQLVPESIWTAWKEWDFGQKKEPSRWLTVLVQRVIHRLPAFA"
     gene            complement(1771..>2086)
                     /locus_tag="VN363_02750"
     CDS             complement(1771..>2086)
                     /locus_tag="VN363_02750"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024157.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ83455.1"
                     /translation="SEYDSAEDFVERGIGYVSIQQGEILGAAFASLVNSRGIEVSIYV
                     EEAHRRQGLATALASALLISCLERRLEPHWDAANPESCRLASKLGYQFQGSYEAYYLW
                     TH"
BASE COUNT          502 a          650 c          527 g          407 t
ORIGIN      
        1 ctaccacgcc gatcaccaca accgtccaac gaaggacaga gggttttatc cggctggcaa
       61 ggcgcccacc caaaacgcct cccaaaagcg agccgactgc catcaccagg gcgacacccc
      121 agaccacctg cccagaaaag aggaagaata ctgccgcggc cacattgacg ctcagtgaaa
      181 tggcctgctt caacccgttc aaacgggtca gggtatcttc cagggtaagg cccaaaactg
      241 ccagcacaat gacgctcaat cctgccccaa aatacccccc ataaattgca gccagcccaa
      301 ccggcagcca ggaccaggtt tcgggcacag actcggctcc gttctgcttc gaacgccgca
      361 ggagccaggc gcgcagggga tcctggatcg ccagcaagcc cgaggcaagc aggatcaaaa
      421 acggaaccag agcgctgaac aatttctccc cggtattcgc cagcagcaaa ccgccgataa
      481 ccccacccac cgctccggcg ggcatcagaa gtttcaggcg cttttcctgc cctttcagat
      541 ccttggcctg agcataggta gccccgaaat aaccgggaca taatgcgacc gtgttggtca
      601 tgctggctgc caccgccgga ataccgacgg cgattaatgc tggaaacgtt atcaatgttc
      661 cgccgcctgc cagagcattg actgcgccgg cggcgagagc tgccagagcc acccatagat
      721 agttgatgat ctccaatgta agctgccttt gtaaatgaat gtcgtgctgc acctggcatt
      781 cagcctgata atccccaatt taccacaact aggcgaaggc tggtaaccgg tgtattaccc
      841 tctgaactaa cactgtcaac caacgcgatg gctctttttt ctgtccaaaa tcccattctt
      901 tccaggcggt ccagatcgat tcggggacga gctggccatt ccgatcgagc ttgctgcgaa
      961 tgatatctaa catggactgc atccggggat caccggcaag ccaggggaag cgggtcagga
     1021 catccgtgac atgcaaaata tcgtaccaaa ccaacggcgc tttgagcttg cagaaatcgg
     1081 ttcccatata aaacatgtac gggtgacttt cggaacggct ttcccacagg ctgagcaggg
     1141 tttctatgcc agtacggctg gctggaaggc tctgccattc tgaacgcagg gccagggtct
     1201 tgagcatcac caggttggca tacgagcagg gatcatcctt tcgccctggc ccacgaaatt
     1261 ttcccagctc tggagatacg gcacagggcc agccgcgctc ggtaatcaat ccaattaagt
     1321 gcgccagggc tgcagttact cgaggatcat ctcctaagcc aaagttgacc agcgcataaa
     1381 ccagcaacgg cgcatcacac aaggcccagg cccattcatc tttcccactc ccaccatgcg
     1441 caggagaaat attcatcaac atctggaaag gacccgtatc agcccggtgt tccagcaccc
     1501 gctcgacaac ctgttcaact cccggatcgc tcactttcaa tcccagatcg gccagaaatg
     1561 tcaatttgtg catggcatgt ccggcgcttt tatgagaaga cagcagtgag ccgggccaat
     1621 ccgccagttc agctaccagc tgctgcacct gcgggtgctt cagcatcgcc tggcgggact
     1681 gctgtacttc ggggttatca atggcttcac ccagaaggtc taaacgtgtc cggtaggcca
     1741 cccagggcgg gcctgccaat aaccactctg ttaatgagtc cataagtaat aagcctcata
     1801 actcccctga aattgatagc ccagcttgct tgccagacgg caggactcgg gattggcggc
     1861 atcccaatgc ggctccagcc gccgctccag gcacgagatg agcagcgcac tggcgagggc
     1921 agtcgccagg ccctggcggc ggtgtgcttc ttcgacgtaa atacttacct caatgccgcg
     1981 gctgttcacc aggctggcaa atgctgctcc caggatctcg ccttgctgga tggatacata
     2041 cccaattccc cgctccacga aatcttccgc tgaatcgtat tccgag
//
LOCUS       DATMXK010000150         2389 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_145533, whole genome shotgun sequence.
ACCESSION   DATMXK010000150 DATMXK010000000
VERSION     DATMXK010000150.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2389)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2389)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2389
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145533"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(19..552)
                     /locus_tag="VN363_02755"
     CDS             complement(19..552)
                     /locus_tag="VN363_02755"
                     /EC_number="4.2.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558916.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008929 - methylglyoxal synthase activity
                     [Evidence IEA];
                     GO_process: GO:0019242 - methylglyoxal biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylglyoxal synthase"
                     /protein_id="HWQ83456.1"
                     /translation="MTEETSDSLPVKKYIALIAHDARKDDILEWVKYNIPTLQEHDLC
                     ATGTTGKMIVEKTGLNVTRFRSGPLGGDMQIGGKIADGEIDLLIFFWDPLEPQPHDPD
                     VKALLRIAVLYNIPTACNRATADFLISSPLLNSTYARNLPDFDKVIDEIREEVEDEAD
                     DVQGEEDAKDDMGEKDD"
     gene            complement(642..1826)
                     /locus_tag="VN363_02760"
     CDS             complement(642..1826)
                     /locus_tag="VN363_02760"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008233 - peptidase activity [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Xaa-Pro peptidase family protein"
                     /protein_id="HWQ83457.1"
                     /translation="MSELVKEKVSQAVQILQEKNIDCWLTFVRETSAGGDPVLPLIYG
                     LDLTWYSALILTRTGERYAIVGAFEAEAARRTGAYTMVIPYHEAISRSLLHTLERINP
                     SKIAINISEDDVLADGLTVGMYRTLLSFFDGTPWEQRLVSAEEVIRALRGRKMPGEVS
                     RLRKAIQTTEEIFARTFAYVQPGMSEIQIGEFMHAQINAAGLQPAWDWLDCPGVNAGP
                     DSSVGHVGPSPIELQRGHLLHIDFGVKKDGYCADIQRMAYYLAPGETQPPEPVQRGFD
                     TVMRAIQAALKAMKPGVTGQAVDQIARDLVTGEGYSEFKYATGHQLGQFTHDGAGVLG
                     PAWERYGKTPFYPLEAGQVYTIEPGLAVPGYGYIGLEEDVLVTERGAVFLSDPQMELI
                     VK"
     gene            complement(2071..>2389)
                     /locus_tag="VN363_02765"
     CDS             complement(2071..>2389)
                     /locus_tag="VN363_02765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83458.1"
                     /translation="LVVENAGFLPTMTSEQAKKRKAVRPVRAELALADGNMLVSGKVR
                     QELGHLEGRSNKWEGFFARNSSPTDNRARVEWVVQGKPGDKLKVKVLSERAGSLEKEV
                     ELS"
BASE COUNT          453 a          759 c          643 g          534 t
ORIGIN      
        1 cagtgcagaa ttcacacatc agtcgtcctt ctcgcccata tcgtctttcg cgtcctcctc
       61 gccctggacg tcgtcagcct cgtcttcgac ctcttcgcga atttcatcga ttactttgtc
      121 gaaatcaggc agattacgcg cataggtgga gttcagcagg ggggacgaga tcaagaaatc
      181 cgctgtggcc cggttgcagg cggttgggat gttgtacagc acggcgatac gcagcaaggc
      241 tttcacgtcc gggtcgtgcg gctgcggctc cagcggatcc caaaaaaaga tcagcaggtc
      301 gatctcacca tcggcgatct tcccgccgat ctgcatatca ccgcccagcg gcccagagcg
      361 gaaccgggtt acattcagcc cggttttttc gacgatcatc ttgccggttg tccccgtggc
      421 gcacagatca tgttcctgga gggtagggat attgtatttt acccattcca ggatgtcgtc
      481 cttgcgggcg tcgtgagcaa tcagggcgat atattttttt acgggcaggg aatcagaggt
      541 ttcttccgtc atagtcgttt acctttcaat tacatgcctc agacctcagg ggtctaaatc
      601 cttcagattt ttgaggcccc tgaggtctgg gtagagcagg attatttcac gatcaattcc
      661 atctgcgggt cgctgaggaa gacagcccct ctctcagtca cgagcacatc ttcttccagc
      721 ccgatatagc catagcccgg cactgccagc cccggttcaa tggtgtacac ctgcccggct
      781 tccagcgggt agaagggcgt cttgccgtag cgttcccagg ctggtcccag cactccggcc
      841 ccatcgtggg taaactggcc cagctggtgc ccggtcgcgt atttgaactc ggagtagcct
      901 tccccggtca cgaggtcgcg ggcgatctgg tccactgcct ggccggtgac acccggcttc
      961 atcgccttca gggctgcctg gatggcgcgc atcaccgtat cgaaaccgcg ctgcaccggc
     1021 tcaggaggct gcgtctcacc aggcgcaagg taatatgcca tccgctggat atcggcgcag
     1081 tagccgtctt tcttcacccc gaagtcgatg tgcagcaggt gaccgcgctg cagctcgatc
     1141 gggctgggac cgacatgccc caccgatgaa tcaggtccgg cattcacccc cgggcagtcg
     1201 agccaatccc aggcaggctg caggccggcc gcgttgattt gtgcgtgcat aaactcgccg
     1261 atctggattt cactcattcc cggctggacg taggcaaagg tccgggcgaa gatctcttcc
     1321 gtcgtctgga tggctttgcg cagcctgctc acttcgcccg gcatcttgcg cccgcgcagc
     1381 gcccggatca cttcttccgc cgagaccaat cgctgctccc agggcgtgcc atcgaagaag
     1441 ctcagcaggg tgcggtacat gccaacggtg agcccgtctg ccagcacgtc atcttcagag
     1501 atgttgatgg cgatcttact ggggttgatt ctctccaggg tgtgcagcag tgagcggctg
     1561 atcgcctcgt ggtaagggat caccatggtg taagcgccgg tgcgccgggc ggcttccgcc
     1621 tcaaaagcgc ccacgatggc gtaccgctct ccggtgcggg tcaggatcag ggcactgtac
     1681 caggtcagat ccagcccgta gatcagcggc agcaccgggt cacccccggc ggatgtctcg
     1741 cgcacgaagg tcagccagca gtcaatattt ttctcctgca ggatctggac cgcctgactg
     1801 actttttcct ttaccagctc agacatagct ccctccatcc taagattcac catacaataa
     1861 gaaacgagaa agtacccctg ccaaaaggat caaaataatg tcgcagcgag cgccaaattt
     1921 cctgtcattg cgaacgccag aattcctgtt actacgagca aagcgaagca atctggcgcc
     1981 agcctacctt gatacattct acccagcatt cagattgctt cagtcgttcc ggcgcaagac
     2041 cggtgcctcc actccttcgc aatgacatcc tcaggataat tctacttcct tttccagcga
     2101 gccggcccgc tcgctgagca cttttacctt cagcttatcc cccggcttgc cctgcaccac
     2161 ccactcaacg cgggcgcggt tatccgtcgg cgagctgttc cgggcgaaga aaccttccca
     2221 tttgttgctg cgcccctcga ggtggcccag ctcctggcgc actttcccgc tgaccagcat
     2281 attcccatct gccagcgcca gctcagctcg caccgggcgg actgctttgc gctttttagc
     2341 ctgctcgctg gtcatcgtcg gcaggaaacc ggcattttcc accaccagc
//
LOCUS       DATMXK010000151         5969 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_28448, whole genome shotgun sequence.
ACCESSION   DATMXK010000151 DATMXK010000000
VERSION     DATMXK010000151.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5969)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5969)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5969
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28448"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN363_02770"
     CDS             complement(<1..407)
                     /locus_tag="VN363_02770"
                     /inference="COORDINATES: protein motif:HMM:NF020250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SAF domain-containing protein"
                     /protein_id="HWQ83459.1"
                     /translation="MEKRRSPWIAIILAIVIGLMVIVLLNGYIRPTSVVVARVAIAPG
                     TRLTADLLELRTIPMQAKPRDAFEHIEDLADKILVVGRAPGDYIVASALGESAQAGIP
                     ANLQADHLAVAVRVDLASGIAGLLREGQTVTLIG"
     gene            complement(430..3882)
                     /locus_tag="VN363_02775"
     CDS             complement(430..3882)
                     /locus_tag="VN363_02775"
                     /inference="COORDINATES: protein motif:HMM:NF033849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="serine-rich protein"
                     /protein_id="HWQ83460.1"
                     /translation="METGSFQSHVRNIRDWLPRMPEWLSPHRAKPPVIPDDLEVERSG
                     VSFVDGRDELDYLWYRLREREGGREYAGYRVVRLLQLSFLPLESRADPGLLQKMRTVL
                     RGLYGSQVSFLYLAAGIFNHQQSPVGIVQCYGVAAFAPTLEEASAQSARSLAALKGAM
                     GGAYRQIRLEPLTVRLAEWIFLSFHDMQHVLVTVGHTDPRENARGGNSTLLRNPLTEA
                     GPTAQQYTLQQNEILFRGMSDLKEEFLFVVLTSPISLADITEMLTGLAEETSTWAAWQ
                     SGVRAVSFGVSLPALLSGLMAQSASQGYSTSQGSAHSEGQAHTDSQAHTDGVAHTEGQ
                     AETSGWSHSRSESESRSVGVAYSEGQAHTVGHAVTDGTAVTESSGHVSSRGSGSSHVD
                     SFNWGLRGGIQPAGVGIGGNVGWGSADGVSSMASESDMQGHSETASHAETESQADTVS
                     QGTVVSEVLTTTKGESWGTFGSQTHSQSDTVSQADTVGQADTKSSAESQMQSQSAGQA
                     IGRAASAGLAVGIAPSISASNSYQWQFDPAILVTQILRQQQNLLNLASKEGAYYTDVY
                     AMARTEQGKQALLGLIPEAFHGTEDVVTGVQTRDLTPEEGDYIGLHARAFTPSTRVET
                     IPEVVSGYMDSTILTMLQLAAYTAPGMFEQGTALTVQESTPDFAFRPAMAGDVVIGWQ
                     WSSETGQLTHTPLRLTPDRHFHTAFCGDTGFGKSVAAERLACETTRQWCFRSVVLDFG
                     QGWRRALNWAGLQGRVDIRQLYPGAPRPLRWNILKVPRRMYPSRYRTLVSELFANAGR
                     MGPRQLGFMRRALTQVYTNLGILTTDPEVINHPAWGHLQDSVEVAVAQEWRDRNRVTG
                     TVTIGQSLTDLQPGELQALAVWRSQRADVRMWVERLKNFYATLPKGDQASRASLEGVL
                     LRVEQFAEGQMAWQYGAGSESLPVEDLGLLGPEEDPWGLVIVEGGAEMDEYPKAALLA
                     LLASVLYFDSVVRRRESLAGAQFPPMQIFFEEANKVLTGVSGAAADQNASGNQTEQVS
                     EVFQTFWRDGRKYKIFLHLIAQTVSALPAGILSSCNNGFFFQTKHPHDRDLIMAHIGR
                     SEKGIVNTEYKRYLARIPKGLSIGKLGYSEDVAELEPMLVHPLMVPGSEPSDQEILER
                     LGAY"
     gene            complement(4010..4234)
                     /locus_tag="VN363_02780"
     CDS             complement(4010..4234)
                     /locus_tag="VN363_02780"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83461.1"
                     /translation="MDIYTLGRLALPGVVAIMAVAAVLTLFAGVFTAVKTLSSLQRFF
                     QQMEDSRRPSVTININTGRRASKPPMQAKK"
     gene            complement(4473..5171)
                     /locus_tag="VN363_02785"
     CDS             complement(4473..5171)
                     /locus_tag="VN363_02785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83462.1"
                     /translation="MNELDRIGMFLMDLPVYLANGGLVIAYWLAENSAVLVSLLCAIL
                     LALLPDAEIQQRAGFRPRRNDRSQNNGSINRLPRTAQTMTVIVFTVWLIAQWDMGAPV
                     PWIGAAMWVGGLLAVLALSAQRFNLLWYVKAGIAIYALAVIGSRIYLNYTAHLTPEQW
                     AALIGSSENAASVIASTRGNVTTILLWALWLVVPLGYFSMLVQQVFINPMSVVNPLAG
                     AQDLLRALRVRDTQ"
     gene            complement(5168..>5969)
                     /locus_tag="VN363_02790"
     CDS             complement(5168..>5969)
                     /locus_tag="VN363_02790"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83463.1"
                     /translation="LYLNGVADCASLPGGCAGLVADCSDGSCVPETPICPAPTISQAP
                     ATLAIQKAAPENPLVVGQDPEKRGADIQASASIPPVIFTWYEQVQDPPTCESASSGNG
                     EGCPGPGSRYDNNWEPSMEDNPAYEVVDGEIHCIEHVEILPEPITEVQANAQLSPESR
                     YWILNDLASKYYEAYVHQDRFDLLPGMATVNAGCGGNGTCTAEAFVPNVPFADPGAFD
                     LRLWVYTAGTAFNWNGVAIPITQPRVLFVQDTMQVYVTLVTLLPAGAP"
BASE COUNT         1299 a         1767 c         1671 g         1232 t
ORIGIN      
        1 ccgattaagg tcacggtttg cccctcgcgc agcaatccag caatgccgct ggccaggtcg
       61 accctgactg ccaccgccag gtgatccgcc tgcagattgg cagggatgcc ggcctgagcg
      121 gactctccca gagcagaggc gacaatgtaa tcgccaggag cacggcctac aaccaggatt
      181 ttatcggcca aatcttcaat atgctcaaaa gcgtcacgcg gcttagcctg catggggatc
      241 gtgcgcagct cgagcaggtc ggccgtcagg cgcgttcccg gagcaattgc cacccgggca
      301 accactaccg aggttgggcg aatataccca ttcaacaaga cgataaccat caggccaatg
      361 acgatcgcca gtatgatggc aatccagggg gaacgtcgtt tttccatatg catttacctc
      421 ctctgtttgt caataagcgc ccaaccgttc caggatctcc tggtcggacg gctcgctacc
      481 cggcaccatc aatgggtgca ccagcatggg ttccaattcg gccacatcct cgctgtaccc
      541 cagcttgccg attgacaggc ctttggggat gcgcgcaaga tagcgtttgt actcagtgtt
      601 gacgatacct ttctcgctgc gaccgatatg cgccatgatc aaatcccggt cgtgggggtg
      661 cttggtctgg aagaaaaatc cgttattgca tgatgacagg atgccagccg gtaaagcgct
      721 gaccgtctgg gcgatcaaat gcaagaatat cttgtactta cgcccatccc gccagaaggt
      781 ctgaaagact tcgctgacct gctcggtttg gttaccggag gcgttttggt ccgcggctgc
      841 gccgctcaca ccggtcagga ctttattagc ctcttcgaag aagatctgca taggcgggaa
      901 ctgcgctccg gccagactct cacgccggcg aacgacgcta tcgaaatata ggacgctggc
      961 aagcaacgct aataaggctg ccttggggta ctcatccatc tccgcgccgc cttcaacaat
     1021 gaccaaaccc caggggtctt cctcaggccc caacagcccc aggtcttcaa ccggaagact
     1081 ttcagagccg gccccatact gccaggccat ttgaccttcg gcaaactgct ccactcgcag
     1141 caggacgccc tccaggctgg cgcggctggc ctggtcgcct ttgggcagcg tggcatagaa
     1201 gttcttcaac cgttctaccc acatgcgcac atccgcgcgc tggctacgcc acaccgccag
     1261 agcctgcagc tcgcccggct gtaaatccgt gagcgactga ccgattgtga ctgtaccagt
     1321 tacccgattt cgatctcgcc attcctgggc aacggcaact tcgacgctat cctgcaaatg
     1381 accccaggca ggatggttga tcacttccgg atcggtggtc aggataccca gattggtgta
     1441 cacctgagtc aatgccctgc gcataaagcc aagttgcctt ggacccatac gcccggcatt
     1501 agcgaataac tcactgacca gcgtacggta gcggctgggg tacatccggc gcggcacttt
     1561 gagaatgttc cagcgcagcg gtcgcggcgc gcctggataa agctggcgaa tatcgacgcg
     1621 gccctgaaga cccgcccagt taagcgcccg ccgccagccc tgcccgaagt ccagcaccac
     1681 gctgcgaaaa caccactggc gggtagtctc gcaagccagg cgctcggcag ccacgctctt
     1741 tccaaagcca gtatcgccgc agaatgcagt gtggaaatgc cgatcaggcg tcaggcgcaa
     1801 tggggtatgg gtgagctggc ctgtctcgct gctccactgc cagccaatga ccacgtcacc
     1861 ggccatcgca ggccggaagg caaaatcagg cgtagattcc tgcacagtca gggcggtgcc
     1921 ttgttcgaac atgcctggag cggtgtaagc agcaagctga agcatggtca ggatggttga
     1981 gtccatataa ccactgacca cctcggggat cgtctccacc cgcgttgagg gcgtaaaagc
     2041 ccgagcatgc agaccaatgt agtcaccttc ttctggggtt aagtcgcggg tttgaacgcc
     2101 ggtgaccaca tcttccgtgc catggaacgc ttcggggatc agccctaaaa gcgcctgctt
     2161 accttgctcg gtgcgcgcca tggcatagac atcggtgtag tatgcgcctt ctttactggc
     2221 caggttgagc agattttgtt gctggcgcag gatctgggta accaggatcg ccgggtcgaa
     2281 ctgccattga tagctattag aagctgagat ggatggcgca ataccaactg ctaacccggc
     2341 tgatgctgcc cgtccaatcg cttgcccagc agattgtgac tgcatctggc tttcagcaga
     2401 gcttttggta tccgcttgcc caactgtatc ggcctggctg actgtatccg attgcgaatg
     2461 tgtctggctg ccaaaagttc cccatgattc acctttggtg gtggtcaata cttcactgac
     2521 cactgtgccc tggctgacgg tatctgcctg gctttccgtt tctgcatggc tggctgtctc
     2581 ggaatggcct tgcatgtcac tctctgaagc catgctagag acgccatctg ccgaacccca
     2641 gccgacattt cccccgatgc ctacgccagc cggttggata ccgccacgca gcccccagtt
     2701 gaagctatcc acatggctgc tgcccgaccc gcgcgagctg acgtgaccgc tgctttccgt
     2761 gactgccgtc ccatccgtca cggcatgccc gacggtatgc gcctggcctt ctgaataagc
     2821 cacccctacc gacctggatt cggactcgct ccgagagtgt gaccagccgc tcgtttcggc
     2881 ctgcccttct gtgtgggcga cgccatcagt atgcgcctgg ctgtcggtgt gggcctgccc
     2941 ttcactgtga gcgcttccct ggctggtgct gtatccctga gaagcggatt gcgccatcag
     3001 cccggagagc agcgctggca ggctgacgcc aaaggagaca gcacgcacgc cgctttgcca
     3061 ggctgcccag gtcgaggttt cttctgccag cccggtaagc atttcggtga tgtccgccag
     3121 gctgatcggc gaggtgagca ctacgaagag aaactcttct ttcaagtcgc tcatgccgcg
     3181 gaacaagatt tcgttctgct gcagggtgta ctgctgagca gtagggccag cctcggtaag
     3241 cgggttgcgc aacagggtgc tgtttcctcc gcgggcattt tcgcggggat cggtgtgacc
     3301 gaccgtgacc aacacatgct gcatatcgtg aaagctcaag aagatccact ctgccaggcg
     3361 aacagtgagt ggctccaggc ggatctggcg gtaagcccca cccatcgctc ccttgagagc
     3421 tgccaggctg cgcgccgatt gagcgctggc ttcctccaac gtgggagcaa aggcggcaac
     3481 gccataacac tgcacaattc ccactgggga ttgctggtgg ttgaaaatgc cagctgccag
     3541 gtacaagaag ctgacctgcg agccgtataa acccctcagc acggtgcgca ttttttgcag
     3601 caagccagga tctgcacgtg attccagtgg cagaaagctg agctggagca gccgcacaac
     3661 acgatagccg gcatactcac gcccgccctc ccgctcacgc agccggtacc acaagtaatc
     3721 cagctcatcc cggccatcta cgaaactaac gccggagcgt tcaacctcca gatcatctgg
     3781 aatgacaggc ggctttgcac ggtgtgggga taaccactcc ggcatacgcg gcagccagtc
     3841 acgaatattg cgaacatgag attggaagct tcctgtttcc atcagcttcg tctgctccga
     3901 attatgagct cacgcctgcc tgctggattg gcaggcgtca gttcacaata gataagatga
     3961 ggaaattcag gcgcacccat gctgcgcccg agcgtgtcgt gaagcaagct catttctttg
     4021 cctgcatggg aggtttggaa gctcgccgtc cagtattgat attgatcgtg actgaaggtc
     4081 gcctgctgtc ttccatctgc tggaagaaac gctgcaaact cgacagggtc ttcactgcgg
     4141 tgaatactcc ggcaaacagc gttaaaacag cggctacagc catgatcgca accactccag
     4201 gtaaagcaag tctcccaagc gtgtaaatgt ccataccatc ctccgtgtaa aaaaggggcc
     4261 aacgtgtggg gattggtttc caacgttcgg ttgcccagcc gacgttggtg ctgcccatta
     4321 cagaacagag tctgcgcaga gcaacacata cccgaggtga aatcgactaa aacgagagaa
     4381 ttcatcacag gcatgtggtg ctctgattcg agggcattcg agttacgagt catttcgggt
     4441 atggaaaatt caaaataagc attagcccta tgttactgtg tgtctctaac tctcagagcg
     4501 cgcagcaaat cctgcgcgcc tgccagagga ttgacgacag acattgggtt gataaaaacc
     4561 tgttggacca gcatggagaa gtaacccagt ggaaccacta accacaacgc ccagagcaaa
     4621 atcgtggtca cgttaccgcg cgtgctggcg atcaccgagg ccgcattctc actggaaccg
     4681 atcagcgcag cccactgctc cggggtgaga tgggctgtat agttcagata aatacggcta
     4741 ccgattactg ccagggcgta gatagcgatc ccggctttta cataccaaag caagttgaac
     4801 cgttgagcag acaacgccag gacagccagc aggcctccaa cccacatggc tgcgcctatc
     4861 cagggaaccg gcgcgcccat atcccattgg gcgatcagcc agactgtaaa cacgatgact
     4921 gtcatggtct gtgcagtgcg cggcagtcgg ttgattgatc cattgttctg gctgcgatca
     4981 ttgcggcgag ggcgaaagcc ggctcgctgc tggatttctg cgtcgggcag gagggcaagc
     5041 aggatcgcac acagcaggct gaccagtacg gctgaatttt ctgccaacca gtaagcaatg
     5101 accaggccgc cattcgccag gtaaaccggc aaatccatca aaaacatacc gatacgatcc
     5161 agctcgttca tggcgcgcca gccggcagta atgtcaccag ggttacatac acctgcatgg
     5221 tatcctgcac gaagagaacc ctgggttggg taatcggaat ggcaaccccg ttccagttga
     5281 aggccgtccc tgcggtataa acccacaaac gcaagtcaaa tgctccagga tcagcaaagg
     5341 gcacgttggg gacaaatgct tctgcagtgc aggtgccatt gccaccgcaa ccagcgttga
     5401 cggttgccat gccgggcagc aaatcgaacc ggtcctgatg gacataggct tcgtagtatt
     5461 tgctggcaag gtcgttcagg atccagtagc ggctctccgg gctaagctgg gcattcgcct
     5521 gaacttcggt gatcggctcg ggcaggattt ccacatgctc gatgcaatgg atctcaccat
     5581 cgacgacttc ataagccgga ttgtcctcca tggatggctc ccaattgttg tcatagcggc
     5641 ttccagggcc agggcagccc tcgccgttgc ctgagctggc agattcacag gtgggcggat
     5701 cttgaacttg ctcataccag gtgaagatga cggggggtat ggaggcagag gcctggatat
     5761 ccgcaccgcg cttttcgggg tcttggccaa cgaccagtgg attttcagga gcagccttct
     5821 ggatcgcaag cgttgctgga gcctgtgaga tggttggagc cgggcagata ggcgtctcgg
     5881 ggacacagct gccatccgag caatctgcca ccaatccggc gcagccacct ggcaggctgg
     5941 cgcagtccgc aacaccgttc aggtagagc
//
LOCUS       DATMXK010000152         3734 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_104674, whole genome shotgun sequence.
ACCESSION   DATMXK010000152 DATMXK010000000
VERSION     DATMXK010000152.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3734)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3734)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3734
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104674"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            54..1220
                     /locus_tag="VN363_02795"
     CDS             54..1220
                     /locus_tag="VN363_02795"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWQ83464.1"
                     /translation="MLKMSHRLRVATALNHEEPDAVPLDLGTGGNTSPVPEVYERLTH
                     LLQYPYELKPVPHIMRLATVDERILQYLDIDTRPIYMHPSQIGLRQCSEADHFYDEWG
                     VKWREYNLGGVIYRELAESPLQDATLDDLERYPWWPDPFDLTRYAGLETYAQKMFTQT
                     DYALVGCPAFNSLWERAYYLCGFQRMLEGLLTEPEFVHALFRKITDLIKQSLGIYLAR
                     VGKVIQVVKMGDDLGAQDNSLISPRIYRQTIQPYHRELFQFIHTQSPARVFLHSCGSV
                     YNLLPDLIDAGVDILNPVQVSAKNMDSPRLKNDFGDRLSFWGAIDTQHVLPHGSTGDV
                     TAEVQRRIADLAPGGGYILASVHNLQADVPAENIVTMYQTARIYGQYPLQAAAS"
     gene            1228..2355
                     /locus_tag="VN363_02800"
     CDS             1228..2355
                     /locus_tag="VN363_02800"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWQ83465.1"
                     /translation="MKTIAFPIGTALIGCGKVGVTHAQALAELPESRFMAVFDKSEDR
                     AQAFSAQYGVAAYTDLQAMLLRPDVQMATICTPNHTHPEMVEACAQARVHALVEKPMA
                     IDLQGCDRAIRAADTVGIRLGVISQRRLYRPVQRVKQAIMTGKIGQPVLGTLTVMGWR
                     DEDYYRMDAWRGRWATEGGGVLLTQTPHQLDLFQWFMGPIAEVFGYWDNYNHPYIEVE
                     DTAIAVVRFCNGGMGTVLVSNAQKPGFYGKIHVHGSNGASVGVQTDGGSPFVAGVTET
                     VEPPINDIWTVPGEEQYLEQWQAEDEDFCQTTNIMTHYHRLQIQDFLQAILEDRAVQI
                     DGREGRKHVELFTAIYRSQQQHQPVHFPLEAEADNAGLDGR"
     gene            complement(2386..3108)
                     /locus_tag="VN363_02805"
     CDS             complement(2386..3108)
                     /locus_tag="VN363_02805"
                     /inference="COORDINATES: protein motif:HMM:NF019349.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GntR family transcriptional regulator"
                     /protein_id="HWQ83466.1"
                     /translation="MFPNGNSDHLLTAPSQRTLSDYVADQLRQGIIIGALKPRQRLVE
                     EELAASMQTSRGPVRDALRLLEAEGLVVRQSHRGAFVAELTYEDVLEIYSIRELLESL
                     AIRFAIQKASSEQIDELERSVRTMSEMANQSYNQFLATDVDMEFHRTLCKISGHKRLL
                     ATWESLSAQIRLVVLTHRFHNPADLKERSVAWHEAIVDAIRRRDETKALAELHKHMSG
                     STEWVQRSIEIIEQQSGTNAGE"
     gene            3238..>3734
                     /locus_tag="VN363_02810"
     CDS             3238..>3734
                     /locus_tag="VN363_02810"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ83467.1"
                     /translation="MQNLFDMTGKVALITGAGANGGLGHTIALAFAEFGASIAAADID
                     AEGAQRTAQEVTAMGQKALAIHCDVSKPAEIEAMFAQVDNSFGRVDILVNIPFWFPRE
                     HPHELTLEGWNRTLEICLTSYFLCSQQAIRRMLEQGGGNILNIGSIAGASALGRGNFA
                     YSVAKA"
BASE COUNT          888 a         1021 c          973 g          852 t
ORIGIN      
        1 ttcctcagcc gtatccatat caactgtttc gtgaaatttg taggtgggtg cttatgctca
       61 agatgtcaca tcgcctgcgc gtggcaaccg ctctcaacca tgaagaaccg gatgctgtgc
      121 ccctagattt gggaacaggc ggcaatactt cacctgtccc ggaggtgtac gagcgcctca
      181 cccacctctt gcaatacccg tatgaattaa aaccagtccc gcacatcatg cgcctggcaa
      241 cagtggatga acgcatcctc cagtacctgg atattgacac ccgccctatc tatatgcatc
      301 ccagccagat tggcctgcgc caatgttcgg aagcagatca tttttacgat gaatggggcg
      361 tgaaatggcg tgaatataac ctgggaggag ttatctatcg cgagcttgcc gaaagccccc
      421 tgcaggatgc aacactggac gatctggagc gctacccctg gtggcctgat ccgtttgacc
      481 tcacgcgcta tgccggtctg gagacctacg cccagaaaat gttcacccag accgattatg
      541 ccctggtagg ctgcccggct ttcaacagcc tttgggaacg cgcctactat ctgtgcggct
      601 tccaaagaat gctggaaggt ttgctgaccg agccagaatt tgtgcatgcc ttgtttcgca
      661 agatcacaga cttaataaaa caatccctgg gcatatatct ggcccgggtg gggaaagtga
      721 tccaggtggt caaaatgggc gatgatctgg gcgcccagga caattcgctg atctcgccgc
      781 gcatttatcg ccagaccatc cagccctacc atcgcgagct gttccaattt atccacaccc
      841 aatctcctgc cagagtcttc ttgcatagtt gcggctcggt atacaacctg ctacccgatc
      901 tcatcgacgc cggggtagat atcttgaacc cggtccaggt aagcgccaag aacatggata
      961 gcccgcgtct caaaaacgat tttggcgacc ggctctcatt ttggggcgcc atcgacaccc
     1021 agcatgtgct cccgcacggc tcaactggtg acgtgaccgc agaagtgcag cgccgtatcg
     1081 ccgatctggc ccccggaggc gggtatatac tggcatcggt tcataacctg caggccgacg
     1141 tgccggctga gaatattgtc accatgtacc aaaccgcccg catctatggc caataccccc
     1201 tccaggctgc tgcaagctaa ggatcttatg aagaccatcg cttttcccat tggcactgcc
     1261 ttgatcggct gcggtaaggt gggcgtaacc catgcccagg cgctagcgga gctgcccgag
     1321 tcgcggttta tggccgtatt cgataagagt gaagatcgcg cccaggcttt ttctgcgcag
     1381 tatggggtgg cagcctacac cgatctacag gcaatgctcc tgcgaccaga tgtgcaaatg
     1441 gccactatct gcacacccaa ccacacccat cccgagatgg ttgaagcttg cgcccaggcg
     1501 agagtacatg ccctggtaga aaaacccatg gcgatcgacc tgcagggctg tgaccgggcg
     1561 attcgggctg ctgatacggt tggaatacgc ctgggtgtaa tcagccagcg tcgcttatac
     1621 cgccctgtac aaagggttaa gcaagccatc atgaccggca agatagggca acccgttttg
     1681 ggtaccctga ctgtgatggg ctggcgggat gaggactatt atcgcatgga tgcctggcgg
     1741 gggcgttggg cgactgaagg tggcggcgtt cttctcaccc agacccccca ccaattagac
     1801 ttatttcaat ggtttatggg accgattgct gaagtatttg gctattggga caattacaac
     1861 catccctaca tcgaagtcga agatacggcg attgctgtgg tacgcttctg caatggcgga
     1921 atgggcaccg ttctagtgag taatgcccag aaaccgggtt tctatggcaa gatacatgtc
     1981 catggcagca atggtgcgtc ggtaggcgta caaaccgatg ggggctcgcc attcgtagct
     2041 ggagtgaccg agaccgtcga acccccgatc aatgatatat ggaccgtgcc aggggaagaa
     2101 caatacctcg aacaatggca agcagaagac gaagactttt gccagacgac caatattatg
     2161 acccattacc atcgcctgca aatccaggat tttctgcagg caatcctgga ggatcgggcg
     2221 gtacagatcg acggacgcga aggccgcaag catgtggagc tatttactgc gatctatcgc
     2281 tcgcaacagc agcaccaacc agtccatttc cctctggagg cggaagctga caatgctggt
     2341 ttggatggac gctaagtttc agaacttgcg atcactcact ggcggtcatt cgccagcgtt
     2401 cgtccctgac tgttgttcaa ttatttcgat ggatctctgg acccactcag tggagccgga
     2461 catgtgttta tgcaattcgg ctaatgcttt ggtctcgtcc cgtcggcgaa tagcatcaac
     2521 aatggcttca tgccatgcaa ccgagcgttc ttttaaatcg gctggattgt ggaagcgatg
     2581 agtgagaacg accaggcgaa tttgagcact gagagattcc caggtcgcca gcaaacgctt
     2641 atgaccggag atcttgcaca aggtgcggtg aaattccata tccacatcag tggccaggaa
     2701 ctgattgtag gattggttgg ccatctcgct catcgtacgg accgaccgct ccagctcatc
     2761 aatctgctca gaggatgcct tttggatcgc gaaacggatc gctaatgatt ccaataattc
     2821 acgaatacta taaatttcca gcacatcttc ataagtcagc tcagccacaa aggctcctcg
     2881 gtgagattgg cgcactacca ggccctcggc ctccaataaa cgcaatgcat cccgcaccgg
     2941 gccgcggctg gtctgcatac tggctgccag ttcttcttca accagtctct ggcggggttt
     3001 gagcgcccca ataatgatcc cctgccgcag ttgatcggcg acataatcac tcagcgtcct
     3061 ttgggaggga gctgtaagca aatgatcact attaccattt ggaaacacaa tgacccatcc
     3121 ttcttgatat tgttcagcga ctacaattaa tcatataccc caaattactt cgttgtcaat
     3181 agttatcagt taacaaattg caaaagcacc aataaaacca gcctggagga aacctgaatg
     3241 caaaacctat ttgatatgac tgggaaagta gccctgatca ccggtgccgg agccaatggc
     3301 ggcctgggac ataccatcgc gttagcgttt gctgaatttg gggccagtat tgccgctgca
     3361 gatatcgatg ctgagggcgc ccaacgcacc gcccaggaag taaccgcaat gggacaaaaa
     3421 gcgctggcta tccactgtga tgtatccaaa cctgctgaaa ttgaagccat gtttgcccag
     3481 gtagacaatt cttttggccg ggtggatatc ctggtcaaca tcccgttctg gtttcctcgg
     3541 gagcatccgc acgaattaac cctggaaggt tggaaccgca ccctcgagat ctgcctgacc
     3601 agctattttc tgtgttcaca gcaagccatt cggcggatgc tggagcaagg cggtgggaat
     3661 atcctgaata ttggctctat tgccggagcc tcagccttgg gacggggaaa ctttgcctat
     3721 agtgtcgcca aagc
//
LOCUS       DATMXK010000153         3908 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_2751, whole genome shotgun sequence.
ACCESSION   DATMXK010000153 DATMXK010000000
VERSION     DATMXK010000153.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3908)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3908)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3908
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2751"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..938)
                     /locus_tag="VN363_02815"
     CDS             complement(<1..938)
                     /locus_tag="VN363_02815"
                     /inference="COORDINATES: protein motif:HMM:NF024842.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWQ83468.1"
                     /translation="MPEQHPRHSAYDAVIVGAGPNGLAAGIVLARAGLRVIILEGTSA
                     PGGGTRSAELTLPGFIHDPCAAVHPFGKASPFFRSLPLDEYGLKWIQSDAPLVHPLDG
                     GKVVVVERDIDATAAGLGSDGPAYRRWVKFWVEHWEELFADLVGPLPLPPKHPFTIAR
                     VAPQSLLPAETVARAFFQDAGTQVLFAGMAGHSMLPLNRPLTGAFGWMLSMLSHVIGW
                     PIVQGGTQGLANALVAYYRSLGGELVTDCWVKSMADLPPARAVLFDTPPRSVLNIAGD
                     ILPAAYRRQLGRFRYGQGVFKIDYALDAPIPWENPG"
     gene            complement(1015..1317)
                     /locus_tag="VN363_02820"
     CDS             complement(1015..1317)
                     /locus_tag="VN363_02820"
                     /inference="COORDINATES: protein motif:HMM:NF013868.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWQ83469.1"
                     /translation="MIKIKGEINSQAEAPLMAAYVEASQPGVQAIILDFSDLEYMNSS
                     GIGLLVTLLIRANRQRQRLMASGLSEHYKQIFDLTRLNEAIAIYDDEKSAVAAGSL"
     gene            complement(1446..1949)
                     /locus_tag="VN363_02825"
     CDS             complement(1446..1949)
                     /locus_tag="VN363_02825"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83470.1"
                     /translation="MMGDIGMDGIMPDDYLYRTRLDGMMDTSWRTLAEFDLPSEPGNE
                     KIAMARVASLIKPFKLPDAQVERIKTAVAEATMNAMEHGNHFQAEVPVQISLAASKTK
                     LQLQIEDHGKGPRRKDPEIPDLEAKLAGLQSPRGWGLYLIRSMVDELNVTTGDGFHRL
                     ELIFNLI"
     gene            2116..>3908
                     /locus_tag="VN363_02830"
     CDS             2116..>3908
                     /locus_tag="VN363_02830"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ83471.1"
                     /translation="MDKNPPFWALYLLGAPRLENAGQIVEMDTRKALALLAYLALAGG
                     QTRESLATLFWPEYDQASARGALRRTLSTLQKATLGDLLDISRERVQIRSSAPLRVDV
                     QVFHQLLLDANNPATAGSPGQSQQILQQAVDLVHGDFLQGFTLRDSPTFDDWQYFQTD
                     SLRRELGSALEKLALGYSQRTDDNEAIQAVRRWLALDELQEDAHRLLMKLYAWTGQRG
                     AALRQYRECVRILEQELGVPPLPETTQLYEAILENQLPARPAAETPAIPEYPANPTPG
                     EGYSLTGREAELGQLLNIYWTGAGNGAFVVVEGEAGIGKTRLAEEILGIAQRAGAATL
                     TARCYEGETQLAYGPFLEGIPNLLNQPGSQSALVKIPVHWLREAVRLVPALNELRPDL
                     PSPLPAEGPGAQSRFFEGIRQFLVGLLAGGQTGKLPGILLLDDLHWADPATLDLLIYL
                     VRRLRGHSLMVLVTLRDRAGAEAARISSLLAETIRSGYGYVIPLARLSPEAVALLSEH
                     SMQPLSEELRLRLYQETEGLPLLVLAYLDSMQPLDGTNWEMPASVRNIFHSRMPTPDS
                     PGWQLLTTAAVIGRAFDFDTLRQASGRSEVET"
BASE COUNT          877 a         1139 c         1029 g          863 t
ORIGIN      
        1 caaccagggt tttcccaggg aatcggggca tccagcgcgt aatcaatttt aaaaacaccc
       61 tgcccataac gaaaccgacc cagctggcga cgatatgccg ctggcagtat atctccagca
      121 atattcagca cactgcgtgg aggggtatcg aacaggactg cccgggctgg cggcaggtcc
      181 gccatggatt tcacccagca gtcggtcacc agctccccac ccagcgagcg ataataggct
      241 accagggcat ttgccagtcc ctgcgtaccg ccctggacaa tcggccagcc aatcacgtgc
      301 gacagcatgc tgagcatcca gccaaaagcg ccagttaagg gacggttcaa aggcagcatg
      361 gaatgcccgg ccatccccgc aaacaaaacc tgcgtgccgg cgtcctgaaa gaatgctcgc
      421 gccacggtct ctgccggtaa cagggattgc ggtgctaccc gggctatggt aaatggatgc
      481 ttcggtggga gcggaagcgg accgactaaa tcggcaaaca gctcttccca gtgctccacc
      541 caaaatttta cccagcgccg ataggcgggt ccatccgatc ccaggccagc tgctgtagca
      601 tcgatatcgc gctccactac gacaaccttc ccgccatcca gcggatggac gaggggagca
      661 tcagactgga tccatttcag cccatactca tccagcggca gggaacgaaa aaaaggcgat
      721 gccttcccaa agggatgcac cgccgcgcat ggatcgtgga taaagcccgg caaagtgagc
      781 tcggctgatc gggtgccacc accaggcgcc gaggtacctt ccagaataat gactcttaat
      841 cccgcccgcg ccaaaactat tccggccgcc aggccatttg gaccggcgcc tacaatgacc
      901 gcatcgtacg cgctgtgccg agggtgttgt tcaggcattc gtatgcatca gtatacttta
      961 attcctgccc gtttccaacc ggtaagctga ttagctggct gctgtgttta ctgattagag
     1021 gctgcccgca gcaacagcag atttttcatc atcatagatc gcaattgctt cgttcaggcg
     1081 ggtcaaatca aagatctgtt tatagtgctc gctgagaccg ctcgccatca atcgctggcg
     1141 ctggcgattt gccctgatca gcaaagtcac caataagccg atccccgagc tgttcatata
     1201 ttccaggtcg ctgaagtcaa ggataatcgc ctgtacccct ggttgggatg cttctacata
     1261 cgcagccatc aaaggagctt cagcttgaga attgatctca cccttgatct tgatcacacg
     1321 gtcagattat tcggagcctc tactgataac aaaactttcg gttcgttcat ggaattctcc
     1381 ttaagttgcc tgaactttat aaatcatcct gcactgatcg gcaaaattgc ctttcagcag
     1441 catgcctata tcaagttgaa aatcaactcc agacgatgaa aaccatcacc tgttgtgaca
     1501 ttcagctcgt ctaccatgct gcgaatgagg tacaaccccc atcctcgtgg ggattgtagc
     1561 cccgccagtt tggcttccag atctggaatt tcaggatctt ttcgcctggg acctttgcca
     1621 tgatcttcta tttgcaactg taattttgtt tttgaggcag ccaggctgat ttgcaccggg
     1681 acttcggcct ggaaatgatt gccatgctcc atagcattca tcgttgcctc cgccaccgca
     1741 gtcttaatcc gctcgacctg ggcatccggt aatttgaagg gttttatcag agatgcgacg
     1801 cgtgccatgg caattttttc attgcccggc tcactgggca aatcgaattc ggccagtgtg
     1861 cgccaggacg tgtccatcat accgtccaac ctggttctgt aaagatagtc atccggcatg
     1921 atgccatcca tacctatgtc gcccatcata agttcccacc tcgataataa tccactgctt
     1981 cacaccagga tagccaggca atcttccact ccaccttcat aattaagtgc aatgataatc
     2041 aatcagcgtc cagcaatcgt tacgaaaatc gttacgatat tttttcgtca cggtttgggg
     2101 taaaattggg ctgagatgga taaaaatccg ccattttggg ctttgtattt actgggtgcg
     2161 ccgcgcctgg aaaacgcggg acaaatcgtg gaaatggaca cgcgtaaagc gttggccctt
     2221 ctcgcttacc ttgcattggc tggaggtcaa acccgcgaat ccctggcaac cttgttttgg
     2281 ccagaatacg accaggcttc tgcccgcggc gccctgcggc ggacgctctc cacattgcag
     2341 aaagccaccc tgggcgacct gctcgatatc agccgcgagc gagttcagat ccgctccagc
     2401 gctccactcc gggttgatgt acaggtattt caccagctgc tcctggatgc caataacccg
     2461 gccacggcag gcagtcccgg ccaatctcaa cagatactcc aacaagccgt cgatctggtt
     2521 catggcgatt ttctccaggg tttcactttg cgcgatagcc cgactttcga cgactggcag
     2581 tactttcaga cggacagttt acgccgcgaa ttaggcagcg ccctggaaaa gctggcgttg
     2641 ggttacagcc agcgtacgga tgataacgaa gccatccaag cagtccggcg ctggctggca
     2701 ctggacgagc ttcaggagga cgcccaccgg ctattaatga aattatatgc ctggacaggg
     2761 cagcgagggg cggctttgcg ccagtaccgc gagtgtgtgc ggattctgga acaggagctg
     2821 ggtgtacctc ccttacctga aacaacccag ctttacgaag ctattctgga aaatcagctc
     2881 cccgccagac cggcagctga gacgccagcg atccccgagt accctgccaa cccaacgcct
     2941 ggcgaagggt attctctcac cggtcgagag gctgagcttg ggcagctatt gaatatctac
     3001 tggaccgggg ctggaaatgg cgcatttgtt gtggtcgaag gtgaggcagg gattggaaaa
     3061 actcgcctag ccgaagagat ccttggaatt gcccaacggg caggggcagc gaccctgacc
     3121 gcgcggtgtt acgaaggtga aacccagctc gcgtacgggc cctttttaga aggaatcccc
     3181 aatctgctca accagcccgg cagccagtcg gccctggtga aaattccagt tcattggctg
     3241 agggaggctg tgcgcctggt tcctgcattg aatgagctgc gccccgatct tccctcgcca
     3301 ttgccagcag agggacccgg cgcgcagagc cgtttttttg aaggcatccg ccagttcctg
     3361 gtagggctgc tcgcaggcgg ccagacaggc aaacttccag gtatcttatt gctggatgat
     3421 ttgcactggg ctgatccagc aacgttggat ttgctgattt atctcgtgcg ccgtctgcgc
     3481 ggccactcct taatggtact ggtcaccctg cgcgaccgcg ccggagcgga ggcggcacgc
     3541 atttcaagcc tcctggcaga aacgatccgc tcgggttatg gatatgtcat tcctctggcg
     3601 cgcttatcac cagaagcggt ggcactgctt tctgagcatt ccatgcagcc cctgtccgaa
     3661 gagctccgcc tgcggcttta tcaggaaacc gaaggcctgc ctctgctggt gctcgcctac
     3721 ctcgatagca tgcagccgct ggacggaaca aactgggaaa tgcctgccag tgtgcggaac
     3781 atattccaca gccggatgcc caccccggac agcccgggct ggcagctatt gacgactgcc
     3841 gcagtaatcg ggcgcgcctt tgattttgat accctccgcc aggccagcgg gcggagcgaa
     3901 gtggagac
//
LOCUS       DATMXK010000154         3894 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_17921, whole genome shotgun sequence.
ACCESSION   DATMXK010000154 DATMXK010000000
VERSION     DATMXK010000154.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3894)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3894)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3894
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17921"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..337)
                     /locus_tag="VN363_02835"
     CDS             complement(<1..337)
                     /locus_tag="VN363_02835"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ83472.1"
                     /translation="MPRWGMVIDLDKCFACQSCTAACRMENNTPVAGPDQTELGRAVL
                     WNEVLPFIEGEYPHVVATLIPRPCQHCDNPPCTKVCPVQATFINAEGIVEVNYQRCIG
                     CRMCTVGCPY"
     gene            complement(467..2887)
                     /locus_tag="VN363_02840"
     CDS             complement(467..2887)
                     /locus_tag="VN363_02840"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ83473.1"
                     /translation="MNNKPLKSNSESSGSGRRSFLKVAAAAGTVGAAALALKNVQIPA
                     LRTGLAAENGEEKIVKTACGLCPCGCGLDVRVVNGKAVKVEGNPLHPLNQGVCCLRAQ
                     ASLEVLYSPERLEHPRRQLGERGSGDWEEISWEEALDMIASRLGELRAAGQAHTVAFL
                     HGELRGQMRGLVGRFMQAYGSPNVISQDSLGEGLARLTMLLTQGVNAYPVYDLNNASY
                     VMAFGSNLLESSRHVIGYLGSCAFMRRGRPQRGKLVSVHPRMALTGVKADEWVPIRSG
                     TYAALALGMANVIINSGLYDAAYLKDFTYGFEDFEDMDGQTHKGFKSLVLEGYPLERA
                     ADITGVATDVIARLAGEFATNRPALAVMPTEPAELNAGNSLYTAMAIHALNALVGSLD
                     GPGGVLVQRFPDLGEWPAYSADVLGSESLAQPRLDGAGSTALPLAGSVYQDVARRILS
                     EDPYPVNALFLLNANPVYEVPNQGEVSQALLKVPFVVSFASTLDESAAHADLILPAST
                     FLEIWGDDFIEGTGFAGVTLRQPVVEPIHDTANPGDVLLQLAARLGGPVAEALPWSDY
                     QLLVRERLSALDLDWDKFLANGNWSEMVYFNAAPGSKAWEQVVGRDRLAAPRDGRFDF
                     FSRELYAILRNTRPELAEDPEQLDLACLPHFETPAALEGDLNQAVEFPFLLISQGLLT
                     HSRSWQGIIPTLQECYGLQTFVRWNSWLEINPATAAELGLADRDEVWVESPYGKVKAP
                     VRLYPGIYPNVVFMPQGQGHFTRVVWGHSSAAGQIGVNANTLMHNTTEPLTGQAIFNP
                     VRVKISKA"
     gene            complement(2895..3284)
                     /locus_tag="VN363_02845"
     CDS             complement(2895..3284)
                     /locus_tag="VN363_02845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83474.1"
                     /translation="MKMKPVTILILCLVLLGLALALPGRPAQALPEYSSQTGEPCATC
                     HASPSGGGVRTPRGQAWVAQSKPGEVPDLVAALDALGVSLDVNEADYVLVPDTIAPAA
                     ALSSSPAEASSSISREVQRWIAGYGGN"
     gene            complement(3296..3766)
                     /locus_tag="VN363_02850"
     CDS             complement(3296..3766)
                     /locus_tag="VN363_02850"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWQ83475.1"
                     /translation="MLTESEKTPSPTLAEKPSVFTSRRNFLQFGIAAVGAAWAGTLLQ
                     TRLFPIQTGPAEARPVEFPLSELPVGQTKLITYAGQPAIVLRTTESIKAFSLICTHLG
                     CTVEWQAANLEFYCPCHDGRFDQFGEVTAGPPPVPLEAYPVQIVDDKVVVGETA"
BASE COUNT          723 a         1178 c         1260 g          733 t
ORIGIN      
        1 cgtagggaca gcccaccgtg cacatgcggc agccgataca gcgctggtag ttcacttcga
       61 cgatgccctc ggcgttgatg aaggtcgcct gaaccgggca aacctttgtg cagggcgggt
      121 tgtcgcagtg ctggcagggg cgcgggataa gcgtggctac cacgtgcggg tactcacctt
      181 ctatgaacgg caggacctcg ttccacagga ccgcccggcc gagctcggtc tggtcaggtc
      241 cggccacggg ggtattattc tccatccggc aggctgccgt gcagctctgg caggcaaagc
      301 atttatccag gtctatcacc attccccagc ggggcattct gaacctccta cgtttttacc
      361 cagaccctac aggtttttat cggtttccag cctgcctgag ggggatttcc ctgacagtct
      421 ctcggctcga tcaggttctg cggaaacctg tagggtctaa tcgatactat gccttgctga
      481 tcttaacccg caccgggttg aagatcgcct gcccggtgag cggctcggtg gtattgtgca
      541 tcagggtgtt ggcgtttacc ccgatctgcc cggctgcact gctgtgcccc cagacgaccc
      601 gggtgaaatg cccctgaccc tgcggcataa agaccacatt ggggtagata cccgggtaca
      661 ggcgcacggg tgctttcact ttgccgtacg gcgattccac ccagacttca tcgcgatccg
      721 ccaggcccag ctctgcggcg gtggcggggt taatttccag ccacgaattc cagcgcacaa
      781 aggtctgcaa gccgtagcat tcctggaggg tggggatgat gccctgccag ctgcgcgagt
      841 gggtgagcag gccctggctg atgagcagga atgggaattc gaccgcctgg ttcaggtcgc
      901 cttccagggc agcaggagtc tcaaaatgcg gcaggcaggc gagatcgagc tgctctggat
      961 cttctgccag ctcagggcga gtgtttctca ggatggcata cagctcccgg gagaagaaat
     1021 cgaagcgccc gtccctgggg gcagcgaggc gatcgcgtcc caccacctgc tcccaggctt
     1081 tgctgcccgg ggcagcattg aaatagacca tctcggacca gttcccgttg gccaggaatt
     1141 tatcccagtc caggtccaga gccgaaaggc gctcgcgcac caggagctgg taatcggacc
     1201 agggtagagc ctcggctacc gggccgccca ggcgagccgc cagctggagg agcacatccc
     1261 ccgggttggc agtatcgtgg attggctcaa ccaccggctg gcgcagggtg actccggcaa
     1321 agccggtgcc ttcgatgaag tcgtcgcccc atatttcgag gaaggtgctg gcaggcagga
     1381 tcaggtcggc atgggcggca ctctcatcca gggtggaagc aaagctgacc acaaagggta
     1441 ctttgagcag cgcctggctg acttcgccct ggttgggcac ctcatagacc gggttggcgt
     1501 tgagcagaaa cagggcattg accggataag ggtcttcgga caggatgcgg cgggcgacat
     1561 cctgatacac cgagcctgcc aggggcaggg ctgttgagcc ggccccatcc aggcggggct
     1621 gggccaggct ctcgctaccc aggacgtcgg cggaatatgc aggccattcg cccaggtccg
     1681 ggaagcgctg caccagcacc ccgccggggc catccagtga gcccaccagg gcattcagcg
     1741 catgaattgc catggcggtg tacaggctgt tgccggcgtt cagctcagcc ggctcggtgg
     1801 gcatcactgc caatgccggg cggttggtgg cgaattcccc ggccaggcgg gcgatcacat
     1861 cggtggccac gccggtgata tcggcagccc gctccagtgg gtaaccttcc agaaccaggc
     1921 tcttgaagcc tttatgggtc tggccgtcca tatcttcgaa atcttcgaaa ccgtacgtga
     1981 aatctttcag atatgcggca tcatacaggc cggaattgat gatcacattc gccataccca
     2041 gcgccagggc ggcatacgta cctgagcgaa ttggcaccca ctcgtcggct ttgacgccgg
     2101 tgagcgccat gcgcgggtgt acggagacca gcttgccgcg ctgggggcgg ccgcggcgca
     2161 tgaaggcaca cgagcccagg tagccgatga cgtgccggct gctttcgagc aggttgctgc
     2221 cgaaagccat cacataactg gcattgttca ggtcataaac cgggtaagca ttcacgccct
     2281 gggtgaggag catggtcagg cgcgccaggc cttcgcccag gctgtcctgc gagatgacat
     2341 tgggcgagcc gtaggcctgc atgaagcgcc ctaccaggcc gcgcatctgg ccgcgcagct
     2401 cgccgtgcag gaaggccacg gtatgcgcct ggccggcggc gcgcagctcg cccaggcggc
     2461 tggcgatcat gtccagagct tcttcccagg agatctcctc ccaatcgccg ctgccgcgct
     2521 cgccgagctg gcggcgggga tgctccaggc gctcggggct gtacagcact tccagagagg
     2581 cctgggcgcg caggcagcac acaccctggt tgagcgggtg caggggattg ccttcgactt
     2641 tgacagcttt gccattgacc acgcgcacat ccaggccgca gccgcacggg cacaggccgc
     2701 aagcggtttt gacgatcttt tcctcgccgt tttcagctgc cagaccggtg cgcagggcag
     2761 ggatctggac atttttaagt gccagagcag cggctccaac cgttccggcg gcagcagcca
     2821 ccttgaggaa tgaacggcga ccggaacctg aagattcaga gtttgattta agcggcttat
     2881 tattcatcct ggccctaatt cccgccataa ccggcgatcc agcgctggac ttcgcgggag
     2941 atactggacg aagcctccgc cggcgagctc gacagggctg cggctggcgc aatggtgtct
     3001 gggacaagta cataatcagc ttcgttcaca tccagggaaa cccccagcgc gtccagggcg
     3061 gcgacgaggt cgggtacttc gccgggcttg ctctgggcga cccaggcctg accgcgcggc
     3121 gtgcgcaccc caccaccaga tgggctggcg tggcaggtcg cacagggctc gccggtctgc
     3181 gaggaatatt ctggcagcgc ctgggccggg cggcccggca gcgccagcgc caacccgagc
     3241 agaaccaggc acaggatcag gatcgtgaca ggcttcattt tcaccttgtt acctcctatg
     3301 ccgtctcacc aacgacgacc ttatcgtcca caatctggac gggataggct tccaacggga
     3361 ccggtggggg tccggcggtc acttcgccaa actggtcgaa gcgcccatca tggcaggggc
     3421 agtagaattc caggttggct gcctgccatt ccaccgtgca gcccaggtgg gtgcagatca
     3481 gagagaaggc tttaatgctc tcggtagtgc gcagcacgat ggcaggctgt ccggcatagg
     3541 tgatcagctt cgtctggccg acgggcagct cggaaagggg gaattccacc ggccgggctt
     3601 cggctggccc ggtctggatt gggaagaggc gagtttgcag cagggtgcct gcccaggccg
     3661 caccgacggc ggcaatccca aactggagga aattgcgccg ggaggtaaat accgagggtt
     3721 tttctgcaag agtaggggat ggtgtctttt ctgattcagt cagcatcgct gcacctcctt
     3781 acgggcgtgc gtccataccc aggatcatct gggcgccctg gcgcgtatcc tcaatgggat
     3841 tggcgccgac gtagttgtgg cagttattgc aggattcctc cggctggtgg cagt
//
LOCUS       DATMXK010000155         6801 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_105556, whole genome shotgun sequence.
ACCESSION   DATMXK010000155 DATMXK010000000
VERSION     DATMXK010000155.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6801)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6801)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6801
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105556"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VN363_02855"
     CDS             complement(<1..482)
                     /locus_tag="VN363_02855"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83476.1"
                     /translation="MWLLLLLGPLFISQRWLHREVQAIFLLLTRRTDVSIVLFALLFF
                     PGVLLHETSHYLVARLLGVRTGRFSLIPHPLPNGRLQLGFVETQASGWVRETLIGAAP
                     LISGGLLVAYAGVQRLGLTAAWDALSGGAPGAVQQALSAMFERPDFWLWFYLTIAVSS
                     T"
     gene            complement(550..1326)
                     /gene="tpiA"
                     /locus_tag="VN363_02860"
     CDS             complement(550..1326)
                     /gene="tpiA"
                     /locus_tag="VN363_02860"
                     /EC_number="5.3.1.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560327.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004807 - triose-phosphate isomerase
                     activity [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="triose-phosphate isomerase"
                     /protein_id="HWQ83477.1"
                     /translation="MRTPLVAGNWKMFKTVAEGRHLISELVPGLQAIKGVEKVLCPPF
                     TDLLAAAALLEGTDIGLGAQNMHWEASGAYTGEIAPPMVAELCQYVIIGHSERRAMFD
                     ETDETVNKKVGAALAHGLIPIVCVGETLEENETGRTAEVVARQIREGLAGMSLDVDNP
                     HASGRSLIIAYEPVWAIGTGKTATPEDANAVIAGHIRPALAEVLGDGIAQEIRVLYGG
                     SVKGSNAADFFIMPDIDGALVGGASLKASDFTQIVQAAAG"
     gene            complement(1447..2622)
                     /locus_tag="VN363_02865"
     CDS             complement(1447..2622)
                     /locus_tag="VN363_02865"
                     /EC_number="2.7.2.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004618 - phosphoglycerate kinase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate kinase"
                     /protein_id="HWQ83478.1"
                     /translation="MFNKKTIRDIDPRGKRVLVRVDFNVPIKDGVVTDDTRIQAALPT
                     INYLLENGAAVILFSHLGRPKDGPDPKFSMKPVAEYLAKLTGKPVAFAEEAAGPVAEA
                     AAAKLQPGEVLVLENTRFYPGETKNDPALSAQFAKLADLYVNDAFGSAHRAHSSTEGV
                     ARLLPAVAGFLMEKEIQYLGQAIAEPKRPFVAILGGAKISDKIGVIRNLLTKADQVLI
                     GGGMANTFFKAQGYPVGDSLVEDEVLDTARELVRSGSGKLRLPIDLVVADRFENEADA
                     KIMAMGPVEDGWRILDIGPETVKSFAKSIQNAGTVVWNGPMGVFEFPRFAQGTYGLAR
                     AVAESSATSIIGGGDSVAAINQSGLQDRITHISTGGGASLEMLEGLELPGLTALQDK"
     gene            complement(2692..3744)
                     /gene="gap"
                     /locus_tag="VN363_02870"
     CDS             complement(2692..3744)
                     /gene="gap"
                     /locus_tag="VN363_02870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007404202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA];
                     GO_process: GO:0019682 - glyceraldehyde-3-phosphate
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glyceraldehyde-3-phosphate dehydrogenase"
                     /protein_id="HWQ83479.1"
                     /translation="MAVKVGINGFGRIGRQVLKAIRDIHPDILEVVAFNDIGDLPTMA
                     HLLKYDSNYGRFDGTIEVKGEALLIDGKEVKAFKETDPANIPWGDLGVDIVVESTGIF
                     TIKDDGVNKKGKTVKGAVNHIKGGAKKVIISAPAQGEDLTIVLGVNDDKYDPQNHHVV
                     SNASCTTNGLAPAVKVANDKFGINRGFMVTVHSYTNDQKILDLPHSDLRRARAAAMNI
                     IPTSTGAARALKLVIPEMAGRLDGYSLRVPTSTVSVIDLTVELATPTTTEELRQAFRD
                     AAAGPLKGILAAVEEPLVSIDYKGDPHSSSVDLEFTQVLGKEANNFAKIVAWYDNEWG
                     YSVRTADLAAMMAKFL"
     gene            complement(3839..5176)
                     /locus_tag="VN363_02875"
     CDS             complement(3839..5176)
                     /locus_tag="VN363_02875"
                     /inference="COORDINATES: protein motif:HMM:TIGR01826.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconeogenesis factor YvcK family protein"
                     /protein_id="HWQ83480.1"
                     /translation="MTKPRSGWFHRLSGGLADMFRWLVPGLGIKRWYLFIFLGITLLA
                     IGFGVVILEVYRTTPDDAWWLKLISTASLRFLARPLRALIFGGLGVGMILFGIWKLNR
                     SMLSPFLTPGHSMLDTLRDHRQRGRGPRIVAIGGGHGQATLLRGLKQFSRNLTAVVSV
                     ADDGGSSGRLRRDLGIPPPGDIRNCLAALSNDEALLTQLFQYRFPAQYGSLEGHSFGN
                     LFISSLTEITGSFEDAVVESGRVLAIAGRVLPSSLNDVRLVADVKVPVTAREVRVEGE
                     SRIPDSPGKVMHVWLEPSNPPAFPEVIQAILAADMIVIGPGSLYTSVLPNLLVPDIMD
                     AVRASRGFKVFVCNVATQPGETDGYTVRDHLRALEAHVGGGFFEVVLANDRCDGRLPE
                     GVEWVPVDPDLNSDYPLQTADLNDDMRPARHDARKLAAILMELLEERTGPLVE"
     gene            5243..6250
                     /locus_tag="VN363_02880"
     CDS             5243..6250
                     /locus_tag="VN363_02880"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family protein"
                     /protein_id="HWQ83481.1"
                     /translation="MNEPDHRMSALCYTLSMSYHIAIDLGGTQMRAGLFPTGSLHPEK
                     LERIPTHSSGELPLTRLLDLIAAILPANDRVLAIAVAAPGPLDPHTGTIFTAPNVQGF
                     TNLPLRQIIVDRFGIPAAVGNDANLAALGEWKHGAGQGFHNLIYMTISTGIGAGVIVD
                     DHLLLGERGLATELGHVTVDPHGPICPCGKPGHLEAMAAGPAIARWTAAQIQAGEASS
                     LPPGQPITAKAVAQAALSGDPLAIRALERAGRLIGWALADYLHIFNPAAIILGGGVIQ
                     SGDLILGPLRQALKEYTLSNHYLEGLSLLTAALGDEAGLTGALALARQIAPEPADFSL
                     T"
     gene            6268..>6801
                     /locus_tag="VN363_02885"
     CDS             6268..>6801
                     /locus_tag="VN363_02885"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DHH family phosphoesterase"
                     /protein_id="HWQ83482.1"
                     /translation="MPTPTPKKRWQINPQITPIAAQNLVEYSAVLQQILFNRGHATAE
                     SARRYLEASPPPDSEPERMLGISESVERIRYALKHNEKIAIYGDYDVDGVTATALLTE
                     VLRGLGGSEIIPYIPNRFEEGYGLNREALELLKDNGVSLVITVDCGIRSLPEAEAARK
                     LGLDMIITDHHHPHTDLP"
BASE COUNT         1457 a         2023 c         1923 g         1398 t
ORIGIN      
        1 gtgctgctga ccgcaatcgt caggtagaac cacagccaga aatcgggccg ctcgaacatg
       61 gcgctcaagg cttgctggac agctcccgga gccccgccgc tcagggcgtc ccaggctgcg
      121 gtcaggccca ggcgctgcac accggcatag gctaccagca gacccccgct gatcaaaggt
      181 gcggccccga tcagggtctc acgcacccaa cccgaggcct gcgtctccac aaaacccagc
      241 tgcaggcgcc cgttgggcag tgggtggggg atcagcgaga agcgtccggt gcgcactccc
      301 agcagccgcg ccaccaggta gtggctggtt tcatggagca ggacaccggg gaagaacagg
      361 agagcgaaca ggacaatcga gacgtccgta cggcgagtga gcagcaagaa aatcgcctgg
      421 acttcacggt gcagccagcg ctgggatata aaaagtggcc ccagcaacag caggagccac
      481 attaagccac gtaattcttc cggcaccatc ctgaagcttt ctctggcgcg cagtgctcag
      541 cgagtaaagc tagcctgcag cagcctggac gatctgggta aagtcggagg ctttcaggct
      601 ggcgccgcct accagagccc cgtcgatatc gggcatgata aagaaatcgg cagcgttgct
      661 gcctttcacg gagccgccgt agaggacgcg gatctcctgg gcgatgccat cgcccagcac
      721 ttccgccagc gccgggcgga tatggccggc aatcactgca ttggcgtctt caggggtggc
      781 tgtttttccg gtgccaatcg cccagacggg ttcataggcg atgatcaacg agcgcccgct
      841 ggcgtgcggg ttatccacgt ccaggctcat cccggccagc ccttcccgga tctgcctggc
      901 gaccacctcg gcggtgcgtc cggtttcatt ctcttccagg gtctcgccca cgcacacgat
      961 cgggattaag ccatgcgcca gggctgcgcc gaccttttta ttgaccgtct catccgtctc
     1021 atcgaacatg gcccggcgtt cggaatgacc aataatgaca tactggcaca gctcggcaac
     1081 catcggcggg gcaatctcac cggtgtaagc gccggaggct tcccaatgca tattctgggc
     1141 gcccaggcca atatccgtgc cttcgagcag ggccgcggcc gccagcagat cggtgaaagg
     1201 tgggcaaagc accttctcca cgcctttgat ggcctgcagg ccaggcacca gctcggagat
     1261 caggtggcga ccttcggcca ccgttttgaa cattttccaa ttcccagcta ctaagggtgt
     1321 gcgcatgaac tgtcctcttt tttgaaaagg gatgctggct cgcaggcgct ggccagcatc
     1381 tcctcagatg tgctcgactg gctgcagcaa cgcgatcaat atgctcgcgg ctgcgcagat
     1441 ggatttttat ttatcctgta aggcggtcag gccgggcagc tccagacctt ccagcatctc
     1501 cagggaggcg ccgccgccgg tggagatatg ggtgatcctg tcctgcaggc cggattggtt
     1561 gatcgcagcc acggagtcac cgccgccgat gatcgaggtg gcactgctct cggctacggc
     1621 acgtgccaac ccataagtcc cctgggcaaa gcgagggaac tcaaacacac ccatcggtcc
     1681 attccagacg accgtgccgg cgttctggat ggatttggcg aaagatttga cggtctccgg
     1741 accgatatcc aggatccgcc agccgtcctc caccgggccc atcgccatga tttttgcgtc
     1801 ggcttcgttc tcgaagcggt cggcgaccac caaatcgatg ggcaggcgca gcttgccgct
     1861 gccgctccgg accagctcgc gggcagtatc gagaacttca tcttcgacca gcgagtcacc
     1921 cacggggtaa ccctgcgcct tgaagaaggt gttcgccatc ccaccaccga tcagcacctg
     1981 gtcagctttc gtgagcaggt tgcggatcac tccgatcttg tcactgattt tggccccgcc
     2041 caggatcgcc acaaaggggc gtttgggctc ggcaattgcc tggccgagat actggatctc
     2101 tttttccatc aggaagcccg ccactgccgg cagcagcctg gcaacacctt cggttgagga
     2161 atgggcgcgg tgggctgagc caaaagcgtc attgacatat agatcggcta atttcgcaaa
     2221 ttgagccgag agggccgggt cattcttggt ctcacccgga taaaagcggg tattttccag
     2281 gaccaggact tcaccgggct gcagttttgc cgcagcggct tcagccaccg ggccggctgc
     2341 ttcttcagca aacgcaactg gcttaccggt cagctttgcc aggtactcgg caaccggctt
     2401 catggaaaat ttggggtccg ggccatcttt ggggcgaccc agatgcgaga agaggattac
     2461 cgctgcgcca ttctccagca ggtagttgat ggtcggtaag gctgcctgga tgcgggtgtc
     2521 atccgtgacc acaccgtctt tgatgggcac attaaaatcc acccgcacca gaacgcgttt
     2581 gccgcgcggg tcgatatcgc gaattgtttt tttattgaac atgtggagcg cctccatgga
     2641 ggagagcgtg gaaaggaatt aactcctttc cacgctcgct gtaccgtttg gctagaggaa
     2701 tttcgccatc atggctgcca ggtcggcagt ccgcaccgaa taaccccatt cgttgtcgta
     2761 ccaggccacg atcttggcaa aattattcgc ttccttgccc agcacctggg tgaattccag
     2821 atccacagac gagctgtgcg gatcgccctt gtaatcgatc gagacaaggg gctcttccac
     2881 agcagccagg atgcccttga gcggtccggc tgcggcatcg cggaaagcct gacgcagctc
     2941 ttcggtggtg gtgggggttg ccagctcgac ggtcaggtcg atcacagata cggtggaggt
     3001 tggcacgcgc agagaatagc cgtccagtct gccagccatt tcggggatta ccagcttgag
     3061 tgcccgcgca gccccggtgg aggtggggat gatattcatc gccgcggcac gtgcccggcg
     3121 caggtcgctg tggggcaggt cgaggatctt ctggtcattg gtgtaagaat ggacagtaac
     3181 catgaagccg cgattaatcc caaatttgtc attggctacc ttcactgccg gggccagccc
     3241 gttggtggtg caggaggcat tagaaaccac gtggtgattc tggggatcat acttgtcatc
     3301 gttgacgccc agcacgatcg tgaggtcttc gccttgagca ggagccgaga tgatcacctt
     3361 tttagcgccg cctttaatat ggttgactgc gcctttgacc gttttacctt ttttatttac
     3421 gccatcgtct ttgattgtaa aaatgccggt gctctcgacg acgatatcaa cacccaggtc
     3481 accccagggg atattggcag gatcggtctc tttgaatgct ttgacttctt tgccgtcgat
     3541 cagcagagcc tcgcccttaa cttcgatcgt gccatcgaag cgaccgtagt tcgagtcgta
     3601 cttcaacaaa tgagccatgg tgggtaaatc accgatatca ttgaaggcca caacttcgag
     3661 gatgtcggga tgaatatccc ggattgcctt gaggacctgc cgtccaatcc gtccaaaacc
     3721 atttatgcct acttttactg ccatgatctt cctcctgata taaagatcct gattagatta
     3781 agctaccttc tggtggcttg aactgtcaac ctgtgagcag gtatccctgc ccaaattact
     3841 attctaccaa aggaccggtg cgctcttcaa gcaattccat caaaattgcg gccaatttgc
     3901 gcgcatcgtg gcgagccggg cgcatatcat catttaaatc ggctgtctgc agggggtagt
     3961 ccgaattcag atccggatcg accggcaccc actccacacc ttccggcaaa cgcccatcac
     4021 agcgatcatt ggcaagcacc acctcgaaaa atccgccgcc tacatgggct tccagggcac
     4081 gcaggtgatc ccggacagtg tagccatctg tttccccggg ctgcgtggca acattgcata
     4141 caaagacttt gaatccccgg ctggcgcgga cggcgtccat gatatccggg accagtaaat
     4201 tgggcagcac gctggtatac aggctgcctg gtccaattac gatcatatcc gcagccagga
     4261 ttgcctggat gacttctgga aatgccggcg ggttgctggg ctccagccag acatgcatga
     4321 ccttaccagg gctgtctggg atccggcttt caccttccac gcgtacttct ctggcggtta
     4381 caggcacttt tacatccgca accagacgta catcatttaa gctggatggt aaaactcgtc
     4441 cggcgattgc caggacacgt cccgattcga ccaccgcgtc ttcgaagctg ccggtgatct
     4501 cagtcagcga gctgataaac aggttgccaa atgaatgtcc ttccaggctg ccgtattggg
     4561 cgggaaagcg atattgaaac agctgagtga gcaaagcttc atcattggaa agtgcagcca
     4621 ggcagttgcg gatatcccct ggcgggggta tgccgagatc gcggcgcaga cggcctgacg
     4681 agccgccatc atccgctacg gaaaccaccg cggtgagatt gcgcgaaaac tgcttcaggc
     4741 cgcgcagcag ggttgcctgt ccgtgcccac cacctatcgc gacgatgcgg gggccgcgtc
     4801 cgcgctggcg atggtcacgc aaggtatcca gcatcgagtg cccgggggtg agaaaggggg
     4861 acagcatcga gcgattcagc ttccagatcc caaacaggat catgcccaca cccagcccac
     4921 cgaaaatgag ggcccttaac gggcgagcca gaaagcgcag ggaagcggta gaaatcagct
     4981 ttagccacca ggcgtcatct ggtgtagtcc ggtagacttc cagaattaca acgccaaacc
     5041 cgattgcaag cagggtaata cccaggaaaa taaacagata ccagcgcttg atccccaaac
     5101 cggggaccag ccagcggaac atatctgcca gtccccctga caggcggtgg aaccagcccg
     5161 aacgtggttt tgtcatacga tttgatcata gcagtcttta caggagtcgt caaccagaac
     5221 cttatcccca ggatacctgg cggtgaacga acctgaccac cggatgagtg ccttatgcta
     5281 tactctgagc atgagctatc atatcgcgat cgatctgggg ggaacccaaa tgcgggccgg
     5341 gctatttcca accggcagcc tgcaccccga aaaactggag cgcattccaa cccacagctc
     5401 aggtgagctg cccctgactc gcttactgga cttgatcgcc gcgatcttac cagcgaacga
     5461 ccgcgtgctg gcaatagcgg ttgccgctcc cggcccgctc gatccacata ccggtacaat
     5521 cttcactgcg ccaaacgtcc aggggttcac caacctccct ttgcgccaga tcattgtgga
     5581 tcgctttgga attccagctg ctgtgggcaa tgacgccaat ctggcagccc tgggtgaatg
     5641 gaaacacggc gctggtcagg gttttcacaa tctgatctat atgaccatca gcaccgggat
     5701 cggcgcgggc gtaatcgtag atgatcactt gctgctgggt gagcgcggcc tggcaaccga
     5761 attgggacat gttaccgttg atccccacgg accgatctgc ccgtgcggta aacccggaca
     5821 tctggaagct atggcggctg gtcctgcaat cgcccgctgg acggctgccc agatccaggc
     5881 aggtgaagcc agcagcctgc cacccggaca acccatcaca gcgaaagccg tagctcaggc
     5941 tgccctcagc ggtgatcccc tggcaatccg tgccctggag cgcgccggaa ggctgatagg
     6001 gtgggcgctg gcggattacc tgcacatatt taaccccgcc gcgatcatcc tgggcggcgg
     6061 ggtcattcag agcggtgacc tgattctggg tcccttacgg caggcgctca aagaatacac
     6121 tttatcgaac cattacctgg aaggattgag tttgcttacg gctgcccttg gagatgaagc
     6181 cggcctgacc ggcgcgctgg cgctggcgcg ccagatagct cccgaaccgg ctgatttctc
     6241 acttacgtaa gcccaatggt ttcatttatg cctacaccga caccgaaaaa acgctggcaa
     6301 attaacccac agatcacccc tatcgctgcc cagaatctgg tcgaatattc agccgtcttg
     6361 cagcagatcc tatttaaccg tgggcatgcc acagccgagt cagccagacg ctatctggaa
     6421 gcttctccgc cgcctgacag tgaaccggag cgtatgctgg gtatctccga atctgttgag
     6481 cgaattcgat atgctctgaa gcacaacgaa aagatcgcca tctatgggga ttatgatgtg
     6541 gacggcgtga ccgccaccgc gctactcacc gaagttctgc gcggcttagg tggcagcgaa
     6601 atcatcccgt acatccccaa ccgcttcgaa gaaggttatg ggttgaaccg ggaagctctg
     6661 gagctgctca aagataacgg agtcagcctg gtgatcacgg tagattgtgg gatccgctct
     6721 ctgcctgaag ccgaggcagc ccgcaagctt ggcctggata tgatcatcac cgatcaccac
     6781 cacccgcaca ccgatctacc a
//
LOCUS       DATMXK010000156         4684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_150760, whole genome shotgun sequence.
ACCESSION   DATMXK010000156 DATMXK010000000
VERSION     DATMXK010000156.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150760"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..204
                     /locus_tag="VN363_02890"
     CDS             <1..204
                     /locus_tag="VN363_02890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83483.1"
                     /translation="FQFGVRKSDDQAPPRGAALEVTAWIGQAFIAVAFGAIFAGVFLA
                     ALSALVGRLGFIVDFIFALLSAG"
     gene            211..1965
                     /locus_tag="VN363_02895"
     CDS             211..1965
                     /locus_tag="VN363_02895"
                     /inference="COORDINATES: protein motif:HMM:NF025309.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate mutase L"
                     /protein_id="HWQ83484.1"
                     /translation="MATSLIDADSLLVIDIGTVATRAILFDVVDGRYRFLAVGTGPTT
                     DRAPYRDVREGVRQAIDQLQLITSRALIGGNENLIIPSTPDGSGVDSCAVIMSTGTPL
                     KMVVVGLLEDVSLESARRLAATTYTGVVDTISLNDRRKTETRIDLILRLRPDMILVAG
                     GTEKGASQSILKLIEPVGLACYLMPEKQRPEILYVGNEALRTEVQSSLKSIVNLHFAP
                     NVRPDLEVEQLGSAQSELGKIYRRVRARQIIGVEELDKWAGGRLMPSATAFGRIVHFL
                     SRSYASSNGVLGVDIGANATTIAAAYEDDLHLGVYPQYGLGQGMNDLLKSIPLSQITR
                     WLTADTSEEYVRSYIQNKAIYPLTVPASAEDMGIEQALVRQLLQAAVKRSKPGFPDGL
                     QGSSNGLLPWFKIIVASGAALTKAPSLGQALLMLLDGLQPVGTATVGLDTNHLASALG
                     AAAEINPILTVQVLDSGTFQYLGSIISPVGGSPVGSPILRVKLQREGGDDSTLEVRQG
                     ALEVLPLSSGKTATLHLQPMHRYDVGMGGPGRGGRLTVHGGALGVIIDARGRPLQLPS
                     DAGRRRELYRKWLWTLGG"
     gene            2010..3077
                     /locus_tag="VN363_02900"
     CDS             2010..3077
                     /locus_tag="VN363_02900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83485.1"
                     /translation="MPARILAPITHILPLTTIQRERVLPVPGKIIVRKGQKVSASDTL
                     AEVDLNPEHMLLDLARGLGVSEDAVNETVQCRVGDLLAEGDIIAGPVGLARRVVRAPR
                     RGRVELIGGGQALLALQTSPYELKAGLSGQVVELISDRGAVVEAVGSLIQGVWGNGLI
                     DFGLMLVLAKSPDQILNASQLDVSMRGAVILGGHLEDAETLKTAADLPLRGLIIGSMD
                     PELIPLAQQVPFPLIVMDGFGHRRMTPAAFRLLSTNERREVSINAEPWNRDTGARPEV
                     VIPLPATGNPPVPRAALIFRVDQTVRVVKAPYAGMVGKINSLEPGLQVFPSGLRAKAA
                     EVSLENGENVLLPIANLEVID"
     gene            3103..3648
                     /locus_tag="VN363_02905"
     CDS             3103..3648
                     /locus_tag="VN363_02905"
                     /inference="COORDINATES: protein motif:HMM:TIGR01167.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009986 - cell surface [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LPXTG cell wall anchor domain-containing
                     protein"
                     /protein_id="HWQ83486.1"
                     /translation="MSFDDMDSNNDLEPRDTPPREESGNRTFLVAAGILGAITVLALL
                     FIALYALVLLPRQQATREQQLQDLNAQNTAVAMAITQTSAAMTAPTLTPTQPVANTAT
                     ATPTSVVVVATSTSSGPDPRTATVAALLTQAAVTTQTVVPTATALPNTGFADDVGLPA
                     MLGLAVLLIAVIFIARRLRTA"
     gene            complement(3757..>4684)
                     /locus_tag="VN363_02910"
     CDS             complement(3757..>4684)
                     /locus_tag="VN363_02910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561493.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HWQ83487.1"
                     /translation="HLRELINFDVIAENPQRLVVDSMYGSGRGVIKGILQGTGCEVQE
                     IRGEMNPGFGGVHPEPIAQHLGALAGAVSTHMGNFGLATDGDADRIGAMDERCNFVDP
                     HKIMALAMRYLIETRGCRGPVVRTVSTTRMIDRLAKKYDVPVYETPVGFNHIADYMVK
                     QDILIGGEESGGISFCGHIPEGDGVLMGLLIVEMVSSAGSTLYEMVEDLLAEVGPAYY
                     RRSDIRLSRPVAKQTMTEYMLNNAPREIGGEAINSISTLDGIKYLMADDSWLLIRPSG
                     TEPVLRVYAEGRTPQMVDALLGYGKHVAEEVT"
BASE COUNT         1060 a         1225 c         1272 g         1127 t
ORIGIN      
        1 ttccaattcg gtgtgcgcaa gtcggacgat caagctcctc cacgaggtgc ggctcttgaa
       61 gtaacagcct ggataggtca ggcattcatc gccgtcgcat ttggggctat atttgcaggt
      121 gtctttcttg cagcactcag cgcgctggta ggccgcctgg gatttattgt agatttcatc
      181 tttgccttat tgtccgcagg gtagacattt atggcgactt cattaatcga cgcagattct
      241 ctactggtca tagatattgg caccgttgcg acgcgcgcga ttttattcga tgttgtagac
      301 ggacgttatc gctttctggc agttgggacg ggaccaacaa cagatcgagc gccctatcgc
      361 gatgtgcgtg agggtgttcg ccaggcgatt gaccagctcc agctgatcac cagccgggcg
      421 ctgatcggtg gaaacgagaa cttaatcatt cccagcacac cggatggatc aggcgttgat
      481 tcttgtgcgg tcatcatgtc caccggtacg ccattaaaaa tggttgtggt aggccttctg
      541 gaagatgtat ccctggagag tgcacgccgg ctggcagcaa caacttatac aggtgttgtg
      601 gacacaatca gtttaaacga ccggcggaaa actgaaacgc gcatcgactt aattcttcgt
      661 ttacgcccgg acatgatcct ggtcgccggg ggaacagaaa agggcgccag ccaatctatc
      721 ctgaaattga tcgagcctgt tgggctagcc tgttacttga tgccagaaaa gcagcgtcct
      781 gaaattctct atgtgggcaa tgaagctctc cgaacagagg tacagagctc gctgaagagt
      841 atcgtcaacc tgcattttgc cccaaatgtc cggcctgatc ttgaagtgga acagctgggt
      901 tcagctcaga gcgagctggg aaaaatttac cggcgggtca gagcacggca aattatcggg
      961 gttgaagagt tagacaaatg ggctggtggg cgattaatgc catctgctac agcttttggg
     1021 cggatcgttc acttcctgag ccgctcctac gcatcttcga atggtgtttt gggagtcgat
     1081 atcggtgcca acgcgacaac catcgccgcg gcatacgagg atgatctcca tctgggagtg
     1141 tacccccagt atggtttggg gcaagggatg aatgatttgt tgaagtccat cccactcagt
     1201 cagatcaccc gctggctgac ggcagacacc tctgaagaat atgtgcgcag ctatattcag
     1261 aataaagcca tttatccact gactgtgccc gccagtgcgg aagatatggg gattgagcaa
     1321 gcccttgtac gccaattatt acaggcggcg gtaaaacgct ctaaaccagg cttcccggac
     1381 ggtttacagg gctcttcaaa tggattattg ccatggttca aaattatcgt tgcctctggc
     1441 gctgcgctga ccaaagcgcc ttcgttaggt caggcattat tgatgcttct ggatgggctt
     1501 cagccggtgg gtacagcaac tgtagggctt gacaccaatc atcttgcatc tgctttaggt
     1561 gctgcggctg aaattaatcc aatcctgacg gtacaggttc tggattcagg gactttccag
     1621 tatctgggat cgatcatttc cccggtcgga ggctcccctg tcggctcacc catcctgagg
     1681 gttaagttgc agcgtgaagg tggagatgac tcaacccttg aagtcagaca gggcgcactt
     1741 gaggtactac ctctatcaag cggaaaaact gccacgctgc atttacagcc gatgcaccgc
     1801 tacgacgtcg gcatgggcgg ccctggccgt ggtgggcggc tgacggttca tggaggtgct
     1861 cttggcgtga ttatcgacgc tcgtggaagg cctttgcagc ttccttcgga tgctggcagg
     1921 cggcgagagc tgtatcgaaa atggttatgg acactcggtg gctgatcggg cactgaactt
     1981 gcatacaaca gattaaggaa ttaacatcca tgccggcacg tatcctggct cccatcaccc
     2041 atatattacc gctcacgacc atccagcgcg agcgtgtcct gcctgtgcct gggaaaatta
     2101 ttgtccgcaa aggccagaaa gtgtccgcaa gcgatacact tgcagaagtt gacctgaacc
     2161 ctgagcacat gttactcgat ctcgcccgcg gtctgggtgt ctcggaagat gctgtgaatg
     2221 agactgtgca gtgccgggta ggtgatctgt tagctgaagg agatattatt gccgggccgg
     2281 ttggcctggc acggcgggta gtacgggcac ccaggcgagg ccgggtggag ttgatcggtg
     2341 gcggtcaggc gctcctggct ttacaaactt cgccttatga gctgaaggct ggtttatcgg
     2401 gtcaggtggt tgaattgatt tctgaccgtg gagcagttgt tgaagccgtt ggctcactga
     2461 ttcagggagt ctgggggaat ggcttgatcg atttcggtct gatgctcgtc ctggctaaaa
     2521 gcccggatca aattctgaat gcatcccagc tcgatgtcag catgcgcggt gcagtgatat
     2581 tgggaggtca tctggaagat gcagagacct tgaaaactgc tgcggattta cctttgcgtg
     2641 gattgattat cggaagtatg gacccggagc tgataccttt agcccagcag gtcccttttc
     2701 ctttgatcgt tatggatggt ttcggacacc ggcggatgac ccctgcagct tttcgattac
     2761 tttcgacgaa tgaacgccgc gaagtttcga taaatgcaga gccatggaac cgggacacgg
     2821 gggcacgacc tgaagttgtt attccgctgc cggctacagg aaatcccccc gttccgcgtg
     2881 ccgccttgat cttcagagtt gaccagaccg tgcgggtcgt aaaagcgcct tatgccggga
     2941 tggtgggtaa gattaacagc ctggagccgg gattgcaggt gttccccagt ggcttacgtg
     3001 caaaagcagc cgaggtcagt ctggaaaatg gcgagaatgt gctgttaccg atcgcaaatc
     3061 ttgaagtgat cgattaatcc tttcccctac aggaggagaa acatgtcttt cgatgatatg
     3121 gattcaaaca atgatttaga acctcgtgat actccaccaa gagaggagtc aggaaaccgc
     3181 accttcctgg tagcagctgg cattttgggc gcaataactg ttttagcgct attattcatt
     3241 gccctgtatg ccctggtact gctccctcgc cagcaggcaa cccgtgaaca acaactgcag
     3301 gatctcaatg cacagaatac cgcagtggct atggcaatca cgcagacatc tgctgccatg
     3361 actgcgccaa ccctgacccc gacccagcct gttgccaata ccgccacggc aacgccaacg
     3421 tcggtggtgg tagttgctac cagcacctca tccggacctg accccaggac agcaacagtc
     3481 gccgcgttac tgactcaggc agctgtaaca acccagacgg tagtgccaac tgccacggcc
     3541 ctgccaaata ccggatttgc tgatgatgtt ggcctacctg cgatgcttgg gttggcagtc
     3601 ctgctgattg cagtaatatt tatcgcccgc cgcttgcgca cggcataggt attttattct
     3661 taatcaaatc ggcggacgaa cctgggtggc tgtctcaaac gacagtgatc cgggttcttc
     3721 cgccttcttt tattccagag aacgccgcgg ttcttttcag gtaacttcct cagcaacgtg
     3781 cttgccatac ccaagcagtg catctaccat ctggggagtg cgtccttccg catagactcg
     3841 cagcaccggc tcagtacccg atggccggat taatagccag gaatcatccg ccatcaggta
     3901 tttaatcccg tccagagtgc tgatcgaatt gatagcttca ccaccgattt ccctgggggc
     3961 gttattgagc atatattctg tcatggtctg cttggcgacc ggacggctca ggcgtatatc
     4021 actccggcgg taataagcag gtccaacttc agcgagaaga tcttcaacca tttcatacaa
     4081 ggttgacccg gcgctggaca ccatctcaac gatcaaaaga cccattaaga ctccgtctcc
     4141 ttctgggata tgcccgcaga aggaaattcc acccgattct tcacccccaa tcaggatgtc
     4201 ctgtttcacc atataatctg cgatgtggtt gaatcccacc ggggtctcat agactggaac
     4261 atcatatttt tttgccaggc ggtcaatcat ccgggtggtc gaaacagtcc gcacgacagg
     4321 tccccggcag ccacgggttt cgatgaggta gcgcatggca agggccataa tcttgtgggg
     4381 atctacaaaa ttacaccgct catccattgc ccctatccgg tcagcgtcgc catccgtcgc
     4441 caggccaaaa ttacccatat gggtgctgac agcgccagca agggcaccca ggtgctgggc
     4501 aattggttca ggatggaccc caccaaagcc cgggttcatc tcaccgcgaa tttcctgaac
     4561 ttcacaccct gtgccctgaa ggatgccttt gataacccct cgccctgagc catacataga
     4621 gtcaaccaca aggcgttggg gattttcggc aatgacatcg aaattaatca actcacgtaa
     4681 atgc
//
LOCUS       DATMXK010000157         5413 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_69488, whole genome shotgun sequence.
ACCESSION   DATMXK010000157 DATMXK010000000
VERSION     DATMXK010000157.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5413)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5413)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5413
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69488"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>5413)
                     /locus_tag="VN363_02915"
     CDS             complement(<1..>5413)
                     /locus_tag="VN363_02915"
                     /inference="COORDINATES: protein motif:HMM:NF017930.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glucoamylase family protein"
                     /protein_id="HWQ83488.1"
                     /translation="FETRDRYRKAVEEIAKFGRLDEMEVARSAESLARAAEAQFTRSP
                     DAFKGDSSNQPGDQSAGWDRFRSKLESHIGHYLLGQGRSALERAAGYTPRGRLSLERA
                     TRSHPTRFYLGSIAILSVLLLLVPLLYAANHEALLSELILVFILALIPTLTVVVEVVN
                     WIFTQVIKPQSLPSMDFRNGLPEECTTLVVIPAMLSGPDEIDSLIAQLEQHYLRNLDT
                     GLFFALVTDYLDSATENQPGDSNLVKRAKEGIRSLNDKYPLRSSAHVTADRFALLHRE
                     RQWNPSEGIWMGWERKRGKLHELNQLILSAREILQTGQTLKSPSSFPFYEGDLGMLSR
                     VRYVITLDADTILPRDAARELIAILAHPLNRAHLNAPRPGECGAKVSSGYTILQPRTD
                     INPLSSGLSYFTRVFSGDTGLDLYTRAISDIYQDLFGEGSYVGKGAYEVESFERSLEG
                     CIPENALLSHDLLEGIHGRTALVTSTILIEDIPPNLLVHSRRQHRWTRGDWQLLPWLI
                     SSRLTRISRWKILDNLRRSLVPPALLLFFLAGWLDLSGSPGVWTVMGALALSMPVLTG
                     IIGTLRQKLSGKSARQSASSLVNSLLRWLLAMAFLPYEAYTVTDAILKTLVRMGITRR
                     GLLRWTTSDQVARRLGDQGALAIWAQMAISPILAIGTGILLAMLRPGALLWAAPFLTG
                     WLLAPGLAIWLSRPPRRQVDKLNNSQMRELRRLARQTWLFFEQFIGPEDQWLPPDHFQ
                     EVPLGIVAHRTSPTNIGMALLSTLGAYDLGYLEPLTLSTRLVSTLDTLEKLERYRGHF
                     LNWYDTRSLDPLQPRYVSTVDSGNLAASLLALRQGCLEIPDNPVLRWESFEGLIDAID
                     LLDSVFMDLDSDSLSVRPAVREVRSTLNEIVKAIQSVKELPSQWLKLLSDLGINGKGE
                     ITGRGWSEIDGLIVELVEKHALELGPQNLGRLRYYNRGARQQINNIQRSIDLLLPWLF
                     YFQEIPAVFQENDLPADVANAWNVLEGAFHTQPSLAEIPVFKNTVREALKELKVALEN
                     LTGAEWMDARRWIQGLNDTLEPEGATAELLLIGFSELARRSDAIFQAMDFSFLFDSQR
                     QVFHIGYNVTTSSLDLNYYDLLASEARLASLVAIAKGEVPQSHWLYLSRPITFINNRQ
                     ALVSWSATMFEYLMPGLMVSSLPSTLLGQTLEVVADRQIEYGRDNNIPWGISESGYYR
                     FDANQFYQYRAFGVPGLGYKRGASDDLVITPYASLLALPLRPLAVLQNITRLKQKGTL
                     GLYGFYEAIDFTPERLPAGQEKAIIQSYMAHHQGMIMLSLVNYLRGDMIVRRFQRDPR
                     IQSVSLLLMEQVPKDAPVEQLNTQNSPIMHVTRTSNPIIPWQVDPNAPQPRVHTLSNG
                     RYRLMITAAGGGYSSWEDIDLTRWRADSTLDNWGTWIYIQDLENDATWSSSLQPTLNA
                     GQEQKVLFSNQFAEFHRREGSLSQVTEIFIAPEDDLEVRLVTITNHGEKPRRLRLTSY
                     GELVLTHQIDDSRHPAFNKLFIESEHLPRGNGILFHRRLRSKSEKDVYLAHALVTTTG
                     ESTVVRPRLETDRRRFLGRGRTARYPAALETGSHPGTEPSLDPIFSISQVIFFPPHST
                     LRFAFLTATARSAEASQAIIDRYSSLGAVQRAMAQARGLAEVELSELEISSDQLKDFQ
                     TLLSGLVFPSAALRASSHVLAANRKDQTGLWPFAISGDYPILLARFESEHELNLAHQL
                     LQAHAYWHKQQLMVDLVFLDMQGTTYNQELSAQLYRQIASSGSEEWLNRRGGIFVLHA
                     DQIEEADRTLLATVARVVLD"
BASE COUNT         1289 a         1414 c         1480 g         1230 t
ORIGIN      
        1 atcgagcact acccttgcga cggttgccaa aagggtccgg tcagcttctt ctatctggtc
       61 tgcatggaga acgaagatac caccccggcg gttgagccat tcttcacttc cgctgcttgc
      121 gatctggcga taaagctgcg cactgagttc ctggttataa gtggtgcctt gcatatccag
      181 aaataccagg tcgaccatga gctgctgctt atgccagtaa gcgtgcgcct gaagcaactg
      241 gtgggccagg ttgagctcat gctcgctttc aaacctcgca agcaggatgg ggtagtcccc
      301 agatatcgcg aatggccaca atcccgtctg gtcttttcga ttagcagcca gcacgtggga
      361 gctggcacgc aatgccgccg atgggaagac taaccccgac aacaaggttt gaaaatcctt
      421 cagttgatcg ctggaaatct cgagctcact aagctctact tcagcaagcc cgcgagcctg
      481 tgccatggct cgctggaccg cgccaagaga agagtagcga tcgattatag cctggctggc
      541 ctccgccgag cgggctgtag ctgtcaaaaa tgcgaaacgc agtgtgctat gaggcggaaa
      601 aaaaattacc tggctgatcg aaaaaatggg atctaaagat ggctcagttc caggatgtga
      661 tccagtctcc agggcagccg gataacgggc agtacgacct cgccccagga agcgacggcg
      721 gtctgtctct aatctcggcc ggactactgt agattctcct gtggtggtga ccagcgcatg
      781 ggcgagataa acgtctttct cagacttgga gcgcagccgg cggtggaaca atatcccatt
      841 tcccctgggc aggtgctcgc tttcaatgaa aagcttattg aaagccggat ggcgtgagtc
      901 atctatttgg tgagtaagga cgagttctcc ataactggtc aggcgtaaac gccgtggttt
      961 ttctccgtga ttggttatcg tcaccaggcg aacctcgagg tcgtcttccg gagcaataaa
     1021 gatttcggtt acctggctta aggaaccttc ccggcggtgg aactcggcaa attgattgct
     1081 gaataagacc ttttgctcct ggccagcgtt taaggttggt tgaaggctgg aggaccaggt
     1141 tgcgtcgttt tccaggtcct ggatataaat ccatgtcccc caattatcca gggtgctgtc
     1201 agcccgccaa cgcgtcagat caatatcctc ccaggagcta tatcccccgc ccgctgcagt
     1261 gatcattaga cgataacgac catttgagag ggtatggact ctgggctggg gagcgttagg
     1321 atccacctgc caggggatga ttggattgct ggtccgcgtc acatgcatga tcggtgaatt
     1381 ctgtgtgttt agttgctcga ccggtgcatc ttttggaact tgttccatta ataacagcga
     1441 aacactctgg atgcggggat cacgctggaa acgccggaca atcatatctc cgcgcaggta
     1501 attgaccaga gacagcatga tcattccctg atggtgcgcc atataagact ggatgatcgc
     1561 tttctcctgg cctgcaggca atcgctcagg ggtgaagtcg atagcctcat aaaacccata
     1621 caaacccagc gtgccctttt gttttaagcg ggtaatattt tgaagaaccg ccaacggacg
     1681 cagcgggagg gcaagcaaag atgcataagg tgtgatgaca aggtcgtcgg aggcaccccg
     1741 tttataaccc aaaccaggca ccccaaaagc gcgatattgg tagaattggt tggcgtcaaa
     1801 gcggtaatag ccggattcgg aaatgcccca tgggatgttg ttatcacgcc catactcaat
     1861 ctgccggtca gcaaccactt ccagagtttg ccccagcagt gtgctgggta aactggacac
     1921 catcaggccg ggcattagat actcgaacat cgtcgcgctc caggatacga gcgcttgccg
     1981 gttattaata aacgtaatgg ggcggctgag atagagccaa tgactctggg ggacctcgcc
     2041 tttggcgatg gctaccaggc ttgccaaacg tgcttccgag gccagtaaat cgtaataatt
     2101 taaatccagg ctgctggtag taacgttgta gccgatatgg aaaacctggc gttgagaatc
     2161 gaacaggaag gaaaaatcca tcgcctggaa aatagcatcg ctgcgcctcg ccagctcact
     2221 aaaacctatc aaaagcagct cagctgtcgc tccttcaggt tccaacgtgt cattcagacc
     2281 ctggatccag cggcgtgcat ccatccattc tgctccagtc agattttcca gcgcaacttt
     2341 gagctctttc aacgcctcac ggacagtatt cttgaaaacg ggaatctctg ccagagaggg
     2401 ttgggtgtga aatgctcctt ccaacacatt ccaggcgttg gctacatcgg caggtaaatc
     2461 attttcctgg aagacagcag gaatctcttg gaaataaaat aaccagggta aaagtaagtc
     2521 aatactccgt tggatattat tgatctgctg gcgcgcgcca cggttgtaat acctaagccg
     2581 gcccagattt tgaggtccga gctccagggc atgcttttca accagctcca cgataagccc
     2641 atcaatttcg gaccaacccc gcccagtaat ctcccctttt ccattaattc ccagatcaga
     2701 gagcagcttt aaccactggg aaggcagctc ttttactgat tggattgctt taacgatttc
     2761 attaagcgtg cttcttacct ctcggacagc tggccgtacg gagaggctat cagagtccag
     2821 atccataaaa acagaatcca gtaagtcgat cgcgtcaatc agaccttcaa atgattccca
     2881 gcgtaaaact gggttatcgg ggatttccag gcatccctgg cgcagggcga ggaggctggc
     2941 tgccagatta ccactatcaa ccgtggatac atagcggggt tgtaatggat ccaggctgcg
     3001 tgtgtcgtac caatttaaaa agtgcccccg ataacgctcc agcttttcaa gagtatccag
     3061 ggtggaaacc aggcgtgttg agagagtcaa tggctcaagg tagcccaggt cgtaagctcc
     3121 cagggtcgat agtagtgcca taccgatatt ggttggcgat gttcggtggg cgacaatccc
     3181 cagagggact tcttgaaaat ggtcgggggg cagccactga tcctccggtc cgatgaattg
     3241 ttcgaagaaa agccaggtct ggcgtgccaa ccggcgcagt tcgcgcatct ggctgttgtt
     3301 gagcttatct acctgtctcc tcggaggccg gctgagccag atagctaatc ccggagccag
     3361 cagccagcct gtcaggaatg gagccgccca gagcagggct ccggggcgaa gcatagccag
     3421 caggatacct gttccgattg ccaggattgg agagattgcc atctgcgccc agatcgccag
     3481 cgcgccttga tcgcccaggc ggcgggcaac ctggtcactg gtcgtccagc gcagtaatcc
     3541 tcggcgggtg atacccatcc gcacaagggt cttaaggatc gcgtctgtaa ctgtgtaggc
     3601 ctcataaggc aggaaagcca tggccagcag ccagcgaagg aggctgttaa ccagagacga
     3661 tgcactctga cgagcagatt tgccgctcag cttctggcgt agcgttccaa taattccggt
     3721 taaaaccggc atggagaggg caagcgctcc catgactgtc cagactcccg ggctgccaga
     3781 caggtccagc cagccagcca ggaaaaaaag cagcagtgca ggaggcacta aactccgccg
     3841 tagattatcc aggatcttcc aacggctgat cctggtcagg cggctggaga ttaaccacgg
     3901 tagcagctgc caatcacccc gtgtccagcg gtgctgccgg cgggaatgta ccagcaggtt
     3961 cgggggtata tcttcgatca ggatggtgct ggtcacgagc gctgtccggc catgaatgcc
     4021 ttcgagcaag tcgtggctca ggagggcgtt ttcggggatg catccctcca ggctgcgctc
     4081 gaaactctcg acctcatacg cacccttgcc gacataactg ccttctccaa agaggtcctg
     4141 gtagatatcc gaaattgcgc gcgtgtagag atcaaggcca gtatcacctg agaacactcg
     4201 cgtgaagtag gataagccgc tgcttaatgg attgatatcg gtccgtggct ggagaatggt
     4261 atagcctgag ctgacttttg caccgcattc acccggtctg ggggcattca ggtgggcgcg
     4321 gttcaaggga tgggccagga tggcaattag ctcgcgcgca gcatctcgtg ggaggatcgt
     4381 atcagcatcc agtgtgatta cataacgcac gcgtgacagc atccccaaat ctccttcgta
     4441 gaaggggaag gaggatggcg atttaagagt ctgcccggtt tggaggattt ctcttgctga
     4501 cagaatcaac tggttaagct cgtggagttt gccgcgcttg cgctcccagc ccatccaaat
     4561 accttctgag ggattccact gccgctcccg atggagtaag gcgaagcgat ctgcggtgac
     4621 atgcgcagaa cttctcaatg ggtatttatc attcaggctg cgaatcccct cttttgccct
     4681 cttgacaagg tttgaatcgc cgggttgatt ttcagttgca gaatcgaggt aatcggtaac
     4741 cagggcaaag aaaagccctg tatcgaggtt gcgtaagtag tgctgctcca gttgcgcgat
     4801 cagcgaatcg atttcatccg gtccagaaag catagccggg atgacgacta aagtggtaca
     4861 ttcctcgggt aagccgttcc tgaaatccat acttgggaga ctctgaggtt tgataacctg
     4921 cgtgaagatc caatttacca cctctacaac gactgtcagc gtggggatca gggcaagaat
     4981 gaaaaccaat ataagttcag ataggagagc ttcatggttg gcagcataga gtaatgggac
     5041 cagtaacaat aataccgata ggatagctat cgatcccagg taaaatcgag tgggatgact
     5101 gcgagtggcg cgctctaaac ttagccgacc tcgtggggta tatccagcag ctctttccag
     5161 tgcagatcga ccttgaccca acaggtaatg cccgatatga ctttcaagct ttgatcggaa
     5221 gcggtcccag ccagcgctct ggtctcccgg ttgattactg ctatctcctt taaaggcatc
     5281 cggagagcgg gtaaattgtg cctctgccgc cctggccagg ctctccgcgc tgcgcgctac
     5341 ctccatctcg tccaaccgtc caaatttcgc gatttcctct actgctttcc ggtaacgatc
     5401 gcgagtctca aag
//
LOCUS       DATMXK010000158         4189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_40544, whole genome shotgun sequence.
ACCESSION   DATMXK010000158 DATMXK010000000
VERSION     DATMXK010000158.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40544"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..333
                     /locus_tag="VN363_02920"
     CDS             <1..333
                     /locus_tag="VN363_02920"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWQ83489.1"
                     /translation="AEALSVYGCELIVIKRGENGQYLYDAATRSCWEIPPYPARMVDP
                     TGAGDALCGGFLAGYRRTYDPLEAVLHGNISASLVIEGSKPNYALDALPGLAEARLQA
                     LRQAVRKL"
     gene            330..1466
                     /locus_tag="VN363_02925"
     CDS             330..1466
                     /locus_tag="VN363_02925"
                     /inference="COORDINATES: protein motif:HMM:NF013696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M20/M25/M40 family metallo-hydrolase"
                     /protein_id="HWQ83490.1"
                     /translation="MSLLIPDLPERVLQEAITIQQIPAPTHAETARSRYVFQRFVEEG
                     LADVQMDSPGNVYGCLPGSGTGLPVVISAHLDTVFPADTDLTLRPEQDRLYGPGIGDN
                     SVGVAGLFGIVWELRARHGDPGGPATRLPGDVWLVANVCEEGLGNLTGMRAVVERFGA
                     EVAAYLVLEGMSLGQIYHRGLGVKRYRIRVQTAGGHSWVDYGQPSAVNELAQLICELT
                     RLPLPARPRTTLNVGVITGGTTVNTIAARAEMELDLRSEDPQVLQELASQVETLIQRA
                     NRPDVKVTGEVIGDRPVGKISARHPLVQLAKTCLSELGIEANLGIGSTDTNIPLSMGY
                     PAICLGLTHGGSAHTTGEYIFKPPLKTGLRLMVSVIESVFAELS"
     gene            complement(1463..3589)
                     /locus_tag="VN363_02930"
     CDS             complement(1463..3589)
                     /locus_tag="VN363_02930"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetate--CoA ligase family protein"
                     /protein_id="HWQ83491.1"
                     /translation="MLDPTLAPFFSPQGVAIVGASHDPTKLGYGLSRNLVQSDYRGAI
                     HFVNIHGSELLGHPVYPSLAEVPDPVDLAVLLIPAAATPQALEACARRGIRSVILAAG
                     GFRETSLQGAALEAQCLQIARQHGIRLIGPNCIGLLDTHLPIDVTFLSPPGPTPGDIA
                     FISHSGAICAAVIDWARGQGFGLSRLVSLGNQADICESDVLAAVADDPYTRVLTLYLE
                     GVGDGRRFVEQASLVSRIKPILALKVGRYASGRKAVASHTGALAGQESAFNAAFRRAG
                     VIRADTSEELFDWARALAWCPLPRGRSVAVLTNSGGPGVTAADALEAQGLVLAALSED
                     TRAALGEFLDPSASLHNPVDMLAGASPQQFANSLRLLLADPGVDSALVILPPPPMHTA
                     GGIAKAIIPVIFTADKPVVIALMGERLIQEAVEYFRAARIPDYRFPERAVAALAILSQ
                     RAEALQRTTSQEPAAPDPTGTEPAVVRAALSNYPPSALQLSAGFLPPDAAAEILRSYG
                     LPVPLTPLAADADEAVRYAAELGYPVVLKVASPDIPHKSDVDGVRLDLKNAAAVRRAF
                     KQVTANASRAAPAARLLGAHLQRMQTAGQDVIIGTVQDPQFGPLLMFGSGGVEVEGLK
                     DVEFCLAPPTPEELERLLEKTWAGRRLKGYRQIPPADLEAVRFSLLRLAQLAVDFPGL
                     AEIEINPLRVFRDGASALDYRIRVAS"
     gene            complement(3638..>4189)
                     /locus_tag="VN363_02935"
     CDS             complement(3638..>4189)
                     /locus_tag="VN363_02935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83492.1"
                     /translation="LPDWMDTPTPRRLRLDLENALDRWVLLAYFNWSANPDKISLFPN
                     DFSLPDNPPCIAREFWTGGLYSLSSETAAEITIPPHGVRLFAARLFSPDVPAYLGGDI
                     HISQGLEVSGWQVAETSDHHTLEISLERPGLTQGCIDLYLPTPPHQAFLNGKPLPASE
                     LQPRCYRFNLDFDGSAGILIQYS"
BASE COUNT          868 a         1161 c         1403 g          757 t
ORIGIN      
        1 gccgaagccc tgtcagtcta tggctgcgaa ttgatcgtga tcaagcgtgg tgagaatgga
       61 caatatcttt atgatgccgc tacccggtcg tgttgggaaa tcccgccgta tccagcccgg
      121 atggttgacc cgaccggagc cggggatgca ctgtgcggag gctttctggc aggttaccgc
      181 cggacgtatg acccgctgga ggcagtgctg cacgggaaca tatcagcttc cctggtaatc
      241 gagggaagca aaccgaacta cgccctggac gcgctgccgg gattggcgga agcacggctg
      301 caagcactgc gccaggcagt gaggaaacta tgagcctgct gatcccggat ctccccgagc
      361 gcgtcctgca agaagcgatt accatccagc agatcccggc cccgactcac gcagaaacgg
      421 cgcgttcccg ctatgtgttc cagcggtttg tcgaggaagg tctggcggat gtgcagatgg
      481 actcaccggg gaatgtttac ggatgtttgc cagggagcgg gacaggcttg ccagtggtga
      541 tcagcgctca tctggatacg gtctttccgg cagatacgga cctgacctta cgcccggagc
      601 aggaccgctt atatgggccg ggaatcggcg acaattcggt cggggtcgcc gggttgtttg
      661 ggattgtgtg ggaattgcgc gcccgccacg gcgatccggg cggacctgcg acccgcctgc
      721 cgggggatgt gtggctggta gcgaatgttt gcgaggaagg tctgggcaat cttaccggga
      781 tgcgagctgt ggtggagcgt tttggcgctg aggttgccgc gtatctggtc ctggaaggga
      841 tgtcgttggg gcagatctat catcgcgggc tgggtgtgaa gcgctatcgc atccgggttc
      901 agacggcagg gggacactcc tgggtggatt atgggcagcc ttcggcagtc aatgagctgg
      961 ctcagcttat ctgcgagctt acccggctgc ctctccccgc ccggccgcgc accactttga
     1021 atgtgggggt gatcaccggt gggacgacgg tcaatacgat tgcagcgcgg gctgaaatgg
     1081 agctggacct gcgctcggaa gatccgcagg tcctgcaaga gctggcaagc caggtcgaga
     1141 cactgatcca acgggcgaac cgccccgatg tcaaagtgac cggcgaagtg attggagacc
     1201 gcccggtagg taagatctct gcccggcatc cactggtgca gctggcgaaa acctgcctga
     1261 gcgagctggg gatcgaggca aatttgggga ttggctcgac ggatacgaat atcccattga
     1321 gcatgggcta cccggccatc tgcctggggc ttacccatgg gggatcggcc cacacgacgg
     1381 gcgaatatat cttcaagcct cccctgaaaa caggcctgcg tttgatggtg agcgtgatcg
     1441 agtcagtatt tgccgagctg tcttagctgg cgacgcggat gcgatagtcc agggcggagg
     1501 ctccgtctct gaagacgcgc aaaggattga tctcaatttc tgccagcccg ggaaaatcga
     1561 ctgccagctg cgccagccgc agcagggaga aacgcaccgc ttcaaggtca gcagggggaa
     1621 tctggcggta gccttttaac ctgcgcccgg cccaggtttt ttcgagcaag cgctccagct
     1681 cctccggcgt aggcggcgca aggcaaaatt ctacatcctt caagccttct acttccacac
     1741 ctcctgagcc aaacatcaga aggggaccaa actgagggtc ttggacggtt ccaatgatca
     1801 catcctgccc ggcagtctgc atgcgctgca ggtgcgcgcc caacaggcgt gcggcaggcg
     1861 cggcgcggct ggcattcgca gtgacttgtt taaaggcacg ccggacagca gcggcgtttt
     1921 ttaaatccag gcgcaccccg tccacatccg atttatgggg gatatcggga gaggccactt
     1981 tcaaaacaac cggatagcct aattcagcag cataccgcac ggcttcatcc gcatccgcag
     2041 cgaggggcgt gagcggcacg ggcagaccat atgaacggag aatttctgcg gcggcatcgg
     2101 gcgggaggaa acccgcggat agctggagag ctgacggggg ataattggac agggcagccc
     2161 ggacgacggc cggctctgtt ccggtcgggt cgggagccgc aggctcctgg ctggtggtgc
     2221 gctgcagggc ttcggcgcgt tgggagagga tcgccagcgc ggcgacggcg cgctcaggga
     2281 aacgataatc aggaatgcgg gcagcccgaa aatactcaac cgcttcctgg atcaggcgct
     2341 cgcccatcag ggcaatgacg actggcttat cggcggtaaa gatcaccggg ataatggctt
     2401 tggcaatgcc acctgcggtg tgcatgggcg ggggtgggag gatgaccaag gcgctgtcca
     2461 ctccgggatc agccagcagc aggcgcaggc tgttggcgaa ctgctgggga gaggctccag
     2521 cgagcatatc gaccgggttg tgcaggctgg cggaaggatc gagaaattcg cccagggcgg
     2581 cccgggtgtc ttccgataag gctgccagaa ccagaccctg ggcttccagg gcatcggcag
     2641 cggtgacgcc agggccgccg gaattggtaa gcacggccac ggaccgcccg cggggaagtg
     2701 gacaccaggc cagggcgcgc gcccaatcga ataattcttc gctggtatcg gcgcggatca
     2761 ctccggcgcg gcggaaggcg gcattgaaag cgctttcctg ccctgccagg gcgccggtgt
     2821 gggaggcgac tgcctttcgc ccgctggcat accggccaac cttgagagct aaaatgggtt
     2881 ttatccggct tacgaggctg gcctgttcga caaagcgccg cccatccccg actccttcaa
     2941 ggtaaagggt caggacgcgg gtataaggat cgtctgctac tgcagccagc acatcgcttt
     3001 cacaaatatc ggcctggttg cccaggctga ccaggcgcga gaggccaaaa ccctggccgc
     3061 gcgcccaatc gataacggcc gcgcaaatgg caccagaatg cgaaataaag gcaatgtcgc
     3121 cgggtgttgg gccaggcggg gagagaaaag tgacgtcgat cggcaggtgg gtatccaaca
     3181 ggccgatgca attcgggcca atcaggcgga tgccatgctg gcgggcgatc tggagacact
     3241 gcgcttccag ggccgcaccc tggagtgatg tctcccgaaa accgccggca gccaggatca
     3301 ctgaccggat accccgcctg gcacaggctt ccagggcctg cggtgtggca gccgccggga
     3361 tgagcaggac tgccagatct accggatcag ggacctcagc cagggatgga tatacgggat
     3421 gcccgagcag ctcgctgccg tggatattca cgaagtggat cgccccgcga taatcgctct
     3481 gtaccaggtt gcgcgagagc ccgtaaccga gcttggtggg atcgtgggag gcgccgacaa
     3541 tggcgacccc ctgtggggag aaaaacggcg ccagggttgg gtcgagcata ggtttccctc
     3601 cggtctgctg agtaatccgg cgtacaggtc cgccggttta cgaatactgg atcaggatgc
     3661 cggcactgcc atcgaagtcc agattgaagc gataacaacg gggttgaagc tcagaagctg
     3721 gcagcggctt cccattgaga aatgcctggt ggggtggggt gggcaaatac aggtcgatac
     3781 agccctgcgt aagcccggga cgctccaaag agatttccag cgtatgatga tcggaagttt
     3841 cagcaacctg ccagccgctg acttccaggc cctgcgagat atggatatcg cctccgagat
     3901 aagcaggaac atctggcgaa aaaagccgtg cggcgaataa acgtacccca tggggaggaa
     3961 tcgtaatttc tgcggctgtc tcgctggaga gtgaatacaa tcctccggtc caaaactcac
     4021 gggcaatgca cggcggattg tcgggcaggc tgaagtcgtt gggaaacagg ctgatcttat
     4081 cggggttagc tgaccaattg aaataagcca gcagaaccca gcggtccagc gcattttcca
     4141 gatcaaggcg caggcgccgg ggggtggggg tgtccatcca gtcggggag
//
LOCUS       DATMXK010000159         3835 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_48074, whole genome shotgun sequence.
ACCESSION   DATMXK010000159 DATMXK010000000
VERSION     DATMXK010000159.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3835)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3835)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3835
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48074"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            646..2487
                     /gene="glmS"
                     /locus_tag="VN363_02940"
     CDS             646..2487
                     /gene="glmS"
                     /locus_tag="VN363_02940"
                     /EC_number="2.6.1.16"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004360 - glutamine-fructose-6-phosphate
                     transaminase (isomerizing) activity [Evidence IEA];
                     GO_process: GO:0006040 - amino sugar metabolic process
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine--fructose-6-phosphate transaminase
                     (isomerizing)"
                     /protein_id="HWQ83493.1"
                     /translation="MCGIVGYIGPQDATPIILNGLKRLEYRGYDSAGLAVLENSHIEV
                     RRDAGKLSHLATLVHDLPVHGHVGIGHTRWATHGEPNARNAHPHMGMTGEVVVVHNGI
                     VENYLALREDLQAEGVVFKSETDSEVIVHLVERYLGAGYVLAEAARLALGHLKGAHGV
                     VLLSSREPDKIVAARIGNAGGVVLGLGKDEMYVASDLPAILDHTRRVVFLESGQMATI
                     TRQGLALQTLTGAGLPYEVHTIAWDPVSAEKGEYRHFMEKEIHEQVRSLTDTIGGRVD
                     FDQGRILLPELNLTPELARSIKKITIVACGTAWHAGTVGKVLIEKIARLPVEVDIASE
                     FRYRDPIVDSDTVILAISQSGETADTLAAMEEGRSKGARLWSIVNAIGSQAMRIADGY
                     ISMLSGPEIGVASTKAYTAPLVDLYMLAILLGDLRGVLDESTRRRLIADLRLVPDLAG
                     RCLDREAEVEKVARVLLNVTNCLYLGRGINMPTASEGALKLKELSYIHAEGYPAGEMK
                     HGPIALIDRDLPVVVIAPRDPWYDKMLSQIEQAKARGGKVIAVATDGDEVIASKADHV
                     LWVPETPWMLSPVTTVIPLQMLAYHIAALRGLDVDQPRNLAKSVTVE"
     gene            complement(2542..>3835)
                     /locus_tag="VN363_02945"
     CDS             complement(2542..>3835)
                     /locus_tag="VN363_02945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83494.1"
                     /translation="PLIRTLQLDSLIEFTDQAKLLEGSYRFLRRIDYIDAEQNPTSTY
                     ETLYGFFWINLEAGYCVIHGREPSILRTLETAIEAGAGINLVNLIITTELKNSLPFLL
                     EESLRSIRLHDPDPESSRFRWLTIADEAPYSKGYRELEASYPEVRDARYRISIDEQRE
                     TSLNIRFDRGVISLGGKLSATQFRKWAINSLGEIITKVQSLHTQPTLFIKTRGFRSVP
                     ELARYTAAQKDSILRLAADILELKVQPGIGYRTTGISPLTLANRMQRLMRAYLYIACP
                     QEGCDQEGYFACEVCGGTNFQVVRRESGLAIKCPRHPGQPWSAAAPIPSACLNGHTLA
                     IDLPMLEENLLLLPAPQLLNTISELVGKYIPVYSFDPKLERFYIRERRVYYTAEAAIP
                     EPVQPVMIFNNIRVGSVAPGGTVTGVTLDGSSPAIIQN"
BASE COUNT          878 a          970 c         1091 g          896 t
ORIGIN      
        1 cccacaaaca aaacattgat gagcattcgg ttgaggagtg ggcataattg tgctccggct
       61 agatgggatt ctgatagatc accgatgatc aatagccgct ggaagttttg gctggttctg
      121 gcatcgatcg gatctgacta attatactat ccggtaaact ataaggttaa taacccactt
      181 gattaagctg taagacaggc tcgcgggtca gtaggatcgg aaccaccaaa aaatacggta
      241 taatgcgcac acactgaatt taaggaggtg agtcgataag tctttctctg agggaagaca
      301 gcgcatggcc cctgatttat ccggggtgac gaggatgagg gttcctgccc gcgagggtag
      361 gttaaccgga tgaagccaac aacgagttcg ccaaagagct gatgagaagg caaacagcct
      421 tcgtccggga aaatcgaagg atcagcggaa gccctctggg tgtgctacca cccaatttct
      481 ttccctccca aacaacatac agcctgaatg cctgccaggt aactggtcgg acaaaatact
      541 gtgtgagtgg caatctgctg ccgtcacccg gccggcaggg taatttaccc tgacgctggt
      601 tgattggccg catattcgga caggatcata tggagggatt tctatatgtg tggaatagtt
      661 ggatatattg gaccgcagga tgctacccct atcattctga atggccttaa acgcctggaa
      721 taccggggct acgactcggc tggactggct gtgctcgaaa actcgcatat cgaagtccgg
      781 cgggacgccg gtaaattatc ccacctggca accctggtgc atgacctgcc ggtgcatggt
      841 catgttggca tcgggcacac ccgttgggcg acccatggtg agccgaatgc tcggaacgcc
      901 catccgcata tgggtatgac cggtgaggtc gttgttgtcc ataatgggat tgtagaaaat
      961 tacctcgctc tgcgagaaga tttacaggcc gaaggtgtgg tcttcaaatc tgaaaccgat
     1021 agtgaagtga tcgtccatct ggttgagcgc tatctgggtg caggctatgt tttggccgag
     1081 gctgcccggc tggcgctggg tcatttaaaa ggcgcccatg gagtggtttt actctcatcc
     1141 cgtgagccgg ataagatcgt cgcggcccgg attggcaatg ccgggggcgt cgttctgggt
     1201 ctgggtaagg acgagatgta tgtggcttcc gaccttccgg cgatcctcga tcacacccgg
     1261 cgggttgtat ttctcgaatc aggccagatg gcgacgatca cccgccaggg gttggcattg
     1321 caaactctta ccggagctgg actgccgtat gaggttcaca ccattgcctg ggacccggtg
     1381 tcggcggaga aaggggaata ccgccatttt atggagaaag agatccacga gcaggtccgt
     1441 tcgctcaccg ataccattgg cgggcgagtt gattttgacc aggggcgaat tttgctgcca
     1501 gaattgaatc tgacccccga gcttgcccgc agcatcaaga aaataaccat tgtcgcctgc
     1561 ggtacggcct ggcatgccgg cacggtgggt aaggtgctga tcgaaaagat tgcccggttg
     1621 cctgttgaag ttgacatcgc ttccgagttt cgctaccgtg atccaattgt ggatagtgat
     1681 acggtcatcc tggcgatcag ccagtctggc gagactgccg ataccctggc ggcaatggaa
     1741 gaagggcgct cgaaaggcgc tcgtttgtgg tctattgtca atgcgattgg ctcacaggcc
     1801 atgcgcatcg ccgatggata tatctccatg ctcagcggac ccgaaatcgg ggttgcttct
     1861 accaaggctt atactgcgcc cctggtggac ctgtacatgc tggcgatctt gctgggcgat
     1921 ctgcgcgggg tattggatga aagcacgcgc cgccgcctga ttgccgatct ccgcctggtt
     1981 ccggatcttg ccggacgctg cctggaccgg gaagccgaag tggaaaaagt cgcccgtgtc
     2041 ctgttgaatg tcacaaactg cctgtatctt gggcgaggca ttaacatgcc taccgccagt
     2101 gaaggtgcgc tcaagctgaa agagttatct tacattcatg cagaaggtta tccagccggc
     2161 gagatgaaac atggacccat tgcactgatc gaccgtgatt tgccggtggt agttattgcc
     2221 cctcgcgatc cgtggtatga caagatgctt agccagattg agcaagccaa ggcgcgcggc
     2281 ggaaaggtca ttgctgttgc taccgatggg gatgaggtga tcgcttcaaa ggcagatcac
     2341 gtcctctggg tacccgagac accctggatg ttgagccctg ttaccactgt catcccgctg
     2401 caaatgctgg catatcatat cgccgcctta cgcgggctgg atgtcgacca accgcgtaac
     2461 ctggcgaaat cggtgacggt agaataattc cgtatcccgt agaattagtc ccggggtcat
     2521 gtggtctgct ggtcagaata attaattctg aataattgct ggactgcttc catccaacgt
     2581 gaccccggtc accgttcccc ctggtgcgac tgatccaacg cgaatattgt taaatatcat
     2641 caccggttgc actggttctg ggatggctgc ctcggcggta tagtaaacgc gccgctcgcg
     2701 gatgtaaaaa cgctccagct tcgggtcaaa gctatataca gggatatatt ttccaaccag
     2761 ctctgagatc gtgttcaata attgtggggc gggtaataaa agtaaattct cctccagcat
     2821 gggcaagtcg attgcaagcg tgtgtccgtt caagcacgca ctcgggattg gagctgcagc
     2881 tgaccagggc tgtccagggt gacggggaca tttaatggca agaccgcttt cccgcctgac
     2941 aacctgaaaa ttcgtgcctc cgcacacctc gcatgcaaaa taaccttcct gatcacaacc
     3001 ctcctgagga caggctatat acagataagc ccgcatcaga cgctgcatgc ggttcgccaa
     3061 tgtcagcggt gagataccgg ttgttcgata tccaatcccg ggttggactt tcaactcaag
     3121 gatatcggca gctaaccgca ggatactgtc tttttgagcc gctgtatacc gagccagttc
     3181 gggtacactg cgaaagccgc gagtcttgat aaacagggtg ggctgggtgt gaaggctctg
     3241 cactttcgtg atgatctcac ccagactgtt gatggcccat ttgcgaaatt gcgtggcgct
     3301 caacttgcca cccaggctga tcacaccgcg atcgaaccgg atgttgaggg aagtctcccg
     3361 ctgctcgtcg atcgaaatgc ggtagcgggc atcgcgcact tcaggatagc tggcttctag
     3421 ctcgcgataa cctttggaat aaggcgcttc atcggcaatg gtcagccagc gaaaacggct
     3481 gctttccgga tctggatcat gcaggcggat cgaacggagc gattcttcca gcaagaacgg
     3541 gagcgaattt tttagctcgg tcgtgataat caaatttacc agattgattc ccgccccagc
     3601 ctcgatggct gtttccagag tgcgcaagat gctgggctct cgcccgtgaa tcacacagta
     3661 accagcttcc agattgatcc agaaaaagcc atacagggtt tcatacgttg aagtgggatt
     3721 ttgctcggca tcgatgtagt cgatccggcg gagaaaacgg tagctgcctt ccagcaattt
     3781 tgcttgatcg gtaaattcga tcagtgagtc cagctgcagg gtacggatta agggc
//
LOCUS       DATMXK010000160         2664 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_129486, whole genome shotgun sequence.
ACCESSION   DATMXK010000160 DATMXK010000000
VERSION     DATMXK010000160.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2664)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2664)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2664
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129486"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            129..608
                     /locus_tag="VN363_02950"
     CDS             129..608
                     /locus_tag="VN363_02950"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA deacylase"
                     /protein_id="HWQ83495.1"
                     /translation="MPIRNNVTRLLESRKITFTACELPPEKLGAVEAAHILGVPPEQV
                     YKTIVVKREGKGKPILAVIPGPDEVDLKAVAKILGEKKVLLPTEREAEQLTGLQAGGI
                     SPLALINKGFQVLVDESAQALGEIYISGGQRGLNIRLALGDLLRLTNARLAVITVQA"
     gene            728..1486
                     /locus_tag="VN363_02955"
     CDS             728..1486
                     /locus_tag="VN363_02955"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558848.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter ATP-binding protein"
                     /protein_id="HWQ83496.1"
                     /translation="MSDQPQPADPIIVCRDVHKWYGQFHALRGITMDVGRGEVVVVFG
                     PSGSGKSTFIRCLNRLEEHQDGQIFVDGTELTSNLRNIERVRMETGMVFQQFNLFPHM
                     TVIDNITLAPVQVRKWSKEKAVEIGKQLLERVGIPEQANKFPSELSGGQQQRVAIARA
                     LAMQPKVMLFDEPTSALDPEMIKEVLDVMVGLAKTGMTMVVVTHEMGFARGVANRMVF
                     FDQGVIVESGPPLQIFNNPQEDRTKLFLSQILTH"
     gene            complement(1563..>2664)
                     /locus_tag="VN363_02960"
     CDS             complement(1563..>2664)
                     /locus_tag="VN363_02960"
                     /EC_number="3.2.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="sialidase family protein"
                     /protein_id="HWQ83497.1"
                     /translation="PGTPGGVSTPESAANPPDFTYAGTVVVDRGAIGFAVPHQRYGFA
                     GEFVDKEWMEIDRYPESPCFGNVYVSFTPFHGVGGNSHIRFSRSTDGGASFSNPLPVN
                     TGGQAGSTYNQGSDIAVGPDGAVYVAYIAYPSGGETSASVRIAKSSDCGQHWDNPVIA
                     GSLTTVQAPGVVFRTPTFAFTAVDDTNPDTVYVAYQSFEGGSYNIYTRRSLDGGATWG
                     DAVMVNDDGGTRHQIFPTIETSNGALHVAWYDFRNSVTAANEALDVYYACANCAGVAY
                     PAFSHSERVTDISHNGNCRMFGGGSSAFHGDYNELAAIWDGSQHTVHVAWADNRDVPA
                     AECDLDPAVGPPTNNIGNRNQNIYADKLLVAP"
BASE COUNT          572 a          772 c          793 g          527 t
ORIGIN      
        1 gggcggcagc ggtctgggcc tggcgatcgc ccgccagttc gccgaagccc accatggggt
       61 cctgactgcc gccaaccatc ctgagggcgg agccatcttc accctggtgc tgccactgga
      121 ttaagccgat gcccatccgc aataatgtca cccgcctgct ggagagccgg aaaattacct
      181 tcacagcctg cgagcttccg cccgagaagc tgggcgccgt ggaagctgcc cacatcctgg
      241 gcgtcccccc cgaacaggtg tataagacca ttgtcgtaaa acgcgaaggc aaaggcaagc
      301 ccatcctggc agtcattcca ggacccgatg aagtggactt gaaagcggta gccaaaattc
      361 tgggtgagaa gaaggtcctc ttgccgaccg agcgcgaggc agaacagctc accgggctgc
      421 aggcgggtgg aatttcaccc ctggccttga ttaacaaagg cttccaggtg ctggttgatg
      481 aatcggccca ggcactgggc gagatttata tctccggcgg gcagcgcggt ctgaatatcc
      541 gcctggcgct gggcgatctt ttgaggttga ccaacgccag gctggcggta atcaccgttc
      601 aagcctgacc aacccggcat tcgcgcctgc cgcggggtgc cggcgggttt gagggcttgc
      661 cgattcggga aatcaattta taatacgaca accttttcgt ttattcgctg ttaaccagga
      721 gaaatccgtg agcgatcaac cgcagcccgc cgacccgatc attgtctgcc gcgatgtgca
      781 taaatggtat ggacagtttc acgccctgcg cggcatcacc atggatgtcg ggcgcggtga
      841 agttgtggtc gtctttggcc cgtctggctc aggtaaatcg accttcattc gctgcctcaa
      901 ccggctggaa gagcaccagg atggccagat tttcgtcgat ggcaccgagc tgaccagcaa
      961 cctgcggaat atcgaacggg tgcgtatgga aaccggtatg gtcttccagc agttcaacct
     1021 gttcccccac atgaccgtga tcgataatat caccctggcg ccggtccagg tgcgtaaatg
     1081 gtccaaagaa aaagcggttg agatcggtaa gcagctcctg gagcgcgtcg ggatccccga
     1141 gcaggccaac aaattcccct ccgagctgtc cggtgggcag cagcagcgcg tggccattgc
     1201 ccgcgccctg gcgatgcagc ccaaagtcat gctcttcgac gagccgacct ccgccctgga
     1261 cccggaaatg atcaaagaag tcctggatgt gatggtcggg ctggcgaaga ccgggatgac
     1321 catggtcgtg gtgacgcatg aaatggggtt tgcgcgcggc gtcgctaacc gcatggtgtt
     1381 cttcgaccag ggtgtgatcg tagaaagcgg tccacctcta cagatcttca ataaccccca
     1441 ggaagatcgc acgaaattgt tcttatccca gatcctaacc cactgagcgc ccggtgtcag
     1501 gcaagacaaa ccaggacttt tatctgcgga taaaagtcct ggtctgattt taatgtttat
     1561 ggttacggag caaccagcag tttatcggcg tagatattct gattgcgatt gccaatattg
     1621 ttggtcggcg ggcccacggc cgggtcgaga tcgcattcag cggctggcac atcgcggtta
     1681 tcagcccagg ccacgtgaac ggtatgctgc gatccatccc agattgcagc cagctcgtta
     1741 taatcgccgt ggaaagcgga cgagccgcca ccaaacatgc ggcagttgcc attgtgggag
     1801 atgtctgtga cccgctcgct gtggctgaat gccgggtaag ccaccccggc acagttcgcg
     1861 caggcataat acacgtccag ggcctcgttg gcagcggtta cactgttgcg aaaatcgtac
     1921 caggcgacat gcagggcgcc gttggaggtt tcaatggtcg gaaagatctg gtggcgggtg
     1981 cccccgtcat cgttgaccat gacggcgtcg ccccaggtcg ctccgccatc cagcgagcgc
     2041 cgggtataga tattgtagct gccgccttca aagctctggt acgccacgta gacggtatcc
     2101 ggattggtat catcgactgc agtgaatgca aaagtgggcg tgcggaagac taccccgggc
     2161 gcctggacag tcgtaagcga gccggcgatg acgggattgt cccaatgctg gccgcaatcg
     2221 ctggattttg cgatgcggac cgaggccgag gtctccccac cggagggata agcgatatag
     2281 gcaacataca ctgcgccatc cgggcccacg gcgatatccg aaccctggtt ataggtgctc
     2341 ccggcctgcc cgccagtatt gacaggcagc gggttggaaa agctggcgcc cccatctgtc
     2401 gaacggctga agcggatatg gctgttgcca cccaccccat ggaagggggt aaagctcaca
     2461 tacacattcc cgaaacaggg gctttccgga tagcgatcga tctccatcca ttctttatcc
     2521 acaaactcac ccgcaaatcc gtaacgctgg tggggaactg caaaaccgat tgccccgcga
     2581 tcgaccacca cggtaccggc ataggtgaaa tcaggcggat tggcagccga ttcaggcgtg
     2641 ctgacgccgc caggtgtgcc aggg
//
LOCUS       DATMXK010000161         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_74967, whole genome shotgun sequence.
ACCESSION   DATMXK010000161 DATMXK010000000
VERSION     DATMXK010000161.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74967"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..598
                     /locus_tag="VN363_02965"
     CDS             <1..598
                     /locus_tag="VN363_02965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83498.1"
                     /translation="PGINARGGKFIDLQVENKMKKITFSLLVLVLLASCSSTRTKTYE
                     YPTFTMKYPASWKTMAEISPTYKKGQEYMWLHINEDFTLTSAKKEGDPGVYLTVSARA
                     HGDNLTFSYWTYATVADYTRNLNQVPVTVGGVDGVMYHYDREWEGQWYQFRDYWFNYQ
                     MLSYYLSFRAENLDDYQSEIDLILESFTLKEWDPFDKH"
     gene            585..1112
                     /locus_tag="VN363_02970"
     CDS             585..1112
                     /locus_tag="VN363_02970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83499.1"
                     /translation="MTNINRLLLLTILLTACSPAGQQFDYYTLTFTYPKSWRPMGEVS
                     ESYQSGGEFMWMGIQEDLTVTSVKADGEPGAYFSVASAEATDWSIDSDVDFMYAMNES
                     DMRNYAERPITVAGIEGLSATYERNWQNQWYQFHDVWIRHGSLFYLLSFRAADLDSQR
                     AAMDMILESFTFTEK"
     gene            complement(1179..1703)
                     /locus_tag="VN363_02975"
     CDS             complement(1179..1703)
                     /locus_tag="VN363_02975"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ83500.1"
                     /translation="MSFYVRSIYSDLSAEERQKLLALRSDAEGAASQIIAEGGQAIPF
                     YGDVADYDTAGRLVQTALEAYGQVDILVNNAAGLGTGMVMHLSEKEWDYQTVPKLKGA
                     FNTMNHAVPHMIKQQFGRVINIASDAWIGMNGMSAYSAANAGLVGWTKASAKDLFKLG
                     ITVNAVCPQAASPV"
     gene            1754..>2369
                     /locus_tag="VN363_02980"
     CDS             1754..>2369
                     /locus_tag="VN363_02980"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWQ83501.1"
                     /translation="MPPETISIAEARRIMLTAQGLAHGLAQPPPRKAEKADVLAMIRR
                     MNLLQIDTINVIARSPYLVLWSRLGDYPPAWVDELLEEGALFEYWAHALCFLPIEDFP
                     LYRRRMLDGVRGWGDTDEWIRQHAGTVAMILQRIREEGGLRSADFKNDHHPAGGWWNW
                     KEEKTALEVLFIRGDLMSARRQRFQRVYDLPERVLPGWEDGPEYA"
BASE COUNT          533 a          647 c          703 g          486 t
ORIGIN      
        1 ccccgggatt aacgcccggg gtggaaaatt tattgacctg caggtggaaa acaaaatgaa
       61 aaaaataact ttttcgctgc tggtactcgt cttgctcgct tcttgctctt caacgagaac
      121 aaaaacatat gagtacccca cctttaccat gaaatatccc gccagttgga agacgatggc
      181 tgagatctcg ccgacgtata aaaagggcca agaatacatg tggctgcaca tcaacgaaga
      241 cttcaccctg accagcgcaa aaaaagaggg cgacccaggg gtatatctga ctgtgtcggc
      301 cagggcgcac ggcgacaatc tgacattctc ctattggacg tatgcgacag tggcagacta
      361 tacccggaat ttgaaccagg ttccggtaac agtgggcggc gtggatggcg tgatgtacca
      421 ctacgaccgc gaatgggaag gtcagtggta ccagttccgg gattactggt tcaattatca
      481 gatgttatcc tactatctgt cttttcgggc cgaaaatctg gatgactacc agagcgaaat
      541 cgacctgatc ctggaaagtt tcactcttaa agagtgggat ccctttgaca aacattaacc
      601 ggctgcttct cctgacgatc ctgctcacag cctgctcccc ggctggccag cagttcgatt
      661 attacaccct gactttcacc tacccaaaaa gctggaggcc gatgggggag gtatcggaga
      721 gctaccagag cgggggcgag ttcatgtgga tgggcatcca ggaagacctg acggtaacca
      781 gcgtgaaagc agatggcgag ccaggggcat atttttcggt ggcctcggcg gaggcaaccg
      841 attggagtat cgattcggat gtcgatttca tgtatgccat gaacgaatcg gatatgcgca
      901 attacgctga aagaccgatc accgtggcag gaatcgaagg cctgtcggct acttacgaac
      961 gcaactggca gaaccagtgg taccagttcc acgatgtctg gatccggcac ggctcgctgt
     1021 tctacctgct atccttccgc gctgctgacc tggacagcca gcgcgccgcg atggacatga
     1081 tcctggagag ttttaccttc acggaaaaat aatcctgccc gaagattgcc tggctcgatc
     1141 ccaatcgaaa tattaatgga gccaatcacc gcccgaaatt acaccggcga agccgcctgg
     1201 gggcagacag cattgacggt gatgcccagc ttgaacagat ctttggcgct ggctttggtc
     1261 cagcccacca gcccagcgtt ggcggcgctg taggcgctca tgccgttcat gccgatccag
     1321 gcgtcggagg caatattgat cacccgtccg aactgctgct tgatcatatg cggaacggcg
     1381 tggttcatgg tgttaaaagc gcccttgagc ttgggaacgg tctggtaatc ccactctttt
     1441 tcggacaggt gcatcaccat gccggtgccc aatccggcgg cgttattgac caggatgtcc
     1501 acctgcccat aagcctcgag cgcggtctgg accagccggc cggcggtatc ataatcggcc
     1561 acatcgccgt agaatgggat cgcctgcccg ccctcggcga tgatctggct ggcggcgccc
     1621 tcagcgtcgc tgcgcagggc gagtaatttc tggcgctctt cggcggataa atctgaatat
     1681 atagaccgga cataaaaaga caagtacaat ccggtggaat ttgttgggat aatgcggtta
     1741 aaatcgaaga attatgcccc ctgaaactat ttccatagcc gaagcccgcc ggatcatgct
     1801 cactgctcag gggctagccc acgggctggc tcagccgccg ccgcgcaagg ctgaaaaagc
     1861 ggatgtgctg gcgatgatcc ggcggatgaa cctgctgcag atcgatacga tcaatgtgat
     1921 cgcccgcagc ccgtacctgg tgctgtggag ccggctgggg gattacccgc cggcctgggt
     1981 ggacgagctg ctggaggagg gggcgctgtt cgagtactgg gcgcacgccc tgtgcttcct
     2041 gcccattgaa gattttccgc tctaccgccg gcgcatgctg gacggggtgc gcggctgggg
     2101 ggatacggac gaatggatcc gccagcacgc cgggacggtg gcgatgatcc tgcagcgcat
     2161 ccgcgaagaa ggcgggctgc gttcggcgga tttcaaaaac gaccaccatc ccgccggggg
     2221 ctggtggaac tggaaggaag aaaagactgc cctggaggtg ctgttcatcc gcggcgatct
     2281 gatgagcgcc cgccgccagc ggttccagcg cgtgtacgac ctgcccgagc gggtgctgcc
     2341 gggctgggaa gatgggcccg agtatgcca
//
LOCUS       DATMXK010000162         2974 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_7020, whole genome shotgun sequence.
ACCESSION   DATMXK010000162 DATMXK010000000
VERSION     DATMXK010000162.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2974)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2974)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2974
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7020"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..250)
                     /locus_tag="VN363_02985"
     CDS             complement(<1..250)
                     /locus_tag="VN363_02985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559882.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglucomutase/phosphomannomutase family
                     protein"
                     /protein_id="HWQ83502.1"
                     /translation="MSFKIKFGTDGWRGRIADDFTFSNVRRCAQGFAAYLIQEGHAGA
                     AVVVGFDKRFQSEHFAASVAEVLAGNGLQVYLTEEATPT"
     gene            439..1602
                     /locus_tag="VN363_02990"
     CDS             439..1602
                     /locus_tag="VN363_02990"
                     /inference="COORDINATES: protein motif:HMM:NF024925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-grasp domain-containing protein"
                     /protein_id="HWQ83503.1"
                     /translation="MNLVYLSPHFPPNYVQFSQQLSYLGVNVLGLADEPYDQLPHNLK
                     SSLAVYYRVNDMHNYDELVRALGYFTHQYGKVDRIDSLNEYWLETEACLRLDFNIPGL
                     QGEDLARAKRKSRMKEVFRKAGVAVARGKVVQTFEEAKRLVRQTGYPLIAKPDIGVGA
                     AKTYKIHNLTELEGFFISKPAVDYILEEFIAGKIVTFDGLTDREGNPVYVNSLVYDKG
                     ILEAVNQDEHMYYYTQRDIPADLEDAGLRVLRAFNVCERFFHFEFFRRPDNGEIVALE
                     VNLRPPGGMTTDMWNYASDIDIYREWAHIIVHNRFTADYTRKFHVAFVGRKYRYAYAT
                     PHADLVAELGPALVHHEPIEGIFSAALGDYGYLVRSPELSEVLDMAKFIQALA"
     gene            1642..>2974
                     /locus_tag="VN363_02995"
     CDS             1642..>2974
                     /locus_tag="VN363_02995"
                     /inference="COORDINATES: protein motif:HMM:NF023649.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3604 domain-containing protein"
                     /protein_id="HWQ83504.1"
                     /translation="MISSAFEGLQPYYGDLHNHCSVGYGHGSAQDAFHNARLQLDFVA
                     VTPHAHWPDIPAGETRLESLVAYHQKGFERTQAEWEDFQKLVEAHNEPGKLVTFLGYE
                     WHSLEYGDHHILYNGSQGPVLREPSLVGMRARLRQLNQKGVEGMLIPHHIGYKAGFRG
                     INWETFDPEFSPLVEIMSMHGASESDRTGYPYLHSMGPRDGQSTYQYGLSQGKIAGVL
                     GSTDHHSAHPGSYGHGRAGVWAVSLTRADIWEALKARRTYALTGDRIQLAYSLNGEPM
                     GSLVPQVAERQVDIRVEAGEAIDTIELIYNNRVLQRWSILDGEPPGLDWQGRCKVTLE
                     LGWDEKNRDVDWDVELQISGGSLVGIEPRFRGHDIVAPQAGEQDSYAFSQWQRAGENG
                     VVFKTRTWGNPTTTTSNTQAMSLEIEGDPDTRLFARLNHQEITLSLGELLLR"
BASE COUNT          641 a          862 c          839 g          632 t
ORIGIN      
        1 gtgtcggcgt cgcctcttct gtcagataca cctgcaggcc atttcccgcc aggacttcag
       61 ccacgctggc tgcgaaatgt tcggactgga agcgcttatc aaaaccgacc acgaccgctg
      121 ccccggcatg cccttcctga atcagataag ctgcaaaccc ctgcgcacag cggcggacat
      181 tggaaaaagt aaaatcatct gcgatgcggc ctcgccagcc atcggtgcca aatttgatct
      241 tgaaactcat ccgccctccc aaaggtcggt tttgtgaacc acggtattat atcaagggtt
      301 gggtctattt gattatgggt ccaatatctg gtgaatcttc cagccatctg gtgcattccg
      361 ggttgcacaa atcatctctt aggcgtataa atagccagat cctggttagc gctcacgcac
      421 cagagcagga gcaatccgat gaacctggtt tacctctcac cgcactttcc tccaaactac
      481 gtccagttct ctcagcagct gtcttacctg ggagtgaatg tgcttggtct ggcggacgag
      541 ccttacgacc agttacctca taaccttaaa agctctctgg cagtctatta ccgcgtcaat
      601 gatatgcata attacgacga gctggtgcgc gccctgggct acttcaccca ccagtacggg
      661 aaggttgacc gcatcgattc gctgaatgaa tactggctgg aaactgaagc ctgcctgcgc
      721 ctggatttca acatccccgg cttgcagggc gaagacctgg cgcgggcgaa acgtaaatcg
      781 cgcatgaaag aggtatttcg aaaagccggc gtcgccgtgg cgcgcggcaa agtcgtgcaa
      841 acctttgaag aggcgaaaag gttggtccgc cagaccggct acccgctgat cgccaagccg
      901 gatatcgggg tgggcgccgc gaaaacgtat aaaattcaca atttgaccga attggaagga
      961 ttcttcatct ccaaaccggc agttgattac atcctggaag aattcatcgc tggcaagatc
     1021 gtcactttcg acggcctcac cgaccgggaa ggcaatccgg tgtatgtcaa ctcgctggtg
     1081 tacgacaaag gcatcctgga agctgtcaac caggatgagc atatgtatta ctacacgcag
     1141 cgcgatattc cggccgacct ggaagacgcc gggctgcggg tgctgcgcgc cttcaatgtc
     1201 tgcgagcgtt tcttccactt cgagttcttc cgccgcccag ataacggtga aatcgtcgcc
     1261 ctggaggtca atctgcgccc gccgggcggc atgaccaccg atatgtggaa ttacgccagc
     1321 gatatcgata tctaccgcga atgggcgcat atcatcgtcc ataaccgctt caccgccgat
     1381 tacacccgca aatttcatgt cgcttttgtg gggcgcaaat atcgctacgc gtacgccacc
     1441 ccgcatgccg acctggtagc agagctcggc ccggctctgg ttcaccacga gccgatcgaa
     1501 ggtatcttca gcgctgccct gggggattat ggctacctgg tgcgttcacc ggaattaagc
     1561 gaggtactgg acatggcgaa attcattcag gcgctcgcct gaccgtcagc tccaatcgat
     1621 ccctctgaaa ggttcctggc attgattagt tccgcttttg aaggtctgca gccttattat
     1681 ggcgacctgc ataaccactg cagcgtcggc tacgggcatg gctccgcgca ggacgccttt
     1741 cacaatgctc ggctgcagct cgactttgtg gccgtgacac cccatgccca ctggccggac
     1801 atcccggccg gtgagacccg cctggaaagc ctggtggcat accatcagaa aggcttcgaa
     1861 cgcacccagg cagagtggga agactttcaa aagctcgtcg aggctcacaa tgagcctggt
     1921 aagctggtca cttttctggg gtatgaatgg cattcgctgg aatatggcga ccatcatatt
     1981 ctttacaatg gatcccaggg tccggtgctc cgggagccga gcctggtggg aatgcgcgcc
     2041 aggcttcgcc agctgaacca gaagggtgtc gaagggatgc tcatccccca ccacatcggt
     2101 tacaaagcgg gtttccgggg catcaactgg gaaacttttg acccggagtt ctctcccctg
     2161 gtcgagatca tgtccatgca cggcgcttcc gagagcgacc ggactggcta tccctatctg
     2221 cacagcatgg gaccccgcga tggccagagc acctaccagt atgggctttc ccagggcaag
     2281 atcgctggtg ttctgggctc caccgaccat cacagcgccc atcccggcag ctatggacat
     2341 ggtcgcgccg gcgtctgggc ggtttcgctg acccgggcgg atatctggga agcgttgaaa
     2401 gcccgccgga cctacgccct caccggagac cgcatccagc tggcttactc gttgaacggt
     2461 gagccgatgg gcagcctggt cccacaggtg gctgaacgtc aggttgatat ccgcgttgaa
     2521 gcaggcgagg ctatcgatac cattgagctg atttacaaca accgggtcct gcagcgctgg
     2581 agcatcctgg acggcgagcc gcctggcctg gactggcagg gacgctgtaa agttacgctg
     2641 gagctgggct gggacgaaaa aaaccgggat gtggattggg atgtggagct gcagatttcg
     2701 ggcgggtctt tggtggggat cgagcctcgc ttccgcgggc atgatatcgt cgccccgcag
     2761 gctggcgagc aggatagcta cgctttcagt cagtggcagc gtgccggaga aaatggtgtg
     2821 gttttcaaga cccgcacctg gggcaatccc accacgacga cctcaaacac ccaggctatg
     2881 agcctggaaa tcgaggggga tccagatacc cggctttttg cccggcttaa ccatcaggaa
     2941 atcacccttt ctctgggcga gctcctgcta aggg
//
LOCUS       DATMXK010000163         4884 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_93713, whole genome shotgun sequence.
ACCESSION   DATMXK010000163 DATMXK010000000
VERSION     DATMXK010000163.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4884)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4884)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4884
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93713"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1425
                     /locus_tag="VN363_03000"
     CDS             <1..1425
                     /locus_tag="VN363_03000"
                     /inference="COORDINATES: protein motif:HMM:NF019966.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase M16"
                     /protein_id="HWQ83505.1"
                     /translation="DMQILQTRQNTPDSPEALATIPVLKLADLDPKIKTIPTEIQSIS
                     GTPTLIHDLFTNGIVYLNLAFDLHTLPAAWLPFIPLFGQALVEMGTSRQDYVRLSQRI
                     GRATGGIHPDWLTSNVEKSARSAAYLFLHAKAVTNQAGELFNILNDLLLDVRLDNRER
                     FKQIVLEAKAGLEAGLVPAGHWFVNRRLRASYTEADWASEQMGGVEYLFFLRQLIDQV
                     ENHWTSVLDTLEGIRSRLVNRQAMVVNVTLDQKGWSKVQPKLTEFLERLPEGELNLIP
                     WSTTEFAAQEGLTIPAQVNYVGKSLDLYRNGYQFHGSALVIPPYLRGTWLWEKVRVQG
                     GAYGGMCAFDRLSGIFTFFSYRDPNLDQTLTNFDLSSQYLRSLQLSDQELTKAIIGAI
                     GDLDTYQLPDAQGLTALVRYLTGINDEMRQRIRNEVFATTAAHFHQFGEAMAASQAEN
                     KIVILGAQDALTAAGQPLTIKKVL"
     gene            1439..4492
                     /locus_tag="VN363_03005"
     CDS             1439..4492
                     /locus_tag="VN363_03005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559857.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin cofactor-binding domain-containing
                     protein"
                     /protein_id="HWQ83506.1"
                     /translation="MFLNLQINHQSLELEFQPGDSLLKVLRANGYFGVKHGCETGECG
                     ACAVLLDGKPVNSCILLAAQAEGHSIETIEALGEHPEQGWKTTAGLHPLQQAFVETGA
                     IQCGYCTPAQLLAAQSLLEQNPDPAEEEVREALSGVLCRCTGYHKPVQAVLRAAAVLR
                     GEVVPPIRSPEGGEPILAPDSWFAPDGEQPEPLPDHGPASSPVTTRTPVMPRITVSSE
                     NKTWQQVGKPERKVDAVKLVQGKPAFTADFERRGMLIAKVLHSPHAHARIRQIDASAA
                     RALPGVAAVVTWQDIPRIVYSTAGQSDPIPGPLDNFSLDNKVRFVGDRVAFVAAETNE
                     IAEQALSLIVVDYEILPALLDPAQAEAPDAPRLHDEPEYVNFGDSDPSQNLAARIHID
                     IGNIEKGLAEADQVFEAEYEVPKVQQAHIEPHVVITYWDEDDRLVIRTSTQVPFHVRR
                     QLAPVLGLPIKRIRVIKPRIGGGFGGKQEVMIEDIAAHLTIATGRPVYLEYTREQEFI
                     AARSRHPMRIRMVTGVKWDGTITANAMYALSDTGAYGCHALTVTGNTGHKAMALYVGD
                     GAYRQSPNIRFYADVVYTNHPPSGAFRGYGVPQGFWPVERHMEKIARSLGMDPIEFRL
                     KNALRSGELHPFSTAWSEGREPRPETIQTNGLEECVRQGKAAIGWDQKYANPEWRELT
                     GADGQPHPYLRRGIGVAMVMQGTAIPYLDMGGASIKMNEDGSFNLLIGATDLGTGSDT
                     VLAQMAAEVLGVSTEDIVVYSSDTDFTPFDKGAYASSTTYISGTAVVRAAQVCAERIR
                     VRGARLLNERAAPGTAALEANEISLRNRHASAPDGRFVTLAEIGYNAMHHEDQEQIMG
                     VGSYVSPVAPPPFAAQFAEVTMDIQTGQLVVDRLVMAVDSGTIINPITASGQIEGGMT
                     QALGYAVCEEMRYDDAGQARERDFRDYHIFQANEMPRLETIFIETFEPSHPFGVKAVA
                     EIPMDGVAPAVGNAVRDACGIDIDSNPLTPEKIWRALHDKQLAHP"
     gene            4527..>4884
                     /locus_tag="VN363_03010"
     CDS             4527..>4884
                     /locus_tag="VN363_03010"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83507.1"
                     /translation="MSTIYVIGHVNPDTDSIASAMGYAWLLRERDGLDTIAARAGAIN
                     PQTSWVLKNLGIEPPVLVNDASPRFELVARRLATTTPNQPLREAWNIASRTWGVAPVI
                     NEDGSPFGLVNGTSIFT"
BASE COUNT         1123 a         1379 c         1346 g         1036 t
ORIGIN      
        1 gatatgcaga tcctgcaaac ccgccagaat acacctgatt ctccagaagc attagccaca
       61 atacccgtat taaaactggc agatcttgat ccaaaaatca aaaccatccc gactgaaata
      121 cagtcgattt cgggcactcc caccctgatt catgatctgt ttacgaatgg catcgtatac
      181 ctgaacctgg catttgatct ccataccctg cctgcagcct ggctcccttt cattccactc
      241 ttcgggcagg cgctggttga gatgggcacc tcgcgccagg attatgtgcg cctttcccag
      301 cgcatcgggc gggcgaccgg tggtatccat ccggattggc tcaccagcaa cgtagaaaag
      361 agcgcccgga gcgctgcgta tcttttcctg catgccaagg cagtcaccaa ccaggctggt
      421 gagttgttta acattttaaa cgatctgctt ttggacgttc gcctggataa tcgtgagcgc
      481 ttcaaacaaa tcgtgctcga agcaaaggct ggcctggaag caggactggt acctgccgga
      541 cactggttcg tcaaccgccg cctgcgcgcc tcctacacag aagccgattg ggccagcgaa
      601 caaatgggcg gggtcgagta cctgttcttc ctgcgccagc ttatcgatca ggttgaaaat
      661 cactggacca gtgttttgga tactctggaa gggattcgca gccggctggt caaccgccag
      721 gcgatggtag taaatgttac cctggaccaa aaaggctgga gtaaagttca accgaagcta
      781 accgaattct tagagcggct acccgagggc gagcttaatc tgattccctg gagtacgact
      841 gaatttgccg cgcaagaagg cctgaccatc cctgcccagg tcaattatgt tggcaaaagc
      901 cttgatcttt accgcaacgg ttatcaattt catggttcag ccctggtaat ccccccctat
      961 ctccgcggaa cctggttgtg ggaaaaggta cgtgtccagg gcggtgcata cggcggcatg
     1021 tgcgcctttg atcgcctgtc aggtattttc actttctttt cttaccgtga tccgaacctg
     1081 gaccagacac tcacgaactt cgacctgagc agccagtatc tacgctcgct gcaattaagc
     1141 gaccaggaac tgaccaaagc gatcatcggg gcgataggcg acctggacac ctatcaactt
     1201 cccgatgctc agggcctgac tgccctggta cgctatctca ccgggatcaa tgacgaaatg
     1261 cgccagcgga tcaggaatga agtgtttgct acaaccgcag cccatttcca ccaatttgga
     1321 gaggcaatgg cggcatctca agcggaaaat aagattgtta ttctcggcgc tcaggatgcg
     1381 ctgactgcag ctgggcagcc actaaccatc aaaaaagtac tctaacaggg ctataaccat
     1441 gttcttgaat cttcaaatca accaccagtc gctcgagcta gaattccaac ccggcgacag
     1501 cttgctgaaa gtcttgcgcg ccaatggata tttcggcgtc aagcacggct gtgagaccgg
     1561 agaatgcggc gcatgtgcag ttcttctgga tggaaaaccg gtcaattcgt gtatcctcct
     1621 ggcggcacag gccgaaggac actcaatcga gaccatcgag gcattgggag agcaccctga
     1681 gcagggatgg aagaccactg ccggattaca tccattacaa caagcgtttg ttgaaactgg
     1741 cgccatccag tgcggatact gcacgcccgc ccagctgctg gccgcccaga gcctgctgga
     1801 gcagaatccc gatcccgctg aggaagaagt tcgtgaagcg ctttccgggg tgctgtgtcg
     1861 ctgcacaggc tatcacaagc ctgtccaggc ggtcttgcgc gccgcagcag tgctgcgcgg
     1921 cgaagttgtg ccaccgatca ggtctcctga gggtggagaa ccgatcctgg ctccggatag
     1981 ctggttcgca cccgacggcg agcagccgga gccattaccc gatcatggac ctgccagctc
     2041 accggtcacg acccgcacac cggtcatgcc gcgcattact gtgagcagcg aaaacaaaac
     2101 ctggcagcag gtgggcaagc ccgaaagaaa agtcgatgcc gtcaagctgg tgcagggcaa
     2161 gccagccttt accgctgatt ttgaacggcg gggtatgctg atagctaaag tgctgcacag
     2221 cccacatgcc catgcccgca tccggcagat cgatgcttct gccgcccgcg ccctgcccgg
     2281 cgtagcagcc gtagtgacct ggcaggatat cccccggatc gtctattcaa ccgcagggca
     2341 gtccgatccg atccctggac ccctggataa tttctcactc gacaataaag tccgcttcgt
     2401 tggcgacaga gtcgctttcg ttgctgccga aaccaatgaa attgctgaac aagcgctctc
     2461 attgatcgtg gttgattatg aaatcctgcc cgcgctgctc gaccctgctc aggcagaagc
     2521 gcctgacgct ccccgcctgc acgatgagcc ggaatacgtc aactttggcg attccgaccc
     2581 cagtcagaac ctggcggcgc ggatccatat cgatattggc aacatagaaa aagggctggc
     2641 cgaagccgac caggtatttg aagctgagta cgaagttcca aaagtccaac aggcgcatat
     2701 tgaaccccat gtggtgatca cctactggga tgaggatgac cggctggtca tccggaccag
     2761 tacccaggtt ccattccatg tccgcagaca gctggctccc gtattaggcc tgccgattaa
     2821 gcggatccgg gtgattaaac cccggattgg cggtggcttt ggcggcaaac aagaagtcat
     2881 gatcgaagat atcgccgccc acctgaccat cgccaccggg agaccagttt atttagaata
     2941 cacccgcgag caagaattca ttgccgcccg ctcccgccac ccgatgcgca tccgcatggt
     3001 caccggggtt aagtgggatg gcacgatcac tgccaatgcg atgtacgcct tgagcgatac
     3061 cggggcttac ggctgccatg ccctgacggt gaccggtaat accggacaca aagccatggc
     3121 gctgtatgtc ggggatggcg cataccgcca atctcccaat atccgcttct atgcagatgt
     3181 ggtctatacc aaccatcctc catcaggggc ttttcgcggt tatggtgtac cgcagggatt
     3241 ctggcccgtg gaacggcata tggaaaagat cgcccgcagc ctgggtatgg atcccatcga
     3301 attccggctc aaaaatgctt tacggtctgg tgagctgcat cctttcagca ctgcctggag
     3361 tgaaggacgc gagccgcgtc cggagaccat ccaaaccaac ggtctggagg aatgtgtccg
     3421 ccagggcaaa gcggcgattg gttgggacca gaaatacgct aaccctgaat ggcgagagct
     3481 gactggcgcg gatgggcagc ctcaccctta cctgcggcga gggattggcg tggcaatggt
     3541 tatgcagggt accgcgatcc cctatctgga tatgggggga gccagcatca agatgaatga
     3601 ggatggctca ttcaatctct tgattggtgc aacagacctg ggcactggct cggacaccgt
     3661 cctggctcaa atggctgccg aggtgttggg cgtatccact gaagatatcg tggtgtactc
     3721 ttcggatacg gactttaccc cctttgacaa aggcgcgtat gcgtccagca caacttacat
     3781 ttccggtact gcagtggtcc gtgctgcgca ggtctgtgcg gagcgcatcc gggtgcgcgg
     3841 ggcgcggctg ttgaatgagc gggcagcacc aggcactgct gcacttgaag caaacgagat
     3901 aagtctgagg aatcgccacg cctcagctcc ggatgggcgt tttgtaaccc tggctgaaat
     3961 tgggtacaac gccatgcatc acgaagacca ggaacagatc atgggagtgg gctcctatgt
     4021 atctccggtc gctccgccac ctttcgcagc ccagtttgct gaggtaacca tggatattca
     4081 aaccgggcag cttgtcgtgg accggctggt tatggcggtt gactccggga ccatcatcaa
     4141 cccgatcacc gcttcgggac aaattgaggg gggtatgact caggcactgg gatatgcagt
     4201 ctgtgaggaa atgcggtatg atgatgccgg gcaggcccgt gaacgcgatt ttcgagacta
     4261 tcacattttt caggcaaacg agatgcccag gctggagaca atttttatcg agactttcga
     4321 gccatcccat ccatttgggg tgaaagctgt agcagaaatc ccaatggacg gggttgcccc
     4381 cgctgtaggg aacgcggtta gagatgcgtg cggcatagac atcgattcaa acccgctgac
     4441 accggaaaaa atttggcgag cgctgcatga taagcagctt gctcacccat agccaccaac
     4501 ctgcgagcca tggagaattc ctcaatatga gcacgattta cgtgattggt catgttaacc
     4561 cggataccga ttcgatcgca tcggcgatgg ggtatgcctg gttattacgc gagcgcgatg
     4621 gactggacac gattgccgcc cgcgccggag cgatcaatcc tcaaacttcc tgggtgttaa
     4681 aaaaccttgg catagagcct ccggtacttg taaatgatgc ttctccgcga ttcgagctgg
     4741 ttgccaggcg ccttgccacc accaccccga atcaaccgct ccgtgaagcc tggaatatcg
     4801 cttcccgtac ctggggtgtc gcccctgtga tcaatgaaga tgggtcacca ttcggattgg
     4861 taaacggcac gagcatcttt acct
//
LOCUS       DATMXK010000164         2724 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_81205, whole genome shotgun sequence.
ACCESSION   DATMXK010000164 DATMXK010000000
VERSION     DATMXK010000164.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2724)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2724)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2724
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81205"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1177
                     /gene="mgtE"
                     /locus_tag="VN363_03015"
     CDS             <1..1177
                     /gene="mgtE"
                     /locus_tag="VN363_03015"
                     /inference="COORDINATES: protein motif:HMM:TIGR00400.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="magnesium transporter"
                     /protein_id="HWQ83508.1"
                     /translation="TADLLEELDDDDALEAVEDLSAGHLADLLDEMEPDEAADLLGDM
                     PRDQADIVLEQMEDADEVRPLLGYPDETAGGLMTTSFVALRRQTTAEQAIQFLREISP
                     TSEIPYYLYVVDREQKLTGVVGFRELVISSPQTTMQQIMDPDVISVSVGVDQEEVARQ
                     MSHYSLASIPVTDARQRLVGVITHDDILEVIEDEATEDIYRLANVSDTELEPDSPLAD
                     QVRGRLPWLFLNMLTALFASWVVSNFESLIGEIAILAAFQSVVAGLGGNSGSQNIVMM
                     VRALALDRIDKKKIWNILGRQVLVGLLQGVVVGSVVAIVITVLRGDFHLGLILLLAMT
                     ANMVVAAVVGTLVPLFLARIGKDPAVASTILLTAATDSFGFFIFLSLASLFVRFFDI"
     gene            1198..2124
                     /locus_tag="VN363_03020"
     CDS             1198..2124
                     /locus_tag="VN363_03020"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWQ83509.1"
                     /translation="MPAKVILNPYANRWKALERKAELEAALRGAGVEFELAITERPRH
                     GEELAEQAALEGYSPIVSAGGDGSINEILNGIGRGAALTGKPWVPMGVMPLGSANDFV
                     DNLSLPRDLPGAAQVIAAGYTRAMDVCTANGRFFGNNAAIGLEPFITLIQQEIHWLKG
                     TPRYLLATLMGVQRNPQWQMQLEWDGGSYTGRTTLVTVGNNPRTGGLFYMTPHADPFD
                     GKLTFVYAYMRTRSEILKLLPRTMKPGPGSYVEHPAVHEVHTTWLKVHSIEPTPAHTD
                     GEIFDVQAHAIDYQIIPGCLPVLLSPPGTPGV"
     gene            2158..>2724
                     /locus_tag="VN363_03025"
     CDS             2158..>2724
                     /locus_tag="VN363_03025"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF013382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ83510.1"
                     /translation="MNRWLRAFLRLFFRLLYHEFAWSYDLVARVVSLGRWNSWVLSVL
                     PYMEGPRVLELGQGPGHLQIALAEKGISVYGLDASPQMVRLAAWRMHHADRRQSQKAG
                     NPEYLSLQPRLLIGAAPNLPYPSGYFDQIVTTFPTEYIFQQPTLIEIKRILRPGGSLL
                     IIPAAWITGRSWLERLAAGLFHVTGQAPA"
BASE COUNT          545 a          792 c          777 g          610 t
ORIGIN      
        1 gactgcggac ctgcttgaag agctggacga tgatgacgcc ctggaagcgg tagaagatct
       61 gtccgccgga cacctggccg atctgctgga tgagatggag ccagacgaag ccgccgatct
      121 cctgggggac atgccgcgcg atcaggctga catcgtcctg gagcagatgg aagacgccga
      181 cgaagtgcgc ccgctactcg gttatcccga cgagacagcc ggcggcttga tgaccacttc
      241 ttttgtcgcc ctgcgccggc agacaactgc agagcaggcc atccagttcc tgcgcgagat
      301 cagcccgacc agtgaaatcc catattacct gtatgtcgtc gatcgcgaac aaaaattgac
      361 cggggtggtt ggttttcgcg agctggtaat ctccagcccg caaaccacga tgcagcagat
      421 tatggacccg gatgtaatca gcgtgtccgt tggtgttgat caggaagaag tagcccggca
      481 gatgtcgcac tactcgctgg cttccatccc ggtcaccgat gcccgccaac gcctggtcgg
      541 cgttatcacg cacgatgata tcctggaagt aattgaggat gaggcgactg aagatatcta
      601 ccgccttgcc aatgtttccg acaccgagtt ggaacccgac agtccgctgg cagaccaggt
      661 gcggggacgt ttaccctggc tctttctcaa tatgctcacc gcgttatttg cttcgtgggt
      721 tgtgagcaat tttgaatccc tgatcggcga gatcgccatc ctggctgcat tccagtccgt
      781 tgtagccggc ctgggcggta attcgggctc tcagaacatc gttatgatgg tgcgtgccct
      841 ggcattggac cggatcgata aaaagaaaat ctggaatatc ctcgggcgcc aggtgctggt
      901 aggcctcttg cagggtgttg tggtcggctc ggtggtcgcg atcgtcatca ccgtactgcg
      961 gggtgatttc cacctgggct tgattttgct cctggcgatg accgcgaata tggtcgtggc
     1021 agccgtcgtt ggcacactgg tgccgttatt tttagccagg atcggcaaag acccggcagt
     1081 ggcatcgacc atcctgctca cggctgcgac ggacagcttc ggctttttca tctttctatc
     1141 cctcgcttcg ctcttcgtcc gcttttttga tatctaaaac tcatctttgg agaaaccatg
     1201 ccagctaaag tgatcctcaa cccgtacgcc aatcgctgga aagctctgga gcgaaaagct
     1261 gagctggagg ctgctctgcg gggagctggc gttgaatttg agctggcgat caccgaacgt
     1321 ccgcggcacg gggaggagct ggctgaacag gctgccctgg aaggttactc tccaattgtc
     1381 tccgccggcg gggatggcag tatcaacgaa atcctcaatg ggattggccg cggagcggcg
     1441 ctcaccggca agccctgggt tcccatggga gttatgccgc tcggctcggc caacgatttt
     1501 gtggataatc tgagcctgcc gcgggatctg cctggtgcgg cccaggtaat cgcagccggg
     1561 tacacccggg cgatggacgt atgtacagcc aacggccggt tttttggcaa caatgctgcc
     1621 atcggactcg agccattcat caccttaatc cagcaggaaa tccattggct caaagggact
     1681 ccccgctatt tgttggcgac cctgatgggt gttcagcgca atccgcagtg gcaaatgcag
     1741 ctggaatggg acggaggcag ctacacgggc cggacaaccc tggttaccgt tgggaataat
     1801 ccccgcaccg ggggcttgtt ctatatgacc ccacatgccg atccttttga tggaaagctg
     1861 acttttgtct acgcatacat gcgcacccgg tccgagatct tgaagcttct gccgcgcaca
     1921 atgaaacccg gcccaggcag ctatgtggaa catcccgccg ttcacgaagt ccatactacc
     1981 tggctcaagg ttcactccat cgagccgacg ccggcccata ccgatgggga gatctttgat
     2041 gtgcaggcac acgctattga ttaccagatc attcctggct gtttaccggt cctgctctcg
     2101 ccacctggca ccccaggcgt gtaaaccatc tgaccgggtc cataagccag aaaaagcatg
     2161 aatcgctggc tcagggcgtt tttgcgcctc tttttccgcc tgctgtacca cgaattcgcc
     2221 tggtcctacg atctggttgc ccgggtagtt tccctggggc gctggaatag ctgggtcctg
     2281 tcagttttac cctatatgga agggccgcgg gtgctggagc tcggccaggg tcccggacac
     2341 ctgcaaattg ctctggccga gaaaggtatc tcagtgtatg ggttggacgc cagccctcag
     2401 atggtccggc tggctgcctg gcggatgcac cacgccgacc ggcgccagag ccaaaaagct
     2461 ggcaatcctg aatatctctc gctccaaccc cgcctattga ttggcgcagc tcccaatctg
     2521 ccctacccat ccggttattt tgatcaaatc gtcaccacat tcccaaccga gtacatcttt
     2581 cagcagccca cattgatcga gatcaaacgg atattacgcc ccggcggcag cttgctcatc
     2641 attccagcag cctggatcac cgggcgcagc tggttggaac gcctggcggc gggtttgttt
     2701 cacgttaccg ggcaagcacc tgcc
//
LOCUS       DATMXK010000165         2460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_60019, whole genome shotgun sequence.
ACCESSION   DATMXK010000165 DATMXK010000000
VERSION     DATMXK010000165.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60019"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            168..527
                     /locus_tag="VN363_03030"
     CDS             168..527
                     /locus_tag="VN363_03030"
                     /inference="COORDINATES: protein motif:HMM:NF015513.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PadR family transcriptional regulator"
                     /protein_id="HWQ83511.1"
                     /translation="MPNSKQTIPPQSASPLSETTFLILVSLGSGPKHGYAIMKEVETL
                     SQQRVLLSTGTLYGAIKRLLADGCISRLNLPEDTRDGRVRKTYTLTSHGRKVLKDETR
                     RLQELVEVAARRASQGA"
     gene            619..2055
                     /locus_tag="VN363_03035"
     CDS             619..2055
                     /locus_tag="VN363_03035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83512.1"
                     /translation="MHAVFADALSAASQEGLWAMLRLCSRELFSLPHALAREHFQSGW
                     KEEASMDNRLQISAVSPAASPASAGQGPVYAWKDVLLAMLPFGVILLVEVLVRLFVVS
                     GLLARESPIRQPLNIALISLLIITFLLGLVLAWRQKWPVWSAVWYPFFLLVPVILVGW
                     LLNLLFDASDMQDGLYIFIPFTLAVLLYTVTRLNRLRGLLAVLTVLYFFWLPNMESVP
                     EWIQVAIKLPSTILICLTIAYLLRRGDWRSGLFAVLAMNLAVGALHSYAGIYYGGTLP
                     FVAPGPNLVEVLRSLVPQYLAGGAILLGPLFARMVRQAGRSGSSYSRVSYHISLVGLM
                     LVILVMLYALLQGSSSSSELWISLLKDNVHSAVILLGIAMYLVGLFLTYRGARNAAAR
                     PGWVETILLAVLPLAIPLTFALPFITWKWPLTLLYGVPLLWELPHAVSLSIGLVWLVL
                     SVWVVTRETAGPAAAVMVTPAISDPKIL"
     gene            complement(2364..>2460)
                     /locus_tag="VN363_03040"
     CDS             complement(2364..>2460)
                     /locus_tag="VN363_03040"
                     /inference="COORDINATES: protein motif:HMM:NF025432.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF4256 domain-containing protein"
                     /protein_id="HWQ83513.1"
                     /translation="FGDRRYDHVFIYHNGADSYYAARGFRGSLRV"
BASE COUNT          470 a          673 c          713 g          604 t
ORIGIN      
        1 ttccgaaaac cggatgagta tcctctcggg cggacaggag ttccagaagg tgacattgtt
       61 tcgcaggggc aggtaaattt cgttggcaat tcccgcttga catctgccaa tatttccgct
      121 aatatgtagt catacgacat atcaccggat gatataaggg agcgcctgtg cctaattcga
      181 aacaaacaat cccaccccaa tctgcgtccc cactttcgga aactactttt ttgatcctgg
      241 tgagcctggg cagcgggccc aagcacggtt acgcgattat gaaggaagtg gaaacactga
      301 gccagcagcg cgtgctactc agcaccggca cgctgtatgg cgccatcaaa cgcctgctgg
      361 cggacggctg catcagtcgg ctgaacctgc cggaagatac gcgggatggg cgggtgcgca
      421 agacatacac cctcacttct cacgggcgca aggtattgaa ggatgagacc cgccgcctgc
      481 aggagctggt tgaggtggca gcacggcgcg cctcgcaggg agcctgagat gaagctcatt
      541 ggctgggctt tattgcggtt atattacctc cagctacatc tgtatcccag aaagttccgg
      601 tcggatttcg gagccgagat gcatgccgta tttgccgacg cattgtcagc tgccagccag
      661 gagggcttat gggcgatgct gcgcctgtgc agccgtgagc ttttttcgct gccccacgcc
      721 ctggcgcgcg aacactttca atcaggatgg aaagaggagg caagcatgga taatcgcctg
      781 cagatttccg ccgtttcacc agctgcctcg ccggcatctg ccgggcaggg gcctgtgtat
      841 gcatggaaag atgttttgct tgcaatgctg ccctttgggg tgatcctgct ggtagaagtg
      901 ctggtcaggt tgttcgtcgt aagcggtctg cttgcccggg aaagccctat ccggcaacct
      961 ttgaacatcg ctctgatcag cctgctgata atcaccttcc tgctgggact ggtcctggcg
     1021 tggcggcaga aatggccagt ctggtcggcg gtctggtatc cttttttcct gctggtgcca
     1081 gtgatcctgg tgggttggct gttgaacttg ctgttcgacg catcagacat gcaagatggc
     1141 ctgtatattt ttataccatt caccctggca gtgctgttgt ataccgtaac ccgcctcaac
     1201 cggctgcgag gcctgttggc tgtcctgacg gtgctttatt tcttctggct gccgaacatg
     1261 gaatccgttc cggaatggat tcaagtcgcc atcaagctcc ccagcaccat cctgatctgc
     1321 cttacgatcg cttatctgct gcgccgggga gattggcgca gcggtctgtt cgctgtattg
     1381 gccatgaacc tggcagtggg tgccctgcat tcttatgccg gcatttatta cggtgggacg
     1441 ctgccttttg tggcgccggg acctaacctg gttgaggtat tgcgcagcct ggtcccccag
     1501 tacctggcgg ggggcgcgat cctgctcgga ccgctgtttg ccaggatggt ccgccaggcc
     1561 ggacgaagtg gcagcagtta cagccgggtc agctatcata tctcactggt ggggctgatg
     1621 ctggttatcc tggttatgct ttatgcgctc ttgcagggta gtagctcgtc ttcagaatta
     1681 tggatctctt tattaaagga caatgtgcac tccgcggtga tcctcctggg gatcgccatg
     1741 tacctggtcg gtctattcct gacctaccgg ggcgctcgga atgcggctgc aaggcctggc
     1801 tgggtggaaa ccatattgtt agctgtgcta cccctggcga ttccattgac cttcgccttg
     1861 ccttttatca cctggaagtg gccgctcacc ctgttgtacg gcgtcccgct attgtgggag
     1921 ctgcctcacg cggtcagcct gtccatcggg ctggtctggc tggtgctgtc tgtctgggtg
     1981 gtgacgcggg agactgcggg gccagcggcg gcggttatgg tcaccccggc catatccgac
     2041 cctaaaattc tttgagactt ttgaggtttc gcccgatcga tcgcttgatc ccagggacat
     2101 cactcagatc cccatctgcc agcggatcag cttttcgagc agccaggcca gggacacacc
     2161 gccatgtacg gcgcgccgga gctgttcctg aatgaagtta ccggcttttt acttgaataa
     2221 tcaacccggc tgataaatag agaagtcgtc tttctccctt accggaatag actccgggtc
     2281 attatcgttc cgcccatcga agaagcttac ggctatcttg cggggatcgt tacaaatata
     2341 gatcgcgacg agagtgacct ggttcagacc cttagcgagc cgcgaaaacc ccgagcagca
     2401 taatatgaat ctgcgccgtt gtgataaatg aagacatggt cgtagcgccg gtcgccaaag
//
LOCUS       DATMXK010000166         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_33173, whole genome shotgun sequence.
ACCESSION   DATMXK010000166 DATMXK010000000
VERSION     DATMXK010000166.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33173"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..603
                     /locus_tag="VN363_03045"
     CDS             <1..603
                     /locus_tag="VN363_03045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558576.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ83514.1"
                     /translation="MQASVEATLAAFGKIDSLINGAGGNRPAATTSSEKLFFDLPQDA
                     IRAVFDLNLLGTIIPSQLIGRHMAERKQGVILNISSMNSFRPLTLIPAYSAAKAGVSN
                     FTQWLAVHMAQEYSPEIRVNAIAPGFFVTEQNRFLMTDAETGKPTRRGQKVLDHTPMG
                     RYGSPEDLVGTTLWLLSPASAFVTGIVVPIDGGFSAYSGV"
     gene            653..1915
                     /locus_tag="VN363_03050"
     CDS             653..1915
                     /locus_tag="VN363_03050"
                     /inference="COORDINATES: protein motif:HMM:NF021387.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0050043 - lactate racemase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lactate racemase domain-containing protein"
                     /protein_id="HWQ83515.1"
                     /translation="MITAQKISPTGLLEPDSIYATLSLGLAGNFTHQKVLVLIPDYTR
                     SLPMPMLFRMVVDILRDVRQLDFMIALGTHPPMSAEAINHLVGITAAERETTYKHIGL
                     LNHAWDDPTMLASLGVMEQDEIKQIAGSSWHSSLPDRVDIRINCLALEYDQIIILGPT
                     FPHEVVGFSGGAKYLFPGISGPDMINATHWLGALATVVGTIGIKDTPVRAMIHAAAQR
                     LKTPVTLAALIVEGHDLVGVIVGDLLSAWSAAADASAQRHIHWCEKPFQRVLSWAPVM
                     YDELWTAAKAMYKLEPAVAPGGEVIIYAPHLDVVSHVHGKYIFETGYHILPYFLSDWE
                     RFKHVPLGVLAHSTHVRGSGMMENAIEKANVRVTLASKVSPEDCARLNLGYLDPAEIN
                     LADWQDREDEGILYVPRAGEILYRLKTS"
     gene            1925..>2772
                     /locus_tag="VN363_03055"
     CDS             1925..>2772
                     /locus_tag="VN363_03055"
                     /EC_number="2.7.1.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR01313.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="gluconokinase, GntK/IdnK-type"
                     /protein_id="HWQ83516.1"
                     /translation="MFIVVMGVSGSGKSTIARQLAQRLGWTFYDADDFHSPENIARMA
                     AGIPLTDEDRAGWLDSLAALVEDQTQAGKDGVIACSAMKKEYRDVLRRYDRKQIQFVY
                     LKGSYEVIHARLQRRGEHFMKPEMLQSQFAALEEPWGILTIDVTLPQSEIVQIIMEQL
                     MDTKYALGILGLGVMGRSLAQNFARNGYPPIGYDLAPRLPDDFDVKVVNSLDELVAAL
                     TPPRTLFLMVPAGAPVDAAIASLKPYLQQGDLIIDGGNSYFADTERRVKELEQAGLQF
                     IGMGVSG"
BASE COUNT          585 a          794 c          723 g          670 t
ORIGIN      
        1 atgcaagcct cagttgaggc aactttggcc gcctttggaa aaatcgacag cctgatcaat
       61 ggcgccggtg gcaaccgtcc ggctgccact accagttccg aaaaactatt ttttgatctg
      121 ccccaggatg ccattcgcgc tgtctttgat ttgaacctgt tgggcacgat catcccctct
      181 cagttgattg gtcgccatat ggccgagcgt aaacaagggg tgatcctgaa tatttcttcc
      241 atgaactcct tccgccccct gacccttatt ccggcttatt cggcagccaa ggctggagtc
      301 agcaatttta cccaatggct cgcagttcac atggcccagg agtactcacc tgagattcgt
      361 gtcaacgcca ttgcacccgg tttcttcgtg actgagcaga atcgttttct catgaccgac
      421 gccgaaaccg gcaagcccac caggcgcggt cagaaggtcc tcgaccatac cccgatgggt
      481 cgctatggtt ctcccgaaga tctcgtcggc accacgctct ggctgctttc cccggcttca
      541 gccttcgtca ccgggattgt cgtcccgatc gacggtggtt tttcagccta cagcggcgtt
      601 tagctggccc ccaggctcat tttcttccta tcccttcagt cgaggaaaat caatgatcac
      661 tgcccaaaaa atctcaccca ccggtttgtt ggagcctgat tcaatctacg caaccctctc
      721 gctcgggctg gcgggaaatt ttacccatca aaaagtattg gttctcatcc ccgactatac
      781 ccgctcgctg cccatgccca tgctcttccg gatggttgtt gacatcctga gggatgtgcg
      841 ccagctcgat tttatgatcg ccctcgggac gcatccgcca atgtccgctg aggctatcaa
      901 tcacctggtc gggattacag ccgctgagcg ggagacaacc tataaacaca ttggccttct
      961 caatcacgcc tgggatgatc ccaccatgtt ggcttccctg ggggttatgg aacaggacga
     1021 aatcaaacaa atcgccggat cgagctggca ctcctctctg cccgaccggg ttgatatccg
     1081 catcaactgc ctggcgttgg aatacgacca gatcattatc ctcgggccca ccttcccgca
     1141 tgaagtcgtc ggcttttctg gtggagcaaa atacctgttc cccggcatct ctggtccgga
     1201 tatgatcaat gccactcact ggctgggcgc cctggcgaca gtggtgggta ccatcggcat
     1261 caaagatacg cctgtgcggg ccatgatcca tgccgccgca cagcgcttga aaacgccggt
     1321 aaccctggcc gcccttatcg tggaaggtca cgatctggtg ggcgtgatcg ttggggatct
     1381 gctgtctgcc tggagtgcag ctgcggatgc ctcagcccag cgccatattc attggtgcga
     1441 aaagcccttc caacgtgtcc tgtcttgggc tccagtcatg tacgatgagc tgtggacggc
     1501 tgccaaagcc atgtacaaac tcgagccagc cgttgctccg ggtggggaag tcatcattta
     1561 cgctccccat ctggatgtcg tcagccatgt gcacggcaag tatatcttcg agacaggcta
     1621 tcacatcctc ccttattttc tgagcgactg ggagcgtttt aagcatgtcc ctctgggtgt
     1681 tttagcccac agtacccatg tccggggctc tggcatgatg gaaaacgcca tcgagaaagc
     1741 caatgtccgg gttaccttag ccagtaaagt ctcgcctgaa gactgtgccc gcctgaacct
     1801 cggctacctc gatcctgccg agatcaattt agcggattgg caggaccggg aagacgaagg
     1861 gatcttatat gttccccgcg ccggtgaaat cctttaccgg ttgaaaacca gttgagaatt
     1921 ggcgatgttt atcgtcgtga tgggtgtatc cggtagtggc aagagcacca tcgctcgcca
     1981 gctcgcccaa cgcttgggct ggaccttcta tgatgccgat gattttcatt caccggaaaa
     2041 tattgccaga atggctgccg ggattcccct cacagacgaa gatcgcgccg gttggttgga
     2101 ttcattggct gcattggttg aggatcagac ccaggccggt aaagatgggg taattgcatg
     2161 ctcagcgatg aagaaagagt accgcgatgt cctgagaagg tacgatcgca agcaaattca
     2221 atttgtatat ctcaagggca gttacgaagt gattcatgcg cgtctgcagc gccgtgggga
     2281 gcattttatg aagccagaaa tgctccagag tcagttcgcc gctttagaag aaccctgggg
     2341 gattctgact attgatgtca ccctcccaca gtcagagatt gttcaaataa ttatggagca
     2401 gcttatggac acaaaatatg cgcttggaat acttggtttg ggcgtcatgg ggcgaagcct
     2461 ggcacaaaat tttgcccgca atggctaccc gcccatcggt tacgatctgg ccccccgcct
     2521 gccggacgat tttgacgtca aagttgttaa ttccttggac gagctggtcg ccgccctcac
     2581 tccacctcgc accctctttc tgatggtgcc cgctggcgct ccggtggatg cagcgattgc
     2641 ttccctcaaa ccctatctcc agcaaggaga tctgatcatc gacgggggta attcctactt
     2701 cgccgatact gaacggcgcg tcaaggagct ggaacaagcc ggtctccaat ttatcggcat
     2761 gggtgtctcg gg
//
LOCUS       DATMXK010000167         3810 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_4806, whole genome shotgun sequence.
ACCESSION   DATMXK010000167 DATMXK010000000
VERSION     DATMXK010000167.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3810)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3810)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3810
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4806"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            213..638
                     /locus_tag="VN363_03060"
     CDS             213..638
                     /locus_tag="VN363_03060"
                     /inference="COORDINATES: protein motif:HMM:NF024773.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0120225 - coenzyme A binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA-binding protein"
                     /protein_id="HWQ83517.1"
                     /translation="MNDTNYASEQEAIKLILQDTRTIAVVGLSASPERAGYYVPAYLQ
                     EAGYRIIPVNPNLTEVLGEKAYPDLLSVPETVDLVLIFQRSEKVLPFVEQAIAIGARA
                     VWMQQGIINQAAAEAARQAGLLVVMDTCMMVEHRKRDGF"
     gene            complement(662..1966)
                     /locus_tag="VN363_03065"
     CDS             complement(662..1966)
                     /locus_tag="VN363_03065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560311.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWQ83518.1"
                     /translation="MQIAIIGAGVGGMAAAFDLVRAGHQVTIYEASQQAGGLATGFKE
                     PHWDWSVEKFYHHWFASDKHMLGLIDELGWREQVIFPRPYTVMYYEGKFYPFDSIPAA
                     IMFPGLGWGIDKIRFGLVGLYLRLTNNWRGLESVTVDEWMRKWAGDRVFELMWEPLLV
                     GKFSDRYARQVNMAWMWARLKARTTRLGTFQGGFQAFNDLFAAKLQDMGVHIEFSTPV
                     QAITHTPDNKLQLITGQGSQIFDQALVTTSPALLARMAPELPPDYLRGLLELKSMGAV
                     VMVLALNQRLSDKGYYWYNIPKSAGFPFLAVVEHTNFLSPEYFGGDHILYIGDYLDKD
                     HPYFQMTADELLDTYTAALPRINPAFNRDWVRKSWLFRTDYAQPVPLVNHSKNIPAIP
                     TPLPGLYFASMSQVYPWDRGTNFAVEIGRKAARMMLSDQAGT"
     gene            complement(1966..2979)
                     /locus_tag="VN363_03070"
     CDS             complement(1966..2979)
                     /locus_tag="VN363_03070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560312.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWQ83519.1"
                     /translation="MKRWQFWVGVLISLGFLYIVLKDLEFGAFVDTLKTANYIWLIPG
                     IGVYFIGVWARAWRWHYLLRPLKSIPTKTMFPIVTIGYMGNNIYPARAGEVLRAVILK
                     RREGVPVSASLATIIVERIFDGVVMLGFVFISLGELSRLTGGSGVVGQVSIQDIAVWG
                     SIAFLGALVIFLAAAMFPDRTLAIIEWLSSHLLPERFRQPVTQVSHRFLAGLASLRSP
                     RDVLMVFVTSVVIWLLETGKYWFVMQAFPFEVSFFTLMLMNGIVNLATTIPSAPGYIG
                     TFDAPGIAVLEAYGVSKALATGYTLVLHVALWLPITLLGAYYLAREGIHWSKDLEKVR
                     GEA"
     gene            3076..>3810
                     /locus_tag="VN363_03075"
     CDS             3076..>3810
                     /locus_tag="VN363_03075"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWQ83520.1"
                     /translation="MQSMKRVRITIIMVLLVLIPAAGITYLLAVPRLTAVSPQANAED
                     IAALSPLQLEFSRRMDADSVTERLHIQPAPRGEIAWDTSGRVLTITPTEPWPSGETIQ
                     VRLETAANASGFLPLTIRQEASWSFTIGQPNLVYLSPSDGPPNLYLLNPFDGNIRQLT
                     NHQEGVTDFDVDSHGTLAYYSVEVPDGSMLYAIEDLASPADQEVAIPEPVLALNCQQA
                     HCSSPRLAPDKSYLAYERTAFTSPGEH"
BASE COUNT          953 a         1090 c          967 g          800 t
ORIGIN      
        1 cagccgggtg tttacggctc aaggaagtga agtggcgtaa aaaatcttcc cggcgaggcg
       61 agcgacccat cgcttctgcc agaattatct ggccgcgggg agataataat tggcggaacg
      121 catctgtatc cattttccag ctattcggtc tgatttcatc gaggacattc atccggattt
      181 gaccggttac agaggcaggt tataatagca gcatgaatga cacaaattat gcctctgaac
      241 aggaggccat caaactgatt ttacaggata cccggacgat tgctgtagtc gggttatccg
      301 caagtccaga gcgagccggt tattacgtgc cggcttatct ccaggaagcc ggctaccgga
      361 tcataccagt aaaccctaat ctgacggagg tccttggcga aaaggcatat cctgatttat
      421 taagtgtacc ggaaaccgtg gatctggttt taatcttcca acgcagcgag aaagtgctgc
      481 cttttgtgga acaggcaatc gcgatcggag cgcgggcagt ctggatgcag caaggaatta
      541 ttaatcaggc tgctgccgag gctgcccgcc aggcgggctt attggtggtg atggatacct
      601 gtatgatggt tgagcatcgt aagcgcgacg gtttttaagg cgcggctcag aaccagcccg
      661 gttaagtacc tgcctggtct gacagcatca tgcgggcagc cttgcgccca atttccacag
      721 cgaagttagt gccccggtcc cagggataaa cctggctcat gctggcaaaa tacaggcctg
      781 gaaggggagt cgggatcgcc gggatatttt tcgagtggtt aaccaacgga acgggttgag
      841 catagtcagt acggaacaac cagcttttcc gcacccaatc ccggttgaat gccggattaa
      901 tacgcggcag cgcggcagta taggtatcca gcagctcatc agcggtcatt tgaaaatagg
      961 ggtggtcctt atccagataa tcgccaatat agaggatatg atccccccca aagtattccg
     1021 gactgaggaa attggtatgc tcaaccacag ccaggaaggg aaagcccgcc gatttcggaa
     1081 tgttatacca gtagtaacct ttgtccgata agcgctgatt aagagccagg accatgacca
     1141 cagcccccat gctctttaat tccaacaagc ctcgcaggta gtctggaggc agttcgggcg
     1201 ccatacgcgc cagtaaagcc ggggaagtcg taaccagcgc ctggtcgaag atctggctgc
     1261 cttgaccggt tattaactgg agcttattat ccggggtatg ggtaatagcc tgcaccgggg
     1321 tggagaattc gatgtgcact cccatatcct gcagcttagc cgcgaacaga tcgttaaatg
     1381 cctgaaaacc gccctgaaat gtacccaggc gggttgtgcg cgcctttaaa cgcgcccaca
     1441 tccaggccat attaacctgt ctggcatatc gatcgctaaa cttcccgacc aataagggct
     1501 cccacatcag ttcgaaaacc cggtcgccag cccatttgcg catccattca tccacggtta
     1561 ccgattccag ccctcgccag ttattggtca ggcgcaggta cagccccacc agaccaaagc
     1621 ggatcttgtc gatcccccag cccaaaccgg ggaacatgat tgctgccggg atcgaatcga
     1681 aaggataaaa tttcccttcg tagtacatca cagtataagg acgtgggaaa ataacctgct
     1741 cgcgccagcc cagctcgtcg atcaaaccca acatgtgttt atcgctggca aaccaatgat
     1801 ggtagaattt ttcgaccgac cagtcccaat gcggctcttt aaaaccagtc gccagacccc
     1861 ccgcctgctg gctggcttcg taaattgtaa cttgatgacc ggcgcgcacc agatcgaatg
     1921 ccgccgccat tcccccaacg cctgctccaa tgatcgcaat ctgcattatg cctcgccccg
     1981 cactttctcc aggtctttac tccagtggat cccctcacgc gccaaataat aagcgcccag
     2041 caaagtgatc ggcagccaca aagcaacatg taaaaccagg gtgtagcctg ttgccagggc
     2101 tttgctaacc ccatacgcct cgagcactgc gatccctggg gcatcaaagg taccaatata
     2161 gcccggtgct gaagggatgg tcgttgccag attaacaatt ccattcatca gcatcaatgt
     2221 gaagaaactc acctcaaacg gaaaagcctg catgacaaac cagtatttgc cagtctccag
     2281 tagccagata accacggagg tcacgaaaac catcaagaca tcccgaggcg agcgcaggga
     2341 agccaggcca gctaaaaagc ggtgcgaaac ctgcgtaacc ggctggcgaa accgctcggg
     2401 gagcagatgg ctggaaagcc attcgataat cgccagtgtc cggtctggga acatggctgc
     2461 cgccaaaaag atcaccagcg ctcccaaaaa cgcgatgctg ccccataccg caatgtcttg
     2521 aatagaaact tgacccacca ctcccgaacc accggtcagg cggctaagct cacctaggct
     2581 gataaataca aaacccagca tcaccacgcc atcaaagatc cgctcaacaa tgatcgtcgc
     2641 cagggacgct gagaccggca caccttcgcg gcgtttaagg attactgcgc gcagcacttc
     2701 cccggcgcgg gcagggtaga tgttattgcc catataaccg atggtcacga tagggaacat
     2761 ggttttggtg gggatagatt tcaagggacg cagcaggtag tgccagcgcc aggcacgtgc
     2821 ccaaactccg atgaagtaca cacctattcc cggaataagc cagatgtagt tggcggtctt
     2881 cagggtgtcg acgaatgcgc caaactcaag atctttgagg acaatatata aaaagccgag
     2941 gctgatcaga accccgaccc agaactgcca tcgtttcata gcttccattc taccacgtcc
     3001 accagtgaag gcttaccctc tctgctttgc accatagggc tctgtttctt taaaaggcaa
     3061 gctggcacgg ttataatgca gtctatgaag cgagtgcgca ttaccatcat aatggtcctc
     3121 ctggtgctca tacccgctgc agggatcact tatctactgg ctgtaccccg cctgaccgca
     3181 gttagccccc aggcgaatgc agaggatatt gccgccctta gcccacttca gctggagttc
     3241 tcccggcgga tggatgcaga ctccgtgacc gagcgattgc atattcagcc agcccctcgg
     3301 ggtgagatag cctgggatac gagcggtcgc gttctgacca tcactcccac cgaaccctgg
     3361 ccgagtggag aaaccatcca ggtccgcctt gagacggcag ccaatgcgag tggttttctg
     3421 cctctcacca tccgccagga agcatcctgg tctttcacca tcggccaacc taatctcgtt
     3481 tacttatccc catcggatgg accgcccaat ctatacctgc tcaatccttt cgatgggaac
     3541 atacgccagc tgaccaatca ccaggaaggc gtgacagact ttgatgtcga cagccacggt
     3601 actctggctt attacagcgt ggaagtgcct gatggcagta tgctctatgc aatcgaagac
     3661 cttgcttccc ctgctgatca ggaggtggcg ataccggaac cagtcctggc gctaaactgc
     3721 cagcaggctc actgcagctc accccgcctc gccccggata aaagctacct ggcctacgag
     3781 cggactgcct tcacctcgcc aggtgaacac
//
LOCUS       DATMXK010000168         7366 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161192, whole genome shotgun sequence.
ACCESSION   DATMXK010000168 DATMXK010000000
VERSION     DATMXK010000168.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7366)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7366)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7366
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161192"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(139..741)
                     /locus_tag="VN363_03080"
     CDS             complement(139..741)
                     /locus_tag="VN363_03080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83521.1"
                     /translation="MAWRLEWVSGEVDRLWPEGVPQIRNEHGSAYVTGYAHDEAGNLV
                     YLGMVGHKTVLESIRATVQARQRKKLFLQGRPIYPLPTHYCQTWQHLPDYGAHHAALI
                     ADPALPGKWQPGEEVIYLLVLEGEFPDELLPETQVRRVLTPRLSETLPIPILDGWHES
                     LWEAGQTEDLIVGLLAGGDCQQGYQVQLTETVWKEVITRL"
     gene            complement(645..1211)
                     /locus_tag="VN363_03085"
     CDS             complement(645..1211)
                     /locus_tag="VN363_03085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83522.1"
                     /translation="MTGKSLPGLTAPFENTYFRERDIKVAARQKQGHILVSGAKPDNG
                     QGLPLPYIHDVPDLRRGNYPYDYECEWGRFKYEYELGSCLFTPHNGQVPPGWERYDLQ
                     APIQPVTAVIDRARRLATVKAPDREIVLRDVPVGESPTTCSSRSTQPWRKAASPSWPG
                     GWSGSAARLTGFGRRASRRSVMNTARPT"
     gene            complement(1221..1745)
                     /locus_tag="VN363_03090"
     CDS             complement(1221..1745)
                     /locus_tag="VN363_03090"
                     /inference="COORDINATES: protein motif:HMM:NF042459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HWQ83523.1"
                     /translation="MDTLDHLVIGRGRFVSLKERGLGFDSRVSETRPGYRVRSTRYSI
                     HEDDDLIYAYTRSQALEDGVLVDVSQMASEADFRYPTAITTDLNARITPNEREKALGQ
                     SYAGRLWDVVFLASFAARRSGINDRCSFDVSLFEADDAPPHRTHRRTLSLWMVVGPGD
                     QGEPVITIGFPEDF"
     gene            complement(1787..2608)
                     /locus_tag="VN363_03095"
     CDS             complement(1787..2608)
                     /locus_tag="VN363_03095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83524.1"
                     /translation="MFGCPPHLVPKWIREAEEVIPGAKGRELRRIGKGEDGEINDVRR
                     FFEDFDAGRLGPKAIAMVASTSAKMGAGWQPVVRAKPIKGKGKAQVIYTCPQCGQVQV
                     DPQGIPITEIDVFQKHRSFCNAQVSGWELDRDGRRKLDKDGNPVWGTRPCAAPLFEYT
                     GVRRWSIAEYIKDKASGRFQILVGDEVHEYKAKASDRGVAFHQLVEASRYTLTLTGTY
                     FGGKSTSIFWLLHRLNPAVRSDFAFHDELRWAGAYGMLESVRNPRPATRRPARTM"
     gene            2652..2930
                     /locus_tag="VN363_03100"
     CDS             2652..2930
                     /locus_tag="VN363_03100"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83525.1"
                     /translation="MASFPAAAQAVHQAVTYFTNNQHRMEYARVRQLGYFIGSGTVES
                     GGKQIATLRLKRAGARWTECGAVSTAKARAAWLSGHWDDLADRRATLP"
     gene            3054..3818
                     /locus_tag="VN363_03105"
     CDS             3054..3818
                     /locus_tag="VN363_03105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83526.1"
                     /translation="MTKVRLLPGDYLELLASIRVFIILMVLSLNQINIIQPEASQVLP
                     DLPALSRATGRFRKGPGSSIELARETTLVEASTLIKPAAIWAKIEISQNDSSKFPVWL
                     AKKASRVESVAGIVCTIGDQLENMSRKYFSVGQNTRGYILDQVGSLAVARLASQVAER
                     LRRELNALYWAPGDDTTDVTLSAQRWLFDLIPAQEIGVRLTGQNVMVPVKSLSFFLLI
                     RTGQTGLKCAIPCQRCVWNGACDQRLQKTSSGNSTA"
     gene            3855..5156
                     /locus_tag="VN363_03110"
     CDS             3855..5156
                     /locus_tag="VN363_03110"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HWQ83527.1"
                     /translation="METTGDHYQEQTDLGADLLKWVEHEHIGQLRTYNRGRMLFWQGD
                     PVEFVYMVKNGAVKVNSISQDGKSYAYGVIGAGGIVGVTSLLQCIAYEFMAEAIEDTQ
                     VIAIAAAEFNQLLLTERQFSLLVIKKLAQGLSLLASKARDSGLLDAQQRLKHRLVELA
                     KDHGSATEKGVKINLDLTHEEIATLVSANRSTITGLLNELKEQGFLWKEGRHLVIIPP
                     VHIEILDELTRSVVNGEEEEAKKWANKAVESGVDPVKALNALTSGMNLNDRMFNRDEI
                     DVSDVILSAYAMKSAIPIVEAEIEKGRQKVHSIGRVVIGTVSGDIHDIGRTLVAMLLR
                     ARGFEVIDLGINVSCDDFTQATLKFKPDILAMSSLMTTSAQEQFKVIRALREAGLREE
                     VKVIVGGGAITEKLKQEMGADGYEPTAHRAVELAWRLTHSA"
     gene            5282..7102
                     /locus_tag="VN363_03115"
     CDS             5282..7102
                     /locus_tag="VN363_03115"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HWQ83528.1"
                     /translation="MAYTIEFEPIGIRLICDDPLTISEAARQAGVSIRSVCGGKASCG
                     KCLVRVMEGHLDSPTEAEQKHLTLAQISAGWRLACRTIIATDTHVYIPANSLTESQVI
                     QIEGAETAAVPDPAVRLLPLLANPPSLSDQIADLERVANALRQQHALESVWAGLPALC
                     ALRSVLRETDWQIEVALRGTEIIAAYPLGQHRPVGMAVDVGTTKLACYLVDLETGRTL
                     AAKGVMNPQIAYGEDVMSRLEAVLVEPGNADRLQAEVVSSINDVAKELCAAQSLTPDH
                     ILDYCLVGNTAMHHLLAKLPVRPLAVSPFIPTLSDVLDIEADRLGLNAAPGARAYLPA
                     PLAGFVGSDHLAFLLASGFDEGDRIRLGIDIGTNTEIALQVGRRIITCSTASGPAFEG
                     AHIHHGMRAAPGAIERVKITEEGGIQCDVIGGVPAVGICGSGILDALAEMYRARIINE
                     RGRFQLNAPGVEMANDDSLIFSLSKGVNGYRDVTLSQHDVDQVMLAKGAIRAGIEILL
                     SKADVAPADIEEIVIAGAFGTYLDPCNAVRIGLIPGVPVERIRAVGNAAGAGARLLLS
                     SLEYRRRAHALAKRIEYVELTIVPGFNKFFAKGVRFPTPA"
     gene            7178..>7366
                     /locus_tag="VN363_03120"
     CDS             7178..>7366
                     /locus_tag="VN363_03120"
                     /inference="COORDINATES: protein motif:HMM:NF014646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="B12-binding domain-containing protein"
                     /protein_id="HWQ83529.1"
                     /translation="MSEPQFDAISKAVIEGEDVEAGRLVEQALEAGLAPLDILQKGVV
                     HGIARAGELWKANEYFLPD"
BASE COUNT         1670 a         1998 c         2076 g         1622 t
ORIGIN      
        1 gattagtcgt aagggcttgt gattgataac gcacaatttt gtggtccgtg taaaccataa
       61 cagggtagga tgctaatcca tcccgtcctg ttatggtttt tctcaccgat cgccggaaat
      121 gcgaagcttt cgttccttct ataacctggt aatcacctcc ttccataccg tctccgtcaa
      181 ctgtacctga tatccctgct ggcaatcacc accagctagc agcccaacga tcaagtcctc
      241 cgtttgccca gcctcccaga gagattcatg ccatccgtcc agaattggga tcggcaatgt
      301 ctctgaaagg cgaggagtga gaacgcgtcg aacctgggtt tccggtaaca attcatctgg
      361 gaattcacct tccaaaacca gcaagtaaat cacctcttcc cctggctgcc acttacccgg
      421 tagggcaggg tcagcgatca gtgcggcatg atgtgctcca tagtccggca ggtgctgcca
      481 ggtctggcag tagtgggtgg gcagcgggta gattggcctc ccttgaagga agagcttctt
      541 gcgctgcctg gcctgaaccg ttgcccggat cgactccagc accgtcttgt gcccgaccat
      601 gcccaggtag accaggttgc cggcctcatc gtgggcgtaa ccggttacgt aggccgagcc
      661 gtgttcatta cggatctgcg ggacgccctc cggccaaagc cggtcaacct cgccgctgac
      721 ccactccagc cgccaggcca cgaagggctg gcagctttgc gccagggctg cgttgacctg
      781 ctggagcagg ttgtaggact ttcgcccacc ggaacgtcgc gcagaacgat ctcccgatcc
      841 ggggctttga cggtcgccag gcggcgcgcc ctgtcgatca cagcggtcac cggctggatg
      901 ggtgcttgca gatcataacg ttcccagccg ggtggaacct gaccattgtg tggtgtgaag
      961 agacaggagc ccaactcgta ctcatacttg aaacggcccc attcacactc gtaatcgtag
     1021 gggtaattgc cacgcctcag gtcggggaca tcgtggatgt acggcaaggg caagccctga
     1081 ccgttgtccg gctttgcgcc agagaccagg atgtgtcctt gcttctgccg ggcagcaacc
     1141 ttaatgtcgc gctcccggaa gtatgtgttc tcaaacgggg cggtcaagcc tgggagtgat
     1201 tttccagtca tggctgcctc ctagaaatct tcagggaaac cgatcgtgat gaccggctcg
     1261 ccctgatcgc cgggaccgac caccatccac aaggagagcg tgcggcggtg ggtgcgatgc
     1321 ggaggcgcat cgtcagcttc aaacagacta acatcgaagc tgcatcgatc gtttatacct
     1381 gatcggcgag cggcgaagga tgccagaaaa accacatccc acaaccggcc tgcgtagctc
     1441 tgccccagcg ctttctcgcg ctcattcggg gtgatgcggg cgttcaagtc agtggttatg
     1501 gcggttggat agcgaaagtc ggcttccgag gccatttgag agacatcgac aagaacgcca
     1561 tcttccaaag cctgggagcg agtgtaagcg taaatcagat catcatcttc atggatggaa
     1621 tacctggttg agcgtacgcg atagcccggt ctcgtctcag aaacgcgact gtcaaacccg
     1681 agcccacgct ctttgagcga aacgaagcga ccacgaccga tgaccaggtg atccagcgtg
     1741 tcgatatcca atagcttgcc agcttgaacg acagcacggg ttacagctac atcgtccggg
     1801 ctgggcgtcg ggtcgccgga cggggattac gcacactttc cagcatgccg tatgccccag
     1861 cccagcgcag ctcgtcatga aaggcgaagt cgctgcgcac agctgggttc aggcggtgga
     1921 gcagccagaa aatcgaggtg ctcttgccgc cgaagtatgt accggtcagg gttagcgtgt
     1981 agcggctggc ttcaacaagt tggtggaacg ccacgccccg gtcgctggct ttcgccttgt
     2041 actcatgaac ctcgtctcca accaggatct ggaagcgccc gctggcttta tccttgatgt
     2101 attctgcgat tgaccagcgg cgcacaccgg tgtactcaaa gagcggcgcg gcgcaggggc
     2161 gtgtgcccca tacggggttg ccgtccttat ccagcttgcg acgaccatcc cggtccagct
     2221 cccagccaga tacttgtgcg ttacagaagc tgcgatgttt ctggaagaca tcaatctcgg
     2281 tgatcgggat gccctgcgga tcgacctgca cctgaccgca ctgtgggcaa gtgtagatga
     2341 cctgggcctt gcctttcccc ttgattggct ttgctctgac caccggctgc cagccagcac
     2401 ccatcttggc cgaggtagag gcgaccatgg cgatagcctt gggacctaac ctaccggcat
     2461 cgaaatcttc gaaaaaccgg cgtacgtcgt tgatctcgcc gtcctcgccc ttgccgatgc
     2521 ggcgcagctc gcgccctttg gctccgggga tgacttcttc cgcctcgcgg atccacttag
     2581 gaaccaggtg cggcggacat ccaaacacct gaccagtttg gcgacttgtt ctgggccacg
     2641 gctgtcaatt attggcttcc ttccctgcgg ctgcacaggc cgtacatcag gctgtgactt
     2701 acttcaccaa caatcagcac cgcatggagt atgctcgcgt tcgccaactt ggctatttta
     2761 tcggcagtgg tacggttgaa agtggtggca aacaaattgc cactctccgt ctcaaacggg
     2821 ctggcgctcg ctggacggaa tgtggcgctg tttcaaccgc caaagccaga gctgcctggt
     2881 tgagcggtca ttgggatgac ttggctgacc gccgagccac tttaccttag gcggtctaac
     2941 aacttttgag tgctccctct ataggttaaa agggttatca cgtctaaaac acatggaaca
     3001 ccgccaaagg aaaaacatca aaaaaaggca gctgtcatca atgtcggata taattgacaa
     3061 aagttcgctt gttgccaggc gattatcttg aattgctcgc aagcatccgc gtgtttatta
     3121 tcttaatggt gctttccttg aaccagatca acataatcca accagaagcg tcgcaagtct
     3181 tgcctgattt acccgcactc agtcgggcca ccggtcgttt tagaaaagga cctggttcat
     3241 ctatagaatt agcccgagaa acgaccctgg tggaggcctc gacattaatc aagccagcgg
     3301 caatttgggc gaaaatcgag atttcgcaga atgattcttc caaatttcct gtttggctgg
     3361 caaaaaaggc ttccagggtt gagtcggtcg ctggcattgt ctgcacgata ggcgatcaac
     3421 tggagaacat gtctcgtaaa tacttctcgg tggggcaaaa cacccgcggt tatattttgg
     3481 atcaggtcgg gtccctggcc gttgcgcgcc tcgccagcca ggttgctgaa cgactgcgcc
     3541 gcgaattgaa tgctctctat tgggcgcctg gcgatgacac gaccgacgtc acgctctccg
     3601 cccagcgttg gctcttcgac ctcattcccg cccaggagat cggcgtccgg cttaccggtc
     3661 agaatgtgat ggtgccggtc aagtcattat ctttttttct gctcatccgg accggacaaa
     3721 ccggtttgaa gtgtgcaatt ccctgccagc gatgcgtctg gaatggcgcg tgtgatcaac
     3781 gcttgcagaa aacttcttct ggcaattcaa cggcatgaaa gaggcggatt gacggcctga
     3841 aggtaagggt tcatatggaa accactggcg atcattatca agagcaaaca gacctggggg
     3901 ctgatctctt gaagtgggtc gaacacgagc atatcggcca actgcgaaca tacaataggg
     3961 gtcggatgct cttttggcag ggtgatcctg tagagttcgt atatatggta aaaaatggag
     4021 ccgttaaagt taattcaatc tcgcaggatg ggaaaagtta tgcttacggg gtcatcggag
     4081 caggaggcat agtcggggtt acctcgttat tacaatgcat cgcatacgag tttatggcag
     4141 aagccatcga agacacccag gtcatcgcca tagcggcagc agaattcaat caattgctgt
     4201 tgacagagcg ccaattttct ctgcttgtca taaagaaact ggcgcaggga ttgagcctgc
     4261 ttgccagcaa agccagagat tcgggcttat tggatgcaca acaacgcctg aaacaccgcc
     4321 tggttgaact ggcgaaggat catggaagtg ctacagagaa gggggtcaaa atcaacctgg
     4381 atctgactca cgaagaaatc gcaacccttg tatctgccaa tcgaagcaca attactggcc
     4441 tcttgaacga gctcaaagag caaggattcc tctggaaaga aggccggcat ctggtgatta
     4501 tcccaccagt tcacattgag atcctcgatg agcttacccg atcggttgtg aatggtgaag
     4561 aggaagaagc caaaaaatgg gccaacaaag ccgtcgagtc aggcgtcgat ccggtaaaag
     4621 cgctcaacgc gttaaccagc ggtatgaacc tgaacgacag aatgttcaac cgggatgaga
     4681 tcgatgtatc cgacgtaatt ctctccgctt atgcaatgaa aagcgccatt cccatcgttg
     4741 aagcggagat tgaaaaaggc agacaaaagg tgcattcgat cggcagggtc gtcatcggaa
     4801 ctgtctctgg agacattcac gatatcggtc gcacattggt tgcgatgttg ctgagagcca
     4861 gggggttcga ggtgatcgat ctcgggatca acgtgagctg cgacgatttt actcaagcta
     4921 ccctaaaatt caaaccagat atattggcga tgtcatctct gatgacgacc agtgcccagg
     4981 aacagtttaa agttatccgt gcattaaggg aggctggttt gcgcgaagag gtcaaagtca
     5041 tcgtcggggg aggcgcgatc acggagaaat tgaaacaaga aatgggcgca gatggatatg
     5101 agcctacagc acatcgcgct gtagagctgg catggagact aacccactcg gcgtagaaga
     5161 acgtctcgaa attgttgttt agccaacaga cagggggagt aatctttgct atccttgatt
     5221 gtagaatatt ctaataccca aaaggatagt gtgaagagtt atcacgctaa tctgcacttc
     5281 catggcctac acgattgaat ttgagccaat cggcatccgt ctgatttgtg atgatccttt
     5341 gacgatctct gaagcagctc gccaggcagg cgtaagcatc cgttcggtct gcggcggcaa
     5401 ggcttcctgt ggcaagtgtc tggttcgggt catggaaggg catctggatt cgcctaccga
     5461 ggcggaacag aaacacctga ctctggcgca gatttcagca ggctggcgcc tggcgtgccg
     5521 taccatcatc gcgacggaca ctcatgttta catcccggcg aattcattaa cggaaagcca
     5581 ggtcatccaa atcgaaggtg cggaaaccgc cgccgtcccc gatcctgccg ttcgcctctt
     5641 gccgttgctt gctaatcccc cgtctctctc cgaccagata gcggacctgg aacgggtcgc
     5701 caatgccctg cgccagcaac atgctcttga aagtgtctgg gccgggttgc cggcgctgtg
     5761 cgcccttcgt tctgtcttgc gagaaacgga ctggcagatc gaggtcgcat tgcgaggaac
     5821 agagatcatt gccgcctatc cgcttgggca acacagaccg gtggggatgg cagtggatgt
     5881 gggtacgacc aaactggcct gttacctggt tgatctggaa accgggcgca cgctggccgc
     5941 caaaggggtc atgaaccctc aaatcgccta tggagaggat gtcatgtccc gcctggaagc
     6001 cgtcctggtg gagccgggga acgccgaccg gctccaggct gaagtcgtgt cgtcaatcaa
     6061 tgatgtggcg aaagaactct gcgcggcgca gagcctcact ccagaccata tcctggacta
     6121 ctgtttggtt ggaaacacag ccatgcacca cctgctggca aagttgcccg tgcggccgct
     6181 cgccgtctcg ccttttatcc ccacgctcag tgatgtgctg gatatcgagg cggatcgatt
     6241 gggtttgaac gctgcacccg gcgcacgtgc atacctgcct gccccgttgg ccgggtttgt
     6301 cggttccgat cacctggcgt ttctactggc ctccggtttc gatgaaggag accggatccg
     6361 gctcggcatt gatatcggga cgaacaccga gattgctctt caggtcggga gaaggatcat
     6421 aacttgttca actgcttcgg gtcccgcctt cgaaggagcc cacatccacc acggcatgcg
     6481 cgctgctccc ggcgcgatcg aacgcgtaaa gatcacggaa gagggtggga ttcagtgtga
     6541 tgtcatcggt ggagtcccgg cagtaggcat ctgtggctct ggtatcctcg acgccctggc
     6601 tgaaatgtat cgagccagga tcatcaacga acgcgggcgg ttccagctta acgcgcctgg
     6661 cgtggaaatg gccaacgatg attcactgat cttctccctg tcaaaaggtg tgaatggcta
     6721 ccgggatgtc acgctctccc aacacgatgt tgaccaggtg atgctggcca agggcgccat
     6781 ccgggcgggt atcgagatcc tgctcagcaa agcggatgtc gccccagccg atatcgagga
     6841 aatcgtgatt gccggagcct ttgggaccta tttggatccc tgcaatgcgg tacgcatcgg
     6901 actgattcca ggcgtgccag tggaacgaat ccgtgcggta gggaatgcag ccggggcagg
     6961 cgcacgcctt ttgctctctt ccctagaata tcgccgccgg gcgcacgcgc tggcaaaacg
     7021 gatcgagtac gttgaactca ccatcgtgcc cggttttaat aaattttttg cgaaaggtgt
     7081 tcggtttccg acgccagctt aagaatctgg cttctcgcca aaaaaagaat ccagtaacaa
     7141 ggaatcagga aagcaacttt ccacatggag gtaaaagatg tccgaaccgc aatttgacgc
     7201 tatcagcaaa gctgttatcg aaggagagga tgtcgaggcc ggccgcctgg tggaacaggc
     7261 gctcgaggct ggcctggcgc ccctcgatat cctccaaaaa ggagtggtgc acggcatcgc
     7321 ccgggcagga gaattatgga aagccaatga atattttctt ccagat
//
LOCUS       DATMXK010000169         2659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_132110, whole genome shotgun sequence.
ACCESSION   DATMXK010000169 DATMXK010000000
VERSION     DATMXK010000169.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132110"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VN363_03125"
     CDS             complement(<1..455)
                     /locus_tag="VN363_03125"
                     /inference="COORDINATES: protein motif:HMM:NF013412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxamine 5'-phosphate oxidase family
                     protein"
                     /protein_id="HWQ83530.1"
                     /translation="MPDPVPERPYFGTTYGIDPSNEGLLPWSWASEQLGASRNYWIGS
                     TRPDGRPHAAPVWGVWMDGAVYFSSARDSRKARNLAKNPQVVVHLESGDEVVILEGIV
                     AELRDAALYAKIASAYNAKYQGIFMAAEMSAGDLVYGVRPLNAYGWLEQ"
     gene            complement(603..1817)
                     /locus_tag="VN363_03130"
     CDS             complement(603..1817)
                     /locus_tag="VN363_03130"
                     /EC_number="4.3.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560042.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003941 - L-serine ammonia-lyase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0009063 - amino acid catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-serine ammonia-lyase"
                     /protein_id="HWQ83531.1"
                     /translation="MQSIRELYRIGHGPSSSHTMGPRRAAVLVRERYPQAAGWSVTLY
                     GSLAATGRGHMTDRAIQEALDPLPVEFNWRSDEELPLHPNGMLFEAKNETGEVLASLR
                     IYSPGGGAIQIEGEDPPSDAVYNLNSMDEILKYCVQSGKTLWEYVEEREGSSIWDYLA
                     QIYTVMQAAIERGLHAEGVLPGELGLSRRAWTFYRKLQLHGGEFRSEGFMPAYALAVS
                     EENASAHQIVTAPTCGSSGVLPAVLRHVRDTVNTSEIHIFRALATAGLIGNLIKHNAS
                     ISGAEVGCQGEVGAACAMAAAAAAQLMGGSVRQIEYAAEMGLEHHLGLTCDPVSGLVQ
                     IPCIERNVFGATRALDCANFALYSDGSHRVSFDEVVRVMGVTGRDLPSMYRETSEGGL
                     AILYRFPPKRNS"
     gene            complement(1856..2647)
                     /locus_tag="VN363_03135"
     CDS             complement(1856..2647)
                     /locus_tag="VN363_03135"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ83532.1"
                     /translation="MDQTNPFDNHTRLLALYDRHVRIDQEIPDVRKSVTQDVVRFIRP
                     APGMNFISYAWLDETTADKVILNQIEYFLKFDQPFTWQMMEHDLPADLGERLIKHGFV
                     GDDDPSAVMLLDLEHCPPELLQPTRLDVRRITTPEGLDDVVKIEEQVLGGNFAWLKQR
                     LAAHLQISDYLSVYVAYADGKPASTAWTYFLPHNPFAGLFGGATLPEQRKKGLYTALV
                     ARRVQEAVQRERRFLSVGCSPNSRLIVARHGFEFIGYQYDFEYQR"
BASE COUNT          605 a          791 c          691 g          572 t
ORIGIN      
        1 tcctgctcca gccagccata ggcattcaga ggccggactc cgtaaaccag gtctcccgca
       61 gacatttccg cagccataaa aataccctga tatttcgcgt tgtacgcact ggcaatcttt
      121 gcatatagcg ctgcgtcccg aagctctgcc acgattcctt ccaggattac cacttcatcc
      181 ccgctttcca gatgcaccac aacctgtgga ttcttggcca ggttgcgggc tttgcgcgag
      241 tcgcgcgctg agctaaaata taccgcccca tccatccata cgccccagac cggggcagca
      301 tgcggtctgc catcaggacg ggtagatccg atccagtaat tgcgggaggc acccagctgc
      361 tcgctggccc aactccaggg tagcaatcct tcgttggatg gatcaattcc ataagtagtc
      421 ccaaaatatg gtcgctctgg aacaggatct ggcatcctca ttacactacc tcctcatcaa
      481 atctaaaaat accactcgct cgatggaatc atagctggtg cgcaggcatc ttgaatacaa
      541 accattccgt ccgcaggttc accgggttca aggatcaggg tttgaattta ttacggaatt
      601 gctcaggaat ttcgtttcgg cggaaagcgg taaaggatcg ccagcccacc ctcagaagtt
      661 tcgcgataca tggaaggcag gtcacgcccg gtgaccccca tcacgcgcac gacttcatca
      721 aacgagacgc gatggctgcc atccgagtac agggcaaagt tagcgcagtc cagggcacgc
      781 gtggcgccaa atacattgcg ctcaatgcag ggtatctgca ccaacccact gacggggtca
      841 caggtcagac ccaaatggtg ttccaggccc atctccgccg cgtactcgat ctggcggact
      901 gagccgccca tcaactgagc agctgcagcc gcagccatcg cacaggctgc gcccacttcc
      961 ccctggcagc ctacttcggc acctgagatc gaggcgttgt gtttgatcag gttgccaatt
     1021 aacccggcgg tcgccagagc ccggaatata tggatttcgc tggtgttgac tgtatcgcgc
     1081 acatggcgta ataccgccgg cagcaccccg ctcgagccac acgttggggc tgtcacgatc
     1141 tgatgggcag aagcattttc ctctgacacc gccagcgcgt aagcaggcat aaatccctcc
     1201 gagcggaatt cgcctccgtg cagctggagc ttgcggtaaa aggtccaggc gcgccgtgag
     1261 aggcccagct cgccgggtaa gaccccctct gcgtgcagac cgcgctcaat agcagcctgc
     1321 atcacggtgt agatctgcgc cagataatcc cagatactgc tgccttcgcg ctcctcgaca
     1381 tattcccaca aagttttacc agactggacg cagtacttga ggatttcatc cattgagttg
     1441 aggttataca cggcatccga tggtggatct tccccctcga tctggatcgc tccaccgccc
     1501 gggctgtata tcctgagcga tgctaaaacc tcaccggtct catttttagc ttcaaacagc
     1561 attccattgg gatggagggg cagctcttcg tcagaacgcc agttaaattc gaccggcaag
     1621 ggatccaggg cttcctggat ggcccggtcg gtcatatgcc cacgcccggt tgctgccagg
     1681 ctgccgtaca gggtaacgct ccagcctgct gcctgcgggt aacgctcccg gacgagcaca
     1741 gccgcccggc gtggacccat cgtgtggctg ctggagggac catgccctat gcgatataac
     1801 tcccgaatcg attgcatacc tttaccttat aaaagccgtt ccggcgggac agcgatcagc
     1861 gctgatattc aaaatcatac tgatacccga tgaattcaaa cccatgccgg gcaacgatca
     1921 gacggctgtt tgggctgcag cccacgctca agaagcgccg ttcacgctgc acagcttcct
     1981 ggacacgccg ggctaccagg gcggtgtata aacccttttt gcgctgctca ggcagagtcg
     2041 ccccgccgaa caagccggca aatggattat gaggcaggaa gtaggtccag gctgtgctgg
     2101 ctggcttacc gtcggcataa gcgacataga cgctcaggta atctgaaatt tgcagatggg
     2161 ctgctaaacg ctgcttgagc caggcaaaat tcccccccag gacctgctct tcgatcttta
     2221 cgacatcatc caacccttca ggtgtggtga tccggcgcac gtccaggcgg gtaggttgga
     2281 gtaattccgg cgggcagtgc tccagatcca ggagcatgac cgcgctggga tcatcatcgc
     2341 caacaaaacc gtgcttaata agacgttcgc ccagatcagc aggtaggtcg tgctccatca
     2401 tctgccaggt aaaaggctgg tcaaacttga gaaagtattc aatttgattc aggatcactt
     2461 tatccgcggt cgtttcatcc agccaggcat aagagataaa attcatcccc ggtgcgggtc
     2521 gaataaaacg aaccacatcc tgggtgacgc tttttcttac gtctgggatt tcttgatcaa
     2581 tgcgaacatg ccggtcatac aatgcgagaa gtcgagtgtg gttatcaaaa ggattcgtct
     2641 gatccatagc ttacctgac
//
LOCUS       DATMXK010000170         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_92804, whole genome shotgun sequence.
ACCESSION   DATMXK010000170 DATMXK010000000
VERSION     DATMXK010000170.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92804"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..514
                     /locus_tag="VN363_03140"
     CDS             <1..514
                     /locus_tag="VN363_03140"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83533.1"
                     /translation="VPVDAAYQIYGLIYDIHIDDDGLVRQLVTADGVDEHIIADNRIN
                     RNVPLEMSVLSVGYRQNGRIFHMLPPRPALGLDMIYLCDASEICEFTRSGRFGYFRHI
                     LRAQDLPVGELLAAHVQQAHQAHCLVGEPSWTVAATRELITLLRDDYPTLMSVLGALG
                     DAQLGDNGRA"
     gene            522..2252
                     /locus_tag="VN363_03145"
     CDS             522..2252
                     /locus_tag="VN363_03145"
                     /inference="COORDINATES: protein motif:HMM:NF014044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83534.1"
                     /translation="MNDPMAAGGNEATIGYIVGGGLRENLHVRLTVPAQQVQEGSFVV
                     VESGEWLFHGLVTDLQLGATDPRFADEQSEARFPAELANLLHGQTLYTNLELLPALML
                     ERGPEIDSPEYSRWRDAIDAGLREEPRPIPVKTVPPHHAPVRMAGSGDIAEIFGKVDS
                     KGNFFIGTTREQGHPVCINLDKFIQRSSGVFGATGTGKSFLTRIVLAGLIHYDRASVL
                     VLDMHNEYGYDDTASDTGMRVIGLKSKFASKVRVVGLGKGALVRGEAPDFNLEIAELD
                     IQPEDIELLTRELNLKETTPTTLDALVSNFGRQKWFSAFKQMRNGAVIEDDAGKKIPA
                     ADSVAAWANSAGINVMAAEGLYSKLGRVFGLPYVVEKPAVDTVSEIIKALEAGQHIVL
                     SFGDNVSDLDYLLVSNLLTRRIRQAWETRTNEFRSAKSAEPRPLVIVVEEAHKLLNRE
                     MASQTTFSTIARELRKYYVTLLVIDQRPSQIYDEVMSQLGTRISGWLGDEDDIRAVLS
                     GLAGRDALRGMLAHLQPKEEVLLLGWGVPMPLPVRSRRYDDVFWKELLGHAAPISRRS
                     PDEIAKELGF"
     gene            2276..3094
                     /locus_tag="VN363_03150"
     CDS             2276..3094
                     /locus_tag="VN363_03150"
                     /inference="COORDINATES: protein motif:HMM:NF012472.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWQ83535.1"
                     /translation="MRYRELPAGDRIPVIGLGTWRMGGGSEPDATQNELVLHAIRTAL
                     KLGYTHIDTAEMYGGGHTEELIGEVIRDFDRESLFITSKVWHTNLQYPDVLRACENSL
                     GRLGTDYLDLYMIHWPNSGVPLEETFRALNKLVEDGNVRHLGVSNFDLALLTAARQLS
                     IVPLVTDQVPYSVQNRTYAKNGVLAYCQEQDMLLTAYTPLDKGRVDDLEKLNVIARRA
                     GATPMQVALSWLVSQPNIIAIPKSFNPDHLRQNLEAVDLVLDEDELNILDHLAR"
BASE COUNT          747 a          782 c          969 g          761 t
ORIGIN      
        1 agttccagtt gatgctgctt atcaaattta tggattaatc tacgatatcc atattgacga
       61 cgatgggttg gttcgccagc tcgtgactgc cgatggggtc gatgagcaca tcatcgcgga
      121 taatcgtatc aatcgcaacg tcccattgga gatgagtgta ttgagcgtcg gttaccgcca
      181 gaatgggcgg atttttcata tgctcccgcc gcgtccggcg ctgggtctgg atatgattta
      241 tctgtgtgac gcgagcgaaa tctgtgagtt tacccggtca ggccgctttg gctatttccg
      301 gcatatttta cgagcccagg acctgccggt tggtgaattg ctggcggcac acgtacagca
      361 ggctcaccag gcgcactgcc tggtgggtga gccgtcctgg accgtggcgg caacccggga
      421 attgataact ttgctgcgcg atgattaccc cacactgatg agtgtgctgg gagctctggg
      481 tgatgctcaa ttgggcgata atggaagggc gtaacatgga aatgaacgat ccaatggctg
      541 ccggtgggaa tgaagccaca atcggttata tcgtcggtgg cgggttgcga gaaaacctgc
      601 atgttcgcct gacggtacct gcccagcaag tccaggaagg tagtttcgtg gttgttgaga
      661 gcggagaatg gctgtttcat ggtttggtga ccgatctgca gctgggggca acggacccac
      721 gttttgcaga cgaacaaagc gaggcgcgtt ttccggccga gctggcaaat ttgctgcatg
      781 ggcagaccct ttacaccaat ctggagctgc tgccggcact gatgctggaa cgcggccctg
      841 aaatcgacag tccggaatac tcgcgctggc gagatgcgat tgatgctggt ctgcgcgaag
      901 aaccccgtcc gatccctgtc aagactgtcc ctcctcacca cgccccggta cgcatggcag
      961 gatcgggaga tatcgccgag atattcggaa aagttgacag caaaggcaat tttttcattg
     1021 gcaccactcg cgagcaaggt catccggtgt gcattaatct ggacaaattt atccagcgct
     1081 cgtcgggcgt attcggcgca accggtacag gcaaatcctt tctgacccgc attgtactgg
     1141 cgggcttgat tcattacgac cgcgcgtctg tcctggtgct ggatatgcac aatgagtacg
     1201 gttacgacga caccgcttcg gataccggaa tgcgggtgat cggcttgaag agtaaatttg
     1261 ccagcaaagt acgtgtggtc gggctgggaa aaggcgccct ggtgcgagga gaagccccag
     1321 acttcaacct tgagatcgcc gaactggata tccagcccga ggacattgag ctcctgacta
     1381 gggagctgaa cctgaaggaa acgacgccca ccaccctgga tgcactggtg agcaattttg
     1441 ggcgtcaaaa atggtttagc gcctttaaac aaatgcgcaa cggggctgtg atcgaggatg
     1501 atgccggcaa aaagatcccg gctgccgaca gcgtagctgc ctgggccaac tcggctggca
     1561 tcaatgtaat ggcggctgaa ggtttatatt caaagctggg acgcgtcttt ggcttgcctt
     1621 atgtggtgga aaagccggcg gttgatacgg tcagtgagat tatcaaggcg ctggaagctg
     1681 gtcagcacat tgttctatca ttcggggata atgtcagcga tctggattat ttactggtga
     1741 gcaatctatt gacccgccgt attcgccagg cgtgggaaac gcgcaccaac gaatttcgca
     1801 gtgctaaaag cgctgaacca cgcccgttgg tgatcgtggt ggaagaggcc cacaagctgc
     1861 tcaatcgtga aatggcttcc cagacgactt tcagcacgat tgcgcgtgag cttcgcaaat
     1921 attacgttac gctgctggtg attgaccagc gaccatccca gatctatgat gaagttatgt
     1981 cgcagttggg gacgcgcatc tcaggctggc tcggagatga agatgatatc cgggcagtgc
     2041 tctccggtct ggcagggcgg gatgctttgc gagggatgtt ggcccatctc caaccgaaag
     2101 aggaagttct gctgctgggt tggggtgtgc cgatgccttt gccagtgcgc tcgcgccggt
     2161 atgatgatgt tttctggaaa gagttactcg gacatgccgc tcctatatcc cggcgtagtc
     2221 ctgatgaaat cgctaaagaa ctgggattct gaggtaaggt atagaaatgt gtgccatgcg
     2281 ctatcgtgaa ctgccggctg gtgaccggat ccctgtgatc ggtctgggca cctggcggat
     2341 gggaggcgga tcggagcctg atgcaactca gaatgagctt gttttgcatg caatccggac
     2401 tgccctgaag ctgggatata cccacatcga tactgcagaa atgtatggcg gtggtcacac
     2461 agaagagctg attggtgaag tgattcgcga ttttgaccgg gaatccttat ttattacgtc
     2521 caaggtgtgg cacacgaatt tgcaataccc ggatgtcttg cgcgcctgtg aaaacagcct
     2581 gggccggctg gggacggatt atctggatct ttacatgatt cactggccaa attctggagt
     2641 ccccctggag gagacattcc gagcccttaa caaacttgtg gaagatggca acgtacgtca
     2701 cctgggggtg agtaatttcg atctcgccct gctcactgct gccaggcagc tttcgattgt
     2761 tcccctggtg actgaccagg tcccctacag cgtccagaac cgtacctatg ctaaaaatgg
     2821 ggtgctggca tactgccagg agcaggatat gctgttaacc gcgtatactc cgctggataa
     2881 aggtcgcgtg gatgatctgg aaaaactgaa tgtgattgcc agacgtgctg gagcaacccc
     2941 tatgcaggtg gctcttagct ggctggtcag ccagcccaat atcattgcta tcccaaaatc
     3001 attcaaccct gatcatttac gccagaacct ggaagctgtg gatctggttt tggatgaaga
     3061 tgagctgaat attctcgatc atttggctcg ctgaacggaa gatggagtaa ccaggtgaaa
     3121 attgcgacat tgtgctatat ccggtccggg gaaaggacat tgatgatcta ccgcaacaaa
     3181 aagcccgacg acatgcatgc aggtaaatgg aatgggttgg gcggaaaatt ggaagccggg
     3241 gaaacaccag aagagtgcg
//
LOCUS       DATMXK010000171         2959 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_71797, whole genome shotgun sequence.
ACCESSION   DATMXK010000171 DATMXK010000000
VERSION     DATMXK010000171.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2959)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2959)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2959
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71797"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..625)
                     /locus_tag="VN363_03155"
     CDS             complement(<1..625)
                     /locus_tag="VN363_03155"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561154.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldo/keto reductase"
                     /protein_id="HWQ83536.1"
                     /translation="MEYINLGRSGLKISRIGLGMMSYGTPSWRPWVLDEASSRPFIQK
                     ALESGINFFDTANGYSLGMSEEVTGRALRDFSRREEVVIDTKVFFPTGDSPNQRGTSR
                     VHIMNAVDASLRRLGTEYIDLYQIHRWDPETPVEETLEALHDVVKAGKARYIGASSMY
                     AWQFTQALYLADIHGWTRFISMQNHYNLIYREEEREMLPFCRAQGIGI"
     gene            complement(653..1243)
                     /locus_tag="VN363_03160"
     CDS             complement(653..1243)
                     /locus_tag="VN363_03160"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="O-methyltransferase"
                     /protein_id="HWQ83537.1"
                     /translation="MFHPIPSPVRQRMEFLEAQDERDRSDGTPHALRLRQIPPETGRF
                     LALLASAAPQGAILEIGTSAGYSSLWLSLACRHTQRRLTTFELSQAKASLAAETFRQA
                     EVEPWVELVNADALARLVDYPQIAFCFLDTEKSLYQSCYDLIVPRLVPGGWLVADNVI
                     SHAAELQPMLNHALQDERLDAAIFPIGKGLLVCRKP"
     gene            complement(1248..>2959)
                     /locus_tag="VN363_03165"
     CDS             complement(1248..>2959)
                     /locus_tag="VN363_03165"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR02289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="M3 family oligoendopeptidase"
                     /protein_id="HWQ83538.1"
                     /translation="NMPAKLPSTVAEIKDWPWSQLEPFYAELGSRALTSATLAAWMAD
                     WSDLQRLVSEWFSRLSVAITVDTTDQEAERRYKQYLDEIYPRANEWNQKLKEKLLASG
                     LEPTGFAVPLRNMRTEAAIFRQENLALESEELKLGTEFDKTIGAQTVQWEGQELTISQ
                     LRPIYLDPDRSRREKAWRLGSQRQLADRQTLNGLFNKFLALRRQMAANAGFSDYRAYR
                     WKQYLRFDYTPENCRQFHTAIEQVVVPAAEKIYARRKAQLGVDSLRPWDLEVDPLDRP
                     ALRPFSEIPVLEGTVESIFHNVDDQLGKYFGIMRREKLLDLENRKGKAPGGYCTDFSA
                     VKRPFIFMNAVGLHDDVQTLLHEGGHSFHVFETASLPYYQQLQVPMEFAEVASMGMEL
                     LAAPYLADEQGGFYTPADAARARIEHLEGCILFWPYMAVVDAFQHWVYENPDAAVDSA
                     RCDQTWGGLWDRFMRGVDWSGLEEEKITGWHRKLHIFQVPFYYVEYGLAQLGAMQIWR
                     NALQDQAKAVANYRKALALGGTVTLPELYTAAGAKFTFDPQTLGEVVDLAMETIDELE
                     KVG"
BASE COUNT          630 a          821 c          909 g          599 t
ORIGIN      
        1 gtatcccgat cccctgcgcc cggcaaaagg gcagcatttc gcgttcttct tcacgataga
       61 tcagattgta gtggttctgc atcgagataa agcgcgtcca gccgtggata tccgccaggt
      121 agagagcctg ggtgaactgc caggcataca tgctggaggc gccaatatag cgggccttgc
      181 cggcttttac cacatcatgc aaggcttcca gggtttcttc aacgggcgtt tcgggatccc
      241 agcggtggat ctggtacagg tcgatatact ctgtgccgag gcgccgcagg gaagcatcga
      301 cggcgttcat gatatgaacg cgggaagtgc cgcgctggtt agggctatcg ccggtgggaa
      361 agaagacttt tgtgtcgata accacttctt cacggcggga aaaatcgcgc aaggcgcgcc
      421 cggttacctc ttcgctcata cccagggaat acccgttggc agtgtcaaag aagttgatgc
      481 cggattcgag tgccttctgg atgaaggggc ggctggaggc ttcatctaaa acccatggcc
      541 gccagctggg cgtaccgtag ctcatcatcc cgagaccaat gcgggagatc tttaacccgc
      601 tgcgacccag attaatatat tccatcgtga tctaaaccac ctttctcaac ccttacggct
      661 tgcgacagac aagcaaacct ttcccaatgg ggaagatcgc ggcatccagg cgttcatcct
      721 gtaatgcatg attgagcatg ggctgtaatt ctgccgcatg gctgatgaca ttgtcggcga
      781 ccaaccagcc tcccggcacc aggcggggaa caatcaggtc gtagcaggac tggtagagag
      841 atttctcggt atcgagaaaa caaaatgcga tctggggata atccaccagc cgcgccaggg
      901 cgtccgcatt gaccagctca acccagggct caacctcagc ctggcgaaag gtttcggcag
      961 ccaggctggc tttggcctga gagagctcaa aagtggtcag acggcgttgg gtgtggcggc
     1021 aggccagaga gagccataag ctggaatacc cggcgctggt gccgatctcc aggatggcgc
     1081 cctgaggagc ggcgctggcg agcaaagcca ggaagcggcc tgtctcaggc ggaatctggc
     1141 gcaggcgcag ggcatgcggc gtaccatcac tgcgatcgcg ctcgtcctgc gcctccagaa
     1201 actccatccg ctggcggacc ggcgaaggga tggggtggaa catggcgcta acctaccttt
     1261 tccagctcgt caatggtctc catggccagg tccaccactt caccgagagt ctgcggatcg
     1321 aaggtgaatt ttgccccggc agcggtatag agctcgggca gggtaaccgt gccgcccagg
     1381 gcaagggctt tgcggtaatt ggcgactgcc ttcgcctggt cctggagggc attgcgccag
     1441 atttgcatgg cgcccagctg ggcaagacca tattcgacgt aatagaatgg gacctggaaa
     1501 atgtgcagct tgcggtgcca gccggtgatc ttttcttctt ccaggccgct ccaatccaca
     1561 ccgcgcatga agcggtccca caatccgccc caggtctggt cgcaccgggc cgagtccacg
     1621 gcggcatccg ggttctcgta cacccaatgc tggaaggcgt cgaccaccgc catatacggc
     1681 cagaacagga tacaaccttc caggtgctcg atccgggcgc gggcggcatc ggcgggggta
     1741 tagaagccac cctgctcatc agccaggtaa ggcgcagcga gcagctccat ccccatggaa
     1801 gccacctcgg caaattccat gggaacctga agctgctggt agtaaggcag gctggcagtc
     1861 tcgaagacat ggaaagaatg cccgccctca tgcaagaggg tctggacatc gtcgtggaga
     1921 ccaaccgcgt tcatgaaaat gaagggccgt tttacagccg agaaatccgt gcagtagcca
     1981 ccgggagctt tgcctttgcg gttttccaga tcgagcagtt tttcgcggcg catgataccg
     2041 aaatattttc cgagctgatc atcgacatta tgaaagatgc tttccacggt gccttccagg
     2101 accgggattt cagagaaggg acgcagggcg gggcgatcca ggggatccac ctctaaatcc
     2161 caggggcgca gggagtctac accgagctgt gccttgcgcc gggcatagat cttttcagcg
     2221 gcgggcacga cgacctgctc aatggcagtg tggaactggc ggcaattctc aggtgtgtaa
     2281 tcgaagcgca ggtactgctt ccagcggtag gcacgataat cagagaaacc ggcgttggca
     2341 gccatctggc ggcgaagggc caggaatttg ttaaacagcc cattgagggt ctgccgatcg
     2401 gcaagctggc gttggctgcc caggcgccag gctttttcgc ggcgggagcg atcgggatcc
     2461 aggtagatcg ggcgcagctg agaaatggtg agctcctggc cttcccattg gactgtctgc
     2521 gcgccgatgg ttttgtcgaa ttccgtgccc agctttagct cttcactttc aagggccagg
     2581 ttttcctggc ggaaaatggc cgcctcggtg cgcatattgc ggagtggaac cgcaaacccg
     2641 gtgggctcca ggccgctggc gagaagcttc tctttaagct tctggttcca ttcattggcc
     2701 cgcgggtaga tttcatccag gtattgtttg tatcgccgct cggcttcctg gtcggtagtg
     2761 tcgacggtaa tggcgaccga caggcgggaa aaccattcac tgaccaggcg ctgaaggtcg
     2821 gaccaatccg ccatccaggc tgcgagagtc gccgaagtga gggcgcgaga gcctaattca
     2881 gcgtaaaagg gttccagctg gctccagggc cagtctttga tttcagcgac agtggaaggt
     2941 aacttggcag gcatattca
//
LOCUS       DATMXK010000172         3251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_33628, whole genome shotgun sequence.
ACCESSION   DATMXK010000172 DATMXK010000000
VERSION     DATMXK010000172.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_33628"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VN363_03170"
     CDS             complement(<1..234)
                     /locus_tag="VN363_03170"
                     /inference="COORDINATES: protein motif:HMM:NF014068.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DJ-1/PfpI family protein"
                     /protein_id="HWQ83539.1"
                     /translation="MKLANKKIAILLEEGFEDLEFWVTVMRLREEGAQVTVLGLKAGK
                     TVHGKVALPATSDKAYSEVKPDDFDGLVVPGGCC"
     gene            complement(313..690)
                     /locus_tag="VN363_03175"
     CDS             complement(313..690)
                     /locus_tag="VN363_03175"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83540.1"
                     /translation="MINNPSTPKPGESTTLWFIKLVTGPILLVVAFIHLVINHYVGSV
                     NGLLTHAEVVAYYQSWYIPVMEAIFLITVISHALIGLRGIILDLKPAPGLLRILDWIF
                     ILGGAGFVVYGMWLLQAVTQFGR"
     gene            complement(700..1086)
                     /locus_tag="VN363_03180"
     CDS             complement(700..1086)
                     /locus_tag="VN363_03180"
                     /inference="COORDINATES: protein motif:HMM:NF013307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83541.1"
                     /translation="MNTSPNIFARIFRWFDPRGRTLGDYGFILNRLTALGLTLYLFLH
                     LIVLGNLARGPEAYWEFLVVIESPLYKIGEWLVIAAVLFHGLNGIRIGLNSFGIAVPQ
                     QRQYLIIVMLLSLIGVAFFGWKMFLG"
     gene            complement(1083..1838)
                     /locus_tag="VN363_03185"
     CDS             complement(1083..1838)
                     /locus_tag="VN363_03185"
                     /inference="COORDINATES: protein motif:HMM:TIGR00384.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2Fe-2S iron-sulfur cluster-binding protein"
                     /protein_id="HWQ83542.1"
                     /translation="MAEKWNITLVIRRQKQGQEPYDQAFAMEANPDENVIDLVERVWA
                     FHDSTLNYAHACHHSTCGACGMRVNNIEKLTCITPVRSVVSDGGTLRVDPMRNFPVIS
                     DLAVDMGELYRRMELVGHQPVNSDRKEAIQKEFRPSAPTRPGEDFIRLSDCIECGLCM
                     SACPIVGSTPDYLGPAPLAGAHQAGLKGNSHLVDLVGGTDGVWRCHSAYECTAVCPSN
                     VDPGWRIMELRRDLLGEKIKSWFGSSKTAEVRK"
     gene            complement(1850..3019)
                     /locus_tag="VN363_03190"
     CDS             complement(1850..3019)
                     /locus_tag="VN363_03190"
                     /inference="COORDINATES: protein motif:HMM:NF013434.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ83543.1"
                     /translation="MAKPNILIIGAGFTGVATAHDLALRGCDVTVVERGPIANGTSGR
                     THGLLHSGGRYAVKDQESAIECIDENMILRKIVPSVIEPNGGLFVALNEKDVAYGEDF
                     IAGCEACHIPIEEVSAQQALQMEPYLNPGVLAAFTIPDGTFDPLRLALAFAATAKKHG
                     AKFLLYHEVKGLLLDGQGNVTGVKVIDLPANKEKELRADIVVNATGAWVGEITAMAGA
                     DVPIHPTPGIMVAFDQRTCQRVINRLCPPADGDIILPQRRMMVVGTTSFTAENLDYIP
                     IIEEQINEMLARAYELSPQLKNAKLRGIYNATRPLVGGGSTGRSIARTFKCFDHKESD
                     NVDGFVTITGGKATTLRAMAEKTTDVVLGKLGLNIPCQTMDYPLVSYREFYALNN"
     gene            complement(3049..>3251)
                     /locus_tag="VN363_03195"
     CDS             complement(3049..>3251)
                     /locus_tag="VN363_03195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83544.1"
                     /translation="ARWRLGVELVDLISFRPPFERIPFGWYACDCGHTGFKPGPVEEF
                     TEESLDLDHQVSDCPACCQKYR"
BASE COUNT          744 a          927 c          872 g          708 t
ORIGIN      
        1 acagcaccca cccggcacga ccagaccatc gaaatcatcc ggtttcacct cgctataggc
       61 cttatcactg gtagccggca aagctacctt gccatgcacg gttttgccgg ctttcaggcc
      121 cagcacggtc acctgtgcgc cttcttcgcg caagcgcatg acggtgaccc aaaattccag
      181 gtcttcaaaa ccctcttcca agaggatggc gatcttttta ttggccagtt tcatgcttcc
      241 tccaatccta gaaataatca ggcgagttag ccgacaggaa catactctcc cgtccgggat
      301 ataccggccc ctttacctac cgaattgggt gacagcctgc agcagccaca tcccgtagac
      361 gacaaatccg gcgcctccga ggataaagat ccaatccagg atccgcagta agcccggggc
      421 tggctttaag tcgaggatga tgccgcgcag cccgatcaga gcgtgagaaa tgaccgtgat
      481 caggaaaatt gcttccatga ccgggatata ccagctctgg taataggcga ctacttcggc
      541 atgggtcagt aaaccattga cgctgccgac ataatgattg atcaccagat ggataaaggc
      601 tacaaccagc aggattggtc cggtaactaa cttgataaac cacagcgtgg tcgattcgcc
      661 cggtttggga gtagatgggt tattgatcat ggctgcctcc tatccgagaa acatcttcca
      721 accaaaaaag gcgacgccga tcaggctgag cagcatcaca atgatgagat actggcgctg
      781 ctgcggtacg gcgatgccaa agctgttcag cccaatgcga atgccgttca agccgtggaa
      841 aagcaccgcg gcgatgacca gccattcccc gattttatag agggggcttt cgatcacaac
      901 caggaattcc cagtaggctt caggacccct ggccaggttg cccagcacaa tcaaatgcaa
      961 aaacaggtac agagtcaggc cgagggcggt gaggcgattc aggataaagc cataatcacc
     1021 gagcgttcgt ccgcgaggat cgaaccagcg gaaaattcgg gcgaagatat tcgggcttgt
     1081 attcatttgc gcacctccgc ggttttactc gagccaaacc agctttttat tttttctccg
     1141 agcagatcgc gacgcagctc catgatgcgc cagccaggat cgacattgga tgggcagaca
     1201 gcagtacact cgtaggcgct gtggcagcgc cacacgccgt cggtgccacc taccaggtcg
     1261 accaggtgag aattgccttt caaacctgcc tggtgtgccc cggccaatgg cgccgggcca
     1321 aggtaatcgg gtgtggagcc tacaatcggg caggcgctca tacacagccc acactcgatg
     1381 cagtcggaca agcggatgaa atcctcaccg ggtcgggtag gtgcggaggg gcggaattct
     1441 ttctggatcg cttctttgcg gtcagaattg actggttggt gacccaccag ctccatgcgc
     1501 cggtaaagct cgcccatatc gacagccaga tcgctgatga ccgggaaatt gcgcatggga
     1561 tcgacccgca gcgtgccgcc atccgatacg actgagcgga ccggggtgat acaggtaagc
     1621 ttttcgatgt tattaacccg catgccgcac gccccacagg tcgagtggtg acaggcgtgg
     1681 gcataattca gagtgctgtc atggaaggcc cagacccgct caacgagatc aatcacgttt
     1741 tcgtccgggt tggcttccat ggcgaaagcc tggtcatacg gctcctggcc ctgtttctgg
     1801 cggcgaatca ccagggtaat gttccatttt tcagccattt ttgtgctcct tagttgttca
     1861 gggcgtagaa ctcccggtag gagaccaacg ggtagtccat cgtctggcag gggatattaa
     1921 gaccgagctt gccgagcaca acatcggtgg tcttttcagc catcgcacgg agcgtggtcg
     1981 ctttaccgcc ggtgatcgtg acaaagccat ccacattatc cgattctttg tggtcaaagc
     2041 atttaaacgt ccgggcgata ctccgcccgg tgctgccacc acctaccagt gggcgggtgg
     2101 cattatagat cccgcgcagt ttagcatttt tcagctgcgg tgacagctcg tatgcccggg
     2161 ccagcatctc gttgatctgt tcttcaatga tcgggatgta gtccagattc tcagcagtga
     2221 aggaagttgt ccctactacc atcatccgcc gctgcggaag aataatatcg ccatctgcag
     2281 gtggacacag gcggttgata acccgctggc aggtgcgctg atcaaaagca accatgatcc
     2341 cgggggtggg atgaataggc acatctgcgc cggccatggc tgtgatttca cccacccatg
     2401 ctccggtagc attcacgaca atatcggcgc gcagttcttt ttctttgttc gccggcaggt
     2461 cgatgacttt gaccccggtt acattcccct gtccatccag gagcaagccc ttgacttcgt
     2521 gatacagcag gaattttgca ccgtgttttt ttgctgtcgc tgcaaaagcc agcgccaggc
     2581 gtaagggatc gaatgtgcca tccgggatcg taaaagcggc cagaacgcca ggattcaggt
     2641 atggctccat ctgcagcgcc tgctgagcgg atacttcttc gatcgggatg tggcaggcct
     2701 cgcagccagc gataaaatcc tcgccatacg cgacgtcctt ttcattcaga gccacgaaca
     2761 agcccccatt cggctcgatg accgatggca caatcttgcg taagatcatg ttttcatcga
     2821 tacattcgat cgccgactcc tggtctttaa ccgcataacg cccgccgctg tgcagcaggc
     2881 catgcgtgcg accggatgtg ccgttggcga tcggaccacg ctcaacgacg gtaacatcac
     2941 agccgcgcag ggccaggtca tgggcggttg ccacgcccgt aaaccctgcc ccaatgataa
     3001 gaatgtttgg ttttgccata ggttttgatc ctttcgtgaa ggattaattt aacggtactt
     3061 ctgacaacaa gccgggcagt cgctcacctg gtggtccaga tccaggcttt cttcggtgaa
     3121 ttcctctacc ggccctggtt tgaacccggt gtgaccacaa tcacaagcat accacccaaa
     3181 cgggattcgc tcaaagggcg ggcggaaaga gatcaaatcg accagctcaa ctcccagcct
     3241 ccagcgagca t
//
LOCUS       DATMXK010000173         4688 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_129897, whole genome shotgun sequence.
ACCESSION   DATMXK010000173 DATMXK010000000
VERSION     DATMXK010000173.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4688)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4688)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4688
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129897"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(50..964)
                     /locus_tag="VN363_03200"
     CDS             complement(50..964)
                     /locus_tag="VN363_03200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560908.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S cluster-binding domain-containing
                     protein"
                     /protein_id="HWQ83545.1"
                     /translation="MEPLEPAYLSLLHSGELEKRAASAHAGLAECRQCAWACRANRLA
                     GKTGACKTGLLAEVSSYGAHPGEERPISGSGGSGTVFFARCNLRCQYCQNAEISQLQP
                     GEPMSPEALAGIFLELQAQGCHNINLVSPSHVVTQILAAVLIAARQGLNLPLVYNTGG
                     YDSLDALHLLDGVIDIYMPDMKYSSAAIAKYYSKIPNYPQVNQAAVREMYRQVGDLML
                     DEHGLARRGLLIRHLVLPGDLAGSAEILHFIAADISPDTYLNLMDQYHPAFNARLYPR
                     LRRPVSQAEYRSVVELAQKTGLKRFHTD"
     gene            complement(1114..1314)
                     /locus_tag="VN363_03205"
     CDS             complement(1114..1314)
                     /locus_tag="VN363_03205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83546.1"
                     /translation="MSLSPLKLMVIGTLLMILGVVLPLMMVVKLLTASFALSFISYAA
                     SLIGMFMAFLGLFTYVKVNRRK"
     gene            complement(1304..2347)
                     /locus_tag="VN363_03210"
     CDS             complement(1304..2347)
                     /locus_tag="VN363_03210"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83547.1"
                     /translation="MSDTLQVTDKIQTIPAPGADFPLLSVQDLHVWFELRRFGFGHAG
                     YVRAVDGVSFNLDHGEAVAVVGESGCGKSSLMKTILGLNKITKGDIVYNGKKLSEYSS
                     QDMRWYRSQVGYVQQDPYGALPPFMNIQQILEEPLIINGVKDKTERLERITKALEEVK
                     MTPVDEFLPKFPHMISGGQQQRVVIARAMILNPKLLVADEPVSMLDASVRVEILRLLR
                     GLQENHDLAVIYITHDLSTVRYFSEYIFVMYAGEIIEKAQVDDLIRNPKHPYTLALLA
                     ATSDPDAKNVDEFQEVPPGEPPSLVKPPSGCRFHPRCKFMMPGLCDVERPPEFNIEPE
                     HLAACWLFKENES"
     gene            complement(2366..3322)
                     /locus_tag="VN363_03215"
     CDS             complement(2366..3322)
                     /locus_tag="VN363_03215"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83548.1"
                     /translation="MAEKALLQVKNLKLYFKAARGLVQAVDGVDLTLAPNEAIVIIGE
                     SGCGKSSMAKALLRLLPRNVGTYSGEVFLDGVDIMKLDEEKFRRDVRWVKMAMVPQAA
                     MNSLNPVLRVGDQVAEPMMVHHGVSKEAALDHAKTMFQRVGVPVDFVDRYAFELSGGM
                     RQRVAIAMSLVAVPDLVILDEPTSALDVLTQANIMNLLKRVKKEIATSFILITHDIST
                     SSELADRVAVMYAGQIVEVSDNHRFFTRPLHPYSAALMSSVPRLRSTANLDFISGQPP
                     SLINPPTGCRFAARCPRVFEKCVQDPPTISQDGRLVKCWLYT"
     gene            complement(3328..>4688)
                     /locus_tag="VN363_03220"
     CDS             complement(3328..>4688)
                     /locus_tag="VN363_03220"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83549.1"
                     /translation="WGELAMNSLKKTLRDIAQYPSAIFGMIIVLALVSLAVYAVVTIP
                     YNEAIRLWRGGEEIWYQNPKNVPPAWFNYFTARDLPESFAVKSGEEGFTKEVIPGEQN
                     TTTINFSYPIEFNYDDYPQEILLYFNTTYEAKQPFVSIYLNTPDGREIRISDFGVSRK
                     QTYRFSQDEKLVRRLKGVVTMKGLFSVPESDPLTPLKGTYELVINGLTFEQGSDINAE
                     LVFHGQLFGIAGTDHARRDLSVALLWGAPIALAFGLLAAAGTSVLTMIIAAIGTWYSG
                     WVDELIQRITEVNLVLPFLPILIMIGTFVDRSIWTILGATIALSIFTGSIKTYRAIFM
                     QVKESTYIEAAKAYGASDGRVIFKYLIPRMIPLLLPTLVSAVASYVFLEASLAVLGLG
                     DPVLPTWGKIVNDAFTNGALYKGYYYWILEPAVLLMITGLGFAGLGFALDRIFNPRLR
                     GV"
BASE COUNT         1017 a         1324 c         1338 g         1009 t
ORIGIN      
        1 attcttgagg gtatccgtac agcaatgata actgtgggat taacccgctt cagtctgtgt
       61 gaaatcgctt caagcctgtc ttttgagcca gctcgacgac cgaccggtat tctgcctggc
      121 tgacaggtct gcgcagcctg gggtacaggc gggcattgaa cgccgggtga tactggtcca
      181 taaggtttag ataagtatcc ggggagatat cagccgcgat aaagtggagg atctcagcgc
      241 tgcctgccaa atcgccgggc agcaccaggt ggcggatgag caagccccgc cgggctaatc
      301 cgtgctcatc cagcatcaga tcgcctacct ggcggtacat ctcgcgcacc gcggcctggt
      361 ttacctgggg ataattgggg attttagaat agtatttagc aatcgcggca ctgctgtatt
      421 tcatgtccgg catgtagata tcgatcaccc catccagcag gtgcagtgcg tccagagaat
      481 cgtagccgcc ggtgttatag accaggggta gattcagacc ctgccgggcg gcaatcagca
      541 cagccgccag aatttgcgtc acgacatgcg agggcgagac caggttgata ttgtggcagc
      601 cttgcgcttg cagctctaaa aagatacctg ccagggcttc gggagacata ggctcgccgg
      661 gttggagctg gctgatctcg gcattctggc agtactggca gcgcaggttg cagcgggcaa
      721 agaataccgt gccagagccg cccgagccgc tgatcgggcg ttcttcaccg ggatgcgcgc
      781 cgtagctgct cacttcggcg agcagacccg tcttacaggc gccggtcttg cctgccagcc
      841 ggtttgcccg gcatgcccag gcgcactggc ggcattccgc cagcccggcg tgggcgctgg
      901 cggcgcgttt ttccagctcg ccgctgtgca gcaggctcag gtaggcaggc tctaaaggtt
      961 ccattacggg attatatcgg agaaatgcac ctggggatgg gctgacccgc cattgaagac
     1021 cggtacccac tctggaaatt catgcgtgcc agttaaagcc cgggtgggca tcgccgggcc
     1081 gctcctgcca acaaaatcta atccgggcga gatttatttg cgccggttga cttttacgta
     1141 ggtaaagagg cccaagaaag ccataaacat cccgatcaga gatgcagcgt aggaaataaa
     1201 gctcagcgcg aagctggcgg tgagcaattt gaccaccatc attagcggca ggactacccc
     1261 gaggatcatc aggagggtcc caatcaccat cagcttaagc gggctaagac tcattttcct
     1321 taaataacca gcaggctgcc aggtgttctg gctcgatatt gaattccggc gggcgttcca
     1381 catcgcatag tccgggcatc atgaatttac agcgcgggtg gaagcggcat ccgctgggcg
     1441 gcttcaccag gctgggcggc tcgcccgggg gaacttcctg aaattcatct acgtttttag
     1501 cgtcagggtc agaggttgcc gccagcaggg ccagggtgta ggggtgcttg ggattgcgga
     1561 tcaggtcgtc tacctgggcc ttttcgatga tttcgccggc atacatgacg aagatatact
     1621 ccgagaagta acggactgta gaaaggtcgt gggtgatgta aattactgcc aggtcgtgat
     1681 tttcctgcag gccgcgcaat aatctcagga tctcgacccg caccgaggcg tccagcatgg
     1741 ataccggctc gtctgcgacc agcagcttgg ggttgaggat catcgcccga gcaataacga
     1801 cccgctgctg ctgcccaccg ctgatcatat gcgggaattt gggcagaaat tcatccacgg
     1861 gggtcatctt gacttcttcc agcgctttgg tgatgcgctc aaggcgctcc gttttatcct
     1921 tgacgccgtt gatgatcagc ggctcttcca ggatttgctg gatgttcata aacggcggca
     1981 gcgccccata gggatcctgc tgcacatagc ccacctgcga ccggtaccag cgcatatcct
     2041 ggctgctgta ttccgacagt ttcttcccgt tatagacgat atcgcccttg gtaatcttat
     2101 tcagacccag gatggttttc atcaggctgg atttaccgca gccgctttcg cccaccaccg
     2161 cgactgcctc gccgtgatcc aggttgaagc tgactccatc cacagcgcgc acatagccgg
     2221 cgtgaccgaa gccgaaacgg cgtaattcga accagacgtg caaatcctgg acggagagca
     2281 gcgggaaatc tgcgccggga gcaggtattg tttgaatttt atcggtgact tgaagggtgt
     2341 cagacataaa taattgctcc ctgcgctagg tgtacaacca gcatttgacc aggcgcccgt
     2401 cctgggaaat cgttggtggg tcctggacgc atttctcgaa caccctcggg cagcgggctg
     2461 caaagcgaca gccagtcggt ggattgatca ggctgggcgg ctgcccgctg atgaaatcca
     2521 ggttggcggt gctgcgcagg cgcggcaccg aggacatcaa tgccgctgaa tagggatgca
     2581 gggggcgggt aaagaagcgg tggttgtcgc tcacttccac gatctgcccg gcgtacatca
     2641 ccgccacccg atctgccagc tcgctggagg tggagatatc gtgggtgatc aggatgaaac
     2701 tggtagcgat ctcttttttg acccgcttga gcagattcat gatgttagcc tgggtcagca
     2761 cgtccagcgc tgaggttggc tcgtccagga tcaccagatc gggcacggca accagcgaca
     2821 tggcgatcgc cacccgctgg cgcatcccac cgctcagctc gaacgcatag cgatcgacaa
     2881 aatcaaccgg cacgcccact cgctggaaca tggttttggc gtgatccagg gcggcttcct
     2941 tgctcacgcc gtggtgcacc atcatcggct cggccacctg gtcgcccacc cgcagcaccg
     3001 gattaagcga attcattgcc gcctggggca ccattgccat cttcacccag cggacgtccc
     3061 ggcggaattt ctcctcgtcc agtttcataa tatcgacgcc gtccaggaaa acttcccccg
     3121 aatacgtccc tacattgcgg ggaagcaggc gcagcagtgc tttggccatc gagctttttc
     3181 cgcagcccga ttcgccgata atcacaatcg cttcattggg cgccagggtt aaatccacgc
     3241 catctacagc ctgcaccaac ccccgcgcag ctttaaaata cagtttcagg tttttgacct
     3301 gcagtaatgc tttttcagcc ataccagtta gacccctcgt aagcgtggat tgaagatgcg
     3361 gtccaatgcg aagcccaacc ccgcaaagcc cagcccggtg atcatcagca gcaccgccgg
     3421 ttccaggatc cagtaataat agcctttata cagcgcccca ttggtgaaag catcgttaac
     3481 gatcttgccc caggtgggca gcaccggatc gcccaggcca agcactgcca gggaggcttc
     3541 caggaacacg tacgacgcca ccgctgaaac cagggtgggg aggagcaagg ggatcatgcg
     3601 cgggatcaaa tacttgaaga tcacccgccc atcgctggcg ccgtacgctt tggcggcttc
     3661 gatgtaggtg gattctttca cctgcatgaa gatggcgcgg taggttttga tcgagccggt
     3721 aaagatactt aaggcgatcg ttgcccccag gatggtccag atactgcggt cgacaaatgt
     3781 gccgatcatg atcaagatgg gcaggaaagg cagtaccaga ttgacttcgg tgatgcgctg
     3841 gattagctcg tcgacccagc cgctgtacca ggtgccgatg gcggcgatga tcatggtcaa
     3901 caccgatgta cccgccgcag ctaaaaggcc aaacgccagc gctatcggcg caccccacag
     3961 cagtgccacg ctcagatcgc gccgggcatg gtcggtgccg gcaattccaa acaattgtcc
     4021 gtggaagacc agctcagcgt tgatatccga gccctgctcg aaggtcagcc cgttgatcac
     4081 cagctcgtaa gtgcctttga gcggggtgag cggatcggat tcgggcaccg aaaacaagcc
     4141 tttcatcgtt accacgccct tgaggcgccg caccagtttc tcgtcttgtg aaaaccggta
     4201 ggtttgcttt ctactcaccc caaaatcgct gatcctgatc tcacgcccgt cgggggtatt
     4261 gagatagatc gagacaaacg gctgtttggc ctcataggtt gtgttaaagt acagcaggat
     4321 ctcctgcgga taatcatcgt agttgaattc gatcggataa ctgaaattga tcgtcgtcgt
     4381 gttctgctcg cccggaatga cttccttggt aaatccttct tcaccgcttt tgacggcgaa
     4441 agattctggc aaatctctgg cggtgaaata attgaaccag gccgggggga cattcttggg
     4501 attttgatac cagatttctt caccgccgcg ccataaacgg atggcttcat tgtatgggat
     4561 ggtcaccaca gcataaacag ccagagatac cagcgcgagc acgatgatca ttccaaatat
     4621 cgccgaggga tactgcgcaa tatcccgcaa ggtctttttg agcgagttca tggctaactc
     4681 cccccaac
//
LOCUS       DATMXK010000174         6484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_167118, whole genome shotgun sequence.
ACCESSION   DATMXK010000174 DATMXK010000000
VERSION     DATMXK010000174.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167118"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            82..528
                     /locus_tag="VN363_03225"
     CDS             82..528
                     /locus_tag="VN363_03225"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin B12-binding domain-containing protein"
                     /protein_id="HWQ83550.1"
                     /translation="MSEPKSSVNEKKIRVVVAKPGLDGHDRGAKVVARALRDAGMEVI
                     YTGLRQTPEMIAETALQEDADVIGLSVLSGAHMALVPRILELLRQNGQEYVKVFLGGI
                     IPDEDVPRLIEMGVVGVYGPGTITEAIIKDVRQAVLGPSDAGTHPV"
     gene            546..1562
                     /gene="meaB"
                     /locus_tag="VN363_03230"
     CDS             546..1562
                     /gene="meaB"
                     /locus_tag="VN363_03230"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559688.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016772 - transferase activity,
                     transferring phosphorus-containing groups [Evidence IEA];
                     GO_process: GO:0015819 - lysine transport [Evidence IEA];
                     GO_process: GO:0015822 - ornithine transport [Evidence
                     IEA];
                     GO_process: GO:1903826 - L-arginine transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl Co-A mutase-associated GTPase
                     MeaB"
                     /protein_id="HWQ83551.1"
                     /translation="MSLVESVLSGNRLALARLLTQIENGLPEGWAALNELYPHTGKSH
                     RIGVTGAPGTGKSSLVNQLARYYRQGGSFTPRRVGIIAVDPSSPFTGGAILGDRVRMR
                     DLAGDPGVFIRSMATRGTLGGLAAATAGMVQVLDAAGFDLIIVETVGAGQSEVDIARL
                     AHTTVVVEAPGLGDEIQAIKAGILEIADILVINKADRPGVEAAERALKNNLQLAHNTP
                     RSFLHHGTRELLPELVQEDKGGSIWTPPVLKVVATEGDGIRELVEAIEQHRKFLEISG
                     ERQRRDLARVQSELENILRETLVARWRAASNGEQYRAVLSRMAARQISPWQAVQQLLD
                     GGEG"
     gene            1559..2101
                     /locus_tag="VN363_03235"
     CDS             1559..2101
                     /locus_tag="VN363_03235"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWQ83552.1"
                     /translation="MIQGKRVRLRAPTRTDLPQFVAWLNDSEVIAGLLVSIPMSLEDE
                     EAWFAGMLNRPLAEHPMVIEIQREEAWIAVGNCGFHNIDWRARAAEVGIFIGEKSLWN
                     QGYGSEAMRLLLAHGFSTLNLNRIALDVYETNPRAIRSYEKVGFVHEGRKRQAMFKDG
                     KYFDILIMSVLRAEWKEDQD"
     gene            2098..3090
                     /locus_tag="VN363_03240"
     CDS             2098..3090
                     /locus_tag="VN363_03240"
                     /EC_number="2.7.6.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559685.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004749 - ribose phosphate diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0009156 - ribonucleoside monophosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribose-phosphate diphosphokinase"
                     /protein_id="HWQ83553.1"
                     /translation="MSSHHTYRDCGMYGDIQLFAGTGSPDLAQAIATYLGIPLSGRDI
                     VQFPNENLFVRLHQSVRGQDVYVVQSTSSPVHRNLMELLIMLQCLRLDSAARVTAVIP
                     YLAYARSDKKDRPRVPITARLVADMVEVAGADRYMTLDLHAGQIQGFFSIPGDVLTAF
                     HILTEYVEALLPTLNDPVVVAADLGFAKKGRNFAAQIGAPIAFVEKRRSGIGAQAEAL
                     SIIGDVAGRDVILVDDEVDTAGSMVQAVQLVKREGARRVYMVFVHPIFSGPAAERLGQ
                     LPVEEMITTDTVQVSAEKRALMGEKLKILSVASLLGEVILRAHEGRSVGEMFHE"
     gene            3097..3921
                     /gene="mutM"
                     /locus_tag="VN363_03245"
     CDS             3097..3921
                     /gene="mutM"
                     /locus_tag="VN363_03245"
                     /EC_number="3.2.2.23"
                     /inference="COORDINATES: protein motif:HMM:TIGR00577.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008534 - oxidized purine nucleobase
                     lesion DNA N-glycosylase activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-formamidopyrimidine glycosylase"
                     /protein_id="HWQ83554.1"
                     /translation="MPELPEVETIARLLRQGAEDQPGILGRKITAVRVLWARSVAEPD
                     ADEFLARLTGQEIQQIDRRGKFLVITLPQETLLIHLRMSGDLFVEAQEQQLAPHHRVV
                     LDFDNGWRLAFNDPRKFGRMWLVSDPQQVLGGLGPEPFDRSLDDEGLYNRLNAFRRQL
                     KPLLLDQHFMAGMGNIYTDEALHLAGLHPLRLSSSVTRDQSLQLLRSIRQVLQEGIDR
                     HGTSIDWVYRGGDYQRYLRVYQRTGEPCPVCGTPVERFVVGQRSTHYCPSCQALSV"
     gene            3940..4614
                     /locus_tag="VN363_03250"
     CDS             3940..4614
                     /locus_tag="VN363_03250"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83555.1"
                     /translation="MTDSQLLLIVATLALVIGAAIGSLISSLRNTSSQKSPQRPPEKN
                     KPALLQVWRDQTRGRLIVGIKGEQAKAVSELSPDTLEILHKTLVELGAWVGIQPEAAP
                     APAATPLAEPEKELSSLLSPVNESKAARLGPVDLFARAIRADVHKPEPPAASIAAQID
                     AILQEKLAADPTITRAVRLLEIPAKGMVVMVGLDQYAGVDEVPDPTVRALIHEAVEEW
                     ERRTGM"
     gene            complement(4627..>6484)
                     /locus_tag="VN363_03255"
     CDS             complement(4627..>6484)
                     /locus_tag="VN363_03255"
                     /inference="COORDINATES: protein motif:HMM:NF019155.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83556.1"
                     /translation="MRLFGGAQADNLMQRLKIDDALPLEVGLVSRIVEQSQTRVEGSN
                     FDVRKHLLEYDDVLNTQRAKIYGQRNRIFTKDDLSEDVSEMLHAEVNRRVPLALEDEE
                     GPWKLLSWLDQIQPPLNIGGFLFPSYTLKLLVDAVINTAKATGDYDENATAIPVQTGR
                     QILLQVMQAAIASEQNHLTATVSTLHSQSEDRLKQQIGERQELLDTFLEGVELGDETD
                     TRKPVDLANELSGMLHLPLRLTPDQQRALRDQPRSARAVVAEQTEALLTQQAILRLVG
                     AVERRLEESLELDSASLAQEDWDTIGERILEGIHKVFERRQERFLGEQGSIIKDLDSA
                     LARLNNPALQRGHLTYLLVVLTEGQRASFDKKTHRRVWTRTTRLLYTYYAAQLIKDRD
                     PEEITTDVLAHLEKAEETIRRSMGIAELQRLGGQSLNSLDESLQATIQRVLGEEEAQK
                     FANTLINSLPADLQESLVEPLGRRVLTNNYRGLLLGVITELWVDYLTQMEALRVSIGL
                     EAYAQRDPLVQYKSRAYELFQVLLDNMRLGVVSRMFTYRPRQASPQTTWDDTNGSSPA
                     DDSGPQPEATEEENGEGEAEGTDEADEAALVIAQQSADRSTSTAKKRRRRRR"
BASE COUNT         1339 a         1745 c         1940 g         1460 t
ORIGIN      
        1 cctgaatcgg tgccatcggc tgagcctgga taaacaagtg cgtgtataat ctgattatct
       61 tcaaacgatg ggaaggtctg catgagcgaa cccaaatcaa gcgtgaatga aaagaaaatt
      121 cgcgtcgttg tggcgaaacc tggtctggat ggtcacgatc ggggtgcaaa agtggtcgcc
      181 cgggcgttac gagatgccgg gatggaggtg atctacaccg gattacgcca gacccctgaa
      241 atgatcgccg aaacagcctt gcaggaagat gcggatgtca tcggtctctc ggtgctatcg
      301 ggtgcccata tggccctggt gccgcgcatc ctggaattat tacgccaaaa cggacaggag
      361 tatgtcaaag tgttcctggg cgggattatt ccagatgaag atgttccgcg cctgattgaa
      421 atgggtgtgg tgggtgtcta tggcccaggg accattaccg aggcgatcat taaggatgtt
      481 cgccaggctg tattgggccc cagtgacgct ggaacccatc ccgtctgagc ttgactctca
      541 gggttatgtc cttagttgaa tctgtattgt caggcaaccg cctggcttta gcccggctgc
      601 taacgcagat agagaatggg ctgcccgaag gttgggcagc cttaaatgag ctttacccac
      661 acaccggcaa atcccaccgg attggggtga ccggcgcgcc cggcaccggt aaatccagcc
      721 tggtcaacca gctggcgcgc tattaccgcc agggtggcag ctttacacct cgccgggtag
      781 gtatcattgc ggtcgatccg tccagccctt tcaccggcgg ggcaatcctg ggggatcgcg
      841 tgcgcatgcg tgatctggca ggtgatccgg gggtctttat ccgctcgatg gccacgcgcg
      901 gtacccttgg cggactggct gctgcgaccg ccgggatggt gcaggtgctg gatgcggctg
      961 gtttcgactt aattattgta gaaaccgttg gagcagggca aagcgaagtc gatatcgctc
     1021 gcctggcgca taccacagtc gtggtcgaag cgcccgggct gggagatgag atccaggcga
     1081 tcaaagccgg gattttagag atcgcagata tcctggtgat aaacaaagcc gatcgccctg
     1141 gtgttgaagc agctgagcgt gcgcttaaga ataatttaca gcttgcccat aacacccccc
     1201 gcagcttttt gcatcatggc acacgcgagc ttctaccaga gctggtgcaa gaagataaag
     1261 ggggcagtat ctggaccccg ccagtcttga aggttgtggc tacggaaggc gatgggatta
     1321 gagaattggt cgaagcgatc gagcagcacc gtaaattcct ggagatctca ggcgagcgcc
     1381 agaggcgtga cctggcgcgc gtccaatccg aattagagaa catactgcgt gagacgctgg
     1441 tcgcccgctg gcgggctgct tcgaatggcg aacagtatcg agcagttttg tccaggatgg
     1501 cagcccgcca gatatccccc tggcaggctg tccagcagct gttggatgga ggcgaaggat
     1561 gattcaggga aaacgcgttc gcttacgggc cccaactcga acagacctgc cgcagtttgt
     1621 ggcctggctg aacgattcgg aggtaatagc ggggctcctg gtctccatac cgatgtccct
     1681 ggaagatgag gaagcctggt tcgcaggcat gctcaaccgc ccgcttgctg aacatccgat
     1741 ggttatcgag attcagcggg aggaagcctg gatcgccgtg ggtaactgtg ggttccataa
     1801 tatcgattgg cgggcgcgag ccgctgaagt aggtatattt atcggggaga aatccctgtg
     1861 gaatcagggt tatggcagtg aggcaatgcg cctgctcctg gcgcatggct tcagtacttt
     1921 gaacttgaac cggattgctc tggatgtcta tgaaaccaat ccgcgggcga tccggtcata
     1981 cgagaaagtg ggctttgtgc atgaggggcg caaacgccag gccatgttta aggatggcaa
     2041 atatttcgac atcttgatca tgagcgtatt gcgagcggaa tggaaggagg accaggattg
     2101 agcagccacc acacctaccg ggattgtgga atgtacgggg atatccagtt atttgcgggg
     2161 actggctccc cagacctggc gcaggccatt gccacctacc tgggcatccc gctcagcggt
     2221 agagatatcg ttcagttccc caatgagaac ctgtttgtcc gcctgcacca gagtgtccga
     2281 ggccaggatg tatatgtggt ccagtcgacc agctcgcctg tgcatcgcaa cctgatggag
     2341 cttttaatca tgctgcagtg cttgcgcctg gattcggccg cccgggtcac ggcagtgatc
     2401 ccatatctgg cttatgctcg ctcagacaag aaggaccggc cgcgagttcc gatcaccgcc
     2461 cgcctggtag cagatatggt tgaggtcgcg ggagcagatc gctacatgac gttagatttg
     2521 catgccgggc aaatccaggg cttcttttcg atcccggggg atgtgctgac agcctttcat
     2581 attttaacgg agtatgttga agccttactt cccaccttga acgacccggt ggttgtggct
     2641 gctgatttgg gttttgccaa aaaagggcgc aattttgcag ctcagattgg tgcacctatt
     2701 gcgtttgttg aaaagcgccg ctcaggtata ggcgcccagg ctgaagctct gtcgatcatc
     2761 ggggatgtgg ccggccggga tgtgattctt gtggatgatg aggttgatac cgctggctcg
     2821 atggtccagg ctgtccagct cgtcaagcgc gaaggagccc ggcgggtgta tatggtattt
     2881 gtacacccca tcttctctgg gccggcggca gagcgcctgg gccagctgcc agttgaagag
     2941 atgatcacca ccgataccgt gcaggtctcc gcagaaaagc gggccttgat gggcgaaaaa
     3001 ttaaagatcc tttcggtagc ttctctgttg ggtgaagtaa ttctgcgtgc tcatgagggc
     3061 cgcagcgtgg gtgagatgtt ccacgaatag taaaacatgc cagagctgcc tgaagttgaa
     3121 acgattgccc ggttgttgcg gcaaggtgca gaggatcaac ccggaattct gggacgtaag
     3181 atcactgccg tccgggttct gtgggcgcgc agcgtggctg agccggacgc tgatgaattt
     3241 ctggcgcgtc tgaccgggca ggaaatccag caaatcgacc ggcggggtaa atttcttgta
     3301 atcacactcc cacaggaaac actcctcatt cacctgagaa tgagcggcga tcttttcgtg
     3361 gaggcgcaag aacaacagct ggcgccccac caccgggtgg tgctggattt tgataacggc
     3421 tggcgtttgg cgtttaatga tccccgcaaa ttcggccgga tgtggctggt aagcgatccc
     3481 cagcaagtat tgggcggtct gggacctgaa ccttttgata ggtcgctgga tgatgagggg
     3541 ctttataatc gattaaatgc ctttcggcgg cagttaaaac ccttattgct tgaccagcac
     3601 tttatggctg ggatgggcaa tatctatacg gatgaagcgc tgcaccttgc tgggctgcat
     3661 cccctgaggc tttccagctc ggtcacccgg gatcaaagcc tgcagctgct gcgaagtatt
     3721 cgccaggtgc tgcaagaagg gattgaccgg catgggacga gcatcgattg ggtatatcgc
     3781 gggggagatt accagcgata tttgagggtt tatcaacgaa ccggggagcc ctgcccggtt
     3841 tgtgggacgc cggtcgaacg ttttgtcgta gggcagcgca gcacccatta ctgcccgtcc
     3901 tgccaggccc tgtctgttta agactcggga gggattagaa tgacggattc acagctcctg
     3961 ttaattgtcg ccacccttgc cctggttatt ggggccgcaa tcgggtcatt aatatcttcc
     4021 ttgcgaaata ccagctccca aaaatctccc cagcgtcccc ccgagaaaaa caagcctgcg
     4081 cttttgcagg tatggcgtga ccagactcgc ggtcgcctga ttgttgggat caaaggtgag
     4141 caggcgaaag ctgtcagtga gctttcgccg gatactctgg agatcctgca caaaacgctg
     4201 gttgagctgg gcgcctgggt cggcatacag ccagaagcag ctccagctcc ggcggccacc
     4261 ccgctcgccg agccagaaaa ggaactttcc tccttattat cacccgtcaa tgaaagcaag
     4321 gcagctcgcc tgggaccggt agatctgttc gcaagggcta tacgggctga cgtgcacaaa
     4381 cctgagccgc ctgccgccag catcgcagcc cagattgatg cgatcctcca ggaaaaatta
     4441 gctgcagatc caacgataac ccgagcagtg cgccttttag aaatccccgc caaaggcatg
     4501 gtggtgatgg tcggccttga ccagtacgca ggtgtggacg aagttcccga ccccaccgtg
     4561 cgcgccttga ttcacgaagc ggttgaggaa tgggaacggc gcacaggaat gtaattgcga
     4621 gtctggttac ctgcgccgcc gccggcgttt ctttgcagtg gaagtgctgc ggtctgcgga
     4681 ttgttgagca attaccagag cagcctcatc agcctcatcc gtgccctccg cctcgccctc
     4741 accgttttct tcttcagttg cctctggctg cgggcctgaa tcgtctgctg gtgagcttcc
     4801 attcgtgtca tcccaggttg tctggggcga agcctgccgc gggcggtagg tgaacatccg
     4861 gcttacgacg cccaatcgca tattgtccag cagcacctgg aaaagctcat aggcacggga
     4921 tttgtactgc accagcgggt cgcgctgcgc ataggcttcc aatccgatcg agacccttaa
     4981 tgcctccatc tgagtcagat aatcaaccca cagttcggtg atcaccccca ggagtaagcc
     5041 gcgatagttg ttggtcaaga cgcgcctgcc gagtggttct accaggctct cctggagatc
     5101 agctggcagg ctgttgatca gcgtattcgc gaatttttgg gcttcttcct cgcccagcac
     5161 ccgctggatc gttgcctgca ggctttcgtc cagactgttg aggctttgcc cgcccaggcg
     5221 ctgcagctcg gcgatcccca tggagcggcg gatcgtttct tcggcttttt ccaggtgggc
     5281 cagcacgtcc gtggtgatct cttctggatc gcgatctttg atcaattggg ctgcatagta
     5341 tgtgtacagc agccgcgtgg tgcgcgtcca gacccgccgg tgagttttct tgtcgaagct
     5401 ggcacgctgg ccttccgtaa gcacaaccag caagtaagtg agatgcccac gctggagggc
     5461 cgggttgttc aaccgcgcta aagcgctgtc gaggtcttta atgatcgaac cctgttcgcc
     5521 caggaagcgt tcctggcgcc gttcgaatac cttgtggatg ccctcgagga tcctttcacc
     5581 aatcgtatcc cagtcttcct gggccagact ggcagaatca agctccaggc tctcctccag
     5641 gcgccgctca acggccccaa ccagacgcag gatggcctgt tgggtcaaca gggcttcggt
     5701 ctgttcagct accactgccc tcgccgagcg cggctggtcg cgcagggcgc gctgctgatc
     5761 cggtgtcagg cggagcggca ggtgcagcat accagacagc tcattcgcca ggtcaaccgg
     5821 cttgcgggta tcggtttcgt cgcccagctc tacaccctct agaaaagtgt ccagcaattc
     5881 ctggcgctcg ccaatttgct gcttgagacg atcttcgctc tggctgtgca aagtgctgac
     5941 cgtcgcggtc agatgatttt gctcgctggc aattgcagcc tgcatcacct ggagcaagat
     6001 ttgcctgcca gtctggactg gaatggctgt ggcgttttcg tcataatccc cggtggcttt
     6061 ggcggtgttg atgacggcat ccaccaatag cttcaacgta taggatggga acaggaaacc
     6121 cccaatattc agcgggggct ggatttgatc cagccaggag agcagcttcc agggtccctc
     6181 ctcatcctcc agcgccaggg gcacgcggcg attaacttcc gcgtgcagca tctccgatac
     6241 atcttcgctc agatcatctt tggtaaagat ccggttgcgc tggccgtaaa tcttggcgcg
     6301 ttgggtgttc aagacatcat cgtattccag caggtgttta cggacgtcaa aattggaacc
     6361 ctcgacccgg gtctgggatt gctcgacaat ccgtgagacc aatccaactt ccaggggtag
     6421 ggcatcatcg atcttgaggc gctgcatcaa attgtcggcc tgggccccgc cgaataatcg
     6481 catc
//
LOCUS       DATMXK010000175         4369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_25084, whole genome shotgun sequence.
ACCESSION   DATMXK010000175 DATMXK010000000
VERSION     DATMXK010000175.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_25084"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..642
                     /locus_tag="VN363_03260"
     CDS             <1..642
                     /locus_tag="VN363_03260"
                     /inference="COORDINATES: protein motif:HMM:NF024732.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA hydrolase/transferase C-terminal
                     domain-containing protein"
                     /protein_id="HWQ83557.1"
                     /translation="AVLKYLYDKRDLGIHTELFSDGVIGLVEAGVLTNARKKINPGKI
                     TAGFILGSRRLYAWVDDNPLIELHPTEYMNDPYIIAQNDRQVSLNSAIEVDLTGQVCA
                     DSIGPKLYSGVGGQLDFIYGAARSKEGVPIIALPSTATLRNGQMISRIASMLKPGAGV
                     VTTRNHVRYVVTEYGIADLYGKTIRQRAHALIQIAHPDFQDQLMHEAHALNYV"
     gene            660..2144
                     /gene="pyk"
                     /locus_tag="VN363_03265"
     CDS             660..2144
                     /gene="pyk"
                     /locus_tag="VN363_03265"
                     /EC_number="2.7.1.40"
                     /inference="COORDINATES: protein motif:HMM:NF004978.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004743 - pyruvate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyruvate kinase"
                     /protein_id="HWQ83558.1"
                     /translation="MAILSDRQKQPLSERYARIVATIGPASQDQAKLLQLIKAGMDVA
                     RLNFSHGTHDYHLETIKNLRAASAEAKKPVTILQDLQGPKIRTGELENGAVQLKAGDR
                     LFLTSSKLTGNQGIVSVDFPRLTEAVKPGGRILLDDGNLELVVVSVSHEKVETQVVLG
                     GTLLPHKGVNLPRAHLDISPVTEKDLEDLAFGLKHGVDAVALSFVRSPKDINDLRKVI
                     QTLAEAGDNVPVIAKLERPEALDALDSIVIAADGVMVARGDLGVEMAPETVPIAQKTI
                     IDCANKHARLVITATQMLDSMIHNPRPTRAEASDVANAIFDGSDAVMLSGETAAGKYP
                     VQAVETMDAIIRQAEAKTSEWGHWRGLDVNDFVANDTYYITRGARELACDRDVSAIAI
                     FSKTGRSALMMSKNRPQVPIFGFTPVESTYHRMNLLWGVTPFLVKNVDSLEDMLKEID
                     ITFADSGMVQPGQQVVMVCGYPVSEQRPANLALLYTVGQNLRFP"
     gene            2165..>4369
                     /locus_tag="VN363_03270"
     CDS             2165..>4369
                     /locus_tag="VN363_03270"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83559.1"
                     /translation="MTDANVWIPGSLPDIPGPLQRFLPVVPKGVISNWLTANVNPGSL
                     ILDPFGSTPLPAVEAARAGYRVLVAANNPVARFLLDMAADPPAEIELKSALAELSRAM
                     KGSERLPQHISNLYKTECAHCGATITAEAFLWERGANAPYARVYTCEKCGDSGEHPAL
                     DSDVERARAHQKHNLTWSRALERVAPLNDPDREHVEEALEAYLPRAVYALMTLVNAID
                     LVAPSPAATAERAAWIYRQRSIIALLLSACDRGNCLWPIGGSRTRPRQLTRLGRFIEF
                     NLWKVLEESATQWSTEASGGGQVPLVLWPQLPPAEGGITVFDGRIRELIEQLSTQPVN
                     AMISAIPRPNQAFWSLSALWSGWLWGREASAPFKSVLRRRRYDWAWHTTALCSALQNI
                     ESCLLDDSPFFTLVSEVEAGYLTALFIAAGLSGFDLLGLALRPESNQLQAHWAIQSSA
                     VHSTMDGNQAGTLPSSQKIQDAAVSASLKYLRERAEPAGISAIHTAALINAQDMIGAF
                     PREPNQAAELYTSLHNGMQQAYLNRSEFQRFGGSEGSLEGGKWWIGGSKLNQFSDPAI
                     LPIADRVEMELMRAFHRQQTWEFSALDHHLCAVFPGLDTPDMSLIRACLESYATEENP
                     GVWTLRTGESTAGRRNDLGEMRELLDQVMARAGLTTQTPVVRLPEGQFGLIYENPEGV
                     AVYQFQIMVSAVLGLVMNSQIKAIRGAYILYPGSRAVLMDYKIHNNPVLNHQI"
BASE COUNT         1031 a         1168 c         1197 g          973 t
ORIGIN      
        1 gctgtattaa aatacttata cgataagcgc gatctcggga ttcataccga gttgttttca
       61 gacggcgtga ttggtctggt cgaagcaggt gtattgacaa atgcccgcaa gaaaattaac
      121 cccggcaaga taactgccgg atttatcctg ggaagccggc gcctgtatgc atgggttgat
      181 gacaatccac tgatcgagct gcatcctaca gaatacatga atgaccccta tatcatcgct
      241 cagaatgatc gccaggtatc cctcaattcc gcgattgaag ttgatctaac cggacaggta
      301 tgcgcagaca gcatcggacc caaactatac agtggagtag gtgggcagct agattttatt
      361 tacggtgcag cgcgctcaaa agaaggggtt ccaataatcg ccctgcccag tacggcaacc
      421 ctgcggaatg gtcaaatgat cagccggatc gcctcgatgc ttaaacctgg tgcaggtgta
      481 gttacaaccc ggaaccacgt tcgttatgta gtcaccgaat atgggatagc cgatctgtac
      541 gggaagacta ttcgccagcg agctcacgcc ctcatccaga tcgcccatcc cgacttccaa
      601 gaccagctga tgcacgaagc gcatgcgctg aattatgtgt aattaatggg gagacccaga
      661 tggcaatctt aagcgaccgg caaaaacaac ccttatccga acgttatgcg cgcatcgtag
      721 ccaccatcgg cccagccagc caggatcaag cgaagctgct ccaattaatc aaggcaggaa
      781 tggatgtggc gcggttgaat ttttcccacg gcacccatga ttaccatctg gaaacgataa
      841 agaacctgcg cgccgcctct gctgaagcca aaaaacccgt cacgatccta caggatttac
      901 aaggtcccaa gattcgcacc ggggagctcg aaaatggcgc ggtgcaattg aaagccggtg
      961 atcgtttgtt tctgacgagc tccaaactga caggcaatca ggggattgtc tcagtggatt
     1021 ttccgaggtt aaccgaggca gtgaaacccg gcgggcggat cctcctggat gatggcaatc
     1081 tggagctggt agtggtttca gtctcccatg agaaggttga aacacaggtt gtcctgggcg
     1141 gcaccttact gccccataaa ggggtaaatc tccctcgagc ccatctggat atttcaccgg
     1201 tcaccgaaaa agatctggaa gatctggcct tcgggcttaa gcatggtgtc gatgcagtag
     1261 cattatcttt tgtgcgctca ccaaaagata tcaatgatct ccggaaggta atccaaacac
     1321 tcgcggaagc aggagacaat gtccctgtga ttgccaagct ggaaaggcca gaggcactcg
     1381 acgcgctgga ttcgattgtt atagcagccg atggtgttat ggtggcacgg ggtgatctgg
     1441 gggtagaaat ggcccccgaa actgtgccga tcgcccagaa gacgatcatt gactgcgcca
     1501 ataagcacgc ccgcctggtt atcactgcta cccaaatgct ggactcaatg atccataatc
     1561 cgcgtccgac gcgcgcagaa gcatctgatg ttgccaatgc aatttttgat ggcagcgatg
     1621 ctgtaatgct ttccggggaa actgccgcag gcaagtatcc ggttcaagcc gttgaaacca
     1681 tggatgccat catccggcaa gccgaagcaa aaacttctga atggggacac tggcgcgggc
     1741 tggatgtcaa tgatttcgtc gcgaatgata cctattacat tacgcggggt gcgcgcgagc
     1801 tggcctgtga ccgggatgtg tccgcaattg ccatattcag caagaccggg cgaagtgcat
     1861 tgatgatgtc taaaaaccgc ccccaggtgc ctatcttcgg ttttacacca gttgaatcca
     1921 cctaccaccg gatgaattta ctttggggtg ttaccccatt cctggtgaaa aacgtggata
     1981 gtctggaaga tatgctaaaa gagatcgata ttacgtttgc agactcgggg atggtccaac
     2041 ccgggcagca agtcgtcatg gtttgtggtt atccggtgag cgagcagcgc ccggcaaatc
     2101 tggctttgct ctataccgtc gggcagaatt tgcgctttcc ttgagctgaa tctggagaca
     2161 gcccatgaca gacgcgaatg tatggatacc gggcagtcta cctgacattc ctggcccact
     2221 gcagcggttc ctacccgtgg ttccaaaagg tgtcatcagt aactggctga ctgcaaatgt
     2281 caatccgggc agcctgatcc ttgacccgtt cggcagcaca ccattacccg cggttgaagc
     2341 tgcccgggct ggctaccggg tattggtcgc cgccaacaat ccggtggcgc gctttttact
     2401 ggatatggct gccgaccctc cggcagaaat tgagcttaag tctgcgctgg cggagctgag
     2461 ccgggcgatg aaaggcagcg aacggcttcc ccaacacatc tcaaatctgt ataaaactga
     2521 gtgcgcccac tgcggagcga cgatcactgc tgaggctttc ttatgggaac gcggcgcaaa
     2581 cgcaccttat gcacgcgttt acacctgcga aaagtgtgga gacagcggtg agcatcctgc
     2641 cctggatagt gatgttgagc gcgccagggc acatcaaaaa cataacctga cctggtcgcg
     2701 agctctggaa cgtgtggcgc ctttgaatga tcctgatcgg gagcacgtcg aagaagctct
     2761 ggaagcctat ttgcccaggg cagtctatgc cctgatgacc ctggtcaatg ccatcgacct
     2821 ggtggcgcca tcgccagcgg caaccgcgga acgcgcagcc tggatctacc gccagcggag
     2881 cataatagcc ctgctgctct cagcctgtga taggggcaat tgtctttggc caatcggtgg
     2941 ctcgcgcacc cgtccccgcc aactcacccg gcttggtcgt tttatcgagt ttaacctgtg
     3001 gaaagtcctc gaagagtctg cgactcaatg gtcaactgaa gcctcaggcg gcgggcaggt
     3061 ccccttagtg ctctggccgc agctcccccc tgcagaaggc gggatcaccg tattcgatgg
     3121 ccgcatccgc gagctgatcg agcaattatc cacccagcca gtcaatgcca tgatcagtgc
     3181 aatcccccgg cctaatcagg ccttctggag cttatctgcc ttatggtcgg ggtggttatg
     3241 gggtcgggag gcatctgctc cgttcaaaag tgttctgcgc cggcggcggt atgactgggc
     3301 atggcataca accgcattat gctcggccct ccaaaacatt gaatcctgtc tgctggatga
     3361 ctcacctttc ttcaccctgg tcagcgaggt tgaagccggt tatctcacag ctttgtttat
     3421 cgctgccggg ctttccggat ttgatctcct cggtctggcg ctgcgccctg aatccaacca
     3481 gctgcaagcg cactgggcaa tccagtcatc cgctgtacat tcaacaatgg atggtaatca
     3541 ggctggtacg ctccccagct ctcagaagat acaggatgct gccgtgagtg catccttgaa
     3601 atatctcagg gagcgcgccg agcccgcagg tatatccgcc atacacacgg ctgctttaat
     3661 taacgcacag gatatgatcg gagcattccc tcgcgagccc aaccaggcag cagagcttta
     3721 cacctccctc cacaatggca tgcagcaggc ttacctgaac cgcagtgagt ttcaacggtt
     3781 tggcgggagc gaagggtctt tggaaggtgg caagtggtgg atcggaggat caaaattgaa
     3841 ccagttcagc gatcctgcaa tcctccctat tgctgatcgg gtcgagatgg aattgatgcg
     3901 agctttccat cgccagcaaa cctgggagtt ttcagctctg gaccatcatc tgtgtgcagt
     3961 ctttccaggt ctcgacaccc cggacatgag cctgatccgg gcttgccttg aatcctatgc
     4021 cacggaagaa aacccgggcg tctggacatt gcggaccggg gagagcactg caggccggcg
     4081 aaatgatctg ggtgaaatgc gggaacttct agatcaggta atggcccggg ctgggctgac
     4141 cactcaaacc ccggttgtga gattacccga aggacagttc gggttaattt acgagaaccc
     4201 tgagggcgtc gcagtgtacc aatttcagat tatggtgtcg gcggtgctgg ggctcgtgat
     4261 gaatagccag ataaaagcga ttcgtggagc gtacatcctg tatccgggca gccgggcagt
     4321 gttaatggat tataaaatcc acaataatcc tgtcttaaat catcagatt
//
LOCUS       DATMXK010000176         4997 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_117940, whole genome shotgun sequence.
ACCESSION   DATMXK010000176 DATMXK010000000
VERSION     DATMXK010000176.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4997)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4997)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4997
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117940"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..303
                     /locus_tag="VN363_03275"
     CDS             <1..303
                     /locus_tag="VN363_03275"
                     /inference="COORDINATES: protein motif:HMM:NF024947.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SbcC/MukB-like Walker B domain-containing
                     protein"
                     /protein_id="HWQ83560.1"
                     /translation="QRDYEMFSGGEAFRVNFAIRLALSEILAQRAGARLQTLVIDEGF
                     GSQDALGRQRLIEAINMVREDFAKILVITHIDELKDAFPNRIEVEKTDRGSTVRVS"
     gene            317..1174
                     /locus_tag="VN363_03280"
     CDS             317..1174
                     /locus_tag="VN363_03280"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559817.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019213 - deacetylase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWQ83561.1"
                     /translation="MDNQKRVLLAVLAHPDDESFGMGGTLALYARQGVAVHLVCATRG
                     EAGEVDEEYLEGFGSIAERRESELRCAAEMLGLADVHFLDYRDSGMTGSPANQHPQAL
                     AAAPLLEVAEKIACWIRELQPQVVVTFDPIGGYRHPDHIAIHQATVKAFTMAADPQIW
                     AGGLAPFRTQKLYYHTIPRGLFRWGVRLLRLFGRDPRRWGRNGDIDLVALAADDFPVQ
                     ARIDIRPVAGIKAQASACHASQGGPVMRQGLFGWVTRLGNRYENYMLADPPPVPGLIE
                     NDFFADISS"
     gene            complement(1164..2057)
                     /locus_tag="VN363_03285"
     CDS             complement(1164..2057)
                     /locus_tag="VN363_03285"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="winged helix DNA-binding domain-containing
                     protein"
                     /protein_id="HWQ83562.1"
                     /translation="MPTHILRPTWHFVTPADIRWLLILTAPRVHAVNASMLRKLGLES
                     AVLKRSQTALAKILSGRQLTRTELRDALQQAGISILSGLHLAYILMSAELDGLICSGA
                     RRGKQFTYALLDERAPPVASLDRQTALTELSRRYFQSRDPATVQDFDRWSGLTLADAR
                     LGLEAVKSGLVQETLSGSAYWFPPLPPASIELTSPAYLLSIYDEYISGYKYRSAIGEV
                     EVGEKLFALGNALSYIVVLDGQIVGTWKRTLTRAGVTVHIDLFAQLSKRDLYAITAAQ
                     RYGAFLQLPVTFGQGLSTAKS"
     gene            complement(2080..2259)
                     /locus_tag="VN363_03290"
     CDS             complement(2080..2259)
                     /locus_tag="VN363_03290"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83563.1"
                     /translation="MPPVNIARDRLINQRIAAAPLQTPAEVVDWLVAVQAQDYAGAKW
                     ALGLRMSSAVDMRHR"
     gene            complement(2287..>4997)
                     /locus_tag="VN363_03295"
     CDS             complement(2287..>4997)
                     /locus_tag="VN363_03295"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HWQ83564.1"
                     /translation="PNPFPGASQALLTVERGEVLRHELLSLPPGGLEVALPLLAEDAP
                     NVYASVTLLGRGEGGIDFRFGLLNLDVAPAEFTLNVTLTSSPQQAGPGDEVTFEIQVT
                     DQQGEPVEGEFSISVVDLAVLALADPNAPDILPAYYGQQSLGVRTSMALAAYPGRVLN
                     MPGGLGGGGGDMVAEVVRDNFPDTAYWNASVVTGSDGRAVLSLRLPDSLTTWQIDLRG
                     ITQASQVGQAQAQVVATQDLLVRPVTPQFFVAGDHSLLAAIVQNNTPAELSVNVQLQA
                     TGLALDVTSASLQTVQVPANGRQRVEWWGTVQDVPELDLIFAAEAENGLNDAARPGLG
                     ALPVMHYTAPRTFSTAGVLDSAGERLELVSLPRSFDPAGSGSLRLEVSPSLAAGLIDA
                     LDYLEHYPYECTEQTLSRFLPNLETYRALQISGSSSPAALQAQLERTLNDGLTRLQAT
                     QSQDGGWSWWSGGASDPYVTAYVVFGLSRTRDAGIEINAASLQRASDYLLSTLLPLAS
                     LADTLPDAWQLDRQAFIHFALTQAGVQDLSGVRTLAGYAERLSPWANALLALTLEQLS
                     PGDAVVEQILSSLQASALRSASGVHWELPAGGLQNMHTGLSNSAIVLYALAQLEPTSP
                     LLSDSARYLMVARQAGKGWNATYTTAWSIMALSRYMQTTGELAAGYGFGASLNGVPLL
                     SGQAGGTDNLTPILATVPLMDLYPDYPNALQINRTQGGGRLYYTADLSVSQPVEQVGE
                     LSQGLRLERKYYPAQSDCTIEACPPVGAGRAGEMVRVRLTLTLPQDAYYLLVEDYLPA
                     GAEILNSSLKTSQQGVEPDPAIPLFGGRDLFTDGWGWWYFHNPQIYAERIAWAADYVP
                     AGTYELTYTLVLLQPGEYRVLPARGWQFYFPDVQGSSPGALFRIEP"
BASE COUNT         1034 a         1396 c         1659 g          908 t
ORIGIN      
        1 cagcgggatt atgaaatgtt ctcgggcgga gaggcgtttc gggtgaattt tgccatccgc
       61 ctggccctat cggagatcct ggcgcagcgg gcaggggcac gcctgcaaac cctggtgatc
      121 gatgagggct tcggcagcca ggatgccctg ggccggcagc gcctgatcga agcgatcaat
      181 atggtgcggg aagatttcgc caaaattctg gtgatcactc atattgatga gctcaaggat
      241 gccttcccca accgcattga agtggagaaa acggatcgcg gctcgacggt ccgggtaagc
      301 taatttacag gtgattatgg acaatcaaaa acgagtttta ttggcggtac tggctcaccc
      361 ggatgatgaa tctttcggga tgggcgggac gctggcgttg tatgcccggc agggggtagc
      421 ggtgcatctg gtatgcgcca cccgcggcga agctggcgag gtggatgaag agtatttgga
      481 gggcttcggc tcgatcgccg agcggcggga gagtgagctg cgctgcgcgg cagaaatgtt
      541 gggtctggca gatgtccatt tcctggatta tcgtgattca ggtatgaccg gctcaccggc
      601 aaaccagcac ccacaggcgc tggcagccgc gccgctcctg gaagttgcag aaaaaatagc
      661 ctgctggatc cgggagctgc agccgcaggt ggtggtcaca ttcgacccga tcgggggcta
      721 tcgccacccc gaccatattg ccatccacca ggcaaccgtg aaagctttta caatggcggc
      781 tgatccacaa atctgggccg gaggcctggc accctttcgg acccagaagc tgtactatca
      841 caccatccca cgcgggttgt tccgctgggg ggtacgattg ctgcgcctgt ttgggcgcga
      901 tccacgccgc tgggggcgga atggagatat cgatctggta gctctggcgg cagatgattt
      961 ccctgtgcag gcgcggatcg atatccgtcc ggttgccggg attaaagcgc aagcctctgc
     1021 ctgccatgcc agtcagggcg gtccggtgat gcggcagggc ttattcggat gggttacccg
     1081 cctgggaaac cggtatgaaa attacatgct ggctgaccca ccgccggttc cgggcttgat
     1141 cgaaaacgat ttcttcgcag atatcagctc ttagctgtcg ataatccttg cccgaatgtg
     1201 accggcagct ggaggaatgc cccatagcgc tgggcggcgg taatggcata gagatctctt
     1261 ttgctcagct gggcgaacag gtcgatatgg acggtcacgc cagctctggt aagggtacgc
     1321 ttccaggtgc ccacaatttg cccatccagc accacgatat agctcagggc attccccagt
     1381 gcgaacagct tttctcccac ctccacctcg ccgatggcgg aacggtattt gtatcccgat
     1441 atgtattcat cataaattga cagcaggtaa gccggtgagg taagctcaat cgaggcgggc
     1501 ggcaagggtg gaaaccaata ggcgctgcct gacagggtct cctgcacaag cccggatttg
     1561 actgcctcca ggccaagccg ggcatcggca agggtcagcc cagaccatct atcaaaatcc
     1621 tggactgtgg ctggatcgcg actttgaaag tagcgccggc tcagctccgt aagggctgtc
     1681 tggcggtcaa gagatgcgac tggaggggcg cgctcatcga gcagtgcata ggtgaactgc
     1741 ttgccgcggc gggcgccgct gcagatcaga ccgtccagct cggcagacat caggatatag
     1801 gccaggtgca gcccgctcaa aatggatatg ccggcctgct ggagcgcatc ccgcagctct
     1861 gtgcgggtga gctgcctgcc gctgaggatt tttgccagcg cggtctggct gcgtttaagg
     1921 acggcgcttt ccagacccag cttacgaagc atactggcat tgaccgcgtg gacccgcggt
     1981 gcggtaagaa tcagcagcca gcgaatatcc gccggcgtta caaaatgcca ggtgggccgc
     2041 aggatatggg taggcaaaat ggagccttcg gaaaaggctt tatcgatggc gcatgtccac
     2101 cgcgctggac atgcgcaggc caagcgccca cttcgccccg gcatagtcct gagcctggac
     2161 agcgaccagc caatcgacga cttccgcggg cgtctggaga ggtgctgcgg cgatgcgctg
     2221 gttgatcagg cggtcacgag cgatatttac aggcggcatg gccggcagcc agtctggcga
     2281 tccggatcag ggctcgatgc ggaagagtgc accgggggaa ctgccctgga catcggggaa
     2341 ataaaactgc cagccgcggg cgggcaggac ccggtattcg ccaggctgga gtaagaccag
     2401 ggtgtaagtc agctcgtaag tgccggcagg gacgtaatct gcggcccagg cgatgcgctc
     2461 ggcatatatc tgagggttgt gaaagtacca ccagccccag ccatcggtaa acaggtcgcg
     2521 cccgccaaaa agaggaatgg cagggtcggg ttcaacgcct tgctggctgg ttttcaggct
     2581 ggaattgagg atctcagcgc cagcaggcag gtaatcctcc accagcagat agtaagcgtc
     2641 ttgaggcagg gtgagggtga ggcggacgcg gaccatctct ccggcgcgtc ctgcaccaac
     2701 cggtggacag gcttcaatgg tgcagtcgct ttgggcgggg tagtattttc gctccaggcg
     2761 cagtccctgg ctcagctcac cgacctgctc gaccggctgg ctgacgctca gatcggccgt
     2821 gtaatacagc ctaccgcctc cctgagtgcg gttgatctgc agcgcattgg ggtaatcggg
     2881 atagagatcc atcaggggga ctgtggcaag gataggggtc agattatccg tgccgcccgc
     2941 ctgaccgctg agcagcggaa cgccattcag ggatgcacca aacccgtaac cggcggctaa
     3001 ttcgccagtg gtttgcatgt agcgggataa cgccatgatc gaccaggctg tggtataggt
     3061 ggcgttccag cctttgccag cctggcgggc caccatcaga tagcgggcac tgtcgctcaa
     3121 cagaggagaa gttggttcta actgcgccag ggcgtagagc acgatggcgc tgttggagag
     3181 gccggtatgc atattctgca ggccgccggc aggaagttcc caatggacac cagaagctga
     3241 gcgcagggca gaagcctgca ggctggagag aatttgctct acaacggcgt cacccggaga
     3301 aagctgctcg agggtgaggg ccagcagggc gttggcccag gggctcaagc gctcagcata
     3361 gcctgccagg gtgcgcacgc cactcagatc ctgtacaccg gcctgggtga gtgcaaagtg
     3421 gatgaaagcc tggcgatcca gctgccaggc atccggaagg gtatcggcga gcgaagccag
     3481 cggcagcagg gtggataaca gatagtcgga ggcgcgctgg aggctggcgg cattgatctc
     3541 gatcccggca tcgcgggtgc ggctgagccc gaacacaaca taagcggtaa cgtaagggtc
     3601 gctggcgccg ccgctccacc acgaccagcc gccatcctgc gattgggtgg cctgcaggcg
     3661 ggtgaggcca tcattcaggg tgcgctcgag ctgggcttgc aaggcggcgg gagagctgct
     3721 gccggatatc tgcagggcgc gataggtttc caggttaggg aggaaccggg acagggtctg
     3781 ctcggtacat tcgtagggat aatgctccag atagtcgagg gcgtcgatca gaccagctgc
     3841 cagagaagga gatacttcca ggcgcaggct gccactgcct gccgggtcaa aggagcgcgg
     3901 caggctgacc agctcgaggc gctcaccggc tgaatccaga accccggcgg tactgaaggt
     3961 gcgcggggcg gtatagtgca tgaccggcaa agcacccaga ccggggcggg cggcatcatt
     4021 caagccattt tcagcctcgg ccgcgaaaat cagatccagc tcggggacat cctggacggt
     4081 gccccaccat tccacccgct ggcgaccgtt ggcaggaacc tgaacggtct ggagcgatgc
     4141 gctggttaca tccagagcca gcccagtggc ttgcagctgg acgttgacgg ataattccgc
     4201 gggggtattg ttctggacga tggctgccag gagggaatgg tcaccggcta cgaaaaactg
     4261 aggtgtaacc gggcgaacga gcaggtcctg ggtggcgacg acctgggcct gggcctgccc
     4321 cacctggctg gcctgggtga tgccgcgcaa atcgatttgc caggtggtca ggctgtcggg
     4381 caggcgcagg ctgaggacag ccctgccatc ggagccagtg acgaccgagg cattccaata
     4441 ggcggtatca gggaaattat cgcggaccac ctcggcgacc atatccccac ctccgccgcc
     4501 cagaccaccc ggcatattca ggacgcggcc ggggtaagca gccagggcca tgctggtgcg
     4561 cacgcccagg ctctgctgac cgtagtatgc aggcagaatg tccggggcgt tggggtcagc
     4621 gagagccagg acggccagat cgactacaga gatggagaat tcaccttcta caggctcacc
     4681 ctgctggtcg gtgacctgga tctcaaaggt aacctcatca ccgggtccgg cttgctgggg
     4741 agagctggtg agcgtcacat tcagcgtaaa ttccgccgga gcgacgtcga gattgagcag
     4801 tccaaagcga aaatcaatcc caccttcacc gcggccgagc agcgtgacgc tggcgtagac
     4861 attgggggcg tcttcggcga gcaggggcag cgccacctcc aatccaccgg gcggcaggct
     4921 gagcagctcg tggcgcagaa cttcaccacg ttcaaccgtc aacaaggcct gactggcgcc
     4981 ggggaaagga ttgggga
//
LOCUS       DATMXK010000177         2772 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161286, whole genome shotgun sequence.
ACCESSION   DATMXK010000177 DATMXK010000000
VERSION     DATMXK010000177.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2772)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2772)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2772
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161286"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1271
                     /locus_tag="VN363_03300"
     CDS             <1..1271
                     /locus_tag="VN363_03300"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83565.1"
                     /translation="EKMLYDNALLGRAYLHAYLQSGDLEFRRVSESTLDFMLRELLAV
                     PDDEGLSGGFYSSLDADSEGEEGKFYLWTETEVQTALAALPGSVELALAAYGFTAGGN
                     FEGRTIPRRVMKDAELAERFGLDASAVPARLAEINQAMLAYRDQRVRPATDDKVLTAW
                     NGLALLFLAEAGRYLKRSDYMAAARRTADFLLSELHPANRLLRAWRAGKASHNAYLED
                     YAALILGLLALYQSDPDPYWYNSALDLTAEMVAHYSDPDGSFYDTRDDQAGLILRPRE
                     VQDNATPSGGALAALALLQLSAFTGDLELRDRVARMLSQVEAAAVRYPTAFAFWLQAA
                     YALIHPYQEVALVGDLSSLAAQELLDLLWDTYRPRIVLAASNVPIPENSPPLLQDRPL
                     LNEQPSVYVCENFICQRPVTRPSDLADLLH"
     gene            complement(1289..2065)
                     /locus_tag="VN363_03305"
     CDS             complement(1289..2065)
                     /locus_tag="VN363_03305"
                     /inference="COORDINATES: protein motif:HMM:TIGR00195.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exodeoxyribonuclease III"
                     /protein_id="HWQ83566.1"
                     /translation="MTETKITTWNVNGLRAVLAKGSLTWLENFQPDVLCLQEIKARPE
                     QIDPGALAPLSPYQAYWNPADKPGYSGVATFTRQEPLSVESGWGNEEYDREGRVILTQ
                     FPDFKLLNIYFPNGQRDHGRLQYKLAFYEELLGLCVAAHERDEKIIICGDFNTAHNEI
                     DLRNPKSNVTTSGFMPEERAWLDRYVESRLVDAFRVLYPDKVQYTWWTYISNARARNV
                     GWRLDFFMVSDSLMARVKDVIIHDDVYGSDHCPVTLVLDF"
     gene            complement(2062..>2772)
                     /locus_tag="VN363_03310"
     CDS             complement(2062..>2772)
                     /locus_tag="VN363_03310"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ83567.1"
                     /translation="KIPTTAQPSSGQFAKVFRSLAETDPEVLSIHISSGLSGTIESAR
                     AAIDQATEANIEAVDTLTLSGAERYQVLAAAWAVKAGWTKKAILERLDQIRKHTELIF
                     VLETLEYLARGGRIGRVQGMLGSLLKLKPVIRVEHTDGKYTTEGRARTMVKALSTLID
                     FLETTYGNSEPLWISVMHGQFLEQAENLARMARERLNVVKLEIMRVSPALGVHTGPEV
                     VGIAAVPMRLMEDVVPGA"
BASE COUNT          568 a          822 c          750 g          632 t
ORIGIN      
        1 ttgaaaaaat gctttacgat aatgctttgt tgggacgggc ttacctgcac gcctatttgc
       61 agagtggtga tttggaattc cgtcgggtaa gtgaatcgac cctggatttc atgctccgcg
      121 agctcctcgc cgtacccgat gatgaaggct tatcgggtgg tttttattca agcctggatg
      181 ccgattcgga gggcgaagaa gggaaattct acctttggac agaaactgaa gtccagacag
      241 cattagccgc tcttcctggc agcgtcgagc tggctttggc tgcgtatggc tttaccgccg
      301 gcggtaactt tgaaggccgg acaattcccc gccgggtaat gaaggatgcc gagctggccg
      361 aacgtttcgg tctggacgct tccgcagttc ccgcccgcct ggctgaaatc aatcaggcaa
      421 tgctcgccta ccgcgaccag cgggttcgcc cggcgacgga cgataaagtc ctgactgcct
      481 ggaatggact ggcgctgctg ttcttagcgg aagccgggcg ctacctgaag cgctcagatt
      541 acatggcggc cgcccgccgg acagcggatt tcctgctcag cgagctgcat cctgccaacc
      601 gcctgctgcg tgcctggcgg gcaggcaaag ccagccataa tgcgtattta gaagattacg
      661 cggcgttgat cctgggactg ctcgctttat accagtctga tcctgatcct tattggtaca
      721 actctgccct ggacctgaca gccgagatgg tcgcccatta cagcgatccg gacggcagct
      781 tctacgacac acgcgatgac caggccgggc tgatcctgcg cccgcgtgag gtccaggata
      841 atgccacacc atccggaggc gctctggctg ctctggctct gctccagctt tcagcattta
      901 ctggggatct ggagctgcgt gatcgggttg cccggatgct cagccaggta gaagccgcgg
      961 ctgtccgcta tccaaccgcc ttcgccttct ggctgcaagc tgcctacgcc ttgatccatc
     1021 cctaccagga agtcgccctg gtgggtgatc tgagcagtct cgcagcccag gagctgcttg
     1081 atttactttg ggatacctac cgtccgcgga tcgttctggc tgccagcaat gtacccattc
     1141 cggagaattc cccacctctg ctccaggatc gtcccctgct gaatgaacag cccagcgtgt
     1201 atgtgtgcga gaattttatc tgccagcgtc cggtcacccg accctcagat ctggcagatc
     1261 tgctgcactg accagcgata gataatcctc aaaagtccaa aaccagtgta accgggcagt
     1321 gatctgatcc gtagacatca tcatggatga tcacatcctt aacccgcgcc atcaggctgt
     1381 ccgagaccat gaagaagtcc agccgccagc ccacattgcg ggcgcgggcg ttggagatat
     1441 acgtccacca ggtgtactgt accttatccg ggtacagcac ccggaaggcg tccaccaggc
     1501 ggctctcaac gtaccggtca agccaggcgc gctcttcggg cataaaccca ctggtggtca
     1561 cattggattt agggttacgc aggtcgattt cgttgtgggc tgtgttgaaa tccccacaga
     1621 tgatgatctt ttcgtcccgt tcatgcgcag ccacacacag gcccagtaat tcttcataaa
     1681 aagccagctt atactgcagc cgcccatgat cccgctgccc attgggaaaa taaatattta
     1741 gcagcttgaa atccgggaac tgggtcagga tcacccgccc ctcgcggtcg tattcctcat
     1801 ttccccatcc agattcgacc gataaaggct cctgcctggt aaaggtggcc acaccgctgt
     1861 atcccggctt atctgctgga ttccaatatg cctgatatgg gctcaggggt gccagtgcgc
     1921 caggatcaat ctgttcaggg cgggctttaa tctcctgcag acacaacaca tctggctgaa
     1981 aattttccag ccaggtcagg ctgcccttcg ccagcacggc gcgcagcccg ttgacattcc
     2041 aggtggtgat tttcgtttcg gtcatgcgcc cggaacaaca tcctccatca aacgcattgg
     2101 cacggctgca atccccacaa cttctgggcc ggtatggaca cccaatgccg gtgaaacgcg
     2161 catgatttcc agcttaacca cgttcaggcg ttcgcgagcc atacgggcta aattttccgc
     2221 ctgctcgaga aactgcccgt gcatcaccga gatccacaag ggttcggaat tgccataagt
     2281 cgtttcgaga aaatcaatca gggtcgaaag cgctttgacc atcgtacgcg ctctcccttc
     2341 ggtggtgtac ttgccgtccg tatgctcgac ccggatgacc ggttttagtt tgagcagcga
     2401 ccctaacatc ccctgcacgc gaccgatccg cccgccccgg gccaggtatt ccagggtttc
     2461 gagcacgaaa atgagctcgg tatgttttcg gatctggtcc aatcgctcca ggatggcttt
     2521 tttcgtccaa cccgctttca cggcccaggc tgctgccagc acctggtacc gctcggcgcc
     2581 cgaaagagtc agcgtatcca cggcttcaat attcgcttca gtagcctgat caatcgccgc
     2641 gcgggcggat tcgatcgtgc cggaaaggcc ggatgatatg tgtatcgaga gcacttcggg
     2701 gtcagtttcg gctaaactcc ggaatacttt ggcaaactgg ccggaagatg gctgggctgt
     2761 agttggaatt tt
//
LOCUS       DATMXK010000178         2195 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_105165, whole genome shotgun sequence.
ACCESSION   DATMXK010000178 DATMXK010000000
VERSION     DATMXK010000178.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2195)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2195)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2195
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105165"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..877)
                     /locus_tag="VN363_03315"
     CDS             complement(<1..877)
                     /locus_tag="VN363_03315"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HWQ83568.1"
                     /translation="MPVTIVLGAQWGDEAKGKIADLLSGSAAVVARFNGGDNAGHTVI
                     NSYGTFKLRLLPNGFSSPDTQCVIGPGVAINLQTLSEEIAMLEERGLPLRERLWVSPR
                     SHVVMPYHPLLEGIYENARGSARTGTTRRGIGPVYADKVSYNGIRLADLMDRTVLADK
                     LGVQLAVKNPLLEAFGLPPLDLHTMLDEILAKFNPIRDVVRETFGMLQDTLTAGKQVV
                     LEGAQAALLDTDWGTYPYSTASTTLAGGITAGLGIAPRWIERVIGVSKAYQTRVGSGP
                     FPTELTGGAGEVMLQK"
     gene            complement(957..1913)
                     /locus_tag="VN363_03320"
     CDS             complement(957..1913)
                     /locus_tag="VN363_03320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559279.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWQ83569.1"
                     /translation="MNFLITGAAGFLGSKLANALTREGHQVRGLDDLSTGDPQALFPD
                     VLFTRGDVNDRPKLWTLLQEVDCVYHLAARVAVPESVLYPREYNAVNVGGTVSLMEAM
                     RDVGVRRVVFISSGAVYGDQGEQPLKETAHPNPRSPYAVSKLAAEYYIRTIGDLWGIE
                     TVCLRVFNAYGPGQHLPPSHPPVIPNFLKQAMRGGTLVMHGEGQQTRDYVYVDDVVST
                     MIAASTAPGINHLVINVGSGVETSIRDLVRLVGETTGAKLEVLTNPRTSPGVSRMCAD
                     LSLAREKLGYQPRFTLSQGLRLTLDRDSRFRIEPPTRPINRA"
BASE COUNT          478 a          648 c          598 g          471 t
ORIGIN      
        1 ccttttgcag catcacttcg ccggcgcctc cagttagctc ggtcgggaaa ggtccactgc
       61 ccacccgggt ttgataagcc ttcgagaccc cgatcacccg ctcgatccag cggggggcga
      121 tgcccaatcc ggcggtaatg cctcctgcca gggttgtgga ggctgtgctg tatggataag
      181 taccccaatc tgtatccagc agagctgcct gagcaccttc caggacgact tgcttgccag
      241 cggttaaggt atcctgcagc atgccaaacg tctcacggac cacatctcga atgggattga
      301 atttcgccag gatttcatcc agcatagtat gcaaatccaa aggaggcaat ccaaaggcct
      361 ccagcagtgg attcttgact gccagctgca cacccagttt atccgccaga actgtccggt
      421 ccatcaggtc agccagccgg atgccgttgt agctgacttt atcagcataa accgggccga
      481 tcccacgccg ggtggtaccc gtgcgggccg accccctggc attctcgtag atcccttcca
      541 ggagagggtg atacggcatt accacatggc tgcgcgggga aacccacaaa cgctcacgga
      601 gaggcagtcc tcgctcctcc agcattgcga tctcttcact cagagtttgc aggttgatcg
      661 ccactcccgg cccaatcaca cactgcgtgt cggggctgga aaagccgttg ggaagcaagc
      721 gcagtttaaa cgtgccgtag gaattaataa ccgtatgccc ggcattgtcc ccgccgttga
      781 agcgcgccac gaccgctgcg ctcccggata acaggtcggc aattttccct ttggcttcat
      841 cgccccactg ggcgcctaaa acgattgtga ccggcatact gaatatctcc tgactgagga
      901 taagttttat ccgacaagct taccggattg atatttctga caaagctgag ggaagtttaa
      961 gcgcgattga tcggccgcgt cggcggctca attcgaaagc gtgaatcgcg gtccagggtc
     1021 agccgcaaac cctgtgagag agtaaagcgc ggctggtagc ctaatttttc acgggccagg
     1081 ctcaaatcgg cgcacatgcg tgatactcct ggcgaggtgc gaggattggt cagaacttcc
     1141 agctttgccc cggtggtttc gcccaccagg cgcaccagat cgcggatgct ggtttccacc
     1201 ccgctcccga cattaatcac cagatggttg attccaggtg cagtggaggc ggcgatcatt
     1261 gtgctgacca catcatcgac atagacataa tcacgggttt gctggccctc accatgcatc
     1321 accagcgttc caccccgcat ggcctgtttt agaaaattgg ggatcaccgg tgggtgagat
     1381 ggcggcaggt gctggccagg tccataagca ttgaacaccc gcaggcagac ggtttcgatg
     1441 ccccataaat cgccaatggt gcgaatataa tattctgccg ccagtttgga aactgcatag
     1501 ggcgagcgcg gattcggatg ggcggtctct ttgagcggct gctcgccctg atccccatag
     1561 actgcccccg acgagataaa cactacccgg cggacgccta catcccgcat cgcttccatt
     1621 aagctcaccg tcccgccgac attcacggca ttgtattcac gcgggtataa cacggattcg
     1681 ggtactgcca cccgggctgc caggtgatag acacaatcga cttcttgcag cagtgtccat
     1741 aacttgggcc ggtcgttgac atctccgcgc gtgaaaagca catcgggaaa aagcgcctgc
     1801 gggtcgcccg tggatagatc atccaggcca cgcacctggt gaccttccct ggtaagagca
     1861 ttggccagtt tggaacctaa aaagccggcg gcgccggtaa taagaaaatt catttccgcc
     1921 ccagtaattg ggagatgata atgggcatca gcctgatttt aacaccaatg gagccgccgg
     1981 tatagcgacc acagcttgct taacaatccg ccttactgag cagccacggc atgtccttca
     2041 tacccgggct gatgatacaa tcccgtatat gtctcttctc aaagggattt acgcgattct
     2101 acgccaggct ctggagctgc tgatcctggc tgctctgctg ggcgggagtg gacagctgcc
     2161 cgtggacctg gttggcaggg tgcgcgctta taccc
//
LOCUS       DATMXK010000179         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_151597, whole genome shotgun sequence.
ACCESSION   DATMXK010000179 DATMXK010000000
VERSION     DATMXK010000179.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151597"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..482)
                     /locus_tag="VN363_03325"
     CDS             complement(<1..482)
                     /locus_tag="VN363_03325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83570.1"
                     /translation="MNTYRDLQLQEIAELDLKLLARALKALRVLTYAADSDIYTDAPE
                     CQASVDQAQEAVQAAYIVLGEIRKAVEPPPIEEPVEVLAHRILDEADEAFAARKVAEE
                     RAAKGRFPRPVSIPDYLFWIEDELRLQGIYRLNQPQEQTQQRIYLQALFELTRELRWL
                     G"
     gene            complement(510..950)
                     /locus_tag="VN363_03330"
     CDS             complement(510..950)
                     /locus_tag="VN363_03330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83571.1"
                     /translation="MDARNNPTHITTGQIQYLPRHRCPKCGAAPRIMSTGGWFQPYQD
                     WFNTALGSWQAYLGYVRFQCNCQHHPEFRGVLRVVRLGDAILPVHFAGFAAAREIGLE
                     PVQINAQRTRQALAFLAAHADPAYTQGTDWPAQRLSALAEIPPV"
     gene            complement(950..2077)
                     /gene="radC"
                     /locus_tag="VN363_03335"
     CDS             complement(950..2077)
                     /gene="radC"
                     /locus_tag="VN363_03335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWQ83572.1"
                     /translation="MNELFQIQSPYPLPRLKRLPLREQPAYRVAQDPDSCNLIELLAA
                     LVGGPQQIEIAEELLASFGRVRKLHQAQIIEIAGIHGLGRQTAIRIKAALALGKRAIL
                     ETEDERPTIHSPADAAALVQYEMSLLEQEYLKVLLLDTRNRVLHNSEIYHGSLNSSQV
                     RVAELFKPAIQRMAAALIVLHNHPSGDPTPSPDDVIVTRAIVQAGKLLDIECLDHIII
                     GHSGRYVSLKEGGLGFDSRIAESPLVYRVREMEVIYTYTRGQALADGVLVDVSEMARE
                     VGFRHPTVITADLHAALTPNARERSYGQSYEGRLWDVLFLAVVAARRAIWASQTAFNV
                     RLAVCPGNRGRLRRQSLHLWLVIGAGDNFEPVITIGFPGDF"
     gene            complement(2193..2726)
                     /locus_tag="VN363_03340"
     CDS             complement(2193..2726)
                     /locus_tag="VN363_03340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83573.1"
                     /translation="MMVLATRRKAAPPPLDLRQNAAVSVDGYFYHVDFGPGYQGPLHR
                     VGKDRRCRCPDGGRCIAVAAVADYLKAGGQRAPDPPPGYYPVAPSVCPICQAEAFFDP
                     HLGSKQRGAGWRCSKAGSLHYWEAHVRVLRQKVKANPWLFPPVVIRQGVWLLAYVGVE
                     TGDQVLYPGILREEVGT"
     gene            complement(2787..3197)
                     /locus_tag="VN363_03345"
     CDS             complement(2787..3197)
                     /locus_tag="VN363_03345"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWQ83574.1"
                     /translation="MSYQELTLIGHLGRDPEMSFTPSGQAVTKFPVAVNRRVRNAMGE
                     QVRETAWFQITAWERQAENANLFLQKGSQVMVKGRLTPDPETGSPRLWERRDGALAAS
                     YDVTAQEIIYLSRREGTPTGESTHAEGIEMEIPF"
     gene            complement(3179..4018)
                     /locus_tag="VN363_03350"
     CDS             complement(3179..4018)
                     /locus_tag="VN363_03350"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HWQ83575.1"
                     /translation="MNPTTEIIPRLSRPLDRKRVRLHSAPGQGAVPFLEDLDVIHTAN
                     QTFDYRWSFDLLSEPHLVLWEQAVTTWDSRRRQRTPVLDPETGQQKMQRTGTVYLTGK
                     VTIELDGMPYTHADVGRLSFTGDTSEALELALSGAASDCLKRCLRQLGSQFGLDLMAK
                     ESVRSAGQSAVVNNGHQAGSHRPTIEGVKPVTGTEQNGLSMTRPWRRFVLSDQAKTRT
                     MRACRWGRCSASRRASVCWLTWLVSSSRPIVTRFANRLKSPRSCCWPRLNPKEAERCP
                     IKN"
BASE COUNT          855 a         1258 c         1257 g          859 t
ORIGIN      
        1 cccagccagc gtaactcgcg agtcaactca aacaacgctt gcaggtagat gcgctgttgg
       61 gtttgctcct gcggttgatt caaccggtag attccttgca aacggagttc atcctcaatc
      121 cagaacaggt aatcggggat cgacaccggc ctgggaaatc tgccttttgc cgcccgctct
      181 tcagccacct tgcgagcagc aaatgcctcg tccgcctcat cgaggatgcg atgggccagg
      241 acttcgacgg gttcttcgat gggaggaggc tcaaccgcct tgcggatttc acccaacact
      301 atataagccg cttgaacggc ctcctgcgcc tggtccacac tggcctggca ctcaggcgca
      361 tcggtgtaga tgtcgctatc ggctgcgtag gtcagcactc gtagagcttt tagtgctctt
      421 gccaacagtt tcaagtccag ctcagcaatt tcctgcagct ggagatctcg ataagtgttc
      481 atcttgcaac ctttcttcaa cgagtaaggt tagacaggtg ggatttccgc caatgcggat
      541 aatctttgag ccggccagtc tgtcccctgg gtgtaggccg ggtcagcgtg cgctgccagg
      601 aaagccagcg cctggcgagt tcgctgggca ttgatttgaa ccggctccag tccgatttct
      661 cgggcagcag caaagccggc gaagtgcacc ggcaggatgg catcccccaa ccggaccaca
      721 cgcaagacgc cgcgaaactc ggggtggtgc tggcagttac actgaaaacg gacatagccc
      781 aggtaagcct gccagctgcc cagagcagtg ttaaaccaat cctggtaggg ttgaaaccat
      841 ccaccagtgg acatgatcct cggcgccgcg ccacacttcg ggcagcgatg ccggggcaga
      901 tactggatct gccctgtagt gatgtgagtg ggattgtttc gggcgtccat caaaaatccc
      961 ccggaaaccc gatggtgatt accggctcaa agttgtctcc cgcgccgatc accagccaca
     1021 ggtgcaggga ttgccggcgc aggcgaccac gattacccgg gcaaaccgcc agacgaacat
     1081 tgaatgccgt ctggcttgcc cagatcgccc tccgggcagc caccacagcc aggaaaagca
     1141 catcccacag tcgcccctca taggattggc cataggagcg ctcgcgagca ttaggtgtca
     1201 gagcggcatg cagatcagcg gtaatgacgg tagggtgacg aaaacccacc tcacgcgcca
     1261 tctcgctgac atccaccagc acgccatctg ccagagcctg ccccctggtg taggtgtaga
     1321 ttacctccat ctcacgcact cggtaaacta gcggggattc tgcaatccgg ctatcgaacc
     1381 cgagcccgcc ctccttcagg gagacgtagc gcccagaatg accgatgatg atatggtcga
     1441 ggcactcgat gtccaggagc ttgccggcct gtacgatggc acgcgttact atcacatcat
     1501 ctgggctggg tgtcgggtcg cccgacgggt ggttgtgaag cacaatcaac gcggcagcca
     1561 tgcgctggat ggcaggcttg aacagctccg ccacccgcac ctgagaagaa ttcagcgagc
     1621 cgtgatagat ctcgctgttg tgcaggacac gattgcgcgt gtccagcagc agcaccttca
     1681 gatattcctg ctccagcagg ctcatttcgt actgcaccag cgcggcagca tccgccgggc
     1741 tgtggatggt ggggcgctca tcctcggttt ccaggatggc acgcttgcca agagccaggg
     1801 ctgccttgat gcggatggca gtctgcctgc ccaggccgtg gataccggcg atctcgatga
     1861 tctgggcctg gtggagtttg cgtacgcgac caaatgacgc cagcagctcc tcggcgatct
     1921 cgatctgctg cgggccgccc accagggcag ctaacagctc gatcagattg caggagtcgg
     1981 ggtcttgcgc cacccggtaa gccggctgct cgcgcagcgg caggcgcttg agccggggta
     2041 gcggataggg ggattggatc tggaaaagct cgttcatggt ggtccgtcct ttccgtattc
     2101 tttaagatgg aggcagggga cggacgtatc cccatgcagg ggaaggacga tccgcccctg
     2161 ccggggtaga gaaacacttc aattcaaaag gctcaggtgc ccacctcctc tcgtaaaatc
     2221 cctgggtaca gtacctggtc gccagtctcg acgccaacgt aagctagcag ccacacaccc
     2281 tggcggatca caaccggtgg gaacagccag ggattggctt tcaccttctg gcgcaggacc
     2341 cgcacatggg cttcccaata gtgcaggctg ccagccttgc tgcaacgcca gcctgcgccg
     2401 cgctgcttgc ttcccagatg agggtcgaag aaggcctccg cctggcagat cgggcagacg
     2461 cttggcgcta cagggtagta accgggcgga ggatcaggcg cccgctggcc gccggctttg
     2521 agatagtcag cgacggctgc aacggcgata caccttccac catctgggca gcggcaacgc
     2581 cggtccttgc ctacccggtg gagcggccct tgatagccag gcccaaaatc aacgtgatag
     2641 aaatagccat ccacactgac cgcggcgttc tggcgcagat cgagaggtgg gggcgcggct
     2701 ttacgccggg tcgccaaaac catcattggc ctcctttctc cgtacggatg attcagttgt
     2761 cagttaaggg gaaatttgat gcgggatcag aagggaattt ccatctcgat cccctcagca
     2821 tgggttgact cgcctgtcgg cgtcccttca cgccggctca ggtatataat ctcctgggcg
     2881 gtgacatcat aagacgccgc cagagcgcca tcacgccgct cccacaaacg cgggctgccg
     2941 gtctcaggat cgggggtgag ccgccccttg accatcacct ggctgccttt ctgcaagaac
     3001 aggttggcgt tttcagcctg gcgctcccag gcagtgatct ggaaccaggc agtctcgcgg
     3061 acctgttcac ccattgcatt gcgtacccgc cggttgactg ccacaggaaa tttagtcact
     3121 gcctggccgc tcggagtaaa gctcatctcc ggatctctcc ccaggtgacc aatgagggtc
     3181 agttcttgat aggacatcgc tcagcctcct tggggttgag tcgcggccag cagcagctgc
     3241 gcggactttt gagcctgttg gcgaagcggg tcacgattgg tcttgaactg ctcaccagcc
     3301 aggtaagcca gcatacgctc gccctccggc tggcggagca cctgccccag cggcatgccc
     3361 gcatagtgcg ggttttggct tgatccgata ggacaaagcg tctccatggc ctggtcatac
     3421 tgagaccatt ttgctccgtg cccgtgacgg gtttcacgcc ctcgatagtc gggcggtgag
     3481 agccggcttg atgcccattg ttgaccacag cagactgacc agcacttcgg acgctctctt
     3541 tagccatgag atcgagcccg aactggctgc ccaactggcg caagcagcgt ttcaggcagt
     3601 cgctggctgc cccgctcaaa gccaactcca gcgcttcgga cgtatcgccg gtaaagctca
     3661 agcgccctac atcagcatgg gtgtatggca tgccatccag ttcgatggta accttgccag
     3721 tcagataaac tgtcccggtg cgctgcattt tttgctggcc ggtttctgga tccagcacgg
     3781 gagtgcgctg gcgacgccgg ctatcccagg tggtgacggc ttgctcccag agcaccaggt
     3841 gcggctcaga gagcaggtcg aacgaccagc gatagtcgaa ggtctggttg gcggtatgga
     3901 tcacgtctaa atcttccaga aagggaactg cgccctgacc tggtgctgaa tgcagcctga
     3961 cgcgtttgcg gtccagcggc cgtgacaggc gcgggatgat ttctgtcgta ggattcatag
     4021 gacctccttg ggttggattg cgagacatgt gaatgaaacg aacgagcagg tgaactgtca
     4081 atagcctcct ttcaatcagg tatggaatgc tgtatacgtg caaggcccgg cgcggagccg
     4141 gtttgggggg ttggatcgcc agaagctgtt caggcgacag atgattgggc tcgaatgata
     4201 tttaagtcgg atcatctgtc tcctcgaat
//
LOCUS       DATMXK010000180         4577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_105947, whole genome shotgun sequence.
ACCESSION   DATMXK010000180 DATMXK010000000
VERSION     DATMXK010000180.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4577
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105947"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..950
                     /locus_tag="VN363_03355"
     CDS             <1..950
                     /locus_tag="VN363_03355"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWQ83576.1"
                     /translation="PMGKDPRGRSYQEHKFDRDQPEIHERLREIRQIFDAYPGDRVAI
                     GETYFDDPRELMAYSGKDQDELHLPFNFSLINLPWEAGKFRREIGNYYAALLPGAAPN
                     FVLGNHDRHRLASRYGQENHRSAALLLLTLKGSPTLYYGDELGMIDGEIPPERYQDPV
                     MTRKPDTDEGRDPERTPMQWDSSPNAGFSPAGVDTWLPVNPDFQKINVQDELKDPDST
                     LNYYRRLLAMRKSEPALQEGEIDLLGSMPDEVLAFTRIADTQTLLVAINFGYQIHRVE
                     LPEQVQGGAAIFSTLPGETKLDRTNLWLRPHEGVLIARR"
     gene            complement(933..2513)
                     /locus_tag="VN363_03360"
     CDS             complement(933..2513)
                     /locus_tag="VN363_03360"
                     /EC_number="6.3.2.13"
                     /inference="COORDINATES: protein motif:HMM:NF001126.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016874 - ligase activity [Evidence IEA];
                     GO_process: GO:0008360 - regulation of cell shape
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA];
                     GO_process: GO:0051301 - cell division [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanyl-D-glutamate--2,
                     6-diaminopimelate ligase"
                     /protein_id="HWQ83577.1"
                     /translation="MDPVPGVPLSTLLNALPWPVQRPIPDPLIRGIVLDSRQVRPGDL
                     FMVLVGGNVDAHRYIPDAVQRGAAAVVGSRSAENLDVPYIQIAKDRARSTLAKLSAAL
                     YGNPARQMVMIGVTGTDGKTTTSNLIYQILRAAGISVGLISTVNAIIGDETIDTGFHV
                     TTPEAPEVQRYLARMHAAGITHVILEATSHGLAQERVAECDFDLGVVTNITHEHLDYH
                     GSYEAYRAAKARLFTSLSRTPAKAISPPRAAILNRDDQSYAFLSGVTQVAQISYGFDI
                     QSDWQPFEISMSLNSSHFVAKGKDHIGQPVRLPLAMSLVGAYNVSNALAAVACCVQGL
                     GIQLHAVRQGIASLPGVPGRMERIDLGQPFTAIVDFAHTPNALTRTLQAARQLTSGRV
                     IAIFGSAGLRDRQKRRMMAEVSAELADISILTAEDPRTESLSSILEEMAQGALSKGGV
                     EGQSYWRVHDRGEAMRLAVELAQPGDLVIACGKGHEQSMCFGEVEYPWDDRVALQAAL
                     SELLGVPGPAMPFLPPGD"
     gene            complement(2642..3376)
                     /gene="atpF"
                     /locus_tag="VN363_03365"
     CDS             complement(2642..3376)
                     /gene="atpF"
                     /locus_tag="VN363_03365"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01144.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit B"
                     /protein_id="HWQ83578.1"
                     /translation="MEALGISLGYLIVQILNFLIVLVVLNAWVFKPIMGVFQKRREKI
                     SQGLEDARVAAEARANAEKEAREIIAKAETERQQKVRDASQQAEQTAKDVLAQADVQA
                     AKIKDNAQAEIAQERNQILADVRGQVAALAMSATQKLLGEALDEKRQRALIDEFFSGV
                     KAGKVTILESGMAGDAVEVTSALPLTESERQAVETDVLSKIGTQAAVTFRIDPGILGG
                     LVVKVGDKILDGSVAGKLDTMRQSLH"
     gene            complement(3407..3661)
                     /gene="atpE"
                     /locus_tag="VN363_03370"
     CDS             complement(3407..3661)
                     /gene="atpE"
                     /locus_tag="VN363_03370"
                     /EC_number="7.1.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000276 - mitochondrial
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_component: GO:0045263 - proton-transporting ATP
                     synthase complex, coupling factor F(o) [Evidence IEA];
                     GO_component: GO:0045264 - plasma membrane
                     proton-transporting ATP synthase complex, coupling factor
                     F(o) [Evidence IEA];
                     GO_function: GO:0046933 - proton-transporting ATP synthase
                     activity, rotational mechanism [Evidence IEA];
                     GO_process: GO:0015986 - proton motive force-driven ATP
                     synthesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP synthase F0 subunit C"
                     /protein_id="HWQ83579.1"
                     /translation="MELTPEVAEIIIRGLKYIGAGLAMVGTLGAGLGIGILGNGALQG
                     IARNPDAAGTIQINMILAIAFAEAVAIYALVVALVILFVG"
     gene            complement(3752..>4577)
                     /locus_tag="VN363_03375"
     CDS             complement(3752..>4577)
                     /locus_tag="VN363_03375"
                     /inference="COORDINATES: protein motif:HMM:NF012347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="F0F1 ATP synthase subunit A"
                     /protein_id="HWQ83580.1"
                     /translation="LIDLVLILLAFGVKRNLGDENTPSRGVANVFEMIVEGLYNLVES
                     TAGSKWAKQFFPWVATIILLVLGSNLLKLFPGFESIGIVHEAHEGGYPIQQVIPGIVN
                     LLAVEEGAEPAGEHLYELVPFFRSPSTDLNFTVALALTTIVVVQIFGVKANGLGYFTK
                     FFNFGPFLKIWYTKKLGVFDVIMPFIDIIVGLLELIAEMAKIVSFSFRLFGALFGGAI
                     LVAVLGTLVPAVQFGLLFLELFIGSIQALVFGMLALVFMTVATQSHGHGGEEGEAH"
BASE COUNT         1140 a         1267 c         1190 g          980 t
ORIGIN      
        1 atcctatggg caaggatccc agggggcgca gctaccagga gcataaattc gaccgtgacc
       61 agccagaaat ccacgaacgc ctgcgagaaa tccgccagat attcgatgcc tatcccggcg
      121 accgggttgc gatcggtgag acctatttcg atgacccccg cgagctgatg gcgtattcag
      181 gtaaggatca ggatgagctg cacctgccat tcaatttctc gctgatcaat ctgccgtggg
      241 aggcgggaaa attccgccgt gaaatcggta attattacgc tgccttactc ccaggagctg
      301 caccgaattt tgttttagga aaccatgacc ggcaccggct ggcaagccgg tatggacagg
      361 aaaaccatcg ctcggccgcc ctgcttctgc tcaccctgaa aggctctccg accctgtatt
      421 atggagatga gctgggtatg atcgacgggg agataccccc agagcgctat caggatccgg
      481 tgatgacacg caaaccggat accgatgaag gccgcgatcc cgagcgcacg ccaatgcagt
      541 gggacagctc acccaatgca ggcttctcgc cagcaggtgt ggacacctgg ctgcccgtga
      601 atccggattt ccagaagata aatgtgcagg atgagctgaa ggatcccgat tccacattaa
      661 attactaccg gcggctgctg gcaatgcgaa aatctgagcc ggcccttcag gagggtgaga
      721 ttgatctgct tgggagtatg cctgatgagg tgctcgcgtt tacccgaata gcggataccc
      781 agacccttct ggtggcaatc aatttcgggt atcaaataca ccgggttgag ctgcccgagc
      841 aagttcaggg tggagcagcc attttctcga ccctgccagg cgaaaccaaa ttggacagaa
      901 ccaatctgtg gctgcgcccg catgaaggtg tgctaatcgc caggcggtaa aaacggcatg
      961 gcgggtccag ggacgcccag gagctctgat aaggcagcct gcagcgcaac ccggtcatcc
     1021 cagggatatt caacctcacc aaagcacatc gactgctcat gccccttacc gcaggcaatc
     1081 actaaatcac ctggctgagc cagctctaca gccaggcgca tagcctcgcc gcgatcgtgt
     1141 actcgccagt aactttgacc ctccactccg cccttgctga gtgctccttg agccatctct
     1201 tccagaatgc tggaaagtga ttcagtgcgc gggtcttcgg ctgtcaggat ggagatgtca
     1261 gccagctcag cggaaacttc cgccatcatc cggcgcttct ggcggtcgcg cagtccagcc
     1321 gagccgaaga tggcaatcac cctgccagag gtaagctggc gagccgcctg taaggttcta
     1381 gtgagagcgt tgggggtatg ggcaaaatcg acgatagccg taaacggctg gcccaggtca
     1441 atcctctcca tacgacccgg cactccgggt aaactggcaa taccctggcg gactgcatgc
     1501 aattgaattc ccaatccctg gacacaacag gcgactgcag ccagggcatt ggatacgttg
     1561 taagccccta ccaggctcat cgctagcggc agccggactg gctgtcctat gtgatccttc
     1621 cctttagcaa caaaatgact gctattaaga gacatactga tctcgaatgg ctgccaatcc
     1681 gactggatat caaaaccata gctgatctgg gcaacctgag tcacacccga cagaaaggca
     1741 tacgattggt cgtctcgatt taatatggca gccctgggcg gagaaattgc cttggcgggt
     1801 gtcctgctga gcgacgtaaa caggcgggcc ttggctgccc ggtaagcctc ataagaacca
     1861 tggtaatcga gatgctcgtg ggtaatgttg gtaaccacac cgagatcgaa atcacattcg
     1921 gctacacgct cctgagccaa gccgtgcgag gttgcctcga ggatcacatg ggttatcccg
     1981 gcggcatgca tccgggcaag gtaacgctgc acttccgggg cttcaggggt ggtcacatga
     2041 aaaccagtat cgattgtctc atccccgata atcgcgttca cagtggagat caaccctaca
     2101 gagatgccgg cagcccgcaa tatctgatag atcaggttgc tggtggtcgt cttcccatct
     2161 gtcccggtaa ccccgatcat gaccatctgg cgagcagggt tgccatacag ggcggcagac
     2221 agttttgcca gggttgaacg cgccctgtcc tttgcgatct gaatataggg aacatctaaa
     2281 ttctctgcag agcgagagcc aacgactgca gctgcgccgc gctggactgc atcggggata
     2341 tagcggtggg catcgacatt cccacccacc aggaccataa acaggtcgcc aggacgcacc
     2401 tgacgtgaat cgaggacaat cccgcggatc agaggatcag ggattggtcg ttggacaggc
     2461 catggcaagg catttagcaa tgtggataac ggtacaccgg gtactggatc catgggcata
     2521 tcctacaaga tgtgaaatgg cgctcaaggg aaatcttgag cgccatctga ttaccaaatc
     2581 cggttaaaac cgaaacagaa caattaaatt tctaaggcct gacgctaaat cagggtcgat
     2641 ctcagtgcag gctctggcgc attgtatcga gcttgccagc caccgagcca tcgaggattt
     2701 tatcaccgac ttttaccacc agtccgccca gaatccccgg gtcgatccgg aaggtaactg
     2761 ccgcctgagt gccgatctta gataggacat cagtttcaac tgcctggcgt tcgctttcgg
     2821 taaggggcaa agcgctggtg acttcgacag catcgccagc catccccgat tccaggatag
     2881 taacttttcc agccttgaca cccgagaaaa attcatcgat cagagctcgc tggcgctttt
     2941 cgtccagtgc ttcgccgagt aatttttggg tggcagacat cgccagagca gctacctgac
     3001 cgcgcacatc cgccagaatc tggttgcgct cctgggcaat ttctgcctga gcgttatcct
     3061 tgattttcgc ggcctggaca tcggcctggg cgagcacatc tttggcagtt tgctcagcct
     3121 gttggctcgc atcccgcacc ttctgctgtc tttctgtttc agctttggcg ataatctcgc
     3181 gggcttcctt ttcagcattg gcgcgtgctt cggcagctac acgagcatct tccaaaccct
     3241 gggagatttt ttcccggcgc ttctggaaaa cacccatgat cggtttaaat acccacgcat
     3301 tcagaactac caggacgatc aggaagttca ggatctgaac gattaaatac cctagactta
     3361 tacctaatgc ttccaacttg tcctccttat caggtcttct gctgggctag ccgacgaaaa
     3421 ggatgaccag agctaccacg agggcgtaaa tagccaccgc ttcagcaaag gcgattgcca
     3481 ggatcatgtt gatctggatt gtgccagctg catccggatt gcgggcgata ccttgcagag
     3541 cgccattgcc aaggatacca attcccaggc cggcgccgag tgtacctacc atggccagac
     3601 cagcgccaat atacttaagc cctctaataa tgatctcggc aacttcaggt gtcaattcca
     3661 ttttgaaaaa cctcctccaa gtgtgagttt ggattattga tgatgataaa tcagtaaacg
     3721 gtcgaaaccg tgaaaacccg gctgggaaag tttagtgtgc ttcgccttct tcaccgccat
     3781 gcccatgaga ttgtgtggca acggtcataa agaccaacgc aagcatccca aaaaccagcg
     3841 cctggatcga tccgataaac agctccagga ataacagccc aaactggacc gctggaacca
     3901 gggttcctaa gactgcaacc aggatcgctc caccaaacag ggcgccaaaa agacggaagg
     3961 agaacgaaac gattttggcc atctcagcga ttaattccag cagaccaaca atgatgtcaa
     4021 tgaacggcat gatgacatca aaaacgccca gcttcttggt gtaccaaatt ttcaggaagg
     4081 gaccaaaatt aaagaatttt gtgaagtatc ccaagccatt ggctttgaca ccaaagatct
     4141 gcaccacaac aatcgttgtc agggcaagtg ctacggtgaa attcaagtca gtggatgggc
     4201 tgcggaaaaa tggcaccagc tcatacaggt gttcaccggc tggctcagcg ccctcttcaa
     4261 ccgctaacaa attaacaatc ccagggatta cctgttggat cggatagccg ccttcatgcg
     4321 cttcgtggac gatccctatg gattcaaagc cggggaaaag tttaagtaaa ttagatccga
     4381 gtaccagcag gatgatcgtt gctacccagg gaaagaattg tttggcccat ttagagccag
     4441 cagttgattc caccagatta tagaggcctt ccacgatcat ctcaaagaca ttcgccacac
     4501 cgcgggaagg cgtgttctca tcacccaggt tgcgcttaac tccgaatgcc agcaaaatca
     4561 aaaccagatc gataagc
//
LOCUS       DATMXK010000181         7301 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_96995, whole genome shotgun sequence.
ACCESSION   DATMXK010000181 DATMXK010000000
VERSION     DATMXK010000181.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7301)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7301)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7301
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96995"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(216..305)
                     /locus_tag="VN363_03380"
     tRNA            complement(216..305)
                     /locus_tag="VN363_03380"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(269..271),aa:Ser,seq:gct)
     gene            complement(425..509)
                     /locus_tag="VN363_03385"
     tRNA            complement(425..509)
                     /locus_tag="VN363_03385"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(473..475),aa:Ser,seq:tga)
     gene            619..2139
                     /gene="murJ"
                     /locus_tag="VN363_03390"
     CDS             619..2139
                     /gene="murJ"
                     /locus_tag="VN363_03390"
                     /inference="COORDINATES: protein motif:HMM:TIGR01695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0034204 - lipid translocation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="murein biosynthesis integral membrane protein
                     MurJ"
                     /protein_id="HWQ83581.1"
                     /translation="MSRLVRSTLIVVIFFGLEKGLGFIRQVAIAHQFGLSPELDAFNA
                     ANNIPDLIFALISGGALAMALIPVLSETIQKHSRSDAWELFSRVANLVFLVTAGLSVI
                     VALLAEALVGWRLGIAPGFSTPQQELVADLMRLNLFATMLLSLGGLVIAGLQSNQHFL
                     LPALAPSMYDLGMLFGVFILSPEQGYQLGSLTLPAFGMGIHGLVYGTILGSALFLLVQ
                     IPGLLRFGFRWKPILGLFNPAVQQVLSLLGPRVAHMFFIQVTFLGQDNIASRLNAGSI
                     SALVYGWLFMQVPETLIGTALGTVLLPTISEQFARQDLDKFQTTLNHIIRLILALTIP
                     SAVLVGMVLPPVVAILGLDAAGTNLVVWTARAYLLGLMGHSLLEVAARAFYARQAPRT
                     PMLSAALNAAVFLSLAAVLASPLGAPGIALANSIAFTSQALLLLFILRKSFPGLLKVG
                     GTLVRVVLISAVAGAVVFGLLQLSLPALPLAALALLAGTVIVIPFILPEIKLLLRL"
     gene            2136..3326
                     /locus_tag="VN363_03395"
     CDS             2136..3326
                     /locus_tag="VN363_03395"
                     /inference="COORDINATES: protein motif:HMM:TIGR04350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PatB family C-S lyase"
                     /protein_id="HWQ83582.1"
                     /translation="MTTYNFDHTPDRRGLDCAKWGYYAPDVIPLWVADMDFRPPQPVL
                     DALHDQIDTVGLGYPLADGNDDLTRLRALLVERMQHLYHWQIKPQDIVFVPGVVTAFH
                     MASYACVEPNGAILVQPPVYPPIWHAARTTGVLAQEAPLVQLPGGNYAVDWECFEGAI
                     TPETRLFLLCNPHNPVGRVFTRPELEKFAEICLRHNVLICSDEIHADLIYSGHSHIPI
                     ASLAPEIAERTITLMAPSKTFNMAGLQCSFAIIPNAELRKQFQRSNKGLASWVNTLGL
                     VAAQAAYAEGQEWLDQVLVYLQGNRDFLARFVQERLPGIHMVKAEGTYLAWLDCRELA
                     LPGGPYAFFLEKARVALNDGQTFGPGGEGFVRLNFACTRATLEQALLQIQQAVAGTFD
                     QAAS"
     gene            3344..5236
                     /locus_tag="VN363_03400"
     CDS             3344..5236
                     /locus_tag="VN363_03400"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWQ83583.1"
                     /translation="MRIYPILSILITLLAFLTANVYPVRIPSEPASPEWQSRVDPWVL
                     ENAASGETEFLISLNEQADLSGALGLKTQAAKGRLVVETLVETARRTQAPILRRLEEA
                     GATYKAYWISNLIWVRGEAGLIQELASRPDVAHLYANPWVELDILPDKPVDEIARPAA
                     INTIEWNIARVNADQVWAAGFTGQGVVIGGQDTGYEWDHPALKNQYRGWDSSTQTANH
                     AYSWHDAIQSGSGNECGFDSPFPCDDRGHGTHTMGIMVGDDGSGNQIGMAPGARWIGC
                     RNMENGLGSPQTYLECYQWFVAPTDLNNTNPRPELAPQVINNSWSCPPEEGCTDPLVL
                     QQVVQNVHAAGILTVHSAGNSGPTCSTVNTPAAIYAESFTVGNTTPDDTISPSSSRGP
                     VTVDGSERLKPDISAPGTNIRSSYAGGGYTQHSGTSMAGPHVAGLGALLISANPLLRG
                     QVDELETIIQQSAVPLTPTTACGEDIAGVSVPNNTYGWGRIDALHAYEYYQLEVSATS
                     SASVISPGEVITYTLSAANSGEAGLSSITLINELSAGITFQNASNGGVLDGNQIIWAL
                     GNLAVGEIRQVQFSVSTPTDRIGPIINANYYASSDQVISAHGPPLSVWAAHGILYQPL
                     IPYLAP"
     gene            complement(5295..6857)
                     /locus_tag="VN363_03405"
     CDS             complement(5295..6857)
                     /locus_tag="VN363_03405"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558901.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83584.1"
                     /translation="MDDGLALEVRNITKRFPGVLANNRVNFNLYKGEIHALLGENGAG
                     KSTLMNIIYGLYSPDEGEIYMDGQGEGEAKKVEIRTPHDAIQHGIGMVHQHFMLVPVF
                     TVTENIILGDEITRGVALDLNTSRDNIRRVSQEYGLDVDPDAIVEELPVGIQQRVEIV
                     KALYRNAKILVLDEPTAVLTPQEVEDLFRIIHQLTERGVSVIFITHKLKEVLAVADRI
                     TVMRRGEVVGTTTPQETTEQRLAAMMVGREVLLRVEKGPAKPGKVVLDVKNLNVNDDQ
                     GHPVVRDVSFQVFAGEILGIAGVQGNGQTELAEGLTGLRSLKSGSIELNGEQVPAIDP
                     RTLVETGMAHVPEDRHKHGLVLQHSIADNLVLNTYYHEPFSRGLILDQDAVKENAERL
                     VKEFDVRTPSYTLPASTLSGGNQQKVIVAREFSRPIHMLIANQPTRGLDVGSIEYIHR
                     QIVAKRDQGIAVLLISAELDEITSLSDRIAVMYHGQIVDVVDAEAVSREELGLMMAGG
                     QVEKTVPESLAG"
     gene            complement(6870..>7301)
                     /locus_tag="VN363_03410"
     CDS             complement(6870..>7301)
                     /locus_tag="VN363_03410"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83585.1"
                     /translation="RTAWGLRTRAVGEHPRAADTVGINVYKIQYVNTIIAGMMAGLAG
                     AFLTLEAVGSFERGMTNGRGFVALAVMIFGKWTPFGAWGAALLFGFTSALQTQLQFGG
                     QIAIPHQFIGMLPYLMTIIVLAGFVGRSRAPAAEGEPYTKE"
BASE COUNT         1507 a         2241 c         1864 g         1689 t
ORIGIN      
        1 ctcctcgcca atcactgccg aaattttcgc ggttgaatca gcccgggtgg cataagtaat
       61 taatattcgt tcgttcattt ttgctttcct acttatttag attaccccaa tgaacctacg
      121 taacaccttc atcgcttaca ggttcagcct gaattttgtt ggataaataa aagagagcaa
      181 ccggggtttt cgttgctctc aggttctaaa ggcagcgggg agggagggat tcgaaccctc
      241 ggccgagttt aacgcccggc actcacttag caggcgagcc cattaagcca ctctggcacc
      301 tccccaggcg caagcgattg taccataaca atccagtgga tacaatcaaa gaggcgaaca
      361 ctaaatcctt caggggtcag cagctgcaat catctgccag taccctgaat ttcaagtttt
      421 cgagcggagg gagagggatt cgaacccacg ttggatttct ccaaacctgt tttcaagaca
      481 ggcgccttaa gccactcggc catccctcct ggcgcaaaaa ttctaccaca tccgccccct
      541 cctgccaact tcagcgcccc gggctttgtg ctatacttcg gggtatcccc ctgctggaag
      601 gatttgcatc tcggccgcat gtctcgcctg gtccgttcca ccctgatcgt tgtgatattt
      661 tttggcctgg agaaaggtct gggatttatc cgccaggttg ccatcgccca ccagtttggc
      721 ctctcccccg aattagatgc ctttaatgcc gccaacaata tccccgactt gatctttgca
      781 ctgatctccg gcggagccct ggcaatggcc ttgatccccg ttctctcgga gacaattcaa
      841 aaacacagcc ggtctgacgc ctgggagctt ttctcacgcg tcgccaacct ggtgttcctg
      901 gtcacagcgg gattatccgt gattgtagcg ctgctggccg aggctttggt cggctggcgg
      961 ttgggcattg ccccgggttt ctccactcct cagcaagagc tggtcgccga tctgatgcgc
     1021 ctgaacctgt ttgcaaccat gctgctttcc ctgggcgggc tggtgatcgc cggtctgcag
     1081 tccaaccagc attttctctt gcctgcctta gcccccagca tgtacgacct gggtatgctg
     1141 tttggggtgt tcattctctc accagagcag ggctaccagc ttggctcgct aaccctgcct
     1201 gcttttggga tgggaatcca tggcctggtg tatggtacaa tcctggggtc ggccctgttt
     1261 ctgctggtcc agattccagg gctgctccgg ttcggctttc gctggaagcc tattctgggt
     1321 ttattcaacc ctgcggtcca gcaggtactt agcttgctcg ggccgcgcgt ggcgcatatg
     1381 tttttcattc aggtgacctt cctcggccag gataatatcg cgtcgcgcct gaatgccggg
     1441 tcaatttctg ctctggtgta cggctggttg ttcatgcagg ttcccgaaac tctgatcggc
     1501 accgctttgg gcaccgtcct cttgcccacc atatccgagc agttcgcgcg ccaggacctg
     1561 gataaattcc agaccaccct caaccatatt attcgcttga tcctggccct caccatcccc
     1621 agcgcagtcc tggtcggcat ggtccttccg cccgtggtag ccatcctcgg cctcgatgct
     1681 gcgggcacca atcttgtcgt ctggaccgca cgggcgtacc tgctgggcct gatgggacat
     1741 tcactgctgg aagtggccgc ccgggctttt tacgcccgcc aggctccccg cacacccatg
     1801 ctcagtgctg ctctgaacgc ggctgttttt ctaagcctgg ccgctgttct ggcctcaccc
     1861 ctgggagcgc cggggatcgc tctggcaaat tcaattgcct ttacatccca ggcattactg
     1921 ctgcttttca tcctgcggaa gagcttcccg ggcctgctca aagtcggcgg cactctcgtt
     1981 cgggttgtgc tcatcagtgc agtggccggc gcagtcgtat ttggactgct ccagctcagc
     2041 ctccctgccc tgcccctggc agccctggca ctgctggccg gcactgtgat cgtcattcca
     2101 ttcatcttac cggagataaa attactgctg cgcctatgac aacctacaac tttgaccaca
     2161 caccggatcg ccgcggcctc gattgtgcca aatggggata ttatgccccg gatgtcatcc
     2221 ccctgtgggt agctgatatg gattttcgcc ctccgcagcc agtcctcgat gcgctccacg
     2281 atcagatcga taccgtcgga ctgggttatc cccttgcaga tggcaatgat gacctcaccc
     2341 gactgcgggc cctgttggtc gagcgcatgc agcacctgta ccactggcag atcaagcccc
     2401 aggatatcgt attcgtgccc ggggttgtaa ccgccttcca catggccagt tatgcctgtg
     2461 tcgagcccaa tggtgcgatc ctggtgcagc cgccagttta cccgcccatc tggcatgctg
     2521 ccaggaccac cggcgtcctg gcccaggaag ccccccttgt ccagctgcca ggaggaaatt
     2581 atgccgtcga ttgggaatgc ttcgaaggcg cgatcacccc tgaaacgcgc ctgttcctgc
     2641 tctgcaatcc gcacaatccg gtagggcgtg ttttcacccg cccggagctg gaaaaattcg
     2701 ccgagatctg cttgcgccat aatgttttga tctgctccga cgagatccac gccgacctga
     2761 tctactccgg tcactcgcac atcccgatcg cctccctggc acctgaaatt gcggagcgga
     2821 cgatcacact tatggccccc agcaagacct tcaatatggc tggtttgcag tgttcttttg
     2881 ccatcattcc caatgcggag ctacgcaaac aattccaacg ctctaataag ggcctggcga
     2941 gctgggtaaa taccctcgga cttgtagccg cccaggctgc ctatgcggaa ggtcaggagt
     3001 ggctggacca ggtactggtg tacctgcaag gcaaccgcga ttttctggcc cggtttgtac
     3061 aggaacgcct gccaggcatc cacatggtga aagcggaagg gacctatctg gcctggttgg
     3121 attgccggga gcttgccctg cccggaggac cgtacgcctt cttcctggag aaggcccgcg
     3181 tcgctctcaa tgatgggcag acatttggac ccggaggcga gggctttgtg cgcctcaact
     3241 ttgcctgcac ccgcgccaca ttggaacagg ctctgcttca aattcagcag gctgtagctg
     3301 gcacattcga tcaggctgct tcatagcgga attcaacgcc ccgatgcgca tttatccaat
     3361 cctctcgatc ctgatcactt tactggcttt cctgacagcc aatgtatatc cggtcaggat
     3421 tccatcggag ccagcctctc cggaatggca gagccgggtt gatccgtggg tattggaaaa
     3481 cgcagccagt ggcgagacgg aatttcttat ctccttaaat gaacaggccg atctgagcgg
     3541 agcgctcggg ttgaaaaccc aggcagccaa aggccgcttg gttgttgaaa ccctggtcga
     3601 aacagcccgg cgcacccagg cacccatcct gcgcaggctg gaagaagccg gagccacata
     3661 caaggcttac tggatttcca atctgatctg ggtgcgcggt gaggccgggc tgatccagga
     3721 gctggcatcc cgcccggacg tagcccatct ctacgccaat ccctgggtag agctcgacat
     3781 cctgccggac aaaccggtgg atgagatcgc caggccagca gccattaata ctatcgaatg
     3841 gaatattgcc agagtaaatg cggatcaagt ctgggcggca gggttcaccg gtcagggggt
     3901 agtcatcggc gggcaagata ccggctacga gtgggaccat cctgccctga aaaaccagta
     3961 ccgcggctgg gacagcagta cgcagacggc aaaccatgct tacagctggc atgacgccat
     4021 ccaaagcggc agcggcaatg aatgtggctt cgattcacct ttcccttgcg acgatcgggg
     4081 acatggtacc cacaccatgg gcatcatggt cggcgacgat ggctctggaa accagattgg
     4141 catggcgccc ggagcgcgct ggatcggctg ccgcaacatg gaaaatggct tgggcagccc
     4201 acagacttac ctcgaatgct accagtggtt tgtagcccca actgacctga acaataccaa
     4261 tccccgcccg gagctcgcgc cccaggttat taacaattcc tggagctgcc ctccggaaga
     4321 gggctgtacc gacccgctgg tgctccagca ggtcgtgcaa aatgttcacg cggcaggcat
     4381 cctcacagtc cactcagccg gcaattccgg tccaacctgc agcacagtga acacccccgc
     4441 agccatttac gccgaatcct tcacggtagg caatacgact cctgatgata ctatttcacc
     4501 ctccagcagc cgtgggccgg tcaccgtcga tggcagcgag cggctgaaac cagatatttc
     4561 cgccccggga accaatatcc gctccagtta tgctggaggg ggttataccc aacactccgg
     4621 caccagcatg gccgggccgc acgttgccgg gctgggcgcg ctgctgattt ctgcgaatcc
     4681 actcctgcgc ggtcaggtgg acgagctgga gaccatcatt cagcagtcgg ccgttccact
     4741 caccccaacc accgcctgcg gagaggatat cgccggagtc agcgtcccaa acaatactta
     4801 cggctggggg cgaatcgatg ctcttcacgc ctatgaatat tatcaattgg aagtatccgc
     4861 cacttcatca gcctcagtta tttccccggg tgaggtcatc acctataccc tgtcagctgc
     4921 gaattccggt gaagccgggt tatcctccat aaccctgatt aatgaacttt ctgcaggaat
     4981 aaccttccag aacgccagca atggaggggt tcttgatggg aatcaaataa tctgggcgtt
     5041 gggcaatctt gcagtcggtg agatacgcca ggtgcagttc agtgtttcga ctccaaccga
     5101 tcgaattgga cccatcatca acgccaatta ttacgccagt agcgatcagg tcatctccgc
     5161 ccacggtcct ccgcttagcg tgtgggcagc ccatggcatt ctctaccagc cgctcatacc
     5221 ctaccttgcc ccttaacgaa aatagcggac cagtcctatc cgcctggtcc gctattttta
     5281 ctcagtcgtt ttgattaacc tgcaagactc tctgggactg tcttttcaac ctgcccgcca
     5341 gccatcatca agcccagttc ctcgcggctc accgcctccg catccaccac atccacaatc
     5401 tgcccgtgat acatcaccgc aatgcgatct gaaagcgagg taatttcatc cagctcggcg
     5461 gagatcaata agacggctat cccttgatcc cgcttcgcca caatttgccg gtggatatat
     5521 tcgatggagc ccacatccag gccccgcgtt ggctggttgg cgatcagcat atggattggg
     5581 cggctgaatt cgcgagccac gatcaccttc tgctggtttc cgccagacag tgtgctggct
     5641 ggcagcgtat agctcggcgt gcgaacatca aattctttaa ccaggcgttc ggcattctct
     5701 ttgactgcat cctgatcgag gatcaagccc ctggagaaag gttcatgata ataggtattt
     5761 aataccaggt tatcagcaat cgaatgctgg agtaccagcc cgtgtttgtg gcggtcctct
     5821 ggtacatgtg ccatacctgt ttcaacaagg gtccgcgggt ctatggccgg aacttgctca
     5881 ccatttaact cgatggaacc cgatttcagg ctccgcaagc cggtcaaacc ttccgctaat
     5941 tcggtctgac cgttgccttg cactcccgcg atccccagga tttccccggc gaacacctgg
     6001 aacgaaacat cccgcacgac cggatgcccc tggtcatcat tcacgttcag attcttgacg
     6061 tccagcacaa ctttacccgg tttggccgga cccttttcaa cccgcaggag tacttcgcgg
     6121 cccaccatca tggccgccag gcgctgctcc gttgtctcct ggggagtagt ggtgccaacg
     6181 acttctcccc ggcgcattac cgtaatccgg tcagctaccg ccagcacctc tttgagcttg
     6241 tgggtgataa aaataaccga tacaccccgc tcagttagct ggtggatgat ccggaacaaa
     6301 tcctccactt cctgcggcgt caggactgcg gtaggctcat ctaaaaccag gattttcgcg
     6361 ttgcgatata acgctttaac gatctccacc cgctgctgga tgccaactgg caattcctcg
     6421 acaatcgcat cgggatcgac atccagacca tattcctgcg aaacccggcg aatattgtca
     6481 cgggacgtat tcaggtcgag cgctaccccg cgcgtaatct catcgcccag aatgatattt
     6541 tcagtaactg tgaataccgg cacgagcata aaatgctggt gtaccatccc aatgccatgt
     6601 tggattgcat catggggggt acggatttca acctttttcg cctcgccctc tccttgccca
     6661 tccatataga tttcaccttc atctgggctg tagaggccgt aaatgatatt catcagggtg
     6721 cttttgcctg cgccattttc acccaataat gcatggatct cgcctttgta caggttgaaa
     6781 ttgacccggt tgttggccaa aacccccgga aagcgcttgg taatattacg cacttccaat
     6841 gctaaaccat catccataag cctacctcgc tattctttcg tgtacggttc accttcagcc
     6901 gcaggggcac gcgaccgccc cacaaaacca gctaaaacaa taatggtcat cagataaggc
     6961 agcatcccaa tgaattgatg cgggattgcg atctgccctc caaattgaag ctgggtttgt
     7021 aacgccgagg taaatccaaa cagcagcgct gccccccatg caccaaatgg cgtccactta
     7081 ccgaagatca ttactgccag ggctacaaac ccgcgtccgt tggtcatgcc tcgctcaaag
     7141 gaaccaaccg cttccagggt caggaatgcc ccggcaagcc ctgccatcat gcccgcgatg
     7201 atagtattga catattggat tttatagaca ttaatgccca cggtatccgc tgcacgcggg
     7261 tgctcgccca ccgcacgggt gcgtaagccc caggctgttc g
//
LOCUS       DATMXK010000182         3052 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_10299, whole genome shotgun sequence.
ACCESSION   DATMXK010000182 DATMXK010000000
VERSION     DATMXK010000182.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3052)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3052)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3052
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10299"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1128)
                     /locus_tag="VN363_03415"
     CDS             complement(<1..1128)
                     /locus_tag="VN363_03415"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class III-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ83586.1"
                     /translation="MSDLSNDEILPLSLEHSFWTWSAQGKVGPIPLDHAEGVYFWDVN
                     GNRYLDFNSMVMCVNIGHGNQHVIEAMIEQARTLPFAAPSMATRPRARLGRLLSEITP
                     GNLDHFLFTLGGADANENAVKLARAYTGKHKILARYRSYHGATAGAMALTGDPRRLVW
                     EPNLMPGVVHFLDPYRYRSTFHINRPEVSEAEFTGDYLAHLEEIINFEGPETIAAVLM
                     ESVTGTNGVIIPPQGYMQGVRELCDRYGILMIADEVMSGFGRTGKWFAIQHWGVTPDL
                     MTMAKGLTSAYAPLGAVAMRPEIAEFFQERVYQGGLTYNSHPISLAAAIANIEVLRDE
                     HLVERAAAMGPVLKRQLTDLGEQHPSVGDVRSIGLFGVIELV"
     gene            1338..1796
                     /locus_tag="VN363_03420"
     CDS             1338..1796
                     /locus_tag="VN363_03420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83587.1"
                     /translation="MYVTVLAIHNIVRWVIVILGVLAVLRAFIGWLGKQAWIPADRKI
                     GSFIAMALDIQVLLGLILYIFFSPFGLQAFRNSNFSSIMGQREISFFAIEHPLIMILA
                     VVFAHLGVSQPRKAEKPNKKHRLAAIWFGLALVLILLGMPWARPLFPAFG"
     gene            1809..2414
                     /locus_tag="VN363_03425"
     CDS             1809..2414
                     /locus_tag="VN363_03425"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWQ83588.1"
                     /translation="MTLPGSSGVSRSLRQEILTTARGLFIEYGYRGLSMSVLARAVGV
                     SKAALYYHFQDKEQLFLAVLNELLDEIEILVDQAISDQTDSRLQIQLLVKLILSRPRD
                     ERAAIRLASQEMAYISTEASQIFYRQYQEKFIGKIEGIISSGIRAGELRPVDPSLATW
                     ALLGMMFPYFYPAQAGESPLSKTIPEQLLAIYLGGLAIWTK"
     gene            2432..>3052
                     /locus_tag="VN363_03430"
     CDS             2432..>3052
                     /locus_tag="VN363_03430"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWQ83589.1"
                     /translation="MNLTYRQSQIETASAIALWAVSFPLIKLALREISPVTLIVIRFS
                     LGGLSLFLIASRQRLWREVRWVELPAILGLGLVGVTLHQFLQVTGQVTASAGIAAFLA
                     STAPAFIVLMGSMFLKERLGLLQIGGVLLATAGAAVVSTGGFSGWQKGGQLFEPGSLL
                     ILGSAVIWAVYSILNRVLGSHRPPLVTASGMMLAGVFFSLPVWINQR"
BASE COUNT          678 a          832 c          809 g          733 t
ORIGIN      
        1 aaccagctcg atgaccccaa ataaaccaat tgaacgcaca tcgcctaccg aaggatgctg
       61 ttcgcccaaa tctgtcagct ggcgcttcag aacaggtccc attgctgctg cacgttcgac
      121 cagatgctca tcccgcaaaa cttcgatatt tgcgatagcg gcggccagcg agatagggtg
      181 cgagttatag gtcagaccgc cctggtaaac ccgctcctgg aaaaattcgg cgatctccgg
      241 gcgcatggcg accgctccca gaggcgcata agcggaggtc aggcccttcg ccatggtcat
      301 cagatcgggt gtgactcccc aatgttggat ggcgaaccat tttccagtcc gtccaaagcc
      361 gctcatgact tcgtcggcta tcatcaagat cccataccgg tcacacagct cgcgcacacc
      421 ttgcatatag ccctgcggtg gaataatgac cccgttcgta ccggtaaccg attccatcag
      481 cacggccgcg atggtttccg gtccctcgaa gttaatgatt tcttccagat gggccagata
      541 atctcctgta aactcagctt ctgagacttc agggcggttt atatgaaatg tcgatcgata
      601 gcggtatgga tcaagaaaat gaacgacgcc cggcattaaa tttggctccc agaccagacg
      661 gcgcgggtcg ccggtaagcg ccattgcccc ggcagttgcc ccgtggtaag agcggtagcg
      721 agccagaatt ttatgtttgc ctgtataagc ccgcgccagt ttgacagcat tttcgttggc
      781 gtcggcgccc ccaagagtaa ataagaaatg atccaggttt ccaggggtga tctccgacag
      841 gagccggcct aacctggccc gcggtctggt tgccatggac ggcgctgcaa aaggcagggt
      901 gcgagcctgt tcaatcatag cctcgatcac atgctggttg ccatgcccaa tattgacgca
      961 catcaccatg gaattgaaat ccagataacg attgccattt acatcccaaa aatatacacc
     1021 ttccgcatgg tcaagcggga tgggaccgac cttcccttgg gcagaccagg tccagaaact
     1081 atgttccagt gaaaggggca agatttcatc attggacaga tcagacatgt tcgcttcctt
     1141 gtttttgaga tcggctctga tcgggttatt cggaccgtac gcccgaatcc tatcacaaaa
     1201 ccaatcatcg agatggaaac aggattattt acataacacg aatagacaat tccaggttgt
     1261 tctggtaata ttacgaagct tactgaccgg tcggtaagtt agatacactt tccaaatcat
     1321 cacaggagga ttgactgatg tatgtaaccg tcttagcaat tcacaacatt gtccgttggg
     1381 tgattgtcat cctgggcgtt ctggcagtcc tgcgtgcctt catcggctgg ctcggtaaac
     1441 aggcctggat ccctgctgat cgcaaaatcg gctcatttat tgccatggca ttagatattc
     1501 aggtgctgct tggtttgatc ctgtacatat ttttcagccc atttgggctg caggctttca
     1561 ggaattccaa tttcagcagc atcatgggtc agcgcgagat cagctttttc gcgattgagc
     1621 atccattaat catgatcctg gcagtcgttt ttgcccatct aggcgtcagc cagcctcgca
     1681 aagcggaaaa acccaataaa aagcatcgcc tggcagctat ctggttcggg ttagccctgg
     1741 tgctgatttt gctgggcatg ccctgggcgc ggccgctctt ccccgccttt ggctaaggaa
     1801 cgctgccaat gaccctgcct ggatcgagcg gcgtttccag atccctgcgc caggagatcc
     1861 tgaccaccgc ccgcggtctg ttcatcgaat atggctatcg cggcctgtcg atgagcgttc
     1921 tggcgcgggc ggttggcgtt tcaaaagctg ccctatacta ccatttccag gataaagaac
     1981 agctttttct cgcggtcttg aatgagcttc tggatgaaat cgagatcctg gtcgatcagg
     2041 cgatatcaga tcaaaccgac agcaggcttc aaatccagct gctggtgaag ctgatcttat
     2101 ccagaccgag agatgagcga gctgccatcc ggctggccag ccaggaaatg gcctacatca
     2161 gcaccgaggc cagccagatt ttttatcgcc aataccagga aaaatttatc ggaaagatcg
     2221 agggcatcat ctcaagcgga attcgggccg gcgagctgcg ccctgtcgat ccgtcactgg
     2281 ccacctgggc gctgctgggg atgatgttcc cttattttta cccggcccaa gccggtgaat
     2341 caccactatc taaaacaatc cccgaacagc tcttagcaat ctatctgggc ggcctggcca
     2401 tatggacaaa gtagaatagc ttgatttcag gatgaacctc acctatcgcc agagccagat
     2461 tgaaactgct tccgccattg ccctgtgggc ggtttcgttt ccattgatca agctggccct
     2521 acgcgagatc agcccggtta cattaattgt gatccggttc agtctgggtg gtctaagcct
     2581 gttcttgatt gccagccggc aaaggctgtg gcgggaagta aggtgggtgg aactccctgc
     2641 catccttggc cttgggttgg taggtgtcac cctgcaccag ttcttgcagg tcactggtca
     2701 ggtaaccgcc tccgccggca ttgccgcgtt cctggcgtcc acagcgccag cgtttattgt
     2761 tttgatggga agcatgttcc tgaaagaacg cctgggtctg ctccagatcg ggggtgtcct
     2821 gctggcaacg gcaggggcag ctgttgtatc cacaggcggc ttctccggct ggcagaaagg
     2881 gggtcagcta tttgagccgg gcagcctgct aatcctgggc tcggcggtca tctgggcagt
     2941 ctactcaatt ttgaaccggg ttttgggcag tcatcgccca ccgctcgtca cagccagtgg
     3001 gatgatgctg gccggcgtgt tcttcagttt acccgtctgg ataaaccaga gg
//
LOCUS       DATMXK010000183         3209 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_32529, whole genome shotgun sequence.
ACCESSION   DATMXK010000183 DATMXK010000000
VERSION     DATMXK010000183.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3209)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3209)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3209
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32529"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..356
                     /locus_tag="VN363_03435"
     CDS             <1..356
                     /locus_tag="VN363_03435"
                     /inference="COORDINATES: protein motif:HMM:NF017262.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CopD family protein"
                     /protein_id="HWQ83590.1"
                     /translation="IQHRLDPIAWLCLAVLVATGLFQMSANPNYTGFLAVDNRWAAAI
                     LIKHLVIGIMVAVSAYQTWVILPGLRRIALLQARGRETPEIDTLRKREAVLLTVNLVL
                     AGIVLALTAAARSVG"
     gene            complement(369..1382)
                     /locus_tag="VN363_03440"
     CDS             complement(369..1382)
                     /locus_tag="VN363_03440"
                     /inference="COORDINATES: protein motif:HMM:NF012335.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding alcohol dehydrogenase"
                     /protein_id="HWQ83591.1"
                     /translation="MDNRAVYFTAPGQVEVRAAPLPSLQSGEILVKTLLSAISPGTEK
                     LIYQGLFPDDLLIDDSLPGITKEFCYPLTYGYAAVGRIVDAGHEVDQSWLDRLVFAFQ
                     PHQAYCISRISDIHPVPPGIKLEDAVFLANMETAVNLVMDGAPLIGENIAVCGQGVVG
                     LMTTALLSRFPLSNLITLDPISSRRQVSLNFGATASLTPESLESMQVFESLAKGGADL
                     TFELSGSPEALNLAIQITGFSGRIVIGSWYGQKRASLDLGGRFHRSRIRLISSQVSTL
                     APALSGRWDKSRRFDLAWEMIPKICPGQLITHQLPLDSAPQAYRMLAEEQDETLQIVF
                     TYD"
     gene            complement(1375..2136)
                     /locus_tag="VN363_03445"
     CDS             complement(1375..2136)
                     /locus_tag="VN363_03445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00227.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008703 -
                     5-amino-6-(5-phosphoribosylamino)uracil reductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWQ83592.1"
                     /translation="MYPFETWLSRAQEHLEKTGRPLVSLSYAQSLDGSLSDQPGKVFG
                     ISGPQSLALTHQLRSLHEAILVGVGTVLVDDPRLTARLSIEKEQSASRTHIQPRPIVV
                     DSGLRTPPTARLIQAAEKTPWIAAVAPLPADRREALHTAGAEILLLPADEHGNVSLPD
                     LLACLGQRGISSLMVEGGARILQSFLQQHLADQVIVTIAPIYLGGIPIIEPWQRSIQT
                     PLRLAEVGCQLFGEDIVLWGQLNPWHQTIDEGNLG"
     gene            complement(2123..2767)
                     /gene="ribA"
                     /locus_tag="VN363_03450"
     CDS             complement(2123..2767)
                     /gene="ribA"
                     /locus_tag="VN363_03450"
                     /EC_number="3.5.4.25"
                     /inference="COORDINATES: protein motif:HMM:NF001591.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003935 - GTP cyclohydrolase II activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase II"
                     /protein_id="HWQ83593.1"
                     /translation="MSKLTVKRMVSTRIPTQEGEFQLLLYANNHDQKEHLALIMGEIE
                     GKSDILVRLHSECFTGDVLGSHRCDCGEQLHQAMKLIAEAGTGVVVYLRQEGRGIGLL
                     DKLRAYNLQDEGYDTVDANILLGHQADERDYTVAARILEDLGVKSISLLTNNPTKIEH
                     LRRLGIRVKERVELEPVIYADNFNYLLTKVQRMNHLLNLDPFSIPANQGKNVSI"
     gene            complement(2779..>3209)
                     /locus_tag="VN363_03455"
     CDS             complement(2779..>3209)
                     /locus_tag="VN363_03455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83594.1"
                     /translation="PGGILAIVGSDPHNQSQRGQWYVYEYFEGVLQSDLERFPSWGQV
                     LNWMIGAGYANVCWQPVEILNNTWQGEDVFTDPFLKKEATSQLSLLSDGAYQSGLNRI
                     RAAIAAAQTSGTEIEFHSIIHNDMLLGRKPPDVSVVYHPA"
BASE COUNT          720 a          860 c          885 g          744 t
ORIGIN      
        1 caattcagca tcgtctcgac ccgatcgcct ggctgtgcct ggccgtgctg gttgcgacgg
       61 gtctgttcca gatgagcgcg aatcccaatt acaccggctt tctggcggta gataaccgtt
      121 gggcggctgc catcttgatc aagcatcttg tcattgggat tatggtagcc gtgtctgctt
      181 accagacctg ggttatcctg ccaggcctgc gccggatcgc attactccag gcgcggggga
      241 gggagacgcc tgaaattgac acattacgca agcgcgaagc tgttctgtta acggtaaatt
      301 tagtgcttgc cgggatagtt ctggctctga cagcggctgc tcgttccgtg ggttagcgtc
      361 taatttattc aatcgtacgt aaagacaatc tgtaaagttt catcctgttc ttcagccagc
      421 atcctgtatg cctgaggggc gctgtccagc ggcaactggt gtgtgatgag ctgcccaggg
      481 catattttag ggatcatctc ccaggcgagg tcaaaccgcc gggatttatc ccaacgccca
      541 ctgagagccg gggcaagcgt gctgacctgg ctgctgatca gcctgatccg gctgcggtga
      601 aagcgcccac ccaggtccag agaggctcgt ttttgtccgt accaggagcc gatgacaatc
      661 cgcccggaga aaccggtgat ctggatcgcc aggttgagcg cctccgggga tccggacagc
      721 tcgaaagtca gatcggctcc acccttagcc aagctttcga aaacctgcat actctcgaga
      781 gattcgggtg tcaggctggc tgtggcgccg aagttgagcg aaacctggcg gcgagaggaa
      841 attgggtcga gagtgatcag gttactcagt ggaaaccggc tcagtaaagc tgtggtcatt
      901 aaacccacca ccccttgacc acaaacggcg atattttcac caattaatgg tgcgccatcc
      961 attaccaggt ttacggcagt ttccatattc gccagaaata cagcatcttc cagtttaatc
     1021 cctggcggta ctggatggat atctgagatg cgggaaatac aataagcctg gtggggctgg
     1081 aaagcgaaca ccaggcgatc caaccaggac tggtcaacct cgtgacctgc atcgacgatc
     1141 ctccccacag cggcatagcc ataggtaagg ggataacaaa attccttcgt aatgccaggt
     1201 aaactgtcat cgatgagcag atcatctggg aaaagaccct ggtatatcag cttttcggtg
     1261 cccgggctga tcgcactcag cagggttttc accaggatct ccccagactg cagggatgga
     1321 aggggtgcag cgcggacttc aacttgcccg ggtgcagtaa agtagacagc ccgattatcc
     1381 aaggttgccc tcatcaattg tctggtgcca ggggtttagc tgcccccaaa ggacgatatc
     1441 ttcgccgaac agctggcagc ccacctccgc cagccttaac ggtgtctgga tgcttctttg
     1501 ccagggctct ataatcggaa tgccgcccag gtagattgga gcgatggtga caattacctg
     1561 atcggccagg tgctgctgaa gaaaactctg cagtatgcgt gccccacctt caaccatcag
     1621 gctggaaata ccccgctgcc ccaaacaagc cagcagatct ggtaaggata cattcccatg
     1681 ctcatctgcc gggagcaata atatctctgc cccggcggta tgcagggctt cacgacgatc
     1741 agctggcagg ggggcgacag ccgcgatcca gggagttttt tcggcggcct ggatcaggcg
     1801 tgcggtggga ggggtacgca ggccgctgtc tacaacgatg ggccgcggct gtatgtgggt
     1861 gcggctggcc gattgttctt tctctatcga gagtcgcgct gtcagacgag gatcatcaac
     1921 caggacggtg cctactccca ccaggatagc ttcatgcagg ctgcggagtt ggtgggttag
     1981 cgctaaggat tggggaccac taattccaaa aacttttccc ggttggtcgc tgagcgagcc
     2041 atccaggctc tgggcgtaac tcaagcttac cagaggccgg ccagtttttt ccagatgctc
     2101 ctgggcacgc gaaagccagg tttcaaatgg atacattctt accctggttc gctgggatcg
     2161 agaacgggtc cagattgagc aagtggttca tccgctgaac tttggtcagg agataattga
     2221 aattatcggc gtagatcact ggctcgagct caacgcgctc cttgacccgg attcctaacc
     2281 ggcgcaggtg ttcgattttg gtcgggttgt tggttagcag ggagatcgat ttcacaccca
     2341 ggtcctcaag gatgcgggca gcaaccgtat aatcgcgttc atcagcctgg tgacccagga
     2401 gaatatttgc gtccacggtg tcatagcctt cgtcctgcag gttataagcg cgcagcttat
     2461 ccaataagcc gatgccacgg ccttcctggc gcaggtagac cacgacgcct gtcccggctt
     2521 cagcgattaa cttcatggcc tggtgtagtt gttccccaca atcacagcgg tgtgagccaa
     2581 gcacatcgcc ggtaaagcac tcagaatgta agcgcaccag gatatccgat ttgccttcaa
     2641 tttctcccat gatcagagcc aggtgctctt tctgatcatg attattcgca taaaggagca
     2701 gctgaaactc accctcttgc gtggggatgc gggtggagac catccgtttg acagtaagtt
     2761 tagacatacc tcatcatcct atgctgggtg gtaaaccacc gaaacatccg gtggtttacg
     2821 gccaagcagc atatcgttat gaataatcga atgaaattcg atttcagtgc cagaagtctg
     2881 ggctgcagcg atggcagcgc ggatccggtt cagtccagat tgataagctc catcgctcag
     2941 caagctcagc tgggatgtcg cttccttttt cagaaatggg tcagtgaata catcttcacc
     3001 ttgccacgtg ttgttcaata tttcaactgg ctgccagcaa acgttcgcgt aaccagctcc
     3061 aatcatccag ttcaatacct ggccccagga tggaaaacgc tccaggtcgg attggagcac
     3121 gccttcaaaa tactcataca cataccattg gccgcgctgt gattggttgt gtggatcact
     3181 tccaactatt gccagaatgc caccgggtt
//
LOCUS       DATMXK010000184         3129 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_1814, whole genome shotgun sequence.
ACCESSION   DATMXK010000184 DATMXK010000000
VERSION     DATMXK010000184.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3129)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3129)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3129
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1814"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..330)
                     /locus_tag="VN363_03460"
     CDS             complement(<1..330)
                     /locus_tag="VN363_03460"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83595.1"
                     /translation="MNPSSRLSVLSACLILLLGFLLVGQLPALGQAGDVTGTPVPLTP
                     LDPSPIPGSPTPTLTAMPDLPGSPTHTPDQFPEITATPEPPTLTATLEITATRVSPEL
                     PTGTAEFS"
     gene            546..1253
                     /locus_tag="VN363_03465"
     CDS             546..1253
                     /locus_tag="VN363_03465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83596.1"
                     /translation="MFKKILLFGLLLGLLSGCGLINFSGGVNTPQPVSTTTVPGEIPA
                     ASATPLPEPTKTIRIPSRSTPTPGAIGAEGAAVPPQAELPVVAPVAVQVDLPLYAVQP
                     GTPAQVTNFLAPEAGCSYLGIAGQIFDERGQPVTGLIIEISGSLDGEDVLQLALSGGT
                     TRLGPGGFVIQLADRAIASQGTLSIQIFDLGGLALSDQVFFDTSAECDSNQILLNFTA
                     AQVEYDQIRYFPFLSNR"
     gene            1263..1838
                     /locus_tag="VN363_03470"
     CDS             1263..1838
                     /locus_tag="VN363_03470"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ83597.1"
                     /translation="MPGLDHFGWLAPLYERVIKPRLSDPLYEVAGLPVKGMLLDTGGG
                     TGRIAQNLVGLAGQVVVADLSLQMLKEAVKKDHLKVVNSASERMPFPDNLFERILMVD
                     ALHHVWDQVQTARELYRVLAPGGRLVIEEPDVRHWGVKLVALGEKIALMRSHFLTPPR
                     IASLFAWPGARVEILQNGATSYIVVDRSVQG"
     gene            complement(1839..2975)
                     /locus_tag="VN363_03475"
     CDS             complement(1839..2975)
                     /locus_tag="VN363_03475"
                     /inference="COORDINATES: protein motif:HMM:NF019499.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="vitamin K epoxide reductase family protein"
                     /protein_id="HWQ83598.1"
                     /translation="MIRIRHSTHLILFSTLLGLLFALSLHANANAAGPIVRGVLFYSP
                     TCGHCEKVITEVLPPLAKLYGDQLEILQIDVTMPQGQQLYQNTIQKFKITEDRTGVPT
                     LVVGETVLVGSREIPEQLPGLIESGLKEGGIIWPAIPDLEAVLPAVPVSVENSGNTQS
                     LVTATTQTAPEPDPGLSSSSGSGFTARFKQDITANTLAVIVLVAMLLSSVYVAYAFLA
                     ADPPRLPRFPAWAIPVISVLGLGVALYLSYVELYQAEAICGPVGNCNAVQNSPYAYLF
                     GVIPVGVVGAVGYIALLASYLLVDKGSESLRRTISLAMWGMAWIGTFFSIYLTFLEPF
                     VIGATCMWCLTSAILMTLLLWATTPAALAALHSDEPEEELEGVG"
BASE COUNT          691 a          832 c          950 g          656 t
ORIGIN      
        1 ggagaattca gcagtgcccg taggcagctc cggtgacacg cgggttgccg taatctccag
       61 cgttgcggtc agggttggcg gctcaggcgt agctgtgatc tcagggaatt gatccggggt
      121 atgcgtgggt gagcccggta aatccggcat agcagtcaga gtcggggttg gagacccggg
      181 aatgggggat ggatccaggg gagtcaaagg tactggtgtc ccggtcacat cacctgcctg
      241 tccgagagct gggagttgac ccaccagcag gaaaccaagc aggagaatca ggcaggcgga
      301 caggacgctt aaccgggaac ttgggttcat tcttgatcac ctcccgcaga atatggggcg
      361 gagtttatca cgggtttttt cggctgtcaa ttaaagagac taagccagtc gaaaaactct
      421 gcataactcc cacccaccag cagcaatgct tgcatatttc ttgcatctaa acctgtttat
      481 aataaggacc tcagacccgg ttccggatct ggcgggcgcc caatatgatt ggatcggagg
      541 gacgcatgtt caagaaaatt ttattattcg gcctgctgct cggactgctt tcaggttgtg
      601 ggctgatcaa tttctctggg ggcgtaaaca ctccccaacc ggtgagcacg accaccgtcc
      661 cgggtgagat tccagcggcc agtgctacac cgctgcccga gccaacaaaa accatccgca
      721 tccccagccg gagcacgcca accccgggtg cgattggggc tgaaggtgcg gctgtcccac
      781 ctcaggcaga gttacccgtc gtggcaccgg tggctgtgca ggttgatctg cctctctacg
      841 cggttcagcc gggtacaccg gcccaggtga cgaattttct cgcacctgag gcaggctgca
      901 gctacctggg aatcgccggg cagatatttg atgagcgggg ccagccggta acgggattaa
      961 ttattgaaat ctcaggcagc ctggacgggg aggatgtgct gcagctggcg ctgagcgggg
     1021 gtacgaccag gctgggcccg ggaggatttg tgattcagct ggcagaccgc gcgatcgcat
     1081 cgcagggaac cctttcaatc cagatctttg atctgggcgg gctggcactc tcggaccagg
     1141 ttttcttcga taccagtgcg gaatgtgact ccaaccagat cctgctgaat ttcaccgctg
     1201 ctcaagtcga atacgaccag attcggtatt tccctttcct ctccaatcgc tgacgcctgg
     1261 atatgcctgg gctggaccat tttggctggt tggctcccct ttatgagcgt gtgatcaaac
     1321 cgcgcctatc tgacccttta tatgaggtag cgggcttacc ggtgaaggga atgctgctcg
     1381 atactggggg tggaaccggg aggattgctc agaacctggt aggactggcc ggccaggtgg
     1441 tagtcgctga cctctcgctg cagatgttga aggaagccgt taaaaaagac cacctgaagg
     1501 tggttaactc agcgagtgaa agaatgccct tcccggataa tctgtttgag cgcatcctga
     1561 tggtcgacgc cctgcaccac gtctgggatc aggtacagac tgccagggag ctctaccggg
     1621 tgctggctcc gggcggcagg ctggtgatcg aggagccaga tgtgcgccat tggggggtta
     1681 aactggtagc gctgggtgaa aaaattgccc tgatgcgcag tcattttctt accccacccc
     1741 gcatcgccag cctgtttgca tggcctggcg ccagggtgga aattcttcaa aatggagcga
     1801 ccagttatat cgtggtcgac agatccgttc agggctgatc agccaacgcc ttccagctct
     1861 tcttccggct cgtcgctgtg cagggctgcc agggcagccg gggtggttgc ccagagtaac
     1921 agagtcatca agatcgccga ggtcaggcac cacatacagg tagcgccaat cacaaacggc
     1981 tcgaggaaag tcaggtagat cgagaaaaag gtgccaatcc aggccatgcc ccacatcgcc
     2041 agagaaatcg ttcgtctaag cgattctgag cccttatcaa ccagcaggta gctggcaaga
     2101 agggcgatat atccgactgc cccgaccact ccaaccggga taaccccaaa aagataagca
     2161 taaggactgt tctgcaccgc attgcagttt ccgaccggac cgcagatggc ttctgcctga
     2221 tacagctcta cataggatag atagagcgcc acacccaggc cgaggacaga aatcaccggg
     2281 atggcccagg cagggaagcg cggcaggcga ggtggatcgg cagccagaaa tgcgtaggca
     2341 acatacacgc tgctgagcag catagccacc agcacgatca ctgccagggt attggcggtg
     2401 atatcctgtt taaatctggc tgtaaaaccg gagccggagg aggaggaaag cccaggatcg
     2461 ggttctggag ctgtctgggt tgtggcggtc accagggatt gggtatttcc agaattctcc
     2521 accgagaccg ggacagccgg caggactgct tccagatccg ggatcgcagg ccagatgatc
     2581 ccaccttcct tcaagccgct ttcgatcaag ccgggcagct gttcgggtat ctccctggag
     2641 cccaccagga cggtttcgcc taccaccagg gtcggaacac ccgtccggtc ttcggtgatt
     2701 ttaaattttt ggatggtgtt ctggtagagc tgttgaccct ggggcattgt gacatcgatc
     2761 tgcaggattt cgagctggtc gccgtacagc ttcgccaggg gtggtaatac ctcggtgatc
     2821 actttctcgc aatgcccgca ggttgggctg taaaagagca ccccccggac gattggcccg
     2881 gcagcgttgg cattggcatg tagactgagc gcgaaaagca ggcccagtaa ggttgagaac
     2941 aagatgagat gggttgaatg gcggatgcga atcattcacg atgcccctgg gatatggaaa
     3001 agccgtcccc acgaccgtgg agacggcctg aatcaggcgg cctgaataag aagatattca
     3061 gatgccagat gctggacgca agcggacttg ctattacgct ttggtctcgg tagtagcttt
     3121 ttcggtgtc
//
LOCUS       DATMXK010000185         4698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_81635, whole genome shotgun sequence.
ACCESSION   DATMXK010000185 DATMXK010000000
VERSION     DATMXK010000185.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4698
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81635"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..800)
                     /locus_tag="VN363_03480"
     CDS             complement(<1..800)
                     /locus_tag="VN363_03480"
                     /inference="COORDINATES: protein motif:HMM:NF014670.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="creatininase family protein"
                     /protein_id="HWQ83599.1"
                     /translation="MTEFFTFEHLTWPEVYALPRDTPLILPLGSGYPMDTLASALGYP
                     AHAGLLPPIPYGWRGSGLAVPEAVFGALLSNLLDSLREDGFSRVYALTPPDLNLGLGD
                     SRITLLPGPPDGAEASLVPVHLPASSELDKVILLPYGHTEQHGLHLPLSVDTICIDAV
                     CQGAVSLLPDKAFNLPVFPYGVSTHRSSFAGTLSCGGRAFEDFVLAVLDNLVLRGFGR
                     FYLLSGHGGNSSFLVNVAKFAGERHPHIFCATSWLYLSSGEGAETVQRT"
     gene            complement(810..2678)
                     /locus_tag="VN363_03485"
     CDS             complement(810..2678)
                     /locus_tag="VN363_03485"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S9 family peptidase"
                     /protein_id="HWQ83600.1"
                     /translation="MPAEIPLTEKYERQGWPSLARPDLKPPTGWSLELATSVPRVYNH
                     SLSPNGEQAAFLWNREGFADVYTLPVRPGVTRWPARISLDRPNKPYWQDEIPCWSPDS
                     QDLAYTSGEAVFVAGADGKPPLKITPFSSGCSSPVWLPDGNSLIVAQEIEDFIQLWKV
                     SRDGTSAERLTRDPGDHRDAWPSPDGKTLIYTHNPGDDFRRSDIHLLKLSTGKVIHLT
                     GTPQQKDWHPRWSPDGAWIAFLSQRSGFNEIWLVSPQGGEPRQLTQAGLDLADPVWSP
                     DGTRIACTVNRLGALHLALVSLADGSLTELAASPGVYSHPSWAPDGNFITVEYEQSSA
                     PPDLYRFDLPAQTHTQLTFSNPPALQALHLLDLEPVVYPGAGGQEIHGLLYRPASSNR
                     AAIVHPHGGPADQYTYQWDIFDQYLAAKGYTILAPNYRGSTGYGVPFEHANYGDWGGG
                     DAQDCLNAATFLANIPGIDPARVGIMGASAGGYLVACCLSRDPEYRLACGVCKFGDAS
                     MYSSWALCERGTREYTEMMLGHPARYRKVYQDASPILQVDQVRSPVLVLHGLLDDIVP
                     PETGAEWVEALRKAGKTYEFKTYSGEGHGFLHRATQLDVYRRTERFLDWYLVPDRI"
     gene            complement(2702..3685)
                     /locus_tag="VN363_03490"
     CDS             complement(2702..3685)
                     /locus_tag="VN363_03490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558573.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWQ83601.1"
                     /translation="MNAVEPLVSVRGLKKHFPITQGVLIQRQVGAVYAVDGVSFEILP
                     GETLGLVGESGCGKTTVGRTILGLYPMTAGEVTIAGHDITHASRREWLTIRRKAQIIF
                     QDPYASLNPRWTVSGIIAEPLRVHKLLDTHAQQSQRVQELLELVGLNGRLLNRFPHEF
                     SGGQRQRIGIARALASDPQFIVCDEPISALDVSIQAQVVNLLEDLQNRLGLTYLFIAH
                     DLSMVRHICDRVAVMYLGGIVELGARDDVYDNPLHPYTVALLSAVPIPDPRKDRARKR
                     IILTGDVPSPIHPPSGCRFHPRCPLATAVCSVEVPVWREFTPGHWAACHNI"
     gene            complement(3699..>4698)
                     /locus_tag="VN363_03495"
     CDS             complement(3699..>4698)
                     /locus_tag="VN363_03495"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83602.1"
                     /translation="NIRSHLILSVNNLQTRFHTPEGTVYAVNDVSFDLHESETLAIVG
                     ESGCGKSVSMLSILGLIAIPPGEISHGEAIYLQRDLLKCSEKELDAIRGKEIAMIFQD
                     PMTSLNPVLTIGYQITESLRYHLAMSQPEADRRAIEMLESVGIPDAASRLNSYPHQFS
                     GGMRQRVMIAMALACSPSLLIADEPTTALDVTIQAQITELVQELRQRLGMAIIWITHD
                     LGVVAGLADRVIVMYAGYIVEEAQVFDLYEHPHHPYTMALLAALPRVDLSREQHLQSI
                     PGSPPNLLVEPVGCPFAARCEFVFERCVQERPPLFNTGAGHSSACWLDPATGKPRQ"
BASE COUNT          947 a         1338 c         1552 g          861 t
ORIGIN      
        1 gtacgctgca cggtttcagc accctcaccg gaggagaggt acagccagct ggtggcgcag
       61 aaaatatgcg ggtggcgctc accggcaaat ttggcgacat taaccaggaa ggagctgttc
      121 ccgccgtggc ccgagagcag gtagaagcgc ccgaacccgc gcagcaccag gttgtccagc
      181 accgccagga caaagtcttc gaaggcgcgc ccgccgcagc tgagggtgcc ggcaaaactg
      241 ctgcggtggg tgctgacccc gtaggggaaa actggcaggt tgaatgcctt gtctggcagc
      301 aggcttaccg caccctggca aacagcatcg atgcagatcg tatccaccga cagcggcagg
      361 tgcaggccgt gctgctcggt atgcccgtag gggaggagga tgactttatc cagctcgctg
      421 gaggctggca gatgaaccgg cacaaggctc gcctccgcgc catccggcgg acccggcagc
      481 agggtaatcc ggctgtcccc caggcccagg ttcaggtcgg gtggggtgag ggcataaacg
      541 cggctgaaac cgtcttcgcg caggctgtcg agcaggttgc tgagcagcgc cccgaagaca
      601 gcttcgggaa ctgccagacc gctgccgcgc cagccgtagg ggatgggtgg caaaagcccg
      661 gcatgagcgg gatatcccag ggcgctggca agcgtgtcca tggggtagcc gctgcccagg
      721 gggagaatta acggggtatc gcggggtaga gcgtagacct cgggccaggt gagatgctca
      781 aatgtgaaaa attcggtcat tggcaggctt cagatcctat ccgggacgag gtaccagtcc
      841 aaaaagcgtt ctgtgcgccg gtagacatcc aattgggttg ccctgtgtaa gaagccgtgc
      901 ccttcaccgg agtaggtctt aaactcatag gtcttgccgg ctttacgcag cgcttcaacc
      961 cattccgcgc cggtctcggg cggtacgata tcatcgagca aaccgtgcag gacgagcacc
     1021 ggactgcgca cctgatcgac ctgcaggata ggtgaggcat cctgataaac cttgcggtac
     1081 ctggccgggt gccccagcat catctcggta tattcacggg tgccgcgttc acacaatgcc
     1141 caggagctgt acatgctggc atccccgaac ttgcagaccc cacaggccag gcggtactcg
     1201 gggtcccggc tcaggcagca ggcaaccaga taaccgcccg ccgatgcgcc catgatcccc
     1261 acccgcgccg gatcgatgcc agggatattt gccaggaaag tggctgcgtt caggcagtcc
     1321 tgggcgtccc caccacccca atcgccataa ttagcatgct cgaaaggcac cccatagccg
     1381 gtggagccgc gataattggg cgccaggatg gtgtagcctt tcgctgccag gtactggtcg
     1441 aagatatccc actggtaggt gtactggtcg gccgggccgc cgtgagggtg aacgatggct
     1501 gcccggttgg agctggctgg ccggtaaagc aagccgtgga tctcctgccc gcccgcgccc
     1561 gggtaaacga ccggttccag gtcgagcagg tgcaatgcct gcagggcagg tgggttagag
     1621 aacgtgagct gagtgtgcgt ctgcgcgggt aaatcgaagc ggtacaggtc gggtggagca
     1681 gaggactgtt cgtactcgac ggtaatgaag ttcccatctg gagcccagct gggatgtgag
     1741 taaactcccg gtgaagctgc cagctcggtc agcgagccgt ccgccaggct gaccagcgca
     1801 agatgcagcg cacccaggcg attgaccgtg caggcaatcc gggtgccatc cggtgaccag
     1861 acaggatcgg cgagatcgag accagcctgg gtgagctggc gcggctcgcc gccctggggg
     1921 gagaccagcc agatttcatt gaacccggac cgctgggaga ggaaggcgat ccaggcgcca
     1981 tccggcgacc agcgcgggtg ccagtctttc tgctggggtg taccggtcag gtgtatgacc
     2041 ttgccggtgg aaagcttgag gaggtggata tcagaacggc ggaaatcatc cccgggatta
     2101 tgcgtgtaga ttaatgtttt accatccggc gaaggccagg catcgcggtg atcgcctgga
     2161 tcccgggtca gccgttcggc agaggtgcca tcccggctca ccttccagag ctggatgaaa
     2221 tcttcaatct cctgcgcaac gatcaggctg ttcccatccg gcagccagac cgggctggag
     2281 cagccactgc tgaaaggtgt gatcttgagc ggtggtttgc cgtctgcgcc ggcaacaaag
     2341 accgcctcgc cgctggtgta ggccaggtcc tggctgtctg gagaccagca gggaatttca
     2401 tcctgccagt agggcttatt gggacggtcg agtgagatcc ttgccggcca gcgggttacc
     2461 ccggggcgga caggcagggt ataaacatcg gcgaaaccct ctcgattcca gaggaaagct
     2521 gcctgctcgc cgttgggcga aaggctgtga ttgtagacac ggggaacgga ggtggccagc
     2581 tccaggctcc aaccagtggg gggtttaaga tcggggcgcg ccagggaagg ccagccctgg
     2641 cgctcgtatt tttcggtgag tgggatttca gcaggcatat attttatttt ccagacaggg
     2701 ctcagatatt gtggcaggct gcccagtgcc cgggggtaaa ttcgcgccac accggcactt
     2761 ctacactgca gaccgcggtt gccagtgggc agcggggatg aaagcggcag ccggaaggcg
     2821 ggtggatcgg gctgggtaca tccccggtga ggataatacg cttgcgggcg cgatctttgc
     2881 gcgggtcggg gatgggcacc gccgagagca gggcgacggt gtagggatgg agcgggttgt
     2941 cgtagacgtc gtcgcgagct cccagctcaa cgatcccacc caggtacatt accgccaccc
     3001 ggtcgcagat gtggcggacc atgctcaaat catgggcgat gaagagataa gtcagcccca
     3061 ggcggttctg caggtcctcc agcaggttga caacctgagc ctggatggat acgtccaggg
     3121 ccgagatggg ctcatcgcat acgataaact ggggatcgga agccagggcg cgagcgatac
     3181 cgatgcgctg gcgctggcca ccggagaatt cgtgcggaaa gcggttgagc aggcgaccgt
     3241 tcagtccgac cagctccaat aattcctgga cccgctgaga ttgctgcgca tgagtgtcga
     3301 gcagtttatg cacccgcagc ggctcggcga tgatcccgct gaccgtccag cgcgggttga
     3361 gcgaagcgta aggatcttga aaaatgattt gggctttgcg gcgaatggtc agccactctc
     3421 ttcgggaagc gtgggtgata tcgtgcccgg cgatggtcac ttctccggcg gtcatggggt
     3481 aaagccccag gattgtgcgc ccgacagtgg tcttgccgca gcccgattcg cccaccagcc
     3541 ccagggtttc accaggtaag atctcaaagc tgaccccatc cacggcatac accgcgccaa
     3601 cctggcgctg gatgaggaca ccctgggtga tcgggaaatg tttcttcagt ccgcggacgg
     3661 aaaccagtgg ctcaacggca ttcatcaggc agtatccatt attggcgggg ctttccggtc
     3721 gctggatcca gccagcaggc ggaactgtga ccggcaccgg tgttgaacag cggtgggcgt
     3781 tcctggacac agcgttcaaa tacaaattcg cagcgggcgg caaaaggaca gccgaccggc
     3841 tctaccagca ggttgggcgg cgagccgggg atcgactgca ggtgctgctc gcggctcaga
     3901 tcgacccgcg gcaaggccgc cagcaaggcc attgtatacg ggtggtgggg atgttcgtag
     3961 agatcgaaga cctgagcctc ctccacgata tagccggcat acatcacgat aacccggtct
     4021 gcgagcccgg caaccactcc cagatcgtgg gtgatccaga taatcgccat tcccaaccgc
     4081 tggcgcagct cctgcaccag ctcggtaatt tgagcctgga tggtcacatc cagggccgtt
     4141 gtcggctcgt cggcaatcaa caggctcggg ctgcacgcca gcgccatggc gatcatcact
     4201 cgctggcgca tgccgcccga aaactggtgc gggtagctgt tcaggcggga agcggcatcg
     4261 ggaatgccga ccgattccag catctcgatc gcccgccggt cggcttcagg ctggctcatc
     4321 gccaggtggt aacgcagcga ttcggtaatc tggtagccga tggtcaggac cggattcagg
     4381 ctggtcatgg ggtcttgaaa gatcatggcg atttctttgc cgcggatggc atccagctcc
     4441 ttttcggagc atttcagcag atcgcgctgc aggtagatgg cttcgccgtg agaaatctca
     4501 ccgggcggga tggcgatcag gcccagaatt gaaagcatcg agactgattt tccacacccg
     4561 ctttcaccca cgatcgccag ggtttcgctc tcgtggaggt cgaaggagac atcgttcacc
     4621 gcgtacacgg tgccttccgg ggtatggaag cgggtctgca ggttgttgac ggacaggatg
     4681 agatgacttc tgatattc
//
LOCUS       DATMXK010000186         2841 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_67882, whole genome shotgun sequence.
ACCESSION   DATMXK010000186 DATMXK010000000
VERSION     DATMXK010000186.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2841)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2841)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2841
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67882"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VN363_03500"
     CDS             <1..404
                     /locus_tag="VN363_03500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83603.1"
                     /translation="GFDERYPRPAGEDADITIRLRNAGYRLEFEPRAVVTHSPPRHHF
                     SDMLRHSYYQGMYSTKVDPRYAKDEGLPGVLRSRAGLILAAPVLALLVSGGIFIKYPR
                     LLKYWYTLPALYLSKIAWCWGAAHADKKSSL"
     gene            401..1261
                     /locus_tag="VN363_03505"
     CDS             401..1261
                     /locus_tag="VN363_03505"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ83604.1"
                     /translation="MNDQSKKVSVLIPCYNQAALIRPVVEAILAQTRLPDEIIVVDDA
                     SQDTSLEILRTLPVKIIQHETNLGLSRARNSAIAGAAGDILVFVDGDAIAAPDLIEVL
                     LSGYQLSDDGNLGGIGGRGIEALQLNVYDRWRAYHASQSHGNKPVDDVPFLYGLCMSY
                     TRRAIEKVGGFDPFFSQTCGEDIDIGIRLHQAGYRLGYLPQAVVYHQHSDDEKRLLKT
                     HSNWAYWNYLLKKRHSLPTWKAYAGIFRRLFTESVTDLIVRRDVELFTLDLKMFRVKL
                     SAIRKAARLT"
     gene            1370..2107
                     /locus_tag="VN363_03510"
     CDS             1370..2107
                     /locus_tag="VN363_03510"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ83605.1"
                     /translation="MPVTTLHSKQAVAPSEDELKQSYDRLYQEWMGQHRNTGVAHQML
                     DLMEVKSGSLLDVACGLGYLLDFANERGAKAYGVDLVHLPLLKAHQENPERALIEANG
                     ECLPWPDETFDYITSLGSLEHYINPDLGAREIARILKKDGKAAINLPNSHHIQAIYNV
                     YKTGGVLPDLQDYERFATRVEWQDFLENNGLRVLSVHKFNVGFSRKFKKGREGFWYLF
                     NILYRLFGDRWIPTNLSFSLTYICTKA"
     gene            2191..>2841
                     /locus_tag="VN363_03515"
     CDS             2191..>2841
                     /locus_tag="VN363_03515"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ83606.1"
                     /translation="MSKIGEIIAWRINQLFPQLEIHRSLENAKLDISANQAWSLAEAR
                     RVVKYFDPYWNLKDKHLLDIGTGIGGKLSLYQEAGVRAITGIDITPQSVSISRQYIQD
                     KVGPRYTAQEFYFSVADAAKMPFADNCFDAIVSINVFEHIMGVENAVSESYRVLKPGG
                     IAFLHVPPYYGPWGPHLENWIHFPWSHLFFSDRTLLRVAEREDKRRPLNEKFMYAAQ"
BASE COUNT          717 a          696 c          723 g          705 t
ORIGIN      
        1 gggggttcga cgagcgctac cccagacctg ccggtgagga tgcggatatc accatccggc
       61 tgcgcaacgc gggttaccgg ctggaatttg agccgagggc agtcgtcacc cattcaccgc
      121 cccgccacca cttctccgat atgctgagac acagttatta tcaggggatg tattcgacaa
      181 aagtggatcc acggtacgcg aaagatgaag gattaccagg ggtcttgcga tccagggccg
      241 gcctcatcct ggctgcgcca gtacttgctt tgctggttag cggtggaatt tttatcaagt
      301 atccccggct gctaaaatac tggtacacgc tgcccgcgct gtatctctca aaaattgcct
      361 ggtgttgggg agctgcccac gccgataaga agagcagttt gtgaacgacc aatccaagaa
      421 agtctctgtt ttgatcccgt gctacaacca ggctgccctg atcaggccgg tcgtggaagc
      481 gatcctggct caaacgaggc tgccagatga aattattgtc gtagatgatg cttcgcaaga
      541 taccagcctt gaaatattaa ggactctacc ggtcaaaatc atccagcacg aaaccaatct
      601 gggtttatcg cgggcacgca acagcgccat tgctggcgca gcaggtgaca tcctcgtctt
      661 cgtggatggg gatgcgatcg cggccccgga cctgattgag gtgctgctct ctggttatca
      721 attgtctgat gatgggaatt taggtggcat aggaggccgc ggcattgaag ccctccagct
      781 taatgtctat gatcgctgga gagcctacca tgccagccag agtcatggaa ataagccagt
      841 cgatgatgta ccctttctat atggtctgtg catgtcctac acgcgcaggg caatcgaaaa
      901 ggtcggtggg tttgacccgt tcttttcaca gacctgtgga gaagatatcg atatcggtat
      961 ccgcttacat caggctggat atcgcctggg ttatctgccg caggctgttg tttaccacca
     1021 gcacagcgat gacgaaaaac ggttattgaa aacccattcc aactgggcat attggaacta
     1081 tcttctaaaa aagcggcact cgctgcctac ctggaaagct tatgcaggta tcttccgcag
     1141 gttgttcacc gaatcagtga cagatttgat cgtgcgccgc gatgttgaat tattcacact
     1201 tgatctgaaa atgttcaggg ttaagttgtc agcaatccgg aaggctgccc ggttaacctg
     1261 atatatccgt gaaaaatcca tcccacagct gatcatcaat cagcgaaaat aatctcaagt
     1321 ataattcagc cctggatcta acgataaaag gggtcgcagg agtaatcatt tgccagttac
     1381 cactctacat tcaaaacaag ctgttgcacc ctccgaagac gagttaaaac aatcttacga
     1441 ccggctttat caagagtgga tgggacagca tcgaaatacc ggtgttgctc accagatgct
     1501 ggacttaatg gaagttaagt ctggcagcct gttagacgtg gcgtgtggtt taggatacct
     1561 gctcgacttt gcaaatgagc gcggcgcgaa agcttatggg gtcgatctcg tccatttacc
     1621 cctgttgaag gctcatcaag agaacccgga acgcgccctg attgaggcca atggcgagtg
     1681 ccttccctgg cccgatgaga cattcgacta tatcaccagt ttaggaagcc tggagcatta
     1741 catcaatcca gatcttggag cccgggaaat tgcccggatc ctgaaaaaag acggcaaagc
     1801 tgccatcaat ttacccaact cccaccatat ccaggctatc tataatgttt acaaaaccgg
     1861 tggtgtcctg cctgacttac aggattatga gcggtttgcc acccgggtgg aatggcaaga
     1921 tttcctggag aacaatgggt tacgggttct gtctgttcac aaatttaatg tggggttttc
     1981 ccgaaaattc aaaaagggac gcgaaggttt ctggtatttg tttaatatcc tgtatcgcct
     2041 ttttggagat cgttggattc ctacaaattt aagcttttcc ttgacctata tctgtacaaa
     2101 agcataatcc ttaaccaatc ctatgcatgt ttttgataat ttcgtcacgc taagctgcac
     2161 aaagggaatt tcctgggatg tagaaccggc atgagtaaaa ttggggagat catcgcctgg
     2221 cggatcaacc agcttttccc gcaactggag attcaccgca gtttagaaaa tgcaaagttg
     2281 gatatcagcg ccaatcaggc ctggtctctg gctgaagccc gccgggtcgt aaaatacttc
     2341 gatccgtact ggaacctgaa agataaacat ctcctggata tcggcacagg gattggggga
     2401 aaattaagcc tgtaccagga ggcgggcgtg cgtgcaatca ccgggatcga catcactccg
     2461 cagagtgtct ccatctcacg ccagtacatc caggataaag tcggtccacg ctataccgca
     2521 caggagtttt acttcagcgt tgcggatgca gcgaagatgc cttttgcgga taactgcttt
     2581 gatgccatcg tttcgattaa tgtctttgaa catatcatgg gtgtggagaa tgccgtcagc
     2641 gagtcctaca gggtgctaaa accgggagga atagcttttc tgcatgtgcc gccttactat
     2701 ggaccatggg gtccgcatct ggagaactgg atccatttcc cctggtcgca tctattcttt
     2761 tctgatagaa cactcctgcg agttgccgag agggaagaca aacgccgccc actgaatgaa
     2821 aaatttatgt atgccgccca g
//
LOCUS       DATMXK010000187         5478 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_154166, whole genome shotgun sequence.
ACCESSION   DATMXK010000187 DATMXK010000000
VERSION     DATMXK010000187.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5478)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5478)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5478
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154166"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..329
                     /locus_tag="VN363_03520"
     CDS             <1..329
                     /locus_tag="VN363_03520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83607.1"
                     /translation="YLVTETMMTIEVPRRRRLKLGEDLTGEFPAVLQQISSTELLALL
                     AAIDPTPDSLVESGTNYWGDLADRIHFIADMFRCFESSVELQEPPFDPEQTVAIKENR
                     LPEGRL"
     gene            complement(352..1197)
                     /locus_tag="VN363_03525"
     CDS             complement(352..1197)
                     /locus_tag="VN363_03525"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4405 domain-containing protein"
                     /protein_id="HWQ83608.1"
                     /translation="MKIKSPKSNWIMDAGLFVLLLASFWLDLTGLTFHQWLGAVLAAG
                     IGVHFLTHWSWVKTISARFFGHTSGQARRYYLLDASLLLSFTLITLTGLVISTWLGLT
                     LTNYSFWRDLHVGSSIFALLVLVVKIALHWRWVINVAHRYIISPRVSAPRPELHPAQA
                     RLAVVPVNTERREFLRLMGLVGVAALVSIANVADSDEQAVASAYADDQATGSAGDSTS
                     EIVPELPEAGSSSSISSGGLSLSSSTDPTQCRVECNKRCSYPGHCRRYVDSNGNNRCD
                     HGECI"
     gene            complement(1372..2481)
                     /locus_tag="VN363_03530"
     CDS             complement(1372..2481)
                     /locus_tag="VN363_03530"
                     /inference="COORDINATES: protein motif:HMM:NF024771.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dipeptide epimerase"
                     /protein_id="HWQ83609.1"
                     /translation="MLNPAITKIEIYKLDLPLHTPFRIATNYRTSANNILVRIHSADG
                     FYGLGEGSPAPYTAGEDQDISFAAAQRLARLLINQDSTQIETLHRLMEASLVRNTTTR
                     CAIDLALHDLLAKRLGVPLYTLLGGANRPVETDMTIGIESPDVMADRAVEIQKKGFTN
                     LKVKLGTSRLEDVARIRAIRQAVGPQMTIRLDANQGWDPAEAARILVELAPYDIQYCE
                     QPVRAWNEAAMRRLRQLSPIPIAADETLFDHRDALRLASNETCDLFNIKLAKSSGIHN
                     ALKINAIAEGAGITCMLGCMSESRLGLTAAAHVVSARPNIAHADLDSCFFHADDPVEG
                     GITYTGGTIHLPTAPGIGADFPQEYLDHLISAAIQ"
     gene            complement(2502..4217)
                     /locus_tag="VN363_03535"
     CDS             complement(2502..4217)
                     /locus_tag="VN363_03535"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83610.1"
                     /translation="MTSKKVVTFLLLTLFLAACSAPQPAATEPPSASEAVAQETAPAA
                     TPPDAAAEFNGLPLVTDRGELFSGSGVCVACHTGMVDEAGVDVSIDRSWSATMLANAS
                     KDPYYLATVRSEVTMAPDMQAVIEDKCSSCHMPMAFLTATFKDEPAAMLDAGFKSAEH
                     PLHGLAQDSISCNLCHQLEPDNFGAEASFSGGFAVDTELASGERKSYGPYVVDDQQAS
                     VMQAASGFVPVQGLHTAQSELCASCHNLVTPYLDAAGQVAGEFPEQAIYSEWQNSVYK
                     AESTCIDCHMPVAKGNVQISLVGGPPRSPFSRHVFAGGNAFMLSMLRENGEEIGITAR
                     PADLDQSIQAASDIIGKQSATLAVENLKLDGTALTGEVVITSQTGHKFPAGYPSRRAW
                     LHITVKDASGATVFESGAVNEDGLITGNDNDADPAVYEPHYTALTTPDQVQIYETILI
                     NSDGEVTTKLLRSAAYAKDNRLLPVGFDLKKATQDTLVFGDAAEDPDFAAGGDKLSLQ
                     IDLGSAAGPFTIHAELLYQSIGYRWAQNLMLEENPEAADFSGYYATVPNLPLVAAQAE
                     ATVAP"
     gene            4345..5445
                     /locus_tag="VN363_03540"
     CDS             4345..5445
                     /locus_tag="VN363_03540"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ83611.1"
                     /translation="MSRRIQAGKERLQCYLSLATAPSQVKLDGKYMANDGGDPMRVLF
                     IGGTGVISSACSELAVQRGINLYLLNRGQSSRPTPAGAKIIQADIRQTEETARALSGL
                     DFDAVVDWIAYTPEQVQADIELFRGRTRQYIFISSASVYQKPPRTLPITESTPLHNPF
                     WEYSRLKAACEAVLMDAYRQEDFPTTIVRPSHTYDRTLFPFTGGYTQVGRMRAGKPVV
                     VHGDGTSLWTLTNHRDFAVGLVGLLGHPLALGEAFHITSDEWLSWNQINEIMAEAAGV
                     KPRLVHAPSELIARYDARWGESLLGDKSFSALFDNSKIRRLVPEFKPVIPFWQGAREI
                     MAWYDADPSRQVINSYVDAAQDQIIQFLDRRL"
BASE COUNT         1296 a         1411 c         1646 g         1125 t
ORIGIN      
        1 attacctggt caccgaaaca atgatgacga tcgaagtgcc gcgcagacgc aggctaaaac
       61 tgggggaaga cctgaccggc gaatttccgg ctgtgctgca gcagatcagc agcaccgagc
      121 tcctggcgct gctggcggcg atcgacccga cccctgacag cctggttgaa tcggggacca
      181 actactgggg agacctggcg gaccggatcc atttcattgc cgatatgttc cggtgctttg
      241 agagctcggt ggagctgcaa gaaccaccgt ttgatccaga acagaccgtg gcgattaagg
      301 aaaaccggct gccagaggga cgtttataga gcactctagc ctgggagtgg gtcaaataca
      361 ttcgccgtga tcacagcggt tgttcccatt gctatccacg tagcgccggc agtgcccggg
      421 ataagagcag cgcttattgc actcgacccg gcattgagtg ggatccgtag atgaggacaa
      481 cgacaggcct ccgctcgaaa tggatgagct gctgccagcc tcgggaagtt caggcacgat
      541 ttcagaggtt gaatcgccgg cactccccgt agcctgatcg tcagcgtaag cgctggcaac
      601 cgcctgctca tccgaatcgg ccacgttggc aattgacacc aaagccgcaa ccccgaccaa
      661 tcccatcagg cgcagaaatt cacgccgctc ggtattgact ggcacaactg ccagcctggc
      721 ttgagccgga tgcagctccg ggcgtggggc agaaacccgg ggggaaatga tatagcggtg
      781 agccacatta atgacccaac gccagtgcaa ggcaatcttt accacgagaa ccagcagggc
      841 gaagatcgag ctgcccacat gcaggtctct ccagaaacta taattcgtta aagtaagccc
      901 gagccaggta gaaatgacca gcccagtcag agtaatcagg gtaaaactga gcaaaagact
      961 ggcatcaagc aggtaatagc ggcgcgcctg cccggaagta tgcccgaaaa agcgggcgga
     1021 tatggttttg acccagctcc agtgggtgag aaaatgaacg ccgatacccg cggcaaggac
     1081 ggcccccagc cactgatgga aagtaagccc ggtcagatcc agccagaagg acgccaggag
     1141 taagacaaac aaaccggcat ccattatcca atttgatttg ggagatttta ttttcatgat
     1201 gatccccttt ctttgtttaa gaagttgagg tcatcatagc gggagtgtat aaagaaaatt
     1261 tggggttttg gttaagaaaa tatgtggctt tggatgattt cgttcgggga aaggggggat
     1321 taagaaaaaa aggctgccct cagcgagcag cccaaactgg atattctatc gttattgaat
     1381 ggcagcggat atcaaatggt cgagatattc ctgggggaaa tcagcgccga tgccaggggc
     1441 ggtgggcagg tggatggtac caccggtata agtaatgccg ccctcgaccg ggtcatcggc
     1501 gtgaaagaag cacgaatcca gatcagcgtg ggcgatattg gggcgggcgg agaccacatg
     1561 ggcggcggca gtcaggccca ggcggctctc cgacatacag cccagcatgc aggtgatccc
     1621 ggcgccttcg gcgatggcgt tgatcttaag cgcattgtga atgccgctcg atttggccaa
     1681 cttgatatta aacaggtcgc aagtctcatt acttgccagg cgcaaggcat cgcgatggtc
     1741 gaagagggtc tcatcggcag cgatcgggat gggggaaagc tggcgtaagc ggcgcatggc
     1801 agcttcattc caggcgcgga ccggctgctc gcaatactgg atatcatagg gagctaattc
     1861 aaccaggatg cgggcagctt cggcggggtc ccagccctga ttggcatcca ggcggatggt
     1921 catctgcggg ccaacagcct ggcggatggc gcggatgcgg gctacatcct ccaggcggct
     1981 ggtgcccagc ttcactttga ggttggtgaa gccttttttc tggatctcca cagcgcggtc
     2041 tgccatcaca tcgggggact cgatgccgat cgtcatgtcg gtctctacgg ggcggttggc
     2101 accacccagg agggtataaa gcggcacacc caggcgtttg gcaagcaaat cgtgcagggc
     2161 gaggtcgatc gcgcagcggg tcgtggtgtt gcgcaccagg ctggcttcca taaggcggtg
     2221 gagagtctcg atctgggtcg aatcctggtt gatcagcagg cgcgccaggc gctgggcggc
     2281 agcgaaactg atgtcctgat cctcaccagc tgtataagga gcaggagagc cctcgccgag
     2341 gccatagaaa ccatcggcgg aatggatacg caccaaaatg ttgttggcgc tggtgcgata
     2401 attcgtggca atccgaaagg gggtatgcag gggaagatca agcttgtaaa tttcaatttt
     2461 cgttatggca ggattcagca ttggtacctc aagggtaaaa attatggggc gacggtcgcc
     2521 tcagcctggg cagccaccag gggtaaattg ggcacagtag cataataacc actgaaatct
     2581 gcggcctcag gattttcttc gagcatcaga ttttgagccc agcgatagcc aatcgattgg
     2641 tacaggagct cagcgtggat ggtgaaggga ccagctgcgc tgccaagatc gatttgcaga
     2701 gacagtttat cgccgccggc agcaaaatca ggatcctcgg cagcatcccc gaagacaagc
     2761 gtatcctggg ttgctttctt aagatcaaaa ccaacgggca gcagacggtt gtctttggca
     2821 taggcggcgg agcggagcag cttcgtggtc acttcaccat ccgaattgat caggatggtc
     2881 tcgtaaatct gcacctgatc gggtgtggtg agggcggtgt aatgcggctc gtagaccgcc
     2941 gggtcagcat cattatcatt gccggtgatg aggccatctt cattaaccgc acccgattca
     3001 aagacggtcg cgccggaagc atctttgacc gttatatgca gccaggcgcg acgggaggga
     3061 taccctgccg ggaatttatg cccggtctgg ctggttatta caacttcccc ggtcagagca
     3121 gtgccatcca atttaagatt ctctacagcg agggtggcag actgcttgcc aatgatatcg
     3181 ctggcggcct ggatggactg atccaggtct gcaggccggg cagttatccc gatctcttca
     3241 ccattctcac gcagcataga gagcataaaa gcattgccgc ctgcgaaaac atggcgggag
     3301 aacgggctgc gcggaggacc accaacgagt gatatctgga cattaccttt tgcgacgggc
     3361 atgtgacaat caatacaggt cgattcagct ttgtagacgc tgttttgcca ctcggaatag
     3421 atggcttgct caggaaactc accggcaacc tgcccggcag catccaggta aggagtgacg
     3481 aggttgtgac agctggcgca caattcggat tgggcggtat gcaggccctg caccggcaca
     3541 aaaccagagg cagcctgcat cacactggcc tgctgatcat ccacaacata ggggccgtag
     3601 gatttgcgct cgccgctggc cagctcggta tccacggcaa agccgccgct aaagcttgcc
     3661 tcggcgccaa aattatctgg ctcaagctgg tggcaaagat tgcacgaaat tgaatcctgg
     3721 gcgagaccat ggagagggtg ctcagcagat ttaaagccag catccagcat agccgccggc
     3781 tcatctttga aagtcgcagt cagaaaagcc atcggcatgt ggcaggatga gcacttatct
     3841 tcgatgactg cctgcatatc cggcgccatg gtaacttcgc tgcgcacagt cgcgagataa
     3901 taagggtctt tactggcgtt tgcgagcatg gtggcactcc aggacctgtc aatggagaca
     3961 tcgacaccag cttcatctac cataccagta tggcaggcca cacagacgcc ggaaccagag
     4021 aagagctcgc cgcgatcggt aaccagaggc aggccgttga attcggcggc ggcatccgga
     4081 ggggtggctg caggagctgt ttcctgggca accgcctctg atgctgaggg cggttcggtg
     4141 gcagctggct gaggcgcgga acaggctgcc aggaagaggg tgagcagtaa aaaggtgact
     4201 actttttttg aggtcatttc cactccgcca atttctgtaa catatttttt cgcggtttgg
     4261 atatgtttat aacttaagtg gatgattaag acaagatgtg agtgtgaaaa agggtgcaac
     4321 ctggtagagt ctgcctcttt gacaatgagt cggagaatcc aggccggcaa agaacggtta
     4381 caatgctacc tgtcgctggc gacagcaccc agccaggtaa agctcgacgg caaatacatg
     4441 gcaaatgatg gaggtgatcc aatgagggtt ttatttatcg ggggcacagg ggtcatcagc
     4501 tcagcgtgca gcgagctggc agtgcagcgc ggcatcaacc tgtacttatt aaaccggggg
     4561 caaagttccc gcccgactcc tgcaggggct aagatcatcc aggccgatat ccggcagacc
     4621 gaagagaccg cccgcgccct ctctgggctg gattttgatg cggtagtgga ttggattgcc
     4681 tatactcccg aacaggtgca ggctgatatt gagcttttcc gggggcggac gcgccaatac
     4741 atctttatca gctcggcgtc cgtgtatcaa aagccgcccc ggacgctgcc aatcacagaa
     4801 agcacacctt tacacaatcc attctgggag tattcacgcc taaaagcagc ctgtgaggca
     4861 gttttaatgg atgcctacag gcaggaagat tttccaacga cgatcgtgcg cccatcccat
     4921 acctatgacc ggaccttgtt cccctttacc ggggggtaca cccaggtagg ccggatgcga
     4981 gcgggcaaac cggtggtcgt tcacggagac ggcacctcgc tgtggactct gaccaatcac
     5041 cgtgatttcg cggtggggct ggtcgggctg ctgggacacc cgctggcgtt gggggaagca
     5101 ttccacatta cttccgatga atggctcagc tggaatcaaa ttaatgagat tatggcggaa
     5161 gctgccggtg tgaagccgcg cctggtgcat gccccatccg agctgatcgc ccgctacgac
     5221 gcccgctggg gggaatcttt actgggggat aaatcgttca gcgcgctctt tgataacagc
     5281 aagattcgcc ggctggtccc agaatttaag ccggtcattc ctttctggca gggtgccagg
     5341 gagatcatgg cctggtatga cgccgatccg agccggcagg tgatcaattc ttatgtagat
     5401 gcggcgcaag accagatcat ccaattttta gaccggcgtt tatagacagg cgtttataga
     5461 cagtaggcag tgaaaaca
//
LOCUS       DATMXK010000188         3019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_92052, whole genome shotgun sequence.
ACCESSION   DATMXK010000188 DATMXK010000000
VERSION     DATMXK010000188.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92052"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(7..981)
                     /locus_tag="VN363_03545"
     CDS             complement(7..981)
                     /locus_tag="VN363_03545"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWQ83612.1"
                     /translation="MKFPFTCSVIVPVYRGAETIAELAAQLVQTLEGCVPDYEIVFVN
                     DGSPDNSWEVITQLTQRYSQVSGINLLRNYGQHNALLCGIRQARGEVIVTLDDDLQNP
                     PAEIPRLLDKLAGGWDVVYGTPAQEQHGLWRDLASQVTKLALQSTMGVDTARQVSAFR
                     AFRSAVRQGFAEYHSPSVSIDVLLTWGAARFAAVPVAHHPRHAGQSNYTFRKLVRHAM
                     NMVTGFSILPLQFASLMGFLFAIFGLVVLVYVVGRYLIQGGSVPGFPFLASTVAIFSG
                     VQLFALGIMGEYLARMHFRLQDRPAYVISEQTIATNAPMPVPTPDRTQ"
     gene            complement(992..>3019)
                     /locus_tag="VN363_03550"
     CDS             complement(992..>3019)
                     /locus_tag="VN363_03550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83613.1"
                     /translation="LLFCVAAYGLLLPWLGFYWDDWPTIWYLHFWGADIFKDVFASDR
                     PLLGVLFRITSPLVGETPAAWQVFGILTRWLSGLAFWSLLRAIWPRHPQPAAWTALLF
                     CLYPGFSQQFISVTYSQVFIVLAVFLFSLTSMVWSVRRPERFWLCMMVSLACSGLVLF
                     TVEYFFGLELLRPLILWFVLREKIPEAGKRLKRILVVWAPYLLLLAAFFVWRVFLSDT
                     PRGEVRLIPRLLSDPVNALSGLLQSMGQDIFSSSVLAWGQTLQFGLLANFGLSSIVVY
                     AAIVLASVIAAALLLSAYTKGLTGDEAANTASAGSASQPYAHQNWAKDAILLGIAGLL
                     VGGWPFWGAGYPIGLDFPWDRFTLAMMFGASLLLAGVIGLLVRQPVPRLLVLSVLVGL
                     AVGLQFQHANTYRREWNTQRNFFWQWSWRAPAIQPGTTILTSELPFVHYTDNSLAAPF
                     HWLYAPEHTARAMDYMFYNIDARLGLTLAGLEAGLPIEQVYRATAFTGTTSQVLVVTY
                     QPPGCLVVYDPARHARVPQKPKYIGDVMDISDPGLILAEAARPAVPVEAYFGSEPTPN
                     WCYYYEKAELALQNRDWAQVADLGDRAVESGTQLYEVNAPELLPYIEGYAHTGQWEKS
                     RQLTLEAERLTFRMQRSLCDAWERILDATPDSEARRSAHEAVQSRLQCTGP"
BASE COUNT          691 a          868 c          932 g          528 t
ORIGIN      
        1 ttaattttac tgcgttcgat cgggcgttgg gacaggcatt ggagcgttgg ttgcgatggt
       61 ctgttcgctg attacataag ctgggcggtc ctgcaggcga aaatgcatgc gtgccaggta
      121 ttcacccatg atgcccaggg caaacagctg caccccggag aaaatggcaa ctgttgaagc
      181 cagaaaagga aagcccggca cgctgccgcc ctggatcaga tagcgaccga ccacatagac
      241 cagcacgacc aggccaaaga tggcaaacag aaatcccatc aggctggcga actgcagcgg
      301 caggatgctg aacccggtga ccatattcat tgcatgccgc acgagcttac ggaaggtgta
      361 attggattgc ccggcatggc ggggatggtg tgcaaccggc acagcggcaa aacgggccgc
      421 tccccaggtg agcaggacat cgatcgaaac cgaagggctg tggtattcag caaagccctg
      481 gcgcactgcg ctgcggaagg cgcgaaacgc actcacctga cgggcggtat cgacgcccat
      541 cgtgctctgc agggctaatt tggtaacctg ggaggccagg tcgcgccaca ggccgtgctg
      601 ctcctgggca ggcgtgccat agaccacatc ccagccgcca gccagcttat ccagcaggcg
      661 gggaatctca gccggtggat tctgcaggtc atcatccagg gtgacgatga cttcgccgcg
      721 cgcctggcga atcccgcaca gcaaagcatt gtgctgccca tagttgcgga gcagattgat
      781 gccgctcacc tgcgagtatc gctgggtcag ctgtgtgatt acttcccatg aattgtccgg
      841 gctgccatcg tttacgaaca cgatctcata atccgggacg cagccttcca gagtctggac
      901 taactgggca gccagctcgg cgatggtttc tgcaccccgg tagaccggga cgattacgga
      961 gcaggtgaag ggaaatttca tctttggatt ctcaggggcc ggtgcactgc aggcgggatt
     1021 ggacggcttc atgggctgag cggcgggctt cactatccgg ggtggcatcc aggatacgtt
     1081 cccaggcgtc acataggctt ctttgcatcc ggaaggtcag ccgttcggct tccagggtca
     1141 gctggcggga tttctcccac tgcccggtat gagcatagcc ttcaatatag ggcaggagct
     1201 cgggtgcatt gacctcgtaa agctgcgtgc ctgactctac tgcccggtca cccagatcag
     1261 caacctgtgc ccaatcccgg ttttgcagcg cgagctcggc tttttcataa taataacacc
     1321 agttgggggt gggttcgctg ccgaagtaag cctctacggg aaccgctggc cgggcagcct
     1381 ccgccaggat caggcccggg tcagagatat ccatcacgtc gccgatatat tttggttttt
     1441 gaggtacccg ggcatgccgg gcggggtcat aaacaaccag gcaaccagga ggctggtagg
     1501 taactaccag gacctgggag gtggtgcccg taaatgcggt cgcgcggtac acctgttcga
     1561 tcggcagccc ggcttccagc cctgccaggg tcaggcctaa ccgggcgtcg atattgtaaa
     1621 acatgtaatc catggcgcga gcggtatgct ccggagcata cagccagtgg aagggtgccg
     1681 ccaatgagtt atcggtatag tggacgaatg gcagctctga ggtcaggatt gtcgtaccgg
     1741 gctggatcgc cggggcccgc cagctccact gccagaagaa attgcgctgg gtgttccatt
     1801 cccggcggta cgtattggca tgctggaatt ggagcccgac cgccaggccg accaggacgc
     1861 tgagcaccag caggcgtggc acaggctggc ggaccagcag gccgatcacg ccggccagga
     1921 gcaggctggc gccgaacatc atcgccaggg tgaagcgatc ccagggaaaa tccaggccga
     1981 ttggatagcc ggcgccccag aagggccagc ccccgaccag caggccggcg atgcccagca
     2041 gtatggcatc tttagcccaa ttttggtgtg catatggttg gctggcgctg ccagcactgg
     2101 cagtgttcgc tgcttcatcc ccggtgagtc ccttcgtata ggcagacaag agcagggctg
     2161 cggcaattac gcttgccaga acgatcgcgg catagacaac aattgatgac aatccgaaat
     2221 tagccagcag cccgaactgc agagtttgcc cccaggcgag gacgctggag gaaaaaatat
     2281 cctgccccat cgattggagt aaacccgaca gggcgttcac cgggtccgac agcaggcgcg
     2341 ggatcagcct tacctcgccg cgcggtgtat ccgaaaggaa cacccgccat acgaagaacg
     2401 ctgccaggag cagcaggtag ggcgcccaga ccaccagaat tcgtttcagc cgtttcccgg
     2461 cctcagggat tttttcacgc agcacaaacc acaggatcag cgggcgcagc agctccaggc
     2521 cgaaaaaata ttccaccgta aacagcacca gtcctgagca agccagcgat accatcatac
     2581 agagccagaa acgctccggt cggcggacag accagaccat gctggtaagc gaaaacagga
     2641 agactgccag gacgataaat acctggctgt aggtgaccga gataaattgc tgcgaaaagc
     2701 cgggatagag gcaaaacagc agggctgtcc aggcagcagg ctgggggtgg cgcggccaga
     2761 tcgcgcgcag caggctccag aatgccagcc cgctgagcca gcgggtgagg atgccgaaaa
     2821 cctgccaggc tgccggcgtc tcgccgacca gtggggaagt gatccggaac aatactccca
     2881 acaggggccg gtcggacgcg aagacatctt tgaaaatatc tgcgccccag aaatgcaggt
     2941 accagatcgt aggccagtcg tcccagtaaa agcccagcca cggcagcagc aatccatagg
     3001 ctgccacaca gaacaggag
//
LOCUS       DATMXK010000189         2163 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_162034, whole genome shotgun sequence.
ACCESSION   DATMXK010000189 DATMXK010000000
VERSION     DATMXK010000189.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2163)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2163)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2163
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162034"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..844
                     /locus_tag="VN363_03555"
     CDS             <1..844
                     /locus_tag="VN363_03555"
                     /inference="COORDINATES: protein motif:HMM:NF014826.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PHP domain-containing protein"
                     /protein_id="HWQ83614.1"
                     /translation="HLHSYYSDGCISPAGLLHAAAEDGLKTVALTDHDTLAGLEEAGH
                     VADKLGLELISGVELTCSWFGTDSPQIVDLMGYFFDPANPALLAACQEGLVDLEKRML
                     LACKYLGELGMQLSYFDLQEQNPRYAGAGQLIAALVQSGQVLDWLASYHMFSRVWPRL
                     PLPKLSLENAIDQLHAAGGVAVLAHPVAVRGHNGWLQAVELAPLVAAGLDGLETEHPR
                     LDHAAKEHFRGLAARFNLALSGGSDEHGWNGGVTRLGSELVTYDMVHELHNRAAGTRS
                     EPAG"
     gene            924..1136
                     /locus_tag="VN363_03560"
     CDS             924..1136
                     /locus_tag="VN363_03560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83615.1"
                     /translation="MSPRSTPKSSRELASQITRAASQQTYLTIRLLADPGQADDAYRA
                     YAYFRWVDDWLDEPGRPAPAPGCPPR"
     gene            1183..2070
                     /locus_tag="VN363_03565"
     CDS             1183..2070
                     /locus_tag="VN363_03565"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ83616.1"
                     /translation="MTDLTGKTAVITGGTRGLGLVAAQEFIAAGANVVLSSRSVPAVQ
                     QALASLPAQHAAGTACDVADREQVQALADFALQRFGGFEIWINNAGLSAPYGPTAHVP
                     PDLVERVIHTNILGTYHGSVTALAHFLPRRQGKLINVLGRGFDKPVPNQNSYASSKAW
                     MRVFTLALAKEYRSCGVGIFAYNPGLMFTEMVGDVDVLAGYETHVGALETVLRMWGNP
                     PQIPARRMVWMASAATDGITGKVYSELTTGRLFRGALTEGLRILLRRDPGEKRLTIHS
                     VDPALPDIPLPGAPRQGTN"
BASE COUNT          416 a          656 c          701 g          390 t
ORIGIN      
        1 ccatttacat agttattatt cagatggctg tatctccccg gccggtctgc tccatgcggc
       61 tgctgaggac gggctgaaga ccgttgcgct gaccgaccac gacacgctgg cagggctgga
      121 ggaagccggg catgtggctg ataagctggg attggagctg atctccgggg tggagctgac
      181 ctgcagctgg tttgggacgg attcacccca gattgtcgac ttgatgggct atttcttcga
      241 tccggcgaac ccggccctgc tggcagcctg ccaggaggga ttggtcgacc tggaaaaacg
      301 gatgctgctg gcctgtaagt acctgggaga gctgggcatg cagctgagct atttcgacct
      361 gcaggagcag aatccgcgct atgctggagc cgggcagttg attgccgccc tggtccagag
      421 cgggcaggtg ctggattggc ttgcgagtta ccatatgttc agccgggtgt ggccgcgcct
      481 gcccctgccg aagctcagcc tggaaaatgc gattgaccag ctccacgccg cgggaggcgt
      541 ggcagtgctg gcgcatccgg tggcggtgcg cgggcataat ggctggctgc aggctgtgga
      601 gctggccccg ctggtcgccg ccgggctgga tggcctggag acagagcatc cccgcctgga
      661 tcatgccgca aaggaacact tccgggggct ggcagcccgc ttcaacctgg ccctgagcgg
      721 cggatcggat gaacacggct ggaatggagg ggtcactcgc ctgggcagcg agctggtgac
      781 ctatgatatg gtgcacgagt tacacaatcg cgctgccggt accagatccg aaccggcagg
      841 ttgaataaac cacggaactt aatggtccct gaaacgtata taaaaagatc actatcggta
      901 aatactggag cttcgccaaa taaatgtccc cgcgcagtac tccaaaatcc agccgtgagc
      961 ttgcctccca gatcacccgg gctgccagcc agcaaaccta tctgacgatc cggctgctgg
     1021 ctgaccccgg ccaggcagat gacgcttacc gggcatacgc ttattttcgc tgggtggacg
     1081 actggctcga cgagccgggc cggcccgccc cggcaccggg atgcccaccg cgctgatctc
     1141 tggccgccag gcggcgcagc gcataatgga tgactttgcc gaatgaccga tttgaccggt
     1201 aaaaccgcag tgattacggg cgggacgcgc ggcctggggc tggtcgctgc ccaggaattc
     1261 atcgccgcgg gtgcgaacgt ggtcctcagc tcgcgcagtg tgccggcggt ccagcaggcg
     1321 ctggcgagcc tacctgcaca gcacgccgcc ggcacagcct gtgacgtggc tgaccgggaa
     1381 caggtgcagg ccctggcgga tttcgctctc cagcgctttg gcggcttcga gatctggatc
     1441 aacaacgccg ggctttcggc gccctacgga cccaccgccc acgtccctcc cgacctggtc
     1501 gagcgggtga tccataccaa catcctggga acctaccacg gctcggttac cgccctggcg
     1561 catttcctgc cccgccgcca ggggaagctg atcaacgtgc tgggcagggg attcgacaaa
     1621 ccggtaccca accagaacag ctatgcctcc agcaaagcct ggatgcgcgt cttcaccctg
     1681 gcgctggcga aagaataccg cagttgcggg gtggggattt tcgcctacaa tcccgggttg
     1741 atgttcaccg agatggtggg ggatgtggac gtgctggcgg gctacgagac ccatgtcggg
     1801 gcgctggaga cggtgctgcg gatgtggggg aacccgcccc agatccccgc ccggcgcatg
     1861 gtatggatgg cttcggcggc gaccgatggc atcaccggga aggtgtactc ggaattaacc
     1921 accgggcgtc tcttcagggg agcgctcaca gaaggcctgc gcatcctgct gcgccgcgac
     1981 ccgggtgaaa aacgcctgac catccacagt gtggatccgg cgctgccgga tatccccctg
     2041 ccaggcgccc ccaggcaggg gacaaattaa cctgagaacc atcactccta ccggcgggta
     2101 accgcgaaag gaagggaacc atgccaaaaa taaataagct caatgccttg atcctggcat
     2161 ttc
//
LOCUS       DATMXK010000190         4425 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_79960, whole genome shotgun sequence.
ACCESSION   DATMXK010000190 DATMXK010000000
VERSION     DATMXK010000190.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4425)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4425)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4425
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79960"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(235..1668)
                     /gene="murD"
                     /locus_tag="VN363_03570"
     CDS             complement(235..1668)
                     /gene="murD"
                     /locus_tag="VN363_03570"
                     /EC_number="6.3.2.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR01087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008764 -
                     UDP-N-acetylmuramoylalanine-D-glutamate ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-L-alanine--D-glutamate
                     ligase"
                     /protein_id="HWQ83617.1"
                     /translation="MNQDWQAQRVVIIGAARQGLALAAYLLKHNARVTITDRRSPQEM
                     ESVRQQFMEQAGGQAEIEWVFGDHPLSLLDRTDWLAVSGGVPLTNPLVQAATERGIRL
                     TNDSQIFLEAAPCRVVGITGSAGKTTTTSLVGEIARAARQPGQVWVGGNIGKPLISSV
                     DEMKSADLAVMELSSFQLELMHTSPQIAAVLNITPNHLDRHVTMAAYTAAKTHILAHQ
                     TDQDIAVLGREDPGSRTLTEKVAGRLLTFGIEKPQEKGFSWEGTYLTARGELHLQTVH
                     PFADLHLFNREAILLRGEHNLMNVLAACAIAAAAELPIAAMQTAVGNFRGVPHRLEFV
                     RSWQGADWFNDSIATAPERSMAAIRAFEEPLILLAGGKDKDLPWDEFAMLVAQRVDHI
                     VVFGEAAEKIFSALQACNLHPDERGARPPLTISRCKDLRAAIHVAAELVEPGDVVLLS
                     PGGTSFDEFRDFEERGQCFSRWVKDLH"
     gene            complement(1665..2663)
                     /gene="mraY"
                     /locus_tag="VN363_03575"
     CDS             complement(1665..2663)
                     /gene="mraY"
                     /locus_tag="VN363_03575"
                     /EC_number="2.7.8.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008963 -
                     phospho-N-acetylmuramoyl-pentapeptide-transferase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phospho-N-acetylmuramoyl-pentapeptide-
                     transferase"
                     /protein_id="HWQ83618.1"
                     /translation="MTRYSLALALAGLSFLITVIWGEPLIRILRHFKIGKEIRVEGPE
                     RHFSKLGTPTMGGVMIVLPVVLITILMNASSVIGVTSVLGRSIMLPMLVMLGYAVLGA
                     IDDWEGMRGKRRGLGMRARTKFIIQWLLAIGTAWGLYYVLDVPNLYLPSFKNEIELSP
                     LFYIPIAAFIIVSTSNAVNLTDGLDGLAGLITSTALATYGGIALLQGQVFIARFCFTV
                     VGAIFGFLWFNVHPAMLFMGDTGSLALGATLGVVALMTGQWALLPVIAIIPLSTTLSD
                     ILQVGYFKLTGGKRLFKMAPLHHHFELSGWSETQIVQRFWLIGLIAALIGVALAVV"
     gene            complement(2666..4084)
                     /gene="murF"
                     /locus_tag="VN363_03580"
     CDS             complement(2666..4084)
                     /gene="murF"
                     /locus_tag="VN363_03580"
                     /EC_number="6.3.2.10"
                     /inference="COORDINATES: protein motif:HMM:TIGR01143.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0047480 -
                     UDP-N-acetylmuramoyl-tripeptide-D-alanyl-D-alanine ligase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D-
                     alanine ligase"
                     /protein_id="HWQ83619.1"
                     /translation="MLTFADIVEAICGTRPGQTHQVVTEAVIDSRKVNPGALFIALPG
                     EQVDGHDFVAEAFERGAVLALIQRDLPISLPVLDLRQECDLAYLNNLELPVCLRVDDN
                     VATLQQIARFWRMKLDVRVIGITGSVGKSTTKELVSEVLDQRYQTLRNPGNLNNEIGL
                     PLSILQLTPDHQRAVLEMGFYVPGEIALLCDIAKPQIGIVTNIGTVHAERAGTQEIIA
                     KGKAELVQALPPAPEGVAILNYDDTLVREMANQTRAQVFFYGLDAHADLWADSIEGLG
                     LDGIRFRLHFRNETIHMRVPLIGRHSVHTALRAAALGLVEGLTWQEIYNGLRSPHTQL
                     RMMTVRTQSGALILDDTYNASPESTLAALNLLDELAGKKIAVLGDMLELGQYERMGHE
                     MVGVRAAEVVDELVTVGVRGKFIAEAAVEAGLNGSAITSVDNCQQAIKVLENRLDNQT
                     TVLVKGSRGMQMDLIVSALEYR"
     gene            complement(4140..>4425)
                     /locus_tag="VN363_03585"
     CDS             complement(4140..>4425)
                     /locus_tag="VN363_03585"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWQ83620.1"
                     /translation="SSDALITGYRVAGKTGTAEIPTPYGYTSNLTNASFIGWGPVDDP
                     QFLVFIWLEKPQSAIWGSIVAAPVFKEVVEELVVLMDIPPDFVRLKLYGQ"
BASE COUNT         1087 a         1225 c         1128 g          985 t
ORIGIN      
        1 aatcaataaa agtaatcagg gttgcgatgc caattcccaa tactgcccac aagacctgac
       61 gctggaagat catggtaggc acgccgtaga tctggatcga gtcatcccag ctggcggaat
      121 gaaccatgat caagccaaag atgaccaggg agatcaacgt cagcattaat ggaacgtcaa
      181 tacccgctaa aactttcctg tttttagcgg ttggctgagc aggtgcatcc ggatttagtg
      241 taaatctttt acccaacgac tgaaacattg tccacgctcc tcaaaatccc ggaattcgtc
      301 aaaactggtt ccaccgggag aaagcagcac tacatcacca ggttcgacta attcggctgc
      361 gacatgaatg gctgcacgga gatctttgca gcggcttata gtgagtggcg gtcgcgcgcc
      421 acgctcgtca ggatgcaagt tgcaggcctg aagggcactg aaaatttttt ccgcagcctc
      481 accaaatacc actatgtgat ctacacgctg tgcgaccagc atcgcaaatt catcccaggg
      541 aagatccttg tctttaccgc cagccaagag gatcaatggc tcttcaaaag cacggattgc
      601 agccatactg cgctcgggtg ccgtcgcgat cgagtcgttg aaccaatcgg ctccttgcca
      661 gctgcgcaca aactccagcc ggtggggaac accccggaag ttccctaccg ccgtctgcat
      721 ggcagcgatt ggtagttcgg ccgctgctgc gattgcgcag gctgctaata cgttcattaa
      781 attatgctcg ccgcgcagca ggatcgcttc tcgattgaaa aggtgcagat cggcaaaagg
      841 atggacggtc tgcagatgaa gctcgccgcg agcggtgagg taagttcctt cccaggagaa
      901 tcccttttcc tgtggctttt caattccaaa ggtcaacagg cgcccggcga ctttttcggt
      961 cagtgttctg gaccctggat cttcacgccc caatacggca atatcctgat ccgtctggtg
     1021 agccaggatg tgagtcttgg cagcggtata agctgccatt gtgacatgcc ggtccagatg
     1081 gtttggagtg atatttagga cagccgcaat ttgaggtgag gtgtgcatca gctccaattg
     1141 aaaactactc aattccatga cagccagatc agccgatttc atttcatcca cactgctgat
     1201 caatggttta ccgatgttgc cgccgaccca gacctgaccc ggttgtctgg ctgcccgagc
     1261 gatttcaccc accaggctgg tggtagtcgt cttgccggca gagccggtga taccaacgac
     1321 cctgcaggga gctgcctcca ggaaaatttg cgagtcattg gtgaggcgga tgcctcgctc
     1381 ggtcgctgcc tggacgagtg gatttgtcag cggtactcca ccagaaacag ccagccaatc
     1441 tgttctatcc aacaggctca gtggatggtc gccgaatacc cactcgatct ccgcttgccc
     1501 tccggcttgc tccatgaact gctgccggac gctttccatt tcttgtggac tgcggcgatc
     1561 ggtgatcgtt acccgagcat tatgttttag caggtacgcc gcaagggcaa gcccttgccg
     1621 ggcagcccca ataatgacaa cacgctgagc ctgccagtcc tggttcatac gactgccagg
     1681 gcaacaccga tcaaggcggc gatcaggcca atcagccaga aacgttgcac aatctgtgtt
     1741 tcactccacc cggaaagctc gaaatggtgg tggagagggg ccattttaaa cagccgtttt
     1801 ccaccggtca gtttgaagta accgacctgg agaatatcgc tgagggttgt gctcaacggg
     1861 atgatcgcga tcacgggtaa gagcgcccac tgaccggtca tcagggcaac gacacccagc
     1921 gtagctccta aggccagcga tccagtgtcc cccataaata acatggctgg atggacattg
     1981 aaccacaaga aaccaaagat cgcgccgacc acggtgaagc aaaaccgggc tataaatacc
     2041 tgaccctgta aaagtgctat cccaccatat gttgctaaag cggttgatgt aatcagacct
     2101 gccagaccat ccaggccatc ggtgagattt accgcgttcg aggttgatac aatgatgaaa
     2161 gctgcgatcg gaatgtaaaa gagcgggctt aactcaatct cgttcttgaa actcggtaga
     2221 tataaattcg ggacatccag aacataatac aggccccagg ctgtaccgat cgccagcagc
     2281 cactggatga tgaatttggt ccgggcgcgc attcccaaac cacgccgctt accccgcatt
     2341 ccttcccaat catcaatcgc ccctaatacc gcataaccga gcataaccag cattggcagc
     2401 atgatcgaac gacccagtac ggatgtcacg ccgataacag agctggcatt catcaggatc
     2461 gtgatcagga caacgggtag gactatcatg accccgccca tggttggcgt ccccagtttg
     2521 gaaaagtgcc gctcggggcc ttcgactcgg atctccttgc caatcttaaa atggcgcagg
     2581 atccggatca agggctcccc ccagataacc gtgatcaaaa agctgaggcc agccaacgcc
     2641 agagccaggg agtatcgagt catggctagc ggtactccag ggcagataca atgaggtcca
     2701 tttgcatgcc gcgtgagcct ttgaccagga cggttgtctg gttatccagg cggttttcca
     2761 acactttgat tgcctgctgg caattatcta cgctggtaat tgcagagccg ttgaggccag
     2821 cttctacagc agcctcagca atgaatttcc cgcgcacccc tacggtgacc aattcatcca
     2881 caacttcagc cgcgcgcaca ccaaccatct catgtcccat gcgttcatac tgccccagtt
     2941 ccaacatgtc acccagcact gcgatcttct taccggctaa ttcatccagg agattcaagg
     3001 cagccagggt ggattcgggt gaagcattgt aagtgtcgtc caatatgagc gccccagatt
     3061 gggtccggac ggtcatcatg cgcagctggg tgtggggaga gcgcaatcca ttatagattt
     3121 cttgccatgt gagcccttca accagaccaa gcgcagcagc ccggagagcg gtatgcacgg
     3181 aatggcgccc gatcaggggg acacgcatat ggatcgtctc attgcgaaaa tgtaaacgga
     3241 aacgaatgcc atccagcccc agtccttcga tactatctgc ccacaaatct gcatgggcat
     3301 ccaggccgta gaaaaacact tgggcgcgag tctgatttgc catctcacgc accaaggtat
     3361 cgtcataatt taatattgcc acaccctctg gagcaggtgg cagcgcctgg acaagctcag
     3421 ctttcccttt ggcgatgatt tcctgggtgc cagcccgctc ggcatggact gtgccgatat
     3481 tggtgacgat cccgatttgg ggtttggcga tatcgcagag cagcgcaatc tcacccggaa
     3541 catagaaacc catctcgagc accgcacgtt ggtggtcggg agtcagctga aggatgctga
     3601 ggggtaagcc aatttcattg ttgagattcc ccgggttacg caaagtttga tatcgctgat
     3661 cgagcacttc cgagaccaat tctttcgttg tggatttgcc aacactgcct gtgattccaa
     3721 tcaccctgac atccagcttc atccgccaaa accgcgcaat ttgctgtaat gtggcaacat
     3781 tatcatccac ccgcaagcat acgggtagct ctaaattgtt aaggtacgcc aaatcacatt
     3841 cttgtcgtaa atcgagcacg ggcaggctga tgggaagatc acgttggatt aatgccaaca
     3901 ctgcaccgcg ttcgaatgcc tcagctacaa aatcatgccc gtcaacctgc tctcctggta
     3961 aagcaataaa cagcgctcct ggattgacct tgcgtgaatc aatcactgcc tcggttacta
     4021 cctgatgagt ctgtccgggg cgggtgccac atatggcttc tacgatatca gcaaaggtca
     4081 gcataccttt cgatgcagag ataaacctct atgatctgca atccttccgt cgatcagatt
     4141 tactgcccat agagcttgag gcgaacgaaa tcgggcggaa tatccatcag tacaaccagc
     4201 tcttcgacaa cttctttgaa gacgggagca gcgacgatcg agccccagat tgctgattgg
     4261 ggtttctcaa gccagatgaa aaccaggaat tgaggatcat caaccggacc ccatccgatg
     4321 aaggaagcat tggtcaggtt gctggtgtat ccgtaagggg tcgggatttc agccgttcca
     4381 gtttttccag ctacccggta gcctgtaatc agagcatctg aagac
//
LOCUS       DATMXK010000191         2684 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_2989, whole genome shotgun sequence.
ACCESSION   DATMXK010000191 DATMXK010000000
VERSION     DATMXK010000191.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2684)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2684)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2684
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2989"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..642)
                     /locus_tag="VN363_03590"
     CDS             complement(10..642)
                     /locus_tag="VN363_03590"
                     /inference="COORDINATES: protein motif:HMM:NF017984.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0019104 - DNA N-glycosylase activity
                     [Evidence IEA];
                     GO_process: GO:0036297 - interstrand cross-link repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="crosslink repair DNA glycosylase YcaQ family
                     protein"
                     /protein_id="HWQ83621.1"
                     /translation="MTDSPLIIDKITRRRFILGQQGLYPGRRYRGQAGAAEAMQAGAV
                     VQMDPLNVIARSHDLALFGRVLDYSPKILDNLQFSERQFFDYGGTVFVHPMDELPHWR
                     TVMRRKAQEPRRQKFVAEYQAVVAEVFQAITENGPLAGRMDARLQRAAGEFHLLGLWV
                     EPGEVVDEAYRTALAAGLRRLMRMVGAERLVCEAGVPPELAGELEERCND"
     gene            complement(705..1019)
                     /locus_tag="VN363_03595"
     CDS             complement(705..1019)
                     /locus_tag="VN363_03595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HigA family addiction module antitoxin"
                     /protein_id="HWQ83622.1"
                     /translation="MITEKLLPVHPGEVLLEEFIKPFGLSQNRLAIEIGVDARRINEI
                     VLGARSVSADTALRLGRYFGVSPQFWLGLQAEYDLDVARDNLGDRLEREVRPRPAPGP
                     AD"
     gene            complement(1031..1312)
                     /locus_tag="VN363_03600"
     CDS             complement(1031..1312)
                     /locus_tag="VN363_03600"
                     /inference="COORDINATES: protein motif:HMM:NF016875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II toxin-antitoxin system RelE/ParE family
                     toxin"
                     /protein_id="HWQ83623.1"
                     /translation="MIKTFKDIHTENVYHRWRSRRLPPDIQQVALRKLRMLNNATSLQ
                     DLRVPPANRLEKLSGVRVGQWSIRINDQWRICFEWRGSDAYNVEIVDYH"
     gene            complement(1380..2579)
                     /locus_tag="VN363_03605"
     CDS             complement(1380..2579)
                     /locus_tag="VN363_03605"
                     /inference="COORDINATES: protein motif:HMM:NF018805.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lipocalin-like domain-containing protein"
                     /protein_id="HWQ83624.1"
                     /translation="MTRRLYFLLALVLCASLSIFFLLRPSSTEPAPAAAAVSAPAVDP
                     ALYQGFARATGPMELFFPGDHGPHPDYQTEWWYYTGNLLTAEGRHFGYQLTFFRRALL
                     PAGAAPERQSDWGTRQAYMAHFALTDSAANEYHAFERLERGAAGLAGAQSEPFRAWLG
                     DWEVSETAPAVYRLHARAAAEDGESLAIDLELNDVRGPVLQGDRGYSQKGPDPGQASY
                     YYSLTRLESNGTVTVGGQAYPVSGLSWMDHEFSTSVLSPNQVGWDWLSLQVSLGAEQQ
                     PAELMLFQIRQADGSIDPFSSGTLVYPDGSTRTLKREDFTIAVTGTWKSPHSGAEYPA
                     GWRISAAEIGLELALTPVIPDQELNLSYSYWEGAVTIEGTLLGEAASGRGYVELTGYS
                     GSMSGEF"
BASE COUNT          535 a          837 c          772 g          540 t
ORIGIN      
        1 gactttaacc taatcattac agcgttcttc cagctcgccg gccagttcgg gcggtactcc
       61 tgcctcgcaa accagccgct ccgcaccaac catgcgcatc aagcggcgca gtccggctgc
      121 cagggcggtg cggtaggcct cgtctaccac ttcgcccggt tccacccaca gacccagcag
      181 gtggaattcg ccggcggcgc gctgcaggcg ggcgtccatg cggcctgcca gtggaccatt
      241 ttcagtaatt gcctggaaga cttctgctac cacagcctgg tactcagcca cgaacttctg
      301 gcggcgcggc tcctgagcct tgcgcctcat caccgtgcgc cagtggggca gttcgtccat
      361 gggatgcacg aaaaccgtgc cgccataatc gaagaactgg cgttcggaga actgcaaatt
      421 atccagaatt ttagggctgt aatccagcac ccgtccgaaa agagccagat catggctgcg
      481 agcgatgacg ttgagcgggt ccatctggac cacagccccg gcctgcatgg cctctgccgc
      541 ccccgcctgg ccgcggtagc gccgcccggg gtagagaccc tgctgcccca ggataaaacg
      601 ccgccgggtg attttgtcga tgattaaagg ggaatcagtc ataccaggct ccttttcggg
      661 gataatttta accgcgaatt actcccccgg agcctgtttt tgggttaatc cgccggacca
      721 ggtgccgggc gcggccgtac ttcgcgttcc aggcggtcgc ctagattatc gcgtgccacg
      781 tccagatcgt attccgcctg caagcccagc cagaattggg gagagacgcc aaaataacgc
      841 cccaggcgta aggccgtatc ggcgctcaca ctgcgcgcac ccagcacgat ctcgttaatc
      901 cgccgcgcat ccacgccgat ctcgatggcc agccggttct ggcttaatcc gaagggcttg
      961 atgaactctt cgagcaggac ttcgcccgga tgaacaggga gtaatttttc agtaatcatt
     1021 tttctcacca ttagtgatag tccacaattt ccacgttata agcatcgctt ccgcgccact
     1081 caaaacagat gcgccattga tcattaatcc ggatgctcca ttgacccaca cggacgccgc
     1141 tcaacttttc cagccggttg gcgggtggaa cccgtagatc ttgaagcgat gtggcgttgt
     1201 tgagcatacg cagcttccgc agagctactt gttgaatatc tggcggtagt ctgcgcgagc
     1261 gccaccggtg ataaacattt tcagtgtgga tgtctttgaa ggtttttatc acgctcttat
     1321 tatatagtgc gatgcacccg atgtcaagcg cctaaacgaa aatttaccgc gaattggatt
     1381 tagaattcgc cgctcatgga cccgctgtac ccggtcagct ccacgtaacc tcgcccgctg
     1441 gctgcctcgc ccaacagagt gccctcaatc gttaccgccc cttcccagta cgaatacgac
     1501 agatttaatt cctgatccgg tatcaccggg gtgagggcca gctccagacc aatctccgcg
     1561 gcgctgatcc gccagcccgc cgggtactcc gccccgctgt gggggctttt ccacgtaccg
     1621 gtaaccgcaa tcgtaaaatc ttcccgtttc agggtccggg tgctgccatc cgggtagacc
     1681 agcgtgccgc tggagaaggg atcgatgctc ccgtccgcct gccggatctg gaacagcatc
     1741 agctctgctg gctgctgctc tgcgcccagg ctcacctgca gcgagagcca gtcccagccc
     1801 acctggttgg ggctgagcac gctggtggag aattcgtggt ccatccagct cagcccgctc
     1861 accgggtaag cctgcccgcc aacagttacc gtcccgttcg attccaggcg cgtcaggctg
     1921 taataatacg acgcctgccc ggggtcggga cctttttgcg agtagccgcg gtcaccctgc
     1981 agcaccggac cgcgaacatc attcagctcc aggtcaatgg ccagggattc tccatcctcc
     2041 gccgcagccc gggcgtgcag ccggtaaacc gccggtgcgg tctcgcttac ctcccaatcc
     2101 cccagccagg cgcggaatgg ttcggattgc gctcctgcca acccggccgc gccgcgctcc
     2161 aggcgctcga aggcgtggta ttcatttgca gcactgtctg tcagcgcgaa atgggccata
     2221 tatgcctggc gtgtgcccca atccgactgg cgctccggag cagcgccggc cggcagcagg
     2281 gcgcggcgga aaaaggtgag ctggtagccg aagtgcctgc cttcagcagt cagcaggttg
     2341 ccggtgtaat accaccactc ggtctggtaa tcggggtgcg ggccgtggtc gccggggaag
     2401 aacagctcca tgggaccggt ggcgcgggcg aagccctgat atagagcggg atccactgcc
     2461 ggggcggaga ctgccgccgc agccggggct ggttcagtgg aagaggggcg gagcaggaag
     2521 aagatggaga ggctggcgca taaaacgagc gccagcagga agtaaagtcg ccgggtcatg
     2581 acgctaatat accttatttt cgtaggataa gctttagctt gtcttatgct ggcagacaaa
     2641 aagcctgaaa gtgacgcaga atcaccgcta tcaagtatag gctc
//
LOCUS       DATMXK010000192         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_69430, whole genome shotgun sequence.
ACCESSION   DATMXK010000192 DATMXK010000000
VERSION     DATMXK010000192.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_69430"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..1665)
                     /gene="guaB"
                     /locus_tag="VN363_03610"
     CDS             complement(205..1665)
                     /gene="guaB"
                     /locus_tag="VN363_03610"
                     /EC_number="1.1.1.205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559278.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003938 - IMP dehydrogenase activity
                     [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IMP dehydrogenase"
                     /protein_id="HWQ83625.1"
                     /translation="MKIRPNKGLTFDDVLLLPKRSPVTSRQDVDTSTQLVGDIRLKLP
                     IISANMDTVTEANMGVAMARAGGIGMIHRFMTIEAQAEQVRLVKRMYGYLIEKPYAIS
                     PEASIDQAEAAMRRWKVSGLVVIDNESRLLGLLTRRDLGISPKATVEVRQAMTPRERL
                     VTAGAQTTPEQARQLMYANRVEKLPVIDETDRLLGLITMRDLTKFERYPLATVDEKDR
                     LRVGAAVGVTENELKRAEALLEAGADVLVVDIAHGHADHCLNFVKRLKTTFPRALIVA
                     GNVATVEGARDLAQAGADAIKVGIGPGSICTTRIVTGFGVPQLTAIMDSREGLVKDGL
                     YLPIIADGGIRNSGDMVKALASGADSVMIGSLFAGCDEAPGVPVIRDGRKVKVVRGMA
                     SLGAAMGRRQVERGEDESAEDPEDWSKVVPEGVEAVVPYRGQVSEILYQLVGGLRSAL
                     SYGGACNIPELQENAEFVEITPAGMSESRSHDVDRV"
     gene            1952..>2244
                     /locus_tag="VN363_03615"
     CDS             1952..>2244
                     /locus_tag="VN363_03615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83626.1"
                     /translation="MESKSEPNTNPLAGMSQLRRLLQFVYPYRSRLYITLVAIVIGSV
                     LGLAAPYALQFLIDAVFKENDPDLLNRITLTLIGIFALQNVLYFIRGYLLAFIG"
BASE COUNT          473 a          698 c          529 g          544 t
ORIGIN      
        1 ggctggactg cggcaacgag catactcaat atcatggtga aagccagggc aaagtacgca
       61 acccgtgctt tcgaggttgt tttctgatta ctcattgttt tttatccttt ccgatatagc
      121 atacttaatc tcagcattag acgttcaagt gcggcgtcag gttcccggat tttgttttct
      181 ctttcaggca gaatatctat ccggctatac tcgatccacg tcatgcgagc ggctctcgct
      241 catccccgcc ggtgtgatct cgacgaattc ggcattctcc tgcagctcag ggatattgca
      301 cgccccgcca tagctcagcg ccgaacgcaa tccacctact aattggtata gaatttcgct
      361 cacctgaccc cggtaaggca ctaccgcctc caccccttcg ggcaccacct tggaccaatc
      421 ttccgggtct tcggcgcttt catcttcccc gcgctccacc tggcggcggc ccatcgccgc
      481 gcccagggag gccatcccgc gcacaacctt gaccttgcgc ccatcgcgga tcaccggcac
      541 acccggcgcc tcatcgcagc cggcaaacag gctcccgatc atgacactat ccgctccgga
      601 agccagcgct ttgaccatat cgcctgaatt gcggatcccg ccatcggcga tgatcggcaa
      661 atataggcca tccttcacca gcccttcccg gctgtccatg atcgcggtca gctgtgggac
      721 gccaaatccg gtgacaatcc gcgtggtgca gatcgagccg gggccgatgc caacctttat
      781 agcatcggcg cctgcctgag ccaggtcgcg ggctccctca acagtcgcca cattgcctgc
      841 tacaatcagg gcccgcggaa aggtcgtttt gagccttttt acgaagttca agcagtgatc
      901 tgcatgaccg tgggcgatat ccaccaccaa cacatccgca cctgcctcga gcagtgcctc
      961 cgctcttttc aattcatttt cggttacacc caccgccgcg ccgacccgca ggcggtcttt
     1021 ttcatctacc gtcgccagcg ggtaacgctc gaatttcgtt aaatcccgca tggtaatcag
     1081 ccccagcagg cgatcggtct catcaatgac cggtaatttc tccacccggt ttgcatacat
     1141 gagctggcga gcttgctcgg gtgtcgtctg tgccccggcc gtcaccagtc gttcgcgcgg
     1201 tgtcatcgcc tgacgcacct cgaccgtagc cttgggagag atacccagat ctcgccgggt
     1261 gagtaagcct aataaacggc tttcattatc gattactacc aggccgctta ccttccagcg
     1321 ccgcatggca gcctcagcct ggtcgatact cgcctccggg gaaattgcat agggtttctc
     1381 gataagataa ccgtacatgc gcttcaccag gcgcacctgc tccgcctgcg cttcgatggt
     1441 cataaagcgg tggatcattc cgatcccacc cgcccgcgcc attgctactc ccatattggc
     1501 ctctgtgacc gtatccatat tggcgctgat tatgggcagt ttcagccgga tatccccaac
     1561 caactgggtg gaggtatcca catcctggcg tgaagtcacc ggcgagcgct tggggagtaa
     1621 gagaacatcg tcaaacgtta aacctttatt ggggcggatt ttcatttaat tagctcctgg
     1681 gctaaacaac gcgaccataa ctatgattaa tatttgacca gaccagaaat ttttgaccat
     1741 atgcctgacc agaacgtggg tctttttcgc cggataagta tatcaggctc ctccgcagac
     1801 ttgatttctg attcattaaa atcactttct tggagccgga aatagcgctg ctcatccgaa
     1861 atccaaaagc ccctctcccg gttgaaatgg atgtgggaat taaccggatt ggcgcgcatt
     1921 aaatccacca atcatggttt aattatcagc tatggaatcc aaatcagaac ccaacaccaa
     1981 tcccctggct ggaatgtcac agctccgccg cttattacaa tttgtttacc cttaccgctc
     2041 ccgcctgtac ataaccctgg tggcaattgt catcggcagc gtgctgggcc tggcggcacc
     2101 gtacgcactg cagttcttga tcgatgccgt ctttaaggaa aacgacccgg atctacttaa
     2161 ccgcatcacc ctgacattaa tcggcatctt cgccctgcaa aatgtgctgt attttatccg
     2221 gggttacctg ctggctttta ttgg
//
LOCUS       DATMXK010000193         2250 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_46509, whole genome shotgun sequence.
ACCESSION   DATMXK010000193 DATMXK010000000
VERSION     DATMXK010000193.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2250)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2250)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2250
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46509"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..768)
                     /gene="larE"
                     /locus_tag="VN363_03620"
     CDS             complement(<1..768)
                     /gene="larE"
                     /locus_tag="VN363_03620"
                     /inference="COORDINATES: protein motif:HMM:TIGR00268.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWQ83627.1"
                     /translation="MELTLDVELAKRYQELKHVLHSLGRVLVAFSGGVDSALLAAAAY
                     EVLGSDALAVTAVSASLPGRELKDAQDLAQQIGIRHLLIETGELSDPRYANNPSDRCY
                     FCKDTLYTRLQALADAESIQWICNGANVDDLGDFRPGERAALEHRVRSPLREAGLNKI
                     HIRELARQMGLSAWDKPAMACLSSRVPFGLNITPEKLYQIEASENILHDLGYRQVRVR
                     HHGDLARIEVEPAERSRLLEQAEEISEQLRQFGFSFIT"
     gene            complement(1111..1338)
                     /locus_tag="VN363_03625"
     CDS             complement(1111..1338)
                     /locus_tag="VN363_03625"
                     /inference="COORDINATES: protein motif:HMM:NF013224.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Lrp/AsnC ligand binding domain-containing
                     protein"
                     /protein_id="HWQ83628.1"
                     /translation="MKAYILFKVRTGDIPEVLSHLKRLDGVSEANMTFGPYDIVAVVQ
                     TTDLQHLGHLLSQGIQPIPGVEGTLTCLAVD"
     gene            complement(1367..2215)
                     /locus_tag="VN363_03630"
     CDS             complement(1367..2215)
                     /locus_tag="VN363_03630"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561363.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008509 - monoatomic anion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006820 - monoatomic anion transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="formate/nitrite family transporter"
                     /protein_id="HWQ83629.1"
                     /translation="MAPDINIDALLPAEMARRAELLGVRKSEMKLSNMLGLAVLAGAF
                     IGLGAAFATTISAGTSGAWPYGVTRLLTGLVFCLGLILVIVGGAELFTGNNLIVMAWA
                     SRKVNTSALLRNWTIVYLGNFVGSIATALVIFFGKQYTFGGGAIGTSALAIANSKVHL
                     DFIQAIALGIMCNAMVCLAVWMSYSARSVTDKILAIIFPISGFVAAGFEHSIANMYFI
                     PIGLFIKQFDPAFAANSGLDLSGLTWGSFLVNNLLPVTIGNIIGGGGLVAIVYWYIFL
                     REAPPK"
BASE COUNT          571 a          587 c          572 g          520 t
ORIGIN      
        1 cgtgatgaag ctgaatccaa actggcgcag ttgttcactg atttcttcag cctgttcgag
       61 cagccggctc cgttcggctg gctctacttc aatccgggcc agatcaccgt ggtgccggac
      121 tcgcacctgg cgatagccta aatcatgcag gatattctca ctggcttcaa tctggtaaag
      181 cttttcgggg gtgatattca gtccaaacgg cacccgtgaa gacaggcatg ccatggcagg
      241 tttatcccag gcgctcaaac ccatctggcg ggcaagctcg cggatgtgga ttttattcaa
      301 tccagcttcc cgcagcggcg agcgcacccg gtgttccaga gcagcgcgct ccccgggtcg
      361 aaaatcaccc agatcatcca cgttggctcc attgcagatc cattgaatgc tctctgcgtc
      421 agcgagagcc tgcaaacgtg tatagagagt gtccttgcag aaataacacc ggtcagatgg
      481 attgttggcg tagcgcgggt cggataattc gccagtctca atcagaagat ggcggatacc
      541 gatttgctgg gctaagtcct gcgcatcttt gagttcacgc cccggcaagg aagccgacac
      601 agcagtcact gccagagcgt cactgcccaa aacctcatag gctgccgcag ccagcagggc
      661 ggaatcgaca ccgccggaaa atgccacgag cacgcgtccc agactgtgaa ggacgtgctt
      721 taactcctga taccttttgg ctaattcaac atccagggta agttccacgg ataacctcct
      781 ataccagtat atgttggata attataccta atctagcata gtgccctgac ctggatgttc
      841 aatgattcgg actttatccc catcaaggac ctagatgacg taccctggct tttgtccaaa
      901 agacagagtc tgtaattgta ataataagaa ccttcacccc acgatcattg gagtgggatt
      961 tctaagaagg aaagtgaatt cccgggagac tgcaatgggc tgacagacaa gagaaggcag
     1021 tggaaaagaa ataaaaacag ccgctcagat caggaatatt cattaaatac cccaaaaatc
     1081 ataagcggct ggttaaggat gttattaaac tcagtcaacc gccaggcagg tgagagttcc
     1141 ttcgactcct ggaataggtt ggatgccctg ggatagcaaa tgacccagat gctgcaggtc
     1201 cgttgtctgt acgaccgcca caatatcgta gggtccaaag gtcatattcg cttcgctgac
     1261 cccgtccaga cgtttcaaat ggctgaggac ttcaggtata tctccggtgc gaactttaaa
     1321 taaaatatat gctttcatcg tcgcgagctc cttggtggtc agtggactat ttgggtggtg
     1381 cctctctcag gaatatatac cagtagacaa tggctactaa tcctccaccc cctattatat
     1441 tgccaatcgt cacaggtagc aaattattca ccaggaaaga tccccaggtt aagccgctca
     1501 ggtcgagtcc cgagttagct gcaaatgctg gatcaaattg cttgatgaac aaaccgattg
     1561 ggatgaagta catattggcg atgctgtgtt cgaaccctgc cgccacaaag ccgctgatgg
     1621 ggaagatgat agcgagaatt ttgtcggtta cgctgcgggc gctgtatgac atccacactg
     1681 ccagacagac catggcgttg cacatgatcc caagggcaat agcctgaata aaatccaggt
     1741 ggactttact gttggcgata gccagggcgc tcgtcccaat tgcgccgccg ccaaaagtgt
     1801 attgtttgcc gaagaagatt accagggcag tggcaatgga acccacgaag ttgcccaggt
     1861 agacaatggt ccagttgcgg agaagagcgc tggtgttaac cttacggctg gcccatgcca
     1921 taacgatcag attgttgcct gtgaacagct ctgcgccgcc aacgattacc aggatcaggc
     1981 ccaggcagaa aaccagaccg gtgagcagcc gggtgacgcc gtagggccat gcaccactcg
     2041 tcccggctga aattgttgtt gcaaacgcgg ctccgagtcc tatgaaggcg cctgccagca
     2101 cagccagccc gagcatattg gacagtttca tttctgattt acgcacgccc agcagctctg
     2161 cgcgacgcgc catttcggct ggtaacaatg catcaatatt tatatctggt gccatttatt
     2221 tcagtcctcc gattttgtga tattttacac
//
LOCUS       DATMXK010000194         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_59192, whole genome shotgun sequence.
ACCESSION   DATMXK010000194 DATMXK010000000
VERSION     DATMXK010000194.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59192"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(289..855)
                     /gene="hpt"
                     /locus_tag="VN363_03635"
     CDS             complement(289..855)
                     /gene="hpt"
                     /locus_tag="VN363_03635"
                     /EC_number="2.4.2.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_006680773.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006166 - purine ribonucleoside salvage
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hypoxanthine phosphoribosyltransferase"
                     /protein_id="HWQ83630.1"
                     /translation="MNPNDYQSYAEIARDVREVLLTPEQIAQRISELGRQISMDYADK
                     DLLIVGVLKGVLMFMADLLRQITIPVAVDFIAVSSYSTEARDKGYVRLVKDLEIPITG
                     RNVLFIEDVIDTGLTLNYLLRNLRARSPASLEVCVMFDKPVRRLVEIPLRYRGFDIPD
                     QFVVGYGLDYREKYRNLPFVGLLKAEAL"
     gene            complement(852..1232)
                     /locus_tag="VN363_03640"
     CDS             complement(852..1232)
                     /locus_tag="VN363_03640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83631.1"
                     /translation="MSLPEKSRPVFAHPVEEEFARILDYYGIEWEYEPRTFPLEWDEQ
                     GNVTVAFTPDFYFTQQDLYVELTTLRPSLATFKNRKLRRMQELYPEIHIKLFKRREMR
                     NLMVKYGLQQEAARIKGTGAQEKP"
     gene            complement(1254..1847)
                     /locus_tag="VN363_03645"
     CDS             complement(1254..1847)
                     /locus_tag="VN363_03645"
                     /inference="COORDINATES: protein motif:HMM:NF024587.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidylate kinase-like family protein"
                     /protein_id="HWQ83632.1"
                     /translation="MPESDPIQRWSITLSRLMGSLGEEVALLAGEELGFRIVRKELIN
                     LAARQSGHPEIGLAAIDDLHLFNINPTPQATRTYQEALARLVHEEADRGACIIIGRAG
                     QAILKSAPRSLHVRITAPLELRVARVAARLGIPAEAAHEQIKATDRSRSYYVRRYYHI
                     RWDDPNEYDLVLNTGRLSASQAASMLCAALRARLQSI"
     gene            1941..3158
                     /gene="dinB"
                     /locus_tag="VN363_03650"
     CDS             1941..3158
                     /gene="dinB"
                     /locus_tag="VN363_03650"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF002677.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003684 - damaged DNA binding [Evidence
                     IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase IV"
                     /protein_id="HWQ83633.1"
                     /translation="MDDSKIGRAPRRIIHLDLDAFFCAVEELRSPELAGKPFAVGGQP
                     DQRGVVASCSYPARRFGVRSAMPMSRALRLCPELVIVSGRHSEYSAVSRQVMAILRRT
                     TQQLEQISIDEAFLDVSEHPDSLEILCHSLQGTIRSELGLPCSLGAASNKLVAKLATD
                     VGKARGRSAGAPADQPPCAITIVPAGEEAQFLAPLPVEMLWGVGPKTAERLKSLGIHT
                     IGELAGYNEIALAHKFGKNGYELVRHARGLDDRPVITFHEPKSISQEMTFARDVRSLP
                     ELLKTIDHQAEDVARSLQKQGASGSTVKIKLRWPDFTTLTRQSSLKTPTDQAAVISQA
                     ARELFLKEWREGKAVRLIGVGVSGLQGKARQLSLWDAPTERQQRLQTALHELEQRFGQ
                     EIFVHPERKKRSS"
BASE COUNT          681 a          881 c          979 g          620 t
ORIGIN      
        1 tttgggtcgg ggtaatttac ccttagtaac atgagcgtaa atcccgtatc gtaacccagc
       61 aggtggtttc cgggactacc tcaaggtcgt aaaccaataa tcagctcgat gagagttgtt
      121 gtggattatg gacagaggca agaacggaag gcatgatctc aagcacgctt gcagttcctt
      181 tctacaaagt aatatcgata gaaaggtaaa gcggaatgag aaatctttgg tggatcgaaa
      241 aaaggttact tgggacccct gcatttttat ttaagcagcg gcgggggatt agagcgcctc
      301 tgctttcagc agcccgacga acggcaaatt ccgatatttt tcacgataat ccaggccata
      361 gcccaccaca aactggtcgg ggatatcaaa gccgcgatag cgcagcggga tctcgaccag
      421 ccggcggacc ggtttgtcga acatcacgca cacctccagg ctggcagggc tgcgcgcccg
      481 caggttgcgg aggagataat tcagggtcag cccggtatcg atcacatcct cgatgaacag
      541 gacgttgcgc ccggtgatcg ggatttccag gtctttgacc aggcgaacat agcccttatc
      601 gcgggcttcc gtagaataac tggaaacggc gataaaatca accgcgaccg ggatcgtgat
      661 ctggcgcagc aggtctgcca tgaacatcag cacacccttc aagaccccca caatcagcaa
      721 atccttatct gcgtaatcca tggagatctg cctgcccagc tcagaaatgc gctgggcgat
      781 ctgttctggc gtaagcagga cctcgcgcac gtccctggcg atctcggcgt aagactggta
      841 atcattcggg ttcatggttt ttcctgagcg ccggtccctt tgatgcgcgc agcttcctgc
      901 tgtaagccgt acttgaccat cagattgcgc atttcgcggc gtttgaaaag cttgatatgg
      961 atctcggggt aaagctcctg catgcggcgg agcttgcggt ttttgaaggt cgccaggctg
     1021 gggcgcagtg tggtcagctc cacgtacagg tcctgctggg tgaaatagaa atctggcgtg
     1081 aaggctacgg tgacattgcc ctgttcgtcc cattcgaggg gaaaggtgcg cggttcgtat
     1141 tcccattcga tcccatagta atccaggatg cgggcaaatt cttcttcaac aggatgagcg
     1201 aataccgggc gggatttttc aggcagactc aatgcgaacc tcgacaggcc ggctcaaatg
     1261 gattggaggc gcgcccggag ggcagcgcac agcatactgg cagcctggga agcagacagg
     1321 cgtccggtgt tgagaacgag gtcgtactcg ttgggatcgt cccagcggat gtgataatag
     1381 cggcggacat aatagctccg gctgcggtcc gtggctttga tctgctcatg ggcggcttca
     1441 gcgggaatcc ccaggcgagc agcgacgcgg gcgacgcgca gctccagggg tgcagtgatc
     1501 cgcacatgaa ggctgcgcgg ggccgatttc aggatggcct gcccggcgcg gccgataatg
     1561 atgcacgccc cacgatcggc ttcttcatgt accagcctgg ccagggcttc ctggtaggtg
     1621 cgggttgcct gcggggtggg gttgatgttg aacaggtgca gatcgtcgat ggctgcaagg
     1681 ccgatttctg gatgaccgga ctggcgggcg gcgagattga tcaattcttt gcgcacgatg
     1741 cgaaagccga gctcctcacc ggcaagcaat gccacttctt cccccaggct gcccatcagg
     1801 cgggagaggg tgattgacca gcgctggatt ggatcggatt caggcatgcc ggacctccgt
     1861 ggttacccca tgcatcaaat aaattataca acagctttga aaattaaccg ccctgccagg
     1921 ggtcggggta aaatctggat atggacgatt ctaaaatcgg tcgtgcccca cggcgcatca
     1981 tccatctgga cctggatgcc ttcttctgtg cagtggaaga gctgcgcagc ccggaactgg
     2041 cgggcaagcc gttcgccgtg ggcggccagc cagaccagcg cggggtggtg gcgtcctgtt
     2101 cctatccggc gcggcgcttt ggagtgcgct cggccatgcc gatgagccgc gccctgcgcc
     2161 tgtgccccga gctggtgatc gtatccgggc ggcacagcga atattcggcg gtctcgcgcc
     2221 aggtgatggc gatcctgcgc cggaccaccc agcagctgga gcagatctcg atcgacgaag
     2281 ccttcctgga tgtgagcgag cacccggaca gcctggaaat tctatgtcac agcctgcagg
     2341 gaaccattcg cagcgagctg ggtttaccct gctccctggg agcagcgagc aataagctgg
     2401 tggcaaaact cgccaccgat gtcggcaaag cccgcggccg cagcgccgga gcacccgctg
     2461 accagccgcc ctgtgcaatt accattgtgc cggcgggtga agaggcccaa ttcctggcgc
     2521 cgctgcccgt agagatgctc tggggagtgg gaccaaagac cgctgagcgc ctgaaatcac
     2581 tgggaatcca caccatcggc gagctggcag gctacaacga aatcgccctg gcgcacaaat
     2641 tcgggaagaa tggctatgag ctggtccgcc acgctcgcgg cctggatgac cgcccggtaa
     2701 tcacctttca cgagccgaaa tcaatctcgc aggagatgac attcgcccgg gatgtgcgca
     2761 gcctgccgga gctgcttaaa accatcgacc accaggcgga ggatgttgcc aggagcctgc
     2821 agaagcaggg agcctcaggc agcacagtga agatcaagct gcgctggccc gattttacaa
     2881 ccctgacccg ccagagctcg ctcaaaacac ccaccgacca ggccgcagtg atcagccagg
     2941 cagcccgcga gctgtttttg aaggaatggc gggaagggaa ggctgtgcgc ctgatcggcg
     3001 tgggggtgag cggcctgcag gggaaagccc gccagctgtc tctgtgggat gcgcctactg
     3061 agcggcagca gcgcctgcag acggccctgc acgagctgga acagcgcttt gggcaggaga
     3121 ttttcgtcca tcctgagagg aaaaaacgat catcttagtg c
//
LOCUS       DATMXK010000195         2980 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118 k141_179,
            whole genome shotgun sequence.
ACCESSION   DATMXK010000195 DATMXK010000000
VERSION     DATMXK010000195.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2980)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2980)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2980
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_179"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..505)
                     /locus_tag="VN363_03655"
     CDS             complement(<1..505)
                     /locus_tag="VN363_03655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83634.1"
                     /translation="MISQRSLTLGLSAKFKDQYLGAVGYLAIFFFSITVVMITPTQRL
                     PWAAAVCLLAAALVYPQAFRSLVRLRWLVMIGLMALPPIFILGDLDRSLAGIPYSSEG
                     LLSGVQIGLRILVVLVAIQGMTSSVDISSVAGLLERAGLHGLGFSVGVALNLLPGLLQ
                     SAQNAWRS"
     gene            complement(502..966)
                     /locus_tag="VN363_03660"
     CDS             complement(502..966)
                     /locus_tag="VN363_03660"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ83635.1"
                     /translation="MKRLTVLILILLLASLTACAASNPPAAQEAAADTAPNLVISGGE
                     TSKTYSRADLESLPATQAAFKDVTYVGVTVSALLQDAGFDPAQIKAIKAVASDGYTMN
                     YDPGQLAGADIILAYARVDGDLAAEDGSFRLVLPGTEGKLNARMLVELKIIP"
     gene            complement(995..1603)
                     /locus_tag="VN363_03665"
     CDS             complement(995..1603)
                     /locus_tag="VN363_03665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83636.1"
                     /translation="MKLSTRELATLAVFGALWGLVEISLGAVLKTLNIPLSGVVLAAI
                     GLTIALVGRSFVPKRGSTLFIGVIAMLLKLFTLGGVILGPMVGILTEALVAEIVLSVA
                     GKPRRIIFVLAGALGVAWVLVQPFVTGPLLFGRTLFTVWLDLLDRGNQLLGLSPSAAV
                     WIVAALLAIHLAIGAVTGWLSWDIARLLQVRLGRPVTGTVKP"
     gene            complement(1584..>2980)
                     /locus_tag="VN363_03670"
     CDS             complement(1584..>2980)
                     /locus_tag="VN363_03670"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ83637.1"
                     /translation="VLVTGPSGCGKSTLGRVICGLIPHAIPAELTGEIQVAGLDILTQ
                     TIPTIAQKVGMVFQRPASQLFHLRAEDEVAFGPRNLGLGESEVQERTLWALQATGLID
                     LRHEKPSALSGGQKQCLAIAAVLAMRTEILVLDEPTASLDVPNTRRVMETLASLHAEY
                     GITSVLIEHRLAEAAQLAGRVILMEAGGIIAEGAPQEIFADKEIRMRLGLRRPTEEPS
                     VSWKTLIQSNGAQNSEHKPLLTLDNVSAGFNGRQVIQNIQLSFYPGEFAALVGDNGAG
                     KSTLAMVAAGLIKPQSGSVRFYNGVRPRPGLDVALLFQEPQEQLFTDSVDEEVAFGPH
                     NYDCFDLSQHIKILQEADLSEIRTRRPLALSVGQQQRTALAACLGLRPRLLILDEPTL
                     GQDWGHLQRLMNFLQALNQQGTTILLISHDYKLVHRYAERVILLRDGQIERIGKIQFS
                     TLNQTESIDETIHA"
BASE COUNT          656 a          864 c          891 g          569 t
ORIGIN      
        1 ggctgcgcca ggcattttga gcagactgaa gcaaaccggg cagtaagttg agggctactc
       61 ccacagaaaa acccagcccg tgcagcccgg cccgttccag cagcccggcg acggaagaga
      121 tatcgacgga gctggtcata ccctgaatgg cgaccagcac caccaggatg cgcaggccaa
      181 tctgtacccc gctgagcagg ccttcggatg aatacgggat cccggccagg ctgcgatcca
      241 gatccccaag gataaaaatt ggcggcaggg ccatcaggcc gatcatcacc agccagcgca
      301 ggcgcaccag gctgcgaaat gcctgcgggt agactaacgc tgcggccagc aaacagactg
      361 cggcagccca cggcaggcgc tgggtgggag tgatcatgac cactgtgatc gaaaagaaaa
      421 agatcgccag atagccaacc gcgccaaggt actggtcttt gaacttagcg gataagccca
      481 gggtcaggct gcgctgcgag atcatgggat gattttcagc tcgaccagca tccgtgcgtt
      541 cagcttgccc tcggtacccg gcagcaccag gcgaaatgag ccatcctccg cagccaggtc
      601 gccatccacg cgggcatagg caaggataat atccgcgcca gccagctggc ccgggtcgta
      661 attcatcgtg tacccatcac tggcaacagc cttgattgct ttgatctggg ctggatcgaa
      721 tccggcatcc tggagcagtg cggataccgt gacgcccacg taggtcacgt ctttgaaggc
      781 agcctgggta gccggcaggc tttccaggtc cgcccgggag taagttttgc tggtctctcc
      841 cccggatatc accaggttag gtgcagtatc ggcagcagcc tcctgggctg ctggtgggtt
      901 tgaggctgcg caagctgtta gcgatgcgag taagaggatc aggatgagaa cagtgaggcg
      961 tttcatttta tttatttctc caattatgat tctttcaggg tttgacagtg cctgtgaccg
     1021 gccgccccag acgcacctgc agcagccggg cgatatccca gctgagccaa ccggtaacgg
     1081 cgccgatggc tagatggatt gccagcaaag ccgcgacaat ccagactgcg gcggaggggc
     1141 tcagtcccag aagctggttg ccgcggtcga gcagatccag ccagacggtg aatagggtcc
     1201 gcccgaacag cagggggccg gtgacaaatg gctgcaccag cacccaggca actcccaggg
     1261 ctccggccag gacgaagatt atccggcgag gtttgcctgc cacgctgagg acgatctccg
     1321 cgaccagcgc ttcagtgagg attccaacca ttggcccgag gatcactccg cccagggtga
     1381 acagcttgag cagcatggcg atgaccccga taaacagggt agacccacgc ttggggacaa
     1441 aagacctgcc gaccagggcg atcgtcagac caatggcggc cagcaccact ccgctcaggg
     1501 ggatattcaa tgttttaagc actgccccca ggctgatctc aaccagcccc catagggctc
     1561 cgaaaacagc cagggtagcc agctcacgcg tggatagttt catcgatact ctccgtctgg
     1621 tttaaggtag aaaattggat ttttccgatt cgctcgatct gcccgtctcg cagcaggatg
     1681 acccgctcgg cgtaacgatg gaccagctta tagtcgtggg agatcagcag gatcgtagtg
     1741 ccttgctggt tgagcgcctg taaaaaattc atcaggcgct gcaggtgccc ccaatcctga
     1801 cccagggtgg gctcatccag gatcagcaaa cgcgggcgca gacccaggca ggcagccagg
     1861 gcggtgcgct gctgctggcc tacagatagg gccagcggtc ggcgggtgcg tatttccgaa
     1921 agatctgctt cctgaaggat ttttatgtgc tgggagaggt caaagcaatc atagttatgg
     1981 ggtccaaaag cgacttcttc atcgaccgaa tccgtaaaca gctgctcctg tggttcctgg
     2041 aagagcaggg ctacatccag cccgggacga ggccggactc cattgtaaaa acgcacactt
     2101 ccggattgag gtttgatcaa tccggctgcc accattgcca gggtagattt tccggctccg
     2161 ttatcgccaa ccagggctgc aaattcaccc ggatagaagc tcagctggat attctggatc
     2221 acctgccgtc cgttgaatcc ggcggaaaca ttatcgaggg tcaggagcgg cttatgctct
     2281 gaattctgag cgccattgga ttggatcaaa gttttccagg aaacggaagg ctcctcggtg
     2341 gggcggcgca gacccagacg catccggatc tctttatcgg caaagatctc ttgcggcgcg
     2401 ccttcggcga tgatcccacc agcttccatc aggatcaccc tgcctgccag ctgtgctgct
     2461 tccgccaggc ggtgttcgat caggacactg gtgatgccat attcagcatg cagggacgcc
     2521 agcgtttcca ttacccggcg agtattgggt acatccaggg aggcggttgg ctcatccagc
     2581 accaggatct cagtgcgcat ggccagcacg gcggcgatag ccaggcactg cttctgaccc
     2641 ccggaaagcg cagaaggctt ttcgtggcgc aaatcaatca ggccggtcgc ctgcaaggcc
     2701 cagagggttc gttcttgaac ttcggattcg cctaaaccca gattgcgggg tccaaaggcg
     2761 acttcatctt cggcgcgcag gtgaaagagc tgcgaggcag ggcgctgaaa gaccatcccg
     2821 actttttggg cgatagtggg gatcgtttgg gtgaggatat ccaggcccgc gacctggatt
     2881 tcccccgtca gctcagctgg gatggcatgc gggatcaagc cgcaaattac ccgccccagg
     2941 gtgcttttcc cacagccgga aggtccggta accagcacac
//
LOCUS       DATMXK010000196         2910 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_143641, whole genome shotgun sequence.
ACCESSION   DATMXK010000196 DATMXK010000000
VERSION     DATMXK010000196.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2910)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2910)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2910
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143641"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..443)
                     /locus_tag="VN363_03675"
     CDS             complement(<1..443)
                     /locus_tag="VN363_03675"
                     /EC_number="4.1.1.49"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004612 - phosphoenolpyruvate
                     carboxykinase (ATP) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006094 - gluconeogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HWQ83638.1"
                     /translation="MTTNKMGIASLYDLDRQGLRNLNMVYWTLPTPALVERIVTRREG
                     VLAHEGAVVVRTGIHTGRAPNDKFIVKTGAAEEAVAWGKLNRPMDPAHFDRLLIRMTA
                     YFQGRDVFVQDTTACAHPDYQLPIRVITENAWHSLFARNVFIRRS"
     gene            complement(614..2839)
                     /locus_tag="VN363_03680"
     CDS             complement(614..2839)
                     /locus_tag="VN363_03680"
                     /EC_number="2.7.7.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559481.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004654 - polyribonucleotide
                     nucleotidyltransferase activity [Evidence IEA];
                     GO_process: GO:0006396 - RNA processing [Evidence IEA];
                     GO_process: GO:0006402 - mRNA catabolic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyribonucleotide nucleotidyltransferase"
                     /protein_id="HWQ83639.1"
                     /translation="MEAQGKKFTAQIGGKEIVFETGRLAGQAGGAVTVQLGGTMVFAA
                     ATMSNDARPGIDYFPLTVDYEERMYAGGRIPGSFFRREGRPTEDAILTARLTDRPLRP
                     LFAQDMRNDVQVILYSFSADGENPIDILAVNAASTALMISDIPWGGPVAAVRVGYIGG
                     EFVLNPTFEETNTSQLDLRLAGTREAILMVECGADEVPEDLMVAALDFGHKALQPLID
                     VQLQMRDAVGKDKRSYTPYGIDPAILTAVKARSQAALVALLDRPYEKSGLYSGIDEIQ
                     SQVVAELTAEDETLVNSVKAAFDDALKDVVRQRILEKGIRPDGRGLTDIRPIWCDVNF
                     SPRAHGSGLFTRGETQVLTLATLGTPRDAQEMDNLTPVDSKRYIHHYNFPPFSTGEAR
                     PLRGTSRRDVGHGALAERALRPVIPAEADFPYTLRLVSEVLSSNGSSSMASVCGSTLA
                     LMDTGVPIKAPVSGVAMGLVKEGDRMAILTDILGMEDHLGDMDFKVAGTTRGITALQM
                     DIKIKGITAQLMTQALAQARIARLAILDKMMAVIPVPRPDLKPHVPRIITVKIPVDKI
                     GAIIGPGGKMIRSIQEETGAKIDVNEDGLVFIATSDGESARMARERIESLTDSAVIGR
                     IYTGKVVRIADFGAFVEILPNTDGLVHISQLDTERVESVEDVVRMGDEITVMVTDIDG
                     GGKIRLSRQAVLEGWTVEEAQSRDRQRSGGGRPGGGRPGGGRPGGDRRGGGDRDRGGR
                     R"
BASE COUNT          609 a          868 c          852 g          581 t
ORIGIN      
        1 aagctgcggc ggatgaacac attgcgggca aacaggctgt gccaggcgtt ttctgtaatc
       61 acccggatag gcagctgata atctggatgg gcgcaagcgg tagtatcctg gacgaataca
      121 tcccgcccct ggaagtaagc agtcatgcgg atcagcagcc ggtcgaagtg ggccgggtcc
      181 atggggcgat ttaacttacc ccaggcgacg gcttcctccg ccgcaccggt cttgacgatg
      241 aacttatcat tgggcgcacg cccggtgtga atacccgtgc ggacgacgac agccccttcg
      301 tgcgccagga ctccttcgcg ccgggtaacg atgcgctcga ccaacgccgg tgtgggcaaa
      361 gtccagtaga ccatattgag attgcgcaaa ccctggcggt ccaggtcata caggcttgct
      421 atccccattt tgttggttgt catcgacagc ctccctagat ttttagttcg ttctatggat
      481 aattttgacc ggaaaagtaa cccgactgct cagggcgtat tatacccaaa ggcgacaatt
      541 tcggtgagcc agtccaaaaa caggactgcc cataacggac agtcctgtcg tagcgaacga
      601 aagctgcggt ttattaccgg cgaccacccc ggtcgcggtc tccaccaccc cggcgatcac
      661 cgccgggacg cccgccaccg ggccgtccac ccccgggtcg accgccgccg ctgcgctggc
      721 gatcacgcga ctgggcttct tccacggtcc agccttccag gacagcctgg cgggagaggc
      781 ggatcttacc gccgccatcg atatcggtca ccattacagt gatctcatca cccatacgca
      841 cgacatcttc cacgctttct acccgttcgg tatccagctg ggagatatgc accaggccat
      901 cggtattggg caggatctcg acgaacgcgc caaaatcggc gatgcgcacg actttgccgg
      961 tataaatgcg cccgatcacg gccgaatcag tcagactttc gatgcgctca cgcgccatgc
     1021 gggcgctctc accatcgctg gtggcgatga aaacgagacc atcttcatta acatcgatct
     1081 tggcgccggt ttcttcctga atactgcgga tcatcttgcc accggggccg atgatcgcgc
     1141 cgatcttatc gaccgggatc ttcaccgtga taatgcgcgg tacgtggggc ttcaagtcgg
     1201 gccgcggtac agggatcact gccatcattt tgtccaggat cgccaggcgg gcaattcgag
     1261 cctgcgccag cgcctgggtc atcaattggg cggtgatgcc tttgatcttg atatccatct
     1321 gcagggcagt aatgccgcgg gtagtgccgg cgaccttgaa gtccatatcg cccaggtggt
     1381 cttccatgcc caggatatcg gtaaggattg ccatccggtc gccttctttg accagaccca
     1441 ttgccacccc ggaaacgggg gctttgatcg gtacaccggt gtccatcagc gccagggtcg
     1501 aaccgcacac cgaggccatc gatgaagagc cattcgaaga gagtacttca gaaaccaggc
     1561 gcagggtgta gggaaaatcg gcttcggctg ggatcaccgg gcgcagggcg cgctcggcca
     1621 gagcgccatg accgacatcg cggcgggaag tgccgcgcag cgggcgagct tcgccggttg
     1681 agaagggcgg gaagttgtag tggtggatgt agcgcttgga gtcaaccggg gtcaggttat
     1741 ccatttcctg ggcgtcacgg ggagttccca gagtcgccag ggtgagcacc tgagtctcgc
     1801 cgcgggtgaa cagaccagag ccatgcgcac gcgggctgaa gttgacgtcg caccagatgg
     1861 ggcggatatc ggtcaggccg cgcccatccg gacgaatgcc tttttccagg atgcgctggc
     1921 ggacgacatc tttgagggca tcatcaaatg cagctttgac gctgttgacc agggtctcat
     1981 cttcggcggt cagctcagcg acgacctgag attgaatctc atcgatgcca gaatacaggc
     2041 cggatttctc gtaagggcgg tccagcagcg ctaccagagc cgcctggctg cgcgctttga
     2101 ctgcagtcag gatggcaggg tcaatgccgt aaggtgtata gctgcgctta tctttaccga
     2161 cggcatcgcg catctgcagc tgaacatcga tcagcggctg caaagccttg tgaccgaagt
     2221 ccagagccgc gaccatcagg tcttcgggca cttcatcagc accgcattca accatcagga
     2281 tggcttcccg tgtcccggca aggcgcaaat ccagctgaga agtgttggtc tcctcgaagg
     2341 taggattgag aacgaactcg cctccgatgt acccaacgcg aacagccgcg actggcccac
     2401 cccagggaat atccgagatc atcaaagccg ttgaggcggc attgacagcc aggatatcga
     2461 tcgggttttc gccatccgcc gagaacgaat acagaatgac ctggacgtcg ttgcgcatgt
     2521 cctgcgcaaa cagcgggcgc agcgggcgat ccgtgagccg ggcggtgagg attgcatctt
     2581 cagtggggcg gccttcgcgg cggaaaaacg aacccgggat acgcccacct gcatacatgc
     2641 gctcttcata atcgactgtg agtggaaaat agtcgatgcc agggcgagca tcattgctca
     2701 tcgttgcagc agcgaacacc atcgtcccgc cgagctggac ggtcacagcg ccaccagcct
     2761 gcccggccag acgaccggtt tcaaatacaa tctctttccc gccgatctgg gcggtgaatt
     2821 tctttccttg tgcttccata tttctcctaa aaatcttgct cccgcctggt caggaactga
     2881 caattgctgc caaccagcgc agcatgaggc
//
LOCUS       DATMXK010000197        10985 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_71493, whole genome shotgun sequence.
ACCESSION   DATMXK010000197 DATMXK010000000
VERSION     DATMXK010000197.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10985)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10985)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10985
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71493"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            72..1388
                     /locus_tag="VN363_03685"
     CDS             72..1388
                     /locus_tag="VN363_03685"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HWQ83640.1"
                     /translation="MPKVAIVRTGALKGNAEFLPPTSKFVRYQEDIDQLKAKIEETIN
                     LAVGGIDNIIKPGETVLIKPNLAFQAPPESYDLVDPRVVEALIAFLKEKSQAKEVWIG
                     DNPSLGLHIGRARPSFETAGMRAAAERGGADRIIYFDEEELVEVDIPGALLFRKAKVF
                     KPFLDADRVINVPKMKNHLAGTVTLGLKNWNGIIPNEHPSGQQQDVHRIDLGQKFADL
                     YRLRRADLTLVDGIVAMEGQGPHRGTPVEMNLFTAGTDTVAVDAVTAYIMGYEPIEVA
                     AVRIAGTEGLGENDISKIEVVGTPLAEVRTFFKRTMLDPTGLIPGVRVIVQQTCPGCF
                     VNMRGSLDTFNVSGIDMKDYNEKVGDVYILAGGVPDFLPEWVEGKHLFVTGDCWKYFP
                     SHDKVEKAMTLAKSVTEYPGCAPVYIFAQLNGDLQALYNKALAGTD"
     gene            1578..2222
                     /gene="lipB"
                     /locus_tag="VN363_03690"
     CDS             1578..2222
                     /gene="lipB"
                     /locus_tag="VN363_03690"
                     /EC_number="2.3.1.181"
                     /inference="COORDINATES: protein motif:HMM:TIGR00214.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016979 - lipoate-protein ligase activity
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl(octanoyl) transferase LipB"
                     /protein_id="HWQ83641.1"
                     /translation="MVFKSRLENILPSVFILQENPPTFTIGHAGNSGNLLASAEELAR
                     RGIEMLEVSRGGDITYHGPGQIIASPLLYLGDIDLNANAYLHRLEDVLIELLAGYGIE
                     AGKQSDQPGVWVGDQKIGSVGIAVRHGYTFHGLSLNVNLDLSPFALINPCGVPQMQVT
                     SMQQLLNKPVPFDEVKALLKLGFAEIFGLEIEDQTWTNLQSKYGLSAEPTVKKE"
     gene            2227..2463
                     /locus_tag="VN363_03695"
     CDS             2227..2463
                     /locus_tag="VN363_03695"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWQ83642.1"
                     /translation="MQYPISVPRTADGALKVSVLRWMKSVGEDVIKGEDLAEVKTEKI
                     SLYVTAPESGKLTEILAQPGVVVPVGEVIGYVTG"
     gene            2450..3214
                     /locus_tag="VN363_03700"
     CDS             2450..3214
                     /locus_tag="VN363_03700"
                     /EC_number="1.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-oxoacyl-ACP reductase family protein"
                     /protein_id="HWQ83643.1"
                     /translation="MSLAKSLSLAGKVALVTGGGQGIGRTLCLALAEAGADIAVADIL
                     LPPAEQTAADIQAMGRKSLAIEADVGLGESVRNMVAACVEGLGRLDILVNNAGIFPIA
                     VVALMSEAEWDRVMDINLKGVFLCSQAALAPLRQSGSGRIINLASVSGLVGAVGMAAY
                     AASKAGVVGFTKSLAREVAPSAITVNAIAPGIILTEQAKHNFPPSSLQAYTGQVPLGR
                     LGTPDDLSAMVVFMASDAAAYVTGQVYAIDGGYTMQ"
     gene            3211..4029
                     /gene="larB"
                     /locus_tag="VN363_03705"
     CDS             3211..4029
                     /gene="larB"
                     /locus_tag="VN363_03705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0006189 - 'de novo' IMP biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarB"
                     /protein_id="HWQ83644.1"
                     /translation="MSSAEQRLTDLLEKVKNGSLPIEAALETLRTLPYEDIGYARLDH
                     HRALRQGFPEVVFAQGKTPDQVANIMLHLSERNDRVLATRASPEIYQIVHQHIPDAVY
                     HAMGRLIVINRATNSESAPGIVLASAGTADMPVAEEAAITAELMGNQVERLYDVGVAG
                     LHRLLDKAEVLRKAHVIIVIAGMEGALASVVAGLVSVPVIAVPTSVGYGASFGGLAPL
                     LTMLNSCAPGVAVVNIDNGYGAGHMASLINRQHTHAIQPTESHPPIIIQKGDDL"
     gene            4077..4886
                     /gene="larE"
                     /locus_tag="VN363_03710"
     CDS             4077..4886
                     /gene="larE"
                     /locus_tag="VN363_03710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560717.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent sacrificial sulfur transferase
                     LarE"
                     /protein_id="HWQ83645.1"
                     /translation="MHLQEILRQMGSVIIGFSGGVDSSLVAWVAHSVLEKQMLAVTVQ
                     SPVESPEETRLAIELAKRGGFRHMVVAMDDLADPVFSANPPDRCFHCKLRRFSALRAL
                     AQEQGIAWLADGTNADDAGDYRPGIRALQELGVRSPLAEAGFTKPEIRSLARALGLPN
                     WNKPAAPCLATRFPYDTRLTREEIERVGKAETYLHSLGLVGVRVRVHQDLARIETDPA
                     VFDALIAQRELIAAAFRQLGYLYVALDLLGYRTGSMNAPLADSFTKTNTTE"
     gene            4901..5812
                     /locus_tag="VN363_03715"
     CDS             4901..5812
                     /locus_tag="VN363_03715"
                     /inference="COORDINATES: protein motif:HMM:NF016777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ83646.1"
                     /translation="MADLIIDTHVHPKMGAAALLAEMDSAGVQRSVLLAVDTDPNDLR
                     DPQRLRETRLRFFKTPEARQVLWSEIETTMLQKLTPKVTNQIAADMVTAHPDRFIGLG
                     SINLSKDAAYVAAKLEEIERLGLQGIKLLPFSQFFDPAESENFRRVCDWSQKNGRVIL
                     IHTGCGAPPWDARAFSLEANPEKLRPALELYPEAHIIMAHMGSYSKDEPGIWFEEACK
                     LGAEFPNLYGDLAAVAWLVEREKRVVRMRETIGFKRILFASDYPAVANQVSIRYMIDL
                     VRNNPFLTEDEKLDILGRNAARLFEIA"
     gene            complement(5820..7340)
                     /locus_tag="VN363_03720"
     CDS             complement(5820..7340)
                     /locus_tag="VN363_03720"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560171.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YifB family Mg chelatase-like AAA ATPase"
                     /protein_id="HWQ83647.1"
                     /translation="MHSRVYSCAVVGLEGVIVDVEVDIGQGLPKMTIVGLPDTAVQES
                     RERVHAAIKNANLPYPRTPIVVNLAPATVRKEGPAYDLPIALGVLIMNHLVPQQAVNR
                     SLVIGELALDGKVRHTRGVLPMAAMARQEGFEQIFVPECDASEAALVPDLEVIPVNSL
                     EDLYLHLSGQKLIPPQPPTVQDAEIGGALTDFQEIKGQEHVKRALEVAAAGSHNVIMT
                     GSPGSGKTLMARALPSILPRISLDEALDVTRIYSVADQLPADVPLIRTRPFRSPHHTI
                     SHAGLVGGGNWPRPGEISLAHRGVLFLDELPEFGQRVLEVLRQPMEDKLVTISRAQGS
                     LSFPANFQLVAAMNPCPCGYYGDPVKPCTCAPGTITKYQKRISGPLLDRIDIHIEVPR
                     VDYEKLSDQRLGEPSAVIQARVEAARERQRLRFNGSDISCNADMRPAEVRQFCGLDET
                     GSALIRSAMRQLQLSARAYHRVLKLALTIADLAGCEKIQPQHLAEALQYRPRSLIN"
     gene            complement(7427..8974)
                     /locus_tag="VN363_03725"
     CDS             complement(7427..8974)
                     /locus_tag="VN363_03725"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWQ83648.1"
                     /translation="MALKSKHPSISGLESISPPRKLFRFAWQELAWHFLPPLVYFVIM
                     LGFSPYWDMFWIYSDEGFEVMKALLVSRGFSLFSQVWSDQPPLHTELLALIFRINGPG
                     VFAARLLTLCFTCLLIWALVQMVRLAWSNWAALAAAVFLLLLPAFPSLSVAALTGQPA
                     LALACVSLLGLFYWHRRPSWFVLVFSGSLMGLSLLTKAFTGILIPIFGGVFLFVEHRT
                     QPHPRNLWRTLWPALVWGSSILLSALGLSLLLVGPENLWQLIQPHVAAARSTAYPSNE
                     QLYSIWFYLRDAWTILLLAIAGIGIAFSQRRMWMAYPAVWMLAAMAVLSIYRPIWSHH
                     QLLVTIPAAMLAGGAAAEGVRWLAGQLKGGSILHRESILYGSTLVLALITLAVRWPDV
                     VSQFEVPGDMSAEPRSAYEDRVMRRINEYAPQTRWMVTDLPMYAFRAGLLTPPNLAVI
                     SWKRLAARDLTEAEILETIQELEPEQILFGRFRFDQVEDYIQEDYFPVLEREDQLKLY
                     IRKDLLK"
     gene            9122..10525
                     /gene="purB"
                     /locus_tag="VN363_03730"
     CDS             9122..10525
                     /gene="purB"
                     /locus_tag="VN363_03730"
                     /EC_number="4.3.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004018 - N6-(1,2-dicarboxyethyl)AMP
                     AMP-lyase (fumarate-forming) activity [Evidence IEA];
                     GO_process: GO:0009152 - purine ribonucleotide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate lyase"
                     /protein_id="HWQ83649.1"
                     /translation="MEINLETYQSPFTWRYGSAEMRQIWSEANKRRTWRKVWVALAEV
                     QSEYGLITPEQLADLRQAETRIDISRALEIEAEIQHDLMAELLTFTEQAPLGGSRLHL
                     GATSMDIEDNADVLRLGQALDIILAKLRQVLSALAEKVEVWAELPIMAFTHLQPAEPT
                     TLGYRMAGYAQDLWEDYELIARVRSNLRGKGFKGAVGTSAAYAELIGLDQLEPFEQRI
                     TEVLALPFYPITTQTYPRQQDYRVLSALAGLGGALYKFAFDLRLLQSPVLGELREPFG
                     SRQVGSSAMPFKRNPINAEKIDSLGRYLAQLPRIAWDNAAHSLLERTLDDSANRRILL
                     PEAFLAADEILRTAEQIIRNLEIDESAMQRNLAAYGPFAAVERVLMAYSKAGGDRQAA
                     HERLRRYAMTAWEAIQAGQSNPLRALIINDAEFSTVLGNHDLEVLMDASQYVGDAPRR
                     ARQMAARLRTDLQITPE"
     gene            complement(10533..>10985)
                     /locus_tag="VN363_03735"
     CDS             complement(10533..>10985)
                     /locus_tag="VN363_03735"
                     /EC_number="6.3.4.4"
                     /inference="COORDINATES: protein motif:HMM:NF012912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004019 - adenylosuccinate synthase
                     activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0006164 - purine nucleotide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylosuccinate synthetase"
                     /protein_id="HWQ83650.1"
                     /translation="ELTGGAGEVMLQKGQEYGTVTGRPRRCGWLDADLLRFTAQLNGF
                     SELVLTKLDVLDSLEKLQIGVGYRHPESGNELVHYWQGDARWLTRCDPVYEEISGWME
                     STAEARSFTALPQLAQAYIRWVEAFVGVRVSMVSVGPARSEVIPVPVL"
BASE COUNT         2570 a         3047 c         3017 g         2351 t
ORIGIN      
        1 ggaatatttc atcaatattt tggtcgaaat gttcaatgta tagttaacca atgtacgaaa
       61 ggagaaagat aatgccgaaa gtagcgattg ttcgtaccgg tgctttgaag ggtaatgcgg
      121 aattccttcc cccaacaagc aaatttgtcc gctatcagga agacatcgat caactcaaag
      181 cgaagattga ggaaacgatc aacctggctg ttggtgggat cgataacatc atcaagccgg
      241 gcgaaacggt tcttatcaag ccgaacctgg ccttccaggc gcctcccgaa agctatgatc
      301 ttgttgaccc acgagtggtc gaagcattga tcgctttctt gaaggaaaaa tcgcaagcga
      361 aagaagtctg gatcggcgat aacccatccc tgggcctaca tatcggacgc gcccgcccgt
      421 cttttgaaac cgccgggatg cgtgctgcag cagaacgcgg tggtgcggat cggatcatct
      481 acttcgatga ggaagagctg gtagaagttg atatccctgg cgccctgttg ttccgcaaag
      541 ccaaagtatt caaacccttc ctggatgccg atcgtgtcat caacgtgccc aagatgaaaa
      601 accacctggc tggcactgtg accctgggcc tgaaaaactg gaacgggatc atccccaatg
      661 agcaccccag tggtcagcag caagatgtcc accgcatcga cctcggtcaa aaatttgccg
      721 atctgtatcg cttacgcaga gccgacctga cccttgtaga tggcattgtt gccatggaag
      781 gccaggggcc gcaccgtggt acgccggtcg agatgaatct attcaccgct ggcaccgaca
      841 ctgtggcggt tgacgccgtt acggcctaca tcatgggtta tgagcccatc gaggttgctg
      901 ctgtccgcat cgccggaaca gaaggtctgg gcgagaatga tatcagcaaa attgaggttg
      961 tcggtacccc actggccgaa gtacgcacct ttttcaaacg caccatgctc gacccaaccg
     1021 gtttgatccc cggggtgcgc gtcatcgtcc agcaaacctg cccaggctgc ttcgtcaaca
     1081 tgcgtggctc tctggatacc ttcaacgtca gcggaatcga catgaaggat tacaatgaga
     1141 aagtgggcga tgtatacatc ctggcaggcg gcgtgccaga cttcctaccc gagtgggtcg
     1201 aaggcaaaca cctgttcgtt acgggagatt gttggaaata cttccccagc catgataagg
     1261 ttgaaaaagc catgaccctg gccaagagcg ttaccgaata tcctggctgt gcgccggtct
     1321 acatctttgc ccagttaaac ggcgacttgc aagcattgta taataaagcc ctggccggta
     1381 ccgattaatt taagtcgata ctcgctgccc gtagaatagg tttacaacct catggcgagg
     1441 gtgcaggtca ttcacccgat cttcaccctc gccagtgatt tatttcacgt ctaataaata
     1501 ggaggagagc cattctaaaa gcctactggc ttgatttagg ttatattccg tatccaccag
     1561 cttttgccat ccaggagatg gttttcaaat cgcggctgga aaatattctt ccaagtgttt
     1621 ttattcttca agagaaccct cctaccttca caatagggca tgccgggaac agcggaaacc
     1681 tattggcatc tgctgaggaa ttggctcggc ggggtatcga aatgctggaa gtcagtcgcg
     1741 gtggagacat cacctatcat ggacccggac aaatcattgc ctcgcccctt ttatatctgg
     1801 gcgatatcga tctcaatgcc aacgcctacc tgcatcgtct cgaagatgtt ctgattgagt
     1861 tgttggcagg ctatgggatc gaggcgggga agcagtctga tcagccggga gtgtgggttg
     1921 gagaccagaa gatcggctcg gtagggatcg ctgtccgcca tggatatacg ttccatggcc
     1981 tgtcattaaa tgtgaacctg gacttaagcc cttttgcact cattaatccc tgtggggttc
     2041 cgcagatgca ggtgacgtcc atgcagcagc ttttgaataa gcccgtacca tttgatgaag
     2101 tcaaagccct gctaaaacta ggatttgcgg agattttcgg attagaaatt gaagaccaga
     2161 cctggacaaa tctacaatca aaatatggac tttctgcaga accaaccgta aaaaaggagt
     2221 gatagcatgc aataccctat ctctgttcca cgtaccgcag atggcgccct caaagtatct
     2281 gttctgcgct ggatgaaatc cgtcggagaa gatgtgatta aaggcgaaga cctggcggaa
     2341 gtcaagacag aaaaaatttc cctgtatgtg actgcacccg aatcaggcaa actcaccgag
     2401 atcttggctc agccaggagt tgtggtgcct gtaggggagg taattggtta tgtcactggc
     2461 taagtccctg agtctggcag gaaaggtcgc tctggttacc ggaggcggtc aaggcattgg
     2521 ccgcacactc tgccttgcgc ttgccgaagc cggggctgat atcgctgttg ccgatatcct
     2581 gctcccgccc gccgagcaaa cagccgccga tatacaggcg atgggccgta aatccctggc
     2641 gatcgaagcc gacgtcggtc taggggaaag tgtgcgcaat atggtggctg cctgcgtgga
     2701 gggtctgggc aggctggata tcctggtcaa taatgccggg atctttccga tcgctgtggt
     2761 agctttgatg agcgaagctg aatgggaccg ggttatggac atcaacttga aaggcgtctt
     2821 tttgtgcagc caggctgctc tggcgccttt acgccagtcc ggcagcgggc ggatcatcaa
     2881 tctggcatcc gtctcaggtc tggttggcgc agtgggtatg gcggcttacg cggcttctaa
     2941 agcaggcgtg gtcggattca caaaatccct tgcccgggag gtcgctcctt cagccatcac
     3001 cgtgaacgcc atcgcaccag ggatcatcct cacggaacaa gccaaacata atttcccgcc
     3061 cagctccctc caggcctata cgggtcaggt tccgctcggt cgactgggca ctccggatga
     3121 tttgagcgcc atggtcgtct ttatggcttc agatgccgcc gcctatgtta ccggtcaagt
     3181 gtatgcgatc gatggagggt acacaatgca atgagcagtg cagagcagcg gttaaccgac
     3241 ttattggaaa aagtaaaaaa cggctcatta cctatcgaag ctgcgctgga aacacttcgc
     3301 acgttaccct acgaagacat agggtacgcc cgtctggatc accatcgcgc cttacgccag
     3361 ggtttcccgg aggtcgtttt tgcccaggga aaaactcccg accaggtagc gaatatcatg
     3421 ctgcacctct ctgagcgcaa cgatcgcgtc ctcgctacgc gtgcctcacc cgagatatac
     3481 cagattgttc accagcacat tccagatgcg gtctaccatg ctatgggccg cctgatcgtg
     3541 attaaccgcg ccaccaacag cgagagcgca ccggggattg ttctggccag cgcgggcaca
     3601 gccgatatgc cagtagcgga agaagccgcg attaccgccg aattgatggg caatcaggtc
     3661 gagcgcctgt atgacgtagg tgtggcaggc ctgcaccgct tgctggacaa agcggaggtc
     3721 ctccgcaagg cgcatgtgat catcgttatc gccggcatgg agggggctct ggcaagcgtc
     3781 gtcgccgggc tggtctctgt tccggttatt gccgtcccta ccagtgtcgg ttatggcgcc
     3841 agctttggcg gtctggcgcc gctgctcacg atgttaaata gctgcgcacc gggtgtcgcg
     3901 gtcgtcaata tcgacaatgg ctatggcgca ggtcatatgg ccagcctgat caatcgccaa
     3961 cacactcacg cgatccaacc aacagaaagc catccgccga tcattattca aaagggagac
     4021 gatttataaa cgccaaaatt ctctcgaccc tcgagcagtc gctggcggag aaatatattc
     4081 accttcagga aattctccgc cagatgggct cggtgattat cggtttctcg ggcggtgtgg
     4141 acagcagtct ggtagcctgg gttgctcatt ccgtgctgga aaaacagatg ctcgctgtga
     4201 ccgtgcaatc acccgtggaa tcaccggagg agacccggct ggcaattgag ctggcgaagc
     4261 ggggcgggtt ccgccatatg gtcgtcgcaa tggatgatct ggccgatccg gtattttctg
     4321 ccaacccacc cgatcgctgc ttccactgca agctgaggcg atttagcgct ttgcgtgctc
     4381 tggcccagga gcaaggaatt gcctggctgg cagatggcac gaacgccgac gatgccggcg
     4441 actaccggcc tggaatccgc gctctacaag aattgggggt acgcagtcct ctggctgagg
     4501 ctggattcac aaagcccgaa atacgttccc tggcccgggc attggggctt cccaactgga
     4561 ataaacctgc cgctccgtgt ctggcaacgc gtttccctta cgatacgcgc cttacccgtg
     4621 aggagatcga acgggtcgga aaagccgaaa cttacctgca cagcctcggg ctggttgggg
     4681 ttcgggtgcg cgtccatcag gatctggcac ggatcgagac tgatcctgcg gtttttgacg
     4741 ctctgattgc tcagcgagag ctgatcgcgg cagctttccg ccagcttggt tatctttatg
     4801 ttgcccttga cctgctcggc taccggacag gcagtatgaa tgcgcccctc gcggacagct
     4861 ttaccaaaac caacactacc gaatagggag gctagtaatc atggctgatc tgatcatcga
     4921 tacccatgtc catcccaaaa tgggggcagc tgccctgtta gccgagatgg atagtgccgg
     4981 ggtgcagcga tctgtgctgc tggcagttga tacagatcca aacgatctgc gtgaccctca
     5041 gcgcttacgc gagacccgac tgcgcttttt caagacacct gaagcgagac aggttctctg
     5101 gtctgaaatt gaaaccacga tgctccaaaa gctcacccct aaagtaacca accagatcgc
     5161 ggctgacatg gttacggccc accccgatcg cttcatcggc ctgggttcta tcaatctgag
     5221 taaagacgcc gcttatgtgg cagccaaatt agaggaaatt gagcgcctgg ggctgcaggg
     5281 gattaagctg ctgcccttct cccaattttt cgaccctgcc gaaagcgaaa atttccgccg
     5341 ggtttgtgat tggagccaaa aaaacggccg ggtaattttg atccacacgg gctgtggagc
     5401 accaccctgg gatgcacgcg cgttttcatt ggaagccaac ccggaaaagc tccgtccagc
     5461 tctggagttg tatcctgaag cgcatattat catggctcac atggggagtt acagcaaaga
     5521 tgagccgggt atctggtttg aggaagcctg taaactcggg gcagaattcc ccaatttgta
     5581 cggagattta gcggcagtcg cctggttggt tgaacgggaa aagcgcgtgg ttcggatgcg
     5641 cgagacaatc ggattcaagc gcattttatt tgcctcggat tatccggcag tggctaacca
     5701 ggtgagcatc cgctatatga ttgacctggt gcggaataac ccattcctga ctgaagacga
     5761 aaaactggat attctgggcc gcaatgctgc ccggctattt gaaatcgcat aaaactggtt
     5821 taattgatca gggaacgcgg ccggtactgt aaggcctctg ccaggtgctg cggctggatt
     5881 ttttcacacc ctgccagatc agcaatcgtg agcgccagct tgagcacccg gtggtaagcc
     5941 cgcgccgaga gctgcagctg gcgcatcgcg ctgcggatca atgcgctgcc agtctcatcc
     6001 aacccgcaga attggcgcac ttctgccgga cgcatatccg cattacagga gatatcactc
     6061 ccattgaagc gcagccgctg gcgctcacgc gccgcttcca ctctcgcctg gatcaccgcg
     6121 ctcggctccc ccaggcgctg gtcgctcagc ttctcataat ctactctggg tacttcaata
     6181 tggatatcga tccggtccag cagcggtccg gagatacgct tctggtactt ggtgatcgtc
     6241 cctggcgcgc aggtacatgg cttaactgga tcaccgtaat agccacaagg gcatggattc
     6301 atcgccgcca ccagctgaaa attcgccgga aaggataatg agccttgcgc ccggctgatg
     6361 gttaccagtt tatcttccat cggttggcgc agcacttcca ggacgcgctg accgaactcg
     6421 ggcagctcat ccagaaacaa taccccgcga tgcgcaaggc tgatctcacc tggacgcggc
     6481 cagtttccgc ctcccaccag cccggcatgt gagatcgtgt gatgtgggga gcggaagggc
     6541 cgggtgcgga tcaaaggtac gtctgccggc agctgatcag ccacggaata aatgcgcgtg
     6601 acatccaggg cttcatccag gctgatgcgc ggcaggatgc ttggtaaagc gcgggccatg
     6661 agagtcttcc ccgagcccgg cgaacccgtc ataattacgt tatgtgaccc tgcggcagca
     6721 acttcgaggg cgcgcttgac atgctcctgc cccttgatct cctggaaatc ggtcagagca
     6781 ccgccgatct ctgcatcctg gacagtcggg ggctggggag gaatgagttt ttgacctgaa
     6841 aggtgaagat acaaatcttc cagagaattg accgggatca cttcaagatc cggcaccagg
     6901 gcagcctcgg aggcatcgca ttccggcaca aatatctgtt cgaacccttc ctgacgcgcc
     6961 atcgctgcca tcggcaagac cccgcgggta tggcgaactt ttccatccag ggccagctcg
     7021 ccaattacca gggagcggtt aacagcctgc tgaggcacga ggtgattcat aatcagcaca
     7081 cccagagcga tgggcaggtc gtacgccgga ccttctttgc gtaccgtggc cggggccagg
     7141 ttaactacaa tcggtgtgcg tgggtaaggt agattggcat tcttgatcgc ggcgtgcacc
     7201 cgctcacggc tttcctgaac cgcggtgtca ggcagaccaa cgatggtcat tttcggcagt
     7261 ccctggccga tgtccacttc gacatcgacg ataaccccct ctaagccaac cacagcacac
     7321 gaatagacac gggaatgcat agggtaagtt tagatagagt gaggttaatt gtcaatgctc
     7381 gtaagcaggg ttggaccgga cagttctgta atgcccgggt gaagcatcat ttgagtaaat
     7441 ccttgcggat atacagcttg agctgatctt ctcgctccag gacagggaaa tagtcttcct
     7501 ggatataatc ttcaacctga tcaaatctaa accgcccaaa taagatctgt tctggctcca
     7561 gctcctggat agtctccagg atttccgctt cagtgagatc cctggctgcc aaccgcttcc
     7621 aggagatgac tgccaggttc ggtggggtta ataagccagc tcggaacgca tacatgggca
     7681 ggtcagtaac catccagcgc gtttgaggtg catattcatt tatccgccgc atcacccggt
     7741 cctcgtaggc tgagcgcggc tcagcggaca tatcgcctgg cacttcaaat tggctgacca
     7801 catcaggcca gcgtaccgcc agtgtaatca gcgccaacac gagcgtcgaa ccatataata
     7861 tactttcccg atgaagaatt gatccccctt ttaactgccc cgccagccat cgcacaccct
     7921 cagcggctgc cccgccagcc agcatggctg ctggaatggt aaccagcagc tggtgatggg
     7981 accagattgg acggtaaata gagaggacag ccatcgctgc caacatccag actgctggat
     8041 aagccatcca catccgccgt tgggaaaacg caatcccgat ccctgcaatc gccagcagta
     8101 gaatcgtcca ggcatctcgc aaataaaacc agattgaata aagctgctca ttggaggggt
     8161 aagcagtaga tcgggctgct gcaacatggg gctggatgag ctgccacaga ttctctggtc
     8221 cgaccaggag cagacttaac cccaaagcac ttaataaaat gctggagccc cacaccagag
     8281 ctggccacaa tgtcctccat aaatttctgg gatgtggctg ggtacgatgc tccacaaaca
     8341 ggaacacccc gccgaatatc ggaatcagaa tcccggtgaa cgctttggtg agcaaagata
     8401 gccccatcag gcttcccgag aagaccagga caaaccacga cgggcggcga tgccagtaga
     8461 ataaacccag taaggatacg cacgccagcg ccagggcagg ctgtccagtt aatgctgcca
     8521 cgctgaggga tggaaatgct ggcagtaaca gcaggaagac tgccgcagcc agagctgccc
     8581 aattactcca ggctaaccgg accatctgca caagtgccca aatgagcagg caggtaaaac
     8641 acaatgtcag caaacgagct gcgaaaacac caggcccatt aatgcgaaat atcaaagcca
     8701 gcagctccgt atgcaagggc ggttgatcgc tccacacctg gctaaaaagc gagaagcctc
     8761 tggaaaccag caaagctttc ataacttcaa agccttcatc ggaatatatc cagaacatat
     8821 cccaataggg agaaaagccc agcatgatca cgaaatagac cagaggcgga agaaaatgcc
     8881 aggcgagctc ttgccaggcg aatcgaaaaa gctttctcgg gggagagatt gattcgagac
     8941 cggagatgga tggatgcttg ctttttaatg ccataatcgc ttagacctag tttagggtga
     9001 tcctgaacgg atgtcaatgc tcaagatgat ggggatgctt ctcttatcag aggatcgggg
     9061 tgtggtaaga taatttcagg caagttcatc cccaagctgt tccattcaac tgcaggccgg
     9121 catggaaatc aacctcgaga cttaccaatc cccttttacc tggcgttatg gcagcgcgga
     9181 aatgcgccag atctggagcg aggctaacaa gcggcgtacc tggcggaaag tgtgggtggc
     9241 gcttgccgaa gtccagagtg aatatgggct gattaccccc gaacagctgg ccgatttacg
     9301 ccaggcagaa accagaatcg acatttccag ggcgctggag atcgaagccg agatccagca
     9361 tgatctgatg gcggagctgc ttactttcac agagcaagcg cccctgggcg gcagccgcct
     9421 gcacctgggc gccacctcca tggacatcga agataatgcc gatgtccttc gtctgggcca
     9481 ggcgctggat ataatcctgg cgaagctacg ccaggtatta tcggcactgg cagaaaaggt
     9541 cgaggtctgg gcagagctgc caattatggc cttcacccac ctgcagccag ctgagccgac
     9601 caccctcggc taccggatgg ctggatatgc ccaggatctg tgggaagatt acgagctgat
     9661 tgcccgggtg cgcagcaact tgcgcggtaa gggtttcaag ggtgccgtcg gcacctcggc
     9721 cgcttatgcc gagctaatcg gtttggatca attggaaccc ttcgaacagc gtatcacaga
     9781 agttctggcc ctgccgtttt atcctatcac cacccagacc tatccccgcc aacaagatta
     9841 tcgcgtgtta tctgccttgg caggcctggg gggcgcgctc tacaaattcg ccttcgatct
     9901 acgcctgctg caatcgccgg tcctgggaga actgcgtgag cctttcggct ccagacaggt
     9961 ggggtcgtcc gccatgccct tcaaacgcaa cccgatcaac gcagaaaaaa tcgactcgct
    10021 gggtcgttac ctggcgcagc tgccgcggat cgcctgggat aatgctgctc actcgctgct
    10081 ggagcgcaca ctggatgatt ctgccaaccg gcgcatcctg ctcccggagg cttttctggc
    10141 agccgacgag atactgcgca cggctgaaca gatcattcgg aatctggaaa ttgatgagtc
    10201 cgcgatgcag cgcaacctgg ctgcgtacgg cccttttgca gcagtcgagc gcgtcctgat
    10261 ggcttacagc aaagccgggg gagaccggca agctgctcat gaacgtttac gtcgatatgc
    10321 catgactgcc tgggaggcta ttcaggcggg tcagtctaat cccttgagag ccctgatcat
    10381 taacgatgca gaattttcaa ctgttctggg gaaccacgat cttgaagtat tgatggatgc
    10441 cagccagtat gttggcgatg caccccggcg agcacggcag atggcggcca gattacgcac
    10501 cgatctccag atcacacctg aatagaagcc ggttagagca ccggaacagg aatgacttcg
    10561 ctgcgcgccg gtcctaccga gaccatactg acccgtacgc ccacgaaagc ctccacccac
    10621 cggatatacg cctgggctaa ctgcggcagc gcggtaaagc tgcgcgcttc ggcagtggac
    10681 tccatccagc ctgatatttc ttcataaacc ggatcgcatc gcgtcagcca gcgcgcatcc
    10741 ccctgccagt aatgcaccag ctcatttccc gactccgggt gacgataccc gaccccgatc
    10801 tgcagctttt cgaggctgtc cagcacgtcc agcttggtca ggaccagctc gctgaagccg
    10861 ttaagctggg cagtgaagcg cagcaaatca gcatccagcc agccacagcg ccgcggccgc
    10921 ccggtcaccg tcccgtattc ctgccccttt tgcagcatca cctcgccggc gcctccagtt
    10981 agctc
//
LOCUS       DATMXK010000198         2439 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_14507, whole genome shotgun sequence.
ACCESSION   DATMXK010000198 DATMXK010000000
VERSION     DATMXK010000198.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2439)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2439)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2439
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14507"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..214
                     /locus_tag="VN363_03740"
     CDS             <1..214
                     /locus_tag="VN363_03740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83651.1"
                     /translation="FGAGRKTRDKLERMPLAITLATLTLVSAQPLGLHIQKQYTTSGK
                     PGNLEVVSITVKQRGEFKAYRITTQG"
     gene            222..1217
                     /gene="holA"
                     /locus_tag="VN363_03745"
     CDS             222..1217
                     /gene="holA"
                     /locus_tag="VN363_03745"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01128.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit delta"
                     /protein_id="HWQ83652.1"
                     /translation="MPPTIYILHGDDELAITRYLAEMEGRLGDPGTIALNTSRLDGNT
                     YNLNELIRVTSAMPFLAKRRLVILESFVGRLKDKPDQEAFQAVLLNVPPSTALVLVER
                     KPLVDERERRKNKEHWLEKWAKGAGDKALLKEFTAPKGAQLARWIQEEARAAGGEFTQ
                     KAAELLGQLVDDQPRQAAQEIHKLLAYVNYSRPVEPDDVHNLTTDSRQGNIFAMVDAL
                     SAKDGRLALQTLHRLLEEDAPAQIFPMIIRQFRLLILAREILDDVGPRANFPQLMGVP
                     QFVADKSVRQASQFSLPVLEAVYRRLLEIDEAVKSSQVEGDIALDTLVASFTSQK"
     gene            complement(1220..1471)
                     /locus_tag="VN363_03750"
     CDS             complement(1220..1471)
                     /locus_tag="VN363_03750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83653.1"
                     /translation="MMHVGMLWFDNDNKSGLNNRVERAAEYYQHKYGKTPNLCYVHPS
                     MLAPAEEKTDSALNTHGVQVRPLSTVLPNHFWIGISAES"
     gene            complement(1483..2394)
                     /locus_tag="VN363_03755"
     CDS             complement(1483..2394)
                     /locus_tag="VN363_03755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83654.1"
                     /translation="MITPDERLVLGLKALDRLRPALDKARLNIQTTLRPAQNRPRAVA
                     TAPSQPADPVVAKPPEIDSQTVPSLTDLLKQFGSLPDNAILLGICDDRMPFVLDLTNP
                     APGSILIAGDRHPDSLRLLDAMLVSAGLLNKASKVSYDLLCEAGNVSLQACSLANCNQ
                     VFDISDASAAERIVTLATLAEQRRRTTPRGPVLILAIEDLAALARQLNESDFARLFWL
                     VKHGPKARVWVIANLYAEDAELIDERLLGAFRTRLIGTTADPLRAAYLSGDEQVEAAS
                     LEPGRQYGVPFGEDWLTVWVCEPENLE"
BASE COUNT          551 a          646 c          749 g          493 t
ORIGIN      
        1 gtttggagca gggcgcaaaa cgcgcgacaa attggaacgg atgccgctgg cgatcacgct
       61 ggcgacgctg acactggtat ccgcccaacc cctgggcctg catatccaga aacaatatac
      121 gacttccggc aagccgggca atctggaggt ggtctcaatc accgtaaagc agcgcgggga
      181 gtttaaggct taccggatta cgacccaggg ctgaaaaggc gatgccgcca acgatctata
      241 tcctgcatgg ggatgatgag ctggcgatta cgcgctatct ggccgaaatg gaagggcggt
      301 tgggcgatcc tggcacgatt gctttgaata cttcccggct ggatggcaac acctataatc
      361 tgaatgagct gatccgggtg acctcggcaa tgccattcct ggcgaagagg cggctggtca
      421 tcctggagag ctttgtgggg cgactgaaag acaagcctga ccaggaggct ttccaggccg
      481 tattgttaaa tgtgccgcct tcgactgccc tggtgctggt ggagcgcaag ccgctggtag
      541 atgagagaga gcggcgcaaa aacaaagagc actggctgga gaagtgggcc aagggggcgg
      601 gtgataaagc gctgctgaaa gaatttacag cccccaaagg cgcccagctg gcgcgctgga
      661 tccaggagga ggcccgggca gcagggggag agtttaccca gaaggcagcg gaattacttg
      721 gccagctggt ggatgatcag ccccgccagg ctgcgcagga aattcacaag ctgctggcgt
      781 atgtgaatta cagccggccg gtggaaccgg atgatgtcca taacctgacg accgattcga
      841 ggcagggtaa catcttcgcc atggtcgatg cgctgtcggc aaaggatggg agattggcgc
      901 tgcaaacgct gcaccgttta ctggaagaag atgctccggc gcagatcttt ccaatgatca
      961 tccggcagtt ccgcctgctg atattggctc gagagatcct ggatgatgtg ggaccgcggg
     1021 cgaattttcc ccagctgatg ggtgtgcccc aatttgtggc tgataaatca gtccggcagg
     1081 cgtctcaatt cagcctgccg gtactggaag cggtttaccg ccgtctattg gagattgatg
     1141 aagcggtaaa atccagccag gtggagggcg atatcgccct ggatacactg gtggcatcgt
     1201 ttacctccca gaaataggat caagattctg cgctgatccc aatccagaag tgattgggca
     1261 gcaccgtgga gagcggccga acctgaacgc catgggtgtt cagggctgaa tccgtcttct
     1321 cctcagccgg ggcaagcata ctggggtgga cataacaaag attgggggtc ttgccatatt
     1381 tgtgctgata atattcagcg gcgcgctcga cccggttgtt caggccgctc ttgttatcat
     1441 tatcaaacca taacattcct acgtgcatca tcgcaaacac tcctactcca ggttttctgg
     1501 ctcacaaacc catactgtca gccaatcttc tccaaaaggc actccgtact gccgcccggg
     1561 ttccaggctg gcagcttcaa cctgctcatc gcccgacaga taagctgccc gaagcggatc
     1621 ggctgtggtg ccaatcagac gggtccgaaa tgcgcctaac aggcgctcat cgatcagctc
     1681 ggcgtcttcg gcatacagat tggcgatcac ccagacgcga gccttcggac catgctttac
     1741 cagccagaag aggcgggcaa aatcgctttc attgagctgt ctggccaggg cagccagatc
     1801 ctcgatcgcc aggatcaata ccgggccacg cggggtggtg cggcggcgct gctcggcaag
     1861 ggtcgccagg gtaacgatgc gttcggcagc cgaggcgtca ctgatatcga acacctggtt
     1921 gcagtttgcc agggagcaag cctgtaatga gacattgcca gcctcgcaga gcaagtcata
     1981 gcttactttg ctggccttat tcagcagccc tgcagagacc agcatcgcat cgaggagcct
     2041 gaggctatcc gggtggcgat cgccggcaat caagatcgac ccgggagccg gattggtcag
     2101 gtccaggacg aacggcatcc ggtcgtcgca aattcccaac aatatggcat tgtcgggcaa
     2161 gctgccgaac tgcttgagca gatcggtcag gctggggaca gtctgggagt caatctccgg
     2221 gggtttagca acaaccgggt ccgcaggctg agatggagca gttgcgactg cacgcgggcg
     2281 gttttgggcg ggccgcagtg tggtttgaat attcaggcgg gctttgtcca gggccggccg
     2341 aaggcgatca agggctttca aaccgagaac caggcgctca tccggggtga tcatagaagc
     2401 ctccatttga taaaacagaa cttatgttct attttatac
//
LOCUS       DATMXK010000199         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_80384, whole genome shotgun sequence.
ACCESSION   DATMXK010000199 DATMXK010000000
VERSION     DATMXK010000199.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80384"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1850)
                     /locus_tag="VN363_03760"
     CDS             complement(<1..1850)
                     /locus_tag="VN363_03760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83655.1"
                     /translation="MSQPKPLLRLVLLGLITLGCIFAWTIWQARTPTFGQSLTPSADI
                     PNSTISLFSTPAITQTISANEETATPTSTPTLVLKQPETWIETFGVLQDSLAVLALDE
                     NSHSQLFAYQPGLSPFIRLTSGKWDDIQPALNPAGDTLAFASNRGGRWDLYLLNLYSG
                     ELEQITDTPEFDGAPSWSPDGRWLAYESYREDTGSLEIFVRPLDGAQEPIQLTDDPGA
                     DFDPAWSPLGRQIAFVSTRSGENEIWLADLDLADNRFRNASRNNHQTESHPTWSPDGS
                     QLVWSTVGASGIHELSMVSTTSLSDPHPNPRMVGSGSWAAWSPTGQALLTILSTPNQY
                     FLTGYGFPENTITLPPLRINGAANGITWGRLTPGLNLTTTFASAAQTTPAALWQPALT
                     PMADIPAGRQRLVEVHDIQAPNAVLQDLVDESFIGLRERVAQAAGWDLLANLENAYVP
                     LTIPLGPGLEDDWLYTGRAFAFNPLPANAGWIVVLREDYGAQTYWRVMMRARYQDGTQ
                     GRPLDSLPWDFNARASGDPRAYETGGAFAKSIPAGYWIDFTRLAADYDWERLPALSTW
                     RSAYSAARFHEFVHTAGLDWQDAMLEIYPAEALVTPTVIVPTTGVPTRTLR"
BASE COUNT          445 a          559 c          609 g          440 t
ORIGIN      
        1 cgcaaagtgc gcgttggcac cccggttgtc ggcacaatga cggtaggcgt taccagtgct
       61 tcggccgggt agatttccag catggcatcc tgccagtcca gcccggcggt atgcacgaac
      121 tcgtgaaagc gggcagctga ataggcagat cgccaggtgg ataatgccgg caggcgttcc
      181 caatcgtagt ctgccgccag gcgagtaaaa tcgatccaat atccagccgg gattgatttt
      241 gcaaatgctc cccccgtttc gtaagcccgc ggatcgccgc tggcgcgggc attgaaatcc
      301 cacgggagtg aatccagcgg cctcccctgg gtgccatcct ggtaacgggc gcgcatcatt
      361 acccgccagt atgtctgcgc cccatagtcc tcgcgcagga cgacaatcca gcctgcgtta
      421 gctggcaggg ggttaaaggc gaaagcgcgg cctgtataca gccagtcatc ttccagaccg
      481 ggtcccaggg ggattgtaag aggcacgtag gcattctcca ggttcgccag cagatcccag
      541 ccggcggcct gggccacccg ctcacgtaag cctataaagg attcatcgac cagatcctgc
      601 aggacagcgt taggcgcctg gatatcatgc acctccacca gccgctggcg tccggctgga
      661 atgtccgcca tcggggtcag cgctggctgc cagagagcag ccggggttgt ctgggcagcg
      721 ctggcaaatg tcgtcgtcag gttcaatcct ggagtaagcc tgccccaggt aattccattg
      781 gctgcaccgt ttattcgtag tggaggcagg gtgatggtat tctccgggaa tccatagccc
      841 gtgaggaaat actggttcgg tgtgctcaaa atggtcagca gtgcttgccc ggtggggctc
      901 caggccgccc agctgccgct tcctaccatc cggggatttg gatgaggatc agataagctg
      961 gttgtggaaa ccatggaaag ctcgtgaatc ccgcttgcac caaccgtgga ccataccagc
     1021 tgagagccat ccggcgacca ggtgggatgg gattcggtct ggtgattatt tcggctggcg
     1081 ttgcgaaaac gattgtctgc caggtccaga tcggctagcc aaatttcgtt ctcgccgctg
     1141 cgcgtggaaa caaaggcaat ctgtcttccc agaggggacc aggccgggtc gaaatctgca
     1201 cccgggtcgt cggtcagctg gataggctct tgagcgccat ccagggggcg cacaaatatt
     1261 tccagactgc cggtatcttc ccgatagctt tcataggcca gccagcgacc atccggcgac
     1321 caggaagggg cgccatcaaa ctcgggagta tcggtaatct gttcgagctc gccgctgtac
     1381 agattgagaa gatacaaatc ccaccttccg ccccgatttg acgcaaacgc cagggtgtct
     1441 cccgctggat ttaatgccgg ttgtatatca tcccatttac ccgaggtaag gcgaataaat
     1501 ggacttaagc caggctggta ggcgaagagc tggctgtgac tgttttcatc caatgccagc
     1561 actgccaggc tgtcttgcag caccccaaaa gtctcgatcc aggtttcagg ctgttttaga
     1621 accagggtgg gagtgctcgt cggtgtcgct gtttcctcat tcgcagaaat cgtttgggta
     1681 atggcaggtg tggagaatag gctgattgtg ctgtttggaa tatccgcgct gggagtcaga
     1741 ctctgtccaa aggttggtgt gcgcgcctgc cagatagtcc aggcgaaaat acaacccagg
     1801 gttatcagcc caaggagcac caggcgcagc aggggtttag gctgggacaa tttttgtccc
     1861 gaagtctggt tttgagcagg caatcaacat ctccacgaag ggattacgcc aacctaatta
     1921 aaccatgttc gagggctggg acagggctaa tttacaaaac tggaagttga tgggtaagcc
     1981 gcctaagctg cctgatcctc aaatcccatc ttatacagct gataatacag gctttggagc
     2041 gagagtaact cgg
//
LOCUS       DATMXK010000200         2344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_150889, whole genome shotgun sequence.
ACCESSION   DATMXK010000200 DATMXK010000000
VERSION     DATMXK010000200.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2344
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150889"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            93..1523
                     /locus_tag="VN363_03765"
     CDS             93..1523
                     /locus_tag="VN363_03765"
                     /inference="COORDINATES: protein motif:HMM:TIGR00254.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="sensor domain-containing diguanylate cyclase"
                     /protein_id="HWQ83656.1"
                     /translation="MLRSFDENFPDLPADELIYHGIVDLQEDLICRFSCDGLLRLTYA
                     NPAFIQYFQLSPHAISVQSFPDVFPEDKRSFVWNSLKSVTSARPGFDVEYSAEVASGP
                     QVLLWWNIRGVFDGESLSGFQAIGHVVPGNTLANISSLQYTRQINAIYTATMALLTNL
                     DLESLIGQILDAAFDAIPGGQKGALHLLARETGELEMRAALGYQDNDPRIKKLNLKDG
                     VSYIARCVRERRPLLLKDMAPENGTSHEDNDQNTEIIHSALVAPLILGDEVLGALSLE
                     SPEDNAFNQADLDLISSFAATVTAAIRNAQLHSEVQRQAITDPLTELYNRRGFFEIGE
                     HEIERSHRFSRPLIAMMVDIDQFKVINDKFGHAVGDQVLQQISTRIRNNVRKVDIVGR
                     YGGDEFAILLPELDIFSAVNVAERLRKKVEEVPFQAGNQSIPATISVGVAKIVANMPN
                     LDSLLVRADHALYLAKNGGRNRVDIG"
     gene            complement(1564..>2344)
                     /gene="queA"
                     /locus_tag="VN363_03770"
     CDS             complement(1564..>2344)
                     /gene="queA"
                     /locus_tag="VN363_03770"
                     /EC_number="2.4.99.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tRNA preQ1(34) S-adenosylmethionine
                     ribosyltransferase-isomerase QueA"
                     /protein_id="HWQ83657.1"
                     /translation="LLLNRRNSHTWEALVGGKGFVPGRIIQIDDNLQGRISANLGGSR
                     RLVIFAKPIDELLPVIGQVPLPPYIHTHLDDPERYQTVYASQTGSAAAPTAGLHFTPG
                     LIDDLTTSGIRIARLTLHVGLDTFAPVTEDSPEEHTIHSEWCELNEAAAALINSARRN
                     GKRVIAVGTTSVRTLESAAQNTQNTEDIPPFSGATNLFILPGFTFRAVDAMVTNFHLP
                     RSTLIMLVSAFAGRAQILDAYQTAIQEKYRFYSFGDAMLIL"
BASE COUNT          552 a          581 c          637 g          574 t
ORIGIN      
        1 ttccaatggc gatcgttcgc agagataagg tcctttttgc catctgagac cgggagggga
       61 aaccaacgaa tatgccagaa ccaggtaata gaatgctccg ctcgtttgat gaaaactttc
      121 cggacctacc cgccgacgag ctaatttatc acggcattgt cgacctgcag gaggatttga
      181 tttgccggtt cagctgcgat gggctgctgc ggttaactta tgccaatccg gcttttattc
      241 aatattttca actttcgccc catgcgatct ctgttcaatc cttccctgat gtattccccg
      301 aggacaaacg cagctttgtc tggaatagcc tgaaatcggt cacaagcgcc agacctggct
      361 tcgatgtcga gtacagcgct gaagtagcct ccggcccgca agttttactc tggtggaata
      421 tcaggggcgt tttcgatggc gaatcgctga gtggattcca ggccattggg catgtcgtac
      481 ccggcaatac tctggcgaac atctccagct tgcaatatac tcgccagatt aatgctatct
      541 ataccgccac aatggcttta ttgaccaatc ttgaccttga atcactcatc ggccagatcc
      601 tggatgctgc ttttgacgcc attcctggcg ggcaaaaagg ggctttacat ctgctggctc
      661 gggagaccgg tgagctggag atgcgcgcag ctctgggata tcaggataat gatccccgga
      721 ttaaaaagct aaatttaaaa gatggggtaa gttatatcgc cagatgtgtc cgggagcgac
      781 gtccactctt gttgaaggat atggcgcctg agaatgggac gagtcatgaa gataatgatc
      841 agaataccga gatcattcat tcagcgctgg ttgctccgct gatccttggt gatgaagtac
      901 tgggcgccct atctttggag tcgcccgagg ataacgcctt caaccaggca gatctggatt
      961 taatatccag ttttgccgcc actgtaactg cagctatccg gaatgcccag ctgcacagtg
     1021 aagtgcagcg ccaggcaata accgatccgc taactgagct atacaaccgg cggggatttt
     1081 ttgaaattgg tgagcacgag attgaacgca gccatcgctt ttcacgccca ttaatcgcca
     1141 tgatggtgga tattgaccag ttcaaagtaa tcaatgataa gtttgggcat gcggttggag
     1201 atcaggtgct gcagcaaatc tcgacccgga tccggaataa tgtccgcaaa gtggatatcg
     1261 tcggccgcta cggcggggat gaatttgcca tcttgctccc agagctggat atcttttcag
     1321 cagtgaatgt tgccgaacgc ctgcgtaaga aagtggaaga agtacccttc caggctggaa
     1381 accaatcaat cccggcgacg atcagtgttg gcgtcgccaa gatcgtggcg aatatgccca
     1441 atttagattc attactggtg cgtgctgatc acgctctcta cctggcaaaa aatggcggca
     1501 gaaaccgggt tgatataggc tgaatttctg ctctgtaatc cctgggggta tctttatacc
     1561 ggtttacagg attaacattg catcgccaaa tgaataaaag cggtactttt cctggattgc
     1621 tgtttgatag gcatccagga tctgcgctcg ccctgcgaac gctgaaacta acatgattaa
     1681 ggtagaacgg gggaggtgga aattagtgac catggcatcc acagcccgga aggtaaaccc
     1741 gggcagaata aataggttgg ttgcaccgct gaatggcggg atatcttcag tgttctgggt
     1801 gttctgagcc gccgattcaa gcgtccgtac cgatgtagta ccgaccgcga ttacccgttt
     1861 accgtttcgc ctggccgagt tgatgagcgc ggcggctgcc tcgtttaact cgcaccattc
     1921 gctgtggatg gtgtgttctt ccgggctatc ttcggtgact ggcgcaaaag tatccagacc
     1981 gacgtgcagg gtcagcctgg caatgcgtat accagaagtg gtgagatcgt caatcaaacc
     2041 gggcgtaaaa tgcaggccgg cggttggcgc agccgcagac cccgtctggc tggcatatac
     2101 tgtttgatag cgctcgggat catccaggtg ggtatggatg tatggcggca ggggtacctg
     2161 cccgatcacc ggcaggagct catcgatcgg tttggcgaaa atgaccagcc gccgtgaacc
     2221 gcccagattg gcagagatcc ttccctgcag gttatcgtct atctggatta ttcgcccagg
     2281 tacaaaacct ttcccgccaa ccagggcttc ccaggtatga gaatttcgcc ggttgagcag
     2341 cagc
//
LOCUS       DATMXK010000201         2368 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161274, whole genome shotgun sequence.
ACCESSION   DATMXK010000201 DATMXK010000000
VERSION     DATMXK010000201.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2368)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2368)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2368
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161274"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            329..>2368
                     /locus_tag="VN363_03775"
     CDS             329..>2368
                     /locus_tag="VN363_03775"
                     /inference="COORDINATES: protein motif:HMM:NF024180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAT domain-containing protein"
                     /protein_id="HWQ83658.1"
                     /translation="MIGYAELEISLHRRETGSYAIDFRFSQPDSDADIRLGQDQTTQT
                     GIDLDALLALTHSPDKYGEKLTQDFFAEPAVLAAFTQAVSSAQSQNRPLRLRLVIGPS
                     APELHSVRWETMNNPLDNTPLCTNENIIFSRYLSSLDWRPVRLRPRDDLSALVLVANP
                     SNLDEYNLSAVDVPGEIERARSGLGNIRLSLIPDPDKAGQATSDQLIDQLREKEFDIL
                     YLVAHGALVKNEPWLWLEDQEGKVARTSGIELVTRLKELQKRPRLVVLASCQSAGKNA
                     GDALQALAPRLAEAGIPAVIAMQGNISMETVADFMPAFFEELRRDGQIDRAITVARGK
                     VRARADYWMPALFTRLKSGRIWYVPGFGEDRKGFEKWPALMRSIKRGQCTPVLGPGLY
                     EPYLGAPREIAQRWAEAYHYPMAPHERESLPQVAQYLTINQYQRAPYDELEEYLQGEV
                     QSRFTASLPPELRQGRMPLDQLLDVVRARVHKDHPFEPHQALAQLPLPIFITTNHDGL
                     LTSALKDAGKDPQPVLCPWNEYIEQIESVYDREPDYFPTPERPLVYHLFGRIDEPDSL
                     VLTEDDYFDFLIGITGNKDLIPSAVRRALADTALLFLGFQMDDWNFRVLFRSILSQQG
                     GSRRDRYAHIAAQVEPEEGRNLEPERARSYLENYFAKGADISLFWGTPEDFVKELI"
BASE COUNT          508 a          680 c          638 g          542 t
ORIGIN      
        1 caaattcccc ttgcgtattt agcagtattc gggtaaagta aggcataaat tgctcaacca
       61 gaattccatt cctgcctaat tgagctgtcc aattaaccga tcaatttgtc tcccactgtc
      121 gggtttgtgg ggaagaacaa accctctttc aggattaacg cttccctcat agcgtggtaa
      181 gcgggggtaa tctgggcgta aacaccaacc ctgtccgtct attgcaattc catcctggtc
      241 tgtctcgttg tcgcagacgg tatagaaaat tcgggcatgg atcctatcca tgctgctcat
      301 ttgctcctcc agagagggga atatgcctat gattgggtat gctgaactcg agatcagcct
      361 gcatcggcgg gagactggct cgtatgccat cgattttcgc ttctcccagc cagattctga
      421 cgccgatatt cgccttggac aggatcaaac cactcagacc ggtatcgacc tggatgccct
      481 tctggcattg acccacagcc cggacaaata tggcgaaaaa ctgactcagg atttttttgc
      541 tgagcctgcc gtgctggctg cattcacgca ggcggtcagc tcagcccaat cacagaacag
      601 gccactgcgg ttacggctgg taatcggtcc cagtgcgcca gagcttcaca gtgtccgctg
      661 ggagacgatg aataacccgc tggataacac ccctctttgc actaacgaaa atatcatctt
      721 ctcgcgttat ctgagcagcc tggattggcg gccggtccgt ttacgcccca gggacgacct
      781 gtctgctctg gtcctggttg caaacccatc taacctggat gaatacaacc tgtctgccgt
      841 cgatgtaccc ggagagatcg agcgtgcccg gtctggcctg ggaaatattc gcctcagcct
      901 gatcccggat cctgataaag ctggtcaggc tacttccgat cagctcatcg accagctccg
      961 ggaaaaggaa tttgatatcc tgtatctggt tgcgcatggc gcgctggtca agaatgaacc
     1021 ctggttgtgg ttggaagatc aagaaggcaa ggtagcccgc acctcgggca tcgagctggt
     1081 tacccgtctc aaggagctgc agaaaagacc caggctggta gtgctggcat cctgccagag
     1141 cgcgggaaaa aatgccggcg atgccttaca ggctttagcc cctcgcctgg cagaagctgg
     1201 gatccccgcg gtaattgcca tgcagggaaa tatcagcatg gaaacggtcg ccgatttcat
     1261 gccggctttc ttcgaagagc tccgccggga tgggcagatc gatcgtgcga tcaccgtggc
     1321 gcgcggtaag gtgcgcgctc gcgccgatta ttggatgccg gccctcttca cccgtttgaa
     1381 gagtgggcgg atctggtatg tgcccggttt cggtgaagac cggaaaggtt ttgaaaaatg
     1441 gcctgccctg atgcgcagca tcaagcgtgg gcagtgcact ccagtcctgg gtccagggct
     1501 ttatgagcca tatctgggcg caccgcgcga gattgctcag cgttgggcag aggcctacca
     1561 ctacccgatg gcgcctcacg aacgcgaatc tttacctcag gttgcccaat atctgacgat
     1621 caaccagtac cagcgcgccc cctatgacga gctcgaagaa tacctgcagg gtgaggtcca
     1681 gtctcgcttt acggcgagct tgccgcctga gctccgccag ggacggatgc cgttggatca
     1741 gctgctcgat gttgtcagag cccgcgtcca caaagaccat ccgttcgagc ctcaccaggc
     1801 gctcgcccag ctgcccctgc ccatctttat caccaccaat catgacgggc tgctcacttc
     1861 agccctgaaa gatgctggca aggatcccca gcccgtcctt tgcccctgga atgagtatat
     1921 cgagcaaatc gagtcggtct atgaccggga gccggattat tttcccactc ctgaaagacc
     1981 actggtctat cacctgttcg ggcggatcga tgaaccggac tcactggtgc tcaccgaaga
     2041 tgattacttc gattttctca tcgggataac cggtaacaaa gaccttatcc cctctgccgt
     2101 tcgccgggcg ctggcagata ccgccctcct gttccttggc ttccagatgg atgattggaa
     2161 tttccgggtc ttattccgca gcattctctc ccagcagggc ggcagccggc gtgaccggta
     2221 cgcccatatc gccgcccagg tggaacctga agaaggtcgc aacctggagc ctgagcgagc
     2281 tcgatcttac ctggaaaact attttgccaa gggggctgat atcagtctgt tttggggtac
     2341 cccggaagat ttcgtcaaag agttaatc
//
LOCUS       DATMXK010000202         2317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_149093, whole genome shotgun sequence.
ACCESSION   DATMXK010000202 DATMXK010000000
VERSION     DATMXK010000202.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2317
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_149093"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..783)
                     /locus_tag="VN363_03780"
     CDS             complement(<1..783)
                     /locus_tag="VN363_03780"
                     /inference="COORDINATES: protein motif:HMM:NF018798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1385 domain-containing protein"
                     /protein_id="HWQ83659.1"
                     /translation="MPSYGGQAVIEGVMMRGAQGVAIAMRAPNQEIIIHTEKLGRIYH
                     SPIARIPFLRGLIGLWDALGLGIRALTIAANTQTGEDEKIEGPALYGTLAISLVFGIG
                     LFFLAPAALGQLSEQLVNWSAWWGNVAEGVIRLGLLVGYIALIGRMPDIKRVFMYHGA
                     EHKTINAFEAGAELTPENVARYSVEHPRCGTAFLLTLVLLSVLVFSLLGPLPWFWRLA
                     SRILLLPVLAGLAYEFIRWTARHLDSPLVRLIIKPNLALQHMT"
     gene            complement(993..>2317)
                     /locus_tag="VN363_03785"
     CDS             complement(993..>2317)
                     /locus_tag="VN363_03785"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:NF005914.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dipeptidase"
                     /protein_id="HWQ83660.1"
                     /translation="GQILQNLKDFVAIPSISTDTEATPDIQRAAEWVAGQLQGMGIEN
                     VAILPTSGHPVVYGEWLNAGADRPTVLVYGHYDVQPVEPLELWDTPPFEATEKGAHLY
                     GRGASDMKGQVVICLSAVQALLETSSLAVNLKFLIEGEEEIGSPALEKFIPEHKALLA
                     CDVALNADTGMMGAEIPTITYGLRGLGYYELWVYGPRQDLHSGLFGGAIHNPAQVLCD
                     LIAGMHDDQGRVTLPGFYAAVRELSPEERAELARLPHGDEFVLEQSGAPALYGEAGYT
                     NTERIGARPTLEINGILSGFTGVGQKTVLPSKAMAKISMRLVPNQDADQVLEQLRQYL
                     VAKAPPTVTWELKKLSGGDPSISDIHHPAVAAMVKALETVWGVRPLYRREGGSVPVVA
                     KFQKVLGIESVCTGFGLPDDNLHAPNEKLHLPTFYRGLDAAVHFFLNY"
BASE COUNT          499 a          716 c          668 g          434 t
ORIGIN      
        1 ggtcatgtgc tgtaaggcca ggttgggctt aatgatcaaa cggaccaggg gtgaatccag
       61 atgccgggcg gtccagcgga tgaactcgta ggctaacccg gccaggacag gcagcaagag
      121 gatccggctg gcgagacgcc agaaccaggg cagcggtccc agcaggctga ataccagcac
      181 cgagagcagt accagggtca gcaggaaagc cgtcccacag cgcgggtgct ctacggaata
      241 gcgggcgaca ttttcggggg tcagctctgc gccggcttca aaagcgttga tcgttttatg
      301 ctcggctccg tggtacatga acacgcgctt aatatcgggc atcctgccaa tcagcgcgat
      361 gtaacccacc agcaaaccca ggcggataac gccttcggcg acattgcccc accaggcgct
      421 ccaattcacc agctgctcgc tcaactggcc cagggcagcc ggcgccagga agaacaggcc
      481 gataccaaaa accagggaga tcgccagcgt gccgtacaga gccggtcctt cgatcttttc
      541 gtcttcgccg gtctgggtgt tggcggcgat cgtcagagcg cggataccca gacccagggc
      601 atcccacaaa ccgatcaaac cgcgcaggaa gggaatcctg gcgatcgggc tgtggtagat
      661 cctgcccagc ttctcggtgt ggataataat ttcttgattg ggagcgcgca tggcaatcgc
      721 gacaccctgc gcaccgcgca tcatgacgcc ttcgatcacc gcctggcccc cgtaagaagg
      781 caaagattca ctcataagca gagattgtac gatctaccgc ggggaccgtc aagctgaaat
      841 ccaaatcact caccgatttc tcagacaggg cagggtgaat tgctcgtgaa tgggcagggg
      901 cggcccgcca ccgccaggca aaccctgatc caggaaagca accaaccaga ccaggattgc
      961 cacggcagcc agggggctgc ccctgagtca ggctaataat ttaagaagaa atgcaccgcg
     1021 gcgtcgaggc cgcgataaaa agtgggcagg tgcagcttct cattgggggc gtgcaggtta
     1081 tcatctggca gaccaaagcc tgtgcagacc gattcgatgc ccagcacctt ctggaatttt
     1141 gcaaccaccg gtacgctgcc gccttcacgc cggtagagcg ggcgcactcc ccagacagtc
     1201 tcgagagctt ttaccatggc tgccaccgcc gggtggtgga tgtcgctgat gctgggatcg
     1261 ccgccagaga gctttttcaa ttcccaggtc accgtgggtg gcgccttggc taccagatat
     1321 tggcgcagct gctccaatac ctggtctgcg tcctggttgg gcaccaggcg catggaaatt
     1381 ttagccatcg ctttggatgg tagcacggtc ttctgaccca caccggtaaa accggaaagg
     1441 ataccattga tctccagggt aggccgggcg ccgatccgct cggtattggt gtaacccgcc
     1501 tcaccataca gcgccggggc tccagactgc tcgaggacaa attcatcccc gtggggcaga
     1561 cgggccagct cggcgcgttc ttccgggctc agctcacgca ctgcagcgta aaaaccgggc
     1621 agagtcaccc gcccctgatc gtcatgcatg ccggcaatca gatcgcacag cacctgggcc
     1681 gggttgtgga ttgccccacc aaacagcccg gaatgcaggt cctgcctggg tccgtacacc
     1741 cacagttcgt aataacccag gccgcgcaag ccataagtga tcgtggggat ctccgcgccc
     1801 atcatgccgg tatcggcgtt cagggcaacg tcacaggcca acaaagcctt gtgctcgggg
     1861 ataaatttct ccagggctgg cgagccgatc tcctcttcgc cctcgatcag gaattttaaa
     1921 tttacggcga ggctgctcgt ctccagcagc gcctggactg ctgacaggca gattaccacc
     1981 tggcctttca tatccgatgc gccccgccca tacaggtgcg cgcctttttc agttgcctca
     2041 aagggcgggg tatcccacag ctccagcggc tcaaccggct gcacatcata atgcccgtat
     2101 accagaaccg taggccggtc agccccggca ttcagccact cgccatatac cacgggatgc
     2161 ccgctggtag gcaggatggc aacgttctcg atacccatcc cttgcagctg ccccgccacc
     2221 cattcagcgg cgcgctggat atccggtgtg gcttctgtgt ccgttgaaat cgaggggatt
     2281 gcgacaaaat ccttcaggtt ttgcaggatc tgtccct
//
LOCUS       DATMXK010000203         2261 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_74519, whole genome shotgun sequence.
ACCESSION   DATMXK010000203 DATMXK010000000
VERSION     DATMXK010000203.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2261)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2261)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2261
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74519"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..654
                     /locus_tag="VN363_03790"
     CDS             <1..654
                     /locus_tag="VN363_03790"
                     /inference="COORDINATES: protein motif:HMM:NF013791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0070069 - cytochrome complex [Evidence
                     IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0019646 - aerobic electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome ubiquinol oxidase subunit I"
                     /protein_id="HWQ83661.1"
                     /translation="PMKIAAAEALWETEDPASFSVLTIGDLSQTKEVFSIRIPKVLSL
                     LSYNQLWGEVRGIYDLQAEYVQKYGPGNYTPPVAVIYWSFRIMVGAGMLMIGLSIYAL
                     FISRGDRFAVKPKILSLFTFAIALPYLANSFGWIMTEIGRYPWVVYGLMKIEDGVSPI
                     VGPGKALASLLGFTVLYGALMGATIYLLSKYARAGIQVEPASSMPDDALPSLVGAQR"
     gene            673..1707
                     /gene="cydB"
                     /locus_tag="VN363_03795"
     CDS             673..1707
                     /gene="cydB"
                     /locus_tag="VN363_03795"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560673.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016682 - oxidoreductase activity, acting
                     on diphenols and related substances as donors, oxygen as
                     acceptor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome d ubiquinol oxidase subunit II"
                     /protein_id="HWQ83662.1"
                     /translation="MDLNIIWFVLIAVLYVGFFVLEGFDFGVGILVPVLSKDKDPEMV
                     DLKRRTMINTIGPHWDGNEVWLLTAGGATFAAFPHWYATMFSGFYLAFFLLLVALILR
                     GVAFEFRSKDENPRWRKFWDWSIFTGSLVPALLLGVAFGNLVKGVPIDQNMQYAGTFM
                     DLLNPYALLAGITLVFGCMLYGALFLSLKTTGELRDQASALARKLWPVVLVLLLGLMA
                     ATYIATDLLNELGVNPGAVRLAGLAAIVLTGYFLQKGHQGWAFIMVATTIATTIITAF
                     MIMFPNVMISSLNPEWSLTIYNASSSPYTLRVMSIVALIFVPIVLVYQGWSYWVFRKR
                     LETKVEGLTY"
     gene            1785..>2261
                     /locus_tag="VN363_03800"
     CDS             1785..>2261
                     /locus_tag="VN363_03800"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter transmembrane domain-containing
                     protein"
                     /protein_id="HWQ83663.1"
                     /translation="MNLNKRLLSLARQSRIFLGLTIVFGFSAGLLAVLQARGVSQVVN
                     QVFLEGRNLTQVDTWLRWLLVIILGRAGLAWASEISANAIAVRVKTSLRKHLFGHLLS
                     LGPFYAQAERSGELTQTVVEGTEALDAYFSQYLPQLVLAALIPVTFLFFVFPLDPLS"
BASE COUNT          450 a          537 c          634 g          640 t
ORIGIN      
        1 ccgatgaaga tcgctgccgc tgaggcatta tgggaaacgg aagatccggc ctcgttttca
       61 gtattgacga tcggagattt atcccaaacg aaagaggtat tctccattcg aattccaaaa
      121 gtgctcagcc tcctatcata taaccagctt tggggtgagg tccgaggaat ttatgacctt
      181 caggctgagt atgtgcagaa atatgggccg gggaattaca cgccacccgt tgcggtgatc
      241 tattggtctt tccggatcat ggtcggcgca ggtatgctta tgatcgggct atccatttat
      301 gccctgttta tctctcgggg agacaggttc gctgttaaac cgaagatctt gagcctgttt
      361 acttttgcaa ttgctttacc ctatctggcg aattcgttcg gatggatcat gaccgagata
      421 gggcgttacc cctgggtcgt atacgggctg atgaagattg aagatggagt ttccccgatt
      481 gtcggtcccg ggaaagcgct ggcaagcctg cttggattca cggttctcta tggcgcactg
      541 atgggtgcca ccatttatct gctgagtaaa tatgccagag ctggcattca ggttgagcca
      601 gcgtccagta tgccggatga tgccctgccc tcactggttg gggcgcaaag ataagcggag
      661 cggaggctac ttatggatct caatatcatc tggtttgttt taatagcggt tttatatgtt
      721 ggtttctttg tcctggaggg ttttgacttc ggggtaggga tactggtgcc ggtgctttcg
      781 aaggacaagg accccgagat ggtggacctt aagcggcgca caatgatcaa tacgatcggg
      841 ccgcattggg atggtaacga agtctggctg ctgactgcag gtggggcgac attcgcggcg
      901 tttccccact ggtatgccac catgttcagt gggttttatc tggccttctt tttgctgctg
      961 gtcgcgctga ttttgcgtgg cgtggctttt gagtttcgca gtaaggacga aaacccgcgc
     1021 tggcggaagt tttgggattg gtcgatcttt accggaagtc tggtgcctgc cttgttatta
     1081 ggggtggctt ttggcaatct ggtgaaggga gtgcccattg atcaaaacat gcagtacgcc
     1141 ggaacgttta tggatcttct gaatccctat gctctgttgg ctggaattac gctggtcttc
     1201 gggtgcatgc tgtatggagc gctctttttg agccttaaaa ccacagggga gcttcgtgat
     1261 caagcctcgg ccctggcgcg taagctctgg ccagttgtac tggttttact tttaggcttg
     1321 atggcagcaa cttacatcgc tacagatttg ctcaacgagt taggtgtgaa tccgggtgca
     1381 gtgcggcttg ccggcctggc tgcgatcgta ctcaccggtt atttcttgca gaaaggccac
     1441 cagggctggg cattcatcat ggtagcgaca acgatagcca ccacgattat cacggccttc
     1501 atgatcatgt tccccaacgt gatgatttct tccttgaacc ccgagtggag tctgacaatc
     1561 tataatgcct cctccagccc ctatacgctc agagtgatga gtattgtggc tcttatcttt
     1621 gtccccatcg ttttggttta tcagggctgg agttactggg tattccgcaa acgactggag
     1681 accaaggtgg aggggcttac ctactagctt gttgacctgg tttgccaata acgaggtggg
     1741 gcagtttgga gttacttgcc ccacctttat tgtgaaatca cacgatgaac ctgaacaaac
     1801 gtttgctttc cctggcgcgc caaagccgga tatttttggg gctgaccatc gtttttggtt
     1861 tcagcgcggg gctattggct gtgctccagg ccaggggcgt cagtcaggtc gtcaaccagg
     1921 tcttcctgga aggccgaaat ttaacccagg tagacacctg gctgcgctgg ctgctggtaa
     1981 tcatcctggg acgagccggg ttagcctggg cgagtgagat ttcggccaac gccattgctg
     2041 tccgcgtaaa gaccagtctt cgcaaacatc tttttggaca tttgctctct ttaggcccgt
     2101 tctatgcaca ggctgagcgc agtggtgaat taacccagac tgttgtcgag gggaccgaag
     2161 ccttggatgc ttacttcagc cagtacctgc cccaattggt gctggctgct ttaattccag
     2221 taacctttct gttctttgtt ttccccctcg acccattatc t
//
LOCUS       DATMXK010000204         4379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_70705, whole genome shotgun sequence.
ACCESSION   DATMXK010000204 DATMXK010000000
VERSION     DATMXK010000204.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70705"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..631
                     /gene="radC"
                     /locus_tag="VN363_03805"
     CDS             <1..631
                     /gene="radC"
                     /locus_tag="VN363_03805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559811.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWQ83664.1"
                     /translation="HLGAQSLSNAELIAILLRVGVPGENAVQVGQRLLQHFGGLAGLH
                     RADFEEVCSQHGLGAAKASQIKAAIELGRRLTLESPEERPSIHSPQDAADLLMYEMGA
                     LEQEHLRVILLDTRNRVLHIEEVYHGSLNSSQVRVGELFKTAIRRNAAAIIVAHNHPS
                     GDPSPSPDDIAITRAIVQAGKLLDVDVLDHVVIGRGRFVSLKERGLGFG"
     gene            complement(637..933)
                     /locus_tag="VN363_03810"
     CDS             complement(637..933)
                     /locus_tag="VN363_03810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560392.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylphosphatase"
                     /protein_id="HWQ83665.1"
                     /translation="MVESEQIRLHAIVEGTVQGVGFRYFVQDAAKLLGLNGWVRNRRD
                     GTVEIQAEGERSRLEQLLAAVRRGPRSAHVMGVDFAWQEYSGEFSDFRVRQTSG"
     gene            complement(938..2197)
                     /locus_tag="VN363_03815"
     CDS             complement(938..2197)
                     /locus_tag="VN363_03815"
                     /inference="COORDINATES: protein motif:HMM:NF024876.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidase MA family metallohydrolase"
                     /protein_id="HWQ83666.1"
                     /translation="MNSHKPIPSVFRWLISLFMMGCLLIPSIAVQAQATVSNTSVEYK
                     FGEQISFKALVQSPLPIEKAFVIFRVSGESVTRSSEAEIIYQDGTTYQIASTYPLDNQ
                     PLRAFTQVEYRFQARLQGGEEFNSPSLSFEYLDNRVEWQNLEQAPFHIAWYAQDDIAF
                     GDNALKTAQEGLKKINSLLPIQTPEHIDIYIYPNASTLQEVLILSGQDWIAGHADPDL
                     GVILAAIPSRPEQSLVMDQIIPHEMMHILVYETVREGYDNLPTWLVEGLASLAELYPN
                     PDYQILLNNALERDTLLKMEDLCAPFPRDASSALLAYAQSASFTRFLHQQFGASRMDD
                     LMQGYTDGLECSRGVEITLGTNLKQLERQWREDVLGEDPARAAAENLLPWLLLALVVL
                     VAPIGLALSRMSRKSDQPAKLDPRDIR"
     gene            complement(2420..3526)
                     /locus_tag="VN363_03820"
     CDS             complement(2420..3526)
                     /locus_tag="VN363_03820"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWQ83667.1"
                     /translation="MTTLKSPKRPADIPFQQVLDAYLDSETPFPPRFLNRLSDLSHAD
                     LEALNKVWQEAPSWRRQAIMEDIESLGEADTLLSFEALAQHALQDDEASVRVPAVRVL
                     SEFENPAHASRFMALLESDPDTEVRAAAASALGYYVYLGELDELDPLKKAEIETCLLK
                     YFHGNHADLIRRRALEALGYSSLEEVQPLIQQAYASENRDWKASALYAMGRSASEIWL
                     PQVLESLESILPQIRAEAARAAGEIDAHVAVERLIELLDDPDEDVMTAAIWSLSQLGG
                     EGVGDALAKVFKRTDDDELAEWIDSAMENLAFTEGVQAFEMLDLAEDELDDDDDEDDE
                     ELEDDELENVDEYLESLDDEDRDLFDLEAEDEEF"
     gene            complement(3529..>4379)
                     /locus_tag="VN363_03825"
     CDS             complement(3529..>4379)
                     /locus_tag="VN363_03825"
                     /inference="COORDINATES: protein motif:HMM:NF014244.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="M50 family metallopeptidase"
                     /protein_id="HWQ83668.1"
                     /translation="IPLGGFVRPKGENDPTIPGGLASASPWKRLGVLFAGPGINILTG
                     ILLAILLFYTLGEPILDKVRVDEVAADSPAMQAGLLAGDIITEVDGVRIDSMNVLQET
                     IQTRLDKSITVSVDRSGEKVSVTLTPRSNPPANQGAIGVLLGNPTRPIPVTTAITGGF
                     SASFEYMRAVLALPVRLIRGENSPEDGRAFVGYKGMFDIYQQIRNPLYFFMAVTFSLG
                     LINLFPIPALDGGRILLTLPEIILKRRIPAQYENMIHLVGFAVLLLLLIYINIQDFVN
                     PVVLPK"
BASE COUNT         1027 a         1155 c         1189 g         1008 t
ORIGIN      
        1 ccacctgggt gcacaatcgc tctctaatgc tgagttgatc gccatcttgc tgcgagtagg
       61 tgtacccggc gagaatgcgg ttcaggtggg tcagcgcttg ttgcagcatt tcgggggatt
      121 agcgggtctg catcgggcgg attttgaaga agtgtgcagc cagcacgggc tgggcgctgc
      181 caaagcctct cagatcaagg ctgccatcga gctggggcgc cgtctgaccc tggaatcacc
      241 cgaggagcgg ccaagcatcc acagcccgca agatgccgct gacttgctga tgtatgagat
      301 gggtgccctg gaacaggagc acctgcgcgt gattctgctc gatacgcgca accgggtgct
      361 tcacattgag gaggtttacc atggttcgct gaacagctct caagtgaggg ttggcgagtt
      421 gttcaagacg gctatccggc gtaatgccgc cgcgatcatc gtcgcccaca accatcccag
      481 cggagatccc tcacccagtc cggatgacat tgctattacg cgcgccatcg tccaggcagg
      541 taagctgctg gatgtggatg tgttggatca cgtagtgatc gggcgcgggc gatttgtgtc
      601 gttaaaggaa agaggcctgg ggtttggata ataagattat ccgctggttt gtctcacccg
      661 aaaatcgcta aattcaccac tgtattcttg ccaggcaaag tctaccccca tcacgtgcgc
      721 cgagcgagga ccgcggcgaa ctgccgctaa aagctgctcc agccggctgc gctcgccttc
      781 tgcctggatc tccactgtac catccctgcg attgcgtacc caaccattga gtcccagaag
      841 cttagcagca tcctggacaa aatagcgaaa acccaccccc tggacggttc cttcgacgat
      901 ggcgtgcagt cggatttgtt ctgattctac caagcgctta cctgatatcc cttggatcca
      961 gttttgccgg ttgatcactc ttacgcgaca tgcgactgag agccaaccca atcggagcaa
     1021 ccagcacgac cagtgctagt agcagccagg gaagcaggtt ctcggcagct gcccgggcgg
     1081 gatcttcgcc taaaacatcc tctcgccact ggcgttcgag ctgtttcaga ttggtcccca
     1141 gggtgatctc caccccgcgc gaacactcta aaccatctgt atagccttgc attaagtcgt
     1201 ccattcggct ggcaccaaac tgctggtgca ggaaacgggt aaaggaggcg gattgagcgt
     1261 atgccaataa ggcgctggag gcatcgcgcg ggaaaggtgc gcataggtcc tccattttta
     1321 aaagtgtatc acgctccagg gcgttattca ataagatctg gtaatccgga ttgggataca
     1381 gctcggcaag cgatgccagg ccttccacca gccaggtcgg caggttgtca tagccttccc
     1441 tcacagtttc atagaccagg atatgcatca tctcgtgagg aatgatctga tccatgacca
     1501 gcgattgttc tggacgagat gggatcgccg ccaggattac gcctaaatcg gggtctgcgt
     1561 gcccggcgat ccaatcctgg ccactcaata tcagcacttc ttgcagagta ctggcattgg
     1621 ggtagatgta aatgtcgata tgctctggcg tttgaatcgg cagcaggctg ttgatctttt
     1681 tcaacccttc ctgggcggtc ttgagggcgt tatctccaaa agcaatatca tcctgagcat
     1741 accaggcgat gtgaaacggc gcctgttcaa gattttgcca ttcaaccctg ttgtcgaggt
     1801 attcgaagga taaggagggg ctgttaaatt cttccccgcc ctggaggcga gcctgaaagc
     1861 gatattctac ctgggtaaaa gcgcgtaagg gctggttatc cagcgggtat gtgctggcta
     1921 tctggtaggt tgtcccatcc tggtaaatta tttcggcctc gctggaacga gtcacacttt
     1981 cgccgctcac cctgaatatg acaaaagctt tctcgatggg gaggggtgat tggacaagcg
     2041 ccttaaaact gatctgctcg ccaaatttgt attcaacgct tgtattggaa actgtcgcct
     2101 gagcctggac ggcgatactc gggatcagga gacatcccat catgaatagg ctgataagcc
     2161 agcgaaagac cgatgggata ggtttgtggc tgttcattca gcctccgaaa ctcgttctcg
     2221 acggataagg tgtttcgaaa ccgccagcag gacggtaaca ggatcaccag caaagctgat
     2281 ttgccggtca ttaattaaat tgcaagattc ttgcctgaca tgtcagagct ggattctccg
     2341 attaataaaa caacctgctg cggtggagtt cgtttcgcgg caggcttgta gattcaactg
     2401 atattagtgc agctgatgcc tagaattcct cgtcttcagc ttcgagatcg aacaggtccc
     2461 tatcctcgtc gtccaggctt tcaagatatt cgtccacgtt ttccagctcg tcatcttcaa
     2521 gctcttcatc gtcttcgtcg tcgtcatcgt caagctcatc ttccgctaaa tccaacattt
     2581 cgaatgcctg aacgccttca gtaaaagcca ggttttccat ggcggagtca atccattccg
     2641 ccagctcgtc atcatcagtc cgcttgaata ctttcgcaag agcatcaccg accccctcac
     2701 cgccaagctg ggatagcgac cagatcgcag cggtcatcac atcctcatcc ggatcgtcaa
     2761 gaagctcgat taagcgctcg acagccacat gggcatctat ttcgccagcc gcgcgggcag
     2821 cttcggcgcg gatttgaggc agtatagatt cgagagattc gagcacctga gggagccaga
     2881 tctcactcgc cgagcgaccc attgcgtaga gggcggaggc tttccaatcc cgattctcgg
     2941 aggcataagc ttgctggatc aatggttgta cttcctcaag gctggagtag cctaatgctt
     3001 ccagggcacg ccggcggata agatcggcat gattgccatg aaaatattta agcaagcagg
     3061 tttcgatttc ggccttcttg agtggatcca gctcatccag ctcacccaga tagacatagt
     3121 atcccagggc agacgctgcc gcggcgcgga cttcagtgtc tggatcggat tcgagcagcg
     3181 ccatgaaacg ggatgcgtga gcaggatttt caaattcaga cagcactcgc acggctggca
     3241 cgcggacgct ggcttcgtca tcttgtagcg cgtgttgagc taaagcctca aaagaaagca
     3301 gcgtgtccgc ttcacccaga ctctcgatat cttccatgat ggcctggcgg cgccaggaag
     3361 gcgcttcttg ccagactttg ttaagggcct ctaaatcagc atggctcaga tcggataacc
     3421 ggttaaggaa gcggggtgga aaaggtgttt cagaatccag ataagcatcc aatacctgtt
     3481 ggaagggaat gtctgctgga cgtttggggg atttaagtgt cgtcattgtt acttgggtaa
     3541 aaccaccgga ttaacaaaat cctgaatatt gatgtagatc agcaagagca agagaaccgc
     3601 gaatccaacc aggtggatca tattctcata ttgggcaggt atacggcgtt tgaggatgat
     3661 ctcgggcaag gtgagcagga tccgcccacc atccagggca gggatcggaa agagattgat
     3721 taaacccagg gaaaacgtaa ccgccatgaa gaaatacagt ggattgcgga tctgctggta
     3781 aatatcgaac atgcctttat agccaacgaa tgcacgcccg tcttcaggcg aattctcacc
     3841 tctgatcaaa cgaaccggaa gagccaggac agcgcgcata tattcaaagg acgccgaaaa
     3901 gccgcctgta atggctgtgg tgaccggaat aggccgagta ggatttccca gcagaacacc
     3961 aatcgcgcct tgattggcag gcgggtttga gcggggagtc aacgtgacac tgactttttc
     4021 tccactgcga tcaacgctga cagttatcga cttgtccagg cgcgtctgga tagtttcttg
     4081 aagcacattc atgctgtcga tgcgtacgcc atccacctcg gtaataatat cgccagcgag
     4141 caagccagct tgcattgcgg gggaatcggc ggctacttcg tcgacgcgca ccttgtcaag
     4201 aataggctca cccagtgtgt agaagagcag gatcgccaga aggatgccgg tgaggatatt
     4261 aatcccggga cccgcaaaca atacccctaa acgtttccat gggctggcag atgccagacc
     4321 acctggaata gttggatcgt tttcaccttt cgggcgcaca aagccaccca ggggaatcc
//
LOCUS       DATMXK010000205         3002 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_46354, whole genome shotgun sequence.
ACCESSION   DATMXK010000205 DATMXK010000000
VERSION     DATMXK010000205.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3002)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3002)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3002
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46354"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VN363_03830"
     CDS             complement(<1..389)
                     /locus_tag="VN363_03830"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ83669.1"
                     /translation="MTRTAVNYDQIAPTNNCRFQGEKSQPILDALRSLARQIHAGAVL
                     EAGCGTGFWLAGLEVAFAKQAGQKFFGLDRSIGMLRQASLRKHGFYLVQGQAEAFPFS
                     ESSFDLIYCVHALHHFSNQPEFITSCLR"
     gene            complement(462..791)
                     /locus_tag="VN363_03835"
     CDS             complement(462..791)
                     /locus_tag="VN363_03835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83670.1"
                     /translation="MEKAQTSFGPIQAGILLLALATGLIHFTLAIPDRLIMFYLNGIA
                     YVVLAVALFLPQFKSYRTWIRYALMALTLITIIAWVAIGMRTPLGYIDKIIEVALLAL
                     LWMDRGR"
     gene            complement(794..1822)
                     /locus_tag="VN363_03840"
     CDS             complement(794..1822)
                     /locus_tag="VN363_03840"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWQ83671.1"
                     /translation="MKAFARTWSRQPFWLYGIWAAALGLFILSIKSIPFQDLFTALEN
                     LHPGSLLILAGLNLVIILLLTSRWWLILRAQGHPVNLIELAGFRLAAFSIAYFTPGPQ
                     FGGEPLQVHLLRSRQGVSSSNAIASVSLDRLLELIANFSFLAVGLFILTQSGLLIALP
                     SGQALIFTLGLLLIPIIYLVSLGRYKKPLSAVLKRIHHPKWLAAGVLVEEAEELIGAF
                     CRQKPLTLLTTMFISLFTWIVMVGEYTLLLNFLGLAVSPAQAVIALTAARLAFIVPTP
                     GGLGAFEASQVLAAGALGFSPLSGLAVSLVIRARDLLFAAAGLWLSAHYLRRTPNYLR
                     VTQSIEEE"
     gene            complement(1819..>3002)
                     /locus_tag="VN363_03845"
     CDS             complement(1819..>3002)
                     /locus_tag="VN363_03845"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0000287 - magnesium ion binding [Evidence
                     IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWQ83672.1"
                     /translation="MELSTPYPSQSFFRLKLLWLRFGLISLVFLGAGTLALQVAWETR
                     FALRWLALASPALVYLIVVFRIGLHYNHRPEEVDLLPTLGAGNWMSLGRGVLLAALVG
                     FLFVPKPSGWLAWIPGVLYTLACAADFLDGYLARRTNHVTRLGEFLDMSFDGVGVFAA
                     ATLAVQYGQAPVWYLIIAAARYLFLFGLRLLKTLGKPVLPLHPSLMRRVFAGLQMGFL
                     AVILWPIMVPPASLFAATLFGIPFLFGFGQDWLVVSGLIKPRSGAGKLIAPFSTWISS
                     SLPVVLRGAVLALLVWISIQQFQQATLPVWLIALEWTAAIFVIFGTAGRIFATAGLIL
                     LGIGQIYTPVMLEQYSLAVLYTGLLFLGTGKYSLWAPEDQAVIGQAGKRHKPSEREQE
                     A"
BASE COUNT          799 a          827 c          815 g          561 t
ORIGIN      
        1 cgtaaacagc tggtgataaa ctctggctga ttgctgaagt ggtggagggc atgcacacag
       61 taaatcaggt caaagcttga ctccgaaaac ggaaacgctt ctgcctgacc ctgtaccagg
      121 taaaacccat gcttgcgcag agatgcctga cgcagcatcc caatcgatct atccaggccg
      181 aaaaattttt ggccagcctg tttcgcgaat gccacttcta atccagccag ccagaaacct
      241 gttccacagc cggcttccag aactgcccca gcgtggattt gccgggctaa actccgcagc
      301 gcatctaaga tcggctggga tttctctccc tggaagcggc agttattggt cggggcgatc
      361 tggtcgtagt tgactgctgt tctggtcatt aagaccaagt atatcagacc cgatcctcaa
      421 tctcacaacc cacccctggt ccatcgcgca gccctgtctg gttaccgccc gcggtccatc
      481 cacaatagag ccagtaaagc cacctcgata attttatcga tataaccgag tggggtacgc
      541 attccaatcg ctacccatgc gatgatggtg atgagagtga gggccatcaa ggcataccgg
      601 atccatgtgc ggtacgattt gaattggggt aggaacagcg ctactgctaa cacaacatag
      661 gcaatcccat tcaaatagaa catgattaat cggtccggga tcgccagcgt gaaatggatc
      721 aatccggttg ccaatgctaa gagcaggata cctgcctgaa tagggccgaa ggatgtttgt
      781 gccttttcca taattattcc tcctcgatcg attgagtgac acgtaaataa ttgggcgtac
      841 gcctcagata gtgtgctgac agccacaggc cagcagcggc gaatagcagg tcccgcgccc
      901 ggatcaccag gctgacagcc agaccgctca gcgggctaaa ccccagcgca ccggcagcca
      961 acacctggga agcctcgaac gcacccaggc cgcccggcgt cggtacaata aaggccaggc
     1021 gtgctgcggt gagggcaata acagcctggg ccggggaaac agccaggccc agaaaattca
     1081 gtaacaacgt atactcgccc accatcacta tccaggtaaa taacgatatg aacatcgtgg
     1141 tcaggagggt gagcggcttc tggcggcaga atgcgccgat cagctcttca gcctcttcca
     1201 ccaatacgcc tgccgctagc cattttggat ggtggatacg ctttaagact gccgagagag
     1261 gctttttgta cctcccaagc gaaaccagat agataatcgg gataagcaat aatcccagcg
     1321 taaaaatcag cgcctgacca gagggaagcg caatcaatag gcccgactgc gtgagaatga
     1381 acaggccaac cgccaggaag gaaaaattgg cgattaattc gagcagtctg tccaggctca
     1441 ccgaagcgat ggcgtttgag ctggatacac cctggcgaga gcgcaggagg tggacctgga
     1501 gcggctcgcc gccgaactgg gggccgggtg taaagtaagc gatgctaaaa gctgccagcc
     1561 ggaagccagc cagctcgata agatttaccg ggtgtccctg ggcgcgcagg atcagccacc
     1621 aacggctggt gagcaagagg atgataacca gattcagccc tgccaggatg agcagagagc
     1681 ctgggtgtag attctctaat gcggtgaata aatcctggaa cgggatactc ttgatagaca
     1741 ggatgaataa ccccagcgct gcggcccata ttccatacag ccaaaagggc tgtcttgacc
     1801 aggttctggc aaaagccttc atgcttcctg ctctcgctca gaaggtttat gccgcttacc
     1861 ggcctggcca atgacagcct ggtcttccgg cgcccacagc gaatactttc ctgttccaag
     1921 aaaaagcagc ccggtgtaaa gtacagccag gctgtactgt tctagcatga ctggcgtata
     1981 aatttgcccg atgccaagca gaatcaggcc cgccgtcgcg aagatccgcc cggctgtccc
     2041 aaagatcacg aagatggctg ccgtccactc cagggcgatc agccagactg gcagtgtggc
     2101 ttgttgaaac tgctggatag aaatccagac caggagggcg aggactgcac cacgcaggac
     2161 taccggaaga ctgctgctta tccaggtaga aaaaggagca attaattttc cagcacctga
     2221 ccggggtttt attaagccac tgaccaccaa ccaatcctga ccgaacccaa agagaaatgg
     2281 gattccaaaa agagtcgctg caaacagact ggcaggcggc accatgattg gccataagat
     2341 cactgcgaga aatcccatct gcagcccggc aaatacacgg cgcatgagag atgggtgcag
     2401 tggtaagaca ggtttgccca gggttttcag cagccttaat ccgaatagga ataaataacg
     2461 cgccgcagcg attatcagat accagactgg agcctggccg tactgcacag ccagagtagc
     2521 cgcagcaaag acacccacac catcgaagct catatccagg aattcgccca ggcgggtgac
     2581 atgattggtc cgccgggcca gatagccatc caggaaatcg gctgcgcagg ccagtgtgta
     2641 taacacgcct ggaatccagg ccagccagcc ggaaggtttg ggtacaaaga gaaagccaac
     2701 cagggcggct aacaagacgc cccgtcccag ggacatccaa ttcccagcgc ccagagtggg
     2761 cagcaaatct acttcttccg gacggtggtt gtagtgtagt ccaatccgga agacgacaat
     2821 cagataaacc agagccggac ttgccaatgc cagccagcga agtgcaaagc gcgtctccca
     2881 ggctacctgc agcgcgagcg ttcctgctcc caggaatacc aggctgatca ggccaaagcg
     2941 cagccagaga agcttaagcc ggaaaaatga ttgtgagggg taaggagtgc tgagttccat
     3001 ag
//
LOCUS       DATMXK010000206         3284 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_97162, whole genome shotgun sequence.
ACCESSION   DATMXK010000206 DATMXK010000000
VERSION     DATMXK010000206.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3284)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3284)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3284
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97162"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..292
                     /locus_tag="VN363_03850"
     CDS             <1..292
                     /locus_tag="VN363_03850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83673.1"
                     /translation="TFAFGLQHPERVRALAGLAGFLPEGSAELAVGQPLLDKPVFLAN
                     GTQDELVPVEKARLAVTLLERAGATVNYCEHDVGHKLNAACFRSLQIFFEDN"
     gene            complement(391..>3284)
                     /locus_tag="VN363_03855"
     CDS             complement(391..>3284)
                     /locus_tag="VN363_03855"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWQ83674.1"
                     /translation="ASAAATHDRALLNSLTDLTGGATTPPGDILGRSFTSAFGPAEIV
                     YAGDYGDPLCPIGAFDPGTFSGQIVICDRGIHARLDKAQSVLNGGAGGFVLANDSASG
                     NSLTADAYALPGVHITYADGVVLKAWVDDGGASHTGEIAGTTPDYDPVNGDILASFSS
                     RGPNTTFDVLKPDLTNPGVDIWAAYKTPDPAVPGADEYAFVSGTSMAAPHTAGAGALM
                     TALFPTWSSNAILSALMMTADRTVLKEDGVTPAGNFDMGAGRVDLNLAAKAGLVLDET
                     YTNFVNADPGLGGDVKTLNTPSLTNSQCLQSCSWTRTFTSVADADVEYTVSSSGDFVI
                     TPDTAVFTISPGATQAITFTAVVSTLTNDVWASGLVTLTPDDAALPVLNIPVSVVPTS
                     GVFPRLVTINTRRDVGSQAILGVESIEVTELTTQSFGLERAELTTASIPVDPTNDNPY
                     DDPTDGAFFILEDVPANAVRLIAETVFAEAADVDLFVGIDADADGLPEESELVCSSTT
                     PTATERCDLMEPEAGMYWILVQNWAASAEGAVDAITLSLGVVTTDEGNMSVVGPETAP
                     ELTPYDLTVFYDITESEAGDRWYGAFSLGSSAATPGDIGLIAVDVVRFSDDVVKIAPE
                     GAVPGETVTYQVVVEPNVTPADLTYTIIDTIPTGLSLDPTSIDINIGSYVVVGNTIIW
                     TVPMPVPFYDYALTTSADDPLCITPFGLPYVDLAGSGIPANAGISGDTFAFTAFGTGA
                     PFDYFGTPYTGMGFTDDGFALFDVPTNYDGFPWFAQTVPDPAFPNNVLGMLWQDLEVI
                     YDAPTNRGVSLATAAGGALVVVEYDDVQLFEDPTNQYDFEVFAWRDPDNSPGAYEFVF
                     AYDNLDGTLAGPLTVGLENADATLGTALVNNGDATGVLSNGFNVCLDRITVGEAAVMT
                     YDVVVTADLGETILNTVTSGVDNPGSEATQAGADLFIGIPLYLPLLSKD"
BASE COUNT          707 a          919 c         1047 g          611 t
ORIGIN      
        1 gacatttgca ttcggactgc agcatcccga gcgggtgcga gctctggcag ggctggcggg
       61 attcctgcct gagggcagcg ccgagctggc agtcgggcag ccgctgctgg ataagccggt
      121 cttcctggca aacgggacgc aggatgagct ggtgcctgtg gaaaaagccc gcctggcagt
      181 tacgctcctg gagcgagcgg gggcgacggt aaactactgc gagcacgatg tgggccacaa
      241 gctcaatgca gcctgctttc gcagcctgca aatatttttc gaggataatt aaagggacag
      301 cgccgggaaa aaacccggcg ctgttgtttg taaggcgtct gagccagcca tccggttatg
      361 ccggaagctg gaacaggtac ccggtttaga ttaatccttg ctcaaaagcg gcaggtacag
      421 cgggataccg atgaacagat cggcgccggc ctgggtcgct tcactgccgg ggttatccac
      481 gccgctggtg accgtgttga gaatcgtctc gcccagatcg gcggtaacga cgacgtcata
      541 ggtcatcacg gcagcctcac ccacggtgat gcggtcaagg cagacattaa agccattcga
      601 cagaacaccg gtagcatcgc cattgttgac cagggcggtc cccagggttg catcggcatt
      661 ctccaggcct acggtcagcg ggccagcaag ggtgccgtcc aggttgtcgt aagcgaagac
      721 gaattcgtat gcacctgggc tgttatcggg atcgcgccag gcaaagacct cgaagtcata
      781 ctggttggtt ggatcctcaa acaactggac atcatcgtac tcgaccacaa ccaaagcacc
      841 gccagcggct gtcgccaggc tcacgccgcg gttggtcgga gcatcgtaga tgacctccag
      901 atcctgccag agcattccca ggacattgtt ggggaacgct ggatcgggca cagtctgagc
      961 gaaccacgga aagccatcgt agttcgttgg gacatcgaag agggcaaagc cgtcatccgt
     1021 aaagcccata cccgtataag gtgtgccgaa gtaatcgaag ggtgctccgg ttccgaatgc
     1081 ggtgaaagca aacgtatcgc cagagatgcc agcattcgcg gggatgccag agcctgccag
     1141 atccacgtac ggcaatccaa acggggtaat gcacagcgga tcgtctgcgc tggtggtcag
     1201 agcatagtca tagaacggca cgggcattgg cacggtccag atgatcgtat tgcccacgac
     1261 aacataggag ccaatattga tatcgatgga ggttggatcc aggctgagcc cggtggggat
     1321 ggtgtcgatg atcgtgtaag tcaggtccgc gggggtgacg ttgggttcaa cgaccacttg
     1381 gtaggtgacc gtctctccgg ggacggcccc ttccggagca atcttaacca catcgtcgct
     1441 gaagcgaacc acatccaccg cgatcaggcc gatatcaccc ggggtggctg cgctggaacc
     1501 gagcgagaaa gctccgtacc agcgatcgcc ggcttcggat tcggtgatat cgtaaaagac
     1561 ggtcaggtca tagggggtga gctcgggggc tgtctctggt ccaaccacac tcatattccc
     1621 ctcatcggtg gtaaccacgc ccaaggacag ggtgatggca tcgactgcgc cctccgccga
     1681 agctgcccag ttctgaacca ggatccagta catgccagct tcaggctcca tcaggtcgca
     1741 gcgttcggta gcggtcgggg tggtgctgga gcagaccagc tcgctttctt ccggcagtcc
     1801 atctgcgtca gcgtcgatgc caacaaacag atcgacatcc gctgcttcag caaagaccgt
     1861 ttcggcgatc aggcggaccg cgttggcggg gacatcttcc aggatgaaga aagcgccatc
     1921 ggttggatca tcataagggt tgtcgttggt cgggtcgacc gggatggaag cggtggtcag
     1981 ctccgcccgc tcgaggccga aactctgcgt ggtcagctcg gtgacttcga tcgactcgac
     2041 gcccaggatg gcctgtgagc caacatcgcg gcgggtattg atcgtcacca acctggggaa
     2101 cacgcctgag gtcggaacaa ccgagaccgg gatgttgagc accgggagag ccgcatcatc
     2161 aggggtcagc gtgaccaggc cagatgccca gacatcattg gtcaacgtgc tgacgacggc
     2221 tgtgaaggtg atcgcctggg ttgctccagg agagatggtg aagacggctg tgtctggcgt
     2281 gatgacgaaa tcaccgctcg aagatacggt atattccaca tccgcatccg ctaccgaggt
     2341 gaaggtgcgc gtccagctgc agctctgcaa gcactggctg tttgtcaggc tgggcgtatt
     2401 gagggtcttg acatccccac ccagaccggg atcggcattg acgaaattgg tataggtctc
     2461 atccagaacc aggccggctt tggcagccag gttcaaatca acccgcccgg cgcccatatc
     2521 aaaattcccg gccggggtga cgccatcctc tttcagcacc gtacggtccg cggtcatcat
     2581 cagggccgaa aggatcgcat tggagctcca ggtggggaag agggcggtca tcaatgcgcc
     2641 agcgccggcg gtatgcggcg cagccattga ggtgcctgaa acaaacgcat attcatcggc
     2701 tccaggaacg gctggatcgg gagtcttgta cgccgcccag atatcgacac ccgggttggt
     2761 caggtcgggc ttgagcacat cgaaggtcgt attggggccg cgcgagctga aagaagccag
     2821 gatatcccca ttcaccggat cataatccgg ggttgtaccg gcaatctcac cggtatggga
     2881 tgcgccgcca tcgtccaccc aggctttgag gacgacacca tctgcgtagg tgatatgcac
     2941 gccggggaga gcgtaagcat cggccgtgag cgagttgccg ctggcagaat cattcgccag
     3001 cacaaatcca cctgcgccgc cgttgaggac gctctgggct ttatcaagcc gggcgtggat
     3061 gccgcgatcg cagatcacaa tctgaccgct aaatgttccg gggtcaaagg caccgatcgg
     3121 gcagagcgga tcgccgtagt cgccggcgta gacaatctca gccgggccaa aggcagacgt
     3181 gaagctgcgc ccgaggatat cgccaggggg cgtggtggcg ccgccggtca ggtcggtcag
     3241 gctgttgagt aaggcgcggt cgtgcgtggc agctgccgaa gcag
//
LOCUS       DATMXK010000207         2521 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_17922, whole genome shotgun sequence.
ACCESSION   DATMXK010000207 DATMXK010000000
VERSION     DATMXK010000207.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2521)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2521)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2521
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17922"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..897)
                     /locus_tag="VN363_03860"
     CDS             complement(<1..897)
                     /locus_tag="VN363_03860"
                     /EC_number="2.5.1.19"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003866 - 3-phosphoshikimate
                     1-carboxyvinyltransferase activity [Evidence IEA];
                     GO_process: GO:0008652 - amino acid biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoshikimate 1-carboxyvinyltransferase"
                     /protein_id="HWQ83675.1"
                     /translation="MNLTVFPGHPLRGTVHLPGDKSLSHRAALFAALAEGESRIENFL
                     NAGVTRAMLNALTMLGIEWEMDGRDTLRISGHGLIGFTAPSHMLDCGNSATTLRLLTG
                     ALAGSGRAAALDGTPGLRRRPMHRIVHPLQQMGVPIQATLEGTAPLQLASPSDNHPLR
                     GMRHTLNVASAQVKTCLLLAGLGASEPTETAEPAASRDHSERMLRRMGVSVEQFVMSD
                     KCLTRVTPPENPLEPLQLRLPGDISSAAFFIVAALITPGSEITIPDILLNPGRTGLIE
                     VLQSMGAQIEVQADSNLEWEPCG"
     gene            complement(894..1775)
                     /locus_tag="VN363_03865"
     CDS             complement(894..1775)
                     /locus_tag="VN363_03865"
                     /inference="COORDINATES: protein motif:HMM:NF014234.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004665 - prephenate dehydrogenase (NADP+)
                     activity [Evidence IEA];
                     GO_function: GO:0008977 - prephenate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA];
                     GO_process: GO:0006571 - tyrosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prephenate dehydrogenase/arogenate dehydrogenase
                     family protein"
                     /protein_id="HWQ83676.1"
                     /translation="MSDPAGNPLSEARIAILGLGLMGGSLAYDLAGKCAALVGVDPDL
                     ETQNLARASGCFQAVYARPTEIYSSVDVWILAAPVRAILTILAELPGWDSRSGIVLDI
                     GSTKTQILAAMEELPVRFDPLGGHPMCGKETGSYASAGPGLFQGAAFAWCRLERTSPE
                     AIQLATALTAAIGGIPRWLSAADHDRWTAATSHLPYLIASALAGTTPGDAAPLIGPGF
                     QSTTRIAGSPPEMMLDVLLTNRQQILAAAARFQAGFQGFIDLLQSGEKAEIEQLSAHL
                     QAGRKSRQHLLDSVNKP"
     gene            complement(1768..>2521)
                     /gene="aroF"
                     /locus_tag="VN363_03870"
     CDS             complement(1768..>2521)
                     /gene="aroF"
                     /locus_tag="VN363_03870"
                     /EC_number="2.5.1.54"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003849 - 3-deoxy-7-phosphoheptulonate
                     synthase activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="3-deoxy-7-phosphoheptulonate synthase"
                     /protein_id="HWQ83677.1"
                     /translation="FETAFAVKEAGAHALRGGAYKPRSSPYSFQGMGEAGLELLAEAR
                     QLTGLPIVSEVMAQDQIPLMVRYVDVLQIGARNMQNFSLLHAVGESHHPVLLKRGLMA
                     TIEELLMAAEYILSHGNQRVVLCERGIRTFETATRNTTDINAIPVLKSLTHLPVILDP
                     SHSTGHWEYVTSIARAGIAAGADGLIVEVHPNPSEAFSDGGQSLKPERFGELVRQCRA
                     VAEAVGRKVAPPSSNQPVPELAPSPAEPRQHE"
BASE COUNT          497 a          732 c          805 g          487 t
ORIGIN      
        1 cccgcagggt tcccactcca ggttgctatc tgcctgcacc tcgatctgcg ctcccatgct
       61 ctgcaaaact tcaatcagcc cggttcggcc tgggttgagc agtatatccg ggatggtgat
      121 ctccgaaccc ggggtgatca aagcggcgac gatgaagaag gctgcgctgg agatatcccc
      181 gggcagtctg agctggagtg gttccagggg gttttcgggt ggggtaaccc gggtcaggca
      241 cttatcactc ataacaaact gttcgacgct cacgcccata cggcgcagca tccgctccga
      301 atggtcgcgc gaagcagccg gctcggcggt ctccgtcggc tcgctggcgc ccaacccggc
      361 caggagcagg caggtcttta cctgggcgct ggcgacgttc agcgtatggc gcatcccccg
      421 cagcgggtga ttgtccgaag gcgaggcgag ctgtagagga gcggttccct ccagggttgc
      481 ttgaatggga acccccatct gctggagtgg atgcacaatc cggtgcatcg ggcggcggcg
      541 caggccaggt gtcccatcca gggctgcggc tcttccagag ccagccagcg ccccggtgag
      601 cagccgcagg gtggtagccg aattgccgca gtccagcata tgggagggag cggtaaagcc
      661 aatcaggccg tgtccagaaa tccttaaggt atctctgccg tccatttccc actctatccc
      721 cagcatagtg agagcattga gcatcgcccg ggtaacgcca gcatttaaga aattctcgat
      781 ccggctttca ccttccgcca gagcggcgaa caatgctgcc cggtgggaga gggatttatc
      841 tccggggagg tggacagttc cacgcagtgg gtgacctggg aagaccgtga ggttcatggt
      901 ttatttactg agtcgagcaa atgctggcgg cttttccgac cagcctgaag gtgggcgctc
      961 agctgttcaa tctcagcctt ctcaccgctt tgcagcaggt cgatgaaacc ttgaaagcca
     1021 gcctgaaaac gggctgctgc agccaggatc tgctggcggt tggtgagcag aacatccagc
     1081 atcatttctg ggggcgaacc agcgatgcgg gttgtgcttt ggaagccagg accgatcagg
     1141 ggcgcagcat cgccgggggt tgtaccggcc agagcgctgg cgatcagata aggtaaatgg
     1201 ctggtcgcgg ctgtccagcg gtcatggtct gcggcggaaa gccagcgtgg gattccgccg
     1261 atcgcagcag tcagtgcggt ggccagctgg attgcttccg gagacgtacg ctccaggcgg
     1321 caccaggcga aggctgcgcc ctggaataaa cccggcccgg cgctggcgta gctgccggtc
     1381 tcctttccgc acatcggatg gcctccgagc gggtcgaagc ggacaggtaa ttcttccatg
     1441 gcagcgagta tctgggtctt ggtagaaccg atatcgagca cgattccgct tcgggaatcc
     1501 cagccaggca gttccgccag aatcgtcagg atagcacgca ccggcgctgc caggatccaa
     1561 acatccaccg atgagtatat ctcagtggga cgggcgtaga cggcttgaaa gcagccgctg
     1621 gcgcgggcta aattctgggt ttcgagatcc ggatcgaccc caaccagcgc tgcgcacttg
     1681 ccggcaagat catatgccag ggatccgccc atcaggccca gtcccaggat ggcgatgcgg
     1741 gcttcgctca gtggattgcc ggctggatca ctcatgctgg cggggttcag cagggctggg
     1801 cgccagctcg ggaaccggct ggttgctgga tgggggtgct accttgcggc ccactgcttc
     1861 agcgacagcc cggcactgcc ggacgagctc accaaagcgc tccggcttta aggactgccc
     1921 cccatcggaa aaagcttcac tgggattagg atggacctca acaatcaggc cgtcagcacc
     1981 ggcggcgatc ccggcgcggg cgatcgaggt tacatattcc cagtgtccgg tggaatggct
     2041 ggggtcgagg atcactggca aatgggtcag ggatttgagc accgggatag cgttgatatc
     2101 ggttgtattc cgggtagccg tctcaaatgt gcggataccc cgctcgcata atacgactcg
     2161 ctggttgcca tgcgaaagga tatattccgc agccatcaag agctcttcga tagtcgccat
     2221 cagaccccgt ttgagcagaa ccgggtggtg gctctcaccg acggcgtgca gcagcgaaaa
     2281 attctgcatg ttgcgagcgc cgatctggag cacatccaca taacgaacca tcagggggat
     2341 ctggtcctgg gccatcacct cgctcacaat tggcagaccg gtgagctggc gcgcctctgc
     2401 cagaagctcc aatccagctt cgcccatgcc ctggaaagaa tacggtgagc tgcgcggttt
     2461 gtaggctcca ccgcgcaagg catgggctcc ggcttctttg acggcaaagg cggtttcaaa
     2521 g
//
LOCUS       DATMXK010000208         2529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_123928, whole genome shotgun sequence.
ACCESSION   DATMXK010000208 DATMXK010000000
VERSION     DATMXK010000208.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_123928"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(13..672)
                     /locus_tag="VN363_03875"
     CDS             complement(13..672)
                     /locus_tag="VN363_03875"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560689.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006813 - potassium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-binding protein"
                     /protein_id="HWQ83678.1"
                     /translation="MRVIVVGCGRVGSELAFRLFEKGHKVTVVDTLHSAFENLDPRFR
                     GRTIEGEALNQEVLQRAGIEQADGIAVVTNSDTLNTVIAHVAKSVFQVPNVVARNYDP
                     NWRNLHEVFDLQVVSTSSWGAQRIEELLYHQEMRTVFSAGNGEVEIYEFTVPEALNGK
                     TLGDLIGTHECIPVAITRSGKAILPGIDSVVHAGDAVLVSATFLGTQNIRNLLGSLEE
                     A"
     gene            complement(708..>2529)
                     /locus_tag="VN363_03880"
     CDS             complement(708..>2529)
                     /locus_tag="VN363_03880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560690.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWQ83679.1"
                     /translation="QTIGKAVGLAVFASDALSSTAYATQEILVILAVAGSGAFGYAFP
                     ISLAIVVLMGIVTISYEQTIHAYSGGGGAYIVSRDNLGELPAQMAGAALLTDYILTVA
                     VSISSGVAQITSASPMLAEYKVFIGVGLIFFVMLINLRGVRESGVIFAVPTYFFILMM
                     FVMLLVGFIKFTTGTLGMVVNPPSLEVFGPFVAVTPFLILHAFSSGTSAMTGIEAISN
                     GITAFKEPRSRNAGITLIAMALILGTLFLGITFLTGEIHAIPSEHETVISQLARTIYG
                     DRGIFYLMTIAATSVILIMAANTAFADFPRLSALLAQDGFLPRQYAFRGSRLVFSNGI
                     VSLAIFASILLIIFKASVTALIPLYAIGVFLSFTLSQTGMARRWNKIGHLKPNEEIVE
                     RGSTLKYDRRWTLKMIINGFGAFLTLIVMVVFAYTKFADGAWVVIIIIPLVVYVFASI
                     HRHYRNLAKSLSLEDFGESPITPRHRVILPISGVHRGTLLALRYAQTLSNDVTAVHVA
                     IDPAETEKLEKKWENWGEGVRLVTLNSPYRLLLEPLIDYIEEVASQRQPNDTITVVVP
                     QFVPKKWYHNILHMQTAFLLRSALIFKPGIMITDVPYQVD"
BASE COUNT          739 a          633 c          557 g          600 t
ORIGIN      
        1 attacaaaca agtcaggcct cctctagcga gcccaataaa tttcgaatat tttgagtgcc
       61 cagaaatgta gcgctaacca gaactgcatc ccctgcgtgg acgactgaat caatgccagg
      121 taagatagct ttaccagaac gggttatcgc gaccggaata cactcatgag taccgatcag
      181 atcaccgagc gttttgccat tcaaggcttc aggaacggta aattcataga tttctacttc
      241 accatttcct gcagaaaaaa ccgtccgcat ttcctgatgg tagagcagtt cctcaattcg
      301 ctgagctccc cagctgctgg tgctgacaac ttgtagatcg aatacttcat gcaggttacg
      361 ccagttggga tcataattcc gagcgacaac atttggaacc tgaaagactg attttgcgac
      421 gtgggcaata acggtattga gagtgtcgga gttggtgaca actgcgattc catccgcttg
      481 ttctattccg gcgcgctgga ggacttcctg gttgagagcc tcaccctcga ttgtccgccc
      541 tctaaatcta gggtccagat tctcaaatgc actatgcagg gtgtcgacga ccgtaacttt
      601 atgccctttt tcaaacagcc ggaaggctaa ctccgaacca acccgaccgc agccaacaac
      661 gatgactctc ataatctcag ctccactcac tcatcaattc atccagatta atcaacctgg
      721 taaggcacat ctgtgatcat tatgccaggt ttaaaaatca atgcagatcg cagcagaaaa
      781 gctgtctgca tatgtaatat attatggtac cacttcttgg gaacgaattg tgggaccaca
      841 acagtgatgg tatcgttcgg ttggcgctgg ctggcaactt cctcgatgta atcgatcaat
      901 ggctccagga gcaagcgata gggcgaattg agggtcacca ggcgtactcc ttcgccccaa
      961 ttctcccatt tcttctccag cttttcagtt tcggctggat cgatcgccac atgaactgca
     1021 gtaacatcat tcgataatgt ttgcgcataa cgcaaagcta acagcgttcc acgatgcacc
     1081 cctgagatag gcagaattac gcggtggcga ggtgtaatcg gggattctcc aaaatcttcc
     1141 agggataagc ttttggccag gtttcggtag tgacgatgga ttgaggcaaa tacatagaca
     1201 accagcggaa tgataataat tacaacccat gccccatccg caaattttgt ataggcaaaa
     1261 accaccatta cgatcaatgt caggaaagcg ccaaaaccat tgattatcat cttcaatgtc
     1321 catcgcctgt catacttaag agttgatcca cgctcgacaa tttcctcatt gggcttgaga
     1381 tgcccaatct tattccagcg cctggccata cctgtctgtg aaagcgtaaa cgatagaaat
     1441 acaccgatcg catacaatgg aattaaggct gtcacactgg ccttaaaaat tatcaaaagg
     1501 atgctggcaa atatggccag ggagacaatc ccgttcgaaa agaccaatcg gctgccgcgg
     1561 aaagcgtact ggcgcggcaa aaaaccatct tgagctaaga gagcactcaa gcgaggaaaa
     1621 tcagcaaagg ctgtgttcgc tgccataatc aagattacac tggtcgcggc gattgtcatc
     1681 aaatagaaaa taccacgatc accataaatc gtgcgcgcta attgagaaat taccgtctca
     1741 tgctctgaag ggatcgcatg gatttcacct gttaaaaagg tgatacccag gaacaatgta
     1801 cccaatatca aggccatcgc aattaaagtg atccccgcat tacggctgcg tggctctttg
     1861 aatgctgtaa tcccattcga aatcgcctcg ataccggtca tggcggatgt accgctcgaa
     1921 aatgcatgca agatcaagaa tggtgtcaca gcaacaaatg gaccgaatac ttctagagaa
     1981 ggcggattga cgaccattcc cagtgtaccc gttgtaaatt tgataaagcc caccagcagc
     2041 ataacaaaca tcatcaaaat gaaaaaatag gttggaacgg caaatattac gcctgattct
     2101 cgcactccac gcaaatttat cagcattacg aagaaaatta atcctacgcc gatgaaaacc
     2161 ttatactcag ccaacatcgg cgaggctgac gttatctggg ctacacctga tgaaatcgaa
     2221 actgctacgg ttaagatgta atcggtcaat aaagcagctc ctgccatttg ggcaggcaat
     2281 tcacccagat tatcccttga gacaatatat gccccccctc cacctgagta ggcatggatt
     2341 gtttgctcat aagagattgt gacaattccc atcagaacga caatcgccag cgagatcgga
     2401 aaggcatagc caaatgcacc actgcccgca actgccagaa ttaccaggat ttcttgagtt
     2461 gcataggctg tagatgagag ggcgtccgac gcaaatacag ccaggccaac tgctttaccg
     2521 atagtttgg
//
LOCUS       DATMXK010000209         3062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_139350, whole genome shotgun sequence.
ACCESSION   DATMXK010000209 DATMXK010000000
VERSION     DATMXK010000209.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139350"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..2377
                     /locus_tag="VN363_03885"
     CDS             137..2377
                     /locus_tag="VN363_03885"
                     /EC_number="2.7.6.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560073.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0015970 - guanosine tetraphosphate
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bifunctional (p)ppGpp
                     synthetase/guanosine-3',5'-bis(diphosphate)
                     3'-pyrophosphohydrolase"
                     /protein_id="HWQ83680.1"
                     /translation="MNIENLIEALPTTYSTLDRELVRRAYKVAETAHTGQKRATGEPY
                     ITHCLAVAAILAEMRVPPSVIAAGLLHDTVEDTPVTLDDIRRDFGPEITTLVDGVTKL
                     TQLPRVSRADQLKVTAGDPEEQPLTSERRFLPDPDEEAERIMRSRRYDVVSETLRKTF
                     MAMGDDVRVVLIKLADRLHNMRTLSHIPETKQRRIAQETLDIFAPLANRLGIWQLKWE
                     LEDLAFRYVNPETYREIADKIAQRRSDREHQIKDIAENLHKMLAEAGIQAEVTARPKH
                     IYSIYKKMVRKGVPFDMVHDIRGVRILLKDIPSCYAALGVIHTRWRPIPGEFDDYIAA
                     PKDNFYRSLHTAVIYEDGMNLEVQLRTQEMNQNAEYGIAAHWRYKEGAARDDDYERRI
                     LWLRSLMEWRQDVDDAREFVDSMKTDVFEDRVYVFTPRGDIIDLPSGSTPIDFAYHVH
                     TDIGHRCRGAKINGKLTTLDYQLKTGDKVEVLTAKRGGPSRDWLNQNLGLVKTQRARA
                     KIRQWFKKQAFEKNASQGKTILEKELRRLGLQDVNQERLAREFDYKLVEDLFEAIGTG
                     DLTLSRVVNHLTLGEKPDPEFTLTASKIDGSGPSSDTVTVVGLKGLLTSMARCCNPAP
                     GDPIVGYVTRGRGATIHRQDCPNILRIRDRERLVRVSWGEPKNTYPIPVRIKAYDRNG
                     LMKDVSSLISDEGVNMPKVTVEVNRNMAVFDLILEVRDIVQLSRVLDRLENLDNVLEA
                     QRVRPG"
     gene            2556..>3062
                     /locus_tag="VN363_03890"
     CDS             2556..>3062
                     /locus_tag="VN363_03890"
                     /inference="COORDINATES: protein motif:HMM:NF015418.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83681.1"
                     /translation="MVAYPDSQPLLSVAAARTRLLDRFSKQPVEHLKLTQSGGRVLAE
                     TIYAGYDLPPFSNSSMDGFAVRAADIEQARPEAPVHLRVVADIPAGTEIEQIVRSGEA
                     IRIMTGAPLPSGADTVVPVENTDFNERSAGTLAPEAVAVYRPEKVGENTRPRGEDVRA
                     GEAVLHAGS"
BASE COUNT          696 a          830 c          898 g          638 t
ORIGIN      
        1 catttgaacg aatttgcctc ctactttggt ttgattaata atctcctgat tttgataccg
       61 ccgttcagcc gtaatctggt tcacccttgt accagccggg tgcactgata tacttaatac
      121 ggagctgttg ttaaggatga atatcgagaa tttgatcgag gcactcccaa ctacatattc
      181 gacgctggat cgtgagctcg tccgccgcgc ttacaaggtg gctgagactg cgcacaccgg
      241 gcagaagcgc gccacaggcg agccttatat aacccactgc ctggctgttg cggcgatcct
      301 ggctgagatg cgcgtgcctc cttccgtgat tgctgctgga ctgcttcatg ataccgtcga
      361 ggatactccg gtgaccttag acgatatccg gcgcgatttt ggtcccgaaa ttaccaccct
      421 ggtcgatggc gtgaccaaat tgacccagct gccgcgggtt tctcgggctg accagctgaa
      481 agtaactgcc ggcgaccctg aggaacagcc cctgaccagc gagcggcgct tcctgcctga
      541 tcctgacgag gaagcggagc gcatcatgcg cagccggcgc tacgatgtgg tttccgaaac
      601 cttacgtaaa acctttatgg cgatgggcga tgatgtgcgg gtcgtgttga tcaaactggc
      661 cgaccgcctc cacaatatgc gcaccctgag ccatatcccg gaaaccaagc agcgccggat
      721 cgcccaggag accctggata tctttgctcc cctggcgaac cgtctcggca tctggcagct
      781 gaaatgggag ctggaggacc tggcattccg gtatgtgaat ccagagacct accgcgagat
      841 tgcggataag atcgcccagc ggcggtctga tcgcgagcac cagatcaaag atatcgccga
      901 aaatctgcac aaaatgctgg cagaggcggg gatccaggct gaagtcaccg cgcgcccgaa
      961 acacatctac tcgatctata aaaagatggt gcgcaagggc gttccatttg atatggtgca
     1021 tgatattcgc ggagtgcgca tcctgctaaa ggatatccca tcctgctatg cagcgctggg
     1081 ggtgatccac actcgctggc gccccatccc cggtgaattt gacgattata ttgctgcccc
     1141 aaaagataac ttttaccgct ccctgcatac ggcggtgatc tatgaagacg gaatgaacct
     1201 ggaggtgcag ctgcgcaccc aggagatgaa tcagaatgcc gaatatggga tcgctgccca
     1261 ctggcgttac aaggaaggcg cggctcgcga tgatgactat gagcggcgga tcctgtggct
     1321 gcgctccctg atggagtggc ggcaggacgt ggacgacgcc cgcgagttcg tggacagcat
     1381 gaagacggac gtatttgaag atcgcgtcta tgtctttaca ccccgcggcg atattatcga
     1441 tctgccgtca ggctcgacac cgatcgattt cgcttaccat gtgcacacgg atattggaca
     1501 ccgctgccgg ggtgcgaaga tcaacgggaa gctgaccacg ctggactacc agctcaagac
     1561 cggcgataaa gtggaagtgc tgactgccaa gcgcggcggg ccgagccgcg actggcttaa
     1621 ccagaacctg gggctggtga aaacccaacg cgcccgggcg aagattcgcc agtggttcaa
     1681 aaagcaggcg ttcgagaaaa acgccagcca gggtaaaacg atccttgaaa aagagctccg
     1741 ccggctgggt ttacaggatg tcaaccagga gcgcctggcg cgtgaatttg actataagct
     1801 ggtcgaggac ctgtttgagg ccatcggcac cggcgacctg accctgtcgc gggtggtcaa
     1861 ccatctgacc ctgggtgaga aaccagatcc agaattcacc ctgacggctt cgaagattga
     1921 cggcagcggt ccctccagcg acacggtgac ggtggtcggt ctgaaaggac tgctcacgag
     1981 tatggcacgc tgctgcaacc ctgcgcctgg agaccccatc gtgggctatg tcacgcgtgg
     2041 ccggggggcg accatccacc gccaggactg cccgaatatc ctgcgcatcc gtgatcgcga
     2101 gcggctggtg cgggtttcgt ggggtgagcc taagaatact tatccgatcc cggtgcgtat
     2161 caaagcttac gaccggaatg gattaatgaa agatgtctcc agccttatct cggatgaagg
     2221 cgttaacatg ccgaaggtga cagtagaagt aaaccgtaac atggcagttt tcgatttgat
     2281 cctggaagta cgcgatatcg tccagctcag ccgggtgctg gacagactgg aaaaccttga
     2341 taacgttctc gaagcccagc gagtccggcc tggttagcgt ttaacccgga cggagacttg
     2401 ccctgggttt attaaattca gggggaaatt gagcttgaca aatccgtgac agtggtataa
     2461 ttccgcgccg atgagccgga gtaacgcaaa ctaaatcctg ccagcagtca ggcttttagg
     2521 gcggttcgtg ttaaacaaga gaatggagag actgcttggt cgcttaccct gattcgcagc
     2581 cacttctatc ggtcgcagca gcccggacgc ggctattgga ccgattttca aaacaaccgg
     2641 ttgagcactt gaaattaacc cagtctggtg ggcgggtgct ggctgaaacg atctacgcgg
     2701 gttatgatct ccccccattt tcaaattcaa gtatggatgg ttttgcggtg cgggcagccg
     2761 atattgagca ggcgcgtcct gaagcacctg tccacctgcg cgtggtcgcg gatattccag
     2821 ccgggacgga aattgaacag atcgtccgct cgggtgaagc gatccgcatt atgaccggag
     2881 cacccctgcc atctggcgcg gataccgttg tgccggttga aaatactgat tttaacgaac
     2941 gctcggcggg aacgcttgcc ccggaggctg tagctgtata cagaccggaa aaagtaggcg
     3001 aaaatacccg cccccggggt gaagatgtgc gggccggtga ggctgtactc cacgctggca
     3061 gc
//
LOCUS       DATMXK010000210         5561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_125924, whole genome shotgun sequence.
ACCESSION   DATMXK010000210 DATMXK010000000
VERSION     DATMXK010000210.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5561
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125924"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..484)
                     /locus_tag="VN363_03895"
     CDS             complement(<1..484)
                     /locus_tag="VN363_03895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83682.1"
                     /translation="MPTESEPSGVPAAEPPQARPHSAGQGTPSLWASAPEARRPLPGW
                     LPLALGGLLIVTGLLLSLIPWGTVVPWLSVTLHSMLAADYSPDLSGARLPPVRLALAA
                     EALADQGYLQTAAAPGLGLSATPGDILATLQTGLLTPIPTVTPRPGETLFPLITLTPS
                     P"
     gene            complement(474..2801)
                     /locus_tag="VN363_03900"
     CDS             complement(474..2801)
                     /locus_tag="VN363_03900"
                     /inference="COORDINATES: protein motif:HMM:NF026177.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWQ83683.1"
                     /translation="MPRRVLVFQSDPLQAKNLSRYFLNQGDRVWKTARLSEAVALLDS
                     EPPELALIDLDAPDGAGLALIRQCRRRAPMAQLVITNRQVDLRAEIQARELGAAIFLR
                     EPFTPIWIERALRQASPARLPPVTPVPGERAIPRVGIPMRFKITVPYVLLALTFTLMA
                     AFLIGRYIQESLQERFTNQLADTGRLAADWMVAEEQRRLETLRLIANTEGVSEAIEAG
                     DAERLRSLVLPLAINAGEEAVAVLDRTGTSLIGLHRNPEDPPGEYRATRNSRLLAEQP
                     FVQRVLLGDVDILGDKFAGLAPGDSEQIFYIAGPVRDLNNQPVGVVLVGKTLPSLARQ
                     LRQSTLGHITFYTPDGAPLASTLFEPTRVQPLDPADSLGVLLNQDQSSFQRDIQSASS
                     TYTEILGPWEARSGADLGIMGIALAPNLLAAPRQATGVQVMVFIGLIFLAVILVGLLT
                     ARMITLPLKQVVDASRRVAEGNFQIKVKPSGNDEISVLATTFNYMVAGLQEGSIYRDL
                     LGRTVSPQVRDALRESFASGELRLEGQSTVGTVLISDIRNFTNLAEKEDAPTILKWLN
                     EYFGRMVPVVNQFGGVIDKFEGDSMMAFFGILPTRLPSEQSSFQACQAAVALMQVVNE
                     YNAERSLRGDPPMITGFGINTGMLMVGGLGATDRLDYTIIGDTVNTAQRIQELTRQFG
                     ETGCVVGENTLSALGEQRLDFRFDPLGEHALKGKSEVLWVYRMRPGDSAARLRPDSQR
                     AADAASQAAAPDLGAGSPAVQPPPAPRVQDAGHAD"
     gene            complement(3073..3909)
                     /locus_tag="VN363_03905"
     CDS             complement(3073..3909)
                     /locus_tag="VN363_03905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558849.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006865 - amino acid transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter permease"
                     /protein_id="HWQ83684.1"
                     /translation="MAKSTPSALTADEKALAYVPGAEPSARIDAWWGLVLLVVVLIIV
                     MVVGQPDPYRRILLFVRDGLWVTIYITALSFLFTLIAGLIGGLGRLSKNRLVFAVSTL
                     YVELIRGIPLMVQLIWWYFAFPAILKSFGQARGIPALANYSANANAMAIIGLTICYGA
                     YMSEIYRAGIQSIPNGQMEAARSLGMTYFQSMRFVILPQAVRVVLPPVGNEFVAMLKD
                     SSLVSVVAVADLTRRGREFMSVNFNPIEVWSLVALIYLVMTLFSARVVSGIEKRTRAS
                     SH"
     gene            complement(4112..4864)
                     /locus_tag="VN363_03910"
     CDS             complement(4112..4864)
                     /locus_tag="VN363_03910"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="basic amino acid ABC transporter
                     substrate-binding protein"
                     /protein_id="HWQ83685.1"
                     /translation="MKSRSWILMALVMLLSLILAACSSGGGEKVTVATDATWPPFEIV
                     DETTKEIIGFDIDLMKAIAEKGGFEVEFVNVGFDPLLAGMASCQYDAAISAITITEER
                     KADFAFSGPYFSAGQVVAVRADNSDIAGKDSLSGKTVGAQIGTTGAIVIEEIAGATLK
                     TYDDIGLAYQDLINGQVDAVVADDGLARGYVSKNSAELKIVGDPFTDESYGIAVCKTN
                     ADLLAKINTGLEAVRAEGLLETLTQKWLVGEG"
     gene            complement(5126..5207)
                     /locus_tag="VN363_03915"
     tRNA            complement(5126..5207)
                     /locus_tag="VN363_03915"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(5171..5173),aa:Leu,seq:gag)
     gene            5343..>5561
                     /locus_tag="VN363_03920"
     CDS             5343..>5561
                     /locus_tag="VN363_03920"
                     /inference="COORDINATES: protein motif:HMM:NF014554.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83686.1"
                     /translation="MVRIALDAMGSDDCPEPEIQAAVQAGRLYPDEIFLVGPQDRLEA
                     RLQALGGKHPRVRIIHAPETITMEDKGLK"
BASE COUNT         1238 a         1605 c         1641 g         1077 t
ORIGIN      
        1 ggggggacgg cgtaagggtg atcagtggga aaagggtttc gccgggacgt ggggtgacgg
       61 ttggaatcgg ggtcagcagg ccggtttgca gggtcgccag gatatcgcca ggtgttgcgc
      121 ttaaccccag acccggggca gctgcggttt ggagataacc ctgatccgcc agggcttcgg
      181 ccgccagagc aaggcgcact ggcggcagcc gggctcctga aagatcgggc gaataatccg
      241 ctgccagcat ggagtgcagg gtgactgaaa gccaggggac gacggtaccc cacgggatca
      301 gggagagcag caaacctgtt acgatgagca atccgcccag agccagaggc agccagccgg
      361 gcagaggccg gcgagcttcc ggtgcgctgg cccataatga cggagtacct tgtccggctg
      421 aatgtgggcg agcctgtggc ggctccgccg cgggcacacc cgaaggctcg ctttcagtcg
      481 gcatgtccgg catcctgtac ccgtggagcg ggaggcggtt gaacagccgg cgatccggcg
      541 ccgaggtcag gtgctgctgc ctggctggca gcgtccgcag ctcgctggct gtccgggcgg
      601 agtctggcag cgctgtcacc gggacgcatc cggtaaaccc acaagacctc ggatttgcct
      661 ttcagggcat gctcacccag cgggtcgaag cggaaatcga gccgctgctc gcccagagca
      721 gagagcgtat tttcaccgac cacgcacccc gtctcgccaa actggcgagt cagctcctgg
      781 atgcgctggg cggtgttgac cgtatcaccg atgatcgtgt aatccaggcg gtcggtagcc
      841 cccagcccgc cgaccattaa catgccggtg ttaattccaa aaccagtgat catcggcgga
      901 tcgccgcgca gggagcgctc ggcattatac tcgttgacca cctgcatgag tgcgactgcc
      961 gcctggcagg cctggaagct gctctgctcg gaaggcaggc gggtaggcag gatgccaaag
     1021 aaagccatca tcgaatcgcc ctcaaactta tcgatcaccc cgccgaactg gttgacgacc
     1081 ggtaccattc gcccgaagta ctcattcagc catttcaaga tggtgggagc gtcttctttt
     1141 tcagccaggt tggtgaaatt gcgaatatcg ctgatcagga ctgtccccac ggtgctttgc
     1201 ccttccagac gaagctcgcc ggaagcaaaa gattcgcgca gggcatcccg aacctgaggc
     1261 gagacggtgc gtcccaggag atcgcggtag atcgagcctt cctgaagccc ggcaaccatg
     1321 taattgaagg ttgtcgccag gacgctgatc tcgtcatttc cagacggttt caccttaatc
     1381 tgaaaattcc cttcagctac ccggcgggaa gcgtccacta cctgcttcag aggcagggta
     1441 atcatgcgcg cagtcaacag acctaccaga ataacagcca ggaagatcag gccgataaag
     1501 accatcacct gcacgccggt cgcctggcgc ggggcggcca gcaggttggg cgccagggcg
     1561 attcccatta tgcccaggtc ggcgccgctg cgcgcttccc agggcccgag gatttcggtg
     1621 taggtcgatg aagcactctg gatgtcgcgc tgaaagctgc tctggtcctg gttgagcagc
     1681 acgcccaggc tgtcggcagg gtcgagcggc tgtacccggg tgggttcaaa caaggtcgaa
     1741 gccagcggcg cgccatccgg cgtgtaaaat gtgatgtggc ccagggtgga ttggcgcagc
     1801 tggcgcgcaa gggagggcag ggttttccca accagcacca ccccgaccgg ctggttgttc
     1861 agatcgcgca caggaccggc aatataaaat atctgctcgc tatccccagg tgccaggccg
     1921 gcgaatttat cgcccaggat atccacatcg cctaaaagga cccgctgcac aaagggctgc
     1981 tcggccagaa gccggctgtt gcgcgtggcc cgatattctc ccggaggatc ctccggattg
     2041 cgatgcaagc cgatcaggct ggttccggtc ctatccagga ccgcgaccgc ttcttccccg
     2101 gcattgatcg ccagcggcag gaccaggctg cgcaggcgct cggcgtcgcc cgcctcgatc
     2161 gcctcggaaa ccccttcggt gttggcaatc aagcggagtg tctccagccg gcgctgctct
     2221 tctgccacca tccaatctgc tgccagacgg ccagtatcgg cgagctggtt ggtaaagcgc
     2281 tcctggagtg attcctggat ataacgcccg atcagaaaag ccgccattaa cgtgaaggtc
     2341 agcgccagga gtacatacgg cacagtaatc ttaaaccgca tcgggatgcc cacgcgcggg
     2401 atggcgcgtt cgcccggcac aggggtgacg ggcggaagtc tcgcaggcga ggcctgccgg
     2461 agagcgcgct cgatccagat gggggtaaag ggctcacgca gaaagatggc tgcgccgagc
     2521 tcgcgggcct gtatttcggc gcgcagatca acctggcggt tggtgatcac caactgggcc
     2581 atgggggctc gccgccggca ctggcggatc agcgccaggc ctgcgccatc cggggcatcg
     2641 agatcgatca gcgccagctc aggaggttca gaatcaagca gcgccacggc ttccgacagg
     2701 cgggcagttt tccagacccg gtcaccctgg ttaaggaaat agcggctcag atttttagcc
     2761 tgaagcgggt cactttgaaa gaccagtacc cgtctgggca tggtgcagcc tcccaacgcc
     2821 tggggacagg ggtcgttgaa ccctcctggt caggcggata tcttctattc aagaatacca
     2881 gaagaggtgc ctgcgcgcgg ctgcgatccc cggtcggcgc ggattacaac tttgtcagga
     2941 atccccggcg gcaagcagtt ttgtatcctg aattataatc tgtcgcatta gattgcatgg
     3001 aatatgccag ccaggcctga ataagaaaca gcgcgctttg agcgcgctgt ccaaatgccg
     3061 gtcgaaggtg aatcaatgac ttgaggcgcg ggtgcgtttc tcaatgcccg atactaccct
     3121 ggctgagaac agggtcatca ccagatagat cagggcgacc agggaccaga cctcgatagg
     3181 gttgaaattg acagacatga attcacgccc ccggcgggtc agatcggcaa cggcgacaac
     3241 gctgaccaga gatgaatcct tcagcatcgc gacaaattca tttcccacgg gcggcaagac
     3301 cacgcgcacc gcctggggta agatgacaaa gcgcatggac tgaaaatacg tcatacccag
     3361 gctgcgggca gcttccatct gtccattcgg gatactctgg atcccggcgc ggtaaatctc
     3421 gctcatgtaa gccccgtagc agatcgtcag accaatgatc gccatcgcgt tggcattggc
     3481 tgaataatta gccagggcgg ggatgccgcg ggcctgccca aaacttttca ggatcgccgg
     3541 aaacgcgaaa taccaccaga tcagctgcac catcaatggg atgccgcgga tcaactcgac
     3601 atacagggtt gagacagcaa agacaagccg gtttttagag aggcggccca gaccgccgat
     3661 taatccggcg atcaaggtaa acaaaaatga cagcgcggtg atatagatcg taacccacag
     3721 gccgtcgcgc acaaacagaa gaatccgtcg atatggatcc ggctggccga ccaccatgac
     3781 gataattaac accacaacca gcaacacgag gccccaccac gcatcaatgc gggcgctggg
     3841 ttcagcgccg gggacgtatg ccagggcttt ttcatccgcg gtaagggcgg aaggggtaga
     3901 tttggccatc ctgcctcctt cgtcttttat tgatcaaaat agggtgtctc cccgctgaaa
     3961 aagcaggcag acaccccagg ctcgtcaggt gatcgggtag agattgtagt ctcgtaccgg
     4021 gtaaagttca gagagtgtgg gcttcgatgg tgatacgtcc catataaaga tgagccgcct
     4081 tcggcagaag tgctagcctt cggcagaagt gctagccttc gcctaccagc cacttctggg
     4141 tgagcgtctc gagcagtcct tcagctctaa ccgcctcaag gccggtattg attttagcga
     4201 gcagatccgc gttggtcttg cagacggcga tgccatagga ttcatcggtg aaggggtcgc
     4261 caacgatctt cagctcggct gaattcttgg agacataacc tcgcgccagg ccatcatcgg
     4321 ccacaacggc atccacctgc ccattgatca ggtcctggta ggccaggcca atatcgtcat
     4381 aggtcttgag ggttgcaccg gcaatttctt caataacaat tgcaccggtt gtcccgatct
     4441 gtgctccgac ggtcttcccg gacaggctgt ctttgccggc gatatcggag ttatctgctc
     4501 gaacagccac cacctgcccg gcgctaaaat acgggccgga gaaggcaaaa tcggccttac
     4561 gttcttcggt gatggtaatt gccgaaatcg cggcgtcata ctggcaggag gccatacccg
     4621 ccaggagtgg gtcaaagcca acattgacaa actcgacttc gaaaccgcct ttttcggcga
     4681 tcgctttcat caggtcgata tcgaagccaa taatctcttt ggtggtttca tccacaattt
     4741 caaatggcgg ccaggtagca tccgtggcga cggtgacctt ttctccccca ccggaagagc
     4801 aggcagccag aatgaggctc aacagcatta ccagagccat cagaatccag gatcgacttt
     4861 tcatcttctt cctcctatcg aattgcaagt gaattaatat ttgggcgtaa tgaaagcaaa
     4921 gtgaatagat tcatccgtga atagtcatca cctgcctttc agactggctc gttcaacagc
     4981 tcggttgata aaaaagttta ctacaacggc ctatatgtgt ccagtaaccg gcgagtacct
     5041 acattcctgg acactcccgg ttacgcagta acccggcagg gtgaacgatg attatcaaaa
     5101 aaccgccctc aggcggttcc ttgtgtgcgc tgggagggac ttgaaccctc acgccttgcg
     5161 gcacatggcc ctcaaccatg cctgtctgcc agttccagca ccagcgcaaa gtagtggtat
     5221 tataatcacc atcacaattc tgtcaagcaa cgctgaatct aagcttcaga ttgaaatttc
     5281 attctcatct gcagcccatt cacgccactg aaagggtgaa agggctgctc accggaggat
     5341 ttatggtccg tatcgcctta gatgcaatgg gcagcgacga ctgcccggaa cctgagatac
     5401 aagccgcagt tcaggccggg cggctgtatc cggatgagat tttcctggtc ggcccgcaag
     5461 accgcctgga ggcacgcctg caagcccttg gcggcaagca tccccgggtg cgtatcattc
     5521 atgcgccaga aacgatcacg atggaagaca agggtctgaa a
//
LOCUS       DATMXK010000211         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_22978, whole genome shotgun sequence.
ACCESSION   DATMXK010000211 DATMXK010000000
VERSION     DATMXK010000211.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22978"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1166
                     /locus_tag="VN363_03925"
     CDS             <1..1166
                     /locus_tag="VN363_03925"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0070726 - cell wall assembly [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWQ83687.1"
                     /translation="PWDGAKRVTVLTMGLDYRDWQAGEGPPRTDSMMLLTIDPVNGTA
                     GMLSIPRDLWVNIPGGFGYGRINTAYQLGEGNRLPGGGSQLAMDTVEELLGVPVDYYA
                     QIDFNAFVDFIDEIGGIKLDIPEKIVVDPLGDNNSKTIKPGRQVLPGDLALAYARARK
                     TEGGDFDRAQRQQQVVLGIRDRILSLNMLPSLIAKSGVLYEQLSAGIHTNLNLDEVIK
                     LAWLAMQIPEDKITKGVISPPEQVSLYKTLEGDEVLKPITEKIRLLRDTIFTESGPTS
                     PLAVSMPAADLLKAENARVGVLNGTYEAGLAAETSEYLKTQGLNIVEAGNAQQVTTYT
                     EVTFYTGKPYTVKYLVELMKIDAIRIYHINDPASPVDVRITLGTTWANDNPMP"
     gene            1223..>2977
                     /locus_tag="VN363_03930"
     CDS             1223..>2977
                     /locus_tag="VN363_03930"
                     /inference="COORDINATES: protein motif:HMM:NF024991.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ83688.1"
                     /translation="MENLNGSIERITYYNSENGYSVLRLRPEESRPAGADRQGLVTIV
                     GNLPELQPGEHVALQGHWLNHPKHGSQFQVEICEQTMPATVAGIRRYLGSGLIKGIGS
                     RLADRIVAQFGDQTLDVIETDPERLLEVPDIGQKRARLIAAAWQDQQQVKQLMLFLHG
                     HGVSTNLAVKIYKQYGEQSLVVVQTDPYRLSQDIFGVGFKTADHLAQALGLAADHPGR
                     IEAGLEYALSEMSSNGHVYAPQGQLIERAAALLELEASKIMPAIDSLASQGRIQREVI
                     PEGTDTRPQDPLGIRENHQEYGLPAIYLMPFYRGELGVAERLRALAAHWPTHLSDLPA
                     AFLHLDTSLSAEQEEAVQRALRSPVSVLTGGPGTGKTTAVRALIEALETGHKTYALAS
                     PTGRAAKRLSQATGRPASTIHRLLGYSPGEGFKHQTGNPLPVDFVVVDETSMLDLLLA
                     NHLLKAIEPGTHLLLVGDVDQLPSVGAGDVLRDVIGSDIAPVTRLTTIFRQAAGSEII
                     TNAHRINQGQFPLFSKESTDFFLFPAETPEEAGNWVQELVCERIPKKFGLRWEDEIQI
                     LSPMYRGAVGVTTLNERLQ"
BASE COUNT          711 a          744 c          828 g          694 t
ORIGIN      
        1 agccctggga tggcgctaaa cgcgtgactg tgctgacgat gggcctggat tatcgcgact
       61 ggcaggctgg agaaggtccg ccacggactg attctatgat gcttttgacc atcgacccgg
      121 taaatggtac tgcagggatg ctttccattc cacgcgactt atgggtcaat atccccggtg
      181 gttttggtta tgggcggatt aatactgctt accagctggg cgaaggtaac cgcttacctg
      241 gcggtggctc acaattagcg atggacactg tggaagagtt gttgggggta ccggtcgatt
      301 actacgctca gattgatttc aacgcctttg tggattttat tgatgaaatt ggcggtataa
      361 aactcgatat tccggagaag atcgttgtag accctctggg tgataataac tccaagacca
      421 ttaagcccgg tcgccaggtc ttaccgggtg atcttgcctt ggcctatgcc agagcgcgca
      481 agaccgaggg cggtgatttt gatcgcgccc aacgccagca gcaggttgtc ctgggtattc
      541 gcgatcgtat cctcagtttg aatatgcttc ccagcctgat agcaaagtcg ggagtgctgt
      601 atgagcagct ctcagcaggt atccacacca atctcaatct ggatgaagtg attaaattag
      661 cctggctggc aatgcaaata cccgaggaca agataaccaa aggcgtcatc agcccgccag
      721 agcaagtcag cctttacaaa acgctcgaag gagatgaggt tctcaagcca atcactgaaa
      781 agattcgctt gttgcgcgat actatcttta cagaaagtgg accaacaagt cctttggcgg
      841 ttagtatgcc agctgctgat ttattaaaag ctgaaaacgc ccgcgtgggt gtgctcaatg
      901 gcacctatga agcgggcctg gctgctgaaa ccagtgagta cctaaaaacc cagggcctga
      961 atattgtgga agctggcaac gcccagcaag tgactactta taccgaggtt acattctata
     1021 cgggtaaacc ttatacggtt aaatatctgg ttgagttgat gaagatcgat gcgattcgta
     1081 tttatcatat taatgaccct gccagcccgg ttgatgttcg catcacatta ggaactacct
     1141 gggccaacga taatccaatg ccctgattag tgcttgcgcg cctggaggac tcagaaggtc
     1201 gtccgctcag gccggaaaag ccatggaaaa cctgaacggt tcgatagagc gcatcaccta
     1261 ttacaattca gagaatggtt acagtgtgct gcgactacgg ccggaagaga gccgcccagc
     1321 aggagctgac cgccagggtc tggtgacgat cgttgggaat ctacccgagc tgcagccagg
     1381 tgagcacgta gccctccagg gacactggtt aaatcacccc aaacacggct cacaatttca
     1441 agttgagatc tgtgaacaaa ccatgcctgc taccgtagcg ggcatacgcc gttatttggg
     1501 atcaggcttg atcaaaggga ttggttcgcg actggcagat cgaattgttg ctcagtttgg
     1561 cgaccagaca ttagatgtta tcgaaactga ccctgaacgc ctgttggagg tgcctgatat
     1621 tgggcaaaaa cgcgcccggt tgatcgcagc tgcctggcaa gatcagcaac aggtaaagca
     1681 gctcatgcta tttctgcatg gtcatggggt aagcaccaac ctggcagtca agatttataa
     1741 acagtatggt gagcaatccc tggtcgttgt gcaaactgac ccttatcgct taagccagga
     1801 tatttttggg gttggtttta aaacggctga ccatctggcc caggcattgg gattagctgc
     1861 ggaccatccc ggacggatcg aagccgggct ggaatatgcc cttagcgaga tgtccagtaa
     1921 tgggcatgtc tatgcacctc agggacaact gatcgagcgt gctgccgcgc ttctggaatt
     1981 agaagcaagc aaaatcatgc cggctataga tagcctggcg agccaggggc gaattcagcg
     2041 agaggttatt ccggaaggga ccgatacccg tccacaggat ccccttggga taagggaaaa
     2101 tcaccaggag tatggactgc cggcgatcta cctgatgccc ttctatcgcg gggaattggg
     2161 tgtggctgaa cgtctgcgcg cattagctgc ccactggccc actcacttga gcgacctgcc
     2221 agctgccttc ctccacctgg ataccagcct ttctgctgag caagaagaag cagttcaacg
     2281 tgctttgcgc tctccggtaa gtgtcttgac gggtgggccg gggactggca aaacaacagc
     2341 tgtgcgggca ttaatcgagg ctttggaaac cggccataag acctatgccc tggcgtcacc
     2401 tacaggtcga gcggccaagc gcctctcgca agcaaccgga cgccctgcca gcaccattca
     2461 ccgcttgctc ggatattcac cgggagaagg ttttaaacat caaacaggta atcccctgcc
     2521 ggtagatttc gtggtggtgg atgaaacctc gatgctggat ttactactcg ctaaccatct
     2581 cctgaaagcg atcgagccgg gtacccatct ccttttagta ggtgatgtgg accagctgcc
     2641 ttccgttgga gcgggtgatg tgctgcggga tgtcatcggc agtgacatcg cgccggtaac
     2701 gcggttaacc acgatcttcc gtcaggcagc cgggtccgag atcatcacca atgctcatcg
     2761 catcaaccag ggccagtttc cgcttttttc gaaggaatcg acagactttt tcctctttcc
     2821 ggcagagact cctgaggagg ctggtaattg ggtgcaggag ctggtgtgtg agcgcatccc
     2881 gaaaaagttt ggcttgcgtt gggaggatga gattcagatc ttatccccca tgtaccgggg
     2941 agctgttggg gttactacgc ttaacgagcg cttgcaa
//
LOCUS       DATMXK010000212         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_34727, whole genome shotgun sequence.
ACCESSION   DATMXK010000212 DATMXK010000000
VERSION     DATMXK010000212.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34727"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..279)
                     /locus_tag="VN363_03935"
     CDS             complement(<1..279)
                     /locus_tag="VN363_03935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83689.1"
                     /translation="MDTDEKITIIEGPPPTFEAINDGWALGLNEGPYLAELALTRLRT
                     FNGPSLVERCYRAWHNKQNIQLEYRSSDGLEETAPIVAARSLETVDGQV"
     gene            432..782
                     /locus_tag="VN363_03940"
     CDS             432..782
                     /locus_tag="VN363_03940"
                     /inference="COORDINATES: protein motif:HMM:TIGR02118.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EthD family reductase"
                     /protein_id="HWQ83690.1"
                     /translation="MHKLVILFEQPEDSQAFDDVWPKFLHLVEEMPGLRREAISRVES
                     FMFGSSQIAQMHELYFDTFEEAQQAMASPQGQAAGQMLQEMTGGQMTLFIADHKEDNL
                     SRIQRYRTKPVKHT"
     gene            859..1584
                     /locus_tag="VN363_03945"
     CDS             859..1584
                     /locus_tag="VN363_03945"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWQ83691.1"
                     /translation="MTPRTRRNFLLLIALIVAISLIGFVGIQILRSRSTSVSRTGRLL
                     SFLGNPQDHTDWMIPAGSRCGTAPFIFPTDGYIGFIWDDSFRPGHRHQGIDIFGGQDP
                     NTTPVVAAYPGYLSRLDDWKSTVIIRIPSDPLQPDRQIWTYYTHMADRRGNSFINADF
                     PPGTHDLFVEAGTLLGYQGNYSGTPGNPTGIHLHFSVVMDDGQGKFLNELEIANTYDP
                     SPYLGLELNANSGITNIPLCATN"
     gene            1628..>2034
                     /locus_tag="VN363_03950"
     CDS             1628..>2034
                     /locus_tag="VN363_03950"
                     /inference="COORDINATES: protein motif:HMM:NF012334.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ83692.1"
                     /translation="MGSFQNQIVLVTGAGRSRGRSIAQAFASQGAIIAANDLTPINLD
                     ETVRQITANGGQAKEYIFDIAKKMPVQALLAQVLEDWGQLDICIHCAQVKPKAALLDM
                     DEWDWRRTIDVNLSSVFFLTQSAGRIMRQQGHGR"
BASE COUNT          492 a          538 c          516 g          488 t
ORIGIN      
        1 aacctgtcca tctactgtct caaggctgcg agcagctaca atgggcgcag tctcctccag
       61 gccatccgac gagcggtatt caagctggat attctgttta ttgtgccagg ctcgatagca
      121 acgctcaacc agggatggac cattaaaggt gcgcaagcgg gtgagggcca gctcagccag
      181 gtaggggcct tcattcaagc ctaacgccca cccatcgttg atcgcttcaa aagttggggg
      241 gggaccttcg ataatggtaa ttttttcatc cgtatccatt ggctaattct ccataatggg
      301 ggcaatcata ccataaagga agttcttcac cagcaaaaac ctttaaatac agatgagcat
      361 catttaatcg aatatctcga ctttcgatac tcggatgtgg cgcgagtata atgaaacttt
      421 ggagctgccc tatgcataaa ctcgttatct tatttgaaca acccgaggac tcacaggctt
      481 ttgatgatgt ctggccaaaa ttcttacacc tggtggaaga gatgcccgga ttacgccgtg
      541 aagcgatcag cagggtggag agtttcatgt ttggctcttc gcagatcgcc cagatgcacg
      601 agctgtattt tgatacgttc gaggaagctc agcaggcaat ggcatcccca caagggcagg
      661 ctgccgggca aatgctgcaa gaaatgaccg ggggccagat gactttgttc attgccgatc
      721 ataaggaaga taatctgagt cgcattcagc ggtatagaac caaacctgtt aagcatacat
      781 aacccttgat cctctgatga gccggacaaa actgtctcaa aatattgggc gatcactcca
      841 actccatccg gatccggcat gactcctcgc acccggcgta attttcttct cctgatcgct
      901 ttaatcgttg cgataagcct gattggcttc gtggggatac aaatcctgcg cagcagatct
      961 accagcgtga gccgtacggg ccgcctgctg agctttctgg gcaacccgca ggaccatacc
     1021 gactggatga tccctgccgg ttcgcgctgt ggcactgccc cgttcatttt ccccaccgat
     1081 ggctatatcg gatttatctg ggacgactcg ttccgacctg gacaccggca ccaggggatc
     1141 gatatttttg gtgggcagga tcccaataca acgccggtgg tggcagccta cccgggatat
     1201 ttatcccgac tggacgattg gaaatcaacc gtcatcatcc gcatccccag tgacccactc
     1261 cagccagatc gccagatctg gacctattac actcacatgg cagataggcg cggaaattcg
     1321 tttatcaatg ctgattttcc cccaggtaca cacgatttat ttgtagaagc tggcacgttg
     1381 ctcggttatc agggaaatta ttccggtaca ccaggtaatc caaccggtat ccatctccat
     1441 ttctcagttg tcatggacga tggtcagggc aagttcctga atgaactgga gatcgccaat
     1501 acttacgatc catcgccata tcttggcctg gagctcaatg ccaattcggg cataacgaat
     1561 ataccccttt gtgcaaccaa ttaatcagct gctggcgctg ccacagtcac tgcaggaggg
     1621 tcgctcgatg ggttcatttc agaatcagat agttttagta accggagcgg ggcgcagccg
     1681 cgggcgttcc attgcgcaag catttgccag tcagggcgcc attatcgccg ccaacgacct
     1741 gaccccgatc aatctggacg agactgttcg ccagatcacg gcgaatggtg gtcaggcaaa
     1801 agaatacatc tttgatattg ccaaaaagat gcccgtccag gctttgctcg cgcaggtgct
     1861 ggaggattgg gggcaattgg atatctgcat ccactgcgcc caggtcaagc caaaagctgc
     1921 cctgttggat atggatgagt gggattggcg gcgcacaatc gatgtgaatc tttcatcagt
     1981 tttcttcctg acacaatccg ccggacggat catgcgccag caaggccatg ggcg
//
LOCUS       DATMXK010000213         3619 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_36208, whole genome shotgun sequence.
ACCESSION   DATMXK010000213 DATMXK010000000
VERSION     DATMXK010000213.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3619)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3619)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3619
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36208"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..473)
                     /locus_tag="VN363_03955"
     CDS             complement(93..473)
                     /locus_tag="VN363_03955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83693.1"
                     /translation="MKKLTTIALAIVVLAAGFFYSSARAETISDCQALIDKTSSDLAG
                     VTIGGNNPAQTRASLLSKLAGASTKLDEGKYQDAIGKLVDFRTSVEEMLSAPKPKISP
                     EDAALLITDANNAIACIQSLIVEG"
     gene            complement(649..915)
                     /locus_tag="VN363_03960"
     CDS             complement(649..915)
                     /locus_tag="VN363_03960"
                     /inference="COORDINATES: protein motif:HMM:NF024835.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix transcriptional regulator"
                     /protein_id="HWQ83694.1"
                     /translation="MQERYSDTQTFCGNLYGRLRLQRGLTQAQLAELVGTQQPSIARL
                     ENGSSIPSLSFLHKIASALNARIEVRLVAEPQEKYRVNPPNTDA"
     gene            complement(1230..1601)
                     /locus_tag="VN363_03965"
     CDS             complement(1230..1601)
                     /locus_tag="VN363_03965"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83695.1"
                     /translation="MMIGMRPLINLDLAFDEALAWLTGRLLPARLRVVRTFDLQETFT
                     DPADCPCQHPPGEVCSCRMVVLLVYGEGYLPASLVALGNLDRTCFSLVDSPQQRLEAG
                     LEIGIRQALRYEDVLVAEPVD"
     gene            complement(1678..1833)
                     /locus_tag="VN363_03970"
     CDS             complement(1678..1833)
                     /locus_tag="VN363_03970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83696.1"
                     /translation="MALAPGETTTLSMQFTMHSGMEGKHNFRVHLPSNDPAQPDRTLT
                     VLSNWVP"
     gene            complement(1858..2169)
                     /locus_tag="VN363_03975"
     CDS             complement(1858..2169)
                     /locus_tag="VN363_03975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83697.1"
                     /translation="MTQPKLKKYQKPRRRPFRWQPLLLAVGGLALVVAAFFALQNKPD
                     PGAEIEVSGAPSLKVDQELIDLGDIKVDQPAQATFTLSNTGDQPLKLAQEPYIEVVEG
                     C"
     gene            complement(2172..3239)
                     /locus_tag="VN363_03980"
     CDS             complement(2172..3239)
                     /locus_tag="VN363_03980"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWQ83698.1"
                     /translation="MRKEWYARILVITLAAAAVAVPLLVRRVSSQPPLLHARMAETGG
                     WSLAGSQAENPGDLTAVAGEPLRLRLTSDDVVHSFAVGQAAGMPEGIAAVDILPGEIS
                     EITLNFDRPGKYTYYCTRWCSVNHWRMRGVIEVSPPAAGVASGSDTPASEAPDPELPL
                     YLQLGIDPDAERPDPAVLPARPPSAARGAALEADIPAEFLGQDYYRAHSPAELWSSLR
                     ATSSLAGLTDQDVWDLVALAYQAQTTPAALQSAAETYSQNCAACHGERGGGDGVFADD
                     LALPETGEHASMPDGEMTTTPAGFSDPARLLSASPALLHGKILRGGMGTGMPYWGPIF
                     TADQIWDLVAYLYTFQFAQEP"
     gene            complement(3243..>3619)
                     /locus_tag="VN363_03985"
     CDS             complement(3243..>3619)
                     /locus_tag="VN363_03985"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83699.1"
                     /translation="SAAQLRRAIVRQSQALLPLGLFAWIAFTLSFALPKLTTVLAVLS
                     DPFGWGWNLFGTAQLTWAPDFSAASSLLQVALLLVGLFWSARLAVQNSSAGDPPGRPY
                     KAVPVAFFSLAYTLVLFALLVG"
BASE COUNT          762 a         1017 c         1105 g          735 t
ORIGIN      
        1 ggttttcaac cggagtactg gcctgaaaac ccgcacaagt gcgctgaact tcacactcga
       61 gttcataaag acagaagttt tttgctcctg ctctaccctt ccacgatcag gctttggatg
      121 caagctatgg cattgttggc gtctgtgatc aacagtgctg catcttcagg gctgatcttt
      181 ggttttggag cagataacat ctcctccacc gacgtacgga aatcgaccag tttaccaatg
      241 gcatcctggt atttaccttc atccagcttg gtggatgcac cagccagctt cgacaggaga
      301 ctggctcgcg tttgtgctgg attatttcca ccaatggtca ctcccgccag atcactggag
      361 gtcttatcga taagtgcctg acaatccgat atcgtttctg ccctggcaga agaatagaaa
      421 aagcctgcag ccagaacgac aatcgcgagc gcaatagtgg tcagtttttt catagtagat
      481 cctttcacct attcttgact ttccggatgt aaatctgtga tggcgaatag ttatccgcct
      541 ggagagatat acggataaac aaattagttg gatttatcgg agcgcattga atctgggtat
      601 aaataaccta gacccctggg gttttcgaaa gccccagggg tcttttgatc atgcatctgt
      661 attcggtgga ttaacccggt acttttcctg tggctctgcc accagcctga cttcaatccg
      721 ggcgttcaac gctgacgcta tcttgtgtaa aaatgacagg ctggggatac tcgagccgtt
      781 ttccaggcgg gcaatggaag gctgctgggt accgaccagc tccgccagct gcgcctgagt
      841 caggccgcgc tggaggcgca ggcgaccata caggtttccg caaaacgtct gcgtatcgga
      901 ataacgctct tgcaccaggc ttcttcgaat ctgactgcct taaaacctgt acggtctgtc
      961 catggatgga tgaatagacc atacaggttt taatgatttc tgagtatgca gggtaattac
     1021 gcgttcatgt tagctgtaac ggcaaggttc tacggaaacc tgtatggtcc gttatcgcgc
     1081 tcgcgatttc ggcagcaatc gatccgaatt tcatataacc tgtacggtct gtccatgatt
     1141 gttcgaaata accaaccgca gatgacgcag atcggcgcag ataagaccag ataatatctg
     1201 cgttcatctg cgatatctgc ggtaatcgct taatccactg gttctgcaac caggacatcc
     1261 tcgtatcgca gcgcctgccg gattccgatc tccaatcccg cctccaggcg ctgctgcggg
     1321 ctatcaacca gcgagaagca ggtccgatcg aggttaccca gggcgaccag gctggcgggc
     1381 aagtagcctt ccccatacac cagcaggacc accatccggc agctgcaaac ctcgcccggc
     1441 gggtgctggc agggacaatc cgccgggtcg gtaaaggtct cttgcaggtc gaaggtgcgc
     1501 accactcgca ggcgcgccgg caggagccgg ccggtgagcc aggcaagggc ttcgtcgaaa
     1561 gccaggtcca ggttgatcag ggggcgcata ccaatcatca ttcacttcct aaatttcgtg
     1621 agtacgggat aataagtctg gatgttttta tcccgatctg atgataaatt agttctttca
     1681 ggggacccag ttggaaagca cagtcagggt gcggtcgggt tgggcagggt cattgctggg
     1741 caggtggaca cgaaaattat gcttgccttc catgccggaa tgcatggtga actgcatgct
     1801 gagggtggtc gtctcgccgg gagccaatgc catcgaaccg atggcgggaa caggcggtca
     1861 acagccctcg acgacttcga tatacggctc ctgggccagt ttcagcggct ggtcgccagt
     1921 gttgctgagg gtgaaggtgg cctgggcagg ctggtcgacc ttgatgtcgc ccaggtcgat
     1981 aagctcctgg tcgactttca ggctgggcgc gccgctgact tcgatctctg cacctggatc
     2041 aggcttattc tgtaaggcga aaaaagccgc caccaccagc gccaggccgc ccacggccag
     2101 cagcagcggc tgccagcgaa aaggccggcg gcggggtttt tgatatttct tgagtttcgg
     2161 ttgggtcata ttcagggctc ctgtgcaaat tggaatgtat acaggtatgc gaccagatcc
     2221 cagatctggt ccgcagtgaa aattggtccc caatagggca taccggtgcc catgccgccg
     2281 cgcaagatct tgccgtggag cagggcaggg ctggccgaga gcagccgggc gggatcgctg
     2341 aaaccggccg gggtggtggt catctcacca tccggcatac tggcatgctc gccggtttcc
     2401 ggcaaggcga ggtcatcggc gaagacgcca tccccgccgc cccgctcgcc gtggcaggcg
     2461 gcgcagttct gcgaatacgt ctcggccgcg gattggagcg cggccggggt tgtctgcgcc
     2521 tggtaggcca gggcgaccag atcccagacg tcctgatccg tgagccctgc caggctggaa
     2581 gtggcgcgca ggctgctcca cagttcggcg gggctgtgag cgcggtaata atcctgcccc
     2641 aggaactcgg ccgggatatc agcctccagg gcggcgccgc gcgcagccga gggtggccgg
     2701 gccggcaaga cagccggatc tgggcgttcg gcgtccggat cgattcccag ctgcaggtag
     2761 agcggcagct ctgggtcagg cgcttcgctg gccggggtat ccgatccgga tgcaacaccg
     2821 gctgcaggcg gggatacttc gatcacaccc cgcatccgcc agtggttgac gctgcaccag
     2881 cgggtgcagt aataggtata cttgccgggc cggtcaaagt tcagggtgat ttcgctaatc
     2941 tcgccgggca ggatatccac cgccgcgatg ccctcaggca tcccggcggc ctggccaacg
     3001 gcaaagctgt ggaccacatc gtccgaggtc aggcgcaggc ggagcggttc gccggctaca
     3061 gccgtgagat cgccaggatt ctcggcctgc gatcctgcca ggctccagcc gccggtctcg
     3121 gccatgcggg catgaagcag gggcggctgc gaacttaccc ggcgcacgag cagcgggaca
     3181 gccacagcag ctgcagccag ggtgatcacc agaatacgag cataccattc tttgcgcatc
     3241 gtctatccca ccagcagcgc gaacagaacc agcgtgtaag ccaggctgaa gaaggcgacc
     3301 ggaacggctt tgtaggggcg acccggcggg tcgcccgcac ttgaattctg gacagccagc
     3361 ctggccgacc agaacagacc cacgagcagc agggcgacct ggagcaggct gctggcggca
     3421 gaaaaatctg gtgcccaggt caattgcgcc gtgccgaata aattccagcc ccagccgaag
     3481 ggatcggaga gcactgccag gacggtggtg agcttgggca gggcgaagga aagggtgaag
     3541 gcgatccagg cgaacaggcc gagcggcagg agcgcctgag attggcgaac gatggcacgg
     3601 cgcagctgcg cagccgagc
//
LOCUS       DATMXK010000214         3080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_4336, whole genome shotgun sequence.
ACCESSION   DATMXK010000214 DATMXK010000000
VERSION     DATMXK010000214.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4336"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1172
                     /locus_tag="VN363_03990"
     CDS             <1..1172
                     /locus_tag="VN363_03990"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ribonuclease J"
                     /protein_id="HWQ83700.1"
                     /translation="TGDYKFDHTPIDNWPTDYAKLAELAGRGVLALMADSTNADKPGW
                     TPSERVIDPAFDKVFREAKGRIIIASFASLISRMQQVGNAAQRHGRKMAFVGASMVEN
                     AKIARKLGYLDLPDDLVVPLDQALKLPQNQVVLMSTGTQGEPTSIMGRLSTGTNRQFD
                     ITPSDTVVLSSHPIPGNEESVYRTINRLFRRGARVIYEGIAPVHVSGHARQEEMKLLL
                     HLVRPKYFIPIHGELRHLHQHAQIAQEVGIPAENIAVIENGQVVEFQNGEMHLGERIK
                     GGWVFVDGYGVGDVGPSVIREREALARDGFVLVNLILDRFSHRLLEDPEIITRGFIYT
                     RNGGEDLLDETRALVKETISSGTANLENDVQETVKTFLFNKTRRRPMIFVSMTRA"
     gene            complement(1215..1916)
                     /locus_tag="VN363_03995"
     CDS             complement(1215..1916)
                     /locus_tag="VN363_03995"
                     /EC_number="2.1.1.77"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560308.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004719 - protein-L-isoaspartate
                     (D-aspartate) O-methyltransferase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein-L-isoaspartate(D-aspartate)
                     O-methyltransferase"
                     /protein_id="HWQ83701.1"
                     /translation="MSTLQANKDDLERFYAEQRRKMVHEQLARRGIVDKRVLQAMSEI
                     PRHLFVPEEVRSAAYQDSPLPIGYRQTISQPYIVALMTQMLGLGGHEKVLEIGTGSGY
                     QAAILARLAGIVQTIERHPTLSEAARRVIHSLDISNVIFQVGDGSLGFAEQAPYDAIL
                     VTAGAPSVPEKLLEQLSERGRLVIPVGKWKAQTLERWWRTPAGLKHESVAPVAFVPLV
                     GKEGWDTSADEDAPV"
     gene            complement(1922..2926)
                     /locus_tag="VN363_04000"
     CDS             complement(1922..2926)
                     /locus_tag="VN363_04000"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR03617.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0070967 - coenzyme F420 binding [Evidence
                     IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR03617 family F420-dependent LLM class
                     oxidoreductase"
                     /protein_id="HWQ83702.1"
                     /translation="MSMILDATLPPVGLNAIAEIGRAAEEMGFGGIWSTETVHDPFLP
                     GALIAQHTRRIQFGTAVAIAFARSPAALAYTAWDLAQATHGRFILGLGTQVKAHIERR
                     FGMPWPESVTGKLGEMLGAIRAFWRAWQFNEPLNYRTSAYKLNLMTPFFNPGPIEFPE
                     VPIYIAGVNTGLARLAGESANGFLIHPFHSPEYLQQVMLPAIQEGLQRSSRTRADVKI
                     AATVFCITNPGEREFVRQQIAFYASTPSYLNVMALHGWQEIAGQLSHLAAHKQWQAMP
                     GLISNEMLATFAVESTSQELPDALTERYAGLVDRLSLYLPFVPGERDNFWRSLLKDQV
                     "
BASE COUNT          693 a          829 c          860 g          698 t
ORIGIN      
        1 ataccgggga ttataaattt gatcataccc cgatcgataa ctggccaaca gattacgcca
       61 agctggctga attggccggg cgcggtgtgt tggccttaat ggccgattca accaatgctg
      121 ataaacctgg ttggactccc tccgaacggg tgatcgatcc agcatttgat aaagtctttc
      181 gagaggccaa agggcggatt atcatcgcca gttttgcatc cttgatctcg cgtatgcagc
      241 aagtcggtaa tgccgctcag cgccacggac gtaaaatggc attcgttggg gcaagcatgg
      301 tcgaaaatgc caagatcgcc cgcaaattgg gttatctgga tctaccggat gatcttgtcg
      361 tccctctgga ccaggccctt aagctgcctc aaaatcaggt cgttttgatg tccaccggca
      421 cacagggtga gccaacttcg attatgggcc ggctatccac cggcaccaat cgccagttcg
      481 atatcacgcc cagtgatacg gttgtgcttt catcgcaccc gatcccgggc aacgaagaat
      541 cggtctaccg cacgatcaat cgtttattcc ggcgtggagc gcgggtgatc tatgaaggaa
      601 ttgccccggt ccacgtctcc gggcatgccc ggcaagaaga gatgaagctg ctcctccacc
      661 tcgtacgtcc gaaatatttc atcccgatcc atggcgagct tcgccacctg caccagcacg
      721 cccagattgc ccaggaggtc ggcattcctg ctgagaatat cgcagtcatt gaaaatggtc
      781 aggttgtaga attccagaat ggagagatgc acctgggtga gcggattaaa ggcggctggg
      841 tatttgtgga tggttacggt gtgggtgatg tcggtcccag tgtgatccgt gagcgtgaag
      901 cgctggcacg ggatgggttt gtgctggtca acctcatcct ggatcgcttt agccatcgct
      961 tgctcgaaga cccagaaatc atcacgcgtg gatttattta cacacgcaat ggaggcgagg
     1021 atttgctgga tgaaactcgc gctcttgtga aagagaccat cagcagcggt acagcgaacc
     1081 ttgagaatga tgtccaggaa actgtaaaaa cgttcctgtt caataagact cgccggcgac
     1141 cgatgatctt tgtctccatg acccgtgcct gatatcctga caatggattg atctataaaa
     1201 cgcccccggt tttgttacac gggggcgtct tcatctgcgc tggtatccca gccttcttta
     1261 ccaaccagcg gcacaaacgc aaccggtgcc acggattcgt gtttcaagcc tgctggtgtg
     1321 cgccaccagc gttctaaagt ttgggctttc catttcccaa ccggaataac cagccggccg
     1381 cgctcgctta attgttccag caatttttct ggcacgctgg gcgcgcctgc cgttaccagg
     1441 atggcatcgt agggggcctg ctcggcgaag cccaggctgc catccccaac ctgaaagatc
     1501 acattgctaa tatccaggct gtggattact ctcctggctg cctctgacag tgtggggtgc
     1561 ctctcaatgg tttgaacaat gcctgccagg cgcgccagaa tagcggcctg gtagccggag
     1621 ccggtaccaa tctccaggac cttttcgtga ccacctaagc ccagcatctg ggtcatcagg
     1681 gcgacaatgt agggttgtga gatagtctgg cgataaccga ttggaagggg actatcctgg
     1741 taagctgccg aacggacttc ctctggtacg aaaagatggc ggggaatttc agacattgcc
     1801 tgcagaactc gcttgtcgac gattccccgg cgagccagct gctcatggac catcttccgg
     1861 cgctgctcgg cgtaaaagcg ctccagatca tctttattgg cttgcaaagt agacatggac
     1921 atcagacctg gtctttaagc aaagaacgcc aaaaattatc cctttcaccc ggaacgaagg
     1981 gcagatacag gctcaaacgg tctaccagac cggcgtagcg ttcagtaagc gcgtcgggca
     2041 gctcctgtga ggtggattcg acagcaaatg tcgccagcat ttcattgctg atcaagcctg
     2101 gcattgcctg ccattgtttg tgggctgcca ggtgagagag ctgccctgca atttcttgcc
     2161 agccgtgcag ggccattaca ttcagataag agggtgttga ggcgtaaaaa gcgatttgct
     2221 ggcgtacaaa ctcgcgttcg ccgggattgg ttatacagaa aacggtagca gcaattttca
     2281 cgtcggcccg ggtgcgtgag ctgcgctgca gaccttcttg aatggctgga agcatgacct
     2341 gctgcaaata ctcaggtgaa tggaacgggt ggatgagaaa tccattggca gactcacccg
     2401 ccaggcgggc gagcccggta ttgaccccag caatatagat gggtacttcc ggaaattcaa
     2461 tcggcccggg gttgaagaag ggggtcatca aattgagttt gtatgctgat gtacggtaat
     2521 ttaagggctc gttgaactgc caggcgcgcc agaaggcgcg gatcgcgccc agcatttccc
     2581 ctaatttgcc cgtcaccgat tcgggccagg gcatgccgaa gcggcgttcg atatgcgctt
     2641 ttacctgcgt gcccaaaccc aggatgaaac gcccatgggt tgcctgggcc agatcccagg
     2701 ctgtataagc cagcgcggca gggctgcggg cgaaggcaat tgccacggcg gtgccgaatt
     2761 ggattctgcg cgtatgttga gctattaatg ctccgggcaa gaagggatcg tgaactgtct
     2821 ctgttgacca gataccgcca aaccccattt cttcagcggc tcgcccaatc tctgcgatcg
     2881 catttaatcc tacaggaggt agggtcgcat ccaggatcat gctcattgga ttgcctctaa
     2941 gataataaat acgccagcac ccattgggtg gtggcgacca gggcatccag gtgggtgcgc
     3001 tcgtaattgt gggacgcatc tacacccggg ccgatcagcg ccacagctac atccccacca
     3061 gcgcgccaca aggcttctcc
//
LOCUS       DATMXK010000215         2017 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_126273, whole genome shotgun sequence.
ACCESSION   DATMXK010000215 DATMXK010000000
VERSION     DATMXK010000215.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2017)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2017)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2017
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126273"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..712
                     /locus_tag="VN363_04005"
     CDS             <1..712
                     /locus_tag="VN363_04005"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWQ83703.1"
                     /translation="SQADLAQAFASHVAVAIENARLYEQAQSLASIQERQKLARELHD
                     SVSQALYGIALGARTARTMLDRQEKSSDFKDPLEEPLDYVLELADAGLAEMRALIFEL
                     RPESLESEGLVIALTKQAAALKARYQLNVVTEFCPEPDISLLAKETLFRISQEATHNT
                     VKHAGATKIHLSLTEQDHLLSLVIRDDGRGFNSGGDFPGHLGLRSMRERMERIGGSLN
                     ITTSPGKGTTITAQYTES"
     gene            complement(809..>2017)
                     /locus_tag="VN363_04010"
     CDS             complement(809..>2017)
                     /locus_tag="VN363_04010"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HWQ83704.1"
                     /translation="PCNRAEYVKDVTVPDGTVYTPGVAFTKTWQLKNTGSCEWSADYR
                     LIFASGKQMDGRDSVALPGKVRPGESVNLSIDLVSPSKEGKFRGYWMLRATNGEVFGI
                     GGDGEEAFWVEIRVFYPPDLGFEYDFASNYCIAAWRSGAGKLACPTTSSQEDGSINLT
                     DRPVLENGRQENEFTLIVNPEQINNGYITGTYPVYRVNTGDHFLADIGCLENNQGCDV
                     TFFLDGILSDGKVLSLGAWNESYDGRITRINLDLSSLNGQNVRFVLGLRANSRPGKAN
                     AFWLVPSIRAVQATPTPTRTNVPPTITPTPGPTQTPSPTLPSVTDSPAADAARRTVAA
                     ALGIDPDLVVVRSIEAAHWTDTCFGVATTDQNCAPASVDGFKIGMSVNNSFYEAHTNL
                     DGSIVYWFAR"
BASE COUNT          472 a          549 c          549 g          447 t
ORIGIN      
        1 gagtcaggca gaccttgccc aggcgtttgc cagccatgtc gccgtcgcaa ttgagaatgc
       61 ccgcctttac gagcaagcgc agtctctagc ttcgatccag gagcggcaga agttagcccg
      121 tgagctccac gattcggtct cccaggctct ctatggcatt gcactgggtg cccggacagc
      181 ccggacaatg ttggacaggc aagaaaaatc ttcggatttt aaagatccac ttgaggaacc
      241 tctggattat gtcctggagc tggctgatgc aggcctggcc gaaatgcggg ccttgatttt
      301 cgagctgcgc cccgaatcgc tggagtcgga aggcctggta atcgccctga cgaaacaagc
      361 cgctgcgctc aaagcgcgct atcagttaaa cgtggttacg gagttttgcc cggagccaga
      421 tatttccttg ctcgcaaaag aaacgttgtt caggatttcc caggaggcaa cccataacac
      481 tgtgaaacat gccggagcga cgaaaattca cttgagcctg acggaacagg accacttgct
      541 tagcctggtt atccgcgatg acgggcgcgg tttcaactcg ggcggtgatt ttcccgggca
      601 tctgggcctg aggtcgatgc gggagcgtat ggagcggatc gggggcagcc tcaatattac
      661 cacctcgcca gggaagggga caaccataac tgctcagtac actgaatcat gatcctggtc
      721 tttctcggac cctgaagggc aaggagctta acaaaaaggg ctgtcccggc gtacgaacgg
      781 gacagccctt atggttcagg cggtgggatt agcgtgcgaa ccagtacaca atactaccat
      841 cgaggttggt atgggcttcg tagaatgaat tgtttacact catgccgatc ttgaacccat
      901 ctacgctggc gggagcacaa ttctgatcag tggtcgccac accaaagcat gtatcagtcc
      961 aatgggcagc ttctatactg cggacaacaa ccaggtcagg gtcaattccc agggcagcgg
     1021 ctaccgttct ccgggcagca tccgcagccg gtgagtcggt cacactgggc agagtcggcg
     1081 agggtgtctg ggtcggcccc ggtgttggag tgatggtcgg ggggacattc gtccgggttg
     1141 gtgtcggggt cgcctggaca gcccggattg acgggaccaa ccagaaagca tttgccttgc
     1201 caggccggct gttggctcgt aaacccagta caaacctgac attttgacca ttcagagacg
     1261 agaggtccag attgatgcgg gtaatccgcc catcgtaaga ttcattccag gcacccaggc
     1321 tgagcacttt gccatcactt aagatgccat ccaggaagaa ggtgacatca cagccctgat
     1381 tattctccag acagccaata tctgccagga agtgatctcc agtatttacc cggtagactg
     1441 gataggtacc ggtgatatag ccattattga tctgttcagg attgacgatt aaggtgaact
     1501 cgttttcctg ccgtccgttc tccagaacag gacggtctgt cagattgata ctgccatctt
     1561 cctggctgga tgttgttggg caggcgagct taccagcgcc agagcgccag gcagcgatac
     1621 aatagttgga ggcaaaatca tattcaaagc ccagatctgg tgggtagaat acccggattt
     1681 caacccaaaa cgcctcttca ccatcgcctc cgatcccaaa cacctcccca ttggtcgccc
     1741 gcagcatcca gtagccacgg aatttacctt ctttggaagg tgagaccaga tcgattgaaa
     1801 gattcacact ttcaccaggg cggactttgc caggcaaagc tacactatcc cgaccatcca
     1861 tttgcttacc gctggcaaag atcagccggt aatctgccga ccactcacag cttccagtat
     1921 tctttagctg ccacgttttg gtaaatgcta ccccgggtgt atagacagtg ccatccggaa
     1981 cagtcacatc cttcacatac tctgcccggt tacaggg
//
LOCUS       DATMXK010000216         3132 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12596, whole genome shotgun sequence.
ACCESSION   DATMXK010000216 DATMXK010000000
VERSION     DATMXK010000216.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3132)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3132)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3132
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12596"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>3132)
                     /locus_tag="VN363_04015"
     CDS             complement(<1..>3132)
                     /locus_tag="VN363_04015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83705.1"
                     /translation="VYALGLDGAVWYREYAGTWAAWQRLESPQGVAWNAVPVLTSPAD
                     GQVNIYLAGTENRIWENLFNGSAWTGWNSTAVTGGELDAGIAAGDSPAGAFLFTQLSG
                     GSLQYSLNAAPWTPMPGLDVSHTVFDTGTITKPLPAAIPLENSYLDLATGYFTGDGRQ
                     QVVLAYADPTDQIMVEIYDVQSGFRLHKIAALSASIAAGWPRVAAGDVDGDGVDEIGL
                     ASVLDSAAAQRKLTLRVYQVAKDAGGAWTGELTEISYNEVAMDTAGGDIWHGFPFGGT
                     LRIAVGDILPEQDSNNDEFAIYSDWRHDSSGNWRGIYLYLHLYDHQSNPQISEQVSQF
                     LGATDGSYWNELYATGAGLAVGDVNGAVPGYGVDEIVITYPWEFYGHDYPDLRRRLKV
                     FGVQQNTTAAVELGSHDILDWSRHTFLDTLAVADLDQDLVDEIVMAGNFSSGNPSTGY
                     SLDYYEFASLSSGPVDRWPIPYTALPYAFNLAVGNFTGEGFQVGPPTYRQQKGMISPE
                     VLLNLPPMHRDILDGEEITIDNGAIAIYTAGNTSTLQTSSESKRDWSLSVGLETSVGA
                     AGHTVTASLDNTYGENFSKATTEISTVAFEDTTTAEDYDQVIFNATRYAIWEYPVLGV
                     ETADPDQPATIAVVLPLVDTTTSPKNEQGRKCDENFYTPGHQPYNVWSYDRLGEVLFG
                     DYDPLREVDNKETAGGRDFDIRMSTVSEAMRSDSFHNQVSAGLEYSYENSLDIPLIGK
                     AWDFSFRASTNAEYGIEEISTLTSTFTQETSVEVDFPSNPNPSNYTIKAYLYWAKAGY
                     LVLDYQTEPDPNSGDWQKYNKTDPAFILPWYGFPDPVTGLFPPSGNDQPPCGMSKQLF
                     THDIQIEPAYAENGDTVTLQATLRNFSAVIPPADVAVRFYLGTPAGGYQIGSCSIPRL
                     QLNRAAGPAGCSANWTVSGASGEERIYAVIDPQNAFDEMHDGSDVINNNVGYGLLQAA
                     NADYFDPGLRQAQVYQAILYEEAPGLNYGLYLPTSNGSDTIRYELVPTALDLLSPIVG
                     VPIQVLAYLGGEQ"
BASE COUNT          663 a          851 c          976 g          642 t
ORIGIN      
        1 aactgctcgc cgcctaagta agccaggacc tggatgggaa ccccgacaat cggagaaagg
       61 aggtccagcg cagtggggac gagctcgtag cggatggtgt cggaaccatt gctggtgggg
      121 agatacaggc cgtagttcag gccgggggct tcttcataca ggatagcctg ataaacctga
      181 gcctggcgca gcccgggatc gaagtaatcc gcattggcag cctgcagcag gccatagcca
      241 acattgttat tgatcacatc actgccatca tgcatttcgt cgaaggcatt ttgcgggtcg
      301 atgacggcat agatcctttc ttcaccggag gcgccgctga ccgtccagtt ggccgaacac
      361 ccggctggtc cggcagcgcg attcaactgc aggcggggga tgctgcagga tccgatctgg
      421 tatcccccag ccggggttcc cagatagaac cggaccgcca catctgcggg tgggatgacc
      481 gccgagaaat tgcgcagggt ggcctgcagg gtgaccgtgt cgccgttttc agcataagcc
      541 ggctcgatct ggatatcatg ggtgaagagc tgtttgctca tgccgcaagg cggctggtca
      601 ttgccgctgg gtgggaataa accggtcacc gggtccggga agccgtacca gggcaggatg
      661 aaggccgggt cggttttgtt gtacttctgc caatcgccag aattaggatc gggttcggtc
      721 tggtaatcga ggaccaggta gccggccttc gcccaataca ggtaggcttt gatggtgtaa
      781 ttgctgggat tcggattgct ggggaaatcc acttcaacac tggtctcctg ggtgaaagtg
      841 gaagtcaggg tggaaatctc ctcaatccca tattcggcgt tggtcgaagc ccggaagctg
      901 aaatcccagg ctttaccgat cagggggata tccagcgagt tttcgtagga gtattccagg
      961 ccggcgctga cctgattgtg gaagctatcg gagcgcatgg cttcggaaac cgtggacatg
     1021 cgaatatcga aatctcgtcc gccggcagtc tctttgttat ccacttcgcg cagggggtcg
     1081 taatccccga agagcacctc tccaaggcga tcataagacc agacgttata aggctggtgg
     1141 ccgggggtgt aaaaattctc atcgcatttg cgcccctgct catttttggg cgaggtggtg
     1201 gtatccacca gcggcaagac cacagcaatc gtcgccggct ggtcgggatc cgccgtttcg
     1261 acgcccagga cgggatattc ccagatggca tagcgggtgg cgttgaaaat gacctggtcg
     1321 taatcttcag cggtggttgt gtcctcgaag gcgacggtgc tgatttcagt ggtagctttc
     1381 gagaaatttt ccccgtaggt gttatccagg ctggcggtga cggtgtggcc ggcggcgccc
     1441 acgctcgttt ccaggccgac cgagagagac caatcgcgct tagattcaga agaggtttgc
     1501 aaggtggatg tgtttccggc ggtatagatg gcgatggcgc cgttatcgat ggtaatctct
     1561 tcaccgtcga ggatatcgcg atgcatagga ggcaggttca gcagcacctc cggggagatc
     1621 atgccctttt gctggcggta ggtgggcggc ccgacctgga agccttcgcc ggtgaaattg
     1681 cccacggcga ggttgaatgc gtaaggcagg gcggtgtagg ggatgggcca gcgatccacc
     1741 gggccgctgc tgagcgaggc gaactcatag taatccaggg aatagccggt ggaagggttc
     1801 ccactgctga aatttcccgc catgacaatc tcgtccacca gatcctgatc gagatctgcg
     1861 actgccaggg tgtccaggaa ggtatgtcgg gaccaatcga gaatatcatg gctgcccagc
     1921 tcaactgcag cggttgtgtt ttgctgcacc ccaaatacct tcaaacggcg cctcaaatcc
     1981 ggatagtcgt gaccgtagaa ttcccagggg taagtaatga caatttcatc caccccataa
     2041 cctggtacgg cgccgttgac atcgccgacc gccaatccag ccccggtggc gtaaagctcg
     2101 ttccagtaag agccatccgt ggcgcctaga aactgggaga cctgctcaga tatttgcggg
     2161 ttggattggt gatcatagag atgcaggtat agatagatcc ctcgccaatt tccagaggag
     2221 tcatgccgcc agtcggagta gatggcaaac tcatcgttat tggaatcttg ttccggaagg
     2281 atatccccga cggcgatgcg caaggtgccg ccaaaaggaa agccatgcca gatatcaccc
     2341 cccgcagtat ccatggcgac ttcgttgtaa gaaatctcgg ttagctctcc ggtccaggcg
     2401 ccgccggcat cctttgcgac ctggtaaacg cgcaaggtta atttgcgctg cgcggcggcc
     2461 gaatccagca cggaagcaag gccaatctcg tccacaccat cgccatccac atccccggcg
     2521 gcaaccctgg gccagccggc tgcaatcgag gcggacaagg cggcaatttt gtgcagccgg
     2581 aaaccagact ggacatcata gatctcgacc atgatctgat cggtgggatc ggcataggcc
     2641 aggacgacct gctggcggcc atcaccggtg aaatagccgg tggcaagatc gaggtaggaa
     2701 ttttccagcg ggatggcggc gggcaaaggt ttggtgatgg tgcctgtgtc gaatacggtg
     2761 tgagacacat ccagtcctgg catgggtgtc cagggagcgg cgttcaggct gtactgcagg
     2821 ctgccgccgc tgagctgggt gaacaggaaa gcgccagcgg ggctgtctcc agccgcgatc
     2881 ccggcatcca gctccccgcc ggtaacggct gtgctgttcc aacccgtcca ggctgagccg
     2941 ttgaacaagt tttcccagat gcgattttcc gtaccggcga gatagatgtt cacctgacca
     3001 tcagcaggcg atgtgagcac cgggacggcg ttccaagcca ccccctgagg ggattccagg
     3061 cgctgccaag cggcccaggt gcccgcatac tcgcggtacc agacggcgcc atccagaccc
     3121 aaggcgtata cc
//
LOCUS       DATMXK010000217         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_128676, whole genome shotgun sequence.
ACCESSION   DATMXK010000217 DATMXK010000000
VERSION     DATMXK010000217.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128676"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..818
                     /locus_tag="VN363_04020"
     CDS             <1..818
                     /locus_tag="VN363_04020"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWQ83706.1"
                     /translation="PGADRPVVSSQQPVVSPLYDTLATSDVLLGLAARLGGKLAAALP
                     WPDEASYLEETVSELHGSSLGAYGARTPSGFWALWRQYGGWWSEKQIRREPELTDIVQ
                     KPLPIAAPQFEGDPQDYPFHLYPYSSITLSDGRGANLPWLQETPDPMTTVQWGMWVEI
                     NPETAEGLAISDNDVVQVESPYGSMEASVVIYPGIRPDMVAIPVGQGHQDYGRFAQAV
                     MGSNPMALVAPVTDPGTGALAWGATRVRLKPTGRKQVLARLESLDGNGRESLG"
     gene            796..1515
                     /locus_tag="VN363_04025"
     CDS             796..1515
                     /locus_tag="VN363_04025"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560859.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ83707.1"
                     /translation="MDANLSVETSRRWVLVVDLDRCTGCQACVVACHAENNVPIVGEK
                     QAGYGRWMHWIIIQRYWEGEYPNVKARFMPVMCQQCTEAPCEPVCPVFATYHNEEGLN
                     AQIYNRCIGTRFCGSACPYKVRTYNWYAPEFPEPLTEQLNPDVTVRTRGIMEKCTFCI
                     QRIRRIEIDAHAAGRIPADGEIQPACVQTCPSNVLFFGNLDDPDSQVSQLARGRRSFR
                     LLPELGTEPGVIYLKGGETNV"
     gene            1508..2926
                     /gene="nrfD"
                     /locus_tag="VN363_04030"
     CDS             1508..2926
                     /gene="nrfD"
                     /locus_tag="VN363_04030"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWQ83708.1"
                     /translation="MSEPDYTAPEMAQTTPYDERLLLAQRTDSEINQAVLQPMMQTGK
                     GFWALVTILSLIVAWGMYAWGYLIYWGIGVTGKNRPVYWTLFITTFVFWVGISHAGTM
                     ISAVLRLLKADWRRPITRGAEAMTAFALLMAALYPLIHLGRIWKFYWMIPYPNNRLMW
                     PNFQSPLMWDLVAIFTYLIGSSLYLYLALIPDMAMARDHTGSWRYKLYRVLALGWRGT
                     EGEWHKLHRALSIFSVAIIPIFLSVHSIVSWDFAVTIQPRWHSTIFAPYFVIGAVYSG
                     LAALASILVVVRRSMRLQEFLRAEHFDALGKLVMIAGMAWVYFWFAGFIVEWYGNEPV
                     VRELIREEVAGKLAPFFYLQMSANLIPIVLLVFKRVRTTPALLLAATLLINVGMFTER
                     ALIVIGSLQRNDIPFNWGTYRPTWVEVSIVAMTFAGFALLYTLFSRIIPFVPVWEIKE
                     GRLRYTMRRIGRLLVPTAAELD"
     gene            2984..3508
                     /locus_tag="VN363_04035"
     CDS             2984..3508
                     /locus_tag="VN363_04035"
                     /inference="COORDINATES: protein motif:HMM:NF023249.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="quinol:electron acceptor oxidoreductase subunit
                     ActD"
                     /protein_id="HWQ83709.1"
                     /translation="MTESMTLMALFHESTDTAEVIDQLHNLGIPDDKIVVITGVPYPE
                     GALGRHREWLTLPYIVLAGAIAGMFVGLFLAVITPHLYRLDVGGHPPVGFPPAAVIVF
                     VFTMMATIISTFLGVVWEMNFPRFGPAPYHKLVTDGYLALILDVPTSLEDDVMLTLET
                     HHAHHIGEPEWMPL"
BASE COUNT          728 a          952 c          995 g          854 t
ORIGIN      
        1 cacctggcgc tgatcgaccg gtggtaagca gccagcagcc agtagtgagc ccgctttacg
       61 atacgcttgc cacctccgat gtcttgctcg gattggctgc tcgattagga ggcaagcttg
      121 ccgctgcctt accctggccg gatgaggcat cctatctgga agagactgtg agtgagctgc
      181 acggctctag ccttggcgcg tatggcgccc gaacgccatc cgggttctgg gcgctttggc
      241 ggcagtatgg aggctggtgg tccgaaaaac aaatccgccg ggagcccgaa ctgacggaca
      301 ttgtgcagaa acctctgccg attgccgctc cacaattcga aggtgacccg caagactatc
      361 ctttccacct ttacccctat tcctcgatca ccctttctga cgggcgtggc gcaaatctac
      421 cctggctaca agaaacaccc gaccccatga cgaccgtgca gtggggaatg tgggtggaaa
      481 taaatcctga aactgctgaa ggattggcta taagcgataa cgatgttgtg caggtggaat
      541 cgccatacgg gtcgatggaa gcgtcagtcg tcatctaccc aggtattcga ccggatatgg
      601 tggccatccc ggtggggcaa ggacaccagg attatggtcg ctttgcccag gcggtaatgg
      661 gaagcaaccc aatggcgctg gtggctcccg tcacagaccc gggtacaggt gcattggcct
      721 ggggagccac acgtgtgcgg ctgaaaccga ccggaaggaa acaagtcttg gcgagattgg
      781 agagcctgga tggtaatgga cgcgaatctc tcggttgaaa cgtcccggcg gtgggtgctg
      841 gttgtcgatt tggatcgctg caccggctgc caggcatgcg tggtcgcctg ccacgccgag
      901 aataacgtac caatagtcgg cgagaagcag gcaggttacg gacgctggat gcactggatc
      961 atcatccaac gctactggga gggcgaatat ccaaatgtca aggcgcgctt catgccggtg
     1021 atgtgccagc agtgcaccga ggctccttgc gagccagtct gcccggtatt tgctacttac
     1081 cataacgaag aaggcctgaa cgcccagatc tataaccgct gcatcggcac gcgcttctgc
     1141 ggcagcgcct gtccttacaa agtccgcacc tataactggt acgcgccaga atttccggaa
     1201 ccgttgaccg agcagcttaa cccggatgtg accgtccgga cgcgcggcat catggagaaa
     1261 tgcacctttt gcatccagcg tatccgtcgt atagaaatcg atgcacatgc tgctggacgc
     1321 atccctgccg atggtgagat ccagccggcc tgtgtccaaa cttgcccctc aaatgtgttg
     1381 ttcttcggca atctggacga tccagacagt caggtcagcc aactggcacg cggccgccgc
     1441 tcttttcgcc tcttacccga attgggcacc gagccgggag ttatatacct caaaggaggc
     1501 gagacgaatg tctgagcccg attacaccgc acctgaaatg gcgcaaacca cgccttacga
     1561 tgaacgttta ttactggctc aacgcaccga ttcggagatt aaccaggccg tgctccagcc
     1621 aatgatgcaa accgggaagg gattctgggc tctggtaacc atcctgagcc tgattgtagc
     1681 ctgggggatg tatgcctggg gttatctcat ctactggggt attggcgtca ccgggaaaaa
     1741 ccgcccggtc tactggacct tattcatcac tacatttgtc ttctgggtgg gcatcagcca
     1801 cgctggaacg atgatctcgg ctgtgttgcg cttgcttaag gccgactggc ggcggccgat
     1861 cactcgcggg gctgaagcta tgactgcctt cgcgctgctg atggctgcgc tgtaccccct
     1921 gattcacctg gggcggatct ggaagtttta ctggatgatt ccttacccta ataaccgtct
     1981 catgtggccc aactttcaat ctccactgat gtgggatctg gtcgctatct ttacttacct
     2041 gatcggctca tcactatacc tgtacctggc gctcatcccg gatatggcta tggcgcgcga
     2101 ccacaccggt agctggcgat acaaactata ccgggtgttg gcattaggct ggcgcggcac
     2161 tgaaggggag tggcacaaac ttcaccgcgc attaagcatt ttctcggttg ccattatccc
     2221 gatctttctc tcagtacact cgatcgtatc ctgggatttt gctgtgacca ttcagccgcg
     2281 ctggcacagt accatctttg cgccctactt cgtcattggt gctgtctatt ctggcctcgc
     2341 cgcgttggcc agcatcctgg tggttgtgcg gcgcagcatg cgcttacagg agttcctacg
     2401 tgccgagcat ttcgacgccc tgggcaaact ggtcatgata gccggcatgg cctgggttta
     2461 tttctggttt gctggattta ttgtcgagtg gtatggaaac gagccggttg tgcgcgagct
     2521 gatccgggag gaggtagctg gcaaactggc accatttttc tatttgcaaa tgagcgcgaa
     2581 cctgatccca attgtgctgt tggtcttcaa acgcgtccgt acgactccag cgttgttatt
     2641 ggcagccaca ctgctgatca atgtaggcat gtttaccgag cgcgccctga tcgtcatcgg
     2701 cagcttgcag cgaaatgata tacctttcaa ttggggtact taccgcccta catgggtgga
     2761 ggtcagtatt gtcgccatga cctttgctgg ttttgccttg ttgtacactc tattttcgcg
     2821 catcatccca ttcgttccgg tatgggagat taaagagggt cgcttgcgtt acaccatgag
     2881 gcggattggg cgtctgctgg tgccgacggc ggctgaatta gactgaaaat tcgctgctcg
     2941 cgaaatgaga aaatgactgc taatcgtaat cggcggacaa aagatgactg aatcaatgac
     3001 cctgatggcc ctctttcacg agagcaccga tacggctgaa gtaattgacc aattacataa
     3061 cctgggcatt ccggatgaca agattgtagt cattaccggc gtgccatacc cggagggtgc
     3121 tttggggcgg catcgcgagt ggctaacctt accctacatt gtattggcgg gagctatagc
     3181 aggaatgttt gttggattgt tcttagcagt gatcactccg cacctctatc gtctggatgt
     3241 gggtgggcat cctcctgtgg gtttccctcc ggccgcagtg attgtttttg tatttactat
     3301 gatggccacc atcatctcga cttttttggg ggtagtgtgg gagatgaact ttcctcgttt
     3361 tgggccagcg ccctatcata aactcgttac agacggctat ctggctctaa tcctggatgt
     3421 cccaacgtca ctggaagacg atgtgatgct gacgctggaa actcatcatg cgcaccatat
     3481 cggtgagcct gaatggatgc ccttatgacc gctcgcctgc gtcgtgggc
//
LOCUS       DATMXK010000218         6650 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12650, whole genome shotgun sequence.
ACCESSION   DATMXK010000218 DATMXK010000000
VERSION     DATMXK010000218.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6650)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6650)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6650
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12650"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..189
                     /locus_tag="VN363_04040"
     CDS             <1..189
                     /locus_tag="VN363_04040"
                     /EC_number="2.5.1.17"
                     /inference="COORDINATES: protein motif:HMM:NF014033.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008817 - corrinoid adenosyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_process: GO:0009235 - cobalamin metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP:cob(I)alamin adenosyltransferase"
                     /protein_id="HWQ83710.1"
                     /translation="ALDLARTIVRRAERNVAQLVHLGELENHECLRYLNRLSSLCFVL
                     ELLENQASGTSLPTLAKQ"
     gene            230..976
                     /locus_tag="VN363_04045"
     CDS             230..976
                     /locus_tag="VN363_04045"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF554 domain-containing protein"
                     /protein_id="HWQ83711.1"
                     /translation="MTGTIINVAAIILGGGLGLLFGARFPERVRQTVLAGMGLFVAIL
                     GIQMTFDTQQPLVIAGSLLIGGLLGEWWQIEDRLRKLGAGLEERLTGKKSSQLTPVEN
                     PESNHGSRFVRGFLAASLVFAIGPIAILGSIQDGLTGDYSLLAIKSILDAFGALAFAS
                     SLGVGVLFSSLVILVYQGGISLLANQAQAILTTPMTNEMTATGGVLLLGLAISNLLEI
                     KPIRVGNFLPALVVAPIIVAILSALGIPLN"
     gene            complement(1030..1779)
                     /locus_tag="VN363_04050"
     CDS             complement(1030..1779)
                     /locus_tag="VN363_04050"
                     /inference="COORDINATES: protein motif:HMM:NF024853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWQ83712.1"
                     /translation="MEKKTLSKRETIREQRRKKEQRNKILIIGAATIAALALGAIIFV
                     PNLINANTPVGEIVQITPVAHPQEDGRAMGPVDAKVVVEVFEDFQCVACEGYTQSIEP
                     LVLQNYVETGKIRYEFRHYPFLDDRSSVKESDQAANASMCAADQGRFWDFHDILFANF
                     GEGAGAYTDNRLEAYAEAIGLDMNAFNTCYQNEQFDSVIKQDMADATSLGVTGTPTVF
                     VNGKLTGREGLVATYDEISQMIEQELAASGS"
     gene            complement(1820..2722)
                     /locus_tag="VN363_04055"
     CDS             complement(1820..2722)
                     /locus_tag="VN363_04055"
                     /inference="COORDINATES: protein motif:HMM:NF023568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipase"
                     /protein_id="HWQ83713.1"
                     /translation="MKVVDETWQASRGAVVFGRSWLPDEEPRAALILIHGLGEHSGRY
                     WHVAEHFARAGYLVQSMDLRGHGKTPGQRGHVPSIAIILEDIDLLLEKVAHAYPGLEC
                     FLYGHSLGALLAVNYALRYLRPLTGVVATGLATSTPLMDQTTKITLARVLGSILPRIT
                     LATGVDPNTICRDPQVVQAYIADSLVHDRSSLGLGKGMIDMVDFIKAHAQEFKHPLLL
                     CHGTRDQLAYPQGSQEFARLVPRNVTLKLWEGLFHEVHNEPEKELVLEYIRGWMDQQL
                     ILQGGEKHSSNINPPPEFVAGQSE"
     gene            complement(2784..3647)
                     /locus_tag="VN363_04060"
     CDS             complement(2784..3647)
                     /locus_tag="VN363_04060"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ83714.1"
                     /translation="MLSQLNLAIRDALVSDQQRIANLIHFEGHTHRNLDWRSPLDWLG
                     FKPFLIAEQEGQVVGALACPPDPADVAWIRLFSSATYLPILPVWQQLWEAVLEQVAET
                     QSISRLVSLPLNHWYEELLLKSNFKVTQTVVMLRIDLPSEPVDYLPPPDGVTLRSMSL
                     DDLPAVQIVDEAAFQPIWRNSLASLEIAFRQANMATVAELDGEIIAYQITTRTESRVH
                     LARLAVHPRQQGRHIGKSLLLDLLSQYQRRGYQRLTVNTQNDNRGSLRLYKNAGFSPT
                     GEEYPIYEYVP"
     gene            complement(3666..4208)
                     /locus_tag="VN363_04065"
     CDS             complement(3666..4208)
                     /locus_tag="VN363_04065"
                     /inference="COORDINATES: protein motif:HMM:NF026288.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83715.1"
                     /translation="MVDSTSPRSISTEGQTAYLRKDYLAAAGRFNAAAAAYIQVGDAL
                     MAAEMHNNASVAYLQAGNAAAALDEVGDTPEIFAAAGDLRRQAIAVGNQAAALEGLKR
                     VPEALVAYELCADLFKHAGETDLRVHAMQARSALELRSGHQLQALATMKSGLDDLKKP
                     SPKQRFLRRLLDIPFSMWKK"
     gene            complement(4245..4919)
                     /locus_tag="VN363_04070"
     CDS             complement(4245..4919)
                     /locus_tag="VN363_04070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83716.1"
                     /translation="MKPINTHTIRAKKLGVLIRDARMTAGRGVPDCAAAMGVGVEVYE
                     AFETGDQSPSLPELEFLAYFLDIPLEHFWGETTLSARLEKNRVDVQRLALVRQKVIGV
                     LLRKARLEAKKTLEDVVQTAGIPLEDLEAYEYGELPVPLPLLEQLVGILNRDLREFQD
                     RKGPVGVWFIQQRSLQDFKELPPELQEFVSRPINRPYLELAQRLSEMSVDKLRGVAEG
                     LLEITL"
     gene            complement(4932..5621)
                     /locus_tag="VN363_04075"
     CDS             complement(4932..5621)
                     /locus_tag="VN363_04075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559801.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83717.1"
                     /translation="MTQSILIVDDEPRYLRLMEANLVTEGFHVYQATNGQEAVDVVAE
                     KHPDLVFLDIMMPVLDGFGALERIREFSNVPIIIVTARGSENDRVRGLDLGADDYIVK
                     PFSATELLARARAVLRRAQVSGGSFQQSIFTHGNLRIDFARAEVYKGDKMVFLSATEY
                     RLLLQFAHNLGSVLTSDQLLTDVWGPEYKDDKEILWVSISRLRQKLEADAKNPRHIVT
                     RSGLGYTMPQM"
     gene            complement(5618..>6650)
                     /locus_tag="VN363_04080"
     CDS             complement(5618..>6650)
                     /locus_tag="VN363_04080"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWQ83718.1"
                     /translation="ADLAWGEAVAVDAFHSRQTILRREELNDEDPTNRDRTSLRYYLG
                     IPLNLGDEVVGSLVFVRFGGPPYSPDHILLAEFIAVHIAQLLSRHQLVERIARLEAKR
                     QLDNLQDGFMAMVSHELLTPLGFIKGYATTLLRQDTEWDPVTQREFLNIIDEESDRLH
                     ELIDNLMDSSRLQAGTLRMSFQPLKLDTFLRDVALRASSRNETLSIELQLKVTGLQVI
                     ADPTRMAQIFENLLGNAVKYAPGAPITIGLDLDNKMAHITVNDQGPGIAPENLVHLFK
                     RFYRIPGPNTSIRGTGLGLYICKKIIQAHQGDMDVESEVGKGTTFNIYLPIENMAMGQ
                     SSITQEVNS"
BASE COUNT         1487 a         1853 c         1777 g         1533 t
ORIGIN      
        1 gctctggacc tggcacgtac gattgtgcgc cgtgccgagc ggaatgtggc tcagctggtc
       61 cacctggggg agctcgaaaa ccacgagtgc ctgcgctatc tgaaccggct ttcttccctg
      121 tgttttgtgc tcgagctgct cgaaaaccag gccagtggga ccagcctgcc caccctggca
      181 aaacaataaa cctggctttt ccccatccta aaactgaggt ttttctactg tgactggaac
      241 catcatcaat gttgctgcca ttattctcgg aggtgggctt ggtttattgt ttggcgcccg
      301 cttccccgag cgggttcgcc agactgtcct ggcagggatg ggattatttg tagctatcct
      361 gggcatccag atgacgttcg acacacagca gcctctggtc atcgctggct cgctgctgat
      421 cggcggcttg ttgggggagt ggtggcagat cgaagaccgt ttgcgcaagc tgggcgccgg
      481 gttggaagag cgtctgacgg gaaagaaatc cagccagcta acaccagttg agaatccgga
      541 aagcaaccac ggcagccggt tcgtgcgcgg tttcctcgcc gcctcgctgg tgtttgccat
      601 tggcccgatc gccatcctcg gctccatcca ggatggcctg actggtgatt acagcctgct
      661 ggcgatcaaa tccatcctgg atgcgtttgg agcgctggca tttgcttctt cgctgggggt
      721 tggggtattg ttctccagcc tggttattct cgtctaccag ggtgggatca gcctgctggc
      781 aaaccaggcc caggccatcc tcaccacgcc catgaccaat gaaatgacag ccaccggcgg
      841 cgtgctcttg ctgggcctgg cgatcagcaa cctgcttgag atcaaaccaa tccgggtggg
      901 aaacttcctg ccggcgctgg tggtcgcccc gatcattgtc gcgatcctct ccgcgttggg
      961 catccccctg aattaaataa aactgagccg gcagtttacc ggcccaggct gtatcaaatc
     1021 agcgccagat caggaaccgc tggctgctaa ttcctgttcg atcatctggc tgatttcatc
     1081 gtatgtagcg actaaacctt cccgaccggt gagcttgccg ttgacaaaaa ccgtcggggt
     1141 tccagttaca ccaagggacg tcgcatccgc catatcctgc ttgatcacac tgtcaaactg
     1201 ctcgttctgg tagcaggtat taaaggcatt catatccaga ccaatcgcct cagcgtaggc
     1261 ttccaggcgg ttatctgtgt aagctccagc accttcacca aaattggcga acagaatatc
     1321 atggaaatcc caaaaacgac cctggtccgc agcacacatg ctggcatttg cggcctgatc
     1381 cgactctttc acgctggaac gatcgtccag gaagggataa tggcgaaact cataccggat
     1441 cttaccagtt tcgacatagt tctggagcac taaaggctct atcgattgtg tgtatccttc
     1501 acaggccaca cattgaaaat cttcgaatac ttccacgacg actttcgcat cgacaggtcc
     1561 catcgctcgc ccatcttctt gaggatgggc taccggggtg atctggacga tctcgccaac
     1621 cggcgtattg gcattgatca agttgggtac gaatatgatc gctcccagcg ccagggcagc
     1681 aattgtggca gcgccgatga tcaggatctt attgcgctgc tcctttttcc ggcgctgctc
     1741 gcgaatagtt tcacgctttg aaagggtctt cttctccata aatcctcctg cttagattag
     1801 taaggaattt tgccataccc tattcgcttt gtccagccac gaactctggc gggggattaa
     1861 tattggaaga gtgtttttcg cctccctgca ggatcagctg ctggtccatc caaccccgaa
     1921 tatactccag gaccagctct ttttcaggct cgttatgcac ctcgtgaaat aacccttccc
     1981 acagcttgag ggtcacattt ctggggacaa gtctggcaaa ttcctgactg ccctgaggat
     2041 aagccagctg gtcgcgggtg ccatggcaaa ggagcaaggg atgtttaaac tcctgagcat
     2101 gtgcctttat gaaatcgacc atatcgatca tcccttttcc taatcccagg ctggaacggt
     2161 catgcaccag tgaatcggca atataggcct ggacgacctg cggatcacgg catatcgtat
     2221 tcggatccac accagtcgcc agggtaatcc tcgggaggat tgagcccaaa actcgcgcca
     2281 gggtgatttt tgttgtttgg tccatcagag gagtggatgt agccaggcca gtcgcaacca
     2341 cacctgtcaa aggacggagg taacgcagag cataattgac cgccagaagt gcgcccaggc
     2401 tgtgcccata caaaaagcac tccaaaccgg gataggcgtg ggcgactttc tccagtaata
     2461 ggtctatatc ttctaagatg atcgctattg aagggacatg gccgcgctgc ccgggggttt
     2521 taccatgacc gcgtaaatcc atgctttgta caagataacc tgcccgggca aaatgctcag
     2581 ccacatgcca gtagcgtcca ctgtgctcgc ccagaccatg gattaaaata agcgcagccc
     2641 gcggttcttc atccggcagc cagctgcgcc cgaataccac cgcgccacgg ctcgcctgcc
     2701 aggtttcatc aacaactttc atgctcaagc tcctcaatgc gatctcggct tcaggcagtc
     2761 ctcagcgccg gatatttcat tcttcagggg acatattcat aaatcgggta ttcttccccc
     2821 gtcgggctga agcccgcatt tttatacaac ctcaatgaac ctcgattgtc gttttgggtg
     2881 ttgacagtca gacgctggta tccccggcgc tgatactggg agagcaaatc gagcagcagg
     2941 cttttaccaa tatggcggcc ctgctggcga ggatgcacag ccaggcgcgc cagatgaacc
     3001 cgcgattcgg tgcgggtagt gatctggtag gcgataatct ctccatccag ctcagccacg
     3061 gtggccatgt tcgcctgccg gaaagcaatc tccagcgagg cgagggaatt gcgccagatg
     3121 ggctggaagg cggcttcatc aacgatctgt acagccggta aatcatccag gctcatcgaa
     3181 cgcagagtga ccccatcggg tggaggcagg taatcaactg gttcggacgg gagatcaatg
     3241 cgcagcatta ccactgtctg ggtaactttg aaattgcttt tgagcaggag ctcctcatac
     3301 cagtgattca aaggtaatga aaccaggcga gaaatactct gcgtttctgc gacctgctcg
     3361 aggactgcct cccagagctg ctgccatacg ggcagaattg gcaggtaggt cgcgctggaa
     3421 aataaccgga tccaggcgac atcggccgga tcgggcgggc aggccagcgc tcccactacc
     3481 tgcccctcct gttcggcaat caagaatggc ttaaagccta accagtccag gggcgagcgc
     3541 caatccaggt tgcggtgggt atggccctca aaatggatca gattcgcaat ccgctgctgg
     3601 tcgctgacaa gagcgtctcg tatagccagg tttaattggg agagcatacc ttgttcaata
     3661 ccttgttatt ttttccacat gctgaaagga atatccaata agcggcgtaa gaaacgttgt
     3721 ttcggtgaag gtttttttag atcgtcaagg ccagatttca ttgtcgccag cgcctgcagc
     3781 tggtggccgg atcgaagctc taaagcagac ctcgcctgca tggcatgtac gcgcaggtcg
     3841 gtttctcctg catgtttgaa taaatccgcg caaagctcat aggccaccag ggcttctggc
     3901 acgcgcttta aaccttccag ggcggctgcc tggttgccga ctgcgattgc ctggcggcgc
     3961 aggtctccgg cggcggcgaa gatttccggt gtatccccca cctcatctaa ggcagcggcg
     4021 gcattgccag cctgcagata agccacgctg gcattattgt gcatttcggc agccatcagc
     4081 gcgtccccaa cctggatata cgctgccgcg gctgcgttaa atcgcccggc agccgccaga
     4141 taatccttgc gcagataagc tgtctgaccc tctgtggaaa tcgagcgcgg cgaagtagaa
     4201 tcaaccatcc gaaattctcc gagtcgcaag gaaatcgcta aggatcagag ggttatctcc
     4261 aataaccctt cagcaacacc gcgcagctta tcgaccgaca tctcgctcag tcgctgcgcc
     4321 aattccagat aagggcgatt aatcggcctg gagacgaact cctgcagctc gggaggcagt
     4381 tccttgaaat cctgcaggga gcgctgctgg ataaaccaga cccctaccgg ccctttgcga
     4441 tcctggaatt cacgcagatc acgattcaag atcccaacca gctgctccaa taatgggagc
     4501 ggaacgggaa gctcaccgta ctcataggct tcgaggtcct ctaacggtat cccggcagtc
     4561 tggacgacat cctcgagggt tttttttgct tccaggcgcg cttttcgcag cagcacgccg
     4621 atcaccttct gccgcaccag ggccaacctc tgcacatcaa cccgattctt ttccagccgg
     4681 gcgctgagcg tcgtttcgcc ccagaaatgc tccaggggga tatccaggaa ataagcgagg
     4741 aactcgagct ccggcaagga tggggattga tctcctgtct caaacgcctc gtagacttcg
     4801 acgcccacac ccattgccgc ggcgcagtcg ggaacgcccc gtccggcagt catccgggca
     4861 tcgcgaatta atacacctaa tttttttgca cggatcgtat gtgtgtttat tggtttcatg
     4921 cgctcagttt cctacatttg cggcatggta tagcccaaac ccgagcgggt gacaatatgg
     4981 cgcgggtttt tggcatccgc ctccagcttt tggcgcaggc gggaaatact gacccacagg
     5041 atctctttat catctttgta ttccggtccc catacgtccg tcaggagctg gtcggaggtg
     5101 agcacgctgc ccaaattgtg ggcaaattgg agcagcaggc ggtattctgt cgccgagaga
     5161 aagaccattt tatcgccttt atagacttca gcccgggcaa aatcgatccg caggttccca
     5221 tgtgtaaaaa tcgactgctg gaagctgcca cccgaaacct gcgcccggcg caatacagct
     5281 cgggcacgag ccaacaattc tgtggcagag aagggtttaa caatataatc atcggcaccc
     5341 agatcgaggc cgcggacgcg atcgttctca ctgccgcggg cagtaacgat gataatgggc
     5401 acattcgaga actcgcggat gcgttccagg gctccaaaac catccaggac tggcatcata
     5461 atatccagaa agaccaggtc gggatgtttt tctgcgacca cgtccaccgc ctcctgcccg
     5521 ttggttgcct ggtagacatg gaaaccctcg gtcaccagat ttgcttccat caggcgtaaa
     5581 tagcgcggct catcatcgac aatcaaaata ctttgcgtca tgagttcacc tcctgagtga
     5641 tggaactctg tcccatggcc atattttcaa tgggcaaata aatattgaaa gttgtcccct
     5701 tgcccacctc ggactctaca tccatatcac cttgatgggc ctggatgatc tttttgcaaa
     5761 tatataatcc caggccggtg ccgcggattg aagtattggg accaggaatg cggtaaaacc
     5821 gcttaaagag atgcaccagg ttttctggag caatcccggg tccctggtcg ttgaccgtaa
     5881 tgtgcgccat tttattgtcg aggtccagac caatcgtaat gggcgcacct ggggcgtatt
     5941 ttacagcatt gccgagcaag ttttcgaaga tctgggccat gcgggttgga tcagcgatta
     6001 cctgcagtcc ggtcactttt agctgcagct caatgctgag tgtttcattg cgagagctgg
     6061 cgcgtaatgc gacatcgcgc aaaaacgtat ccaatttgag cggttggaag ctcatgcgca
     6121 gcgtgccggc ttgcaagcga gaagaatcca tgagattgtc gatcagctcg tgcaggcggt
     6181 cgctttcttc gtcgatgata ttaaggaatt ctcgctgtgt aacagggtcc cactctgtat
     6241 cctggcgcaa gagagtggtg gcatagcctt tgataaaacc cagcggagtg agcagctcgt
     6301 gggaaaccat tgccataaac ccatcttgaa gattatcgag ctggcgtttg gcttccaggc
     6361 gggcaatccg ttcgacaagc tggtgacggc tgagcagctg ggcaatgtga acagcgataa
     6421 attcggccag caggatgtga tcgggcgaat aagggggtcc gccaaagcgg acaaacacca
     6481 gagagccgac gacttcatct ccaagattta gcggtatccc cagatagtag cgcaggctgg
     6541 ttcgatcccg gttggtggga tcttcatcat tgagctcttc acgccgcagg attgtctggc
     6601 ggctgtggaa agcatccact gccaccgctt caccccaggc aagatcggct
//
LOCUS       DATMXK010000219         4890 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_1202, whole genome shotgun sequence.
ACCESSION   DATMXK010000219 DATMXK010000000
VERSION     DATMXK010000219.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4890)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4890)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4890
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1202"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..462
                     /locus_tag="VN363_04085"
     CDS             <1..462
                     /locus_tag="VN363_04085"
                     /inference="COORDINATES: protein motif:HMM:NF015213.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_component: GO:0033573 - high-affinity iron permease
                     complex [Evidence IEA];
                     GO_function: GO:0005381 - iron ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0034755 - iron ion transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FTR1 family protein"
                     /protein_id="HWQ83719.1"
                     /translation="FLAVGREGFELVLFLVAARITAGGMQTILGAALGLAAAIVLGWM
                     LFATSKRLSLRQFFNVTNILLVFFAAGLVAHGVHEFNEAGLIPAIIEHVWDLNPVLDE
                     KGVMGQLLTALFGYNANPSLSEVIAYAAYFVGLGLAWKRLARPAVAAQAAS"
     gene            complement(599..2371)
                     /locus_tag="VN363_04090"
     CDS             complement(599..2371)
                     /locus_tag="VN363_04090"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHRD domain-containing protein"
                     /protein_id="HWQ83720.1"
                     /translation="MIKKVRPLTAAVIITLLTLAVAVTGLAQARLPLEDPIPEPIKTG
                     PLEISLEQVAAGMTAPNWGTFAPGCSSLSSRLFLVDQVGILWNLNLATGAKSDMLDVS
                     ARLVPLGAFGPGTFDERGFLGVAFHPDYAANGLLYTYTSEPVSGPADFSTIPPGGIPN
                     NQIVVAEWNTPNPCSAASVVNPASRRELMRIDDPQFNHNAGALVFGPDGMLYIAIGDG
                     GAADDQGFGHVEGGNAQNPSNIFGNILRIDPTGSNSANGKYGIPADNPFVGMPGFLGE
                     IFAYGFRNPFRFSFDSLSGEMYIGDVGQNDIEEVSLGAAGGNYGWNIKEGSFCFDPNG
                     TSPGFVFDCAPGDAPDGLIEPIAEYDHDEGISVIGGFVYRGSRIPQLKGRYVFGDFND
                     PSGGGRLFYLQQNNRILEFQIAGQDSFGLSLLGFGQDADGEIYALANAGGVPFGETGV
                     VLRIGPSSGRTDNFQAHLSGAEENPPVDTQAQGEATFMFNEDTTQLDFNLIAANIDNV
                     VASHIHCAPAGVNGSVGVTLFSGGPVTPNGPLASGSVSAPDAGNRCSWTTLQDVLTAI
                     ETGNAYANVHTTAFPGGEIRGQIR"
     gene            complement(2707..3252)
                     /gene="msrA"
                     /locus_tag="VN363_04095"
     CDS             complement(2707..3252)
                     /gene="msrA"
                     /locus_tag="VN363_04095"
                     /EC_number="1.8.4.11"
                     /inference="COORDINATES: protein motif:HMM:NF013766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008113 - peptide-methionine (S)-S-oxide
                     reductase activity [Evidence IEA];
                     GO_function: GO:0033744 -
                     L-methionine:thioredoxin-disulfide S-oxidoreductase
                     activity [Evidence IEA];
                     GO_process: GO:0006979 - response to oxidative stress
                     [Evidence IEA];
                     GO_process: GO:0030091 - protein repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide-methionine (S)-S-oxide reductase MsrA"
                     /protein_id="HWQ83721.1"
                     /translation="MQSNPTQLATLAGGCFWCLEAVFEQLKGVEKSVSGYAGGTQANP
                     SYAEVCTGRTGHAEVVQLTFDPAQVSYRDLLNVFFSIHDPTTLDRQGADVGSQYRSAI
                     YYHTPEQKAIAEETIQDLEARRLWSNPIVTEVVPLDVFYPAEDYHQGYFRAHPYQGYC
                     QMVIAPKVAKFRQHYLDKLKA"
     gene            3598..4632
                     /locus_tag="VN363_04100"
     CDS             3598..4632
                     /locus_tag="VN363_04100"
                     /EC_number="1.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)-dependent alcohol dehydrogenase"
                     /protein_id="HWQ83722.1"
                     /translation="MAQLTILGYGTKAKGQALEPFTYEPPRLGEQDVRVSVTHCGVCH
                     TDIQAIDDYYGITTFPFVPGHEIVGNVTETGPAVVELKIGDRVGIGWQGRSCGQCEWC
                     LQGETQLCMEIEKTTVMVPYGGFSASVTADFRFAHLLPASLPSETAAVLMCAGVTVYS
                     ALRTYLTRPLQKVAILGIGGLGHLAIQFAHAFGCEVTAISSSPEKKAQALGFGADHFI
                     HSAQLESMRAYNYYFDLLICTANHGINWSALLMLLKKRGRLVLVGFPDLELNSTNLVA
                     HELSMTGRLIGNPTAMREMLIFAQEHQITPMVELLPMSQVNEALRRVKENQARYRIVL
                     VNDTLAGGLK"
     gene            4647..4829
                     /locus_tag="VN363_04105"
     CDS             4647..4829
                     /locus_tag="VN363_04105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83723.1"
                     /translation="MRLPYLAPMEAAGREPRQVTAIRKLAYLLHPYYFSNPPDSVSPW
                     ELGKIDAAYLPPAPLR"
BASE COUNT         1006 a         1267 c         1581 g         1036 t
ORIGIN      
        1 tttctggcgg tcggccgcga agggttcgaa ttagtattgt ttttagtcgc cgccaggatt
       61 acagctgggg gaatgcagac tatcctgggc gcggctctgg gactggcggc tgcgatcgtc
      121 ctgggctgga tgctttttgc aaccagcaaa cgcttgagtc tgcgccagtt ctttaatgtg
      181 accaatatcc tgctggtatt ctttgcggcc ggattggtgg cgcacggcgt tcacgagttt
      241 aatgaagccg ggcttattcc ggcgatcatc gagcatgtgt gggatctcaa tcctgttttg
      301 gatgagaaag gtgtcatggg gcagctgctt acggcgctgt ttggctacaa cgccaatccg
      361 tcgctgagcg aagtcatcgc gtatgcggcc tatttcgttg ggcttgggct ggcatggaaa
      421 aggttagcgc ggccggccgt cgccgcgcag gcagcctcct gaagcattct cgatccaggc
      481 ttacggcagc tgaatgaatt cttatgctgc tgatcaaaag ggctgcccag ctctgtggac
      541 agccctttca gattccagct ttatgccggt gtgcctggca ggataaatcg ctgaggggtt
      601 acctgatctg gccgcggatc tcaccacctg gaaaggctgt ggtatgaaca ttggcgtaag
      661 cgttgccggt ctcgatggca gtcagaacat cctgcagggt ggtccagctg cagcggttgc
      721 cagcatcagg cgcgctgacc gagccgctcg ccagaggacc attcggtgtg accgggccgc
      781 ctgagaagag cgtgacacct accgagccgt tcacaccggc cggggcgcag tggatgtgag
      841 aggcaacaac attgtcgata ttggcggcga tcagattgaa gtccagctga gtggtatctt
      901 cattaaacat aaaggtggct tcgccctgag cctgggtatc caccggcggg ttttcttcgg
      961 cgccgctcag gtgggcctgg aagttatcgg tacgtccaga ggatggcccg atgcgcagga
     1021 cgactccggt ctcaccgaag ggcacgccgc cggcattcgc cagggcgtaa atttccccat
     1081 ctgcatcctg gccgaaacca agcaggctca ggccaaaact atcctggccg gcgatctgga
     1141 attccaggat gcggttgttc tgctgcaggt agaacagccg cccaccgccc gacggatcgt
     1201 tgaagtcgcc gaacacatag cgacccttaa gctgcgggat gcggctgccg cgatagacga
     1261 atccgccgat caccgagatt ccctcatcgt ggtcgtactc ggcgatgggt tcgatcaggc
     1321 cgtctggcgc atcgccggga gcgcagtcaa agacgaatcc ggggctggtc ccgttgggat
     1381 cgaagcagaa ggatccttcc ttgatattcc agccgtaatt cccgccggca gcacccaggc
     1441 tgacctcttc aatgtcgttc tgcccgacat cgccaatata catctcaccg ctgagcgagt
     1501 cgaacgagaa gcggaagggg ttacggaagc cgtaggcgaa gatctcgccg agaaagcccg
     1561 gcattcccac gaagggatta tcggcgggga tgccgtattt gccattggcc gaattggacc
     1621 cggtcgggtc gatgcgcaga atattgccga atatgttgct cgggttctgg gcattgccgc
     1681 cctccacatg cccgaagccc tggtcatcgg cggcgccgcc atcgccaatg gcaatgtaga
     1741 gcatcccatc cggaccgaat accagggcac cggcattatg gttgaactgg ggatcatcga
     1801 tccgcatcag ctcacggcgg gaggccggat tgacgaccga ggccgcgctg cagggattgg
     1861 gagtgttcca ttcagccacg acgatctggt tgttgggaat cccgccgggg gggatggtgg
     1921 agaaatcggc cggacctgaa accggctcgg aggtgtaggt gtagagcagg ccgttggcgg
     1981 cgtaatcggg atggaatgcg acgccgagaa agccgcgctc atcgaaagtg ccgggaccga
     2041 aggcgcccag cgggacgagg cgggcgctga catcgagcat atcgctctta gctccggtgg
     2101 caaggtttaa attccacaag attccgacct ggtcgacgag aaacaggcgg ctgcttaagc
     2161 tggagcagcc gggggcaaat gtcccccagt tgggagcggt catgccagcg gcaacctgct
     2221 ccaggctgat ctcaagggga ccggtcttaa tgggttcggg gatggggtct tcgagaggca
     2281 agcgggcctg ggcgagcccg gtaacggcaa ccgccagggt gagcagggtg atgataacgg
     2341 cggcggtaag tggtctgact ttcttgatca ttgggtttcc tccagtacta cttcaccaat
     2401 aagctgaacg taaacctgta aatctgccag atacacagca caaccttcgc attagaatac
     2461 gtgaggaaaa gcggactgga tttatcggat ttgaagtgag ggtacagcgg atttcgaacg
     2521 gattgaacgg attggtttat ggggaaatgg gctgcgaggg gagggagggt attcgactgc
     2581 gcagccaggt agcaaggctg ctccgctcag accgcggcaa gcgcgctgag cggagtgagc
     2641 gctctgtgcg aacgcagtcg aagcgtggcc gggtgcagtt gtgccaggat gatttggagg
     2701 cgtggattac gctttcaatt tatccaggta gtgctggcgg aatttggcga ctttgggggc
     2761 gatgaccatc tggcagtagc cctgataggg gtgggcgcgg aagtagccct ggtggtagtc
     2821 ttctgcggga taaaaaacat ccagcgggac gacctcggtg acgatgggat tggaccacag
     2881 gcgccgggct tcgaggtcct ggatggtttc ttcggcgatg gctttctgct cgggggtatg
     2941 gtagtagatc gccgagcggt actgcgagcc cacatcggcg ccctggcggt cgagggtggt
     3001 ggggtcgtgg atgctgaaga agacgttgag caggtcgcgg tagctgacct gggcggggtc
     3061 gaaggtgagc tggacgactt cagcgtggcc ggtgcgcccg gtacagactt ccgcataact
     3121 cgggttggct tgcgtgcctc cggcgtaccc cgagacggat ttttcgacgc ctttgagctg
     3181 ctcaaagacg gcttcgaggc accagaagca gccgccggcg agagtggcga gctgggttgg
     3241 gttggattgc atgggttgcc tcctgtgcgt tactcgtatg atggtggttc aaatcggata
     3301 gaaactggct gaatattatc acaaaatcca ctggcctggc atacggttgg acgggctcac
     3361 cattggcctg aggaagcgcg cgggtagagg gtggttgaaa tccagcgaat ttcgcttgac
     3421 tcgagctctg caaagaccta taatgaatca acgcagatgc attgcgtgtt ccaatcttat
     3481 tatcctctat caccccttga aaccgcagcc tgctgcggac ctgatcaagg ggtctttttt
     3541 tttgacggaa gcatggatca agatcgatcc accgaggtgg aaaggaaggt actaccaatg
     3601 gctcaattaa cgatcctggg atatggtacg aaagctaagg gtcaggcgct cgaaccattc
     3661 acttatgagc cgcccagact gggagagcag gatgtgcggg tctccgttac acattgcggc
     3721 gtatgccata cggatatcca ggccatcgac gattactacg ggatcactac ctttccgttt
     3781 gtgcccgggc atgagatcgt ggggaatgtg acggagacag gtccggcggt ggtcgagctg
     3841 aaaatcggag accgggtggg catcgggtgg cagggcaggt cgtgcggtca atgcgagtgg
     3901 tgtttgcagg gggagacaca gctgtgtatg gagatcgaaa aaacgaccgt gatggttcct
     3961 tatgggggat tctcggcgtc ggtgactgcc gatttccggt tcgcacacct gctcccggca
     4021 agtttgcctt cggagactgc cgcagtgctg atgtgcgctg gcgtgacggt ctattcggcc
     4081 ctgcgcacct acctcacgag acccctacaa aaggtcgcca tcctgggtat cggagggctc
     4141 ggccacctgg cgatccaatt tgcccatgcc ttcggctgcg aggttacagc tatctcctcc
     4201 tcgccggaaa agaaggcgca ggcgctcggg tttggcgcgg atcactttat ccattccgca
     4261 cagctggaga gcatgcgagc ctacaactat tattttgatc tgctgatctg taccgcaaat
     4321 catgggatca actggtcagc gctgctcatg ctattgaaga aaagggggag attggtcctg
     4381 gttgggttcc ccgacctgga gttaaattcg accaatcttg tagcccatga attatcgatg
     4441 accggccgcc tgatcggaaa tcccaccgcc atgcgagaga tgctcatatt cgcgcaggag
     4501 caccagatta cccccatggt cgaactgctg ccgatgtcac aggtaaacga ggctctgcgg
     4561 agagtaaaag agaaccaggc gcgctaccgg atcgtgctgg ttaatgacac gctcgccggt
     4621 ggtttgaagt agattgagca acattggtga gactcccata tcttgcacca atggaagctg
     4681 cagggcgcga gccgcgccag gtgacggcga tccgcaagct ggcgtacctg ctgcacccgt
     4741 attatttcag caacccgccg gattcggtct caccgtggga gctgggcaag atcgacgcgg
     4801 cgtatctgcc gccggctccg ctgcgctagg tggagaccct acaggtttta aagtattcgg
     4861 gtacggcggg aggaattccc gtgcggagag
//
LOCUS       DATMXK010000220         3659 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_79844, whole genome shotgun sequence.
ACCESSION   DATMXK010000220 DATMXK010000000
VERSION     DATMXK010000220.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3659)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3659)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3659
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_79844"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            22..258
                     /locus_tag="VN363_04110"
     CDS             22..258
                     /locus_tag="VN363_04110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83724.1"
                     /translation="MDAIEVQARLEASGKLIPIEFSWLGRTYFVSSIGRRWKDSQGQH
                     ILVQANGEQVYELLFQAGAEQWWLVKKPVFPSVA"
     gene            complement(336..2120)
                     /gene="metG"
                     /locus_tag="VN363_04115"
     CDS             complement(336..2120)
                     /gene="metG"
                     /locus_tag="VN363_04115"
                     /EC_number="6.1.1.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558838.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004825 - methionine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006431 - methionyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine--tRNA ligase"
                     /protein_id="HWQ83725.1"
                     /translation="MSEHILVAVAWPYANSLIHVGNLTGSSLPADIFARYQRLAGNQV
                     IMVSGTDSHGTPVTVRADAESSTPVAVYERFHAGFLDLYQKLGISFDLFTSTHTENHF
                     RVSQAIFLALRENGYLYTESQQQWFAPNQNRFLPDRYIEGTCYICGYANARGDQCDNC
                     GELLDATQLINPRSKIDGSTPDLRQTEHFYLDLGKLQPEIVEFLRQRESYLRPNVLRQ
                     SLGQIEANALHGRAITRDLDWGIPVPVEGWEGKRLYVWFEAVIGYLSAAIEWSHLDGS
                     KDGWHDWWTNPEMRSYYFIGKDNIPFHAVIWPGQLIGAGEAFSQIFEERPDAPLTLPF
                     DIPANEFMNLEGKKISGSRNWAVWGLDFLERYDPDPLRYYLTVNMPESKDTDWDWDGF
                     VHRNNDELVATWGNLANRVLSFAYRNWEGVIPEPGVLRSGDEAILAVVEAGFLECGEH
                     LKAVRLRAALSEAIRLASEVNKYLDTTAPWFEIKTDRAAAATTVYTALRCIDSLKTLL
                     APFLPFTSEQLNTYLGYQQPLFGEQYTEVVEDKLGTHQVLRYRADTARGSWEPSQLPA
                     GQKLVQPAPLFRKLEESVVAEERARLGQ"
     gene            complement(2465..2986)
                     /locus_tag="VN363_04120"
     CDS             complement(2465..2986)
                     /locus_tag="VN363_04120"
                     /inference="COORDINATES: protein motif:HMM:NF014657.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF177 domain-containing protein"
                     /protein_id="HWQ83726.1"
                     /translation="MNATNHLRLNVGFIIHETIGYIREFPLDLPFIHLPPDLDIEQFS
                     GVVRITRAQQGLVVQVQVQGETTAQCGRCLVDFSQQVSANFTDLYAFTPKLAAESGLV
                     LPENGQLDLEPSIREELLLSLPINPLCRPDCKGLCPVCGDNRNEVQCEHSAEVIDPRL
                     EILKSMISKPKGE"
     gene            3137..>3659
                     /locus_tag="VN363_04125"
     CDS             3137..>3659
                     /locus_tag="VN363_04125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558842.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="non-canonical purine NTP pyrophosphatase"
                     /protein_id="HWQ83727.1"
                     /translation="MNVLLIATNNPGKVIELQALLGILDLKLVTPAEIGIDLDVEETG
                     STYAENAALKAVAFGQASGLLTLADDSGLEVDSLDGEPGLHSRRYSPVPGATDADRRQ
                     YLLAHLASKPRPWTARFRCSIAILESEQAHSSTPHIYYAEGTCPGEIIPEERGENGFG
                     YDPIFLLSELKKTM"
BASE COUNT          879 a         1019 c          976 g          785 t
ORIGIN      
        1 gaatatctgg agagccttta gttggacgca attgaagtgc aggcacgcct tgaggcctcc
       61 ggaaaactga tcccgatcga atttagctgg ctgggacgca cttattttgt cagcagcata
      121 ggccggcgct ggaaagacag ccagggacag catatcctgg tccaggcaaa cggcgagcaa
      181 gtctacgagc tgctcttcca ggctggtgct gagcagtggt ggctggttaa aaagccggtt
      241 tttccatcgg tggcttgatg ctaactgcat aataaatcgc cccaccagaa ccgggtgttc
      301 tggcggggcg attttgatta atttgacagg caggcttact gacccagacg ggcgcgttcc
      361 tcggcgacca cactttcttc cagcttgcgg aataatgggg ctggctggac caatttctgc
      421 ccggcaggca gctggctcgg ctcccagcta ccgcgggcgg tatctgcccg gtagcggagc
      481 acctggtgag tccccagttt gtcctcgaca acctcggtat actgctcgcc aaacaacggc
      541 tgctgatatc ccaggtaagt gtttagttgt tcactcgtga agggcaggaa aggcgccagg
      601 agagttttga gagaatcgat gcagcgcaag gcggtataca ccgtcgtcgc cgcagcggct
      661 ctgtcagtct tgatctcgaa ccagggggca gtggtatcca gatacttgtt cacttcggaa
      721 gccagccgga ttgcttcgct cagcgcagcc cgcaagcgga cagccttcag atgctcgccg
      781 cattccagaa agcccgcctc gacaaccgcc aggatagctt catctcctga acgtaaaaca
      841 cccggctccg gaatgacgcc ttcccagttt cgataagcaa acgaaagcac ccggttggcc
      901 aggtttcccc aggtcgccac cagctcatca ttattccggt gtacaaagcc atcccaatcc
      961 cagtcggtat ctttgctttc aggcatattg accgtcagat aataacgcag tggatccgga
     1021 tcatagcgct ccaggaaatc cagcccccag acagcccaat tgcgactgcc agagattttc
     1081 ttgccttcca gattcataaa ttcattcgcc gggatatcaa acggcaaggt gagcggggca
     1141 tcggggcgct cttcgaagat ttgagaaaac gcctcgccgg cgccgatcag ctgcccgggc
     1201 cagatgactg catggaaggg aatattgtcc ttgccaatga agtagtaaga gcgcatctcg
     1261 gggttggtcc accagtcgtg ccagccatct ttggaaccat ccaggtgcga ccattcgatg
     1321 gctgcagaaa gatagccaat gaccgcctcg aaccagacat acaggcgctt accttcccag
     1381 ccctccactg gcaccggaat accccaatcc aggtcgcgag tgatcgctcg cccgtgcagt
     1441 gcattcgcct cgatctgtcc gagggattgg cgcagcacat tgggacgtaa atagctctcc
     1501 cgctggcgga gaaattccac aatctcaggc tggagcttgc ccagatcgag gtagaaatgc
     1561 tccgtctggc gcagatcggg cgtggagcca tcaatcttcg accggggatt gatgagctga
     1621 gtcgcatcga gcagctcacc gcagttatcg cactgatctc cccgggcatt ggcataacca
     1681 cagatataac acgttccttc tatatagcga tcgggcaaaa accggttctg gttcggggca
     1741 aaccactgct gctgactttc ggtgtagaga taaccatttt cccgcagtgc caggaagatc
     1801 gcctgggaaa cccgaaagtg attctcggtg tgcgtgctgg taaaaagatc aaatgagatt
     1861 cccagtttct ggtagagatc cagaaaacct gcatggaagc gctcgtaaac tgcaaccgga
     1921 gtgctgcttt ctgcatcagc ccggaccgtc accggagtgc catgcgaatc cgtgccagat
     1981 accatgatca cctggttgcc tgccaggcgc tggtaacgag caaaaatatc cgccggcagg
     2041 gaagaaccgg tcagattgcc gacatggatc aatgaattcg cataaggcca ggcaacagca
     2101 actaaaatat gctcggacat atgaacctcc ggattacctg gtttcctgga gcgctcctca
     2161 gcgcttacag gaggtcgtaa aaggctagca aagaaaaggg ctgcccatcc ggacagccct
     2221 ggctctacaa gaatcggtgt ctcagcaccc ggtctggttt cggcaggctg ccctgggagt
     2281 ggcgcgcagg cacatcgaca ttgccatgaa gaaaaacgat ctcaccactg acagaccttc
     2341 tcccaccatt tagttcacct ttcaacaatc aatccagacg gacagggatt cgagattaca
     2401 ctgagtctaa aaccctgtga atattttgtc aaggcaatca aaatcccgaa cgtttcgcct
     2461 ggtttcactc gcctttgggc ttgcttatca tggatttcaa gatttccagg cgaggatcga
     2521 tcacttcagc gctgtgctcg cactggacct cattgcgatt atcaccacac accgggcaga
     2581 gtcccttgca atccgggcgg cacaggggat tgatcggcag tgagagtaaa agctcttccc
     2641 ggatagaagg ctccaaatcc agctggccat tctctggaag caccaggcca gactcggcag
     2701 ccagcttagg cgtaaaggca tataaatcag taaaatttgc actcacttgc tgactgaaat
     2761 ccaccaggca gcgcccacat tgggcagtcg tctcgccctg cacctgcacc tgaacgacca
     2821 ggccttgctg ggcgcgtgta atccgcacaa cgccggaaaa ctgctcaata tccaggtctg
     2881 gcggcagatg aataaacggc aaatccaggg ggaattcccg gatatagccg atcgtttcgt
     2941 ggatgataaa gcctacatta aggcggaggt gattggttgc gttcaaggct gtttgtccaa
     3001 aagggaatta ataaaatgaa gttctctcag cagggcgatt attatatcat aggctcattt
     3061 tcgactgcga atctttaggt aaaataaggt ccggctgtct accccagcca tctgaaaact
     3121 cgaaagcgag acatctatga acgtactatt gatcgcgacc aataatcccg gcaaggtcat
     3181 cgagctgcag gcgctcctgg gaattctcga cctgaaactg gtcaccccgg ctgagatcgg
     3241 gatcgatctg gatgttgagg agaccggatc gacctatgct gaaaatgccg ccctgaaggc
     3301 ggtagcattt gggcaggcgt ccgggctgct caccctggcg gatgattccg ggctggaggt
     3361 agactccctg gatggagaac ccggattgca ttcacggcgc tattctcccg tgcccggcgc
     3421 aacagatgct gaccggcgcc agtacctatt ggctcattta gccagcaaac cccggccctg
     3481 gacagcccgc tttcgctgta gtattgctat cctggaatcc gagcaagccc attcatccac
     3541 acctcatatt tactacgccg agggtacctg ccccggggaa atcatccccg aagagcgcgg
     3601 cgagaacggc tttggttatg acccgatctt cttgctatcg gaattgaaaa agacaatgg
//
LOCUS       DATMXK010000221         3107 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_114229, whole genome shotgun sequence.
ACCESSION   DATMXK010000221 DATMXK010000000
VERSION     DATMXK010000221.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3107)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3107)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3107
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114229"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1430
                     /locus_tag="VN363_04130"
     CDS             <1..1430
                     /locus_tag="VN363_04130"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWQ83728.1"
                     /translation="VTIQLGNFQSDASGHFQSIVNLPNRQPTPETQYIRSITRANVGG
                     PELSKNGKDTWDKIIETVFLALLATTFGTLFAIPVSFLAAKNLMKDVVSPLASVTLSL
                     IAWPIGIGAGWLAARGAHWINDQFGQTTLLLIAGLIIMPLLTFFAMRIGLPPTDTRIP
                     TTRQRLGRYGALAVSAVAGIVAALLISQVVTYLGEFVTRVPALEFLGSFFVTVSDMVA
                     TFLALVVALATGALFSSWAERLGHWMTDTLSISVLKTANLILSGLAGALMVLIVAYII
                     EWLYDINNPLWVFVIPAILGALGGLLVAARLPHKGSLPVGMAIYTSVRAVMNVLRSIE
                     AFIWAIIFVVLVSTGPFAGTLALTLHTVAALGKLYSEQVESIMPGPLEAIKATGATRL
                     QTIIYGVIPQIIPPYISFTMYRWDINVRMSTIIGFAGGGGIGFLLQQNINLLNYRAAS
                     TQMIAITVVVWAMDYLSSVLRERYV"
     gene            1538..2200
                     /locus_tag="VN363_04135"
     CDS             1538..2200
                     /locus_tag="VN363_04135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559514.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ElyC/SanA/YdcF family protein"
                     /protein_id="HWQ83729.1"
                     /translation="MWHKIMRWIGKITVTLLLLTGLALLVPRLVTAIHAGPRLLSLQE
                     APARTVAIVFGAGLQRDGTPTAVLRDRVATAAELYFSGKVKKILMSGDNRFVEYNEPG
                     AMAAYAIALGVPEADIVLDYAGRRTYDTCYRARQIFGIQEALLVTQRFHLPRALYLCS
                     QLGIQALGVDADRQTYRRRSLLIWHLRETPATLVALWETHISHPLPVLGDPEPIFPLE
                     AQ"
     gene            2200..2760
                     /locus_tag="VN363_04140"
     CDS             2200..2760
                     /locus_tag="VN363_04140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559513.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWQ83730.1"
                     /translation="MEREAALALVREFVKNESLVRHMLAVEAAMRYYARLEGEDEELW
                     GNAGLLHDFDWEIHPSMAEHPEAGAPILRQRGVDETLIRAVLSHADHSGVPRQSRMEK
                     ALAACDEITGLVTAVALVRPSHSLYDLEAASVKKKWKDKAFAAGANRDEMVQSAQEYG
                     VELWEHVGNVIQAMRGIAPELGLDGQ"
     gene            complement(2873..>3107)
                     /locus_tag="VN363_04145"
     CDS             complement(2873..>3107)
                     /locus_tag="VN363_04145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83731.1"
                     /translation="PNTLYPEAASGSEVSWPQAYGMLMNGEVSQVMQPNAEKFTLLLK
                     DGRSLVAIQPAPGDVLAAIESCGATCTTIEVSE"
BASE COUNT          571 a          963 c          953 g          620 t
ORIGIN      
        1 gggtaaccat ccagctggga aatttccaga gcgatgccag cgggcatttc cagagcatag
       61 ttaatttacc caaccgccaa cccacccccg agacccagta tattcgcagt atcacccgcg
      121 caaatgtcgg gggaccggag ctatccaaga atgggaaaga tacctgggac aagatcattg
      181 aaaccgtctt cctggcgctg ttggcgacca ccttcggcac tttgtttgcg atcccggtaa
      241 gcttcctggc cgcgaaaaac ctgatgaagg atgtggtcag cccgctcgcc agtgtgaccc
      301 tctcgctgat cgcctggccg atcggtatcg gggcgggctg gctcgcggcg cgaggagcgc
      361 attggatcaa cgatcaattc ggacaaacga ccctgctgtt gatcgccgga ctgatcatca
      421 tgccgctgct gacatttttc gccatgcgga tcgggctgcc gccgactgac acccgcatac
      481 caacgacccg gcagaggctc gggcgctacg gcgctctggc cgtctcggcg gttgccggga
      541 tcgtagcagc cctgttgatc tcgcaggtgg tcacctatct cggggaattc gtgacccggg
      601 tgcctgcgct tgaattcctg ggctctttct tcgtcaccgt aagcgatatg gtagctacct
      661 tcctggcatt ggtcgtggcc ctggcgaccg gtgccctgtt cagcagctgg gcagagcgcc
      721 tgggccactg gatgaccgat accctctcga tttcagtctt gaaaacagcc aatctgatcc
      781 tgtctgggtt ggcaggcgcg ctgatggtgc tcatcgttgc ttacatcatc gagtggttgt
      841 atgacatcaa caatcccctg tgggtctttg taattccagc catcctgggc gcgctgggcg
      901 ggctgctggt ggccgcccgc ctgccgcata agggctccct gccggtaggc atggcgatct
      961 atacctcagt gcgcgccgtc atgaatgtcc tgcgctcgat cgaggcgttc atctgggcga
     1021 tcatcttcgt tgtcctggtc agcacgggcc cattcgccgg caccctggcg ctgaccctgc
     1081 acacggtcgc cgccctgggc aagctctact ccgaacaggt ggagagtatc atgcccggtc
     1141 cgctggaagc catcaaagcc accggtgcga cccgcctgca gacgatcatt tacggggtca
     1201 tcccgcagat catcccgccg tatatctcct tcaccatgta ccgctgggat atcaatgtgc
     1261 gcatgtccac catcatcggc tttgccggcg gcggcgggat cggcttcctg ctgcagcaga
     1321 atatcaactt gctcaactac cgcgccgcca gcacccagat gatcgccatc accgtggtag
     1381 tctgggcgat ggattacctc agctcggtgc tgcgcgagcg ctacgtctaa ataaccaaag
     1441 actggaaccc tcagctcctg ccgggacagt ccaccggcag gagcattcat tcccctggca
     1501 gggcgaagta cgccgccggc gtggtaaaat ttggccgatg tggcataaaa tcatgcgctg
     1561 gatcgggaaa attacggtca ctttactcct gctgaccggg ctggctttac tcgtcccgcg
     1621 cctggtcaca gccatccatg ccgggccgcg cctgctcagc ctccaggaag cgccggcacg
     1681 aaccgtggcg atcgttttcg gggccgggct gcagcgggat ggcaccccca cggcggtgct
     1741 gcgcgaccgg gttgccaccg cagccgagct ctatttttcc ggaaaagtca agaagatcct
     1801 gatgagcggt gataaccgct ttgtcgaata caacgagccc ggggcgatgg ccgcctatgc
     1861 catcgcgctg ggcgtgccgg aggcggatat cgtcctggat tatgccgggc ggcgcaccta
     1921 cgatacctgc taccgggcgc ggcagatctt cggaatccag gaggcgctgc tggtgaccca
     1981 gcgtttccac ctgccgcggg ccctgtacct gtgcagccag ctgggtattc aggccctggg
     2041 cgtcgatgcc gaccggcaaa cataccggcg ccggtcactg ctgatctggc acctgcgcga
     2101 gaccccggca accctggtcg ccctgtggga gacccatatc tctcacccgc tgccggtgct
     2161 gggcgatccc gaacccatct tccccctgga ggctcaataa tggaacgtga agcagccctc
     2221 gccctggtgc gagaattcgt taagaacgaa agcctggtgc gccatatgct ggccgtagaa
     2281 gccgccatgc gctactatgc ccgcctggag ggagaggatg aagagctgtg gggaaatgcc
     2341 gggctgctgc atgatttcga ttgggagatc caccccagca tggccgagca cccggaggcc
     2401 ggcgcgccga tcctgcgcca acgcggcgtg gacgagactc tgatccgggc ggtgctgagc
     2461 catgccgacc acagcggtgt gccgcgccag agccgcatgg aaaaagccct ggcagcctgt
     2521 gacgagatca ccgggctggt gacggccgtg gcgctggtgc gcccttccca ctcactctat
     2581 gaccttgaag ctgcctcggt gaaaaagaaa tggaaagaca aagcctttgc cgctggcgcc
     2641 aaccgggacg agatggtcca gtcggctcag gaatacgggg tcgagctgtg ggagcatgtg
     2701 ggcaatgtca tccaggcgat gcgcggcatc gctcccgagc tggggctgga cggccaataa
     2761 aacagaagcg ggggtgctgc tggtagcagc acccccgtcc gatttgtttc aggcttgaat
     2821 atcttctgga ttgagcgatc ttcccggaag ggaggtacct cgcttcgcgc ggttattcgc
     2881 tcacttcgat ggtggtgcag gtagcaccgc aggattcaat cgccgccagg acatccccgg
     2941 gggcgggctg gatcgcgacc agggagcgcc catctttgag cagcagggta aatttctcgg
     3001 cgttcggctg catgacctgg ctcacttcgc cgttcatcag catgccgtat gcctgcggcc
     3061 agctcacttc ggagccgctg gcagcttcgg ggtagagtgt attgggc
//
LOCUS       DATMXK010000222         2115 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_109956, whole genome shotgun sequence.
ACCESSION   DATMXK010000222 DATMXK010000000
VERSION     DATMXK010000222.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2115)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2115)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2115
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109956"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..634
                     /locus_tag="VN363_04150"
     CDS             <1..634
                     /locus_tag="VN363_04150"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWQ83732.1"
                     /translation="TLYSMWVKTEAGYKFVDQSAEDSPFSFVLGRGDAVFPGWDEGAT
                     GMKVGGKRLLVIPPELAMGEQENGVIPANSILVMEIELTEVKEPQVATPVEETDYTTT
                     ESGLKYYDLKTGTGNSPAPGQTVVVHYTGWLLDGTQFDSSIERGEPISFVFGEGNVIP
                     GWDEGLATMKVGGKRQLVIPAALGYGDQGAGTLIPPGATLLFEVELLEVQ"
     gene            638..1126
                     /locus_tag="VN363_04155"
     CDS             638..1126
                     /locus_tag="VN363_04155"
                     /inference="COORDINATES: protein motif:HMM:NF025793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine/deoxycytidylate deaminase family
                     protein"
                     /protein_id="HWQ83733.1"
                     /translation="MRPDWDSYFMKIADAVSERSTCDRAFVGCVLVLDKRILTTGFNG
                     SPAGQQHCDEIGHLLVDGHCVRTIHAETNAIIQAALHGVSTRGSTCYVSHFPCINCAK
                     ALINAGISRLVYQVGYRMDENAIEFLRTAEIEIVHLNHDPLQLSGADSNLARLADEGG
                     AR"
     gene            complement(1282..>2115)
                     /locus_tag="VN363_04160"
     CDS             complement(1282..>2115)
                     /locus_tag="VN363_04160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83734.1"
                     /translation="PTIPAAGEPTPAAGDPLPASPSGAPSFDLDELGRLTPDAVLLEL
                     AYEPTFFRPEASYEFGRPPVFALLADGRVIYTLEGESYEDEQVMVAQLTAEETVALVQ
                     QVLDLGFERLESYTDFCFTPAGAEQTCVADAAYTLLRLRTAADGMDEVRIYADFANDL
                     PAFESIRDLLANYTHAAAQPYAPQEAALFLSEEPGDAPGSVLAWPLDPALLAFPQNDF
                     NLWAIKLEGQQLSDYIAATGRNTGDAFFEHEGKVYRAFLSPWLPAAGYGEALIAAFPL
                     P"
BASE COUNT          449 a          580 c          714 g          372 t
ORIGIN      
        1 caccctgtac agcatgtggg tgaaaacgga ggctggctat aaatttgtcg accagtcagc
       61 cgaagattcg ccgttcagct ttgtgctggg gcgcggcgat gcggtcttcc ccggctggga
      121 tgaaggggcg acggggatga aggtgggcgg caagcgcctg ctggtgatcc cgccggagct
      181 ggcgatggga gaacaggaaa acggggtgat cccggccaat tctatactgg tgatggaaat
      241 cgagctgacc gaggtaaaag aaccccaggt tgccactccc gtggaagaga ccgattacac
      301 taccacagag agcgggctga aatattacga ccttaagacc ggtaccggaa acagcccggc
      361 acccgggcag acggtggtcg tccactacac cggctggctg ctcgatggaa cccagttcga
      421 cagctcgata gagcgtgggg agccgatcag ctttgtgttt ggagagggca atgtcattcc
      481 cggatgggat gagggcctgg cgacgatgaa agtaggcggg aagcgccagc tggtgatccc
      541 ggcggcgctg ggctatggcg accagggagc aggcactctg attccgcccg gcgcgacact
      601 gctcttcgaa gttgagctgt tagaggtcca gtaaaccatg cgcccggatt gggattcgta
      661 tttcatgaaa attgccgatg cggtttccga acgcagcacc tgcgaccgcg cctttgtggg
      721 ctgtgtgctg gttctggata aacgcatcct gaccaccgga ttcaacggct cgccggcggg
      781 gcagcagcac tgcgatgaaa tcgggcattt gctggtagat gggcactgtg tgcgcaccat
      841 tcacgccgaa accaatgcca tcatccaggc ggcactccac ggcgtttcga cgcgcggcag
      901 cacctgttac gtgagccatt ttccatgcat caactgcgcc aaagcgctga tcaatgccgg
      961 catttcccgg ctggtgtatc aggtcggtta ccggatggat gaaaacgcga tcgaatttct
     1021 gcgcaccgcc gagattgaaa tcgtgcacct gaatcacgat ccactgcagc tgagcggggc
     1081 agacagcaat ctcgcccggc tcgcagatga gggtggggcg aggtaagcgt gccaacaggg
     1141 ccagaggtag ttttgcctgc ctgaaagtga agaaacaaat aaccggcgtt cagtcgataa
     1201 cgattgagcg ccggttttga ttccggaggt gtgaaccaga ttgaggggcg tccgccaaag
     1261 gcggccgcct gcggcagtcg tttacggcag ggggaaggcg gcaatcaaag cctcgccgta
     1321 gccggcagcc ggcagccagg gagagaggaa agcccggtag actttaccct catgctcgaa
     1381 gaaggcatcg ccggtattgc gcccggtggc agcgatgtag tcgctgagct gctggccttc
     1441 cagcttgatc gcccacaggt tgaaatcatt ctgcggaaac gccagcaggg cgggatcgag
     1501 cggccaggcc agcacagagc caggggcatc acccggttcc tccgacagga acagggcggc
     1561 ttcctggggg gcgtaaggct gcgctgcagc atgggtgtaa ttggccagca ggtcacggat
     1621 gctttcgaaa gcaggcagat cattggcgaa gtcggcgtag atgcggactt cgtccatgcc
     1681 atcggcggca gtgcgcaggc gcaggagggt gtaagcggca tcggccacgc aggtctgctc
     1741 agcgccagcc ggcgtgaagc agaaatcggt gtaactttcc aggcgctcaa aacccaggtc
     1801 gagaacctgc tgcaccagag cgaccgtttc ttcggcggtc agctgggcga ccatcacctg
     1861 ctcatcttcg taggattcgc cttccagggt gtagatcacc cggccatcgg ccaggagggc
     1921 gaaaaccggc gggcggccaa actcataaga ggcctcgggg cggaagaagg taggctcgta
     1981 ggccagctca agcaggacgg catccggcgt caggcggccc agctcatcca ggtcgaagga
     2041 gggcgcgccg gatgggcttg ccggcaaagg atccccggct gccggggtgg gctcaccggc
     2101 tgccgggatg gtagg
//
LOCUS       DATMXK010000223         2431 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_139001, whole genome shotgun sequence.
ACCESSION   DATMXK010000223 DATMXK010000000
VERSION     DATMXK010000223.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2431)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2431)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2431
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139001"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..443
                     /locus_tag="VN363_04165"
     CDS             <1..443
                     /locus_tag="VN363_04165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83735.1"
                     /translation="QPQIASVVPAEVHRLTSLAMTKYRDVIASHALWRSNPAPAHPLS
                     CQPQIASVVPAEVHRLTSLAMTKYRDVIASHALWRSNPAPAYPISCQPQIASVVPAEV
                     HRLTSLAMTKIAMSLRAKRSNLSPTFSQSCQLQIVSVAQTAPSQ"
     gene            complement(538..>2431)
                     /locus_tag="VN363_04170"
     CDS             complement(538..>2431)
                     /locus_tag="VN363_04170"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83736.1"
                     /translation="AIRAGTGNGWEFAAEALQASLGLWQGVECFVDHEQNPGSLRGGR
                     SLRDLGGVFENPAWDAAEQAVRLELRTLGPSGGLVEALGRAMLAESHNDIRQNAALPK
                     VGFSADILFTAQGKRVERIVRVFSVDLVFNPARGGAFVRALNSMQMDGVQGGRPMAET
                     MNEMEGAVQEGSPAVVEAANSAGLRAQLSACLLEAGLGAARLPAPLAEHVRQQFSGRE
                     VTVEALNRAIGEARGLASALGGGLTVSGPGGYPGIRGMYDTRDQLQAAVDDLLEAPRS
                     PGMQNLKAARLSGIKELYMLLTGDRDLYGGYYAERAQLATTATFPGLVKNAMNKIVAE
                     QWSLLGAAGYDWWKKVVQVEHFETLNQVTGILVGTVGSLPSVAEGGEYTELSMGDSPE
                     TADFAKYGGYIPLTLELIDRDNTRKLRQYPRELAKAGMRKLSQLVAAVFSANSGVGPT
                     MADTGALFNATAVTTAGGHANLRTGALSSAEWETVSAAVYNQPLLIRNAAGVYGTGPK
                     QAVDPKYLLVPRELRLTAMKVLYPTWENTANIHSENQQRGELGDVVVVPEWTDAADWA
                     AVVDPRLAPGIVVGERFGLMPEVFIAGDELSPAVFMNDEHRMKVRHFLAVLVQDFRPL
                     HKNNVA"
BASE COUNT          414 a          919 c          633 g          465 t
ORIGIN      
        1 gccagcccca gattgcttcg gtcgttccgg cggaagtaca ccgcctcacc tccctcgcaa
       61 tgacaaaata tcgcgatgtc attgcgagcc acgctctctg gcgaagcaat ccagcgccag
      121 cccacccact atcctgccag ccccagattg cttcggtcgt tccggcggaa gtacaccgcc
      181 tcacctccct cgcaatgaca aaatatcgcg atgtcattgc gagccacgct ctttggcgaa
      241 gcaatccagc gccagcctac ccgatatcct gccagcccca gattgcttcg gtcgttccgg
      301 cggaagtaca ccgcctcacc tccctcgcaa tgacaaaaat cgcaatgtca ttgcgagcga
      361 agcgaagcaa tctatcgcca accttctcac aatcatgcca actccagatt gtttcagtcg
      421 cacagaccgc tccttcgcaa tgacatcatt cacagagcat tattcttttc ctgcgccgcg
      481 ctgcgaaatc tgcggacctt cagtctaatt cgtgaaattc gtggtcaaaa ttcgttgtca
      541 ggccacgtta ttcttatgca gcgggcggaa atcctgcacc agcaccgcca ggaaatggcg
      601 taccttcatg cggtgctcat cgttcatgaa caccgccggc gaaagctcgt ccccggcgat
      661 gaacacctcc ggcatcagcc cgaagcgctc acccaccacg atcccgggcg ccaggcgtgg
      721 gtccaccacc gctgcccaat ccgcggcgtc cgtccattcc ggcaccacca ccacatcgcc
      781 cagctcaccc cgctgctggt tctccgaatg gatattggcg gtattttccc aggtcgggta
      841 aagcaccttc atcgccgtca ggcgcagttc gcgcggcacc agcaggtatt tcgggtccac
      901 agcctgcttc ggccccgtcc catatacccc ggccgcgttg cggatcagga gcggctggtt
      961 atacactgcc gcgctcaccg tctcccattc tgcgctcgag agcgctcccg tgcgcaggtt
     1021 ggcgtgcccg ccggcggtcg tcactgccgt ggcattgaac agcgcccccg tatctgccat
     1081 ggtcgggcca accccgctgt tggcgctgaa cacggccgcc acaagctgcg agagcttgcg
     1141 catcccggct tttgccagct cgcgcgggta ctggcgcagc ttgcgggtat tgtcccggtc
     1201 gatcagctcc agggtcagcg ggatataccc gccgtacttg gcaaaatccg ccgtttccgg
     1261 gctgtcgccc atgctcagct cggtgtattc gccgccctcc gccacactgg gcaggcttcc
     1321 caccgtcccc accaggatgc ccgtgacctg gttgagggtc tcgaaatgct ccacctggac
     1381 caccttcttc caccagtcgt agcccgccgc ccccagcagg ctccactgct cagcaacgat
     1441 cttgttcatg gcgttcttca ccagccccgg gaaggtggcg gtggtcgcca gctgcgcccg
     1501 ctcggcgtaa tagccgccgt acaggtcgcg gtcgccagtc agcagcatgt acagctcctt
     1561 gatcccgctc agccgcgccg ctttcaggtt ctgcattccc ggactgcgcg gcgcttccag
     1621 caggtcatcc accgctgcct ggagctggtc gcgcgtgtca tacatcccac ggattccggg
     1681 atagccgcca gggccgctta cggtcaggcc accccccagc gccgaggcca gcccgcgcgc
     1741 ctcgccgatt gcccggttga gcgcttctac cgtcacttcg cgcccgctga actgctggcg
     1801 cacgtgttct gccaggggag ccggcagccg cgccgctccc aggcccgctt ccagcaggca
     1861 tgcactcagc tgcgcccgca ggccagcgct gttcgccgcc tccaccaccg ccgggcttcc
     1921 ctcctggact gctccttcca tttcgttcat tgtctctgcc atcggtctgc ctccttgaac
     1981 tccatccatc tgcattgaat taagtgcccg cacgaacgcc ccgccgcgcg ccgggttgaa
     2041 caccaggtcc actgaaaaga cccgcacgat cctttccacc cgcttgccct gggctgtgaa
     2101 aaggatatcc gccgaaaagc ccactttggg caacgccgca ttttggcgta tgtcattgtg
     2161 ggattccgcc agcatcgccc ggcccagtgc ctccaccagg ccaccgctcg ggcccagggt
     2221 gcgcagctcc agccgcaccg cctgctctgc cgcgtcccag gccggattct cgaacacccc
     2281 gcccagatcg cgcagcgagc gcccgccgcg caggctgccc ggattttgct cgtgatccac
     2341 gaagcactcc accccctgcc acagccccag cgaagcctgc agcgcctccg cggcaaactc
     2401 ccagccgttc ccggttcccg cccggatcgc a
//
LOCUS       DATMXK010000224         6121 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_112066, whole genome shotgun sequence.
ACCESSION   DATMXK010000224 DATMXK010000000
VERSION     DATMXK010000224.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6121)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6121)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6121
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112066"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..470
                     /locus_tag="VN363_04175"
     CDS             <1..470
                     /locus_tag="VN363_04175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83737.1"
                     /translation="RLGQLICWTLALTGCDLQVLARRPRQQELLLQRGIRVITADQLQ
                     SRHYDIVVEATGTPDGFYLARQALRARGTLVLKSTYKGDLQVNLSAVVVDEITLVGSR
                     CGPFKPAIGLLESGKIDPRDLIEACYPLEAAEEAFAEAAKPGVLKVLIKPDNP"
     gene            complement(467..1687)
                     /locus_tag="VN363_04180"
     CDS             complement(467..1687)
                     /locus_tag="VN363_04180"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HWQ83738.1"
                     /translation="MTDLEIERRLERYQRVIAISNELALTLDLDRLLNQVVHAAAELC
                     DAQAASILLYDEVKSVLNFSAASNLSEPMMRGLVVPVASSIAGWIVTHKEPVIIDNAD
                     EDPRHFGGVALATNITTTSMLGVPMLAKGKVIGALEAINKLSGKFTEEDQELLMVLGV
                     QTAIAIENARLFHQSDLISELVHELRTPLTSLSTAARLLLRPNLSPEMRTQVTEIITA
                     ETDRLTEMTTAFLDLARLESGRATFDIEPFDIQLLISECVSQMRAKAEEDKNLCIELI
                     LPGNEQGLPDLVADRDKVKQVLLNLLSNAIKYNSPCGEITVRASQVRDNLEIRVQDTG
                     PGIPPESLPHMFEKFFRVPGIEKSIQGTGLGLYICKRIMDAHHGSIDIVSQPGKGSTF
                     ILTFPIGLTGTPDA"
     gene            complement(1680..2465)
                     /locus_tag="VN363_04185"
     CDS             complement(1680..2465)
                     /locus_tag="VN363_04185"
                     /EC_number="2.3.1.286"
                     /inference="COORDINATES: protein motif:HMM:NF001753.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0017136 - NAD-dependent histone
                     deacetylase activity [Evidence IEA];
                     GO_function: GO:0070403 - NAD+ binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent deacylase"
                     /protein_id="HWQ83739.1"
                     /translation="MAEMIQLSPATQTDIRDAADIIRGCRQGVVLTGAGLSTPSGIPD
                     FRSTNSGLWERFDPAEVASLSTFRYHPEKFFAWIRPLVAQMAVAQPNIAHQSLAFLET
                     KGYLKTTITQNIDGLHQKAGAKNVLEVHGSLETLTCVKCFSKLGSADYIAPLIEAGEI
                     PHCPTCGGILKPNLILFGEQLPSRTWLTAQEACRKADLMIIAGSSLEVLPVAGLPMRA
                     LENGAHLIIINQTQTYLDLRADVVFREDVLDILPRIVKEVACD"
     gene            complement(2466..3623)
                     /locus_tag="VN363_04190"
     CDS             complement(2466..3623)
                     /locus_tag="VN363_04190"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ83740.1"
                     /translation="MAEKILIVDDDIDTLRLVGLMLQRQGYEISAASNGIQALAMVQA
                     EHPDLILLDLMMPDIDGVEVTRRLRSADDTKDVPIIMFTAKTQVEDKIEGFEAGADDY
                     LTKPTQPRELFVHVKAVLARTNKTKKTGPIGMPKGDRGYVVGVLAARGGLGVSTLVLN
                     LGVALAQRYKKDVILGEFRPGQGIISLELGQTRADGLTHLLQLDPTAIDRRALDSELV
                     SHSTGVRLLLASPQPRDAKYMNRPEVYEAIARQLAYMTRYLLLDLGSSLTPMVEQVVG
                     LCNEIMVIVEPVPQTVLQTKALIEDLIDKGVPSDNVTTILVNRVRSGMQLSWSQVQDQ
                     LGRPVSMIFTPAPDLAYQASMKNIPMVLQQADSLTAQQFVKLAEKVAMKTN"
     gene            3720..5165
                     /gene="tilS"
                     /locus_tag="VN363_04195"
     CDS             3720..5165
                     /gene="tilS"
                     /locus_tag="VN363_04195"
                     /EC_number="6.3.4.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR02432.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016879 - ligase activity, forming
                     carbon-nitrogen bonds [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA lysidine(34) synthetase TilS"
                     /protein_id="HWQ83741.1"
                     /translation="MFNSLDNILQQECKLDRNLPILVGVSGGPDSICLLHCLIQAGYH
                     TVVAHFNHQLRPEADIEAGMVLHLAEDWKIPAIVKEADVRSHAEQNHLSIEEAARELR
                     YQFLFSTAGEQKAQAVAVGHNADDQVETILMHLLRGAGPAGLRGMRIRSLPNPWSKSI
                     PLVRPLLAIWRTDIEAYLQEYQLPANRDASNLDPVYYRNQLRHQVIPYLQTLHPQIKK
                     ALWQTAELVGTDYDFLLAEIQAKWDSDIIRNKSRGVIEINRQAFLDLAESLQRGLLRR
                     AAFELQPNLRDLDYSAIQRALHAIKLNGSELQVDLVSGIQLVVDSDRLWLVDQGSDIQ
                     TDEWPQISSATTILEELNLGTAREVQLENGWELHVEIVTCDEQVLSGALNNPDPYQAW
                     ADADRISEPLSLRTRMPGDQIQLLGMGGQHTNLSDLFINRKIPRRGRTKWPLIVSGNR
                     ILWVPGVQLAEPFRLTAVTQRAIHFSVIQRH"
     gene            complement(5241..>6121)
                     /locus_tag="VN363_04200"
     CDS             complement(5241..>6121)
                     /locus_tag="VN363_04200"
                     /inference="COORDINATES: protein motif:HMM:NF027968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="alpha-glucosidase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ83742.1"
                     /translation="PEDVRAYFGDGTDEFHMGFHFPLMPRIFKSLRTGERSSLEWILD
                     KTPALPGECQWCTFLRNHDELTLEMVTEEERQWMWEVYAPDPRMRLNLGIRRRLAPLL
                     DNQRSKIELANSLLFTMQGSPIIYYGDEIGMGDNIWLFDRNGVRTPMQWDSSPQAGFS
                     EAAPENFYSPVIESEQYSPKQINVFDLRGDPHSLYNTLRRMLAVRKQHPAFGRGTFAW
                     IGDNPHVAAYLRVSDTDQVLIVNNLSDEVQELVLEIDHWEKAPVNLLTGAFLEAGSEE
                     RLKLRLEPYAYLWLAV"
BASE COUNT         1511 a         1622 c         1521 g         1467 t
ORIGIN      
        1 gacggctggg acagttgatc tgttggactt tagcattgac cggctgcgat ctgcaggttc
       61 tcgcccggcg tcctcgccag caggaactgc tcctccagcg tgggattcgg gtgatcactg
      121 cagatcaatt gcagtcgaga cactacgata ttgttgtcga agcgacagga acgccagatg
      181 ggttttatct tgcgcggcag gctctgcggg cgcgcggcac actggtcttg aaaagcactt
      241 acaagggaga cttgcaggtg aatctttctg ctgtcgttgt ggatgagatc acactggtgg
      301 gctcacgctg tggacctttc aagccagcta ttgggctgct ggaaagtgga aaaattgatc
      361 cccgcgatct gattgaagcc tgctatcccc ttgaagctgc cgaagaagct ttcgccgagg
      421 ctgcaaaacc aggtgtttta aaagtattaa tcaaaccgga taatccttaa gcatccgggg
      481 tgccggttaa tccaatgggg aatgtcagga tgaatgtgct gcctttgccc ggctggctga
      541 cgatatcaat cgagccgtga tgtgcgtcca taatccgttt gcagatatac aggcctaacc
      601 cggtcccctg gatggacttt tcaattcccg ggacacggaa aaacttttca aacatatgcg
      661 gcaggctctc cggcgggata ccaggtccgg tatcctggac ccggatttct aaattatccc
      721 ttacctgact ggctctgaca gtaatctccc cgcaaggtga gttatatttg atggcatttg
      781 acagcaggtt cagtaaaacc tgcttgacct tatcccgatc ggcgaccaga tcaggaagcc
      841 cctgctcatt accgggcagg atcagctcga tacagaggtt tttgtcctct tcagcttttg
      901 cgcgcatctg gctcacgcac tcgctgatca gcagctgaat atcaaagggc tcgatatcaa
      961 acgtagcccg gcctgactcg agtcgcgcca ggtcgagaaa tgccgtcgtc atttcggtca
     1021 ggcggtctgt ttccgcagtg ataatttctg tgacctgggt ccgcatctca gggctcagat
     1081 taggccgcag aagcagtcgc gcggctgtgc tcaacgatgt gagaggggtg cgcaactcgt
     1141 ggaccagctc actgatcagg tcggactggt gaaaaaggcg ggcattctca attgcaatgg
     1201 ctgtctgtac ccccaacacc atcaacaatt cctgatcttc ttcggtaaac ttaccgctga
     1261 gtttattaat cgcttccagg gcgccaatga ccttcccctt cgctaacatc ggcaccccaa
     1321 gcatagatgt tgtggtgata tttgtggcca gcgctacgcc accgaaatga cgcggatcct
     1381 catcagcatt atcgatgatc accggttcct tatgggtaac gatccaaccg gcaattgagg
     1441 aagcaaccgg taccaccaga cctcgcatca taggttcgct cagattagac gccgcgctaa
     1501 aatttaacac actcttcact tcgtcataga gcagaatcga agcagcctgc gcgtcacata
     1561 actctgcagc agcgtggacg acctggttca ataaacgatc caaatccaag gtcaatgcca
     1621 gctcattgga gatggcgatg actcgctggt aacgctccaa cctgcgttca atctcaaggt
     1681 cagtcacagg ctacctcttt gacaattctg ggcaggatat ccagtacgtc ttcacgaaaa
     1741 acaacgtcgg cgcgcaggtc cagataggtt tgggtctggt tgatgatgat tagatgagcg
     1801 ccattttcca gggcgcgcat cggaagcccg gctaccggca gaacctccaa agaggatccg
     1861 gcaatgatca tcaagtccgc tttacgacag gcttcctggg ctgtcagcca ggtacgggag
     1921 ggtaattgtt ctccaaacag gatcaggttt ggcttcaaga ttcccccaca cgttgggcaa
     1981 tggggtatct caccggcctc aattaaaggc gcgatgtaat ctgcagagcc cagcttgcta
     2041 aaacacttca cacaggtcag agtttccagg gatccatgca cttccaacac atttttagcc
     2101 cccgctttct ggtgcagacc atcgatattc tgggtgatag tcgttttcag ataacccttt
     2161 gtctccagga atgccagcga ttggtgggca atgttcggct gagcaaccgc catctgagcg
     2221 acaagtggtc gaatccacgc gaagaatttt tcagggtggt agcgaaatgt gctgagtgaa
     2281 gcaacttccg cagggtcaaa ccgttcccac aggccactgt tcgtcgaacg aaaatcggga
     2341 attccagacg gcgttgagag acccgccccg gtcaatacaa caccctgccg gcagccacga
     2401 atgatatccg cagcgtcccg aatatcggtc tgtgtcgccg gtgataactg gatcatttca
     2461 gccattcaat tcgttttcat ggctactttt tcagcaagct taacgaattg ttgtgcagtc
     2521 aagctgtcgg cttgttgaag aaccatggga atattcttca ttgaagcctg ataggcaaga
     2581 tccggtgcag gtgtaaaaat catggatact ggacgcccta attgatcctg aacctgactc
     2641 caggaaagct gcatccccga acggacacgg ttcaccagga ttgtcgtaac attatcactg
     2701 ggaaccccct tatcgatcag atcttcaatc aacgctttcg tttgtaaaac tgtttgtggg
     2761 accggttcga caataaccat gatttcatta cacaagccaa ccacctgttc caccatagga
     2821 gtcagagatg aacccaaatc aagcaaaaga taacgcgtca tataagctag ctgtctggca
     2881 atagcctcgt atacctccgg tcgattcatg tattttgcat ctcgaggttg aggtgatgcc
     2941 agcagtaaac gcacgcctgt actgtgtgag accaattccg aatcgagcgc acgcctgtct
     3001 attgccgttg gatcgagctg cagcaagtgg gtcaggccat cggcgcgcgt ttgtcccagc
     3061 tccaaactga tgattccctg accaggccgg aattcgccca atataacatc ttttttataa
     3121 cgctgcgcca gggcaacccc aagattcaga accagcgttg atactcccag gcctccccgg
     3181 gcagccagga cgcctacaac atagccacga tcccctttcg gcattcctat gggtccagtt
     3241 ttcttggttt tattggttcg ggcaagaact gctttgacat ggacaaataa ttcacgtggt
     3301 tgggtcggct tggtcagata atcatccgcg ccagcctcaa atccctcgat cttgtcttca
     3361 acttgagtct ttgcggtaaa catgatgatc ggaacatctt tggtatcgtc agcactgcgt
     3421 aaacgacggg ttacctccac accatcgata tccggcatca tcagatccag caggatcaga
     3481 tcagggtgtt cagcctggac catagccagc gcctggatgc cattgctggc tgcgcttatt
     3541 tcgtagccct gccgttgcag catcaagccg accagacgta atgtatcgat atcatcgtca
     3601 acaatgagga ttttttcggc cactgctgct cctaaaaaat attacttagc gatctgggtc
     3661 tgattgttgg cttacttgaa gcgatgagca gaacaagatc agctataatt ttggctgtga
     3721 tgtttaactc tttagacaat attttacaac aagaatgcaa acttgaccga aacctgccaa
     3781 ttttggtggg agtatcggga ggtccagaca gcatttgctt gctgcattgt ctgattcagg
     3841 cagggtacca tacggtcgtg gctcatttta atcatcagct gcgtcctgaa gcagatattg
     3901 aagccgggat ggttctacat ctcgcagaag attggaagat tcctgccatt gttaaagaag
     3961 cagatgtacg cagccacgct gaacaaaatc acctgtcgat tgaagaagct gcccgcgaac
     4021 tacgctacca gtttttattt tcaaccgccg gagagcaaaa agcccaggcg gttgcagtcg
     4081 gtcataatgc agacgatcag gtagaaacca tattgatgca cctgctgcgc ggcgctggtc
     4141 cagccggctt acgcggcatg cgcatccgga gccttcctaa cccgtggagt aagtcgattc
     4201 ccctggtgcg ccccttatta gcaatctgga ggacagatat agaggcttat ctacaggaat
     4261 accagctgcc tgctaaccgg gatgcttcaa acctggatcc agtttattat cgtaatcaac
     4321 tgcgccatca agtgatccca tatttgcaaa cactccatcc ccagatcaag aaggctctct
     4381 ggcaaactgc cgagctggta ggcaccgatt acgattttct gctggccgaa atccaggcta
     4441 aatgggattc agatattatc cgtaataaat ccaggggggt aattgaaata aaccgccagg
     4501 catttttaga tctggcggaa agcctgcagc gtggtctgct cagacgtgca gcattcgaat
     4561 tgcagcccaa tttacgcgat ctggattaca gcgccattca acgggcttta catgccatca
     4621 agttgaatgg gagtgaatta caggtcgatt tggtatctgg tatccagctg gttgtagatt
     4681 ctgaccggct gtggttggtt gatcagggtt cagatatcca gacggacgaa tggccccaaa
     4741 ttagctctgc tactaccatt cttgaggagt taaatctggg cacagcaagg gaagttcagc
     4801 tggaaaatgg ctgggagctc cacgtagaaa ttgtcacctg tgacgaacag gttctgtccg
     4861 gcgcgctgaa taatcctgac ccatatcagg cctgggctga tgcggatcgg atctccgaac
     4921 cactgtcact gcggacacgt atgccaggag accagatcca actgctcgga atgggcggcc
     4981 agcataccaa tctctccgac ctgttcatca accggaagat accgcgccgg gggcggacaa
     5041 aatggccttt gatcgtctca ggtaacagga ttctgtgggt tccgggcgtt caattggctg
     5101 agcccttccg cctgactgca gttacccaac gcgcgatcca tttttcagtg atccaacgcc
     5161 attgacttcc cacccaaata aaacgaatgg ggcaggtcta aaattcgacc tgccccatca
     5221 gaatccaacc agtctctgct tcaaactgcc agccacaaat aggcatacgg ctccaaacgc
     5281 agcttcagcc tttcctccga accggcttcc aggaacgccc cggtaagcag attgactggg
     5341 gctttctccc aatgatcgat ctccaaaacc agctcctgga cctcatcaga aagattattc
     5401 acgatcaaaa cctggtctgt atccgaaacc cgcagatacg cagctacatg tggattatcg
     5461 cctatccagg cgaatgtgcc gcgcccgaat gcaggatgct gctttcgaac agcgagcatt
     5521 cgccgaagcg tattgtagag tgaatggggg tcaccccgga gatcaaatac attgatttgt
     5581 tttgggctat attgctcaga ctcgatcacg ggggaataga aattctctgg tgcagcctcg
     5641 gaaaaacctg cctgtgggct ggaatcccac tgcatcggtg tgcggacgcc attgcgatca
     5701 aacagccaga tattatcgcc catgccaatc tcatcaccat aatagatgat aggtgagcct
     5761 tgcatggtaa acagcagaga attcgctaat tcgatttttg atcgctgatt atccaacaag
     5821 ggtgccagcc ggcggcgaat acccaggtta aggcgcattc gcggatcagg agcataaact
     5881 tcccacatcc actgccgctc ttcttcagtc accatctcta aggtcagctc gtcatgatta
     5941 cgcaaaaaag tgcaccactg gcactctcca ggcagagcag gcgttttatc caggatccac
     6001 tccagggaac tgcgttcccc cgtcctgagg gatttaaaga tgcgcggcat caaggggaaa
     6061 tgaaaaccca tgtgaaactc gtccgtgccg tcgccaaaat atgcccgcac atcctcaggc
     6121 c
//
LOCUS       DATMXK010000225         3561 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_143773, whole genome shotgun sequence.
ACCESSION   DATMXK010000225 DATMXK010000000
VERSION     DATMXK010000225.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3561)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3561)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3561
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143773"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..290)
                     /locus_tag="VN363_04205"
     CDS             complement(<1..290)
                     /locus_tag="VN363_04205"
                     /inference="COORDINATES: protein motif:HMM:NF014672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DsrE family protein"
                     /protein_id="HWQ83743.1"
                     /translation="MPSELLGKKILIVVSGGPENPERCVGPFFFANSAARSKAEVSML
                     FVMGGSILLKQGIAETICAREGGRPVRVFLDDCLKSGVKLYVCAASLKLNDMV"
     gene            complement(405..1739)
                     /locus_tag="VN363_04210"
     CDS             complement(405..1739)
                     /locus_tag="VN363_04210"
                     /inference="COORDINATES: protein motif:HMM:NF014774.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(Fe-S)-binding protein"
                     /protein_id="HWQ83744.1"
                     /translation="MTDNRFSDERYQLTEEKSALALDVLRQRLNRQLEASLEVCVRCG
                     LCAESCHYYLANPKPEYVPAYRAEQLRKIYRQLFDPLGKAAPGWVGAAELDQEMVEKL
                     MLAAYGTCTMCGRCVINCPMGVDTRHIIRTARAMLTAMDMTPAGLKATVDVHLEVGNN
                     MGVSEEDYVETVQWMEEELQAELDDPGVKIPFNKKGARVVYTFNPREIKYFPYLIQAA
                     AKIFNAAGEDWTLSTESWDATNYGLFNGDDTAARQIAANLEGAVNHLGAETLVMAECG
                     HGYRSQRWEGENWAGHHYPFQVLSFVELQALYIKEKRFKLDPTVNSQRVTYHDPCNQA
                     RSGGIIEEPRYVLRHVATDFVEMTPHGAENFCCSGGGGMLSMTEFAKERIAAGKVKAD
                     QITATGAKIVVTSCHNCLDQLDEIKRHYKLPVKIQNLSELTAAAIVWEKKGQ"
     gene            complement(1750..2394)
                     /locus_tag="VN363_04215"
     CDS             complement(1750..2394)
                     /locus_tag="VN363_04215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83745.1"
                     /translation="MMSLLEITRIFAILGLVIGLAGILLRINEIMRRPFKQDLARPRG
                     DVRRGILYAFTLGMAPWEKESTRNHWVAYIRGILFHVGIFSAFGVLFASPWSEVLPDW
                     VKLIALAITGLGAVFGFAGIAMRLKGANERALSLPDDYFSVFLTSLFIALAFVVLLAP
                     GWMPAFYIVTGVMGVYIPLSKIRHCVYFFYSKFFFGSGFGRRGVIGQPKNEYSE"
     gene            complement(2408..>3561)
                     /locus_tag="VN363_04220"
     CDS             complement(2408..>3561)
                     /locus_tag="VN363_04220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83746.1"
                     /translation="AALTASEADAMRSRAWGLRAAAEAWLPVDPARAGQLLEAAEDTA
                     GGALGRARDYDLRTIALVWAELDPARAALVMEKVNDPAVRAWGYRELGDYASAADAAR
                     LVRDPVQKAGSLARIALAAGQGELFDEAHAALVAAQDQLTAGSFAFALSRLAVQSGDA
                     FILDDIAAEFPAARALAQLGLGQPAAAWETSQSVSDLYEQAHLQALIVPHLEDIEQAY
                     TIRVPALADAALRDIIQKTGTSSLIQRAASVYYKVQILTNLGDYATAWDRAVLADDPL
                     RESGALVPLACAWSQTDPAAAAGVLEQIDREADKAVVLRCLAGATGSQQDFERALGMA
                     LAARVRGDATAPVRASLTLARMFLDQGDSAKASAALQQAYDIALRISVK"
BASE COUNT          735 a         1100 c         1017 g          709 t
ORIGIN      
        1 accatatcat ttagcttcaa tgacgccgca cagacgtaca gttttacacc cgatttcagg
       61 caatcgtcca gaaaaacgcg caccggacgc ccgccttccc tggcacagat cgtttcggca
      121 atcccctgct tgagcaggat cgagccgccc atcacaaaca gcatgctgac ctcagccttg
      181 ctgcgggctg cgctgttggc aaagaaaaaa ggtccgacac agcgttctgg attctccggg
      241 ccgccgctga caactatcag gattttcttg ccgagcaatt cagatggcat gcttattatc
      301 cctaccggac cctacaggtt ttaatatttc ccgactccac cagatgacat ttgctcgtag
      361 gcatcctgtt ctgcccaggt tctgcggaaa cctgcagggt ctcattattg accctttttt
      421 tcccagacga tcgccgcagc ggtcagctcg ctcaggttct ggatcttgac cggcagcttg
      481 taatggcgct tgatctcatc cagctggtcg aggcagttat ggcaggaggt aacgacgatc
      541 ttagcgccgg tggcagtgat ctggtcggct ttgaccttgc cggcggcgat gcgttctttc
      601 gcaaattcgg tcatcgagag catccctccc ccaccgctgc agcaaaagtt ttccgccccg
      661 tggggagtca tctccacaaa atcggtggcg acatgccgca ggacgtagcg cggctcctca
      721 attatgccgc ccgagcgggc ctggttgcag ggatcgtggt aggtcacccg ctggctgtta
      781 acggtgggat cgagcttgaa gcgtttttct ttaatataca gcgcctgcag ctcgacgaag
      841 ctcagcacct gaaacggata gtgatgcccg gcccagtttt cgccttccca gcgctgcgag
      901 cggtaaccat gcccgcactc tgccatcacc agggtctcgg cgcctaaatg attgacggcg
      961 ccttccaggt tggcggcgat ctggcgggca gcggtgtcat cgccgttgaa caggccataa
     1021 ttggtggcgt cccagctctc ggtgctcaag gtccaatcct cgccagcggc attgaaaatt
     1081 ttcgccgcgg cctggatcag ataagggaag tatttgattt cgcgcgggtt gaaggtgtac
     1141 accacgcggg cgcctttttt attgaaggga atctttacgc cggggtcgtc cagctcagcc
     1201 tggagctctt cttccatcca ctgcacggtc tcgacataat cctcctccga gacgcccata
     1261 ttgttgccaa cttccaggtg gacgtcgaca gtggccttca ggccggcggg ggtcatatcc
     1321 atggcggtga gcatggctcg cgccgtgcgg ataatatggc gggtatccac acccatcggg
     1381 cagtttatta cgcaccgccc gcacatcgtg caggtcccgt atgccgccag catcagcttc
     1441 tcgaccattt cctggtccaa ctcagccgcg cctacccagc ccggggcagc cttgcccagc
     1501 ggatcaaaga gctggcggta gatcttgcgc agctgctcgg cgcgatacgc cgggacatat
     1561 tccggtttgg gattggcgag ataataatgg cacgattcgg cgcacagccc gcagcgcacg
     1621 cacacttcca gggaagcttc cagctggcgg ttcaggcgct ggcgcaggac atccagcgcc
     1681 agggcagatt tttcttccgt aagctgataa cgttcatcgc taaaccggtt gtcagtcaag
     1741 gttccctccc tactcgctgt attcgttctt cggctggcca attacacccc ggcggccaaa
     1801 acccgagccg aagaaaaatt tggaataaaa gaagtatacg cagtggcgaa ttttgctaag
     1861 cgggatatac acgcccatca cgccggtgac gatgtaaaag gcaggcatcc agccgggagc
     1921 cagcagaacc acaaatgcca gagcgataaa taacgacgtc agaaaaaccg aaaaataatc
     1981 atccggcagg ctgagggcac gctcgttcgc ccccttcaag cgcatggcaa tgcctgcaaa
     2041 accaaagaca gcccccaaac cggtaatcgc cagggcaatc aacttcaccc agtcgggcag
     2101 cacctccgac cagggactgg caaaaaggac tccaaaggcg ctgaagatac ccacgtggaa
     2161 caggatgccg cggatatatg ccacccaatg attgcgggtg ctttccttct cccagggcgc
     2221 catacccagc gtgaaagcat acaggatgcc gcggcgtacg tccccgcgcg ggcgggccag
     2281 gtcctgcttg aacggccggc gcataatttc attgatccgc agcaggatgc ctgccaggcc
     2341 gatcaccagg cctaaaatgg caaaaattcg cgtgatttcc agtaagctca tcatttccct
     2401 ctcatcgtta cttgaccgat atgcgcaagg caatgtcgta ggcttgctgc aaggcagcgc
     2461 tggctttggc tgagtctccc tggtcgagaa acatccgcgc cagtgttaga gaggcacgca
     2521 ccggagccgt ggcatcaccg cgcacgcgcg ctgccagggc catgcccagt gctctctcaa
     2581 agtcctgctg ggagccggtg gcaccggcga gacagcgcag aacaaccgcc ttatccgctt
     2641 cacggtcgat ctgctccagc acgcctgctg cagctgccgg atcggtctgg ctccaggcac
     2701 acgccagggg taccagggcg ccgctctccc gcaagggatc gtccgccagc actgcccggt
     2761 cccaggctgt agcgtaatca cccagattgg tcagaatctg gactttataa tagaccgagg
     2821 cagcccgttg gataagtgat gatgttcctg tcttttggat gatatcccgc agggcagcat
     2881 ctgccagggc cgggacccgg atcgtgtagg cttgctcgat atcttccagg tgggggacga
     2941 taagcgcctg gagatgcgcc tgttcataga ggtcgctgac cgactggctg gtctcccagg
     3001 cggcggcggg ctgacccaga cccagctgcg ccagggcgcg ggctgccggg aattcagcgg
     3061 cgatatcatc caggataaac gcatccccgg attggaccgc cagccggctc aaggcaaagg
     3121 caaagctgcc tgccgtgagc tggtcctgcg ccgcgaccag ggctgcatgg gcttcatcga
     3181 acaactcccc ctgaccggca gccagggcga tgcgtgccag ggaacctgct ttttgaacag
     3241 gatcgcggac caggcgagcg gcgtcagccg cagaagcata atcgcccagc tcgcggtatc
     3301 cccaggcacg gactgccgga tcgttaacct tttccattac aagcgcggcg cgggctggat
     3361 cgagctccgc ccagacgagg gcgatggtgc gcaggtcgta atcacgggct ctgcccagcg
     3421 cgccgccagc tgtatcttcg gcagcctcca aaagctggcc agctctggca ggatcgaccg
     3481 ggagccaggc ttcggctgct gcccgtaagc cccaggctcg cgagcgcatg gcatccgctt
     3541 cgctggcagt cagggctgcc t
//
LOCUS       DATMXK010000226         2097 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_167975, whole genome shotgun sequence.
ACCESSION   DATMXK010000226 DATMXK010000000
VERSION     DATMXK010000226.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2097)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2097)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2097
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167975"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(90..860)
                     /locus_tag="VN363_04225"
     CDS             complement(90..860)
                     /locus_tag="VN363_04225"
                     /inference="COORDINATES: protein motif:HMM:NF016176.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YceI family protein"
                     /protein_id="HWQ83747.1"
                     /translation="MKRIITLSILLSILLAACSSAPQNTTLPDATTSAPLATGIVINN
                     DPTATDSNLPEVSPTGPAPTAESTPTEPVATGPVEYQIDATGSSVSYEVGETFFNQNN
                     RINVAIGVTNGISGSIQLDPVNPQNTTLSDITVDVSQFASDSGRRDRAIQDRFLESGR
                     YPTATFSPTQITGLPEQYIVGEMLSFQVTGDLTVREAARPVTFDVQASLNEGILGGQA
                     TTTILMSDFGVGPIQMAGILGTEDEVKISFTFVARPVN"
     gene            complement(1002..1838)
                     /gene="prmC"
                     /locus_tag="VN363_04230"
     CDS             complement(1002..1838)
                     /gene="prmC"
                     /locus_tag="VN363_04230"
                     /EC_number="2.1.1.297"
                     /inference="COORDINATES: protein motif:HMM:TIGR00536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA];
                     GO_process: GO:0018364 - peptidyl-glutamine methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor N(5)-glutamine
                     methyltransferase"
                     /protein_id="HWQ83748.1"
                     /translation="MTPLAVHLARLKDRLKFTDTPGLDAQVLLAHILGVDRSRVLSHP
                     EAELDHSQEQALEAAAARLESGEPLPYVLGEWEFYGMSFHLTPQVLIPRPETELLVEL
                     AVDWLAGHPDRRSTADIGTGSGCIAVALAVQVTDLKVMAAELSAPALQVAGENILHYR
                     LQDRVGLVQSDLMDALEGPFDLICANLPYIPTETLHSLAVYQHEPTLALDGGKRGLDL
                     IARLLKQAPRRLAPGGLVLLEIEAPQGVVVSDLARQAFPGGVIEVVPDLAGHDRVVSI
                     LS"
     gene            complement(1866..>2097)
                     /locus_tag="VN363_04235"
     CDS             complement(1866..>2097)
                     /locus_tag="VN363_04235"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559389.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="peptide chain release factor 1"
                     /protein_id="HWQ83749.1"
                     /translation="KQREAREENRRSQVGSGERSEKIRTYNYPQSRVTDHRINLSSYN
                     MPAILDGDIDQFIDELATRDEADRLSAVGLED"
BASE COUNT          498 a          555 c          547 g          497 t
ORIGIN      
        1 ttctggcaca aatgaacaat ccaggcttgg tgctggatgt tttctaagaa ttctggtggc
       61 aaagcctgcc tcctgttgcg tgttcatcct taatttactg gacgagccac aaaagtaaag
      121 ctgattttga cctcgtcttc cgtgcccagg attccagcca tctggattgg accgacgcca
      181 aaatcactca tcaagatcgt ggtagtcgcc tggcccccca gtataccttc attcaacgag
      241 gcctgtacat caaacgttac cggcctggcc gcctcacgca ccgtcaggtc accggtaact
      301 tgaaagctaa gcatttctcc aacaatatac tgctctggta agccagtaat ttgagttgga
      361 gaaaatgtag ccgttgggta acgcccggat tccagaaaac gatcctggat tgcccggtct
      421 cgacgcccgc tatcggatgc aaactggctg acatctacgg taatatcgct gagggtggtg
      481 ttctgagggt ttaccggatc aagctggatg gatccgctga ttccattcgt cacaccgatc
      541 gccacattaa tgcgattatt ttgattgaaa aaggtttcac ccacctcgta actcaccgat
      601 gacccggttg cgtcaatctg atattcaaca gggcccgtgg caaccggctc cgtgggagtg
      661 gattctgcgg tcggggcagg accggtaggg ctgacttccg gcaggttcga gtctgttgca
      721 gtaggatcgt tgttaatcac gattccagtc gcaagtggtg cactggttgt agcatctggt
      781 aatgttgtgt tctgaggcgc gctgctgcaa gcggcaagca gaatggacag cagaatggaa
      841 agcgtaatga tgcgtttcat ggcggaattc tccttgttca tgcaaatttc ctgcgcacaa
      901 attacccggg ccaaataatg cgaccaattc gagggcaatg atagatttta cgtgaataac
      961 ggggatttag attgagatca aggggtactt ctcatcgaag tctaagaaag gatacttaca
     1021 acccggtcgt gtcctgccag atcaggtaca acttctataa ctcccccggg gaaggcttgc
     1081 cgcgccagat ctgacactac aaccccttgg ggggcttcaa tctccagcag gaccaaccca
     1141 ccaggtgcca gccgcctggg agcttgcttc aaaaggcggg cgatcaaatc caggccgcgt
     1201 ttgccgccat ccagcgccag ggttggctcg tgctggtaca ctgccagact gtgtaaagtt
     1261 tctgttggaa tatacggaag attggcacaa atcagatcga aaggaccttc cagagcatcc
     1321 atcagatcag attgaaccag ccccacacga tcctggagcc ggtaatgaag gatattctca
     1381 cctgccacct ggagagccgg agctgataat tcggcagcca tcaccttaag gtctgttacc
     1441 tgaacagcca atgccaccgc gatgcaaccc gagcctgtgc caatatcggc tgttgatctt
     1501 cgatccggat gtccagccag ccaatcgaca gccaattcaa ccagcagctc agtttccggg
     1561 cgggggatca acacttgagg agtgaggtgg aacgacattc cgtaaaattc ccactcccct
     1621 aatacatagg gcagcggctc gccgctttcc aatctcgctg cagcagcttc taaggcttgc
     1681 tcttgagaat gatccagctc ggcttctgga tggctgagca cccgggagcg gtccaccccc
     1741 aggatatgcg ctaacaggac ctgggcatct aacccgggag tatcggtgaa tttcaggcga
     1801 tcttttagcc tggctaagtg gactgccagc ggggtcaaag tatattattc agggaaatat
     1861 tattctcagt cttccagccc caccgcagac aggcgatcag cttcatcgcg ggttgccagc
     1921 tcatcaataa attgatcaat atccccgtcg aggatcgccg gcatattata ggaagaaagg
     1981 ttaatgcggt gatcggtcac gcgcgattgg gggtaattat aggtgcgaat cttttcggaa
     2041 cgctcaccgc tgccgacttg agagcggcgg ttttcctcgc gtgcttcccg ttgtttc
//
LOCUS       DATMXK010000227         2053 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_46747, whole genome shotgun sequence.
ACCESSION   DATMXK010000227 DATMXK010000000
VERSION     DATMXK010000227.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2053)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2053)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2053
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46747"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..198
                     /locus_tag="VN363_04240"
     CDS             <1..198
                     /locus_tag="VN363_04240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83750.1"
                     /translation="VLSEAEMLAGICRYFEMPDTKRLDEKERMTRLVADKSDIQNTIQ
                     AVRNVIEQAGAAWQAPEASRR"
     gene            195..1430
                     /locus_tag="VN363_04245"
     CDS             195..1430
                     /locus_tag="VN363_04245"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024831.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ83751.1"
                     /translation="MKIGYLLEGSEEVRRPPYNGPANHVRQIIRELLNMGHIVRLLAR
                     IDGQIWYSDDLKLFVPIESLPPDRGWQRLVEKGVRRGQSAFRLPYWGYFESRRFAQAC
                     QSVLGGYDIFLERFTWMNYGGLLAARAMDIPWVAEYNGDPLADLEAKQADPHGLQRII
                     SVWLTKWTLNQAEHVIATGDGWRANCSENWGVSPEEITVIENGTDLLQLLDRPMLKSF
                     QSPAFQQPVQVVYLGGFYPWHGIHILLRAMCTAIAAGVQFRLVLIGAGSGMEEAQEMV
                     RASGLGEYVEFTGRLTAQAYAPYLAQADIGVSPYCGWKEYSGLKLFDYKAAGLACIAS
                     GENGQPATLKHGETGWIVPPCDEEKLTQALVHLADQPELRRKLGQAARLEAENYHGWD
                     HTTDKLENILENIIKMREN"
     gene            1430..>2053
                     /locus_tag="VN363_04250"
     CDS             1430..>2053
                     /locus_tag="VN363_04250"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWQ83752.1"
                     /translation="MSPDFQPGLISIMMPVYNAEKFIEQAIESVLAQTYPNFELIIVN
                     DGSTDRTPELAVKYSDPRIRVIHQSNGGESIARNTALRHMHGEYVAFLDADDMFLERH
                     IELGIAYLNAHPDCDGVYSDGYHFDAHGNRSQSLSSNRRGPFEGFIFGEVARASDVFG
                     PPICVILRRGIITRFHLEYDPEIVIGPDWDFFIQYAELGRFGSVPDFT"
BASE COUNT          501 a          479 c          575 g          498 t
ORIGIN      
        1 gtcctcagcg aggcagaaat gctcgccggg atctgccggt attttgaaat gccggacacc
       61 aaacgattgg atgaaaaaga acggatgacc cgtttagtcg ccgataaatc ggacatccag
      121 aataccatcc aggcagtcag gaatgttatt gaacaggcgg gcgcagcctg gcaggccccg
      181 gaagcttctc gacgatgaaa atcggctatc tgttggaagg cagcgaagaa gttcgccggc
      241 ctccatacaa cggaccggct aaccatgtcc gccagatcat ccgggagttg ctaaacatgg
      301 gacatatcgt aaggctgctg gctagaatcg atggtcagat ctggtattcg gatgatctca
      361 aactattcgt cccaatcgag agtctacccc cggatcgtgg ctggcagcga ctggttgaaa
      421 agggtgtccg gcgagggcag tccgccttca ggctgccgta ttggggttac ttcgagagcc
      481 ggcgctttgc tcaagcctgt caatcagtat tggggggtta tgatattttt ttggagcgat
      541 ttacctggat gaattatgga ggtctgcttg cagccagggc aatggatatt ccctgggtgg
      601 cagaatataa cggcgatccg ctggcggacc tggaggcaaa acaggcggat ccccatggat
      661 tgcaacggat aatatcggtc tggctcacaa aatggaccct caatcaagct gagcatgtca
      721 ttgcgacggg tgatggctgg cgggcgaact gcagcgaaaa ttggggtgtg agtccggagg
      781 agatcacggt gatcgagaac ggcacagatc tgctgcagct gctggaccgg cccatgctca
      841 agtcgtttca atcacccgcc tttcaacaac cagttcaggt ggtttatctg ggtgggtttt
      901 atccctggca tggtatccat atcttgctga gggcgatgtg cactgcgatc gctgccggag
      961 tacagttcag gctggtatta atcggtgccg gaagcggcat ggaagaagcc caggaaatgg
     1021 taagagcctc cggccttgga gaatacgttg aatttactgg tagattgact gcccaggctt
     1081 atgcacccta ccttgcccag gcggatatcg gggtttcacc ctactgtggg tggaaagaat
     1141 attctggttt gaagctgttt gattataaag ctgcgggctt agcatgtatt gcttcgggtg
     1201 aaaatgggca gcctgccacg ctgaagcacg gcgagacagg ctggatcgtc cctccgtgtg
     1261 atgaagaaaa attaacccag gcactggtgc atctggctga ccagcctgaa ttacgccgta
     1321 aacttggaca ggctgcacga ttagaagctg aaaattacca cggttgggat cacacaaccg
     1381 ataaactgga aaatattctc gaaaatataa tcaaaatgag ggaaaattaa tgtcaccaga
     1441 cttccagcct ggattgatca gcatcatgat gcctgtctat aatgcagaga agtttattga
     1501 acaggccatc gagagtgtgc tggctcaaac ctatccgaat tttgaattaa ttatcgtcaa
     1561 cgatggctca actgatcgca cacctgagct tgcagtgaag tacagcgatc ctcgcataag
     1621 ggttattcac caatccaatg gcggagaatc aatagcccgc aacaccgctc tgcggcatat
     1681 gcatggggaa tacgttgctt ttctggatgc cgacgatatg ttcctggaac gccatattga
     1741 gctgggcatc gcctatttga atgcccatcc agactgcgat ggggtttatt cggatggata
     1801 tcactttgat gcgcacggaa atcgctcaca atccctgtca agcaaccggc ggggtccttt
     1861 tgaagggttc atttttggcg aagtcgcccg ggcatccgat gtatttggtc ccccgatctg
     1921 tgtgatctta aggcgcggaa tcattactcg tttccacctg gaatatgatc ccgaaattgt
     1981 gatcggaccg gattgggatt tctttatcca gtatgctgag ttgggccggt ttggttctgt
     2041 acccgatttt acg
//
LOCUS       DATMXK010000228         2287 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12194, whole genome shotgun sequence.
ACCESSION   DATMXK010000228 DATMXK010000000
VERSION     DATMXK010000228.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2287)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2287)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2287
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12194"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1040
                     /locus_tag="VN363_04255"
     CDS             <1..1040
                     /locus_tag="VN363_04255"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ83753.1"
                     /translation="IKYTPADGTPSLKKAIIQYTEDNYNRLPAPENVIVSAGAKQSLY
                     NVLFSILNPQDEVIVVAPYWVSYPEMIKMIYGIPVIVTPEDGSFYPCMADIERATSSY
                     TRAVIINSPNNPSGVLYPESFIAEIVEYCEKRKIYLIMDDIYHKLVFDNKKWTPAYQY
                     TQQNIDNSMVIVLNGVSKLYGMTGFRIGWVVANRQLVEVMTNVQSQTSSCTSMVLQSA
                     AEGALTGMQSVVEGLRLTIENNRNIIVQELKTFSGVRASNPDGTFYILPDFRAYSNKS
                     VELSQFLLHKALVVTVPGKEFGMEGHLRLSYSGSVKDVTTAIERMKWALDPNTSNEIY
                     IGDRKLIRDWL"
     gene            1037..>2287
                     /locus_tag="VN363_04260"
     CDS             1037..>2287
                     /locus_tag="VN363_04260"
                     /inference="COORDINATES: protein motif:HMM:NF013461.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoenolpyruvate carboxykinase (ATP)"
                     /protein_id="HWQ83754.1"
                     /translation="MNNILDIKTPAAAQAAALKADYGLDNLGLGNLGKVYWNLPSEAL
                     CEEAVFRNEGKITRLGPLVVNTGQLTARAANDKVIVREPGTEGQIWWGQYNRPFDTAK
                     FNDLFNRVQGFLQGRDVFVQDCFAGADPNYRLSVRIITEFAWHSMFARNMFIPPRTAD
                     EYRRHIPDFTVIAVPSFKAFPQIDGTNSNTVIVINFDQKLCIIGNSGYAGEIKKSIFT
                     IMNYLLPLEGVMPMHCSANVGQDGSVALFFGLSGTGKTTLSADPNRRLIGDDEHGWSD
                     EGIFNFENGCYAKVIALSPSAEPEIYACTERFGTILENVVYDPITRYIDLDDDHFTEN
                     TRASYPLEYIANALPEKRAGHPKNVIMLTCDASGVMPPIARLSPDQALYHFISGYTAK
                     VGGTELGLGAEPEITFSTCFGGPFM"
BASE COUNT          534 a          701 c          574 g          478 t
ORIGIN      
        1 atatcaaata caccccggcg gatggcacgc catccctcaa gaaagcgatc atccagtaca
       61 cggaagataa ttacaaccgc ctgcccgccc ccgaaaatgt catcgtatcc gccggcgcca
      121 agcagtcact ttataacgtc ctcttcagca tcctcaaccc gcaggacgaa gtgatcgtgg
      181 tcgcccccta ctgggtcagc tatccggaaa tgatcaagat gatctacggc atccccgtga
      241 tcgtcacccc ggaagatggc agcttttacc cctgcatggc ggatatcgaa cgcgccacca
      301 gctcctacac ccgggcagtc atcatcaaca gccccaacaa tccctccgga gtactttacc
      361 ctgaatcgtt tatcgccgag atcgtggaat actgtgaaaa acgcaagatt tacctgatca
      421 tggacgatat ctatcacaag ctggtattcg ataataaaaa atggaccccc gcctaccagt
      481 acacgcagca gaacatcgat aattcgatgg tgatcgtcct gaatggggta tccaagctct
      541 acggcatgac cggctttcgg attggctggg tcgttgccaa ccgccagctg gtcgaagtga
      601 tgaccaacgt ccagtcgcag acctcctcct gcacctcaat ggtgttacag tctgccgctg
      661 aaggcgccct gaccggtatg cagagcgtgg tcgaaggcct gcgcctgacc atcgaaaata
      721 accgcaatat tatcgtccag gagctcaaga cattctccgg agtgcgggct tctaatccgg
      781 atggcacgtt ctatatcctg cctgattttc gagcttactc caacaaatcg gtcgagttgt
      841 cccagtttct gctccacaaa gccctggtag tcactgttcc aggcaaggaa ttcggcatgg
      901 aaggacatct gcgcctgagc tactccggct cggtcaagga tgtaaccacc gccatcgaac
      961 gcatgaagtg ggccttagac cccaatacct cgaatgaaat ctacattggc gaccgcaagc
     1021 tgatcaggga ctggctatga acaacattct ggacattaaa acccccgcgg cagcccaggc
     1081 tgcggccctg aaagccgatt acggcctgga taatctgggt ctgggcaacc tgggtaaagt
     1141 atattggaat ctgcccagcg aagcgctgtg tgaagaagct gtctttcgca atgaaggtaa
     1201 gatcacccgc ctgggaccgc tggtggtcaa taccgggcag ctgaccgccc gggctgcgaa
     1261 tgataaagtg attgtgcgcg agcctggcac tgaagggcag atctggtggg ggcagtacaa
     1321 ccgccccttc gacaccgcca aattcaacga cctgttcaat cgtgtccagg gcttcttgca
     1381 gggccgggat gtattcgtcc aggactgctt cgccggcgcg gatccaaatt accgcctgtc
     1441 ggtgcggatc atcacagaat ttgcctggca cagcatgttt gcccgcaata tgttcattcc
     1501 cccgcgcaca gccgacgaat accgccgcca tatcccggat ttcacggtca tcgctgtccc
     1561 atctttcaaa gccttccccc agatcgatgg caccaattcc aacacggtga tcgtgatcaa
     1621 tttcgaccag aagctgtgca tcatcggcaa ttccggctac gccggtgaga tcaagaaatc
     1681 gatcttcacg atcatgaatt acctgctccc cctggagggc gtaatgccca tgcactgctc
     1741 ggccaatgtc ggccaggatg gcagtgtcgc cctgttcttc ggcctctctg gcaccggcaa
     1801 gaccaccctg agcgccgatc caaaccgccg cctgataggc gatgacgagc acggttggag
     1861 cgacgaaggg attttcaatt tcgagaatgg gtgttacgcc aaggtcatcg ccctctcacc
     1921 gtcggctgag ccggaaattt atgcctgtac agagcgcttt gggacgatcc tggaaaacgt
     1981 cgtttacgat cccattaccc gctacatcga tctggacgac gatcacttca ccgagaatac
     2041 ccgtgcatct tatcccctgg agtatatcgc caatgccctc ccggagaaac gcgccgggca
     2101 tcccaaaaat gtgatcatgc tcacctgcga cgcctccgga gtgatgccgc ccatcgcccg
     2161 cctctcaccg gaccaggcgc tgtaccattt catctccggc tacaccgcca aagtcggcgg
     2221 aacagagctc ggattaggcg ccgagccgga aatcaccttc agcacctgct tcggcgggcc
     2281 gttcatg
//
LOCUS       DATMXK010000229         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_32230, whole genome shotgun sequence.
ACCESSION   DATMXK010000229 DATMXK010000000
VERSION     DATMXK010000229.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32230"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1039)
                     /locus_tag="VN363_04265"
     CDS             complement(<1..1039)
                     /locus_tag="VN363_04265"
                     /inference="COORDINATES: protein motif:HMM:NF024912.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0097367 - carbohydrate derivative binding
                     [Evidence IEA];
                     GO_process: GO:1901135 - carbohydrate derivative metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SIS domain-containing protein"
                     /protein_id="HWQ83755.1"
                     /translation="MCGIFGIVKKEDSQLGPILIDAGRRLSYRGYDSVGCATLAGDGT
                     IDLRKDVGMVDEVASRYSFAQMTGQRGILQLRWATFGEPSQVNAQPHLDSDGDLVGAH
                     NGNVVNNVELREEFINEGMLVRSTNDGESCVHAVERYLDRGYEFIPAIGCAYQDLQGD
                     YAFVIGRAGEDRLYALKKGSGLVVGLAEDSTCVSSDLPSILPLTRKILRVYDGEIVVL
                     YPDHAELYRVSDGSQITREPEIIQESMEAAQKGGYPHFMLKEIPEQPAVARELLHLLD
                     ASAAIDAMVARLQKARNIYLIGCGTSYHACILGSVYLARLAGRTAIPVLAPQFVAQYA
                     PAVSSEDVGIFV"
     gene            complement(1217..>2088)
                     /locus_tag="VN363_04270"
     CDS             complement(1217..>2088)
                     /locus_tag="VN363_04270"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HWQ83756.1"
                     /translation="STPIPCNQAEFVRDVTIPDNTELQPGQSFTKTWRLRNIGSCAWT
                     TGYSLVFLSGDAMGGASADMPQRVEPGSSIDLSVNLIAPNTAGTYKGNWMLRDRDGRF
                     GIGTGASNPFWVQIKVVQTATSGIVYNFASNYCSADWTSGAGDLDCPGDDSDDEGFVI
                     RLTEPDMEHRQENEPGIWTRPEETNDGYIRGIFPAFRILDDDRFRADIGCLDGSEDCD
                     VIFELSYRIGGGGLDRLGEWHEVDDNAVTRIDIDLSGLAGRDVEFVLTVRSNGSPADD
                     DAFWLMPQIYRPD"
BASE COUNT          451 a          610 c          610 g          417 t
ORIGIN      
        1 tgacgaatat cccgacatcc tcggagctga ctgccggggc atattgagca acgaactgtg
       61 gggccagcac tgggatggca gtccggcccg ccaggcgcgc caggtaaact gagcccagga
      121 tacaggcatg gtagcttgtg ccgcagccga tcaggtagat attgcgggct ttttggaggc
      181 gagcaaccat ggcgtcgatc gctgccgaag cgtccagcag gtggagcagc tcacgggcaa
      241 ccgcgggctg ctcggggatc tctttgagca tgaaatgcgg gtagccgcct ttttgggcgg
      301 cttccatgct ctcctggatg atttcgggct cacgggtgat ctggctgccg tcacttaccc
      361 gatacagctc ggcgtgatcc ggatacagca caacaatctc cccgtcatag acacgcagga
      421 tcttccgggt caggggaagg atcgaaggga gatcggagga tacacaggta gaatcttcgg
      481 ccagccccac caccagcccc gaacctttct taagggcgta aagcctgtct tcaccggccc
      541 gcccaatgac aaacgcgtaa tcgccctgca ggtcctgata ggcgcagccg atggcgggaa
      601 tgaattcgta gccgcgatcc agatagcgct ccacggcatg cacgcaggat tcgccatcgt
      661 tggttgaacg caccagcatg ccttcgttga tgaactcctc gcgcagctcg acattgttga
      721 cgacattgcc gttgtgcgct cccaccagat cgccatccga atcgagatgg ggctgggcgt
      781 ttacctgtga aggctcgccg aaagtcgccc agcgcagctg gaggattccc cgctgcccgg
      841 tcatttgtgc aaagctgtag cggctggcga cttcatcgac cataccgacg tctttgcgca
      901 gatcgatcgt tccgtctccc gcaagggttg cgcagccaac agaatcataa ccgcgatagg
      961 acaatcgccg cccggcatcg atcaagatcg gacccagctg gctgtcttct ttttttacga
     1021 tcccaaaaat accgcacaca ggctacctcg gataaataaa gttggtccgg ctcaggagga
     1081 atggaaagga tcacgttgat ccgagacggc ccgggcctat tatacaggtg aaattctaaa
     1141 tctgagaaag caagccggcc tgccaggaaa attttctggc aggccgggga gaatctgtga
     1201 ggtggaaagc tctggctcag tcagggcggt agatttgcgg catgagccag aaggcgtcat
     1261 cgtccgccgg gctgccgttg ctgcggacgg tcaggacgaa ttcgacatcc cgcccggcca
     1321 gcccactgag atcgatatcg atgcgggtta cagcattgtc atcaacttca tgccattcgc
     1381 ccagcctgtc taatccgccg ccgccaatgc ggtaggacag ctcgaagatc acatcgcagt
     1441 cttcgctgcc gtccaaacag ccgatatcgg ctcggaagcg gtcatcatcc aggatgcgga
     1501 aggcggggaa gatcccccgg atgtagccat cattggtctc ttcagggcga gtccagatgc
     1561 cgggctcatt ttcctggcgg tgttccatat ccggctcggt caggcggatc acgaaacctt
     1621 cgtcgtcaga atcatcgccc gggcagtcca gatcgccggc gccgctggtc caatctgccg
     1681 aacaataatt gctggcgaaa ttgtaaacaa tgccactggt ggcagtctga acaactttga
     1741 tctggaccca gaagggatta ctggccccgg tcccaatccc aaaacgtcca tcccggtcgc
     1801 gcagcatcca gttgccttta taggtgccgg cagtattggg tgcaatgaga ttaaccgaca
     1861 ggtcgatact ggagccgggc tcgacccgct gcggcatgtc cgcactggcg ccacccatgg
     1921 catccccgct taaaaagacc agcgaataac cggtcgtcca ggcgcaggag ccgatgttcc
     1981 tgagccgcca ggtttttgtg aatgactggc cgggctgcag ctcggtatta tccgggatgg
     2041 tcacatcccg gacgaattca gcctgattgc aggggatcgg agtcgacg
//
LOCUS       DATMXK010000230         7317 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_47198, whole genome shotgun sequence.
ACCESSION   DATMXK010000230 DATMXK010000000
VERSION     DATMXK010000230.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7317)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7317)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7317
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47198"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..734
                     /locus_tag="VN363_04275"
     CDS             <1..734
                     /locus_tag="VN363_04275"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558900.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83757.1"
                     /translation="FIPVMMIAGILAGGLWGFIPGFLKARLNVNEIITTLMMNYIAVA
                     WNNYFIFAIWTEGGFQMSPVFPKNAWLPRLADYAIQVPLFRGLTVHLGLIMALLAAVV
                     VWFILYRSRWGYEIRLIGDNPRAARYAGLNITRNMVLVMMLSGGLAGLAGMSEITGVV
                     HRLQGAISPGYGFTGIIIAWLAKLNPFAVILVSILFGGLILAGREIQPSGIPRMIQGI
                     ILVCLIASDFLIRYRVRIVRVNGEA"
     gene            738..1706
                     /locus_tag="VN363_04280"
     CDS             738..1706
                     /locus_tag="VN363_04280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558899.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83758.1"
                     /translation="MDLIIILQAGVATGTILLFATIGEIIAERSGVMNLGVEGMMLIG
                     AMSAFSIAVSTGNPWLGLIVAMLVTGLFSQIHAFITITLQADQVVSGLALSFLGSGIS
                     VVLGEGLSKAGAISLLPSFSIPLLSQIPILGPIFFTNQSIMVYAGYLLIPLVWYYINR
                     TRPGMHLRAIGEYPAAADAVGINVFRLRYFYVFVGGVLAGLAGATISLAISPGWFSEL
                     TTAGQGWIAIGLVIFAQWNPIRAAAGSYAFGALRRLILDIQGPTMLFGLANPFYYNPY
                     WGFFLQMLPYAFTVIVLVIGSREAMRKRLGTPAALGTPYIRGERGL"
     gene            1711..2961
                     /locus_tag="VN363_04285"
     CDS             1711..2961
                     /locus_tag="VN363_04285"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558897.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWQ83759.1"
                     /translation="MEKYVGYRCSVCGEEYDPAEVTYTCPRDGGNLDVILDYNTIRKF
                     IQPEMIFHQDEPSLWRYLPLLPIQDPGGRGTPLHAAGWTPVFRPPRLAAELGVDKLWI
                     KDESRNPTASFKDRASAIVVGRAREIQAEVVVTASTGNAGAALAGMSAAIGQKAIIFA
                     PKTAPPAKIAQLIVFGAQVILVDGSYDDAFDLTIQAAQEFGWYCRNTGYNPFTAEGKK
                     TAAFEIWEWLTIDQIPHSPSRPLTVFVSVGDGNIISGIHKGFKDLLALGWLEASPRLI
                     GVQAEGSAAIANAYTAGTEQITPVRATTLADSISVDLPRDGLRAVRAATQTGGAYITV
                     SDPEILAAIAALGPCGIFAEPAGAAAYAGLVKALQVGLVNPDDPILVLNTGSGLKDVK
                     AAMQATRQAPVIEPDLQAVKNLLK"
     gene            2968..3948
                     /locus_tag="VN363_04290"
     CDS             2968..3948
                     /locus_tag="VN363_04290"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWQ83760.1"
                     /translation="MSEPVFSILAPIFNERENLPELYQRVSQVMDQTGEAWELLLIDD
                     GCTDGSTEIIRELGAQDGRVRPIIFARNFGHQIAVTAGLDHSRGRAVVIIDSDLQDPP
                     EVILDLIAKWREGYEVVYAVRAEREGESWFKLFTASLFYRVIFRITDIEIPLDTGDFR
                     LLDREVVNVLNRMRERHRFLRGMSAWVGFKQVGVPYRRAARHAGETKYPFNKMLRLAI
                     NAITGFSYFPLQLATYLGFICAGISIVAIPIVVITRLTGSQAFFGQATTLIAVLFLGG
                     VQLISLGILGEYIGRLYDEAKGRPLYIVRQEQQLPTQNQQSTTNPPTEVR"
     gene            3964..4533
                     /locus_tag="VN363_04295"
     CDS             3964..4533
                     /locus_tag="VN363_04295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83761.1"
                     /translation="MLIPPSALVLVAVINQPRDLEIARLLGWYRIPLGRAPKVVAVDY
                     LAFYQTAAFGAEKWRIQTVAPLLGHELTTRAELLRDEPDHPRAQAMYYKLQLGPLQSL
                     PQPIIAKNWRRITFLYTTGEYLLNAETVNDLVIETEERQSLWQALRERATRAQKYGER
                     QQPEIPPLNVDPAFLAALLGLSNVDHSEK"
     gene            4584..5690
                     /locus_tag="VN363_04300"
     CDS             4584..5690
                     /locus_tag="VN363_04300"
                     /inference="COORDINATES: protein motif:HMM:NF021539.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2270 domain-containing protein"
                     /protein_id="HWQ83762.1"
                     /translation="MADNNLPPQESTRGNEKPDNTAEGDGPQPVWTYRGYKLRSSEFT
                     TAMVHQFRAEVQRANVWRQRLDTTTNWAVVTTGAVISIAFTGETAPHLVIILNLLLVT
                     FFLSIESRRYRYFELWSYRIRLMETDFFAAMLVPPFHPAADWAESLAENLLHPHYPIT
                     YTEALGRRLRRNYLWIYGVIYLAWFAKIWLQPTTAINWAHVVRRASIGGFSGQLIMGV
                     MIGIGVFLALFAVITIRLQEATGEVLPRYAGLPEEFASVFSKTETTHPAEHAWYRKGR
                     RRRQFMTIIITDQAEAVSARIIKDMARGVTALTGKGMYTGKEHQILLVALTTTEIPHM
                     KELVRSVDENAFVIVSPAQEILGRGFLPLEEIQY"
     gene            5711..7126
                     /gene="hydA"
                     /locus_tag="VN363_04305"
     CDS             5711..7126
                     /gene="hydA"
                     /locus_tag="VN363_04305"
                     /EC_number="3.5.2.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR02033.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004157 - dihydropyrimidinase activity
                     [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropyrimidinase"
                     /protein_id="HWQ83763.1"
                     /translation="MKTLIKGGTLITAAETFIADILIDGEKIIQLGVDLNDPDAQLIP
                     ADGRLVMPGGVDAHTHFDLPMSETVSSDDHYTGHKAAAFGGTTTVIDFVPQDLPTLRG
                     SVAAWHSKADPKAAIDFGFHMNITALTPQIEAEISTLRDMGITSLKVFTAYNNRLRLA
                     DDEIFRVMRIAKENNLLTMVHAENGDMIELLVAEAIQAGHTSPEWHARTRPAWGAVEA
                     SLRSAALAAQAEAPVYIVHMNAAGEADMLEYARQKGLPVMGETCPQYLFFSIENLQRE
                     DGAKWVCSPPVRSTADNARLWKGLQSGAIQVIATDHCPFFYDGTSPIQYEGQPVAIPG
                     KELGQGDFTRIPNGLPGVGDRMPVLWTYGVRSGRLTPNQFVALNCTNPARIFGLYPQK
                     GALVPGADADLVIWDPERRLTYGTAYAQHRTDYNLYEGWELVGYPEKVFLRGNLIVID
                     GEWRGQPGMGRYLHRQGATVL"
BASE COUNT         1522 a         2076 c         2012 g         1707 t
ORIGIN      
        1 tctttatccc ggtcatgatg atcgccggga tactggccgg ggggctgtgg ggtttcatcc
       61 cgggttttct aaaagccagg ttgaacgtga acgaaatcat caccaccctg atgatgaact
      121 acatcgccgt cgcctggaac aattatttta tcttcgccat ctggaccgaa ggtggttttc
      181 agatgtcgcc ggtattccct aagaatgcct ggttgccccg cctggctgat tatgccattc
      241 aggtccctct ctttcgcggg ctgaccgtac accttgggtt aatcatggcg ctcctggctg
      301 cggtggtggt ctggttcatc ctgtatcgca gtcgctgggg gtacgaaatt cgcctgatcg
      361 gcgacaatcc tcgggctgcc agatacgccg ggctgaatat cacccgcaac atggtcctgg
      421 ttatgatgct gtcggggggt ctggccgggt tggcgggtat gtccgaaatc acgggagtcg
      481 tccatcgctt gcagggagcg atctcgcccg gatatggttt cacggggatt attatcgcct
      541 ggctggcaaa gcttaatccg tttgccgtaa tcctggtttc gattctgttc ggaggattaa
      601 tcctggcggg tcgcgagatc caaccctccg gaattccgcg catgatccag ggtattatcc
      661 tggtttgctt gatcgccagc gactttttga ttcgctaccg ggtacggatt gtccgggtaa
      721 acggagaggc ctgacttatg gatttgatca tcattctcca ggctggcgta gccaccggta
      781 ccatcttgct ctttgcaacc atcggtgaga tcattgccga acgctcaggg gtaatgaacc
      841 tgggcgttga agggatgatg ctgatcggtg ccatgagcgc cttcagcatt gcggtcagta
      901 ccggcaatcc ctggctgggg ttaattgtcg ccatgctggt taccggtctc ttcagccaga
      961 tccatgcctt tatcaccatc actttgcaag ccgaccaggt cgtgagtggg ctggcgctga
     1021 gcttcttagg cagcgggatc agtgttgtat taggggaagg gctgagcaaa gccggggcca
     1081 tctcgctgct gccatccttc agcatccccc tgctctccca aatcccaatc ctggggccaa
     1141 tctttttcac caatcagagc ataatggtct atgccggtta tttgttgatt ccgctggtat
     1201 ggtactatat taaccgcacc cgtcctggca tgcacctgag agcaatcggt gaatacccgg
     1261 ctgccgcaga cgcagtcggg atcaatgtct ttcgcttgcg ttatttctat gtttttgtcg
     1321 gcggtgtttt ggcagggctg gctggcgcca ccatcagcct ggcgatttct ccaggctggt
     1381 tcagcgaatt aaccacggcc ggtcaaggtt ggattgccat cggcctggtc attttcgccc
     1441 aatggaatcc catccgcgcc gcggctggct cgtacgcatt tggtgcgctg cgccgtctga
     1501 tcctggacat ccagggacca accatgctgt tcggtttagc caatcctttc tactacaatc
     1561 cctactgggg cttcttcctg cagatgctgc cgtacgcatt tactgttatc gtcctggtta
     1621 tcggctcacg cgaagccatg cgtaaacgcc tgggcactcc ggctgcattg ggaaccccct
     1681 atattcgcgg cgaacgaggc ttataacaag atggaaaagt atgttggcta ccggtgctcg
     1741 gtatgtgggg aggaatatga ccccgctgag gttacgtaca cctgccccag ggatggtggc
     1801 aatctggatg tcatcctgga ttacaacact atccgtaaat tcatccagcc cgaaatgatc
     1861 ttccaccagg atgagccatc gctgtggcga tacctgccgc ttttacccat ccaggatccg
     1921 ggcgggcgcg gcacgcccct gcatgctgct ggctggaccc cggtgttcag acctcctcgg
     1981 ctggcagccg agctgggagt tgataagctg tggataaagg atgaaagccg caacccgact
     2041 gcctcgttta aggatcgcgc cagcgccatt gtcgtggggc gcgcccggga aatccaggca
     2101 gaagtcgtcg tcactgcctc caccgggaat gccggcgcag ctctggcagg aatgtcggcg
     2161 gcgatcggtc aaaaagcgat catcttcgcc ccgaaaaccg cccccccggc caaaattgcc
     2221 cagctgattg tgtttggcgc gcaggtgatc ctggtggacg gcagctatga cgacgccttt
     2281 gatctgacca tccaggcagc ccaggaattt ggctggtact gccgcaacac aggctacaac
     2341 ccgttcactg ccgaaggaaa gaagaccgct gcctttgaga tctgggaatg gctgaccata
     2401 gaccagattc ctcacagccc atccaggccg ctgaccgttt ttgtttcggt tggcgatggc
     2461 aacatcattt ctgggatcca caaaggcttc aaggatttgc tggctctggg ttggttggaa
     2521 gcttcaccgc ggctgatcgg tgtccaggct gaagggtctg ccgccattgc caatgcatac
     2581 acggccggca ccgagcagat caccccggtc cgggcaacca ccctggccga ttcgatctca
     2641 gtagacttgc cccgcgatgg actgcgcgcc gtgcgcgccg ccacccaaac cggaggcgcg
     2701 tatatcaccg tgagcgaccc cgaaatcctt gccgcgattg ccgccctggg tccgtgtggg
     2761 atttttgccg agccggcggg tgcggccgcc tatgcaggcc tggtcaaagc tctccaggtt
     2821 gggctggtaa atccggatga tcccatcctg gtactgaata ccggcagtgg attaaaagat
     2881 gtcaaagctg ccatgcaggc tactcgccag gctccagtaa tcgagcctga tttgcaggct
     2941 gtgaaaaacc ttctgaaata aatgactatg agcgaacctg ttttttccat ccttgcccca
     3001 attttcaacg aacgcgagaa ccttcctgag ctctaccagc gcgtctccca ggtcatggat
     3061 cagactggcg aagcgtggga gctgctgctg atcgatgatg gctgcaccga cggttcgacc
     3121 gagattatcc gcgagcttgg cgcccaggac gggcgcgtcc gccccatcat tttcgcccgc
     3181 aatttcggac accagattgc tgtcaccgcc ggtctggatc acagccgcgg ccgggctgta
     3241 gtaatcatcg attcagatct gcaggacccg cctgaggtca tcctcgacct gattgccaaa
     3301 tggcgggaag ggtatgaggt cgtctatgcg gtgcgcgccg aacgtgaagg cgaaagctgg
     3361 ttcaagctgt ttactgcgtc cctgttctat cgcgtgattt tccggatcac cgatattgaa
     3421 attcctctgg acacgggaga cttccggcta ttagatcgcg aagtggtcaa tgtgctcaac
     3481 cgcatgcgcg agcgccaccg ctttttacgg ggtatgtctg cctgggtagg cttcaaacaa
     3541 gtcggcgtac cttaccggcg ggcagcccgg catgctggtg agacgaaata cccatttaat
     3601 aagatgctgc gcctggcaat caatgccatc accggcttct cttacttccc tcttcaacta
     3661 gccacctact tagggtttat ctgtgccgga atcagcattg tagcgattcc aattgtcgtc
     3721 atcacccgcc tgactggctc gcaggccttc tttggtcagg cgacaaccct gatcgcagtc
     3781 cttttcctgg gtggggtaca attaatctct ctgggcattc tgggtgaata tatcgggcga
     3841 ctgtacgatg aagccaaggg cagaccgttg tatattgtgc gccaggaaca gcagctaccc
     3901 acccagaatc agcagagcac aacaaacccg cctaccgagg ttcgctagcg gcaaaactgg
     3961 cttatgttaa ttccaccgtc ggcgctcgta ctcgtcgctg tgatcaacca accccgggat
     4021 ctggagatcg cccgcttatt gggctggtac cggatcccac tggggcgcgc tccgaaagtt
     4081 gtcgctgtgg attacctggc cttttaccag actgccgctt tcggagctga gaaatggcgc
     4141 attcagactg ttgctcctct gctgggtcac gagcttacca ctcgggccga attgctgcgc
     4201 gatgagcccg atcatccccg cgcccaggca atgtactaca agcttcagct gggaccgctg
     4261 cagagtctgc cacagccaat aatcgccaaa aactggcggc ggatcacctt tctctacaca
     4321 accggtgaat atttgctaaa tgctgaaacg gtaaatgatc tggtaatcga gacggaagag
     4381 cggcaaagtt tgtggcaggc attacgcgag cgagcaaccc gggcgcaaaa gtacggtgag
     4441 cgccagcagc ctgagattcc cccgctgaat gtagatccgg cttttctggc ggctctgctc
     4501 gggctaagca acgtggacca ttccgaaaaa taggtagtat aaattagtta tgctcgatca
     4561 caggatttat gggagttgga attatggcgg ataataacct gcccccgcag gaaagtacgc
     4621 gtggaaatga gaaaccagac aataccgcag aaggggatgg acctcagccg gtctggactt
     4681 accggggtta taaactccgc tccagcgaat ttacgacggc gatggtgcac cagttccggg
     4741 ccgaagttca gcgcgccaac gtctggcgac agcgcctgga tacaaccacc aactgggctg
     4801 tggttacaac cggcgcagta atctcgatcg cttttaccgg agaaaccgcg ccccatctgg
     4861 tcattatcct gaacctgctc ctggttacct ttttcctttc catcgaatca cggcgctacc
     4921 gttatttcga gctgtggagt taccgtatcc gcctgatgga aactgacttc tttgcagcca
     4981 tgctggtacc tccatttcat ccagctgccg attgggccga atccctcgct gaaaacctgc
     5041 tccaccccca ctaccccatc acctatacgg aagccctggg gcgccgcttg cggcgcaatt
     5101 acctgtggat ctatggcgtc atttatttgg cctggttcgc caagatctgg ttacaaccca
     5161 ccactgcaat taactgggcg catgttgtcc ggcgggcatc gattggtggt tttagcggtc
     5221 agctgatcat gggcgtgatg attggcatcg gcgtcttttt ggctttgttc gcagtgatca
     5281 cgattcgctt gcaagaagct accggggaag tgcttccccg ctatgcaggt ctgcccgaag
     5341 agttcgcctc cgttttcagt aaaactgaaa ccactcatcc ggcggagcat gcctggtacc
     5401 gaaagggtcg ccggcggcgc cagtttatga ccataatcat tacagatcag gccgaagccg
     5461 tttcagcgcg gattattaag gatatggcca ggggtgttac tgcgctaaca ggcaagggga
     5521 tgtacaccgg aaaagaacac cagatccttc tggttgctct gaccacaacc gagattccgc
     5581 atatgaagga attagtgcgc tcggtggatg aaaatgcctt tgtaattgtc tctcctgccc
     5641 aggaaatatt agggcgtggt ttcctacccc tggaagaaat ccagtattag ccgttgcgag
     5701 agggatagcc atgaagacct tgattaaagg cggtacgctg atcactgcgg ctgagacctt
     5761 tatcgctgat atcctgatcg acggcgaaaa aattatccag ctgggtgtcg atctcaatga
     5821 tccggatgct cagctaatcc cggcggatgg caggctcgtt atgccgggcg gggtggatgc
     5881 gcatacccat tttgacctgc caatgtccga gaccgtttcc agcgacgacc attataccgg
     5941 gcataaagca gccgcttttg gcggcacgac caccgtgatc gatttcgtcc ctcaggattt
     6001 gcccaccctg cgcggatcgg ttgctgcctg gcatagcaag gctgatccta aagccgccat
     6061 tgattttggc ttccacatga atatcaccgc attgacgccc cagatcgaag ctgaaatttc
     6121 cacgttgaga gatatgggca tcaccagtct caaagttttt accgcctaca ataaccggtt
     6181 acgtctggct gatgatgaaa tttttcgtgt gatgcgcatc gccaaagaga acaacctgct
     6241 gaccatggtg cacgctgaaa acggggacat gattgagctg ttggtagctg aggctattca
     6301 ggccggacac accagccctg aatggcatgc tcgcactcgc cctgcctggg gcgccgtaga
     6361 agccagcctg cgcagcgctg ccctggctgc ccaggcagaa gctcccgttt acattgtcca
     6421 tatgaatgcc gccggtgaag cagacatgct ggaatacgct cgccagaaag gcctccccgt
     6481 gatgggcgag acctgtccac aatacttatt cttttccatc gagaatttac agcgtgagga
     6541 tggcgccaaa tgggtctgct caccgcctgt gcgcagcact gcagacaatg cccgtctttg
     6601 gaaagggctg cagtccggcg ctatccaggt gattgccacc gaccactgcc ccttcttcta
     6661 cgatggaacc agcccgatcc agtatgaagg ccaaccagtg gcaattccgg gcaaggaact
     6721 ggggcaaggt gattttaccc gcatcccgaa cggcttacct ggggtcggtg accggatgcc
     6781 ggttctctgg acgtacgggg tgcgctcggg acgattaaca cccaaccagt ttgtggcttt
     6841 gaactgcacc aatcctgcca ggatcttcgg tctttatcct cagaaagggg ctttggtgcc
     6901 aggcgccgat gcagatcttg tgatttggga tcccgaacgc cgcctgacct acgggacagc
     6961 ttacgcccaa catcgcacag attacaacct gtatgagggt tgggaactgg taggctaccc
     7021 cgaaaaagtt ttcttacgcg gcaatttgat cgtcatcgac ggagaatggc gcgggcagcc
     7081 tggcatgggg cgttatttac accggcaggg agccactgta ctgtagtggg cgaatctggc
     7141 agccaacctt ctagccaatt aacgcctgcg tcacgtcatc tcagccggca ggctgtgcgc
     7201 ctatcctgcc cctcccggat tggtttattt ctggcaatca cattgctatc cgcctgccga
     7261 cctcaaacac caacacctgc ccccgcaagc cgcccgccgc tgatttatcc caccgaa
//
LOCUS       DATMXK010000231         3812 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_23338, whole genome shotgun sequence.
ACCESSION   DATMXK010000231 DATMXK010000000
VERSION     DATMXK010000231.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3812)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3812)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3812
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_23338"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..254)
                     /locus_tag="VN363_04310"
     CDS             complement(<1..254)
                     /locus_tag="VN363_04310"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83764.1"
                     /translation="MKNSWQLRISSLLQKQSDQADRPQRISLIGIGYELGGDDAAGVV
                     VIQRLQKSLPAREPFQLIEAGTAPENVTGAVRRFQPDQIVL"
     gene            complement(202..621)
                     /locus_tag="VN363_04315"
     CDS             complement(202..621)
                     /locus_tag="VN363_04315"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWQ83765.1"
                     /translation="MSVVGMLKDVVGSLFKAPFTEQYPFVRNEAPERLRGKLIWSPEK
                     CTGCGLCTKDCPANALEVIIVDRAKKEFIIHYNTSKCTYCGQCVESCRQGSYVMSNVE
                     WELAALTKESFQVTYARPEVLEKFLAAQNQLPAAEAV"
     gene            complement(638..1543)
                     /locus_tag="VN363_04320"
     CDS             complement(638..1543)
                     /locus_tag="VN363_04320"
                     /inference="COORDINATES: protein motif:HMM:NF012373.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="complex I subunit 1 family protein"
                     /protein_id="HWQ83766.1"
                     /translation="MDFVRSLTNILLFPSGLFLMFSGLIYEWVERKLIARMQNRVGPR
                     WFQPIADVFKLLAKEEIVPIGVNELLFNLLPVLGLAGALTAALYVPMFGFEPAFSFSG
                     DLIVALYLLSLVTLCIGLAGSNTQDRFSMVGAVRALTQVFSYEAPFMLALLGPAILAQ
                     SWQIDQIEAFISGRWLLLAQPLGFLVAVIGLMGKLELPPFDAPEAETEIVAGAFTEYS
                     GRGFAIFKLAKSVEMVVGLTLIAAFFMGGLANPLDYLLKTLVLLLILAIISAVLTRFR
                     IDQVVRWWRYGAMLALLQLLLMIVF"
     gene            complement(1649..3319)
                     /locus_tag="VN363_04325"
     CDS             complement(1649..3319)
                     /locus_tag="VN363_04325"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit C"
                     /protein_id="HWQ83767.1"
                     /translation="MDTELALQNASTLLTGLVESSSNPYPNRVDVHLTPDQLLAASTM
                     LIHNHWGYLSAITGIDLGVENGNIEVLYHFCSGAAIVTLRVLTPRLSARVPSLTHLIP
                     STRLYELELREMLGVEIVGLDNPDHLFLPDDWPVGIYPLREDFDPASVPAPGAVPLEI
                     SESKQVGNKFIVPIGPQHPALKEPGHFEFSVDGETITGASIRLGYVHRGIEKACQERT
                     WVQNLYLFERICGICSHTHAYAYSLGVEQLAGVEVPLRAQVIRELVAGLERIHSHLLW
                     VGVSAHEAGFDTLFMYSWRDRETVMDLLESLTGNRVNYSANVLGGVKFDVTSELAKKI
                     KEGMAFLDKRLQTYLDVVSHDAAFLQRTRGVGITPREAAEVLGIVGPTARASGLTRDI
                     RIEAPYGSYTRFPISVVTEQAGDLEAKFIVRIKEMLISSKTIAAIVDNLPPGELTTRM
                     PRKMPAGEAISRIEAPRGEVFYYLKSEGGDRPARIKVRTPSICNFSSVSFLTIGSQLA
                     DVPLIMAGIDPCFSCNDRMVILRRPGQEPQTMAWEDLVLYGQKHYHQR"
     gene            complement(3381..>3812)
                     /locus_tag="VN363_04330"
     CDS             complement(3381..>3812)
                     /locus_tag="VN363_04330"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:1990204 - oxidoreductase complex
                     [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_process: GO:0022900 - electron transport chain
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit B family
                     protein"
                     /protein_id="HWQ83768.1"
                     /translation="VMDGLRLWSRLNSPWLIHFNSGSCNGCDIEILATLTPRYDLERF
                     GVKLQGSPRHADVLVCTGPVTRQARERLLRVYEQMPEPKFVVAVGSCGLSGGVFEGCY
                     NVVGQIDEVLPVNAYIPGCPPRPEAIIDGVVKLLGSLKPAA"
BASE COUNT          882 a         1119 c         1066 g          745 t
ORIGIN      
        1 aggacgatct ggtcaggctg gaatcgacgc accgcgccgg tgacattctc gggcgcggtg
       61 ccagcctcaa tcagttggaa tggttcccgg gcaggcaggg atttctgcag gcgctggatg
      121 accacaacgc cggcggcatc atcgccgccc agctcgtacc cgatcccgat caggctgata
      181 cgctgcgggc gatcggcctg atcagactgc ttctgcagca gggagctgat tctgagctgc
      241 caggaatttt tcaagaactt cgggccgggc ataagtgacc tggaaagact ctttggtcag
      301 ggcagctaat tcccactcaa cattcgacat gacatagctt ccctgacggc agctttcaac
      361 acactgcccg cagtaagtac atttggaagt attgtaatgg ataatgaatt ccttcttcgc
      421 ccggtcgaca ataatgactt ccagggcatt ggccgggcag tctttggtgc acagaccgca
      481 gccggtgcat ttttcaggac tccagattaa cttcccacgc aggcgctcag gagcttcatt
      541 acgcacaaag ggatattgtt ccgtaaatgg ggctttgaaa agcgagccaa ccacatcttt
      601 taacattcca acgacactca tcccaaacct cctctgacta gaaaacgatc attaacaaca
      661 attggagcag cgccagcatg gcgccgtacc gccaccaacg cactacctgg tcaatgcgga
      721 agcgggtcaa taccgctgag ataattgcca ggattaatag cagcaccagg gttttaagca
      781 gatagtccag ggggtttgcc aggcctccca taaaaaatgc ggctatcaaa gtcaatccaa
      841 cgaccatttc gacggatttc gccagcttga agatagcaaa accacgtccg ctgtactcgg
      901 tgaaagcccc ggcgacgatt tcagtctcgg cctcaggtgc atcgaatgga ggcagctcca
      961 gctttcccat caggccgatc acagccacca gaaatcccag gggctgagcg agcagcagcc
     1021 agcgcccgct gataaaagct tcgatctggt cgatctgcca gctctgggcg aggatcgccg
     1081 gacccagcag cgccagcatg aatggcgcct cgtaagagaa tacctgggta agggcgcgga
     1141 ccgcaccaac cattgaaaag cggtcctgcg tgttggaccc ggccagaccg atgcacaggg
     1201 tcaccaggct gagcaggtac agcgccacga tcaggtcacc cgagaagctg aaggctggtt
     1261 cgaagccgaa cattggcacg tacagggctg cggtcagcgc gccggccaga cccaacaccg
     1321 ggagcaggtt gaagagcagc tcgtttaccc ctatggggac gatttcttcc ttcgccagca
     1381 gcttgaacac atcagcgatc ggctggaacc agcgcgggcc aacgcgattt tgcatacggg
     1441 cgatcagctt tcgctctacc cattcataga tcaggcccga aaacatcaga aacaggccgc
     1501 tcgggaaaag aagtatattg gtcaggctgc gtacgaagtc cataggcttt tgttttccaa
     1561 atatctatcg gccatgcctg cgccggctca cccgagcctg gcgcaggata gatctttcct
     1621 gcagttgaat aactggctag aagccgggtc agcgctggtg ataatgtttt tgcccgtaga
     1681 gcaccagatc ttcccaggcc atggtttgcg gttcctgccc cgggcggcgc aggataacca
     1741 tccggtcgtt gcaagagaag cacgggtcaa tccccgccat gatcagggga acatcggcca
     1801 gctgcgaacc gatcgtgaga aaggagaccg aagagaagtt gcagatactc ggcgtgcgca
     1861 ctttgatccg ggccggtcta tcgccgcctt cactcttgag atagtaaaat acctcgcctc
     1921 gcggcgcttc gatccggctg atcgcctctc cggcgggcat tttgcgcggc atgcgggtgg
     1981 tcagctcgcc agggggcaga ttgtcgacga tagcagctat ggttttactg ctgatcagca
     2041 tttctttgat gcggacaata aatttagctt ccaggtcgcc ggcttgctcg gtgaccacac
     2101 tgatcgggaa gcgggtatag gagccgtagg gagcttcgat gcggatatcc cgggtcaggc
     2161 cagaggcgcg ggctgtgggg ccaacgatgc ccagaacttc ggcagcctcg cgcggcgtaa
     2221 tccccacacc gcgcgtgcgc tgcaggaatg ccgcatcgtg gctgacgaca tccagataag
     2281 tctgcaggcg cttatcgagg aatgccattc cctctttaat tttcttcgcc agctcgctgg
     2341 taacgtcgaa cttcaccccg cccaggacat tggccgagta atttacccgg ttgccggtca
     2401 ggctttccaa caaatccatc acggtttccc ggtcgcgcca ggaatacatg aacagggtat
     2461 cgaaacccgc ttcgtgagcc gagacaccca cccacagcag gtggctgtga atccgctcca
     2521 gacccgcaac cagctcccgg atcacctgcg cccgcagggg tacttcaacg ccagccagct
     2581 gctccacacc cagggagtag gcataggcgt gggtatgcga acaaatcccg caaatccgct
     2641 caaaaagata caggttctga acccaggtgc gctcctggca ggccttctcg atgccgcgat
     2701 gcacgtagcc caggcgaatg gaggcgccgg tgattgtttc accatcgact gaaaattcaa
     2761 aatgacccgg ctctttcaag gcagggtgct gcggcccgat ggggacaata aatttgttcc
     2821 caacctgctt gctttcgcta atctccaatg gaacggcacc cggagctggt acactggcag
     2881 gatcgaaatc ctctcgcagc gggtagatac cgaccggcca atcgtccggc aggaagagat
     2941 ggtcggggtt atccaggccg acaatctcga caccgagcat ttctcgcagc tccagctcat
     3001 acaggcgagt cgaggggatt aaatgggtca ggctgggcac ccgcgcactc aggcggggag
     3061 taagcacccg caaggtgacg atcgcagccc cggagcagaa atggtacagg acttcgatgt
     3121 tcccgttttc cacacccaga tcgatcccgg tgatggctga gagatatccc cagtgattgt
     3181 ggatcaacat cgtcgaggct gccagaagct ggtctggagt taggtgcacg tccacgcgat
     3241 tgggatacgg attgctgctg ctctccacca acccggtcag cagagttgaa gcattctgta
     3301 atgcgagctc agtatccata aacaatctct ccctcttcaa acaaagcgaa atacaaataa
     3361 attcatgccc aagcagcctt tcaggcggcc ggttttaagc tgcccagcag cttgacaacg
     3421 ccatcgatga tggcttcagg ccggggtggg cagcccggga tataggcgtt taccgggaga
     3481 acttcgtcaa tctgcccgac cacattgtag cagccctcaa aaacgccacc cgacaggccg
     3541 cacgagccga ctgcaaccac gaatttaggc tcgggcatct gctcgtaaac gcgcagcaag
     3601 cgctcccgcg cctggcgagt caccggaccg gtacagacga gcacatccgc gtgccggggg
     3661 ctgccctgca gcttgacccc aaacctttca aggtcgtagc ggggggtcag ggttgccagg
     3721 atctcgatgt cgcagccatt gcacgatccg ctgttgaaat ggatcagcca gggagagttc
     3781 aagcgcgacc agagcctgag cccgtccatc ac
//
LOCUS       DATMXK010000232         2062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_75488, whole genome shotgun sequence.
ACCESSION   DATMXK010000232 DATMXK010000000
VERSION     DATMXK010000232.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75488"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(169..579)
                     /locus_tag="VN363_04335"
     CDS             complement(169..579)
                     /locus_tag="VN363_04335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83769.1"
                     /translation="MNNSNGILVDLKRCTGCDACTLACQQEHFLSPGNSFIRVQTVGG
                     PGVDEPSGKFPDLHLYWRPLIQKGCNLCQERTSAGFETACVENCYTDALTYGNLSDPH
                     SVISLKIAELTSAGRVIHQAEPFLATRENIYYAY"
     gene            complement(590..>2062)
                     /locus_tag="VN363_04340"
     CDS             complement(590..>2062)
                     /locus_tag="VN363_04340"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ83770.1"
                     /translation="EKAGQAKTVAPGAYGLDFKPALTGSYQVAGVACKPAFLLLKEHA
                     SLYPPEKTEAITGVPAETVRRLAREMASIKPMYIATNMGMRYWEAYQAYRGMNLLAAL
                     TGNYGLRGGGRYLPSAIAAPIFNVPDVTNPTDSSVSSLRWDMAMKAMETGEPYPIKGF
                     FVIGNNPLNCTPNRNAWLKQRFPNLDLIVVNDIFMTDTAQYADYVLPDASVFERNDID
                     FQYGCVVLQQKAVEPPQGVLNRVQMFSELARRLGFGEYFDKTDEEWVEVMLQSEHPFV
                     KGITVAELKSNGGMVAARVPLGGMKAFPEGKFPAAASGRLEFYSEYLAPSGDALFQYK
                     PSLESSHARDGKEYPLTFLSRRKRFRTQSLFANDPALLTIESAPALDINPLDAEARNI
                     HHGDLSHVFNSRGEMYAKARLTENVPQGMVNFDHGWLSSQQEGGHYQELILDVGSDAT
                     YSAAHEVWWNRIMANQIWWEVFFAGRPDILWDCAVQLEKA"
BASE COUNT          415 a          588 c          603 g          456 t
ORIGIN      
        1 cggccgggtg tgctcccacg cttgcatctg aaagacgcgg tagcctttgt cacgcagctc
       61 agccatgcgc gtggagatgg ggctctgggc gtcgtccagc cggggtgaag tgggggatga
      121 aggcattgga cccctccccc acttcactat ctttggtctg cctgtcactt aataggcgta
      181 gtagatattt tctcgcgttg ctaagaacgg ctcggcctgg tgaattaccc gcccagcact
      241 agtcagctcg gctatcttga gcgagatgac gctgtgtggg tcggacaggt tgccgtaggt
      301 caaggcatcc gtgtagcagt tttccacgca ggccgtttca aaaccggcgc tagtgcgctc
      361 ttggcacagg ttacagcctt tctgaatcag tggccgccag tagaggtgca gatcaggaaa
      421 cttacctgaa ggttcgtcaa ctcctggacc gcccacagtt tgaacgcgaa tgaatgagtt
      481 acccggtgag aggaaatgct cctgctggca agccagggtg caagcatcgc aaccggtgca
      541 gcgcttgaga tctaccaaaa tcccgttcga gttattcatc agacacctcc taagcctttt
      601 ccagctgaac ggcgcaatcc catagaatgt cggggcgacc agcgaagaac acttcccacc
      661 agatctggtt cgccataatc cggttccacc aaacttcgtg ggctgcgcta taggtggcgt
      721 cgctgccaac gtccaggatc aactcctgat agtgaccgcc ctcttgctga gaactcaacc
      781 agccgtggtc gaagttgacc attccctggg gaacgttctc ggtcagccgg gccttggcgt
      841 acatctcgcc gcgactattg aacacgtgac tcagatcacc atgatgaatg ttgcgcgctt
      901 cagcatccag cgggttgatg tccagcgccg gtgcggattc aatggtcagc agggcagggt
      961 cgttagcgaa cagcgactgg gttcggaagc gcttgcggcg gctcaggaag gtgagcggat
     1021 attctttccc gtcccgggca tgagagctct ccagcgaggg tttatattgg aacagggcat
     1081 cgccgctggg cgccaggtat tcgctgtaga actccaaccg gcctgaagct gcggcaggga
     1141 acttcccttc cgggaaagct ttcatcccgc ccagcgggac gcgagctgcc accatacctc
     1201 cgttgctttt tagctcagcc accgtaatgc ccttcacgaa gggatgctct gactggagca
     1261 tgacctcgac ccactcctca tcagtcttgt cgaagtactc gccaaacccc aggcgtctgg
     1321 ccagctcgct aaacatctgg acccggttga gcactccttg cggcggttcg actgcttttt
     1381 gctggagaac gacacagcca tactggaagt caatatcgtt gcgttcgaat acactcgcat
     1441 caggcagcac gtagtccgca tactgggctg tatcggtcat gaaaatatca ttgactacga
     1501 tcaggtccag gttgggaaaa cgctgtttga gccaggcgtt ccggttgggg gtgcagttga
     1561 gcgggttgtt gccgatgaca aagaaacctt tgatcgggta gggttctccg gtctccatcg
     1621 ccttcatggc catgtcccag cgcagggagg agaccgaaga gtcggttggg ttggtgacgt
     1681 cgggtacatt aaagatgggg gcagcgattg ccgatggcag gtagcgaccg cctccgcgta
     1741 agccgtagtt cccggtgagc gccgccagca ggttcatccc ccggtaggcc tggtaggcct
     1801 cccagtagcg catccccatg ttggtggcaa tgtacatcgg tttgatggaa gccatctcgc
     1861 gcgccaggcg gcgcactgtt tcagccggaa caccggtgat ggcctcagtt ttttccgggg
     1921 gatacaggct ggcgtgctct ttaagcagca ggaaagccgg tttgcatgcc actccggcaa
     1981 cctgatagct gccggtcaaa gccggtttaa agtccagccc ataagctccc ggcgctacag
     2041 tcttcgcctg cccggctttc tc
//
LOCUS       DATMXK010000233         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_137111, whole genome shotgun sequence.
ACCESSION   DATMXK010000233 DATMXK010000000
VERSION     DATMXK010000233.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137111"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2237)
                     /locus_tag="VN363_04345"
     CDS             complement(<1..>2237)
                     /locus_tag="VN363_04345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83771.1"
                     /translation="DQLDGKGWAVMTNGKLWRLYSARAHSRATNYYEIDLEETLASPE
                     PGVAFRYFWLFFRSAAFDLTQSHPQPLPSEKTSVDRGARFLDRMLDESASYAKALGER
                     LKSRVFDEVFPHFAEGFIQHWRKTDGKLDFSDGELGQVFQATLTFLYRLLFLLYAEAR
                     DLLPLREVRDYHDVSLLSIIETVAQKAGPVKDEAKEKLIKAYALDSTGLYDALTRLFK
                     TVDRGDATLNVPVYNGGLFITFEGRLPQPGEEDSAELHNAVFLTEHKIPDRFLALGLD
                     RLARDVDDRTFALAMIDYKSLGVRQLGSIYEGLLEFKVRIAGEKMAVIRGKKGDEVVP
                     YRQAMKDGLKIKTSGRGSARTEETLPKGAVYLTNDRRERKATGSYYTPDYIVKYIVQH
                     TVGPALEAKCEEVRPLIRDAQQAYHRAIDRQKAFQKQGMQGDDPEKVAHSYQHVVDAL
                     FDLRVLDPAMGSGHFLVEAVDFITDRMLTFLSAFPWNPVTAALRQTRQTILEEMQRQG
                     VNVDPARLTDVHLLKRQVLKRCIYGVDLNPMAVELAKVSLWLDCFTLGAPLSFLDHHL
                     KCGNSLIGARIEEVRAALDMAAEKDRLEIPLLAGMMGSQFAGVMLATDLMRRVGELSD
                     VTAAQVRESRDEYRRASDALAPYRRILDLYTSRWFGNPDTKLSSPVLGFLRNSKAEAW
                     LKNPSERYMKLKPDEYKLGEIALRVAREKRFFHWELEFPEVFFGPSKSSAQKIEMKEN
                     PGFDA"
BASE COUNT          444 a          662 c          626 g          505 t
ORIGIN      
        1 cggcgtcaaa gcccggattt tccttcatct caattttttg ggcgctggat ttgcttgggc
       61 cgaagaaaac ctctgggaat tccagctccc aatgaaagaa gcgcttttcg cgcgccactc
      121 tcagtgctat ctctcccagc ttatactcgt ctggcttcag cttcatgtat ctttctgagg
      181 gatttttaag ccaggcttcg gctttcgaat tgcgcaagaa acccagcacg gggctgctca
      241 gctttgtatc cgggttacca aaccagcggc tggtgtagag atccaggata cgccggtaag
      301 gcgccagggc gtcgctggct ctgcggtatt cgtcgcggct ttcgcgcacc tgggcggcgg
      361 tcacgtctga aagctcgccc acacggcgca tcaggtcggt ggccagcatc accccggcaa
      421 actggctgcc catcatcccc gccagcaggg ggatctccag gcggtccttc tcggcggcca
      481 tgtccagggc ggcgcgtact tcctcgatgc gcgccccgat caggctgttg ccacacttca
      541 ggtgatgatc aaggaaggac agcggtgcac ccagggtgaa acaatccaac cacaggctga
      601 ctttggccag ctccactgcc atcgggttga ggtccacgcc gtagatgcag cgcttgagca
      661 cctgcctttt gagcaggtgc acgtccgtca atcgcgccgg gtcaacgttc accccctggc
      721 gctgcatttc ttccaggatg gtctggcgcg tctggcgcag ggcagccgtc accgggttcc
      781 aggggaaagc actcaggaaa gtcagcatcc ggtcagtgat gaaatccacc gcttcaacga
      841 ggaagtgccc gctacccatg gccgggtcca gcacgcgcag gtcgaacaga gcgtccacca
      901 catgctggta gctgtgagcg actttttccg ggtcgtcacc ctgcataccc tgcttctgga
      961 atgccttctg ccgatcgatt gcccggtggt aggcctgttg agcatcgcgg atcaatggac
     1021 gtacctcttc acatttggct tccagagccg gaccgacggt gtgctggacg atgtatttga
     1081 cgatgtaatc gggcgtgtaa tagctgccgg tggctttgcg ctcgcgccgg tcgttggtca
     1141 ggtataccgc acctttgggc agcgtctcct cagtgcgggc tgagccgcgc ccgctggtct
     1201 tgatcttcag gccatccttc atcgcctggc ggtaaggaac gacttcatca cccttcttgc
     1261 cgcggatcac agccattttc tcgccggcga tgcgcacctt gaattccagc agcccttcat
     1321 agatcgagcc aagctggcgc actcccagcg atttgtaatc gatcattgcc agggcaaagg
     1381 tcctgtcatc cacatcgcgc gccagacgat ccaatcccag cgccagaaaa cgatcgggga
     1441 tcttatgctc tgtcaggaag accgcattat gcagctctgc agagtcctct tcacccggtt
     1501 gaggtaatct accttcgaag gttataaata atccgccatt gtagactggc acgttcagcg
     1561 tggcgtcccc gcgatcgacc gttttgaaca ggcgtgtcag cgcatcgtac aatccagtcg
     1621 agtcgagtgc atatgctttg atcagttttt ctttggcctc atcctttacc ggaccggcct
     1681 tttgagctac ggtttctatg atgcttagca gtgatacatc gtggtagtcg cgcacctcgc
     1741 gcaggggtaa tagatccctc gcttcagcat acagcaggaa aagcaggcga tacaggaatg
     1801 tcagggttgc ctggaagacc tggcccagct cgccatcgct gaaatccagc ttgccgtcag
     1861 ttttacgcca gtgctgaata aaaccctctg caaagtgagg gaagacttcg tcgaagacgc
     1921 ggctcttcag gcgttccccc aacgcctttg cataactggc tgattcatcc agcatgcggt
     1981 cgaggaaacg ggccccccta tccactgatg ttttttcgga ggggaggggc tggggatggg
     2041 actgagtcag gtcaaatgct gccgaccgga agaataacca gaaatagcgg aaagccactc
     2101 ctggctcggg tgaagccagg gtttcctcga ggtcaatttc ataataattg gtggcgcgcg
     2161 agtgagctcg agcagagtac aggcgccaga gcttcccatt ggtcatcact gcccagccct
     2221 taccgtccag ctgatcc
//
LOCUS       DATMXK010000234         5865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_102465, whole genome shotgun sequence.
ACCESSION   DATMXK010000234 DATMXK010000000
VERSION     DATMXK010000234.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_102465"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1267)
                     /locus_tag="VN363_04350"
     CDS             complement(<1..1267)
                     /locus_tag="VN363_04350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83772.1"
                     /translation="MTTTAESTLKASPAAHNSLAKNIALFLGWVLFTALFSLAYTQAP
                     LYEGNQNTKFLHGLAEAGRGYLQYDWLANTVDPLPVFSALVNITARISESLFYYYYAV
                     LFGIYIFSLYGIADFIFELRKSWAKSLLFLGLFFTAHSAWLLFEMEKQHGLDMTFLHF
                     GLAGQYLLGLEFQNSSFGVLLLLSIYLFLNNKHTWAILALGTACLFHPAYLFSAALIV
                     LAYLGIVFFKTLPPSANTSRFNFPRILQAARKPFLLGCLALILVLPVIVYNQVFLGAT
                     SPEISAQAMDILVHYRIPHHSLPAVWFNNFSLIQIGMMLVGLLLARKSRLFGIMASLA
                     GGGLLLSGIQILTQSNDLAYLAPWRVSVLLVPVSLALILAYLVSILVDHLPLPKILTF
                     SLAVVLGVFLIYKSVDIGQERSATYRTSAR"
     gene            complement(1275..2150)
                     /locus_tag="VN363_04355"
     CDS             complement(1275..2150)
                     /locus_tag="VN363_04355"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family protein"
                     /protein_id="HWQ83773.1"
                     /translation="MANDPTPIPARPHTVAIIQARMGSSRLPGKVLLDIAGRPMLGWV
                     IERTRRASLVDEVVLATTTDPSDDPVADYCQSNGYAYTRGSLGDVLDRYYQAAQAHQA
                     EVIARVTADCPLIDPTLIDDVIGVFLGAPPRIASRLEIREAENPESRLAPYPFDFAAN
                     RLPPPFRRSYPVGLDVEVCSFTALERAWLEARQGFEREHVMPYFYSQPGRFRFVQLQH
                     AQDFGSLRWTVDTAQDLTLIQEIFNRLPDPLTCSWLDVLQIIQSDPSLSNLNAQVKAK
                     SYQDVDSRLKPEDLV"
     gene            complement(2150..3286)
                     /locus_tag="VN363_04360"
     CDS             complement(2150..3286)
                     /locus_tag="VN363_04360"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ83774.1"
                     /translation="MARILYFTRDFTTHDHRFLAALAETEHQVYYLQLERRGHTLEDR
                     PLPDGITRVEWAGGNQPFTWDQIKPLTIDLQRVIAEVRPDLIQAGPVQTAAYLVALTG
                     FTPLVTVSWGSDLLVDARRSARYRRVTRYTLAHSAALVGDCQPVHAAAVGEGMPPDKI
                     ITFPWGVDLVHFSPTGPALEPALFAGQSGPKFVILSTRAWEPIYGVDILAQGFVLAAR
                     EIPELRLVMLGYGSLAGKLHSIFASAQMLERVIFPGQISQADLPKYYRSADLYSSASH
                     SDGSSISLLEAMACGRPVLVSDIPGNRAWVTPGVQGWWFADGNPRSLAEGLGLAFAYR
                     QDLHEIGQAARRLAEARANWPDNFKKLQLAYDLALSNSAIASKN"
     gene            complement(3297..4367)
                     /locus_tag="VN363_04365"
     CDS             complement(3297..4367)
                     /locus_tag="VN363_04365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559065.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HWQ83775.1"
                     /translation="MNIQIGRHTIGRDYPTYFIADISANHDGSLERALLLIRLAKEAG
                     ADAAKFQNFRASQIVSDYGFRNMGGQLSHQANWKESVFEVYQAASLPFEWTPILKEEC
                     DRVGIDYFSSPYDFEAVDMLDPYVPAHKIGSGDITWPEMLEHIARTGKPVILSTGASD
                     IGEVQRAVHTILKINPQLVVLQCNTNYTASLENFDSIHLRVLDTYRVMFPDIVLGLSD
                     HTAGHATVLGAVALGGRVVEKHFTDDITRTGPDHPFSMTPQTWAEMVQRTRELERALG
                     SADKVVAENEKQTVIVQRRCLRAARPIQAGEVLTREMIDVLRPAAPGAILPDEIGAVI
                     GAKAAVDLPSGRELRWTDLINA"
     gene            complement(4364..5275)
                     /locus_tag="VN363_04370"
     CDS             complement(4364..5275)
                     /locus_tag="VN363_04370"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ83776.1"
                     /translation="MRVLVTGASGLLGANLALEAAKDHTVYGLVNTLDLHTEAFQVLK
                     GDLAEPGEIERLLDITQPEWVIHCAALANVDACETDQARAVQLNTEVPRKLAINVARG
                     GARLLHVSTDAVFDGIRGNYVESDPPNPLSVYARTKLDGERAVAEANPDAMIARVNLF
                     GWSPSGRRSLAEWFFNNLSTGKPVMGFTDVYFCPLLANDLGKIFLDMLQNQLRGLYHV
                     VARDCQSKYEFGVQLARLFNLDAGLIQPASVMASGLAAARSPNLTLNTAKLTRALGYP
                     PPDIQQGLQSFYQLHQQNYPHRLREML"
     gene            complement(5353..>5865)
                     /locus_tag="VN363_04375"
     CDS             complement(5353..>5865)
                     /locus_tag="VN363_04375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559063.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis protein"
                     /protein_id="HWQ83777.1"
                     /translation="RYGNVVGSRGSVVPIFLLQRNNGKLTITDERMTRFWLTLEQGAR
                     FVIRCVEQMHGGEVFVPKIPSTHIVDLARAIAPGAEMDVIGIRPGEKLHEVLIHEDEA
                     RSTVEMDDMYVVQPTGSLWFGRDWEKIGKSLPDGFRYSSDNNSHWLNMAEIQAMVAPI
                     ESEISSGSLS"
BASE COUNT         1386 a         1581 c         1655 g         1243 t
ORIGIN      
        1 gtctggcgct ggtgcggtaa gtagcgctgc gctcctggcc aatatcgacg gatttgtaaa
       61 tcagaaaaac tcccagaacc acagccaggc tgaaggtgag tattttgggc agcggcaggt
      121 ggtctactag gatggagacg agatatgcca gaatcaatgc cagcgataca ggaaccaata
      181 aaactgacac tcgccaggga gccagatacg ccagatcatt actctgggtg agaatctgta
      241 tccctgagag cagcaatccg ccgccagcca gggaagccat aatcccaaac agcctggatt
      301 tgcgcgccag gagcagcccg accagcatca ttccaatttg aataagggag aaattattga
      361 accacaccgc agggagtgaa tggtggggga tacggtagtg gaccaggata tccattgcct
      421 gggcggaaat ttctggagag gtcgctccca gaaaaacctg gttataaacg atgaccggga
      481 ggacaagtat caatgccagg cagccaagca ggaatggttt tcgggctgcc tgaaggatcc
      541 ggggaaaatt aaaccggctg gtgtttgcgc tgggaggtaa ggtcttaaaa aagacaattc
      601 caagatatgc gagtacgatc agagccgcgc taaataaata ggccggatgg aataaacagg
      661 cagtgcctaa ggcaaggatc gcccaggtat gcttgttatt gagaaacagg tagattgaaa
      721 gtagaagcag gacgccaaat gagctgtttt gaaactcgag gcctaacaaa tactgaccgg
      781 ctaatccgaa atgcaggaaa gtcatatcca ggccgtgctg tttttccatt tcgaacaaca
      841 gccaggctga atgtgcggta aagaatagac ccaggaagag gagtgatttt gcccagcttt
      901 tgcgcagctc aaagataaaa tcggcgattc catacaggct gaagatatag atcccgaaca
      961 aaaccgcgta atagtaatag aacaggcttt cactgatccg ggcggtaata tttaccaggg
     1021 cagagaaaac tggcagtgga tcgaccgtgt tcgccagcca atcgtactgt aagtaacccc
     1081 gcccggcctc agccagacca tgcaggaatt tggtattctg gttgccttca taaagcggcg
     1141 cctgggtgta agccaggctg aagagggccg tgaaaagtac ccaccccagg aagagggcga
     1201 tatttttagc cagcgaatta tgagctgctg gactggcttt cagggtggat tcagcagtgg
     1261 tagtcatatt ttcgttacac aaggtcttcc ggtttcaggc gcgagtcgac gtcctgataa
     1321 cttttggctt tcacctgagc gttgaggtta cttaaagatg gatcgctctg aataatctgc
     1381 agcacatcca gccaggagca ggtgagcgga tctggcaggc gattaaaaat ttcctggatc
     1441 aaggtcagat cctgggcagt gtcaaccgtc cagcgcaggg agccgaaatc ctgcgcatgc
     1501 tggagctgca caaaccgaaa tcgccccggt tgagaataaa aatacggcat gacgtgctca
     1561 cgctcaaagc cctgcctggc ttcgagccag gcgcgttcca gggcagtaaa tgagcagact
     1621 tccacatcca ggccgactgg atagctgcgc ctgaaaggcg gtggcaggcg gttggcagca
     1681 aagtcgaatg gatagggcgc cagccgggac tctggattct cagcttcgcg gatttccagg
     1741 cggctggcga tccggggagg cgcacccaga aaaacgccaa tcacatcatc gatcagggtt
     1801 ggatcgatca gcgggcagtc agcggtcacg cgcgcgatta cttcagcctg gtgagcttgc
     1861 gccgcctggt agtaacggtc cagcacatcc cccaggctgc cgcgggtgta agcataacca
     1921 ttgctctggc agtaatccgc caccggatcg tcgctcggat cggttgtcgt ggccaggacg
     1981 acttcatcca caagactggc gcggcgagta cgctcaataa cccagcctaa catgggacgt
     2041 ccggcgatat ccaggagcac ttttcctggc aggcggctgg aacccatgcg ggcttgaata
     2101 atggctacag tatgaggccg ggcggggata ggtgtgggat cgtttgccat taattcttag
     2161 aggctatcgc tgaattactg agcgccagat catatgccag ctggagcttt ttgaaattat
     2221 ctggccagtt ggcccgcgct tcagcaaggc gccgggcggc ctgtccgatc tcatgcaggt
     2281 cctgccggta tgcaaaagcc agacccagcc cttctgccag agaacgagga ttgccatctg
     2341 caaaccacca gccctgcaca ccaggcgtta cccaggctcg attgcctgga atatcggata
     2401 ccagtacagg acgcccacaa gccattgctt cgagcaagga gatcgaactt ccgtcgctgt
     2461 gcgatgcgct ggaataaagg tcagcgctgc ggtagtattt gggtaaatct gcctgcgaaa
     2521 tttggccggg gaagattact cgctcaagca tctgcgcgct tgcgaagata ctgtgtagtt
     2581 tgccggcgag tgatccatag ccaagcataa ccaggcgcag ctcggggatt tccctggctg
     2641 ccagaacaaa tccttgcgca aggatgtcca caccgtaaat tggctcccag gcgcgtgtag
     2701 aaaggatgac aaactttggt ccagattggc cggcgaagag tgctggctcc agggcaggcc
     2761 cggtgggaga aaaatggacc agatccaccc cccaagggaa agtgataatt ttatccgggg
     2821 gcatcccctc gccaaccgcg gccgcgtgga ctggctggca gtcgcctacc aatgccgcac
     2881 tgtgtgccag ggtatagcgt gttactcgcc ggtaacgagc actgcgtctg gcatcgacca
     2941 acagatcgga accccatgaa acggtcacca gcggtgtaaa gccagtcagt gcaaccagat
     3001 aggctgcagt ttggaccggc ccagcctgga tcagatcggg ccgcacttca gcgatcacac
     3061 gctggagatc gattgtcagg ggttttatct gatcccacgt aaaaggttga tttccacctg
     3121 cccactcgac cctggtaatt ccatccggta aaggccgatc ttccagggtg tgcccacgcc
     3181 gctccaactg cagatagtaa acctgatgct ctgtctcagc cagagcggcc agaaagcggt
     3241 gatcgtgggt cgtgaagtcg cgggtgaagt acagaatgcg agccactcag ctccactcag
     3301 gcattgatca aatctgtcca gcgcagctca cgcccggagg gaagatcaac tgccgccttc
     3361 gcgccgatca ccgcaccaat ctcatccggt aagatcgccc cgggtgctgc ggggcggagt
     3421 acatcaatca tctcccgtgt gaggacctca cctgcctgga ttgggcgagc agccctcagg
     3481 cagcgccgtt gaacgatcac ggtttgtttt tcgttttcag cgacaacctt atcagccgag
     3541 ccaagagccc tctccagctc gcgcgtccgc tggaccattt cagcccaggt ctggggtgtc
     3601 atggagaatg gatgatccgg gccggtgcgg gttatatcgt cggtgaagtg tttctcgacg
     3661 acgcgccctc ccagggcgac ggctcccaga acagtcgcgt gcccggcagt gtggtctgat
     3721 aatcccaaaa ctatatcggg gaacatcacc cggtaggtat ccaaaacgcg taaatggatg
     3781 ctgtcaaaat tctcaaggct ggcggtatag ttcgtattac actgcaagac gaccagctgg
     3841 ggatttatct taaggatggt atgaacggcg cgctgtacct ccccgatatc cgacgccccg
     3901 gtggataaaa taaccggctt gcccgtgcga gcgatatgct ccagcatctc gggccaggtg
     3961 atatccccgg agccgatctt gtgagcgggt acgtagggat cgagcatatc tacagcttcg
     4021 aaatcgtagg gcgatgaaaa ataatcgatc ccaacccggt cacattcttc tttgagtatg
     4081 ggtgtccatt cgaaagggag cgaggctgct tgataaactt caaagaccga ttccttccaa
     4141 tttgcctggt gggagagctg tccgcccata ttcctgaaac cataatcaga gacgatctgg
     4201 gaggcgcgga agttttggaa cttggctgca tctgctccgg cttcctttgc caggcggata
     4261 agcagcaggg cgcgctccag gctgccatcg tggtttgcgg aaatatcggc gatgaaatag
     4321 gtcggatagt cgcggccaat cgtatgcctg ccgatctgga tattcataac atctccctga
     4381 gtctgtgtgg ataattttgt tggtgaagct ggtagaagct ttgcaaacct tgctggatat
     4441 ctggcggcgg gtatcccagc gcccgggtta atttagcggt attcaaggtc agattgggcg
     4501 agcgggcagc cgccaacccg gatgccatta ctgaggcagg ctggatcagg ccggcgtcta
     4561 aattaaagag cctggcgagc tggacaccga attcgtattt gctctggcag tccctggcaa
     4621 cgacgtggta caggccgcgg agctggttct gaagcatatc cagaaaaatt tttcccaggt
     4681 cattggccaa taaggggcag aagtatacat ccgtaaaacc catgaccggt ttgcctgtgc
     4741 tcaggttatt gaaaaaccac tctgccaggc tgcgcctgcc cgaggggctc cacccgaaaa
     4801 ggttaacccg cgcaatcatc gcatctggat tggcttcagc cactgcacgc tccccatcca
     4861 gcttggtacg ggcgtaaacg ctcagtgggt ttggcggatc actttccaca tagttcccgc
     4921 ggatgccgtc gaagacagca tcagtggata catggagcag gcgcgctccg cccctggcga
     4981 cattaatcgc cagtttacgt ggaacctcag tattcagctg caccgcccgg gcctggtctg
     5041 tctcgcaggc atcgacattc gccagcgctg cacaatggat cacccattcg ggttgggtga
     5101 tgtccagcag gcgttcgatc tcgcctggct ctgccaggtc gcctttcaac acctgaaaag
     5161 cttccgtgtg cagatcgagc gtattcacca gcccatagac ggtgtggtct ttcgccgcct
     5221 ccagcgccag gtttgcgcca agcaaaccac tggcccctgt gacgaggaca cgcatgacct
     5281 ctccccgcct gacaaacgat ccgttttgtt aatctaccgg gctgacaacc cctatccgcc
     5341 cggtattcgt gctcagctta aactgccgct gctgatctct gactcaattg gggctaccat
     5401 ggcctgaatt tctgccatgt tcaaccaatg cgagttattg tcgctgctgt agcgaaatcc
     5461 gtctggcagg cttttaccaa tcttttccca atctctgccg aaccagagcg atcccgtcgg
     5521 ttggacaacg tacatatcat ccatctcaac ggttgagcga gcttcgtcct catggatgag
     5581 tacttcatgc agtttttcac ccgggcggat accgatcacg tccatctctg ccccaggtgc
     5641 gatagcccgg gccagatcca cgatgtgggt ggaagggatc ttggggacga aaacctcgcc
     5701 gccgtgcatt tgttcgacgc agcggatgac gaaacgcgct ccctgctcta aagtcagcca
     5761 gaagcgggtc atgcgctcat cggtaatcgt aagtttgcca ttattgcgct gcagtaaaaa
     5821 gatcgggacg acgctgccgc ggctgccaac tacattgcca taccg
//
LOCUS       DATMXK010000235         2244 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_65114, whole genome shotgun sequence.
ACCESSION   DATMXK010000235 DATMXK010000000
VERSION     DATMXK010000235.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2244)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2244)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2244
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65114"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(322..1443)
                     /locus_tag="VN363_04380"
     CDS             complement(322..1443)
                     /locus_tag="VN363_04380"
                     /EC_number="2.3.1.180"
                     /inference="COORDINATES: protein motif:HMM:NF006829.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0033818 -
                     beta-ketoacyl-acyl-carrier-protein synthase III activity
                     [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase III"
                     /protein_id="HWQ83778.1"
                     /translation="MSPFAHITGWGTSVPERVLTNAELEKMVDTDDEWIVSRTGIRER
                     RIAGEDDTTASLATEAALKALLIAGLKPSDVDLVIVSTSSPEHVFPATACLVQDRIGA
                     THAGAFDLSAACSGFIFGLHLASQSIRTGAIRNAVVIGSETLSRLVNWQDRGTCILFG
                     DGAGAFVLQAAEEPGGVLQGVIRSDGSGGDLLSVPAGGSRHPISHKMIEAGLQYIQMN
                     GREVFRFATRVMTQATEEVLRKAGVHLDDIQWVIPHQANLRIIEAAVRGLKLPMERCV
                     VNLERYGNTSTASIPLATVEAIRDGRIKPGDKLVFVGFGAGLTWGAVYVEWTGPFAAR
                     RVVIRPVYIRLLARLRSILLRLTRRLDGIIWGRVSHLDE"
     gene            complement(1459..>2244)
                     /locus_tag="VN363_04385"
     CDS             complement(1459..>2244)
                     /locus_tag="VN363_04385"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWQ83779.1"
                     /translation="VTACATGTNAVGEAAELIRRGQADVVLAGGSEAGIVPIAMAGLS
                     VMTALSTRNDEPQRASRPFDLDRDGFVMGEGSAILVLESEDYARARGARILAEVSGYG
                     ATNDAYHISAPAENGAGAARCMRNALEHANLGLDEIQYINAHGTSTQLNDKSETAAIK
                     TVFGEQAYNLPVSSTKSMTGHLLGASGALEALICTLALRDQVMPPTINYETPDPVCDL
                     DYVPNTCRPGKLHHVMSNSFGFGGHNATIILSQPSDSSGVNSD"
BASE COUNT          545 a          613 c          593 g          493 t
ORIGIN      
        1 tgaatttcgg aggcgacttc gttaatcgtc agcacctcct gagcggaagc ttgctggtta
       61 aagttaaaaa cgaccattgc tatgatcgca acaaataaca gcaagtaaat aagagaagat
      121 cgatttcgag ctgggttcac attacctcca aatcggatga caatcgcatg aattgtagaa
      181 attataccaa cctatctggg cttcgcagaa ctggtttatt caagttgtag aatttctaac
      241 atcgttctaa catttggagc gctggtaaaa aaaacacccg ctgtgtaaac cagcaggtgt
      301 tttgatggat ggatttggat ttcattcatc cagatgggat accctccccc aaataatgcc
      361 atcgaggcgg cgagttaacc gtaacaatat tgagcgcaag cgtgcgagca ggcgaatgta
      421 aaccgggcga ataacgacgc gtcgggcagc aaaaggaccc gtccattcga cgtacacagc
      481 tccccaggtc agtccggctc caaagccaac aaacacaagc ttatccccgg gtttaattcg
      541 cccgtcacgg atcgcctcta ctgtagccag aggaatcgac gctgttgacg tattcccata
      601 gcgctccaga tttacgacac agcgctccat cggcagcttc aatccacgga cagcagcttc
      661 gatgatgcgc aggttcgcct gatgggggat gacccattgg atatcatcga gatggacacc
      721 agccttgcga agtacttcct cggttgcctg ggtcatcacc cgggttgcga accggaatac
      781 ctcgcggcca ttcatctgga tgtactgtaa gccagcctcg atcattttat ggctgatggg
      841 gtggcggctt cctcctgctg gcacgctcag cagatcccca ccagagccgt ccgagcgaat
      901 cacaccctgc agcacaccgc caggctcttc ggcagcctgc aggacaaaag cgcctgcacc
      961 atctccaaac aggatacatg tgccgcgatc ctgccagttg accagacgtg aaagcgtttc
     1021 agaaccaata acgactgcat tgcggatcgc gccagtccgg attgattgcg atgccagatg
     1081 taagccaaaa ataaaacctg aacaagctgc ggatagatca aaagcgccgg cgtgggtagc
     1141 cccgattcga tcctggacca ggcaggcagt ggcagggaac acatgctcag gtgaagaagt
     1201 cgaaacgatg accagatcaa catcggatgg tttaagaccc gcgatcagta atgcttttaa
     1261 ggctgcctcg gtagccaaag acgctgttgt atcatcttca cctgcgatcc gccgttcgcg
     1321 gatacccgtc cgggagacga tccattcgtc gtcggtatcc accatttttt ccagctcagc
     1381 attggttaat acccgttcgg gaacggaggt tccccaacct gtgatgtgtg caaaggggct
     1441 catcggccaa ctctccggct agtctgaatt tacaccgctc gagtcgctgg gctggctcaa
     1501 gataattgta gcgttgtgtc caccaaagcc gaatgaattc gacataacgt ggtgaagctt
     1561 tccgggacgg caggtatttg gcacataatc caaatcacat accggatcgg gggtttcata
     1621 gttgatggta ggtggcatca cctgatcacg gagcgcgagg gtacaaatta aggcttccag
     1681 ggctcctgaa gcccccagaa ggtgaccggt catcgatttt gtcgacgata cagggaggtt
     1741 gtaggcctgc tccccaaaga cggtcttaat cgcggcagtt tcactcttat cattcaattg
     1801 ggtgcttgtg ccgtgggcat tgatgtattg gatctcatcc aggccgagat tggcgtgctc
     1861 cagggcgttg cgcatacatc gcgcggctcc cgccccattt tctgcagggg cagaaatatg
     1921 gtaggcatca ttggttgcgc cataaccgct cacttctgcc aggatgcggg cgccgcgtgc
     1981 ccgggcatag tcttcagatt caaggacgag aatcgccgag ccttctccca tgacaaagcc
     2041 atcgcgatcc agatcgaagg gacgggaggc gcgctggggt tcatcattgc gggtggatag
     2101 agcagtcatc acgctcaaac cagccatggc gatggggaca attccggctt cgctcccgcc
     2161 tgccagcaca acatctgcct gcccgcggcg aataagctcc gcagcttcac ccactgcgtt
     2221 tgtgccagtg gcacaggctg tcac
//
LOCUS       DATMXK010000236         7531 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_45816, whole genome shotgun sequence.
ACCESSION   DATMXK010000236 DATMXK010000000
VERSION     DATMXK010000236.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7531)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7531)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7531
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45816"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1380)
                     /locus_tag="VN363_04390"
     CDS             complement(<1..1380)
                     /locus_tag="VN363_04390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83780.1"
                     /translation="MKPLHYFLLYLLAFLVLLGAASFQHSPGYMDAEYYLAGGMSITS
                     GSWQEPFIWNYLDDPVGLPHPAFAYWMPLASLLAAAGMKLGGSLSFTAGRLGFILVGA
                     GVAPLTAALAYRLDPRRTAALLAGLLAVCAGYYLPYLPAPDTFGLVMLLGGGFLFLCQ
                     LLGERSKSRANQPSLESPSGESPRWQDLAMVIGLGVIAGFMHLARADGLLWLAAGGLA
                     VLILPGSAWPRRLAHLGLLVLGYLVVMAPWMARNQAVFGSFFSAGGLRPLWITTYNEL
                     YTYPASLLTPQRWLASGLPALLAVRLKALGVNLQSLLAVQGQIFLLPLMLVGLWQLRR
                     AWAVRLGGLAWLAILGVMSLVFPHQGWRGGYFHSAAALQPLLWAVVPLGLSTFINWAG
                     AERRWNIPQAQRVFQVGLLVLAAGFSLVVSGGRIFGASPQNPGDSLLWDAPARRYERM
                     GAALEAQGLP"
     gene            complement(1421..2668)
                     /locus_tag="VN363_04395"
     CDS             complement(1421..2668)
                     /locus_tag="VN363_04395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 87 protein"
                     /protein_id="HWQ83781.1"
                     /translation="MEAQKRFLLNFFLLLLAAAAFGGLIWANIGYARQNPGGYDLIPR
                     WLGTRLYIMEGQNPYSEETSEAIQRQILGRLARPDEDQSLFVYPFYSFIVFLPFALFE
                     DFAVARGVLIAVQEVALLLIAGLGINLARWKLSPWMLGMVMLFSVGWYHSVRPVINTN
                     ISILVALFIMLALWAIREQHDVLAGFLLVLATIKPQMIVLIVPLILIWALTRRRFLIV
                     TSFSGILALIFLTSLLLLPGWPLDFLRQLMAYPDYTLPNTPGAIFADWLPGIGSQMGW
                     ALTGILAAVLVVEWIQAFNKDFRWLYWTACLTLVITNWIGIQTATENYIALLPAVILV
                     FAVWVERWRWLGRFMTGLSMLGLTGGLWWVFLATLTYRDGAPFQHSVMFFPVPLFLII
                     SLYWVRHWAITETRPLLDELRGS"
     gene            2854..4449
                     /locus_tag="VN363_04400"
     CDS             2854..4449
                     /locus_tag="VN363_04400"
                     /inference="COORDINATES: protein motif:HMM:NF021635.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-pyruvoyl-tetrahydropterin synthase-related
                     protein"
                     /protein_id="HWQ83782.1"
                     /translation="MKTHPSATTNPAYLFLALAILLGGYARLNQVLTSPFPLNDGGLF
                     YAMTQDLQANQYSLPEKTSYNQLELPFAYPPLPFYLAGGLNDLTGWELLEFFRLLPAV
                     FSILAIVAFYWLANDLAENRVHAGVATLIFALLPTTFDWLIMGGGLTRAPAFFFALLT
                     LHRIYLLYTRRRTGDVLWTAVLASLTILSHPETALHTVISAVVFFFFLERSKSGLIKS
                     LLVAGLVLGLTSPWWIPNLARHGLEPYLAAGSTGWHNFTALVQLFRFDLTNEYGLATI
                     GVLALIGLFWQLANSQVFLPVWLLVIFLSEPRSAPLYITPILALTASYSLLRILRFFK
                     PAKDATRVDSTSEIPLSSRAGRVLFALLFAQWTLSALVIPVIIAGTKTLSPADKTAFD
                     WVTANTLPQARFLILTGDDPFSDPTSEWFPALTGRVSVATVQGYEWEAAQDFEQIKQR
                     GRQVQECVQQTPACIKEWAANTGAEAGMEYDYILLRKLSGAGGWQLTPYDSGLEKLLV
                     LEEHALPVYQTPEVVILKVASQP"
     gene            4650..>7531
                     /locus_tag="VN363_04405"
     CDS             4650..>7531
                     /locus_tag="VN363_04405"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83783.1"
                     /translation="MSQSYSSTTIGRRQRLGRLLFLSIFLLGLFSPLNSSPAYAIDAP
                     NPLAPANGVTVTATGYAGSLAMPPNAFPEFSWTAVAGAGSYKLQISPDIAFTTKQEYT
                     TPLTHYTPTNLGPFPDGLYYWRVLVDWPEVSFPSVTRSFTRQWATPDNKPTLISPDEG
                     DVLEFFDAPAFSWLPVPGAAYYRLQISSNPDFIVLAVDNTTLSTTNQPVSKLSNGTYY
                     WRVLPFDTYNRQGTMSEARNFQMGYNQVPTLLEPQDHTYPTFTPTFRWTAVRGAQFYR
                     LQYSTDPAFAAAVTQIETRNTTFTPTTQLPNDVNYYWRVQTVSGSSLADWSAVWTFRK
                     QWYIKPVTLTPRNLYTSVRHPFFSWTPVPGASYYNIEIWSSNPSHSLSENVSNPWYTP
                     RQYWGDDSTYYWRVTPYERDNAKGVSSDTASYLSSYQMMAPMQVYPPYYYELDNYPAP
                     DLDVFTQPHEDRTVALPVFTWHRLTGPFPTGDTQAYVYRIEVNTDPTFIASPVWSVDT
                     ENTNATPTHTNYFYPVPRTDYYWRVCPLNALGGSCLEKAPGVPWWSQVWRARIDPGQM
                     LPPSDSGAPAVLLRPLHGAEIVETMPLFEWQALEGADRYEITISRDKAFTEIVESTLV
                     DYPAYAPTTSVAQRFLNRLNFGTFFWRVQAYDGATPVGEVSRTWRFQVASQSQWMPLR
                     TFGAEANRLVIGTDGNDTADDNYELRDLYASQDRDYWYFGFQATTAATNMTYALLLDT
                     DHVVESGGTFNPSPPGYDYNLTIIEDHQPEFVIFINQNDGAFSAAQTIIYPWEVGDFW
                     GTPDVLLNIDGAITFTDGYVNLKIPNTAIGYQEDSGSYAISLVSVNQSTLDGVDSVPS
                     DPNIPGGNYLSRFSSVSERMNLLRPANDIGGDPTTFASVPPFAWDYPVGGNGASPWAG
                     ARMKVYLDASYTNQIAEFTLVSDGAYYASSSHPWFNDFIGDNSYYWR"
BASE COUNT         1683 a         2298 c         2064 g         1486 t
ORIGIN      
        1 gggcagaccc tgggcttcca gtgcggcgcc catccgttca taacgccggg cgggtgcatc
       61 ccagagcaga gagtcccctg gattctgcgg gctcgcccca aagatccgac caccggacac
      121 caccaggctg aaacccgccg ccagcacgag caggccgacc tggaaaacac gctgtgcctg
      181 ggggatgttc cagcggcgtt ccgcaccggc ccaatttatg aaagtgctca agcccagcgg
      241 caccacagcc cagagcaggg gctggagggc tgccgccgaa tggaaatagc cgccgcgcca
      301 gccctgatga ggaaaaacca ggctcatcac acccaggatt gccagccagg ccagaccacc
      361 caggcggact gcccacgcgc gacgcagctg ccacaggcca accagcatca gcggcagcag
      421 gaaaatctgt ccctgaactg ccaggagact ctgtaaattg acccccagcg ctttgagacg
      481 tactgcgagc aaagccggca gcccggaagc cagccagcgc tgtggggtaa gcaggctggc
      541 agggtaggtg taaagctcat tataggtagt gatccacagt ggacgcagcc ccccggcgga
      601 gaaaaatgat ccaaagaccg cctggttacg cgccatccag ggcgccatga ccaccaggta
      661 gcccagaacc agcaggccga ggtgagccag gcgccgaggc caggcggagc cgggcaaaat
      721 caggaccgcc agccccccgg ctgccagcca gagcaggcca tcggctcgcg ccaggtgcat
      781 gaaacccgca atcactccga ggccaattac catcgccagg tcctgccagc ggggactctc
      841 acctgagggc gattccagag atggctggtt ggcgcgactt ttggaacgct cgcccagcag
      901 ctggcagaga aacaaaaacc cccctcccag cagcatcacc agcccgaaag tatccggcgc
      961 tggcagatag ggcaggtaat acccggcgca gactgccagc aggccggcca ggagagcagc
     1021 agtgcggcgg gggtctaagc gataggccag ggcggcagtc aggggagcga caccggctcc
     1081 aacgaggatg aaacccagcc gcccggccgt aaagctcagg ctgccgccca gcttcatccc
     1141 ggccgcggcg agtaaagacg ccagcggcat ccagtaggca aaggccggat ggggcaggcc
     1201 aacaggatca tcgaggtaat tccagatgaa gggctcctgc cagctgccgg aggtgatact
     1261 catcccgccc gccaggtaat attcggcgtc catgtaacca gggctgtgtt gaaaagatgc
     1321 cgcgcccagc agtaccagga aggcgagcag ataaagcagg aagtaatgca ggggtttcac
     1381 ggtgggggta gagtatgatc gaagaagaag ttgatcccga ttacgaaccg cgcagctcat
     1441 ccagcagggg gcgggtttca gtgatcgccc aatggcgcac ccagtacagg ctgatgatca
     1501 ggaataacgg cacgggaaag aacatcaccg aatgctggaa gggagcccca tcccgatagg
     1561 tcagggtggc caggaatacc caccacaacc cgccggtcag gccgagcatg ctgagcccgg
     1621 tcataaagcg ccctaaccag cgccagcgct cgacccacac cgcaaacacc aggatgactg
     1681 ccgggagtaa ggcaatataa ttttcagttg cggtctggat gccgatccag ttggtgatca
     1741 caagcgtaag gcaggctgtc caatacaacc agcgaaaatc cttgttgaaa gcctgaatcc
     1801 actcaaccac aaggaccgca gccaggatcc cggtcagcgc ccagcccatc tggctgccaa
     1861 tcccgggtaa ccagtccgca aatatggctc cgggggtgtt tggcaaggtg taatctggat
     1921 aggccatcag ctgacggaga aaatcgagcg gccacccggg gagcaaaagc aggctggtca
     1981 ggaaaatcag agccaggatt cccgaaaagg aggtcacgat caaaaaacgc cgccgggtca
     2041 gggcccagat caggatcaac gggacgatca ggacgatcat ctggggcttg atcgttgcca
     2101 gcaccagcaa gaaaccggcc aacacatcgt gctgttcgcg aatcgcccag agcgccagca
     2161 tgataaataa ggccaccaga attgagatat tggtattaat caccgggcgc acactgtgat
     2221 accacccaac cgaaaagagc atgaccatcc ccagcatcca gggagagagc ttccagcgcg
     2281 ccagattgat ccccaggccg gcaatcaaca gcaaggctac ttcctgcacc gctatcagca
     2341 ccccgcgggc gacggcaaaa tcttcaaata aggcaaaggg taggaacacg ataaacgaat
     2401 aaaacggata gacaaataaa gactggtctt cgtccgggcg ggcgaggcgg ccgaggatct
     2461 ggcgctggat cgcttcgctg gtctcttcgc tgtatgggtt ctggccttcc ataatataga
     2521 gccgcgttcc cagccagcgg gggattaaat catagccacc tggattctgg cgggcgtagc
     2581 cgatatttgc ccagatcaag cccccaaagg cggcggctgc cagcagtaac agaaaaaagt
     2641 tcagcagaaa gcgtttttgt gcttccattg cgctcagggg agctttccga ggccgggcag
     2701 ggatgcgttc ctggttaaca ttcagactta attgtacctt actgcccaaa cccggacctt
     2761 gtttttttgt aagctggcag ggcccaccct gagaatataa taagctacgg ctaagccacg
     2821 gctgtaacgg taaatatcgg aacatttttt acgatgaaga ctcacccatc cgcaaccacc
     2881 aatccagctt acctgttcct ggccctggct atcctcctgg gcgggtacgc ccgtctgaat
     2941 caggtgctca cttctccttt cccgctgaac gatggcgggc ttttttacgc catgacccag
     3001 gatctgcagg ccaaccagta cagcctcccg gagaagacca gttataacca gctcgagctg
     3061 ccgtttgcct atcccccact gcccttctac cttgccggag ggttgaatga cctgactggc
     3121 tgggagcttc tggagttctt tcgcctgctg ccagccgtgt tttcgatcct ggcgattgtg
     3181 gccttctact ggttagctaa cgatctggct gaaaaccggg tccacgccgg ggtcgcgacg
     3241 ctcatctttg ccctgctgcc aaccaccttc gactggttaa tcatgggtgg cgggctgact
     3301 cgcgccccgg catttttctt cgccctgctt accttgcacc gcatatatct gctctacacg
     3361 cgccggcgga ctggcgacgt actgtggaca gcagtcctgg catccctgac gatcctcagc
     3421 caccctgaga ccgccctgca tactgtgatc agtgcggtcg tctttttctt ctttctggag
     3481 cgcagtaaat ccggcctgat caaatctctg ctggttgccg ggctggtcct cgggctcacc
     3541 agcccctggt ggatcccgaa cctggcccgg cacggcctgg aaccctacct ggctgccggc
     3601 agcacaggct ggcacaattt caccgccctg gtgcagttat ttcgtttcga cctgaccaac
     3661 gagtacggcc tggcaacgat cggggttctg gcattgatcg gtcttttctg gcagctggcc
     3721 aacagccagg tctttctccc ggtgtggctg ctggtgatct ttctctcaga gccgcgcagc
     3781 gcacccctgt atatcacccc catcctcgcc cttactgcca gctacagcct gctgcgcatc
     3841 ctgcggtttt tcaagccggc aaaggacgct acccgggttg attccacatc tgaaattcca
     3901 ctatccagtc gggcagggag ggtccttttc gccctgttat tcgcccaatg gacgctgtcg
     3961 gccctggtca tcccggtcat catcgccggg acgaaaacgc tctccccggc ggataaaacc
     4021 gctttcgatt gggtcacggc caataccctc cctcaggcgc gatttctgat cctcaccgga
     4081 gacgatcctt tcagcgatcc cacctcagag tggtttcctg cattaaccgg cagggtcagc
     4141 gtggcgacag tccaggggta tgaatgggag gctgcccagg attttgagca gatcaagcag
     4201 cgcggccggc aggtccagga atgtgtccag cagaccccgg cctgtataaa ggaatgggcg
     4261 gccaatactg gcgccgaagc tggcatggaa tatgactata tcctcctgcg caagctgagt
     4321 ggcgcgggcg gctggcagct tacaccctat gacagcggtc ttgaaaaact gctcgtcctg
     4381 gaagaacatg ccctgcccgt ctaccaaaca cctgaagtgg tgatcctgaa agtcgcgtcc
     4441 cagccttagt tcggggtgcc tacctgattt atttgtaagt tgagtttagg ggggtcgtga
     4501 ctataatcat ggtcaaaggc gtgaatatga aaattagctc gggtttgggc ggctggcagg
     4561 aattgaaccc gccgccggtc gaacccggtt atagaagtca gaatttcgca gccctgcaaa
     4621 taaaataaaa ggtccaggtg ggaggtcaaa tgtcgcaatc gtattcgtct acaaccatcg
     4681 gtcgtcgcca gcgtctgggg aggctgctct ttctgagtat ctttctgctc ggcctgttca
     4741 gcccgctgaa cagcagcccg gcttacgcca tcgacgcccc caaccctctg gcgccagcca
     4801 acggggtgac cgtaaccgcc accggttatg ccgggtcatt ggccatgccg cccaatgcct
     4861 tccccgaatt ctcctggaca gcggtggcag gggccgggag ctacaagctg cagatcagcc
     4921 cggacatcgc tttcaccacc aagcaagaat acaccacgcc gctgactcac tacaccccca
     4981 ccaacctggg tccattcccg gatgggctgt attactggcg ggtgctggtt gattggcctg
     5041 aagtcagctt ccccagtgtt acccgcagct ttacccgcca gtgggcaacc cccgacaata
     5101 aaccaaccct gatcagccct gatgaagggg acgtcctgga atttttcgat gccccggcgt
     5161 tttcctggct gcctgtgccg ggtgcagctt attaccgcct gcaaatttcg tccaatcccg
     5221 atttcattgt tctggcggtg gataacacaa ccctgtccac cacgaaccag ccggtgagca
     5281 aactatcgaa tggcacctat tactggcggg tgctcccctt cgatacctac aatcggcagg
     5341 ggacgatgag cgaggcgcgc aatttccaga tgggctacaa tcaggtcccc acccttcttg
     5401 aaccacaaga tcatacttat cccaccttca cgcccacctt ccgctggacg gcggtgcgtg
     5461 gggcgcagtt ctatcgcctg cagtattcca ccgatccagc tttcgcggcc gcagtgaccc
     5521 agatcgagac ccgtaacacc acttttacgc ccacaaccca gcttccgaat gatgtcaact
     5581 attactggcg ggtgcaaacc gtctccggca gctcgctggc ggattggagc gcggtatgga
     5641 ctttccgcaa gcagtggtat atcaagccgg tgacactcac cccgcgcaat ttgtatacat
     5701 ccgtacgcca ccccttcttt agctggacac ctgtgcccgg ggctagttat tacaatattg
     5761 aaatctggtc atctaatcct tcgcatagcc taagtgaaaa cgtctccaac ccctggtata
     5821 cgccgaggca atattggggt gatgattcga cttactactg gagagttaca ccttacgaga
     5881 gggataacgc caaaggagtc tccagcgaca ctgcatccta tctcagcagt tatcaaatga
     5941 tggcgcccat gcaggtctac ccgccgtatt attatgagct ggataactat cccgcacctg
     6001 atctagatgt atttacccag ccacacgaag atcgcacggt agccctgccg gtcttcacct
     6061 ggcaccgcct gacgggtccc ttcccgaccg gagacaccca ggcctatgtc taccggatcg
     6121 aggttaacac tgatcccact tttatcgctt ccccggtatg gagcgtggat accgagaaca
     6181 ccaatgcaac acctacccac accaattatt tctacccggt tccccgaacg gattactact
     6241 ggcgggtctg cccgctgaat gccctgggtg ggagctgcct ggagaaagcg cctggtgtac
     6301 cctggtggag ccaggtgtgg cgggcgcgta tcgatcccgg gcagatgctt ccacccagcg
     6361 attccggggc gcctgccgtg ctcttgcgcc cgctacatgg cgccgagatc gtggagacga
     6421 tgcccctgtt cgagtggcag gcactggaag gcgctgaccg ctatgaaatt acaatcagcc
     6481 gagataaagc ctttacggag atcgtggaat ccaccctggt ggattatccg gcctatgcgc
     6541 ctaccaccag cgtcgcccaa aggttcttaa accgcctgaa cttcggcacc ttcttctggc
     6601 gcgtccaggc ttacgatgga gcaactcccg tgggtgaggt cagccgcacc tggcgcttcc
     6661 aggtggcatc gcagagccag tggatgccgt tacgcacctt tggagccgaa gccaaccgtc
     6721 tggtgatcgg caccgacgga aatgatacag cggacgacaa ctatgaactt cgcgacctgt
     6781 acgcaagcca ggacagagat tactggtatt ttgggttcca ggcgacaact gccgcaacga
     6841 atatgaccta tgcgctatta ctggataccg atcatgtggt tgagtcggga ggtactttta
     6901 atccatcccc tccaggctac gattacaacc tcaccataat agaggaccac caaccagaat
     6961 ttgtgatctt tatcaaccag aatgatggtg cattttctgc cgcccaaacg ataatttatc
     7021 cctgggaggt cggagacttc tggggcacac cggatgtttt gctcaacatc gatggcgcga
     7081 tcacctttac agatggctat gtcaatctga aaatccccaa taccgccatc ggttaccagg
     7141 aagattccgg tagctatgct atctcgctgg tgagtgtcaa tcagtcaacc ctcgacgggg
     7201 tggattctgt ccccagcgat ccgaatatcc cgggtggaaa ctatctcagc cgcttcagca
     7261 gcgtctccga gcggatgaac ctgctgcgcc cggcgaatga tatcggcgga gatccgacca
     7321 cctttgcctc cgtacccccc ttcgcctggg attatccggt gggcgggaac ggcgcctctc
     7381 cctgggccgg cgccaggatg aaggtttatc tggacgccag ctataccaac cagatcgctg
     7441 agttcactct ggtttctgac ggtgcgtact acgcgtcatc ttctcacccc tggttcaatg
     7501 atttcatcgg cgataacagc tactactggc g
//
LOCUS       DATMXK010000237         4149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_108109, whole genome shotgun sequence.
ACCESSION   DATMXK010000237 DATMXK010000000
VERSION     DATMXK010000237.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4149
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108109"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3424
                     /locus_tag="VN363_04410"
     CDS             <1..3424
                     /locus_tag="VN363_04410"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWQ83784.1"
                     /translation="ANTYRLDFLVIDAARQAIQQPAQAAGVQFSLPAVNKLINDLRLT
                     QAQQPDGSLDVVPGPYVEPVQLQVVCSNLWSHLPAGIEKITEEDIANIGNVDQSLAEY
                     YAERIAEVALQSGERERGIREWFEHQLITESGIRGQVLMGIGESKGISNDAIRLLEDS
                     HLIRAEKRLGAIWFELAHDRLIQPIRRNNAAWFQKNLSLLQRQASLWVQQNRFDTLFL
                     HDQALADAERWAAAHADEVSETDQEFLSNSLEQRARENAAREAAERERQLKLEAAQEL
                     ASQQAKAAARIRKVAYVLALVLVIAVVLGGLAFTAQQTATRNARIADQQRSTAQAAST
                     QAYNNALEAVANADAARAAASLARQNADIASNNAATAQAASTEAVAQQATAVYNAELA
                     REQEARAIIQANLARSRELASLALSFLKDDSILTLLLGKEAVDVSDSGQALDAMLRGL
                     QRNLTRQSERYDQFIPRQEIDIYALAASPDGQRLAWAGPDGLIRIWNLVAQEEEYTIY
                     MNQGFTITAITYSLDGKTLYTGDSGGNIALWNMETGRKLKDLLANINLVSAINSLALS
                     PDGTTLAYGGNTGGEPNVYTRNLETGVLRAFRVTRMGIEDALAVAWSPDGKILASAGR
                     DRAIHIWDPETRLEIETIRSVIQDNTLVDIYEGPIKSLVFSPNGKWLISGGEDNEGGV
                     KDKTLLMWDTAHWLDQPPIVFEGGPNSDVNLIKMSPDGQTLAAAYANGAIVTWNFSSQ
                     NLNEVITSHTQSVLALDFSQFEKSLLLVSSGFDRRIELNNLVAFDTLESPVSADKGNP
                     TRLALSPGDTLVLAGMADGPKLWEYSLTSAQEDQLEMDLPGDARNIFLSPDSSQIAWI
                     SVEDTIEIRSLDSGQAVATLPVPDVAITVTDGQGETSDQEETGQIDSLAFNWDGSLLA
                     GGYCSQRSRVNVPGTTDFIDTCLDREILVWNLPGGELIRRISTGQISAIGSLAFKPDD
                     PNILAAGYQDATIRIWDIEQGRASGLPLVGMGGPVTSLAFHLDGDVLASGSENNLIAL
                     WNLSPPQLIGDPFTGADGAVTGLAFSADNSTLFSGTDAGTISGWDIEAWKDLACQLTG
                     RNLTQAEWEQFFPNESYRATCEQYPLAALEVEATLSPATPTPGAPGTPTPTP"
     gene            3482..>4149
                     /locus_tag="VN363_04415"
     CDS             3482..>4149
                     /locus_tag="VN363_04415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83785.1"
                     /translation="MGSKEPDELLEGNELLYFNGIDGDTGQFALPPMTGNELSSFIRQ
                     ESQPDNLAELRYRYEESTKKSMGVREGIDPKQLDQTGWGVIFPAETDPAVKEALIDLL
                     NLRSQQAGGYFKIYAGGDGYRPGESKAQFLARHKAGPGAADPEKVPYYLLLVASPDEI
                     PYQFQSQLDVQYAVGRIHFATLQEYANYASSVVAAETGNLKLPRQASFFGVSNPGDKA
                     TSLSA"
BASE COUNT          923 a         1163 c         1232 g          831 t
ORIGIN      
        1 ggcaaacact taccggctgg attttctggt catcgatgct gcccggcagg cgatccagca
       61 gcctgcccag gcagccggcg tccagttcag cctgccggca gtcaacaagc tgatcaatga
      121 tctccgtctg acccaggccc agcagccgga tggcagtctg gacgtggtgc cgggtcccta
      181 tgtcgagcct gttcagctgc aggttgtgtg cagcaatctg tggagtcatc tgccagccgg
      241 gatagaaaaa atcaccgaag aggatatcgc caatattggc aatgtcgacc agtccctggc
      301 cgaatattac gccgagcgga tcgccgaggt tgccctccag agcggcgaac gtgagcgtgg
      361 aattcgagag tggttcgaac accagctcat caccgaatcc ggtatccgtg gacaggtgtt
      421 gatgggcatt ggtgagagta aagggatttc gaacgatgcc attcgcctgt tagaggattc
      481 acacttgatc cgggctgaga aacgcctggg ggcgatctgg tttgagttag cccacgaccg
      541 gttgatccag ccaattcgca ggaacaatgc cgcctggttt caaaaaaacc tgagcctgct
      601 ccagcgccag gcatccctgt gggtgcagca gaaccgcttc gacacattgt ttctgcacga
      661 ccaggcactg gcagatgccg agcggtgggc ggctgcgcat gccgatgagg tcagcgaaac
      721 cgaccaggag ttcctctcaa acagtctgga gcagcgcgct cgcgagaatg ctgcccgcga
      781 ggcagccgaa cgtgaacggc agctgaaact ggaagccgcc caggagctgg ccagccagca
      841 ggctaaagcc gcggccagaa ttcgcaaagt agcctacgtc ctggctcttg tgctggtgat
      901 cgcagtggtt ttgggaggcc tggcttttac agcgcagcaa actgcgacca gaaatgccag
      961 gattgctgac cagcagcggt ccacagccca ggctgccagc acccaggcgt ataacaatgc
     1021 cctggaagcc gtcgccaacg ccgatgctgc cagggccgca gcctcactgg cgaggcagaa
     1081 tgccgacatt gccagcaata atgccgctac cgcccaggct gccagcacag aagctgttgc
     1141 tcagcaggcg actgccgtat ataacgcaga attggccaga gagcaggaag ccagggcgat
     1201 cattcaggcc aatctggctc gctcgcgtga gctggcttca ctggctttgt ctttcctgaa
     1261 ggatgacagc atcctgactt tacttctggg aaaagaagcg gtcgatgtat ctgattcagg
     1321 gcaggcattg gacgccatgc tgcgcggctt gcagcgtaac ttaacccgcc agagtgagcg
     1381 ctacgaccag tttatccccc gccaggagat tgatatttac gcgctggcag ccagtcccga
     1441 cggtcagcgc ctcgcctggg ctggcccgga tggcttgatc cggatctgga atctggtggc
     1501 tcaggaagaa gaatatacca tctacatgaa ccagggtttc acgattactg cgatcaccta
     1561 cagcctggat ggtaaaacct tatacaccgg agattctggg ggaaatattg ctctctggaa
     1621 tatggaaacc gggcgtaagc tcaaagattt actggccaac atcaacctgg tctccgccat
     1681 caattcgttg gccctcagcc cggatgggac aaccctggca tacggcggca ataccggggg
     1741 agagccgaac gtatataccc gcaaccttga gactggcgtc ctgcgcgcat ttcgggttac
     1801 ccggatgggg atcgaagatg ctctggcggt ggcgtggtcg ccggacggga aaatcctggc
     1861 cagcgctggc cgggatcggg cgatccatat ctgggaccct gaaacccgcc tggagatcga
     1921 gacgatccgg tctgtcatcc aggacaatac tctggtcgat atctatgaag gtcctatcaa
     1981 gagcctggtt ttttcgccga acggcaaatg gttgatcagc ggcggggagg ataacgaggg
     2041 cggagtaaaa gataaaaccc tgttgatgtg ggatacagct cactggttgg accagccgcc
     2101 aatcgtattt gagggcggac cgaacagcga tgtcaacctg atcaaaatga gcccggacgg
     2161 gcaaaccctg gctgcggctt atgcgaatgg cgcgattgtc acctggaact ttagcagcca
     2221 gaatttgaat gaggtgatta ccagccatac acaatcggtg ctggccctcg atttcagcca
     2281 gttcgaaaaa tcgctcttgc tggtttcttc cggatttgac cggcggattg agctgaacaa
     2341 cctggtcgct ttcgatacgc tcgagtctcc tgtgagtgcg gataaaggta acccgaccag
     2401 gcttgccctc tcacccggcg atacgcttgt gttggccggg atggcagacg ggcccaaact
     2461 ctgggaatac agccttacca gtgctcaaga ggatcagctt gagatggatc taccaggaga
     2521 tgcccggaat atattcctca gcccggacag cagccagatc gcctggatat cggtagagga
     2581 tactatcgaa atccgatctc ttgattctgg acaggctgtc gccaccttgc ctgtaccgga
     2641 tgttgccatc accgtgaccg atggccaggg tgagacgagc gaccaggaag aaaccgggca
     2701 gatcgatagc ctggcgttca actgggatgg gagtttactc gctggtggtt attgcagcca
     2761 gcgcagccgg gtgaacgtgc ccggcaccac ggatttcatc gatacctgcc tggatcgtga
     2821 gatcctggtc tggaatttac ctggtggtga gcttatccgg cgcatctcaa ccggacagat
     2881 cagtgcgatc ggcagcctgg ccttcaaacc ggatgatccg aatatccttg cagccggcta
     2941 ccaggatgcc acaatcagaa tctgggatat tgagcagggg cgagccagcg gattacctct
     3001 ggttgggatg ggaggaccgg taaccagcct tgctttccac ctggatgggg atgtcctggc
     3061 ctcgggcagt gagaataacc tgatcgccct ctggaacctc agcccacctc agctgattgg
     3121 ggatcccttc accggagcgg atggagcggt taccgggctg gccttcagcg cagataacag
     3181 cacccttttc tcgggaacag atgccggcac gatctcaggc tgggatatcg aggcctggaa
     3241 agatcttgcc tgccagctca ccgggcgtaa tttgactcag gccgagtggg agcagttctt
     3301 cccgaatgaa agctaccggg ctacctgtga gcagtacccc ctcgctgccc tcgaagtaga
     3361 ggctacgctt tcgccggcaa cgccgacccc gggtgcaccc ggcacaccca cgcctacgcc
     3421 ctagccagtt tcttcgttag tggatttggt cacgggtttg gccagatgtt caggaggctt
     3481 tatgggatcc aaagaaccgg acgagctgct cgaaggaaat gagctgctgt atttcaacgg
     3541 catcgatggc gataccggcc agttcgcact gccaccgatg accgggaacg agctttccag
     3601 cttcatccgg caggagagcc agcccgataa cctggctgag ctgcgctacc gctacgagga
     3661 atcgacgaaa aaatcgatgg gggtgcgcga agggattgat cccaagcagc tcgatcaaac
     3721 tggctggggg gtgatttttc ccgccgaaac ggacccggcc gtgaaagagg ctttaatcga
     3781 tctgcttaac ctgcgcagcc agcaagccgg aggctacttc aaaatatatg ccggcgggga
     3841 tggttaccgg ccgggtgaat ccaaggctca gttcctggcc cgccacaagg ccgggccggg
     3901 agcagccgat cctgagaagg ttccttatta tttgctcctc gtagccagcc cggacgaaat
     3961 cccttaccag tttcaatccc agctggatgt gcagtatgcg gtggggcgga tccacttcgc
     4021 cacgctgcag gaatatgcga attacgccag cagtgtcgtt gcggctgaaa ccgggaatct
     4081 gaaactgccc cgccaggcat cctttttcgg tgtctccaac ccgggcgata aagccaccag
     4141 cctgagcgc
//
LOCUS       DATMXK010000238         2641 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_126424, whole genome shotgun sequence.
ACCESSION   DATMXK010000238 DATMXK010000000
VERSION     DATMXK010000238.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2641)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2641)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2641
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126424"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..298)
                     /locus_tag="VN363_04420"
     CDS             complement(<1..298)
                     /locus_tag="VN363_04420"
                     /EC_number="4.2.3.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004107 - chorismate synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009073 - aromatic amino acid family
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="chorismate synthase"
                     /protein_id="HWQ83786.1"
                     /translation="MNLRFLTAGESHGPGLLMILEGLPAGLPLAAEDIDQELFRRQTG
                     YGAGPRMKLERDRAEIISGLMDGVTIGSPVGIKINNLDHSRWANKPIPAFTTPRP"
     gene            complement(295..1461)
                     /locus_tag="VN363_04425"
     CDS             complement(295..1461)
                     /locus_tag="VN363_04425"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ83787.1"
                     /translation="MIQPAHRIASLSSHYFADLDQQIARLSAAGKDILRLDVGSPDLP
                     PPQAVIDCLHTSASRPDSHGYQAHTGTEALRQAWAGMYRRLHSVKLDPESEIVPLIGS
                     KEGIFHLHQALINPGDIVLVPDPGYITYIRGAQIAGGEIYTLPLLEENDFLPDLAGIP
                     AEIARRAKLLWLNYPNNPTSAVASLEFFHEAVEFAKQYDLLLAHDAAYTQVTYDGYQA
                     PSLLELPGASGVAIEFNTLSKSHNMAGWRVAAAVGRADVLRYLFQLKTNVDSSHFLPV
                     LEAAALAMDGDQTWIAGRNKIYQARRDCVAAGLDRLSFHYRLPQASLYVWFAVPDEMT
                     SAAFCQRALEYCGVSLTPGTIFGKGGEGFVRLSLTSPIEEIDLAMQRLEKEFAV"
     gene            complement(1458..2369)
                     /gene="aroE"
                     /locus_tag="VN363_04430"
     CDS             complement(1458..2369)
                     /gene="aroE"
                     /locus_tag="VN363_04430"
                     /EC_number="1.1.1.25"
                     /inference="COORDINATES: protein motif:HMM:TIGR00507.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004764 - shikimate 3-dehydrogenase
                     (NADP+) activity [Evidence IEA];
                     GO_process: GO:0009423 - chorismate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="shikimate dehydrogenase"
                     /protein_id="HWQ83788.1"
                     /translation="MNSSPANYYRLGLIGWPVGHSLSPPIHEAAFRATGTQGEYKLYP
                     VDPDTQNSSLSALLSQLRSGEIHGLNVTVPYKAVVAEMVDRLSDTARITGAVNTLSWQ
                     LGQIVGDNSDVPGFLQHLQRINVIPQEKSLRCVLLGAGGSARAVAFALASTGHQVILA
                     ARRIAQAEQLAAEISRSGQVSLPISVIDLDFRRINQVINPDLLINTTPVGMHPATAHS
                     PWPEEIPLPRACFVYDLIYNPAETRLLQQARQAGLPASNGLGMLVEQAAIAFQIWTGH
                     RPPTNVLYQAVGQNLPVSSTKTNPESA"
     gene            complement(2366..>2641)
                     /locus_tag="VN363_04435"
     CDS             complement(2366..>2641)
                     /locus_tag="VN363_04435"
                     /inference="COORDINATES: protein motif:HMM:NF012497.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83789.1"
                     /translation="ESDRIQALSELVRLGANITEQADGFVVRGKAKLEGGIGNPHSDH
                     RIAMALAISGLASRQQVTVQGAEIIAESFPGFTHALQSLGAQLEVTS"
BASE COUNT          541 a          715 c          841 g          544 t
ORIGIN      
        1 cggggcgggg agtggtgaag gcagggatgg gtttattcgc ccagcggctg tgatccaggt
       61 tgttgatctt gatccccacc ggcgagccga tggtgactcc atccataaga ccgctgatga
      121 tctccgctcg atcgcgttca agtttcatgc gcggtcccgc gccgtagccg gtctggcggc
      181 ggaataattc ctggtcaata tcttctgccg caagaggcag gccagccggg agtccttcca
      241 ggatcatcag taagcccggt ccgtgagatt caccggcggt aagaaagcga agattcatac
      301 tgcaaattcc ttctcgaggc gctgcatggc gaggtcaatt tcctcgattg gggaggtcag
      361 cgataggcgg acaaaaccct cacccccttt tccaaaaatg gtccctggcg tcaggctgac
      421 tccgcagtat tcaagggctc gctggcagaa agctgcagaa gtcatctcat ctgggactgc
      481 gaaccagaca tacagagaag cctgcggcag gcgatagtga aaactcaagc gatccaggcc
      541 ggcggcgacg cagtcacggc gagcctggta gattttgttt ctgccggcta tccaggtctg
      601 atccccgtcc atcgccaggg cagcggcttc cagaaccggc aggaaatgac tgctgtcgac
      661 attggttttg agctggaata aataacgcag tacgtcggcg cgtcccaccg ccgcggcgac
      721 tcgccagcca gccatattgt gggatttgga gagtgtattg aactcaatgg cgacccctga
      781 tgcacctggg agctccagaa ggctgggagc ctggtagcca tcgtacgtaa cctgcgtgta
      841 ggcagcatcg tgagcgagca gaagatcgta ttgcttggca aattcaaccg cttcgtgaaa
      901 gaactccaga ctggctacag ccgaggtagg attattggga tagttcagcc acaggagctt
      961 tgcccggcgg gcgatctccg ctggtattcc agccagatct ggcaggaagt cgttttcttc
     1021 cagaaggggc agggtgtaaa tctcgccccc ggcgatttga gccccgcgaa tgtaagtgat
     1081 atagcctggg tccgggacaa ggacgatatc tccgggattg atcagggcct ggtgaaggtg
     1141 gaaaatgcct tccttggagc cgatcagcgg cacaatctca ctctccgggt ccagcttgac
     1201 gctgtgcagg cgccggtaca tgcccgccca ggcctggcgt aaagcctcag tcccggtgtg
     1261 cgcctggtat ccatgagagt ctggacggct ggccgaagtg tgcaggcagt ctatcactgc
     1321 ttgcggcggg ggcaggtccg gtgagccgac atccagacga aggatatcct tcccggccgc
     1381 gctcagacgg gcgatttgtt ggtccagatc ggcgaaataa tggctgctca ggctggcgat
     1441 gcggtgggcg ggttggatca tgctgactcc gggtttgttt tcgttgaaga aactggcagg
     1501 ttttgaccga ctgcctggta aagaacattt gtgggtggtc gatggccggt ccagatctgg
     1561 aaggcgatgg cggcctgctc taccagcatt cccagaccgt tggaggctgg cagtccagcc
     1621 tggcgcgctt gctgcagcag ccgggtctca gctggattat atatcaggtc gtacacgaag
     1681 caggctctgg gaagaggaat ttcttccggc cagggagaat gagcagtggc cgggtgcatc
     1741 ccgaccgggg tggtattaat caacaggtcg ggattgatta cctggttgat ccggcgaaaa
     1801 tccagatcga tgacggatat cggcaggcta acctgcccgg accgcgaaat ctcggcagcc
     1861 agctgctcag cctgggcgat gcggcgggcg gcgagaataa cctgatggcc ggtgctggcc
     1921 agtgcgaagg caacggcgcg ggcagagccg ccggcgccca gcaggacaca gcgcaaggat
     1981 ttttcctggg ggataacgtt gatgcgctgt aggtgctgga ggaagcccgg cacatcactg
     2041 ttatcgccca cgatctgacc tagctgccag gacagcgtgt tgaccgctcc ggttatccgg
     2101 gctgtatcgg aaagccggtc gaccatttct gccacgactg ctttgtaagg gacagtcaca
     2161 ttcagtccgt ggatctcgcc agagcgcagc tggcttaaca gggcgctcag ggatgaattt
     2221 tgagtatctg gatcgaccgg atacagcttg tattcgcctt gagtaccggt ggcgcggaag
     2281 gcagcctcat gaattggcgg cgagaggctg tgcccgactg gccagccgat taatccaagg
     2341 cgataataat tcgcagggct gctgttcaag aggtgacttc cagctgtgcg ccgagcgatt
     2401 ggagggcatg cgtaaatccc gggaaggatt cggcgataat ctctgccccc tggacggtaa
     2461 cctgctggcg ggatgccagg ccggagatcg ccagcgccat tgcaatgcgg tgatcgctgt
     2521 ggggattgcc aatgccaccc tcgagtttag ccttcccgcg caccacaaaa ccatccgcct
     2581 gctcggtgat atttgctccc aggcggacca gctcggagag cgcctggatc cggtcacttt
     2641 c
//
LOCUS       DATMXK010000239         2096 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_68434, whole genome shotgun sequence.
ACCESSION   DATMXK010000239 DATMXK010000000
VERSION     DATMXK010000239.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2096)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2096)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2096
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68434"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..553)
                     /locus_tag="VN363_04440"
     CDS             complement(<1..553)
                     /locus_tag="VN363_04440"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83790.1"
                     /translation="MPTPSTPGISDIERITAIEDPVLRNLQITQCYYELGRALAERTG
                     GNANWCTFATWASKQAGQTIRKEDLGRTFEAALGSEENVREGLNKLKEAVSRLGKELN
                     IQELLRLIWKAYDPQAIFERSSAAVGRGNLKVFAEIGREFGRFIEECLPDESFTAANI
                     DRFVEQLRPGEPPDGQRYLRQAFQ"
     gene            complement(653..877)
                     /locus_tag="VN363_04445"
     CDS             complement(653..877)
                     /locus_tag="VN363_04445"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83791.1"
                     /translation="MAFSLDTTVGQLLDNPQAKAVLDQYLPGVSSNPMIAMVRGMSLN
                     MLLAMPQAAQLGMTKEKAEAILAEINKQIP"
     gene            complement(981..1853)
                     /locus_tag="VN363_04450"
     CDS             complement(981..1853)
                     /locus_tag="VN363_04450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83792.1"
                     /translation="MSFFRRLLGILVMIAGILGLVLSVAGLVGVWMVKPTVVSYADTT
                     ITTLNSSIGTSQEVMKITGEALGATIDSVDALSAMLDTTATSVEETQPVLDQVNIILG
                     ETLPSSLSSATDSLKTAQSAAGVLDSAIKSLDTFRTVLSAVPLVGSFVDTPSQPYNPE
                     VPLSESLGSLATELEDLPQQFTDMSANLRKADDSLASIQTNLVTMSVSVAQISTSLSD
                     YEAMIGQSQSSMDSLSVMLTNVQTNLPATMNGVGVVLSAFFVWLLIAQIVILTQGWEL
                     YQGTADRMESDQDD"
BASE COUNT          461 a          583 c          546 g          506 t
ORIGIN      
        1 gttggaaagc ctggcgcagg tagcgctgtc cgtccggtgg ctcgcccggg cgcagttgtt
       61 ccacaaaccg atcgatattc gccgcggtaa aggattcatc cggcaggcat tcctcgataa
      121 atcgcccaaa ctcgcgcccg atctctgcaa agaccttcaa attcccccgt cccactgccg
      181 cactggaacg ctcaaaaatt gcctgcgggt cataagcttt ccagatcagc ctcagcagtt
      241 cctggatgtt gagctccttg cccagacggc tgacagcctc cttcagctta tttagccctt
      301 cccggacgtt ttcttccgag cccagggcgg cttcgaaggt acgccccaga tcctctttgc
      361 ggatcgtttg cccggcctgt ttggacgccc atgtggcaaa cgtgcaccag ttcgcgtttc
      421 ctcctgtgcg ctcagccagg gctcgtccca gctcgtaata gcactgtgtg atttgcaggt
      481 tgcgcagcac cgggtcctcg atggctgtga tgcgctcgat gtcgctgatt cctggggtag
      541 aaggtgtagg catgctgcac tcgtagaaat taataaaaaa cccccgccag aagatctctc
      601 caggcagggg gttggtgatc aagggagagt taagcagttt cccgggacca tctcacggaa
      661 tctgcttgtt gatttctgcc aggatggcct cagctttttc cttggtcatg cctaattgtg
      721 ccgcttgtgg catcgccagc agcatgttca gtgacatccc acgcaccatg gcgatcatgg
      781 gattgctcga aacacccggc aggtactggt ccaacacagc cttagcctgg ggattgtcca
      841 acaattgtcc gaccgttgta tccagagaaa atgccattgc aggcctccat tcgttaggtt
      901 tggatatgaa ttaaatagat tgtatcatcg ccgctctgaa tcgcttgtta tgaacacatt
      961 atcaaaattc aactggttga ttaatcatcc tgatcacttt ccatccggtc ggctgttccc
     1021 tggtacagct cccacccctg ggtgaggatc acgatctggg cgatcagcag ccagacaaaa
     1081 aacgcgctca ggactacccc caccccattc atggtcgccg gtaaattggt ttggacgttg
     1141 gtcagcatta cggataggct gtccattgaa gactgagatt ggccgatcat cgcctcgtaa
     1201 tcgctcaggc ttgtggagat ttgagccaca ctgaccgaca tcgtaaccag attggtttgg
     1261 attgatgcca ggctatcgtc cgctttacgc aggttcgcgg acatgtccgt aaattgctgc
     1321 ggtagatctt ccagctcggt tgccagggac cccagcgatt cggacagtgg aacttccggg
     1381 ttgtatggct ggctgggtgt gtccacaaac gagcccacca gcggcaccgc gctcaaaacg
     1441 gtgcggaacg tatccaggga tttaatcgcg ctgtcgagga ctcctgcagc actctgagcg
     1501 gtttttaaag aatcggtagc cgacgacagg gatgaaggca gcgtctcgcc taaaatgata
     1561 ttgacctgat cgagtactgg ctgggtctcc tccaccgacg tagcggtagt gtccagcatt
     1621 gccgaaagtg catccacgct gtcaattgta gcccccaggg cttccccggt gatcttcatc
     1681 acttcctggc tggtgccaat gctggaattc agggttgtaa tcgttgtgtc agcataactg
     1741 accacagttg gcttcaccat ccacaccccc actaacccgg cgacactgag taccaatccc
     1801 aggatcccgg caatcatgac cagtattcca agtaaacggc gaaagaaact catggctcct
     1861 ccattgggtt ctcaggtttg gtctatttgc ccagcaccag gccggtcggg tcgatctggc
     1921 taagtatacg gcattcttcc tcagttgggg gtagggcgca ttgcctcaat aaaaatgtaa
     1981 ctttgagaaa atacgttgcc tcaaaaaaag tgttacccta ccgcagaacc ttaggtctgc
     2041 gagagggagc ttatgatgag tcagcggagc aagcgagaat tactggaaac cattcg
//
LOCUS       DATMXK010000240         3282 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_54520, whole genome shotgun sequence.
ACCESSION   DATMXK010000240 DATMXK010000000
VERSION     DATMXK010000240.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3282)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3282)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3282
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54520"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..967)
                     /locus_tag="VN363_04455"
     CDS             complement(185..967)
                     /locus_tag="VN363_04455"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83793.1"
                     /translation="MTVTALGAEFRRSLRLTRSYWLEYVADLILYILGFLLLSAVFQA
                     ASPTYGRAGMLSTLIGYTIWKICASVLEDIARVASDEARTGTLEQLFLAGWPPGLVFT
                     GRSLGFALSHTLRGLILAFILAGLLGLLQPVSPVAAVLFILTLLGACGLGFALAGLTL
                     IYKRIGGGLHLLWQMLVFFTGALAPITQPLLSGFAKLLPLTWGISALRASLLDGAGSA
                     ELWQRGLWQGLLINTLIYLILGVVLFSWGERRARQQGVLAHY"
     gene            complement(964..1944)
                     /locus_tag="VN363_04460"
     CDS             complement(964..1944)
                     /locus_tag="VN363_04460"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83794.1"
                     /translation="MSITDQPAIRVEHLIKSYAPERPNAVDGINFEIAKGTIFGLLGP
                     NGAGKTTTIKMILGLVLPSSGSVHLAGYDMSKERNRALRHAGAILEGSRNVYWRLSAR
                     ANLEYFGALRGLKGNSRAQRIQEVLELVELADRADEEVRYLSRGMQQKVALATAILHD
                     PDVLLLDEPTLGLDVQAARTIEKVIRQFVTQHGKTVVLTTHQMPLAERLSDSIFLIDK
                     GKRIAEGATREVIDRFGGQRETMEIELAGEMSPHILGRLQLDFTGLSATPHEENTILS
                     WIDSGSQKEFLRLQSELDEAGLTILQAGRRAATLEEVFVQLTSHPEKRTR"
     gene            complement(1941..>3282)
                     /locus_tag="VN363_04465"
     CDS             complement(1941..>3282)
                     /locus_tag="VN363_04465"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83795.1"
                     /translation="AYVIFQRKLLKVDFLINRLVVWFTLILLILIASILAFGVLVIAF
                     DLPAQLPIYGGVVAALIALPFSTLSKTVQDSVDRILYGSHYDYWTVTSSMSRQLAQTL
                     DRNQLISLLTEQLPQQMGIRQTELLLVDGGSLMSEQTTAKLPALSLDNNFCRELRRSH
                     VPMRASQAWNLISPDENKDWSHCAWAQIFAPLVFKDELQGILILGQRVTGDVYSDQDL
                     SIIATVAEQGALALVNVILFEKQRRLARQLVRSAEEERKRLASDLHDTVLQELFFIKQ
                     GLHQNPGNPALVDYLDESIQNLRQSIKTHRPPLLDKGLTLALQGLIEDVRKISGSKTA
                     IIWSTNIEGSPGLNDEQATVIFRIAQEALNNAVKHAKASLVEVSLEQCPEQITHLKVS
                     DNGSGLPWLQPDGNLDHAHFGLVLMQERAAMIDARLHINTLPGEGTTIELEVTA"
BASE COUNT          792 a          897 c          866 g          727 t
ORIGIN      
        1 caccagcacc agaagctgtt cttcctgccc ataatcccca ccgctgatcc cgttcagcag
       61 caccagggcg gcgatgaaca ccagaaaagt gtacaggaac caggacaggc tatctccggg
      121 gttggaccac tggatcagga agtaccgttt gatttcggcg cgcaggatcg tccacataag
      181 cagctcagta atgcgccagc acaccttgct ggcgggcgcg gcgctcaccc caggaaaaga
      241 gcaccacgcc caggatgaga taaatcaggg tgttgatcag caacccctgc cacaaacctc
      301 gctgccagag ctcggccgag ccagcaccgt cgagcaggct ggcgcgcagg gcgctgattc
      361 cccaggtcag cgggagtagt ttggcaaatc cggaaagcag aggttgggta atcggcgcca
      421 gggcgcctgt gaaaaagacc agcatctgcc agagcaggtg cagcccgccg cctatacgct
      481 tgtaaatcag ggtcaggccg gccagcgcaa atcccagacc acaagctcct agcaaggtga
      541 gaataaacag gactgcggct actggagaga cgggttggag caagcctaag agacctgcca
      601 ggatgaatgc caggattaat ccacgaaggg tatggctgag agcgaatccc aggctgcgtc
      661 cagtgaaaac caaaccaggt ggccaaccag ccaggaaaag ctgctccaga gtaccggtgc
      721 gggcttcatc tgatgcaacc cgggcaatgt cttccaacac gctggcgcaa attttccaga
      781 ttgtgtagcc aatcagtgta ctgagcattc ctgctcggcc ataggtgggt gaagcagctt
      841 gaaaaacggc gctgagcaac aagaaaccca ggatatagag gatcagatcg gcgacgtact
      901 ccagccagta ggaacgcgtc aggcgcaggc tgcgacggaa ctcagctcca agggcagtca
      961 cggtcatctg gtccgttttt caggatgaga agtaagctgg acaaagactt cctccagggt
     1021 ggcagcccgc cttccagcct gcaggatggt caggccagcc tcgtccagct cgctctgcag
     1081 gcgcaggaat tctttctgtg agccactgtc gatccacgat agaatcgtgt tttcttcatg
     1141 aggtgttgcg ctcaaacctg tgaaatcgag ttgcaaacgc cccaagatat gcggactcat
     1201 ctcacctgcc aattcgattt ccatggtctc tcgctgcccg ccaaaccgat caatgacttc
     1261 gcgagttgca ccttcggcga tgcgcttgcc tttatcaatt aagaaaatgc tgtcactcag
     1321 gcgctctgcc aggggcatct ggtgggtggt cagcacgaca gtcttgccgt gctgggtcac
     1381 aaactgtcgg atgacttttt cgattgtccg agctgcctgg acatccagtc ccagggtggg
     1441 ttcgtcgagc agcaatacat ccggatcgtg caagatggcc gttgccaggg caactttctg
     1501 ctgcatacct cgcgacagat agcgcacctc ttcatctgct cgatccgcca gctcgacgag
     1561 ttccagcact tcttggatcc gctgtgcgcg ggaatttcct ttgaggccgc gcaatgctcc
     1621 aaaatattcc aggttggcgc gtgctgaaag ccgccaatat acattgcgcg agccttcaag
     1681 gatcgccccg gcgtgtcgga gagcccgatt gcgttctttg gacatatcgt aaccagccag
     1741 atgcaccgaa ccactgctgg gcagtaccag ccccaggatc atcttgatcg tggttgtctt
     1801 gccggctccg tttggtccca gcaggccaaa gatggtccct ttggcaatct cgaagttgat
     1861 accatctacc gcatttggcc gctccggggc atacgatttg atcaggtgct caacacggat
     1921 agcaggctgg tctgtgatgc tcatgcagtc acctcaagct cgatggttgt gccttccccg
     1981 ggtaaagtat tgatatgcaa gcgcgcgtca atcatggcgg cgcgttcctg catcaacact
     2041 aaaccaaaat gggcatgatc caaatttcca tcgggttgta gccagggtaa acccgatccg
     2101 ttatcactca ctttcagatg ggtgatttgt tcaggacatt gctccaggct gacttcaacc
     2161 agactggctt tggcatgttt gacagcattg ttcaacgcct cctgggctat ccggaagatc
     2221 actgtggctt gttcgtcatt caatcctgga gatccttcaa tattcgtgga ccatatgatg
     2281 gcagtcttgg agcctgatat cttacgcaca tcttcaatca atccctgcaa tgcaagagta
     2341 agtcccttat caagtaatgg cggtcggtgg gttttgattg actggcgaag attttgaatc
     2401 gattcgtcca gataatccac cagggcaggg ttgcctggat tctgatgcaa accttgtttg
     2461 atgaagaaca attcctgaag cactgtgtca tggaggtcgc ttgccaggcg tttccgttct
     2521 tcctcagcgg agcgcaccaa ttgccgggct aaccggcgtt gtttttcaaa caagatgaca
     2581 ttgaccagcg ccagagctcc ttgctcagcc actgtggcaa tgatgctcaa atcctgatcg
     2641 ctgtacacat ctccagttac tcgctggccc aggatgagaa taccctgcaa ctcgtccttg
     2701 aaaaccaatg gggcgaaaat ctgcgcccaa gcacaatgtg accaatcttt attttcgtct
     2761 ggggagatca gattccatgc ctggctcgca cgcattggga cgtggctgcg cctcagctcc
     2821 cggcagaaat tattatctag tgagagggct ggtaatttgg ccgtcgtctg ttcggacata
     2881 agcgaaccac catctaccag tagtagttcg gtttggcgga tgcccatctg ctggggcaat
     2941 tgttcggtta acagactgat caactgattg cgatccaggg tttgcgccag ctgcctggac
     3001 atgctggaag tgactgtcca gtaatcgtag tgacttccgt ataagatacg atcaacacta
     3061 tcctgaaccg ttttcgacaa cgttgaaaat ggtaaagcaa tcaatgccgc taccacacca
     3121 ccataaattg gtagctgggc gggcaggtca aatgcaataa ccaacacgcc aaaagccaga
     3181 atagatgcga tcagaatcag cagaatcaga gtaaaccaca ctaccaaacg gttgatcaaa
     3241 aaatcaacct tcagcagctt gcgctggaag attacataag ca
//
LOCUS       DATMXK010000241         3496 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_128459, whole genome shotgun sequence.
ACCESSION   DATMXK010000241 DATMXK010000000
VERSION     DATMXK010000241.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3496)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3496)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3496
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128459"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..2406)
                     /locus_tag="VN363_04470"
     CDS             complement(205..2406)
                     /locus_tag="VN363_04470"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ83796.1"
                     /translation="MSRKRIALVTLGIMLSLFMASMESTVVATAMPTIVSQLGGLAIY
                     SWVFSAYMLASTTMMPIYGKLSDLYGRRLMYIIAMVLFLGGSMLSGQARSMEQLIFFR
                     ALQGLGAGGVQPLAFIIVGDLFNLEQRARVQGLFSGVWGVSSIVGPLIGGFLVDQVSW
                     RWVFYVNVLPGLLAAAIVWFAWKDRHLEGKGRVNVDYRGAALLTLGIVLLLLGLFELG
                     TSLGWALIATALALFGGLIWVERRAADPLLPLALFRERLFTVATLHGVLAGWAMFGSL
                     SFVPLFVQAVLGTSATSAGATLSPMLLGWVTASIIGSRLLLRISYRTLALLGMSLLTS
                     GALLMSQAGAGASQSSLMAYLAMMGVGMGLSVPPFLIAVQSTVRKNILGTATSTIQFS
                     RSMGGTLGVSVMGAFLSARLAAGLRAAGQDPAAVSLDSLLDPVASASLALEGSLRNIL
                     AGAISSVFVIAFIAAGCGLLVTLLAPRGRIAQLAAGRDIGEVGKEAAEPGAAKMGGLR
                     GPSRPAQANGKPFPVGAVRWQTGFSLEAYVSAMCTHQAEMRRRLGEVQLAEADLSDFA
                     QLRQPIHALVMSEDWCGDALMNLPILARLAQAAPQINVRVFVRSQEPDLDGYYRERGI
                     THIPVFTFLDAGFNEIGTWVERPKAARERLAEWLAAHPEIEAIRRDPGLEEEVRRDRL
                     REIGTRLVPEMEGWYAGGLQQATVDEVKEILQPVLILSELDHQEKTFTAVE"
     gene            complement(2574..2768)
                     /locus_tag="VN363_04475"
     CDS             complement(2574..2768)
                     /locus_tag="VN363_04475"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWQ83797.1"
                     /translation="MTAIDPVCKMEVEVETARYTSEHAGRTYYFCSADCQRSFEKDPH
                     RYLPEEHHGHGHDHESHSHP"
     gene            complement(2839..3309)
                     /locus_tag="VN363_04480"
     CDS             complement(2839..3309)
                     /locus_tag="VN363_04480"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83798.1"
                     /translation="MKPTLLAVFAHPDHIAVTRYTTQAFDLAGSSTDFPEAGLPWQPK
                     RLFYNAIPPSFFGEMRRKMQALGMDTSQFDGFDPRRHGLSDDQITTRIDVAQFADAKW
                     NALRCHGTQFGEDNLLRRLPEDELRQMMAREYFAQARPDATSGSQRSDELLLDL"
     gene            complement(3306..>3496)
                     /locus_tag="VN363_04485"
     CDS             complement(3306..>3496)
                     /locus_tag="VN363_04485"
                     /inference="COORDINATES: protein motif:HMM:NF013777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWQ83799.1"
                     /translation="VNPATLTQRLKTLERAGIINRYPISDGPRHVEYELTEKGKDLMG
                     VLSALTGWVHRWYPEEDR"
BASE COUNT          723 a         1065 c         1077 g          631 t
ORIGIN      
        1 cgttgggtca cgccggctgt tgccgccagc gtgatgagcg gctcgtaatt ggggtatacc
       61 agccggtcga tgatccccag gctggaaaac gggccggcgt cggcgcgccg ggcccactcc
      121 aggatctcgg ggcctgtgac gccgggaatt gtggctggca atccgatacc aatctgcacg
      181 tttacctcca ctcacgcttt tcagtcattc gactgctgtg aatgtttttt cttgatgatc
      241 caattcggaa aggattagaa caggctgcaa aatctctttt acctcgtcca ccgtcgcctg
      301 ctgcaaccct ccggcgtacc agccctccat ttcaggcacg agcctggttc cgatctcgcg
      361 cagccggtcg cgccgcacct cctcctccag ccccggatcg cgccggatcg cctcgatttc
      421 cggatgggcg gcgagccact ccgccaggcg ttcgcgcgcc gccttgggtc gttctaccca
      481 ggtgccgatc tcattaaaac cggcgtccag gaaggtgaag accgggatat gggtaatacc
      541 ccgctcccgg tagtagccat ccaggtcggg ctcctggctg cggacgaaga cccgcacatt
      601 tatctgcggc gcggcctgcg ccaggcgagc caggattggc agattcatca gcgcatcgcc
      661 gcaccagtct tcgctcatca ccagggcgtg gatgggctgc cgcaattgtg cgaagtcgga
      721 cagatcggct tcagccagct gcacttcgcc caggcggcgg cgcatttcag cctggtgggt
      781 gcacatggcg ctcacgtacg cctcgaggga aaaccccgtt tgccagcgga ccgcaccgac
      841 cgggaaaggc ttgccattcg cctgcgccgg gcgcgatgga cctctcaagc cgcccatttt
      901 tgccgcgcca ggctccgccg cttcctttcc aacctcgcct atatcccggc cggcggccag
      961 ctgggcaatc cgcccgcgtg gagccagcag ggtgaccagc agcccgcaac cggccgcaat
     1021 aaacgcaatc acgaatacgc tcgagatggc gccggccagg atgtttcgta gcgacccttc
     1081 cagggccagg gaggcgctgg cgaccggatc caggaggcta tccagagaaa ccgcggccgg
     1141 gtcctggccc gcagcccgca agccagccgc caggcgtgcg ctgagaaaag cccccatcac
     1201 gctcaccccg agtgtcccgc ccatgctgcg gctgaactgg atggtggaag tggcggtccc
     1261 caggatgttc ttccgcacgg tgctttgaac ggcaatcagg aatggcggca ccgacaggcc
     1321 catgcccacg cccatcatgg ccaggtaggc catcaggctg gactggctgg cgcctgcgcc
     1381 cgcctgcgac atgagcagcg cgccggaggt aagcaggctc atccccagga gcgccagggt
     1441 gcggtagctg atgcgtaaaa gcagccggct gccgatgatc gaggcggtca cccagcccaa
     1501 taacatgggc gatagcgtcg cccccgcgga cgtggcgctg gtccccaaga cggcctggac
     1561 gaacagcggc acgaaggaca ggctgccgaa catggcccag ccggccagga cgccgtgcag
     1621 ggtggctacg gtaaacaacc gctcgcggaa cagcgccagg ggtaagaggg gatcggccgc
     1681 ccggcgctcg acccagatca gtcccccgaa cagggccagc gccgtagcga tcagcgccca
     1741 gcccagagag gtgcccaact cgaacaggcc caggagcagc aagacaatcc cgagagtcag
     1801 cagagctgcg ccccggtaat ctacgttgac tcggcctttg ccttccagat ggcggtcttt
     1861 ccaggcgaac cagacgatgg cggcggcaag caggccgggg aggacgttaa cataaaacac
     1921 ccagcgccag gagacctggt cgaccaggaa gcctccgatc aagggaccga cgatggacga
     1981 gaccccccac acgcccgaga acaggccctg cactcgggcg cgctgttcta ggttgaacag
     2041 gtcgccgacg atgatgaagg ccaggggctg gaccccgccg gcgcccagac cctgcaaggc
     2101 gcggaagaag atcaactgct ccatgctgcg cgcctggccg ctcaacatcg agccgcccag
     2161 gaacaggacc atggcgatga tgtacatgag ccggcgcccg tacaggtcgg agagcttgcc
     2221 gtagatcggc atcatggtgg tcgaggccag catgtaagct gagaacaccc agctgtagat
     2281 cgccaatccc cctaactggg agacgatggt gggcatggcc gtggcgacta cggtcgattc
     2341 catcgaggcc atgaacaggc tgagcatgat gcccagcgtg acgagggcga tgcgtttgcg
     2401 tgacattagc agaggtgatc ccgaatcgtg accagttcca aatcgctttc atccgccagg
     2461 cgggcgcgct gtagggcggc ttctgacggt ggaagggtca atattgatgc caaagatagg
     2521 agtctggcta gcaggcataa gcataaggtc cgcaaagaag cgagagggat gatttaggga
     2581 tgtgaatggc tttcgtggtc gtgtccatgc ccgtgatgtt cttcgggcag atatcgatgc
     2641 gggtctttct cgaaggaacg ctggcagtct gcggagcaaa agtaataggt tctgcccgcg
     2701 tgctcgctcg tgtaccgcgc ggtttcaact tcgacttcca ttttgcaaac agggtcaatc
     2761 gcagtcatag atgtctccat ttcgaaaggt atgaattggt ggttggaatc taatgatgca
     2821 ggtgggtaac atttcgcctc acaaatcgag aagtaattca tctgatctct gagagccact
     2881 ggttgcatcg ggccgggcct gagcaaaata ctcacgggcc atcatctggc gcaactcatc
     2941 ttccggtaag cgacgtaaga ggttatcttc gccaaattgt gtcccgtgac atcgcagagc
     3001 gttccacttc gcatctgcaa attgagctac atcaatcctg gtagtgatct gatcatcgct
     3061 aaggccatgg cgtcgggggt cgaaaccatc gaactgggag gtatccatcc ccagcgcctg
     3121 catcttcctg cgcatctctc cgaaaaaaga tggcggaatg gcgttataga acagacgctt
     3181 cggctgccag ggtaggcctg cttctgggaa atctgtggag ctgccggcaa gatcaaaagc
     3241 ctgggtcgta taacgggtga cggcgatgtg atcgggatgg gcaaaaaccg caagtaaagt
     3301 cggtttcatc gatcctcctc cggataccag cgatgtaccc agcccgtcag ggcagacaac
     3361 acgcccatca ggtctttgcc cttttcagtc agttcgtatt ccacgtgacg cggaccgtcg
     3421 gagatgggat agcgattaat gatgccggcg cgctctaacg tcttgaggcg ctgggtgagc
     3481 gtggcagggt taacgc
//
LOCUS       DATMXK010000242         2357 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_130824, whole genome shotgun sequence.
ACCESSION   DATMXK010000242 DATMXK010000000
VERSION     DATMXK010000242.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2357)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2357)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2357
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130824"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..861
                     /locus_tag="VN363_04490"
     CDS             <1..861
                     /locus_tag="VN363_04490"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ83800.1"
                     /translation="RWDALYFPWNSAAIAYHALSELAIPVIVSCRGSQVNVRPHTPGN
                     EAFMHELRASLQQAAAVHCVSADILQEATKYGLDPARASVIRPAVDPGFFLPPTSPPA
                     SSRLVIITTGALTWLKGHEYDLLALRQLLDLGVEAEFHIIGAGPERQRVLYTIQDLAL
                     EGRVILHGKLPSAEVRDQLQAADVFLLASHSEGIANAALEAMSCGLPVVTTDCGGMRE
                     AVTDGVEGFVVPARDPAAMADALLRLAQDPALRLRMGQAARARVLRDFDLKDQIIAFT
                     ELMTAAQAGS"
     gene            861..2114
                     /locus_tag="VN363_04495"
     CDS             861..2114
                     /locus_tag="VN363_04495"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ83801.1"
                     /translation="MSIPATHRIVLVLAAFPVLSETFIVSKFLGLLQAGWDVHIVCTR
                     SDPAELAHFPQLKQQPDVKSRIHRTWPVHPRFIVFLLYPLALIRALLANPRGLLRYFR
                     RGAQAQGWRILPRFYLDFQLLALKPDLIHFEFGAMARGRADLNALLDCKEVVSFRGYD
                     LNFSGLDQPGYYDSVWQHADAFHFLGQDLRQRALRRGCPSQKPYALIPPAIDAVFFSP
                     GETGHPAQAVTPERPFRILSVGRLEWKKGYEFALQAVRELVERGIPCEYRILGGGPYL
                     PALAYARHQLGLEEVVEFLGPQPREQISTGLAWADAFLHAAVSEGFCNAVLEAQAMQV
                     PVVCSDADGLPENVLHGVTGFVVPRRDVRALADHLEKLAASPDLRAEMGREGRQRVIS
                     HFQLEDQIKRFASFYTQILESTNAN"
     gene            2104..>2357
                     /locus_tag="VN363_04500"
     CDS             2104..>2357
                     /locus_tag="VN363_04500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83802.1"
                     /translation="MQIDLIWLSRSGGPPAWPHGDMWSAAPTPAGVAGCVAQNLPACT
                     SDAWLFWDSELGAPDPEKIARALERPGDAWHAGLRLGLGG"
BASE COUNT          381 a          743 c          718 g          515 t
ORIGIN      
        1 cgttgggatg cgctctattt cccctggaat tccgccgcca tcgcctacca tgccttatct
       61 gaactcgcga ttccggtgat cgtcagctgc cgcggcagcc aggtcaatgt gcgcccgcat
      121 acccccggga acgaggcttt catgcacgag ctgcgggctt ccctccagca ggctgctgct
      181 gtacactgcg tctccgccga tatcctgcag gaagcgacta aatatgggct ggatccagca
      241 agggcttccg tcatccgccc ggctgtggat cccggttttt ttctccctcc cacttccccg
      301 ccggccagtt cgcgcctggt catcatcact acaggagcgc tgacctggct taaaggtcac
      361 gagtacgatc tgttggcgct gcgccagctg ctcgacctgg gcgtggaggc tgaatttcac
      421 atcatcggcg ctggccccga gcgccagcgg gttttataca ctatccagga cctggcgctg
      481 gaaggcaggg tcatcctgca cggtaagctg ccatccgcgg aagtgcgcga ccagctgcag
      541 gctgccgatg ttttcctgct cgccagccac agcgagggga ttgccaatgc cgccctggaa
      601 gcgatgagct gtgggctgcc cgtggtcacc acagattgcg gcgggatgcg cgaggctgtc
      661 acggatggcg tggagggctt cgtcgtcccg gcccgcgatc cggctgcgat ggcagatgcg
      721 ctgctcaggc tcgcccagga tcctgctctg cgcctgcgga tggggcaggc tgcccgggcg
      781 cgggtgctgc gagatttcga tctcaaggac cagattatcg ctttcacaga gttaatgacc
      841 gctgctcagg cgggcagctg atgtccattc cagccaccca tcgaattgtg ctggttctgg
      901 ccgcctttcc ggtcctttcg gaaaccttca ttgtcagcaa attcctcggc ctgctccagg
      961 ctggctggga tgtgcatatc gtctgcactc gcagcgatcc agccgaattg gcgcatttcc
     1021 cacagctcaa gcagcagccg gatgtgaaga gccggatcca ccgtacgtgg ccagtccacc
     1081 cgcgcttcat agttttcctg ctctaccctc tggcgctcat acgggctttg ttggcaaatc
     1141 cgcgtggctt gttgcgctat tttcgccgtg gcgcacaggc acaaggttgg cggatcctgc
     1201 cccgcttcta cctcgatttc cagctgctgg ccctgaagcc agatctgatc cattttgagt
     1261 ttggcgccat ggcacgcggc cgggcggatc tcaatgcttt gctcgactgc aaagaagtgg
     1321 tcagtttccg cggttacgat ctcaattttt ccggtctaga ccagcccggt tattatgatt
     1381 ctgtctggca gcacgccgac gccttccact tcctcggcca ggatttgcgc cagcgtgccc
     1441 tgcgccgcgg ctgcccgtcc caaaaaccct atgccttgat ccccccggca atcgacgctg
     1501 ttttcttctc gcctggtgag acagggcatc ccgcacaggc ggttactccc gaacgaccgt
     1561 tccgcatcct cagtgtgggc cgcctggaat ggaaaaaagg ttacgagttt gccctgcaag
     1621 cggtgcggga gctggtggag cgcggtatcc cctgcgaata ccgcatcctg ggtggtgggc
     1681 cttacttgcc ggccctggcc tatgcccgcc accagcttgg cctggaggag gtggtcgaat
     1741 tcctcggccc tcagcccagg gaacagataa gcaccgggct ggcgtgggcg gatgcattcc
     1801 tgcatgccgc ggtctcggag ggtttctgta atgctgtgct ggaggctcag gccatgcagg
     1861 ttccggtggt ctgctcggat gccgacggcc tgccggagaa tgtcctccac ggggtgaccg
     1921 gttttgtcgt tccccgccgg gatgtgcggg cgctggcaga ccaccttgaa aaactggctg
     1981 cctctcctga cctgcgggcc gagatgggca gagaaggccg ccagcgggtg attagccatt
     2041 tccagcttga agatcaaatc aagcgctttg ccagttttta cactcaaatc ctggaatcca
     2101 cgaatgcaaa ttgacctgat ctggctttcc cgatctggtg gtccccctgc ctggcctcac
     2161 ggggatatgt ggtctgccgc acccacgccg gcgggtgtgg ctggctgtgt ggcgcaaaac
     2221 ctgccagcct gcaccagtga cgcctggctc ttctgggaca gcgagctggg tgcgccagat
     2281 ccagagaaaa ttgcccgggc gctggagcgt cccggtgatg cctggcatgc cgggctccgg
     2341 ctgggcctgg gcggcag
//
LOCUS       DATMXK010000243         2099 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_64597, whole genome shotgun sequence.
ACCESSION   DATMXK010000243 DATMXK010000000
VERSION     DATMXK010000243.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2099)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2099)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2099
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64597"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..345)
                     /locus_tag="VN363_04505"
     CDS             complement(<1..345)
                     /locus_tag="VN363_04505"
                     /inference="COORDINATES: protein motif:HMM:NF012568.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl synthetase family protein"
                     /protein_id="HWQ83803.1"
                     /translation="MEDLTRTMLEAVEDELRQVVARANGSSLEEMHHMLAYHLGWEGE
                     GSGKQARGKRVRPLLLLLVCAAAGGKWRMALSGAAAVELIHNFSLIHDDIQDNSPLRR
                     GRPTVWTKWGIPQ"
     gene            complement(362..1393)
                     /gene="fni"
                     /locus_tag="VN363_04510"
     CDS             complement(362..1393)
                     /gene="fni"
                     /locus_tag="VN363_04510"
                     /EC_number="5.3.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560447.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004452 - isopentenyl-diphosphate
                     delta-isomerase activity [Evidence IEA];
                     GO_process: GO:0008299 - isoprenoid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type 2 isopentenyl-diphosphate Delta-isomerase"
                     /protein_id="HWQ83804.1"
                     /translation="MSKVTPIGARKTDHIRINLDEDVRSGLTTGLERFQFIHQALPEI
                     DLEGVDLGSEIFGRHLQAPLLISSMTGGTEQAGTINRTLAEAAQVCGIAMGVGSQRAA
                     LEQPDLAASFQVRSLAPDILLLANLGAIQLNYGYGIDHCRKAVEMIEADALILHFNAL
                     QEAVQPEGETRFAGLLDKIAQVCHVLPVPVIAKEVGWGFSKADACRLAEAGVAAIDVA
                     GAGGTSWSQVEMHRASDDSQRRLAAAFIDWGIPTSQAILNVRTAAPELLVFASGGLRN
                     GVEIAKCLALGASLGGMASPYLKAAVAGVDVTIQTIQEIRREIQVCMFGCGAGDLTAL
                     RSGKLEARD"
     gene            1667..1903
                     /locus_tag="VN363_04515"
     CDS             1667..1903
                     /locus_tag="VN363_04515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979028.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWQ83805.1"
                     /translation="MSDTYESVKAIIVDLLGVDETKVTMDARFREELEADSLDLVELI
                     MAFEDKFGGEISDEDAQTITTVGEAVKYLDSHAA"
BASE COUNT          451 a          636 c          568 g          444 t
ORIGIN      
        1 ctgggggatg ccccatttcg tccataccgt gggccgcccg cggcgcaggg ggctgttatc
       61 ctgaatatca tcgtggatca gagagaaatt gtggatcagc tccaccgcgg ctgccccgga
      121 taatgccatg cgccacttgc cgcctgccgc cgcacaaacc agtaacagca gcaaaggccg
      181 cactcgtttg ccgcgggcct gctttcccga gccttcgcct tcccagccca ggtggtaagc
      241 cagcatatgg tgcatctctt ccaggctgct gccattggcg cgcgccacaa cctggcgcag
      301 ctcatcttcc accgcttcga gcatggtgcg ggtgagatct tccatggtcc agcctcccgg
      361 gtcagtctct ggcttccagc ttgccggagc gtaatgcagt caaatccccc gccccgcagc
      421 cgaacataca tacctggatc tcgcggcgga tctcttgaat ggtctggatg gtcacatcca
      481 cgccggctac cgcggctttc aggtaggggc tggccattcc acccaggctg gcccctaacg
      541 ccagacattt ggcaatctct accccattcc gcagcccacc cgaggcgaac accagcagct
      601 ctggggcagc tgtgcgtaca ttcaggatcg cctggctggt ggggatcccc caatcgatga
      661 acgcggctgc cagcctgcgc tgggagtcat cgctggcgcg gtgcatttct acctgcgacc
      721 aggatgtacc gcccgccccg gcgacatcga ttgccgccac accggcctca gccaggcggc
      781 aggcatcggc ttttgaaaag ccccagccga cttccttggc gattaccgga actggcagca
      841 catggcagac ctgggcgatt ttatccagca gcccggcgaa gcgggtctcc ccttcgggct
      901 gcacagcttc ctgcagggcg ttgaagtgca ggatcagggc atccgcttcg atcatctcta
      961 cagctttccg gcagtggtcg atcccatatc cataattgag ctggatggct cccagattgg
     1021 ccagcaggag gatatccgga gccaggctgc gcacctggaa gctggctgcc agatcaggct
     1081 gctccagggc agcccgctgt gagccgaccc ccatggcgat cccgcaaacc tgggcggctt
     1141 ccgccagcgt gcggttgatc gtcccggcct gttcggtacc gccggtcatg gacgagataa
     1201 gcagtggcgc ctgtagatgc cgtccaaaga tctcgctgcc caggtccaca ccttccaggt
     1261 cgatctcagg cagcgcctgg tgaataaatt gaaagcgttc caggccggtg gtcagcccgg
     1321 aacgcacgtc ttcatccaga ttgatgcgaa tgtggtcggt tttgcgcgcc ccgatcggcg
     1381 taacttttga catgggtcgg tgttcctgtt ctgtggagtt atcttaccag tctggcacac
     1441 ccctgtcaat caatgacccg atgaactgcg ccacattgtg gcgctcttag ccttgacaga
     1501 acgcccggca agattacaat aagccacgaa atggcatatc gtaatggact cctaccgggt
     1561 cgctcgctgc ggacgcaaag ctataaaacc tgccggtaaa ggtacgctcg attgggcaag
     1621 ccagccacgg ctgatattct gcaagaacca ttgaggagac aatcgcatgt ctgatacata
     1681 tgaaagcgta aaagcaatta tcgtcgattt attgggagtt gatgaaacta aagtcaccat
     1741 ggatgctcgt tttcgcgaag agctggaagc ggattccctg gacctggtcg aattgatcat
     1801 ggcatttgag gataaatttg gcggggagat ctccgacgaa gatgctcaga cgatcaccac
     1861 cgtgggtgaa gctgtaaaat acctggatag ccacgccgcc tgaactcctg cccgaaccaa
     1921 attaaacagt accaacgcag ctcacctgag caaggtgggc tgtttgattc tttatcgctt
     1981 tgcacccaac ttcaccatca tatgcctcat aaaatgcaac ccaaattgct taaccaatat
     2041 ttaagaaaac tttcgtgtaa atacagtaac attctcaacg gccaaacgtt atataggtg
//
LOCUS       DATMXK010000244         4229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_57545, whole genome shotgun sequence.
ACCESSION   DATMXK010000244 DATMXK010000000
VERSION     DATMXK010000244.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4229
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57545"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..407)
                     /locus_tag="VN363_04520"
     CDS             complement(<1..407)
                     /locus_tag="VN363_04520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83806.1"
                     /translation="MADTQVTSLEFTLTPDQLLHDYRIAYQSRQVSLVGRREVMSGKA
                     KFGIFGDGKELPQMAMARAFRPGDFRSGYYRDQTFIFATGMGKVSEFFAQLYAHTDLK
                     ADPATGGRGMNAHFASRSLNPDGSWKDLTAQLLS"
     gene            complement(443..1576)
                     /locus_tag="VN363_04525"
     CDS             complement(443..1576)
                     /locus_tag="VN363_04525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006635 - fatty acid beta-oxidation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase family protein"
                     /protein_id="HWQ83807.1"
                     /translation="MSEVYILSAVRTPIGVGKPHGALQPLAPVDLGAAVMKAAVQQAG
                     VDPSRVQDIIWGCVTPTGDQGANLARLAALKAGFPVHVPGVTLNRMCGSSQQAVHFAA
                     QAILAGDMDLVIAGGTEMMSHQPLGADYPAVWPEDFPYELVHQGISAELMAQKWSHSR
                     DELDEFSYNSHLKAMQAIEQGRFESQILPIQRPDGKIICLDEGVRRPPDRQKMAELEP
                     VFKAGGIITAGNASQVSDGAAALLLASPEAVGRLNLIPRARILTRVVVGSDPVLMLDG
                     PIPATRLALKQAALDLEEIDVIEINEAFATVVLAWAAELRPDMQRVNPNGGAIALGHP
                     LGATGAILMTKLLYELERSGGQYGLQTMCIGHGMATATILERI"
     gene            complement(1678..2505)
                     /locus_tag="VN363_04530"
     CDS             complement(1678..2505)
                     /locus_tag="VN363_04530"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWQ83808.1"
                     /translation="MELITSKANPKIKQIRALRQHKARQAAGLLVVEGIRPVGEAIAA
                     HAQIESLVYAPDLLTSDYAQRLITEETARGVPAYPTTPDVFSSIAEKDNPQGLLAVVV
                     QPHTHLASLNPNNFSWGVAAISPQDPGNTGAILRTIDAAGASGLILIDNSVDPFHPTA
                     VRASMGALFWLPVAQTSFSEFSAWASTHAYQLYGTSAHASQDYREIPHYEHPCILLLG
                     SEREGLSLEQLNICQHRVRLPMAGRTTSLNLAVAAGILLYDMLEKQASQNLQNFTNH"
     gene            complement(2532..4205)
                     /locus_tag="VN363_04535"
     CDS             complement(2532..4205)
                     /locus_tag="VN363_04535"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing sensor histidine kinase"
                     /protein_id="HWQ83809.1"
                     /translation="MVVLTREQLEDRLAALHHASLQLVGDLSLETVLDRIVRMAREQA
                     GAEYAALGVVDEDGNLVKFLPVGIEQNQIDRMEHPPVGKGLLGMFYKERRTIRIPEIS
                     ADPRSAGFPPHHPEMHSFLGVPIILGDHLFGQIYLTNKQNYHEFTESDERVIETLATY
                     AAVAISNARLYNDLQIRDEALSQRNEDLALLNVTAAALTGSLDVDSILDQTLTRVMNY
                     LDVESGEIFLREENEKELRLVLFHGDNAEAFWTRDRFRVGEGIIGEVAETGKPLINFD
                     PRGNNLFLRKAVPEAGFKCMAVIPMLARGNVLGVISVATRQERCIGEREVNMLTAIAS
                     WAATTIENARLHRQGRRLAVLEERERIGMDLHDGIIQSIYGVGLALDYARMALDDNPQ
                     SARQKIDQSIEALNSTIHDIRAYILDLRPRLFHGQDLMEGLQRLVDEFKANSKIEVTL
                     AGPDAGLIDFPAENATILFHICQEALANVAKHSQARRVDVRLWTARDRVLLEVADNGG
                     GFDIRKMNFTLGHGLSNMHTRARKVGGDVEINSFPSEGTNVLAWVPRRA"
BASE COUNT          874 a         1194 c         1220 g          941 t
ORIGIN      
        1 gacaggagtt gagcagtcag gtctttccag ctgccatcgg gattcaggct gcggctggca
       61 aaatgagcgt tcataccccg cccgcccgtc gccggatcgg ctttcaggtc ggtatgggca
      121 tacaattgag cgaaaaactc gcttactttc cccatgccgg tggcaaaaat aaaagtctgg
      181 tcgcgataat agccggacct aaaatcgcca gggcggaaag cgcgcgccat cgccatctgg
      241 ggcagttctt tgccgtcccc gaagatgcca aatttcgctt tcccgctcat gacttcgcgc
      301 cgtcccacca ggctgacctg gcggctttga tatgcgatcc ggtagtcgtg caataactga
      361 tcgggggtca gggtgaattc aaggctcgtc acctgagtat cggccatgct tatccctcgc
      421 cttttcgttg gaataatatt tcttagatcc gttccaggat ggtcgcagtc gccatcccgt
      481 gaccaatgca catggtctgc agaccgtact gcccgccgct gcgttccagc tcatacagga
      541 gtttggtcat cagtatcgcc ccggtcgcgc ccaacgggtg tccgagggca atcgcgccgc
      601 cattggggtt gacacgctgc atgtctgggc gcaactcggc agcccacgcc agcacaactg
      661 tcgcgaaggc ttcattgatt tcgatcacgt cgatttcctc gagatcaagc gctgcctgct
      721 tgagcgccag ccgcgtggca gggattggtc cgtccagcat caataccggg tcagatccaa
      781 ccaccacgcg ggttaagatc cgggcgcggg gaatcagatt cagcctgccg actgcttcgg
      841 gagaagcaag cagcagagcg gctgcgccat cgctaacctg gctggcattt ccggcggtaa
      901 ttatcccacc ggctttgaag acgggttcga gctcggccat tttttggcgg tctggagggc
      961 ggcgcacacc ttcgtcgagg cagatgatct tgccatctgg gcgctgaatg ggcaggatct
     1021 ggctctcaaa ccggccttgc tcgattgcct gcatggcttt caggtggctg ttgtagctga
     1081 actcgtccag ctcgtcccgg ctgtgggacc atttttgcgc catgagctcg gcgcttattc
     1141 cttgatggac cagctcgtag ggaaaatctt caggccagac tgctggataa tccgcgccca
     1201 gcggctggtg agacatcatc tcggttcccc cggcgataac caggtccata tcgccggcga
     1261 gaatcgcctg ggcagcaaag tggacggctt gctggcttga gccacacatt cgattcagtg
     1321 tgactcccgg gacatgcacc gggaatccgg ctttcaaggc tgccaggcgg gcgagatttg
     1381 ctccctggtc gcctgttggg gtgacacagc cccagatgat atcctgtacg cgggagggat
     1441 ccacacctgc ttgctggaca gcagctttca ttactgccgc tcccaggtcc acaggggcga
     1501 ggggttggag agcgccatgg ggtttaccca ccccgatagg ggtgcggacc gctgaaagga
     1561 tataaacttc ggacataccg gtcgccctcc cttccgctat ccagcttgaa gtgaaccctt
     1621 gcggcacttc attacaaagc ataccacaaa agtccgatat agaactgggc ggtgcaatta
     1681 atgattggtg aaattctgga ggttctggct ggcttgtttt tccagcatgt cgtaaagcag
     1741 gatgcctgcc gcgacagcca ggttgagtga tgtggttctg ccagccatcg gcaggcggac
     1801 tcggtgctgg caaatgttga gctgctcaag gctcagaccc tcccgctcac tgccaagcag
     1861 caggatacac ggatgttcgt agtgagggat ttcccggtaa tcctggctgg catgggcgga
     1921 ggtgccgtag agttgatagg cgtgggtaga tgcccaggcg gaaaattcgc tgaaggatgt
     1981 ctgggcgact ggcagccaga ataacgctcc catgctggct cgcaccgcgg tagggtgaaa
     2041 aggatcgacg ctgttgtcga tcaggatcag cccgcttgcg ccggcggcat cgatcgtgcg
     2101 caggatcgca ccggtattgc cggggtcctg tggagaaatg gctgccacac cccaggagaa
     2161 attattaggg ttcaggctcg ccagatgggt gtgcggctgg actaccacag ccagcaggcc
     2221 ctgcgggttg tctttctctg cgatggagct gaaaacatcg ggtgttgtgg ggtatgccgg
     2281 gacgcctcga gcggtttctt cggtgatcag gcgctgcgca taatcactgg tcagcaagtc
     2341 tggagcgtaa accaggctct ctatctgggc gtgggcggcg atggcttcgc caaccggacg
     2401 gatgccttca accaccagca gtccggcagc ctggcgcgcc ttgtgctgcc gcagcgcccg
     2461 gatctgtttg atcttgggat tggctttgct ggtgatgagt tccatgctgc ctggaaaagg
     2521 atacctgcag gtcaggcgcg gcgcggcacc caggctaaaa cgttggtgcc ttcgctcgga
     2581 aaggagttga tctcaacgtc gccgcctact ttgcgcgccc gggtgtgcat attggagaga
     2641 ccatgcccta aagtgaaatt catcttccgg atatcaaagc ccccaccatt atcagcaact
     2701 tctaacaaga cccggtcacg ggccgtccac aaccggacat ctacccggcg agcctgagag
     2761 tgtttggcga cattcgccag ggcttcctgg cagatgtgaa ataatatcgt tgcgttctct
     2821 gccgggaagt cgatcaaacc ggcatccggt ccagccaggg ttacttcaat ctttgagttc
     2881 gccttgaatt catccacgag gcgttgtaaa ccttccatta aatcctgtcc atggaacaga
     2941 cgcgggcgta ggtccaaaat atatgcccgg atatcatgga tggtactgtt taaggcttcg
     3001 atcgattgat cgatcttctg gcgtgcagat tgggggttat cgtccagcgc catgcgggcg
     3061 taatcgagcg ccagaccgac cccataaatg gattgaatga tgccatcgtg caggtccata
     3121 ccaatccgct cacgttcttc caggactgcc agacggcgtc cctggcgatg gagtcgggcg
     3181 ttctcgatcg tggtggctgc ccacgaagcg atggcggtga gcatgttcac ctcgcgctcg
     3241 ccgatacagc gctcctggcg ggtggcaacg ctgatcacac cgaggacatt gccccgggcg
     3301 agcatcggga tgaccgccat acacttgaag ccagcttcag gcacagcctt gcgcaggaag
     3361 agattatttc cccgtgggtc aaaattaatc agcggcttac cggtctctgc aacctcccca
     3421 ataatgcctt cgccgacccg aaaccggtca cgcgtccaga aagcctcggc attatcgcca
     3481 tggaataaaa ccaagcgcag ctctttctcg ttctcttcgc gcagaaatat ctcgccggat
     3541 tcaacatcca gataattcat cacccgggtg agggtctggt ccaggatcga atcaacatcc
     3601 agtgagccag tcagcgcggc agcagtgaca tttaataagg ccagatcttc attgcgctga
     3661 ctcaacgctt cgtcgcggat ttgcagatcg ttgtacaggc gggcattcga gatcgcgacc
     3721 gcagcatacg ttgccagggt ttcaatgacc cgctcatcgg attcagtgaa ctcgtggtaa
     3781 ttttgtttgt tggtcaggta gatctggccg aagaggtgat ctcccaggat gatgggcaca
     3841 cccaggaatg aatgcatttc gggatggtgg ggaggaaagc cagcgctgcg tgggtcggcg
     3901 ctgatctcgg gtatccgaat cgtgcgccgc tccttataga acatccccag caatcctttg
     3961 cccacaggcg ggtgttccat ccggtcgatc tggttttgtt ctatccctac cgggagaaac
     4021 ttaaccagat taccgtcttc atccactacc cctaatgcgg catactcagc tccggcttgt
     4081 tcgcgagcca tccggacaat gcgatctaat acagtttcaa gggatagatc ccccaccaac
     4141 tgaagactgg catgatgtaa tgcagcaaga cgatcttcaa gctgttcacg ggtcaggaca
     4201 accatttaag acacctcata gctctgtct
//
LOCUS       DATMXK010000245         2421 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_126062, whole genome shotgun sequence.
ACCESSION   DATMXK010000245 DATMXK010000000
VERSION     DATMXK010000245.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2421)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2421)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2421
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126062"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..346)
                     /locus_tag="VN363_04540"
     CDS             complement(<1..346)
                     /locus_tag="VN363_04540"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWQ83810.1"
                     /translation="MTLEFVLFIILALVAVAAALGMILSRNTVYSALFLILNFGTVAV
                     FYLMLNAAFLAMAQITVYAGAIMVLFLFVIMLLGAEMIPGEDKLRWQKPLAIVLGVVL
                     LAEAGYILINRSG"
     gene            complement(426..929)
                     /gene="nuoI"
                     /locus_tag="VN363_04545"
     CDS             complement(426..929)
                     /gene="nuoI"
                     /locus_tag="VN363_04545"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559263.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016651 - oxidoreductase activity, acting
                     on NAD(P)H [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoI"
                     /protein_id="HWQ83811.1"
                     /translation="MVREILRGLGTTFREMLTKPVTVQYPEERRPVATRFKGRHFLER
                     YENGLEKCIGCSLCAAACPADAIFVEADLNTDAERYSPGERYARVYEINMLRCIFCGY
                     CEDACPTEAIILGDNYELSFTDRRQAIYAKEMLLVPVPPGGEPTPQVTEPGKFTRSIP
                     VMLDPTD"
     gene            complement(933..1943)
                     /gene="nuoH"
                     /locus_tag="VN363_04550"
     CDS             complement(933..1943)
                     /gene="nuoH"
                     /locus_tag="VN363_04550"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559262.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWQ83812.1"
                     /translation="MEAALIWEWIIKSLLLIIIMLVGFAYVTFYERRVLARIQVRVGP
                     NRAGPDGWLQPVADGIKLIFKEELIPAMADKKIFILAPILTVVPALILLGVVPWGRTV
                     QLFGRSIDLYLVDVNVAVLYITAVASISVYGIVLAGWSSSNKYATMGGIRSTAQMISY
                     ELALGLSFVGPILLSGTMSMLGIVDKQQEIGWFVLYQPVAFIIFFIATLAETNRAPFD
                     MPEAEQELTAGYHTEYSGMKFALFYMAEYDKMIGVSAIAATLFLGGFDGPFVNQVPWL
                     GPLYLFIKIAAMLFLMIWVRATLPRIRYDHLMAFGWKVLLPLSLLNIMVTAVVIVLVG
                     GG"
     gene            complement(1962..>2421)
                     /locus_tag="VN363_04555"
     CDS             complement(1962..>2421)
                     /locus_tag="VN363_04555"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWQ83813.1"
                     /translation="IVGRGDLYYGGTTYENKQGLGVQLAPLAQTGQPISLGWVKPKEL
                     DLGVGSSGGLVGVPVTLLYDRGTTLLPSDLLHQRMPAPYVCLHPEDARNLRLKQGDAV
                     EVSTGGAHVLVTIKVDETIPAGIVLVPRSMGIPLNSPANLAIQPAEKITA"
BASE COUNT          645 a          683 c          588 g          505 t
ORIGIN      
        1 tcccagatcg attgatcaga atatagccag cttcggctaa caaaacaacc cccaggacga
       61 tggccagagg tttttgccag cggagtttat cctcgcccgg gatcatctcg gcgcctaaga
      121 gcatgatcac aaacaaaaac agaaccatga ttgcgccggc ataaaccgtg atctgggcca
      181 tagccagaaa agccgcattt aacatcaaat aaaacacagc cacggtgccg aagtttagga
      241 ttaagaacaa ggcggaatag accgtattcc ggctgaggat catgcccaga gcggcagcga
      301 cagctaccag ggctaaaata atgaacaaga cgaattcaag agtcatgcac gctcactcac
      361 tcaattagat tttacctgcc ggagggtcca ggttggcgct gagctgctcc agcaggcggg
      421 ctcaatcaat ccgttggatc taacatcacc gggatcgagc gggtaaactt cccgggctcg
      481 gtgacctgcg gcgtcggctc gcctccgggc ggcactggaa ccaacagcat ctcttttgca
      541 taaatcgcct ggcgccggtc cgtaaaagaa agctcgtaat tatcgcctaa aatgatcgcc
      601 tctgttgggc aggcatcctc gcagtaacca caaaagatgc agcgcagcat attgatctca
      661 tataccctgg cgtagcgttc gccgggcgag tagcgctcag catcggtatt aaggtcagct
      721 tcgacaaaaa ttgcatccgc cgggcaagcc gcggcgcaga gtgagcaacc gatacatttt
      781 tccaacccat tttcatagcg ctctagaaaa tgacgccctt tgaaccgggt cgcaaccggg
      841 cgcctttcct ccgggtattg aactgtaacc ggtttggtca acatctctcg gaacgtggtc
      901 cccaggccac gtaaaatctc tctcaccatg gtttaacctc ctccaaccaa tacgattact
      961 acggcggtaa ccataatatt taataacgat aaaggtagaa gtactttcca gccaaatgcc
     1021 atcagatggt cataacgaat ccggggtaga gtcgcccgca cccagatcat caagaacagc
     1081 attgcggcga tttttatgaa taaatagagt ggtcccagcc agggcacttg attgacaaac
     1141 ggaccatcaa agcctcccag gaacagggtc gcggcgatgg cgctgacccc gatcattttg
     1201 tcgtactccg ccatatagaa taaggcgaat ttcatcccgg aatattccgt atggtagccg
     1261 gctgtcagct cttgctcggc ttcaggcata tcgaaaggcg cccggttcgt ctctgccagc
     1321 gtggcgataa agaatatgat gaatgctacc ggttggtaga gaacgaacca accgatctct
     1381 tgctgcttat cgacgatccc cagcatgctc atcgtaccag acagcaggat aggaccaaca
     1441 aacgacaggc ctaacgccag ctcgtagctg atcatctggg ctgtcgagcg gatcccgccc
     1501 atcgtggcat atttgttgct ggatgaccag cccgccagaa cgatgccata cactgagata
     1561 gaagcgactg cggtaatata taagaccgca acatttacat caaccaggta aaggtcaatc
     1621 gacctgccaa acaactggac cgttcgaccc caaggaacaa cccctaatag aatcagagcc
     1681 ggcacaaccg tcaggattgg ggccaggata aaaatcttct tatctgccat ggctggaatc
     1741 agctcttcct tgaaaatcag cttgatccca tccgcgacag gctgaagcca gccatctggt
     1801 cccgcccggt taggtcccac ccgcacttga atccgtgcca gcacccgccg ttcgtagaat
     1861 gttacgtagg caaagccaac cagcatgatt atgatcaaca acaatgattt aatgatccat
     1921 tcccagatca aagcagcctc catggttgat tcattgccgc cttaagcagt aattttttct
     1981 gctggctgga tagccaggtt cgcggggcta ttgagcggaa tacccatgct ccgcggcacc
     2041 agaactatcc ctgcagggat agtctcatcg accttgatgg taaccagcac atgggcacca
     2101 ccggtagaaa cctccaccgc gtcgccttgc ttcaagcgta aattacgcgc atcttctggg
     2161 tgcaaacaca catatggggc aggcatacgc tggtgcagca aatcagacgg taaaagcgtg
     2221 gtgccccgat catagagcag ggtaactgga acacccacca gtccaccgga cgagccaaca
     2281 ccgagatcaa gctctttcgg tttcacccag cccagggaaa tcggctgacc tgtttgagcc
     2341 aggggagcta attgtacgcc cagaccctgt ttgttttcgt aggtcgtccc gccataatag
     2401 agatcgccgc ggcctacaat c
//
LOCUS       DATMXK010000246         4214 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_48801, whole genome shotgun sequence.
ACCESSION   DATMXK010000246 DATMXK010000000
VERSION     DATMXK010000246.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4214)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4214)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4214
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48801"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..499
                     /locus_tag="VN363_04560"
     CDS             <1..499
                     /locus_tag="VN363_04560"
                     /inference="COORDINATES: protein motif:HMM:NF015085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cytochrome c maturation protein CcmE"
                     /protein_id="HWQ83814.1"
                     /translation="ASSASRVKFIIGGLLIVAAIVYLIVSSTQASAQYFLTINELNSK
                     GSEVAGRELRISGAVIGDTIQYDAQKLALRFQVADIPGDNSEIEAQGGLAKVLHDAVV
                     DPDRARINVVYSGVKPDLLRDEAQAIMTGHMGEDGIFQANELLLKCPTKYEDAVPDQV
                     EQAGS"
     gene            623..2677
                     /locus_tag="VN363_04565"
     CDS             623..2677
                     /locus_tag="VN363_04565"
                     /inference="COORDINATES: protein motif:HMM:NF027651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme lyase CcmF/NrfE family subunit"
                     /protein_id="HWQ83815.1"
                     /translation="MVANFGFGALVITFLLTIYGSFAAIYGARKNNPRWVGSARLAML
                     LTFPLLSLSALALIYLLINEHYEVEFVASVTSSSMPLYLKITAWWGGQSGSLLFWAWL
                     MSAFASAVTLRKWHRDGEFLPWVILVAMVTLAFFLILIIFFENPFQRIWQLPNGAIEL
                     GMFQPANATLVYQADGRGLNPLLRHPGMVIHPPMLYLGFVSFVIPFAFAIAALVTGRT
                     DDRWIRVTRRWTLVAWLFLSLGLILGSRWAYDVLGWGGYWAWDPVEIAAFMPWLTGTA
                     FLHSVMIQEKRGMLKHWNMILIILTYSLVIFGTFLTRSGVLSSVHAFAQSAIGPLFFA
                     FIGITFIISLALLLHRWESLKSESHMTSLLSREALFLLNNLLFLGILVICFWGVIFPL
                     ISELVTGQKVTVGPPFYERATGPLFAGLLLLMGVAPLAAWRHSTGKTLGKAIWKPTIF
                     AVLVTAVLVALGVRSLPALLTFLLCALVLSVTLYEFWRGAWARHRTSGESLPVALWNL
                     AGRNRRRYGGYIIHIGIVLMAVGIIGIEVFQTETQGTVPEGGELQLGEYTMRFDSLAV
                     FDTDDRNVARAVVSVFKDGDYVGEYYPRRDYYYEAQQSMTIPGVRSTLEDDFYVLLVD
                     WEAISSNGVTFKVFHNPLINWLWLGGLVFILGTMVAAWPDKDPEPAAVRTRRRSYAPI
                     EA"
     gene            2727..3275
                     /locus_tag="VN363_04570"
     CDS             2727..3275
                     /locus_tag="VN363_04570"
                     /inference="COORDINATES: protein motif:HMM:NF015853.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c-type biogenesis protein"
                     /protein_id="HWQ83816.1"
                     /translation="MKRTALVVFQVIILLAFGSSLALAAEPAPWLQDGVPRQPTDDEV
                     NAVAKQLYCPVCENIPLDVCPTLACEQWRSLIREKLAAGWSEEEIKTYFVNQYGDRVL
                     ATPPTRGLNWLVYIIPPAAILAGAFILFRALRAWRKPVEGVESETPALAEAGIPDEYT
                     ARLEDELRKHQAGSSTVKRDEL"
     gene            3272..3844
                     /locus_tag="VN363_04575"
     CDS             3272..3844
                     /locus_tag="VN363_04575"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWQ83817.1"
                     /translation="MMNEQVESTPEVGSARPRLRWGRILVWTGLLGLLALMAMGLLNT
                     QRGPVLIGQNAPQFVLSTFDGREINTQDLAGKVVVVNFWASWCKPCEQEAADLEAAWQ
                     IYAPTEQVVFLGVDYVDTEPEALGYLQKFNITYANGPDLRTRISQAYRIRGVPETYFI
                     DRQGKLADVQIGPFSNLAQITGKIEPLLAP"
     gene            3892..>4214
                     /locus_tag="VN363_04580"
     CDS             3892..>4214
                     /locus_tag="VN363_04580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83818.1"
                     /translation="MDIGSILIILALLIPVVLFIARPFYEPRLIEQEGLNQHEDHDLS
                     GLLAERDRILNALQELEFDHVLGKIPDEDYPAQRAWLVQQGADVLRRLDQIQASDSLL
                     AGKPEG"
BASE COUNT          843 a         1109 c         1227 g         1035 t
ORIGIN      
        1 agccagctct gccagccggg taaaatttat catcggtggt ctgttgattg tcgctgcaat
       61 cgtttatttg attgtttcat ccactcaagc cagcgcccaa tattttctaa ccatcaatga
      121 acttaattcg aaaggcagtg aagtagcagg acgagaactg cggatttccg gcgcagtgat
      181 cggggacacc atccagtatg acgctcaaaa gttggcatta cgctttcaag tagcggatat
      241 ccccggcgat aacagcgaga tcgaggcgca aggcggtctt gcaaaagtac tccatgatgc
      301 agtggtcgat cctgaccggg cgcggatcaa cgtggtctac tcgggtgtca agccagattt
      361 actgcgggat gaagcgcaag ccataatgac tggccatatg ggcgaagatg gcatcttcca
      421 ggccaatgag ctgcttctga agtgtcctac caagtacgaa gatgcagtgc ccgaccaggt
      481 ggagcaggct ggctcgtaaa ccgcgagcgt tcagcctgga taaatttttg gatatcccct
      541 tttagagcgt ctggtgctcg caccctgagc agcggacgtt tcctatctaa aaagctgatt
      601 tactaaatta ctgggagtcc ttatggttgc taattttgga tttggcgctc tggtaatcac
      661 ctttttattg acgatttacg ggtctttcgc agccatttac ggcgcccgga agaataaccc
      721 gcgctgggtc ggcagcgccc gcctggcaat gctcttaact tttccactgc tgtctctttc
      781 tgcattggct ttgatctatt tattgatcaa cgaacattat gaagtcgaat ttgttgcttc
      841 ggtgaccagc agcagcatgc ccctgtattt aaagatcacc gcctggtggg gaggtcaatc
      901 gggatcgctg ctcttctggg catggctgat gtcagccttc gcctcagcgg taactttgcg
      961 gaagtggcat cgcgatggcg aattcttgcc ctgggtgatc ctggtggcaa tggtcacgct
     1021 ggcgtttttc ttgatcctga tcattttctt cgagaatcca ttccagcgca tatggcagtt
     1081 acccaatgga gcgatcgagc tggggatgtt ccagccggcg aatgcaaccc tggtctacca
     1141 ggcggatggg cgtggattaa acccgctcct gcgccacccc ggaatggtca tccatccccc
     1201 catgctctat ctggggtttg tgtctttcgt gatccccttc gccttcgcca ttgcggcgct
     1261 ggtcaccggg cgcaccgatg accgttggat ccgcgtcacc cggcgctgga cgctggttgc
     1321 ctggttgttt ttatccctgg gattgatcct gggcagccgc tgggcttacg atgtgctggg
     1381 ctggggcggc tactgggcct gggatccggt ggaaatcgcc gcgtttatgc cctggctgac
     1441 cgggacagcc ttcctgcact cggtgatgat ccaggaaaag cgcggcatgc tcaagcactg
     1501 gaatatgatc ctgatcatct tgacttactc gctggtgatc tttgggacct tcctgacccg
     1561 ctcgggtgtg ctttcatcgg tgcatgcgtt cgcccagagc gccatcggtc cgctgttctt
     1621 tgcttttatc gggattactt ttattatctc gctggcgctg ctcctgcacc gctgggaaag
     1681 cctgaaatcc gagtcgcata tgacctcgct tttgtcacgc gaggcgctgt ttttgttgaa
     1741 taacctcctg ttcctgggga ttttggtgat ctgcttctgg ggagtgatct tcccgcttat
     1801 ctcagagctg gtcaccgggc agaaagtcac ggtaggaccg ccattctacg agcgcgcgac
     1861 cggtcccttg tttgcgggac tgcttctgct gatgggtgtc gcgcctttag ctgcctggcg
     1921 gcattcaact ggcaaaacgc tgggtaaagc gatctggaaa ccaaccatct tcgctgtgtt
     1981 ggtcacggca gtcctggtcg cgctgggcgt ccgcagcctg cctgcactgc tgactttctt
     2041 actctgcgcc cttgtgctgt cggtaactct gtatgaattc tggcgcgggg cgtgggcccg
     2101 gcaccgcacc tcaggtgaaa gcctgcctgt ggcgctttgg aatctggcgg ggcgcaaccg
     2161 ccggcgctac gggggttata ttatccatat cgggattgta ttgatggcag tggggatcat
     2221 cgggatcgag gtcttccaga cggaaacgca ggggacggtg ccagagggcg gtgagctgca
     2281 gcttggcgaa tacacaatgc gctttgactc actggcggtg ttcgatacag acgatcgcaa
     2341 tgtcgcccgt gccgtggtga gtgtatttaa agatggcgat tatgtgggtg aatattaccc
     2401 gcggcgtgac tactactatg aagctcagca gtcgatgacg atccctggcg tgcgcagcac
     2461 gctggaagat gacttctatg ttttactggt tgattgggaa gcaatctcca gcaatggggt
     2521 tacttttaag gtcttccata atccactgat caactggctg tggctgggcg gcctcgtgtt
     2581 tattctggga acgatggtcg ctgcctggcc ggataaagac ccggagccag ctgccgtccg
     2641 cactcgccgg cgaagttatg ccccgataga ggcctgagcg gggaggataa caggttgaac
     2701 attctggcag cagatcggaa ctctgtatga aacgaacggc tctggttgtc tttcaggtaa
     2761 ttatactgct ggctttcggc agcagcctgg cgctggcggc ggagccggct ccctggctgc
     2821 aggatggcgt cccgcgccag ccaaccgatg atgaggtgaa cgcggttgcc aagcagctgt
     2881 actgtccggt gtgcgagaac attccgctcg atgtttgtcc tactctggca tgcgagcagt
     2941 ggcgcagcct gatccgcgag aaacttgcgg ctggctggag cgaggaagag atcaagactt
     3001 actttgtcaa tcagtatggc gaccgggtcc tggcgacgcc ccccacccgg ggcttgaact
     3061 ggctggtgta catcattcct ccagcagcca tcctggcagg cgcttttatt ttgttccggg
     3121 cacttcgcgc ctggagaaag ccggtcgaag gagttgagtc agagactcca gcgctggcag
     3181 aggctggcat acccgatgag tacaccgccc ggctggaaga cgaacttcgc aagcatcagg
     3241 ctggcagcag cactgtcaag agagacgaat tatgatgaat gaacaggtag aatcaacccc
     3301 cgaggtgggg agtgcccgcc cgcgcctgcg ttgggggcgc atcctggtct ggactggctt
     3361 gctcggtctg ctggcattga tggcaatggg cttattgaac acccagcgcg gcccggtgct
     3421 gatcggacaa aatgctcctc aattcgtcct gtccaccttt gatgggcggg aaattaacac
     3481 ccaggatctg gcaggaaaag tggtcgtggt gaatttctgg gcctcgtggt gtaagccatg
     3541 tgagcaagaa gctgccgatc tggaagctgc ctggcagatt tatgccccaa ccgaacaagt
     3601 tgtcttcctc ggtgtggatt atgtggatac cgaacccgaa gcgctgggtt acctgcagaa
     3661 atttaatatc acctatgcca atggaccgga tctgcgcact cggatctcgc aggcctaccg
     3721 cattcgcggt gttccagaaa cctattttat agaccgccag ggaaagctgg ctgacgtcca
     3781 gattggtccg ttcagcaacc tggcccaaat caccggcaaa attgagccac tgctggcccc
     3841 ctgagcggcc cggtggttta aataatctta aaagactgaa agatactctg catggatatt
     3901 ggctcgatat taatcatcct ggcattatta atcccggttg tcctgtttat cgcccgcccg
     3961 ttttacgaac ctcgcctgat tgaacaggaa ggtttgaacc agcacgaaga ccatgatctg
     4021 tccggcttgc tggccgagcg cgaccgcatc ctgaatgcct tacaggagct ggaattcgat
     4081 catgtcctgg gcaaaatccc ggatgaagat tacccggcgc agcgcgcctg gttagtacag
     4141 caaggtgcgg atgtcctgcg caggctggat caaattcagg cttcggatag tctgcttgca
     4201 ggaaagccag aagg
//
LOCUS       DATMXK010000247         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_47170, whole genome shotgun sequence.
ACCESSION   DATMXK010000247 DATMXK010000000
VERSION     DATMXK010000247.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47170"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2030
                     /locus_tag="VN363_04585"
     CDS             <1..>2030
                     /locus_tag="VN363_04585"
                     /inference="COORDINATES: protein motif:HMM:NF037660.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83819.1"
                     /translation="LARLRARGQTVELRASDLRFTESEAAQFLNELMGLNLDHGSIAT
                     LAARTEGWIAGLQMAALSMRDRRDGRGFIQGFSGTNRFILDYLLEEVLASQPPEIQHF
                     LLHTSILERMNAPLCDAVLASSPERQPEAGDQAPAPPSNLSASVLAYLEHANLFLVPL
                     DDEQIWFRYHHLFGDLLRARLQQLEPDQPPRLHIRAAAWLEQNGLVPEAIHHLCAAGE
                     LDQAAALIERDGPARLADSDPSVIQLADSLPQSMILARPVIGLYQVWMLILHARIPEA
                     IPLLKSLAAQLADPAFKTGPPWMQAVIATALAFLAPEHNHANYPLPELNLIEAIPPGE
                     RILRNAADYLYGMALARRAGIARGIEFALQSIQREQLISTTLAQAAGPGAPANPAAPM
                     IPTLAPMLTRMYLMVGRLHAAAALCRQFLDPIDSHASRFIYTAGSMKIDLGEVLYEWN
                     RLEEAEAMVREGLQDNLLFRNIMTDGFGLVALTRILQARGDHAGALQACEQFETRLRA
                     SAQPREFDEALRSLKVRLQIAAGDLDRATQWAEQIMLSEDFHLHPEFYRSAMARLRLA
                     QGRYAEVAEILEQIPPPFAVTSRLSMQLESILLLAISAAAQDRHPEAYQLIASALDLA
                     EPEDCIQVFLDAGEPLHGLLAGYLRSPAPQHAEFVRKILLAYPQPAWLGHAWP"
BASE COUNT          348 a          715 c          563 g          404 t
ORIGIN      
        1 ctggcccgcc tgcgtgcccg cggccagacc gtggagctgc gcgcatctga tctgcgtttt
       61 accgaaagcg aagccgccca atttctgaat gagctcatgg gactcaactt ggatcacggc
      121 tcgattgcca cccttgccgc ccgcaccgaa ggctggatcg ccggcctgca gatggcagcc
      181 ctctccatgc gcgaccgccg cgatgggcgc ggttttatcc agggcttttc cggcaccaac
      241 cgcttcatcc tggattacct gctcgaggag gtcctggcca gccagccgcc cgagatccag
      301 cattttctgc tccacacctc gatcctggag cggatgaatg cccccctgtg tgatgcagtt
      361 ctcgccagca gcccggagag gcagcccgag gctggtgacc aggcaccagc tccaccctcc
      421 aacctatctg cgtccgttct ggcctacctg gagcatgcca acctcttcct ggtgccgctg
      481 gatgatgagc aaatctggtt ccgctaccac cacctgtttg gcgatctgct gcgtgcccgg
      541 ctgcagcagc tcgagcccga ccagccgccc cgcctgcaca tccgggctgc cgcctggctg
      601 gagcaaaatg ggctggtccc cgaagcgatc caccatctct gcgccgccgg cgagctggac
      661 caggctgccg ccctgatcga gcgcgacggc cctgcccggc tggctgacag cgatccttcc
      721 gttatccagc tggccgacag ccttccccaa tccatgatcc tcgcccggcc ggtcatcggt
      781 ctctaccagg tctggatgct catcctgcat gcccgcattc ccgaagccat tcccctgctg
      841 aaaagtctgg ccgcccagct ggcggatccc gccttcaaaa ccggcccgcc ctggatgcag
      901 gccgtgatcg ccactgccct ggcttttctt gccccagaac acaatcatgc gaattatccg
      961 ctcccggagc tcaacctgat cgaagcgatc cctcccgggg agagaatctt gcgcaatgct
     1021 gccgattacc tgtacgggat ggccctcgcc cggcgcgccg gcatcgcgcg gggcatagaa
     1081 tttgccctcc agtccatcca gcgcgaacag cttatttcca ctacgctcgc ccaggctgcg
     1141 gggccaggtg cgccagcgaa tccggccgct ccaatgattc ccaccctggc gcccatgctg
     1201 actcgcatgt atctgatggt gggtcgcttg cacgccgctg ccgccctctg ccgccagttt
     1261 ctggatccca tcgattccca cgcctcccgc tttatctaca cggccggcag tatgaagatc
     1321 gacctggggg aagtgctcta tgagtggaac cgcctggaag aagccgaggc tatggtccgt
     1381 gaaggtctgc aggataattt actttttcgc aatattatga ccgatggctt cgggctggtt
     1441 gccctgaccc gcatcctcca ggccagggga gaccatgccg gggcgcttca agcctgtgag
     1501 cagttcgaaa ccaggctgcg cgccagcgcc cagccgcgtg agttcgacga agctttacgc
     1561 agtctcaaag tccgcctgca gattgccgct ggagatctcg accgcgcgac gcagtgggcg
     1621 gagcagatta tgctcagtga agatttccat cttcatcctg aattttaccg ctccgccatg
     1681 gcccgcctgc gcctggcgca ggggcggtac gctgaagtcg ccgaaatcct ggagcagata
     1741 cccccgccat tcgcggtaac cagccggctg agcatgcagc tggaatccat tttgctgctt
     1801 gccatttcag ccgccgcgca agaccgccac ccggaagctt atcaattaat cgcctccgcc
     1861 ctggacctgg cagagccgga agattgtatc caggtctttt tagacgccgg cgagcctctc
     1921 catggcctgc tggccggcta tctccgttca ccggccccac agcacgctga atttgtccgg
     1981 aaaatcctgt tggcttaccc gcagcccgcc tggcttgggc acgcctggcc
//
LOCUS       DATMXK010000248         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_105766, whole genome shotgun sequence.
ACCESSION   DATMXK010000248 DATMXK010000000
VERSION     DATMXK010000248.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105766"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..389)
                     /locus_tag="VN363_04590"
     CDS             complement(<1..389)
                     /locus_tag="VN363_04590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83820.1"
                     /translation="MSAANPIQHTNRAAYLQNRILKPLLSHLVMIGVVLFIMFPMVYA
                     ILLSTQTPAEYYRMQLVPGSSFLSNMREAWDSVNMGQLLLNTSKVALVVATGKIILSI
                     IGAFAFVYFEFKGKTLIFLLILVTHMLP"
     gene            complement(386..1270)
                     /locus_tag="VN363_04595"
     CDS             complement(386..1270)
                     /locus_tag="VN363_04595"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter permease"
                     /protein_id="HWQ83821.1"
                     /translation="MNQTRFREPVLPYVLLAPMVVIVIIFFLYPSAQSLYLSVFRVSP
                     LGDRKIFVGLENFTRLLASKDYLNSLIVSVKFAGIVVLGGLSASLFSAVVANQRLRGF
                     AVYRALMIWPYALSPAIAGLLWGLMMNPNFGLGSVLLKRFTGIEFNYLTNGTSALLFL
                     ALAAIWKMFGYNIVFFLAGLQGLPTELLEAASLDGAGAWTKFWRITFPLLSPTTFFLF
                     VMNTLYAFFEVFGLVHITTGGGPGQATNLLVFNLYKDGFIAMNSGYASAQSILLLVMV
                     AALTLLQFRFAGEKVHYQ"
     gene            complement(1398..2597)
                     /locus_tag="VN363_04600"
                     /pseudo
     CDS             complement(1398..2597)
                     /locus_tag="VN363_04600"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="internal stop; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
BASE COUNT          548 a          758 c          788 g          503 t
ORIGIN      
        1 ggcaacatgt gggtcaccag gatcagcaaa aagatcagcg ttttgccctt gaattcgaag
       61 tacacgaagg cgaacgcccc gataatcgac aggatgatct ttccggtcgc caccaccagc
      121 gccaccttgc tcgtgttcag cagcagctgg cccatgttca ctgagtccca ggcttcgcgc
      181 atattgctca aaaagctgga gcccggcacg agctgcatgc ggtagtattc ggcgggggtc
      241 tgcgtgctga gcaggatggc gtaaaccatg gggaacatga tgaacagcac caccccgatc
      301 atcaccaggt gggagagcag cggcttgagg atccggttct gcaggtacgc cgctcgattg
      361 gtatgctgga tcggattggc ggcgctcatt ggtaatgcac cttttctccg gcgaagcgga
      421 actgcagcag ggtcagcgct gccaccatca ccagcagcag gatcgactgg gcggaagcat
      481 agccgctgtt catggcgata aagccgtcct tatacaggtt gaacaccagc agattggttg
      541 cctggcccgg tcctccgccg gtggtgatat gcaccagccc gaagacctcg aagaaggcgt
      601 acagggtgtt catcacgaac aggaaaaagg tggtcggcga gagcagcggg aaagtgatcc
      661 gccagaactt ggtccatgcc ccggcgccat ccagggaggc ggcttcgagc agctcggtgg
      721 gcagcccctg caggccggcc aggaagaaga cgatattgta gccaaacatc ttccagatcg
      781 ccgccagcgc caggaacagc agggccgagg taccgttggt caggtagttg aactcgatcc
      841 cggtgaagcg cttgagcagc accgaaccca gcccaaaatt ggggttcatc atcagccccc
      901 acagcagccc ggcgatggcg ggcgagagag cgtacggcca gatcatcagc gcccggtata
      961 ccgcaaagcc ccgcaggcgc tggttggcga ccactgccga gaacaggctg gcgctcaatc
     1021 cccccagcac cacgattccg gcaaatttca cgctcacgat cagcgaattc aggtaatcct
     1081 tactggcgag caggcgggtg aagttctcca gcccgacgaa gatcttgcgg tcgcccaggg
     1141 ggctgacccg gaaaacgctt aaatacaggc tctgggcgct gggataaaga aaaaagataa
     1201 tgacgataac caccatgggc gccaggagca cataaggaag taccggttca cggaatcggg
     1261 tttgattcat ttattcattt tccgaacacc ttcaaacccc aggggtttaa gaaatccgca
     1321 ggatttcaac ccctggggtt tagtgggttt accgaaggcg agagtaacaa ttcatccggt
     1381 gggtcgaaaa ggatctgcta ctgctgcagc gagatgtaat ccgccagcac ctggttggta
     1441 tcggcggtcg ccttatccag cgccgtcttg ggatcgacct tgttcacgac cacatcttcg
     1501 atggccgcgc ccacgatatc ccggatcgtg acgaagtcac ccagcatcac accgttggag
     1561 gcaatcgtgt ccgtcccgcc caggatctgg ttgaaagcgg tggcgtggtt gggttcttcc
     1621 tcgaaccagc cctcagcctt gagcttctca taagcgctga cgctggtggg gaagtagccg
     1681 gtgcccttgt gccaggcaat ggcttcatcg tcagagaagg tgaacttgaa gaactcccag
     1741 gcggcggcct gctgctcggg ggtggcgctg tcaaataccc acaggcacgc cccgccgatc
     1801 acgctgttgc ccgccggata ctcgtcgctc aggcgcggca ggaaagccgt gccgactttg
     1861 aaggtcgcgt tcttgaggat gccgcccagg ctggaggtgg actggatcag gaaggggaac
     1921 tgccccgcca ggaagggatc attggcggcg tattcacgcc cgccgtacac caggatgcca
     1981 tcgccggcca tttcctgcca tttagtcatc atctccaacc cgaaatcgga gttcaggtac
     2041 acttcgctcg ccagggcgct gcgcccattg tcgttgttgg caagcagctg gtcgtggtag
     2101 gcgaacatct gctcaaagat ccacgccggc cagcccatgg agaagccgcc cgtcgccagg
     2161 ccggcatcca tgatggtctt ggacatgtcc tcgatatcgt cccaggtcac cggggccact
     2221 tccgggtcca gcccggcggc ggtgaacata tccttgtttt aatagatcat ggcggtggag
     2281 ctgttgaagg gcatgcaggg cagattgcct tccagggtgt agtacttgcg gatcggggtg
     2341 accacttctt cccagtccac atcgcccttg ttcagggtgt ataccggcac gatggcgccc
     2401 gaatcataca tggtgcgggt gcccacttcg taggactgta cgatggtcgg tgcggcgccg
     2461 gagcggtagg ctgccaggcc ggcagtcacc gtctcggcat agctgccttt gaattccggc
     2521 ttcaccacga tcccgggatt ggcggcgttg aagcgctcgg cgatgccgtt gacgatctct
     2581 tcccggctgc cgctcat
//
LOCUS       DATMXK010000249         2698 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_34484, whole genome shotgun sequence.
ACCESSION   DATMXK010000249 DATMXK010000000
VERSION     DATMXK010000249.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2698)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2698)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2698
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_34484"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2698
                     /locus_tag="VN363_04605"
     CDS             <1..>2698
                     /locus_tag="VN363_04605"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ83822.1"
                     /translation="LRSQLDWAIYRRTADLKRVNSDLEKENEGRKQLETVISRGKREW
                     EGTFDSVPDLIVVTDTEGRIIRCNRATSTALKSGFRELIGRPVEGLFFGEQGEVQVQM
                     PAQAAEMRFPQLPGWYEVTSSELILEGGRKGTIYILRNITDRIQASLDLQRQKQYYES
                     LVKNSPIAIVTLDLEHRIVACNPAFEKLFGFSQIEVIGHDLDSLVSVDELKEETQAST
                     QSVRMGKVVHYFTRRRRRDGTILDVEMFGVPVVVMGKQIGILALFNDITELTRARREA
                     ENADRSKSAFLANMSHEIRTPMNGVIGMLELALDTPLTDEQEDYLKTSLESAESLLAL
                     LNDILDLSKIEARKLDLEMIDFNLHSTVEDVAHILAQRAQVKNLELTSFIQPEVPAHL
                     RGDPGRLRQVLVNLVGNAIKFTNQGEVELRTGLIHDDGQYALLRFEVKDTGIGISPEN
                     HAALFQTFSQADVSITRKYGGSGLGLAISKQLVELMGGQIDFESEPGKGSTFWFTAVF
                     EKQTAPEEQNIAVPMDLQDLHVLVVDDNATNRSVLTKLVTGFGCRVEAAESGLAGIRL
                     LREARSLNDPYKVLLLDMQMPDLDGQHTAVQIKADPEIEAVKILILTSIGQRGDAARF
                     IELGCDGYLLKPIKQQQLLEALLAVVGRLAAAAPKRRKLITRHTISEQQKERPPILLA
                     DDNSVNRKLVTTLLEKAGYRVDSVTTGSQAIEAVQQKQYGLVLMDGQMPEMDGFEAAQ
                     KIRLLESDARSTPIIAMTAYAMKGDRDLFLEAGMDDYIPKPLEPELLFAIIEKWIWDR
                     PRQVDREEPGAGAPEHVLTADLTQTIETEPEKDAEVQTPSSDDLPAWLPEELLFSGLN
                     SDSDTDEVTNWINTHVPPYNEALAGEIELPTDEVDNFDEGGII"
BASE COUNT          688 a          658 c          754 g          598 t
ORIGIN      
        1 ttgcgcagcc agcttgattg ggcgatttac cggaggacag ccgatctcaa gcgcgtcaac
       61 agcgatcttg agaaagaaaa cgaaggtcgc aaacagctcg aaacggttat cagccgcgga
      121 aaacgagagt gggaaggcac attcgattca gtccctgatt tgattgtggt taccgatacc
      181 gagggcagga taattcgctg caatcgggcc accagtactg cactgaagtc cggtttccgg
      241 gaattgatcg gcaggcctgt ggaaggatta tttttcggcg aacagggcga agtccaggtt
      301 caaatgccag cccaggcagc agagatgcgc ttcccgcagc tgcctggatg gtacgaggtg
      361 accagcagtg aattaatcct ggaaggaggc cgcaaaggga ccatctatat tttacggaat
      421 atcaccgacc ggatccaggc ctcccttgat ctgcagcgtc aaaagcaata ttatgaatcc
      481 ctggtcaaaa acagcccgat cgcgatcgtt accctcgatc ttgaacaccg catcgtcgcc
      541 tgtaatccag cttttgagaa attattcggt ttctcacaga tcgaagtgat tgggcacgat
      601 cttgacagcc tggtttcggt ggatgaatta aaggaagaga cacaggcaag cacgcagtcg
      661 gttcggatgg ggaaagtcgt tcattatttc acccggcgcc gccggcgcga tggtacgatc
      721 ctggatgtcg agatgttcgg cgtgcccgtc gtggtgatgg gaaaacagat cgggattctg
      781 gcgttattta acgatattac cgagctgacc agggcgcggc gagaagccga gaatgccgac
      841 cggtcaaaaa gcgccttcct ggcaaatatg agccatgaaa tccgcacccc gatgaatggg
      901 gttatcggca tgctcgagct ggctctggat acacctctga ccgacgaaca ggaagattat
      961 ctgaaaacgt cactggaaag cgcagagtcc cttctggcac ttttaaacga tatccttgac
     1021 ctttcgaaaa tcgaagcccg caagcttgac ctggagatga tcgatttcaa ccttcacagc
     1081 acggtagagg atgtcgctca tatactcgcc cagcgggccc aggtaaaaaa tctggagctg
     1141 accagcttca tccagcctga ggtaccggct cacttacgcg gcgacccggg caggctgcgc
     1201 caggtgctgg taaatctcgt cgggaatgcg attaagttca ccaatcaggg tgaagtagag
     1261 ctgcggaccg gtttgatcca cgatgatggc caatatgcac ttctcaggtt tgaggtgaag
     1321 gatactggga ttggtatctc acccgaaaac cacgccgcgt tgtttcagac attctcacag
     1381 gcagatgtat ccatcacccg caaatatggt ggttctggtt taggtctggc gatctcgaag
     1441 cagctggttg agttaatggg cggccagatc gacttcgaaa gtgagcctgg caagggcagc
     1501 accttctggt ttacagccgt tttcgaaaag caaaccgctc ctgaagaaca gaacattgcc
     1561 gtgccgatgg atcttcagga tctacatgtt ctggtcgtcg acgataatgc caccaaccgg
     1621 tcggtgctga ccaagctggt cactggtttc ggatgccggg ttgaagcagc tgagagcggt
     1681 ctggctggga ttagactttt acgtgaagcc aggtctctaa acgatcctta caaggtgctg
     1741 ctcctggata tgcagatgcc agatctggat gggcagcata ctgcggtcca aatcaaagct
     1801 gatcctgaaa tagaggcagt gaagattctt atactgactt ccatcgggca acggggtgat
     1861 gcagcccgat ttattgagct gggctgtgat ggatatttat taaaaccgat caagcagcag
     1921 cagcttctgg aagctttact ggctgtcgtt ggccggctgg ccgccgcggc tcctaaacgt
     1981 cggaaattga tcacccgcca taccatctcc gagcagcaga aagaacgccc accgatcctg
     2041 ttagcagatg ataattccgt caatcgcaag ctggtgacaa ccttactgga aaaggctggt
     2101 tacagagtcg attcggtaac caccggtagt caggcgatcg aagcggtcca gcagaaacag
     2161 tatggactgg tgctgatgga tggacagatg ccagagatgg atggctttga agctgctcaa
     2221 aagatccgcc ttctggagag cgatgcccga tcgacgccta ttatcgctat gactgcttac
     2281 gctatgaaag gggatcgcga cctcttcctc gaagcgggta tggatgatta cattcccaaa
     2341 ccgcttgaac cggagttatt gtttgcgatc attgaaaaat ggatctggga ccggccgcga
     2401 caggtcgacc gggaagaacc tggcgccggt gcgccagagc atgttttaac ggctgacctg
     2461 acacagacca tagagactga accggaaaag gatgctgagg tacaaactcc atcaagcgat
     2521 gatctgccgg catggctgcc cgaggagctt ttattttcgg gattaaattc tgactcagac
     2581 acagatgaag taactaactg gatcaacacc cacgtgccac catataatga ggctctggct
     2641 ggcgaaattg agctgccaac ggatgaggtc gataatttcg atgagggagg aataattt
//
LOCUS       DATMXK010000250         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_115971, whole genome shotgun sequence.
ACCESSION   DATMXK010000250 DATMXK010000000
VERSION     DATMXK010000250.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115971"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2236)
                     /locus_tag="VN363_04610"
     CDS             complement(<1..>2236)
                     /locus_tag="VN363_04610"
                     /inference="COORDINATES: protein motif:HMM:NF013618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ83823.1"
                     /translation="PTPIPAARIDLAEKALFNGDWDRAFQEFETARLASSDPDLQAAA
                     LLGLGRTYYLDAKYEQAATVLTSLIQQFPDGVDAAYAYLILGQIYTALGQPANAAQAY
                     LDYMVRRPGVIDGYVLDLRADALFAAGDYSGSANDYKAALQSPTASDGIFIEMKLARA
                     IALSGDASGAIALYADIYARTNNESTKALSLLRRGQAYMSLGQGELAIESWMAAVNLH
                     PRTNEAYQALVELVDAGQVVNELQRGIVDYYAGQYGVALAAIDRYLQSGPTDPGTAHY
                     FNGLINRALGGNATAINEFDVVLQNFSENAYWAEALNQKAYTQWAFMDQYTQAIQTLR
                     DFVNLYPTQARASEYLFDAAQVANTAGQLELAAELYLQVSASYPNSEEALRAMFLAGI
                     TYYRLGNFAQAFTTFQNYLGSAAQLGDRAAAYLWMGKSLIAQGDQAGARTQWELAANT
                     DPTGYYSERARDLLVNRPAFTPPQMYDLVFDPNAERQQAETWLRTQFSIPDSIDVTEP
                     GELAGEAGMIRGNELWALGLFNEARNEFEAIRQAYTQDPIQTYRLMNYFYDLGLYRSA
                     IFAARQVLNLANMSDADTMSAPPYFNHVRFGSYFSELIIPSSQEYGLHPLMVFSLVRQ
                     ESLFEGFIQSSAAASGLMQIIPATGEEIAARLNWPEDYTQPDLYRPYVNVRFGVEYLD
                     RQRNYFKGDLYAALAAYNGGPGNAIRWNSLAAGDQDLFLEVVSFEETRTYIQRIYEIF
                     TIYR"
BASE COUNT          513 a          614 c          627 g          482 t
ORIGIN      
        1 ctgcggtaga tcgtaaaaat ttcgtaaatc cgctggatat aggtgcgagt ttcctcgaaa
       61 gaaacgacct ccaggaaaag atcctggtca ccagccgcca ggctgttcca gcggatggca
      121 ttaccagggc cgccgttata cgctgccagc gcggcataca ggtcgccctt aaaataattc
      181 cgctggcgat cgagatattc gacaccaaaa cgtacattga catacgggcg gtacaggtct
      241 ggctgggtat aatcctcagg ccaattcaga cgcgcggcta tctcttcccc ggttgcgggt
      301 atgatctgca tcaggccgct ggcagccgcc gaggactgga taaaaccttc gaacagactt
      361 tcctgccgca caaggctgaa taccatcagg ggatggagtc catattcctg ggaagatgga
      421 atgatcaact cggaaaaata gcttccaaag cggacatgat tgaaataagg cggtgctgac
      481 atcgtatcgg catcgctcat gttggccagg ttcaacacct ggcgggcagc aaagatcgcc
      541 gagcggtaca gacccagatc gtaaaaataa ttcatcagcc ggtaagtctg gattggatcc
      601 tgggtatacg cctggcggat ggcttcaaat tcgttccggg cttcattaaa taaacccaga
      661 gcccacagct cattaccgcg gatcattcct gcctcgccag ctaattcgcc aggctcggtt
      721 acatcgatgg aatcaggaat tgagaactgg gtgcgcagcc aggtttcagc ctgctggcgt
      781 tcggcattgg ggtcaaatac caggtcgtac atctggggcg gggtgaacgc cgggcgattt
      841 accaacaggt cacgggcacg ctcgctgtaa tagccggtgg gatcggtatt ggcagccagc
      901 tcccattgag tgcgggctcc ggcctgatcg ccctgggcga tcagcgattt acccatccag
      961 agataagctg ctgcacgatc tcctaattga gctgccgatc ccaggtaatt ctggaaggtt
     1021 gtaaaagcct gggcaaaatt ccccaggcgg taataggtaa tcccggccag aaacatggcc
     1081 cgcaaggctt cttctgagtt gggataggac gcgctgacct gtaagtagag ctcagcagcc
     1141 agttcgagct ggccagcggt gttggccacc tgagcagcgt caaacaggta ttcagaggcg
     1201 cgcgcctggg tagggtaaag gttcacgaaa tcgcgcaggg tctggatagc ctgggtgtac
     1261 tggtccatga atgcccactg cgtgtaggct ttctggttca gggcttctgc ccagtatgcg
     1321 ttttcggaga aattttgtag aaccacgtca aactcgttta tggcggtggc attgcctccc
     1381 agagcgcggt tgatcaaccc attgaaataa tgtgcagtac ccggatcagt aggacctgat
     1441 tggagatagc ggtcaattgc cgccagtgcc accccatact gcccggcgta ataatccacg
     1501 atcccgcgtt gcaattcatt gactacctga ccggcatcga ccagctcaac cagggcttga
     1561 tacgcctcgt tggtgcgcgg gtgcaaattg accgctgcca tccagctctc aatcgccagc
     1621 tctccctgac ccagcgacat gtaagcctga ccccggcgca gcaaagacag tgctttggtg
     1681 ctctcattat tcgtccgggc atagatatcg gcatacaggg cgatcgcgcc gctggcatct
     1741 cctgaaaggg cgatcgcccg ggctaatttc atttcaatga atatcccatc gctggcagtt
     1801 ggcgattgga gcgcagcctt gtaatcattg gctgagcctg aataatcacc tgctgcaaac
     1861 aaggcatcgg cccgcagatc cagaacatac ccgtcgatca cccctggacg acggaccatg
     1921 tagtccagat acgcctgggc agcgttggcc ggctggccca gggcggtata gatttggccc
     1981 aggatcaggt aggcgtaggc agcatccacc ccatctggga attgctggat aagactggtt
     2041 aaaacagttg ctgcctgctc gtatttggca tccaggtaat atgtccgtcc cagtcccagc
     2101 aaagcagccg cctgcaggtc gggatcgctg ctcgccaggc gtgcggtctc aaattcctgg
     2161 aaagcccgat cccaatcccc attgaagaga gctttttcag ccaggtcaat ccgtgccgcc
     2221 gggatcggtg taggcg
//
LOCUS       DATMXK010000251         5929 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_84590, whole genome shotgun sequence.
ACCESSION   DATMXK010000251 DATMXK010000000
VERSION     DATMXK010000251.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5929)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5929)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5929
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84590"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..856)
                     /locus_tag="VN363_04615"
     CDS             complement(<1..856)
                     /locus_tag="VN363_04615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83824.1"
                     /translation="MPTSFSDFSSFLIFFIYGLAFFSMGLILLIESSRASQIEGVRLL
                     LPLALFGFIHGIHEWIEMALMQPAWFGQLLPDYAVWLRLGILATSFMTLQTYVLRIFF
                     PRLEKYWVVGLTGLTALLLVLAVWVGAYWQSLPADWAVKADAAIRYFLAIPGALAAAL
                     VMLRQARQSTSPGDRSLATGWKLAGYSFAGYAFSQSVVRSSNFLLADVWNTSIFFDLT
                     GFPVQALRAGLAVLMTVGLILAVQAMEVERQQELLAAQMARLEALEQMQNEARARAAM
                     HKDLLRYTV"
     gene            1001..3478
                     /locus_tag="VN363_04620"
     CDS             1001..3478
                     /locus_tag="VN363_04620"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWQ83825.1"
                     /translation="MRIKIIASVLVGLLILALAGGWVWLTHDLPGLDELPQRLNAPSL
                     RITDRNGVLLYEALPETGGRHGVLPIESIPLALQQATIATEDHDFYTNPGLDWRGILR
                     AAWANLRGGGIVAGGSTLTQQVARNLLLDESERYEQSLRRKLREAILAWRITRQLEKD
                     AILGLYLNQTYYGGMAYGVEAAAQTFFGKPAAELDLAECALLAGLPQAPSLYNPLSDP
                     EAAAERQRQVLGRMEAEGYIDAAQRSLAEREQLIFASTPYPIEAPHFVWMVRDELSSL
                     ISSETMYQSGGLIVRTTLDLSWQKSAESAILRQLAALQRSEDSLGHNVHNAALVAIDP
                     HSGEIKALVGSPDFFDASAAGAINMALAPRQPGSALKPLVYAAALDPSQPNPWTAATV
                     LLDVRTSFVTRDGLAYIPENYDRQEHGPVLLRDALASSLNIPAVITLEHVGLERLFAL
                     SEDLGIHTFRDPAQYDLSLALGGGEIRLLELTAAYGALATGGLRVYPQAILEVRDFSG
                     NVLYTPPSQTQTRVLDERLAWLVSDILSDDEARRIGFSAHSVLNLDRPAAVKTGTTSN
                     FHDNWTIGYTPDLVVGVWAGNTDYRPMRNVDGLSGAAPIWHQFMRSVLKDSPPGQFVQ
                     PPGFEQVEVCSLSGGLPTPACPYRRLEWFIAGTQPQHEDTYYQQVWLDRRTGLLAGET
                     TPGEDRQLQTVLDIPPQAQTWARSQGFLLLGDLQIQAARTETGSSPTQAGLQMVSPAD
                     RSVYRLSKGLDPAAQRIRLAAASQADFTRLSLWLDGAEIASFGDSPPYETWIELTPGE
                     HEAWAEAALPDGSLLASPRITFMVEAQ"
     gene            complement(3500..>5929)
                     /locus_tag="VN363_04625"
     CDS             complement(3500..>5929)
                     /locus_tag="VN363_04625"
                     /inference="COORDINATES: protein motif:HMM:NF036531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004866 - endopeptidase inhibitor activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-2-macroglobulin family protein"
                     /protein_id="HWQ83826.1"
                     /translation="VDVVSSLPLLVRPQTPRFFVIGDQVILGAAVHNNTAENLQVDVS
                     LQAQGVTLLGEASQAVEIGAGQQAYVAWEALVEPTDADGSPISRVDLIFKAVGGGYSD
                     TTRPTFGPLEGQGLPVYRYEAHETVGTSGQVPAGGTQVEAINLPSSWQAGEPHSGSLI
                     VKVAPSLAAGMTDGLEYLKSYPYECVEQTVSRFLPNVVTTHALRAAGISDPELETGLK
                     EQVNVALPRLYNWQNADGGWGWWNNQKSDPLTSAYVVLGLLEARDAGYSVTQANLDKG
                     LAYLRRNLRPITRLEQPHLINRQAFLLYVLARAGEPDVSATVQMYDQRLELALYARAY
                     LAHTLYWIDPADTRLPTLLSDFNTTAITSATGTHWEEASQDVYNWNSDTRTTAIILSA
                     ISEIDSQNPLNANAVRWLMAHRTNGYWNSTQETAWTILALTRWMVASGELNADYRYAV
                     ALNSNQLGDGTANAGNLRQTSEIKVDVNELLLGEANRLAIARDEGPGSLYYTAHLYVA
                     LPVEEVQALDQGIVVSRSYYSLDDPQTPIQEANIGDLLLARLTIVAPNTLHYLLVEDP
                     WPAGLEGVDQSLNTSTQSVSPYQFNRDDFTRNGWGWWYFNHVEMRDEKLALSATELPP
                     GTYVYTYLVRASTAGEFRTIPPTAQEFYFPEVYGRGEGSLFTVRQAEPTANIPESETQ
                     VDCSQIEHLAIDSVEAQDIVDQFTQNFKEQNPTEFMGMTILQGVDRLGEWVLVQGSVS
                     GEGKDVIVAQQTEQGYQIAERYIITAPLASFEDGESRVPDYFLEKLPEAPDELFTCLD
                     QSWLLSVGYTP"
BASE COUNT         1201 a         1847 c         1704 g         1177 t
ORIGIN      
        1 gaactgtata gcggagtaag tctttatgca tggcggcccg cgctcgggct tcattctgca
       61 tctgctccag agcttcgagg cgcgccatct gcgccgccag cagctcttgc tggcgctcta
      121 cctccattgc ctgcacagcc aggatcaggc caaccgtcat cagcaccgcc aggccggcgc
      181 gtaaagcctg gaccgggaag ccggtgagat cgaaaaaaat ggaggtattc cagacgtctg
      241 ccaggaggaa attgctggag cggaccactg actgcgaaaa agcgtagccg gcaaagctgt
      301 atcctgccag tttccacccg gttgccagac tgcggtctcc tggggaagtc gactggcgag
      361 cctggcggag cataaccagc gctgccgcca atgcccctgg gatcgccaga aaatagcgga
      421 tcgcggcatc ggccttcaca gcccaatcgg ccggcagaga ctgccaatat gcacccaccc
      481 aaactgccag caccagtaac agggctgtca gaccggttaa tccaacaacc caatatttct
      541 ccaggcgcgg aaaaaagatc cgcagtacat aggtctggag ggtcatgaat gaggtcgcta
      601 agatacccag gcgcagccag acggcatagt ccggcagcag ctggccaaac caggctggct
      661 gcatcagggc catttcgatc cattcatgga ttccgtggat aaaaccgaac agtgccagag
      721 gcagcagcaa gcgcactcct tcaatctggg aagcccggct ggattcgatc aataagatca
      781 gacccatgct gaaaaaggcc aggccataga taaaaaatat cagaaagctt gaaaaatccg
      841 aaaagctggt tggcatgctg aattcccgtg tggtggtggt taattctaac ctttttccgg
      901 gaacatctcc aagattggaa agtctattaa ccgtcaggtg ccgcgcagtc tgggtgcgtc
      961 tgtctctgat agaatcgact ttgataggaa agatatacag atgcggatca aaataatcgc
     1021 tagcgttctg gtcggactgc tcattcttgc cctggctggc ggatgggtct ggctcaccca
     1081 cgacctgccc ggcctggacg agctgccgca gcgcctgaat gcacccagcc tgcgcatcac
     1141 cgaccgcaat ggtgtcctgc tctacgaagc cctgccagag accggcgggc ggcacggcgt
     1201 gctgcctata gaatcgatcc cactggcttt gcagcaggcg actattgcca cggaagatca
     1261 tgatttctac accaatcccg gtctggactg gcggggcatc ctgcgcgccg cctgggccaa
     1321 cctgcgcggc ggtggtatcg tagcgggcgg cagcactctc acgcagcagg tggcccgcaa
     1381 ccttctgctt gatgaaagcg agcgttacga acaaagcctg cgccgcaagc ttcgcgaggc
     1441 gatcctggcc tggcggatta cccggcagct ggagaaagat gctattctgg gcctgtacct
     1501 gaaccagacg tattacggtg ggatggccta tggggtcgaa gccgccgccc aaacgttttt
     1561 cggcaaacca gccgcagagc tcgacctggc ggagtgcgcc ttgctggccg gtctgcccca
     1621 ggcgccttcc ctgtacaacc cattgagcga tcctgaagca gccgccgagc gccagcgcca
     1681 ggtgctgggg cggatggaag cggagggtta catcgatgcc gcccaacgca gtcttgcgga
     1741 acgcgaacag ttgatttttg cttcaacccc atatccgatc gaagcgccgc attttgtctg
     1801 gatggtgcga gatgagctgt ccagtctgat cagcagcgag accatgtacc aatccggcgg
     1861 actgatcgtc cgcactacgc ttgacctgag ctggcaaaaa agcgctgaat ctgccatcct
     1921 gcgccagctc gcagccctgc agcgcagtga ggacagcctc gggcacaatg tgcacaatgc
     1981 cgccctggtg gccatcgacc cgcacagcgg cgaaattaag gctctggtag gcagtcccga
     2041 cttttttgac gccagcgctg cgggggcaat caatatggct ctggcgcccc gccagcccgg
     2101 ctccgccctc aagccgctgg tctatgcagc cgccctggat ccatcccaac ccaacccctg
     2161 gaccgccgcg acggtcctgc ttgatgtacg caccagtttt gtcacccgcg acggcctggc
     2221 ctatatcccg gagaactatg accgtcagga acacggtccc gtgctgctgc gggatgcgct
     2281 ggcttcttcc ctaaatatcc cggctgtcat caccctggag catgtcggcc tggagcggct
     2341 gtttgccttg agcgaggacc tgggtatcca caccttccgc gatcctgccc aatacgatct
     2401 ctctctggcg ctgggcggcg gtgaaattcg cctgctcgag ctcacggctg cttatggcgc
     2461 cctggcgacc gggggtctgc gggtctaccc gcaggccatt ctggaggtac gcgacttctc
     2521 tggcaatgtg ctttacactc ccccatccca gacccagact cgcgtcctgg atgaacgcct
     2581 ggcctggctg gtgagcgaca tcctcagcga cgatgaggcc cgccggattg gctttagcgc
     2641 ccacagcgtc ctcaacctcg accgcccggc agccgtcaag accggcacca cctcgaattt
     2701 ccatgacaac tggacgatcg ggtacacccc cgatctggta gtgggcgtat gggctgggaa
     2761 tacagattac cgcccgatgc ggaatgtaga tggtctgagt ggggcagcgc cgatctggca
     2821 ccagttcatg cgcagcgtgc tcaaagattc tccccccggc caatttgtac agccacccgg
     2881 gtttgagcag gtggaggtat gcagcctttc aggaggcctg cccacacctg cctgccccta
     2941 ccgccgcctg gaatggttca tcgcggggac gcagccccag cacgaagata cttactacca
     3001 gcaggtctgg ctggacaggc gcaccggttt gctcgccggc gaaaccaccc ctggcgagga
     3061 ccgccagctg cagactgtac tggacatacc ccctcaagcc cagacctggg cgcgcagcca
     3121 gggcttcctc ctgctcggcg acttacaaat ccaggctgcc cggaccgaaa caggcagcag
     3181 cccgacccag gctggcctac aaatggtatc cccggccgat cgcagcgttt accggctgtc
     3241 aaaggggctg gaccctgccg cccaacgcat tcgcctggct gccgccagcc aggcagattt
     3301 cactcgcctg tcgctctggc tggatggagc ggaaattgcc agcttcggcg attctccgcc
     3361 ctacgagacc tggatcgagc tcacccccgg cgagcacgag gcctgggcgg aagctgctct
     3421 gccagatggc agtttgctag ccagccccag aataacattt atggttgaag cccaataata
     3481 acgcctctcc cacagctggt tatggggtgt aacccacact caacagccag gattggtcca
     3541 ggcaggtgaa caattcatcc ggggcctcgg gcagcttctc tagaaaatag tcgggaaccc
     3601 ggctctctcc gtcttcgaat gacgccaggg gagccgttat gatataccgt tccgcaatct
     3661 ggtatccctg ctcggtttgc tgcgcgacga taacatcttt cccctccccg gatacactgc
     3721 cctgcaccag aacccattcg cccaggcggt cgacgccctg caggattgtc atgcccataa
     3781 attcggtcgg gttttgctcc ttaaaatttt gggtaaattg atccacaata tcctgcgctt
     3841 ccacagaatc gatggcgaga tgctcgatct gggaacaatc gacttgagtc tcgctctccg
     3901 gaatattggc tgttggctca gcctgtcgga ctgtgaaaag gctgccctcg cctcttccat
     3961 atacctccgg gaagtaaaat tcctgggcgg tgggtgggat ggtgcgaaat tcacctgccg
     4021 tgctggctcg taccaggtaa gtatatacat acgtccccgg cggcagctcg gtggcgctca
     4081 aagccagttt ttcgtcccgc atctccacgt gattgaagta ccaccagccc cagccgttgc
     4141 gagtgaaatc atcccggttg aactggtaag gtgaaaccga ctgcgtgctg gtgttcaggg
     4201 attggtccac cccctccagc ccggctggcc agggatcctc cacgagcagg tagtgcaggg
     4261 tgttgggggc gacgatggtc agccgcgcca gcagcagatc ccctatgttg gcttcctgga
     4321 tcggggtctg cgggtcgtcc agcgagtagt agctgcggga gaccacgatc ccctgatcaa
     4381 gcgcctggac ctcttccacc ggcagtgcga catacaggtg agcggtatag tacaggctgc
     4441 ccggtccttc atcgcgggca atcgccaagc ggttggcctc acccaataac agctcattga
     4501 catcgacctt gatttcagag gtctggcgca ggttgccggc attcgccgta ccatctccca
     4561 gctggttact gttcaaagcg accgcataac gataatctgc attcagctcg cccgaagcga
     4621 ccatccagcg ggtcagcgcc aggatcgtcc aggcggtctc ctgagtgctg ttccagtagc
     4681 cgttggtgcg gtgcgccatc agccagcgca cagcattggc attcaatgga ttctgggagt
     4741 cgatctcgct gatcgccgag aggataatcg cagtcgtgcg cgtgtcgctg ttccagttat
     4801 agacatcctg gctggcttct tcccaatgcg ttccagtcgc cgaggtaatc gccgttgtat
     4861 tgaaatccga gagcagagtg ggcaggcggg tgtcagccgg atcgatccag tataaggtat
     4921 gcgccaggta cgcccgggca tacagggcca gttcaaggcg ctggtcgtac atctgcaccg
     4981 tggcgctcac atctggctcc ccggcgcggg ccagcacgta aagtaagaag gcctggcggt
     5041 tgatcaggtg gggctgctcc aggcgggtaa tcgggcgcag attgcgccgc aggtaagcca
     5101 accctttatc gagattcgcc tgggttacgc tgtagccagc atcccgggcc tctaacagac
     5161 ccagtaccac ataggcgctg gtcagcggat cgcttttctg gttgttccac cagccccagc
     5221 ctccatccgc attctgccag ttgtacaaac gcggcaaggc gacattgacc tgctctttaa
     5281 ggccggtttc cagctccgga tccgagattc cggctgctcg caaagcgtgg gtggtcacca
     5341 cattcggcag gaagcgggag accgtctgct cgacgcattc gtaagggtaa gatttcagat
     5401 attccagccc atcggtcatt cccgctgcca gggaaggcgc taccttcacg atcagcgagc
     5461 cgctgtgagg ctcgccagcc tgccagctgg aaggtaagtt gatcgcttcc acctgtgtgc
     5521 cgccggcggg tacctgcccg gaagtcccca cagtctcgtg agcttcatag cggtagaccg
     5581 gcagcccttg accttccaga gggccaaaag tcggtcgggt ggtgtccgag tagccgccgc
     5641 caaccgcttt gaagatcaaa tcaacgcggc tgattgggct gccatccgca tccgttggct
     5701 ccaccagcgc ttcccaggct acatatgctt gctgtccggc accaatttcc actgcctggg
     5761 aagcctcgcc cagcagggtt actccctggg cctgcaggct gacgtccacc tgcagattct
     5821 cagctgtatt attatgcacg gccgcgccca ggatcacctg gtcgccaatt acaaagaaac
     5881 gcggtgtctg cgggcggacc agcagcggca ggctgctgac cacatccac
//
LOCUS       DATMXK010000252         3173 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_48577, whole genome shotgun sequence.
ACCESSION   DATMXK010000252 DATMXK010000000
VERSION     DATMXK010000252.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3173)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3173)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3173
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_48577"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(86..976)
                     /locus_tag="VN363_04630"
     CDS             complement(86..976)
                     /locus_tag="VN363_04630"
                     /inference="COORDINATES: protein motif:HMM:NF013094.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ThiF family adenylyltransferase"
                     /protein_id="HWQ83827.1"
                     /translation="MFERNYGVFTAEEQARIRAAKVVIVGTGGIGGTVATILARSGVE
                     HFVLYEHDIYTITNMNRQIGCFTDTLGISKAEVITDSLLRINPEAQIQMESRSIVEAE
                     MDEVILQGDVFLPAADDWPLSMNMLGRAKELGKPAILAYPVGALGRVSIFMPSSPYAS
                     ECLVIPRGLPYAELEAFMRDPDNRRTLLYYCTEGGWTDEWFEGFCEGSKPHAQLCTIV
                     WITASLAAQEIIKLVSGRFDTVIAPRYWHVTPEKTYIARFSAARRLMARSSVKPWARA
                     LLPKIARRPWLLRLFTRALH"
     gene            complement(1120..>3173)
                     /locus_tag="VN363_04635"
     CDS             complement(1120..>3173)
                     /locus_tag="VN363_04635"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HWQ83828.1"
                     /translation="EEDPIMADLKGMTGKILRVDLTSGTTTVIEPEEEVYKKFLGGAA
                     LGVYYLLKEGIADPKVDPLGPDNMFQIMIGPITGVGPNNRSVTVTKSAYNFIGISTAG
                     GQTPSELKYAGWDGVQVVGKAEKPVYIAIMDDKVEIRDASHVWGKGAEEAEDILRAEV
                     NSEVETREAMLRDADLTPEWAAMRPPKRQGTGHKRLAKVWLIGPGGENQVWFASVMTE
                     AGRAHGRYGTGAIMGSKNLKAIVVRGTKGHPLADKKKYLELVYAIQSDELKNYSRKKY
                     GTASAGTRTSYTENAFPIRNWQWGVWSDPHMQGISGPFMDTASFIRKQACAGCTMNCL
                     FPVQITSEDPMMDGHASDMPDWEALGMVGGNLGYFEMDGDTPSDPFSGTLEDHAEHLA
                     KLQYTTYIHDDAGLDFIEGGALLAMLMELRQRDLISVDDLDGIDLKWGDVHAVDEIVK
                     KLVARDGIGDILATGTWETAKYFAEAKGKPEILKYAQVGHRYGQPAHGVRTGADKDAI
                     EYVTVSRPCEHTGGGGAGFIKGDLAAGIAGQNTKSAGSDSFVVCSFSAGNFAGKYVDM
                     LKAATGWTDFTDEDLVKVGARHYAMARLFDIHTQDLTDPKEQWDKLVTDRWFDDPMPN
                     GWAKGKVAYEGNKDKLFNETLPAYWKERGWTEDKGIPTLDTLKALGIDDVAGPIAEKH
                     L"
BASE COUNT          676 a          898 c          853 g          746 t
ORIGIN      
        1 caataatcct gctccaagca aagctacgat gttaatttca attttgctat tattcacaat
       61 cctcttcctt tcactctatc cgggcttaat gcagcgcccg tgtaaaaaga cgtaacagcc
      121 agggccgcct ggcaattttc ggaagtaatg cccgcgccca gggtttaacg gagctgcgag
      181 ccatcaggcg ccgggcagca ctgaagcggg cgatataagt cttttctgga gtgacgtgcc
      241 aatagcgtgg ggcaatcact gtatcaaatc gccctgagac gagcttgatg atctcctggg
      301 cagccagcga tgctgtgatc cacacaatcg tacacaactg ggcatgcggc ttgctgccct
      361 cacaaaaacc ttcgaaccat tcatccgtcc aaccaccttc agtgcagtaa tatagtaagg
      421 tgcggcgatt atccggatca cgcataaaag cttctaattc ggcgtagggc aagccccggg
      481 ggattaccag acattccgaa gcataagggc tggacggcat aaagatgctg actcgcccga
      541 gcgctcctac gggataagcc agaatagccg gcttgcccag ctctttggct ctccccagca
      601 tattcatact taaaggccaa tcatccgctg ctggcaggaa tacatctccc tgcaaaatga
      661 cctcgtccat ctcagcttca acgatcgaac gagattccat ttgaatttga gcttctggat
      721 tgatccggag aagagaatcc gtaattacct cggctttgct tatacccaaa gtgtcagtaa
      781 agcagccgat ctggcggttc atgtttgtta tggtatagat atcatgctcg tagaggacaa
      841 aatgctccac cccactgcgc gctaagattg tcgctacggt accacctatg ccccctgtgc
      901 caacgatgac gaccttagca gctcgaattc gcgcctgttc ctctgccgta aaaacgccat
      961 aattgcgctc aaacatctgc tcgtagttca cgaaaaagtc tctctctttc ctcaatgaaa
     1021 ttctagatca ccatggcgat gagtggcgag gccccaactt gatggattac acccagttga
     1081 ggcctcatcg atgcatctta atggttaccc gtcagcgatt tagagatgtt tttcggcgat
     1141 agggccggca acatcatcga tccctaaggc tttcagggta tcgagggtcg ggatgccctt
     1201 gtcctcagtc cagccgcgtt ccttccagta agccggcagg gtttcgttga agagcttatc
     1261 cttattgcct tcgtaagcca ctttaccctt ggcccagccg ttcggcatcg gatcatcgaa
     1321 ccagcgatcc gtaaccagtt tatcccattg ttctttggga tcggtcagat cctgggtgtg
     1381 gatgtcgaac aaacgtgcca tggcataatg gcgggcgccg actttgacca ggtcttcatc
     1441 ggtgaagtcg gtccagccgg tagcagcctt gagcatatcg acgtacttac cggcgaagtt
     1501 gcctgccgag aatgagcaga ccacgaacga gtcggagcca gcagacttgg tgttctgtcc
     1561 ggcaatgccg gcagccaggt cgcccttgat gaagcctgca ccgccgccgc cggtgtgttc
     1621 gcagggccgg ctgacggtga catactcaat cgcatctttg tcagcgccgg tgcggacgcc
     1681 gtgagcgggc tgtccataac ggtgaccgac ctgggcgtac ttcaagattt ccggtttgcc
     1741 tttcgcttct gcaaagtact tggcagtttc ccaggtgccg gtggccagga tatcgccaat
     1801 gccgtcacgg gcgacgagct tcttgacgat ttcatcaacg gcgtgaacat cgccccactt
     1861 caggtcgatg ccatccaggt cgtccacact gatcaggtcg cgctggcgca gctccatgag
     1921 catcgccagg agggcgccac cttcgatgaa gtccaggcca gcatcgtcat ggatgtaggt
     1981 ggtgtactgc agcttggcca ggtgttcggc atgatcctcg agggtaccgg agaaaggatc
     2041 agacggggta tcgccatcca tttcaaagta acccaggttg ccgccgacca tacccagggc
     2101 ttcccagtca ggcatatccg aagcgtgacc atccatcatt ggatcttcac tggtgatctg
     2161 gaccgggaac aggcagttca tggtgcagcc ggcgcaggcc tgcttgcgga tgaagctggc
     2221 ggtatccata aagggaccgc tgatgccctg catgtgcgga tcggaccaca caccccactg
     2281 ccagttgcgg atcgggaaag cgttctcggt gtaggaggta cgggtaccgg cagaggcagt
     2341 gccatatttc ttgcggctgt agttcttcag ctcgtcggac tgaatggcat aaaccagctc
     2401 gaggtacttt ttcttatctg ccaggggatg gcccttggtg ccgcgcacga caatggcttt
     2461 gaggttcttg gagcccatga tagcgccggt gccgtagcgc ccatgggcgc ggccggcctc
     2521 ggtcatgacg ctggcaaacc agacctggtt ttcaccgcca ggaccgatca accagacttt
     2581 tgccaggcgc ttgtggcccg tgccctgacg cttgggcggg cgcatggctg cccattctgg
     2641 ggtaaggtcc gcatcccgca gcatggcctc gcgggtttca acttccgaat tcacttcggc
     2701 gcgcaggatg tcttcggctt cttctgcacc tttgccccaa acgtggctgg catcgcggat
     2761 ctcaaccttg tcatccatga tggcgatata gactggtttt tcagccttgc cgactacctg
     2821 aacgccgtcc cagccggcat atttcagttc ggaaggagtc tgaccgccag cggtggaaat
     2881 gccgataaag ttgtaagccg atttggtgac ggtgacggag cgattgtttg gccccacacc
     2941 ggtgatcgga ccaatcatga tctggaacat gttgtccgga ccaagaggat ctactttggg
     3001 atcagcgatc ccttccttca agaggtaata gacccccaga gccgccccac ccaggaattt
     3061 cttgtagact tcttcctcgg gttcgatgac cgtggtggtg ccactggtca ggtcgactcg
     3121 taaaattttg cctgtcatac cttttaagtc ggccattata gggtcctcct ctg
//
LOCUS       DATMXK010000253         3569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12862, whole genome shotgun sequence.
ACCESSION   DATMXK010000253 DATMXK010000000
VERSION     DATMXK010000253.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3569
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12862"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VN363_04640"
     CDS             <1..987
                     /locus_tag="VN363_04640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83829.1"
                     /translation="AQASPTPPPTAASAQPASTSEPRVSSIATGKPAIIVDHTSVALF
                     EQIPEKYLAAARDLRMVYGDRSVGWNINQYLDCLAQPSWGDSPAFCRRDYLPGSDEFT
                     TYGPNRAVSGEAGESIRFTPDPVRYNRANWLFAEISDSWSDYTERFITEFAPGYLESM
                     DVLSYQFTYLHVSGDNGIADPATGYFADNPDSFDIVDLEAFIAQHPEKTFFFWTTSLA
                     RGIGTQEATDFNDQMRDYVGKNGLILFDVADIESHTPAGEPCYDNRDGVEFCTKNGNC
                     ENFPDDGVEFLALCREYTTELEGGHLGAVSGGGIQLAKAFWVLMAQIAGWQP"
     gene            complement(1029..2063)
                     /locus_tag="VN363_04645"
     CDS             complement(1029..2063)
                     /locus_tag="VN363_04645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ83830.1"
                     /translation="MEIRQVALVETEADPWGFSVPIMDAFKHAGYPCDYITPAVDTNL
                     DQYSALILYGPMRSITSLVERFKRELDQSKVVLWFTEQLPSPNASKPLQTLLAQTRYL
                     IQSTLETDRLLNSTSLHRIVTPFLKRSGRLRAVGEAITLYDNHLLAFLSVFTKRHACF
                     FQANHIPARVIPMGYHPLFGRDLHLEKDIDVLFIGSTVDRRRRTILHQLREWYADQGV
                     RFVIFDGSPENGFVFGKRRMEMLNRARIFINIFRQPWDDPTFRLLLAGANHTMLLSEK
                     VDSSSRGPFEANVHFGECELGCLGEQTLEYLAHPAEIERITRANAEYIRQDLTMDRQV
                     RKIIARLKRI"
     gene            complement(2257..3480)
                     /locus_tag="VN363_04650"
     CDS             complement(2257..3480)
                     /locus_tag="VN363_04650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ83831.1"
                     /translation="MIDEEMLTEFDGHEEEGANPAIARLLELGRQKTFVTIDDILTFF
                     PDAEQDVDQLEEAFAALISAGIPYLDEVAAVGPSEEDLSEEEDVEEDRRAQLAVDDNY
                     LANIDTDDTIGLYLKEVGRVPLLTAPEEVDLAQRIEAGKLAREELAKGNVTQRRRQEL
                     QLLIEDGWAAREHLITANSRLVISVAKKYMGRGVPFLDLIQEGNIGLIRAAKKFDYRR
                     GHKFSTYATWWIRQAVTRAIADQGRTIRVPVHMGDQINKLLRVQHQLTQRLGRDPSVD
                     ELAEALDVTPQKVENMIQVARRPLSLETPTDDEEDSVLGDFIQDEDAAAPDETATYNL
                     LREHLDSVLNGLPPREVRILQLRYGLLDGQAYTLEEVGRKMGVTRERVRQIEAQALSR
                     LRHPAIRRKLREYLG"
BASE COUNT          731 a         1042 c          992 g          804 t
ORIGIN      
        1 gcacaggctt cccccacccc tcccccaaca gctgcctcag cacagccggc gtctacttct
       61 gagccgcgag tttccagtat tgccaccggc aaaccggcca tcattgtcga ccacacttcg
      121 gtggccctgt ttgagcaaat ccccgaaaag tacctggccg cagcccgcga tttgcgcatg
      181 gtgtacggcg atcgctcggt gggctggaac atcaaccagt acctggactg cctggcccaa
      241 cccagctggg gggattctcc cgcattctgc cgccgggatt atcttcccgg cagtgacgag
      301 ttcacgactt atggccccaa ccgcgcggtt tcaggcgaag ctggcgaaag catccgcttc
      361 acccccgatc cggtgcgcta taaccgcgcc aactggctct tcgctgagat cagcgacagc
      421 tggtccgact ataccgaaag gttcatcacc gaatttgcgc ccggctacct ggagagcatg
      481 gatgtgctgt cttaccagtt cacctatctg catgtttctg gggataatgg cattgccgat
      541 ccggctaccg gttattttgc cgataacccg gatagttttg atatcgtcga cctggaagca
      601 ttcatcgccc agcatcccga gaagaccttt ttcttctgga ccaccagcct ggcgcgcggg
      661 ataggcactc aggaagccac cgatttcaac gaccagatgc gcgattatgt cggtaagaac
      721 ggcctgatcc tgttcgatgt ggcggatatc gagtcgcaca cacctgccgg tgagccgtgc
      781 tatgacaacc gggatggggt ggaattctgt accaaaaatg gcaactgcga gaattttccg
      841 gacgatgggg tagagttcct cgccctgtgc cgggaataca ccaccgagct ggagggcggg
      901 catctcgggg ccgtctcagg cggcggcatc cagcttgcca aagccttctg ggtgcttatg
      961 gctcagattg ccggctggca gccgtaaaca atctgtcacc gcatggttgc gattggcggg
     1021 cgggagcgct atatccgctt cagtcgggca atgatttttc tgacctggcg gtccatggtc
     1081 aggtcctggc ggatatactc tgcatttgcc cgggtaatcc tctctatttc cgctggatga
     1141 gcaaggtatt ccagggtttg ttcacccagg cagcccaatt cgcactcccc aaaatggacg
     1201 ttcgcttcaa atggtccgcg gctgctggag tctacttttt ccgagagcag catggtgtga
     1261 ttggcgccgg ccaggagcaa ccggaaagtc ggatcgtccc agggctggcg gaagatattt
     1321 ataaatattc tggcgcgatt taacatctcc atccggcgct ttccgaaaac gaatccattc
     1381 tcgggcgagc catcgaagat cacaaaccgc acaccctggt ctgcatacca ttcgcgtaat
     1441 tggtgcagga ttgtccttct gcgtctgtca accgtcgagc caataaacag gacatcgatg
     1501 tctttttcga ggtgtaaatc acgcccaaat aacggatggt aacccatggg gatcaccctt
     1561 gccgggatgt gattggcctg aaagaagcag gcgtggcgtt tcgtaaagac actcagaaac
     1621 gccagcaggt gattatcgta cagggtgatg gcttcgccca cggcacgcag ccgcccggac
     1681 cgcttcagga agggggtaac gatccggtgt aaactggtgc tgttgagcag tcgatcggtt
     1741 tccagtgtgc tttggatcag atagcgggtt tgagccaaaa gggtttggag aggtttcgac
     1801 gcatttgggg atggcagctg ttccgtgaac cataagacga ctttcgattg gtccagctcg
     1861 cgcttaaatc gttcaaccag cgaggtgatg gacctcatcg ggccatacag gataagggcc
     1921 gaatactggt ccaggtttgt atccacggct ggcgtgatgt aatcgcaagg atagccggca
     1981 tgtttgaagg catccatgat cgggacgctg aaaccccagg gatctgcttc ggtttctacc
     2041 agggcaacct gacggatttc catattaccc acctgtaatc aagtcatgcc cggcaggagg
     2101 acctcgtcct cctgccagga tatgcgtgac actttaattg tcagagttat taagttggag
     2161 gggctgtgat gagttccaga ctcgacaata aagcctggtc gtgcagtagt gttgctgtag
     2221 cctgcaggta tacgtgcagt tatgcgggca taccggttag cctaaatact cgcgcagttt
     2281 cctccggatg gcggggtggc gcagacggga tagggcctgg gcctcgatct ggcgaacgcg
     2341 ctcgcgcgtg acgcccatct tgcgccccac ttcttccagg gtgtaggcct ggccgtccag
     2401 cagcccatag cgcagttgta aaatgcgcac ttcgcgcggc ggcagcccgt tcaaaaccga
     2461 atccaggtgt tcgcgcagca ggttgtaggt tgccgtttcg tccggggcag ccgcgtcctc
     2521 atcctggatg aaatcaccca gcaccgaatc ttcctcatcg tcggtcggcg tctccaggga
     2581 gagcggccgg cgcgccacct ggatcatatt ttccactttc tgaggcgtca catctagggc
     2641 ttccgccagc tcatccaccg agggatcccg ccccaggcgc tgggtaagct ggtgctggac
     2701 gcgcagcagc ttgttgatct ggtcgcccat atgcaccggg acgcggatcg tgcggccctg
     2761 atcggcaatg gcgcgcgtca ccgcctggcg gatccaccag gtcgcgtaag tgctgaattt
     2821 atgcccgcgg cggtagtcga acttcttggc cgcccggatc aaaccgatat tgccttcctg
     2881 gatcagatcc aggaaaggca cgccgcgccc catatacttt ttcgccaccg agatcaccag
     2941 ccgcgagttg gctgtgatca ggtgttcacg agctgcccag ccgtcttcga tcaacagctg
     3001 cagctcctgc cggcggcgct gagtgacatt gcctttggcc agctcttcgc gggccagttt
     3061 gccggcctca atccgctgcg ccaggtctac ttcttcggga gcggtcagca gcggtacccg
     3121 cccgacctct ttcagataca gaccgatggt atcatcagta tcgatattcg ccagataatt
     3181 atcgtccact gccagctgag cccgccggtc ttcttcgaca tcttcttcct cgctcaggtc
     3241 ttcctccgaa ggaccgaccg cggcgacttc gtcgaggtag gggatcccgg cgctgatcaa
     3301 tgccgcaaag gcttcctcga gctggtcgac atcctgctcg gcgtccggga aaaacgtcag
     3361 aatatcatca atcgttacaa acgttttttg acgccccagc tcaagcagcc gggcgatggc
     3421 gggattcgct ccttcttctt catgtccatc aaattctgtc aacatctctt catcaatcat
     3481 gggtactcca aaacctgctc ccccgcagga aaatatcagt aagtcaatct cagagctaaa
     3541 accgctattt agaacacacg atcatagaa
//
LOCUS       DATMXK010000254         4610 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_164132, whole genome shotgun sequence.
ACCESSION   DATMXK010000254 DATMXK010000000
VERSION     DATMXK010000254.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4610)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4610)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4610
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164132"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(131..1141)
                     /gene="galE"
                     /locus_tag="VN363_04655"
     CDS             complement(131..1141)
                     /gene="galE"
                     /locus_tag="VN363_04655"
                     /EC_number="5.1.3.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004220743.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003978 - UDP-glucose 4-epimerase activity
                     [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose 4-epimerase GalE"
                     /protein_id="HWQ83832.1"
                     /translation="MNILVTGGAGYIGSHTCVELLQAGYEVIVVDNLSNSKEEALRRV
                     QEITGRTITFYRVDLLDKPGLEAVFSAHQIDSVIHFAGLKAVGESVSIPLRYYQNNIT
                     GTLNLCQIMQQYAVKKLVFSSSATVYGETNPSPLREDYPLSATNPYGRSKLMIEEILR
                     DLHRSDPEWDIALLRYFNPVGAHSSGRIGEDPNGIPNNLLPYITQVAVGRLEQLAVFG
                     NDYPTPDGTGVRDYIHVVDLAIGHLKALEKLAARPGAVAYNLGTGHGYSVLDVVNAFS
                     KACGRPIPYRVTARRPGDVAVSVADPTRATQELGWQAQLDLDDMCTDSWRWQQQNPEG
                     YL"
     gene            complement(1169..2434)
                     /locus_tag="VN363_04660"
     CDS             complement(1169..2434)
                     /locus_tag="VN363_04660"
                     /inference="COORDINATES: protein motif:HMM:NF024742.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SLC45 family MFS transporter"
                     /protein_id="HWQ83833.1"
                     /translation="MKFSYPKTFLLGFGFFGVSVIWGTYNAFVPLILANKFNLAPALI
                     GFFMTLDNIAALLIQPSVGAWSDRLRTPLGRRLPYILIGAPISAVVFGLIPLVNVLPL
                     FVACTTTLLISMAFWRTPVIALMPDITPSKYRSQANGIINFMGGVGSIISFLGGATLY
                     EMNQSYPFWLGSVLVIIASLMVLLFIREPKNYQSAEKQPGMLQSIKEVINDPDKSALR
                     MLAAIFFWFVAYNAIEAFFTLYATNHLGQTEADGARLLGQLSLLFVLFALPSGYIGGR
                     IGRRPTIMIGITFMAIAMLVMYFTPVGVLTTPLTRLPVLGNVPVIGVILMLAGIAWAL
                     ININSLPMVVDMTTAARLGTYTGLYYLASTLAAIAGPNINGWIIQLGGRNYNTIMLVA
                     PIFMLAAWVLISGVKRGEAVQVIEPIPAD"
     gene            complement(2521..3318)
                     /gene="trmD"
                     /locus_tag="VN363_04665"
     CDS             complement(2521..3318)
                     /gene="trmD"
                     /locus_tag="VN363_04665"
                     /EC_number="2.1.1.228"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561179.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0052906 - tRNA
                     (guanine(37)-N1)-methyltransferase activity [Evidence
                     IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA (guanosine(37)-N1)-methyltransferase TrmD"
                     /protein_id="HWQ83834.1"
                     /translation="MPALQFDVFTLFPDVFGPYLQSSILLRAQQRQLILIQLHNIRDW
                     TTDRHHITDDEPYGGGGGMVMKPEPVFTAIESVLGSPPICPVILLTPQGRVFNQNVAR
                     QMVQPALEHLASPGEMPVPRLALLCGRYEGIDERVRQYLVSDEISIGDFVLTGGELAA
                     LVVIDAISRLIPGVLGDPDGAEDDSHASGLLEYPHYTRPPEFRGYPVPEVLLSGNHAR
                     IARWRREQALLRTWLRRPDMLMNAPLSDSDRKFLAQLEEQQTRNTDA"
     gene            3469..>4610
                     /locus_tag="VN363_04670"
     CDS             3469..>4610
                     /locus_tag="VN363_04670"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ83835.1"
                     /translation="MTRRTRRPSFFRIALLVAVIGLLLYINQVVVPATPPLFIPTPTP
                     TLSPEAYLTEADELYSSGKINAAINAYKSAIDVDPSNVATFVTLARLQVFAGQYDAAI
                     ENTQNALLKNPNNSLAHAVQGWALGFQGKFGEAELAIRKALELDGNNALAHAYYAEIL
                     INQNDFELVDKAGEESRLARDMDSSLLEVHRARGIVLLNTNNVDEAIEEFKAALAINK
                     NIADIHLYLGVAYKAQGDYSLAEESLLAAYALNPQDTVALTEIARAYFADGRYRQAAQ
                     YAEEAVRVAPADPTLHGNLGIMHYKNEEYSRAIPEFELAVRGGTTEDGTAVEGLPLAY
                     GRVAEYYWYYGFALARSNQCAQAVPIFQSLLTNLQNDEIAVFNATAG"
BASE COUNT         1107 a         1201 c         1274 g         1028 t
ORIGIN      
        1 atatagggct gcccgtaatc tgggcctgta ggacccggtc attagattgt gttcagatcg
       61 aagattgaaa tgacgatggg cgtctgcagt gcgggcagat aagttcatcc actggtctgg
      121 cacaatattt ttacagataa ccttctggat tttgctgctg ccagcgccag gaatcagtgc
      181 acatatcatc gagatccaat tgcgcctgcc agcccagctc ttgagtggca cgtgtagggt
      241 cggcgacgct gacagcaaca tcgccaggcc ggcgcgccgt gacgcggtag ggaataggtc
      301 tgccgcaggc ttttgaaaat gcgtttacca catcgagcac actgtagccg tgtccggttc
      361 cgagattgta ggcaacagca cccggtcgcg cggccagctt ttccagggct ttgagatgcc
      421 cgatcgccag atcaacaaca tggatataat cgcgcacccc agtgccgtca ggagtgggat
      481 aatcgttccc aaatactgcc agctgctcaa gccgcccgac ggctacctgg gtgatatagg
      541 gcagtaaatt attggggatg ccattcgggt cttcaccgat ccgcccgctg gaatgagcac
      601 cgacaggatt gaaataacgc agcagggcaa tatcccattc gggatcggag cggtggagat
      661 cgcgcaggat ctcctcgatc atcagcttgg aacgaccata ggggttcgtc gctgagaggg
      721 gatagtcttc ccgcagaggg gatgggtttg tctctccgta cactgtcgcc gaagagctga
      781 ataccagctt tttgaccgcg tattgctgca tgatctggca caggttgagt gtccccgtga
      841 tattgttctg ataataacgc agcgggatcg ataccgactc gcccactgct ttcaatccgg
      901 caaagtggat gacactatcg atctggtgag ccgagaaaac agcttccagc cccggtttat
      961 ctaaaaggtc cacccggtaa aaagtgatgg ttctaccggt aatctcctgt actcgccgca
     1021 gtgcttcttc tttgctgtta gacaggttat cgacaacgat gacctcataa ccagcctgaa
     1081 gcagctccac gcaggtatgg ctgccaatat aacctgcccc gccagtaacg agtatattca
     1141 taagtgtttc cttctctctt cggtttaatc aatctgctgg gataggctcg atgacctgta
     1201 ctgcttcgcc gcgcttcaca cctgagatca gaacccaggc tgccagcatg aatattggcg
     1261 ccaccagcat aatggtattg taattacgcc cgccgagctg gatgatccaa ccgttgatat
     1321 taggaccggc aattgccgcc agggtagaag ccaggtaata caggcctgtg taagtaccca
     1381 ggcgagcagc ggtggtcata tccactacca tcgggagtga gttgatatta ataagcgccc
     1441 aggcaatgcc tgccagcatg agaattaccc cgatcacggg tacattacct aacaccggca
     1501 ggcgtgtgag tggggtcgtc aaaacaccga ccggtgtgaa atacatgacc agcatggcaa
     1561 tcgccatgaa tgtgatcccg atcattatcg tcgggcggcg accaatccgc ccaccaatat
     1621 acccggaggg cagtgcgaac agcacaaaaa ggagtgaaag ctggcctaat aagcgcgccc
     1681 catctgcctc ggtctgtcct aaatggttcg tggcatataa agtgaagaaa gcctcaatgg
     1741 cattataggc cacaaaccag aaaaatattg ctgcgagcat gcgcaaggcg cttttgtccg
     1801 gatcgttaat gacctctttg atactctgca gcatacccgg ttgtttctcc gcagattgat
     1861 aatttttcgg ctcacggata aataatagca ccatcaggct ggcgataatc accagaactg
     1921 agcccagcca gaagggataa gactggttca tctcataaag cgtagcaccc cccaggaagg
     1981 agatgatcga cccgactcca cccataaagt taatgatgcc attggcctgg gatcgatatt
     2041 ttgaaggagt gatatccggc atcagggcga tcacgggcgt gcgccagaag gccatactga
     2101 tgagcagtgt cgtggtgcag gcgacgaaca gcgggagcac gttaacgagc ggtatcaggc
     2161 cgaataccac ggcactgata ggcgctccga tcaagatgta gggcaggcgc cggcccagcg
     2221 gggtgcgcaa gcgatcggac caggcgccaa cagatggctg gatcaataac gcggctatat
     2281 tatccagggt catgaagaag ccgatcaagg caggggctaa attgaattta ttggccagaa
     2341 ttaatggcac aaacgcattg taggtccccc agataacact cacaccaaaa aagccaaatc
     2401 ccagaagaaa tgtttttggg tagctgaatt tcaccttgtg cctccatgtt cttaatcgtt
     2461 gttcagaaat ggttcagcct gcccattaaa acaggccatg tctgacttta ttagctaagt
     2521 ttacgcatct gtattccggg tctgttgttc ttcgagctga gccaaaaact tgcggtcact
     2581 gtcactcaag ggggcattca tcagcatgtc cggtcgccgg agccaggtac gcagtagcgc
     2641 ttgctccctt cgccagcgag ctatccgggc gtgattgccc gagagcagca cttctgggac
     2701 ggggtagccc cgaaattctg gagggcgggt gtagtggggg tattcgagca gcccagaagc
     2761 atgtgaatcg tcttcggccc catctggatc gcccagcaca cccgggatca ggcggctgat
     2821 ggcatcgatc acgaccaggg cggccagctc cccgccggtg agcacaaaat ctccgatcga
     2881 aatctcatcg ctgacaagat actgccggac gcgctcgtcg atgccttcgt accgcccgca
     2941 caacagcgcc aggcgcggca cgggcatctc tccgggtgat gccaggtgct ccaatgcagg
     3001 ctgcaccatc tggcgagcga cgttttgatt gaatacccgc ccctggggag tgagcaggat
     3061 caccgggcag atgggtggtg atcccagcac actttcgatc gcggtgaaga ccggctctgg
     3121 tttcatcacc attccaccac cgccgccgta aggctcatca tcggtgatgt gatggcgatc
     3181 ggtcgtccag tcgcggatat tgtggagctg gatgaggatc agctggcgtt gttgggcgcg
     3241 cagcaagatg ctgctctgta aataaggtcc aaaaacgtcg ggaaacaggg tgaaaacatc
     3301 aaattggagg gctggcacag tcacaaaccg attttagccg caagcgtttc gcttggcaag
     3361 gaagaatggg tgggcgttct tcattaagag ccattcatcc ctatcttgac tcggtttctt
     3421 gaaagatggt aagatgccat tatgtatcta cgagggagca agtggtcaat gacgcggcgc
     3481 acacgccgcc caagtttctt tcgcatagct ttattagtgg cagtgattgg attactgttg
     3541 tatatcaacc aggtggtcgt tcccgccacg ccgcccttat tcatcccgac gccaactccc
     3601 accctcagcc ctgaggccta tctgacggaa gccgatgagc tttacagctc gggaaaaatt
     3661 aatgccgcga tcaatgccta taaatccgcg attgatgtgg accccagcaa tgtggcgact
     3721 ttcgtcaccc tggcgcgcct gcaagtattc gccgggcagt atgacgcggc gatcgaaaac
     3781 acacagaacg ccctgttgaa gaatccgaat aattctttgg ctcatgcggt acagggctgg
     3841 gcgttgggat ttcagggcaa attcggggag gctgagcttg ctattcgtaa ggctttggag
     3901 ctggatggca ataacgctct ggcgcacgcc tattatgctg agatcttgat caaccagaac
     3961 gactttgagc tggtggataa agccggggag gagtcgcgcc tcgctcgcga tatggacagc
     4021 tcccttctgg aagtgcaccg ggcccgtggg attgtgctgt tgaataccaa taatgtcgat
     4081 gaagcgattg aggaattcaa ggcagccctg gcaattaata aaaatatcgc tgacattcat
     4141 ctatatctgg gtgtggccta caaagcccag ggggattact ctctggccga agaatcactg
     4201 ctggcggcgt atgccttgaa tcctcaagat acggtcgcat taaccgagat cgcccgcgcc
     4261 tatttcgcgg atgggcgtta tcgccaggca gcccaatatg ccgaagaagc cgtgcgggtt
     4321 gcgccggctg atcctaccct ccacggcaat ctggggataa tgcattacaa aaatgaagaa
     4381 tatagccggg cgattccaga gtttgagctg gccgtacgcg gcggtacaac tgaggatggg
     4441 actgctgtag aaggcttgcc gctcgcttac gggcgagtgg cagaatatta ctggtattat
     4501 ggttttgctt tagcacggtc aaaccaatgc gcgcaggctg tgccgatctt ccagtcgtta
     4561 ttgaccaatt tacaaaatga cgagatcgct gtttttaatg ccactgccgg
//
LOCUS       DATMXK010000255         3141 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_128520, whole genome shotgun sequence.
ACCESSION   DATMXK010000255 DATMXK010000000
VERSION     DATMXK010000255.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3141)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3141)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3141
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128520"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1941
                     /locus_tag="VN363_04675"
     CDS             <1..1941
                     /locus_tag="VN363_04675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83836.1"
                     /translation="DLVRDHLKNYTGEILPTFFENGGKYRLGVSVYLQVIPYIMFGKS
                     VFVTRAVSVLVSLLAAAAVGLALRQLFKVRIWWAGVLFLSLTPAWFLHSRTAFECAAA
                     AAFYAASLYCYLRYRLQSPPSLYAALAFGALAFYTYSPFQIIAPASFLILFVLDWRYH
                     LENRRMAVPAIGLLVLLGLPYLRFVLTHSQENLSSLAALNSPWARPLPLGEKMRLTAV
                     EYLRALNPTYWFTPDEGGLARHQMNDKAYLTRWTFPFLLLGFGQAVWRARPGRKQDGQ
                     PAVNQELAPAYRVLLVSVFAIPLGAALVQVGITRLLGLVIPAALLTTLGIQRCIVWLG
                     KRRVPVYLLESGLLILLAIANFALLREALWKGPTWTTNYGMGGMQYGASQVFDAAQEY
                     ITEYPDTRMIVSPTWANGTDVLKRFFLPDDLPVELGSISGHLQNQKALDRQMLFVMTP
                     DEYEAVLQSGKFVNIQVEQTLPFPNGQTGFYFVRLDYAPNIQELMAGELAERRKLQAA
                     EAQVDGELVQFSHSTLDIGTIEQAFDGDTNTLLRTWEANPMVIEMRFPRPRRLSGIEL
                     ILGSAQIEVTLRLSDPSQGQPVVLSQVVRGTVQQPRVELTFPEAYLVQTLRLELRDVN
                     QGEPGHVHLWEIDLQTQAGGES"
     gene            1938..2972
                     /locus_tag="VN363_04680"
     CDS             1938..2972
                     /locus_tag="VN363_04680"
                     /inference="COORDINATES: protein motif:HMM:NF024989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-binding dehydrogenase"
                     /protein_id="HWQ83837.1"
                     /translation="MRAVMFHEHGGPETLIYSEIETPSPGPGEVLVRLHAAALNRLDL
                     WVRQGWAGLKLQYPHIPGADGAGEVAAIGPGVEGWSTDEWVVINSNLSCGTCEACLAG
                     LDNRCTKWELLGETRRGTYAEYVVVPAKNLLRVPQDFSLQAAAAASLAYHTAWHSLII
                     RGALQPGETVLIVGAAGGVNTACLQIARLAGAVTYVVGSDARKLEFAASLGADYLIDR
                     SKEANWARTIFQLTNRRGVDVVVDNVGTTFPLSFRAARKGGRILTVGNTGGAVFEIDN
                     RYIFSKHLSLLGSTMGTQHDFLRVMELIYNGRLRVPVDRVYPLSEARQAQEYLAAGEQ
                     LGKVVLDILG"
BASE COUNT          580 a          855 c          998 g          708 t
ORIGIN      
        1 gacctggtcc gcgaccacct gaaaaactac accggggaaa ttctgcccac tttttttgaa
       61 aatggcggga aataccgcct gggtgtttcg gtttatttgc aggtaatccc ttatattatg
      121 ttcggaaaat ccgtctttgt gacgcgggcg gtgtcggtgc tggtttcact cctggcagcc
      181 gcggcggtcg ggctggctct gcgccagctc ttcaaggtgc ggatctggtg ggcaggagtc
      241 ctgtttctct cattaacccc ggcctggttt ctgcactcac gaactgcatt cgagtgcgcg
      301 gctgcggctg cgttctatgc cgccagcctg tattgttacc tgcgttatcg cctgcaatcg
      361 ccgcccagcc tgtatgctgc tctggctttt ggggcgctgg ctttctatac ctacagcccc
      421 tttcagatca ttgctccggc aagcttcctg atcctgttcg tgctcgattg gcgctatcac
      481 cttgaaaacc gccggatggc agtccctgcg attgggttgc tggtcctgtt ggggctgccg
      541 tacctgcgct ttgtcctgac ccacagccag gagaatctca gcagcctggc tgcgttgaac
      601 tcgccctggg ccaggccgct gccgctgggc gaaaaaatgc gcctgactgc ggtggaatac
      661 ctgcgcgctc tgaatccaac ttactggttc acgccggatg aaggcggcct ggcgcgccac
      721 cagatgaatg ataaggcgta tctcacccgc tggactttcc ccttcctttt gttaggattc
      781 gggcaggcgg tctggcgggc gcggccgggt cgcaaacagg atgggcaacc tgctgtaaat
      841 caggaattag ccccggctta ccgggtgctg ctggtctccg ttttcgcaat cccgttgggc
      901 gcggcgctgg tgcaggtggg gatcacgcgc ttattaggtc tggtgattcc ggccgcgctg
      961 ctcaccaccc tgggtataca gcgctgcatc gtctggctgg gaaagcggcg agtgcctgtg
     1021 tacctgttgg aatctggcct attgatatta ttagcaattg ccaatttcgc actgctgcgg
     1081 gaagctttgt ggaagggccc tacctggacc acgaattacg gcatgggcgg catgcagtac
     1141 ggcgccagcc aggtctttga cgcggcgcag gaatatatca ccgaataccc ggatacccgg
     1201 atgatcgtct caccgacctg ggcaaatggc acagacgtgc ttaagcgctt tttcttaccg
     1261 gatgacttac cggtagaatt aggcagtatc agcggccacc tgcagaatca aaaagcgctc
     1321 gaccggcaga tgttgtttgt gatgacgccg gatgaatatg aggctgtcct gcagagtggg
     1381 aagtttgtca atattcaggt cgagcaaaca ttaccctttc caaatggtca gactggtttt
     1441 tattttgtgc gtctggacta cgccccaaat atccaggagt taatggcggg ggagctggca
     1501 gagcggcgca aactgcaggc ggctgaagcg caggtcgatg gcgagctggt gcagttcagc
     1561 cattcaaccc tggatattgg caccatcgag caggcattcg atggggatac caataccctg
     1621 ctgcgcacct gggaagccaa tccgatggtg atcgaaatgc gctttccccg gccgcggcgc
     1681 ctcagtggaa tcgagctgat ccttggcagt gctcagatcg aggttacatt acggctcagt
     1741 gatccctcgc agggccagcc agtcgtcctc agccaggttg tgcgtggcac agtccagcag
     1801 ccgcgggtgg agttgacctt cccggaggct tatctggtgc agaccctgcg ccttgagctg
     1861 cgggatgtta atcagggcga gccgggtcat gtacacctgt gggagatcga cctgcaaacg
     1921 caggcaggag gggaatcatg agagcagtca tgttccacga gcatggcggg ccggaaaccc
     1981 ttatttattc tgagatcgag acgccctcgc cgggaccagg agaggtgttg gtccgcctgc
     2041 atgcggcggc tttgaaccgg ctcgatctgt gggtgcgcca gggctgggca gggctcaaac
     2101 tgcagtaccc gcatatcccc ggcgcggatg gcgcggggga ggttgccgct attggcccag
     2161 gtgtggaggg ctggagcaca gacgagtggg tggtgatcaa ttccaacctg agctgcggca
     2221 cctgtgaggc ctgtctggct gggctggata accgctgcac taaatgggag ctgctgggcg
     2281 aaacccgccg cgggacgtat gcagagtatg ttgtcgtccc ggcaaagaat ttgctgcggg
     2341 tcccccagga ttttagcctg caagctgccg cggcagccag tctggcttat cacactgcct
     2401 ggcactcgct gatcatccgg ggggcgctgc agcccggaga gacagtgctg atcgtgggcg
     2461 cggccggcgg agtaaataca gcctgcctgc agattgccag gctggccggg gcggtgacct
     2521 acgtggtcgg ctcggatgcc cgcaagctgg agtttgccgc ctcgctggga gcagattacc
     2581 tcattgatcg ttccaaagaa gcaaattggg cccggaccat attccagctg accaaccggc
     2641 gtggggtaga tgtggtagta gataatgtgg ggacgacttt tccgcttagc tttcgagccg
     2701 cgcgcaaggg tgggcggatc ctgaccgtag gcaatacggg tggagcggtg ttcgagatcg
     2761 acaaccggta tattttcagc aaacatctca gcctgctcgg ctcgacgatg ggtacgcagc
     2821 acgatttcct gcgtgtgatg gagctgatct ataatggccg gttacgcgtg ccggtggacc
     2881 gggtttatcc gctgtccgag gcgcgccagg ctcaggaata cctggccgcg ggcgagcagc
     2941 tgggtaaagt tgtgctggat atcctgggct gagcaatggt acccgaatcg acttcaacga
     3001 aggaagccgc gattaaccgg ccggcgggcg taaccttgct gggctggggg gtgttaatcc
     3061 ttgtatgcct gaacctggta cgtttttttc aggcagtcct gcagtgggat ttcttgaccg
     3121 ggctgccagg cgtttccccg c
//
LOCUS       DATMXK010000256         4063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_68433, whole genome shotgun sequence.
ACCESSION   DATMXK010000256 DATMXK010000000
VERSION     DATMXK010000256.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68433"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..285)
                     /gene="tsf"
                     /locus_tag="VN363_04685"
     CDS             complement(<1..285)
                     /gene="tsf"
                     /locus_tag="VN363_04685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="translation elongation factor Ts"
                     /protein_id="HWQ83838.1"
                     /translation="MAITTEQIKELREATGAGILDCRKALEQSNGDFDKAVDYLRQKG
                     LATAAKRADREASEGTVELYSHGNGRVGVMVEVNCETDFVARSEPFRKFAH"
     gene            complement(288..1283)
                     /gene="rpsB"
                     /locus_tag="VN363_04690"
     CDS             complement(288..1283)
                     /gene="rpsB"
                     /locus_tag="VN363_04690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01011.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S2"
                     /protein_id="HWQ83839.1"
                     /translation="MALIPMKALLESGVHFGHRTHKWHPKMKPYIFTERNGIHIIDLQ
                     KTVKGLEQAYALVRDTVSEGGSILFVGTKRQAQETIQAEAIRCGMPYVTQRWLGGMLT
                     NWRTIRQRINELERLEKMRDHGDFERITKKEALILSREIERLDTRLGGIRALARLPEM
                     LFIVDVHREVTAIHEANLLEIPIVAMVDTNCDPGPIDYVIPSNDDAIRAIKLIVGKMA
                     DAVLEGKAVRKDAVEEERPVEVRIPGQAAIEEEDLSDEDLLGAATLAKISTRPAVHTE
                     LEVEEEVALVAETEDENDYAPDALGGDEVVAAEAAVADDVADADEDDTAVVEDEE"
     gene            1489..2331
                     /locus_tag="VN363_04695"
     CDS             1489..2331
                     /locus_tag="VN363_04695"
                     /EC_number="6.3.4.15"
                     /inference="COORDINATES: protein motif:HMM:TIGR00121.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004077 - biotin-[acetyl-CoA-carboxylase]
                     ligase activity [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="biotin--[acetyl-CoA-carboxylase] ligase"
                     /protein_id="HWQ83840.1"
                     /translation="MDQAALTEGLAEIPLGWLEYHPRTSSTNDEASRLVQAGCPDLSL
                     VVAGEQTSGRGRLNRRWFTPPGAALAFSLVLVPDNAWLALDAQEVGTVLPRVTALGAV
                     AVCQALETRYSLAAQIKWPNDVLVNQRKLAGVLVETTWEGELLKAVILGIGLNIASHS
                     VPPAAELLFPATSLEDCLMAKMDDAERLALLKDILERIREWRVKVSGVEFIQAWQTKL
                     AYLDEWVWIVPAQAQAGDSQSAVKVRILGLEADGSLRVEDQNGTVRRYISAEVHLRPH
                     STDF"
     gene            2373..2579
                     /locus_tag="VN363_04700"
     CDS             2373..2579
                     /locus_tag="VN363_04700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83841.1"
                     /translation="MLDDFRSQADTPFFDEDNQPEEITYETETRQEFLGMTPFQRFVI
                     AIMILLITLLLSSFCLLVTGKVAF"
     gene            complement(2645..>4063)
                     /locus_tag="VN363_04705"
     CDS             complement(2645..>4063)
                     /locus_tag="VN363_04705"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003916 - DNA topoisomerase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase C-terminal beta-propeller
                     domain-containing protein"
                     /protein_id="HWQ83842.1"
                     /translation="ALQIFIEHRRLVIVRRSEFELTRARERAHILAGLLIALANLDDV
                     ITTIRQSPDADIAKERLVSRFNLSERQAQAILDMQLRRLAALERQKIEDEQRLTLERI
                     AYLEDILQSPAKVLELIKSDLDEVIEKYNDERRTQIAHGASDVFNEEELVRDEAVLVS
                     LTQRGYIKRVAAKAYRAQGRGGRGVTGHHMKDEDEILILLPANTLNTVLFFSNKGKVY
                     SVKAYQIPEADRTGRGVPVVNILSLGAAETITAALAVPQFDQANFCVMATHRGRIKRL
                     PLSELESVRPSGLIAMNLDANDELGWARQTNGKDDIILVTELGQALRYSEQEIRPTGR
                     SSAGVTAIRLDKADAVTSMEVVEKDGYLLIVTTRGFGKRTSLTEYPVKGRATGGVQTI
                     DKKALATIGRITSARVVQAADDLTMISANGVVLRTKVQAVSQSGRAARGVRVMEVGEG
                     DSVASLARISDADLRQVGAGEE"
BASE COUNT          839 a         1092 c         1182 g          950 t
ORIGIN      
        1 gtgggcgaat ttccggaaag gttcagagcg ggcaacaaaa tcggtctcac aattgacttc
       61 gaccatcacg ccgacccgtc cgttaccatg ggaatagagc tccacggtgc cttcggaggc
      121 ttcgcgatcg gcgcgcttgg cagccgttgc cagacccttt tggcgcagat agtctactgc
      181 tttgtcaaaa tcaccattgg attgttccaa tgccttgcgg cagtccagaa taccggcacc
      241 ggttgcttca cgcagttctt tgatctgttc agtagtgatc gccatgctta ttcttcgtcc
      301 tcaacgacag cggtatcgtc ttcgtcagcg tcagcgacgt cgtcagcgac tgcggcttcg
      361 gctgcgacta cttcgtcacc gcctaatgca tcgggagcat aatcattttc gtcctcggtt
      421 tctgccacga gtgctacttc ctcttcaact tcaagctctg tgtgcacggc cgggcgagta
      481 gagatttttg ccagggtggc ggcgccaagc agatcctcat cggacagatc ttcttcttcg
      541 atggctgcct ggccaggtat tcgtacctcg accgggcgtt cctcttcaac ggcatctttg
      601 cggacagctt tgccttccaa aacggcatcg gccattttgc caacgataag cttgatggcg
      661 cggatagcgt catcattgga ggggatgaca tagtcgatcg gaccgggatc acagttggta
      721 tccaccatcg caacgatcgg gatctcaagc aagttcgctt cgtggatcgc ggttacttca
      781 cggtgaacat cgacaatgaa gagcatttcg ggtaagcggg cgagggcgcg aataccaccc
      841 agacgagtat ccaggcgctc aatctcacgg ctgaggatca aggcttcctt tttagtgatc
      901 cgctcgaaat caccgtggtc gcgcattttt tccagccgct ccagctcatt aatccgctgg
      961 cgaatggtgc gccaattggt aagcatgccg cccagccagc gctgggtgac atagggcatc
     1021 ccacagcgaa tggcttcagc ctggatcgtt tcctgagcct ggcgttttgt gccgacaaac
     1081 aggatggagc cgccttcgga aacggtgtcg cgcaccaagg cgtaagcctg ctcgagacct
     1141 ttgacagtct tttgcaaatc aataatatgg atgccattgc gttcggtgaa gatgtaaggc
     1201 ttcatcttcg ggtgccattt gtgggtgcga tgcccaaaat gcacgccgct ttcaagaagc
     1261 gccttcatgg ggataagggc catacgatat tctcctttgc gttatacttc cgcccccttc
     1321 atccccaccg gaggccgtgg ctacggcacg caaggcaggt cgagaggcgt gtaagatgtt
     1381 ggcctcaggc gaggcacaat gtctcccgcc tggggcggga gcggacggta gtataacata
     1441 gggcgggtcg attcgtctag aggatatttt gtggggtaaa atccagatgt ggatcaagca
     1501 gcactcaccg aggggttagc agaaatacct ttaggctggc tggagtatca ccctcgcacc
     1561 agttcaacca atgatgaggc aagccggctg gtgcaagctg gctgcccgga tttgagcctg
     1621 gtggttgctg gcgaacagac ctccgggcgc ggccggctta accggcgctg gttcactcca
     1681 ccaggagctg ccctggcctt cagcctggtt ttggtgccag ataatgcctg gctggcattg
     1741 gatgcgcagg aagtggggac agtcctgccg cgggtgacag cgttgggtgc cgtggcagtc
     1801 tgccaggcat tggagactcg ctatagtctc gcagcgcaaa taaaatggcc caacgatgtt
     1861 ctggtcaacc agcgtaagct ggcaggggta ctggtagaaa ccacctggga aggcgagctg
     1921 ctgaaggctg tgatcctggg gataggtttg aatattgcct cccattcggt gccgcctgcc
     1981 gctgagctgc tcttcccggc gaccagcctg gaggactgtt tgatggctaa gatggatgat
     2041 gctgaacgcc ttgcattatt gaaagacatc ctggagcgaa tccgggagtg gcgagtgaag
     2101 gtttctggcg tagaatttat ccaagcctgg cagacgaagc tggcttatct cgacgaatgg
     2161 gtatggatcg ttccagcgca ggctcaggct ggtgactctc agagcgcagt gaaagtgcgc
     2221 attctcggcc tggaagctga tggaagtttg cgagtcgaag atcagaatgg tactgtgcgg
     2281 agatacattt cggctgaagt gcatcttcgc ccgcactcaa cagatttttg accgcaggaa
     2341 gattgctcga aagcgaaata tggaggttcc aaatgctaga tgattttcgc agccaggccg
     2401 ataccccctt ctttgacgaa gataatcagc cggaagagat aacttatgaa accgagacgc
     2461 ggcaagaatt cctggggatg acccctttcc agcgattcgt gatcgcgatc atgattttgt
     2521 tgatcacact gctcctaagc tctttttgct tgttggtaac cggtaaggtt gctttttaaa
     2581 tctagggctg taatgattaa acgaacaggg cagccctgaa ggctaccctg gttggttcgc
     2641 tggtttattc ttcgcccgcc ccgacctggc gcaagtcggc atcggagatc cgggccagcg
     2701 aagccacact atctccctcg cctacctcca tcacccggac cccgcgggcg gcgcggcctg
     2761 attggctgac cgcctggact ttggtcctta aaaccacccc attggctgag atcatggtca
     2821 ggtcatcggc agcctggacc acgcgggcgg aggtgatccg ccctatagta gccagtgcct
     2881 ttttgtcgat tgtctgcacc cctccggtgg cacgaccttt gacaggatat tcggtcaggg
     2941 aggtgcgttt gccaaatcca cgggtggtta cgattaacag ataaccatct ttttcgacaa
     3001 cttccatgct ggtgacggca tccgccttat cgaggcgaat tgcggtgacc ccggcagaag
     3061 agcgcccggt ggggcggatc tcctgttcag agtagcgcag ggcctggccc agctcggtga
     3121 ccaggataat gtcgtctttg ccgttggtct ggcgcgccca gcccagctca tcattagcat
     3181 ccagattcat ggcaatcaat ccagaggggc gcacggattc taattcggaa aggggcaggc
     3241 gtttgattcg cccacggtgg gttgccatga cgcaaaaatt agcctggtca aattggggca
     3301 ctgccagggc agcggtgatt gtttcggcag ccccgaggga gaggatgttg accaccggaa
     3361 cgccgcgccc ggtccggtca gcttcgggga tctggtaagc tttgactgag tatactttgc
     3421 ctttgttaga gaagaacagg accgtattga gcgtgttcgc agggagcagg attaatatct
     3481 cgtcttcgtc tttcatatgg tggccagtca ccccgcgccc gccgcgcccc tgggcgcgat
     3541 aggctttggc tgccacgcgt ttgatgtagc ctcgctgggt cagactgacc aggaccgcct
     3601 catcgcgaac cagctcctct tcattaaaaa catcgctggc accgtgggcg atctgtgtgc
     3661 ggcgctcgtc gttgtatttt tcgatgactt catcgagatc agatttgatc agctccagta
     3721 cctttgcggg gctttgcaaa atatcttcca gataagcaat ccgctcgagg gtcaggcgct
     3781 gctcatcctc aatcttctgg cgttcgaggg ctgccagacg gcgcagctgc atatccagga
     3841 tggcttgcgc ctgacgctca gacagattga agcgggatac caggcgttct ttagcgatat
     3901 cggcatccgg cgattggcga atcgtcgtga tgacatcgtc caggtttgcc agggcgatca
     3961 ataagccagc cagtatgtgg gcgcgctcgc gagcgcgggt cagctcgaat tcagagcggc
     4021 gcacaatgac caaccggcga tgttcgataa aaatctgcaa tgc
//
LOCUS       DATMXK010000257         5199 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_63195, whole genome shotgun sequence.
ACCESSION   DATMXK010000257 DATMXK010000000
VERSION     DATMXK010000257.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5199)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5199)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5199
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63195"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..493
                     /locus_tag="VN363_04710"
     CDS             <1..493
                     /locus_tag="VN363_04710"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ83843.1"
                     /translation="IMAALIVAAVLTPRLGRTILPAIIALISFGWMSYARLIRGDILT
                     VRERDYILAAKVIGVKDHNILLRHILPNAIFPTLVYASAEIGGIVISFAALSFLGIGT
                     PVGYADWGQLLSFARDWITNLANYWYIVVYPGFALVLFVLGWNLLGDAFRDILDPKMR
                     GKS"
     gene            552..1301
                     /locus_tag="VN363_04715"
     CDS             552..1301
                     /locus_tag="VN363_04715"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWQ83844.1"
                     /translation="MGKIISAVLGIGFLIGLSGLFSTGLAAAPESGVAVQSTATPGLI
                     DQPQMVPTPDRLARPTLPANPSQADLGSQVYYMVCMACHGDQGQGLSPEWVEAWGLGE
                     ETCWQSKCHASNYPPEGFELPQYIPAVVSPGVVNRFANAQELYTYLQTRMPWQAPGTL
                     SSEEYWQLTAYLIRANGIDPGSAPLDEQSAVKILMRPVPGDQELELSGVTAVPWFWIV
                     AGAIIASAGIILLVKYVQRYFKPRRPEDETD"
     gene            1328..1936
                     /locus_tag="VN363_04720"
     CDS             1328..1936
                     /locus_tag="VN363_04720"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWQ83845.1"
                     /translation="MLAASEQGRMSDLSDRELILQLQQGSLEALGELYDHFRQMVYRT
                     ALAITSDPEAANDLLQDVFLRLYRFADRIDPQRPIEPWLYRMTANLAYTWVKRRNRWV
                     RPLEDLADWLAGSSRNSPQETAEMHDDWQRVQQAIAGLPLPQRMVVVLYYLNDLSLQE
                     ISEILDVPVGTVKSRLHYGRNSLKRSLGLQGETLPDWNYEGS"
     gene            1969..2226
                     /locus_tag="VN363_04725"
     CDS             1969..2226
                     /locus_tag="VN363_04725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83846.1"
                     /translation="MDNYLGRVLKNWAASSQPHANGRERLLANAASGRKPEQRKSGLE
                     TMRSLLSTPNYRGFHEEIRTRPTSQALIWSFQMVASTRLLA"
     gene            2239..4200
                     /locus_tag="VN363_04730"
     CDS             2239..4200
                     /locus_tag="VN363_04730"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptide ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ83847.1"
                     /translation="MFQRLTTKYILLLAALLVIGALSSCTVQPQPTASPVFPLPESPT
                     PELRALPTPSPTPEKRVLTICTLQEPVSLFIYNDQSQAARNIREAIYDGPVDMLGYRP
                     APVILTELPTQANGGVIIESLTVSAGANIVNAQGELVNLKDGVQYYPAGCRSTECALT
                     YQGQEPAQMDQMVVRYHLRPGLQWSDGSPLTADDSQYSFEVARELYPRVRQNLITHTA
                     VYQAEDSETVLWRGLPGYRDPAYGSIFFQPLPRHAWGSLPAAELLANEGANRAPLGWG
                     AYTVVEWTEGDHITLAPNPLYFRAAEGLPYFDNLVFRFISDQDQILAGLLAGECDLID
                     DTAGLETRASELNQLQEEGKLLAAYEVGTAWEHLDFGIEAYDPAALANPTLFQSPQLR
                     QAIATCIDRERLANELFFGISDVPVTYVPPTHPLANPDAARYAYDPQAGGALLEAAGW
                     LDPDQNPATPRQAQGVSRIVDGTPLAMELLASNEPEKQAAAAILQSSLAECGIQLDIR
                     LVPLEELYSPGPEGPVFGRAFQLAQFNWSNSYEPPCFLYLSSEIPGPYPEYARGWGGA
                     NASGYRNPEYDTACRLAMNSLPEDPEYAANHLRAQEIFATDLPAIPLYMRLKLVAGRP
                     DLCEMQVVPGGSSALWNLESLNYGETCGP"
     gene            4270..>5199
                     /locus_tag="VN363_04735"
     CDS             4270..>5199
                     /locus_tag="VN363_04735"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ83848.1"
                     /translation="MARYHILIVDDQRDVRVLLRSGLETLQLDLKVIDVPSAEEALLV
                     LGKEPIDLMVADVRLPGMSGLELRAQAGRRMPTTQLILITGMTDENVKQQLSQAGVAA
                     YFFKPIQIGLFLEEVCRCLGVKPGSSDNQETARPEVTPASVRQTSSNPLIELCVSQSV
                     SGCWLLTGRGKILYEYGRIAAQNEVKEFKKTLLGLLAHDRQQVHAQSGITFPCHHLFP
                     GQGRDLWVTSLGFDLVLAFALPAGEVVHQAAGMLREIGKLHASLAVREPEKGKHPAAV
                     KSAGEVSGDKELSAILGQGSKPGMRAEELDAYWD"
BASE COUNT         1097 a         1388 c         1511 g         1203 t
ORIGIN      
        1 tatcatggcc gccctgattg tggcagctgt gttaaccccc aggttgggac ggacgatttt
       61 gcccgctatt atcgccttga tctcgtttgg ctggatgagc tatgcccgcc tgatccgcgg
      121 cgatatcctc accgtgcgcg aacgcgatta tatcctggct gccaaagtga ttggcgtcaa
      181 agatcacaat attttgctca ggcatatttt acccaacgcc attttcccca ccctggttta
      241 tgcttcggca gagattggcg ggatcgtaat cagctttgcg gcattgagct tcctgggaat
      301 tggtaccccg gtcggttatg ccgactgggg acagctgttg agttttgccc gcgactggat
      361 caccaatctg gcgaactact ggtatatcgt ggtttacccc gggtttgccc tggttttgtt
      421 tgtcctgggg tggaacctgc ttggggatgc tttccgggat atcctggatc ccaagatgcg
      481 cggtaaaagt tagcggatcc tgctgaaaac caggctggaa attcactggt gataccggtt
      541 attgatggct catgggaaaa ataatatccg cagttctggg gattggattt ctgatcggat
      601 taagcggatt gttctcaaca gggctggcag cagcccctga aagcggtgtg gcggtgcaaa
      661 gtaccgccac acctggctta atcgaccaac cgcaaatggt gccgacgccc gaccgtctgg
      721 cacgtcccac cctgccagcc aatccatccc aggcagacct ggggagtcaa gtgtattaca
      781 tggtttgtat ggcctgtcat ggtgatcagg gacagggttt atcgccagaa tgggtggagg
      841 cctgggggtt aggggaggaa acctgctggc agtcgaaatg ccacgcctcg aactatcctc
      901 cggagggctt tgagctgccg cagtatattc cggctgtggt aagcccgggt gtggtgaacc
      961 gctttgcaaa cgcccaggag ctgtacactt accttcagac gaggatgccc tggcaggcgc
     1021 cgggaacgct gagctctgaa gaatactggc agctgacggc ttatctgatc cgggcgaatg
     1081 ggattgatcc ggggagtgct cccctggatg agcaatcggc ggtaaaaatt ctcatgcgac
     1141 ctgtgcccgg ggatcaggag ctggagcttt ccggcgtcac ggctgtaccc tggttttgga
     1201 ttgtggcagg agcgatcatt gcttcagcgg ggataatttt gctggtaaaa tatgtccaga
     1261 gatatttcaa gccccgcaga cccgaagacg aaaccgatta aaccttcagg agatgctgtg
     1321 ggtattaatg ttagcggcaa gtgaacaagg tcgcatgtcc gatttgtctg accgagagtt
     1381 gatcctacaa ctccaacagg gtagtttaga ggccctggga gagctctatg accattttcg
     1441 gcagatggtc tatcggactg cgctggcgat caccagcgac ccggaagctg ccaatgattt
     1501 gctgcaagac gtctttttac gcttatatcg ttttgcagat cgcatcgacc cgcagcgccc
     1561 gattgaaccc tggctttacc ggatgacagc caacctggcc tatacctggg tgaagcggcg
     1621 caatcgctgg gtccgtccgc tcgaggatct ggcggattgg ctcgcaggaa gcagccgcaa
     1681 ctcaccgcag gagactgcgg agatgcacga tgattggcag cgtgtccagc aggcaatcgc
     1741 tggactgccc ttaccccaac gcatggtggt cgtattgtat tatttgaatg atctgtcttt
     1801 acaagagatt tcggagattc tggatgtgcc cgtaggcacg gtcaaatctc gcctgcatta
     1861 tggacggaat tcgctcaaac gcagcctggg tctgcagggc gagacactgc cggattggaa
     1921 ttatgaaggt tcgtagggtt tagtttaaca tccggggatg gcgatctgat ggataattac
     1981 ttgggacggg tattgaaaaa ttgggcagcc agcagccagc ctcacgccaa tggtcgtgaa
     2041 cggctgctgg ctaatgctgc cagcgggcgc aaacccgaac agagaaaatc tgggcttgaa
     2101 acgatgcgca gcctgttatc cactccaaat tatcgtggtt tccacgagga aattcgcacc
     2161 cgaccgacct ctcaagcact catctggtcc ttccagatgg ttgcttcaac tcgcctcctg
     2221 gcttaatcag aatcctttat gtttcagcga ctgacaacta aatatatcct cctgctcgca
     2281 gccttactgg tcattggagc tctctcaagc tgtacggtcc aacctcaacc gacagcttct
     2341 cctgtttttc cgttacctga gagtccgacg cctgagttac gtgctttacc caccccctcg
     2401 ccgactcccg agaagcgagt gttgacgatc tgtacgcttc aggaaccagt ttcacttttt
     2461 atctacaacg accaatctca agcggcgcgc aatatccgcg aagccatcta cgacggaccg
     2521 gttgatatgc tgggctatcg accagcacca gtgatcctga ctgagcttcc cactcaggca
     2581 aatggtgggg tgattattga gagtttgaca gtctccgcgg gggcgaatat tgtcaatgcc
     2641 cagggagagc tggtcaatct gaaagatggg gtccaatatt atccggcggg atgtcgttca
     2701 accgagtgcg cgctgactta tcagggccag gagcccgccc agatggatca aatggtggtc
     2761 cggtatcacc tgcgtccagg cctgcagtgg tccgatggct cccctttaac cgcagacgac
     2821 tcgcagtatt cttttgaagt ggcccgtgag ctatatccgc gtgtccgcca aaacttaatc
     2881 acgcatacgg ctgtatacca ggccgaagat tcagaaacgg tcctgtggag aggtttgccg
     2941 ggctaccggg accctgctta tggcagtatc tttttccagc cactgccgcg ccatgcctgg
     3001 ggaagcctgc cagccgctga gcttctggca aatgaaggcg ccaatcgtgc tcccctgggc
     3061 tggggggctt atacggttgt cgagtggact gagggcgatc acatcacgct ggcgcccaac
     3121 cccctgtatt tccgcgcagc cgaaggatta ccttacttcg acaatctggt attccggttt
     3181 atatccgatc aagaccagat cctggcggga ttactggcgg gagaatgcga cctgatcgat
     3241 gatacggccg ggttggagac ccgtgccagc gagctgaacc agcttcagga agaaggtaaa
     3301 ctgctggctg cctacgaggt aggtactgcc tgggagcacc tggattttgg gatcgaagcc
     3361 tacgatccgg ctgcactggc aaaccccacc ctgttccaat ctccccagct gcgccaggcg
     3421 atcgccacct gcatcgatcg ggagcggctg gcgaatgagc tgtttttcgg gatttcggat
     3481 gtgccagtta cctatgtgcc tccaacccat cccctggcga atcccgacgc agcgcgctat
     3541 gcgtatgatc cccaggccgg gggcgccttg ttggaagcag ctggctggtt ggatccagac
     3601 cagaatccgg cgacaccgcg tcaggcccaa ggtgtgtcca ggatagtgga tggcacaccc
     3661 ctggcgatgg agctgctggc cagcaatgag ccggaaaagc aagctgcagc ggctatcctg
     3721 caaagctcgc tggcggaatg cggcatccag ctcgatattc gcttagttcc tctggaagaa
     3781 ttgtatagcc ccggtccaga aggtccggtt tttggtcggg cattccagct ggcgcagttc
     3841 aactggtcta attcttatga gccgccctgt tttctgtacc tttccagcga gatacccggc
     3901 ccctatccag agtacgcgcg cggctggggt ggggcaaatg ccagcggcta ccgcaacccg
     3961 gaatatgata ccgcctgccg gttggccatg aattctttac ccgaagaccc tgagtacgcg
     4021 gcgaatcacc tgcgcgctca ggagattttt gcgacggacc tccccgctat cccactgtat
     4081 atgcgcttga agctggtggc aggtcgccct gacctgtgtg aaatgcaggt cgttccgggg
     4141 gggagcagcg ctctctggaa tctggagtca ctcaattacg gcgagacttg cggtccctag
     4201 ctctaaagaa tttggcgact ttacaaagcg gttagttttt tcagacagca gggtggttat
     4261 aatacctgca tggcccgtta tcacatcctt attgtcgatg accaacgcga tgtgcgtgtc
     4321 cttctgcgca gcggattgga aaccttgcag cttgatctga aggttatcga cgtaccttct
     4381 gccgaagaag cgctgctggt cctgggaaaa gaaccgatcg acctgatggt ggcagacgtg
     4441 cggctgccgg gtatgtccgg cctggagctg cgggcgcagg ctggacggcg gatgccaacg
     4501 acccagctga tcctcattac ggggatgacg gatgaaaatg tgaagcagca gctctcacag
     4561 gccggtgtgg cagcttattt tttcaagcct atccagattg gcctgttttt agaagaagtc
     4621 tgccggtgtc tgggggtaaa accgggtagt tcagacaacc aggaaacagc tcgcccagaa
     4681 gttacgccgg cctcggttcg ccagacctcg tctaatcccc tgattgagct ctgcgtcagc
     4741 cagtccgtct caggttgctg gcttttgaca gggcggggga aaattctcta tgaatacggc
     4801 aggatcgcag cccagaacga agtcaaagaa tttaagaaga ccctcctggg tttattagcc
     4861 catgaccgcc agcaggtcca tgcacagtca ggaattacgt ttccatgcca tcacctgttt
     4921 ccgggtcagg gacgcgattt gtgggttacc agtctgggtt ttgaccttgt cctggcgttc
     4981 gccttgccgg caggcgaggt agtgcaccag gcggctggaa tgctgcgcga gatcgggaag
     5041 ctgcacgcca gcctggcagt ccgcgaaccg gagaagggta aacacccggc tgcagtgaaa
     5101 tctgccggtg aggtgtctgg ggacaaggag ctgagcgcta tcctgggaca gggttcaaaa
     5161 cccgggatgc gcgctgagga attggatgct tactgggat
//
LOCUS       DATMXK010000258         4668 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_167456, whole genome shotgun sequence.
ACCESSION   DATMXK010000258 DATMXK010000000
VERSION     DATMXK010000258.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4668)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4668)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4668
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_167456"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..1199)
                     /locus_tag="VN363_04740"
     CDS             complement(33..1199)
                     /locus_tag="VN363_04740"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83849.1"
                     /translation="MRPPISDLGLMAELLAITAMISAAAGYMAYRFGWMDRSPAIRST
                     LIGGYLLSSLLTLLNVGVTAWLMFTSLHDLYLAMVLLVFAGGIAIVFGVYYTSALSNR
                     IKNLDLIARDLAQGNLQARAPTSGRDEIAALGNTFNDMAQQLQAAAEKQRQIDLLRRD
                     LIAWVGHDLRTPLASVRAVLEALADGVIEDPESVQRYLGTARRDINALSELIDDLFEM
                     SQIDAGGLKLNREDSSLTDLISDTLESFSELAARQKVTLEGQAGPNLDPVWMDTLKIG
                     RVLNNLVSNALTYTPPGGTVRLIAQRRSREIEVMVEDNGSGIAPQDLPTIFDRFTRGE
                     KSRSRSTGGAGLGLAIARGIVEAHGGQIKVESAPGQATHFSFTIPNRQKDGTNR"
     gene            complement(1321..2010)
                     /locus_tag="VN363_04745"
     CDS             complement(1321..2010)
                     /locus_tag="VN363_04745"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83850.1"
                     /translation="MNPALIYIVEDEPSIGEVVGIYLRRAGFQVLGVTNGQAALEALD
                     RQFPDLMILDLMLPGLDGLEITRRVRDRSDLPIIMLTARREEVDRIAGLEMGADDYVI
                     KPFSPQELVSRVRAVLRRTRPSSPDAPEAPLVFENLRIDPATRLVHVKDQEINLTAKE
                     FEMLWYLARHPRQVFSRDQLLENVWGLSEYIDPGTVTVHVRRLRQKIETDPANPHFIQ
                     TVWGVGYKFEP"
     gene            2239..3636
                     /locus_tag="VN363_04750"
     CDS             2239..3636
                     /locus_tag="VN363_04750"
                     /inference="COORDINATES: protein motif:HMM:NF012457.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="family 1 glycosylhydrolase"
                     /protein_id="HWQ83851.1"
                     /translation="MPNLPLQTKITGYIPSALIKEMDMAQANLMFPRGFLWGSATASH
                     QVEGQNTNNNWWAWEQQAGRIINGDKSGMACDWWGGRWREDFDRAAEAHQNAHRLSIE
                     WSRVQPAPDRWDESALDNYREMLQGLTERGMTPMITLHHFSDPIWLTERGGWESPDSA
                     VLFNRYVEKTVEALSEYVNLWITINEPNTLLVSAFLFGSFPPGKQDRGSAIRAAENFA
                     RAHVLAYHTIHRLQVQARVGIALNYRSLVPARSWSPLDHWAANTYRGLFNDLFPDAFQ
                     TGRLRIPFHSLHIPQAKGTQDFLGINYYTRDQVKFNILKPDDLFANRSFLPDAELSPT
                     GFISNVPEGMFEALKWGMKFNLPMIVTENGVEDADDHLRPRYLIQHLHQVWRAVNFNF
                     PVKGYFHWSLVDNFEWERGWTQRFGLWELDIETQARRKRSSADLYSEICRTNALSSDL
                     VARYAPEVLPLLFAD"
     gene            3660..4547
                     /locus_tag="VN363_04755"
     CDS             3660..4547
                     /locus_tag="VN363_04755"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ83852.1"
                     /translation="MASLLITDPILVCRPALAQDTPAMWEITRTTFDGHDYVPHEWDQ
                     WLREASGCLAVVELGGKVVGLARLTRFGERDWWNQALRVHPDFRGQGVASHLNKYLFE
                     RWMQIGSGTLRFTTTADRYPVQHLAEQTGFVRVGEFTAFIAPAIKEQPDQFTLISREQ
                     SGDALDFIQQSPLLDWQYGLLNWGWEWTTPELQWIQKAIEKRHAWWWKDGQGLILAYV
                     DKDDQVNEESLAVELLAGTPETAVDMLVDFRRLAAGEGYADALWTASLQDRLAPVLAQ
                     AGYIRAWDNSVYLYAIKHS"
BASE COUNT         1115 a         1182 c         1272 g         1099 t
ORIGIN      
        1 atatattaca gaaagctaaa cgctggatga atttaccggt ttgttccatc cttttggcga
       61 tttgggatcg tgaaagagaa gtgcgttgcc tgaccaggcg cactctcgac cttaatctgc
      121 ccgccatggg cttcaacaat gccgcgcgca atcgccaatc ccaggccggc gccaccagtt
      181 gaccggctgc gggatttttc gccgcgagta aagcgatcaa aaatggtggg taggtcctgt
      241 ggcgcgatac cagagccatt atcctcgacc atcacttcaa tctcgcggga tcgccgctga
      301 gcgattagcc gtacggttcc gccgggaggg gtgtaggtga gggcattgct gaccaggtta
      361 ttcaaaactc gcccaatctt cagggtatcc atccagactg gatcaaggtt gggtccagcc
      421 tggccctcca gagtgacttt ctggcgtgct gcaagctcgg aaaaactctc cagggtatcc
      481 gagattaagt ctgtgagcga gctgtcttca cggttaagct tgagcccgcc ggcatcgatc
      541 tgagacattt caaacaggtc atcgatcaat tccgaaagcg cattgatgtc ccggcgagcc
      601 gtgcccagat agcgttgaac cgactcaggg tcctcaatga ccccatccgc gagcgcctct
      661 aacactgccc ggaccgatgc gagtggagta cgcaggtcat gccccaccca ggcgatcagg
      721 tcccggcgta acaggtcaat ctggcgctgt ttttcagcgg cagcctgtaa ttgttgggcc
      781 atatcattga atgtattccc cagtgcagct atctcatccc ggcctgaggt tggcgcccgc
      841 gcctgcaggt ttccctgagc caggtcgcgg gctatcagat ccagattttt aatccggttg
      901 ctgagggcgc tggtgtaata caccccaaag acgattgcaa tccccccggc aaacaccagt
      961 aataccattg ccagatagag atcgtgcagg ctggtgaaca tcagccaggc tgtcacgccg
     1021 acgtttaata atgttagcaa gctggagagc aagtaaccac caatcaatgt cgagcggatt
     1081 gcaggtgagc ggtccatcca cccgaaccgg taagccatat agccggcagc agcagagatc
     1141 atggctgtaa tcgccaatag ctcagccatc aaccccagat cactgattgg ggggcgcata
     1201 acgctgtaaa aaatccccag gcttatggca acgatcagga atatacctgt cagatatcct
     1261 gttttctgat caggagattt caacgcaatt ttggaagttt gaagagggtt cataagatca
     1321 ctaaggctca aacttatatc ccacgcccca tacggtttgg ataaagtgtg gattggctgg
     1381 atcagtctcg atcttctggc gcagacgccg gacatgcact gtcacagtcc cgggatcaat
     1441 gtactcggac agcccccaaa cattttccag tagctggtca cgactgaaca cctgccgcgg
     1501 gtggcgggct aaataccaga gcatttcgaa ttctttggct gtcagattga tttcctgatc
     1561 ttttacatgg acgagccggg tggctggatc aatgcgcaaa ttttcgaaaa ctaatggggc
     1621 ttcgggtgcg tcaggcgagc tggggcgcgt ccgccgcaaa accgcccgca cccgactcac
     1681 cagctcctga gggctgaagg gtttaataac atagtcatct gcgcccattt ccagacctgc
     1741 aatccggtca acctcttccc gtcgcgccgt gagcatgata atcggtaagt cgctgcgatc
     1801 gcgcaccctg cgggtaattt ccaagccgtc cagccctggg agcattaaat ctaagatcat
     1861 taagtcagga aactggcggt ccagggcctc gagtgcagcc tgaccgttgg ttaccccgag
     1921 cacttgaaaa cctgcgcggc gcagataaat acctacgact tcgccgatgc tcggttcatc
     1981 ttctacaata tagatcaagg ctggattcat aaagattctc cgcgaagaac tatacactcc
     2041 ctcgattaat tgctggcgaa gaaatgatta cacattgatt acatctgagc tgtatcagac
     2101 atagcatatc agaattctcg gatagaggat aacatgaatc attcgagcgc gctgaggatc
     2161 actgttttcg tgcgctttgc tggaagatac ggctgaagat gatcgtctgg atgcatgcgc
     2221 tgctggtaaa tcgttgattt gccaaatctc ccgctgcaga ctaaaataac cgggtacatc
     2281 ccgagtgcat tgatcaagga gatggatatg gcccaggcaa atttaatgtt tcctcgcgga
     2341 ttcctttggg ggagcgcgac agcctcacac caggtggaag gtcaaaacac gaacaataat
     2401 tggtgggcct gggagcagca ggctggcagg ataatcaatg gggataagtc cggtatggcc
     2461 tgcgattggt ggggagggcg ctggcgggag gactttgacc gggcggctga agcacatcaa
     2521 aatgctcacc gcctatcgat agaatggagc cgggttcagc ctgcgcccga tcgctgggac
     2581 gagagcgccc tggataatta tcgtgagatg ctgcaagggt tgaccgagcg cggaatgacc
     2641 ccgatgatca ccctgcacca tttcagtgat cctatctggc tgacagagcg agggggttgg
     2701 gaatcaccag attcggctgt gctatttaat cgttatgtgg aaaaaactgt cgaagccctg
     2761 agtgaatatg taaatctatg gataacgatc aatgagccaa atacgctgtt agtgtccgcg
     2821 tttctgtttg gctccttccc acccggcaaa caggatcgcg gctcagctat cagagctgcc
     2881 gagaattttg ccagagcgca tgtcctggca taccatacga tccaccgttt gcaagtacag
     2941 gcacgggtgg gcatagcctt gaattatcgc agccttgttc cggcccgaag ctggtccccg
     3001 ctggatcact gggcagcgaa tacgtatcga ggattgttta atgatttatt tcccgacgct
     3061 tttcagactg ggcgactcag gattcctttt cattcgctcc atattcccca ggctaaaggc
     3121 actcaggatt tcttagggat caattactac acccgggatc aggtgaaatt taacatattg
     3181 aagcctgatg acttatttgc caaccgttcc tttttgcccg atgcagaact aagtccaacc
     3241 ggtttcatct ccaatgtccc cgagggtatg tttgaggcgc ttaagtgggg gatgaaattt
     3301 aatttaccca tgatcgttac agaaaacggt gtcgaagatg cggatgatca tttgcgacca
     3361 cgctatttga tccaacatct gcaccaggtc tggcgagcgg tgaactttaa ttttccggtt
     3421 aaaggctact tccactggtc tctggtcgat aattttgagt gggagcgagg gtggacgcag
     3481 cgctttggat tatgggagct cgatattgag acgcaggctc gccggaagcg gtccagtgca
     3541 gatttgtaca gcgagatatg ccgtaccaat gcactatcct cggatctggt tgcacggtat
     3601 gctcctgaag tacttccctt gttatttgca gattgaccca cactgattcg aggcaaacta
     3661 tggcaagtct attgatcacc gatccgatcc tggtatgtcg cccggcgctg gcgcaagata
     3721 cacccgccat gtgggagatc acgcgcacga cctttgatgg gcatgattac gttccccatg
     3781 aatgggatca atggctgcgg gaggcttcgg gctgcctggc agtggttgag ctgggtggta
     3841 aggtagtagg cctggcgcgc ttaactcgtt tcggcgaacg agattggtgg aaccaggcgc
     3901 taagagtgca cccagacttc cgtgggcagg gagttgccag tcatctgaat aaatatttat
     3961 tcgaacgctg gatgcagatt ggcagcggca cattgcggtt taccaccacc gccgatcgct
     4021 atcctgtgca gcatctggca gagcagacag gctttgtgag agtaggggag tttactgcgt
     4081 ttatcgctcc cgcaatcaag gaacagcctg accagttcac cctgatttcc agggaacaat
     4141 ccggggatgc tctggatttc atccagcaga gccccctgct ggattggcag tatggattgt
     4201 taaactgggg ttgggaatgg accacacccg agctgcaatg gatccagaag gcgatcgaga
     4261 agcggcatgc ctggtggtgg aaagacggtc agggacttat cctggcttat gttgataaag
     4321 atgaccaagt aaacgaagaa tccctggctg ttgaactgct ggctggaaca ccggaaactg
     4381 cagtagatat gctggtcgat tttcgccggc tggcagctgg cgaaggatat gcagatgctc
     4441 tgtggacagc ttccctgcag gacaggctgg cacccgtgct ggcacaggcg ggatacatac
     4501 gtgcctggga taattcagtt tatttatatg ccataaaaca ctcgtagtgc ctcaaaataa
     4561 aaggttactc tagggataac gatgcctcat atagaaaggt taccgaatga tctcccacag
     4621 cctggctaca ttctggtcaa tcttgttgcg caaggccaca gggttgag
//
LOCUS       DATMXK010000259         2116 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_77464, whole genome shotgun sequence.
ACCESSION   DATMXK010000259 DATMXK010000000
VERSION     DATMXK010000259.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2116)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2116)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2116
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77464"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..467
                     /locus_tag="VN363_04760"
     CDS             <1..467
                     /locus_tag="VN363_04760"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ83853.1"
                     /translation="HTYAGNPPIEALRGINLRIFPGEYVAIIGANGSGKSTLARHLNA
                     LLLPGRGRVLAAGIDTQDTDQRSAIRRVVQMVFQQPDSQIVATTVEEDVAFGPENFGI
                     RDGDARPRAVCTGDGGNVGAAQTPAAPLIGGSEAARGDCGSAGAQAAGARIR"
     gene            352..1701
                     /locus_tag="VN363_04765"
     CDS             352..1701
                     /locus_tag="VN363_04765"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ83854.1"
                     /translation="MGMWELRKRPPHRLSAGQKQRVAIAGALALKPRALVLDEATSML
                     DPAGRRTVVEVLRLLQAQGTTLVTITHEMEEAAEANRVVVLQRGQVMMDGDPHAIFAR
                     AAELRELGLDIPPLADLSLRLGLPVCLTVDELLAALGPAPAAMQTLLENTPCSPSPQP
                     LTETITDPIVTVEGLQHAYLRGTPLESPALHGVDLQIPRGEITGLIGPTGSGKSTLMQ
                     HLNGLIRPQAGRVVVDGKNWADPELDVGAARQKVGLLFQQPEDQLFERYVGDDVAFGP
                     RQLKLEAAEVRQRVRDAMDAVGLPFESFKDRLTQTLSGGEQRRAALAGLLAMQPRLLV
                     VDEPTAGLDPHGRAEAQAIFRRINQAGVTVIFGSHRMEDVLALCKQVVALREGRVART
                     GPVREVLGRPDIDMPVLPLVLAASRLCEAGWPVPVTALTVPEIADVIIKAGFQVAAG"
     gene            1719..>2116
                     /locus_tag="VN363_04770"
     CDS             1719..>2116
                     /locus_tag="VN363_04770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83855.1"
                     /translation="MEDLELMRNITIGQYLPTGSAVHRLDPRTKLLGIGLLILSIITI
                     KSVGGLLALLVMIIGLVLLARVPLGFALGGIRPALPILIIIAILQLLFGWGAPEGMVC
                     PSLWSFWILKITGCSILSVIGMILRLVALIL"
BASE COUNT          358 a          618 c          721 g          419 t
ORIGIN      
        1 ggcataccta tgccggtaat ccgcccatcg aggcgctgcg cgggatcaat ttgcgcatct
       61 ttcctggcga gtatgtggcg attattgggg ccaatggctc ggggaagtcg accctggcgc
      121 gccatctcaa cgccttgctg ctacccggtc gcgggcgagt gctggcggcg gggatcgaca
      181 cccaggatac agaccagcgc agcgcgatcc ggcgggtggt gcagatggtg ttccagcagc
      241 cggattcgca gattgtggcg accacagtcg aagaagatgt cgcctttggg ccggaaaatt
      301 ttggcatccg agacggagat gctcgtccac gtgcagtctg cactggagac ggtgggaatg
      361 tgggagctgc gcaaacgccc gccgcaccgc ttatcggcgg gtcagaagca gcgcgtggcg
      421 attgcgggag cgctggcgct caagccgcgg gcgctcgtat tagatgaagc gacctccatg
      481 ctcgacccgg ccggacgccg gacggtggtg gaagtgctgc gtctcttaca ggcccaggga
      541 acgaccctgg taacgatcac ccatgaaatg gaagaggctg ccgaggccaa ccgggtagtg
      601 gtgctgcagc gcggccaggt gatgatggat ggcgatccgc atgcgatctt tgcccgcgcc
      661 gccgagctgc gggagctggg gctggatatc ccgcccctgg cagacctgtc cctgcgcctg
      721 gggctgccgg tgtgcctgac ggtggacgag ctcctggctg ctctgggccc tgccccggct
      781 gccatgcaaa ctctgctgga aaacacaccc tgcagcccga gtccccagcc actaacggag
      841 acgatcactg atccgatcgt gacggtcgag ggtctgcagc atgcgtatct gcgcggcacg
      901 cccctggaat cgcctgccct gcatggggtg gatctgcaga tcccccgcgg agagatcacc
      961 ggactgatcg gcccgaccgg ttcaggtaaa tcgactctca tgcagcattt gaacggcttg
     1021 atccgaccgc aggcaggcag ggtcgtggtg gatggcaaaa attgggccga tccggagctg
     1081 gacgtgggcg ccgcccgcca gaaagttggc ttgctcttcc agcagcctga ggaccagctc
     1141 tttgagcgct atgtgggcga tgatgtcgct ttcgggccgc gccagttgaa gctggaggca
     1201 gccgaagtgc gccagcgggt aagagatgca atggacgcgg tcggactgcc ctttgagagc
     1261 ttcaaagatc gcctcacaca aaccttatcc ggcggcgagc agcgccgggc agctttagca
     1321 ggcttgctgg cgatgcagcc caggctgctc gtggtggacg agccgacagc cgggcttgat
     1381 ccgcatggtc gtgccgaagc ccaggcgatt ttccgccgca tcaatcaggc cggggtcacg
     1441 gtgatctttg gctcgcaccg catggaggat gtgctggcct tgtgtaagca agtggtggcg
     1501 ctgcgcgagg ggcgggtcgc gcgcacaggt ccggtacgtg aagtgttggg ccgcccggat
     1561 atcgatatgc ccgtcctgcc gctggtgctg gcagcctccc ggctgtgcga ggcaggctgg
     1621 ccggtcccgg tcacagcgtt gactgtccct gaaattgcag acgtaattat taaagctggt
     1681 tttcaggtgg cggctggcta gccgggcgtg gaggtaaggt ggaagatctg gaattgatgc
     1741 gcaacatcac catcgggcag taccttccca ccggctctgc cgtacaccgc ctggacccgc
     1801 gcaccaagct tctggggatc ggcctgttga tcctctcgat cattacgatc aagagtgtgg
     1861 ggggccttct ggcgctgctg gtgatgatta ttgggctggt gctgctggcg cgcgtcccgc
     1921 tgggtttcgc cctgggcggc atacgcccgg cgctgccgat cctcattatt atcgccatcc
     1981 tgcagctgct gttcggttgg ggcgctccgg agggcatggt ctgcccatcg ctgtggagtt
     2041 tctggattct caagatcacc ggctgctcga tcctgtcggt gatcggaatg atcctgcgtc
     2101 tggtagcttt aatcct
//
LOCUS       DATMXK010000260         3611 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_31182, whole genome shotgun sequence.
ACCESSION   DATMXK010000260 DATMXK010000000
VERSION     DATMXK010000260.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3611)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3611)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3611
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31182"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1540
                     /locus_tag="VN363_04775"
     CDS             <1..1540
                     /locus_tag="VN363_04775"
                     /inference="COORDINATES: protein motif:HMM:TIGR03296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="M6 family metalloprotease domain-containing
                     protein"
                     /protein_id="HWQ83856.1"
                     /translation="DEPDGYIDTFQSIHAGIGNESCSPACDDYAIWSHSWYAFSTGAG
                     PDGPGPDFNPDGGVRIGDSNLWVGKYTIQPENGGVGVFAHEFGHDLGLPDLYDTSGGE
                     NGTAFWTLMSSGSWVDTGQDTIGNAPSHMGAWEKFQLGWLNYEVAYAGQYSDHKLGPM
                     EANTKQAQGLFVVLPDKPVTINIGTPRYGASYYYSGSGDNLDNFMFKAFSLPAGATLS
                     AKVRYQIESNWDYAYLVYSTDGGATWTSLETNRSTTTDPNGQNFGFGITGSATSSSWF
                     DLTASLPEGDVLLGFRYWTDSNTGGFGFMVDEINITGQPVDGAEADAGWTFAGFRITS
                     GQEVQNFAHYYVAEYRAYWGYDKNLQFGPYNFGFLDNPLLGDWVEHLPYQDGLLIHYW
                     DTSQRNNNTRQHPGQGLLLPIDAHPTAMIRADGGVWRNRMQTYDSTFTLAPTDPLTLH
                     YLSQPSYHPSLPGVPVFDDRNSYYDPLNPQGSVIVPNTGTQIRIQSISAQGNFMQVQV
                     RPAK"
     gene            1739..3007
                     /locus_tag="VN363_04780"
     CDS             1739..3007
                     /locus_tag="VN363_04780"
                     /inference="COORDINATES: protein motif:HMM:NF017760.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ83857.1"
                     /translation="MGHLAAHLRQFSRETFSSLQIRNYRLYYIGQIISTSGTFMQSVA
                     QAWLVLKLTNSGPALGLTTALQYLPILFLGPYGGVIADRFSKRKILTITQSISGVLAL
                     ILGVLVATNLVQVWMVYILAFCLGMVNVFDNPTRQTFVMELVGADHIRNAVTLYSTLV
                     NLARIIGPAIAAALIAAFGLAPCFLINGFSFIAVVIMLGLMDPSQLITSPPAPPARGQ
                     LQEGIRYVLSTPILATTLVMLAIIGTLTFEFQVSLPLLVQFTFKGDASSYAFLTSAMG
                     FGAATGGIFFASRKGITPSKLVSAAMLFGLAVLAAAWMPSLLLTGLVMVLVGVFMINF
                     TSLGNSILQITSSSQMRGRVMALWSVAFLGSTTIGGPIVGWFAEAAGPRWGLALGGLA
                     ALAAAAFGLFNLRKMQLTEPATAGAEKRPV"
     gene            complement(3308..3520)
                     /locus_tag="VN363_04785"
     CDS             complement(3308..3520)
                     /locus_tag="VN363_04785"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ83858.1"
                     /translation="MDHEGHETGERHARGRVVGRYAELRKYFEQLASGGMVAMPLEKA
                     PWGDTFGMLTDQFGINWMVNIVGQAG"
BASE COUNT          672 a         1203 c          934 g          802 t
ORIGIN      
        1 cgacgagccg gatggctaca tcgatacctt ccagtcgatt cacgccggca taggcaatga
       61 atcttgctcg ccggcctgcg acgactatgc catctggagc cacagctggt atgccttctc
      121 cactggcgcg gggccagatg gtccgggccc ggacttcaat ccagatggcg gtgtccgcat
      181 cggcgacagc aatttatggg tgggcaagta caccatccag cccgagaacg gcggcgtggg
      241 cgtcttcgcc catgagtttg ggcacgatct gggcctgcca gacctgtacg acacctccgg
      301 tggcgagaat ggtactgcct tctggacgtt aatgtcctcc ggttcctggg tggacaccgg
      361 gcaggatacc atcggcaacg cccccagcca catgggcgcc tgggagaaat tccagctcgg
      421 ctggctgaat tacgaagtgg cttatgccgg tcaatattct gatcacaagc ttggcccgat
      481 ggaggccaat accaaacagg cccagggcct gtttgtggtc ctgccagaca agccggtcac
      541 gatcaacatc ggtacgccca gatacggcgc cagctactac tacagcggct cgggcgataa
      601 tctggacaac ttcatgttta aggctttctc gctgccggcc ggggcaaccc tttccgcaaa
      661 ggtgcgctac cagatcgaat ccaactggga ttacgcctac ctggtgtact ccaccgatgg
      721 cggggcaacc tggacctcgc tcgagaccaa ccgctccacc accaccgatc ccaacggcca
      781 gaattttggc ttcgggatta ccggcagtgc caccagcagc agctggtttg acctgacggc
      841 cagcctgcct gagggcgacg tgctgcttgg cttccgctat tggaccgact ccaataccgg
      901 tgggtttggt tttatggtcg atgagatcaa tattaccggc cagcccgtcg atggcgccga
      961 agcggatgcg ggctggactt ttgccggatt tcgcatcacc agtgggcaag aagtccagaa
     1021 ttttgcccac tactatgtgg ctgaataccg cgcctattgg ggatacgata aaaacctgca
     1081 gttcggacct tacaactttg gcttcctgga taacccgctg ctgggcgact gggtcgagca
     1141 tttaccctac caggacggac tgctgattca ttactgggat acttcccagc ggaataacaa
     1201 tacccgccag catccaggtc agggcctgct cctgcccatc gacgcccatc ccaccgccat
     1261 gattcgggcg gatggcggcg tctggcgcaa ccgcatgcag acctatgact cgacctttac
     1321 cctggctccc accgatcccc tcaccctgca ctatctgagc cagccgtctt accaccccag
     1381 cctgcccggc gtcccggtct tcgatgatcg caattcgtat tacgatccct tgaacccgca
     1441 gggcagcgtg atcgtgccga acaccggcac ccagatccgc attcagagca tcagcgccca
     1501 gggcaacttc atgcaagtcc aggttcgccc ggcgaagtaa attctgccgt tttgctccat
     1561 gctccactcg gcgtaacaaa aagagcccgc cttcctggcg ggctcttggc tggataccgc
     1621 tatctgaaaa cagctggcag taaacagctg gctgtatgca gctagctgta aacagttggc
     1681 gctgtaattc agcaagaagt tagataatag tataattccc ctcatccacc ggaatctcat
     1741 gggacatcta gccgctcacc tccgccagtt cagccgcgag accttctcgt ctctccaaat
     1801 ccgtaactac cgcctgtatt acatcggtca gatcatctcg acctccggca ccttcatgca
     1861 gtcggttgcc caggcctggc tggtgctcaa gctgaccaat tccggccctg ccctcggcct
     1921 caccaccgct ttgcagtacc tgcccatcct gtttctcggt ccgtatggcg gtgtcatcgc
     1981 cgaccgcttt tccaagcgca aaattctaac catcacccag tccataagcg gcgtcctggc
     2041 attgatcctg ggcgtgctcg tggctaccaa tctggtacag gtctggatgg tctatatcct
     2101 ggccttttgc ctgggcatgg tcaacgtctt tgacaacccc acccgccaga ccttcgtgat
     2161 ggagctggtt ggcgccgatc acatccgcaa cgcggttacc ctgtactcga cgctggtcaa
     2221 cctggcccgc atcatcggtc cggcgattgc cgcagccttg atcgccgcct tcgggctggc
     2281 tccctgcttc cttatcaatg gattttcttt tattgcggtg gtgatcatgc tgggcctgat
     2341 ggatcccagc cagctgatta cttcaccccc tgcgccgcct gccaggggcc agctccagga
     2401 gggcatccgc tacgtgctct ccacccccat tctcgccacc accctggtga tgctggcgat
     2461 catcggcacg ctcacctttg aatttcaggt cagcctgcct ttgctggtcc agtttacatt
     2521 taaaggggat gccagcagct acgctttcct gacttctgcg atgggattcg gggccgccac
     2581 cggtgggatc ttcttcgcca gccgcaaagg catcacccct tcgaaactgg tcagcgccgc
     2641 catgctgttc gggctggcgg tcctggcagc cgcctggatg cccagcctgc tgctcaccgg
     2701 cctggtcatg gtgctggtgg gagtgtttat gatcaacttc acctccctcg gcaatagcat
     2761 cctgcagatc accagctcgt cccagatgcg cggccgggtc atggcattat ggtcggtggc
     2821 attcctgggc tccaccacca tcgggggacc catcgttggc tggtttgctg aagccgccgg
     2881 accgcgctgg gggctggcgc tgggcggtct ggcggccctg gctgccgccg ccttcggcct
     2941 gttcaacctg cgcaagatgc agctcaccga acctgccact gctggcgctg aaaaaaggcc
     3001 ggtttgaccc gagtcaggct ccgtagcagg gcagcagcac gcccgtacag gtttccgcgt
     3061 gccgccagcc ggtccaggtc accatccagc gccaggcaaa tgtcccttca gattgccgga
     3121 gaacctgtcc ggtctgcccc tgatccgtag acgaccgtac aggttttaag gcacccgccg
     3181 cttcaggctg ccttaaaacc tgtacggtct gcccacaaaa tggctaaccc tgcaccttca
     3241 acagcctcct gttagtttct tctgctctcc cctgcgcctg tctgcgtttt ctgcggacaa
     3301 agcgctccta ccccgcctga cccacgatat tcaccatcca gttaatccca aattgatccg
     3361 tcagcatccc aaacgtatct ccccagggag ctttctccag cggcatcgcc accatccccc
     3421 cgctcgccag ctgctcaaaa tacttccgca gctcagcata ccttccaacc accctgcctc
     3481 ttgcgtgcct ttcgcctgtt tcgtgccctt cgtggtccaa tcacttaacg tgcaggctgg
     3541 cttatcctcc gtctcattcc caattcgttc aattccttcc caacccgtga tctccacggg
     3601 cattcgttgc c
//
LOCUS       DATMXK010000261         3849 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_57395, whole genome shotgun sequence.
ACCESSION   DATMXK010000261 DATMXK010000000
VERSION     DATMXK010000261.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3849)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3849)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3849
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57395"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1000)
                     /locus_tag="VN363_04790"
     CDS             complement(<1..1000)
                     /locus_tag="VN363_04790"
                     /inference="COORDINATES: protein motif:HMM:NF017743.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TRAM domain-containing protein"
                     /protein_id="HWQ83859.1"
                     /translation="MSESETFEIEFTGLIYGGDAIGRLPDGKAVFVPFVLPGETARIR
                     LVDQRRGFARGQFVELLQPSPARIEPRCAHFGECGGCHYQHLPYENQLAAKAAILTDQ
                     LQRIGGLQSVPQLAPVPSLEPFNYRNYFQFHLAPDGRPGFYRAQSTTILPIQECHLPQ
                     PPLAAVWPQMEFEAGTGIDRVGLRCGVAGEVQLILESDDPRLPEFSLEELDLTAVYLS
                     PAGTSVLAGGSSLPMEILGREFQVSAASFFQTNPAVAERMVEHVLASLKLDPGDSVLD
                     LYCGVGLFSAFLAPRCAELVGIESSPSAVEDFAANLDEYDHVSIYEATAEQALPALD"
     gene            1079..2626
                     /locus_tag="VN363_04795"
     CDS             1079..2626
                     /locus_tag="VN363_04795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83860.1"
                     /translation="MRKLQFGALVVLILLACRALQPGVLFGEFPATATMTPAQAVTAA
                     PVLKATPTRDAAGPPLSPEIVEEEFQVVTHPDGALYAGDRVSFEIIPGADFELRGQQA
                     RVRLVSPFVQEIGEVEFGRHGIGQRAQATLTWAWDTRDLQPGSYELEFTILPQGKVWR
                     QTLDLLPAGERPQPEAVWKEAQLDCCVVDYISGTEAERDLDRLLEIIEEQSRLVSEQM
                     GVSLEQPVKVTLVPRVLGHGGFAGEEITISYLDRLYTASNLEMIFHHELVHIFDSQLG
                     GDLRPTMLVEGLAVYLSGGHFKPEPILERAAVLLPAEPGCIVCGMGEYVPLAKLVDDF
                     YSSQHEAGYLQAAALVEFLVESYGWEGFNAFYRDIHPDPEGSQAESMEASLQAHFRLS
                     LAELDEQFQSRLRQVRLDPKWVEDVKLSVEFYDAVRRYQERFDPSAYFRTAWLPDTAQ
                     MQAEGITADFIRRPQQAVNIILENLLTAVGEALKAGQYHQGLEHLETVQYLLDHLPGE
                     INTAQAN"
     gene            complement(2680..3339)
                     /locus_tag="VN363_04800"
     CDS             complement(2680..3339)
                     /locus_tag="VN363_04800"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWQ83861.1"
                     /translation="MAESSYRLVMRTGPNPGQTFDLLQPEITIGRDINNTIVINDAEI
                     SRKHAHVYIQGGFYQIEDTGSTNGTFVNGQRLMGPHLLRPGEVIFLGETVSLAYEFSQ
                     FDPNATVASVPRGVPEPAIPAAQPAPQPVVNYPPPAYTPPAPPAPVYTGQVPPGPGEP
                     VYEEEYEEKQTNRTRWVVGVGCLLVLVCIVGVGLLAFDTLNLYCVSPFDSLSGLFYSC
                     P"
     gene            3667..>3849
                     /locus_tag="VN363_04805"
     CDS             3667..>3849
                     /locus_tag="VN363_04805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Clp protease N-terminal domain-containing
                     protein"
                     /protein_id="HWQ83862.1"
                     /translation="MNLEKYTQKSQEAILAAQRMAQDYNHPSIEPAHLLLALLRQEDG
                     VVPAVVTRVAGSVLALR"
BASE COUNT          870 a          998 c         1244 g          737 t
ORIGIN      
        1 tgtcgagggc gggcagggct tgctctgcgg tggcctcata aatggagaca tgatcatact
       61 catccaggtt ggcggcgaaa tcttccacgg cgctgggaga gctttcgatg ccgaccagct
      121 cagcgcagcg cggggcgagg aaggcgctga acagcccgac cccacaatag agatccagga
      181 ccgaatcgcc gggatcaagc tttagagagg ctaaaacatg ttcgaccatg cgctcagcaa
      241 cagcaggatt ggtctggaag aaggaggcgg cagagacctg aaactcccgc cccaggattt
      301 ccatgggcag gctgctgccg ccggctaaaa ctgacgtacc agccggactg agatagacgg
      361 cggtcagatc cagctcttcc agactgaatt caggcaagcg ggggtcatca ctttccagaa
      421 tcagctggac ttcgccggcg actccgcagc gcagacccac gcggtctatc ccggtgccgg
      481 cttcgaattc catctggggc cagactgcag cgagaggcgg ctggggcagg tgacattcct
      541 ggatggggag gattgtggtg gactgggcac ggtaaaaacc cgggcgacca tccggggcga
      601 gatggaactg gaagtaattg cggtagttga aaggctcaag ggatgggaca ggcgcgagct
      661 ggggaacgga ctgcagcccg ccgatgcgct gcagctggtc ggtgaggata gcggctttgg
      721 ctgccagctg gttctcgtaa ggcaggtgct ggtaatggca gccgccacat tcgccaaaat
      781 gggcgcagcg cggctcaatc cgggcggggg aaggctggag cagctcgacg aactggccgc
      841 gggcaaagcc ccggcgctgg tccaccaggc ggatgcgggc tgtctctcca ggcaggacaa
      901 acggcacaaa gaccgctttg ccatctggca ggcggccgat ggcgtcgccg ccataaatga
      961 ggccagtaaa ttcgatctca aaagtttcgg attcagacat tgcgtagaat tcccaggtgt
     1021 aaggggcagg agcttcccca gcaggacgac gcccaatttt accagctata atgttggtat
     1081 gcgtaaactg cagtttgggg ccctggtggt attgatcctg ctggcatgcc gtgcactcca
     1141 gcctggcgtg ctgttcggag agttccccgc cacggcgacg atgacgcctg cccaggcggt
     1201 gactgccgca ccggtcctga aagcgactcc gacccgggat gcagctggtc caccactcag
     1261 cccggagatt gtcgaagaag aattccaggt tgttacccac ccggatggag cgctgtacgc
     1321 cggtgaccgg gtgagctttg agattatccc cggcgccgat tttgaactcc gcggccagca
     1381 agccagggtg aggctggtta gcccattcgt gcaggagatc ggggaggtcg agtttgggcg
     1441 gcatgggatt ggacagcggg cgcaggctac ccttacctgg gcctgggata cgcgtgacct
     1501 gcagccgggg agctacgagc tggagttcac gatcctgccg caggggaaag tctggcggca
     1561 gacgctggat ctgctgcccg ccggcgagcg tccccagccg gaagctgtct ggaaggaagc
     1621 gcagcttgac tgctgcgtgg tggattatat ttccgggacg gaggcagaac gcgacctgga
     1681 caggctgctg gaaatcatcg aggaacaaag ccggctggtg agcgagcaga tgggcgtaag
     1741 cctggaacag cccgttaaag taaccctggt accgagggtg ttgggacatg gcgggttcgc
     1801 cggggaggag atcacgatct cgtacctgga ccgattatat accgccagca atctggagat
     1861 gatcttccac cacgaactgg tgcacatctt cgattctcag ctggggggag acctgcgccc
     1921 aaccatgctg gtggaagggc tcgcagtcta tttgagtggg ggccatttta agccggagcc
     1981 catcctggaa agagcggctg tgctgctgcc agccgaaccg ggttgtattg tgtgcggtat
     2041 gggcgaatat gttccgctgg caaagctggt tgatgatttt tacagctctc aacacgaagc
     2101 cggatatctg caggcggcgg cgctggtgga attcctggtg gagagctatg gctgggaagg
     2161 cttcaatgcc ttctaccggg atattcatcc cgaccctgaa ggcagccagg cagagtccat
     2221 ggaggcttcc ctgcaggcgc attttaggct cagtctggca gagctggatg aacaattcca
     2281 atcccgcttg aggcaggtaa ggctcgatcc gaagtgggta gaagacgtaa agttgagtgt
     2341 cgagttctat gatgcggtgc ggcgctacca ggagcgtttt gatccgtcgg cgtacttccg
     2401 gactgcctgg ctgccggata cagcgcagat gcaggctgag gggatcaccg cagatttcat
     2461 acgccgcccg cagcaggccg tgaatatcat cctggaaaat ctattgacag cggtagggga
     2521 ggcgcttaaa gcggggcagt accaccaggg gctggagcac ctggaaacgg tgcagtacct
     2581 gttggatcat ctccccggcg aaatcaacac ggcacaggca aattaaacag aaaccggcag
     2641 gatttgcgcc tgccggcgat tggaaatctt gaagcctgat taagggcagg agtaaaagag
     2701 gcccgaaaga ctgtcgaagg gggagacgca gtacaggttg agggtatcaa aggctaacag
     2761 accaacaccc acgatacaca ccaggaccaa caggcagcca acaccaacaa cccacctggt
     2821 acggttggtc tgcttttctt cgtactcctc ttcatagacc ggctcgccag gaccgggcgg
     2881 cacctgacca gtgtagaccg gggcgggagg cgctggaggg gtataagccg gaggaggata
     2941 attgacaacc ggctgcgggg caggctgcgc ggctggtata gccggctcgg gaacaccgcg
     3001 cgggacagaa gcgacggtgg cattggggtc gaactgagaa aattcgtatg ccaggctgac
     3061 cgtctcgccc aggaagatca cttcgcccgg acggaggaga tggggaccca tcaaacgctg
     3121 cccattgacg aatgttccgt tggtcgagcc agtatcctcg atctggtaaa aaccgccctg
     3181 gatgtaaacg tgggcatgct tgcgcgagat ttcggcatcg ttgattacga tggtattgtt
     3241 gatatcccgc ccgatggtga tctccggttg gagcagatcg aaggtctgac cgggattggg
     3301 gccggtgcgc atgaccaggc gataggatga ttcagccatc tttcctcctc aatgttcccc
     3361 aaacgttggg gtcccctagc aaaggggtcc ccaggtaatt ggggtaaacg atccacattc
     3421 ggccctgatt gtccagccga aacacctata gtgtactgag atttcgagag catgtcaaca
     3481 aaaattataa atatcctcca tccgtcaata gacgttcagg gtatgaaata cgttccgggt
     3541 tcgtggaggt atctccaaca tctcgttaac atctttttga tcaaattcct atataagcag
     3601 ggtagactta atagacgtgg gaaaagagtt tctaccacaa gcaattattg gtagcggagt
     3661 gaaaaaatga atctggaaaa atatactcaa aaatcgcaag aagcgatcct ggctgcccag
     3721 cggatggcac aggactataa ccatccatcg atcgagccgg cgcacttact gctggcgctg
     3781 ctgcggcagg aggatggggt ggtgccggcg gtagtaacgc gagtggcggg gagcgtgctg
     3841 gcgctgcgg
//
LOCUS       DATMXK010000262         3802 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_150474, whole genome shotgun sequence.
ACCESSION   DATMXK010000262 DATMXK010000000
VERSION     DATMXK010000262.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3802)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3802)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3802
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150474"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            235..>3802
                     /locus_tag="VN363_04810"
     CDS             235..>3802
                     /locus_tag="VN363_04810"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family serine peptidase"
                     /protein_id="HWQ83863.1"
                     /translation="MKQRNPVQILFSILLLGGMILSMVQPAAVTAQTSGPQTPEISVD
                     PQVLEQMASDGAASYWIDLAGNTELASAEAMGWSERGWFVYEQLSKTAELSQASVVAY
                     LKESGIKFKSFWIKNTILVTSSDLAVLNGLMSFPEIEAIRARRTYQLHEPDTRNAVLD
                     NGVNAVEPNLTHINADDAWALGYTGLGITVANIDTGVRYTHDALVNQYRGNQGGGAFS
                     HDYNWFDPYGNFNVPTDGNGHGTHTMGTMVGDDGGENQIGIAPGADWMACRGCDTSSC
                     SDFALLTCAEFVAAPTDVNGNNPDPSLRPDAVNNSWGDCDMSYNGWYQTVVDNWQSAG
                     IYPIFSNGNASNCGYPTPPGLGTVGNPARYGNVTGVGSSGEQNGEYASHSNWGPSDVE
                     DTINAVPGFEFMKPQVLAPGVSIRSSTPGSDSEYQDGWTGTSMSAPHVTGLVALMWQA
                     APCLVGDYATTEVMIEDTAVAIVYEDGSPDTPGNDPNYATGWGEIDALAAVQAAAAFC
                     GDSTLDGTVTDAATSLPLAGAQVTITSANPDNNRTVFTNADGYYNAAVFADTYDIAAS
                     KFGYTPESVSGVVVTTGSTVTTDFALDELPPSLVSGFVYEGGIFGGAAHGYPLYSSLT
                     FSAPGFSQTVYTDPFTGAYEIELYLNQEYNVTVAAVPGGYAPFVGTFTPDTDPDTQDY
                     TLFVDVVSCAAPGYQPDYEIYWDFETSDGGFVQGGTTSFAWGEFTSGPGEGHSGTKGL
                     ATNPGGDHSDNEDGYIISPPIDLTGFGTDTPALQYWEWRETEFFFDFARVDVTKDGGA
                     TWTTVWGPESASEATQYTQKSITLDPTYNVVDFQFRFYFTSDGSVTYAGWYIDDIGIA
                     RIPLPPAVTVYSSDFETDNGGFTISGDDPSWEHGAPTSGPGAAHSGANVWATNLSGNY
                     NNNELSYITSPVIDLSAHTGLTPTISFWHWMDSAINIFDWGAVEATNDGGATWNTVWE
                     MFGNIPTWSFQSLALDPSYAVSNFQFRFHLQTDASGQYPGWYIDDVEIRVAEDVSLAV
                     PCVPVQGGVVAGTVFDGNTLAPLTGATVTSDTGIVTETFAIPEDPIEGFYWLFQPTET
                     DPEAIGFTASMEDYSPDSAVVSVAADAITQQDFTLSAGVLEFDPLALEATMTIGDPPA
                     SDIFTITNNGDGAVNFELRERNRGFVPLSIPAFRGTLPQSSAA"
BASE COUNT          806 a         1137 c         1044 g          815 t
ORIGIN      
        1 tccagctaat tccgcatcag atctactttc caacctgact tacctgtcct ccggaattgc
       61 ggaggatttt atgtggtaat tccccccagg ccacgaagga ggttttggtc ctattttcat
      121 tccctttctc tccaatttaa caacccacca ttcatcagcc ggagagcctt gatatcccaa
      181 gattgttcac cctatatatc gaacctgatt cacccatcat taaggagaat gcatatgaaa
      241 caacgtaatc cggttcaaat cttgttttcc atcctgctgc tcggcggcat gatcctgagc
      301 atggtccaac cggctgctgt cacagcccag acctcaggac cccagactcc ggaaatcagt
      361 gttgacccgc aggtgctgga gcagatggct tccgatggcg ctgccagcta ttggattgac
      421 cttgcgggga atactgagct ggcctccgct gaagcgatgg gctggtcgga gcgcggctgg
      481 tttgtctacg agcagctttc caaaaccgct gagctctccc aggccagtgt ggtcgcatac
      541 ctgaaagaat cgggtataaa gttcaaatct ttctggataa aaaacaccat tctggtcacc
      601 agctcggacc tggctgtctt gaatgggctg atgagcttcc cggaaattga agccatccgc
      661 gcccgccgga cataccagct tcacgagccg gataccagga atgcagttct ggataatggc
      721 gtcaacgctg tcgagcccaa cctgacccat attaacgctg acgacgcctg ggcgctgggt
      781 tacaccgggc tggggatcac cgtcgccaat atcgataccg gtgtaaggta cacccacgat
      841 gccctggtca accagtatcg cggcaatcaa ggtggtggtg ccttcagcca cgattacaac
      901 tggttcgatc cctacggaaa ctttaacgtt cccaccgacg gcaacgggca tggcactcat
      961 acaatgggca ccatggtcgg cgatgatggc ggtgagaatc agatcgggat cgccccgggc
     1021 gcggactgga tggcctgccg cggctgcgat accagcagct gttccgactt tgccttgctc
     1081 acctgcgcag aattcgttgc tgcacccacg gatgtgaatg gcaacaaccc cgacccgagt
     1141 ttgcgcccgg atgcggtcaa caattcctgg ggcgactgcg acatgagcta caatggctgg
     1201 taccagaccg tggttgataa ctggcagagc gcgggaatct acccgatttt ctccaacggg
     1261 aacgcctcca actgtggtta ccccaccccg cccggattgg gcacggtagg caacccggcc
     1321 cggtatggca atgtcaccgg agttggctcg tcgggtgagc agaatggcga gtatgccagc
     1381 cattcgaact ggggtccttc ggatgttgaa gataccatca atgcagtccc cggctttgag
     1441 ttcatgaagc cgcaggtcct ggcacccggg gtgagcatcc gctcctccac gcctgggagc
     1501 gattctgaat accaggatgg ttggactggc acatcgatgt ctgcgccgca cgtcaccggc
     1561 ctggtagcct tgatgtggca ggcagccccc tgcctggtgg gcgattacgc cacgaccgag
     1621 gtcatgatcg aagatacggc tgtggcgatc gtctatgagg atggctcacc agacactccc
     1681 ggtaatgatc caaactatgc aaccggctgg ggcgagatcg atgccctggc agccgtccag
     1741 gcagcagcag ccttttgcgg cgacagcacg ctggatggca cagtcaccga tgctgcgact
     1801 tccctcccac tcgccggagc acaggtcacg atcacgagtg ctaacccgga caataaccgc
     1861 acggttttca ccaatgccga tggatattac aacgcagcgg ttttcgctga cacctacgat
     1921 atcgcagcca gtaaatttgg ctacactccg gaaagtgtaa gcggggtcgt cgtgacgaca
     1981 ggttccacgg tcaccaccga ctttgccctg gatgagctgc cgccatccct cgtatccggc
     2041 tttgtgtatg agggcggcat ctttggcggt gctgctcacg gctacccctt gtattccagc
     2101 ctgaccttca gcgcacctgg cttcagccag acggtctaca cagatccatt cactggcgct
     2161 tacgagatcg agctatatct gaatcaggaa tacaacgtca ccgtcgcggc agtgccgggt
     2221 ggctacgcgc catttgttgg cacgttcacc cccgatactg acccggacac acaggactat
     2281 accctgtttg ttgacgttgt gtcctgtgcc gccccgggtt atcagccaga ttatgagatc
     2341 tactgggatt ttgaaacctc tgatggcggc ttcgtacaag gaggaaccac ttcgtttgcg
     2401 tggggtgaat tcaccagcgg acctggcgaa ggtcacagcg gtacgaaagg cctggcaacc
     2461 aatccagggg gagatcattc ggataatgaa gatggctata tcatctcacc tccgattgac
     2521 ctgaccggat ttgggacgga tacacccgcc ctccaatact gggagtggcg cgagactgaa
     2581 ttcttcttcg atttcgccag ggtcgacgta accaaagacg gtggcgcaac ctggacaacc
     2641 gtctggggac ctgagagcgc ctccgaagcc acccaataca ctcagaaatc catcaccctc
     2701 gacccgactt acaatgtcgt ggatttccag ttccgcttct actttaccag tgatggttcg
     2761 gtaacctatg caggctggta catcgacgat atcggcatcg cccgcatccc gctgccgccg
     2821 gcagttacgg tttactcgag cgatttcgag acagataatg gcggctttac gatcagcggc
     2881 gatgatccca gctgggagca cggcgcaccc accagcggcc ctggggcggc gcattctggc
     2941 gccaacgtct gggcgaccaa cctgagcggc aattacaata acaacgagct cagttacatc
     3001 acctcacccg tgatcgatct gagtgctcac acaggcctga ctccgacgat ctccttctgg
     3061 cactggatgg attcggcgat caatatcttt gactggggag ccgtggaggc cactaacgat
     3121 ggcggggcta cctggaatac cgtctgggaa atgttcggca atatccccac ctggagcttc
     3181 cagagcctgg ccctcgaccc cagttatgca gtcagcaatt tccagttccg cttccatctg
     3241 cagaccgacg cctccgggca gtatcccggc tggtatatcg atgatgttga aatccgcgtt
     3301 gcagaggatg tgagcctggc ggtaccctgc gtaccggtgc agggtggggt ggtggcagga
     3361 acggtctttg acggcaacac cctggccccg cttaccggtg ccactgtcac ctcggatacg
     3421 ggcatcgtta cagagacctt tgcgatccct gaggatccca ttgaaggctt ctactggctc
     3481 ttccagccga ccgaaaccga tccggaggca atcgggttta ccgcttcgat ggaggattac
     3541 tcccccgatt cagcggtcgt ctcggttgct gccgatgcga tcacccagca agacttcacg
     3601 ctttcagccg gggtgctgga gtttgatccg ctggcgctgg aagccaccat gacgattggc
     3661 gaccccccgg ccagcgacat ctttacgatc acaaataacg gcgacggcgc agtaaacttc
     3721 gagctgcgcg agcgcaatcg tggctttgtc ccgctctcca tcccggcctt ccgggggacg
     3781 ctgccacaga gcagtgcagc cc
//
LOCUS       DATMXK010000263         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51558, whole genome shotgun sequence.
ACCESSION   DATMXK010000263 DATMXK010000000
VERSION     DATMXK010000263.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51558"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..502
                     /locus_tag="VN363_04815"
     CDS             <1..502
                     /locus_tag="VN363_04815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ83864.1"
                     /translation="WWAANYASRYPQRVASLALLEPVFVFTGLKPALLLKLIPATLPF
                     APKSYRDAVLKEIGGSQEAELDLEDPVVRMIALGSEAFVRHIPMPVQITPEQMQAWEM
                     PVYAAMAANSALHDSEQAAAAARANVRQVQVKNWPGASHSLPMEFPAEIDAELLDFMQ
                     ASEASR"
     gene            complement(1327..2253)
                     /locus_tag="VN363_04820"
     CDS             complement(1327..2253)
                     /locus_tag="VN363_04820"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWQ83865.1"
                     /translation="MSANSAASGSTLTDSPGRMIDLHAMVFMVALAASLAAEGQLNNA
                     KLLRAVIDSQLTRAARSLNLPSDRPALLDATGQALAFLAELDLPPELRSALMHSQVTL
                     AEGRLTAYRDIPDPYVCRTCGSLVLQLPASCAGCGAHPDTFKRIRSTYWIDAFDPFEA
                     LERLRSTPALLASALESARLDSALHPSSPQAEPAEGWTLSQAFSHLNDSQAVLDYRIN
                     LILEQDNPVLEAKALYEQAASASATAGELFAAYRASRARTIARLESLPLKDWWRRGRH
                     QEFGELTLTQQVSYFACHELDHLPQLLRLAGM"
     gene            complement(2605..3003)
                     /locus_tag="VN363_04825"
     CDS             complement(2605..3003)
                     /locus_tag="VN363_04825"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWQ83866.1"
                     /translation="MSETVICPYCSSVIPADAEVCPMCGADVTPDELEDAPELTGESV
                     TTSGAVVIDAPLFSASPEIPQAPDLVTPLRTNPSPAPLQGEVFSGNQGLSGAPNNRPR
                     NVLILAGIVFLLVSCLCVIAIVATLAAQMR"
BASE COUNT          613 a          849 c         1069 g          569 t
ORIGIN      
        1 gtggtgggcg gccaattatg cctcgcgata tccacagcgg gtggcttcgc tggcgctgct
       61 ggagccggtg tttgtcttta ccgggttgaa acccgccctt ttgctgaagc tgatcccggc
      121 cacactgccc ttcgcgccga aaagttatcg cgatgcggtg ctcaaagaaa tcggcggctc
      181 ccaggaagcg gagctggacc tggaggatcc cgtcgtacgc atgatcgccc tcggctctga
      241 agccttcgtt cggcacatcc ccatgcccgt ccagatcacg ccggaacaga tgcaggcctg
      301 ggagatgcct gtgtatgccg ccatggcagc caacagcgcc ctgcacgatt ctgaacaggc
      361 cgccgccgca gcccgcgcca atgtccggca ggtgcaggtg aaaaattggc ctggcgcctc
      421 gcattcgctg ccgatggagt ttccggccga gatcgatgcg gagctgctgg actttatgca
      481 ggccagcgaa gccagccgct gagatccggg ggggcgcagc ggggctgatc tggaataccg
      541 ggttgcggac ccggcagggg cgcagcggac ctggacaggc agtgtgtccg tcgtgttccg
      601 ctgcgcccct acggtttgga gtgggtgcag gggtgcttcg ccggattgct tcactgcgtt
      661 cgcaatgaca tcgcgaacag gtgtcattgc gagacgcact ttgtcgaagc aatctggtct
      721 ttacctggag tgagctctgg cgcgcaaaca ccagattgct tccctggatg agtaatgacg
      781 gcaaaataca atccacatag aagggggagc agaaaccgag ttttcaagaa aaaactcggt
      841 tttcgcgaaa aacggaggcg cttcgacttc acgcggagca ttccgctctg cgcgcttatc
      901 ttgctcaatg cgatcggata ttcagcactg tggcgatgac aaacagcacc gcgtaaaccg
      961 cccacagcct cctgtccagg cgggcgaggg gccaccagta gaacatgact gtggcaaggt
     1021 acagggtgcc atatggaagc aggatcagca gaataccggc tgcgcggcag cagctcgggg
     1081 ggaacgctgc gcagggctcc catctcggcg tggcgggtaa gcgccaggac cggctttcag
     1141 atggtattca tgccctgggc gatgatccgg tcgtcgagaa caaccaccaa gtgtgcaaat
     1201 tgtgaaccaa tccgtgaaat ccgttccgga aatccgttgt agactcctga cggcgctccg
     1261 ggtctgcttg ctgcaaccgg cggacggttg gctgtgggcg ggtggcaaag cggactgaag
     1321 tccgcgctac atgccggcca ggcgcaggag ctggggcagg tggtcgagct cgtggcaggc
     1381 gaagtagctg acctgctggg tgagggtgag ctcgccaaat tcctggtgcc ggccgcgccg
     1441 ccaccagtct ttgaggggca ggctttccag gcgggcaatt gtgcgcgccc gcgaggcgcg
     1501 gtaggcggcg aacagctcgc cggcggtggc ggaagccgac gctgcctgct catagagggc
     1561 tttggcttcc aggaccgggt tgtcctgttc aaggatcagg ttgatgcggt aatcgagcac
     1621 ggcctgcgag tcgttcaggt gggagaaagc ctggctcagg gtccagcctt cggcgggctc
     1681 ggcctgcggc gaagatgggt gcagagcgga atccagcctg gcggattcca gagcactggc
     1741 gagcagggcg ggggtgctgc gcaggcgctc gagggcttcg aagggatcga aggcatcgat
     1801 ccaataggtg gagcggatgc gtttgaaggt gtccgggtgg gcgccgcagc ctgcgcagct
     1861 ggccggcagc tgcaggacca ggctgccgca ggtccggcag acatacgggt cggggatatc
     1921 ccggtaagcg gtcaggcggc cttcggccag ggttacctgg gagtgcatca acgccgaacg
     1981 cagctcggga ggcaggtcca gctctgccag gaaggcgagc gcctgcccgg tcgcatccag
     2041 gagcgccgga cggtcactgg gcaggttcag gctccgggcg gcgcgggtca gctgcgagtc
     2101 gatcacggcc cgcagcagct tggcgttatt gagctggcct tcggctgcga ggctggcagc
     2161 gagcgcgacc ataaagacca tggcgtgcag gtcgatcatc ctgccggggg aatcggtaag
     2221 cgtgctaccg gaagcagcag aatttgcgga catggctcct ccagaaatac ccggataaat
     2281 actcaggcaa gcagtataag atattgtata tccgaattat ctgaagggca agctgccgcg
     2341 gggaagtgga ggagttaatt tcccattccc ccgggagaca aagctggatt tttgagcgcg
     2401 ggggagagtc gggtacggtc aggtcgtgcg tcgccaggaa acattggctt tgccaggacg
     2461 cctgagcgca gccatgacgc cttagcgcag ccaggactcc tggatttgtc caggacgcct
     2521 ggacgtcgcc aggaaacctt ggcgcagcca ggacgcctgg atttgtccag gactcctggt
     2581 actgccagga cgcctggggt tgatctagcg catctgggcg gccagggtgg cgacgatggc
     2641 gatgacgcac aggcagctga cgagcaggaa cacgatcccg gccaggatca gcacattgcg
     2701 ggggcgatta tttggggccc cggataggcc ctggttcccg gagaagacct ccccctgaag
     2761 tggggcaggt gagggattcg tgcgcagggg tgtgaccaga tcgggcgcct gggggatctc
     2821 gggcgaggcg gagaataagg gggcatcgat taccaccgct ccgctggtgg tcacggattc
     2881 acccgtgagc tcaggggcat cttcgagctc atccggggtt acatccgccc cgcacatggg
     2941 gcagacttca gcatcggcag ggataaccga tgaacagtaa ggacagatga ccgtttcaga
     3001 catggcaggt tcctccttat tggatgctgg attcagagat attgtaacgg cagaacgcta
     3061 attttgaaaa aagattgcct ggattggatg tgaatttaat
//
LOCUS       DATMXK010000264         2166 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_68297, whole genome shotgun sequence.
ACCESSION   DATMXK010000264 DATMXK010000000
VERSION     DATMXK010000264.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2166)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2166)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2166
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_68297"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(115..528)
                     /locus_tag="VN363_04830"
     CDS             complement(115..528)
                     /locus_tag="VN363_04830"
                     /inference="COORDINATES: protein motif:HMM:NF014608.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="OsmC family protein"
                     /protein_id="HWQ83867.1"
                     /translation="MGTVNLQWVRQNMFVANDSRGYSLNISSSENEAGGRMGAKASDL
                     LLVSAAACSSWDIAEIMAKQRQPMRDLQVRCIGEQMSDPPFTFTKIHIVYTVYGPVDP
                     DKLEQAIRLSEDKYCSVISTLRLGIPVTSEFEICP"
     gene            complement(556..1572)
                     /locus_tag="VN363_04835"
     CDS             complement(556..1572)
                     /locus_tag="VN363_04835"
                     /inference="COORDINATES: protein motif:HMM:NF015338.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endonuclease/exonuclease/phosphatase family
                     protein"
                     /protein_id="HWQ83868.1"
                     /translation="MNATGPAVRELANFVLTAGVAGLGLLTILGWLASSWWLWDVASH
                     FRLQYGILLAVGAVAAWWLGNPVLGLIAAMLAFSNLVVLLPLYIRPRNVLAATHPALT
                     RRVLFANLWQPNQEYASLRALVEKTSPDLVLLVESNQRWLDELEPLRPAYPYRVVQPR
                     EDNYGLAVLSRWPVLDSEIHTLSRAGVPTILARVDMAGQLVSVIVTHPPPPKSLAETR
                     ARDEQMSSLAPLAADLRPLLVCGDLNLTPWSPVFWRFLRQSGLADSARGFGIQPSWPV
                     GKPYMGVPLDHCLVSPEIIVLDRRLGPAFGSDHYPVIIDFSVVQSKRTFADGGMDDSS
                     ISAS"
     gene            complement(1607..>2166)
                     /locus_tag="VN363_04840"
     CDS             complement(1607..>2166)
                     /locus_tag="VN363_04840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83869.1"
                     /translation="MLQRSQDFVAATQSSLLACHIQWLAVEKGEPGATMEDLRYYLAS
                     YASVIAGKLSQVNRDYAAARPYYLAFFALVQEEDPLWSRMRGLINPMLAYFWVNAGRE
                     LELNTSTWNLNGASPAQVAVYISNHHNTELRELWREITTELARVNPTLLRRIANQIDL
                     SRAEGPENARTAQQLYGILSSVNAG"
BASE COUNT          482 a          615 c          645 g          424 t
ORIGIN      
        1 gtgggcgagg tatgggattc gggtgccttt gcgcacctgt cggcggtgca gctcggaggc
       61 gtacagcagc gcctgatcaa aacgtgagct gagtttgggt cgcatggcaa ttcctcaggg
      121 gcagatctcg aattcgctgg tgaccgggat gccgaggcgc agggtgctga ttaccgaaca
      181 gtatttgtcc tccgagaggc ggatggcttg ctccagttta tcggggtcca ccggaccata
      241 gacagtataa acaatatgga ttttcgtgaa ggtgaagggt ggatcgctca tctgttctcc
      301 tatgcagcgt acctgtaaat cgcgcatggg ctggcgctgc ttggccataa tttcggctat
      361 atcccagctg gaacaggctg cagcggaaac caacagcaaa tcagacgcct tcgcccccat
      421 cctgcccccc gcttcattct ccgatgagct gatattcagg ctgtagcctc gagaatcgtt
      481 ggcaacaaac atattttgtc tcacccattg tagattaacc gtgcccatat taccgctcct
      541 tagaaaattt gattgtcatg aagccgagat ggagctgtca tccattcctc catcggcaaa
      601 ggttcgcttg gattgaacaa cgctgaaatc gatgatcacg ggataatgat ccgagccaaa
      661 tgccggcccc aggcggcgat ccagaacgat gatctctggc gagaccagac agtggtccag
      721 cggtacaccc atgtatggtt tgccaaccgg ccagcttggc tggatgccga agccgcgggc
      781 gctgtcggcc aggccgctct ggcgcagaaa acgccagaaa accggtgacc agggtgttag
      841 attcaggtcc ccacatacca acaaggggcg gagatctgcc gccaggggag cgaggctgct
      901 catttgttca tccctggccc tggtctcggc caggcttttg ggcggcggag ggtgggtgac
      961 gatcacgctc accagctgcc cagccatgtc cacgcgggcc aggatggtgg gaacaccggc
     1021 gcggctcaat gtatggatct cggaatccag caccggccag cggcttaaca cagccaggcc
     1081 gtaattatct tcgcgcggct ggaccacccg gtaaggatac gccgggcgga gcggctccaa
     1141 ctcatcgagc cagcgctggt tactttcgac cagcaaaacc aggtctggag aggtcttttc
     1201 caccagggca cgcaggctgg catattcctg gttgggctgc cagagattag caaaaagcac
     1261 gcgccgggtc aaagccgggt gtgtggctgc cagaacattc cgcgggcgga tatacagagg
     1321 taataatacg accagattgg aaaacgccag catggcagcg atcaatccaa ggaccggatt
     1381 ccccagccac caggctgcca ccgcccccac agccaggagg atcccatact gcaatcggaa
     1441 gtgactggca acatcccaca gccaccagga actcgccagc caacccagga ttgtaagcaa
     1501 gccgagcccg gcaacaccgg cagtaaggac aaaattcgcg agttcccgga ctgccgggcc
     1561 ggtggcgttc atgcagctct tcagcctgtc gggcgagagc ctattttcag cccgcgttta
     1621 cgctggaaag gatcccatac agctgttggg cagtgcgggc gttctctggt ccttcagcgc
     1681 ggctaagatc aatctgattg gcgatgcggc gaagcagggt ggggttgacc cgcgccagct
     1741 cggttgtgat ctcgcgccac agctcgcgca gctcggtatt atggtggttg gagatataga
     1801 ctgcaacctg ggcgggtgag gctccgttca gattccaggt ggaggtattc agctccagct
     1861 cacgaccggc gttgacccag aaatatgcca gcatgggatt gatcaaacct cgcatccggc
     1921 tccagagcgg atcttcttcc tgcaccaggg caaagaaagc caggtaatag ggacgggcag
     1981 ctgcataatc ccggttcacc tgagagagct tgccggcgat gacagaagca tatgaggcca
     2041 ggtaatagcg caaatcttcc atcgtggcgc ccggctcacc cttttccacc gccagccact
     2101 ggatatggca ggctaacagg ctggattggg tggcagcgac aaaatcctgc gagcgctgca
     2161 gcatcg
//
LOCUS       DATMXK010000265         3039 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_15369, whole genome shotgun sequence.
ACCESSION   DATMXK010000265 DATMXK010000000
VERSION     DATMXK010000265.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3039)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3039)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3039
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15369"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1292
                     /locus_tag="VN363_04845"
     CDS             <1..1292
                     /locus_tag="VN363_04845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83870.1"
                     /translation="KNPELGSFEVIQVQFEQGEQHEFAAGLPEHKLNRPLEAQVEPSD
                     MDTQSILDAYREDLDTALEEELASNPDFVRIAGRWFPRALLVDVNLGHLNLAEAVLDM
                     SGGGPISTTDLIEQIELKADVNPKLIEFSLDLALQEDPRFDEVGPAGQVLWYLQRLEP
                     QEVLEPPVYLRYPGIEYDRSLLTASMVALEKSLGDELSPTGYSQPGQDEVLVNLIYPH
                     WRSGSLPLSSRLRHLFPTAYEAPRIRFVLVDGDSGEKFPGWVVPEKRYVSGLRDWYAK
                     QGLVPGSQVRVRRGDQPGEVIIDTSSRRSSREWVRTVLVGSDGGVVFAMLKQVINAVF
                     DERMAIVAPDTDALDQVWTRMSKDRQPFEKTVVNIVRELAKLNPQSHVHASELYAAVN
                     VIRRCPPGPILAQLASRPWFVHVGDLHYRFNDSEYAG"
     gene            1357..1791
                     /locus_tag="VN363_04850"
     CDS             1357..1791
                     /locus_tag="VN363_04850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83871.1"
                     /translation="MIEPRRISLVKPTLQTHFHIDFDWWRQVDRDWRVYLRSYLCPEH
                     QEAFADTGIEEMVDWVDPETAEVQQVDGIQHVLISHCARQESFISEQTTLVDSVFRMF
                     LANGNTPLTPVEMAGALNRQPEMILRTLTGPRVYKGLRPCLD"
     gene            1800..1872
                     /locus_tag="VN363_04855"
     tRNA            1800..1872
                     /locus_tag="VN363_04855"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:1833..1835,aa:Arg,seq:tct)
     gene            1938..2363
                     /locus_tag="VN363_04860"
     CDS             1938..2363
                     /locus_tag="VN363_04860"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWQ83872.1"
                     /translation="MIVISLKSNDPSTPRQWAHTSDAFGEARSGFWRINVHSPWRPPT
                     DVFETEDVLIVRMEIAGMRESDFAIELNGRLLSIRGVRPDANERRSYYQMEIRFGEFS
                     IEIELPFPIYADRVEASYRDGFLRLVLPKANPRQIHIED"
     gene            2383..>3039
                     /locus_tag="VN363_04865"
     CDS             2383..>3039
                     /locus_tag="VN363_04865"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWQ83873.1"
                     /translation="MPASRWNSNLLEMLNWLKEEEASMEEFLLRPIPARIRTDEHTEG
                     DSASEAQNTINNIPPVLPILPLRGLVVYPETAVPLTIGQPRSIRLVDDVMGNDDRMIG
                     LVASRDPDLETPEPADLYTVGTVAIVHRLFRAPDGSVRLVVQGITRFRLGEFIQTEPY
                     LKAQIELIPETLEDGVELEALARNARDQFEHIADMIPSIPRELVASVLALEDPLETVY
                     T"
BASE COUNT          707 a          737 c          839 g          756 t
ORIGIN      
        1 gtaaaaatcc cgagctaggc agttttgaag taatccaggt tcaatttgaa caaggtgaac
       61 agcacgaatt tgcagctgga ctaccagagc ataaactcaa ccgcccgctt gaagcgcaag
      121 ttgagccatc agatatggat actcagtcga tccttgacgc gtatcgtgaa gatctcgaca
      181 ccgctcttga agaggaactg gcttctaacc cggattttgt acgtatcgct gggcgctggt
      241 tcccacgggc actgttggtg gatgtcaatc tggggcatct gaatctggca gaggcagtcc
      301 tggatatgtc gggcggtgga cctatatcga cgaccgattt aatcgagcag atcgagctta
      361 aagcggatgt aaaccctaag ctgatcgaat tttcattgga tctggccctt caggaagatc
      421 cgcggtttga tgaggtcggc ccggcaggtc aggtattgtg gtacctccaa cgtttggaac
      481 cccaagaggt gctggaacca cccgtgtatc tccgctatcc gggcattgaa tatgatcgtt
      541 ctttgctcac ggcgagtatg gttgcattgg aaaaatcgtt aggggatgag ctgtcaccaa
      601 ctggttattc tcagccaggt caggatgaag tgctggtcaa tctgatttat ccccactggc
      661 gttcgggatc acttccctta tccagccgct tgcgtcattt attccctact gcgtatgaag
      721 cgccccggat tcgattcgtg ctggtggacg gagatagcgg tgagaaattt cccgggtggg
      781 tggtacccga gaagcggtac gtatctggtt tgcgcgattg gtatgccaaa caaggtctgg
      841 ttcctggcag ccaggtgcga gtacggcggg gagaccaacc gggtgaggtg attattgata
      901 cgagctcacg gcgctccagc cgtgaatggg tccggacagt gctggtaggg tcagatggcg
      961 gagtggtttt tgccatgttg aaacaggtga ttaatgccgt ttttgatgaa cgaatggcga
     1021 tcgtcgcccc agataccgac gcccttgatc aagtctggac gcggatgtcg aaagatcgcc
     1081 aaccatttga aaaaacggtt gttaatatcg tgcgggagct ggcgaagttg aatcctcaaa
     1141 gtcatgtgca tgccagtgaa ttgtatgctg ctgtaaatgt aatccggcgc tgcccgcctg
     1201 gcccaatctt agctcaactg gcttcgcgac cctggttcgt gcatgttggc gatttacatt
     1261 accggttcaa tgattcggag tacgccggat gatctttccc tgtttactgc catctattca
     1321 gcagggtaag taccctacga cacggaggtg gcataagtga ttgaaccaag gcgcatcagt
     1381 ctggttaagc ctaccctgca aacccacttc cacatagact tcgactggtg gagacaggtc
     1441 gatcgagact ggcgggttta cctgcggagt tatctttgtc cggaacacca ggaggcgttt
     1501 gcagatactg gaatagaaga aatggtagat tgggttgacc cggaaactgc tgaagtacag
     1561 caggtggatg gcatccagca tgtgctgatt tcacactgcg ctcgccagga atcttttatc
     1621 tcagagcaaa ccactctggt tgattcggtt ttccggatgt tcctggcaaa cggaaatact
     1681 ccattgaccc ctgttgagat ggctggggcg ctcaaccggc agccagaaat gattttgcgg
     1741 actcttactg ggccacgtgt gtataaggga ttgagaccct gcttggattg aatatttttg
     1801 cccccgtagc tcaatggaca gagcaagtgc cttctaagca cgaggttgtg ggttcgaatc
     1861 ccgccggggg cgctcacgaa ctctccctgt tatggtatga aacctggctg tggcaattaa
     1921 ttgacaggag gtaatcgatg attgttatct ctttaaaatc aaatgatcct tctactccac
     1981 gccaatgggc gcatacttca gatgcttttg gcgaggcaag atcgggtttc tggcggatca
     2041 atgtgcattc tccctggcgc cctcccacag atgtattcga gaccgaagat gtattaattg
     2101 tccgcatgga aattgccggg atgcgagaat ccgactttgc gattgaatta aacggaaggc
     2161 tgctttcaat ccgaggtgtg cgacctgacg ccaatgaacg ccgctcttat tatcaaatgg
     2221 aaatccggtt cggtgagttc agtatcgaga tagaactgcc cttcccgatt tatgctgacc
     2281 gggtcgaagc cagctaccgc gatggattct tgcgcctggt gttgcctaaa gcaaacccca
     2341 ggcagatcca tatcgaagat tgagccatag attggatagc taatgcctgc ttcacgatgg
     2401 aattcaaacc tgctagaaat gctgaactgg ctcaaggaag aagaagcctc catggaggaa
     2461 tttttgttac gccccatccc ggcccgaatc cgtaccgatg agcataccga gggggatagt
     2521 gcaagcgagg cacagaacac catcaacaat atcccacctg tgctccctat cttaccgttg
     2581 cggggtctgg tcgtctatcc agagacggca gttccattga caattggaca accacggtca
     2641 atccgattgg tggatgatgt gatgggaaat gacgaccgca tgattggcct ggttgcatcc
     2701 cgtgatccag atttggaaac acccgagccg gctgatctgt atacagtggg aaccgtagca
     2761 attgttcatc gactattccg tgccccagat ggatccgtcc ggctggtggt ccagggtatt
     2821 actcgtttcc ggttaggtga atttatccag actgagccat atctgaaagc ccagatcgag
     2881 ctgatcccgg agaccctgga agatggcgtt gagcttgagg cgctggcccg caacgcccgt
     2941 gaccagtttg agcatatcgc ggatatgatc ccttctatcc cacgcgagct ggtcgcctcg
     3001 gtcctggctt tggaagaccc gctggagaca gtttatacc
//
LOCUS       DATMXK010000266         5063 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_80377, whole genome shotgun sequence.
ACCESSION   DATMXK010000266 DATMXK010000000
VERSION     DATMXK010000266.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5063)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5063)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5063
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80377"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1909)
                     /locus_tag="VN363_04870"
     CDS             complement(<1..1909)
                     /locus_tag="VN363_04870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83874.1"
                     /translation="MTLSETGLWLLPLVLVASLAALFLVTRRWRKATNDRLSQLRTQL
                     RQLETTHKGLAQLAERYPANSREPYSKPAQWVHSRLEAVQAEVVDFKNGYIDLQEKLH
                     ELNENRFRALVAAPYLWNDLYLSASRLDQQTSAIISHQQEIQTWLDQLQGLAWEVAVQ
                     ARKASQLQKQIDAGMDALLNEHMYGAALEAAQVEADQWLSALAQIPAYFLSGDESAVL
                     QQAEPAGAAEVFSLAVRAQPALENLDRQVEDWQNQHQDAVRQVSQLQSLLGGLQRLFT
                     EMPATLELASLRSQYDRMEQIAQTLAATLERLELESTPTLIEETQRQQQACAFLEREL
                     RQARQQLGSLNKTLPELDAAINLVSEQIEQLAAGPVHPLRWGATRSLLEEQYQKALAL
                     HQHQPPHSPDRVRQDLEAATQLLVEVKLLDQQTQVTAEGHTELLRLLESPELASDQRW
                     LEGSRALTEKIAAYDPVNWPRLDGTTHLPEELHEFTTRLQQLTAQGMKKPLPESELPA
                     RLDEARHLARSSAELRSRIIKIQTRLDDIQAMEQDSRQQVDGAARILAQVTALERSND
                     ALARVVHADLARLEPALKQAQTELDQRYRGSIEKKVRLTESLTSRLDQSLRHWQVHFN
                     KEIEAAKLELSE"
     gene            complement(1906..3777)
                     /gene="htpG"
                     /locus_tag="VN363_04875"
     CDS             complement(1906..3777)
                     /gene="htpG"
                     /locus_tag="VN363_04875"
                     /inference="COORDINATES: protein motif:HMM:NF003555.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone HtpG"
                     /protein_id="HWQ83875.1"
                     /translation="MDKPQTTPADPAGIPFKAETRQLLDILIHSLYTEREIFLRELLS
                     NAADALTRLDFELLTNRDVADPDLELAIRVFPDKENKTLTIQDTGIGMTATELAENLG
                     TIAHSGARKFMEAVQESGKAAANLIGQFGVGFYSAFMVAESIQVTSRSYLPVADAAAW
                     TSTGSDTYTITPAEKAERGSTVTVHLKEDALEFADESRLREIIRKHSDFIPFPIYIGD
                     GTEQVNKRSALWRQSPREVKEEEYTEFYKQLTLDFEAPLTSAHMVVDAPVQMYALLFI
                     PSSPERSMFSLRKEDGLKVYARKVLIQEYNKTLLPEYLGFVQGVVDSEDLPLNVSRES
                     VQSNKVMAQLKKLVTSKVLDTLKKLAKDDPEKYAQFWKAYSRFIKQGIAIEPAEPTGL
                     YPLLRFRTLHQPEELVSLDEYITHMQAGQEDIYYILGDDERSVVHSPHLDVIQHYNYD
                     VLILTDPVDPFMLVRLHEYQGHKLVNVANADLTLPAVEAEKDAQETSRLPADASASLV
                     ERFKRQLGERVTDVRITGRLSGSPARLVDPEGALNQEMQRVYRLLDKEFDAPKKVLEL
                     NPAHPILVGLHEIGEEDELSKLLIEQVYEDALLIEGIHPDPASMITRIQEIMQRALK"
     gene            complement(3910..>5063)
                     /locus_tag="VN363_04880"
     CDS             complement(3910..>5063)
                     /locus_tag="VN363_04880"
                     /inference="COORDINATES: protein motif:HMM:NF020318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="DUF1786 domain-containing protein"
                     /protein_id="HWQ83876.1"
                     /translation="LTVDVGTGTQDVFLYDSRLDLENGFKLVVPSPTMIVHRRLRQAT
                     RQRQAVLLEGVTMGGGPSAWAAEEHARQGLPIYATPDAARSFNDDLEVVSQSGIEVVS
                     EDEASKLPDSVLRLQLRDFDFTALAIAFEQFGVSLKELAAIAVAVFDHGAAPPDVSDR
                     QFRFDYLDERLRAENRLSAFAFRAAEVPPIMTRLQAVVQSARQEAAQALGEIPLVVMD
                     TAPAAVLGATYDPLVAAAPRKIIANVGNFHTLAFRLGPTGIEGVFEHHTGLLDLPQLE
                     ALLTTLADGSLTHADVFGHHGHGALIYEPQPIDLSTGPFGIAVTGPRRSLMRDSKLRP
                     HFAVPFGDMMIAGCFGLLAAVADVLPELGEPIRASLNGQTGHTAPWDAE"
BASE COUNT          982 a         1370 c         1535 g         1176 t
ORIGIN      
        1 gctcagacag ctccaatttc gctgcctcaa tctctttgtt aaaatgcacc tgccaatggc
       61 gcaggctttg atccaggcgg ctggtcaacg attcagtaag gcggactttc ttttcaatgc
      121 tgccgcggta gcgctgatcc agttcagtct gagcctgttt caaagccggt tccaggcgag
      181 ccaggtcagc gtgaacaacc ctggctaaag catcattact gcgttccagg gcggtcacct
      241 gggcaagaat gcgggcagcc ccgtctacct gttggcggct gtcctgctcc atggcttgaa
      301 tgtcatccag acgggtttga attttaatga tccgggagcg cagctcagcg ctgctgcggg
      361 caaggtggcg cgcctcatcc aggcgagcgg gaagctcgct ctctggaagg ggctttttca
      421 tgccctgggc ggtcaattgc tgcaatcggg tggtgaattc atgcaattct tccggtaaat
      481 gggtagttcc atccaggcgc ggccagttaa ccggatcata tgccgcgatt ttttcggtaa
      541 gcgccctgga accctccagc cagcgctggt ctgaagccaa ttcgggtgac tcgagcagcc
      601 tgagcagttc agtgtgtccc tcggcggtta cctgcgtttg ctggtccagc agcttgacct
      661 ctaccaggag ctgggtggca gcctcaagat cctgccgtac ccgatctggg gaatggggtg
      721 gttggtgctg gtggagggca agggctttct ggtactgttc ttcgagcaac gagcgggttg
      781 cgccccatct taaggggtgc acagggccgg ctgccagctg ctcgatttgt tcgctgacaa
      841 gatttatcgc tgcatcaagc tcgggtaagg ttttgttgag gctgccgagc tgctggcgcg
      901 cctggcgcag ctcgcgttcc aggaatgcgc aggcttgttg ctgacgctgg gtttcttcga
      961 ttagagtggg tgtactttct agctccaggc gctccagggt ggctgccaga gtttgggcaa
     1021 tttgctccat ccggtcatat tggctgcgca gggaggctaa ctcaagcgtt gctggcatct
     1081 cggtaaacag gcgttgaagg ccaccgagca gactctgcag ctggctgacc tggcggactg
     1141 catcctggtg ttgattttgc caatcttcga cctggcggtc gaggttttcc agggctggtt
     1201 gggcacgcac cgccaggctg aatacttcgg cagcgcctgc gggttcggct tgctgcaaga
     1261 cagcagattc atcgccggat aagaaatatg ccgggatctg agcgagagct gacagccatt
     1321 gatcagcttc tacctgagcg gcttccagag ctgcgccata catatgctca tttagcagtg
     1381 cgtccatacc ggcatcgatc tgcttttgca gctggctggc tttacgggcc tgtacggcaa
     1441 cttcccatgc caatccttga agctgatcca gccaggtctg gatttcctgc tggtggctga
     1501 tgatggcact ggtttgctgg tccaggcggc tggcgctgag atagagatcg ttccacaaat
     1561 agggcgcggc caccagcgcc cgaaagcgat tctcgttcag ttcgtgcagc ttttcctgca
     1621 gatcgatata cccattttta aagtccacaa cttcggcttg cacagcctcc aaccgggagt
     1681 ggacccattg ggcaggcttg ctatacggct cccggctgtt tgccggatag cgctcggcca
     1741 gttgggccaa tcccttatgg gtagtttcga gctggcgcag ctgtgtgcgc agctgagaca
     1801 gccggtcatt cgtcgccttt cgccagcggc gagtgaccag gaataaggcg gccaggctgg
     1861 cgaccagaac taagggtaac agccacaggc cagtttcgga taaggtcatt taagcgctcg
     1921 ctgcatgatt tcctggatgc gggtgatcat actggctgga tcggggtgta ttccttcgat
     1981 taacagggca tcttcataga cttgctcgat caacagcttg ctgagctcgt cctcttcacc
     2041 gatctcgtgc aggccaacca aaattgggtg ggcgggattg agctcaagca cttttttcgg
     2101 ggcatcgaat tctttgtcca gcaggcggta gacgcgctgc atctcctgat tgagggcgcc
     2161 ttcgggatcc acgagccgag caggtgagcc ggataggcgt ccggttatgc gcacgtctgt
     2221 gactcgctca ccaagctggc gcttaaagcg ctccacgagg ctggcgctgg cgtcggcggg
     2281 tagacggctg gtctcttgcg catctttttc ggcttccacg gccggcaacg tcaggtcagc
     2341 gttagcaacg ttgaccagtt tatgtccctg atattcatgc aggcgcacca gcataaaggg
     2401 atcgaccggg tcggtcagga tcaggacatc atagttgtaa tgttggataa catccaggtg
     2461 agggctgtga accaccgaac gttcatcatc gcccaggata tagtaaatgt cttcctgtcc
     2521 cgcctgcatg tgagtgatgt actcgtccag agagaccaat tcttcgggct ggtgcaaagt
     2581 gcggaagcgc agcagtggat acagcccggt tggctccgct ggttcgatgg cgataccctg
     2641 tttaataaag cggctgtaag ctttccagaa ttgggcatat ttctcggggt cgtcttttgc
     2701 cagctttttc agcgtatcca acactttcga ggtcaccagt ttctttagct gcgccatcac
     2761 cttgttagac tgcacagatt cgcgggatac attcagaggt agatcttcgg aatcgaccac
     2821 gccttgcaca aagcctagat actctggcag caacgttttg ttgtattcct ggataagcac
     2881 tttgcgggca taaaccttaa ggccatcctc ttttcttaat gaaaacatgc tgcgttccgg
     2941 gctggatggg atgaataaca gggcgtacat ctgtaccggt gcatccacga ccatgtgagc
     3001 agaagtcagt ggggcttcaa aatccagggt gagctgcttg taaaactcgg tatactcctc
     3061 ttccttgact tcccgcggtg actggcgcca cagggcgctg cgtttgttaa cctgttctgt
     3121 tccatcgcca atgtaaattg gaaaagggat aaagtcggag tgcttgcgaa tgatctcgcg
     3181 caggcggctc tcatcggcga actccaatgc atcttctttg agatggactg tgacggtact
     3241 gccgcgctcc gccttttcgg ccggggtgat ggtgtaggta tcggatccgg ttgaagtcca
     3301 ggcggcagca tccgctacag gcaggtagga gcgagatgtg acctggatcg attcggctac
     3361 catgaaagcc gaataaaaac caaccccaaa ctgaccgatg agattggcgg cagctttacc
     3421 gctttcctgg accgcctcca tgaatttgcg cgctcccgag tgggcgatgg tgcccaggtt
     3481 ttccgccagc tcggtggcag tcatcccgat gccggtatcc tgaatggtca gggttttatt
     3541 ttctttgtcc gggaagacgc ggattgccag ctccaggtcg gggtcggcga cgtcccggtt
     3601 ggtaagcagc tcaaaatcca gacgggtgag ggcgtcagct gcgttggaga gcagctcgcg
     3661 cagaaaaatc tcacgttcag tgtatagtga atggatcagg atatccagta gttggcgggt
     3721 ttcggctttg aaggggatgc ctgctggatc ggcaggtgtg gtttgtggtt tgtccatcgt
     3781 atttgcctca tttattttgg taattgcccg gctgtttcta ccttccaggc gcgttcttaa
     3841 tataaccgag aatgataaga atcatgtaaa caggttgccc ttaattcggc aatttttccc
     3901 gctgcatcct tattcggcat cccagggcgc agtgtgtccg gtttgcccat taagggaggc
     3961 tcggatcggc tcgcccagct cgggcagcac gtcggcaact gcagccagta gaccgaaaca
     4021 gccggcgatc atcatatccc cgaacgggac agcaaaatgc gggcgcagtt ttgagtctcg
     4081 catgaggctg cgtcgcggtc cggtgacggc aatcccaaag ggaccggtgc ttaaatctat
     4141 gggttggggt tcataaatga gcgccccgtg cccgtgatgc ccgaacacat cggcgtgggt
     4201 taagctacca tccgccagtg tggttagcag cgcctctaat tgaggcaggt ccaacagacc
     4261 ggtatgatgc tcaaacacgc cctcgatgcc agtagggccc agacggaagg ccagggtgtg
     4321 gaaattgccc acgttggcga tgatcttacg cggggcggcg gcgaccagcg gatcgtaggt
     4381 ggcgcccagc acagcagctg gggcggtatc cattactacc agggggatct ccccgagggc
     4441 ctgcgcggct tcctggcggg cagactgcac cacggcttgc aggcgggtca tgatcggcgg
     4501 gacttctgcg gcgcgaaagg caaacgcgct caggcggttt tcagcccgca ggcgctcgtc
     4561 caggtaatcg aagcggaact ggcggtcgga tacatccggc ggggcagccc cgtggtcgaa
     4621 gactgctaca gctatggctg ccagctcttt gagcgagacg ccgaactgtt cgaaagcgat
     4681 tgccagagcg gtaaaatcga agtcgcgcag ctgcaggcgc agcacagaat ccggcagctt
     4741 actggcttca tcttcgctga cgacctcgat cccgctctgg ctgacaacct ccaggtcgtc
     4801 gttgaaggag cgggctgcgt ccggggtagc ataaatgggc agcccctgcc gggcgtgttc
     4861 ctcggccgcc caggcgctcg gcccgccgcc catggtcacc ccttccagca ggaccgcctg
     4921 gcgctggcgg gtcgcctggc gcaggcggcg gtggacgatc atggtggggg atggcaccac
     4981 cagcttgaac ccgttttcca ggtccaggcg cgagtcgtag agaaaaacgt cctgcgtgcc
     5041 ggtgccaacg tctaccgtta aga
//
LOCUS       DATMXK010000267         6403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_154513, whole genome shotgun sequence.
ACCESSION   DATMXK010000267 DATMXK010000000
VERSION     DATMXK010000267.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154513"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3185
                     /locus_tag="VN363_04885"
     CDS             <1..3185
                     /locus_tag="VN363_04885"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWQ83877.1"
                     /translation="QLRYQNGFDCQGLWVEVEVEKEMGFRSKKDIEAFGLEQFVRKCK
                     ARVLRYAAVQTEQSIRLGYWMDWNDPERLRWLAEKLLEDQTQTITVPGPDGPVTGSVE
                     QVVSQLGMPALGGSYFTFSNENNYMIWTFLKKCWERGWVYRGADVMPWCPRCATAISQ
                     HEIVTDGYAELTHDSVTLRLPLRQRPNESLLVWTTTPWTLTSNVAAAVGPELTYVRVR
                     QGDQIYYLSKGTLHMLRGAYEILGEIKGKEMEGWTYAGPFDELPAAQEEGGHTDLRKL
                     VAGVHQSAADVHQVILWDEVGEAEGTGIVHIAPGCGAEDFQLGKTRNFPMIAPLDDEG
                     HFISGFDWLVGQHVSEVSRPIFADLERKGILYHVEPYTHRYPTCWRCKSELVFRLVEE
                     WYISMGEVYDKPREALTAEEKSHSLRYQIMDVVDQIRWIPEFGYSREMDWLRNMHDWL
                     ISKKRYWGLALPIWVCGECDHFEVIGDEHELQGRASSGWESFAGHSPHRPFVDEVKLT
                     CPECNGTMTRIPDVGNPWLDAGIVPFSTLNYRTDREYWQSWYPANWISESFPGQFRNW
                     FYSLLAMATVVDNSAPFLQNFGYATLLAEDGRPMHKSWGNSIEFNEAADKMGVDVMRW
                     LYSAHKPENDLLFGYNRGDETRRRFLIPLWNVYTFVVTYARLDGWEPAFAFDPAEPEG
                     PTPTSDNLLDRWALARLNQVIARVTEALEDSDPLSASQAIEALLDDLSNWYVRRSRRR
                     FWKSELDSDKNTAYATLYHILVKLNKLLAPFIPFLTETMYQNLVRSVRPEAHTSIHHT
                     RWPAADLTCVDENMLEQMVLARQIASLGLSARNTAGLKVRQPLARVLVYAGGKANLSP
                     ELIEIVKDELNVKGFEFVEHAQKLVSYRVMPDNKLLGPRFGAQFPQVRAALAALDPGE
                     VALAVTSDSPVRLEVNGDMVELAPAEILVSTHPGEGLAVAADKLATVAVEAHITPELK
                     AEGLAREIVRRLQAMRKDAGFDISDRITAYYQAGPEVGEVFQKWQKYIMAETLSTALL
                     AGAAPQDAYTETQKVDGDSLVLGVLRAQRN"
     gene            3268..4443
                     /gene="alr"
                     /locus_tag="VN363_04890"
     CDS             3268..4443
                     /gene="alr"
                     /locus_tag="VN363_04890"
                     /EC_number="5.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00492.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008784 - alanine racemase activity
                     [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine racemase"
                     /protein_id="HWQ83878.1"
                     /translation="MQSINHATITTSGVVRPTQVEVNLDRLEENYRAIQLKVSPAKVM
                     PILKANAYGHGMVKVAEKMVSLGAEYFGVAVLEEGILLREQGIQTPILVLGGILGNQV
                     PLFLKYGLTLTASSVEKLDQVEQAAQALGVQAKVHLKIDTGMERIGVHYYNAESLLEA
                     SLKCRHVHVEGIFSHFANADAADLSHARLQLERFNEVLRFYARHSLPPPMRHMANSAA
                     ILQLPESTFDLVRPGILMYGVYPSPECERTVQVHPALSWKSHIVYFKVVLPGHPVGYG
                     STWQSDHMVRLVTVPVGYGDGYFRSMSNKAQVIIRGNKYPVVGRIAMDQITVNIEWDS
                     AYNDDPVILIGRDGDQVITCEDLAIWAGTIPYEILTNINTRVPRLYIETPPTTTVTT"
     gene            4525..5058
                     /locus_tag="VN363_04895"
     CDS             4525..5058
                     /locus_tag="VN363_04895"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ83879.1"
                     /translation="MAITRNKEIRSAASPDNLAVETILEVYWSRICRSLFSLLGDWDE
                     AEDLALETFLRWHQQPPRDTTNPGGWLYRVATHLGLNALRARQRRQHYETLAEAPGLE
                     TDPADLAAREQEAARVREILSQLKPRSAQILLLRHSGMSYAEIARELAVAQASVGSLL
                     ARATQEFESLYRNLEGD"
     gene            5061..6233
                     /locus_tag="VN363_04900"
     CDS             5061..6233
                     /locus_tag="VN363_04900"
                     /inference="COORDINATES: protein motif:HMM:NF025643.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zf-HC2 domain-containing protein"
                     /protein_id="HWQ83880.1"
                     /translation="MHPDEGILQAYIDAELTGSDHQQVAQHLATCSSCRNKLADIQDR
                     LSHIQQSLNQLNLAPPLPRLSTSAARARLVAHLSEQEKPNMFQRIFSRKLRPVWTGGL
                     IIAVLAIALAFPSVRVIANNFLGLFRAERITVVPVDENQLSERLDSSNQLESLFSDSV
                     SYQGGGEIQKATDVQNASEMANLTVRLPASPESPSQLFVQPGGSFSMVLDVRQARAVL
                     EEIGQDEVNIPDTLDGEIVSVDIPDMVMAHYGDCYDPTAPREKVDPDQAVIETGKSCM
                     ELFQIHSPTVNAPPDLDIIQLGQAMLQVLGMSPEEAAQFSATVDWTTTLVLPVPRSMT
                     TYQNISVDGVPATLIVQGNPQDPYHYMLVWIKDGVVYGLSGDNNIDTAIEMANSMQ"
BASE COUNT         1485 a         1761 c         1776 g         1381 t
ORIGIN      
        1 accaattgcg ttaccagaat ggctttgact gccagggatt atgggttgaa gtcgaagttg
       61 aaaaagaaat ggggttccgc agcaaaaaag atatcgaagc atttggactg gaacagttcg
      121 tgcgcaagtg caaggcgcgc gtgctgcggt acgccgccgt acagactgag caatccatcc
      181 ggctgggcta ctggatggac tggaacgatc ccgaacggct gcgctggctg gcggagaaac
      241 tccttgagga tcagactcaa acgatcactg tgcccggacc agatggcccc gtaactggca
      301 gcgtcgagca ggttgtaagc cagctcggca tgcctgcttt aggcggaagt tattttacgt
      361 tctccaacga gaacaattat atgatctgga cgttcctcaa gaaatgctgg gaacgcggtt
      421 gggtttaccg tggggctgat gtaatgccct ggtgcccacg ctgcgctacc gccatcagcc
      481 agcatgaaat tgtcaccgac ggctatgccg agctcactca cgacagcgtc accctgcgtt
      541 taccactccg ccagcgcccc aatgaatccc tgctggtatg gaccacaacc ccctggacat
      601 tgaccagcaa tgtggcagct gcagtcggac cggagctgac ctatgtacgt gttcgccagg
      661 gagaccagat ttattatcta tctaaaggca ccctgcacat gctgcgcggc gcctatgaga
      721 tactgggtga aataaaaggc aaagagatgg aaggctggac ctacgctggt ccattcgatg
      781 agctccctgc cgcccaggaa gaaggcggac atacggacct acgcaagctg gtcgctggcg
      841 ttcaccaatc cgctgccgat gtccatcagg tgatcctgtg ggacgaagtt ggcgaagccg
      901 agggtaccgg tatcgtccat atcgctcctg gctgtggtgc ggaggatttc cagctcggta
      961 aaacccggaa tttcccgatg atcgcccccc tggatgatga aggtcacttc atttctggtt
     1021 ttgattggct ggttgggcag catgtatctg aggtatccag accaattttt gccgatctgg
     1081 agcgcaaggg aatcctgtat cacgtcgagc cctatactca ccgttacccc acctgctggc
     1141 gctgtaaatc cgagctcgtt ttccggctgg ttgaggaatg gtatatcagc atgggcgaag
     1201 tgtacgacaa accccgggag gccctcaccg cggaagaaaa atcccacagc ctgcgctacc
     1261 agattatgga tgttgtcgat caaatccgct ggatccctga atttggttat tcacgcgaga
     1321 tggattggct gcgcaatatg catgattggc tgatcagtaa aaagcgctac tggggattag
     1381 cgctgccgat ctgggtgtgc ggcgaatgcg atcattttga ggtgatcggt gacgagcacg
     1441 agctgcaagg acgcgcctct tctggatggg aatcctttgc cgggcattcc ccacaccgcc
     1501 cgttcgtgga tgaagtgaaa ctcacctgcc cggaatgcaa tggcaccatg acgcgcattc
     1561 ccgatgtagg caatccctgg ctggatgcgg ggattgtccc cttcagcacc ctcaattacc
     1621 ggactgatcg cgagtactgg cagagctggt accccgccaa ctggatcagc gaaagcttcc
     1681 cgggccagtt ccgcaactgg ttctacagtt tgctggcgat ggccaccgtg gtagataaca
     1741 gtgccccatt cctgcagaat tttggttacg cgaccctgtt ggcggaagat ggccgtccca
     1801 tgcataaaag ctggggaaac tcgatcgagt tcaacgaagc agccgataag atgggcgtgg
     1861 acgtgatgcg ttggctgtac tcggcgcata aacccgagaa tgatctgctg tttggctata
     1921 accgcggcga tgaaacccgg cggcgcttcc tgatcccact gtggaatgtc tacacctttg
     1981 tggtcactta cgcccgcctg gatggctggg agccggcgtt tgcgttcgat cctgctgaac
     2041 ccgaaggccc aacacccacc agcgacaatt tactggaccg ctgggcattg gcccggctga
     2101 accaggttat cgcccgggta acagaggcac ttgaagactc tgatccgctc agtgccagcc
     2161 aggcgatcga agcactgctg gatgacctga gtaactggta cgtaaggcgc agccggcggc
     2221 ggttctggaa atccgaactg gacagcgata aaaacaccgc ttacgccacc ctgtaccata
     2281 tcctggtcaa gctcaacaag ctgctggcgc cgttcatccc cttcctgact gaaaccatgt
     2341 accagaatct ggtacgctca gttcgcccgg aggcacatac cagcatccat cacacacgct
     2401 ggcctgcagc cgatctcacc tgcgtggatg agaacatgct ggaacagatg gtccttgctc
     2461 gccagattgc cagcctgggt ttaagcgccc gtaacacagc cgggttgaaa gtccgccagc
     2521 ctctggcgcg ggtcctggtc tatgctggcg ggaaagccaa tctcagccct gagctgatcg
     2581 agatcgtcaa agacgagctt aacgtgaaag gctttgaatt tgttgaacat gcccaaaagc
     2641 tggtcagcta ccgggtcatg cccgataaca agctgcttgg gccgcgcttt ggtgctcaat
     2701 tcccacaggt ccgggctgcc ctggctgcct tggatcccgg tgaggttgca ctggcagtta
     2761 ccagcgacag cccggtgcgg ctggaggtaa acggagatat ggtcgaactg gctccagccg
     2821 agatcctggt cagcacccac cctggggaag gactggcagt cgcagcagat aaactggcga
     2881 ccgtggctgt ggaggcacat atcactcccg agctcaaagc cgaaggcctg gcgcgtgaga
     2941 ttgtgcgccg cttacaggcc atgcgcaaag atgctggctt cgatatctcc gaccgcatta
     3001 cagcctatta tcaagctggg ccagaggtag gtgaagtatt ccagaaatgg cagaaataca
     3061 ttatggctga gacactgagc accgcacttc tggcaggtgc agccccacaa gacgcctata
     3121 cggagaccca aaaggtcgat ggggatagtc tggtcctcgg cgtgctgcgc gcgcagcgta
     3181 attgagttac cggttaccag catagatgga gggctgctct gatgtggagc agccctcttt
     3241 cgttcagaat ggagtaaaat tccgcctatg caatccatca atcatgccac gatcaccacc
     3301 agtggggtag ttcgccctac tcaggttgaa gtcaacctgg accggctgga agaaaactac
     3361 cgcgctatcc agctgaaagt atccccggca aaggtgatgc cgatccttaa agccaatgcc
     3421 tacgggcatg gaatggtgaa agtcgccgaa aaaatggtgt ccctgggcgc agaatatttc
     3481 ggtgtggcag tgctggagga gggaatttta ctgcgggagc agggtatcca gacgccgatc
     3541 ctggttttag gtggcatcct gggcaaccag gttccgcttt tcctgaaata cggcctgact
     3601 ttgaccgctt catcagttga aaagcttgat caggtggaac aggctgctca agcgttgggt
     3661 gtacaggcaa aggtccatct caagatcgac acgggcatgg aacgcatcgg ggtgcattac
     3721 tacaatgccg aaagtttgct ggaagccagc ctgaaatgca ggcatgtcca tgtcgagggg
     3781 atattctccc attttgccaa tgcagacgca gctgacctga gccatgcgcg cttacaattg
     3841 gaacgattta atgaagtcct gcgcttctac gcaaggcaca gcctgccccc tcccatgaga
     3901 catatggcaa actcagctgc aatcctgcag ctccccgaga gcaccttcga cctggtccgg
     3961 ccaggcatct tgatgtatgg ggtctaccct tcccccgaat gcgagcgtac ggtgcaggtc
     4021 cacccggcac tgtcctggaa gtcgcacatt gtatatttca aagttgtcct tcccgggcat
     4081 ccggtaggct atggctctac ctggcaaagc gaccatatgg tccgtttggt gacagttccg
     4141 gtaggctacg gggatggtta ttttcgcagc atgtccaaca aggctcaggt gatcatccgc
     4201 ggcaataaat atcccgtcgt gggacggatc gccatggacc agattacagt taatattgaa
     4261 tgggattcag cctacaatga cgacccggtc atcttgattg gtcgcgatgg agatcaggtg
     4321 atcacctgtg aggatctggc gatctgggcc ggcaccatcc cctatgaaat tctgaccaac
     4381 atcaataccc gtgtaccacg cctgtatatt gaaacgcctc ccacaacgac tgtaactacc
     4441 tgatttctcc ccagccagta cccgtcacaa atctgattcg accggtgttg atattatgag
     4501 cgtgtaacac acgcaaaagg aactgtggca atcacaagga acaaggaaat tcgcagtgct
     4561 gcctcgccgg acaacctggc tgtagaaacc atcctggagg tttattggtc acggatctgc
     4621 cggtcgctct tcagcctgct tggcgactgg gatgaggctg aggacctggc attggaaacc
     4681 ttcctgcgct ggcaccagca gccgccccgg gatacaacca acccgggagg ttggctctac
     4741 cgggtagcga ctcatctggg cttgaatgca ctccgcgccc gccagcgccg gcagcactac
     4801 gaaactctgg ctgaagcgcc agggctcgaa accgaccctg ctgacctggc agcgagggag
     4861 caagaagcag cccgggtgcg cgaaatactc agccagctga aaccccgctc tgctcagata
     4921 ctgctcctcc gccattcggg gatgtcgtat gcagagattg ccagggagtt agctgtggcg
     4981 caggcctccg tgggcagctt gctggcccgt gccacacagg aattcgagag cctgtaccga
     5041 aatctcgaag gagattgagc atgcatccag acgaaggaat cttgcaagct tatatcgatg
     5101 ccgaattaac ggggagcgat caccagcagg tcgcccagca cctggcaacc tgctccagct
     5161 gccggaataa gctggctgat atccaggacc gcctgagcca tatccagcaa tcccttaatc
     5221 aactgaatct tgctccaccc ttacctcgcc tatcaaccag cgcggcgcgt gctcgcctgg
     5281 tggcgcatct gagcgaacag gagaaaccaa atatgttcca aagaatattt tccagaaagc
     5341 tgcggccggt ctggaccggt gggttaatca ttgcagtcct cgcaatagct ctggcttttc
     5401 catccgtgcg ggtcatcgcc aacaatttcc tgggattatt ccgtgccgaa cggatcacag
     5461 tggtcccagt cgatgagaac cagctctctg agcggctgga ctcttcgaac caattggaga
     5521 gtttgttttc ggattctgtg tcttatcagg gcggtggcga aatccaaaaa gccacagacg
     5581 tacaaaatgc cagcgaaatg gcaaacctga cggttcgcct gccagcatcc ccagagtctc
     5641 cttcccagct ctttgtacaa cctggtggaa gcttctccat ggtgcttgac gtccgccagg
     5701 cacgggcagt cctcgaagaa atcggacagg acgaggtcaa tattcccgac accctggatg
     5761 gggagatcgt gtcagtggac atcccggata tggtgatggc tcattatgga gattgctacg
     5821 atccgactgc tccacgagag aaagttgatc ctgatcaagc agtgattgaa acgggtaaga
     5881 gttgtatgga gctgttccaa attcacagtc ccacggttaa tgcgccaccg gatctggata
     5941 tcatccagtt aggtcaggca atgttacagg ttctgggtat gagcccggaa gaagcggctc
     6001 aattttctgc cacagttgac tggactacca ccctggtcct gcctgtgccc cgctctatga
     6061 ctacctatca gaatatcagc gtagatggcg ttccggccac actgatcgta caggggaatc
     6121 cccaggatcc ataccactac atgctggtgt ggattaaaga tggcgtggtc tatggactat
     6181 cgggtgacaa taatatcgat actgcgatcg agatggccaa ttcgatgcag taaatgagaa
     6241 tcgatctgcc ggggatcgcc agaaaacccg atccccggcg gatttattcg ccgtataatt
     6301 ccaagcatga taccctcatt accggaatca ccggtcatcg aaacacgccg cctgcggaaa
     6361 gtctttgatg ataaagttgc tgtcgatgat ctctccctta ctg
//
LOCUS       DATMXK010000268         4691 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_166642, whole genome shotgun sequence.
ACCESSION   DATMXK010000268 DATMXK010000000
VERSION     DATMXK010000268.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4691)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4691)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4691
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166642"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..568)
                     /locus_tag="VN363_04905"
     CDS             complement(<1..568)
                     /locus_tag="VN363_04905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558880.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate dehydrogenase subunit
                     beta"
                     /protein_id="HWQ83881.1"
                     /translation="MAKELLKGNVAVAEAAIRSGLQAYFGYPITPQTELLEWMAARMP
                     ELGRTFLQAESEVAAINMVYGAACTGARVMSSSSSPGVSLMMEGISYIAGTELPVVLV
                     DVVRGGPGLGNIAPSQGDYYQITKGGGHGDYFPIVLAPSNVQESIDLTVLAFDLAEKY
                     RSIVVVMSDGNVGQIMEPAELPPFQPIKT"
     gene            complement(580..828)
                     /locus_tag="VN363_04910"
     CDS             complement(580..828)
                     /locus_tag="VN363_04910"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558879.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ83882.1"
                     /translation="MPVKGWIEVSDHFCKGCDLCVSNCPQHVLALDLEHLTSKGYHPA
                     QLINADGCTGCAICAIVCPEAAITVYRDAPVRTAAGSV"
     gene            complement(978..2372)
                     /locus_tag="VN363_04915"
     CDS             complement(978..2372)
                     /locus_tag="VN363_04915"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycoside hydrolase family 13 protein"
                     /protein_id="HWQ83883.1"
                     /translation="MPVPYWVQDAVFYQIFPDRFANGDPHNDLSYLEPWGSPPTQHGF
                     QGGDLRGIINRLDYLIDLGVNALYLNPIFLATSNHRYNTTDYFKIDPRLGTLADFRAL
                     LEAAHSNHMRVILDGVFNHSGRAFFAFSDVLENQDRSIYQEWYHIKRFPVDAYTPGDS
                     VSYLGWWGLKSLPKFNTINPQVRKYLLSVARYWIDQGIDGWRLDVPNEIDDDSFWSEF
                     RATVKSANPDAYLVGEIWTADTRWVGDTHFDGLMHYPLRETLLDYLNGSFMTATQFAD
                     KVEGFLTLYPRSNVYAMYLPLGSHDTERLMTVLKDIRKVRLAFAFQFAYPGSPAVYYG
                     DEIGLEGGKDPDCRRAFPDDPAAWNKDLRNWVQLLATLRRQLAPLRRGGFRRLLLEDK
                     RRIYVFVRSLGREHVVVALNCGPVRRNMQIPISQLDWDDGRIVRNLLNREEFFVSGDK
                     LILNLEPWEAAWIG"
     gene            complement(2395..3744)
                     /locus_tag="VN363_04920"
     CDS             complement(2395..3744)
                     /locus_tag="VN363_04920"
                     /inference="COORDINATES: protein motif:HMM:NF013697.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWQ83884.1"
                     /translation="MVYYNPWQAEWGSRPDFTAWGSSMKKIGSLLSLFVLLVGLAACT
                     DIPATTPATSQPAQTEPAGSTRTPRPTATLTPPVFQGSISIWHSWDEAQLPALLEIIA
                     GFQETYPGILFDVLYVPAANLLPRYTQAASEGGGPALVLAPAEGGPDLFERDLVADLS
                     GSVPDELLQRLSPPALGAAQYHAALIGVPYSQRGVVLYRNRRIIPERPETLADLIQLS
                     QTFTEGEQIGAILERAPYYAGGHLFGFGGSFLDADGNPMFNTPAGLAWVELLQTFAQA
                     GPPDYFTDNDLDLFSQARVGFVIGGTWNRFALAEAIGEDFLEIDPWPVHDQGALMGFV
                     QTDNLFLNTNTSDAGRGLAALFIAHMLSDSTQMRLGESGLIPVVQVSLEDKLANQALT
                     ALAGGVAYPIQPEFSAFLLPLDQALLSVFDSGVPADQALLTAEEAVRNALANLTPTP"
     gene            complement(3815..>4691)
                     /locus_tag="VN363_04925"
     CDS             complement(3815..>4691)
                     /locus_tag="VN363_04925"
                     /inference="COORDINATES: protein motif:HMM:NF012958.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="alpha/beta hydrolase-fold protein"
                     /protein_id="HWQ83885.1"
                     /translation="LTLPADAYVEYAYFENPSEDKRLVDPLNPRKTTNGYGKYNHYFY
                     MPERHPNSLTLRRKGTPKGSLVHASLPTQGLISGSERKVHLYRPAAPGPYPLVLVWDG
                     QDFLRRGRLPVILDNLINQGEIPPLALALVESRGAVRMLEYASSDFSLAYTLEGVLPF
                     ARQHLDLLDLQKHPGAYGVLGASMGGLMALYTGLRLPGIFGKVLSLSGAFSFPELEMV
                     VFDLARLIDPDALRIWMNVGVYDILGLPNSNRQMAALLEERGFDFHFKEYLGGHNYTA
                     WRDELGAGLIYLFGQ"
BASE COUNT         1133 a         1329 c         1303 g          926 t
ORIGIN      
        1 ctgtttttat tggttggaag ggcggcagct cggccggctc catgatctga ccaacattgc
       61 catcggacat gaccacgaca attgaacgat atttttccgc caggtcaaag gctaacaccg
      121 tcagatcaat cgattcctgg acattggagg gcgccagcac gatcgggaaa taatccccat
      181 gcccaccacc tttcgtgatc tggtaatagt caccctgcga aggggcaata tttcccagac
      241 caggcccacc gcgcaccaca tccaccagca ctaccggcag ctcggtcccg gcgatataag
      301 aaatgccttc catcattaag ctaaccccgg ggctggagga tgagctcatc acccgcgccc
      361 cggtacaggc agccccatac accatattaa tggccgcgac ttcactctct gcctgcagga
      421 aggtgcgtcc cagctcgggc atgcgcgccg ccatccattc cagcagctcg gtttgggggg
      481 taatcggata accgaaataa gcctgcagcc cggagcgaat ggctgcttcc gccaccgcca
      541 cattgccttt gagcaattct tttgccatcc agcgcctccc tatacagacc cggcagcggt
      601 gcgcacggga gcatcccggt acacggtgat cgccgcttca gggcagacta tcgcgcaaat
      661 cgcacagccg gtgcagccat ctgcattgat gagctgcgca ggatggtagc cttttgaagt
      721 gagatgctca agatctaacg ccaggacgtg ctgcgggcag ttgctgacgc acagatcaca
      781 gcctttacag aaatgatcgc tgacttcaat ccatccctta acgggcatta cagcctcctt
      841 aaataaaaac aggttgggga ttcggtgaaa atcacacctt tattatccta ttcccaacct
      901 gatctgcaca gtgttagttg tcataaatgc aggatacaaa tgtcctgata acccagccag
      961 aaagccgtgg acggggttca gccgatccag gcagcttccc agggctccag attcaggatc
     1021 aatttatctc cagaaacgaa aaactcctcc cggttaagca ggttgcggac gatgcgtcca
     1081 tcatcccagt ccaattggct gatggggatt tgcatattac ggcgtacagg tccacagttc
     1141 agcgccacca cgacatgctc ccgtcccagg ctgcggacaa atacatagat ccggcgctta
     1201 tcttccagca gcaggcggcg aaacccgccc cgccgtaagg gggcaagctg gcggcgcaga
     1261 gtcgccagca gctgcaccca gttccgcagg tccttattcc aggctgccgg atcgtcggga
     1321 aaggcgcgcc ggcagtcggg gtcttttcca ccttccagac cgatctcatc cccgtaatat
     1381 acggccgggc tccccggata agcaaactgg aaggcgaacg ccaggcgcac tttgcggata
     1441 tctttaagca cggtcatcag gcgctcggta tcatgcgagc caagcggcag gtacatggca
     1501 tatacattgc tgcgggggta gagggtgaga aatccttcta ctttgtcggc gaactgggtg
     1561 gcagtcataa aactgccgtt aagataatcc agcaaggtct cccggagggg ataatgcatt
     1621 aagccatcga agtgggtgtc gcccacccag cgggtatctg cagtccagat ctcgcccacc
     1681 agataagcat ccgggttggc tgatttcaca gtcgcacgga attctgacca gaagctatcg
     1741 tcatcaatct catttggaac gtccagccgc cagccatcaa taccctgatc gatccagtag
     1801 cgggcaacgc tcagcaggta cttgcgtacc tgggggttaa tggtattaaa tttgggcagg
     1861 cttttgagcc cccaccaccc cagataagaa acggagtctc cgggggtata ggcatccacc
     1921 gggaagcgtt tgatatggta ccattcctgg tagatcgagc ggtcttgatt ttccagcaca
     1981 tcgctaaagg caaagaaagc ccgcccgctg tggttgaaga ctccatccag aataacccgc
     2041 atgtgattgg aatgggcagc ctccagcagt gcacggaaat ctgccagggt tcccaggcgg
     2101 ggatcaatct taaagtaatc cgtcgtgttg tagcggtgat tggaggtggc taagaatatt
     2161 gggttcaggt aaagcgcatt cacacccaga tcaatcaggt aatccaggcg gttgatgatc
     2221 ccgcgcaggt ctccgccttg aaacccatgc tgggtaggcg gtgagcccca gggctccaga
     2281 tagctgaggt cattgtgcgg gtcgccgttg gcgaatcgat cgggaaaaat ctggtaaaaa
     2341 accgcgtctt gcacccaata ggggacaggc ataaaacctc cagtgaaaac cgggtcaggg
     2401 agttggggtc aaattcgcca gggcgttgcg gacggcttct tcggcagtga gcaaggcttg
     2461 atcagcaggc acgccgctgt cgaacacgga caggagcgcc tggtccaaag gcaggagaaa
     2521 ggctgaaaat tcgggctgga tcgggtaggc aaccccacct gccagggcag tgagcgcctg
     2581 gttggccagt ttgtcttcca gggatacctg gacaaccggg atcaggccac tctctcccaa
     2641 ccgcatctgg gtagagtctg agagcatatg ggcgataaat aacgctgcca gcccgcggcc
     2701 ggcgtccgat gtattggtat tcaggaacag gttatctgtc tggacaaagc ccatcaacgc
     2761 gccctgatcg tgcaccggcc aggggtcgat ctccaggaaa tcctcgccaa tcgcctctgc
     2821 cagagcaaag cggttccagg ttcccccgat cacaaaaccc acccgagctt gcgagaataa
     2881 atccagatcg ttatccgtaa aataatcggg cggtcctgcc tgggcaaaag tttgcagtaa
     2941 ctcaacccag gccaggccgg ccggagtatt gaacatagga ttgccatctg catccaaaaa
     3001 actacccccg aagccgaaca aatgaccgcc ggcatagtaa ggggcacgct ccaggatcgc
     3061 cccgatctgt tcaccttcgg tgaaagtctg ggaaagctgg atcagatccg ccagtgtttc
     3121 gggacgttct ggaatgatcc gccggttgcg atacagcacc accccgcgct ggctgtatgg
     3181 cacgccgatc aaggcagcat gatattgggc agcacctaat gctggcgggg agagacgctg
     3241 cagcagttcg tctggcacag agccgctgag atcggcgacg agatcacgct cgaaaagatc
     3301 tgggcctcct tccgccggag ccaggacgag cgccggacca ccgccctcgc tggcggcctg
     3361 ggtgtagcgc ggcaggagat tcgcagccgg gacatacagt acgtcaaaga gaatccccgg
     3421 gtaagtttcc tggaaaccag caataatttc cagcaaagcc ggcagctgcg cttcgtccca
     3481 ggaatgccag atactgatcg aaccttgaaa aacagggggt gtaagggtgg cggttgggcg
     3541 aggtgttcga gtggaaccag ccggctctgt ttgggcaggc tgggaagtcg ccggagttgt
     3601 cgcagggata tctgtgcagg ctgccagccc caccagcagc acgaacaagg acaggagcga
     3661 accaatcttc ttcatcgatg atccccaggc agtgaaatct ggccggcttc cccattcagc
     3721 ctgccagggg ttgtagtata ccaataagac tgatccctaa ttgtaccagc cagcagaccc
     3781 aacagccggt cctttacata tgctgccagg gtggctattg accaaataga tagatcaatc
     3841 cagccccaag ctcatcccgc caggcagtgt aattatgccc gcctagatat tctttgaagt
     3901 gaaaatcaaa gccccgttcc tcaagcaggg cagccatctg ccggttgctg ttcgggagcc
     3961 ccaaaatatc gtagacacca acattcatcc agatgcggag ggcgtcaggg tcgattagac
     4021 gcgccaggtc aaaaacgacc atttcaagtt cagggaagct gaacgctccg gaaaggctga
     4081 gcactttacc aaaaattccc ggcagccgca gaccggtata caacgccatt aacccaccca
     4141 tcgaagctcc cagaacgcca taggccccgg ggtgtttctg tagatcgagc aggtccagat
     4201 gctggcgagc aaagggcaga acgccttcca atgtatacgc cagagagaaa tcgctgctgg
     4261 catattccag catgcgcacc gcaccccggc tttcaaccaa tgccagggcc agaggcggga
     4321 tctccccttg attgataagg ttgtccagga tgaccggcag acggccgcgg cgcaggaaat
     4381 cctgaccatc ccaaaccagc accagggggt aaggacctgg agccgccggc ctgtataaat
     4441 ggactttgcg ctctgaccca gaaatcaagc cttgagtggg taacgaagca tggacgagac
     4501 tgcctttggg agttcccttt cggcggaggg taagcgagtt gggatgccgt tcaggcatat
     4561 aaaaataatg attgtatttg ccatagccat tggtcgtttt gcgcgggttg agaggatcga
     4621 ccaggcgctt atcctcgctg ggattttcaa aatacgcgta ttcgacgtac gcatccgcgg
     4681 gcagagtcag g
//
LOCUS       DATMXK010000269         5466 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161609, whole genome shotgun sequence.
ACCESSION   DATMXK010000269 DATMXK010000000
VERSION     DATMXK010000269.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5466)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5466)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5466
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161609"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            291..1148
                     /locus_tag="VN363_04930"
     CDS             291..1148
                     /locus_tag="VN363_04930"
                     /inference="COORDINATES: protein motif:HMM:NF014679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ83886.1"
                     /translation="MSEFKRKVAVVTDSTSSLTPAMGKQYGLHVIPEYVMFGDQSYYD
                     GVNLDAETFYRLLKNSKKLPTTSQPSIQDFVKLYSDLSEKAEAIVSIHISQKMSATLD
                     SARGACQQLPGVAIQVIDSQSVSMGLGVIAIAAARAAAAGKEVDEVVSLVEALVPKVN
                     VIFTVDTLEYLYKGGRIGGATALMGSVLNIKPVLYIKEGRIEPLEKARTKKRAIERVM
                     DLMAERVGSTEGLHAAIMHCNVPEEAHVLGEQVKARFHLDEPIVTEAGPIIGTHGGPG
                     TLGVVFYNE"
     gene            1388..1804
                     /locus_tag="VN363_04935"
     CDS             1388..1804
                     /locus_tag="VN363_04935"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83887.1"
                     /translation="MNRKKSARLPRKPRLLKPKTEHDCPVCQQHKSKATPSAQLPPVA
                     WSTRKGRGGPRKKISTQGFACPNPLCEYRGIRDEAIHALVGNGTHGKHAAIQDLKCQA
                     CGMKFTVRRNTVMYRLKHSPDTVTRVLWLLALGVGG"
     gene            1875..2450
                     /locus_tag="VN363_04940"
     CDS             1875..2450
                     /locus_tag="VN363_04940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83888.1"
                     /translation="MHELKHRLCSGSTPVFPSDGLKHYFYALTAHFGQWEPGQGKKPV
                     WVLLNQFAYAQVIKHQRRRRTVKVDRRLLWGDEQSYRSRLKQAGLSGRINTSFVERTN
                     LTIRQAVSKLTRRTWCPAHYLPELLEHLEWWRSCYHFARYHKSLAVELSYPIPRKGRQ
                     QPLRYRRRTPAMAAGLTTRRWSVSELISYPV"
     gene            3137..4321
                     /locus_tag="VN363_04945"
     CDS             3137..4321
                     /locus_tag="VN363_04945"
                     /inference="COORDINATES: protein motif:HMM:NF012707.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ83889.1"
                     /translation="MSRKLIIVFSVVISIGMLATACRGAQTEVPATATASTGEEVAPA
                     VETVEVIKTVIVEVPAAGEAQPPEVIQAAGYGETLQAILNRGQVVCGVHTELLGFGYL
                     DENGRNIGFDIDLCRAIAAAVLGDPEAIEAVPITAADRGPVLQTGEVDVVTRNMTWTS
                     KRDAEWGNFTWITFYDGQGFLVRADSGVTSLEEMDGATVCVTTGTTTERNLATAFGDA
                     GLTYEAVTFEETSAVYGAYEEGRCDVATSDKSQLAAVRAGFQNPDDHLILDITISKEP
                     LTPAVPTGDAQWLDIVKTVVWGLINAEEYGITSANVEEMKSSENADIRTLLGVEGESD
                     WGYGTLGLEADSLAQAIAAVGNYGEIYDRYFGAEGIAFTLDRGLNNLWTNGGLIYAPP
                     IK"
     gene            4404..>5466
                     /locus_tag="VN363_04950"
     CDS             4404..>5466
                     /locus_tag="VN363_04950"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="The N-terminal region of this protein, as described
                     by TIGR01726, is a three transmembrane segment that
                     identifies a subfamily of ABC transporter permease
                     subunits, which specificities that include histidine,
                     arginine, glutamine, glutamate, L-cystine (sic), the
                     opines (in Agrobacterium) octopine and nopaline, etc;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_process: GO:0015837 - amine transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWQ83890.1"
                     /translation="MVTDKYFHKKTPIWRDERVLKAAAQIISSLVVFGILYWAIGNFL
                     EFAQQRGMPLSYGFLDEAAGFPIKESFIPYESTMSFGKAVLVGLINTLVVSVLGILFA
                     TLLGFIIGLMRLSSNWLISRIALAYIEFHRNIPLLVLLFLWYFGVFTRLPDVKESITL
                     PGPTYINQRGFYLTWPHLSERGALFATSVAAGIILAIITWVVLRRIREDKGRETYFGR
                     ISLTVLILVPLIGWFASGGEPLWFDVPVLQGFNFQGGLRLTTEFAALLIGLAMYTAAF
                     IAEVFRAGILAVQKGQIEAASAVGLRYSQVLMLIIIPQAMRVIIPPMISQYLNLTKNS
                     SLALAIGYQELFAIGKVTIN"
BASE COUNT         1277 a         1493 c         1480 g         1216 t
ORIGIN      
        1 gcgctgcccc tgccgcgcct gatgtttctg ccgaaagaga cccgcgatca cctgcgagca
       61 gcccgccgcg agcatctcat ggccctgcgc agtatcttgg accaggccat tgagcaggta
      121 gaaaagaccg agaaaccaaa acacaaggca gagcgcgtca aggtggagta aggtcacact
      181 gcgacttacg ttcgtcttca aggagcactc ctctacgcct ggtagggtag agggcagttt
      241 attattgcca ggcgattaac tttcaaaaac cattagagca aggaggagcc atgtccgaat
      301 tcaaacgcaa ggtagctgtc gtcaccgatt ctacatcatc tctcacccca gccatgggga
      361 agcagtacgg cctgcacgtt ataccggaat acgtcatgtt tggtgatcag tcctactacg
      421 atggagtcaa cctggacgcc gaaaccttct atcgtctgct gaagaacagc aaaaaattgc
      481 caacaacctc tcaaccaagc atccaagatt ttgtaaagct ctattctgac ctatctgaga
      541 aggccgaggc gattgtctcg attcatatct cccaaaagat gagcgccacg ctcgattcgg
      601 ccaggggtgc ttgccagcaa ttgccgggcg tggcaatcca agtgattgac tcgcagtcgg
      661 tatccatggg tctgggcgtg atcgctatcg ccgcagcgcg tgccgcagct gccggtaaag
      721 aggtggatga agtcgtctct ctggtagagg cattggtccc caaggtgaac gttattttca
      781 cagtggatac gctggagtac ctgtataaag gcgggcgcat cggcggtgca actgccttaa
      841 tgggctcagt gctgaacatc aagcccgttc tgtacataaa agaggggcgg atcgaaccgt
      901 tagagaaagc gcgcaccaaa aagcgagcta tcgagcgggt gatggacctg atggccgaaa
      961 gggttgggtc aactgagggc ttgcacgccg cgattatgca ttgtaatgtc ccggaagaag
     1021 cacacgttct tggcgaacag gtgaaagcac gctttcatct tgacgagccg attgtcaccg
     1081 aagccggacc gatcatcggc acccacggcg gtccagggac gctgggtgtc gtgttctaca
     1141 atgagtgaat cgatggggct agaggcctgg agagaagttg aatcatcaag acgctgcgga
     1201 cggtccagcc acaatcggtg gtggataact gagtagcagc attacccttg tggtaagata
     1261 gcaggccata tttccaacga acaggaaaat agcctgccag atgaatcatc tcttcgaact
     1321 ccatggggtt ctgattgtcc tgatccaact ctatcgttgg ctgtatcgga tgtggttgca
     1381 atgctggatg aacaggaaga aatctgccag acttccccga aagccacgcc tgttaaagcc
     1441 caaaaccgaa catgactgcc cggtctgcca acaacataag agcaaagcaa ctccctcggc
     1501 acagctacct cccgtcgcct ggagcacccg caaaggacgc ggtggcccca ggaagaagat
     1561 ctccacccaa gggtttgctt gcccgaatcc cctctgcgag tatcgtggca tccgcgacga
     1621 agccatccat gccctggttg gcaatggcac acacggcaaa cacgcagcca ttcaggatct
     1681 caaatgccag gcgtgtggga tgaaattcac cgtgcgcaga aacacggtca tgtaccggct
     1741 gaaacattcc cccgatacgg ttaccagggt cttgtggttg ttggcactgg gtgtgggtgg
     1801 ttagcgatgc taccaccagg ctggtccctt ttctgcaggt tggctgtcgc tctcaggaga
     1861 tggcttaccc ggtggtgcat gagctcaaac acaggctttg ctctggctcc actcctgtat
     1921 ttcccagtga tggcttgaaa cactatttct atgctctaac tgcccacttc ggccaatggg
     1981 aacccgggca aggcaaaaag ccggtctggg ttttgctcaa ccagtttgcc tatgcccagg
     2041 tgatcaaaca tcaacgcagg cggcgcacgg tgaaagtcga tcgccgcctg ctgtggggtg
     2101 atgaacaaag ctaccgatcg cgcctcaaac aggctgggct gagcggcaga atcaacacgt
     2161 ccttcgtcga gcggaccaat ctcaccatcc gccaagcggt ttccaagttg acccgccgta
     2221 cctggtgtcc agcccactat ttacccgaac tgctcgaaca tctcgagtgg tggcgcagct
     2281 gctatcattt cgcccgttac cacaagagcc tggctgtcga actctcctat ccaatcccgc
     2341 gcaaaggacg ccaacagcca cttcgttacc ggcgcagaac cccagcgatg gcggccggcc
     2401 tgacaacccg ccgctggtca gtcagcgagt taatctctta tccggtgtga ttttctttcc
     2461 caccaatggg ggttttttat tacaattatg ggggtagtgc gactcttgcc tcggtgtgac
     2521 gccagcgagt ggcgttacaa tcccagaacg caccaaacag taaccgtttg agttatccac
     2581 cacggataaa ggtgggaccc ctatttgcat ttagcggaga gaggacccac ctctcggcgg
     2641 agtaagtcgt ggcattgtgg gtaatcaagg agattttggc ttcggttgcc aactgtacct
     2701 acccgaccgg taaacctcca gaatggatac aaatcatgat acacttgaac catcgcccaa
     2761 gaagatgcct tgatttccta tcgcctttcg aggtattctt tgatcattct gtttgcactt
     2821 gcagtttgaa accaggcttg cataacggcc gatttctatc tataattgta aaagttgcgt
     2881 agctgctata aagttagcgg gtggagagca gccacccata ctcccactaa gccaccgaac
     2941 agcgtaagtc ctggattgta gttcataagt aaacaatctc agcctgtgtg aatattatcc
     3001 acgttagttc caattaaatc atgccaggtt gacatagaac catctgaaag gaggtgcagc
     3061 atccagcctg atcactactc ctagtgcagc catacgtgat tgatcaatct atatacctca
     3121 caagaaggag agagacatgt cccggaaact aattattgta ttcagcgtcg taatctccat
     3181 cggcatgctt gctaccgcgt gccgcggcgc ccagaccgaa gtgccggcaa ccgccaccgc
     3241 cagtaccggt gaagaggtcg ctcctgctgt cgaaacggtc gaagtcatta agacagtcat
     3301 cgtcgaggtg ccagcagcgg gtgaagccca acccccagaa gtcatccagg cggctggcta
     3361 tggagaaacc ttgcaagcca tcctgaatcg cggccaagtc gtgtgcggcg tgcacaccga
     3421 gctgcttggc ttcgggtatc tggacgagaa tgggcgcaac atcggcttcg acattgacct
     3481 atgccgagcc attgctgcgg ctgtcctggg tgatcccgag gcaatcgagg ccgtcccgat
     3541 caccgcagcc gatcgcggcc cggtcttgca gaccggcgag gtcgatgtgg tcactcgcaa
     3601 tatgacctgg acatccaagc gcgatgcaga atggggcaac tttacatgga tcacgttcta
     3661 cgacggtcag ggctttctgg tgcgtgcgga cagcggggtc acctcgttgg aagaaatgga
     3721 tggcgctaca gtatgcgtga ctacgggcac aaccacggag aggaacctgg caacagcctt
     3781 cggcgatgct ggtctgacat acgaggcggt cacttttgag gaaacctcag ccgtttacgg
     3841 cgcctacgag gaaggccgct gcgatgtggc taccagcgac aaatcgcagc tagctgccgt
     3901 gcgcgccggc ttccaaaatc cggatgacca tcttatcctg gacatcacca tctccaaaga
     3961 accactcaca ccggcagtac cgaccggcga tgcccagtgg ctggatatcg tcaagacggt
     4021 ggtatggggg ttgatcaatg ctgaggagta cgggattacc tcagcgaacg tagaggaaat
     4081 gaaatccagc gagaatgcgg acatacgcac gctgctcggc gtggaaggag aatctgactg
     4141 gggctacggc actctcggcc tcgaggcgga ttcactggcg caagcaattg ccgctgtcgg
     4201 taactacggc gagatttacg accgctactt cggtgcagaa ggcatcgcct ttacactgga
     4261 tcgcggctta aataacctgt ggaccaatgg tggcttgatc tacgcgccgc cgatcaagta
     4321 atcgttatag acactcctgg ttgggtgtgc agcctgcttg ctaatggact gcacactcaa
     4381 cgcggggatc accaagacac ctgatggtaa cagataagta tttccacaag aaaaccccaa
     4441 tctggcgtga cgagcgcgtg cttaaagctg ccgcgcaaat catttcatca ctcgttgtct
     4501 ttggaatcct ttactgggcg attggcaact tcttagagtt cgcccagcag cgcggcatgc
     4561 cgctgagtta cggattcctg gatgaagctg caggtttccc gatcaaggaa tctttcatcc
     4621 cttatgaatc aaccatgtcc tttggaaaag ccgtcctggt cgggctgatc aacacccttg
     4681 tagtgtccgt tttgggaatc ctgttcgcca cactgttggg ctttatcatc ggtctgatgc
     4741 ggctctcctc caactggcta atcagccgca tcgcgctggc ttatatcgag tttcaccgca
     4801 acatccctct gctggtcttg ctgttcctgt ggtacttcgg tgtctttacc cggcttccgg
     4861 atgtaaaaga gagcattacg ctaccaggac caacatacat caaccagagg ggcttttacc
     4921 ttacctggcc ccacttatct gagaggggtg ctctattcgc aacctcggtt gcagctggta
     4981 tcatcctggc gatcatcacg tgggttgtac tacgccgaat ccgggaggac aaaggcaggg
     5041 agacgtattt cggcaggatc agcctgacgg tactgatcct ggtgccactg attggctggt
     5101 tcgcttctgg tggtgagccg ctatggttcg atgtcccagt gctgcagggc ttcaactttc
     5161 agggcggatt gcgcctgaca accgagtttg cagctctgtt gatcggccta gccatgtata
     5221 ctgctgcgtt tatcgctgag gtgttccgag ctggcatcct cgccgtgcag aaggggcaga
     5281 tcgaagcagc cagcgcagtg ggactgcgct actctcaggt tttgatgtta atcatcattc
     5341 cgcaggcgat gcgcgtcatc atcccgccaa tgatcagcca gtatttgaat ttgacgaaga
     5401 actcgagcct ggcactggcg atcggctacc aggagctctt tgcaatcgga aaggtcacca
     5461 tcaacc
//
LOCUS       DATMXK010000270         6221 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_148464, whole genome shotgun sequence.
ACCESSION   DATMXK010000270 DATMXK010000000
VERSION     DATMXK010000270.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6221)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6221)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6221
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_148464"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..998)
                     /locus_tag="VN363_04955"
     CDS             complement(<1..998)
                     /locus_tag="VN363_04955"
                     /inference="COORDINATES: protein motif:HMM:NF015882.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051287 - NAD binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malic enzyme-like NAD(P)-binding protein"
                     /protein_id="HWQ83891.1"
                     /translation="MSKITREDALEYHHLNGKPGKIAVVPTKPMANQRDLGLAYTPGV
                     AVPVLEIEKDPELAYEYTSKGNLVAVISNGTAILGLGDRGALAGKPVMEGKGVLFKKF
                     ADVDVFDIEVNSHDPDEIIRVVAAISPTFGGINLEDIKAPECFYIEETLKGMLDIPVF
                     HDDQHGTAIISGAALVNGLEVVGKNIADVRIVVSGAGASAISCSELMVRLGATRSNIM
                     LVDSKGVVYKGRQEGMNKYKERFTPETDARTLADAVRGADVFYGLSVADILTEAMVKT
                     MAEDPLIFVMANPDPEIKYELARAARPDAIVATGRSDYPNQINNVLGFPYIFRGAL"
     gene            complement(1172..4525)
                     /locus_tag="VN363_04960"
     CDS             complement(1172..4525)
                     /locus_tag="VN363_04960"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWQ83892.1"
                     /translation="MASGYYRFPTIQGETTVFSCEDDLWSVPASGGIARRLTASLSED
                     IYPLLSSDGQKLAFAGRDEGHSEIYLMPAQGGPARRLTFMGALTCQPVAWTAEGKLIF
                     ASTAGHWYQRFTQLYQIDPSSGAPELINYGLARHISFGPNGGVVLGRLSTEPARWKRY
                     RGGTAGQIWIDSSANGDFHHLIELGGNLANPIWLGERIYFISDHEGIGNLYSCLPDGT
                     GLRRHTDHDDYYVRNPSSDGQQIVYHAGADLYLYSPQSDISYPIAIDFHSPQVQRSRK
                     FVDPARYLEAWDLHPNGQSLAVTTRGKLFSFANWEGAVIQHGSDAEPLNSEMITTGIR
                     YRLPCWLPDGKRMVAVTDAGGEEHFVILHADGSAEPQPLEALDIGRPEAVACNPHKDQ
                     IVFSNHRYELLVLDLETKELIRIDRGISNPILGFSWSPDGKWVVYSASISLQRQAIKL
                     WNAVTREVTQITDPVLRDLAPSFEPHGLFIYFLSFRDFDPVYDNLQFDLGFPRGMRPY
                     LITLKKDQVSPFIPQPYTEPEDEMEESKDEEKQEIESEENLQDSETPLQKIPDITPPE
                     PPVEKEEEKKAPEIKEIEIDLEGIQERIIAFPVPEGRYRQVRGTRSGKVLYTRYPVDS
                     TLDESNDEERAARGNLYFYNYEDQKEEMLVSGVTAFDVNASGAWMAYLSRNHLRIMKA
                     GEKPMDDNMMPNRKSGWIDLSRLKVSIVPGAEWRQMFREAWRLQRDQFWTQDMSQVDW
                     LAVHDRYLPLIDRVSSRSEFSDLMWEMQGELGTSHAYEFGGDYRPQPRYQQGYLGAEY
                     HYHAESGGWQITRLLHGDPWSDVAQSPLLKPGMNVSEGDILISINGRKLNADISPGQT
                     LVNMADEEVFLAVRSSDDPTLRYVTVKALGRDEPLRYRAWVETNRLAVHTATQGKVGY
                     LHIPDMGPAGYAEFHRGYLSEIDRQGLIVDLRFNRGGHVSPLLLEKLARRRLGYDTAR
                     WSEIPNPYPPESVTGPIVALTNEYAGSDGDIFSHSFKMLKLGPLLGKRTWGGVIGIWP
                     RHSLVDGTQTTQPEFSFWFEDVGWGVENYGTDPDIEIDNTPQDYMQSSDAQLERAITE
                     ALKLLADNPPSLPDFSQRPDRSLPRLPPR"
     gene            4710..5645
                     /locus_tag="VN363_04965"
     CDS             4710..5645
                     /locus_tag="VN363_04965"
                     /inference="COORDINATES: protein motif:HMM:NF017206.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-binding protein"
                     /protein_id="HWQ83893.1"
                     /translation="MILVTGGTGFVGKQLIGQLTELGYPVRTMVSPSPRSPDLPRNVP
                     VEIAVTSLTDERGLRAAMVGVDTVYHLISGEWKGPRASLLKVDIQGTRAVVDAAKDAG
                     VKRIFYLSHLGADRASAYSVLKAKGIAEEFIRRSGLEYTIFRTALAYGQQDAFTTGIA
                     GILQTLPYFFPIPGDGRSLLQPIWVQDLVTCLAWALDDRGTINRLYEIGGPEYFTFKQ
                     VVEMVMDKIQLRRRFLHMRPTYLRWMTIALEAIFPNLPVSIYWLEYLATNRTCALDTV
                     PREFTLLPARMVHKTGYLQDQNWRSIILKSLWQRN"
     gene            5664..>6221
                     /locus_tag="VN363_04970"
     CDS             5664..>6221
                     /locus_tag="VN363_04970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83894.1"
                     /translation="MTGWALHILEEPEEMEAVERLQSQVWPGSETDIVPAHLLVTAAH
                     NGGLVIGASINPDGSEPGGASELVGFVFGFPGLYTTPDGPRPKHASHMLGVRPDFRNR
                     GVGFTLKRAQWQMVRNQGLDLVTWTFDPLLSRNAHLNITQLGAVCNYYRRNVYGSMRD
                     TVNAGLDSDRFQVNWWVNSKRVQRRL"
BASE COUNT         1439 a         1603 c         1643 g         1536 t
ORIGIN      
        1 tccagggctc cccggaaaat atatgggaaa ccgaggacat tgtttatctg gttgggataa
       61 tcagaccttc ctgttgccac gatggcatcc ggccgggcgg ctcgagccag ctcatatttt
      121 atttctggat caggattcgc catcacaaag atcaatggat cctctgccat ggttttgacc
      181 atagcttcgg tcaagatatc tgctaccgac aatccataga acacgtccgc cccacggaca
      241 gcatcggcaa gggtgcgcgc atctgtttcg ggagtgaagc gctctttgta cttattcatc
      301 ccctcctggc gacctttgta taccacccct ttcgagtcaa ccagcatgat attcgagcga
      361 gtagctccca ggcggaccat cagctcagag catgagatcg ctgaagcgcc agcacctgag
      421 acgacaatcc ggacatcggc gatgtttttt ccgactactt ccagcccatt aaccagggct
      481 gcgccggaga taattgcagt cccatgctgg tcatcatgaa atacggggat atcaagcatg
      541 cccttaaggg tttcttcgat ataaaaacat tctggggctt tgatatcttc caggttgatc
      601 ccaccgaaag tcggcgagat cgctgcaacg acacggatga tctcatctgg atcatgggaa
      661 ttaacctcga tatcaaatac gtcgacgtcg gcgaattttt taaacaatac gcctttgcct
      721 tccatcaccg gctttcctgc gagcgcaccc cggtcgccca aaccaaggat cgcggtgccg
      781 ttggaaatta ccgctaccag gttgcctttg gaggtgtatt cataggcaag ctctggatct
      841 ttctcgatct caagaaccgg aactgccaca ccaggggtgt atgccagtcc taagtccctc
      901 tggttcgcca tcggtttggt tggcacaacc gcaatcttgc ctggctttcc gttcaagtga
      961 tggtactcaa gggcatcctc acgggtgatt ttactcatct tttttctcct ttcaagcgag
     1021 atgaccccaa ttacttaccg ggcatgctga taactctatc atcgcgcaat cagcgcccgt
     1081 tgtgaagttg cgtttgtcat aatttgtaca agcaatagat ctattcctga agtggcttat
     1141 aagtcggatg gggtgagatg gtgaataaca atcatctcgg tggcaggcgt ggtaatgagc
     1201 gatcaggacg ctgggagaag tcgggtaggc tgggtgggtt atcggccaga agtttcagag
     1261 cttcagttat cgcccgctct agctgtgcat ccgaactctg catatagtcc tggggggtgt
     1321 tgtcgatttc gatatccgga tcggtgccat aattttcaac gccccaaccg acatcttcaa
     1381 accagaatga aaattctggc tgggtagtct gggttccatc taccagtgaa tgccgcggcc
     1441 agatgccgat cacccctccc caggtacgtt taccaagcag gggtcccagc ttgagcatct
     1501 taaagctatg gctgaagata tctccatccg atccggcata ctcgttggtg agggcaacga
     1561 tcggtccggt gacagattct ggcggatagg ggttaggaat ctcactccag cgggcagtat
     1621 catatcccag ccggcgccga gcaagctttt caagcagcaa gggggataca tgtcccccac
     1681 gattgaaccg taaatcgacg atcaatccct ggcggtcgat ctctgataga taaccgcgat
     1741 gaaattccgc atatccagct ggacccatat ccggaatgtg caaatatcca actttacctt
     1801 gtgttgctgt atgaacagcc aggcggttcg tctcaaccca ggcccggtag cgcagcggct
     1861 catctcgtcc cagcgccttc actgtgacat agcggagggt aggatcatcc gatgaacgca
     1921 cggccaggaa aacttcttca tccgccatgt tgaccaatgt ctgcccggga gaaatatcgg
     1981 cattcagctt gcgcccattg atcgagatta aaatgtcccc ttcggagaca ttcatgcccg
     2041 gtttcaataa aggtgactgg gcaacgtcac tccacggatc gccatgcaag agacgtgtga
     2101 tttgccagcc gcccgattca gcgtggtagt ggtactctgc acccaggtaa ccctgctggt
     2161 agcgtggttg gggacggtaa tctccaccga attcataagc atgggatgtg ccaagctcac
     2221 cttgcatttc ccacatcaga tcggagaact cggagcgact gctgacccga tcaattaatg
     2281 gcaggtaacg atcatgaaca gccagccaat caacctggga catatcctgt gtccagaatt
     2341 gatcgcgctg caatcgccag gcttcgcgga acatctggcg ccattctgca cccggcacaa
     2401 tggatacttt cagccggctg agatcaatcc aacccgactt gcggttaggc atcatattgt
     2461 catccatggg cttttcacca gccttcatta ttcgcaagtg gttgcgcgag aggtaagcca
     2521 tccatgcccc agaggcattt acatcaaagg ctgtcacacc agaaaccagc atctcttcct
     2581 tctggtcctc gtaattgtaa aaatacagat tgccacgcgc cgcacgttct tcatcattgc
     2641 tttcgtccaa tgtgctgtca accggatagc gggtatacag gacttttcct gagcgggttc
     2701 cgcgcacctg gcggtaccgc ccttctggga cagggaaagc aatgatccgc tcctggatgc
     2761 cctctaaatc tatctcaatc tccttgatct caggcgcttt cttttcttct tctttctcta
     2821 ccggtggttc tggcggagtg atgtctggga ttttttgcag tggggtttct gagtcctgca
     2881 agttttcttc cgattctatc tcctgcttct cctcgtcttt gctctcttcc atttcatcct
     2941 ccggctcagt atacggctga gggatgaagg gagagacctg gtccttcttc aatgtgatca
     3001 ggtaagggcg catgccgcgt ggaaaaccca ggtcaaactg aaggttatcg tagacagggt
     3061 cgaaatcgcg aaaggacaag aagtagataa ataacccatg cggttcgaag gaaggagcaa
     3121 gatcgcgcag gaccggatca gttatttgtg tcacttcccg cgtcacagcg ttccagagtt
     3181 taatcgcctg gcgttggaga gatatcgagg cgctgtacac gacccacttt ccatctggcg
     3241 accagctaaa acccaaaatg ggattggaga taccgcgatc gatgcggatg agctccttcg
     3301 tctccagatc taagacaagc agttcatagc ggtgattgct gaatacaatc tggtctttat
     3361 gagggttaca ggcaaccgct tccgggcgtc caatatccag cgcttccaga ggttgtggct
     3421 ctgccgagcc atccgcgtgt aaaatcacga aatgttcttc accaccggca tcggtaacgg
     3481 caaccatgcg cttgccgtcg ggcagccagc aaggcagccg gtaccggatg ccggtagtga
     3541 tcatttcgct gtttaaaggc tcggcatcac tgccatgctg gatgacagcg ccttcccaat
     3601 tggcaaacga gaacagcttg ccgcgcgtgg ttaccgccag agactgtcca ttaggatgca
     3661 gatcccaggc ttcgagatac cgcgccggat ccacgaattt gcggctgcgt tgaacttgcg
     3721 gagagtggaa atcgatcgca attgggtagc tgatatctga ctgcggcgag taaagataga
     3781 gatcagcacc ggcgtggtag acaatctgct ggccatcgct agaaggattg cgtacataat
     3841 aatcatcatg gtctgtatgc cggcgaaggc cagtaccatc tgggaggcat gaataaaggt
     3901 ttccgattcc ttcgtggtca gatataaaat aaatgcgttc tcccagccag atcggatttg
     3961 ccaggttacc tcccagctcg atcaggtgat ggaaatcacc attggcggag gagtcgatcc
     4021 atatttgtcc ggctgtgcct ccccggtagc gtttccagcg ggcgggctcg gttgaaaggc
     4081 ggcccaaaac aacgcctccg tttggaccaa aggagatgtg gcgggccagg ccatagttga
     4141 ttagctcagg tgctccactg gaagggtcga tctggtagag ctgggtaaaa cgctggtacc
     4201 agtgtccggc agtgctggca aatattagtt tgccttcggc tgtccaggct actggctggc
     4261 aagtgagagc gcccatgaat gttaaccggc gggctggacc accttgagct ggcatgagat
     4321 atatttcaga gtgcccctca tcccgaccag caaaagccag tttttgtcca tcggacgaaa
     4381 gcagggggta aatatcttca cttaggctgg cagttaaccg gcgggctatt ccaccgctgg
     4441 caggcacact ccaaaggtca tcctcacaac tgaagacagt ggtctctcct tgaatagtcg
     4501 gaaagcggta ataaccggat gccattttta ctccttggac gaagatttga taaagtgagt
     4561 ttaccatggt tgaaagctgg aagagttgta caggtatact tcttttggat ggtttttggt
     4621 cattccaggt ccaggaaccg gcgtgctata atccaggtca tgaatttttg tcggtatctg
     4681 gcgcatccag accctgatga gagacaagaa tgatcctcgt cacaggtgga acaggttttg
     4741 tcgggaagca gctaattggc caattaacag agttgggtta tccagtccgg acgatggtca
     4801 gcccttcccc gcgctcgcca gatttgccgc gcaatgtacc ggtcgaaatt gctgtaacca
     4861 gtcttacgga tgaacgcgga ttgcgggctg caatggtggg ggttgataca gtctatcacc
     4921 tcatcagcgg cgagtggaaa ggtccacgtg ccagcctgct aaaagtggat attcaaggga
     4981 ccagggcagt tgttgatgct gccaaagatg cgggggtgaa acgtattttt tatctcagcc
     5041 atttgggggc cgatcgagct tcagcttatt cagttttgaa agctaaagga atcgctgagg
     5101 agtttatccg gcggagtggt ttggaataca cgattttccg gactgcgctg gcctatggtc
     5161 agcaggatgc cttcacaacc ggcatcgccg gcatcctcca gacactgcct tacttctttc
     5221 cgattccggg cgatggacgc agcctgttac aacctatctg ggttcaagat ctggttacct
     5281 gcctggcctg ggcgttggac gaccgtggaa cgatcaaccg tttatacgaa attggtggtc
     5341 ctgagtattt cacgttcaaa caagttgtcg agatggtgat ggacaaaatt cagctgcgcc
     5401 ggcgctttct gcatatgcga ccgacttatc tacgctggat gacgattgcc ctcgaagcaa
     5461 tcttcccgaa tctgccggtc tccatatatt ggcttgagta tctggcaaca aaccggacct
     5521 gtgctctgga cactgtaccc cgagagttta cactccttcc tgccaggatg gtacataaga
     5581 ctggttatct acaggaccaa aattggcgct ccattatcct taaatctttg tggcagcgga
     5641 attaaccgga caggattcca gctatgacag gatgggcact tcatattctc gaggaacccg
     5701 aagaaatgga ggcagttgag cggcttcaaa gccaggtatg gccaggcagt gagacggata
     5761 ttgtccctgc gcacctgctc gtaactgcgg ctcacaatgg agggctggta atcggcgcat
     5821 caataaatcc tgatggatca gagcctggcg gcgcaagtga gttagtggga tttgtgtttg
     5881 gttttcctgg tctatataca actcccgatg gtccgcgtcc caaacacgct tcgcatatgc
     5941 tgggcgtccg gcctgatttt cgcaatcgcg gtgttggttt tacactcaag cgagcgcagt
     6001 ggcaaatggt ccgtaatcag ggactggatc tggttacctg gacatttgac cctttattaa
     6061 gtcgcaacgc tcatttgaac attacccagc tcggggcagt gtgtaattat taccggcgga
     6121 atgtatatgg cagtatgcgg gatacggtaa atgccggcct ggactctgac cgcttccagg
     6181 taaactggtg ggtaaattca aagcgcgtcc agcggcggtt a
//
LOCUS       DATMXK010000271         2277 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_121564, whole genome shotgun sequence.
ACCESSION   DATMXK010000271 DATMXK010000000
VERSION     DATMXK010000271.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2277)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2277)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2277
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_121564"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..436
                     /locus_tag="VN363_04975"
     CDS             <1..436
                     /locus_tag="VN363_04975"
                     /inference="COORDINATES: protein motif:HMM:NF018914.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006269 - DNA replication, synthesis of RNA
                     primer [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DnaD domain protein"
                     /protein_id="HWQ83895.1"
                     /translation="LTAETPAAHGSPAEAYYFLNSARGRAAVEAIQRGEWRAPANLPG
                     GPDLSLDHPNIYQLYEENIGPLTPMIAETLREAEQTYPMAWIEEAIRLAVEHNKRSWR
                     YAVAILERWQQEGRDEREDRRDSEAARRRYAEWDAPRKNRRG"
     gene            351..1781
                     /locus_tag="VN363_04980"
     CDS             351..1781
                     /locus_tag="VN363_04980"
                     /inference="COORDINATES: protein motif:HMM:NF013828.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83896.1"
                     /translation="MSEKIDETLRRLAGGMPSGTPPEKTGADKIGDRRYNLLGDPDCP
                     ICHGLGYLRTDLPVVHPEFGRLEICTCRAAQVSLAVRERLFAMSNLNQLSHLTFENFE
                     PRGRMGLWPLEASSLERAFNQSRHFARTLNGWLLLQGPYGCGKTHLAAAVANFAVGIG
                     VPTLFITVPDLLDTLRFAYNDRESSFEERFEEIRNSPLLIMDDFGTQNATPWAQEKLF
                     QLLNYRYINKLPLVVTTNLSLEEIEGRIRSRLEDPDLVTQVRILAQDYRRPASDTGHH
                     ELSSLGLMGRFTFSTFDPREHEDLQADELRSLRSAFDAARAFADKPQGWLVFVGPYGS
                     GKTHLAAAIANFRAEVGYPPLFVVVPDLLDHLRATFSPNSTISMDRRFEEVRTTPLLV
                     LDDLGTQTSSPWAREKLYQLFNYRYNAELPTVITTANFANEMDERLRSRLEDSRLVTV
                     IPITVPGYRGAASPAPRARRGKAKGR"
     gene            1785..>2277
                     /locus_tag="VN363_04985"
     CDS             1785..>2277
                     /locus_tag="VN363_04985"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ83897.1"
                     /translation="MSLMETERLFLRPIETGDLDSLAEIFSDPDVMHYLPAGAPISRD
                     RVHVALARWLDHWKKHGYGFWGVFLKPERRMIGYCGLVHLEDGPEVELAYGFRKPYWG
                     QGLATEAGRACLWYGFQKLGIKRIVGITALGNHASQRVLAKLGLQYEKEAFFYNMICR
                     YFVI"
BASE COUNT          455 a          700 c          681 g          441 t
ORIGIN      
        1 gttgactgct gagacgccag ccgcacatgg cagtcctgcg gaggcatatt actttttaaa
       61 ttctgcccgc ggccgggcgg cggtggaagc catccaacgc ggtgaatggc gggcgcctgc
      121 caacctgccg ggcggtccag atttgagcct ggaccaccct aatatctatc aattgtacga
      181 agaaaacatc ggcccgctga cacccatgat cgctgagact ctgcgtgagg ccgaacaaac
      241 ctacccgatg gcctggatcg aggaagccat ccgcctggca gtggagcaca acaagcgctc
      301 ctggcgctat gcggtggcga tcctggagcg ctggcagcag gaaggacgcg atgagcgaga
      361 agatcgacga gactctgagg cggctcgccg gcggtatgcc gagtgggacg ccccccgaaa
      421 aaaccggcgc ggataaaatc ggcgaccggc gctataacct gctgggcgac ccggactgcc
      481 cgatttgcca cgggttgggc tacctgcgca ccgacctgcc ggttgttcat cccgaatttg
      541 ggcgcctgga gatctgcacc tgcagggcgg ctcaggtgag cctggcggtg cgcgagcggc
      601 tgtttgccat gagcaatctg aaccagctca gccacctgac cttcgagaat tttgagccgc
      661 gcgggcggat gggtctgtgg ccgctggagg cttcctccct ggagcgcgcc ttcaatcaga
      721 gccggcattt tgcgcgcacg ctcaacggct ggctgctgct gcagggtcca tacggctgcg
      781 gtaaaaccca cctggcagcc gcggtcgcaa atttcgcggt tgggattggc gtccccacct
      841 tgttcatcac cgtgcctgat ttgttggaca cactgcgctt cgcatataat gaccgcgagt
      901 ccagctttga agaacgcttc gaggaaatcc gcaattcacc cctgctgatc atggacgatt
      961 tcggcactca aaacgccacg ccctgggcgc aggagaagct cttccagctg ctcaactatc
     1021 gctacatcaa caagctgccc ctggtcgtga ccaccaacct gtccctggaa gagatcgaag
     1081 ggcgcatccg ctcgcgcctg gaagaccccg acctggtcac tcaggtgcgg attctggcgc
     1141 aggattaccg ccgccccgcc agtgacaccg ggcatcatga gctgtcttcg ttaggcttaa
     1201 tggggcgctt tactttctct accttcgacc cccgcgagca cgaagacctc caggcggacg
     1261 aattacgcag cttgcgcagc gccttcgatg cggcgcgcgc atttgccgat aaaccccagg
     1321 gctggctggt tttcgtcggc ccatatggca gcggaaaaac gcacctggct gccgcgatcg
     1381 caaatttccg agcggaggta ggctacccgc ccttgtttgt tgtggttccc gatcttctgg
     1441 accacctgcg ggcaaccttc agccccaaca gcaccatcag catggaccgg cgttttgaag
     1501 aagtccggac cacccccctg ctggtgctgg acgacctggg tacccagacc agcagcccct
     1561 gggcgcgtga gaagctttac cagctcttca attaccgcta caatgccgaa ctgccaacgg
     1621 tgatcaccac cgccaacttc gccaatgaga tggacgagcg gctgcgcagc cgcctggagg
     1681 acagccgtct ggtaaccgtt atcccaatca ccgtgccagg ctaccgcggc gcagcctcgc
     1741 cggcacccag agcccggcgg ggtaaggcta aaggcagatg aaccatgtca ctgatggaga
     1801 ccgagcgcct gtttttacgt cccattgaga cgggcgacct ggacagcctg gcagagattt
     1861 tttctgatcc agatgtgatg cactatctac cagccggcgc gccgatctcc cgcgaccggg
     1921 tgcacgtcgc cctggcccgc tggctggacc actggaaaaa gcacggctat ggattctggg
     1981 gggtgtttct gaaaccagag cggcgcatga tcggctactg cggattggtc cacctcgaag
     2041 acggacctga agtcgagctg gcgtacggct ttcgtaaacc ttattgggga cagggcctgg
     2101 caactgaggc cgggcgggcc tgtttgtggt acggcttcca gaagctgggc atcaagcgga
     2161 ttgtggggat cacagccctg gggaaccatg cctcccagcg agtgctggca aaacttggct
     2221 tgcagtatga gaaagaagct ttcttttaca atatgatctg ccgttatttc gtaatcg
//
LOCUS       DATMXK010000272         3054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_118606, whole genome shotgun sequence.
ACCESSION   DATMXK010000272 DATMXK010000000
VERSION     DATMXK010000272.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118606"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..900
                     /locus_tag="VN363_04990"
     CDS             <1..900
                     /locus_tag="VN363_04990"
                     /EC_number="1.5.1.20"
                     /inference="COORDINATES: protein motif:HMM:NF014296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004489 - methylenetetrahydrofolate
                     reductase (NAD(P)H) activity [Evidence IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA];
                     GO_process: GO:0006555 - methionine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase"
                     /protein_id="HWQ83898.1"
                     /translation="VTAEVSPPLTSAIKKLKSNIDLVKPYVTAVNFTDSPSATPRMSS
                     WACSKICLEQDAEPVMQIAARDRNRTGLQAEVLGASGLGVRNILCLSGDSAKMGPGPR
                     SRMDIVDIDSIQMLWILRRMRDEGKYLDGREMKFPPQYFLGAAAAPFASEPRFQALRE
                     HKKVNAGAQFFQTNVVFDPDGLEIWLNELAKRNLLDKVYILVGITPLKSVRIARHMDE
                     EVPGVYIPQKFIQRLEKAGDKAEEEGVQIALELIEQVKTKQGIHGIHLMAIGWEEIVP
                     RIVREAGLLSREAVINRGEYVKI"
     gene            939..1793
                     /gene="panB"
                     /locus_tag="VN363_04995"
     CDS             939..1793
                     /gene="panB"
                     /locus_tag="VN363_04995"
                     /EC_number="2.1.2.11"
                     /inference="COORDINATES: protein motif:HMM:NF001452.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003864 - 3-methyl-2-oxobutanoate
                     hydroxymethyltransferase activity [Evidence IEA];
                     GO_process: GO:0015940 - pantothenate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-methyl-2-oxobutanoate
                     hydroxymethyltransferase"
                     /protein_id="HWQ83899.1"
                     /translation="MTERKKMTLPMLFKKVADGEPITWLTCYDYPTAYLQEQAGIDMI
                     LVGDSLGMTMLGYESTLPVTMEDMIRHTQAVRRGAPKVFLIGDMPYMSYQPSVETAIR
                     NAGRFMAEAGCDGVKLEGGAEMVDRVRGIVGAGIPIMGHLGLTPQSVSALGGFRVQGK
                     GALQAKKIVDDAKALEEAGVYAILLEMVPDRLCKLITERAEKCFIMSLGSGMDAHGQL
                     LIYHDMFGLYPRFKPRMAKVFGNAGEVILNGLKAYVDEVGNKTFPQPENYFGMDDEAY
                     ADLLKLLE"
     gene            1824..>3054
                     /locus_tag="VN363_05000"
     CDS             1824..>3054
                     /locus_tag="VN363_05000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83900.1"
                     /translation="MDNQISDRLQIPTARLDAINSVLGSEGIRVVEDLLKVVEKYGTP
                     EEINRKAEAARQLPALYARVEAAAPHYLEDLKWLEEQRDQQNFISIQDYRRKLLGTQA
                     DQMEFSPEKAITLEVSAAQYFPFLIHSAQRAIDEETLLPGRFIRVRKMKEQEKDGDLP
                     AFTAAMQIIGASCVETLDTKGTDGSNIHLNGPETITGYFGGIGQPNDYPLKWADELLY
                     YITEYGISQVLNTNSGTVLVGYMLYKLGINFQFKISVFMGNDNPYAGLWTLIGAKLFA
                     REDGSSPLCGLNWSNSINNDTIENSAQFRKQLGFEQVVRFEHHITETYKSIVRQPYNR
                     RDELVEIAPRVANISAKHEGGEPEIDCTREHPSDILDYFRDKEEIIQSGDWANVELNY
                     MDKIDAVNHTAEALTRSG"
BASE COUNT          764 a          757 c          913 g          620 t
ORIGIN      
        1 gttacggcag aagtctcccc gccgctgacc tcagcaatca agaagttgaa aagcaatatt
       61 gatctggtca aaccttacgt aactgcggtc aactttaccg attcgccttc tgccacgccc
      121 cggatgtcct catgggcgtg ctccaagatc tgcctggagc aggatgccga acccgtgatg
      181 cagattgccg cccgcgaccg caaccggacc ggcctgcagg cggaagtttt aggggcgagc
      241 ggattgggtg tgcgcaatat cctgtgcctc tcgggcgaca gcgcgaaaat ggggccggga
      301 ccgcgcagcc ggatggatat cgtggatatt gactcgatcc agatgctgtg gatcctgcgc
      361 cggatgcggg atgaaggcaa atacctggat gggcgggaga tgaaatttcc accccagtat
      421 tttctgggcg cagctgcagc cccatttgcc tctgagccgc gcttccaggc gctgcgcgag
      481 cacaaaaaag tcaacgccgg ggcgcagttc ttccagacca atgtggtctt tgatccggat
      541 gggctggaaa tctggctgaa tgagctggcg aagcgcaacc tgctggataa agtgtatatc
      601 ctggtcggaa tcaccccatt gaagagcgtc aggatcgccc gacatatgga tgaagaagtt
      661 cccggggtct acattccgca gaaatttatc cagcgcctgg aaaaagccgg ggataaggcg
      721 gaagaagaag gtgtgcagat tgccctggag ctgattgagc aggtgaaaac caagcaagga
      781 atccacggca tccacctgat ggcgattggc tgggaggaga tcgtaccgcg catcgtgcgt
      841 gaggcaggtt tactgtccag agaggcagtt attaatcggg gagagtacgt aaagatttaa
      901 ggcaaatcca aacaaaatcg aaaacaagga ggcggagcgt gacagagcgc aagaaaatga
      961 ccttgcccat gttgttcaag aaggtcgctg acggtgagcc gatcacctgg ctgacctgct
     1021 acgactaccc gacggcttac ctgcaggagc aggccgggat tgatatgatc ctggtaggcg
     1081 acagcctggg gatgaccatg ctcggttatg aaagcaccct gccggttacc atggaggata
     1141 tgatccgcca cacccaggcg gtgcggcgag gagccccgaa agtcttcttg attggggata
     1201 tgccgtacat gagctaccag cccagtgttg agaccgctat tcgcaatgcg gggcgcttta
     1261 tggcggaagc cggctgtgat ggggttaagc tcgaaggcgg ggcggagatg gtggatcggg
     1321 tgagaggcat cgtgggtgcg ggcatcccga ttatgggcca tcttggactg accccgcaga
     1381 gtgtcagcgc cctgggtggc tttcgagtcc agggcaaagg cgccctgcag gccaagaaga
     1441 tcgtggacga cgccaaagcg ctcgaagaag ctggtgtgta tgccatcctg ctggaaatgg
     1501 tccctgaccg tttgtgcaaa ctgatcaccg aacgcgctga aaaatgtttc attatgagcc
     1561 ttggttccgg gatggacgcc catgggcagc tgttgatcta ccacgatatg tttggcttat
     1621 acccgcgctt caagccgcgt atggcgaaag ttttcggcaa tgctggggaa gtaatcctga
     1681 atggcttaaa agcgtatgtg gatgaggtcg gaaacaaaac cttcccgcag ccagaaaatt
     1741 acttcgggat ggacgatgag gcgtacgctg atttattgaa acttctggag taaagccggg
     1801 cagaacagcc gaaaggagcg aagatggata atcaaataag cgatcgactg cagatcccaa
     1861 cagctcgcct ggatgcgatc aattccgtcc tggggagtga agggatacgg gtcgttgaag
     1921 acctcttgaa agtagtcgag aaatatggca ctcccgagga gatcaaccgc aaggcggaag
     1981 ctgcccgcca gctgccggcc ctctatgcgc gggtggaagc ggctgcgccg cactatctgg
     2041 aagatcttaa gtggctggaa gaacagcgtg accaacaaaa ttttatcagc atccaggact
     2101 accggagaaa gctgctgggc acccaggctg atcagatgga atttagtccg gagaaagcaa
     2161 tcacactgga ggtcagcgcg gcccagtatt tccctttcct gatccattct gcccagcggg
     2221 cgatcgacga agaaaccctg ctgccggggc gttttatccg ggtccgcaag atgaaggagc
     2281 aggagaaaga cggagatctg ccggcgttta cggctgccat gcagatcatc ggcgcttcct
     2341 gcgtggaaac cctggacacc aagggtacgg acggatcgaa tatccatttg aacggcccag
     2401 aaaccattac cggttatttt ggtggaatag gccaacccaa tgattaccct ctaaaatggg
     2461 cagacgagct gctgtactac atcaccgaat atggcatcag ccaggtgctg aataccaatt
     2521 cgggaactgt gctggtgggg tatatgctct acaagctggg gattaatttt cagttcaaga
     2581 tctcagtctt tatgggcaac gataatccat atgccggctt atggaccctg atcggagcca
     2641 agctattcgc ccgcgaagat ggcagctcgc ccttgtgcgg tttaaactgg agtaattcga
     2701 ttaacaatga tacgatcgaa aattctgccc agttccgcaa acaattggga tttgagcaag
     2761 tcgtgcgttt tgagcaccat atcaccgaga cgtataaatc aattgtgcgc cagccgtata
     2821 accggcgaga cgagctggtc gaaatcgccc cacgggtcgc gaatatctca gccaagcatg
     2881 aaggcggaga gccggagatc gactgcaccc gcgagcaccc ttccgatatt ctggattact
     2941 tccgggacaa agaagaaatc atccaatcgg gggattgggc gaacgtggaa ttgaattata
     3001 tggacaaaat tgatgcggtg aaccacacgg cggaagcttt gacccgcagc ggac
//
LOCUS       DATMXK010000273         5843 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_116079, whole genome shotgun sequence.
ACCESSION   DATMXK010000273 DATMXK010000000
VERSION     DATMXK010000273.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5843)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5843)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5843
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116079"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1736)
                     /locus_tag="VN363_05005"
     CDS             complement(<1..1736)
                     /locus_tag="VN363_05005"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83901.1"
                     /translation="MATISTNQPEEIYAKGFDANITRRLVAFALPYRWQLLLALGAMI
                     IHAAASVAGPYLVKVALDSGIAAGSTAVLTQTVLLYLAAALIQWAATFFRINLMVRVG
                     QAIIYDLRSNLFEHLQKLSLGFFTRYSVGRVITRVINDVGVLREFITWAVLAVARDLL
                     TLIGILLAMLSMNNQLTLLTFTVMPVMIGFTIFFRKRSRDNYRNVRKAISWVNSVLAE
                     NINGVRVVQAFSRQETNYRFFRDEVNHNNLEMNLRAARVAAAFPSSIELLGSVAIGLV
                     VWQGGQAVLGSDQLGLTGLTPGVLVAFVLYIQRFFEPIRDLSRRYDTFQSTMASGERI
                     LGLIDTPVEVADAPDAKPLPEIQGEVCIEDVHFHYSDDPTPVLEAIDLHIQPGETVAL
                     VGKTGAGKSTLVKLISRFHDPTQGRILIDGYDLRTITQLSLRSQMGIVLQDPFLFSGT
                     VRENIAFGRLDAGQSEIEAAARAVGANEFIQALPQGYDTPVEEGGLLLSVGQRQLISF
                     ARALLANPRILILDEATSSVDTQTELIIQKALTRLLSGRTSFVIAHRLSTVVQADRIV
                     VIQDGRIQEQGN"
     gene            complement(1743..3530)
                     /locus_tag="VN363_05010"
     CDS             complement(1743..3530)
                     /locus_tag="VN363_05010"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ83902.1"
                     /translation="MSGTLRKYRFQVLLALLTTLGLTAVSLAIPRIIQQVIDNGLAQG
                     NRGYMLWAALAIAILGLITAGLTYTNQYLSEWVAAHIGYDLRNRLYDQIQYLSFSYHD
                     HSQTGQLISRCIEDVRAIERFTGLGIVEVIRLILLVGGITTLLFIDSPRLAVIALFPL
                     IPLVLITTNFGRKVGAYFLAVDQAIGDLSSRLQENVSGAQVVRAFAREDFEIERFATA
                     NRRLYHARIKVNSEWSKIMPTSNLLVTLGTIAILWFGGRMVIAGEMTIGEVVAFNSYL
                     LLLAAPAQQLTWLVNAAGEAAAGAQRAFEILETIPEIKTPREAIQLPILQGRVNFENV
                     SFNYSGKAAAALADIDLQVKPNQLVALIGPTGSGKTSLVNLIPRFYDASAGTVSVDGI
                     DVRKVDLISLRRQIGIVLQTSLLFSVSIHENIAYGRPDASLDEVIAAAKAAQADEFIR
                     HLPLGYETIVGERGVTLSGGQRQRIAIARAVLMDPRILILDDSTSSVDTQTENLIQQA
                     LDKLMEGRTSFVIAHRLSTVRRADLILVLDHGQIVERGTHKELLRRDGLYRQIYDLQL
                     HDRSQFYEDQSETAPAGTLPAASDRDIGR"
     gene            complement(3559..3981)
                     /locus_tag="VN363_05015"
     CDS             complement(3559..3981)
                     /locus_tag="VN363_05015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83903.1"
                     /translation="MDLFFLDPDEIPLPPEEVRIRDLNAEPFPDGRRVRVILEVDPFQ
                     KRPNLDILITDPAGKPAAIASIIESMTKKIEITMHLRGANHTGQYLVRAELYYRKISE
                     AGTPDPSPPIITQVDLAETFFTIDTQNSEADIIPGKNE"
     gene            complement(4042..4857)
                     /locus_tag="VN363_05020"
     CDS             complement(4042..4857)
                     /locus_tag="VN363_05020"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83904.1"
                     /translation="MTFLPLENITIHEHHDNSRIRPLINRLRSSKILRNPPIVTPYQP
                     GSNHYIVLDGANRTMALRAMGFPHVLAQVVEPDDPGLDLQSWNHVIWEMNSRRFLDNL
                     NSIPGLDLEPVPLGSVQANLYETCNLLLVRLGDGTTYSACTCVDDLTERVEFMNQIVN
                     SYQDSARLDRTNSQDVHTLSQIYPLFCGLVIFPQIRIADILRLAAVGCLLPSGITRVT
                     ISPRALHIDYPLSELESDDPIEAKINRLDRWLKDLLARKGVRYYAEATYLFDE"
     gene            complement(5035..>5843)
                     /locus_tag="VN363_05025"
     CDS             complement(5035..>5843)
                     /locus_tag="VN363_05025"
                     /inference="COORDINATES: protein motif:HMM:TIGR00762.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005504 - fatty acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="DegV family protein"
                     /protein_id="HWQ83905.1"
                     /translation="PQDIVSKYPIRVAPQVLIWDNESYLDGVDIAPDAFYKRLANSKS
                     LPSSAQVSTGSFDSLFKEIRAEGRDILAILVSEKLSGTLGSAIKVKQALGDEPIEIIN
                     SHTVASALALIVLETAKAADQGAGLAECKALAEHLAQNTGVVFAVNTLEFLHRGGRIG
                     GASRFIGSALNIKPILEVTNGRIEPLERIRTRHKSISRMIEIVTDRVGDKQPVQLASL
                     HANAEAEGNQLIIEAKARLGGEVHLVCELSPVVGVHVGPGTVGLAYMAGY"
BASE COUNT         1394 a         1526 c         1579 g         1344 t
ORIGIN      
        1 tgattgcctt gctcctggat ccgaccatcc tggatgacca caattcgatc cgcctgaacg
       61 actgttgaaa gccggtgcgc aatgacaaag gaggttcttc cactcaataa gcgggtgagg
      121 gccttctgga tgattaattc ggtctgggta tccacactgg atgtcgcttc atccagaatc
      181 aggatgcggg gatttgccag cagtgccctg gcaaaggaaa tcagctgccg ctgaccgacg
      241 gagagcagga gaccgccttc ctctacaggt gtatcgtatc cctggggcag agcctggatg
      301 aattcatttg caccaacagc tcttgcagcc gcttcaatct cactctggcc cgcatccaga
      361 cgtccgaaag cgatattttc acgcacagtc ccggagaaca ggaagggatc ttgcagaacg
      421 atccccatct ggctgcgcag gctcaattgg gtgattgtcc gtaaatcgta gccatcgatc
      481 aggatccgac cctgtgttgg atcgtggaag cgagaaatca gctttaccag ggtggatttg
      541 cctgccccgg tcttgcccac cagagccact gtttctccag gctggatgtg cagatcgatt
      601 gcttccagca cgggcgtcgg atcatcgctg taatgaaaat ggacgtcctc aatgcacact
      661 tcgccctgga tctcaggcag tggttttgca tccggcgcgt cggcaacttc gaccggtgta
      721 tcgatcaacc ccaggatccg ctcgccgctg gccatggtgg attgaaaggt atcatagcgc
      781 cggctaagat cccggatcgg ctcgaagaat cgctggatat agagtacaaa agccaccagg
      841 acaccggggg taagaccggt aagacccagc tggtcgctgc ccaatacggc ctgcccgcct
      901 tgccagacca ccaggccaat ggcgactgaa cccagcagct caatggagga tggaaaagcc
      961 gctgcgaccc gggcagcacg caggttcatt tccagattgt tgtggttgac ttcatccctg
     1021 aagaagcggt aattcgtttc ctgccgtgaa aacgcctgga cgacacgcac cccgttgata
     1081 ttttccgcca gaactgaatt gacccagctg attgctttgc gtacattgcg gtaattgtcg
     1141 cgcgagcgtt tccggaaaaa gatggtaaaa ccgatcatca caggcatcac tgtaaaagta
     1201 agcagtgtga gctgattatt catgctcagc atggcgagca agatgccaat cagggtcagc
     1261 aggtcgcggg cgactgccag gactgcccag gtgatgaatt ctcgcagcac cccgacatca
     1321 ttaattaccc gcgtgatcac ccgccctacg ctgtagcgcg taaaaaagcc cagggacagc
     1381 ttctgcagat gctcaaagag attggagcgc agatcgtaga tgatcgcctg cccgacccgc
     1441 accatcaggt tgatcctgaa aaatgtcgct gcccattgga tcagagctgc tgccagataa
     1501 agcagtacgg tttgggttaa tacggctgta gaaccggcgg ctatgcctga gtcgagggcg
     1561 actttcacca ggtaaggccc ggctacggaa gcagccgcgt ggatgatcat cgctcctagg
     1621 gccagcagca attgccagcg atagggcagc gcaaacgcga ccaggcggcg ggtgatattg
     1681 gcgtcgaacc cttttgcgta aatctcttcc ggttggtttg tagaaatagt tgccatctac
     1741 tgttatctgc cgatatcccg gtcgctggcg gctggaagag tacctgccgg agctgtttcg
     1801 gattggtctt cataaaattg gctgcggtcg tgcagctgaa gatcatagat ctgtcgatac
     1861 aagccatcgc ggcggagcaa ctccttatgg gtgccgcgtt ccacgatctg cccatgatcc
     1921 agcaccagga ttagatcggc ccggcggacg gttgagagcc ggtgagcgat cacaaaggaa
     1981 gtgcgtcctt ccatgagttt atccagcgcc tgttggatca aattctcagt ctgcgtgtca
     2041 acactggaag tcgaatcatc caggatcaag atgcgtggat ccatcaacac tgccctggcg
     2101 attgcgatac gctggcgctg tccaccagaa agggtcaccc ctcgctcgcc gacaatcgtt
     2161 tcgtagccca ggggcagatg gcggatgaat tcgtctgcct gggcagcttt ggctgcagca
     2221 attacctcat ccaggctggc atccggacgt ccgtaggcga tattctcatg aatcgatacc
     2281 gaaaagagta gtgaggtctg caggacaatc ccgatctgcc gccgcaggct gattaaatca
     2341 accttgcgga catcgatccc atccacagat acagttcctg cgctggcatc gtaaaaacgg
     2401 gggatcaggt tcaccaggct ggtcttccca gaacctgtcg gaccgatcag ggcaaccagc
     2461 tggttgggct tgacctgtaa gtcaatgtct gccagggccg cggctgcctt cccactgtaa
     2521 ttgaacgaaa cattctcgaa attgacccga ccctgtaata tgggcagctg gattgcctcg
     2581 cggggagttt taatctccgg gatcgtctcc aggatttcaa aagcacgctg cgctcctgcc
     2641 gcggcttcac ccgctgcatt taccagccag gtgagctgct gggcaggtgc agcgagcagc
     2701 agcaaataac tgttaaatgc cacgacttca ccaatggtca tttcccccgc gataaccatt
     2761 cgccccccaa accacaaaat cgcgattgtc cccagggtca ccagcaggtt tgaggtgggc
     2821 atgattttgg accactcgct gttcaccttg atccgggcgt gatacaaccg gcgattggca
     2881 gtggcaaagc gttcgatctc gaaatcttca cgggcaaatg cgcgcacaac ctgggctccg
     2941 ctgacgtttt cctgcaggcg cgaagacaga tcgccaatgg cctgatcgac ggccaggaaa
     3001 tacgccccaa ccttgcgacc aaaattggtc gtgatcagga ccagggggat caaggggaac
     3061 aaagcaatga ccgccaggcg agggctatca atgaaaagga gggtggtgat gccgcctacc
     3121 aacaggatca ggcggatgac ttcaacaatc ccaagcccgg tgaaacgctc aatcgcgcgt
     3181 acatcttcaa tgcagcgact gatcagctgg ccagtttggg aatggtcgtg atagctgaac
     3241 gagagatact ggatctgatc atacaggcgg tttcgtagat catagccaat atgagccgcc
     3301 acccattcgc tcaaatattg gtttgtgtac gtcagccctg cggttatcaa tcccagaatg
     3361 gcaattgcca gggcagccca aagcatgtag ccacggttgc cttgtgccag accattatca
     3421 atgacctgtt ggatgatcct tggaatcgcc agacttacag ccgttaatcc gagagtagta
     3481 agcagtgcaa gcagtacctg gaagcgatat tttcgcaggg taccgctcaa ttttcggagt
     3541 gctcgcataa ggttttattt actcgttctt cccggggatg atatccgcct ccgaattttg
     3601 agtgtcgatt gtaaaaaatg tttcggcgag atcgacttga gtgataatcg gaggggatgg
     3661 gtcaggagtt cccgcttcgc taatttttcg ataatagagc tcagcacgca caagatactg
     3721 cccggtgtgg ttagcgccgc gcaggtgcat ggttatttct atctttttgg tcatcgactc
     3781 aatgatgctg gcgatggctg ctggtttacc cgccgggtcg gttatcaaaa tgtcgagatt
     3841 agggcgcttc tggaatgggt caacttctaa aataactcgc acccgccggc catctggaaa
     3901 aggctctgcg ttcagatctc gaatccggac ttcttccgga ggtaagggta tttcatcagg
     3961 gtcgagaaag aataggtcca tgaggtatcc tgtttgggaa cgaaaagtac gcctgattat
     4021 accccctcag ctacgctgct cttattcgtc gaacaaataa gttgcctcag cgtaatagcg
     4081 gacaccttta cgtgccagga ggtcttttaa ccatcgatcc aggcgattga tttttgcttc
     4141 aatcggatcg tctgattcaa gctcgctgag tggatagtct atatggagcg cgcggggtga
     4201 aattgtcact cgcgtgatcc cgctgggcag taaacagccc actgcagcca ggcgcaatat
     4261 atcagctatc ctgatctggg gaaagatcac caaaccacaa aacaaaggat agatctggct
     4321 cagagtgtgt acatcctgcg agttggtgcg gtcgagccgg gcgctgtcct ggtagctgtt
     4381 gacgatttga ttcatgaatt ctacccgctc tgtcaggtca tcaacgcagg tacaggccga
     4441 ataagttgtg ccatctccca ggcggaccag caataggttg caggtttcat acaggttggc
     4501 ctgaacgctc cccaaaggaa caggctccag atccagacct ggaatggaat tgagattatc
     4561 caggaaccgc cgcgagttca tctcccagat aacatgattc cagctttgga gatccaggcc
     4621 tggatcatcc ggctcgacca cctgagccag gacgtgcgga aatcccatgg cgcgtaaagc
     4681 catagtgcgg tttgcgccgt ctaaaacaat ataatggttt gatcccggtt ggtaaggggt
     4741 aacgattggt ggattacgca atatcttgct ggagcgaagc cggttgatca atgggcggat
     4801 gcggctgtta tcgtgatgct catggatcgt aatgttttca agcgggagaa aggtcaaatg
     4861 gggcagttta tgcacgtcag gcctgttcaa ttgaaaaaag gagaggtgaa ttgtcaatat
     4921 tttaccgaat gttttgtcca gtgacaacag gttggtgaga atcagggagc attcgttggg
     4981 ctaaatcgaa tgctccttga tcaaaatgct aatttaatct ggattaggtg gtaattaata
     5041 ccccgccata tatgccagac ccaccgtgcc tggtccaaca tgaacgccga ccacggggct
     5101 gagctcacac acgagatgga cttctccgcc cagacgtgct tttgcctcaa taataagctg
     5161 gttgccttcc gcttcggcat tggcgtgtaa agacgccagc tggacaggct gcttatcacc
     5221 tactctgtcg gtaacaatct cgatcatccg gcttatggac ttatgccgtg tgcggattcg
     5281 ttcgagtggt tcgatgcgcc cattcgttac ttcaaggatt ggcttgatat tcaaggcgct
     5341 gccaataaag cgagacgccc cgccaattcg ccccccacga tggaggaatt ccagagtgtt
     5401 tacggcaaat acaaccccgg tattttgtgc caggtgctct gcaagggctt tgcattctgc
     5461 caggcctgca ccctggtcag cagccttcgc cgtttccagg acaataagag ccaatgcact
     5521 cgccaccgta tgggaattga taatctcgat gggttcgtct ccaagcgcct gtttaacctt
     5581 gatggctgaa ccaagcgtgc cggataattt ttcagagacc aggatggcca ggatatcgcg
     5641 accttctgca cggatctcct tgaaaagcga gtcaaaactc ccagtagaaa cctgagctga
     5701 ggagggtaga gatttagagt tcgccaaccg tttgtaaaaa gcatctggtg cgatatctac
     5761 tccatcgaga tatgactcat tatcccagat gagcacctga ggtgctaccc gaataggata
     5821 tttagaaaca atatcctgtg gga
//
LOCUS       DATMXK010000274         3403 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_91658, whole genome shotgun sequence.
ACCESSION   DATMXK010000274 DATMXK010000000
VERSION     DATMXK010000274.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3403)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3403)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3403
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_91658"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..449
                     /locus_tag="VN363_05030"
     CDS             <1..449
                     /locus_tag="VN363_05030"
                     /inference="COORDINATES: protein motif:HMM:NF014667.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="SCO family protein"
                     /protein_id="HWQ83906.1"
                     /translation="PYQLSAQSGKAVLMFFGYTNCPDVCPLTLAEYRQIKADLGSQAA
                     RVEFVIVTVDPERDTPERMLSYVGSFDSEFIGLSGSMADLEKVWADYWIYRAKVETGS
                     ASGYAMDHTARMYLIDPAGNLRLTYPYGFEVEKIVADLQHMLREAN"
     gene            454..1002
                     /locus_tag="VN363_05035"
     CDS             454..1002
                     /locus_tag="VN363_05035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83907.1"
                     /translation="MKKPSLLRPYLLRLGVILIVSIVFVLIFNEAVFLLQKEDHDRAP
                     RTIELVIPAGTAGRVAAGEDDPAIPAEMVFVMGDTLLVRNEDSASHQLGPVWVPAGAT
                     ASLAMGEPAKLAYACSFSTTRYLNLDVRQPTTFGTRLTALILAAPTLAALLYIYSLLV
                     FPVGGKPSGKTAEATSINEGDA"
     gene            999..1757
                     /locus_tag="VN363_05040"
     CDS             999..1757
                     /locus_tag="VN363_05040"
                     /inference="COORDINATES: protein motif:HMM:NF014193.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SURF1 family protein"
                     /protein_id="HWQ83908.1"
                     /translation="MILKLFSRSWILTTLLVLVGVGVLVRLGIWQLDRLDARRAFNAR
                     VLAQEAAPMLVLDQGALTEPGLAESLTGMEYRAVTVQGEYDFSHQVALRNQSWGNEWG
                     VHLLTPLRITGSDQSILVDRGWIPAEAFKSGEWSQYAEPGMVSVKGILRASQSKPDYG
                     KKNDPEGTLQAWNFANVERIQNQTPYPLLPVYIQQAPDPAWTAMPYRSLPELELTEGP
                     HMGYALQWFAFSAILGVGYPFFVRKQSGAKHANP"
     gene            1744..>3403
                     /locus_tag="VN363_05045"
     CDS             1744..>3403
                     /locus_tag="VN363_05045"
                     /EC_number="2.5.1.141"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008495 - protoheme IX farnesyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0048034 - heme O biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme o synthase"
                     /protein_id="HWQ83909.1"
                     /translation="MQIRNLLDRLSRNLHHLALAASLVIFLALILGGITRASDAGLAC
                     AGWPLCGGELTPPLDSLGRLAYIHRLLAALGGGLLWMAAGISLLRLRRERLINLPLVS
                     AAILMGVQIVLGRSLALSSSPAAAAFHLGFSLVILGLSTAATTAAYYRAHEEQIPTRL
                     RLGSPFSRLALITLVAVMLMLVSGAVVSASEAAAACPGWPMCGGSLLPADPAGWMNIV
                     HRLLVALAGLLLAGLLIQAWRTQRSQPAVIVAATTAAVLFFSQALLGAQQVAQSFPPA
                     MQALHTATAVAVWAALAVLILVLGIAGRTARDERSEADAIIGRRGLIKDFLLLTKPVV
                     VLLLLVTTYAGMVIGARAWPSLELTFWTLLGGFLAAGGSGAMNQYIDRFDDEKMQRTQ
                     KRPLPSGRLTPGEGLAFGVGIALASFYLLVAFVNLLAALLSLAGIIYYVLLYSILLKK
                     TTVQNIVIGGGAGAIPPLVGWAAATGSLNVPSLLLFAIVFMWTPPHFWALALVRRQDY
                     ARAGVPMLPVVRGEQETRWQILLYTIELVGLTLLLPLFGLGGSIY"
BASE COUNT          573 a          993 c         1055 g          782 t
ORIGIN      
        1 gtccttacca gctctcggcg cagagcggca aagctgtgct gatgttcttt ggttatacca
       61 actgcccgga tgtctgcccg ctgacgctgg ctgaatatcg ccagatcaaa gccgatttag
      121 gctctcaggc tgcccgggtc gagttcgtta tcgtcacggt cgacccggag cgggataccc
      181 cggaacgcat gctgagttat gtgggcagtt ttgattcaga gtttattggt ctgagcggga
      241 gtatggcgga tctggaaaag gtctgggcgg attattggat ctaccgggcg aaagtcgaga
      301 ccggcagtgc cagcggctat gccatggacc atacggcccg gatgtacctg atcgatcctg
      361 ctggaaacct gcgtctgact tacccgtatg gttttgaagt ggaaaaaata gtcgccgatc
      421 ttcagcatat gctgcgagag gcgaattaaa cctatgaaaa agcccagcct gctccgaccc
      481 tatctgctcc gcctgggagt gatcctcatt gtgtcgatcg tctttgtcct gatcttcaat
      541 gaggctgtct ttttgctgca aaaagaagat cacgaccgcg ctccccggac catcgagctg
      601 gtcatcccgg ccggaacagc cggtcgggtg gcagccggtg aagatgaccc ggcaatcccg
      661 gccgaaatgg tgtttgtgat gggtgatacc ctgctggtgc ggaacgagga ctccgccagc
      721 caccagcttg ggcctgtctg ggttccggcg ggggcaaccg ccagcctggc aatgggcgag
      781 ccggcgaaac tggcctatgc gtgctcgttc tcaaccacgc gttatttgaa cctggatgtg
      841 cgccagccga ccacctttgg cacgcgcctg accgcgctca tcctcgctgc gccaacgctg
      901 gctgctttac tgtatatcta cagcctgctg gtcttcccgg tggggggtaa accgtccggg
      961 aaaaccgctg aagctacctc gataaatgag ggcgatgcat gatcttgaag ttgttcagcc
     1021 ggtcgtggat cctgaccacc ctgcttgtac tggtgggtgt gggggttctg gtgcgcctgg
     1081 gaatctggca gctggaccgc ctggatgctc gccgggcctt caacgcccgt gtattggctc
     1141 aggaagcggc gccgatgctg gtcctcgatc agggggctct caccgagcct ggcctggctg
     1201 agagcctcac agggatggaa taccgggctg tgactgtcca gggcgagtac gacttttctc
     1261 atcaggttgc cctgcgcaac cagtcctggg gcaatgagtg gggggtccat ctgctgacgc
     1321 cgctgcggat cacaggcagc gaccagagta tcctggtgga tcgcggttgg atcccggcag
     1381 aggcattcaa atccggggaa tggtcgcagt atgctgagcc gggcatggtc agcgtgaaag
     1441 gtatcctgcg cgcttcgcaa agtaagcccg attatgggaa gaaaaacgat cctgaaggga
     1501 cgctgcaggc ctggaatttc gccaatgtcg agcgcatcca gaatcagaca ccctatccgc
     1561 tgctgccagt ctatatccag caagcccccg acccggcctg gaccgcaatg ccttaccgca
     1621 gcctgccaga gctggagctg accgaaggcc ctcatatggg ctatgctctg cagtggtttg
     1681 ctttttccgc catcctgggg gtaggctacc ccttctttgt tcgtaaacaa tctggagcga
     1741 agcatgcaaa tccgtaatct acttgatcgc ctgagtcgaa atctccacca cctggcgctg
     1801 gcagccagcc tggtgatctt tcttgcgctc atcctgggcg ggatcactcg cgccagtgac
     1861 gccggcttag cctgcgccgg ctggccgctg tgtgggggcg agcttacccc tccgctggac
     1921 agcctgggtc gattggctta tattcaccgt ttattggccg ctttgggcgg cgggctgtta
     1981 tggatggcag cggggatatc cctgctgcgt ctgcgccggg aacgtttgat caacctgccg
     2041 ttggtcagcg cggccatcct gatgggggtc cagattgtcc ttgggcgctc gctggcgcta
     2101 tccagctccc cggccgccgc agcctttcac ctgggtttct cgctcgttat tctggggctg
     2161 tccactgccg ccaccacagc ggcttattac cgtgcccatg aagaacaaat tcccactcgg
     2221 ctccggctgg gcagcccgtt cagccgtctg gcattgatca cgctggtggc tgtcatgctg
     2281 atgctggtca gcggggcggt ggtttcagcc agcgaggctg cggctgcctg cccgggatgg
     2341 ccaatgtgcg gcggctccct gctgccggct gatcccgctg gctggatgaa tatcgtgcac
     2401 cgcctcctgg ttgccctggc aggtctgctc ctggctggat tactgatcca ggcctggcgc
     2461 acccagcgta gccaacctgc ggtgatcgtg gccgccacca ctgccgcagt cctgttcttt
     2521 tctcaagctt tgctgggagc ccagcaggta gcccagtctt tcccacctgc aatgcaggct
     2581 ctgcataccg ctactgcagt tgccgtctgg gcggcgctgg cagttttgat cctggtgttg
     2641 gggatcgccg gacgaactgc ccgggatgaa cgcagtgagg cggatgcgat tattgggcgg
     2701 cgcggcctga tcaaggattt tctgctgctc accaagccgg ttgtggtgct gctcctgctg
     2761 gtcacgactt atgccggcat ggtgatcgga gcgcgagcct ggccctctct cgagctgacc
     2821 ttctggacac tcctgggcgg gttcctcgcc gcaggtggct cgggggcgat gaaccagtac
     2881 atcgaccgct ttgatgacga aaaaatgcag cgcactcaaa agcggcccct gccttcaggg
     2941 cggctgactc ccggcgaagg tctggccttt ggagtaggga ttgcgctggc ttcattttat
     3001 ctgctggttg ctttcgtcaa tctcctggcg gcattattga gcctggcagg tattatctat
     3061 tacgttttgc tctacagcat cctccttaag aagaccaccg tgcaaaatat cgtcattggg
     3121 ggcggcgcag gggcaatccc gccgctggtg ggttgggccg cggcgacagg cagcctcaat
     3181 gtcccatccc tgcttctgtt cgcgattgtc ttcatgtgga ctccgcctca cttctgggct
     3241 ttagcgctgg tccggcgcca ggattacgcc cgggccgggg tgcccatgct gccggtggtg
     3301 cgcggtgagc aggagacccg ctggcagatc ctgctgtata cgatcgagct ggttggtttg
     3361 acgcttttac tgccgctctt tggcctgggt gggagtatct atc
//
LOCUS       DATMXK010000275         5037 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_117057, whole genome shotgun sequence.
ACCESSION   DATMXK010000275 DATMXK010000000
VERSION     DATMXK010000275.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5037)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5037)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5037
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117057"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            308..1150
                     /locus_tag="VN363_05050"
     CDS             308..1150
                     /locus_tag="VN363_05050"
                     /inference="COORDINATES: protein motif:HMM:TIGR01409.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocation signal
                     domain-containing protein"
                     /protein_id="HWQ83910.1"
                     /translation="MTQVQRREKAPEPKPEEPKNEGGGISRREFLGMTGTGVAGLVIG
                     GVLGREVFPKVAPVAEPAAAPAAEPAPAETGAAATPQYMAGFTIVFDPYQCTGCMRCA
                     MACSEKYSAEVAPEATADLINLEFARIRPMRFQYVDVINICHDCKLMPWAEGSEQAPC
                     QEVCPEDAILTVPEGEGKAGYYGMGYKYVDREKCLGEDKCWRCAEICEDQFGSGISFD
                     PIEKKAQVCGRCGGDPECVKACPEPMALTWMPAMRNGRFHAFQPTGYAELLYRKMFNQ
                     RRTL"
     gene            1150..3183
                     /locus_tag="VN363_05055"
     CDS             1150..3183
                     /locus_tag="VN363_05055"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0016625 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, iron-sulfur
                     protein as acceptor [Evidence IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase C-terminal
                     domain-containing protein"
                     /protein_id="HWQ83911.1"
                     /translation="MADLKGMTGKIARVDLTSGTVTVIEPEEALYKKFLGGSALGVYY
                     LIKEGIADPKVDPLGPDNMFQIMIGPTTGAGVNNRSVTITKSAYNFIGISTSGGQNSS
                     ELKYAGWDGIQVVGKAAKPVYIAIIDDKVEIRDAGHVWGKGAEEAEMILKAEVTADLE
                     TRESMVRDADLTAEWAATHPPQRKGFGAKRFAQAWVIGQGGENQVWYASVMTEAGRAH
                     GRYGTGAIMGSKNLKAVVVRGTKGHRVADKKKFREIVEAIQASERGDAFWREYGTAGI
                     GAASAYIENSYPIRNWQWCTWSDPQVRGMTGPFMYNASFVRKQACPGCILHCLYPVEI
                     TSDDELMNGHASDMPDWEAMGMIGGNLGYFEMDGTSPEDTFTGSYADQAEHLAKLQFT
                     TYLHDNYGLDYIEGGANLSLLMELRQRDLITVDDLDGIDLKWGDVHAVDALMKKIITR
                     EGIGDKLANGTWETAKYFAELKNNPEIMKYPVTTHRYGQPAHDVRSGGDKNAMEYLTV
                     HRPTEHTGGGTGAFIAGDYPAAIAGQDNKTASADAMGVCSFPAGHWAGKTIDFIKAAT
                     GWTDFTEEDLNKVGAREFAMARLFDIHTQQLTNPKEEWDSLIPNRWFEPVPNGPGAGN
                     AAFGGDRNKLLNEALPAYWKARGWTEDKGIPTLDTLKALEIDDIVGDLAQQYL"
     gene            3287..3841
                     /locus_tag="VN363_05060"
     CDS             3287..3841
                     /locus_tag="VN363_05060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83912.1"
                     /translation="MVTVGGKRVEINLLGVLGSLLMILAMFFPWWTFAMEFNEPTNLY
                     PYLLDGPASEIIGYKRSPQMTLLTVVLFVCILLALVGAFSPKRRSRLLLVISGVLNSL
                     AVWRLLERVGGVAAIYNMPIQGESIASYGGFAKVKVWSRIQPGLYLDVAGIVFILLGA
                     IFLWSTFIRPENHPVMTEEASADG"
     gene            3930..>5037
                     /locus_tag="VN363_05065"
     CDS             3930..>5037
                     /locus_tag="VN363_05065"
                     /inference="COORDINATES: protein motif:HMM:NF024877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase domain-containing
                     protein"
                     /protein_id="HWQ83913.1"
                     /translation="MAKRKPSLQEKAACEGELSRRDFLRTAVVAGATLGAVNIVSGCS
                     PQTASGSPTATVQDVTGGRYLRMDDNNNRPTMPSDYKPAPTFTPQPEVDWIGRTVSTP
                     TPRGSYTSVWYCGACGKQFVSLDLFLTHAANNHAWRLPEIRQVDKPTYEQFIVQPLER
                     FDERNTVFSRTDLDAEYQTRRNAAPLKNIINPTQALEGQALLAGGIYVDNKAGTLHEK
                     YYGYFAHVRGAGGLYDWDEPVNTTRYPVDDPVAMSTRVKEVARIYGADLVGITRLDQR
                     WVYSDWFERATQKTGKLVVNKKYAIVLAIEMNGSLINQSPGLESSAEVALAYSRMAEV
                     AASLAAYIRALGYAATPHGNDTTQSIPLAIDAGLG"
BASE COUNT         1173 a         1327 c         1435 g         1102 t
ORIGIN      
        1 atcaaaaaat agcccttctg gttatttata atcgcttgcc ttagtattgt gaatgaaaac
       61 caataagact atgatctaag tgggtcacca gacatgacaa actcttcaca ttgcaggtga
      121 tcggtatcgg ctttgattaa taatcgaagg atgatctggg tgatttcttt tgactgagaa
      181 aattacccaa atcaccagaa ttgagattga cgagcagaat aggagggcgc tgaaattcta
      241 ttgatttcag gtaaaatgtt cggttcgata cccatcgaag agggctaaaa agctggagga
      301 ataaaagatg acccaagttc aacgaagaga gaaagcacca gaaccaaagc cggaagaacc
      361 aaaaaatgag gggggtggaa tcagccggcg agaattcctg ggcatgaccg ggaccggcgt
      421 tgccgggtta gtcattggcg gcgttttggg acgcgaagtt ttccccaaag ttgccccggt
      481 ggctgagcca gctgccgcac cggctgccga acccgcgccg gctgaaacgg gagccgctgc
      541 tacaccgcag tatatggctg gcttcacgat cgtgtttgat ccctatcaat gcactggctg
      601 tatgcgctgc gctatggctt gctcggaaaa atactccgct gaagtagcgc cggaagccac
      661 tgccgatctc atcaatctgg agtttgcgcg gatccgacca atgcgcttcc aatatgttga
      721 cgtgatcaat atctgccatg attgcaagct tatgccctgg gcggaaggtt ccgaacaggc
      781 gccgtgccag gaagtctgcc ctgaagatgc catcctgacc gttcccgagg gtgaaggcaa
      841 ggcaggttac tacggcatgg gctataagta cgtcgatcgc gagaagtgcc tgggtgaaga
      901 taaatgctgg cgctgtgccg agatctgcga agatcaattc ggcagcggta tttccttcga
      961 tcctattgag aagaaggctc aggtttgtgg gcgctgcggc ggcgatccgg agtgcgtcaa
     1021 ggcatgccca gagccgatgg ctctgacctg gatgcctgcc atgcgcaacg gccgcttcca
     1081 tgccttccaa ccgacaggct atgccgaatt gctgtaccgc aagatgttca accagaggag
     1141 gaccctataa tggccgactt aaaaggtatg acaggcaaaa ttgcccgagt cgacctgacc
     1201 agtggcactg tcactgttat cgaacctgag gaagctctgt ataagaaatt cctcggcggc
     1261 tcggcgttgg gggtctatta cctcatcaaa gaagggatcg ctgatcccaa ggtagatccc
     1321 ctcggtcccg acaacatgtt tcagatcatg atcggaccga ccaccggtgc gggcgtcaat
     1381 aatcgctctg tcaccatcac caagtctgca tataacttta tcggcatctc tacatctggc
     1441 gggcaaaatt cctccgagct gaaatacgcc ggttgggatg ggattcaggt agtcggcaag
     1501 gcagccaaac cggtctatat cgccatcatc gacgacaaag ttgagatccg cgatgccggg
     1561 cacgtttggg gtaagggcgc tgaagaagca gagatgatcc tgaaagcaga agttaccgcc
     1621 gatcttgaaa cccgcgaatc gatggtccgc gatgctgacc tgaccgccga gtgggcagcc
     1681 actcacccgc cccagcgcaa aggttttggc gccaagcgct tcgcccaggc ctgggtcatt
     1741 ggtcagggcg gcgaaaatca ggtctggtat gccagtgtca tgacggaagc cggccgcgcc
     1801 catgggcgtt acggcaccgg ggcgatcatg ggttcgaaga atctcaaggc tgtcgtcgtc
     1861 cgcggcacca agggccatcg cgtggccgat aagaaaaaat tccgtgagat cgtcgaagcg
     1921 atccaggctt ccgaacgcgg cgatgccttc tggcgtgaat acggtactgc gggcatcggc
     1981 gcggcttctg cctatatcga gaactcctac ccgatccgca actggcagtg gtgcacatgg
     2041 tccgatcctc aggtcagagg tatgaccggc ccattcatgt ataatgccag ctttgtccgc
     2101 aagcaggcct gccctggctg catcctgcac tgcctctacc cggttgagat caccagcgat
     2161 gatgagctga tgaatggaca tgcctccgac atgcctgact gggaagccat gggtatgatc
     2221 ggcggaaacc tgggctattt tgaaatggac ggcacgagcc cagaagacac cttcaccggc
     2281 agctatgctg accaggccga acatcttgcc aagctgcagt tcaccaccta tctgcatgac
     2341 aactatgggc tggattacat cgaaggtggc gccaacctgt ctttactgat ggagctgcgc
     2401 cagcgcgatt tgatcactgt tgacgacctg gatggcattg acctgaaatg gggcgatgtc
     2461 catgccgtcg atgctttgat gaagaagatt atcacccgcg aaggcatcgg cgataagctg
     2521 gccaatggta cctgggaaac tgccaagtac tttgccgagt tgaagaacaa tccagagatc
     2581 atgaagtacc cggttaccac ccatcgttat ggccagcctg cccacgacgt gcgcagcggc
     2641 ggcgataaga acgccatgga gtatctcacc gtccaccgcc ccaccgaaca caccggcggc
     2701 ggcacgggtg cctttatcgc cggagattac cccgcagcca tcgctggaca ggacaacaaa
     2761 accgcttctg cggatgccat gggcgtctgt tcgttccctg ccggtcactg ggcgggtaag
     2821 accattgatt tcatcaaggc tgccaccggg tggactgact tcaccgagga agacctgaat
     2881 aaagtcgggg cgcgcgagtt tgccatggcc cgcctgttcg atatccacac ccagcagctc
     2941 accaatccca aagaagaatg ggacagcctg attccgaacc gctggtttga accggttccg
     3001 aatggacctg gagctggcaa cgcggcattt ggcggcgacc ggaataagct tttgaacgaa
     3061 gccctgccgg cgtactggaa agcccgcggc tggactgaag acaagggcat tcctacactg
     3121 gataccttga aagctttaga gattgacgat atcgttggcg atctcgctca acaatatctc
     3181 taaatcatat acgggtcccg gataatttga agcgacgagg ccccagctgg gtggtttcca
     3241 tcaagttggg gcctcgtctg caacctcaga cggttgaata aaagggatgg ttaccgtggg
     3301 tggtaagcga gttgagatta atctcctggg tgtgttgggt tcgctcctga tgatcctggc
     3361 catgttcttt ccgtggtgga ccttcgctat ggaatttaac gaacctacga atctgtatcc
     3421 atatctgctc gatggtccgg ccagtgaaat tatcggctac aagcgcagtc cacagatgac
     3481 tctcctgacc gttgtcctgt ttgtgtgcat cttgctggct ctggtaggcg cattttctcc
     3541 aaaacggcgc tcacgcctgc tgctcgttat cagcggtgtg ttgaactctc tggctgtgtg
     3601 gaggcttctg gagcgggttg gcggcgtggc tgcgatctat aatatgccga tccagggtga
     3661 gtcgatcgcc agttacgggg gatttgcgaa ggttaaagtc tggtccagaa tccaacccgg
     3721 cttgtatctc gatgttgcgg gaattgtctt catcttgctt ggagctatct ttctctggtc
     3781 aacatttatc cggccagaaa atcatccggt aatgactgaa gaagcttcag cggatgggta
     3841 aaaacagcgc taaattgaat gctggatgca gttccgggcg attgactatc cggcaatgac
     3901 ttctcctgaa tgataaaggg attggtatca tggcgaagag aaaaccatcg ctccaagaga
     3961 aggcagcgtg tgaaggtgag ttgagccggc gcgacttttt gcgcacggcg gttgtggctg
     4021 gcgccaccct gggtgcggtc aatattgtct ccggttgcag cccgcaaacc gcatctggtt
     4081 caccaactgc cacagttcag gatgtgacgg gcggcagata cctgcgtatg gacgacaata
     4141 acaaccgtcc aaccatgcct tccgactata agccagcgcc gacatttaca ccccaacctg
     4201 aggtggactg gatcgggcgg acggtatcta caccgacccc gcgcgggtct tatacctctg
     4261 tctggtactg tggggcctgt gggaagcaat ttgtaagcct ggatttattc ctgacccatg
     4321 ccgcaaataa ccatgcctgg cgtttacccg agatccggca ggtagataag ccgacttatg
     4381 aacaattcat cgtccagccg ttggaacgct ttgatgagcg taacaccgtc ttctcccgca
     4441 ctgatctgga tgctgagtat cagacgcggc gcaatgcagc gcccttgaaa aatatcatta
     4501 acccaactca agctctggag ggccaggctc tgctcgccgg agggatttac gtggacaaca
     4561 aagccggtac gctgcacgaa aaatactacg gctattttgc tcacgtgcgc ggcgctggtg
     4621 ggctgtacga ttgggacgaa ccggtgaaca ccacgcggta tccggtggat gatccggtgg
     4681 caatgagcac ccgggtcaaa gaagttgccc ggatctatgg tgcagacctg gtgggtatta
     4741 ccagactcga tcaacgctgg gtgtattctg actggttcga gcgcgccacg cagaaaaccg
     4801 gcaagctggt ggtcaataag aagtatgcga tcgtcctggc catcgaaatg aatggttcgc
     4861 tgatcaatca atcgccgggg cttgaatcca gcgccgaagt tgcgcttgct tactccagga
     4921 tggcagaggt ggctgcctcc ctggcggcgt atattcgcgc cctgggctat gcggccactc
     4981 cgcacgggaa tgatacaacc caaagcatcc ccctggcgat cgatgccggt ctggggg
//
LOCUS       DATMXK010000276         2164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_85014, whole genome shotgun sequence.
ACCESSION   DATMXK010000276 DATMXK010000000
VERSION     DATMXK010000276.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85014"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..867
                     /locus_tag="VN363_05070"
     CDS             <1..867
                     /locus_tag="VN363_05070"
                     /EC_number="3.4.24.-"
                     /inference="COORDINATES: protein motif:HMM:NF013700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M23 family metallopeptidase"
                     /protein_id="HWQ83914.1"
                     /translation="TRFADLHTVIPTRKRMEVAVYEVQSGDTLFGIAEKFALNPQTIL
                     WGNQFVLGDNPHNLRPGQELNILPVDGTYHRWSAGDGLNGVADFFGVTPEDIINFPGN
                     HLDPNTLGDWSNPNIEAGTWLIIPGGKREFVSWSAPEIPRDNPSVAKVLGPGVCGATV
                     DGAIGAGAFIWPANNQRLSGFDYSPSTNHLGVDVAGKEGEAAYAADNGVVVYAGWNNW
                     GYGNMVVINHGNGWQTLYAHLSTYNVTCGQSVYQTNLIGAIGNTGNSSGAHLHFEMMY
                     NGGKVNPWDYLP"
     gene            complement(855..>2164)
                     /locus_tag="VN363_05075"
     CDS             complement(855..>2164)
                     /locus_tag="VN363_05075"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWQ83915.1"
                     /translation="SPTPAPPTTTPTSSSSPTPPDPWAAVDPGGQAIVFWHSQQYERE
                     QVLLEIIAEFNTSNPWGIEVTTAAHTGQNDLFYAAREALNTTAAPDLVYLFQNQAATL
                     QRFDGLVDLTGLLLSPDWGISSTELAEIPPALLAQDIYPALDGIRLGFPTYRSAEVLY
                     INLDWLHELGYANPPQTPQEFKAIACKASQQPFTRRIGSAASLGYALTIDASRFAAWT
                     FAFGGDLYNSDNNAYTFNSPPAVQAMLFLQDLIASGCAEVIEQRYADQDGFARGSILF
                     TLGTSAGIPFYSRAVDEYARFQWNISAPPHLQAEPHVNIYGPSLSILRSTPEKELAAW
                     LLLKHLSTPAVQARWTAVSQYLPVNSAADGLLTTPFADYPALQSADQLISYAQFEPNL
                     PGYEDVRVLVAEALLDIARGAGVVSTLNSLNAQANTILADQGK"
BASE COUNT          468 a          552 c          698 g          446 t
ORIGIN      
        1 acccgatttg ctgacctgca taccgtgatc cccacccgca aacggatgga agtagcggtc
       61 tatgaggttc aaagcgggga tactttattt gggattgcgg agaaatttgc attaaatcca
      121 cagacaatcc tatggggtaa ccagtttgtc ctgggggata atccccacaa cctgcgccct
      181 ggccaggagc tcaatatttt accggtggat ggcacttacc accgctggtc ggctggcgac
      241 ggtttaaatg gcgtggcgga cttctttggc gttacgccgg aagatattat caatttcccc
      301 ggtaaccacc tggatccgaa taccctgggg gattggagca atccgaatat cgaggctgga
      361 acctggttga tcattccagg cggcaagcgt gagtttgtca gctggagcgc gccagaaata
      421 cctcgcgaca acccgagtgt agccaaagtg ctcggcccgg gagtgtgcgg cgcgaccgtg
      481 gatggggcga tcggggcagg tgcttttatc tggccagcca acaaccagcg cctgagtgga
      541 tttgattaca gtccatccac caaccatctg ggcgtcgatg tcgccggtaa agagggcgag
      601 gcggcatatg ccgccgataa tggggtggtg gtgtacgccg gatggaataa ctgggggtac
      661 ggtaatatgg tggtgatcaa ccacgggaat ggctggcaga ccttatatgc ccacctgagc
      721 acctataatg ttacctgtgg acaatcggtc taccagacca acctgatcgg ggcgattggg
      781 aataccggga attccagtgg ggcgcacctg cacttcgaga tgatgtacaa cgggggaaag
      841 gtcaatccct gggattactt accctgatct gccaggatgg tattggcctg ggcgttgagt
      901 gagttcaggg ttgacacgac accggctccc cgggcgatat ccagcagagc ttcagcaacc
      961 agcacgcgca catcctcata accgggcaga ttcggctcga actgcgcata tgatataagc
     1021 tgatccgcgc tctgcagtgc gggataatcg gcaaatgggg tggttaacag cccgtcagcg
     1081 gcagagttaa cgggtaaata ctggctgact gctgtccagc gcgcctggac tgcgggagtg
     1141 gacaggtgct tgagcagcaa ccaggcggct aattcttttt caggcgtgct gcgcaggatg
     1201 ctcaggctgg gcccataaat gttgacgtgc ggttccgcct ggagatgagg cggggcagaa
     1261 atattccatt ggaagcgggc atattcgtcc acggcgcggg agtagaatgg gatccccgcg
     1321 ctggttccga gggtgaacag gatactgccc cgggcaaatc cgtcctggtc tgcatagcgc
     1381 tgctcgatta cttcagcgca tccgctggcg atcaggtcct ggagaaacag catggcctga
     1441 accgcaggcg ggctgttgaa ggtgtaggcg ttgttatctg agttgtacag gtcgccgcca
     1501 aaagcgaatg tccaggcggc aaaccgggaa gcgtcgatgg tgagggcata tcccaggctc
     1561 gcagccgatc caatccgccg ggtaaagggc tgctggctgg ccttacaggc tatggctttg
     1621 aattcctggg gcgtttgagg tggattggca tagcccagct cgtgcagcca gtccaggtta
     1681 atatacagca cttcggcgga gcggtaagtg ggaaaaccca ggcgaatacc gtccagggcc
     1741 gggtaaatat cctgcgccag taaagcgggt ggaatctcag ccagctccgt agaagatatg
     1801 ccccaatctg ggctcagcag cagaccggtc aggtcaacca gcccatcgaa acgctgcagt
     1861 gtggcagcct gattctggaa gagatatacc agatccgggg cggcggtggt attcagcgcc
     1921 tcgcgggcag cgtaaaagag gtcattttgc ccggtgtgcg cggcggttgt gacctcgatc
     1981 ccccatggat tgctggtatt gaactcggca atgatttcca gcaggacctg ctcacgctcg
     2041 tactgctggc tgtgccagaa aacgatggcc tgcccaccag ggtcaaccgc cgcccaggga
     2101 tctggcggtg tggggctgga ggaagatgtg ggcgtggttg tgggcggagc gggggttgga
     2161 ctgg
//
LOCUS       DATMXK010000277         2005 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_120337, whole genome shotgun sequence.
ACCESSION   DATMXK010000277 DATMXK010000000
VERSION     DATMXK010000277.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2005)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2005)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2005
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120337"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            25..1422
                     /gene="larC"
                     /locus_tag="VN363_05080"
     CDS             25..1422
                     /gene="larC"
                     /locus_tag="VN363_05080"
                     /EC_number="4.99.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nickel pincer cofactor biosynthesis protein
                     LarC"
                     /protein_id="HWQ83916.1"
                     /translation="MKTLYLDCFAGVSGDMFLGTLLDLGLDFQRLGEELQKLPIEGYK
                     LQFQRVEKAGLQAASFKVYLTAPDGKAHLADAEFQEVDHAHHHDHSDHDHEHGQADSK
                     TGPAHHHPDPNSRSLTEILELVDNSQLSEGVKKRVGLVFQRLGAAEARVHGKTLETIH
                     FHEVGGTDAIVDIAGSVIGLDLLGVENLVISPLHLGSGFIRFSHGLFPVPAPATTAMV
                     EGFPVYTTDIKGELVTPTGAALVTALASSFGALPAMTVEKVGYGAGSREREFPNVLRG
                     YLGETSDSVPHFTTPNRDPYPAQHQAPAGPGGYHTSPALVLECNLDDMNPQLFEPLLT
                     ALLAAGALDVLMIPAQMKKNRPGTLLQVLANPNGLEYLLEIIFRNTTTLGVRTYPVSK
                     HMLQRQTTQVDTIAGVVRVKTGWLGNECVNWMPEYEDVLSLAQTTGHPAKEIYQQALA
                     AAQTQFSKIKGEPSG"
     gene            1419..>2005
                     /locus_tag="VN363_05085"
     CDS             1419..>2005
                     /locus_tag="VN363_05085"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_011109796.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWQ83917.1"
                     /translation="MTASPNAWWKTAVFYQIYPRSFMDANGDGVGDLEGIRQKLEYLV
                     QLGIDAIWISPIYPSPMIDFGYDVSDYCGIHPMFGSMEDFDRLLEEAHQRGLKIVLDW
                     VPNHTSDEHAWFIEARQNRENPRRDWYIWKDANPDGSPPNNWESFFAGSAWEWDAATG
                     QYYLHQFMEQQPDLNWRNTEVRQALYATLRFWLDKG"
BASE COUNT          473 a          534 c          549 g          449 t
ORIGIN      
        1 agttctgtta acgaaagcag gctaatgaaa actctctatc tggattgttt tgcaggtgtc
       61 agcggggata tgttcctggg aacgctcctt gatctgggtc tggattttca gcgtctgggg
      121 gaggagttac agaagctccc gattgagggt tacaagctcc aatttcagag agtagagaaa
      181 gccggtttac aggctgccag cttcaaggta tatctgactg cgccagatgg caaagcccac
      241 ctggctgatg cagagtttca ggaagtcgac cacgctcacc accacgatca ttcagatcac
      301 gaccatgaac acgggcaggc agacagcaag accgggcctg cgcaccacca ccctgatcca
      361 aattcacgct ctctaactga gattctggag ttggtcgata acagccagtt atcggaaggt
      421 gtaaagaagc gcgtcgggct cgtcttccag cgcctgggtg cagcggaggc gcgggtccac
      481 ggtaaaacgt tggagacaat ccattttcat gaagttgggg ggactgatgc gattgtggat
      541 atcgccggat ctgtgattgg cctggatttg ttgggggtcg aaaatctggt catctcgcca
      601 ctccacctgg gatccggttt tatccgcttc tcgcacggct tgtttcccgt tcctgccccg
      661 gctacaaccg ccatggtaga aggattccct gtctacacca ctgatatcaa aggcgagctg
      721 gttaccccta ccggagccgc cctggtaacc gccctggcaa gctcttttgg ggcattaccg
      781 gcaatgacag tggaaaaagt gggctatggg gctggcagtc gcgagcgtga atttccgaat
      841 gtgctgcggg gttacctggg agaaacaagc gattctgtcc cccactttac gacaccgaat
      901 cgcgacccct atcccgctca gcaccaggca ccggccggac caggcggtta ccataccagt
      961 ccggcacttg tcctggagtg caacctggat gatatgaatc cccagctctt cgagccgctg
     1021 ttaactgctt tgctggcggc aggtgcttta gatgtcctga tgatcccggc tcagatgaag
     1081 aaaaaccggc cgggtacatt actgcaggta ttagccaatc caaatggcct ggaatacctg
     1141 ctggagatca tttttcgcaa tacgacaacg ctgggcgttc gcacttatcc ggtcagtaaa
     1201 cacatgctcc agcggcagac cacccaggta gatacgatcg ccggtgttgt gcgggtaaaa
     1261 accggatggt tgggcaatga atgtgtaaac tggatgcccg aatacgaaga tgtgctttcc
     1321 ctggcgcaaa caaccggcca tcccgctaag gaaatttacc agcaagccct ggcggcagcc
     1381 cagacacaat tttccaaaat taaaggagaa ccatccggat gacagcctcc cccaatgcat
     1441 ggtggaaaac cgcggttttc taccagatat atccgcgcag ttttatggat gctaatggcg
     1501 atggcgtagg cgatctggaa ggtatccgtc aaaagctgga ataccttgtc cagttgggaa
     1561 tcgatgcgat ctggatctcc ccgatctatc cttccccgat gattgatttt ggatacgacg
     1621 tatcggatta ctgtgggatt catcccatgt tcggcagcat ggaagatttt gaccggctgc
     1681 tggaagaagc ccaccagcga ggccttaaaa tcgtcctgga ttgggttccc aaccacacct
     1741 ccgacgagca tgcctggttt attgaagccc ggcaaaatcg cgagaatccc cgccgtgact
     1801 ggtatatttg gaaagatgcc aacccggatg gcagcccacc gaacaactgg gagtccttct
     1861 ttgccggatc agcctgggaa tgggatgcag caaccggaca gtattacctg caccaattta
     1921 tggagcagca gcctgacctg aactggcgca acaccgaagt ccgccaggct ttgtatgcaa
     1981 cgctgcgctt ctggctggat aaggg
//
LOCUS       DATMXK010000278         5133 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_144993, whole genome shotgun sequence.
ACCESSION   DATMXK010000278 DATMXK010000000
VERSION     DATMXK010000278.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5133)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5133)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5133
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_144993"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            68..451
                     /gene="rpsP"
                     /locus_tag="VN363_05090"
     CDS             68..451
                     /gene="rpsP"
                     /locus_tag="VN363_05090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560010.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S16"
                     /protein_id="HWQ83918.1"
                     /translation="MVRIRLRRVGAKGQPSYRLVAASKEAPRDGRFLEILGFYNPRTE
                     PATIQLKEDRIYDWISKGAQPSESALQVLKSAGLMARYERFKKGEALEALLEEAKSVE
                     QNRNVSSKTRRDITPQKAKKAKVEA"
     gene            485..721
                     /locus_tag="VN363_05095"
     CDS             485..721
                     /locus_tag="VN363_05095"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560009.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="KH domain-containing protein"
                     /protein_id="HWQ83919.1"
                     /translation="MKALVEYIARSLVDDPTAVSVTLERRGSQQVVKLHVAKEDMGRV
                     IGKSGKVANAMRILLRVAAAQEGKRAVMDIVEPL"
     gene            718..1272
                     /gene="rimM"
                     /locus_tag="VN363_05100"
     CDS             718..1272
                     /gene="rimM"
                     /locus_tag="VN363_05100"
                     /inference="COORDINATES: protein motif:HMM:TIGR02273.1"
                     /note="Essential for efficient processing of 16S rRNA;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_process: GO:0006364 - rRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome maturation factor RimM"
                     /protein_id="HWQ83920.1"
                     /translation="MTQRETDQPTGSPQGEPVFLAVGKLGRPHGIRGEVFLILMTDFP
                     ERLQSNVQVYLGEEYEPLVIRSRRENKDALLLKFDGYDTPEAVGELRNKLLFVRADDR
                     PPLPEGEYYHHQIIGLQAVDEQGDLLGRVTDIIFTGSNDVYIVQPESGPEILLPATEE
                     VILAIDLEQGLIRVHLIPGLLRGE"
     gene            1295..2407
                     /gene="dprA"
                     /locus_tag="VN363_05105"
     CDS             1295..2407
                     /gene="dprA"
                     /locus_tag="VN363_05105"
                     /inference="COORDINATES: protein motif:HMM:TIGR00732.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009294 - DNA-mediated transformation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-processing protein DprA"
                     /protein_id="HWQ83921.1"
                     /translation="MTEPRLAYIGFNLVKGIGAARMRTLLDIFGDVDTAWQASGPDLR
                     NAGLSQRIVDNLLQVRAEVDLTKTWERLERLGISVLTWDDPLYPSRLSEIEQPPPVLY
                     IRGKVIDEDSWAVALVGTRNPTAYGRQVAGEIAETLARSGVTVVSGLARGIDAAAHQA
                     CLEAGGRTLAVLGSGVDLVYPPEHRQLAQKISAQGALVSDYAPGTPPDAANFPPRNRI
                     ISGLARAVIVVEAGEKSGALITAAFAADQGREVYAVPGGIYSPLSKGTNRLIQEGARP
                     MLDCEELLSSLKLGPVTEFHAARSALPMDATETHLLELLGNEALHVDEISQRSNLPVE
                     KISAALVMLELKGMVRQMGGMQFMAIRETGDEYRTG"
     gene            2415..3503
                     /locus_tag="VN363_05110"
     CDS             2415..3503
                     /locus_tag="VN363_05110"
                     /inference="COORDINATES: protein motif:HMM:NF012695.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="mannose-1-phosphate guanylyltransferase"
                     /protein_id="HWQ83922.1"
                     /translation="MIDHYYAVIMAGGGGTRLWPLSRRARPKQLLSLFDERSLFQIAV
                     SRLDGVFPYERILVVTVESQAALLRAQVPEIPAENYLIEPLPRGTASVVGLAAIALQA
                     RDPQAVMAILTSDHYMGNEYRFRRLLEAAFEVAQDGYLVTLGIEPTFPATGYGYIQQG
                     ALLGRFQKQEVYKVLRFKEKPTEEAAREMLESGDHAWNSGMFVWRVDSILDEFKSQMP
                     QLVQTLAEIRRAWDTPQRSETIQRLWPEVKSETIDYGIMEGAPKVAVIPAAGLGWSDV
                     GSWDSLFDVLSGDQHGNIVIGGEHIGIDTERSLMYVSQPHRLIVTIGVEDLVVVDTGD
                     VLLVCRKDQAQKVRQVVDQLKESGQNYI"
     gene            3521..>5133
                     /gene="topA"
                     /locus_tag="VN363_05115"
     CDS             3521..>5133
                     /gene="topA"
                     /locus_tag="VN363_05115"
                     /EC_number="5.6.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560005.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003917 - DNA topoisomerase type I (single
                     strand cut, ATP-independent) activity [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I DNA topoisomerase"
                     /protein_id="HWQ83923.1"
                     /translation="MDAYCVKCKQKREIQDPQAVYTGSGTPATRGVCGVCGTTLFRMG
                     RTDAHAALTPPDKEAVRAARRSGKLVIVESPAKARTVGRFLGKGYTVKASVGHVRDLL
                     RSQLSVDVDNGFAPKYRVPNEKRPIVKEIKNLAKSAAEIFLATDPDREGEAIAWHLME
                     AAEIDPKMTQRVVFHEITAPAIAEAFDHPRQINMDLVDAQQTRRILDRLVGYNLSPLL
                     WRKVRSRLSAGRVQSVALRLIVEREREIEAFVPVEYWSIHAEFLPEGLKETFLAKLQR
                     VDNEEPELGSENFVNPIIQDMQQAAYAISRIKRGERRKTPAAPFTTSTLQQDASRRLG
                     YTAKRTMALAQQLYEGIDTGEGGTIGLITYMRTDSTNVSPLAQAEAREFIQQRYGDSF
                     LPAEPPQYKTKVRGAQEAHEAIRPTSVLRQPESLKDHLNRDQLRLYQLIWQRFMASQM
                     APALFDTLAVDVAGKTPQHEYLLRASGSTLRFQGFLIVYEESKDEDRATDEDEDNTRI
                     PAGLVEGQTQQLKRLIPEQHFTQPPPRFS"
BASE COUNT         1200 a         1301 c         1468 g         1164 t
ORIGIN      
        1 acctcaatcc gccgtgcaag tccaatcaca attcaataac ttgtagactt gaaggagaaa
       61 gatttttatg gttcgcattc gtttgcgccg tgttggagcg aagggacaac ccagctaccg
      121 tctggtagcc gccagtaagg aagccccgcg ggatggtcgc tttttagaga ttctcggttt
      181 ctataatcct cgcacagaac cagcgactat ccaattaaaa gaggatcgga tttacgactg
      241 gattagcaag ggcgctcagc ccagcgaatc tgcgttgcag gtgctcaaat cagcaggcct
      301 tatggcccgc tatgagcgtt tcaagaaagg cgaagctctg gaagcattgc tggaagaagc
      361 taagtctgta gaacaaaacc gcaatgtctc cagtaagact cgccgtgata taacgcctca
      421 gaaagctaag aaagccaagg ttgaggcctg atcctggttt atcgttttgt tccggaggta
      481 aagcatgaaa gcccttgtgg aatacatcgc tcggtctctt gtagatgatc cgacggctgt
      541 gtctgtaacc ctggaacggc gtggctcgca gcaggtggtc aaacttcacg ttgccaaaga
      601 agacatgggt cgggtgattg gaaagagcgg aaaagttgcc aatgccatgc ggattttgct
      661 gcgcgtggct gctgctcaag aaggcaaacg ggcagttatg gacattgtag agcccttatg
      721 acccaacgtg aaactgatca accaacaggc tcgccgcagg gcgagcctgt gttcttagcc
      781 gttggcaaat taggccgccc tcatggcatc cgtggcgaag tctttttaat cctgatgact
      841 gatttcccag aacgtttgca atcaaatgtg caggtctatc ttggcgagga gtatgagccg
      901 ctagttatcc gctcgcggcg cgagaataaa gatgccttgc tgttaaaatt tgatggttat
      961 gacacgccag aagccgtggg agagctgcgc aataagctgc ttttcgtgcg cgccgatgat
     1021 cgcccacctt tgcctgaagg cgaatattat caccatcaga ttattggttt gcaggctgta
     1081 gatgagcaag gagacttgct tggcagagta accgatatca tatttactgg ctcaaatgat
     1141 gtctatattg tccagcctga gtcagggccg gaaattcttt tgccggcgac tgaggaagta
     1201 atcctcgcta tcgacttaga acaaggcctt atccgcgtcc atttgatccc tgggctgttg
     1261 cggggagaat aacaacccac cttcggcgct aaaaatgacc gaaccacgac ttgcctacat
     1321 tggattcaac ctggtaaagg gaattggcgc tgccaggatg cgcaccttac tcgatatatt
     1381 tggtgatgtt gacactgcct ggcaggcttc gggaccagat ttacggaatg caggcctgag
     1441 ccaacggatc gtggacaacc tcctgcaggt gcgtgcggaa gttgatctta ccaaaacctg
     1501 ggaacgcctt gaacggctgg ggatttcagt gcttacctgg gatgatccgc tctaccccag
     1561 caggctctcg gaaatcgaac aacctccgcc ggtattgtat atacggggaa aggtcattga
     1621 tgaagacagc tgggctgttg ccctcgtggg cacgcgcaac cccaccgctt acggacgcca
     1681 ggttgcgggc gagatcgccg aaacactggc ccgcagtggc gtgactgtcg tatcaggtct
     1741 ggcgcgtggc atcgatgctg ctgctcacca ggcttgcctg gaggctggcg ggcgaacgct
     1801 ggcagtttta ggcagtggag ttgatctggt ttatccacct gagcaccgcc agttggccca
     1861 gaaaatctct gctcaagggg cattggtgag cgattacgcc ccgggaacgc ctcccgatgc
     1921 agccaatttt ccacctcgca accggattat ctccggtctg gcgcgggctg tcattgtggt
     1981 cgaagccggc gaaaaaagcg gcgccctgat taccgcagcc ttcgctgctg accaggggag
     2041 ggaagtatat gctgtgcctg gcggcatcta ttcaccgctc tctaaaggca caaaccggct
     2101 aatccaggag ggcgcccgtc cgatgctgga ctgtgaggag ctgctttcga gcttaaagtt
     2161 aggtccagta actgagtttc acgccgctcg atccgcattg cccatggatg ctaccgaaac
     2221 acatttgctg gagctgctgg ggaatgaagc actgcatgtg gatgaaatca gccagcgttc
     2281 caatttaccg gtcgagaaaa tatcggctgc actggtcatg ctggaactaa aagggatggt
     2341 acgtcagatg ggcgggatgc agtttatggc tatccgcgaa acaggcgatg agtatcgaac
     2401 agggtaggag aaggatgatc gatcattatt atgccgtcat catggccgga ggtgggggta
     2461 cccgcctgtg gccgctatca cgccgtgcca ggccgaaaca gctcttgagc ttgtttgacg
     2521 agcgctcgct gtttcagatt gctgtaagcc ggctggatgg cgtttttccg tatgaacgta
     2581 tcctggtagt cacagttgaa tcgcaagcgg cgctattacg cgcccaggtt ccagagatcc
     2641 cggcggaaaa ttatttgatt gaaccactgc cgcggggtac agcctctgtg gttgggctgg
     2701 ctgccattgc ccttcaggcg cgcgatcccc aggctgtgat ggcgatcttg acctcggatc
     2761 actatatggg caatgaatac cgcttccgcc gtttactgga agcggccttc gaagttgctc
     2821 aggatggata cctggttact ctgggaattg aaccgacttt tccggccaca ggttatggct
     2881 acattcagca aggtgcgctg cttggacgtt ttcagaagca ggaagtttac aaggtgctgc
     2941 gtttcaaaga gaaaccgact gaagaggctg cccgtgagat gcttgagtcg ggtgatcatg
     3001 cctggaattc gggcatgttt gtctggaggg tagacagcat cctggatgaa ttcaaaagcc
     3061 agatgcctca actcgtccag actctggctg agatcaggcg ggcatgggat acccctcagc
     3121 ggtcggaaac gatccaacgg ctttggccgg aggttaaaag tgagaccatt gattatggca
     3181 tcatggaagg cgctccaaaa gtcgcagtga tcccggctgc aggattgggt tggagcgatg
     3241 tcgggagctg ggattcctta ttcgacgtat tatcaggaga ccagcatgga aatatcgtta
     3301 tcggaggcga gcatatcggg attgatacgg agcgctcgct gatgtatgta agccagcctc
     3361 accgcttgat tgttaccatc ggcgttgaag atctggtggt tgtggatact ggtgatgtct
     3421 tgttggtttg tcgaaaggat caggctcaga aggtgcggca ggttgttgac caattaaaag
     3481 aaagcggtca aaactacatc taatacctgg agaatattcc atggatgctt attgtgtaaa
     3541 atgtaaacag aaacgtgaaa ttcaagatcc acaggcagta tatactggct cgggcacccc
     3601 ggctacacgg ggagtctgcg gtgtgtgtgg gacaactcta tttcggatgg ggcgcacaga
     3661 tgcccatgct gcgcttacgc ctccggataa agaggccgtc cgggcagctc gccggtcagg
     3721 caaactggtg atcgtggaat cgccggccaa agcgcgcacg gtgggacgct ttctgggcaa
     3781 aggctatacc gtgaaggctt cggtcgggca tgtgcgcgat ttgctgcgtt cgcagttatc
     3841 ggtcgatgta gacaatggtt ttgcgcccaa gtatcgggtt cccaatgaaa aacgccccat
     3901 tgtaaaagaa atcaagaatc tggcaaaatc agccgccgag attttcctgg cgaccgaccc
     3961 ggatcgtgaa ggcgaggcta tcgcctggca cctcatggaa gctgccgaga tcgatccaaa
     4021 aatgacccaa cgggtggtct tccatgaaat cacagcccct gctatcgccg aagccttcga
     4081 ccatccgcga cagatcaaca tggatctggt agatgcccaa cagacccggc gaatcctgga
     4141 tcgtctggtt ggctataact tgagtccact gttatggcgt aaagtccgca gccgcctttc
     4201 cgcaggtcgt gtccagtcgg ttgcacttcg cttgattgta gagcgcgagc gcgaaattga
     4261 ggcttttgta cccgtggagt actggtctat ccatgcggaa tttttgcctg aggggctgaa
     4321 agaaaccttc ctggccaagc tgcagcgggt cgataacgaa gagcctgagc tgggcagcga
     4381 aaatttcgtt aacccgatta ttcaggacat gcagcaagct gcgtatgcca tcagccggat
     4441 aaagcgagga gagcggcgca aaaccccggc tgccccattt acgaccagca ccttgcagca
     4501 ggatgcatcc cgccgcctgg gctataccgc aaagcgaacc atggccctgg cccagcagct
     4561 ttacgaaggc attgataccg gcgaaggtgg cacgattggc ctgataacct atatgcggac
     4621 cgactcgacc aacgtttctc ctctggcaca ggctgaagcg cgtgagttca tccagcagcg
     4681 ttatggggat agtttcctgc cagccgagcc cccccagtat aagactaaag tgcgcggcgc
     4741 acaggaagcc catgaagcca tccggccaac ttctgtcttg cgccagcccg aatcgctcaa
     4801 agaccatctt aaccgcgacc agctgcgtct gtatcaatta atctggcagc gtttcatggc
     4861 ttcccagatg gcaccagctt tatttgatac gcttgcggtg gatgtggctg gaaagactcc
     4921 ccaacacgag tacctgctgc gggcttcggg ctcgacgctc cgattccagg gctttttgat
     4981 tgtttatgag gaatctaagg atgaagaccg ggccacggat gaagacgaag acaatactcg
     5041 catcccggca ggcctggtag agggtcaaac tcagcagctg aagaggctca tccccgaaca
     5101 gcactttacc caacctccgc cgcgcttctc aga
//
LOCUS       DATMXK010000279         3086 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_72549, whole genome shotgun sequence.
ACCESSION   DATMXK010000279 DATMXK010000000
VERSION     DATMXK010000279.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3086)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3086)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3086
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72549"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(304..1608)
                     /locus_tag="VN363_05120"
     CDS             complement(304..1608)
                     /locus_tag="VN363_05120"
                     /inference="COORDINATES: protein motif:HMM:NF039823.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5667 domain-containing protein"
                     /protein_id="HWQ83924.1"
                     /translation="MNPNPVPESRLPDPRLIEKISVLNDLPERDPRRTSQGKAAFLAA
                     AQEIAGASALIAVGAPAQRSAGVTPGQKRRHSGWMDAIQSLFVIRRKERSPMFAALST
                     LLIIASLVLTGGGAAVAAAQTSQPDQALYNLKLFSEDMRLNLETDPAADCQLALAFAA
                     RRAAEIQTLLQAGGAPPEPVLLRYQAQVEQAIQFGLNLPHDQAVQALEQVRTSLQGQE
                     QALLQLQANGSPQAEAALLRTRSMVQERLQWVEMGLLDPAQLREQLRMHDQSGDPLQP
                     GPGEPAGGAGASLTCTPAYDGHNSQNPGTTGTPVPGSGYGPGPGDGDCQDGTPAYDGS
                     SSQNPWTTSTPVPGSGYGPGPGDGECADCTPATGTPSAGPGPLQPTPLPPQPTQAGPQ
                     PTQYQQPTQASQPPLASPQPTQKQQPTQAGPQPTQSGPGGRP"
     gene            complement(1586..2161)
                     /locus_tag="VN363_05125"
     CDS             complement(1586..2161)
                     /locus_tag="VN363_05125"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ83925.1"
                     /translation="MGEPSELELLNGARAYDLPTLGDIYDRYSPGIYRYALRLTGDDC
                     LAEDCLADTFMRFLKALQIGQGPRDHLQAYLYRIAHNWITDYYRRQPPPQLNLDETLA
                     GDGADYPEWQAEKHLEQIQVRLAFRSLTPDQRQVIVLRFLDGWENAEVAAALQKPVGA
                     IKALQHRALIMLRKQLLSAEKDGIDEPKPCT"
     gene            2376..2813
                     /locus_tag="VN363_05130"
     CDS             2376..2813
                     /locus_tag="VN363_05130"
                     /inference="COORDINATES: protein motif:HMM:NF012241.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Hsp20/alpha crystallin family protein"
                     /protein_id="HWQ83926.1"
                     /translation="MLVRWNPYREMNHMRRMMDRVYDENLAQRWNEAERSASPLALDV
                     KESDEAYLVKATLPGIDPQDVEITYNNKTLTIQGELKNEEEHENERYHMRERYSGKFY
                     RQLRLPFPINEEAIEAKYENGILTLTLPKSEELKPKKIVVQAV"
BASE COUNT          612 a          848 c          997 g          629 t
ORIGIN      
        1 catcagaact gagctgcgga aagctttgct ctatccatgg ccagaccgta caggttttaa
       61 ggcagtttga atcgaccgga acccggcggg ggattgggaa tccgtccagg cgggtggtat
      121 gcggaaacct gtacggtcgt cattgagcca ccggctgcgc gcagccacgg aagcctggaa
      181 tttcagtctg ttgaagatgg aatgaaaaaa ccgctgcgcg ccgcagccgg aagctactgc
      241 gacgcgcaga cgggtctggg gtatcggcca gcggcaggaa tcaccctgcc gctggcgctc
      301 gttctagggt cttccgccag gtccggattg ggtcggctgt ggaccggctt gagtcggctg
      361 ctgtttctgg gtcggctggg ggctagccag cggaggctgg ctcgcctgag tcggctgctg
      421 gtactgggtc ggctgtggac cggcttgagt cggctgcggc gggagcgggg tcggctggag
      481 aggaccaggt ccggcggacg gcgtgcctgt ggcaggcgtg cagtcggcgc attccccatc
      541 cccggggccg gggccgtagc cgctgcctgg aaccggggta ctggtggtcc atggattctg
      601 gctgctgctg ccatcatagg ccggggtacc gtcctgacag tcgccgtcac ccggaccagg
      661 tccatagccg ctgcctggaa cgggagtacc cgtagtcccg ggattctggc tgttgtggcc
      721 atcataggcc ggggtacagg tcaggctcgc gccagcgccg ccagccggct caccgggacc
      781 aggctgcagc ggatcgcctg actggtcgtg catgcgcagt tgttcacgca attgagccgg
      841 gtcgagcaga cccatttcca cccactgcag gcgttcctgc accatcgacc gggtgcgcag
      901 cagggcggcc tcagcctgcg gtgagccatt cgcctggagc tggagcaggg cttgctcctg
      961 gccttgcagg ctggtccgta cctgctcgag agcctgtacg gcctggtcat ggggaaggtt
     1021 taagccaaac tggatggcct gctccacctg ggcctggtag cgcagcagga caggctccgg
     1081 gggcgcgccg ccggcctgga gtaaggtctg aatttcagca gcgcgccgcg cggcaaaggc
     1141 cagggccagc tggcaatcag cggcgggatc ggtttccagg ttcaggcgca tatcttcgct
     1201 gaataatttc aggttataca gcgcctggtc gggctggctt gtctgagcgg cagccacggc
     1261 ggcacccccg cccgtgagga cgaggctggc aatgatcaac agggtgctca aagctgcaaa
     1321 catgggggat cgctcctttc ggcggatcac aaacagggat tggatggcgt ccatccaccc
     1381 gctatgacgc cgtttttggc caggagttac gcccgccgat ctctgggcgg gtgcgcccac
     1441 cgcgattagt gcagaggcgc ccgcaatttc ctgggcggct gccaggaaag cggctttgcc
     1501 ctggctggtc cgccggggat cgcgctcggg caggtcgttc aggacgctga ttttttcgat
     1561 cagcctggga tcaggtaacc tggattcagg tacagggttt gggttcatct atcccgtctt
     1621 tctcagcgct caaaagctgt ttgcggagca tgatcagggc gcggtgctgg agagctttga
     1681 tggcgcccac cggtttttgc agggcagcgg cgacttcggc gttctcccag ccatccagga
     1741 agcgcaggac gatcacctgg cgctggtcgg gggtcagcga gcgaaaggcc aggcgcacct
     1801 gaatttgctc caggtgcttc tcggcctgcc actccgggta gtcagcgcca tcgccggcca
     1861 gggtctcgtc caggttgagc tgaggtggag gctggcggcg gtagtagtcg gtgatccagt
     1921 tgtgggcgat gcggtaaagg taagcctgga gatggtcgcg cggcccctgc ccaatttgca
     1981 gcgctttaag gaagcgcata aaggtatcgg caaggcagtc ttcagccagg caatcatcgc
     2041 cggtcaggcg cagggcatag cggtagatcc ctgggctgta gcgatcgtag atatcgccca
     2101 gggtgggcag gtcgtatgct cttgcaccgt tcagcagctc cagctcgctc ggttcgccca
     2161 tggctacctg ttatgacgtg aatatgtgct gtgagtgacg gggaaattca taactcgatg
     2221 atagcagacg attttgaaga acttgtgaag aactggcgga agttcttatg cgattctgat
     2281 accgggttta caaccttctt atacaggcag gctatgatta cagcatcaac aataacttta
     2341 ttcgtttcgt aagaaacaag tcaattggag gcaaaatgtt agtccgttgg aacccttacc
     2401 gtgaaatgaa ccacatgcgc cgcatgatgg atcgtgtgta cgacgagaac ctggctcagc
     2461 gctggaacga agccgaacgc tccgcttccc cgctggcact ggatgtgaaa gagagcgatg
     2521 aagcttacct ggtgaaagcc accttacccg ggattgaccc gcaggacgtg gaaatcacct
     2581 acaataacaa gaccctcacc atccagggcg aactgaagaa cgaagaagag cacgaaaacg
     2641 agcgctatca tatgcgtgag cgctacagcg gcaaatttta tcgccagctg cgcttgccct
     2701 tccccatcaa cgaagaagcc atcgaagcca aatacgagaa cggtattttg accctgacac
     2761 tgcccaagag cgaagagctc aagccgaaga aaattgttgt ccaggctgtg taaataattt
     2821 gcagccgcgc tcgatctact gcgcaatata aatgcggatc ttacacagcc ccagcgaaaa
     2881 cctggggctg tgttttgtta tccgggctta gcctcaattc cttagaacct gaagaacttc
     2941 tttcacctta cggtttttgg agtgggatta cccaggcagg caggatgtaa ttgtcgtccg
     3001 gcccgcgctg agcgcccggg ggtaaataat ttcgtaaaga tagtgagagc ttatcgcagg
     3061 tgggcgcggt tggatcggca cacagc
//
LOCUS       DATMXK010000280         6200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_38469, whole genome shotgun sequence.
ACCESSION   DATMXK010000280 DATMXK010000000
VERSION     DATMXK010000280.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6200
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38469"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..799
                     /gene="rplC"
                     /locus_tag="VN363_05135"
     CDS             173..799
                     /gene="rplC"
                     /locus_tag="VN363_05135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L3"
                     /protein_id="HWQ83927.1"
                     /translation="MKGLIGKKVGMTQIFDAAGSAVPVTLIEAGPCYVTQIRTVESEG
                     YAAVQLGFAEAKPKRLTGGQLGHLKRNNLPPLRFLREFREKDPGVAEGDVLKVEVFAV
                     GDYVDVIGTSKGKGFQGGMKRYHFKGGPATHGQSDRQRSPGSRGSTTTPGRVFKGARG
                     PGHMGHDQVTTQNLVIALVDTERNLLGVRGSVPGPKGGLVVIKGARKQ"
     gene            877..1506
                     /gene="rplD"
                     /locus_tag="VN363_05140"
     CDS             877..1506
                     /gene="rplD"
                     /locus_tag="VN363_05140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L4"
                     /protein_id="HWQ83928.1"
                     /translation="MEVDVVNMDGEKVKTLELPAAIFEAPINVDLMHQAYVRQLANAR
                     LGTHKTKTRSEVSGGGRKPWRQKGTGRARQGSIRSAQWVGGGKIHTPRPRDYTLDMPR
                     QMRRAALRSALSVKAAANEIVLVDALALVEGKTKLMAKALDTLVGKSTALVLLPNNGE
                     EYELVLRASSNLPDAKTLLATYLNVRDLLGYDRLIMPVQAVDVLVSYLG"
     gene            1519..1821
                     /gene="rplW"
                     /locus_tag="VN363_05145"
     CDS             1519..1821
                     /gene="rplW"
                     /locus_tag="VN363_05145"
                     /inference="COORDINATES: protein motif:HMM:NF004363.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L23"
                     /protein_id="HWQ83929.1"
                     /translation="MTTIYDVLRRPLVTEKSNYQNSDLHQYVFEVAGSATKSMVKDAV
                     ETLFDVNVLRVNVMHMPAKRTRRAKSRRLMVRRSSFKKAIVTLAPGDTIDVFEGVK"
     gene            1821..2657
                     /gene="rplB"
                     /locus_tag="VN363_05150"
     CDS             1821..2657
                     /gene="rplB"
                     /locus_tag="VN363_05150"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016293504.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L2"
                     /protein_id="HWQ83930.1"
                     /translation="MAVKKYKPTTPGTRGMTGYTFEEITKTTPERSLIRSLRKTGGRN
                     AQGKITVRHHGGGHRQFMRLVDFKREKYGIPARVAAIEYDPNRTARLALLFYADGEKR
                     YIVAPLDLRVDDTVISGPTAEVRPGNNLPISNIPVGTLVHNLELKEGKGGQLVRAAGT
                     AAQLIAKEGDYAQVRMPSGEVRLVRQTCYATIGQVGNLDHSNIKLGKAGRKRHLGIRP
                     TVRGTAMSPRDHPHGGGEGRQPIGMPGPKSPWGKPTLGKKTRHNKETDKYIVRHRGKK
                     RR"
     gene            2730..3017
                     /gene="rpsS"
                     /locus_tag="VN363_05155"
     CDS             2730..3017
                     /gene="rpsS"
                     /locus_tag="VN363_05155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S19"
                     /protein_id="HWQ83931.1"
                     /translation="MGRSLKKGPFISPKLLKKVEAMNDRGEKKVVRTWSRTSTIFPQM
                     VGHTIAVHDGRRHVPIYITENMVGHKLGEFAPTRTFRGHVSEKKTVRKGAV"
     gene            3021..3365
                     /gene="rplV"
                     /locus_tag="VN363_05160"
     CDS             3021..3365
                     /gene="rplV"
                     /locus_tag="VN363_05160"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559434.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L22"
                     /protein_id="HWQ83932.1"
                     /translation="METDIRAFARNVPMSAQKVRLVIDLVRGKDVVQALDMLKFTPNR
                     AAHPVSKVLASAMANGEENFGVSRDDMYVYRVFADEAPTRKWRKFGARGRFKPWLRRT
                     SHITVILREREG"
     gene            3426..4103
                     /gene="rpsC"
                     /locus_tag="VN363_05165"
     CDS             3426..4103
                     /gene="rpsC"
                     /locus_tag="VN363_05165"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559435.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S3"
                     /protein_id="HWQ83933.1"
                     /translation="MGRKVHPIGYRLKINKTWEGRWYAEGEEYVNNLHQDFALRALVR
                     KEADRAGVSRIEIERFPGKAKIVVHTAKPGILIGRKGENVKKLRQSLEAMLGKKIDLE
                     IKEIKSPDLDAYLVARNIADQLERRISYQRAMKRALQQAMRQGGQGIRVEVSGRLSGA
                     EMARTVNMRDGRVPRQTLRADIDYAQSEALTTYGRIGVKVWVYKGEKLAEVEERIEST
                     DGVYVSE"
     gene            4188..4610
                     /gene="rplP"
                     /locus_tag="VN363_05170"
     CDS             4188..4610
                     /gene="rplP"
                     /locus_tag="VN363_05170"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001829782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L16"
                     /protein_id="HWQ83934.1"
                     /translation="MLMPKRVKWRKQMRGRMKGKAQRGAEIAFGDYALQALEPGWVSA
                     RQIEAARRAIVRAMRRRGKFWIRIFPDKPISQKPAETRMGKGKGNVEYWAAVVKPGRI
                     MFEVGGGLPEEISKEALRLAQYKLAIKTRIITREAGGE"
     gene            4610..4822
                     /gene="rpmC"
                     /locus_tag="VN363_05175"
     CDS             4610..4822
                     /gene="rpmC"
                     /locus_tag="VN363_05175"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L29"
                     /protein_id="HWQ83935.1"
                     /translation="MAKQNIAALTTDELNTKLNDARDELMKLRFQQATGELSDTTRLK
                     VTRRLIARLLTELNQRNRTAQMESES"
     gene            4819..5103
                     /gene="rpsQ"
                     /locus_tag="VN363_05180"
     CDS             4819..5103
                     /gene="rpsQ"
                     /locus_tag="VN363_05180"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559438.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S17"
                     /protein_id="HWQ83936.1"
                     /translation="MNQRRRLTGVVVSNKMEKTVVVEISRTYRHRLYKKVVHLRKRLM
                     AHDELGCKPGDNVQVVESQPISKMKKWVVESILRRDEATGEAIVEKEAGA"
     gene            5100..5471
                     /gene="rplN"
                     /locus_tag="VN363_05185"
     CDS             5100..5471
                     /gene="rplN"
                     /locus_tag="VN363_05185"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002460061.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L14"
                     /protein_id="HWQ83937.1"
                     /translation="MIQQESRLKVADNTGARELLVIHVMGGSRRRYGRVGDVVVATVK
                     VATPQGSVKKSEIVKAVIVRCSKEWQREDGSTIRFDDNAAVLLDQDGKNPKGTRIFGP
                     VARELREKGFMKIVSLAPEVL"
     gene            5501..5830
                     /gene="rplX"
                     /locus_tag="VN363_05190"
     CDS             5501..5830
                     /gene="rplX"
                     /locus_tag="VN363_05190"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L24"
                     /protein_id="HWQ83938.1"
                     /translation="MKVKIRKGDTVEVISGRLEDKGKRGEVIKVLPEESRVVVQGVNI
                     RKKHKRQMQTQGRTINPGIVEFEAPLHISNVMLVCPKCNTAVRVSIQRDADKSQRVCK
                     NCEAIID"
     gene            5851..>6200
                     /gene="rplE"
                     /locus_tag="VN363_05195"
     CDS             5851..>6200
                     /gene="rplE"
                     /locus_tag="VN363_05195"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016281718.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L5"
                     /protein_id="HWQ83939.1"
                     /translation="MSHILKDRYTKEVAPALQKALNLENPMEVPRIQKVVVNIGVGEA
                     LDNAKALDAAVGDLTQITGQKPVIMKARKSIANFKLREGRAIGVKVTLRGERMWSFLD
                     RLVNIALPRVRDFRG"
BASE COUNT         1547 a         1486 c         1849 g         1318 t
ORIGIN      
        1 tctgggagag tgcaaagtcc tacgcgttgt gggctgcgta agagcagctg tggtggcaaa
       61 cccctaactg atgctttgcc ccaggctgac tgactgcgct gcgctcaggg atgatgcagc
      121 caagccctgg ctgacagtcc cgaatatatt ttgacaggag aaaaccgaga tgttgaaagg
      181 gctcattggc aagaaagtcg gcatgaccca gattttcgat gctgccggat ctgcggttcc
      241 ggttacgcta atcgaagctg ggccttgcta tgttacccag atccggacag tggaatcaga
      301 aggttatgcc gctgtacagc tgggatttgc ggaagccaag ccgaaacgcc ttaccggtgg
      361 tcaattaggg cacctcaagc gcaataatct tcctccattg cgctttttgc gcgagttccg
      421 ggagaaagat ccgggcgtcg cagaaggcga tgtgctcaaa gttgaggtat ttgcggttgg
      481 cgattatgtc gatgtaattg gcaccagcaa gggcaaaggt tttcagggcg gcatgaagcg
      541 gtaccatttc aagggtggcc cggcgacaca cggtcaatcc gatcgccagc gttcaccagg
      601 ctcacgcggc tctaccacca cccctggccg tgtcttcaag ggtgcacgag gtccgggtca
      661 tatgggccac gatcaggtca ccacgcaaaa tctcgtcatt gcattggtgg atacggagcg
      721 caaccttctg ggtgtgcgcg gttctgtgcc gggtcccaaa ggtggactgg ttgtcatcaa
      781 aggtgcacgg aagcagtaga tgggtgcgaa ctgtccaaac agtccccgcc agatttagcc
      841 cgggctggat ggcagctcaa atggagagaa gctacgatgg aagttgatgt tgtaaatatg
      901 gatggcgaaa aggtcaaaac gttggagctc cctgcggcga tctttgaagc cccaataaat
      961 gtagatttga tgcaccaggc ttatgttcgc cagctggcaa atgcccgcct gggcacccac
     1021 aaaacgaaga ctcgcagtga ggtatcgggc ggcggtcgaa aaccatggcg ccaaaaaggc
     1081 accgggcgtg cacgccaggg ctcgattcga tctgcacaat gggtgggtgg tggaaagatt
     1141 catacccctc ggcctcgcga ttacacattg gatatgcccc gccagatgcg ccgggcggct
     1201 ttacgctctg ctctgagcgt caaagcagcc gcgaatgaga ttgtactggt cgatgccctg
     1261 gcattggttg aaggcaaaac caagctgatg gcaaaagcac tggataccct ggtcggtaaa
     1321 tctactgccc tggtattgct ccccaacaat ggcgaagagt acgagttggt actgcgtgct
     1381 tcaagcaact tgccggatgc aaaaacactc ctggcaactt atctaaatgt tcgcgatctg
     1441 ttgggttacg accggctcat catgcccgtc caggctgtag atgtgcttgt ttcttacttg
     1501 ggataggtag aggtagccat gacgactatt tatgacgtgc ttcgccgccc gctggtgact
     1561 gaaaaatcga actaccagaa tagcgattta caccagtatg tatttgaggt ggccggttct
     1621 gcaactaaaa gcatggttaa agacgctgtt gaaaccttgt ttgatgtaaa tgtgctccgg
     1681 gtgaatgtga tgcacatgcc cgctaaacgc actcggcgtg ccaagagccg ccggttgatg
     1741 gttcgccgca gcagcttcaa aaaagcgatt gttaccctgg caccgggcga taccattgac
     1801 gtgtttgaag gagttaagta atggccgtca agaaatataa accaaccact cctggaactc
     1861 gggggatgac gggttatacc ttcgaagaga tcaccaagac gacccccgag cggtctttga
     1921 tccgctcctt gcgcaagacg ggtgggcgaa atgcccaggg taagataact gtccgccatc
     1981 acggcggcgg acatcgccaa ttcatgcgtc tggtagattt caaacgcgaa aaatatggta
     2041 tcccagcgcg ggtagccgcg atcgagtacg acccgaatcg cacagcacgc ctggcattat
     2101 tgttctatgc cgatggtgag aagcgctaca tcgtcgcacc cttagacctg cgcgttgatg
     2161 atacggttat ttctggaccg actgctgaag tacgccccgg caataacctg ccgatctcca
     2221 atattcccgt tggcaccctg gtgcataatc tggagctgaa agaggggaag ggcggtcagc
     2281 tggtgcgtgc agcgggtaca gctgcgcagc ttattgccaa agaaggtgat tacgcccagg
     2341 tgcgtatgcc ttcgggtgag gttcgtctgg ttcgccagac ctgctacgcc accattggtc
     2401 aggtaggtaa cctggatcat agcaatatca agctgggtaa ggctggacgc aagcgccatc
     2461 tcggtattcg cccgaccgtc cgcggtactg ctatgagccc ccgtgaccat ccgcatggtg
     2521 gcggtgaggg ccgccaaccg attggtatgc ccggacctaa gagcccgtgg ggtaaaccta
     2581 cgctgggcaa gaagacccgc cataacaaag agacggataa atatatcgtc cgtcaccgcg
     2641 ggaagaagcg tcgctaagac agagaagagg gagtacccac ggctgatatt tcagtgtagg
     2701 gctcccagtt gacgagtgga gagtacaaaa tgggacgatc tcttaaaaaa gggcccttta
     2761 tttcgccgaa actacttaag aaggtagagg cgatgaatga ccgtggcgag aaaaaggtag
     2821 tgcgtacttg gagccggacc agcacaatct ttcctcagat ggttgggcac actattgccg
     2881 tgcatgatgg ccgcagacat gtgccgatat acatcactga gaatatggtc gggcacaagt
     2941 tgggcgaatt tgcgccaacg cgtaccttcc gtggccatgt ctctgagaag aaaactgtca
     3001 ggaaaggggc agtgtaagcc atggaaaccg atatccgcgc ttttgctcgc aatgtcccaa
     3061 tgtctgctca gaaagtacgc ctggtcatcg acctggtcag aggcaaggat gttgttcaag
     3121 cgctcgacat gctgaaattc acccccaatc gcgcggctca ccctgtctcc aaggtgctgg
     3181 cttcagcgat ggcgaatggc gaagagaact tcggcgttag ccgggatgat atgtacgtct
     3241 accgtgtttt tgctgatgaa gcgcctaccc ggaaatggcg aaagtttggt gcacgcggcc
     3301 ggttcaaacc ctggttgcgg cgaacctcgc atattacggt gatcctgcgg gagcgggaag
     3361 gatagttttg gtgggcacaa tccatctgcc ggtggcaggt cgggttgagt tcgcaaggag
     3421 atactatggg tcgtaaagta catccaatcg gttatcgtct caagatcaac aagacctggg
     3481 aagggcgctg gtacgccgaa ggcgaagagt acgttaataa cctgcaccag gattttgcac
     3541 tgcgcgcttt agttcgcaag gaagcagacc gcgccggcgt atcccgaatt gaaattgaac
     3601 gcttccccgg gaaagcgaag atcgtggttc acactgcaaa acccggcatc ctgattgggc
     3661 gcaagggcga aaatgtgaaa aaactgcgcc aaagtctgga agcgatgttg ggtaagaaaa
     3721 ttgatctcga gattaaagaa atcaaatcgc cagatctgga tgcttacctg gttgctcgca
     3781 atatcgcaga ccagctggaa cggcggatca gctaccagcg ggcgatgaaa cgagccctcc
     3841 aacaagcaat gcgacagggt gggcagggta ttcgcgtgga agtctcagga cgcttgagtg
     3901 gtgctgaaat ggctcgcacg gtgaatatgc gtgatgggcg agttccgcgc cagactcttc
     3961 gggcggatat tgattatgct caatcggaag ccctgacgac ctatgggcgg atcggtgtga
     4021 aggtctgggt gtataaaggc gaaaaattgg ccgaggtgga agaacggatt gagtccactg
     4081 acggcgttta cgtcagcgaa taaaggtagc ttgccagttg ccatggctca gacaacagtg
     4141 tccggtccag gcagaacagc gagcaagatc gtagcgaggt tgtcattatg ttaatgccaa
     4201 aacgtgtaaa gtggcgaaag caaatgcgcg gccgcatgaa gggcaaggct caacggggtg
     4261 ctgagatcgc ttttggcgat tatgcgctcc aggctttgga acccggttgg gtttcagcac
     4321 gccagattga agccgctcgt cgggcgatcg tgcgtgccat gcgccgccgt ggaaaattct
     4381 ggattcggat cttcccagac aaaccgattt cccaaaaacc tgccgaaacc cgcatgggta
     4441 aagggaaagg taatgtggag tactgggcag cagttgtcaa accaggccgg attatgttcg
     4501 aagtaggtgg tggtttgcct gaggaaattt cgaaagaagc attacggctc gcccagtata
     4561 aattagcgat taaaactcgc attatcaccc gcgaggcagg aggcgaataa tggctaagca
     4621 gaatattgcc gccctgacga cggatgaact gaataccaaa ctgaatgatg cacgcgatga
     4681 gctgatgaag ctgcgtttcc aacaggcgac tggtgaactg agtgacacga cccgcttgaa
     4741 ggtaacccgc cgcttgatcg cccgcctgct gaccgagctg aaccagcgca accggacagc
     4801 tcaaatggag agtgagtcat gaaccagcgc cgccgtctga ctggtgttgt tgttagtaat
     4861 aagatggaaa aaacggttgt ggtagaaatt tcgcgtacct accgccaccg cctgtataag
     4921 aaagttgtcc atctccgcaa gcgcctgatg gcccacgacg agctcggctg caaacccgga
     4981 gacaatgttc aggtagtgga aagccagcca atttcgaaga tgaagaagtg ggttgttgaa
     5041 tccattctgc gtcgcgatga agctacgggt gaggcgattg ttgagaagga ggcgggggca
     5101 tgatccagca ggagtctcgc ctgaaagttg ccgataacac cggcgcccgc gagctgctgg
     5161 tcatccatgt gatgggtggt tcacgccgcc gatacggccg ggttggcgat gtggtagtag
     5221 ctacagtgaa ggttgctacg cctcaaggct cggtgaagaa aagtgaaatt gtgaaggctg
     5281 tcatcgtccg ctgttccaag gaatggcaac gcgaagatgg ctcgaccatc cgctttgacg
     5341 acaatgccgc agttctgctt gaccaagatg gcaagaaccc aaaagggacg cgcatctttg
     5401 gaccggtagc acgtgaactg cgtgaaaaag gttttatgaa gatcgtatcg ctggcgccag
     5461 aagttctcta gcccagtggt agaaggcagg agcgaagacc gtgaaagtga agattcgcaa
     5521 aggtgatacc gttgaggtga tcagcgggcg gctagaagat aaaggtaagc gtggtgaggt
     5581 cattaaggtg ctgcctgagg aaagccgggt ggttgtgcag ggcgtgaata ttcgcaagaa
     5641 gcacaaacgc cagatgcaga ctcagggcag aaccattaat cctggtattg tcgagtttga
     5701 agcaccactg catatctcga atgtcatgct ggtatgcccc aagtgcaata ccgcagtgcg
     5761 ggttagtatc cagcgcgatg cagataaatc gcagcgcgtg tgtaaaaact gtgaagctat
     5821 catcgactga tggccggttg gagttaggac atgagccata tattaaaaga tcgatatacg
     5881 aaagaggtcg cgccagccct gcagaaagcg cttaatctcg agaatccgat ggaagtgccc
     5941 cgtattcaga aagttgtggt caacatcggt gtaggcgagg cattggacaa tgctaaagcg
     6001 ctggatgcag cggtaggtga tctgacccag atcactggcc aaaaacctgt gattatgaaa
     6061 gcacgcaaga gcatcgccaa ttttaagctg cgcgaaggcc gggcgattgg tgtgaaagtt
     6121 actttacgtg gcgagcggat gtggtctttc ctggaccgct tggtgaatat tgccttgccg
     6181 cgtgttcgag acttccgagg
//
LOCUS       DATMXK010000281         6746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_81843, whole genome shotgun sequence.
ACCESSION   DATMXK010000281 DATMXK010000000
VERSION     DATMXK010000281.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6746
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_81843"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..322)
                     /locus_tag="VN363_05200"
     CDS             complement(<1..322)
                     /locus_tag="VN363_05200"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ83940.1"
                     /translation="MLQIQNVSKIYDGGVQALYDVTFDIPKGQFLAVIGLSGSGKSTL
                     LRCINRLVEPTSGTILWNDVDITKASQEELRRIRRKIGMVFQHFNLVHRSSVLTNVLA
                     GRLGY"
     gene            685..2064
                     /locus_tag="VN363_05205"
     CDS             685..2064
                     /locus_tag="VN363_05205"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004326 - tetrahydrofolylpolyglutamate
                     synthase activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008841 - dihydrofolate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="folylpolyglutamate synthase/dihydrofolate
                     synthase family protein"
                     /protein_id="HWQ83941.1"
                     /translation="MDIESRYQQTLEYLYSFVDYSLTHADRYSPEKFDLGRMRVFAEH
                     LGSPHTLYPIIHVAGTKGKGSVSALCAAALSQAGYKVGLYTSPHLQDYNERIQVDGQP
                     ISHATLNELVDELRPYLDAGTELTTFEITTALAFLYFARQGVTAAVFEVGLGGRLDAT
                     NIITPVVSVITSISYDHTQFLGNTLTAIAGEKAGIIKPGVPVVISPQKKEARLALERV
                     AQERGAPLIQVGQDVRYVPEARSLHGQSLLVWPAGEQAQAAEYLEAAEPGAWEPLRLT
                     IPLLGHHQVENAATAYAALRVASEHGLPVSVAAIQSGFATVSWPGRFEILSEHPLVLV
                     DSAHNRDSALKLRLALDDYFPGRPVILIFGASEDKDIDGMFAELMPRIRQVVATRSFH
                     PRAISPEALVEYAHRFGRPASITQSVEEALDLALHLAGEEALVLAAGSIFVAAGVRDT
                     WLKKQTTVE"
     gene            2109..4637
                     /gene="lon"
                     /locus_tag="VN363_05210"
     CDS             2109..4637
                     /gene="lon"
                     /locus_tag="VN363_05210"
                     /EC_number="3.4.21.53"
                     /inference="COORDINATES: protein motif:HMM:TIGR00763.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="endopeptidase La"
                     /protein_id="HWQ83942.1"
                     /translation="MKNNDWYLRDNDEDFGEALHRRTEELYSIPDSQADEDGLIECPV
                     LPLRDLVIFPHMVSPVFLSHEAAILAVEEAQLNDQTVISLTMLDPDVEVPGPHDFLPV
                     GVETAVGRLLSMPDGSSSALVQGRRRVEVVEFIQEEPFLIARARPVYENTIVDRETDA
                     TMRTALEMFQRCVQLDRSLPDEAYLYALNIEDPGWLSDMIISAIAPPLQNRQDLLLAL
                     DPMERLRQVISLLAKEADVLELEDEIHTRAQGEVDRTQREYYLREQMKVIQTELGDSD
                     PWTREVLELRERVEHANLPEEVLPRTLKEIDRLGLMPAMSPEVGIIRTYVEWILDLPW
                     TTTTEDNLDVRHAARVLDRDHFGLPRAKERILEYIAVRSLNPKRQRQPILCFIGPPGT
                     GKTSLGRSIAESLGRKFVRLSLGGVRDEAEIRGHRRTYIGALPGRILQTMRRAGTLNP
                     LFMLDEVDKLGQDFRGDPAAALLEVLDPEQNNSFSDHYLELSYDLSKVMFITTANTTA
                     TIPIALLDRMEVIEFPGYIEEEKLQIANRFLVPRQLEENGLQPEEITISELAILRAIR
                     EYTYEAGVRNLERELGKMCRKIARRKAEGKRYATRITPAYVERFLGPPQFFNQEADRK
                     NEVGVATAVAWTENGGEIMPVEVLLVEGKGNMQITGQIGNVMQESAQAALSYLKSRAR
                     SLEIDPEIFDQVDIHVHIPEGAIPKDGPSAGITMATALTSAFTAREVNCNVGMTGEIT
                     LRGRVLPVGGVREKVLAAHRAGIRTVLLPKRNMKDLIELPKRAREEMNIVPVEHMDQV
                     LQVALVSQEKKTPAARPRRKATRTHVPEAVVQPPAEPAVRNPAV"
     gene            4710..6221
                     /locus_tag="VN363_05215"
     CDS             4710..6221
                     /locus_tag="VN363_05215"
                     /inference="COORDINATES: protein motif:HMM:NF016232.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83943.1"
                     /translation="MTLAQDLQAYLAAGQSPQVHWFPEDVPAARLAGVLVGMANGSGG
                     IVLLGISPRAGRIQGLSDPPSIIDRIFQAALLVDPPLVLPLPQVRYTDDGPVVTVQVP
                     MGLPYVYSLEGRYLIRDGAQTNPLPARRLRQLLLERGAVQFEAQSVPGASLADLDLDK
                     AAAYAAALGVPQDHGVPQDRGVPQDRGLPGDHGVPGERGERGERGVPPGDSAAQILLR
                     RGCLNQETSPNGKSILRPNYAGLLLFGRNPQRWLPNATILAARFTGAAFADHYLKQDI
                     GGTLVDQLHQVEAFFRDNLPSAVRMVGLAHEETLEYPPEAVRELLVNAVAHRDYNQQG
                     DNIHLNIFADRLEVQSPGGLPGPVNLGNLLEARFSRNAVIVQVLADLGFVERLGYGLD
                     RVVRVMRQAGLRQPKFEELGGTFRVTLFSESLAPYAVQLKQVEAYRYLNLNPRQEQAL
                     AYLIQSQRITSRDYQELCPEVHPETLRRDLADLVERDVLIKVGDKRATYYILK"
     gene            6196..>6746
                     /locus_tag="VN363_05220"
     CDS             6196..>6746
                     /locus_tag="VN363_05220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="arginine deiminase family protein"
                     /protein_id="HWQ83944.1"
                     /translation="MPPTTSSNSAAWAAVTHRPSPLISSCQLTFIDRQAIDLRRANAQ
                     HQAYCASLQAAGIQVQVLETSLDLPDSAFVEDCAVILDELAVLTHPGGPSRQPEVERL
                     APFIARIRPVVQVQSPAALEGGDVLRIGKTLLVGESTRTNLAGIAALKEFVQPYGYRV
                     IPVPVRGSLHLKTACTALDDETLL"
BASE COUNT         1331 a         2048 c         2041 g         1326 t
ORIGIN      
        1 cataacccaa ccgccccgcc aggacgttgg tcagaacgct tgagcggtgg accaggttga
       61 aatgctggaa taccatcccg atttttcgcc ggatccggcg cagctcttcc tgggaggctt
      121 tggtgatatc aacatcattc cacagaatgg tgcctgaggt gggttcgacc aggcggttaa
      181 tgcagcgcag cagggtggac ttacccgagc cgctcagccc gatgaccgcc aggaactgtc
      241 ctttcggaat gtcgaaagtg acatcatata gcgcctgaac accgccatca tagattttgg
      301 atacgttttg aatttgcagc ataatgactc ttcaattatc taaagatatg ggggataccc
      361 ggacaccttt gtaagttgtt ttggttacct gataatagcc catacttggc gggctggcaa
      421 ggataatcag ctttcaattt gctgtcatcc cgccggagca attgcctgga acgaagttgg
      481 gctgtcagaa aagaatggca aagctgtccg ctccagccaa tatagcgcgt ttcgcgtcct
      541 gtattcgatc cccccggtaa atgaaaaagc acccaatcga ccagaccggt tggttatcga
      601 tccgggtcag gatgcgggaa ttttcaagag ctgcgtaatc ctcctcttgt ccagcaccta
      661 cgccgcggtt acaatcttgg agcaatggac attgaatccc gctatcagca aaccctcgaa
      721 tatttataca gctttgtcga ttacagcctc acccacgccg accgctactc tcccgagaaa
      781 tttgatctgg ggcggatgcg ggtttttgca gagcacctgg gcagccccca cacactgtat
      841 cccattatcc acgtggcagg caccaaaggc aaaggctcgg tttcggcgct gtgtgcggcg
      901 gcgctgagcc aggcaggtta taaggtcggg ttatatacct cgccccacct gcaagattat
      961 aatgagcgca tccaggtaga tggtcagcct atctcgcacg caactctgaa cgagctggtc
     1021 gacgagctgc gtccttacct ggacgccggc accgagctga caacttttga aattaccacc
     1081 gcattggcgt tcctgtattt tgcccgccag ggcgtcaccg ccgccgtatt cgaagtgggt
     1141 ctgggggggc ggttggatgc caccaatatc atcacgccgg tcgtctcggt cattacttcg
     1201 atctcatatg atcataccca gttcctggga aacacactga cggcgatcgc cggcgagaaa
     1261 gccgggatca tcaaacccgg cgtgccggtg gtaatctcgc ctcagaagaa agaagctcgc
     1321 ctggcgctgg aacgggtggc gcaggagcgc ggcgcgcctt tgatccaggt agggcaggat
     1381 gtgcgctatg tgccagaagc gcgctcgctc catgggcaaa gcctgctggt ctggcctgcc
     1441 ggtgagcagg cccaggctgc tgaatacctg gaggctgccg agccaggcgc ctgggagccc
     1501 ctgcgcctga ccatccccct gttgggtcac caccaggttg agaatgcggc tactgcttat
     1561 gccgcgctgc gcgtggcgag cgagcacggc cttcccgtca gcgtggccgc tatccagagc
     1621 ggttttgcca ccgtgagctg gcccgggcgt ttcgagatcc tctctgagca ccccctggta
     1681 ctggtagact cagcccacaa ccgcgattct gctttgaagc tgcgcctggc gctggacgat
     1741 tactttccgg gccggccggt gatcctcatc tttggagctt cggaagataa ggatatcgac
     1801 gggatgttcg ccgagctgat gccgcgtata cgccaggttg tggcgacccg ttccttccat
     1861 ccccgcgcca tctcaccgga ggcgctggta gaatacgctc atcgctttgg tcgtcccgcc
     1921 agcatcaccc agagcgtgga agaagccctg gatctcgccc tgcacctggc tggcgaggag
     1981 gcgctcgtcc tggcagccgg aagtatcttc gtcgctgcag gtgtgcggga tacctggctg
     2041 aagaaacaaa ctactgttga atagaagatt ggcctgctca aaggctggtc cggataaagg
     2101 aaagcttgat gaaaaataat gactggtatt tacgcgataa tgatgaagat ttcggcgaag
     2161 cgctgcatcg ccgcacagag gagctgtaca gcatccccga ttcgcaggca gatgaagatg
     2221 gcctgatcga atgcccggtg ctgccgctgc gcgacctggt gatttttccc catatggtct
     2281 cgccggtctt cctctcacac gaagcagcca tcctggcggt cgaagaagcc cagctcaacg
     2341 accagactgt catttccctg accatgctcg acccggatgt ggaagtgccc ggtccgcatg
     2401 atttcctgcc ggtgggagtt gaaactgccg tcgggcgctt gctgagcatg cccgatggct
     2461 cgagctctgc cctggtgcag ggccggcggc gggtcgaagt ggtggagttc atccaggaag
     2521 agccgttcct gatcgcccgc gcccgcccgg tatatgaaaa tacaatcgtg gaccgtgaaa
     2581 cggatgccac catgcgcaca gccctggaga tgttccagcg ctgtgtgcag ctcgatcgct
     2641 ccctgccgga tgaagcttac ctgtacgcct taaatatcga agacccgggc tggctgtccg
     2701 atatgatcat ctcggcgatc gcgccgcccc tgcagaaccg ccaggatttg ctcctggccc
     2761 tcgatcccat ggaacgcttg cgccaggtga tcagcctgct ggcaaaggaa gccgatgttc
     2821 tcgagctgga agacgagatc cacacccgcg cccagggcga ggtggaccgc acccagcgtg
     2881 agtattacct gcgcgagcag atgaaggtca tccagaccga gctgggggac agtgacccct
     2941 ggacgcgcga ggtgctggag ctgcgcgagc gggtagagca tgccaacctg ccggaggaag
     3001 tcctgccgcg aactttgaag gagatcgacc gcctggggct gatgccggcg atgtctcctg
     3061 aggtggggat tatccgcact tatgtggaat ggatcctcga tctgccctgg accaccacta
     3121 cagaagataa cctcgacgtg cgccatgccg cccgggtgct ggaccgggat cacttcggtc
     3181 tgccgcgcgc caaggaacgc atcctggaat atattgctgt gcgcagcctg aacccgaaac
     3241 gccagcgcca gccgatcctg tgcttcatcg gcccacccgg cacaggcaag acttctctgg
     3301 ggcggtcgat cgccgagtcc ctggggcgta agtttgtgcg cctttcgttg ggcggcgtgc
     3361 gcgatgaggc agaaattcgc ggccaccgcc ggacgtatat cggagcccta ccgggacgca
     3421 tcctgcaaac catgcgccgg gcggggaccc tgaacccctt gttcatgctg gacgaggtcg
     3481 ataagctggg ccaggatttc cgcggcgacc cggcggcggc cctgctggag gtgctcgacc
     3541 cggagcagaa caattccttc tcagatcatt accttgagct gtcttacgat ctgtccaagg
     3601 tcatgttcat caccactgcc aacaccaccg ccaccatccc gatcgccctg ctggatcgca
     3661 tggaagtcat tgaatttccc ggctatattg aagaagaaaa gctccagatc gccaatcgct
     3721 ttctggtgcc gcgccagctg gaggagaatg gcctgcagcc ggaagagatt accatcagcg
     3781 aactggcgat cctgcgtgcc atccgggaat acacttatga agccggggtc cgcaatttag
     3841 agcgcgagct gggcaaaatg tgccgcaaga tcgcccggcg caaggctgaa ggcaagcgct
     3901 atgccacccg gatcacccct gcgtatgtgg agcgcttcct tggcccgccc cagttcttca
     3961 accaggaagc cgaccgcaag aacgaagtcg gtgtggcgac agccgtcgcc tggacggaga
     4021 acggcgggga gattatgccc gtggaagtgc tcctggtaga gggcaaaggc aacatgcaga
     4081 tcaccgggca gattggaaat gtgatgcagg agtcggcgca agctgctttg tcctatctga
     4141 aatcccgcgc ccgcagcctg gagattgacc cggagatctt tgaccaggtg gatatccatg
     4201 tccacatccc cgagggtgcc atcccgaaag atggccccag tgccgggatt accatggcaa
     4261 ccgccctgac ctcagccttt acagcgcgcg aagtgaactg caacgtcggg atgaccggcg
     4321 agatcaccct gcgcgggcgg gtgctgcccg tgggcggggt gcgcgaaaag gtgctggccg
     4381 cgcaccgcgc cggcatccgc acggtgctgc ttcccaagcg caatatgaaa gacctgatcg
     4441 aattacccaa acgcgctcgg gaggagatga acatcgtccc tgtggagcat atggaccagg
     4501 tcttgcaggt tgccctcgtt tcccaggaga agaagacccc tgctgcgcgt ccccggcgaa
     4561 aagccacccg tactcatgtg ccggaagctg tggtccaacc gcctgcagaa ccggcagtta
     4621 gaaacccggc cgtttagtcg ccttataccg gcgcagctcg ccaccggctt gagctgcgcc
     4681 ggttttctgc cgcgtggagt agccgtccaa tgacccttgc tcaagattta caggcctatc
     4741 tggctgccgg tcagagtccc caggtgcact ggttccctga ggatgtaccc gcggcacgcc
     4801 tggcaggggt gctggtaggg atggcgaacg gcagcggggg tatcgtcctg ctgggaattt
     4861 caccccgcgc cgggcgcatc cagggtctgt cggatccacc gtccataata gaccggattt
     4921 ttcaggcagc cctgctggtc gatccacccc tggttttacc acttccgcag gtgcgttaca
     4981 cagatgatgg gccggttgtg actgtgcagg tcccgatggg tctgccatac gtttacagcc
     5041 tggaaggccg ctacctgatc cgggacggtg cccaaaccaa tcctctgccg gcgcgccgct
     5101 tgcgccagct gctgctcgag cgcggggcag tgcagtttga agcccaatct gtgccgggcg
     5161 cgagcctggc agacctggat ctggataaag ccgccgccta tgcagctgcc ctgggcgtgc
     5221 cgcaggatca tggcgtgccg caggatcgcg gcgtgcctca ggatcgcggc ttgccgggcg
     5281 atcacggcgt accgggcgag cgcggtgagc gcggtgagcg cggcgtgcca cccggggata
     5341 gcgcggcaca gatcctcctg cggcggggct gcctgaacca ggagaccagc cccaatggga
     5401 aatctattct gcgtcccaat tatgccggct tgcttttgtt cgggcgcaat ccccagcgct
     5461 ggctgcccaa tgccaccatc ctggcggcgc gctttaccgg ggcagctttt gccgatcatt
     5521 acctgaagca agatatcggg ggaaccctgg tggaccagct gcaccaggtg gaggctttct
     5581 tccgcgacaa tttgcccagc gcggtccgta tggtgggcct ggcgcatgaa gaaaccctgg
     5641 agtatccgcc tgaggcggtg cgcgagctgc tggtaaatgc cgttgcccat cgcgattaca
     5701 accagcaggg cgataatata cacctgaata tctttgccga ccgcctggaa gtccagtctc
     5761 cgggcggtct gcccggaccg gtaaatctgg gcaatttgct agaagcgcgc ttctcgcgca
     5821 acgcggtgat cgtccaggtg ctggctgacc tgggttttgt ggagcgcctg ggctacgggc
     5881 tggatcgggt ggtgcgggtg atgcgccagg ccgggctgcg ccagccgaaa ttcgaagagc
     5941 tgggcggcac tttccgggtg accctgttta gcgagagcct ggcgccttac gccgtgcagc
     6001 tcaagcaggt ggaggcttac cgctatctga acttaaatcc tcgccaggaa caggctctgg
     6061 cttacctcat ccagagccag cgcattacca gccgcgacta tcaggagctc tgccctgaag
     6121 ttcaccccga gacgctgcgg cgcgacctgg cggacctggt cgagcgggat gtgctgatca
     6181 aggtaggaga taagcgtgcc acctactaca tcctcaaata gcgcagcgtg ggcggctgtt
     6241 acccaccgcc catcgccgct gatcagcagc tgccagctga cctttatcga ccgccaagca
     6301 atcgacctgc ggcgagctaa cgcccaacac caggcctact gcgccagctt acaggccgcc
     6361 gggatccagg tgcaggtgct cgaaaccagc ctggacctgc ccgacagcgc ttttgtggaa
     6421 gactgcgccg tgatcctgga tgagctggcg gtgctcaccc accccggcgg cccatcccgc
     6481 cagccggaag tagaacgcct ggcacctttt atcgcccgga tacgcccggt ggtccaggtc
     6541 caatccccgg cagccctgga aggtggggat gtgctccgga ttggcaaaac cctgctggtg
     6601 ggtgaatcta cccgcaccaa cctggccggt attgcagccc tcaaggaatt tgtccagccg
     6661 tatggctacc gggtgatccc ggtccccgtg cgcggcagcc tgcacctgaa gacagcctgc
     6721 accgccctgg acgatgaaac cctgct
//
LOCUS       DATMXK010000282         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_130212, whole genome shotgun sequence.
ACCESSION   DATMXK010000282 DATMXK010000000
VERSION     DATMXK010000282.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130212"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            51..2072
                     /locus_tag="VN363_05225"
     CDS             51..2072
                     /locus_tag="VN363_05225"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC-F family ATP-binding cassette
                     domain-containing protein"
                     /protein_id="HWQ83945.1"
                     /translation="MSLITASDLGKSFGPVDIFGGISLSIPRDARIAIVGPNGIGKTT
                     LLRILLGIEEPTSGNVTRARNLKLGYLPQEAALDSRHTLWDECLNALADLRAMESELR
                     ELEHQMSSPDLAEDALIRYGKLQVTFENLGGYTYETRIRQILTGLGFETTDFQRPITQ
                     LSGGQRTRAVLARLLLSGPDILILDEPTNHLDIRAVEWLEGYLSQWAGAVLIVSHDRY
                     FLDRVVDHIWEMRRDGLETYRGNYTAYIQQRQERWELLQKIYISEKERLEKELDYIKR
                     NIAGQNTTQAKGKLRRLSREIEAIQNLGFAAVQNKTWAEISSLTDISEHSMGVEEVER
                     RIHSLRPPVKRPPHLHLNLKANLRSGDLVLRTRNLAIGYPDEDRPLFTAPDLLLKRGE
                     CAAIIGPNGAGKTTFLKTLLNKMPPLAGEVILGASLKIGYFAQAHEGLQSDNTLVKEI
                     DIVAPQMLLGEIRDYLARFMFSGEDVFKRVSMLSGGERGRLALAKLSLAQANLLLLDE
                     PTNHLDIPAQEVLQEVLDEFQGTILLVSHDRYLIDALGSQIWEIEPDQAELKVFAGTY
                     SEYKALQGAEKENVPAHQAVAVRQNRTSQQGTGNEERRLRNRIKELEETIHQLEVDMK
                     ALAQQLENPPSEPAKVQELGEKYVHIQAALESQLEEWGKLGENLQNAEA"
BASE COUNT          579 a          580 c          586 g          467 t
ORIGIN      
        1 cagcgataca gccggaagcg gcgcgattat aacacggtat aatctgctct atgtccctca
       61 tcactgccag cgacctgggg aagtccttcg gccccgtaga tatttttggc gggatcagct
      121 tatctattcc cagagatgct cggatcgcaa ttgtcggtcc gaatggcatc ggaaaaacca
      181 ccttgctacg catcctgctc gggatagaag agcccaccag cggaaatgtt acccgcgccc
      241 gtaatctgaa gctcggatat ctgccccagg aagctgctct ggacagccgc cacactttat
      301 gggatgaatg cctgaacgcg ttggcagact tacgcgccat ggaatcggag ctgcgagaac
      361 tagaacatca aatgagcagc ccggacctgg ccgaggacgc tctgatacga tatggtaagc
      421 tccaggttac tttcgagaac ctgggtggct acacctacga aacccgcatt cgccagatcc
      481 tcaccgggct gggctttgaa acaaccgatt tccaacgccc aataacccag ctttcgggtg
      541 gacagcgcac gcgcgctgtt ttagcccggt tattactctc cggtcctgac atcttaatcc
      601 tggatgagcc taccaatcac ctggacatcc gcgcagttga atggttggag ggatatctga
      661 gccagtgggc tggggcggta ttaattgttt cccatgaccg ttatttcctg gatcgggttg
      721 tggatcatat ctgggaaatg cgcagagatg gtctcgaaac ttaccgcggc aattacactg
      781 cctacatcca gcagcgccag gagcgctggg aattactgca aaagatatac atctccgaga
      841 aagaacggct cgaaaaagag ctggattata tcaaacgcaa catcgccgga caaaatacaa
      901 cccaggctaa aggtaagctg cgccgcctga gccgcgagat cgaagcgatc cagaacctgg
      961 gatttgcggc ggtgcaaaat aaaacctggg ccgaaatcag ctctctgaca gacatctctg
     1021 aacactctat gggtgtggaa gaggtcgagc ggcggatcca cagtctgcgc ccccctgtga
     1081 agcgccctcc acacctgcac ttaaatctga aagcgaatct gcgttccggg gatttggttt
     1141 tgcgtacacg aaatctggcg attggctacc cagacgaaga ccgcccattg tttacagcac
     1201 ctgatttatt gcttaaacgc ggtgaatgcg cagcaattat cggcccaaac ggggcaggta
     1261 agactacatt tctcaaaacc ttgctgaaca aaatgccacc acttgcgggt gaggtgatcc
     1321 tgggggcgag cctcaagatc ggttattttg ctcaggctca cgaagggctg caatcagaca
     1381 acaccctggt caaggagatc gatattgttg ccccccagat gctgttgggc gagattcgcg
     1441 actatctggc gcgcttcatg ttctccggag aagatgtatt taagcgagta agcatgcttt
     1501 caggcggcga gcgtggtcga ctggcgctgg ctaaattatc cctcgcgcag gccaacttgt
     1561 tgctgttgga tgaaccaacc aatcaccttg atattcccgc ccaggaagta ttacaggaag
     1621 tgttggacga atttcaaggc acaatcctgt tggtgtccca cgatcgctac ctgatcgatg
     1681 cccttggcag ccagatctgg gaaattgagc cagaccaggc tgagctaaaa gtctttgctg
     1741 ggacgtatag tgagtataag gctttacagg gtgcagagaa ggaaaacgta cctgctcatc
     1801 aagccgttgc cgtccgtcag aatcggacga gccaacaagg tacaggaaac gaagaacgac
     1861 gtttaagaaa tcgcataaaa gagttggaag agactatcca ccagctagaa gttgatatga
     1921 aggccctggc ccagcagctg gagaatcccc cttcagaacc agcgaaagtg caagaattag
     1981 gtgagaaata tgttcacatc caggctgcac tggaatcgca gcttgaagaa tggggaaaac
     2041 tgggagagaa tttgcaaaac gccgaggctt aatcggcatt cgccatggcc tgtacaggct
     2101 cgtcgccatt atagcgacgc aataatggcc agcgctggct gatccagctt accgcgataa
     2161 cacaaccgac cccaccagta atcacagcca gtggcgcacc aaacaattgg gc
//
LOCUS       DATMXK010000283         3862 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_62500, whole genome shotgun sequence.
ACCESSION   DATMXK010000283 DATMXK010000000
VERSION     DATMXK010000283.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3862)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3862)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3862
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62500"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(214..1470)
                     /gene="fabF"
                     /locus_tag="VN363_05230"
     CDS             complement(214..1470)
                     /gene="fabF"
                     /locus_tag="VN363_05230"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWQ83946.1"
                     /translation="MSAQSHSYEPIVITGLGTVNPLGLTAAQSWQAALEGASGVGPIT
                     LFDPQDYQVRIAGEVKGFIPENYVAAKESHRRDRFELFAAAAVQEALAQSGIQLTDTN
                     RERAAVIISSAVGGIQSFSNALDTLRIDGPRRISPFTIPMLMINGASGMTAIDLGIQG
                     PSYSLASACASGADGIGLGWTLLRAGAVDIAFAGASEAPVTPLGIAAFDRVGAISRRL
                     GGDDPAPRPFDRDRDGLVLAEGAAVLVMERLSHARQRGAVILAELAGYAATSDAYHIT
                     APAEDGSGGARAIRDALRSAGISPDEVSYINAHGTGTVLNDLSETRAIKTALGSNAYK
                     IPVSSTKSMTGHMMGATGALEALFCVKAIQESCIPPTINYHTPDPECDLDIVPNQARQ
                     MPVEVAVSNAFGFGGHNAVLVLRKFK"
     gene            complement(1522..2631)
                     /locus_tag="VN363_05235"
     CDS             complement(1522..2631)
                     /locus_tag="VN363_05235"
                     /inference="COORDINATES: protein motif:HMM:NF012512.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWQ83947.1"
                     /translation="MPAIRCTNCGSPYPAEGLPYRCPVCTGIFDFSEQPEFDPALLRE
                     TTTQPGIWRFHPLLGLPEDAGEVTLGEGNTPLVWGEIDKRGVAFKLETLNPTGSFKDR
                     GSAVLASWLKVRGVEEAVEDSSGNAGASFAAYAARAGIRSRVFVPESASGPKRMQIGM
                     YGAEIIAVPGPRSNAARTVQVAAENGAVYASHAYLPQLLPGYATLAYELYEQLSGAPG
                     TVIVPAGQGNLLLAVGRGFELLQRAGLVATLPRLVGVQARTCAPLWAVYQYGISAYGW
                     VTEGETLAEGVRVSRPMRGDAVIRIVEQSQGTFMVVDEEQIISGRDHLAHQGFYVEPT
                     SALVWSAVQQLPPDTPEPVVAILTGSGLKTPSHLV"
     gene            complement(2636..3346)
                     /locus_tag="VN363_05240"
     CDS             complement(2636..3346)
                     /locus_tag="VN363_05240"
                     /inference="COORDINATES: protein motif:HMM:NF014448.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor biosynthesis protein MoaE"
                     /protein_id="HWQ83948.1"
                     /translation="MNKIKVLFFATLKEYTGTRQLELVVPADADIRLVKDHLAAVYPS
                     VKPLLETCIVSINHEFALDDEQLPSEAEIAFFPPVSGGSEKSFPTIYKITEDVLDLDE
                     LVAQVTLPSTGAACVFTGMVRGVTSRGDAHQTSYLEYEAYVPMAEAKMAQVAEEIRER
                     WPVVEGIAIVQRIGRLDPGTPTVLIACSAAHRDTGVFDAARYGIDRLKEIVPVWKKEV
                     GPDGEAWVEGKYLPKAGE"
     gene            complement(3343..3834)
                     /gene="moaC"
                     /locus_tag="VN363_05245"
     CDS             complement(3343..3834)
                     /gene="moaC"
                     /locus_tag="VN363_05245"
                     /EC_number="4.6.1.17"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0006777 - Mo-molybdopterin cofactor
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic pyranopterin monophosphate synthase MoaC"
                     /protein_id="HWQ83949.1"
                     /translation="MTEKLTHLDDQGRAHMVDVGAKPDTQRTAIAGGEIKMLLETFQL
                     IQAGAMKKGDVLTTAQLAGVLAAKKTADLIPLCHPLSLTYIDVRLQLDETLPGVRITA
                     EVRTTGKTGVEMEALTAVSVAALTVYDMAKAVEKTMQIQNIRLLEKHGGMSGDVVNWE
                     VQR"
BASE COUNT          862 a         1127 c         1035 g          838 t
ORIGIN      
        1 ggttaatgcc cggcgcagca aggcgggatc tgtaaacaca acctcggtcc ggcgagtgaa
       61 ttcctcggcg acatccacgc cagggactcc ctgggtatgg ttcctcataa tttcctcctg
      121 gaactcaggg agcgccagaa accccaccaa atggtcgagt atctggcgtt ccatcagttc
      181 aactagaatt tactgtaaag aaatattaca cgctcatttg aatttacgca agactaacac
      241 agcattatga cccccaaaac caaaagcatt actgacagca acctccaccg gcatctggcg
      301 agcctggttg ggaacaatat ccaggtcaca ctcagggtcg ggagtatggt aattaattgt
      361 gggtggaata cagctttcct ggatggcctt cacacagaac agcgcctcca gggcgccggt
      421 agcacccatc atgtggccgg tcatcgattt cgtcgatgag acgggtatct tatatgcatt
      481 ggatcccagg gctgtcttga tggcgcgagt ttctgacaga tcattcaaaa ccgtcccggt
      541 tccgtgagcg ttgatgtagc tgacctcatc aggggagata ccggcagacc ggagagcatc
      601 ccggatcgct cgtgccccac cgctgccatc ttccgccggg gctgtgatgt ggtaggcatc
      661 ggaagtcgca gcatagcccg cgagctctgc cagaattact gctccgcgct gtctggcatg
      721 cgaaagccgc tccatgacga gaactgccgc gccttcggcc agcaccaaac catcccggtc
      781 gcgatcaaat gggcgaggtg caggatcatc ccctcccaga cggcgcgaga tcgctcccac
      841 gcgatcaaac gctgctatac ccaaaggtgt aacaggcgct tcgctggctc ccgcaaaggc
      901 tatatcgacc gcccctgccc gtaaaagcgt ccatcccaat ccaatcccat ctgctccgga
      961 tgcgcaggct gaggcaagcg aataagatgg accctggatc cccagatcaa ttgcggtcat
     1021 tcccgaagcc ccatttatca tcagcatagg gatcgtaaaa ggactgatcc gccgcgggcc
     1081 atcaatccgc agtgtatcga gggcattact gaatgattgt atgccaccga cagcagagga
     1141 aataattacg gctgcgcgct cacggttagt gtcagtaagc tgaatcccgg actgcgccag
     1201 ggcttcttgc actgccgcgg cggcaaataa ctcaaaccgg tcgcgccggt gggattcttt
     1261 tgcggctaca taattttcag ggataaatcc tttgacttca ccagcgatcc gaacctgata
     1321 atcttgcgga tcaaagaggg tgatcggacc aactcccgag gctccctcca gggcagcctg
     1381 ccaggattgg gccgcagtca atcccagggg attgactgtg cccagaccgg tgatgacgat
     1441 tggttcataa gaatgagatt gtgcggacat ccagcctcca ccagtgaaag accggaacat
     1501 acagcttatc acctgtctcg ttcagaccag gtgtgaaggt gttttcaagc cgcttccggt
     1561 caggattgcc accactggct ctggcgtatc gggcggcagc tgctggacag cactccacac
     1621 cagagctgag gtgggctcta cataaaaccc ctggtgagcc agatggtcgc ggccagagat
     1681 gatctgctct tcatcgacta ccatgaaggt cccttgagac tgctctacga tccgaatcac
     1741 ggcatctccg cgcatcggac gggatacccg cacaccctct gccagggtct caccttccgt
     1801 gacccaacca taagcagata tgccgtactg gtagactgcc cataatggtg cgcaagttcg
     1861 agcctgcacg ccgaccagac ggggtaaggt tgcaaccaga ccagcccgct gcaaaagctc
     1921 aaagccgcgt cccactgcca gaagcagatt gccctgcccg gcaggaacaa tcactgttcc
     1981 cggcgcgccg ctcagttgtt cataaagctc gtaagccagg gtggcataac ccggcagaag
     2041 ctgaggcagg taggcgtggc tggcatacac agccccattt tcggcagcca cctgcacggt
     2101 ccgggcggca ttcgaacgcg gaccggggac tgctatgatc tcagctccgt acatgccaat
     2161 ctgcatgcgt tttggaccag aggctgattc agggacaaag acccggctgc gaattcctgc
     2221 ccgggcggca taagcagcaa atgacgctcc ggcattcccc gatgaatctt ccacagcttc
     2281 ttctactccc cggactttca gccagctggc aaggactgcc gagccgcgat ccttgaaaga
     2341 gccggttgga ttgagtgttt cgagcttgaa ggcaacaccc cgtttatcga tttcgcccca
     2401 caccaatggc gtattccctt cccccagggt gacctcaccg gcatcttcag gtaatccgag
     2461 cagcgggtgg aagcgccaga tgcctggttg ggtggtcgtt tccctcaata gggcaggatc
     2521 aaattcaggc tgctctgaaa aatcaaaaat cccggtgcag accgggcagc gataaggcaa
     2581 accttcggca gggtacgggc tgccgcagtt ggtacagcgg attgccggca tgcttttatt
     2641 cccccgcttt tggaagatac ttcccctcga cccaggcttc tccgtctgga ccaacttctt
     2701 ttttccagac cggaacgatc tccttgagcc ggtcaatgcc atagcgggcc gcgtcgaaaa
     2761 ccccggtatc ccggtgagca gccgagcaag cgatcagcac cgttggtgtg ccgggatcaa
     2821 gtctgccaat ccgttggaca atcgcgatcc cttccacaac cggccagcgc tcccggatct
     2881 cctcggcgac ctgagccatc ttggcctcag ccatcgggac ataggcttca tattcaaggt
     2941 aagatgtctg gtgcgcatcg ccgcgcgagg tcactccccg caccattcct gtgaagacgc
     3001 aggcagcccc ggtgctgggc aaagtgacct gggcgaccag ctcatccagg tcgagcacgt
     3061 cttcggtgat tttgtagatt gtaggaaaag atttctctga accgccactg accggtggga
     3121 aaaatgctat ttcggcttcg gaaggcagct gttcatcgtc taaggcaaat tcgtgattga
     3181 tcgagacaat acaggtttca agcagaggtt tgaccgatgg ataaactgcc gccaggtgat
     3241 ctttgaccag gcggatatct gcatcggctg gaacgacaag ctcgagctgg cgggttccgg
     3301 tatattcctt cagggtggca aaaaacaaca ctttgatttt attcatcgct ggacttccca
     3361 attgacaaca tctccactca tcccgccatg cttttcgagc aaacggatat tttgaatctg
     3421 catcgttttt tcaacagctt tcgccatatc gtaaaccgtt aatgctgcca ccgagactgc
     3481 cgtcagggct tccatttcaa cgcctgtctt ccccgttgtg cgtacttcgg cggtgatacg
     3541 cactccgggc agcgtttcat ccagctgcag gcggacatcg atataggtaa gtgatagtgg
     3601 atggcagagg gggatcaggt cggctgtctt tttggctgcc agcacacctg ccagctgagc
     3661 cgtggtgagc acatcaccct ttttcatggc tccagcctgg attagctgaa atgtctccag
     3721 gagcattttt atctcaccgc cagcgatcgc ggtgcgttgt gtgtctggct tggcgccgac
     3781 atcgaccata tgggcgcggc cctggtcatc gaggtgggta agtttttctg tcataggggg
     3841 ctattatagc atttcacgcg at
//
LOCUS       DATMXK010000284         2170 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_112869, whole genome shotgun sequence.
ACCESSION   DATMXK010000284 DATMXK010000000
VERSION     DATMXK010000284.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2170)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2170)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2170
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112869"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            261..1025
                     /locus_tag="VN363_05250"
     CDS             261..1025
                     /locus_tag="VN363_05250"
                     /inference="COORDINATES: protein motif:HMM:NF015827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF333 domain-containing protein"
                     /protein_id="HWQ83950.1"
                     /translation="MSRIIRITIYYMLLLVLAACSSAQTSPTEETNLPTPASVFCEEN
                     SGVLEIRQDTDGNQSGVCIFKDGSECEEWAFYRGECAPGEAKTTPTAPAAESTAAREG
                     SPEVVSDGCSIYRNETLGYSFHYPAGSTIVQNDEPAKSYTITGPVVNGETWPMFTISH
                     PADRTEYRPPEGVDLEQWLSEHYLVGENRQPDLTIAGVPAIHYRHERSPQSYADDRYY
                     FAYQGQLYMILIGHSGDHEDWELCDHFMESFQFGPA"
     gene            complement(1062..1739)
                     /locus_tag="VN363_05255"
     CDS             complement(1062..1739)
                     /locus_tag="VN363_05255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83951.1"
                     /translation="MRYFLKSVLGIGAFVLLLLGGLILVRNINRQPSVVLSDDTCQPP
                     CWKTIQPGVTTGDEVYSILSEMEGVSLLQDNLTLDGTLRSISWLYNRPVEDTGGQIYF
                     QDDRVALISILTVNSLQLGDITARLGQPEQYWTALGRGETREYVTVGLLAPAQGYQVE
                     LVINLQNNASSVLIKDSTPVFKVKYFDPARLGELLGKNDLIPNTAVQDTENLPAWTGP
                     GRLEIPR"
     gene            complement(1866..>2170)
                     /locus_tag="VN363_05260"
     CDS             complement(1866..>2170)
                     /locus_tag="VN363_05260"
                     /inference="COORDINATES: protein motif:HMM:NF019844.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HWQ83952.1"
                     /translation="ATLRIGPEPEPTPTSAPTPTPRPRKLAIVLGDSINVRSGPGKEY
                     DILGSADKGEELEARGVSSDQDWFLIYTPDLGEGWVSAPLVSLRVPVEELPVVRNP"
BASE COUNT          489 a          614 c          637 g          430 t
ORIGIN      
        1 gttgatgtgt gagaagggca ttcttacgca gggaacgcag acataccaaa ccccaggggt
       61 ctctgagacc cctggggtct gaggagaagg ccttccaatc aggaccggtt acccatttaa
      121 aacgcggaaa ggacaggggt cgatatagga tctaatgagg tgtgaccaag tggaacattt
      181 cacaaacggt cagcgtcttg caaataatca ctggacaaac caggtgcggc agcgttcccg
      241 tcgcatgcag gagttaaaag atgtcccgaa tcatacgcat aacgatctat tacatgctgc
      301 tcctggtgct ggccgcctgc agcagcgctc aaaccagccc gactgaagaa acaaacttgc
      361 ccacccctgc atccgttttc tgcgaggaga acagtggggt gctggagatc cgccaggata
      421 cggatggcaa ccagtcgggc gtctgtatct tcaaagatgg cagcgagtgc gaggaatggg
      481 cgttttaccg cggggagtgc gcgcccgggg aagctaaaac aactcccact gcgccagcag
      541 ccgaatccac cgcggccaga gaaggctccc cggaggtggt cagtgatggc tgttccatct
      601 accgcaacga gacgctgggc tacagcttcc attacccggc cgggtcgacc attgttcaga
      661 atgacgagcc ggcgaagagc tatacgatta ccggaccggt ggtcaacggc gaaacctggc
      721 ctatgttcac gatcagccac cccgccgacc gcacagaata ccgccctccg gagggtgtgg
      781 acctggagca gtggctgagc gaacactatc tcgtcgggga gaaccgccag cccgacctga
      841 cgatcgcggg cgtgcctgcc atccattacc gccacgagcg cagcccacag tcctatgccg
      901 acgaccgcta ttactttgcc tatcagggcc agctgtatat gatcctgatc ggtcacagcg
      961 gagaccatga agactgggag ctgtgcgatc actttatgga gagcttccag ttcggacccg
     1021 cataaaagcg gattatagat actggactgg ctattcgatc ctcaacgcgg aatttctagc
     1081 ctgcctggcc ctgtccaggc aggcaagttt tcggtgtcct ggacggcagt attggggatc
     1141 aggtcatttt ttcccagcag ctcacccagc ctggccgggt cgaaatattt gactttgaag
     1201 accggggtgc tgtccttgat gagcacggaa ctggcattat tctgcaggtt aatcaccagc
     1261 tctacctggt aaccctgagc aggcgccagc aagcccaccg tcacatattc gcgggtttca
     1321 ccccgcccga gagctgtcca gtactgctcc ggctggccca gcctggcggt gatgtcgccg
     1381 agctgcaagg aattaaccgt caggatagaa atcaacgcca cgcgatcgtc ctggaaatag
     1441 atctgcccgc ccgtatcttc caccggacga ttgtaaagcc aggatatgct gcgcagggtg
     1501 ccatccaggg tgaggttatc ctgcagcagg ctgacgcctt ccatctcact caggatggaa
     1561 tagacttcat cgccggtggt cacgccgggc tggatggttt tccagcaggg cggctggcag
     1621 gtatcatcgc tgagcacgac ggaaggctgg cgattaatat tgcgtaccag gatgagcccc
     1681 ccgagcagga gcaggacgaa ggcgccgatc ccaaggacgg atttcaggaa gtatcgcatg
     1741 cgtttgatta tagtcaatat tggttaagaa tcagatctgg caagcggttg ccagatctga
     1801 ttcaggggca ggggaggcaa atccgaatac cccgcaagat gtgtagcgac aaccccggct
     1861 gtgtttcagg gattcctgac aactggcaat tcttctacag ggacgcgcag cgagaccagc
     1921 ggcgccgaga cccagccttc acccaggtcg ggtgtgtaga tcaggaacca gtcttgatca
     1981 gatgagacac cgcgcgcttc cagctcttcg cctttgtcgg cgctgcccag aatatcatat
     2041 tccttccccg gtccgctgcg cacattgatt gaatcgccca gcacgattgc cagcttgcgc
     2101 ggcctggggg tgggggtcgg cgcactggtc ggggtaggct ctggctcagg accgatgcgc
     2161 agcgtagcag
//
LOCUS       DATMXK010000285         4692 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_80074, whole genome shotgun sequence.
ACCESSION   DATMXK010000285 DATMXK010000000
VERSION     DATMXK010000285.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4692)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4692)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4692
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_80074"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..311
                     /locus_tag="VN363_05265"
     CDS             <1..311
                     /locus_tag="VN363_05265"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558716.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase RNA specificity
                     domain-containing protein"
                     /protein_id="HWQ83953.1"
                     /translation="TVLIVSWQAPNTLGRRLADREAEVRIFGEVYQRRAEVATIGGLS
                     AHAGQALLVEYALATKETVQNIYLVHGEAKPAGALIGKLAENGLRNVDYPGLGSGVDI
                     "
     gene            364..450
                     /locus_tag="VN363_05270"
     tRNA            364..450
                     /locus_tag="VN363_05270"
                     /product="tRNA-Ser"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:398..400,aa:Ser,seq:cga)
     gene            complement(597..2258)
                     /locus_tag="VN363_05275"
     CDS             complement(597..2258)
                     /locus_tag="VN363_05275"
                     /inference="COORDINATES: protein motif:HMM:NF012464.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWQ83954.1"
                     /translation="MNRYKQREDSSPLPGAAYIRFSSEMQSDSFSLDAQLRQIKEQAE
                     RDGVEIVKVFADPAQSAYRKKYRPGINAMREAARRGEFKVLYVHKVDRLARRLEWSLE
                     IVHELQELDIIFKAVQQPFDLGTPEGKLLFHLVSSLGEFYSDNLSKETNKGKLERSLQ
                     GYHNGTVPWGYISVLQGNRKVGVPDPEKAPIVVAMFERFATGQYSDMQIAAWLNEGGY
                     LTAKNRMFNKDSVRDMLCNPYFVGKIRYRGMTVRPKGVSYRSTPPQVSDGQHEPIITQ
                     ELWDRCQALRASRRVVPKPGQKAARVHLLQSLAVCAHCGRRLRVQTPKNYPTYYREDS
                     HLRGYYDCPYSGQSIHADRLDEQVAALIQSLKLTATWEEDVRKLLHDGQDRPDPETER
                     KEIRSMLRLMRDNYERGLYEGEEYQYWQKVNSLKEKLELLTRIPESAIERAALTLLNL
                     HDSWEWATREERKMLVRTMIQEVGCDVGTKRIMWVKVNPDYEILFRLMDRLRPDAGRR
                     YWIREQGAEGNIVDIGEEMRQTLTEVKIAFPVSHNTLTIAEEYVQ"
     gene            complement(2365..2565)
                     /locus_tag="VN363_05280"
     CDS             complement(2365..2565)
                     /locus_tag="VN363_05280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83955.1"
                     /translation="MADEHVSQNFVKNPDPQVNQVSGDDITPSQEQQPLPDRLPQSLI
                     EWLARINRRYLALNQEKTNDTS"
     gene            complement(2696..3136)
                     /locus_tag="VN363_05285"
     CDS             complement(2696..3136)
                     /locus_tag="VN363_05285"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83956.1"
                     /translation="MWRLGQTQPVKAVFAVYKDAMEAQALALMGRKMRAAQTLYGDEV
                     GGAIVPAEEGDFITELAREVLRGAELDDLQSLFADELRVSNSPMGCPTEISPVLIPVS
                     PKTWLDWALEHQAAKAAAGRRSAGKRSNIVQPGQLSIWNNPKEE"
     gene            complement(3263..>4692)
                     /locus_tag="VN363_05290"
     CDS             complement(3263..>4692)
                     /locus_tag="VN363_05290"
                     /inference="COORDINATES: protein motif:HMM:NF012307.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="reverse transcriptase domain-containing protein"
                     /protein_id="HWQ83957.1"
                     /translation="RPGRGCHTALANLHKKFQGSAWFIEGDIRGCFDNIDHESLMEIL
                     ARDIQDGRLLNLIRMGLEAGYMEDWQYNRTYSGTPQGGILSPLLSNIYLHELDAYVED
                     VLIPQYTRGKRRAHSLEYCRMSYPIQCARQAGDTQRVKQLELERRKLPSQDVHDPNYR
                     RLQYIRYADDFILGFIGPKSEAEQIKAAIGAFLKDRLLLEMSASKTLITHARTEHARF
                     LGYDISVYYSDDKLSPRTGTLTKTRSINGCIRLGIPFGKVDELARRYLRGAKPIHEAG
                     LLAYSDGQIMDVYQQRFRGLSEYYKYAADRKALRKLKYVMEIALTKTLANKFRTTVPK
                     IYQKYGGKRNVDGYTYKVLVVEVPTKNGTRYIYWGGIPLKVVKPGTQPIDDNIGTRNF
                     ALSSRTDLIQRLQANECEICCSRENCEVHHIRKLSDLKNRWRGRKEKPDWVIRMIAMQ
                     RKTLVVCVKCHDTIHNGKPLPNRSM"
BASE COUNT          931 a         1280 c         1320 g         1161 t
ORIGIN      
        1 atacggtgct gatcgtttca tggcaagcgc caaacacgct gggccggcgg ttggcggacc
       61 gggaggcaga ggtgcggatc tttggtgagg tgtaccagcg gcgggcagag gtggcgacga
      121 tcggggggct ttcggcgcac gcggggcagg cgctgctggt ggagtacgcg ctggcgacaa
      181 aggagactgt gcagaatatt tacctggtgc atggggaggc gaaaccggcg ggggctctga
      241 tcgggaagct ggcggagaat gggctgcgga atgtggatta tccaggattg gggtcgggag
      301 tggatattta gggggagttg ggctataatc tgtgcgctgc ctgtgaagag ccaggctaca
      361 ataggagagg tgccagagtg gccgaatggg acggtctcga aaaccgttgt ggggcttgcc
      421 ccaccgtggg ttcgaatccc accctctccg ctgagcgtaa agtcttgtga gccaaccggc
      481 tctcgatctg ttaaaaaacg gatgtgaaag aacgaggttt tacgctttgg cgtgatcttc
      541 acgcccgaga tcgctcagtg tccgctgggc gatctcaact tttaagcgat actccttcat
      601 tgtacgtact cctcggcgat tgtcaaggtg ttgtgggaca ccgggaaggc gattttgact
      661 tccgttaacg tctgtctcat ctcttcccct atgtcaacaa tattcccttc tgcaccttgt
      721 tctcggatcc agtagcgtcg cccggcgtcg gggcgcagtc tatccatcag ccggaagaga
      781 atctcatagt ccggattgac cttgacccac atgatgcgct ttgtacccac atcgcaacca
      841 acttcctgga tcatagtccg caccagcatc ttgcgctctt cgcgggtagc ccattcccag
      901 ctgtcatgca gattcagcag ggtgagtgca gcccgttcaa tcgccgactc cgggatgcgg
      961 gtgagcagct cgagtttttc cttcaagctg ttgactttct gccagtactg gtattcttcg
     1021 ccttcgtaca ggccccgctc atagttatcg cgcatcaggc gcaacatgct gcggatctcc
     1081 ttgcgctcag tctccgggtc gggtctatcc tgtccatcat gtaggagttt acgcacatcc
     1141 tcctcccagg tggcagtcag tttgagtgac tgtatcagag ccgccacctg ttcatccagc
     1201 ctgtcagcgt gaatgctttg acctgagtac gggcagtcgt agtaaccacg caggtgcgaa
     1261 tcctcccggt agtaggtggg gtaattcttc ggtgtctgaa cgcgaagtct tcgaccacaa
     1321 tgcgcacaga ccgctaagct ttgcagcagg tgaaccctgg cagccttctg cccgggtttg
     1381 ggaacaacac gccggctagc acgtagtgcc tgacagcgat cccacaattc ctgggtgatg
     1441 attggttcgt gctggccatc agagacttgg ggcggtgtgg agcgatagct gacccccttt
     1501 gggcgtactg tcatgccacg gtaacggatc ttgccgacaa agtagggatt gcagagcata
     1561 tcccgcaccg agtccttgtt gaacatgcga ttctttgcag tgaggtatcc accttcgttc
     1621 aaccaggcgg ctatctgcat gtccgaatac tgcccggtgg cgaaacgctc gaacattgcc
     1681 actactattg gtgctttttc tggatcaggg acccctactt tacggttgcc ctgcagcacg
     1741 ctgatgtagc cccaaggaac tgtgccgttg tgatagcctt gcagcgaacg ctccagcttg
     1801 cccttgttgg tctcttttga caaattatcg ctatagaact cgcccaggga gctgaccagg
     1861 tgaaagagca gcttgccttc cggtgtaccc agatcgaagg gctgctggac agctttgaag
     1921 atgatatcca actcctgcag ctcgtggacg atctccagcg accattccag gcggcgcgcc
     1981 aggcgatcca ctttatggac gtacagcacc ttgaactcgc cgcgccgggc agcttcgcgc
     2041 atggcattga tcccaggacg gtatttcttg cgataagccg attgggccgg atcggcgaac
     2101 accttgacaa tctcgacccc atcccgctca gcctgctcct tgatctggcg tagctgggca
     2161 tccaggctga acgaatcgct ttgcatctca gatgaaaaac gaatgtacgc tgctccaggg
     2221 agtggggaag agtcttctct ctgcttgtat cggttcatct tattcaatcc tttcgttcaa
     2281 aatagtagtg tggagtatta acagagaaaa ctatccagaa agtttcatgg cacacctcca
     2341 atccaggagc agccagattg atcattagct ggtgtcgttc gtcttttcct ggttgagcgc
     2401 caggtaacgg cggttgatgc gcgctagcca ttcgatcagg ctttgaggga gcctgtctgg
     2461 taagggttgc tgctcctggg aaggggttat atcatctccg gaaacctgat taacttgtgg
     2521 gtcaggattt ttaacaaagt tttgacttac atgctcatct gccataattg cacctcatac
     2581 gataagaagt attgaatgtc ccttatgcac cttagggggt aaaagaaaac tccggttctg
     2641 gcagattggc tgccaaaacc ggagcgttga ttgtgggata tcgttgtagt ttgggttatt
     2701 cctccttcgg gttattccaa atcgataact ggcctggctg gacgatattg ctccgtttac
     2761 cagcggacct tctaccagca gccgctttgg ctgcctggtg ttccagcgcc caatccagcc
     2821 aggtcttggg actcacgggg atcagaaccg ggctgatctc ggtcgggcag cccatcggtg
     2881 agttcgacac ccgcaactca tcggcaaaga gcgactgcag atcgtccagc tcagcaccgc
     2941 gcaacacttc acgggcaagc tcggtgatga agtcgccttc ttcagcgggt acgatcgcac
     3001 cgccgacttc gtcgccatac aacgtctggg cagcccgcat cttgcgcccc atcagggcca
     3061 gcgcctgggc ttccatggcg tctttgtaaa cggcaaacac cgccttgacc ggctgagtct
     3121 gccccaatcg ccacacccgc cgcagcgcct gccaaagggt gtagtagggt aggaaagtgg
     3181 cgagttacct tcacaggcac ttttccacag acccccctcc gaaccggacg tgatactttc
     3241 gcatcatccg gctctccaga actcacatgc ttcggttggg taacggtttc ccgttgtgga
     3301 tcgtatcgtg gcatttcaca cacaccacca gcgttttcct ttgcatggcg atcatcctta
     3361 tcacccagtc gggtttctct ttccttcccc gccaacggtt cttcaggtcg gacagcttgc
     3421 gaatgtgatg gacttcgcag ttctctcgtg aacagcaaat ctcacattcg ttagcttgca
     3481 accgctggat caggtcggta cgtgagctga gcgcaaagtt ccgcgttccg atgttgtcgt
     3541 caatcggctg tgtgccgggt ttgacgacct tcaaggggat gccgccccag tagatatacc
     3601 gtgtcccgtt ctttgtgggt acttccacga ccagaacctt gtaggtatat ccatccacat
     3661 ttcgctttcc cccatacttc tggtagattt tggggacggt ggttcggaac ttatttgcca
     3721 gcgtcttggt gagagcaatt tccatcacgt acttgagctt gcgcagcgct tttctgtccg
     3781 cagcgtactt gtagtattca gacagcccac gaaaccgctg ctggtaaacg tccatgatct
     3841 ggccgtccga gtacgccagc aaaccggctt cgtgaattgg ctttgcgcca cgcaggtagc
     3901 gcctggcaag ctcatcgacc tttccaaacg gaatgccgag ccggatgcag ccgttgatac
     3961 tgcgggtctt ggtgagcgtc ccggtccggg gtgaaagttt gtcgtccgaa tagtaaacgc
     4021 tgatgtcgta gcccaaaaat cgggcatgtt cggtgcgtgc gtgggtgatg agggttttgg
     4081 atgcactcat ctctagaagt agcctgtctt tcaggaatgc gccgatagct gctttgatct
     4141 gctccgcttc tgactttggg ccgataaagc ccaggataaa gtcatcggcg taacggatgt
     4201 attgcagccg ccggtagttc gggtcatgca catcttgcga gggaagcttt ctgcgttcga
     4261 gttcaagctg cttcactcgt tgtgtatctc cggcttgccg ggcgcattgg atcggatagg
     4321 acatccggca gtattccagg ctgtgcgctc gccttttccc acgggtgtat tgtgggatga
     4381 gcacatcttc gacataggca tccagttcat gcaggtagat gttggaaagg agcgggctga
     4441 ggataccgcc ctgcggcgtt ccgctgtagg tgcggttgta ctgccagtct tccatatacc
     4501 cggcttccag gcccatccgg atcaggttga gcaagcggcc atcctggata tcccttgcga
     4561 gaatttccat caggctttca tggtcgatgt tgtcgaaaca gccccggatg tcgccctcga
     4621 tgaaccacgc cgatccctgg aacttcttat gcaggtttgc cagtgctgta tggcagccgc
     4681 gtccgggccg ga
//
LOCUS       DATMXK010000286         2285 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_160627, whole genome shotgun sequence.
ACCESSION   DATMXK010000286 DATMXK010000000
VERSION     DATMXK010000286.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2285)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2285)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2285
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160627"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..561
                     /gene="phoU"
                     /locus_tag="VN363_05295"
     CDS             <1..561
                     /gene="phoU"
                     /locus_tag="VN363_05295"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWQ83958.1"
                     /translation="RRVYAGDAEINAKRFELENETMVVLATQQPIMAHDLRLLASILE
                     VTGELERMGDYAKGIAKIALIVGDQPPIKPLIDIPRMSEIAIAMLHRAVSAFVRGDAA
                     DARQIPAIDDQVDALYNQVYRDLTAIVIKDPSAIDRANYLMWAAHNLERMADRVTNIC
                     ERTIYIVTGDLKEMGISDDEILSRHP"
     gene            558..986
                     /locus_tag="VN363_05300"
     CDS             558..986
                     /locus_tag="VN363_05300"
                     /EC_number="1.20.4.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558477.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030612 - arsenate reductase (thioredoxin)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arsenate reductase ArsC"
                     /protein_id="HWQ83959.1"
                     /translation="MNSETTPRKVLFLCTGNSCRSQMAEAIVNARLGDRWQAFSAGTV
                     PAGFVHPLALRALAEIGIDHAGRSKHVNEIRPIRFDLVVTVCDTAAEECPVWLGPGIR
                     THHSFADPAKATGSEEEVMQVFRQVRDQIAEEIPELLKQY"
     gene            1363..>2285
                     /locus_tag="VN363_05305"
     CDS             1363..>2285
                     /locus_tag="VN363_05305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83960.1"
                     /translation="MITCERCKTVNPPELRHCQNCHRDLLPGEGGATRLIILLVAGLI
                     TSVGVWVLARFGQGEPLPDLGGAFSSPVFWAILVIVTPLSGLKMALSPTPAHQRYLNR
                     ARRHLNLDKAQAMADLNQALALAPEKERTAILKERAQLYASLGQVQQATRDRIATIES
                     DGSYQGAEAVAALTGLDKDAFAGGMKDRDIQALLKAQAALSMGYCPKCKNVVELDGKM
                     HCKLHRRARIESVRLAIPEDVSKIKIAILEAHARAARLNRYREIIQITGLIALLVLTP
                     YGLKIGPFAPREAEPASSEIEPTADLDVPVAA"
BASE COUNT          504 a          698 c          616 g          467 t
ORIGIN      
        1 cgccgggtct atgccggaga tgccgagatc aacgccaaac gcttcgagct ggaaaacgag
       61 acgatggtcg tgctggccac ccagcagccg attatggccc acgacttgcg cctgctggct
      121 tccattttgg aggtgaccgg cgagctggaa cggatgggcg attatgccaa gggcattgcc
      181 aagatcgcct tgatcgtcgg tgaccagccg ccgatcaagc ccctgatcga tatcccgcgc
      241 atgagtgaaa ttgccatcgc tatgctccat cgcgccgtca gcgcgttcgt gcgcggcgat
      301 gcagccgacg cccggcagat cccggccatc gatgaccagg tggatgccct ttataaccag
      361 gtctatcgcg atctgaccgc catcgttatt aaagatccct ccgccattga tcgggccaac
      421 tacctgatgt gggcagctca caatctggag cggatggcgg accgggtaac caatatttgc
      481 gagcgaacca tctatatcgt taccggggat ttgaaagaga tgggaatatc cgacgacgaa
      541 atcttaagca ggcatccatg aacagtgaaa ccactccccg gaaagtcctc tttttatgca
      601 ctggaaattc gtgccgttca cagatggcgg aagcgattgt caatgcccgg ctgggcgacc
      661 ggtggcaggc attcagcgcc ggcacagtcc ctgccggctt tgtccacccg ctggcgctgc
      721 gcgctctggc agagatcggt attgaccatg cagggcgctc caagcatgtc aacgagatcc
      781 gtcccatccg cttcgatctg gtggtcactg tctgtgatac tgcggccgaa gaatgccctg
      841 tctggctcgg tcccggcatc cgcacccatc atagctttgc tgatcccgcc aaagccaccg
      901 gcagcgagga agaagtcatg caggtcttcc gccaggtccg cgaccagatt gcagaagaaa
      961 tccccgaact ccttaagcag tactgacttc ttaatcacga attcggacag actcaagcct
     1021 atcctacatc cgtaggtcat gctttagctt gactcaacct gacaggccga agcctatcct
     1081 acaccgtagg ctatgcttta gctttactca aacagacagg ctagagccgg gcctaccccg
     1141 taggtcatgc tttagcttga ctcacccaga caggctcaag cctatcctgc gtccgtaggt
     1201 catgctttag cttgactcat cctgacaggc tgaagcctat cctacaacct accgaatgac
     1261 gaaatcatct attgacatat ccataaattt cggtattctg tgaataccga cacaattcga
     1321 tgttttctga ttccactatt tgattcggcg ggggaaactt ggatgattac ctgcgagcga
     1381 tgcaaaacag tcaatcctcc cgagcttcgc cactgccaga actgccatcg cgacctgctg
     1441 ccgggcgaag gcggtgcaac gcgcctgatt attctccttg tcgccgggct gatcacctcc
     1501 gtgggagtgt gggtgctggc gcgcttcggg cagggtgagc cactgccgga tctgggcggt
     1561 gctttctctt caccggtatt ctgggcgatc ctggttatcg tcaccccgct cagtgggcta
     1621 aaaatggctt tatcgcccac tcccgcccac cagagatatc tcaatcgcgc caggcgccac
     1681 ctcaatctcg ataaagctca ggcgatggct gacctcaacc aggccctcgc gctggcgccc
     1741 gaaaaggaac gcaccgccat cctcaaagag cgcgcccagc tttacgccag cctgggacag
     1801 gttcagcaag ctacccgcga caggatcgcc acaatcgaaa gtgacggcag ctaccagggc
     1861 gcagaagcgg tcgctgccct gaccgggttg gacaaggacg ccttcgccgg cggcatgaaa
     1921 gacagggata tccaggcctt gctgaaggct caggcagccc tctccatggg ctactgcccc
     1981 aaatgtaaaa atgtggtcga gctggacggg aagatgcact gcaagctcca ccgccgcgcc
     2041 cggatcgaaa gcgtgcgcct ggcgataccc gaagatgtct cgaagattaa aatcgccatc
     2101 cttgaagcgc atgccagagc tgcccgcttg aatcgctacc gggagatcat ccagatcacc
     2161 ggtttgattg ccctgctggt tctgacaccc tacgggctca aaatcggtcc ctttgccccg
     2221 cgggaagcag aaccggcgag cagtgaaatc gaacccactg cagacctcga tgtgccggtg
     2281 gcagc
//
LOCUS       DATMXK010000287         2871 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_84410, whole genome shotgun sequence.
ACCESSION   DATMXK010000287 DATMXK010000000
VERSION     DATMXK010000287.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2871)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2871)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2871
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84410"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(205..843)
                     /gene="rpsD"
                     /locus_tag="VN363_05310"
     CDS             complement(205..843)
                     /gene="rpsD"
                     /locus_tag="VN363_05310"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004410410.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0015935 - small ribosomal subunit
                     [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_function: GO:0019843 - rRNA binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S4"
                     /protein_id="HWQ83961.1"
                     /translation="MAKYTGPVCKLCRREGEKLFLKGERCFTPKCAFEKRDYAPGEHG
                     KSGQFRRRRDSDYNRQLRAKQKARRIYGIYERQFRRYYEVSLGRRGLTGMNLLQILES
                     RLDNVIYRLGFASSRAQARLLVTHGHFSVNGRRTDIPSMLMDAGDIVSVRDGSRKREF
                     FKTLPDYAEARNAPVWLSRDVKALSGTVLRLPERAEIDGNLQEQLIVEYYSR"
     gene            complement(878..1282)
                     /gene="rpsK"
                     /locus_tag="VN363_05315"
     CDS             complement(878..1282)
                     /gene="rpsK"
                     /locus_tag="VN363_05315"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001029758.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S11"
                     /protein_id="HWQ83962.1"
                     /translation="MAQSVRATRRKGGVKKVKRTLSSGQVHVFATFNNTIVTITDDQG
                     NTLAWGSAGSAGFKGSRKSTPFAARLAAEQAIKAAMAMGLQEVALIVKGPGPGRESAI
                     RAVQSQGLRVTSISDITPVPHNGCRPPKKRRV"
     gene            complement(1416..1802)
                     /gene="rpsM"
                     /locus_tag="VN363_05320"
     CDS             complement(1416..1802)
                     /gene="rpsM"
                     /locus_tag="VN363_05320"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559453.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S13"
                     /protein_id="HWQ83963.1"
                     /translation="MARIEGVDLPRNKRVEIGLTYIYGIGLPRAHKILAETKVNPDTR
                     IKDLTDAEVNLLRDSISQSYKVEGDLRREVQLNIKRLIEIGSYRGIRHRRNLPTRGQR
                     TRTNSRTAKGPKKTVAGRGRRRGAKK"
     gene            complement(1808..1921)
                     /gene="rpmJ"
                     /locus_tag="VN363_05325"
     CDS             complement(1808..1921)
                     /gene="rpmJ"
                     /locus_tag="VN363_05325"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003720928.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L36"
                     /protein_id="HWQ83964.1"
                     /translation="MKVTASIKKRCAKCKVVKRKGILFVICENPKHKQRQG"
     gene            complement(2044..2820)
                     /gene="map"
                     /locus_tag="VN363_05330"
     CDS             complement(2044..2820)
                     /gene="map"
                     /locus_tag="VN363_05330"
                     /EC_number="3.4.11.18"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559451.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I methionyl aminopeptidase"
                     /protein_id="HWQ83965.1"
                     /translation="MMSWERSITLKTPHEIEIMREAGKINAEALAAAKNAIRPGATTA
                     DLDAAAAEVLRKHGAKAAFLGVPGAFPYPATTTISINDEMVHGIPSHKRKLVEGDIVS
                     IDCGTILDGFVADSAISAGVGEISEQAQRLLEVTEKSLYIGIEQSVPGNRTGDIGHAI
                     ETYVEGEGYYLTHEYTGHGVGRNMWEGPTVPNYGKPGRGVPLRTGMTLAIEPMVLIGT
                     ERTVILEDHWCVASADGSLTCHFEHTLAITEAGPVILTLP"
BASE COUNT          601 a          825 c          704 g          741 t
ORIGIN      
        1 taacgtcacg ccatatccac gttccagcgg gctgatgatg aacttaccgt aattgcgcgc
       61 ttctgctttg cgctcaattt tcggcgctac catgtttgac aaaccaatca cggttcacct
      121 cttcccctag ttcaaattcc attaccggcg gctcgttcag gagctagtcg ccggggtttt
      181 tattcaggcc tgcagttcgc gggtttagcg tgagtaatac tcgacgatca actgctcttg
      241 cagattgcca tcaatctcgg ctcgttcggg taaccggagg actgtccccg aaagcgcctt
      301 cacatcccgg ctgagccaca cgggagcgtt gcgcgcctcc gcataatctg gcagggtctt
      361 gaaaaattcg cgctttcgtg atccatcccg gacggatacg atgtcgcctg catccatcag
      421 catggaggga atatccgtgc ggcgaccatt gaccgagaaa tggccatgag tcaccagtaa
      481 acgcgcctgg gcgcggctgc tggcaaatcc aagccggtag attacattat cgaggcggga
      541 ttcgaggatc tggagcaggt tcatacctgt caacccacgg cgtcccaacg agacttcata
      601 atagcggcgg aactgtcgtt cgtagatgcc gtatatccgg cgagcctttt gtttggcgcg
      661 cagctggcgg ttatagtccg aatcgcgacg gcgacggaac tgtccactct tgccatgctc
      721 accaggcgca taatcccgct tctcgaaagc gcatttggga gtgaagcaac gctcgccttt
      781 aaggaataac ttttcacctt cacggcggca caacttacat actggccctg tatacttagc
      841 catgtctttt tatcctattc cttcctgggt tctttgccta gacgcggcgc tttttgggcg
      901 gacggcagcc attgtgggga accggggtga tatcggatat cgaagtcacg cgcaagccct
      961 gagactgaac agcacggatg gccgattcac gcccaggacc aggacccttt acgatcaaag
     1021 cgacttcttg caggcccatt gccatcgcag ccttgatagc ctgttcagca gccagccgtg
     1081 cagcaaaagg agtgcttttc cgagaacctt tgaaaccagc cgatcctgcg ctaccccagg
     1141 caagtgtgtt gccctggtcg tcagtgatcg tcacaatggt attgttgaag gtagcgaaca
     1201 cgtgtacctg tccagaagac agggttcgct ttactttctt tacgccgccc ttgcggcgcg
     1261 tcgcacgtac actttgagcc ataaaacctc acttattggt tgaattcgca gttaaattct
     1321 tatctgctca ctcacaactg gtgaattgct gccaggccag ccagccgctg ggaggaagga
     1381 acccaagcca gccagcatca accgccagta gtctactatt tcttggcgcc acgtcgccgg
     1441 ccgcgaccag ccacagtttt cttggggcct ttcgcagtcc gggaattggt gcgcgttcgc
     1501 tgtccacggg ttggtaaatt acgccggtgg cgtattccgc gatagctgcc gatttcgatc
     1561 aagcgcttga tattgagctg gacctcgcgc cgcagatcac cctcaacctt atagctctgg
     1621 ctgatggaat cacgtagcag gttcacctct gcatccgtta aatccttgat ccgggtatcc
     1681 gggttcacct tggtttctgc caggattttg tgcgcccggg gtaaaccaat tccatagata
     1741 tatgtcagac caatttctac ccgcttgtta cgcggtaagt caacgccttc aatacgtgcc
     1801 atatttatta tccttgtcgc tgcttatgct tcggattttc acaaatgaca aataaaatgc
     1861 ccttgcgctt gacgacttta cattttgcac agcgtttctt aattgatgca gttaccttca
     1921 taacgccgat ctccttctta cattcgctgt tctgaaccta ccggtctttt tccgattgtc
     1981 agtccagcca aagccttgat tatactacct tcgttttatc ccgtccagac tcacttacca
     2041 ttgttatggt agggtcagga tcacaggtcc agcctctgtt atcgccagtg tgtgttcaaa
     2101 atggcaggtt agcgacccat ctgctgatgc cacacaccag tgatcctcca ggatcacggt
     2161 ccgttctgtg cctatgagta ccatcggttc gattgccagg gtcatccccg ttcgcagggg
     2221 tacaccgcgg ccaggtttcc cgtagttcgg tacagtagga ccttcccaca tgttccggcc
     2281 aacgccatgc ccggtgtact catgggtcag gtagtaacct tcaccttcta cataagtctc
     2341 gatggcatgt ccaatgtcac cggtgcggtt ccctggaacg ctctgctcga tcccgatgta
     2401 gagcgacttc tcggttacct ccagcagacg ctgggcttgc tcagaaattt ccccgactcc
     2461 agcgctgatt gccgagtcag ccacaaatcc atccaggatc gtaccgcaat cgatcgaaac
     2521 gatatcccct tcgaccagct tacgtttatg gctggggata ccatgcacca tctcatcatt
     2581 gatgctgatc gtcgtggtag cgggatacgg aaatgcaccg gggaccccga gaaacgcggc
     2641 tttcgcgcca tgctttcgaa ggacttctgc agcagctgca tccaggtctg ccgtggtcgc
     2701 gcctggacgg atggcgtttt ttgctgcagc cagagcttct gcattgattt tcccagcctc
     2761 ccgcataatt tcaatctcat ggggagtttt cagagtaatg ctacgttccc aactcatcat
     2821 gtcaatgtgc tcacaaactt agccgttctt ctcaatcgca ttgagtaagt c
//
LOCUS       DATMXK010000288         2218 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_1123, whole genome shotgun sequence.
ACCESSION   DATMXK010000288 DATMXK010000000
VERSION     DATMXK010000288.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2218)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2218)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2218
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1123"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2218)
                     /locus_tag="VN363_05335"
     CDS             complement(<1..>2218)
                     /locus_tag="VN363_05335"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ83966.1"
                     /translation="AGNIAIAVEDTRLYSALRRRAEQLTTISEVSSAITSILNLDELL
                     DQVITLIQKRFDYPYVYLFTVHPRRRKIIFQAGSDPRRNSLRSEGFAFDLDDPQGIIP
                     WAARHAATLLVNDTSQEPRYRPSELPPENTLSELAVPLIFANEVLGVLDIQSDRLNEF
                     SEEDRFLFEALADTIAVAMRNAFLYRTEKWRRQVSDSLREVAGLLSADVDLDQVLDAI
                     LAELERTLPCDVAAIWLLDEFEIADSMPPGQPHLRLGAVLGVEKAAVELTRGIHLEDL
                     VVKLQQDQAVSGDDDLTVWLIQALDGNAPLIRSSKSPYEPLGRAFRFPPDYSAIASPL
                     RVGEQVLGLLVLGHHTPGRYGSEAQAMTAAFASYASVAIENTRLYEAAHEQAWVSTVL
                     LQVAEASQNLTNQNELLSTIVRITPMLIGVRSCALYIWDDLEEAFSPAAYEGLTTEQK
                     DEFERWRFAAGDSAILDRLRLEKKPIILHGLEEDPHLEGVLFDNQAPALLPGAELLVL
                     IPMISRGEVGGAFLVDYTSDLGNEAHPEMIMSQFDERLAIIQGIAHQTAVAVENIRLQ
                     KAQREEAYVSVALLQVAQAVTSLTDLDDILSSIVRITPILVGVRRSMIFLWQEADASF
                     LLAESYGLPRTVNGRSYPVGEFDLLDSALQLLNPVAFPADNPELLTPELAGIEPPDLW
                     TEIVLEDVDVVEEYLESDLRLLIALPLIAKGEILGVMLVEEPEVDSIGGREGGRALR"
BASE COUNT          544 a          634 c          572 g          468 t
ORIGIN      
        1 gcgcaaggcg cgaccgcctt cgcgcccccc gatggaatcg acttctggct cctcgaccag
       61 catcacgccc aatatttccc ctttggctat gagaggcaaa gctatcaata agcgcaaatc
      121 gctctctaaa tattcctcaa caacatcaac atcctcgaga acgatttccg tccataaatc
      181 aggcggctcg atcccggcca gctcgggcgt gagtagttca gggttgtctg cggggaatgc
      241 taccggatta agcagctgta aagcagaatc gagcaggtca aattcgccca caggataaga
      301 acgcccgttg accgtgcggg gcaagccata agactcagcc agcaggaaac tggcgtcggc
      361 ttcttgccac aagaagatca tgctgcggcg aacgccaacc aggatgggcg taatccggac
      421 gatcgaagag agaatgtcat ccagatcggt caggctggtt accgcctgag ccacctggag
      481 caaggccaca gaaacataag cctcttcacg ttgggctttt tgaaggcgaa tattctccac
      541 tgcaacggct gtctgatggg caatcccttg aatgatcgcc aatcgctcat cgaattggga
      601 cataatcatt tcagggtggg cttcattccc cagatcgctg gtatagtcca ccaggaatgc
      661 gccgcccacc tcaccccgag atatcatggg gattaacacg agcagctctg cccccggcag
      721 taaggctgga gcctggttat cgaataaaac tccctcgaga tggggatctt cctcaagacc
      781 atgcaatatg atcggtttct tctccaggcg tagccggtcc aggatggccg aatctccggc
      841 agcaaaacgc cagcgctcaa attcatcttt ttgttctgta gtaaggcctt cataagcggc
      901 cggtgaaaat gcctcctcca gatcgtccca aatatacaat gcgcaagaac gcacaccgat
      961 cagcatggga gtaattcgca caatcgtgga aagcagttcg ttctggttcg tgaggttttg
     1021 agaagcttct gctacctgca aaaggaccgt cgaaacccag gcttgttcat gagccgcttc
     1081 atacaagcgc gtattttcga ttgccaccga agcgtaactg gcgaaagctg ccgtcattgc
     1141 ttgcgcttct gagccatagc ggcctggcgt gtgatgcccc aggaccaata agcctaatac
     1201 ttgctctcct acacgcaggg gagatgcgat cgctgaataa tcgggcggaa aacgaaaagc
     1261 acgccccagg ggctcgtaag gcgatttact gctgcgaata agcggcgcat tgccatccag
     1321 agcctgaatt aaccagaccg ttaaatcatc atcccctgaa acagcctggt cctgttggag
     1381 cttcactacc aggtcttcga gatgtatacc ccgggtcagc tccactgcag ccttctcaac
     1441 cccgaggact gctccaagtc tcaggtgtgg ctgcccaggc ggcatactgt ctgcgatctc
     1501 aaattcatcc aaaagccaga tcgcggccac gtcgcatggc agtgttcgtt ccagctcggc
     1561 aagaattgca tccagtacct gatccagatc cacatccgcg gaaagcagac cagcgacttc
     1621 acgcaggcta tcggacacct gacggcgcca tttctcggtc cggtacagaa acgcgttgcg
     1681 catcgccaca gcgatcgtgt ccgccagggc ttcgaacagg aagcgatcct cttcactaaa
     1741 ttcattcaga cgatcgctct gaatatcaag gacgcccagt acctcattcg caaagatcaa
     1801 aggcaccgcc aattcggaca gggtattctc gggcggtagc tcagaagggc gatagcgcgg
     1861 ctcctggctg gtatcgttta ccagaagggt tgctgcatgc cgggctgccc aggggataat
     1921 cccctgagga tcatccagat caaaggcaaa accctcactg cgtagtgagt tccggcgcgg
     1981 gtcgctgccg gcctgaaaaa taatcttccg ccgccgcgga tggacagtaa aaaggtatac
     2041 ataagggtaa tcgaatcgct tttggattaa ggtgatcacc tgatccagca attcatccag
     2101 gttcaagatc gatgtaatcg cgctgctgac ctcagagatt gtcgtgagct gctcggcgcg
     2161 gcgcctgagc gcagaataca gccgggtatc ttcgactgca attgctatat tcccggcc
//
LOCUS       DATMXK010000289         3495 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_35464, whole genome shotgun sequence.
ACCESSION   DATMXK010000289 DATMXK010000000
VERSION     DATMXK010000289.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3495)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3495)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3495
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35464"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3435
                     /locus_tag="VN363_05340"
     CDS             <1..3435
                     /locus_tag="VN363_05340"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transglycosylase domain-containing protein"
                     /protein_id="HWQ83967.1"
                     /translation="NRFQRLANSEAELSNQEQNAMAEEENSVPDTSAPIQSEADAYFN
                     EQDSQSFPENSQDEKPGEPPDGVEPDSASPSIESAQPGSQSAESSSPPNAVPPVDLST
                     QPMPLKDEEDTVPLGPGIPTTSHVPFGGSTPPSGMPALDKYGFPLPRRVDETDVNATR
                     VSPAAYGPPPQRPRTAPRPPASVPPPPPVSIPPARSVPPRRRPRRQAGFDFSPNLGCL
                     ARSGLIAAFVFAVILLCGASFVIFQYYRIASDLPDINELRQKAAQFETTRILDRNGNV
                     LYEILDPNAGRRTYVPLAEISPYLVAATIATEDKEFYDHPGFNILAIFRAFWQNYQGG
                     EIVSGASTITQQLARALLFTPEERAEQSYDRKIREAVLAAEITRRYSKDDILELYLNE
                     IYYGNLAYGVEAAAETYFGLRADQLTLGQASLLAGLPQAPSVYDVYTRPDLALQRHEA
                     VLVLMYQASQEQGCIFVSNSPQRVCLDPVVVTQAADEMKNYTFNSPDIQIRYPHWVDY
                     IRALLEQQFDAQIIYRSGFTVYTTLDPGLQDLAEQVVSGQIERLQENRASSGALVAVR
                     PITGEILAMVGSADFYNEAISGQVNMAINPRQPGSSIKPLTYIAAFEKGWTPGTLIWD
                     VPSEFPPLGLPNDPSPVYQPVNYDGRFHGPVTVRTALANSYNMPAVKTLNFVGIYDDP
                     TTASADGFINLAKRMGITTLTRQDYGLSLTLGGGEVTLLELTNAYATIANYGRRVPPV
                     AITRILDKAGEEVYRYNQPSGDQVIRPEHAYLISSILSDNEARSPAFGANSVLNLPFQ
                     AAVKTGTTNDFRDNWTLGYTPDLAVGVWVGNADYTPMVNTSGLTGAAPIWSEFIQTAV
                     GQITGGNPTPFKRPGGIVDHVICAISGTLPSEWCPLQRTESFAADQPPLPREQDLWQR
                     INLDTWTGLRASQDCSGFTEERFVLNVTDEFARQWLRRDAQGEAWAEAAGFGEGLAFT
                     PPRECQAGDPRPILEISSPRPGETILNNPLVINGVANASQWFDFWQLDYGLGNEPVEW
                     KTLQQSKSAFPQAGELFAWYMDDLIRDNISGPLTLRLRVNSTEDTHADLKITLNLSLP
                     TPTPTATPTPTVTILPTVIPTATPTPTIMITREPRETETPTPTSLPDETTPIVPTP"
BASE COUNT          781 a          999 c          895 g          820 t
ORIGIN      
        1 aatcgttttc agcgtctggc taactcagag gctgagctga gcaatcaaga gcaaaatgca
       61 atggcggaag aggaaaattc tgttccggat acatcggcac caattcagtc agaagcggat
      121 gcttatttta acgagcagga cagccagtct tttccggaga acagccagga tgagaaaccg
      181 ggagaacctc ctgatggggt agaacccgat tcggccagcc catctataga gagcgcccaa
      241 ccaggcagcc aatcagctga atccagcagt ccacctaatg ctgttccccc ggttgatctt
      301 tccacacaac ccatgcctct caaagatgaa gaggatacag tccccctcgg acctggcata
      361 ccaacaacct cgcatgtacc ttttgggggc agcacgcctc ccagcggtat gcctgccctg
      421 gacaaatatg gatttccact cccccggcgg gtggatgaga ccgatgtcaa tgctacacga
      481 gtatctcctg cggcgtatgg tccgcctcct cagcgaccca gaacagcccc tcgtccgcct
      541 gcctctgtac caccacctcc accagtctcc ataccgcctg cccggtctgt gcccccaagg
      601 cgcaggccac gccgtcaggc aggatttgat ttttccccca acctgggatg cctggcgcgg
      661 agcggcttaa tcgccgcgtt tgtattcgca gtaattttgc tgtgtggggc ttcattcgtt
      721 atttttcaat attaccggat cgcttccgat cttccagata ttaatgagct gcgccagaag
      781 gctgcccaat tcgagacgac gcgcatcctc gatcgcaatg ggaatgtcct ctacgaaata
      841 ctcgacccca acgcaggccg gcgaacttat gtaccgctcg cagaaatttc gccctatctc
      901 gtcgctgcga cgatcgcgac cgaagataaa gagttttatg atcatcccgg cttcaatatt
      961 ctggcgatct ttcgcgcctt ctggcaaaac taccagggag gcgaaatcgt atccggtgca
     1021 tcgacgataa cccaacagct ggcgcgagcg ctgttgttta cgccagaaga acgcgccgag
     1081 cagtcctatg accgcaagat tcgtgaggct gttctggccg ctgagattac ccggcgttat
     1141 tccaaagatg atatcctcga gctttatctg aacgagatct actacggcaa tctggcctat
     1201 ggggtggagg cggcggctga gacttacttt ggcttgcgtg ctgatcaatt aacccttggg
     1261 caggcttccc tcctggccgg gctgccccag gcgccttctg tttatgatgt ttatacccgt
     1321 cccgatctgg ctttgcagcg ccatgaagcc gtgctggttt tgatgtatca agccagccag
     1381 gaacagggct gtatttttgt gagcaacagt cctcaaaggg tctgcctgga cccggtggtg
     1441 gtcacgcaag ctgcggacga aatgaagaat tacaccttta attcacccga tatccagatc
     1501 cgctatcctc attgggtgga ttatatccgg gctttgttgg agcagcagtt cgacgcccaa
     1561 atcatctacc gctctggatt tacagtttat acaacactgg accccggttt gcaggattta
     1621 gccgaacagg ttgtgagcgg ccagatcgag cgcttacagg agaaccgggc ttcatcgggt
     1681 gctctggttg cagtgcgacc gattaccggt gaaatcctgg cgatggttgg atcggcagat
     1741 ttttataacg aagcgatctc cgggcaggtc aatatggcga tcaacccacg ccagccaggg
     1801 tcctcgatta aacctctgac ttatattgcc gccttcgaaa aaggctggac tcccggaact
     1861 ttgatctggg atgtgccttc cgagtttcct cctttagggt tacccaatga tccgagcccg
     1921 gtttaccagc cagtcaatta tgatggtcgt tttcatggtc ctgtcaccgt ccggactgcc
     1981 ctggcaaatt cgtataatat gccggcagta aaaacgctga atttcgttgg gatttatgat
     2041 gacccaacta ctgcttcagc cgatggattt atcaacctgg ctaaacggat ggggatcacc
     2101 acgctgaccc ggcaggatta tggtctttca ttaaccctgg ggggcggtga agttacattg
     2161 ttagagttga ccaacgctta tgccacgatc gccaattatg gcaggcgcgt tccaccggtc
     2221 gcgatcacac gcatcctgga taaagctggc gaagaagtct atcgttataa ccagccgtcg
     2281 ggcgaccagg taatccgccc cgagcatgcc tatctgatct catcaatcct ctcggataat
     2341 gaggctcgct caccggcttt tggtgcgaac tctgtgttga atctcccttt ccaagcagcg
     2401 gttaaaaccg gtacgaccaa tgatttccga gataactgga ctctgggata cacacccgat
     2461 ctggctgttg gagtgtgggt ggggaatgcg gattacacgc ctatggtcaa taccagcggt
     2521 ttgactggcg cagccccgat ctggtctgaa tttattcaaa ccgcagtagg ccagattacc
     2581 ggcggcaatc caacaccttt caaacgaccc ggggggatcg tggaccatgt tatttgcgcg
     2641 atctcgggga ctcttccctc cgaatggtgc ccacttcagc ggaccgaatc attcgccgct
     2701 gaccagccac cgctccctcg ggagcaggat ttgtggcagc gcatcaacct ggatacctgg
     2761 accggattac gggcctccca ggattgctct ggattcaccg aggagcgttt tgtcctgaat
     2821 gtaaccgacg aattcgccag gcagtggctg cggcgtgacg cgcagggtga agcctgggca
     2881 gaagcagctg gctttggcga aggtcttgcc tttacaccgc cgcgtgaatg ccaggctggc
     2941 gacccacgtc caattttaga gatttcttcg cctcgtccgg gagaaacaat cctgaataat
     3001 cctctggtga tcaatggggt tgccaatgcc agccagtggt tcgatttctg gcagctggat
     3061 tatggattgg gcaatgaacc ggttgagtgg aagaccctcc aacaaagcaa atccgctttt
     3121 ccgcaggctg gtgagctttt tgcctggtat atggatgatc ttatccggga taatatctcc
     3181 ggaccactca ctctccgtct gcgcgtgaac agcacggaag acacccatgc tgatttaaaa
     3241 attaccctta acctgagttt gccgacaccg accccaaccg cgacgccgac cccgaccgtg
     3301 accattttgc ctactgtgat cccgaccgcc acacctacgc caacgattat gatcacccgc
     3361 gagccgcgtg agaccgagac acctactccc acttcgctgc cagatgaaac gactcccatc
     3421 gtgccaaccc cgtaatcttc tggagccgga atcaccacag ggaaatctta ccctcaagat
     3481 tgccctgttt tcttt
//
LOCUS       DATMXK010000290         4524 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_53371, whole genome shotgun sequence.
ACCESSION   DATMXK010000290 DATMXK010000000
VERSION     DATMXK010000290.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4524)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4524)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4524
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53371"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VN363_05345"
     CDS             <1..460
                     /locus_tag="VN363_05345"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HWQ83968.1"
                     /translation="DVILAADAFKAAMQPLEPRLKEGTDGRRGGKFVIGVVEGDMHDL
                     GKSLVGAMLTSAGFEVIDLGIDVPAGRFIEAVKEHQPIIVGMGAYMTTTMLQMRNIIS
                     ELEAQGLRKSLKVMVGGVPTSQEFADEVGADAWGKDALDSMQKALKLIGA"
     gene            548..1774
                     /locus_tag="VN363_05350"
     CDS             548..1774
                     /locus_tag="VN363_05350"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWQ83969.1"
                     /translation="MFATMLGRHGDTIPFMGPQIHDHAMTIARVPARKYYFDAGLLVD
                     VQIAVERWYGFDTYTIIPDAYNFEVEALGAKMIYSDDAMPTVDTNVPLIKERADLEKL
                     GSLDPSKGRIPMGVELARLVSEKASGPFAMGFFCSPFSLICQAMGYPKAVRALKRDPA
                     FAQELFDFAENRAIFPYLKAQSEQPGVKQSAGADAWAAFPNLTPELVQAWVTPSAKRL
                     AERGKKELKMTVMAGLAAADYCEEDPAKFDKEIMFKCWSVARNSFVMDVALSAMGRTQ
                     DWDMHWLQEFAVQHSKLGRKLPIYASLNGRFMRDSSPQQIVEKIKEWIDIMGRDGRLL
                     FFIANVPADTPPVNVHTAVHTVHTLGRYPIAKDLASIRSAVPTFQPFDQWLKDQPEAD
                     TIFKAREWQPEKKVYA"
     gene            1804..2625
                     /locus_tag="VN363_05355"
     CDS             1804..2625
                     /locus_tag="VN363_05355"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWQ83970.1"
                     /translation="MEIEPGGPIRIVGERINPSGRSKLRQALLDENWDYIIAEARCQV
                     AAGADLIDVNVGGKGIDEVVALPQAVRVVSEAVDVPLAIDTRIPAALEAALAVCPGRP
                     LVNSIGGEKKMLAENLPIVAEHGLPVIVLCMGQEGIPKEAEGRLMVARQVLEEALRAG
                     IQEEDVIFDPLVLTVGADDQAACVALETIRLLRQEFPHNNITGGASNVSFGMPDRSSI
                     NASFLAVACTMGMNIPITDPTNPQLRSAHLAANIFLGRDQRTRQYIRHFRESKGQ"
     gene            2809..3303
                     /locus_tag="VN363_05360"
     CDS             2809..3303
                     /locus_tag="VN363_05360"
                     /inference="COORDINATES: protein motif:HMM:NF024212.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWQ83971.1"
                     /translation="MDSDTKAVAEKIIEVIPMVMQKVASELRRTGYDLAPGHYRILAI
                     LASGAHSLSELAEKQAVSLPTMSNSISILANRGWVKRERSRLDHRTVFIEVTPAGREV
                     LFAVRQSAEANVARFVACLSKEQCDQLRIGLKILESIFNRTQPSNSEQIVEELRLIKD
                     GREE"
     gene            4034..>4524
                     /locus_tag="VN363_05365"
     CDS             4034..>4524
                     /locus_tag="VN363_05365"
                     /inference="COORDINATES: protein motif:HMM:NF012791.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0032559 - adenyl ribonucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="universal stress protein"
                     /protein_id="HWQ83972.1"
                     /translation="MHNQVQRIMLAVDGSSDSQRAAKYLAGFSLPDPVSVQVSHVLPP
                     PPLDEFMIKTGFQSFYPAVSIPWDEIAEQSTHQMQEEKSTGEKLLANMVASLQASRVS
                     ASRVLLRGDAATELLKYTDENGIDLLVVGSRGLGPVKSWLLGSVSRKLVHHAGCSVLV
                     VKGG"
BASE COUNT         1099 a         1100 c         1291 g         1034 t
ORIGIN      
        1 agatgttatc ctggccgcgg atgccttcaa ggcagccatg caacccctgg aaccccgcct
       61 gaaagagggg accgatgggc gaagaggagg gaagttcgtg atcggcgtgg ttgaggggga
      121 tatgcacgat ttgggcaaga gtctggtggg cgccatgctc acttctgccg gttttgaagt
      181 gatcgacctg gggattgacg tccctgccgg tcgtttcatc gaagccgtta aagagcacca
      241 gcccattatt gtcggcatgg gcgcttatat gaccaccacc atgttacaga tgaggaatat
      301 tatttcagaa ctggaagccc aggggttacg caagagtttg aaggtcatgg ttggcggtgt
      361 ccccaccagc caggaatttg ccgacgaagt cggcgcagat gcctggggca aagatgccct
      421 ggatagcatg caaaaagcgc ttaaactgat cggagcgtga acaatggcta atccaacttt
      481 agaattgctt caaggtttgc tttatggcaa tggtttgcac attggcaagt tgagtcccac
      541 cgggcgagtg tttgccacga tgctcggcag gcacggggac accattccat tcatggggcc
      601 ccagatccac gaccacgcca tgaccattgc cagggtcccg gcgcgtaagt attatttcga
      661 tgccgggtta ttagttgacg tgcaaatagc agtagaacgc tggtatggct ttgacactta
      721 caccatcatc cctgacgcct ataactttga agtcgaagcc ctgggcgcca aaatgatcta
      781 cagcgacgac gccatgccga ccgtcgacac caatgtgccg ctgatcaaag aacgcgcaga
      841 cctggaaaaa ctcggttcac ttgacccatc gaaaggacgg attccgatgg gtgtggaact
      901 ggcccgtttg gtgagcgaaa aggcctccgg tccatttgcg atgggctttt tctgctcgcc
      961 cttcagcctg atttgccagg cgatggggta tcctaaagcg gtccgcgcgc tgaagcgcga
     1021 tccggcattt gcccaggagt tatttgactt tgccgaaaac cgggcgattt tcccttacct
     1081 gaaagcgcaa tcggagcagc ccggggtgaa gcaatcggct ggagcggatg cctgggccgc
     1141 atttccaaat ctgacaccgg aattggttca agcatgggtg actccttcgg caaaacgcct
     1201 ggcggagcgg ggcaagaaag aactgaagat gacggtaatg gcaggcctgg ctgcggcgga
     1261 ttattgtgaa gaagatccgg ccaagtttga taaggaaatc atgttcaagt gctggtctgt
     1321 ggcgcgcaat tccttcgtga tggacgtggc tttgtctgca atgggccgca ctcaggattg
     1381 ggacatgcat tggctgcaag agtttgctgt ccagcatagc aaacttggtc gcaagctgcc
     1441 catttacgcc tctctgaatg gacgctttat gcgagatagc tcacctcaac aaatcgtaga
     1501 aaaaatcaag gagtggattg atatcatggg gcgagatgga cgcctgttgt tcttcatcgc
     1561 caatgtcccg gcagatacgc cgccagtgaa tgtgcatacc gccgttcaca ccgtccacac
     1621 gctgggcagg tacccgatcg cgaaagacct ggcgtcgatc aggagcgcgg tgccaacttt
     1681 tcaacccttt gaccaatggt tgaaggacca gccggaagcg gataccatct tcaaagcgcg
     1741 cgaatggcaa cctgagaaga aagtatacgc atgacgaccc tcctgaaagg aactggggct
     1801 ctaatggaga ttgagccggg cggtcctatc cggatcgttg gagagcggat caaccccagc
     1861 ggaaggagca agctccgaca ggcgttgttg gacgagaact gggattacat aattgcggaa
     1921 gctcgatgtc aggtcgcagc cggagcagat ctgatcgacg taaacgtcgg cggaaagggt
     1981 atcgatgagg ttgtagcgct gccgcaggcg gtgagggtcg tctctgaggc ggtggatgtc
     2041 ccgctggcga tcgatacgcg catcccggct gctttggagg cagccctcgc agtttgcccc
     2101 ggccgcccgc tggtcaactc gatagggggt gaaaagaaga tgttggctga gaatctgccc
     2161 attgttgctg aacacgggct gccggtgatc gttctctgta tgggtcagga agggataccg
     2221 aaggaggcgg aaggccggct gatggttgcc aggcaggtct tggaagaggc gctccgggcg
     2281 ggaatccaag aagaggacgt catcttcgac ccgctcgtgc ttacggttgg ggctgatgac
     2341 caggcagcct gcgttgcgct ggagaccatc cgcttattga ggcaggagtt cccccacaat
     2401 aacatcaccg gcggagccag caacgttagt tttggtatgc cggatcgctc gtcgatcaac
     2461 gcttcttttt tagctgtggc ttgtacaatg gggatgaata tccctattac ggatccaacg
     2521 aatccgcaat tgcggtctgc gcatttggct gccaacatat tccttgggcg cgatcaaaga
     2581 acgcggcaat acatacggca ttttcgagag agcaaaggtc aatagaaggg ggaaattgaa
     2641 accttaattt tggagaggac tgggccgtgc ccggtccttc tcttcgtttc gctatacgtt
     2701 ctcaacccct ttagcgcaaa actgttattg ccttttgcgt tatccctttt gaaatgccac
     2761 cataccggac gtacaattag cccacctaac ctttaggggg ctaagctgtt ggattctgac
     2821 acaaaagccg tcgcggaaaa gatcatcgaa gttattccga tggtcatgca gaaagttgct
     2881 tccgaactcc gtcggacggg ttacgattta gctccaggtc attatcgtat cctggcgatc
     2941 ctggccagtg gtgctcacag cttaagtgag ctggcagaaa agcaagccgt tagcttacct
     3001 accatgtcga attcgatcag cattttggca aatcgggggt gggtaaaacg tgaacgcagc
     3061 aggctggatc acagaacagt gttcatcgag gtgacacctg cgggaagaga agtgctcttc
     3121 gcagttcgac aatccgcaga ggccaatgtg gccagattcg tagcatgtct ctcaaaggaa
     3181 cagtgtgatc agctacgcat tggattaaag attctcgaat ccatattcaa ccgaacccaa
     3241 ccttcaaatt ctgaacagat tgttgaggag ctccgcttga tcaaagatgg gagagaagag
     3301 taaatactcc acttcgaggg caagagcatg aataatagag gttcgtttga aggatcaaga
     3361 aaacagtaga gcgcaaccaa ccggggttac atttatgaaa tccgcgtaag aggccaggtc
     3421 agtcccaagt tgtccgaatg gtatttcgag aatatcacca tttccaattt ggaggatggg
     3481 gcatctttat tgaccggatg gatcgctgac caatccgctt tgtctggttt gctcaacctc
     3541 atcttcaact ggaatctcga aatcatttcg cttaacaggg tagatccctt gaatgatcca
     3601 gataaaaggt aaagtcattt gaacttacac aggcggcggg tatgcacaaa tatactcaaa
     3661 aggtcttgct cgctatcgat ggatcggaat attcgaagat agcggtatct ctcctgagca
     3721 gtttacctct ttcggtagaa agtcaggtga tctgtctgtc agtgatcgat ccccttcgcc
     3781 cccaccaacg cactgtgttg gaggcagctc tggagctggc cagatcacat ttgcaagaaa
     3841 cggttcgaaa tgtcacagcc gaactgcggc agggacatcc cgcctcagaa attaccgaat
     3901 acgctggccg acaccaaatt gacttgattg tcgtggagca aaaggtctgc gagatacgct
     3961 gggcattcta gtgggaggag ttgctcaaca agtagtcgag cttgcctgct gccctgtatg
     4021 gatcgttcga gggatgcaca accaggtcca gcggatcatg ctggcagttg acggctcaag
     4081 cgacagtcag cgagcagcta aatacctggc cggtttctca ttacctgatc ctgtctcagt
     4141 tcaagttagc catgtgctcc cgccgccgcc tttggatgaa ttcatgataa agaccggttt
     4201 tcagagcttt tacccggccg tttccattcc ttgggacgag atcgcagaac aatctacgca
     4261 tcaaatgcag gaggagaaaa gtactggaga aaaactactg gcaaacatgg tggcaagtct
     4321 tcaggcttcc agggtaagcg catcccgggt tttattgaga ggggatgctg ccactgagct
     4381 gctcaagtac acggacgaaa acgggattga cctgctcgtt gtaggatcca ggggattggg
     4441 ccctgtaaaa agctggttgc ttggaagcgt ttcgaggaag ctggttcatc acgccgggtg
     4501 ttcagttctg gtcgttaaag gagg
//
LOCUS       DATMXK010000291         4876 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_49528, whole genome shotgun sequence.
ACCESSION   DATMXK010000291 DATMXK010000000
VERSION     DATMXK010000291.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4876)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4876)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4876
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49528"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..582)
                     /locus_tag="VN363_05370"
     CDS             complement(<1..582)
                     /locus_tag="VN363_05370"
                     /inference="COORDINATES: protein motif:HMM:TIGR01906.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR01906 family membrane protein"
                     /protein_id="HWQ83973.1"
                     /translation="MSESIWVRSASWLAAVLTPVIIVLAVVRLLINPALLTFEYNMPG
                     FPPDSYGFTKEDRLYWGRYAVEYLVNDAGIEYLGDLRFADGSPVYNERELSHMLDVKI
                     VVQAAIKVLLAALVVMLILGIWAWQGYWLAAFKAALVRGGWLTIFLFGGVILFVLLSF
                     GVIFVAFHEVFFAEGTWTFYYSDTLIRMFPERFW"
     gene            complement(579..2132)
                     /locus_tag="VN363_05375"
     CDS             complement(579..2132)
                     /locus_tag="VN363_05375"
                     /inference="COORDINATES: protein motif:HMM:NF037959.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="fused MFS/spermidine synthase"
                     /protein_id="HWQ83974.1"
                     /translation="MNRYLYFAVFSSGMTVLALELAASRLLGSSFGTSNLVWASIIGL
                     ILIYLTVGYFIGGRWADRSPYPRSMYSVMAWGAFTAGLIPAIARPVLRLAANAFDQLQ
                     IAVLFGSFAAMLILFLVPITLLGTMSPFAIRLAISDPAHAGQISGRIYAISTLGSFIG
                     TFLPELLLIPLIGTAYTFLAFSMFLLLVAFGGLWLSDGWKSILRLVWMPLVLIGVGAF
                     LAGAPIKDTAGQIYETESSYNYIQVLEVDGFRYLRLNEGQGIHSTWHPTELAYDGPWE
                     QFLAGPFFSPTINNPEDVKSMAIVGLAAGTSARQATAVFGPIPIDGYEIDPEIITVGQ
                     RYFDMNMPNLNAIGEDGRWGLEHSPRRYTLIAVDAYRPPYIPWHLTTQEFFQAVYDHL
                     EADGVMVINVGRAPDDRSLIDALVGTIQTVFPTVHVMDLPGTFNSILYATRQPTKVED
                     LYQNVLHLYNRGDVHPLLIRSLTRLVTNLQPTPQSQIVYTDDWAPIELMTNSMVLKFV
                     LLGDMERLK"
     gene            2328..>4876
                     /gene="alaS"
                     /locus_tag="VN363_05380"
     CDS             2328..>4876
                     /gene="alaS"
                     /locus_tag="VN363_05380"
                     /EC_number="6.1.1.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR00344.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004813 - alanine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006419 - alanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--tRNA ligase"
                     /protein_id="HWQ83975.1"
                     /translation="MIPPEFTGARIRQTFIDFFSEHRHTFVPSSSLVPGGDQTLLFTN
                     AGMVQFKDIFLGTDKRDYTRATNSQKCMRVAGKHNDLDDVGRDGTHHTFFEMLGNWSF
                     GDYYKKEAITWAWQLLTEVWNLPRESLYATCFEDEKGQIERDDEAAHWWQQQPGILPE
                     HILFFGRKDNFWEMADTGPCGPCSEIHIDRGLEFCNKQDVSGHVCQVNGDCTRFLELW
                     NLVFIQYNRLGPDTLEPLPARHVDTGMGFERIVSVLQGVDSNYKTDLLAPLLDIVQEL
                     SGQTATQRAADPIPYQVIADHTRACTFLIADGVVPGNTGRNYVCRMIIRRAARFGGKL
                     GLREPFMALVAEKVIELYGDFYPELVRNRKTILANLTQEEERFQRTVEGGMAKLETLL
                     GRMRTSGQNTLPGDQAFELYATYGLPLELTRDVAREHELAVDEDGFRAAMDAHRLASG
                     AGENFGEMGGEDAPFYRGLLIDLQKAGKLTDEGVAYNPYQWLEVEGEVLAIIRGGKTV
                     PQAQPGDKVEILLPETGFYVASGGQVADTGAIVATRAPRWEVRVDDVRKPAAGMIVHS
                     GEVVSGIPSSGDSAIARVDSARRKDIMRNHTATHLLHHELHAVLGEHARQAGSLVAPD
                     RLRFDFTHPQALTREQLEAIEAGVNQEILGNYPLQIAYKPLQTAIAEGAIALFGEKYG
                     EVVRNITIGEPEVFSNELCGGTHVDDTGDIGLFLITSESSVAAGIRRIEAVTGRGAYE
                     LVQRRFQALRAASDLLATTPGQIMEKVQSLLEDQHATHRQIAALRQKLATTAFLERLD
                     SAQIVDGVTLLTARLDEADTDTLRQIVDQFRERFPSGSVVVLASVQDGRPNL"
BASE COUNT         1139 a         1414 c         1400 g          923 t
ORIGIN      
        1 ccagaaacgt tcggggaaca tccggatcaa ggtgtcactg taataaaagg tccaggttcc
       61 ttcagcaaag aacacctcgt ggaaagccac aaagattacc ccaaaactga gcaggacgaa
      121 taggatcacg ccgccaaaca agaaaatcgt cagccagccg ccgcgtacca gggcagcctt
      181 gaaagcagct aaccagtacc cctgccaggc ccagatcccc agaatcagca tcacgaccag
      241 agcggccagt aaaaccttga tcgcagcctg gacgacaatt ttcacgtcca gcatatggct
      301 taattcgcgc tcgttgtaaa ccgggctgcc atctgcgaag cgcaaatcgc ccagatattc
      361 gatgccggcg tcattaacca ggtattccac cgcgtaccgc ccccagtaca gacggtcttc
      421 tttcgtaaat ccataggaat ccggggggaa cccgggcatg ttgtattcga aggtcagcag
      481 agccgggttg atcagcaggc gcacaactgc gaggacgatg atcaccggcg tcagcacagc
      541 cgccaaccag cttgccgacc gaacccagat tgattcgctc attttaatcg ctccatatcg
      601 cctaacaata caaatttcaa taccatgctg ttggtcatca attcaatcgg cgcccagtca
      661 tcggtatata cgatctggct ctgcggcgtg ggctgcaggt tggtgaccag tcgggtcaga
      721 gaccggatga gcagtggatg cacatcgccg cggttgtaca ggtgcagcac attctgatac
      781 agatcctcca ccttcgtcgg ttggcgggtg gcgtacagga tggaattaaa cgttccgggc
      841 aggtccatca catgcacagt tggaaagact gtctggattg taccgaccag ggcatcaatc
      901 aggctgcgat catcgggcgc gcgcccgacg ttgatcacca tcaccccatc ggcttcgaga
      961 tgatcataaa ccgcctgaaa aaattcctgc gtggtgaggt gccacgggat gtaaggtggg
     1021 cggtaggcgt cgactgcgat gagcgtatag cggcgcgggc tgtgctccaa tccccaacgc
     1081 ccatcctccc caatcgcgtt caggttgggc atgttcatat caaaataacg ctgcccgacc
     1141 gtgattatct ccgggtcaat ctcatagcca tcgatgggaa tgggaccaaa aaccgccgtc
     1201 gcctggcggg cgctggtgcc ggctgccaga ccaacgattg ccatgctctt tacatcttcg
     1261 ggattgttga tcgtggggct gaagaacggg cctgccagga actgctccca gggaccgtca
     1321 taagccagct ccgtcgggtg ccatgtcgaa tggatcccct gaccttcgtt caggcgcagg
     1381 tagcgaaagc catccacttc cagcacctgg atatagttgt aggatgattc tgtctcgtag
     1441 atttgcccgg ctgtatcttt gattggcgca ccggccagga aagcgcctac tccgatcaat
     1501 accaggggca tccacaccag gcgcaggatg cttttccatc catcggataa ccacagaccg
     1561 ccgaaagcga ccagcagcaa gaacatactg aaagccagga atgtataggc agttccaatc
     1621 aaagggatca aaagcagctc gggaaggaaa gtgccgatga acgagcccag ggtagaaatg
     1681 gcatagatcc gccccgagat ctgaccggca tgtgcagggt cgctgatcgc caggcggatg
     1741 gcgaacgggg acatggttcc gagcaaggtg atcggcacca ggaacaggat caacatcgcc
     1801 gcaaaagaac cgaacagcac cgcgatttga agctggtcga aggcgttggc agccaggcgc
     1861 agtaccgggc gggcaatcgc cgggatcaag cccgcggtaa acgcgcccca ggccatcact
     1921 gaatacatgc tgcgcgggta aggcgaacga tctgcccagc gccccccgat gaaataaccc
     1981 acggtaaggt agatcagaat caagccaatg atgctggccc acaccaggtt gctggtgcca
     2041 aaggagctgc ccaacaaacg cgaagccgcc agctccagcg ccagaacagt catacccgac
     2101 gagaacacag caaagtacag gtaacggttc atcgatgatt gcctatgcac cccgaaattg
     2161 gatcgtggga cggattataa cttagaatac taatccccac atccgccagg tttgcctccc
     2221 gaatgcgctc cttgaaatgg gcgcagccgg gtacctgttg gtataattca tcatcttaca
     2281 ggccacaccc ctggtctaaa atctaaacca aacacggatg gaagtttatg atcccaccag
     2341 aattcaccgg cgccagaatc cgccagactt ttatcgattt tttctcagaa caccgccaca
     2401 cctttgtccc gtcctcctcg ctcgtcccgg gcggcgacca gaccctcctt tttacaaatg
     2461 ccgggatggt gcagtttaaa gatatcttcc tgggaaccga caaacgcgat tacacccggg
     2521 cgacgaattc ccagaagtgc atgcgcgtgg ccggcaaaca caacgacctg gatgatgtcg
     2581 ggcgcgatgg cacccaccac accttctttg agatgctggg taactggtcg ttcggcgatt
     2641 actacaagaa agaagcgatt acatgggcct ggcagctgct gaccgaagtc tggaatctgc
     2701 cgcgggagag cctgtacgcc acctgcttcg aggacgagaa ggggcagatc gaacgcgacg
     2761 acgaggcggc gcactggtgg cagcagcagc cgggcattct gcccgaacat atcctgttct
     2821 tcgggcgcaa agataatttc tgggagatgg cggataccgg gccatgtggg ccatgttctg
     2881 agatccatat cgaccgggga cttgaattct gcaataaaca ggatgtctcc gggcatgtct
     2941 gtcaggtcaa cggcgactgc actcgctttc tcgaattgtg gaacctggta ttcatccagt
     3001 acaaccgcct gggacccgac accctggaac ctttgccggc ccggcatgta gatacgggaa
     3061 tgggcttcga gcggatcgtc tccgttttgc agggggtcga ctcgaactac aagactgatt
     3121 tgctggcgcc actgttggac atcgtgcagg agttaagcgg tcagacggcc acacagcgcg
     3181 ccgccgaccc catcccttac caggtgatcg ctgaccatac ccgggcgtgc acattcttga
     3241 ttgccgatgg cgtggtaccg ggtaacacag gccgcaatta cgtctgccgg atgatcattc
     3301 ggcgggcggc gcgctttggc ggcaaactcg gcttacgcga gcccttcatg gcgctggtcg
     3361 ctgagaaagt catcgagctg tatggcgatt tctaccccga gctggtccgc aaccgcaaga
     3421 cgatcctggc caacctgacc caggaagaag aacgcttcca gcgcactgtg gaaggtggta
     3481 tggccaagct ggaaacgctg ctgggacgca tgcggacttc cggtcaaaat accctgcccg
     3541 gcgaccaggc ttttgagctg tatgccactt acgggctgcc gctggaatta acccgggatg
     3601 tagcccgcga gcatgaatta gcggtcgacg aagacggctt tcgagcagca atggacgccc
     3661 accgcctggc atccggtgct ggtgaaaact tcggcgagat gggcggcgaa gatgccccct
     3721 tctaccgtgg tcttttgatt gacctgcaga aggctggcaa gctgacggat gaaggcgtcg
     3781 cctacaaccc ttatcagtgg ctggaagtgg aaggcgaagt tctggcgatc atcagaggtg
     3841 gaaaaaccgt cccccaggct cagcccggag ataaagttga gatcttactg ccagaaaccg
     3901 gcttttatgt ggcttcgggc ggacaggtag ccgatactgg cgcaatcgtc gcgacccgcg
     3961 caccgcgttg ggaagtaagg gtggacgatg tgcgcaagcc agccgcgggc atgatcgtcc
     4021 acagcggcga agtggtcagc ggcataccca gcagcggcga ttctgcgatc gcccgggtgg
     4081 actcggcgcg acgcaaagat atcatgcgca accacactgc cacccatctc ctgcatcacg
     4141 agctgcatgc tgtgctgggc gagcacgccc gccaggcagg ctcactggtc gcgcccgacc
     4201 ggctgcgctt cgactttacc catccccagg cgctgacccg cgagcagctg gaagccatcg
     4261 aggccggggt taatcaagaa attttgggca attatccgct tcaaatagcc tacaagccgc
     4321 tccagaccgc catcgccgaa ggggccatag ccttatttgg cgagaaatac ggcgaagtgg
     4381 tgcgcaacat cacgatcggc gagccggagg tcttttcgaa cgagctgtgc ggcggcacgc
     4441 atgtcgatga taccggggat atcggcctgt tcctgatcac cagcgagagc agtgtggcag
     4501 ccggcatccg ccggatcgaa gccgtgaccg gacgtggcgc gtatgagctg gttcagcggc
     4561 gattccaggc tttacgggca gcgtctgatc ttctggcgac gacccccggg caaatcatgg
     4621 agaaggtcca gagcctgctg gaagaccagc atgccaccca caggcaaatc gcagccttgc
     4681 ggcagaaact ggccacgacg gctttcctgg agcgtctcga cagcgctcaa attgtcgatg
     4741 gggtcacctt gctgacagcc aggctggatg aagctgacac cgataccctg cgccagatag
     4801 tggatcaatt ccgggagcgc ttcccatccg gcagtgtggt ggtgctggcc tccgtacagg
     4861 atgggcggcc caatct
//
LOCUS       DATMXK010000292         5226 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_66464, whole genome shotgun sequence.
ACCESSION   DATMXK010000292 DATMXK010000000
VERSION     DATMXK010000292.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5226)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5226)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5226
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66464"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3904
                     /gene="dnaE"
                     /locus_tag="VN363_05385"
     CDS             <1..3904
                     /gene="dnaE"
                     /locus_tag="VN363_05385"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004226.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA polymerase III subunit alpha"
                     /protein_id="HWQ83976.1"
                     /translation="HGTMFGVIDFYNKATHAGIKPIIGVEAYLAARTMKDRDPQLDKR
                     SSHLLLLAENETGYKNLLQIASAAQLDGFYYFPRVDHDFLAQHAEGLICTSGCMSAEV
                     PRLIQNENLDAARKSLDWYFEVFGPDRFFLELQEHDIKALDRINKQLLDLGHRYDARF
                     VATNDVHYVDQEDNRYQDIMLAIQTGTLLSDPNRMRMTDPSYYLRSPQEMGRLFAEIP
                     EALSNTLLIANRCNVDLSFKGYHLPDFQVPEGCTAELYLRDLCETGLERRLGERSNDP
                     VYRERLEYELKIIHQMGFDTYFLIVWDLCRYAREQGIWYNARGSAAGSIVAYSLDITI
                     VDPVEHKLIFERFLNPGRISMPDIDLDFPDDRRSEMLQYTANRYGDDKVAQIITFGTL
                     GARAAIRDVGRVMDIPLSEVDRVAKTIPNIPGKPLKIDEALEEITELKALYNSAPYLR
                     DLIDTAKHVEGVVRHAGTHAAGLIITDKPTIEYIPLHRPTGAAAEDNPVKTVTQFEMQ
                     IIESQGLLKVDFLGLATLTIMARASNLIHERHAVDFNLSNIPIDDPATFELLGRGETA
                     GVFQVEGSGMRRWLMQMKPKNLANVIAMVALFRPGPMDFIPGYIRRMHGEEEVSYKHP
                     ALEEIFQETYGYPVYQEQLMFAAMQLAGYTAPESDDLRKAISKKQKDKLEKHREKFVK
                     GAVANGILEQTARDIFVEWEEFARYGFNKAHAADYGVIAVQTGYLKTHYPVEYMAALL
                     TVNQSDTDKVALYVADCRRMGISVEPPDINCSGWDFTIEDHADGKSAIRFGLGAIKNV
                     GHGPVKTILDERVQGPFSDLNDFVRRVDLRHVGRRALECLVKVGALDSFGSRPALLES
                     LDRITAVSAARFRAKDSGQLSLFGAHTGVVEELVLPSLNSEISRREILNWERELIGLY
                     VSDHPLSPIMAELNQAVTHYSGQLSEAAQDEKVRVAGLIVRVRHHQSKAGKPMGFVTI
                     EDLQGTIDLVVFPRTWAEYSEIIDYDRIILVDGRVDAQGAEAKVLVDRITTEFSMVSP
                     LEPVPAPSSPGRGVSLPPEEDLEETTLVSDSAAAYESNASYSAAASLNPLPTQSGEEW
                     DGDGPPPPDAFAPGLEYGGYRADLDLDAAVSKPPAPAASQMGKIQAAEISEPTEIQET
                     EGERFIQPAEEILAAQAQVAEVTEDAVPPVTPAQPDILSRTLESIPFTREEPASRPAY
                     LVSPVKPQAEGNVRMLTIVLRSTGDKTRDVLRLRRIHGIVTSYPGNDRFAFRVFERNR
                     GYLLEFPNRTIGICAELSSRLSALLGPEQISIESITFL"
     gene            complement(3990..5192)
                     /locus_tag="VN363_05390"
     CDS             complement(3990..5192)
                     /locus_tag="VN363_05390"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWQ83977.1"
                     /translation="MANVNKIKGRIGILTGGGDVPGLNPAIRAVTIRALREGYQVIGL
                     RRGWAGIIEVVRDPKADNSRNCQILSEEVVNKAGRTGGTFLHTSRTRPTSVSKNSVPE
                     HLQDTYNQEKNDMTPEVIKNLDFLGIDYLIPIGGDDTLSYGVHLYKQGLKVVAIPKTM
                     DNDVPGTDYCIGFSTCVTRTIQMTNNLRTIAGSHERILVLEVFGRYAGFTAMLPTMAG
                     AANRCVIPEHKFNIDLLTELLVYDREHNPSHYSVVLVSEGAMFEGGEMVFQDKSTDAY
                     GHAKLGGIGDLVSEELKVRSAKYNNGIQVNTINQKLGYMVRGGDPDAIDSIVPMAYGN
                     LALDMILQGIHGRLVVLQNGRYDNMPLDVVTSTKKTVNVTQHYNTERLRPFYKSFQMK
                     PLFIMTSE"
BASE COUNT         1186 a         1372 c         1431 g         1237 t
ORIGIN      
        1 tcacggaacc atgtttgggg tgatcgattt ttataataaa gctacccacg ccgggatcaa
       61 gccaatcatc ggcgtcgagg cttatctggc ggcgcggaca atgaaagatc gcgatccgca
      121 gctggataaa cgctcgtcac acttgctcct gttggcggag aacgagactg gctataaaaa
      181 tttgctgcaa attgccagcg cagcccagct ggacggtttt tactactttc cccgtgttga
      241 tcacgatttc ctggcccagc atgctgaagg gcttatctgc acttctggct gtatgtctgc
      301 tgaagtgccg cgcctgatcc agaatgaaaa cctggacgcg gctcgaaaat ccctggactg
      361 gtatttcgaa gtctttggtc cggatcgatt tttcctcgag ctccaggagc atgatatcaa
      421 agctctggat cgcatcaaca aacagctgct cgatttgggc caccgctatg atgcccgctt
      481 cgtggctacc aatgatgtgc actatgtaga tcaggaagac aatcgctacc aggatattat
      541 gttagcgatc cagaccggta ccttactcag cgatcccaac cgcatgcgga tgaccgatcc
      601 atcgtattac ttgcgcagcc cgcaagaaat gggccggttg tttgccgaaa ttcccgaagc
      661 attaagcaac accctgctca ttgcgaatcg ctgcaatgtc gacctgagtt ttaagggcta
      721 tcacctgccc gatttccagg tacccgaagg atgtacagca gagctctacc tgcgcgacct
      781 gtgcgagacg ggcctggaac gccgtttagg ggagcgctcc aatgatccgg tttatcggga
      841 gcgcctggaa tatgagttaa agatcatcca ccagatgggt tttgacacct attttctgat
      901 tgtgtgggat ctctgccggt atgctcgcga acagggaatc tggtataacg cccgcggctc
      961 cgcggccggg tcgatcgttg cctattcgtt ggatatcacc attgtcgatc cggttgaaca
     1021 caagttaatc ttcgagcgct tcctgaatcc ggggcggata tccatgcctg atattgatct
     1081 ggatttcccc gacgaccggc gcagtgagat gctgcagtac acggccaacc ggtatggcga
     1141 tgataaggtc gcccagatta tcacttttgg tacgctgggt gcgcgggctg ccatccgcga
     1201 tgtggggcgg gtcatggata tcccgctgag cgaagtggac cgggttgcta aaaccatccc
     1261 gaatatccca ggtaagccgc tcaagattga cgaagccctg gaagaaatta ctgaactcaa
     1321 agcgctgtat aactcagctc cctatctgcg tgatttaatc gataccgcca aacatgtgga
     1381 aggtgtggtt cgccatgccg gtacgcatgc tgccggattg attattacgg acaagcccac
     1441 catcgagtat attccgctcc atcgcccaac gggagccgcg gcagaagaca acccggtgaa
     1501 gacggttacc cagttcgaga tgcagatcat tgaatcgcag gggctgctca aggtagattt
     1561 cctgggtctg gcgactttga ccatcatggc gcgagccagc aatctgatcc atgaacgcca
     1621 tgctgtcgat tttaacttga gcaatatccc cattgatgat ccggctacct ttgagcttct
     1681 gggacggggg gaaactgcag gtgtctttca ggtagaaggc tcgggcatgc gtcgctggct
     1741 gatgcaaatg aagcctaaaa atttagccaa tgtcatcgcg atggtcgccc tcttccgccc
     1801 gggacccatg gatttcatcc ccggctatat tcgccggatg catggtgagg aagaagtcag
     1861 ctataaacat cctgccctgg aggaaatctt ccaggaaacc tatggatacc cggtgtacca
     1921 ggaacagctt atgttcgcgg ccatgcagct ggccgggtac acagctcccg aatcggatga
     1981 tttacgcaag gcgatctcaa aaaagcagaa agataaactg gaaaaacacc gcgaaaaatt
     2041 tgtaaaaggc gcggtcgcga atggcatcct ggaacagact gcccgggata tcttcgtcga
     2101 atgggaagaa tttgcccggt atggtttcaa taaagcccat gctgccgatt atggggtcat
     2161 cgcagttcaa actggctatc taaaaaccca ttatccggtt gaatatatgg ccgccctgct
     2221 gacggtcaac cagagtgata ccgataaagt tgccctgtat gttgccgact gccgccggat
     2281 ggggatttcc gtcgaaccac ccgatatcaa ctgcagtggc tgggatttta caatcgaaga
     2341 ccatgcagat ggcaaaagcg ccattcgctt tggtctgggg gcgatcaaaa atgtcggtca
     2401 tggaccggtc aaaacgatcc tggatgaacg cgtccagggc ccgttttccg atctgaatga
     2461 ttttgtcagg cgggtcgatt tacgccatgt gggcaggcgg gcgctggaat gcctggtcaa
     2521 agttggggcg ctggattctt tcggcagccg ccccgccttg cttgaatccc tggatcgcat
     2581 cactgctgtc tcagctgccc gctttcgggc aaaagattcg ggccagctca gtttgtttgg
     2641 cgcccatacc ggggtggtgg aggagctcgt tctgccgtct ttgaacagcg agatcagccg
     2701 ccgcgaaatc ctcaactggg agcgggagct gattgggctg tatgtctccg atcatcccct
     2761 cagtccgatt atggcggaat tgaaccaggc ggttacccat tattcaggcc agctttcgga
     2821 ggctgcgcag gatgaaaagg tgcgggttgc tggtttgatt gtgcgggttc gtcatcacca
     2881 gagcaaggcc ggcaaaccga tgggctttgt caccatcgag gatttacaag ggacgatcga
     2941 tctggtcgta ttcccgcgca cctgggctga atacagcgaa atcattgatt atgatcgcat
     3001 tatattggtg gatggcagag tggatgcgca aggtgcagag gcgaaggtcc tggtggatcg
     3061 tataaccaca gaattctcca tggtctcgcc acttgagcca gtccccgccc caagctctcc
     3121 tgggcgaggg gtctcccttc ctcctgaaga agatctggaa gagacaactc ttgtatctga
     3181 ctccgcagct gcgtatgaat ccaatgcctc ctactcggcg gccgcgtctt tgaatccact
     3241 tccaacgcaa tctggtgagg agtgggatgg agatggccca ccgcctccgg atgcatttgc
     3301 tcctggcctg gaatatggcg gctatcgggc tgatctggac ctggatgcag cagtcagcaa
     3361 gcccccagcg ccagcagcca gtcagatggg aaaaatccag gcagcggaaa tttctgaacc
     3421 aactgaaatt caagaaactg agggcgagcg ttttatccag ccagccgaag agattctggc
     3481 tgcccaggcg caagtggctg aggtgactga ggatgcagtc ccgccagtga caccagccca
     3541 gccggatatc ttgtccagga cgcttgaaag catacccttc acccgcgaag agccggcctc
     3601 ccggcctgct tacctggtct caccggtcaa acctcaagct gaggggaatg tgcgcatgct
     3661 gaccatcgtg ctgcgctcta ccggcgataa gacgcgggat gtcttaagat tacgccgtat
     3721 tcatgggatc gtgaccagtt acccgggcaa tgacaggttt gcctttcggg ttttcgagcg
     3781 caatcgcggc tatttgttgg agtttccgaa ccggactatc gggatttgcg ccgagctgag
     3841 ctcacgcctg agcgctctgc tcgggccgga gcagatcagc atcgaatcga tcacattcct
     3901 ataaaaatca gtccctgaat aaaaacatcc gccggaggaa aattgctgcc tccggcggtt
     3961 tcggttaata caggggtttt ctgcctggat tattctgaag tcatgatgaa cagcggtttc
     4021 atttgaaagc ttttatagaa tgggcgcagg cgctctgtgt tgtaatgctg ggttacattg
     4081 acggttttct ttgtggaagt gacaacatcc agcggcatat tatcatagcg cccattttgg
     4141 aggactacca gccggccgtg gataccctgt aagatcatat ccagggccag gttgccataa
     4201 gccatcggta cgatcgagtc gatcgcatcc gggtcgcccc cgcgtaccat atatcccagc
     4261 ttctgattga tcgtattgac ctggatccca ttgttatatt ttgccgagcg caccttaagc
     4321 tcttctgaaa ccagatcgcc aattccgccc aacttggcat gtccgtatgc atcggtggat
     4381 ttgtcctgga agaccatttc gccgccctcg aacatggcgc cttccgagac caggacgact
     4441 gaataatggc ttggattgtg ctcgcggtcg tagaccaata actctgtcaa aagatcgata
     4501 ttgaatttat gctcgggaat tacacagcgg ttggctgcac ctgccatggt cggcagcatg
     4561 gcggtgaacc cggcatagcg gccgaagact tccaacacca ggatgcgctc gtgcgagccg
     4621 gcaatcgtgc gcaggttgtt ggtcatctgg atggtgcgtg taacacacgt gctgaagccg
     4681 atgcaataat ccgtaccggg cacgtcgttg tccattgttt tggggatggc tacgactttt
     4741 aagccttgct tgtataggtg aaccccgtag ctcagcgtgt catcgccgcc gatcgggatc
     4801 aggtaatcga tacccagaaa atccaggttc ttaatgacct cgggggtcat atcatttttt
     4861 tcctggttat aagtgtcctg caggtgttca ggtacactat tttttgaaac actcgtaggc
     4921 cgggtgcggg aagtatgcag gaaagtaccc ccggttcgac cggctttatt gaccacttcc
     4981 tcgctcagga tctggcagtt ccggctgttg tcggctttgg ggtcgcggac aacttcgatg
     5041 atgccagccc agccacgccg caagccaatc acctgataac cctcacgcaa ggcacggatg
     5101 gttacagccc ggatggcagg gttcaggcca ggtacgtcgc ctccgccggt caggatgccg
     5161 atgcggcctt tgattttatt cacatttgcc atttaattac ctccaaaaaa ataaaaatgc
     5221 ataaaa
//
LOCUS       DATMXK010000293         5612 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_116309, whole genome shotgun sequence.
ACCESSION   DATMXK010000293 DATMXK010000000
VERSION     DATMXK010000293.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5612)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5612)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5612
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116309"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..338
                     /locus_tag="VN363_05395"
     CDS             <1..338
                     /locus_tag="VN363_05395"
                     /inference="COORDINATES: protein motif:HMM:NF027546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="winged helix-turn-helix domain-containing
                     protein"
                     /protein_id="HWQ83978.1"
                     /translation="PAIITETRLAASRDVILGLIDAWEHNRYVINRFKGEVFCSGYGI
                     WIDYRTNPEGHRRLFDIMERCDGEHTIADIAAELQIPFQAVLEVAQLLESKELVRLSW
                     TPESTDPHR"
     gene            363..1226
                     /locus_tag="VN363_05400"
     CDS             363..1226
                     /locus_tag="VN363_05400"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWQ83979.1"
                     /translation="MNIPPSFKHSLKSVRYQGRRWAVALRYGAGTLDKLPAVLGNAMP
                     KSGSHLIIQVLQGLTRIGPFVNPGYPPVNRGEDNRKLSDQQVLENILRMQPGEISYGY
                     ISAKEPFLSVLTQSGRATIFVYRDPRDMIISHIFYATQMHAGHWMHSYYTEKLNSMEA
                     RIQAAIQGVEEPGSELTPIRQRYEGYLGWLEQPQVLSLRFEELILERELAFGKLLEYF
                     AGFGFRSRLPREEAIAILQEAIAPRKSGTFRKGKPGNWREHFTPDNIATFKAATGDLL
                     VRLGYETSLDW"
     gene            1232..1864
                     /locus_tag="VN363_05405"
     CDS             1232..1864
                     /locus_tag="VN363_05405"
                     /inference="COORDINATES: protein motif:HMM:NF014069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWQ83980.1"
                     /translation="MQVEYRLKVPTLQQARTMLDEAAGMNPGPWVAHSQITARAARLI
                     AAELSELDPEAAYILGCLHDIGRREGVHGMRHAIDGYQFLAAQGFEDAARICLTHSFP
                     IPIAAAGSDWDGTPADFDFLQDYLDHLEYDIYDRLIQMCDSISQPTGYCLMEKRLVDV
                     ALRYGFNPHTRAKWQAFIGIRQDFELKLGKSIYSLLPGIVENTFESQSLP"
     gene            1842..2753
                     /locus_tag="VN363_05410"
     CDS             1842..2753
                     /locus_tag="VN363_05410"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWQ83981.1"
                     /translation="MKARASHNTMLGYPSSLRFQINSGGRLVRRSARRLAQALRYRQF
                     SFSGIPVLFANSFPKSGTHLLTQILAGFTQIGPAVDSGLPAVVTYDGFTGLQRPPDEI
                     LADLERLLPGDIAYGHVHALPEIIDFLARAPFAFYFILRDPRDVVVSHVHYVTDMEPN
                     HIHHPYYKDELTSFAERLKVSIRGRPELAIDFPNIRQRFDPFMAWLDQPNVLTIHYED
                     FILNRQAALGQVLDFAVRRGFPLQVGRQAALETLESSIDPQRSPTFRSGKTGGWKDQF
                     TEAHKELFKAVTGDLLIRLGYEQSHDW"
     gene            2743..3741
                     /locus_tag="VN363_05415"
     CDS             2743..3741
                     /locus_tag="VN363_05415"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560175.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ83982.1"
                     /translation="MIGDRSLPRASIVIRAYNEARHIERLLTGICQQALNGKPVQDIE
                     VIVVDSGSTDGTVERARLFAAPFPIRIVHIPPEEFTFGRSLNLGIQASRSPLIVIASA
                     HVYPVYPDWLERLLAPFSDDQVVLSYGKQRGNHLSHFSEQQIFSTWYPDQTQPRQSHP
                     FCNNANAAIRRGIWEEHPYDESLSGLEDVEWANWAVEKGYAISYCADAVVLHIHRETP
                     QSVFNRYRREAIAFKRIFPHQRFGKIDLGRLVSRNILSDLRQAARQKVLLHSIGSIFW
                     FRWMQFWGTYKGYRHSGPLTWQLRQTFYYPFDPNQAQITLSRAVEPIPYPDLEKDT"
     gene            3747..4433
                     /locus_tag="VN363_05420"
     CDS             3747..4433
                     /locus_tag="VN363_05420"
                     /EC_number="2.7.7.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559078.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016779 - nucleotidyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase family
                     protein"
                     /protein_id="HWQ83983.1"
                     /translation="MSDTSPRIAALIPMRHHSQRVPGKNFRPLAGKPLYQHILDNLAA
                     CPEITTIAVDTDSLEIISGLESHYPQVQIIPRPEHLRADQVPMNEILAYDTGLVEADF
                     YLQTHSTNPLLRSETISRAIQQFLASYPAYDSLFGVTRLQTRLWDQLGRAINHNPAVL
                     LQTQDLPPVYEENSCLYIFTRQTLLSRRNRLGERPAMFEINPSEAWDIDEELDFQITG
                     YLLQIQNQAK"
     gene            4460..5407
                     /locus_tag="VN363_05425"
     CDS             4460..5407
                     /locus_tag="VN363_05425"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphoglycerate dehydrogenase"
                     /protein_id="HWQ83984.1"
                     /translation="MPAVLMSAPYMIPFMDRFTPVFQRHGLEVIVAPVRERLEEDQIL
                     AFAGQFDGAVCGDDRYTARVLEACTPRLKVISKWGTGIDSIDQAACAQLGIQIYRTPN
                     AFTLPVGDTVMGYLLAFARRQPWMDRAMKAGEWEKLPSRSLSECTLGVVGVGNCGKAV
                     LRRAHAFGMKLLGNDIVEIAPDFIAEYGVEMTSLEELLSRADFISLNPDLNSTSYHLI
                     NARTLSQMKPTAILVNTSRGPIVDEPALIAALQNKQIAGAALDVFEVEPLPAGNPLKR
                     MDNVLLAPHNANSSPAAWERVHWNTIRNLITGLGIPFQN"
     gene            5444..>5612
                     /locus_tag="VN363_05430"
     CDS             5444..>5612
                     /locus_tag="VN363_05430"
                     /inference="COORDINATES: protein motif:HMM:NF013530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ83985.1"
                     /translation="MQTSSNSSTQRTALVTGAAGGIGSATVLKFIEAGWQVIAVDRAE
                     KPDRFTAAVSYI"
BASE COUNT         1225 a         1627 c         1459 g         1301 t
ORIGIN      
        1 ctccggcgat catcactgaa acccggctgg cggcctcgcg ggatgtaata ttagggttga
       61 tcgatgcctg ggaacacaac cggtatgtta tcaaccggtt taaaggcgaa gttttctgct
      121 ctggttacgg catctggatc gattaccgca ccaacccgga aggccatcgc cgcctttttg
      181 atatcatgga gcgctgcgat ggtgagcata cgattgccga tatcgccgcc gagctgcaga
      241 tcccctttca ggctgtttta gaagttgccc agctccttga atccaaagaa ttggtccgtc
      301 tgagctggac tccggaatct accgatccac atcgctaaac agccacttca gcaggtaatt
      361 ccttgaatat tccgccttcc ttcaaacaca gcttgaaatc ggtccgctac cagggcaggc
      421 gttgggcggt tgctttacgc tacggcgccg gcactctgga taagctgcct gcggtattgg
      481 gtaacgccat gcccaaatct ggctcgcatt tgattatcca ggtgcttcaa gggctgacca
      541 ggatcggtcc gtttgtcaat ccaggctacc cgccagtcaa ccgcggcgag gataatcgca
      601 agttgagcga tcagcaggtg ctcgaaaaca tcctgcgcat gcagcctggc gagatcagct
      661 atggctatat cagtgccaag gagccgtttt taagcgttct cacccaatcc ggccgggcga
      721 cgatcttcgt ctatcgcgac ccacgcgata tgatcatctc ccacattttt tacgccaccc
      781 agatgcacgc cggtcactgg atgcacagct actacaccga aaagctaaac tcaatggaag
      841 cgcgtataca ggctgccatc cagggtgtcg aggaacccgg atcggagctg acccccatcc
      901 gccaacgtta tgaggggtat cttggttggc tggagcagcc ccaggtgctc agcttacgct
      961 ttgaagagct gatcctggag cgtgagctgg cctttggtaa gctgctggaa tactttgctg
     1021 gctttggatt tagatcccga cttccccgag aagaggccat cgcgatcctt caagaggcga
     1081 ttgcgccgcg caaatcagga acattccgta aaggcaagcc gggtaactgg cgcgaacact
     1141 tcacacctga caatattgcc actttcaaag cagccactgg cgatctgctg gtccgcctgg
     1201 gttatgagac cagcctggat tggtgagcca gatgcaggtc gagtacaggc tgaaggtccc
     1261 taccctacaa caggccagaa cgatgctgga cgaagccgcc gggatgaatc cgggaccgtg
     1321 ggttgctcat tcccagataa ctgcacgggc agctcggtta attgccgccg agctgtccga
     1381 gctcgatcca gaggctgctt atatcctggg ctgcctgcac gatatcgggc ggcgggaagg
     1441 tgtgcatggc atgcgccacg ccatcgatgg ataccagttc ctggcggcgc aaggatttga
     1501 ggatgctgcc cggatctgcc tgactcactc gttcccgatc ccaattgccg ccgctggttc
     1561 ggattgggat ggcacacctg cagatttcga ttttctccag gactatctgg atcacctgga
     1621 gtatgacatc tatgaccgcc tgattcagat gtgcgactca atctctcagc caaccgggta
     1681 ctgcctgatg gaaaaacgat tggtggacgt cgccttgcgt tacggtttca acccgcatac
     1741 ccgcgccaaa tggcaggcat ttatcggtat ccgccaggat ttcgaactca aactgggcaa
     1801 atctatttat tcactcctgc ctgggatcgt cgagaatact tttgaaagcc agagcctccc
     1861 ataacaccat gctcggatat ccatcctccc tccgcttcca aattaattct ggcgggcgac
     1921 tggttcgccg ttcggctcgc cgtctggccc aggcgctgcg ttacaggcag ttctcattca
     1981 gcggtattcc ggttttattt gccaattcct tccccaagag cggtacccat ttgctcaccc
     2041 agatcctggc tggctttacc caaatcggtc ccgcagtgga cagcgggctg ccggcggttg
     2101 ttacctatga tggttttaca ggcttgcagc gtccgcccga tgagatatta gctgatctgg
     2161 agcgcctgct gccaggggat attgcctacg ggcatgtgca tgccctgccg gaaatcatcg
     2221 attttcttgc cagggcgcca tttgcctttt acttcatcct ccgcgaccca cgggatgtgg
     2281 tggtctcaca tgtccattat gtgacggata tggagcccaa ccacattcac catccctact
     2341 acaaagatga attgaccagc ttcgctgagc ggctgaaggt cagtatccgc ggccggcccg
     2401 agctagcaat cgattttccc aacatccgcc agcgattcga tcctttcatg gcctggcttg
     2461 accagcccaa tgtcttaacc atacactacg aggattttat ccttaatcgc caggcagctc
     2521 tcggtcaggt gttggatttt gcagtccgga gaggcttccc cctccaggtt ggtcgccagg
     2581 ctgccctgga aacgctggag agcagtatcg atccgcagcg ctcgcccacc ttccggagtg
     2641 gaaaaaccgg cggctggaaa gaccagttta cggaagctca caaagaactt tttaaggcag
     2701 tcacagggga tctattgatc cgcctgggtt atgaacaatc gcatgattgg tgaccgttct
     2761 ctgccgcggg cttcaattgt catccgtgcc tataacgaag cccggcatat tgaacgcctt
     2821 ctgacaggta tctgccagca ggccctgaat gggaagccgg ttcaggatat cgaagtcatc
     2881 gtggtggatt caggctctac agatggcacg gtggagcgtg cccgcctgtt tgctgctccc
     2941 ttccccattc ggatcgttca catcccgccg gaggaattta ctttcggccg ttcgctaaat
     3001 ctgggtatcc aggcttcccg cagcccgctg atcgtcattg ccagcgccca tgtttatccg
     3061 gtctatccgg attggcttga acgcctcctg gcgccattta gcgacgacca ggtagttttg
     3121 agctacggca agcagcgggg taatcatctg tcacattttt cagagcagca gattttttcc
     3181 acctggtacc ccgatcaaac tcaaccgcgc cagagccacc ctttttgcaa caatgccaat
     3241 gccgccattc ggcgcgggat ctgggaagaa catccctatg atgaaagcct ctcggggctg
     3301 gaagatgtgg aatgggcaaa ttgggccgtg gaaaaaggct atgcgatcag ttattgcgcg
     3361 gatgctgtag tcctccatat tcaccgggaa acgccccaat ccgtatttaa tcgttaccgg
     3421 cgtgaagcga tagcgttcaa gcgcattttt ccccaccagc gatttggaaa gatcgatctg
     3481 gggcggttgg tatcgcgaaa tatcctcagc gacctgcgcc aggcagccag gcagaaggtt
     3541 ttactgcaca gtattggcag catcttctgg ttccgctgga tgcagttttg ggggacctat
     3601 aaaggctatc gccatagcgg tccgctcacc tggcagctcc gccagacatt ttattatcct
     3661 tttgacccca atcaggccca gattactctg tctcgggcgg tcgaaccaat cccctatcct
     3721 gatctcgaaa aggacacctg accggaatgt ctgatacttc cccacggatt gccgcgttaa
     3781 tccccatgcg ccaccattca cagcgcgtgc ccggaaaaaa ctttcgcccc ctggcgggca
     3841 aaccccttta ccagcatata ttggataacc tggcagcctg tcccgagatt acgaccattg
     3901 cggtcgatac ggacagcctg gagatcatct ccggcctgga aagccactac cctcaggtgc
     3961 aaatcatccc ccgcccggag catttacgtg ctgaccaggt ccccatgaat gagattctgg
     4021 cttatgatac cggtctggtt gaggctgatt tttatctgca gacccacagc accaacccgc
     4081 ttctgcggtc agagacgatc tcacgggcga tccaacaatt cttagccagc tatcctgcat
     4141 atgattccct gtttggcgtt actcgtctgc agacccgcct gtgggaccag cttgggcggg
     4201 cgatcaacca caatcctgcc gttttgctgc aaacccagga tttacccccg gtgtatgaag
     4261 aaaactcatg cctgtatatc ttcacccgcc agaccctgct cagccgccgt aaccgcctgg
     4321 gtgagcgccc ggcaatgttc gagattaacc catccgaagc ctgggatatc gacgaagagc
     4381 tcgactttca gatcaccggt tacctgttac aaatccaaaa tcaagccaaa taatcaaaaa
     4441 tctccaagga acttgcttta tgcctgcagt attaatgtcc gcaccgtata tgatcccatt
     4501 catggatcgc ttcaccccgg tcttccaacg ccatgggctg gaagtcatcg tcgcaccggt
     4561 gcgtgagcgc ctggaagaag accagatcct ggcattcgcc gggcagtttg acggggcggt
     4621 gtgcggagat gaccgctaca cagctcgagt tctggaagcc tgcacaccgc gcttaaaagt
     4681 gatctcgaaa tggggaaccg ggattgactc gatcgaccag gcggcctgtg cccagcttgg
     4741 aatccaaatt tatcgcaccc cgaatgcctt cacgcttccg gttggtgata cagtcatggg
     4801 ctatctgctg gcatttgccc gccgccagcc ctggatggac cgggcaatga aagccgggga
     4861 gtgggagaag cttcccagcc gctctttgag tgagtgtacc ctgggtgtgg tcggggttgg
     4921 taactgcggt aaagcggtcc tgcgcagagc gcatgctttt ggaatgaagc tgttgggcaa
     4981 tgatattgtc gaaatagcgc ctgattttat cgctgaatac ggcgttgaaa tgacttccct
     5041 tgaggaactc ctgagccggg ctgactttat cagcctgaac ccagacctca actcaaccag
     5101 ttatcatctg ataaatgccc gcactctgag ccagatgaag ccaactgcga ttctggtcaa
     5161 tacctcccgg ggtcccatcg tggatgaacc tgctttgatc gctgcgctgc agaacaagca
     5221 aattgctggg gctgccctgg atgtgtttga agtcgaaccc ttacccgccg gtaatcccct
     5281 gaagcgcatg gataacgtgc tcctggcacc gcataatgcc aattccagcc cggctgcctg
     5341 ggaacgcgta cactggaaca ccatccgcaa tctgatcacc ggcctgggca tcccctttca
     5401 aaattaagcg taagctgcca tcaaactgct aggataaata gagatgcaaa cctcctcgaa
     5461 ttcctcaacc caacgaacag ccctggttac cggcgctgcc ggtggcattg gcagtgctac
     5521 cgttctcaaa tttatcgaag ctggctggca agtgatcgcc gtagaccgcg ctgaaaaacc
     5581 ggaccgtttc accgccgcgg tcagttacat cc
//
LOCUS       DATMXK010000294         3179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_152557, whole genome shotgun sequence.
ACCESSION   DATMXK010000294 DATMXK010000000
VERSION     DATMXK010000294.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152557"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..601
                     /locus_tag="VN363_05435"
     CDS             <1..601
                     /locus_tag="VN363_05435"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ83986.1"
                     /translation="VIAEADNGQTAEDLIQELHPDVAVLDIQMPKASGIEVTRWVRAN
                     VSGVGVLILTAYDDDPYVLAVLQAGANGYVLKTAEPEELIRAVRSVNEGKSALDATIA
                     AKLMGVLQSSGAGGRYSADKAIPVENLSGREMEVLELVARGFINKAIATQLQISDRTV
                     QGHLARIFAKLHAGSRTEAVMRAVAMGWISSDVGQITKE"
     gene            576..2351
                     /locus_tag="VN363_05440"
     CDS             576..2351
                     /locus_tag="VN363_05440"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ83987.1"
                     /translation="MWARLRKNKLPLDDRSWSGLMVQLFSFVILPMAVVLLAISFISQ
                     AMHDQSMRTMVGVRDKRAVITAATALEADIRERIATVHLLALQAESSSYEAVGKSISG
                     SQFLIDQFDGGIALINNGGELLELSAGDGSLQSLASSLEVPSQNLFSGSMTHFLSDAF
                     KSPNSGERLLVILETAPEREYLAAGAFTIRSLNPAALDSLLVDTHSGAYFVVDRQGQV
                     VLSAGHAPGQSDLSAHPGVEAALQGLSGTSFIDGGWNELVVAYSSLPILGWGLVSEED
                     WEMVSSPSLRSTQLAPLILAPFLLLMLVALGYGAQQIVGPLRKLEERAARLAWGDYKA
                     IQEPIGGLAEIRSLHGELVHMAEKVQASQQGMYDYVGAVTAAQEDERRRLAQELHDDT
                     IQSLIALKQRAQLMRMQMKNRLTEQEAADLNELLALSEQTIENLRRQIRALRPIYLED
                     LGLSTAFGMLAQEASQTAGIPVTFQLSGAERRLDESVELALYRIAQEALNNVVWHAQA
                     KSAAVELEFEAEAVRLSITDDGTGFQAPRSPAELAAQGHFGLLGISERADLIGAQLDL
                     QSTPGQGTRIQIRLDTAVEGKKTPD"
     gene            2446..3030
                     /locus_tag="VN363_05445"
     CDS             2446..3030
                     /locus_tag="VN363_05445"
                     /inference="COORDINATES: protein motif:HMM:NF019301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DoxX family protein"
                     /protein_id="HWQ83988.1"
                     /translation="MATQAISKSYIVSDPPAITDLFSNTRWSFLWLAIRLYLGYEWFT
                     HGLEKLANPAWAVTGDALKGFWSRAVQIPETGSPAIAFGWYRSFLGALLESGSYTWFA
                     KLVVAGEILIGVALILGLFTGIAAFLGGFMNWNFMMAGAASVNPVFFVLSILLLLAWK
                     TAGWIGLDRWLLPLLGVPWKPGVLFKKTSEEPAT"
BASE COUNT          669 a          851 c          976 g          683 t
ORIGIN      
        1 ggttattgct gaagcagata acgggcagac ggcagaagac ctgatccagg agctgcatcc
       61 ggatgtggca gtgttggata tccagatgcc gaaagcgagc gggattgaag tgacccgctg
      121 ggtgcgcgcc aatgtatccg gggtaggagt gctcatcctg acagcctatg acgatgatcc
      181 atacgttctg gcggtcctgc aggcaggggc gaatgggtat gtgcttaaaa ccgccgagcc
      241 agaggagctg atccgggcag tgcgaagtgt caacgaggga aaatcagccc tggatgcaac
      301 catcgccgcg aagctgatgg gtgtgctgca gagcagtggg gcaggcggga gatacagcgc
      361 tgacaaggcg atcccggttg aaaacctttc cgggcgtgag atggaggtgc tggagcttgt
      421 ggccaggggt tttatcaata aagccattgc cacccagctc cagatcagcg atcgcacggt
      481 gcaggggcac ctggcacgca tatttgccaa gctgcatgcc ggcagccgca ccgaagcggt
      541 gatgcgagct gtggcgatgg gatggatctc atctgatgtg ggccagatta cgaaagaata
      601 agcttcccct ggacgaccgc tcctggagcg ggttgatggt ccagctgttc tccttcgtca
      661 tcctgccgat ggcggtggtt ctgctggcga tcagcttcat cagccaggcg atgcatgatc
      721 agtcaatgcg caccatggtc ggtgtgcgcg ataagcgcgc cgtgatcacc gccgcgacag
      781 cactggaggc cgatatccgt gagcggatcg cgaccgttca tctgcttgcc ctgcaggctg
      841 aatccagttc ttatgaggcg gtcggaaagt ctatttccgg gtcgcaattc ctgatcgacc
      901 agtttgacgg cgggatagca ctgataaata atggaggtga gcttctggag ctgtcggcgg
      961 gggatgggag cctgcaatcg ttagcctcca gcctggaggt tcccagccag aatctgttca
     1021 gcggatctat gacccatttt ttgagcgatg cttttaaaag tccgaacagc ggcgaacgcc
     1081 tgcttgtaat ccttgaaacc gcgccggagc gggaatacct ggcagccgga gcttttacca
     1141 tccgcagcct gaacccggct gcgctggata gcctgttggt cgatacccac agtggagcgt
     1201 attttgtagt cgaccggcag ggccaggtag ttctatcggc tggtcacgcg ccggggcaaa
     1261 gcgatctttc cgcgcatccc ggagtggaag ccgcactgca gggattaagc ggcacaagct
     1321 ttatcgatgg aggctggaac gagctggtgg tggcatacag cagcctgcct atcctcggat
     1381 gggggctggt cagtgaagaa gactgggaaa tggtctcttc acccagcctg cgcagcactc
     1441 agcttgctcc cctgatcctg gcgccatttt tgctcctgat gctggtggcg ctggggtatg
     1501 gtgcgcagca aattgttggt ccgctgcgca aactggagga aagggctgcc aggctggcct
     1561 ggggagacta caaggctatt caggaaccga ttggcgggct ggctgaaatt cgcagcctgc
     1621 acggcgagct tgtgcacatg gcggaaaaag tccaggcttc tcagcagggg atgtacgatt
     1681 atgtcggggc tgtcaccgcc gctcaggagg atgagcgccg ccgcctggcg caggagctgc
     1741 acgatgatac gatccagtcg ctgatcgccc tcaagcagcg agcccaactg atgcgaatgc
     1801 agatgaaaaa ccgcctgact gaacaggagg cggcggacct caacgagctg ctggcgctga
     1861 gcgagcagac gatcgaaaac ttacgccgcc agatccgggc attacgtcct atttatctgg
     1921 aagacctggg attgagcaca gcttttggga tgctggccca ggaagccagc cagacagccg
     1981 gaataccggt cactttccag ctttcaggcg cagaacgccg gctggatgaa tcagtggagc
     2041 tggcactata ccggatagcg caggaagcct tgaataacgt cgtctggcac gcccaggcta
     2101 aatccgcggc ggttgagctg gaattcgagg ctgaggctgt ccgtctgagc atcacagatg
     2161 atgggaccgg atttcaagcg ccgcgcagcc cggctgagct ggctgctcag ggacattttg
     2221 ggctgctggg gatctccgag cgggccgatc tgattggcgc ccagctcgac ctgcagtcca
     2281 cacccggaca aggtacccgg atccagattc gcttggatac ggcagtcgag gggaaaaaaa
     2341 ctccagatta ggcgaaaatg cctaaccaat gcgcgcagaa ttgcgctttt gccaatctcc
     2401 ttatctggat aaactatacc cagatcacca aataaggaga cacttatggc aactcaagca
     2461 atatccaaaa gttatatcgt ttctgatcca cctgcaataa ccgatctttt cagcaatacg
     2521 cgttggtcat tcctgtggct ggctattcgc ctgtacctgg gctacgagtg gtttacccat
     2581 ggtttggaaa aattagcgaa tcctgcctgg gcagtgaccg gggatgcttt gaaaggcttc
     2641 tggagccggg cagttcagat ccccgaaacc ggcagcccgg cgattgcctt tggctggtac
     2701 cgctcattcc tgggggcact tctggaaagc ggcagttata cctggtttgc caagctggtc
     2761 gtggctggtg agatcctgat cggcgtggca ttgatcctgg gcttattcac cgggattgca
     2821 gccttcctgg gcggcttcat gaactggaat ttcatgatgg ccggcgccgc aagcgtgaac
     2881 ccggtatttt tcgtcctctc tatcctcctg ttattagcct ggaaaaccgc cggctggatt
     2941 ggccttgacc gctggctgct ccccttgctg ggtgtgccgt ggaagccagg cgtattgttc
     3001 aagaaaacca gcgaagagcc tgctacgtaa tcagctcaat tcgttgtctt ttctccccct
     3061 ccagcctccc gcagttccct cctgcgggag gccctttttg tcttgacgcc tgccatgccg
     3121 tgtggtaaat tcagcccagg taaacaacag ccccgtcaac acggggcttt tttgtaagc
//
LOCUS       DATMXK010000295         2773 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_24009, whole genome shotgun sequence.
ACCESSION   DATMXK010000295 DATMXK010000000
VERSION     DATMXK010000295.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2773)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2773)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2773
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24009"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(58..1590)
                     /locus_tag="VN363_05450"
     CDS             complement(58..1590)
                     /locus_tag="VN363_05450"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83989.1"
                     /translation="MKRKTTIGAAFALLILVVVVGTVGAQYAGFSWTSVYQVVNMGDA
                     PADITVSYFDGNGVQQTHQQVFTDVPSGSSRLVVQFTDDTALGSGLFSAVVAADQPVA
                     AIANLQLVPDGATSYNPAPPFGTYSAEGQGSTSVILPAVMYNWYNYYTEVYIMNVGTG
                     AASVDIEYIPGVVNGVATGTALDDLDNAVPQYASLNISQQAESGLGAPPGAYAGRFLG
                     SAILTSDQPIIAIVNQHNVNDSKLMTYNGFTDGGSLDVAAPVHMRGYYGYYSTLLVAN
                     PSTSAAAHVEITYTPDLSRPNVVSSGSIAPVTVPFTIDPQTSLTRHDGPTATDVQSDL
                     DDTNVYTRFFGSVRVTSDIPVVVLANQEALAATDAQGGSYNGAAVSTGTAEVVIPVIM
                     ADYYGYYTTTVVQNMTGTDGVCDITYTSDGTYSSVKNLSETYHHELPGNGAFTIYEGR
                     KGGVEDGDINADDQWIAGDGTRRFLGAATMTCDVDVVSFVNEEKDILLEDSMYTYNAF
                     NK"
     gene            complement(1682..2563)
                     /locus_tag="VN363_05455"
     CDS             complement(1682..2563)
                     /locus_tag="VN363_05455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83990.1"
                     /translation="MFLVLGYAIWRDWSVIRSFEWGFNPLNLLAYVIFHTISLGCMFA
                     GWHLTMKRLAGRSNLRTDFRIYSLSLLARHIPLPIWFVGSRVVLYQAEDIPPSIPLTA
                     TALEHALLAFGGVVCYLSLLPWYAYTQRIPWIIPVSLLVFFLAVFFIRPGLLIDLINK
                     IQNYRHKPNLDVVISRRDLTIWGLLYLAPWFLDGIGFYFALTTFLPLPNQVVSFIGIS
                     TVSTLVAMLTMILPAGFGLKELTMGAMLGTWLPVSAGIVLSLVYRLLITLVETFWAWF
                     GQSNTDGLPAEHSSPVN"
BASE COUNT          720 a          672 c          756 g          625 t
ORIGIN      
        1 gatctgcgtt gtacttaatc cttaagaata aggactggtt cgacagcgcc ggtcaggcta
       61 tttattgaaa gcgttgtaag tgtacatcga gtcttcgagg agaatatctt tttcctcatt
      121 gacgaaggat actacatcta catcgcaggt catcgttgct gcgccaagga agcgcctggt
      181 gccgtcaccg gcgatccatt ggtcatcggc attgatatcg ccatcctcta caccaccttt
      241 acgaccttcg tagatggtga aggcgccgtt gcctggaagt tcatggtgat aggtctcgga
      301 aaggttcttc acagagctgt aggtgccatc cgaagtgtag gtgatatcgc atacgccatc
      361 ggtgccggtc atattctgaa cgaccgttgt cgtgtaatag ccatagtagt cagccataat
      421 caccgggatc acaacttcgg cggtgcctgt gctgacagcg gcgccattat agctgccgcc
      481 ctgagcatcg gtcgccgcca gagcttcctg gttggccagg acaacgaccg gaatatctga
      541 ggttacccgg accgagccga agaagcgagt gtagacattc gtatcgtcaa ggtcgctctg
      601 cacatcagtg gcggttgggc catcgtggcg ggttagtgag gtttgaggat cgattgtgaa
      661 gggtacagtg accggagcga tgctgccgct cgagacaaca ttggggcggc tcaggtcagg
      721 ggtataggtg atctcaacgt gagcagcggc actggttgaa ggattggcta caagcagggt
      781 gctgtagtag ccataatagc cgcgcatatg caccggagca gcgacatcta acgaaccacc
      841 atcggtgaag ccattatagg tcatcagctt ggagtcgttg acattgtgct gattgacgat
      901 agcgatgatg ggctggtctg aagtcaggat cgctgagccc aggaatctcc ctgcataagc
      961 tccgggagga gcgccaaggc cagattcagc ttgctggcta atgttgaggg aggcatactg
     1021 tgggacagca ttatctaaat cgtctaaagc agtcccggtt gcaacaccat tgacgacacc
     1081 agggatgtat tcgatgtcaa ccgaagccgc gccagtgcct acattcatga tgtagacttc
     1141 agtgtaatag ttgtaccagt tatacatgac agccggcagg atcactgaag tcgagccctg
     1201 gccttctgca ctgtaggtac cgaatggggg tgctggattg tagctggtgg cgccatcggg
     1261 tacaagctgc aggttagcaa tcgcggcgac cggctggtca gccgcgacca ctgccgagaa
     1321 gagcccactg ccgagcgcgg tatcatcggt gaactggaca accaggcgtg agctgccgga
     1381 aggtacatcg gtgaatacct gctggtgggt ttgctggacg ccattgccgt caaaatagct
     1441 gacggtgata tctgcgggcg catcgcccat attgacgacc tgatataccg acgtccagga
     1501 gaacccggca tattgagcgc caacggttcc tacgacgacg accaggatca ggagcgcaaa
     1561 agccgcacca attgtagttt ttcgtttcat atgaaatcct cctcgaattg tacttaaatt
     1621 gaatgctccg attttcctaa gtatatggat atcgatccaa ataaccaata gtccttatgg
     1681 gttaattgac gggagaacta tgttctgcgg gtaatccatc ggtattactc tgaccaaacc
     1741 aggcccagaa ggtttcaacc agagtaatta ataaacggta gactaatgaa agcacaatac
     1801 ctgctgagac gggcagccat gtacccagca tagcacccat cgtcaattcc tttaatccaa
     1861 agcctgccgg cagtatcata gtcagcattg cgaccagcgt cgatacagtt gatattccga
     1921 taaagctgac gacttgattg ggtaagggca aaaaggttgt cagggcgaag taaaacccga
     1981 tcccatccag gaaccaggga gccagataga gtaaccccca aatggtcaga tccctgcgtg
     2041 aaatgacgac atccagattg ggtttatggc ggtaattttg gattttattg atcaggtcga
     2101 ttaacagacc cggacggata aaaaatacgg ctaagaaaaa taccagcaaa ctgaccggaa
     2161 tgatccaggg gatccgttga gtgtaggcat accatggcag caagctcaga taacacacga
     2221 cgccaccgaa agccagcagc gcatgttcga gggctgttgc agtgagtgga atgctcggcg
     2281 ggatgtcttc tgcctgataa aggacaaccc ggctgcccac gaaccagatc gggagaggga
     2341 tatggcgggc caataacgac aggctgtaga tccgaaaatc tgtccgcagg ttgctccttc
     2401 ctgccaggcg cttcattgtc aggtgccagc ctgcaaacat gcagcctaac gatattgtgt
     2461 ggaaaatcac ataagccaat aaattgagcg gattgaagcc ccattcaaaa gagcggatga
     2521 cactccagtc gcgccagatc gcataaccca aaacgaggaa cactattcca gcgataatga
     2581 ggacccacca tttatttcgc gccgctgctt ttaaatccag agagctgaca gaaaatcctt
     2641 tcataaagtt ttatgtttca accgggaatg gcttggccag cacataaacc atttttgtga
     2701 ttgtggcttc ctggacccag ggaatccggg tggacagctg caccaattta taaattagcc
     2761 tggaggggat gtt
//
LOCUS       DATMXK010000296         2001 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_54204, whole genome shotgun sequence.
ACCESSION   DATMXK010000296 DATMXK010000000
VERSION     DATMXK010000296.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2001)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2001)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2001
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54204"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1538)
                     /gene="asnB"
                     /locus_tag="VN363_05460"
     CDS             complement(<1..1538)
                     /gene="asnB"
                     /locus_tag="VN363_05460"
                     /EC_number="6.3.5.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR01536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004066 - asparagine synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006529 - asparagine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="asparagine synthase (glutamine-hydrolyzing)"
                     /protein_id="HWQ83991.1"
                     /translation="MCGITGIVKHNPGSLVDEALLRQMLGMLRHRGPDQFGIYVDEQA
                     GLGSARLSIIDLSSGQQPISNEDETLWIVFNGEIFNYIELRPELEKRGHHFATHSDTE
                     VILHLYEDYGPQCLSYLNGQFAFAIWDSRQQSLFLARDRVGIRPIFYTETGSALVFGS
                     EIKAILAYPGVQAELDPAALDDTFSYWSPQSPRSAFKGIYEVPPGSYMLVQNGQTRIE
                     RYWQLEYPDTSRDEYAASLKRPEEDYLAEFQKLLIDATQIRLRADVPVGAYLSGGLDS
                     STITAIIRQFTGNRLDTFSISFSDPEFDESDFQRRMAQHLGTDHQVVYATHADIGRIF
                     PDVIWHTETPVTRTSPAPMHLLSRLVRQHNFKVVLTGEGADEFLGGYDIFKEAKIRRF
                     WAREPESEGRQLLLQKLHPHIKDLTREGGAYLAAFFKDGLTGVDRPTYSHDIRWKTTR
                     RTKRFFSPGLVEQIRQRTPFNLELPDGFSRWDPLAKAQYIEITTFMSPYLLSSQGDRP
                     GMANS"
     gene            complement(1552..>2001)
                     /locus_tag="VN363_05465"
     CDS             complement(1552..>2001)
                     /locus_tag="VN363_05465"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560398.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-dependent synthetase"
                     /protein_id="HWQ83992.1"
                     /translation="VVRGRHVMRGYWENPAATAERYRPGPIPGERLCYTGDLFRMDEE
                     GFLYFVSRKDDIIKSRGEKVAPKEVETVLYSIPGVVEAAVVGVPDTIIGEAVKAFLVL
                     DGAELSEAQVLAYCRAHLEDFMVPKMVEFRDALPKTSSGKIKKTDLG"
BASE COUNT          464 a          551 c          546 g          440 t
ORIGIN      
        1 gagttcgcca tgccggggcg atcgccctgg gaggaaagca ggtaaggcga catgaatgtg
       61 gtgatttcga tgtactgtgc cttggccaac ggatcccagc gagaaaatcc atcgggcagc
      121 tccagattaa agggtgtcct ttgtcgaatc tgttcaacca gaccgggtga gaagaaccgc
      181 tttgtgcgcc gggtagtctt ccaccggata tcatgcgaat aggtcgggcg atccacaccg
      241 gtcaggccat ctttgaagaa tgctgccagg tatgcgccgc cctcccgggt caggtctttg
      301 atgtgagggt gcagcttctg aagtaagagc tggcggcctt ctgattccgg ttccctggcc
      361 cagaaacgcc tgatcttggc ttccttgaaa atatcgtatc cacccagaaa ttcatccgca
      421 ccttcaccgg tcaggacaac tttaaaattg tgctggcgga caagcctgga gagcaggtgc
      481 atgggagcgg gcgaggtgcg ggtgaccggg gtctcggtat gccagatgac atccgggaaa
      541 atccgtccaa tatcagcatg ggtggcatag acaacctggt ggtcagtccc caggtgctgc
      601 gccatccggc gctggaagtc gctttcgtca aattcaggat cgctgaaact gatcgaaaaa
      661 gtatccaggc gattgccggt gaactggcga ataatcgcgg taatggtcga agaatccaga
      721 ccgccgctca agtatgcgcc aaccggcaca tctgcgcgca ggcggatctg ggtggcgtca
      781 atcaatagct tctgaaattc agccaggtaa tcctcttcag ggcgtttgag gctggctgca
      841 tactcatccc tggaagtgtc gggatactcc agctgccaat agcgctcaat ccgggtctgg
      901 ccattctgaa cgagcatata cgaacccggc ggaacttcat aaatgccttt gaacgcactg
      961 cgcggggact gcgggctcca atagctgaag gtatcatcca gcgcagcggg atccaattcc
     1021 gcctgaactc ccggataagc caggatcgct ttgatttcag agccaaagac aagcgcgctg
     1081 cccgtttccg tataaaagat tggccggatg cctacccgat cgcgggccag gaacaggctt
     1141 tgctggcggc tatcccagat ggcgaacgcg aactgcccat tcagatagct caggcattgg
     1201 gggccgtaat cttcatacag gtggagaatt acttctgtat cactgtgggt ggcaaaatga
     1261 tggccgcgct tttcgagctc ggggcgcagc tcgatataat taaagatctc gccattgaag
     1321 acaatccata aggtctcatc ttcgttggag atgggctgct gaccactgct caagtcgata
     1381 atgcttaatc gagcactgcc gagaccagcc tgttcatcta cataaatccc aaattggtcc
     1441 gggccgcggt gcctcagcat cccaagcatc tggcgtaaga gagcttcgtc gaccaggctg
     1501 ccaggattat gttttacgat accggtaatt ccacacatct cacaccgcct gttatcccag
     1561 atccgtcttc ttgatcttac cggaggaagt tttcggtaat gcgtcgcgaa attcaaccat
     1621 tttcggaacc atgaaatctt ccaggtgggc acggcagtaa gccaggacct gggcttccga
     1681 aagctcagcc ccatccagca ccaggaaggc tttgacagcc tcgcctataa tggtgtcagg
     1741 tactccaaca actgccgcct ccacgacccc tgggatggaa tacaataccg tctcgacttc
     1801 cttaggggcg actttttcgc cgcgactttt gatgatatcg tccttacggc tgacgaaata
     1861 aagaaaacct tcttcatcca tccggaaaag atcgccggta taacacagcc gttcgcccgg
     1921 aatgggtccg gggcgatagc gctcggcagt tgcggcagga ttttcccagt acccacgcat
     1981 cacatgccgg ccgcgcacca c
//
LOCUS       DATMXK010000297         7882 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51334, whole genome shotgun sequence.
ACCESSION   DATMXK010000297 DATMXK010000000
VERSION     DATMXK010000297.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7882)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7882)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7882
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51334"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(107..2602)
                     /locus_tag="VN363_05470"
     CDS             complement(107..2602)
                     /locus_tag="VN363_05470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83993.1"
                     /translation="MSQALDLHLFSLVRSAGQDQTEPSGLHVASPPRRAARSRKADQL
                     VLHLSLAGNAPLAPAHQDQLLARLAQVYYQSSGSVTAALRSLAEALNKYLLERNLKNA
                     GSGRQAIGLLSLVVFKENQLYLAQSGPVHAYHLTAGNAQHIFDPQISGRGLGLSRNTP
                     IRFFHALLEPNDTLLVATEADPSWSQVTLASLHGQGPESQRRKLLGRAGLDINAYMLQ
                     ARPGKGLVTRQPAQSTRPAVTAGETPLVEMPVGVQLAPEQPEALDQSQTAADEGQPIE
                     FVSQVPPAQISEPGSAPLEPVQTTETVEEIEPEEEPDEIEVGEDEAPASDELDQVPWK
                     PLPQELPKIEQTAPSAAVDKSAAGDREASIINASNSNAVRNRLEQAGRAARPARSARR
                     KGLDTSPLASALASAGRPIGGAMGAAGSKLKTVLERILPDESIFTIPTSFMAIIAIAV
                     PLIIVVIATGVYTQLGRTVQYQTLFTQAAEQVRLADSQTDPLARRTGFHQSLVLLEQV
                     EAYGITAETQEMRQHIQLAMDDLDNVRRLNYQPAIIGGLAEDISIARMLVVDNDLYLL
                     DRNSGQVLRGLVTNNGYEIDRSFQCGPNASLVYQIGPLVDITYSPVNSVNAAVMGMDA
                     RGGIIKCYTSGSPDVEVLTLPSRVVDGDLTSLTYEQGNTYVLDPLNRAVWIYWNSKFD
                     EEPQFFFGEEVPNLDDVVDMAVEKEQLYLLRATGNMAVCTYSGLVVSPTRCMDPAPYV
                     DYRVDPAGVPLQWTDPFGAVLTTSSPDPSLYLFSPQTNAVFQFSLRTLAIQRQFMPAE
                     ALGNGAGTALAVNPVDRILIVAVHHRVYYAFIP"
     gene            2750..3799
                     /locus_tag="VN363_05475"
     CDS             2750..3799
                     /locus_tag="VN363_05475"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ83994.1"
                     /translation="MQLALLRSTSEFQSLADEWNSLLEESASHVPFLRHEYLFAWWQT
                     LGGGEWKNTTGSEPELCLVTGRAPDGSLLGIAPLFFTRNREGEPALMLLGSIEISDYL
                     DLIVAPQDLDTFIPALLDFLVTSPDLPAWSVLDFYNLIETSPTLPSIQSAARTRGWID
                     QIEKLQHCPAIPLASDWDSYLAGIDKKQRHEIRRKMRRIESPEQAARWYIVSDPARLA
                     GEIESFFDLMAFDPEKKAFLTPEMRTQMRLAYEAGFKAGWLQLAFIEIDGEKAAAYLN
                     FDYDNQIWVYNSGINYDLGALSPGWVLLGYLIQWAIEHGRASFDFMRGDEDYKYRFGG
                     IDRRIQRIILRRPTP"
     gene            complement(3802..4920)
                     /gene="amrS"
                     /locus_tag="VN363_05480"
     CDS             complement(3802..4920)
                     /gene="amrS"
                     /locus_tag="VN363_05480"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561332.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system radical SAM enzyme"
                     /protein_id="HWQ83995.1"
                     /translation="MIMLGEFLDNLTTEGELVEKLDDGGVRCVACGHRCLIRPGRRGI
                     CQVRFNLDGELRVPWGYVAALQADPIEKKPFFHVYPGVDALTFGMLGCDFHCGYCQNH
                     LTSQAMRDPGSDIAASLVRKVTPTELTRLAQRAGAQVVVSSYNEPLITSEWAVDVFQE
                     ALKAGLRCAFVSNGNATPEALRYLRPYLSAYKIDLKTMQDRQYRSLGGVLQHVLDSIR
                     LAYELGLWVEVVTLVVPGFNDSTEELLDAARYIRSVSADIPWHVTAFHPDYKMLDPSP
                     TSVKTILRAAEIGQEAGLNFVYAGNIPGRVGEYENTTCPACHALLIERTGYTIHGYHL
                     TASGNCPQCGTAVPGVWPENAQQVRLGGPGIPRWVRNR"
     gene            5045..5665
                     /locus_tag="VN363_05485"
     CDS             5045..5665
                     /locus_tag="VN363_05485"
                     /EC_number="2.7.7.77"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0061603 - molybdenum cofactor
                     guanylyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdenum cofactor guanylyltransferase"
                     /protein_id="HWQ83996.1"
                     /translation="MRSLVIQAGGQSRRMGQDKALLPFLGQPLIQRGIARLGHLADEI
                     LVTTNHPENFTFLGLPLFPDIIPDRGALSGLYTALHASRHDTVLVVACDMPFISPALL
                     GAQADLLEQGYDAVIPLGEGGTEPFHSVYRRSTCLELALAAIQAEKWRVDAWFPQANI
                     HFLNETEIQRLDPSGMSFWNVNTPAEFQRAEAIARQLDQGNNHEPE"
     gene            5652..6914
                     /locus_tag="VN363_05490"
     CDS             5652..6914
                     /locus_tag="VN363_05490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559695.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cysteine desulfurase-like protein"
                     /protein_id="HWQ83997.1"
                     /translation="MSLNNPTIRQQFPALEKPAIYLDNPGGTQIARRSLERIQGYLRD
                     TNANHEGAFATSRASDALIEAARSAAASFLCANRPEEIVFGPNMTSLTFNLSRSIART
                     WSPGDTIAVTRLDHDANITPWVMAAQDRGCRVRWVDFHPEDGTLDLEDLQAALAEKPR
                     LLAVGYASNALGTINPLEKIIPWAHAAGTQVYVDAVQYAPHGPIDVQALDCDFLVCSA
                     YKYFGPHVGVLFGKYDLLDRLTAYRVRPAPQTPPGKFETGTGNFEGYAGVLGAIEYFE
                     WLGEAFGGEQAESYSGLYQGKALHLKQAMSAIRAYEFELSSALLDVLEETPGVKVYGI
                     TDRRRLNERVPTISFTLKGLHPRQVAEALDKENIYVWDGNYYALAVTERLGLEKSGGM
                     VRVGPVHYNSIQEIEKLGEALRKIIPPR"
     gene            complement(6985..7461)
                     /locus_tag="VN363_05495"
     CDS             complement(6985..7461)
                     /locus_tag="VN363_05495"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ83998.1"
                     /translation="MMSTIESELFTKALLEILDETFDNVQGYFLDRGTSLFETLATIS
                     AEEASIPVGGKCATLAAQVKHVAFYLNVVDKNVREHNYARVDWGEIWRTVNAVTPEEW
                     SAIQAELREAYNRIISLVTEMPAWTSADEIGGAIGLVAHTAYHLGEIRQALCTLKP"
     gene            complement(7462..7803)
                     /locus_tag="VN363_05500"
     CDS             complement(7462..7803)
                     /locus_tag="VN363_05500"
                     /inference="COORDINATES: protein motif:HMM:NF016246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF488 family protein"
                     /protein_id="HWQ83999.1"
                     /translation="MIRLKRAYEKPGSEDGQRILVERLWPRGLTKEQAQIDRWEKDIA
                     PSPELRKWYAHDVTRWDEFQRRYRLELEHNPDIGRFQQALGEVELVTFVFAARDAEHS
                     SARILRDFLEG"
BASE COUNT         1731 a         2291 c         2108 g         1752 t
ORIGIN      
        1 gccctggcca accaataccg ccgcacccta ataaaaactg cctggaattg gttctccagg
       61 cagtttttat taatttcagc gattcagatc tcttatagat ccagccttaa ggtataaaag
      121 cataataaac ccggtggtgg accgccacaa tcaaaatgcg atctacaggg ttaaccgcca
      181 gagcggtgcc tgccccattc cctaatgcct cagccggcat gaattggcgt tggatcgcca
      241 gggtgcgtaa gctaaactgg aagaccgcat tggtctgagg cgaaaacaaa tataaagatg
      301 gatcgggcga agacgttgtc aacacggcgc caaatgggtc ggtccactgc aaggggaccc
      361 ctgccgggtc cactcgataa tcgacatacg gcgctggatc catacagcgc gtgggggata
      421 cgaccagccc gctgtaggta cagacagcca tattgccagt agcacgcagc agatataact
      481 gttccttttc tacagccata tccaccacat cgtccaggtt cgggacctct tcgccaaaga
      541 aaaactgtgg ttcttcatca aacttactgt tccaataaat ccacacggcc cgattgagcg
      601 ggtctaatac ataggtgttg ccctgctcat aagtcagcga agtcaggtcg ccatcgacca
      661 cccggctggg gagagtcaat acctccacgt ctggcgagcc gctggtatag catttaatga
      721 ttcccccgcg ggcatccata cccatcacag ccgcattgac gctgttaacc ggtgaataag
      781 tgatatccac cagcgggccg atctgataaa cgagtgaagc atttggacca cactggaaag
      841 agcgatcaat ttcatagcca ttattcgtca ccagaccgcg caacacctgc cctgaattgc
      901 ggtccagcag atacaggtcg ttatcaacaa ccagcattct ggcaatggat atatcttcag
      961 ccagcccacc gataatggcg ggctgataat tcaaccggcg gacattatcc agatcatcca
     1021 tagccagctg gatatgctga cgcatttcct gggtctcagc agtgatccca tacgcttcaa
     1081 cttgttccag caagaccaat gattggtgga atcctgtcct gcgagccagt ggatcggtct
     1141 gtgaatcggc caaccggacc tgttcggcgg cttgagtaaa cagcgtctgg tactggaccg
     1201 tacgccccag ctgggtgtaa accccggtgg caatcacgac aataatcaag ggcaccgcaa
     1261 tcgcgataat tgccatgaac gaggtgggga tcgtaaaaat cgattcgtcc ggtaagatcc
     1321 gctccaggac tgttttcagc ttgctgccag ccgcgcccat cgccccacca atcgggcggc
     1381 cggctgaggc cagcgcagaa gccagcggag acgtatccaa ccctttccgt cgcgcactgc
     1441 gcgctggacg agcagctcgt ccagcctgct ccaaccggtt ccggacagca ttactattgc
     1501 tagcattaat tatgctagcc tcacgatcgc cagcagcact tttgtccact gcggcgctcg
     1561 gggcagtctg ctcgattttc ggaagttcct ggggtagagg tttccagggg acctggtcca
     1621 gctcatcact ggccggcgct tcatcttcac ccacctcaat ttcgtctggt tcctcttcgg
     1681 gttctatttc ttcaaccgtc tccgtggttt ggacaggctc aagtggggca gaaccaggtt
     1741 ctgagatctg cgccggtgga acctgagaga caaactcaat tggttggcct tcatcagctg
     1801 ctgtttgact ctgatccagc gcttctggct gctcaggggc gagctgaaca ccaacgggca
     1861 tttcgacgag cggggtttcc cctgccgtca cagccgggcg ggtagactga gcaggctggc
     1921 gcgttaccag gcctttaccg gggcgggcct gaagcatata ggcattgata tccagcccgg
     1981 cacggccgag cagcttccgc cgctggctct ctgggccttg cccgtgcagg cttgccaggg
     2041 ttacctggct ccagctcggg tcagcctcag tcgcaaccag gagggtatca ttgggctcca
     2101 gcaaagcatg aaaaaagcgg atcggcgtgt tacggcttaa acccagaccc cgtcctgaga
     2161 tttgcggatc gaagatatgc tgggcattcc cggctgtcag atggtaggca tgcaccggcc
     2221 cggactgcgc caggtaaagc tggttttcct taaaaacgac caggctcagc agaccgatcg
     2281 cctgacgccc gctgccagca ttttttagat ttctttccag gaggtatttg ttcaacgcct
     2341 cggccaggct ccgcaaagca gccgtcaccg agccagagct ctgataatat acctgggcaa
     2401 ggcgcgccag cagctgatcc tggtgagccg gggccaaagg tgcatttccg gccaggctca
     2461 agtgcaggac aagctggtcg gctttacggc tgcgtgccgc ccgccgagga ggactggcga
     2521 catgcagccc gctcggttca gtctggtctt gtccggcaga gcgtaccagg gagaataggt
     2581 gaaggtctaa ggcttgggac atggtcattc acatcttaca ctttcaaggg tgatgaaatc
     2641 caattggaca aattatactg gtaaaatgca ggatagatga gctacgcgct ggatcactca
     2701 atccatttgc ctggctgatc ccatttattg aggatgggga agataatcaa tgcagctggc
     2761 cctcctgcgt tcgacctctg aattccaatc gctggcagat gaatggaact ccttgctgga
     2821 agagagtgca agtcacgtgc catttctccg ccacgaatac ctgtttgcct ggtggcagac
     2881 acttggcggc ggtgaatgga agaataccac aggttccgag cctgagcttt gtctggtcac
     2941 cggtagagct ccggatggca gcctgttggg tattgcgccc ctgttcttca cgcgaaaccg
     3001 cgagggcgag ccagccctta tgctgctcgg aagcatcgaa atatcggatt acctggatct
     3061 gatcgtagcg ccgcaggatc tggatacctt tatccctgcg ctgctggatt ttctggttac
     3121 cagccccgat ttacccgcct ggtctgtgct ggatttctac aatctgatcg aaacctcacc
     3181 taccttgcca tctatacaat cagccgccag aacccgcggt tggatcgatc aaattgaaaa
     3241 attacagcac tgcccggcga tccccctggc gagtgattgg gatagttacc tagccgggat
     3301 tgataagaaa cagcgtcacg agatccgccg taagatgcgc cggatcgaaa gcccggaaca
     3361 ggctgcccgc tggtatatcg tttctgatcc cgccaggctg gcaggtgaaa ttgagtcgtt
     3421 ctttgacttg atggctttcg atcccgagaa aaaagccttc ctgactcctg aaatgcgcac
     3481 ccagatgcgc ctggcttacg aggctggctt taaagctggc tggctgcagc tggcttttat
     3541 tgagatcgac ggcgaaaaag ctgccgccta tctgaatttt gattacgaca accagatctg
     3601 ggtttacaat tcggggatta attacgacct gggtgcgctt tcaccgggat gggtactgct
     3661 gggttatttg atccagtggg caatcgaaca cggccgcgcc agctttgatt tcatgcgcgg
     3721 cgacgaagat tacaaatatc gctttggcgg cattgaccgg cgcatccagc ggatcatttt
     3781 gcgccgtcca accccctgag attaccggtt tctcacccag cgtgggatac ctggcccgcc
     3841 cagccgcacc tgttgggcgt tttcaggcca gacccctggc acagccgtcc cacactgcgg
     3901 gcagtttcca gacgcggtca ggtgatagcc gtgaatggta tagccggtgc gctcgatgag
     3961 cagggcatgg caggccgggc aggttgtatt ttcatattcg cctactcgcc cagggatatt
     4021 cccggcataa acaaaattca gtccggcttc ctgcccgatc tcagctgcgc gcaggatcgt
     4081 tttcaccgag gtcggcgagg gatccaacat tttataatcc ggatggaagg ccgtcacgtg
     4141 ccaggggata tccgctgaga ctgagcgaat atagcgggct gcatccagca attcctcggt
     4201 gctgtcatta aatccaggca cgaccagggt gactacctcc acccacaggc ccagctcata
     4261 ggccaggcga atgctgtcca gcacatgctg gagcacccca cccagactgc ggtactggcg
     4321 atcctgcatt gtcttcagat cgattttgta agccgaaaga tagggccgca gatagcgcag
     4381 cgcttcgggg gtcgcattcc cattggatac aaatgcgcag cgcagtccgg cctttaaggc
     4441 ttcctggaac acatcgaccg cccattccga ggtgatcagc ggctcattat aggaagatac
     4501 aactacctgc gccccggcgc gctgcgccag tctggtcagc tcggtcgggg taactttccg
     4561 caccagggag gcggcgatat ccgatcccgg gtcgcgcatc gcctgggagg tgagatggtt
     4621 ctggcagtaa ccgcagtgaa aatcgcagcc cagcatacca aatgtcagcg catcgacgcc
     4681 tggataaacg tggaaaaacg gctttttttc gatcggatca gcctgcagag cagcgacata
     4741 tccccatggg actcgcagct cgccatccag gttaaagcga acctggcata ttcctcgccg
     4801 tcctgggcga atcaggcagc gatgtccgca ggcgacgcag cgtaccccgc cgtcatctaa
     4861 tttctctacc agctccccct cggtcgttaa attatctaag aattctccca gcataatcat
     4921 gtactttact ccatctatat ccttttacca ataagcgcag ggagcagtga aacaggcacg
     4981 ttcaggggaa tctccggtca ggcaaactgc ccgcgtagga gatgcatcta ttataatcaa
     5041 aatcatgcgc agtttagtta ttcaggcagg tgggcagtcc cgtcgtatgg ggcaagataa
     5101 agctctgctg cccttccttg gacagccatt aatccagcgt gggatcgccc gcctcggtca
     5161 cctggcagac gaaatcctgg tcaccaccaa ccatcctgaa aattttacct ttttggggct
     5221 gccgctcttt ccggatataa tcccggaccg tggggcattg agtggattgt acacggcttt
     5281 gcatgcatcc cgccacgaca cagtcctggt cgtggcctgc gatatgcctt tcatttcccc
     5341 cgcgcttctg ggtgcacagg ccgacctgct cgaacagggt tacgatgctg ttatccccct
     5401 tggcgaaggt gggacagagc cgttccattc tgtataccgc cgctccacct gcctggagct
     5461 ggccctggct gccatacagg ctgagaaatg gcgggtcgat gcctggttcc cccaggctaa
     5521 catccacttt cttaacgaaa ccgagatcca gcgcctggat ccctctggaa tgagcttttg
     5581 gaatgtcaat acccccgcag aatttcagcg cgccgaagca atcgcccgcc agctcgacca
     5641 aggaaacaac catgagcctg aataacccca ccatccgtca gcagttcccc gccctggaaa
     5701 agcccgccat ctaccttgat aatcctggtg gcacgcagat cgcccgccgc tccctggagc
     5761 gtatacaggg ttatctgcgc gacaccaacg ccaaccacga gggcgccttc gcgaccagcc
     5821 gcgcctccga cgccttgatc gaagccgccc gctccgcggc tgccagcttc ctgtgcgcta
     5881 accgccccga ggagattgtc ttcgggccga atatgacctc cctgaccttc aatctcagcc
     5941 gctcgattgc ccgcacctgg tcgcccggcg acacgattgc ggtcacccgc ctggaccatg
     6001 atgctaatat caccccctgg gtcatggcag ctcaggatcg cggctgccgt gtgcgctggg
     6061 tggatttcca tcctgaagat ggcacccttg atcttgagga tctgcaagct gccctggcgg
     6121 agaaaccacg cctcctggca gtaggctatg cctccaatgc cctgggcaca atcaacccgc
     6181 tcgagaaaat tatcccctgg gcccatgctg ctggcacgca ggtgtatgtg gatgccgtcc
     6241 agtatgcgcc gcatggtccg atcgatgtac aggcgctgga ctgcgatttc ctggtctgtt
     6301 cagcctacaa atatttcggc ccgcatgtgg gagtcctgtt tggcaaatat gatttgctgg
     6361 acaggctgac tgcctatcgg gtgcgccctg cgccgcaaac accgcccggc aagttcgaga
     6421 ccggcactgg aaattttgaa ggctatgccg gcgtgctggg agctatcgag tatttcgaat
     6481 ggcttgggga ggcattcggc ggagagcagg ctgaatccta ttccgggctt tatcagggaa
     6541 aagccctcca cctcaagcaa gcgatgagcg ccatccgcgc ctatgaattt gagctttcaa
     6601 gtgcattgct ggatgttctg gaagaaactc ccggtgtcaa agtctacggg attaccgacc
     6661 ggcggcgatt aaatgagcgt gtaccgacca tcagcttcac tttgaaaggg ttgcaccccc
     6721 gccaggtggc ggaagcactg gataaagaaa acatttatgt ctgggacggg aattattatg
     6781 cgctggcagt caccgagcgc ctggggctgg aaaagagtgg cggtatggtc cgtgtcggac
     6841 cggtgcatta caattcaatc caggaaatcg agaagttggg ggaggcactc cggaagatca
     6901 tcccgccccg gtgagtgatg gccttatcct ctggtttttt aaaccttaat ttcgcgatac
     6961 tcagcggaaa atgtcggatt attgttatgg tttcagggta cacagcgcct ggcggatttc
     7021 acccaggtga tacgcggtgt gcgcaacaag accgatcgct ccgccaatct cgtctgcact
     7081 cgtccaggct ggcatttcgg tgaccaggct gataatcctg ttataagcct cccgcagctc
     7141 ggcctggata gcgctccatt cctcaggtgt gactgcgttg actgtgcgcc agatctcacc
     7201 ccaatccacg cgagcataat tatgctcacg aacattttta tcgacgacgt tcaggtagaa
     7261 tgcgacatgc ttgacctgcg cggcgagggt agcgcatttc cctccgactg gtatggaggc
     7321 ctcctcggca ctgatcgtcg ccagagtctc aaaaagcgat gtcccccggt ccagaaagta
     7381 accctgcaca ttatcaaagg tctcatccaa tatctccagc agagctttgg taaagagttc
     7441 agactcgatc gttgacatca ttcatccctc tagaaaatct ctgagtatcc ttgcactgct
     7501 atgttcggca tcccgcgccg caaacacaaa agtgaccagc tctacttcac ccagggcttg
     7561 ttgaaaccga ccgatatctg gattgtgctc cagctccagg cgataccggc gctgaaattc
     7621 gtcccagcgc gtcacatcgt gggcatacca tttacgcagc tccgggctgg gagcaatatc
     7681 tttttcccag cggtcgatct gagcctgctc tttcgtcaga ccccgcggcc acagccgttc
     7741 cacgaggata cgctgcccgt cctcagagcc aggcttttcg taggcgcgtt tcaagcggat
     7801 catccatacg ctctcctttg ccatcccgga aaccagagtc cgtccttaaa tggcgagcat
     7861 cgaaatttaa ctcggattgg at
//
LOCUS       DATMXK010000298         2022 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_163163, whole genome shotgun sequence.
ACCESSION   DATMXK010000298 DATMXK010000000
VERSION     DATMXK010000298.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2022)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2022)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2022
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_163163"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..231)
                     /locus_tag="VN363_05505"
     CDS             complement(<1..231)
                     /locus_tag="VN363_05505"
                     /inference="COORDINATES: protein motif:HMM:NF014273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ParB N-terminal domain-containing protein"
                     /protein_id="HWQ84000.1"
                     /translation="MSRRTGLGRGLDALIPGGENNSGAVGVEQIEVERIVPNPRQPRT
                     DFDPAALAELAESIREHGILQPLIVSSGDQPGT"
     gene            complement(244..1035)
                     /locus_tag="VN363_05510"
     CDS             complement(244..1035)
                     /locus_tag="VN363_05510"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWQ84001.1"
                     /translation="MARTYTFVNQKGGVGKTTTAINLGAYLAYYGQRVLLVDLDPQAN
                     ATSCLKIDKNSVRGGTYDVLIGNSMPGAYILHNPRLKMSLLPASTALAGAEVELVSEL
                     ARETRLKKAVSQLQDRYDYILIDCPPSLGLLTLNGLVAAQDGVIIPVQCEYLALEGLS
                     QLIKTLNRVRSALFPELRVRGVVMTMYDARTKLANDVVGEVQTYFPDQVFKAVIPRSV
                     RLAEAPSYGQPISVYAPASTGAEAYAALAREVLAGDGLPIPIPQV"
     gene            complement(1044..1892)
                     /locus_tag="VN363_05515"
     CDS             complement(1044..1892)
                     /locus_tag="VN363_05515"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWQ84002.1"
                     /translation="MLNLKALEPVDYLIIGHLTVDLTSEGPRLGGTALYSGLTARALG
                     LRVGIVTSWADEIPLGPLGAIPVASYPAEHSTTFQNDYTPQGRQQTILHVASSLDLYH
                     VPEVWRSAPIVHLAPVAQEVEPAVVRGFPSALVGVTPQGWMRSWDQDGRVQVAEWPEA
                     DFVLSRCGAAILSIEDVGYDEERIEEIAASSRVLAVTEASQGARLYWNGDVRRFRPPR
                     VQEVDPTGAGDIFATAFFSRLYITRDPWEATRFATHLAAMSVTRPGLQGIPTLQEIEE
                     GLVQVF"
BASE COUNT          466 a          604 c          560 g          392 t
ORIGIN      
        1 cgtccctggc tgatcgccag agctgacgat caagggctgc aggatgccat gctcccggat
       61 cgactcggcc agttcagcca gggctgccgg gtcaaaatct gtgcgtggtt ggcgcgggtt
      121 cggcactatc cgttccactt caatctgctc gacgcctacc gccccagaat tattctcccc
      181 tcctgggatc aacgcatcga gaccacgacc aagtccggtt ctacgcgaca tacaacacct
      241 atctcagacc tgtggaatcg gaatgggcaa accatccccg gctaaaactt cccgggccag
      301 ggctgcgtaa gcctcggcac ccgtggaagc cggggcataa acactgattg gctgcccata
      361 ggagggggct tctgccaggc gcacgctgcg ggggatgacc gctttgaaca cctgatctgg
      421 gaagtaggtc tggacctcgc ccaccacgtc attcgccaat ttcgtgcgcg catcgtacat
      481 ggtcatcacc accccgcgca cccgcagctc cgggaaaagg gcactgcgca cccggttcaa
      541 ggttttgatc agctggctga gcccttccag cgccaggtac tcacactgca ccgggataat
      601 gaccccgtcc tgggcagcca ccagaccatt taatgtcagc aatcccaggg acggcgggca
      661 gtcgatcaaa atataatcat agcgatcctg gagctgcgag acagcttttt taagccgggt
      721 ttcccgcgcc agctcggaaa ccagctccac ctccgcccca gccagcgccg tagaagccgg
      781 aaggagcgac attttcaaac gcgggttgtg caggatgtaa gctccgggca tcgaattgcc
      841 aatcaggacg tcgtaagtac cgccgcgcac cgaattctta tcgatcttaa ggcaggaagt
      901 ggcattggct tgcgggtcca aatctaccag tagcacgcgc tgcccgtaat aagccagata
      961 agcgccgagg ttgatggctg tggttgtttt gcctacgccc cctttttgat tgacgaatgt
     1021 gtatgtacgc gccataggct ctcttaaaaa acttgcacaa gcccttcttc gatttcctga
     1081 agggtgggta tcccctgtaa accagggcgg gtgacggaca tggctgccag atgggtggca
     1141 aaacgagtag cttcccacgg atcacgggtg atgtagagac gggagaaaaa ggcagtcgca
     1201 aaaatatcgc ccgcaccagt ggggtctact tcctggaccc ggggtgggcg aaagcggcgc
     1261 acatctccat tccaatacag gcgggcgccc tgggaggctt cggtcacagc caacacccgg
     1321 cttgaggccg ctatttcttc aatccgttct tcatcataac caacatcttc aatactgagg
     1381 atggctgccc cgcagcggct gagcacaaaa tccgcctcgg gccattctgc aacctgcacg
     1441 cgcccatcct ggtcccagga acgcatccag ccctgggggg ttaccccaac caacgccgaa
     1501 ggaaaacccc gcaccaccgc tggctcaact tcctgtgcca cgggagccag gtgcacgatt
     1561 ggcgcgctgc gccagacttc gggaacgtgg tagagatcca gggaagaggc aacatgcagg
     1621 atcgtctgct ggcgaccctg gggagtataa tcattctgga aggtagtgct gtgctcggca
     1681 ggataactgg caaccgggat ggcacccaga ggacccagag ggatttcatc tgcccagctg
     1741 gtgacgatcc ccacccgcaa tcctaaagcg cgggcagtca acccggaata aagggcggtg
     1801 ccacccaggc gcggtccttc cgaggtcaga tcgactgtca gatgaccgat aattaaataa
     1861 tcaaccggct caagagcttt aaggttaagc atgggtgtat tataccgtga gaacagccca
     1921 gggacgcccg ccggaagtct gtgaacagat tattctgaac gcagaaattt gttttacaaa
     1981 tctaaatcca catggtataa tttcaacccg cgccgccaat gg
//
LOCUS       DATMXK010000299         2105 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_66509, whole genome shotgun sequence.
ACCESSION   DATMXK010000299 DATMXK010000000
VERSION     DATMXK010000299.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2105)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2105)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2105
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_66509"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..265)
                     /locus_tag="VN363_05520"
     CDS             complement(<1..265)
                     /locus_tag="VN363_05520"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_010925670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWQ84003.1"
                     /translation="MRRKPAKPIFKTYTQAQPSLIPPSWDELIPAEHVVRVVNQALDQ
                     IDLEPLMSQYKGGGTSSYHPGMMLKVLVYAYTQRIYSSRQIAKA"
     gene            complement(355..>2105)
                     /locus_tag="VN363_05525"
     CDS             complement(355..>2105)
                     /locus_tag="VN363_05525"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024938.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84004.1"
                     /translation="LATDALITNGGQLTELSDETMQAYNQILPPTWSHNNPVDIIGDA
                     SPDRYAAALEIAANDTNSDGILVILTPQAMTDPTATAEKLVPYAHNLGKPVLASWMGG
                     ADVEKGDMILNQANIPTFAYPDSAARMFDYMAHYSTFLRLLYETPMLPDGDVEPRREH
                     AAEIIQHVRQTGRTILTEYESKQLLSAYGIPTVETRIAKSADEAVKIADELGYPIVVK
                     LHSETITHKTDVGGVKLNLRDANAVRAAFENIFKSVSEKAGAEHFLGVTVQPMLPLGG
                     YELILGSSIDPQFGPVLLFGMGGTLVEVFKDRALALPPLNTTLARRMMERTTIYTALR
                     GVRGRKSVDLPALELMLVRFSQLIAEQRWIKELDINPLLASEDRLLALDARVVIHDPE
                     VTEDRLPRLAIRPYPSQYISQWTAKDGSPVTIRPIQAEDEPALVKFHETLSDRTVYMR
                     YLKPMLLSDRVAHERLARIAHSDYNREITLIAEQFTVPDMETAVIMGVTRLSKIHGSN
                     EARFTTLVNDQYQGKGIGTELIRRMIDVARAERLDRIEALISPDNLAMQNLCQKLGFE
                     LKPTADNTMVVAEMKF"
BASE COUNT          405 a          578 c          622 g          500 t
ORIGIN      
        1 gcgctttggc gatctggcgc gatgagtaga tccgctgggt gtaggcgtac accaacactt
       61 tcagcatcat ccccggatga tagctgctgg ttcctccccc tttgtactgg ctcatcagcg
      121 gctccaggtc gatctggtcg agcgcctggt tgaccacccg caccacatgt tcggctggaa
      181 tcaattcatc ccagctaggc gggatcaggc tcggctgtgc ctgggtatag gttttgaaga
      241 tcggtttggc tggttttctt ctcatacccc ctattgaatc acagtttcct tgtttcgcga
      301 agggggctgc ccagttcttt ttggacagcc cctttggttt gtggatcacg ctgtttagaa
      361 tttcatctcg gcaaccacca tggtgttatc tgctgtcggc tttagctcga aaccgagctt
      421 ctggcagaga ttttgcatgg cgaggttgtc tggtgaaata agcgcctcaa tccgatcgag
      481 cctctcagcc ctggcgacat cgatcatgcg ccggatcagc tcggtgccaa tccccttacc
      541 ctgatactgg tcattgacca gcgtggtaaa gcgcgcctcg ttgctgccat ggattttgct
      601 caggcgggtt acgcccatga ttaccgcagt ctccatatcc ggaacggtga actgctcagc
      661 gatcagggtt atctcccggt tatagtcgct gtgggcaatc cgcgccaggc gttcgtgagc
      721 cacgcggtca ctcagcagca tgggtttcag atagcgcata taaaccgtgc ggtcggaaag
      781 ggtttcgtgg aatttaacca acgccggctc atcttcggcc tgaatgggac ggatggtcac
      841 cggagagcca tcttttgccg tccactggct gatgtactgg ctgggatatg gacggatcgc
      901 cagacgaggc agcctgtctt cggtaacctc cggatcgtgg ataaccacgc gcgcatccag
      961 tgcgagcagg cgatcttcgg aagccaggag agggttgata tccagctcct tgatccagcg
     1021 ctgctcggca atcagctggc tgaagcgcac cagcatcagc tccagggcgg gcaggtcgac
     1081 tgatttgcgc ccgcgcacac ctctgagggc tgtgtaaatc gtcgtccgtt ccatcatgcg
     1141 gcgggcaagg gtagtattaa ggggtgggag ggccagggcg cggtctttga agacctcgac
     1201 cagcgtgccg cccatcccaa acaacaggac tggaccgaac tgcggatcaa tgctgctccc
     1261 gaggatcagc tcgtagccac ccagaggcag catgggctgg accgtgacac ccaggaaatg
     1321 ttccgcccca gctttttcgc tgaccgattt gaaaatgttc tcgaaggcgg cgcgcaccgc
     1381 gttcgcatcg cgcaggttta gcttcacccc gccgacatct gtcttgtgcg tgatggtctc
     1441 ggaatgcagc ttaaccacga tcgggtagcc aagctcgtca gcgattttca cggcttcatc
     1501 cgctgattta gcaatccggg tttctaccgt ggggatgccg taggcagaga gaagctgctt
     1561 ggattcgtat tctgtgagga tcgtacgccc ggtctggcgg acatgctgga taatctcagc
     1621 agcatgctca cgcctgggtt ctacatcgcc atcggggagc atgggggttt catacagcag
     1681 gcgcaggaaa gtcgagtagt gagccatata atcaaacatg cgggcggctg aatcggggta
     1741 ggcgaaggtg gggatattcg cctggttcag gatcatatcg cccttttcga catctgcgcc
     1801 gcccatccag ctcgccagga ccggcttgcc taaattatgc gcgtagggca ccagcttttc
     1861 agcggtggcg gtgggatccg tcattgcctg gggggtcagg attaccagga taccatcgct
     1921 attggtatca ttggcggcaa tttccagggc ggctgcgtat cgatccgggc tggcatcccc
     1981 aataatatct accgggttgt tgtggctcca ggttggtggc aatatttgat tgtaggcctg
     2041 catcgtttcg tccgaaagct cggtaagctg accgccattg gtgatcaagg catccgtagc
     2101 cagca
//
LOCUS       DATMXK010000300         2423 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_162183, whole genome shotgun sequence.
ACCESSION   DATMXK010000300 DATMXK010000000
VERSION     DATMXK010000300.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2423)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2423)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2423
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162183"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..477
                     /locus_tag="VN363_05530"
     CDS             <1..477
                     /locus_tag="VN363_05530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0070064 - proline-rich region binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SH3 domain-containing protein"
                     /protein_id="HWQ84005.1"
                     /translation="VFGYTFGAVVTLVGVILLIGISGTGAAFSAQIALPTQVPTSTLT
                     PTLTFTVTATSAPPTATLTPSLTPTPTLAPTETLTPTPTPVLALVRTRDGQGALIRSE
                     PGGQVIGSYLDNVMIQVLPERFEDNSGVWVKVIGPDGAQGWMLQSLLVTATPAPNW"
     gene            complement(479..913)
                     /gene="cdd"
                     /locus_tag="VN363_05535"
     CDS             complement(479..913)
                     /gene="cdd"
                     /locus_tag="VN363_05535"
                     /EC_number="3.5.4.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559289.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004126 - cytidine deaminase activity
                     [Evidence IEA];
                     GO_process: GO:0008655 - pyrimidine-containing compound
                     salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytidine deaminase"
                     /protein_id="HWQ84006.1"
                     /translation="MEKKNGMLTDELREKLIQVAVEARHWAYAPYSNYRVGAALLTSS
                     GKIYDGVNVENAAYPDSICAERVAIFKAVSEGETSFDALVVSTSNGGSPCGSCRQVLA
                     EFGLNTLVLIADDQGHVLCELRTDELLPGSFGPRDLLGKSQD"
     gene            complement(882..2159)
                     /locus_tag="VN363_05540"
     CDS             complement(882..2159)
                     /locus_tag="VN363_05540"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWQ84007.1"
                     /translation="MNEDIPLTVIFVVFLALDLALIVVRAAYLNTNHARLLSLRSQME
                     RPVNRALQVLLQDHRLEASLNAFLLIIHIVLASLAAIWLSRILTGPLIVSGVILAAGI
                     VFIWIEAGIERLVAKSADIWAVRLAWLAEAVQGTAGILIWPLGLSNEPPEGIDPSGNV
                     TETELMTLVDAGREEGIFEQVEQEMIQSVIQLHDTLAREIMVPRIDMLALDVDTPLEE
                     AVDAFLRSGHSRAPVFEETVDNTLGLMYAKDLLRVWREGNPAGSLRDLLRPAYFVPEA
                     KRVDELLTEMQDRSIHMAIVVDEYGGVAGLVTMEDIVEEIVGEIRDEYDQSEEAPFQT
                     LNESDTIFQGRVHLDDFNEIMLSNLPSEEADTIGGFIYSRLGRVPMVGETVEEAGLVL
                     TVEQVSGRRIRKVRAHRVPDEQADGEEKRHVNG"
     gene            complement(2156..>2423)
                     /locus_tag="VN363_05545"
     CDS             complement(2156..>2423)
                     /locus_tag="VN363_05545"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559291.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DapH/DapD/GlmU-related protein"
                     /protein_id="HWQ84008.1"
                     /translation="HFSYLGDATIAADVNIGAGTITCNFDGIHKNPTIIEEGAFIGSD
                     TMLVAPVKIGEGARTGAGSVVTKDIPPYTLAVGVPARSIRKLEK"
BASE COUNT          530 a          757 c          593 g          543 t
ORIGIN      
        1 gtatttgggt atacttttgg ggcagtggtt acgctcgtcg gagtcatcct gctgatcggg
       61 atcagcggca ctggggcagc cttcagcgct cagatcgcct tgccaaccca ggttcccact
      121 tccaccttaa cccccaccct gacctttact gtaacagcta cctcagcacc tccaacagcc
      181 acgctcaccc cctctctcac acccacgccg accctggcgc cgaccgagac cctgaccccc
      241 accccgacgc ccgtgctggc tttggtgcgc actcgcgatg ggcagggcgc gctcatccgt
      301 tccgagccag gcggacaggt tatcggcagc tatctcgata atgtcatgat ccaggtgctg
      361 cccgagcgtt ttgaagataa ttccggcgtg tgggttaaag tgattggtcc ggatggcgcg
      421 cagggctgga tgctgcaaag cctgctggtc accgccaccc cggccccaaa ctggtagatt
      481 aatcctgact tttccccagc aaatcccgcg gtccgaacga acctggaagc agctcatctg
      541 tacgcagctc gcacagcaca tgcccctgat catcggcgat caggaccagg gtattcaagc
      601 caaattctgc caatacctgg cggcacgacc cgcagggaga gccaccgttt gaggtggata
      661 ctaccagcgc atcaaagctc gtttcgcctt cagataccgc tttaaagatt gccacccttt
      721 cagcgcagat actatcgggg taagccgcat tttcaacatt caccccgtcg tatattttcc
      781 ctgaagaggt cagtaatgct gctcccaccc gatagtttga gtaaggggca tacgcccaat
      841 ggcgcgcctc aactgctacc tggatcaatt tttctcgcag ctcatccgtt aacatgccgt
      901 ttttcttctc catctgcctg ttcgtctggc acacggtggg cgcgcacctt gcgtattcgc
      961 cgcccgctga cctgttccac agtcagaacc aacccggcct cttcaacagt ctcaccaacc
     1021 atcggtacgc gacctaaacg actgtagata aacccgccaa tggtatcggc ttcttcactg
     1081 ggtagattgc tgagcatgat ctcattgaaa tcatccaggt gtactcttcc ctggaaaatt
     1141 gtatcgcttt cgttaagcgt ctggaaaggc gcttcttcac tctgatcata ttcgtcacgg
     1201 atctcaccta caatttcttc gacgatatct tccatcgtca ccagacctgc aacgccgccg
     1261 tattcatcaa cgacaatcgc catatggatg ctgcggtcct gcatttccgt cagcagctca
     1321 tccacccgtt tcgcctcggg tacgaaatag gccgggcgca gcaaatcgcg cagggagccg
     1381 gctggattgc cctcccgcca tactcgcagc aaatccttgg catacatcag gcccagggtg
     1441 ttatcgaccg tttcctcaaa gacaggcgcc cgtgagtgac ccgagcgcag gaatgcatcc
     1501 acagcttctt ccagcggggt atccacatcc agcgccagca tatcaatgcg cggcaccata
     1561 atctcgcgcg ccagtgtatc gtgcagctgg atgacggact ggatcatctc ctgttcaacc
     1621 tgctcgaaga tgccttcttc acggcccgca tcgaccaggg tcatcaattc ggtttcggtg
     1681 acatttccgg aaggatcaat cccttcgggg ggttcgttcg ataagcccag cggccagatt
     1741 aatattccgg ctgtcccttg cacagcttca gccaaccatg ccagccgtac cgcccagata
     1801 tccgcgcttt ttgccaccag gcgctcgata ccagcttcga tccagatgaa aactatccct
     1861 gcagccagga taactcctga cacgataagc ggcccggtca ggattcggct gagccagatg
     1921 gcagccagac tggccaggac aatatggata atcagcaaaa aggcgttcag gcttgcctcc
     1981 aggcgatgat cctgcagcag cacctgtaaa gcccggttta caggccgttc catttgcgag
     2041 cgtaaactca gcagcctggc atgattcgtg ttcaaatagg ccgcccggac cacaattaat
     2101 gccaggtcca gggctaaaaa gaccacaaaa atgacggtga gggggatatc ttcgttcatt
     2161 tctccagttt tcggatcgaa cgggctggca cacctactgc cagcgtataa gggggaatat
     2221 ccttggtgac caccgagcca gctccggtac gcgctccttc gccgattttc accggggcga
     2281 ccagcatggt atcgctgccg atgaacgcac cctcctcgat gatggttggg ttcttatgta
     2341 ttccgtcaaa attacaggta atggtacctg cgccaatatt gacatccgct gcaatggtgg
     2401 catcaccgag ataggagaaa tgc
//
LOCUS       DATMXK010000301         2236 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_105122, whole genome shotgun sequence.
ACCESSION   DATMXK010000301 DATMXK010000000
VERSION     DATMXK010000301.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2236)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2236)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2236
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105122"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            102..1196
                     /locus_tag="VN363_05550"
     CDS             102..1196
                     /locus_tag="VN363_05550"
                     /inference="COORDINATES: protein motif:HMM:NF014445.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016755 - aminoacyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0044038 - cell wall macromolecule
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="peptidoglycan bridge formation glycyltransferase
                     FemA/FemB family protein"
                     /protein_id="HWQ84009.1"
                     /translation="MNAVTWNHLIRSLPSPHVLQTWEWGDFKSRYGWQPIHQRWGGGA
                     GSREAAALILKRGLSRRLGKWLSVLYIPKGPLLDWHDQSTRATVFADLEQIARSQGAI
                     FIKIDPDVIIARGIPGEPSYQPDPLGEEVVGHLRACGWHFSQDQVQFRNTMEMDLSRS
                     EDELLVGMKQKTRYNIRLASRRGVEIRRGGLPDLPTLYQMYAETSVRDGFVIRSAMYY
                     LDLWSSFIQAGLAEPLIAAVAGTPVAGLLLFHFDQRAWYLYGMSSDQEREKMPNYLLQ
                     WEAIRQAKALGCRNYDLWGAPDEFSEQDSMWGVYRFKDGLGAQVVQHIGAWDLPVRPN
                     LYMLYTRVLPQILDRMRQRGTRQTRQVVGL"
     gene            1224..2198
                     /locus_tag="VN363_05555"
     CDS             1224..2198
                     /locus_tag="VN363_05555"
                     /EC_number="4.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016846 - carbon-sulfur lyase activity
                     [Evidence IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-cysteine desulfhydrase family protein"
                     /protein_id="HWQ84010.1"
                     /translation="MKPLPRIRIASLPTPVEKLPRLSAAVGGVVLSIKRDDQTGLAFG
                     GNKTRKLEYLVAEAQANGARTLITTGAIQSNHCRQTAAAAARFGFECILVLAGEAGLD
                     HGTTANLLLDQLLGAKIVWTSLTERDATLEAVFRDAWSAGKRPYLVPYGGSSPVGAAA
                     YAFALQELLEQKTHPDTIVVASSSGGTQAGLALGKDLFGFHGKILGISIDEPAGRLKP
                     RVANLANQIADLLDVKIHLDPDDIWVDDHYLGAGYGIPSPEEIEAVRLFARTEGILLD
                     PVYTGRAAAAMLDLIHRGAFQPGEQVLFWHTGGNPALFAGRYQEILTG"
BASE COUNT          470 a          597 c          665 g          504 t
ORIGIN      
        1 ctgctggacc ctgggacagg atctatcagc caattcttta ccgtgcttat caaatcctgg
       61 cccggcaaac tgccggattg ggttgagccg tgcctgatcg tatgaatgcc gtaacctgga
      121 atcacttgat ccggagcctg ccttcgccgc atgtcttgca aacctgggaa tggggcgatt
      181 ttaaatcccg ctatggctgg cagccgatcc accagcggtg ggggggcggc gccggcagcc
      241 gcgaagccgc ggcgctgatc ttgaagcgag gcttatcccg ccggttgggc aaatggctgt
      301 cggtgctgta tattccaaaa ggacctttat tggattggca cgaccaatct acaagggcta
      361 cggtatttgc tgacctggag cagattgccc gcagccaggg agcgatattt atcaagatcg
      421 accccgatgt gattattgcc cgggggattc ccggtgagcc ttcctaccag ccagacccct
      481 taggtgaaga agttgttggc catttacgtg cttgtggctg gcatttttcg caggatcagg
      541 tccagtttcg gaatacgatg gaaatggatc tatcccgctc tgaggatgaa ttattggtgg
      601 gcatgaaaca aaagacccgc tataacattc gtctggccag ccggaggggg gtggagatcc
      661 ggcggggggg attgcccgac ctgcccaccc tgtatcaaat gtatgctgag acctccgttc
      721 gggatggatt tgtaattcgc tcagcgatgt attatctgga tttgtggagc tcattcatcc
      781 aggccgggtt ggcagaaccc ttgatcgccg cagttgccgg cacacctgtc gcgggtttgc
      841 ttttattcca tttcgatcag cgcgcctggt acctgtacgg gatgtcgagc gatcaggaac
      901 gcgaaaaaat gcccaattac ctcttacagt gggaggcaat ccgccaggca aaggctctcg
      961 gctgcaggaa ctatgatttg tggggtgccc cggacgaatt cagcgagcag gattcgatgt
     1021 ggggtgttta ccggtttaag gatggtcttg gcgcccaggt tgtccagcat atcggggcct
     1081 gggatttacc cgtacgcccg aacctgtaca tgctttacac ccgggtcttg ccccagattt
     1141 tggaccggat gcgccagcgc ggcacccgcc agactcgcca ggtcgtcggc ctgtaatatt
     1201 ttattcatcc ctggaggcaa acaatgaaac cgttaccccg tatccggatt gccagtttac
     1261 ccacacctgt tgaaaagctg ccccggctat cggctgccgt tggtggcgta gtactctcga
     1321 tcaaacgcga tgatcagacc ggcctggcgt ttggaggtaa taaaacccgc aagctcgaat
     1381 acctggtcgc cgaagcccag gctaatgggg cgcggacatt aatcaccacc ggcgcaatac
     1441 agtccaacca ttgccgccag actgcggcgg cggcagcgcg cttcggtttt gaatgtatcc
     1501 tggtactggc tggcgaggcc ggtctggatc atggaacgac tgcaaacctg ctcctggacc
     1561 aactgctggg ggcaaagatc gtctggacca gcctcaccga gcgcgacgcc actctggagg
     1621 cagtgttcag ggatgcctgg agtgctggga aacgaccgta ccttgtccca tacggaggat
     1681 caagcccggt gggtgccgcg gcatatgctt ttgccctcca ggagctgctg gaacagaaga
     1741 cgcacccgga tacgatcgtg gtcgcctctt cttccggagg tactcaggcc gggttggccc
     1801 tgggtaagga tttatttggc tttcacggta aaatcctggg aatcagtatc gacgagccag
     1861 ccggcaggct taaaccccgg gtagcaaatc tggcgaacca gattgcagac ctcctggatg
     1921 taaaaatcca cctcgatcct gacgatatct gggtggacga ccattatctg ggggcaggat
     1981 atggcatacc gagtccagaa gagatcgagg ctgtgcgcct cttcgccaga acagaaggga
     2041 tactgcttga cccggtctat acgggacgcg cggctgctgc aatgctggat ttgatccaca
     2101 gaggagcttt ccagcctggc gagcaggtgc ttttctggca cacggggggt aatccggctt
     2161 tgttcgctgg gcgctaccag gaaattctca caggctgatt acaggcaggt taaatttgaa
     2221 atggcttctc aggaag
//
LOCUS       DATMXK010000302         3269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_57319, whole genome shotgun sequence.
ACCESSION   DATMXK010000302 DATMXK010000000
VERSION     DATMXK010000302.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57319"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..574
                     /locus_tag="VN363_05560"
     CDS             <1..574
                     /locus_tag="VN363_05560"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84011.1"
                     /translation="MEGKGQAQQDLEGSPEYHFEPYQPSPQIIDEYCLGIGASPELAE
                     RFHQQPLPAVQHLPTATRAVLGASHMQFIIPREVARQVTPLPATKALLRTETMSLDYK
                     IDQLGYLHLSTCLPYVFHMGNTINDRLLAEFQQITAQTSQNVNPRTNQSTSVSPLRRW
                     LARIARTPQGQRQLQRVYNLLFQVLHSDNR"
     gene            581..1444
                     /locus_tag="VN363_05565"
     CDS             581..1444
                     /locus_tag="VN363_05565"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWQ84012.1"
                     /translation="MIKPELLELCECTTCRGKYALQGDRLLCQECGETVPLLDSIPIF
                     TPVAGEIRPSEKLARGPDTGTPWRRANWQFLQNQLAALPGQARILDVGAGRGDFAALL
                     DRFQTIALDIYPYPEVNLVADLTQRNPFRPAQFDAVVLMNVLEHVYDTHTLLARLAEL
                     LKPGGILIIAIPFLVKLHQTPVDFVRYTHYALERFAGEHDLICETLEGYYDPLFFLSE
                     GTGNIKNAYLPAIPQFKRIWARLLLSGLQTFANGLQSLLGPGQARSPEEMRSQAPTGY
                     LLVYRKKLPEL"
     gene            1464..2897
                     /locus_tag="VN363_05570"
     CDS             1464..2897
                     /locus_tag="VN363_05570"
                     /inference="COORDINATES: protein motif:HMM:NF016357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0047355 - CDP-glycerol
                     glycerophosphotransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-glycerol glycerophosphotransferase family
                     protein"
                     /protein_id="HWQ84013.1"
                     /translation="MTSKTVFISADHGLAVFYFMQSQIIPILREAGVNVVVLTDDQSV
                     DLLCERYSEPGLIIEGLRLDQVQRYTRTVSPSTQWWLDFFRRAGAAPGANLAVVEHYI
                     RQVKGESHSRRRMLFPLMEGIARTMRTSPAARRMLQRTQMHYAPSIYGDLFEKYRPSL
                     VIAASPGFRQDRYLLREAKKRNIKTVAAIITWDSSSSYGLPGAPVDYITCWSEIQKQE
                     LVGGADWDPKRVHVGGMPPYDGYITDEWLMTRQDYYRRHNLDPRRKLLSYASSFVSWS
                     PNLQNVEALASLVTHDVLAEPTQLLVRLHPIHMSGHYVSEADAIRRLAAEHPHIHVVE
                     PVPLGALGHYSGEDMAEKTSMMAYSDIFLTVYSTMCVEASFQERPIVSVCIDSKTGWP
                     GKYWVPMSEIGVWPTHSRFRTSGAGKVALNVDELRQALNYYLRQPEASRDAQRRFLAQ
                     ECTYTDGSASRRTAEYFLNLLGKAHVN"
     gene            2887..>3269
                     /locus_tag="VN363_05575"
     CDS             2887..>3269
                     /locus_tag="VN363_05575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase"
                     /protein_id="HWQ84014.1"
                     /translation="MLTKTTQRLQSAWRVLLHGETPRSTGREVPAITQAEVAEARTFF
                     PAEKFFIYGHARSGTTLLTRLVRLHPEVHCNYQAHFFTRAPLLEGLVVAPDIASWLGR
                     NSNRWNHGRDLSPVVLRAVSDYIICL"
BASE COUNT          712 a          995 c          857 g          705 t
ORIGIN      
        1 gatggaggga aaaggccagg cacagcaaga cctggagggc agcccggaat atcactttga
       61 gccttaccag ccaagcccac agatcatcga tgaatactgc ctggggattg gggccagtcc
      121 agagcttgcg gagcgcttcc accagcagcc actgccggcc gtacaacacc tgccaaccgc
      181 aactcgggct gtgctgggcg cttcccatat gcagttcatc attcctcgag aagtagcccg
      241 ccaggtaaca ccgctcccgg cgaccaaagc cttgctgcgc accgaaacca tgtccctgga
      301 ttacaaaata gaccagctgg gctatctgca tctctccacc tgcctgccct atgtctttca
      361 tatgggcaat acgatcaatg accgcttgct ggcggaattc caacaaataa ccgctcagac
      421 cagccagaat gttaatccca ggaccaacca atccacatcc gtgtcacccc tgaggcgctg
      481 gctggccagg atagcgcgca cgccgcaggg acagcgccag ttacaacgcg tttacaatct
      541 cctgttccag gttctgcact cggacaatcg ctgagaaacc atgataaagc ctgaattact
      601 cgagctctgc gagtgtacaa cctgccgggg aaaatatgct ctccagggcg acagactgct
      661 ctgccaggaa tgtggagaga ccgtcccgct gctcgacagt atcccgatat ttaccccggt
      721 tgccggggag atccgcccat cggaaaaact ggcccgcgga cccgatactg gcactccctg
      781 gcgaagagcc aactggcagt ttctgcaaaa tcagctggca gccttgccgg gccaggcgcg
      841 catcctggat gtaggcgctg ggcggggcga ttttgctgcc ctgctggatc gcttccagac
      901 gatcgccctg gatatttatc cttaccccga ggttaacctg gtcgccgatc ttactcagcg
      961 caatcctttc cggcccgccc agtttgacgc cgtggtgctc atgaacgtac ttgaacacgt
     1021 ttacgatacg cacacgctgc tggctcgcct ggcggaactc ctaaaaccag gcggcatcct
     1081 gattattgct atccccttcc tggtgaaact ccaccaaacc ccagtggatt tcgtacgcta
     1141 cacccattat gcactggagc gatttgccgg cgagcacgac ctgatctgtg aaacgctcga
     1201 aggctattat gatcccctgt tctttctatc cgaagggacc ggaaatatca aaaacgctta
     1261 tctgccggca ataccccaat tcaaacggat ctgggcccgg ctgctcctga gtggcctcca
     1321 gacttttgcc aatggcttgc aatcgctgct cgggccagga caagcccgtt cccccgaaga
     1381 aatgcgcagc caggcgccta caggttatct gctggtgtat cgcaaaaaac ttcctgagct
     1441 ttaggagcca aacttaacgg attatgacct ctaaaactgt gtttatttct gccgatcatg
     1501 gcctggcggt gttctatttc atgcaaagcc agatcatccc catcctgcgc gaggccgggg
     1561 ttaatgtggt cgttctgacg gacgatcagt ctgtcgattt actctgcgag cggtattctg
     1621 aacctggctt gatcatcgag gggctgcgcc tggatcaggt gcagcgctat acacgcaccg
     1681 tttcgccttc cacccaatgg tggctggact ttttccgccg cgccggtgca gctccaggag
     1741 ccaacctggc agttgtcgaa cattacatcc ggcaggtaaa aggcgaatct cattcccggc
     1801 gccggatgct tttcccgttg atggaaggga tcgcccgtac catgcgcacc tccccggcag
     1861 cccgccggat gctgcagcgt acccagatgc actatgcacc gtcaatttat ggtgatcttt
     1921 tcgaaaaata ccggccttcc ctggtcattg ccgcttctcc gggtttccgc caggaccgct
     1981 atctactgcg ggaggccaaa aagcgaaata tcaaaaccgt cgcggcgatt atcacctggg
     2041 acagctcaag cagttatggc ctgcctggtg cgccggtaga ttacattacc tgctggtccg
     2101 agatccagaa acaggagctg gtgggcggcg cagattggga tccaaaacgc gttcacgtgg
     2161 gtgggatgcc gccctacgat ggctatatta ccgacgaatg gctgatgacc aggcaggatt
     2221 attatcgccg gcataacctg gatccgaggc gcaaattact gtcctatgcc agcagctttg
     2281 tcagctggtc gccaaacctt caaaacgtcg aggctctggc aagcctggtg actcatgatg
     2341 tactcgctga accaacccag ttactggtcc gcttgcatcc catccatatg agtggtcatt
     2401 acgtgagcga agctgacgcc atccggcgcc tggcagctga gcacccgcat attcatgtag
     2461 ttgagcctgt accattgggc gcattaggac attattctgg cgaagatatg gccgaaaaaa
     2521 catccatgat ggcctacagt gatatctttc tcaccgttta ttcgacaatg tgcgttgagg
     2581 cctccttcca ggagcgcccc atcgtgagcg tgtgcatcga ctcgaagaca ggctggcctg
     2641 gaaaatattg ggtgcccatg tccgaaatcg gagtctggcc cacccattcg cgcttccgca
     2701 catcaggcgc ggggaaagtg gcgcttaatg tcgatgaact tcgccaggct ctgaactatt
     2761 acctcaggca gccggaagcc agccgggacg ctcagcgccg cttcctggct caggaatgta
     2821 cctacaccga tggcagcgcc agccgccgga cggcagagta cttcctcaat ttgcttggga
     2881 aggcgcatgt taactaaaac cacccaacgc ctgcaatcag cctggagggt cctgctgcac
     2941 ggcgaaaccc cccgatcgac cggccgggag gttcctgcca ttacccaggc agaagtcgct
     3001 gaagccagaa ccttcttccc ggccgaaaaa tttttcatct acgggcatgc tcgctcgggc
     3061 accacgctgc tcacccgcct ggtgcgactg catccagaag tccactgtaa ttaccaggct
     3121 cactttttca cccgcgcccc tctcctggag ggtctggttg tggcccccga tattgccagc
     3181 tggctgggac gaaacagcaa tcgctggaat cacggtcgcg atctctcacc ggtggtgctg
     3241 cgcgcggtga gcgactatat tatctgtct
//
LOCUS       DATMXK010000303         2371 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_116397, whole genome shotgun sequence.
ACCESSION   DATMXK010000303 DATMXK010000000
VERSION     DATMXK010000303.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2371)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2371)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2371
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_116397"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..269
                     /locus_tag="VN363_05580"
     CDS             <1..269
                     /locus_tag="VN363_05580"
                     /inference="COORDINATES: protein motif:HMM:NF013422.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="translation initiation factor"
                     /protein_id="HWQ84015.1"
                     /translation="PRPKSLPPQQQVAAISRDRKGRGGKTVTLIRDLQLNPDDLKALL
                     KQLKQTCATGGTIKDGVIEIQGDHRTQIAEKLRTMGYKTKFVGG"
     gene            355..633
                     /locus_tag="VN363_05585"
     CDS             355..633
                     /locus_tag="VN363_05585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84016.1"
                     /translation="MSSSPEMQGRVLSLIMGMAAAMTPLGLIIAGPLADTLGVQSWFI
                     IGGAATILMAVSALFIPAIMNLEQGRKQTSFDTQPGLAVISEPLNPGD"
     gene            674..2059
                     /locus_tag="VN363_05590"
     CDS             674..2059
                     /locus_tag="VN363_05590"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ84017.1"
                     /translation="MAGVKTYQGMRTFLVIWFGQLISTLGSGLTGFALGVWIYQETGS
                     TTLFAMNMLAYAVPNLLVSPLAGALVDRWDRRVVMILSDTGAGLSTLVVFLLLVTNSL
                     EIWHVYIITAISAAFSTFQWPAYSAATSTLVPKEQLGRAGGLVQIGEAISQIIAPGVA
                     GALYVTAGLQGVILIDVLTFVFAVGSLLVVKIPRPEVSAEGEQGKGSLWKEATYGWKY
                     IIARPGLLGLLSVFAATNFFSGIMQPLLAPLVLDMTSADMLGYLGSVLGVGMLLGTLL
                     MSAWGGPRRKVHGVLGFLLIGGIFTSMVGLRPSIPLMTVSGFLLMLTFPIVNGSSQAI
                     WQSKVALDVQGRVFSVRRMIAWSTTPIAFAIAGPLADGVFKPLLLEGGGLAGSVGQII
                     GVGPGRGVGLMFILIGLFNVLVSAAGYLNPRIYHVEDELPDAVRPEDNEAETLSPSPE
                     TTVSVSGLSPE"
BASE COUNT          534 a          558 c          668 g          611 t
ORIGIN      
        1 gccccaggcc aaagagcctg ccgccgcagc agcaagtagc agcaatctcc cgggatcgta
       61 aagggcgggg tggaaaaacg gttactttga tccgtgattt acagcttaat ccagatgatc
      121 taaaagcctt actgaagcag cttaaacaaa cctgcgctac aggtgggacg ataaaagacg
      181 gagtcatcga aattcagggc gatcaccgga cacagatcgc cgaaaagctc aggacgatgg
      241 gttataagac taaattcgtt ggtggctagt caatcagcag gtgatccatc acctggaaaa
      301 cctcttcgaa ctcatcaaaa tcaaattatg ggcctttatt tgcagtactg caggatgtcg
      361 tcgtcccctg agatgcaggg tcgggtactg agcttgatta tgggtatggc agcagcaatg
      421 acacccctgg gattgattat tgccggaccg ctggctgaca cgttaggagt acaatcatgg
      481 ttcatcatcg gtggggcagc cacaattttg atggctgtaa gcgctttgtt tatcccggcg
      541 atcatgaatc tggagcaagg acgaaaacaa acttcgtttg atacgcagcc tggcctggct
      601 gttatatccg agccgcttaa tcccggcgac tgagcgagta aggcttccgt aactaccagg
      661 agtttatttg gtgatggctg gagtaaaaac ctatcaaggc atgcgtactt tcttagtaat
      721 ctggtttggc cagctaatct caacgcttgg ctctggatta accggctttg cattgggggt
      781 gtggatatac caggagacag ggtctacgac gctgtttgcc atgaacatgc tggcatatgc
      841 cgtacctaat ctgctggttt cccccctggc tggagcgttg gtcgatcgct gggaccggcg
      901 ggtggtaatg atcctgtcgg ataccggagc aggcttgagt actctggtgg tctttctgct
      961 gttggttaca aacagcctgg agatctggca tgtttacatc attaccgcca tcagcgccgc
     1021 cttcagcaca ttccaatggc cggcgtactc tgcagcaacc agcaccctgg tgcccaaaga
     1081 acagcttggc cgggcgggtg gattggtcca gatcggtgaa gcgatctcgc agatcatcgc
     1141 cccgggagtc gccggagcat tgtatgtgac ggctggctta cagggggtga tcctgatcga
     1201 cgtcttgact tttgtcttcg cggtgggctc tttattggtc gtcaagatcc ccaggccaga
     1261 agtgagcgcc gaaggggagc agggtaaagg ctcgctgtgg aaagaagcca cttacggatg
     1321 gaagtacatt attgctcgcc cgggtttatt aggcttgctg agtgtcttcg ctgccacgaa
     1381 tttcttctcc ggtattatgc agccgctcct ggcgcccctg gtattggaca tgaccagcgc
     1441 ggatatgctg ggctatctcg gttcggtgct tggggttggg atgctcctcg ggactttatt
     1501 gatgagcgcc tggggaggac ccaggcgtaa agtccacggt gtactgggtt ttctattgat
     1561 aggcggtata ttcaccagca tggtcgggtt gagaccttcc atccccttaa tgactgtgag
     1621 tggatttttg ttgatgctga cattcccgat cgttaatggc tcgagccagg caatctggca
     1681 gagcaaagtg gcgctggatg tacagggccg ggttttttct gtgcggcgga tgattgcctg
     1741 gtcaaccacc ccgatcgcgt tcgctattgc agggccctta gccgacggtg tgtttaagcc
     1801 actcctttta gaaggtggtg gattagccgg aagcgtgggt cagataatcg gggttggtcc
     1861 cggacgtggt gtgggattga tgtttatcct gatcggttta tttaatgtcc tggtttcggc
     1921 agccggctac cttaatcctc ggatctatca cgtggaagac gagcttcccg acgcagttcg
     1981 cccggaggat aatgaggcgg aaaccctttc accatcgccg gaaacaacgg taagtgtctc
     2041 tggcctatcc cctgaatgac cgggttcaag tgatgcaact aatttataga ttgtgcatct
     2101 aactcctttg aaacttgata acgtgcgcct gtatttggag cgcagaaaaa taaaatcaaa
     2161 ggagattggt atgccaatat atgaattcct atgtcaggaa tgtggttcac catttgaaga
     2221 gctcttgttc agctctgcgg cgatatccga gctgacttgc ccaaaatgtg gcagtgagaa
     2281 tattcgtaaa aaattgtcta cctttgcctc aaaaatatct ggaaagtccg gcatttcttt
     2341 tggaagtact gcctcatcct gcaacacagg c
//
LOCUS       DATMXK010000304         2186 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_85555, whole genome shotgun sequence.
ACCESSION   DATMXK010000304 DATMXK010000000
VERSION     DATMXK010000304.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2186)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2186)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2186
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85555"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            270..941
                     /locus_tag="VN363_05595"
     CDS             270..941
                     /locus_tag="VN363_05595"
                     /inference="COORDINATES: protein motif:HMM:TIGR00697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:1990397 - queuosine salvage [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="queuosine precursor transporter"
                     /protein_id="HWQ84018.1"
                     /translation="METQSHTADPVSPSRRAIQITRAGVLVIAAYIGAQMLSDIASLK
                     IGVVFGLAVDMGTFIYPITFTLRDLVHKLLGKRAAQTLILTAGGINLFMAGYLMWAAS
                     VPGSAAWGLNNEFAAILAPVWRIVLASIAAEVVSELIDTEVYHWFVTRITQRYQWLRV
                     LVSNGASVPVDNLIFAVGAFALTLPWTEVWQIFLFNLIIKYAMTLLSLPLIYLIPGKS
                     ETTVE"
     gene            complement(942..>2186)
                     /gene="glnA"
                     /locus_tag="VN363_05600"
     CDS             complement(942..>2186)
                     /gene="glnA"
                     /locus_tag="VN363_05600"
                     /EC_number="6.3.1.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR00653.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004356 - glutamine synthetase activity
                     [Evidence IEA];
                     GO_process: GO:0006542 - glutamine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type I glutamate--ammonia ligase"
                     /protein_id="HWQ84019.1"
                     /translation="VDMPVHRAQEAIEDGMWFDGSSVEGFARIQESDMRLVIDPATFV
                     VLPWTNPEYRRARVFCDIYTPEGKPFAGDPRSTLQSLLAKIEARGWTYNVGPEPEFFL
                     FRRNGERLTHPVPHDIGSYFDFSANDEAVTVRTELMDALLGMGLQVEMGHHEVALGQH
                     EIDFRFDNALVAADNILTLKYTVKAIAAQHGLVASFMPKPIYGINGSGMHCHQSLFDQ
                     QGKNLFFDKADQFNLSPLAYGFIAGQLAHARSFSAVVAPTINSYKRLVPGYEAPVYIG
                     WAQINRSALIRIPYYTAGRDNSIRAELRCPDPSANPYLALSVMLAAALDGIDRQLACP
                     EPLNNVNVYELTSEERHSVGVHELPGSLAEAMRELEKDSVIKDALGETIYEAFWRAKW
                     SEIEEARMKVTDWELERYLETA"
BASE COUNT          456 a          651 c          633 g          446 t
ORIGIN      
        1 caacgtcaag gtatacccca tcgcaaaaag cgggcaccag ccccagattg gtcaggctga
       61 gctggtcaac cgccgggtga tcgaatttct ccgcgccagc taaacaattt tccgcgtgat
      121 agcaggtata gatcagtaaa atcgggtaaa cttaaacatg gcttgatgcc ggtggcagcg
      181 tggaggcgca cagcgttctc ccacccggct taacaaaact gcggcggtcg cggccacccg
      241 cctcaacaaa atcaaggaga gccctgcact tggaaactca atcgcatacc gcagaccctg
      301 tctccccctc caggcgcgcc atccagatta cccgcgccgg agtgctggtc attgccgcct
      361 atatcggcgc ccagatgctc tcggatattg ccagcctgaa gatcggggtg gttttcggcc
      421 tggcagtcga tatgggcaca ttcatctacc cgatcacctt caccctgcgc gacctggtgc
      481 acaagctcct gggaaagcgc gccgctcaaa ctctgatcct gacggcgggc gggatcaacc
      541 tgttcatggc aggctatttg atgtgggcgg ccagcgtccc gggtagtgca gcctgggggt
      601 taaataacga gttcgctgcc atcctggcgc cggtgtggcg gatcgtgctc gcctccatcg
      661 ccgctgaagt agtcagcgaa ctcatcgata ccgaggtcta tcactggttc gttacccgca
      721 tcacccagcg ctaccagtgg ctgcgggtgc tggtcagcaa cggggccagt gtgcccgtgg
      781 ataacctgat tttcgcggta ggcgctttcg ccctgactct accgtggacg gaggtctggc
      841 agatcttctt gttcaacctg atcatcaaat acgcgatgac cctgctcagt ctgcccctga
      901 tctatctgat tccaggcaaa agcgagacca ctgtcgaata atcaggctgt ctcgaggtag
      961 cgctccaact cccagtcggt cactttcatg cgggcttctt cgatctccga ccatttcgca
     1021 cgccagaaag cctcgtagat cgtctcgccc agggcgtctt taatcacact gtctttttcc
     1081 agctcccgca tggcctctgc cagcgagccg ggcagctcgt ggacacccac agagtggcgc
     1141 tcttcgctgg tcagctcgta cacattgaca ttattgagcg gttccgggca agccagctgg
     1201 cgatcgatcc catccagggc cgctgccagc atcacagaca gcgccaggta cgggtttgca
     1261 gatggatcgg ggcagcgcag ctcggcgcgg atcgagttat cccgcccggc ggtgtaatag
     1321 gggatgcgga tcagcgccga ccggttgatc tgcgcccagc cgatatacac cggggcttcg
     1381 taaccaggca ccagacgttt gtaagaattg atcgtggggg caacgaccgc tgaaaaactg
     1441 cgcgcatggg ctaattgacc ggcaataaaa ccatacgcca gcggtgagag gttgaattgg
     1501 tcagctttgt cgaagaacag gttcttgccc tgctggtcga acaaagattg gtgacagtgc
     1561 atgccggagc cattgatgcc gtaaatcggc ttgggcatga aggaagccac cagcccatgc
     1621 tgggcggcga tggctttgac cgtatatttc agggtcagga tattatcggc ggcgaccagg
     1681 gcattgtcaa agcggaagtc gatctcgtgc tggcccagag cgacctcatg gtgccccatc
     1741 tcaacctgca agcccatgcc cagtaaagca tccatcaact cggtgcgcac ggtaacggcc
     1801 tcatcgttgg ccgagaaatc gaaatagctg ccgatatcat ggggaaccgg gtgggtcagg
     1861 cgctcgccgt tgcgccggaa gaggaagaat tctggctcag ggccgacatt gtaggtccag
     1921 ccgcgcgcct cgatctttgc cagcaggctt tgcagggtgc tgcgcgggtc tccggcgaag
     1981 ggtttcccct cgggagtata gatatcgcag aatacccgcg cccggcggta ctccgggttg
     2041 gtccagggca ggactacaaa ggtggcagga tcgatcacca ggcgcatatc gctctcctgg
     2101 atgcgggcaa agccttccac agatgagccg tcgaaccaca taccatcctc aatggcttct
     2161 tgagcgcggt gcaccggcat atccac
//
LOCUS       DATMXK010000305         2715 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_82041, whole genome shotgun sequence.
ACCESSION   DATMXK010000305 DATMXK010000000
VERSION     DATMXK010000305.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2715)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2715)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2715
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_82041"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            48..1157
                     /gene="serC"
                     /locus_tag="VN363_05605"
     CDS             48..1157
                     /gene="serC"
                     /locus_tag="VN363_05605"
                     /EC_number="2.6.1.52"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561429.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0004648 -
                     O-phospho-L-serine:2-oxoglutarate aminotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006564 - L-serine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3-phosphoserine/phosphohydroxythreonine
                     transaminase"
                     /protein_id="HWQ84020.1"
                     /translation="MTDLTQASHRVHNFSAGPATLPLPVLQTAQAEMLDYRGSGMSVL
                     EMSHRSEPFEAILAQAEANLRRLLDIPPQYKVLFQQGGASLQFAMVPLNLRPTGASAD
                     YLINGAWGKAAFKDARRLGNARSAATTEAENFNCTSAPSDLDLDPQAAYLHYTSNETI
                     HGVQYQQAPLSLAGVPLVCDMSSDFLSQPVDIARYGLIYAGAQKNAGPAGVTMVILHE
                     DLLERVPDTLPVMLDYRVQAAGGSMHNTPPTFAIYLVGLVLEWLRELGGLPAIARQNQ
                     MKADLLYTTIDQSGGFYRGHAQLDSRSLMNVTFRLPTEDLEKQFASSADMNGLVGLKG
                     HRSIGGLRASIYNACPLEAVQALAQFMREFQRQNG"
     gene            1192..2133
                     /locus_tag="VN363_05610"
     CDS             1192..2133
                     /locus_tag="VN363_05610"
                     /inference="COORDINATES: protein motif:HMM:NF012607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxyacid dehydrogenase"
                     /protein_id="HWQ84021.1"
                     /translation="MAQFQILVSDGLDDEGLALLRQGGEVRFKPQISAEDLQVEIGDC
                     DALVVRSRTRVTRAVIQAGKQLKVIGRAGVGVDNIDLSAAAGAGIIVVNSPTAATRAV
                     AEMALAFMLALARQLPAQDASMKMGKWEKSAYLGSELNGKTLGIVGLGRIGSQLALYA
                     RAIGMHVLAYHPDQSEAEVARLGAQITGFDELLAGSDYLSIHTPLNGETRNLLGRAEF
                     ARMKPGVRLINTARGGIVDEVALCEALDSGHVAGAALDVFASEPVVPGSIASHPRVIA
                     TPHIAAQTEEAQARAGVAIAEEVLAALQGQPLRWRVA"
     gene            complement(2220..2414)
                     /locus_tag="VN363_05615"
     CDS             complement(2220..2414)
                     /locus_tag="VN363_05615"
                     /inference="COORDINATES: protein motif:HMM:NF015957.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0080135 - regulation of cellular response
                     to stress [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PspC domain-containing protein"
                     /protein_id="HWQ84022.1"
                     /translation="MTQDKPKLRRSKRNRVFMGVCGGLAEFFGVSAFWFRLAFLLALI
                     PGGVPGLVTYLLMWIIIPSE"
     gene            complement(2530..>2715)
                     /locus_tag="VN363_05620"
     CDS             complement(2530..>2715)
                     /locus_tag="VN363_05620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84023.1"
                     /translation="GSCPASIYEPDKARQILGFPAELWLRIALSFGYPADPETIFRPP
                     HKGGRVDIDQIVHWNHW"
BASE COUNT          605 a          732 c          787 g          591 t
ORIGIN      
        1 gtataaggga tagacaatta agggtaaaga caataaggat ggatttaatg acagatctga
       61 cccaggcaag ccatcgcgtg cataattttt ccgccggtcc ggcgaccttg ccgctcccgg
      121 ttttacaaac ggcccaggcc gaaatgctcg actatcgcgg cagcggtatg tctgtcttag
      181 agatgagcca ccgctcagag cctttcgaag cgattctggc ccaggcagaa gccaatctgc
      241 gccggctcct ggatatcccg ccgcagtaca aagtcctgtt ccaacaaggc ggtgccagcc
      301 tgcagttcgc catggtcccg ctcaacctgc gtccaaccgg cgcctctgcg gattacttga
      361 tcaatggcgc ctgggggaag gctgctttca aggatgccag acggttgggc aacgctcgca
      421 gcgctgcgac gacggaagct gaaaatttca actgcacctc tgccccgtct gatctggatc
      481 tggatcccca ggcagcttat ctgcattaca catcaaatga gaccattcac ggagttcaat
      541 accagcaagc tccgctctcc ctggctggtg tcccattagt gtgcgatatg tcatccgatt
      601 ttctgagcca gccggtagat attgccaggt acggtttgat ctacgccggc gcccagaaaa
      661 atgcggggcc ggcgggggta acgatggtta tcctgcatga agatctgctc gagcgcgttc
      721 ctgataccct cccggtcatg ctcgattatc gcgtccaggc agccggaggc tcgatgcata
      781 ataccccgcc gacattcgcc atctatctgg ttggccttgt cttggagtgg ttgcgcgagc
      841 tgggaggctt gcccgcgatt gcccgccaga atcaaatgaa agctgacctc ctctatacca
      901 cgatcgatca aagcggggga ttctaccgcg ggcatgccca attggacagc cgctcgctga
      961 tgaatgtaac tttccggctg cccactgaag atctggaaaa gcaatttgcc agctccgccg
     1021 acatgaatgg tctggtaggt ttaaagggtc atcgctcgat cggcggtttg cgggcatcca
     1081 tctataatgc ctgcccgctt gaggccgttc aagcgctggc ccaattcatg cgggaatttc
     1141 aaagacagaa cggctaggtt tgatgatcaa gggaaggctg taatcacgtc catggcacaa
     1201 tttcagatcc tggtctcaga tggcttagat gatgaaggcc tggcgctgct gcgccagggt
     1261 ggtgaagttc gctttaaacc ccaaatttcc gctgaagatt tacaggtcga gatcggcgac
     1321 tgcgatgctc tggtggtgcg ctcacgcacc cgggtcacca gggcagttat ccaggcgggt
     1381 aaacagctta aagtgatcgg ccgggccggg gtgggggtcg ataatattga tttatccgcg
     1441 gctgccgggg ccgggattat tgtggtcaac agcccgacgg ctgccacccg agccgtcgcc
     1501 gagatggctt tagcctttat gttggcgctg gcacgccagc tgccagccca ggacgcttcc
     1561 atgaaaatgg gcaagtggga aaagagcgcc tatctgggaa gcgaattaaa tgggaaaacc
     1621 ctgggtatcg tgggcctggg caggatcgga tcgcagctgg ccctgtatgc cagggctatc
     1681 gggatgcacg ttctggcata ccaccctgat caatctgaag cagaagttgc ccggctggga
     1741 gcgcaaataa ctggctttga tgagctcctg gctggctctg actacctgtc aatccatacg
     1801 ccgctgaatg gcgaaacccg gaatttatta gggcgggctg agtttgccag aatgaagccc
     1861 ggggtgcgcc tgatcaatac cgcccggggt ggcatcgtag acgaggttgc gctttgtgaa
     1921 gccctggatt cgggacacgt tgccggggca gccctggacg tatttgcttc cgagccggtg
     1981 gtgccgggaa gtatagccag ccatccccgg gtgatcgcca ccccccatat tgctgctcaa
     2041 actgaagagg ctcaggctcg ggcaggtgtg gcgattgctg aggaagtact ggctgcgctg
     2101 caaggccagc ccttgcgctg gagagtggcc tgaagcgttg ccttcaataa tcaaaacaag
     2161 ggttgccccg gagggcaacc ctgtgctgct tgctggctca cttctgttac tgggggaaat
     2221 tattctgatg ggatgataat ccacatcaag agataagtca ccagacccgg aacaccgcca
     2281 ggaatgagcg ccagtaagaa cgccagacgg aaccagaatg cgctgacgcc aaaaaattca
     2341 gccaaacccc cacagactcc cataaataca cgattccgct ttgaccggcg taatttcggt
     2401 ttatcttgtg tcattgttta cctcccggtt gttactgcga atacaggcaa atttgaacta
     2461 ctgtttcaca atgatatacg caggaagggg cttcgggttg cgtaaagata atcgagtccg
     2521 tccggtggct taccagtgat tccaatgcac aatctgatcg atatcgacac gcccaccttt
     2581 atgaggaggg cggaagattg tttctggatc ggcgggataa ccaaaggaga gcgcaatccg
     2641 cagccacagc tcagcgggga atccgagtat ctggcgggct ttgtccggct cataaatgct
     2701 ggccgggcag gagcc
//
LOCUS       DATMXK010000306         4723 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_84490, whole genome shotgun sequence.
ACCESSION   DATMXK010000306 DATMXK010000000
VERSION     DATMXK010000306.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4723)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4723)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4723
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84490"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..242
                     /gene="rfbC"
                     /locus_tag="VN363_05625"
     CDS             <1..242
                     /gene="rfbC"
                     /locus_tag="VN363_05625"
                     /EC_number="5.1.3.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_001100785.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008830 - dTDP-4-dehydrorhamnose
                     3,5-epimerase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose 3,5-epimerase"
                     /protein_id="HWQ84024.1"
                     /translation="ENKHQLWVPPGFGHGFYVLSEWAEFYYKVTDFYAPQWERTILWD
                     DPTLDITWPLIANQPVLLSPKDAQGQRMAEAELYE"
     gene            264..1337
                     /gene="rfbB"
                     /locus_tag="VN363_05630"
     CDS             264..1337
                     /gene="rfbB"
                     /locus_tag="VN363_05630"
                     /EC_number="4.2.1.46"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561304.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008460 - dTDP-glucose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-glucose 4,6-dehydratase"
                     /protein_id="HWQ84025.1"
                     /translation="MQNVLVTGGAGFIGSNFVRYLLAAEPAVQVVNLDLLTYAGSLEN
                     LKDLPDPARHTFIQGNICDRPLVDELLHKHQIDTLVHFAAESHVDRSILDPQSFIQTN
                     IIGTHTLLEACRAYWLVENKAGAQDVRFHHISTDEVFGTLAPDEPAFEETTPYAPRSP
                     YAASKASSDHLVRAYWTTYGLPYTLTNCSNNYGPYQFPEKVIPLMIINALQGKKLPIY
                     GDGKQIRDWLYVEDHCEAIWTVLKRGEPGESYNVGGDNQPTNLTVVNTICEILDECLP
                     ASPYMPHASLIQHVTDRPGHDRRYDVNIARIQRELGWQPSQSLTSGLLKTVEWYLNHP
                     EWIDAIHQQQDYQSWLNKNYTQR"
     gene            1360..2253
                     /gene="rfbA"
                     /locus_tag="VN363_05635"
     CDS             1360..2253
                     /gene="rfbA"
                     /locus_tag="VN363_05635"
                     /EC_number="2.7.7.24"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561303.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008879 - glucose-1-phosphate
                     thymidylyltransferase activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate thymidylyltransferase RfbA"
                     /protein_id="HWQ84026.1"
                     /translation="MKGIILAGGKGSRLYPLTTAISKQLLPVYDKPMIYYPLSMLMLA
                     GIREILIISSPEAIPAFRVLLGDGSQWGLHFEYAEQPSPRGLADAFLVRSEFIAGEPV
                     CLILGDNIFFGQGLRSKLRSAAQLQDGALIFAYPVRDPERYGVVEFDASGKALSIEEK
                     PLHPRSHYAVPGLYFYDPKVVDIAANLKPSPRGELEITDVNRVYMEKGELKVDVIGRG
                     VAWLDAGTHESLLQAAMFVQAVEDRQGMMISCPEEIAFRMGYIDTDGLRRQAEIMQGN
                     QYGAYLLGLLQSNLSTHPEEA"
     gene            2257..3153
                     /gene="rfbD"
                     /locus_tag="VN363_05640"
     CDS             2257..3153
                     /gene="rfbD"
                     /locus_tag="VN363_05640"
                     /EC_number="1.1.1.133"
                     /inference="COORDINATES: protein motif:HMM:NF016228.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008831 - dTDP-4-dehydrorhamnose reductase
                     activity [Evidence IEA];
                     GO_process: GO:0009243 - O antigen biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019305 - dTDP-rhamnose biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTDP-4-dehydrorhamnose reductase"
                     /protein_id="HWQ84027.1"
                     /translation="MDRILLLGCTGQLGWELQRTLAPLGDVTGLDCPEIDLAQPDSIH
                     EVIRQIQPKIIVNATAYTAVDRAESEPELAMAINGIAPGLLAEAAVSMKATLIHFSTD
                     YVFDGCKGSPYIETDGPNPLSVYGSSKLAGEAAVNQAGGAYLILRTSWVYSVRRPSFV
                     TKVLEWSRKQAVLRMVTDQVANPTSARMLAEVTAQLLVKGFARGNNSLNDWIKDRSGI
                     YHLAGSGIASRLEWAQAILDLDPHAAEQVTKKIEPALTAEFPSPAQRPLYSALNCDLF
                     EQTFGLQLPDWRMALQLALDPG"
     gene            3175..3990
                     /locus_tag="VN363_05645"
     CDS             3175..3990
                     /locus_tag="VN363_05645"
                     /inference="COORDINATES: protein motif:HMM:NF013245.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84028.1"
                     /translation="MTYEYNSASLKPPFIREFLELWQYRDLLGLLVTNSIKTRYKRSS
                     LGILWTLLNPLLNTLVLSIAFQAIMRFDIENYTIYLLVGLLVWTFFSQTTMHSMNTLI
                     WGSNLLKRIYVPRTIFAVSVVGNGLINYLLSLIPLTVIMLFMRQPFTPALLLLPVAIL
                     LLAMFTLGISLFMSTLAVFFVDIVDIFGVLLSALFYLTPIIYPLSIIPERFLPLINLN
                     PMTVMVSMFRSLIYYGELPAMNVSLTAVALAFISLGIGWWMFTKRVDEFAYRI"
     gene            3974..>4723
                     /locus_tag="VN363_05650"
     CDS             3974..>4723
                     /locus_tag="VN363_05650"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84029.1"
                     /translation="MLTESKPGMIVHKSDPVIELSSVSVRYRVPQERIPSLKEYAIRW
                     LKGQINYQEFWALRSIDLDIQPGEVFGIIGPNGAGKSTLLKVVSRVLRPTQGNLRVRG
                     RISPLLELGAGFDPELTGRENVYLNGAILGYSKAEIAECFEEIVEFAGLPEFIDAPVR
                     TYSSGMYARLGFSVATMKRPEILIVDEILGVGDAEFQTKSYERIQRFQGEGTTILLVS
                     HSMERVKEICARAIWLDHGEIIARGSADQVVD"
BASE COUNT         1043 a         1341 c         1237 g         1102 t
ORIGIN      
        1 ccgaaaataa gcaccagctc tgggttcccc cgggctttgg gcatggcttt tacgtgctga
       61 gtgaatgggc cgaattctat tataaggtga ccgattttta cgctccccaa tgggaacgaa
      121 ccatcctctg ggatgatcca accctggaca ttacctggcc tttgatcgcc aatcagccag
      181 ttctgttatc tcctaaagat gcccagggcc agcgcatggc cgaggcggag ctttatgaat
      241 agccacaatc cacgggaggc ggcatgcaaa atgtactggt aacgggtgga gccggtttta
      301 tcggctcaaa ctttgtgcgc tacctcctgg cagccgagcc agcagtccag gtcgttaatc
      361 tggaccttct gacctatgcc ggcagcctgg aaaatcttaa agatctgccc gatccggcgc
      421 ggcacacctt catacagggg aatatttgcg accggccatt ggtagatgag ctcttacaca
      481 aacatcagat cgatacgctc gtgcactttg ccgccgaatc gcatgtcgac cgctcaatcc
      541 ttgaccccca atccttcatc cagaccaaca ttatcggcac ccacaccctc ctggaagcct
      601 gccgggcgta ctggctggtc gaaaacaaag ccggcgctca ggacgtgcgt ttccaccata
      661 tttccaccga tgaagtcttc ggtaccctgg caccagatga acctgccttc gaagaaacca
      721 cgccttacgc cccacgctcg ccctacgccg catcgaaagc atccagcgat cacctggtgc
      781 gcgcctactg gacaacttac ggtctgccct acactctgac caactgctca aataattatg
      841 ggccgtacca gttcccggaa aaagtcatcc ccttgatgat tatcaacgcc ctgcaaggca
      901 agaagctgcc tatctatgga gatggaaaac agatccgcga ctggctgtat gttgaagatc
      961 actgcgaggc gatctggacg gttctgaagc ggggtgagcc aggcgaatca tataatgtgg
     1021 gtggtgacaa ccagcccacc aacctgactg tcgtgaatac aatctgcgag atcctcgatg
     1081 agtgcctgcc cgcctctccc tatatgccgc atgccagctt gattcagcac gtcactgacc
     1141 gtcccgggca cgaccggcgc tacgatgtca atatcgccag gatccagcgc gaattaggct
     1201 ggcagccgag ccaatctttg acctcaggtt tgttgaaaac cgtggaatgg tatctcaatc
     1261 accctgagtg gattgatgcc atccaccagc agcaggatta ccagagctgg ctgaacaaga
     1321 attacaccca acgctgaaac tggaagcagg aggggagcaa tgaaaggaat tatcctggca
     1381 ggcggtaaag gcagccggct gtacccgtta accaccgcca tcagcaaaca gctgctgccg
     1441 gtttacgaca aaccaatgat ctattatccg ctctcgatgc tcatgctggc aggcattcgc
     1501 gagatcctga tcatcagctc cccggaggca atccctgcat ttcgggtttt gcttggcgat
     1561 ggcagccagt ggggcttgca ttttgaatat gcggaacaac ccagcccgcg cggcctggca
     1621 gatgcattcc tggtacgctc ggaattcatc gctggtgagc ctgtatgctt gatcctgggg
     1681 gataacatat tctttgggca ggggctccgc tccaagctgc gcagtgctgc tcagctccag
     1741 gacggagcgc tgatcttcgc ttatcctgtg cgcgacccgg agcgttacgg tgtcgtcgaa
     1801 ttcgacgcct ctgggaaagc cctgagcatc gaagagaaac ccctccaccc acgctcgcat
     1861 tacgccgtgc ccgggcttta tttttatgat ccgaaagtgg tcgatatcgc cgccaactta
     1921 aaaccctccc cgcgcggcga gctggaaatc accgatgtca accgggttta tatggagaaa
     1981 ggcgagctga aagttgatgt tattggccgc ggcgtcgcct ggctggatgc cggaacccac
     2041 gaatccctgc tgcaggctgc catgttcgtc caggcggtgg aagatcgcca gggcatgatg
     2101 atctcttgcc cggaagaaat cgccttccgc atgggttata ttgatacaga cggcttgcgc
     2161 cgccaggcgg aaatcatgca aggcaaccag tatggcgcct atttgctggg cctgctgcag
     2221 agcaaccttt ccactcaccc cgaggaagcc tgagtggtgg atcgaatatt attactgggg
     2281 tgcacgggtc agctgggctg ggagctgcag cgaaccctgg cgccgttggg cgatgtgacc
     2341 ggcctggact gtccggagat cgacctcgcc cagccagatt cgattcacga ggttatccgc
     2401 cagatccagc ccaaaattat cgtcaacgcc actgcttata ccgcggtaga ccgggccgag
     2461 agcgaacctg agctggcaat ggcgatcaat gggatcgcac caggtctgct cgccgaagcc
     2521 gcggtttcaa tgaaagccac cctgatccac ttttcaaccg attatgtatt tgatggctgc
     2581 aagggcagtc cctatattga aaccgatggg cccaatccgc tcagcgttta cggcagcagc
     2641 aagctggccg gcgaagcagc cgtcaaccag gctggagggg catatctcat attgcggacc
     2701 agttgggttt acagtgtccg ccgtccaagt tttgtcacca aggtattaga gtggtcacgc
     2761 aagcaagccg tactgcgcat ggtcaccgat caggttgcca acccgacctc ggcgcgcatg
     2821 ctggcagagg tgactgctca acttctcgtt aaaggctttg cccggggtaa taactcgctc
     2881 aatgattgga tcaaggaccg ctccggcatt taccatctcg ccgggagcgg gatcgccagc
     2941 cgcctcgaat gggctcaagc gatccttgat ctcgaccccc acgcggctga gcaggtaacc
     3001 aaaaaaattg agccggcgct taccgcagag tttccttcac ccgcccaaag accgctttac
     3061 tcagctctga attgcgatct ctttgagcaa acctttgggc tgcagctgcc agattggcga
     3121 atggcattac agctcgccct ggatccaggc taatctcaga caatcaggca ccctgtgact
     3181 tacgaatata actcagccag cttaaaacca ccctttatcc gcgaattcct ggagctctgg
     3241 cagtaccggg atttgctggg tttgctggtg accaatagca taaaaacgcg ctacaagcgc
     3301 tcttcactgg gcatcctgtg gactctgctc aacccactcc tgaataccct ggtgctgtcg
     3361 atcgcctttc aggcaattat gcgcttcgat atcgagaact acaccatcta tctgctggtc
     3421 ggattgttgg tgtggacatt tttctcccag acgacgatgc attctatgaa taccctgatc
     3481 tggggcagta atctattaaa gcgcatctat gtgccacgga ccatcttcgc cgtttccgtt
     3541 gtgggcaacg gcttgatcaa ttacctgctc tccttaattc ccctgacggt catcatgctc
     3601 ttcatgcgcc agcctttcac cccggccctg ctgctgctcc cggtagccat cttactcctg
     3661 gcaatgttta ctctgggcat ctccttattc atgtccaccc tggcggtatt ttttgtagat
     3721 atcgtggata tctttggggt cttgttgtcg gcactttttt acctgacccc gattatttac
     3781 cctctcagca ttattccaga acgattcttg cctttgatca acctgaaccc catgactgta
     3841 atggtcagca tgttccgctc tttgatttat tatggcgagc tgccggcaat gaacgtcagc
     3901 ctgaccgcag ttgctctggc atttatctct ttagggattg gttggtggat gtttaccaag
     3961 cgggtggatg aatttgctta ccgaatctaa gccgggcatg atcgtacaca aaagcgaccc
     4021 cgtgatcgag ctcagcagcg tctcggtgcg atatcgcgtc ccccaggagc ggattccttc
     4081 cctcaaagag tatgcaattc gctggttaaa aggtcagatt aattatcagg aattttgggc
     4141 gctgcgcagt attgatctgg atatccagcc cggggaagtc tttggcatta tcggaccaaa
     4201 tggagccggt aaaagcacct tattaaaggt cgtctcacgc gtcttgcgtc ctacacaagg
     4261 caacctgcgc gtgcggggtc gcatctcccc gctgcttgag ttgggcgccg ggttcgaccc
     4321 ggagcttacc ggccgtgaaa atgtctatct caacggcgca atcctgggtt attctaaggc
     4381 agagatcgcc gaatgcttcg aggagattgt cgaatttgcc ggcttgcctg agtttatcga
     4441 tgcacccgtc cggacatatt cgagcgggat gtatgctcgc ctgggattct ctgtcgcgac
     4501 aatgaaacga cctgagattt tgattgtgga tgagattctg ggtgttggcg acgctgaatt
     4561 ccagaccaaa tcctacgagc gcatccagag attccaggga gaaggcacga cgatcctgct
     4621 cgtctctcac agtatggagc gtgtcaaaga aatctgcgcc cgggcgatct ggctggatca
     4681 tggggaaatt attgcgcgcg gatctgccga ccaggttgtc gac
//
LOCUS       DATMXK010000307         2493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_160186, whole genome shotgun sequence.
ACCESSION   DATMXK010000307 DATMXK010000000
VERSION     DATMXK010000307.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2493
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160186"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1572
                     /locus_tag="VN363_05655"
     CDS             <1..1572
                     /locus_tag="VN363_05655"
                     /inference="COORDINATES: protein motif:HMM:NF019318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HDIG domain-containing protein"
                     /protein_id="HWQ84030.1"
                     /translation="PNSFYSEELTNAARLTAAETVEPVSRTVIAGERIVQRGQVLNSA
                     DLEALQMLGFQQPQRQWQELVSAATLALLAGAFFTFFFRRQSPKITNSRGLWVIVGLF
                     LAFLFAGRLSLPGHIVLPYAFPLATYGLLISILFGFKISVVTSLPLAILIVFGMTNDM
                     ELLAYHILGTLFGMLAIGGARRLTAFFSAGTAIALSGVLVVLAYRLPVPSTDWAGLAT
                     LSGAALFNGLAATSLTLVLQFFLAQFLGLTTPMQLTELTRPDHPLLQSIMRDAPGTYQ
                     HSLQVANLAEQAAERIGADPLLTRVGALYHDAGKTQASMYFIENQLPGFANPHESLDP
                     QSSSTIIIRHVTDGVELLRRYHLPKRLWDFILEHHGTTITNYQYVRAVEAAGGDESLI
                     NRDDYRYPGPRPQSRETAILMLADTCEAAVRAERPADEQALQRIIHGTVENRIATGQL
                     DDTNLTLRDLATIADSFESTLRGIYHPRMIYPKLENIKPPQLPAAADVPTLTRGQVEE
                     RAAADATVNQSLKEV"
     gene            1569..2033
                     /gene="ybeY"
                     /locus_tag="VN363_05660"
     CDS             1569..2033
                     /gene="ybeY"
                     /locus_tag="VN363_05660"
                     /inference="COORDINATES: protein motif:HMM:TIGR00043.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004540 - RNA nuclease activity [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0042254 - ribosome biogenesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rRNA maturation RNase YbeY"
                     /protein_id="HWQ84031.1"
                     /translation="MINIQVAEEIELAEELTGLNDELERAARTALELAGAEPAAGLTI
                     VLTDDAQIQQLNSQFSGLDAPTDVLSFPAGELDLDSDELYLGDIVISAPRALAQASAG
                     GHLYREEMQLLVVHGVLHLLGHDHGEPDEKQRMWALQSQALAALGCTISSPP"
     gene            2081..2467
                     /locus_tag="VN363_05665"
     CDS             2081..2467
                     /locus_tag="VN363_05665"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013391.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWQ84032.1"
                     /translation="MVSFLRSRGRSFRYAFAGWRHALRTQPNTWIHATATLAVGLLAW
                     WLDLDTLEWAVLVLVIALVWTAELINTALEAAVDLANPQQHALAQVAKDAGAGAVLAA
                     ALGSVVIGLLILGPPLWARIQSILTP"
BASE COUNT          504 a          691 c          742 g          556 t
ORIGIN      
        1 cccaacagtt tttacagcga ggaactgacc aatgccgccc gcctgacagc tgctgaaact
       61 gttgagccag tcagccgcac cgttattgcc ggtgagcgca tcgtgcagcg tgggcaggta
      121 ttaaattccg ccgatctgga agctctacaa atgctgggat tccagcagcc acagcgccag
      181 tggcaggagc tggtcagtgc ggcgaccctg gctttattgg ccggggcgtt cttcaccttc
      241 tttttccgcc gccagagtcc gaaaatcacg aattcccgcg gcctgtgggt catcgtgggc
      301 ctattcctgg catttttatt cgccgggcga ctgtctttgc cgggacatat cgtacttccc
      361 tatgcgtttc cactggcaac gtacggcctg ctgatttcga tcctgttcgg atttaaaatc
      421 tcggtggtta cctcgctgcc gctggcaatt ctgatcgtat tcgggatgac caacgacatg
      481 gagctgttag cttaccatat cctcggcacg ctgtttggca tgctggcgat cggaggcgcc
      541 cgccggctga ccgcattttt ctcggctgga acggcaatcg ccctgagcgg agtcctggtg
      601 gtcctggctt acagattacc tgtcccttcc accgattggg cagggttggc gacattatcg
      661 ggggcggcgc ttttcaatgg gctggcagct accagcctga ccctggtact ccaatttttc
      721 ctggcccaat tcctggggct gacaacgccg atgcagctca ccgagctgac tcgccccgac
      781 catcccctct tacagagcat catgcgggat gcacccggaa cctaccagca cagcctgcag
      841 gtcgccaacc tggctgaaca agctgcagag cggatcgggg ctgacccctt gctgacccgg
      901 gtcggggcgt tgtatcacga tgcaggcaag acccaggcgt cgatgtattt tatcgagaac
      961 cagctgcctg gcttcgctaa cccacatgaa agcctggacc cacagagcag ctcaacgatt
     1021 atcatccgcc atgtaaccga tggggttgaa ttactgcggc gctaccacct gccgaagcga
     1081 ttatgggatt tcattctgga gcaccatgga acgacgatca cgaattacca gtacgtacgg
     1141 gcggtagagg cagctggtgg agatgaaagc ctgataaatc gagatgatta ccgctatccg
     1201 gggccgcgac cgcaatctcg cgagactgcc atcctgatgc tggcagatac ctgtgaggca
     1261 gcggtacgcg ctgagcgccc ggcagatgag caagccttgc agcggattat ccacgggact
     1321 gttgaaaacc gcattgctac cgggcagctc gatgatacca atctgacttt gcgcgatctg
     1381 gccacaattg ccgattcgtt tgagtcgaca ctccgcggca tttatcatcc acggatgata
     1441 tatcctaaac ttgaaaacat aaaacctccg cagctgcctg cggctgcaga cgttcccacg
     1501 ctaacgcggg gacaggttga agagcgggcg gcagcggatg caacggtaaa tcaatctttg
     1561 aaggaagtat gatcaatatc caggtcgccg aagagataga attggccgag gagcttactg
     1621 ggctgaatga tgagcttgaa agggcagccc gaactgcgct ggagctggca ggagcggagc
     1681 cggcggccgg gctgacgatt gtgctaacgg atgacgccca aatccagcaa ttaaatagcc
     1741 agttttcagg tctggatgca ccgaccgacg ttctgtcttt tccggcgggg gagcttgatc
     1801 tcgactcgga cgagctgtat ctgggtgata tcgtgatctc ggcgccgcga gccctggcgc
     1861 aagccagcgc aggtgggcat ctctatcgtg aagagatgca gttgctggtt gtacacgggg
     1921 tgctccacct gttggggcat gatcatgggg agccggacga aaaacagcgg atgtgggcgc
     1981 tgcaaagcca ggcactggca gcgttgggtt gtacgatctc atcacctccc tagatgcgct
     2041 gattcgcgcc ctcaacttaa ttcttaagag gcattgagat ttggtctcat ttttgcgttc
     2101 tcgcgggcga tccttccggt atgcttttgc tggttggcgg catgcactgc gcacccagcc
     2161 gaatacctgg atccacgcca ctgctaccct ggcagtgggg ttgttggcct ggtggctgga
     2221 cctggatacg ttggaatggg cggtgttggt gttggtaatc gccctggtct ggacggctga
     2281 gcttatcaat actgcactgg aagcggcagt ggatctggca aacccgcagc agcacgcgct
     2341 ggcgcaggtg gctaaggatg ctggagcagg tgcagtgttg gcagccgcgc tgggctcggt
     2401 ggtcattggg ctgttgatcc ttggtccgcc cttgtgggcg cgaatccagt cgatcttgac
     2461 gccttgaagg cgaaactcat ccaccagaat ccc
//
LOCUS       DATMXK010000308         2102 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161884, whole genome shotgun sequence.
ACCESSION   DATMXK010000308 DATMXK010000000
VERSION     DATMXK010000308.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2102)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2102)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2102
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161884"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2102
                     /locus_tag="VN363_05670"
     CDS             <1..>2102
                     /locus_tag="VN363_05670"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ84033.1"
                     /translation="QALLDTGKTSPALTSAKLAATLAPADLQHQELLLNCLEANQEWP
                     TALEVRQQLLKRRPGTPDSADWHALAGCALQAQVPAEAISAAEKALKIDAQDGIAHAL
                     YGAALLQQNDAEGALSHFSTATELAPHKAEPWLALAEAYSHSNDPVRAFDTLRSGSLA
                     APHSARLQLALGKASLDAGAPSQALGAFRKSASLIQENNPEDQPIGSVYGMADLHHEI
                     AFYLGETLRQLGHLDEARQVFSAAYQETNQRKPSDTRLAHAYAKTLLGLNATAEAIRP
                     LQDVVAGTPADADPYVDLARSLLELGGPRRDYAAQSIEYLQQALAINPNYAEAQGLLG
                     EAMEANHQPTRALSAYQKALETALIDDPAWKARLQHGVGRAALQNGQAETALVSLREA
                     AQLEPLNPGIQQTLASAYLELGMYADSLNAARTALQLNPAQTQVMAWFAQHARSLLSA
                     SDGQINALPEAGKALAQAIQSDPSQIELYIDLGQLHLENSNQAAALRAFIEAADRSSQ
                     VSLSLAQVLLLARELRHLGEAEKAAILLTQSLQEYPGSDDYTPDQDRPETQLWYELSI
                     TQLQLGSQTAAIQALDKAILCQPDNPTLLVKKADLLVECDRLADASSLLQRLLSEAPE
                     DIALYIKAGFVLRGLGEYTTALAYAEQAISLVETGTPGSMAARAHSLAAQLGRSLLMP
                     DKALAWISSVDPDTDEQT"
BASE COUNT          442 a          725 c          591 g          344 t
ORIGIN      
        1 ctcaggcgct gctcgatacc ggtaaaacga gcccggcgct gacctcagcc aaactggcag
       61 ccacactggc gccagctgac ctgcagcacc aggagctgct gctgaactgc ctggaggcta
      121 accaggaatg gcccaccgcc ctggaagtcc gccagcagct gctcaaacgc cgccccggca
      181 cccctgactc cgccgactgg cacgccctgg ccggctgtgc cctgcaagcc caggtcccgg
      241 ctgaagcgat ctcagcggct gaaaaagcac tcaagatcga cgcccaggac ggcatcgccc
      301 atgcgctgta tggggcggcg ctcctgcagc aaaacgatgc cgaaggggct ttgagccatt
      361 tcagcactgc cactgagctg gctccgcaca aagccgaacc gtggctggcc ctggcagagg
      421 cgtattctca ctccaatgat ccggtccggg cattcgatac cttgcgctcg ggcagcctgg
      481 cagcacccca ttcggctcgc ctgcagctgg cattgggtaa ggccagcctg gacgccggcg
      541 cgccatccca ggccctgggc gccttccgca agtcagccag cctgattcag gaaaataacc
      601 cggaagatca gccgatcggt tcggtgtatg gaatggcaga cctgcatcac gaaatcgctt
      661 tctacctggg cgaaaccctg cgccagttag gccatctcga cgaggccagg caggtgttca
      721 gcgcagcata ccaggaaacc aaccagcgta agcccagcga tacacgcctg gcgcatgctt
      781 atgccaaaac cttgctggga ctgaatgcga ccgccgaagc catccgcccg cttcaagacg
      841 tagtcgcagg tacccccgcc gacgcagacc cctatgtgga tctggcgcgc agtctccttg
      901 agctgggcgg tcctcgccgt gattacgccg cgcagtctat agagtacctc cagcaggcac
      961 tcgcgatcaa tcccaattat gccgaagccc agggtttgtt gggcgaagca atggaagcca
     1021 atcaccagcc aaccagggca ttaagtgcct atcagaaggc gcttgaaaca gccctgatcg
     1081 atgaccctgc ctggaaagcc cgcctgcagc acggcgtcgg tcgggctgcg ctacagaatg
     1141 gccaggctga aacagccctg gtcagcctgc gcgaagccgc ccagctcgaa ccactcaatc
     1201 cgggcatcca gcagaccctg gccagcgctt accttgagct ggggatgtat gccgacagcc
     1261 tgaacgcagc ccgcaccgcc ctgcagctca accccgcgca gacccaggta atggcctggt
     1321 ttgcccaaca cgcccgcagc ctgctgagcg cttccgatgg ccagattaat gccctgccgg
     1381 aagccggcaa agcccttgcc caggcgatcc agagcgatcc ttcccagatc gagctgtata
     1441 tcgaccttgg ccagctgcat cttgaaaaca gcaaccaggc cgctgccctg cgggcgttta
     1501 tcgaagccgc ggaccgcagc agccaggtga gcctgtcgtt ggcccaggtc ttactgctgg
     1561 cccgcgagct ccgccacctt ggcgaagccg aaaaagccgc catcttgctg acccaaagtc
     1621 tccaggagta tccaggctca gacgactaca ccccagacca ggaccggccg gagactcagc
     1681 tgtggtacga gctctcgatc actcagctgc agcttggcag tcagaccgca gccatccagg
     1741 cgctcgataa agcaatcctg tgccagccgg acaatcccac cctgctggtc aagaaagccg
     1801 acctgctggt tgaatgtgac cgcctggcgg atgcatccag ccttctccag cgcttgctga
     1861 gcgaagcacc tgaagatatc gccctgtata tcaaagccgg atttgtcctg cgtgggctgg
     1921 gagaatacac caccgccctc gcttatgcgg agcaggccat cagccttgtg gaaaccggca
     1981 cacccggctc catggccgcc agggctcact ctctggctgc tcagctgggc cgcagcctgc
     2041 tgatgcccga taaagccctg gcctggatat ccagcgtcga tcccgacacc gacgagcaga
     2101 ca
//
LOCUS       DATMXK010000309         4027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_146923, whole genome shotgun sequence.
ACCESSION   DATMXK010000309 DATMXK010000000
VERSION     DATMXK010000309.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146923"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..567)
                     /locus_tag="VN363_05675"
     CDS             complement(<1..567)
                     /locus_tag="VN363_05675"
                     /inference="COORDINATES: protein motif:HMM:NF014357.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018759 - methenyltetrahydromethanopterin
                     cyclohydrolase activity [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methenyltetrahydromethanopterin cyclohydrolase"
                     /protein_id="HWQ84034.1"
                     /translation="MISINRQAHKIVDQMVEQAEPLGIGVSYLPDGARVIDIGLQAPG
                     GLLAGLITANASLGGMGQVNFQLLDYGEFSLPGVCVAVDQTEIGCMAAQYAGWGIQRG
                     EFFAIGSGPARALSRGEAVFQQLDYADSAEVAVIILEGRQMPTDTVAEYLARKCHVPA
                     QGLTMIMAPAACLAGAVQLVGRVVATGLH"
     gene            complement(776..2329)
                     /locus_tag="VN363_05680"
     CDS             complement(776..2329)
                     /locus_tag="VN363_05680"
                     /inference="COORDINATES: protein motif:HMM:NF021476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2193 domain-containing protein"
                     /protein_id="HWQ84035.1"
                     /translation="MANKVIYKKMIAEASAAADADLGVIGKKRGGPFVFTDAKPYVDA
                     VNGMKPGEGQSKEIFDLHVQSVNAHYDIFNAIAVNNTIRPEDDPFVEHYQTGPILELL
                     YDSDPEFKKSMWKFIDAIAKPENVALIGREATARYGGMYGLTCVVDFGLSVGSVPNTV
                     NQILRPLDIPKDHKRAILAAKSWGMTTSYGLGGAFRGALQSGKSLSEAEKAEIDMLQF
                     IYREPIAAQNHLMATHNLGGHGPHTSFDTNDYQKRYQTRMKPYVEAAVSAGVHYGNIL
                     VVPAYCVGDVGHHLGPATYNMFKDDMVFNIYTAVTKVYESTLQKGLEMDAYKSEWDVL
                     SVATGSGACAVAYILWLDSFTVPMVVDLLNKRFHNYAAMNPGRGEADELHNADFIDIL
                     KRGEQILDIEPKGNGAMIKGIPVDLSQVNSNEVVMNPQRYTYPACAITQRFAALMSLS
                     DYPCFLTTECVTATLMTNIIALAPNKVPAPIRSCKDCATTDLIKRNVPYMKAYGVGPK
                     GLCQWADVV"
     gene            complement(2614..2874)
                     /locus_tag="VN363_05685"
     CDS             complement(2614..2874)
                     /locus_tag="VN363_05685"
                     /inference="COORDINATES: protein motif:HMM:NF012675.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015036 - disulfide oxidoreductase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutaredoxin family protein"
                     /protein_id="HWQ84036.1"
                     /translation="MPCKCMGSVYITIYRTPDSKEGRELKAYFDRRGVAYEDLDVSKD
                     PKELQRMQELSGQVERPVALIDEKVMIGYDPVELEPLVPSFF"
     gene            3415..3951
                     /gene="folK"
                     /locus_tag="VN363_05690"
     CDS             3415..3951
                     /gene="folK"
                     /locus_tag="VN363_05690"
                     /EC_number="2.7.6.3"
                     /inference="COORDINATES: protein motif:HMM:TIGR01498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003848 -
                     2-amino-4-hydroxy-6-hydroxymethyldihydropteridine
                     diphosphokinase activity [Evidence IEA];
                     GO_process: GO:0009396 - folic acid-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-amino-4-hydroxy-6-
                     hydroxymethyldihydropteridine diphosphokinase"
                     /protein_id="HWQ84037.1"
                     /translation="MGEEHRAFLGLGTNLGDRQSNLLQALQLIRSIAVIQKISSIYET
                     KPVGYLDQPDFLNLVCQITTGLSAIELLQALKLIETQLGRQASFRNAPRVIDVDILLF
                     DDLVMQSPDLTIPHLRLAERAFALIPLAEIAPDLVHPGLQSPIAQILTGVDVSGVKLF
                     SRELQPSPSEGGQNGPPS"
BASE COUNT          854 a         1173 c         1008 g          992 t
ORIGIN      
        1 gtgcaaaccg gttgccacta cccgccccac cagctggaca gcccctgcca ggcaggctgc
       61 cggcgccatg atcatggtta gcccttgcgc cgggacatgg catttgcgag ccaggtattc
      121 tgctaccgta tcagtgggca tctgccgccc ctcgaggatg atcaccgcta cctcggccga
      181 atcggcataa tccagttgct gaaacaccgc ctcgcctcgc gagagtgctc gcgccggtcc
      241 ggagccgatt gcaaaaaact cacctcgctg gatgccccac cctgcatatt gcgccgccat
      301 gcagccaatc tcggtctgat ccacggctac gcataccccc ggcagggaaa actcgccata
      361 atccagcagc tggaaattaa cctgtcccat gcctcccagg ctggcattgg cagtgatcag
      421 gccggccagc agcccgccgg gtgcttgcag gccgatatcg atcacccgcg ccccgtcggg
      481 caggtaagag actccaatcc ccagaggctc tgcttgctcg accatctgat ccacaatctt
      541 gtgcgcttgc cggtttatgc tgatcatatt ttccttgcct tcagccgcct tcggattgca
      601 ctctggctgt gtaccaaatc gagcgcgcag gggttggctg cgcgctcgcg aagtacaact
      661 tcacccgtgt gatcgggtaa tatcaagggt attgcgcgcc tgttcgagcc tctgggcaca
      721 tcccgatctg gatcgggatg cgcctcagtt aactcgctac ggctattctc acaaactaga
      781 cgacatctgc ccactggcac agacccttgg ggccaacgcc gtaagctttc atgtaaggca
      841 cattccgctt gatcaaatcg gtggtcgcgc agtccttgca cgagcgaatc ggggcgggta
      901 ccttgttggg ggccaacgcg ataatgttgg tcatcagggt cgccgtgacg cactcggtgg
      961 tcaggaagca cgggtaatcg gacaggctca tcaaagctgc gaagcgctgg gtgattgcgc
     1021 acgcagggta agtgtagcgc tgcgggttca tcacgacttc gttgctgttg acctggctca
     1081 ggtccacggg gatgcccttg atcatggcgc cgttgccctt gggctcgata tccaggattt
     1141 gctcgccgcg cttgaggatg tcgatgaagt ccgcattgtg cagctcgtcc gcttcgccac
     1201 ggccagggtt catcgccgca tagttgtgga agcgcttgtt cagcaggtca acaaccattg
     1261 gaacagtgaa ggaatccagc cacaggatgt aagccactgc gcaggcgccc gacccggtgg
     1321 cgacgctcaa tacgtcccac tcgctcttgt aggcatccat ctccaggccc ttctgcagtg
     1381 tgctctcata gaccttggtg accgcggtgt agatgttgaa caccatgtca tctttgaaca
     1441 tattgtaggt cgctggtcca aggtggtggc ctacgtcacc cacacagtaa gcgggtacaa
     1501 ccaggatgtt gccataatgg acgccggcgc tcaccgcggc ctcgacatac ggcttcatcc
     1561 ttgtctggta gcgtttctgg taatcgttgg tatcgaagga ggtgtgcggt ccgtgcccgc
     1621 ccaggttgtg ggtcgccatt aaatggttct gagctgcaat cggctcgcga tagatgaact
     1681 gcagcatatc gatttcggcc ttctcggcct cagacaggct cttgcccgac tgcagtgcgc
     1741 cgcggaaagc tccgcccaga ccataggagg tggtcatccc ccaggacttg gcggccagga
     1801 tggcgcgctt gtgatctttg gggatgtcca atgggcgcag aatttgattg acggtgttgg
     1861 ggaccgagcc taccgagagc ccaaagtcaa cgacgcaggt caggccgtac atgccgccat
     1921 accgcgcggt agcttctcgc ccgatcaatg ccacattttc tggcttggcg atggcatcga
     1981 tgaacttcca catcgatttc ttgaattccg gatccgaatc gtagaggagc tccaggatgg
     2041 ggccggtctg ataatgttcc acaaatggat catcttccgg gcgaatggtg ttgttcacag
     2101 caatcgcatt gaagatatca tagtgggcat tgacggactg cacgtgcaga tcgaagattt
     2161 ccttcgattg tccctcgcct ggtttcatgc cgttgaccgc atccacatac ggctttgcgt
     2221 ctgtgaatac aaaagggcca ccccgcttct tgccaatgac ccctaaatct gcatctgccg
     2281 ctgctgaagc ctcggcaatc atcttcttgt agattacttt attggccatt atattctcct
     2341 tacgtttgca ttcaatttga ctatcaggtt tggttgaact aacgcaggtc tactctcgat
     2401 ctcagaccac tttaattttt ctaatccttg cacctcctct accttgaata ctttattcct
     2461 gttcctccag ccatctatgc cttgtcatgt tcatgacctt catcggcgtt ttcgacgttg
     2521 tcgggctctc ggagtatgct gcgttgcagc ttcaagaact cctcaaacct ctgcaaattg
     2581 tgatcgtcta aaccatcctg agatcaagcc caatcagaaa aatgacggca cgagaggttc
     2641 cagctcaacc ggatcatagc cgatcatgac cttttcgtcg atcaaggcaa cgggtcgctc
     2701 gacctgtccg gagagctcct gcatccgctg cagctccttg ggatcttttg atacatccaa
     2761 atcctcgtaa gctacacccc tccgatcgaa gtaggctttc aactctcgcc cttccttcga
     2821 gtcaggagtc ctatagatcg ttatatatac ggatcccata catttacacg gcataatctt
     2881 cacctcatat cgacagcaaa ctggcagcca ttaatcagaa attctgcttg gtgtgggcga
     2941 taatttgtaa ctgatctggc ggattaacac aaaaaccagc ctctattgcg atcttcttca
     3001 caaatggatg gctggtaaca atttgtgtcc aacaaacctc ctcgaataaa cctgatcagg
     3061 agaatgagca gctctacgaa aagtttaatt gagtgtcgcc cctggcgggt aggttaagtg
     3121 ccgctcaatc gattgcttct cggcagctcg gctgttggac acagctctgc ggtcccagtt
     3181 gcccgggtag cctatttaga tgcattaaga atctacatgt taacaaaagc tttgtcaagt
     3241 gattaatgta atattcgagt gcgtgatcct ctgcagctac tcctccgcca tccaccgttc
     3301 cttgacagaa gggattagtt ggagtatctt caaaaaacct gctcaaaatt tttccctggc
     3361 ctatttctga tgcaaaataa aaccaggcct ctgatttcga aatgagtgaa attcatggga
     3421 gaagaacatc gagcctttct cggtttaggc actaatctcg gcgaccgtca gagtaatctc
     3481 ttacaggcgc tccaattaat ccgttccatt gcggtcattc agaaaatatc ctcgatttac
     3541 gaaaccaaac cggttggtta cctggaccaa cccgatttct tgaacctggt ttgccagatc
     3601 acgaccgggc tttcggcgat cgaactgctc caggctctca agctcatcga aacccagctc
     3661 gggcgccagg cctcattccg caatgcgccg cgcgtgattg atgtcgacat cctgcttttt
     3721 gatgacctgg tcatgcagtc ccctgacctg accattcccc accttcgcct tgccgaacgt
     3781 gcctttgccc tgatccctct cgccgagatt gctcccgatc tggtccatcc cggcctgcag
     3841 agtccaatcg cccagattct taccggggtt gatgtttcag gggttaaact tttttcacgt
     3901 gagctgcagc cctcccccag cgaaggaggc cagaatggac ctccttcttg agagaattcg
     3961 caagcataat gcctttgccg cccggcatgc taccctgcgt gatctccgcc agcagatcct
     4021 ccacggt
//
LOCUS       DATMXK010000310         4548 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_46841, whole genome shotgun sequence.
ACCESSION   DATMXK010000310 DATMXK010000000
VERSION     DATMXK010000310.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4548)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4548)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4548
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46841"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..1042)
                     /locus_tag="VN363_05695"
     CDS             complement(83..1042)
                     /locus_tag="VN363_05695"
                     /inference="COORDINATES: protein motif:HMM:NF013761.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="proline dehydrogenase family protein"
                     /protein_id="HWQ84038.1"
                     /translation="MRSFFIFLSKARWARNLVMHWSVAWKVASRFISGEKLEDAVRVI
                     RSLNGKGINASLDHLGEDTDAPESALRATQAILDCFDAIDAAGVRANVSLKLSQLGLG
                     ISRELCIENMRRIMTRAKASNNFLRIDMEDHTLVDATLEIFHLLRSEFGPQYVGIVIQ
                     SYLYRSEADLQQILARDGKVRLCKGAYDEPAQVAYPVKANVDANFDRLARQLMDCELA
                     AGQPGGTADGRLPPITAIASHDEVRIQNAKAYAASIGLAKNAIEFQMLYGIRRELQEK
                     LAQEGYPVRVYVPYGTEWYPYFMRRLAERPANVWFVLGNFFRK"
     gene            1275..2708
                     /locus_tag="VN363_05700"
     CDS             1275..2708
                     /locus_tag="VN363_05700"
                     /inference="COORDINATES: protein motif:HMM:NF016827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84039.1"
                     /translation="MNQSQSQVLHHGLRPLTTAHLAQTMTLLELSSVELEQKIEAELA
                     ANPALELQEQAHCPQCHRRLPPSRICPVCSRPASAVDEQPIIFVSPRTDFATYDYRGA
                     SSSHSSDDMAGEEWAAEAEDLPTYILRQIAPELDKEDRILAAHILTSLDDDGLLNVPL
                     SEIALYHRVRMARLENILSLIQHADPPGVGSPTPQQALLVQLEILEETGDVPPLAAAA
                     IQKGMDLLSRHAYMELGKRLKVSAAEAQEIARFISENLNPYPARAHWGSVRHSTHAQP
                     NYPSPDVMISRLTNEPTTPLVVEVISPYAGMLRVNPLFRQSLAQASDSTAEKWQNDLD
                     SAVLLVKCLQQRDHTLVRLMQQLVVIQRQFILKGDAFLLPVTRASLAETLSVHESTVS
                     RAVSSKSILLPNGRIIPMSRMFDRSLHVRTALRCIVAAERRPLSDTQIARLLGDQGYD
                     VARRTVAKYRNMEGILPARLRAASMKA"
     gene            2705..>4548
                     /locus_tag="VN363_05705"
     CDS             2705..>4548
                     /locus_tag="VN363_05705"
                     /inference="COORDINATES: protein motif:HMM:TIGR00229.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ84040.1"
                     /translation="MSLNSSFWTRKSRQLGQAELEGATEFIPQAVLVMDSYLRVVAVN
                     NAACELFSRPRVMLDGKFLPELIKLPEDFPQRLSSDQSPPDLILAELNLPGNACQDVN
                     LRLTGLSPGSEYLLVTLELAPLHQTSQASPETHAQFWQGMFLLASATEQAGIDQCLRT
                     ALDGARSLIPCQVAALYLCKSGETNPSEKDPPTIPLANRSAARWGDDDTLPAFVPPQD
                     LVLLRTIRVWDSSKRPPTSLHRAAREAGFTYLAVAPLGLPQKLSGLIVLGDKEKDPPA
                     ELLRLIQLIAVVMARILDDQDRRLHLDRTIDSQSIQLRVMDVAMGVVQDGIFVLSPDM
                     KLRRINQAAETIFGYKNREIEGQPVDQILLGSEQILPALHLAQSGAPTYHLNNINLLR
                     RYGDRFKALVRVQPIFHEGTVESILVIVRDLSELAAYEAHARQLAQHAELGEVMSAFA
                     HEVRNPIHSISSGLQFMDRSLAQDDPTRETVGMMLMDVDRVNELMKSMLSTTRTTDIK
                     PRPIDLGKVVTSLVERLKPTITREGISCQINVSPGLPQVNGDIRALEQIFNNLINNAI
                     QAMKGRPQSQLIVKVQSQKAAKDRLLLEVSIADNGPGIPKENLDKIFQP"
BASE COUNT         1023 a         1374 c         1225 g          926 t
ORIGIN      
        1 attttaccac tccaccaata aacctgcaag gggggagaac cccattcggt gggtgccggg
       61 atagcgatta aagaattctg gcttactttc ggaaaaaatt acccaggaca aaccagacgt
      121 ttgccggtcg ttccgccagg cggcgcataa aataagggta ccattccgtg ccatagggca
      181 catagacccg cacgggatag ccttcctggg ccagcttttc ctggagctcc cggcggatgc
      241 cgtacagcat ctggaactcg atggcgtttt ttgccagacc gatactggca gcataggcct
      301 tcgcattctg aatgcggact tcatcgtgag aggcgatcgc cgtgatcggc ggcagcctgc
      361 catcggctgt cccacccggc tgcccggctg ccagctcgca gtccattaac tggcgggcga
      421 gacggtcaaa attcgcatcg acgttcgcct taaccgggta tgccacctgg gccggctcgt
      481 cataagcccc cttacacagg cgtaccttgc cgtccctggc aaggatctgc tgcaggtccg
      541 cttcgctgcg gtacaggtaa gactggatga caattccgac gtattgcggg ccaaattcag
      601 agcgcagcag gtgaaatatc tcgagcgtgg catcgaccag ggtatggtct tccatatcga
      661 tgcgcaagaa attattggaa gcttttgccc gggtcataat gcggcgcata ttttcgatac
      721 acagctcacg gctgatcccc aggccgagct gggaaagctt caaggataca ttggcgcgca
      781 caccggcggc atcgatcgca tcgaaacaat ccaggattgc ctgcgttgct cggagggcag
      841 actcgggggc gtcggtatcc tcacccagat gatccagaga tgcattaatc cctttgccgt
      901 tcaggctgcg gataacccgg acggcatctt cgagtttttc tccggagata aagcgggaag
      961 caactttcca ggcgaccgac cagtgcatga cgagattgcg tgcccagcgg gctttagaca
     1021 gaaagatgaa aaaggaacgc atagcagacc ttcgactttg ggagttatcc acaagtagtc
     1081 ttgagctcct agataccaga gctccaagcc attattcatt gtagcataag ggtttaccca
     1141 cctcccatcc tgaaagtcat acaaggcggt tgacaatctt cacatcttta tgcgagcctg
     1201 ttcacagcgg cgaaagatta atggcatact tcttgcaacg atgtgtgata agattaggtt
     1261 agcagggtaa aagcatgaat caatcgcaaa gccaggtcct ccatcacggt ctgcgcccac
     1321 tgacgacggc acatctcgcc cagacgatga cgctgctgga attatcatcg gttgagctcg
     1381 agcagaagat cgaagccgaa ttggctgcca atcctgccct ggagctgcaa gaacaggcgc
     1441 actgcccgca gtgccaccgc aggctgcctc catccagaat ctgtcccgtc tgcagccgcc
     1501 cagccagtgc agtggacgaa caaccgatca tctttgtgtc accgcgcacg gattttgcta
     1561 catacgatta ccgcggcgcc tcgtccagtc acagcagcga tgatatggcc ggcgaggaat
     1621 gggctgccga agccgaagac ctgcccacct atatcctgcg ccagattgcc cccgagctcg
     1681 acaaagaaga ccgcatcctg gctgcgcata ttttgaccag cctggatgac gatggtctgc
     1741 tcaacgttcc cttaagcgag attgccctgt accaccgggt gcgcatggct cgcctggaga
     1801 atatcctcag tctcatccag catgccgatc cacccggagt gggttcgccc acaccccagc
     1861 aagctttgtt agttcagctg gaaatcctcg aagagaccgg cgatgtgcct ccgctggcgg
     1921 cagcagccat tcaaaaaggg atggatctgc tttcccggca tgcctacatg gagctgggca
     1981 agcgtctgaa agtctccgca gccgaagccc aggagatcgc ccgctttatc agtgaaaatt
     2041 tgaatcctta cccggcccgg gcacattggg gcagcgtgcg ccacagcacc cacgcccagc
     2101 ccaattaccc ctcccccgac gtgatgatat cccggttgac caacgaaccg acaacccccc
     2161 tggtggttga ggtgatctct ccctacgccg ggatgctgcg ggtgaacccg ctctttcgcc
     2221 aatccctggc ccaggcatcc gattcaacag ccgaaaaatg gcaaaatgac ctcgatagcg
     2281 cggtactgct ggtaaaatgt ctccagcagc gcgatcacac gctggtccgc ctgatgcagc
     2341 agctggtcgt catccaacgt caatttatcc ttaaaggtga tgcctttctg ctgcctgtaa
     2401 cccgcgccag cctggcagag accctgagtg tgcacgaatc aactgtttcc cgggcggttt
     2461 cgagcaaatc aatcctgctg cccaatgggc ggatcatacc gatgtcacgg atgtttgatc
     2521 gcagcctgca cgtccgcacc gccctgcgtt gtatcgtcgc cgctgagcgc agacccctca
     2581 gcgataccca gatcgcccgg ctgcttggcg atcagggtta tgatgttgcc cgccggaccg
     2641 tggcaaaata tcgcaacatg gaaggaatct tgccagcgcg cttgcgagct gccagcatga
     2701 aggcatgagc ctcaactcaa gcttctggac caggaagtcg cgccagctcg gtcaggccga
     2761 gcttgaaggc gctacagaat tcatccccca ggctgtgctg gtgatggatt cttatttgcg
     2821 cgtggtggct gtgaataacg ccgcctgcga gcttttcagc cgtccacgag tgatgctgga
     2881 cggaaaattt cttcccgagc tcattaaact tccggaagat ttcccccagc ggctgtccag
     2941 cgatcaatcc ccacccgatt taatcctggc ggaactcaat ttgcccggta acgcctgcca
     3001 ggatgtcaat ctgcgcctga ccggcctttc tccggggtcg gagtacctgc tggtaaccct
     3061 cgagctggct cccctgcacc agacgtccca ggccagccca gaaacccatg cccaattctg
     3121 gcagggtatg ttcctgctgg ccagtgcgac ggagcaggca ggcatcgatc agtgcctgcg
     3181 gacagccctg gatggcgccc gcagcctgat tccatgccag gttgcggcgc tctatctatg
     3241 caaatccggt gaaaccaatc ccagtgagaa agatccacca accattcccc tggcaaaccg
     3301 cagcgccgcc cgctggggag atgatgatac tctgcctgcc ttcgtccccc cccaggacct
     3361 ggtcctgctg cgcaccatcc gggtatggga cagcagcaaa cgccctccta ccagcctgca
     3421 ccgggctgcc cgcgaagctg gtttcaccta cctggccgtt gcgccgctgg gtctgcccca
     3481 gaagctaagc ggtttgattg tcctgggaga taaagaaaaa gatcctccgg ctgagctttt
     3541 acgcctgatc cagctgatcg cggtggtcat ggcaaggatt ctggatgacc aggaccggcg
     3601 cctgcatctg gaccgcacca tcgacagcca atccattcag ctgcgggtca tggatgtcgc
     3661 gatgggggtt gtgcaggacg ggatatttgt cctgtcaccg gatatgaaat tgcgccggat
     3721 caaccaggct gctgagacaa tatttggcta taaaaatcgc gagatcgaag gccagccagt
     3781 cgatcagatc ctgctgggga gcgagcagat ccttccagcg ctccacctgg cgcaatctgg
     3841 tgcgcccaca taccacctga ataatattaa cctgctgcgc cgctacggtg atcgattcaa
     3901 agccctggtg cgtgtgcagc ctatattcca cgagggtacc gttgaaagca tcctggtgat
     3961 agtgcgcgat ctcagcgagc tggcagccta cgaagcgcat gctcgccagc tggcccagca
     4021 tgccgagctg ggtgaagtga tgtccgcttt cgctcacgaa gtccgtaatc cgatccacag
     4081 catcagctcc gggctgcagt ttatggaccg ctccctggcg caagatgatc caacccgtga
     4141 aacggtcggc atgatgctca tggatgtaga tcgcgtgaat gagctgatga aatcgatgct
     4201 atcgaccacc cggaccacag acattaagcc gcgcccgatc gatctcggaa aagtggttac
     4261 cagcctggtt gagcggctga aaccgaccat cacacgggaa gggatcagtt gccagatcaa
     4321 tgtgtccccc ggcttacccc aggtcaacgg tgatatccgc gccctggaac agatcttcaa
     4381 taatctgatc aacaatgcca tccaggcaat gaaaggtcgc ccgcagagcc agctcatcgt
     4441 caaagtccaa tctcaaaagg cagccaaaga ccgcctgctg ctggaggtct caatcgccga
     4501 caacgggcca ggaatcccga aagaaaacct ggataagatc ttccagcc
//
LOCUS       DATMXK010000311         3484 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_94273, whole genome shotgun sequence.
ACCESSION   DATMXK010000311 DATMXK010000000
VERSION     DATMXK010000311.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3484)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3484)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3484
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94273"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..439)
                     /locus_tag="VN363_05710"
     CDS             complement(<1..439)
                     /locus_tag="VN363_05710"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ84041.1"
                     /translation="MPGPDFIPSFPHYLAAKKTVDDRALNGLVWQTLFDQLVGAQAES
                     PLRVLEIGAGIGTMFERLVERGMLHHAHYTALDALPENITAARSRLRPFAKQSGLRFA
                     ELPGGKLQFTGPESELTLQLVARDLFDFIEQQRGFYSWDLLIAH"
     gene            complement(448..1563)
                     /locus_tag="VN363_05715"
     CDS             complement(448..1563)
                     /locus_tag="VN363_05715"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF045194.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ84042.1"
                     /translation="MRIGLVIYGSLENLSGGYLYDRRLVAELEAHGDQIKLVTLPWRS
                     YVEHLADNASGHLHLRLQRLDVDLLLQDELNHPSLAWLNSRLKTKVAYPIVSIVHHLR
                     SSEDRPAWQNRCYALIEDRYLNSVDGFLFNSQTTRQVVESRLNQANASRIRWSVAYPG
                     GDQYSPWITGEAIRARALEPGPLRLLFVGNVIARKGLHRLLRALREIPWNQWELRVAG
                     RLDVDKRYARNIRKIVALEGLVESVRFLDHLSPAQLAAQMMECQVLVVPSTYEGFGIA
                     YLEGMGSGMTPIATTSGAAGEFITHNENGFVIPPKDKNALVNAIRRLIHDRNYLAQLS
                     LAARARYLAHPTWSESMTGAVHFLHSFASQPPSRTAP"
     gene            complement(1570..1947)
                     /locus_tag="VN363_05720"
     CDS             complement(1570..1947)
                     /locus_tag="VN363_05720"
                     /EC_number="4.1.2.50"
                     /inference="COORDINATES: protein motif:HMM:NF013411.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0070497 -
                     6-carboxy-5,6,7,8-tetrahydropterin synthase activity
                     [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-carboxytetrahydropterin synthase"
                     /protein_id="HWQ84043.1"
                     /translation="MYSVAVRRNFIAQHFLIGGDWGAENQLHSHHYQLELQLQGSQLD
                     EHGYLVDIVAIEQHLDVLVSFYRDQTLNDLPEFIGLNPSLERFAHSLCQQVNNRLRAA
                     NLHALTLTLWENESAWASYHQER"
     gene            2112..3101
                     /gene="corA"
                     /locus_tag="VN363_05725"
     CDS             2112..3101
                     /gene="corA"
                     /locus_tag="VN363_05725"
                     /inference="COORDINATES: protein motif:HMM:TIGR00383.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015087 - cobalt ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0015095 - magnesium ion transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006824 - cobalt ion transport [Evidence
                     IEA];
                     GO_process: GO:0015693 - magnesium ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium/cobalt transporter CorA"
                     /protein_id="HWQ84044.1"
                     /translation="MIRAAYYTKDDQFTTDLEPDAFKKALDDPDGLLWVDFEASRPEE
                     DEPILLDIFKFHPLAVDDALRETHVPKVDDWGQFLYIVLQDLVISETREHTLMALELD
                     VFLGENFLVTHHDQKLKAIEELWSTIQRDERHLINGPDHLLYRLVDELATGYVLLVEK
                     LDERIDSLEDEIFNHSESNILEQIFTLKRATLQFQRTISPEREVLNKLARDDYPMIDE
                     QARVYYRDVYDHMVRLHDLTESLRDLASGSLDTYLSVINNRTNDIMKTLTIITAFFMP
                     LSFITGFFGMNFFAAEPPFAAWVSLPVLVGTMVMMVGVPVGMYFWMRRQGWTR"
     gene            3114..>3484
                     /locus_tag="VN363_05730"
     CDS             3114..>3484
                     /locus_tag="VN363_05730"
                     /inference="COORDINATES: protein motif:HMM:NF015999.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YbaK/EbsC family protein"
                     /protein_id="HWQ84045.1"
                     /translation="MDQKPLSASAQKVQDALANLGMSLQVVELPGSTRTAVEAAEAIG
                     CSVGQIVKSLVFKARRSGKPILVIASGSNRVDERKIEALIGEPLGKADADFVRQQTGF
                     VIGGVPPVGHNQPLLTYLDADL"
BASE COUNT          815 a          884 c         1019 g          766 t
ORIGIN      
        1 catgggcaat gagcaagtcc cagctgtaga atccgcgctg ctgttcgata aagtcaaaca
       61 aatccctggc gaccagctgc agagtcagtt cgctttcagg gccagtgaac tgcagcttgc
      121 cgccgggcag ttccgcaaag cgcagacctg attgtttagc aaatggacgc aatctggagc
      181 gagcagcggt gatgttttca ggcagggcgt ccagggcagt atagtgagca tggtggagca
      241 tgcccctctc gaccaggcgc tcaaacatgg taccgatgcc agcgccaatt tccaggacgc
      301 gcagtggaga ttcagcctgt gcaccgacca gctggtcgaa taaggtttgc cagaccagcc
      361 cattcaaggc gcggtcatcc acggtttttt tggctgccag ataatgagga aacgatggga
      421 tgaaatcagg accgggcatc gctgagctca aggagctgtt ctggagggag gctggctggc
      481 aaagctgtgt aagaaatgca ctgcaccggt catcgattcg ctccaggtgg ggtgagccag
      541 gtaacgagcc cgcgccgcca ggcttaattg agccaaataa ttgcggtcat ggatcagcct
      601 gcggatggcg tttaccaggg cattcttatc cttgggcggg atcacgaatc cattctcatt
      661 atgggtaatg aactcaccgg ccgcgccgga ggtggtggca atcggggtca ttccagagcc
      721 catcccttcc agatacgcaa ttccaaagcc ttcgtaggtc gatgggacca ccaaaacctg
      781 gcattccatc atctgggcag ccagctgggc tggcgaaagg tggtcgagga agcgcacact
      841 ttccaccagg ccttccaggg cgacgatctt gcggatattg cgagcatagc gcttatcgac
      901 atccaggcgt ccggcaacgc gcaattccca ttggttccat gggatttcgc gtaaggcgcg
      961 gagcagcctg tgcaaacctt tgcgagcgat cacattaccc acaaaaagca aacgcagcgg
     1021 acctggctcc agagcccggg cgcggattgc ttcaccagtg atccaggggg aatactggtc
     1081 gccgccgggg tatgccaccg accagcgtat ccggctggcg tttgcctggt tcagccggct
     1141 ttcaacaacc tggcgggtgg tctggctatt aaacaagaag ccatcgacgc tgtttaaata
     1201 gcgatcttcg atcagggcat agcacctgtt ctgccaggcg gggcgatctt cgctgctgcg
     1261 caaatggtga acaatggaga cgatagggta ggcgactttt gtcttgagcc ggctgttcag
     1321 ccaggccaga gacgggtgat ttaattcatc ctgaaggagc aaatccacat ccaggcgctg
     1381 tagacgtaag tggagatggc ctgaagcatt atccgcgaga tgctccacgt aactgcgcca
     1441 tggcagggta acgagcttga tttgatcacc gtgagcttca agctcggcaa ccaatctccg
     1501 gtcgtagagg taaccaccgg agagattctc caggctgccg tagatgacca gtccaatgcg
     1561 catggttact tagcgttcct ggtggtaaga agcccaggcg gactcgttct cccacaaggt
     1621 cagggtaagg gcgtgaagat tggcagcccg gaggcgatta ttgacctgct ggcacaggct
     1681 gtgggcaaag cgctcgaggc tcgggtttaa tccaataaat tcgggtagat cattgagggt
     1741 ctggtcgcgg taaaagctga ccagcacatc caggtgctgt tcgattgcga cgatatccac
     1801 gagatagccg tgttcatcca gctggcttcc ctggagctga agctcgagct ggtaatggtg
     1861 agagtgaagc tggttctcag cgccccaatc gccgccgatc aaaaaatgtt gggcgatgaa
     1921 attccgccgg actgccacac tgtacacgct gttacctcca atcaggctga ataaatagca
     1981 ctattggtta ttctaccgca gcggctgctg gatggtgcag tatttcgatc gaattcaatc
     2041 tccccgaaat cctggcatcg gataaaattc gatacaggac cagaaccagc ttacaaacgg
     2101 tgaggtgcag gatgatccga gcggcttatt acacaaagga tgaccagttc acgactgacc
     2161 tggaaccaga cgctttcaaa aaggccctgg atgacccgga cggattattg tgggtagatt
     2221 ttgaagccag ccgacccgaa gaggatgagc ctatcttgct ggatatcttc aaatttcacc
     2281 ccctggcagt tgacgacgca ctccgagaaa cccatgtgcc taaagtggat gattgggggc
     2341 aattcctgta tatcgtgtta caggatctgg tgatcagcga gaccagggag catactttaa
     2401 tggctcttga gctggatgtc tttttagggg agaatttcct ggttacccat catgatcaga
     2461 aacttaaggc cattgaagag ctctggtcga cgatccaacg agatgaacgc catctgatca
     2521 atggaccgga tcatttactc taccggctgg ttgacgagct ggcgacaggg tatgtgctcc
     2581 tggtggagaa gctggacgag aggatcgatt ccctggagga tgaaatcttc aaccattctg
     2641 aatcgaacat cctcgagcag atattcacct tgaaacgagc gaccctgcaa tttcagcgca
     2701 cgatcagccc ggagcgggag gtgttgaata aattagctcg cgacgattac cccatgattg
     2761 acgagcaagc ccgggtgtat taccgggatg tatatgatca catggttcgc ttgcatgatc
     2821 tgactgagag tctgcgcgat ctggcgtccg gctccctgga tacatatctc tcggtgatca
     2881 acaaccgtac caatgacatc atgaagacac tgaccatcat tacggcattt tttatgccgc
     2941 tgtcctttat aaccggattt tttgggatga atttttttgc cgccgaacca ccatttgccg
     3001 cgtgggtgag tctgccggtg ttggtgggta cgatggtaat gatggttggc gtcccggtgg
     3061 ggatgtattt ctggatgcgc cgtcaaggct ggacccgtta gggagctgcc cgcatggatc
     3121 aaaaaccgct cagcgccagt gcccaaaagg tgcaggacgc cctggcaaat ctgggaatga
     3181 gcttgcaggt cgtagaattg cccggatcga cgcgcactgc cgtggaggct gcggaggcaa
     3241 tcggctgcag cgtggggcaa attgttaaat cgctggtgtt taaagccagg cgctcgggaa
     3301 agccaatcct ggtaattgcc agcggtagca accgggtaga tgagcgcaag atcgaagcat
     3361 taatcggcga gccgctgggc aaggcggatg cagattttgt gcgccagcag accgggttcg
     3421 tgatcggagg cgttccgccg gtggggcaca accagccttt attgacctat ctggatgccg
     3481 acct
//
LOCUS       DATMXK010000312         2500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_169313, whole genome shotgun sequence.
ACCESSION   DATMXK010000312 DATMXK010000000
VERSION     DATMXK010000312.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169313"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            90..245
                     /locus_tag="VN363_05735"
     CDS             90..245
                     /locus_tag="VN363_05735"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84046.1"
                     /translation="MPVGFHQTWMDVIRQPMQAGEIDVPCTSRPKWRLFGDEYNRVQV
                     FFILWNG"
     gene            complement(272..>2500)
                     /locus_tag="VN363_05740"
     CDS             complement(272..>2500)
                     /locus_tag="VN363_05740"
                     /inference="COORDINATES: protein motif:HMM:NF028476.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein ndvB"
                     /protein_id="HWQ84047.1"
                     /translation="GENRLSPWGNDPVSDEPHEAVYLRDEETAIVWSPTPQPAPGPAP
                     YLIRHGAGYSIFENHSHGLRQRLRVFASPDAPLKIIQLQLENTWDRPRRITATYYIEW
                     VLGVNRESTQQYLIPEFSSDPQALLVRNPYNTEFSGWVAFVSASNPLHGLTADRTEFL
                     GRLGSLNEPAALQRIGLAGTVEAGLDTCAALQLHVDIQPGETEQIYFVLGQAEDQDTA
                     ITLVRQFQDARQVETAWEASNQYWNDLLGAVQVHTPEPAFDLLMNRWLLYQTLACRVW
                     GRTAFYQSSGAYGFRDQLQDVMALLHVAPEEARSHLLRAARHQFEPGDVLHWWHPPSG
                     RGVRTRISDDMLWLPYVTAEYVSLTGDDTILHEKIPFRRAPVLELDQHERYGQYPESD
                     ATYTLFEHCRRALLKGMTYGKHDLPLMGGGDWNDGMNLVGIDGRGESIWLGWFLYSAL
                     QRFADLCDRLGQVEPAAGYRLRASDLAEAIERNGWDGNWYLRAFYDDGSPLGSSENQE
                     CQIDSVAQSWSVLSGAGDPQRATQAMQSVSERLVRRKQQMILLLAPPFNKTKRDPGYI
                     KGYPPGVRENGGQYTHAALWSAWAFLGIGHIEEGFELFQQINPILHADSIEKANHYRV
                     EPYVVAADIYSAAPFTGQGGWTWYTGSSSWMYRLGLEGLLGLKKKGDQIEVDPHIPSN
                     WTGYRISYRFGPGIYEFIIENPAAVNRGVQQVFLNGETLPDKLIPLSQEGGNHTVRVV
                     MG"
BASE COUNT          571 a          682 c          678 g          569 t
ORIGIN      
        1 gtattatacg aacactcatt tgactaatct taagttggct gccttcggga acgacgggat
       61 gaattctatt gggttggagt tattccaagg tgccggttgg atttcaccag acgtggatgg
      121 atgtgatccg ccagccaatg caggcaggtg aaatagatgt accctgtaca agccgtccca
      181 aatggcgtct ctttggggac gagtataacc gcgtgcaggt cttctttatc ctctggaatg
      241 ggtgaggggg tagggccggg tgtgaaaaca cttaacccat gacaacgcgc acagtgtggt
      301 tgcctccctc ttggctgagc gggatcaatt tgtcggggag ggtctcgccg tttaaaaaca
      361 cttgctgtac tccccggtta accgcagccg ggttctcgat aataaactca taaatacccg
      421 gcccaaaccg gtatgagatc cggtaaccgg tccaattgct gggaatgtgt ggatctactt
      481 cgatctgatc cccttttttc ttcagaccta ataacccttc gagacccagg cggtacatcc
      541 agctgcttga tccggtatac caggtccagc ctccctgacc ggtaaatggt gcggcgctgt
      601 agatatccgc agcgactaca tagggttcga cccggtaatg attggccttc tcaatgctat
      661 cggcatgcaa tatggggttt atctgctgaa atagctcaaa tccctcttcg atatggccta
      721 tcccgagaaa agcccaggca gaccacaagg cagcatgggt atactgacca ccgttctcgc
      781 gcacgccggg aggatatccc ttgatataac ccgggtcgcg tttggtttta ttgaagggtg
      841 gggccagcag caggatcatt tgctgcttgc ggcgtaccag acgctctgaa actgactgca
      901 tagcctgggt ggcgcgctgc ggatccccgg cgcctgaaag cacactccag gattgggcaa
      961 ccgaatcgat ctggcattcc tggttctcag aagagcctaa gggtgagcca tcatcataaa
     1021 aagcgcgcag ataccaattg ccatcccatc cattgcgttc aattgcctct gccagatcgg
     1081 aagcgcgcag gcgatagcct gcagctggct ccacctggcc caggcggtcg cacaggtcgg
     1141 caaagcgttg caaggcgctg tataaaaacc atcctagcca gatactctcg ccgcgaccat
     1201 cgatcccgac gagattcatg ccgtcattcc aatcccctcc acccatcaga ggcagatcgt
     1261 gctttccata ggtcatccct tttagcaaag cccggcggca atgctcgaat aatgtatacg
     1321 ttgcgtcgga ttccgggtac tgaccatagc gctcgtgttg gtccagctcc agcacaggcg
     1381 cccggcggaa gggaatcttc tcgtgcagga tcgtatcatc accggtaagg ctgacatact
     1441 cagccgtcac ataaggcagc cagagcatat catcgctgat ccgggtgcga actcctcgtc
     1501 cggatggtgg atgccaccaa tgtaagacat ccccgggttc gaattggtgc cgggcagccc
     1561 gcaaaagatg ggaacgagct tcttcagggg ctacgtgaag tagcgccatg acatcctgca
     1621 gctggtctcg aaaaccgtat gccccgctgg attggtagaa ggcagttcgc ccccacacgc
     1681 ggcaggccag ggtctggtat agcaaccagc gattcatcag caggtcaaaa gcaggttccg
     1741 gagtgtgaac ctgtactgcg cccagcaaat cattccaata ctggttgctg gcctcccagg
     1801 ctgtttcaac ctggcgggca tcctgaaatt gacgcaccag agtgatcgct gtgtcttgat
     1861 cctctgcctg acccagcacg aagtagattt gttccgtttc gccgggttgg atatcaacgt
     1921 ggagctgcag ggcagcgcag gtatccagcc ctgcttccac tgtcccagcc agaccaatcc
     1981 gttgaagagc tgccggctca ttcaaactgc ccagccgccc cagaaactct gtccggtctg
     2041 cagtcagtcc gtgcagagga ttgctggctg aaacaaaagc tacccaaccg gagaattcag
     2101 tattgtaggg gttacgtacc aataaagcct gaggatcgct gctaaattct gggattaaat
     2161 actgctgagt gctttctcgg tttactccta atacccattc gatataataa gtggctgtta
     2221 tgcggcgggg ccggtcccag gtattttcta attgaagctg gattattttc aagggagcat
     2281 caggtgaagc gaacacccgc agccgttgac gcaagccatg actgtggttc tcgaaaatcg
     2341 aatatccggc accgtggcgg attaaatagg gagctggccc tggagcaggt tggggagtgg
     2401 gagaccagac gatcgcggtt tcttcatctc gaagataaac cgcttcatgg ggctcatccg
     2461 agacggggtc attcccccag gggctcaacc ggttttcacc
//
LOCUS       DATMXK010000313         5598 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_151459, whole genome shotgun sequence.
ACCESSION   DATMXK010000313 DATMXK010000000
VERSION     DATMXK010000313.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5598)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5598)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5598
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151459"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..988
                     /locus_tag="VN363_05745"
     CDS             <1..988
                     /locus_tag="VN363_05745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558572.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84048.1"
                     /translation="PLILKVEDLVTRFYTPEGVVHAVNGVNFDLHEGETIGVVGESGC
                     GKSVTMHSILQLIPRPPGKIERGRALFHDSDLVKLTPEEIRHVRGAQIGMIFQDPMTS
                     LNPVITIGKQVAEPLMLHLGMNKQEAEKRVIELLELVGIPEAENRIDQYPHHFSGGMR
                     QRVMIAMALACNPQILIADEPTTALDVTIQAQIVELVKRLRDELGMAVIWITHDLGIV
                     AGLAQRVLVMYAGFIIEEASVKDLYVNPQHPYTLGLLNSLPRLDSSERHRLTTIPGLP
                     PILMQAPRGCPFAPRCPYRFERCVENPPLLDIETGHRAACWWNVQEGKPRND"
     gene            981..1958
                     /locus_tag="VN363_05750"
     CDS             981..1958
                     /locus_tag="VN363_05750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016290536.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWQ84049.1"
                     /translation="MTDKEVLLDVKNMVKHFPILAGVFQRQVGVVHAVDGITFDVRKG
                     ETFGLVGESGCGKSTAGRTILQLYRPTSGSVIFDGIDLTKTKGEDLRLLRRRMQMIFQ
                     DPYASLNPRMTVAQILAEPLVVHNAAKGKEINERIEELLDIVRLSRGFAVRYPHEFSG
                     GQRQRIGVARALALEPEFIVCDEPISALDVSIQAQVINLLEDLQERYNLAYLFIAHDL
                     SVVRHISQRVAVMYLGLLVELAARDELYSHPLHPYTQALLSAVPIPDPFKEEKRQRII
                     LEGDVPSPVNPPSGCHFRTRCPLAEKICADVEPEWREVAPNHWVACHMV"
     gene            2108..4234
                     /locus_tag="VN363_05755"
     CDS             2108..4234
                     /locus_tag="VN363_05755"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ84050.1"
                     /translation="MRNKLFLVFSLLIAASMVLSACATPTPETIIQTKEVITTVEVVT
                     EGETIVVTATPEPVVAKEFTSPDPNTLVWATFGEAETLDPALDYETAGGNILANVYES
                     LVFYNREKASEFVPALAMEVPTAENGLVTNEGKTYTFNIRPGVTFHDGADMTPADVAF
                     SYVRGVLQGSTSSPQWLFTEAYFGVGVDDVAVLVEAAETFGVEALPTLSAEQIGQLEG
                     KYYDDREAVAAVAPEVLAGVCEYAKTKVVADDAAMTVTLNLQQAWAPLISTLAGYWGA
                     VMDQDWVAANGGWDGDCATWQNYYAITSAESPFTPIANGTGPYMLDHWTQGEETVLVR
                     NDNYWRTEPIWEGGPSGPASIERIVIKKVDEWGTRFAMAQAGDADFFTVNRENVAQVD
                     PMVGEWCYYDAATDTHGACEATGDGPFRLYIGAPTVTRTDVFLNFNITNPEGGNSLIG
                     SGKLDGNGIPSDFFSDLHIRKAFNYCFDWETFISDALVGEGIQSIGVPLPGMPGYDPD
                     GPIYSYDPDKCAEEFKLADLDKDGVPAGEDPDDVWEVGFRMQAAYNTGNTTRQTIAEI
                     LASTLIAVNEKFQVEVIGLPWPTFLRNQRDQTLPVFIGGWHEDIHDPHNWYVPYLTGT
                     YGRRQSLPEDILASFQEKINAGVAETDPEARNVIYKELNQEVFDLAPTIILAVATQRH
                     YEQRWIDGWYYNPAYSDAFYYAYSKK"
     gene            4356..5420
                     /locus_tag="VN363_05760"
     CDS             4356..5420
                     /locus_tag="VN363_05760"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84051.1"
                     /translation="MTAYIIRRLLLLPILLIGVTIIIFGLLQFLTPIERSALYVRDIP
                     HTEGAIEGIIKRYGLDDPLPVQYWHWLVGSVDPITGNTVGGILRGDMGWSRVGSQPVI
                     SLIKQRFPATLELALWAVGPIIAIGLWLGVKAAVNHNKPFDQIARVFAIFASSFPVFV
                     FGLLMLMIFYANLRWFPPGRISDWANIVIRSEAFRSYTGFYTIDGLLNGRFDIFLDAL
                     RHLILPVLTLSYVNWAVFLRVTRSSMLETLRQDYVTTARAKGVAERDVINKHARPNAL
                     IPVVTLAGFQVVSLLGGVVITETVFNYPGIGSAAAQAAQQLDVVTVLGFALFNGFILI
                     IANIVVDIMYAVIDPRVRLA"
BASE COUNT         1217 a         1537 c         1561 g         1283 t
ORIGIN      
        1 cccgttgatc ttaaaagtag aagatctggt aactcgtttt tacaccccgg agggggtcgt
       61 ccatgcagtc aatggtgtca atttcgatct gcatgaaggt gaaacaatcg gagtagtcgg
      121 tgagagtggc tgcggtaaaa gcgtcaccat gcactccatc ctccagctca taccacgccc
      181 gcccgggaaa attgaacggg gccgggcgtt gtttcatgat agcgatctgg tcaagctgac
      241 gccagaagag attcgccatg tgcgcggcgc ccagattggg atgatctttc aggacccgat
      301 gacctcctta aatccggtga ttaccattgg caagcaggtc gccgagccgc tgatgcttca
      361 tctgggaatg aataagcagg aagctgaaaa acgggtgatc gagctgctgg aactggtggg
      421 tatccccgaa gccgaaaacc ggatcgacca atatccacac catttctcgg ggggtatgcg
      481 ccagcgggtg atgatcgcca tggctctggc atgcaacccg cagatcctga ttgcggacga
      541 acccactacc gccctggatg tgaccatcca ggcacaaatc gtggaattgg tcaagaggct
      601 gcgcgatgag ctgggcatgg cagtgatctg gatcacgcac gatctgggga ttgtagccgg
      661 gctggcgcag cgcgtgctgg taatgtacgc cggatttatc attgaagaag cctcggtcaa
      721 ggatttatat gtgaatccgc agcatcccta caccctgggt ttgctcaaca gcctgcctcg
      781 tctggatagc agcgaacgcc accgcctgac aactattcca ggtttgccgc caattttgat
      841 gcaagccccc agaggctgcc catttgcgcc gcgctgccct taccggtttg agcgctgtgt
      901 tgagaatccg cccttactgg atatcgaaac cggacaccgc gctgcatgct ggtggaatgt
      961 acaggaagga aagccgcgca atgactgaca aagaagtcct gctcgatgta aaaaacatgg
     1021 tgaaacattt ccccattctg gcaggggtgt tccaacgcca ggtgggtgtg gtgcatgcgg
     1081 ttgatgggat caccttcgat gtgcgtaaag gcgagacatt tggattggta ggggagtcgg
     1141 ggtgtggaaa gtctactgcc ggacgcacga tcctgcagct ttaccggcct acctccggca
     1201 gtgtcatttt tgacgggatc gacctgacca agaccaaagg tgaggacctg cgtctcttgc
     1261 gccgccgtat gcagatgatc ttccaggatc catatgccag cctgaatcca cggatgacgg
     1321 tggcgcagat cctggccgag ccgctggtag tccataacgc ggccaagggc aaagagatca
     1381 acgagcggat cgaagagctg ctggatattg tccgcctgag ccggggattt gctgtacgct
     1441 acccgcatga attttccggt gggcagcgcc agcggattgg ggtggcgcgg gcgctggcat
     1501 tggagcctga atttatcgtc tgcgacgagc cgatctcagc tctggatgtc tcgatccagg
     1561 ctcaggtcat caatcttctc gaagatttgc aagaacgtta taacctggcc tacctgttta
     1621 tcgcccatga tctttccgtg gtccggcata tcagccagcg ggtggcagtg atgtatctgg
     1681 gtctcctggt ggaactggca gcgcgtgacg agctgtacag ccatcctctt cacccctaca
     1741 cccaggcgct gttatcggcg gtgccgatcc ccgatccatt caaagaagaa aagcgccagc
     1801 ggattatcct ggaaggcgat gtgcccagtc cggtcaatcc accctcgggc tgccacttcc
     1861 gcactcgctg cccattggcg gagaagatat gcgccgatgt tgaacccgaa tggcgcgaag
     1921 ttgctcccaa tcattgggta gcctgccaca tggtttaatt tgaacgtttg attaacttcc
     1981 tggctcagcc agggctggga tgctgtgtaa cttatggaag gaggtgagga gcttcggata
     2041 aataagaatt gaccggtaca cattcagtgt ttgtcaaacc aattctaatt cttagaggag
     2101 aagaaacatg cgtaacaaat tatttttggt gttttcgctc ttgatcgctg caagcatggt
     2161 tctgagcgca tgcgccaccc caactccgga gactatcatc cagactaagg aagttatcac
     2221 gaccgtcgaa gtcgtaacag aaggcgagac aatcgtcgtg acggcgaccc cggaaccggt
     2281 tgtagccaag gaatttactt ccccggaccc gaacactctg gtctgggcga cctttggcga
     2341 agcggaaacc cttgatccgg ccctggatta cgaaaccgcc ggcggtaaca tcctggccaa
     2401 tgtgtacgaa agcctggtct tctacaatcg cgagaaggcc agcgaattcg tgcccgctct
     2461 ggcaatggaa gtcccgaccg ccgaaaatgg cctggtcacc aatgaaggca agacttacac
     2521 cttcaacatc cgcccgggcg tcacattcca cgatggcgcg gatatgaccc cggcggatgt
     2581 ggccttcagc tatgtgcgcg gcgtcctgca gggcagcacc tcctccccgc agtggctgtt
     2641 caccgaagcc tacttcggtg tcggcgttga tgatgtcgct gtgctggttg aagccgccga
     2701 gacgttcggc gttgaagcac tgccaaccct gagcgctgag cagattggtc agcttgaggg
     2761 caaatactac gatgaccgtg aggctgtggc tgcggttgct cccgaagtcc tcgctggcgt
     2821 ctgcgaatat gccaagacca aagtcgtcgc agatgacgcg gcgatgaccg tgaccctcaa
     2881 cctgcagcag gcctgggcgc cattaatctc aaccctggct ggctattggg gtgcggtcat
     2941 ggaccaggat tgggtggctg caaatggcgg ctgggatggc gattgcgcta cctggcagaa
     3001 ctactacgca atcacttctg ctgaaagccc attcaccccg atcgccaacg gcaccggtcc
     3061 ctatatgctc gatcactgga cccagggcga agaaaccgtc ctggtgcgca atgataacta
     3121 ctggcgcact gagcccatct gggaaggtgg cccgagcggt cctgcctcta tcgagcgcat
     3181 tgtgatcaag aaagttgatg agtggggcac ccgcttcgcc atggctcagg ccggcgatgc
     3241 tgatttcttc accgtcaacc gtgaaaacgt ggcccaggtc gacccgatgg tcggcgaatg
     3301 gtgctactac gacgccgcga ctgacaccca tggggcctgc gaagctaccg gcgatggccc
     3361 gttccgcctg tacattggcg ccccgaccgt cacccgcacc gatgtcttct tgaatttcaa
     3421 catcaccaac cccgaaggcg gcaactcctt gatcggcagc ggcaagctgg atggcaatgg
     3481 tatcccatcc gacttcttct cggatctcca tatccgcaag gcgtttaact actgcttcga
     3541 ctgggaaacc tttatcagtg acgctctggt cggcgaaggt atccaatcca tcggtgtccc
     3601 gctgcccgga atgcccggct acgatccgga tggcccgatc tacagctacg atccagacaa
     3661 atgcgccgag gaattcaagc tggctgacct ggacaaagat ggcgtccccg ctggcgaaga
     3721 cccggacgat gtctgggaag ttggcttccg catgcaggca gcctacaaca ccggcaacac
     3781 cacccgccag acaattgccg agatcctggc ctcaactttg atcgcggtca atgagaagtt
     3841 ccaggtcgaa gtcatcggtc tgccctggcc gaccttcctg cgcaaccagc gcgatcaaac
     3901 cctgccggtc ttcatcggcg gctggcacga agatatccac gacccgcaca actggtacgt
     3961 cccctacctg accggtactt acggacgccg ccagagcctg cccgaagata tcctcgccag
     4021 cttccaggag aagatcaacg ccggcgtggc tgagactgat cccgaagccc gcaacgtgat
     4081 ctataaagaa ttaaaccagg aagtcttcga cctcgctcca accattatcc tggccgtcgc
     4141 cactcagcgc cattacgaac agcgctggat cgatggctgg tattacaacc cggcgtactc
     4201 tgacgccttc tactacgcct actcgaagaa gtaatttcgt tcaagttgtg gtgggggctg
     4261 ggtaaccagc ccccactctt cccctaaaaa tgcgtttttc tgttgcttac ctttggttcg
     4321 tgatttttta aagaaggttg gtgaaaggta aataaatgac cgcttacata atccgccgtt
     4381 tgctgctctt gccaattctg ttgattggag tcaccataat tatctttgga ttgctccagt
     4441 tcctgacccc aattgaacgt tctgctttat acgtgcggga tatcccgcat accgaaggcg
     4501 cgattgaagg gattatcaag cgctacggat tggatgatcc tctgccggtc caatactggc
     4561 actggctggt ggggagtgtc gatccgatca ctggcaatac agtcggcggt attttacgcg
     4621 gcgatatggg atggtcccgg gtagggtccc agccggtaat cagcctgatc aagcagcgct
     4681 tcccggcaac attagagctg gcgctgtggg cagtaggtcc gattattgcg attggcctct
     4741 ggctgggagt taaagctgcg gttaatcata ataagccttt tgaccagatc gcccgtgttt
     4801 ttgcaatttt tgcttcttct tttcctgtat ttgtgtttgg cctgttgatg ctgatgattt
     4861 tctacgccaa tctccgctgg ttcccgccag ggagaatctc tgattgggcg aatattgtta
     4921 tccgttctga ggcgtttcgg agttataccg gattctatac catcgacggg ttattaaatg
     4981 ggcgttttga tatctttctg gacgccttgc gccatctaat tctgccagtt ctgacacttt
     5041 cttatgtgaa ctgggcagtt ttcttgcgtg taactcgttc ctcgatgctg gaaaccttgc
     5101 gccaggatta tgtaaccact gcgcgggcca agggggtggc agagcgggat gtgatcaaca
     5161 aacacgcgcg acccaacgcg ctgataccgg tagttaccct ggctggtttc caggtggtca
     5221 gcttgctggg cggggtggtg atcaccgaga cggtctttaa ttaccccgga attggctccg
     5281 cggcggcgca agctgcccag cagctggatg tggtaactgt tctgggcttt gccttattca
     5341 acggctttat tctgattata gccaatattg tggtggatat catgtatgct gtaatcgatc
     5401 cccgggtgcg cctggcataa ccgccggtag attaatggaa aggaaacaag atgactacca
     5461 caactaaacc agacgtaccc tctttagagc atgagctgct ccctgagacg ctttcgaccc
     5521 gtaagattgg ccgcgtggaa aagctggttg gaccagacgc ttaccgggta ctgcacggta
     5581 tggtcaccac acctactt
//
LOCUS       DATMXK010000314         4474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_150425, whole genome shotgun sequence.
ACCESSION   DATMXK010000314 DATMXK010000000
VERSION     DATMXK010000314.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4474
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150425"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            122..1396
                     /locus_tag="VN363_05765"
     CDS             122..1396
                     /locus_tag="VN363_05765"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWQ84052.1"
                     /translation="MAASAIPGPENITRQTLGNGITVLARPNFNSPSITITGYLQVGG
                     LVDPDEKLGLALFTAACLTRGTSRRSFQEFHEALEAAGARLGIASGTHTTSFHGQALA
                     EDLDLLLGSLAEALTQPNFPIEQIERLRTQLLTGLAMRAQDTEEMAGLICGRLLFGSH
                     PYSRPGDGYPETIQAITRQDLSDFHARAYGPRGMIIVVVGGIDPSLAIERVNAALGGW
                     QNPVQPPPAILPDLIPLNLPVRQDYFIPGKSQADLIAAWIGPTRHSPDYLPAALGNNI
                     LGEFGMMGRIGEVVREKAGLAYSAQSSFSAGIGPGTWSVDAGVDPVNVNQALDLIQSE
                     VRRFTSEPVSLSELVDSQDNYIGSLPLSLESNIGVASALMTLEKYQLGLDYYLTYADR
                     IRAVTVEQVLETAQRYLDPECMAVAVAGTLVK"
     gene            1414..1788
                     /gene="acpS"
                     /locus_tag="VN363_05770"
     CDS             1414..1788
                     /gene="acpS"
                     /locus_tag="VN363_05770"
                     /EC_number="2.7.8.7"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560896.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008897 - holo-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="holo-ACP synthase"
                     /protein_id="HWQ84053.1"
                     /translation="MLHISSGIDLIEIDRVIRAIDRHGDRFLTRVFTPQELAELQGNP
                     ASLAGRFAAKEAVAKALGTGIGVVAWQEIEILRGPAREPVLHLHGAAAELAAQKNLTS
                     WSVSLSHAQTLAVAAVFAVGQT"
     gene            1932..4445
                     /locus_tag="VN363_05775"
     CDS             1932..4445
                     /locus_tag="VN363_05775"
                     /EC_number="3.5.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013927.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016811 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds, in linear amides
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin acylase family protein"
                     /protein_id="HWQ84054.1"
                     /translation="MAASKIGRGLVILLSVVLILTLIGSVLGVITVRRSFPVVDGNLQ
                     VPGLIAAVDIYRDTSGIPQIYAANQHDLFFAQGFVHAQDRFWQMDFWRHLSAGRLAEM
                     FGQDQVETDSFLRTLGWRRVAQQELEAVDADTRSILEAYAAGVNAYLVDHQGSAVSLE
                     YAILALLNPDYAPKPWQALDTVTWAKVMAWDLGANMDDEIEKAILSKSFTAEQLAQIY
                     PNYPEDGPVIVKDFPGTSGAVDEDQTRAGVAGAIQPALDEIASRLANIPPVLGERGIG
                     LGSNSWAVSGPRSATGMPILANDPHLGVQMPPIWYQIGLHCLPVSSECPIEVAGFSFA
                     GVPGVVIGHTARIAWGFTNTGPDVQDLFVEKINPENPEQYEVNGVWQDMVLVNESIRV
                     AGGEEVPLTVRYTRNGPIISETYALLEEFSNRSGIDLPQTYAISLRWTALEAGNTISA
                     IMQFSKAQNWDEFRAASRQFVAPSQNLLYADVDGNIGYQMPGNIPIRVHGDGTLPVPG
                     WTDEYQWQGYIPFEELPYVFNPPEGYIVTANNAVVGEEYPYSITTAWDYGYRASRIIE
                     LIENASGPLSLEDMQQFQADNKDLNAELLVPILLSIPMDDSRLAAGQALLEGWDFQDQ
                     MESASAGLFAAFWKHLLARTFVDELPEDYQPEGSSRWFAVMHRLVAQPDSTWWDDQST
                     QTIERRDDIFRLALEGAIDELEGILGKDPQDWKWGRLHTVLFENASLGESGIAPIEAL
                     FNRGPFETSGGSSIVNATGWKANKGYEVTSLPSMRMLIDLGNLDNSLTIMTTGESGHA
                     YHPNYIDMADDWRMIQYRAMLWSRAQVEDNAPNQLHLQP"
BASE COUNT         1025 a         1099 c         1274 g         1076 t
ORIGIN      
        1 gagtatattt gccaacagaa ccacctgcca gtagctttga agctgacctc aatctggaaa
       61 tggaattaga acaatgattg aatcacagtc gcctgcccga agaggagcag caaaatcatc
      121 catggcagcc tcagcgatcc ctggaccaga gaatattact cgccagaccc tggggaatgg
      181 gattaccgtt ctcgccagac caaattttaa cagcccctcg ataacaatca caggctatct
      241 acaggtgggg gggttggtcg atccagatga aaagctgggt ctggcactat ttacggcagc
      301 ttgcctgacg cgcggtacat cccggcgcag ttttcaagaa tttcatgaag ctctcgaagc
      361 ggctggagca cggctgggga tcgccagcgg cacacatacg accagttttc atgggcaggc
      421 actggctgag gatctggatc ttttgcttgg ctcgctggca gaggcgctga ctcaacccaa
      481 ttttcctatt gaacaaattg aacgtctgcg tacccagctc ctgacaggcc tggcgatgcg
      541 tgctcaggac acggaagaga tggctggatt gatctgcggc cgtttgttgt ttgggtccca
      601 tccttacagc cggccgggag acggctaccc tgaaacgatt caggcaatca cccgccagga
      661 tctgagcgat tttcatgccc gcgcttatgg acccaggggt atgatcatcg tggtcgtggg
      721 aggtattgat ccatcccttg ctatcgagcg cgtaaatgct gctctgggtg gatggcagaa
      781 tccagtccaa cctccacccg cgattctgcc tgatctgatc cccttgaacc tgccagttcg
      841 ccaggactat tttattcctg gcaaatctca agctgatctg atcgcagcct ggattggacc
      901 aacccgccat tcaccagact atctcccagc cgcattaggg aataatatcc tcggcgagtt
      961 tggaatgatg ggcaggattg gtgaagttgt ccgtgagaaa gcggggctgg cttattcagc
     1021 ccaaagctcg ttctcggcgg ggatcggtcc cggcacctgg agtgttgatg ctggtgttga
     1081 tccagtaaat gtgaatcagg cgcttgattt gatccagagc gaagtgcgcc gtttcaccag
     1141 cgagccggtc agcctttcag agctggttga cagccaggat aattacattg gaagcctgcc
     1201 attatcgctg gaatcgaata tcggggtggc ttctgccttg atgacgctcg aaaaatacca
     1261 gcttggactt gattattacc tgacttacgc cgaccgtatc cgcgctgtca ctgtggagca
     1321 ggtgctggag acggcccagc gctacctgga tcctgagtgt atggcagtcg ccgtagctgg
     1381 caccctggtg aagtaaagcg tgagggttaa ttaatgcttc acatcagctc aggtattgat
     1441 ctgatcgaga ttgatcgtgt tattcgagcg atcgaccgcc acggagatcg ctttctaacg
     1501 cgagtcttta cacctcagga gctggctgaa ttacagggta acccggcttc tctggcggga
     1561 cggttcgctg cgaaagaagc tgtcgcgaaa gcgttaggga ccggaatcgg tgtcgtggcc
     1621 tggcaggaaa ttgagatctt gcgcggcccg gcccgcgagc cggtcctgca cttacacggt
     1681 gcagccgccg agctggctgc acagaaaaac ctgaccagct ggagtgtcag tctcagccat
     1741 gcccaaaccc tggctgtcgc ggcggttttt gctgttggac agacataatt tccctcccaa
     1801 gacatcgtta agccggcgtg atataatcct ggtattcaag acaattccga tcagctaaca
     1861 caaaaaattg caatcctgac cgaaactaat tgcggaattg ttccgtttct ataatgccgg
     1921 gagggatgaa gatggctgct tccaagatag gtcgtgggct ggtaattcta ttaagtgttg
     1981 ttttaattct cactttgatc ggatctgtgt tgggggtaat tacggtccgg cgctcatttc
     2041 cggtggtcga tggcaatttg caggttcccg gtttgattgc tgcagtggat atctatcggg
     2101 atacatctgg tattccgcag atttatgctg ccaaccagca tgatttgttc tttgctcagg
     2161 gctttgtgca cgcccaggac cgtttctggc aaatggattt ctggcgtcac ctgagcgccg
     2221 gaaggctggc agagatgttt ggccaggacc aggttgagac agatagtttc ctgcgaaccc
     2281 ttggctggcg gcgtgtggcg cagcaggagc tggaagctgt cgatgcagat acgcgttcaa
     2341 ttctggaggc atatgccgcg ggtgtgaacg cgtacctggt ggaccaccag ggcagtgccg
     2401 taagtctgga gtatgcgatc ctggcgctgc ttaacccgga ttatgctcct aagccctggc
     2461 aggcactgga tacggtgacc tgggcgaagg tgatggcctg ggatttaggg gctaatatgg
     2521 atgatgagat tgaaaaagcg atcttatcta aaagctttac agccgaacaa ttagctcaga
     2581 tatatccaaa ttatccggaa gatggccccg tgattgttaa ggactttccg ggtaccagcg
     2641 gagcagtgga tgaagaccaa accagggccg gggtggctgg cgccatccag ccagcgctcg
     2701 atgaaatcgc ttcacgtctg gctaatattc ctcctgtgct gggagaacgc gggatcgggc
     2761 tcggctcgaa cagctgggcg gtttccgggc cgcgcagcgc cacgggtatg ccgattctgg
     2821 ccaatgaccc tcatctgggt gtgcaaatgc caccgatctg gtaccagatt ggtttgcact
     2881 gtctgcccgt aagttctgaa tgcccgattg aagtggctgg tttttccttt gctggggtac
     2941 cgggtgtcgt catcgggcat actgcgcgca tcgcctgggg ctttacgaat acaggaccgg
     3001 atgttcagga cctgtttgtt gaaaagatca atccggaaaa tcccgaacag tatgaagtga
     3061 atggcgtctg gcaggatatg gtcctggtga atgaaagcat ccgggtcgcc ggcggggaag
     3121 aagtacccct gacagtgcgg tatacccgca atggaccgat catctcggag acttatgctc
     3181 tgttggagga attcagcaat cgatctggga tcgatctgcc acaaacttac gctatctcct
     3241 tgcgttggac ggcattagag gccgggaata cgatttcagc aataatgcaa ttcagcaagg
     3301 cgcaaaattg ggatgagttc cgagcagcct cccgccagtt cgttgcccca tcccaaaact
     3361 tgctttatgc agatgtagat ggcaatattg gttatcaaat gcccggaaat attccgatcc
     3421 gggtccatgg tgacgggacc cttccggtac cgggctggac ggatgaatat caatggcagg
     3481 gttatattcc ttttgaggaa ttaccctatg tatttaatcc acctgaggga tatattgtca
     3541 ctgctaacaa tgcagtggtt ggtgaagagt atccttactc gatcactact gcctgggatt
     3601 atggttatcg cgctagccgg atcatcgagc ttatcgaaaa cgcctccgga ccgctgagcc
     3661 tggaggatat gcagcaattc caggcggata acaaggacct caatgcagaa ttattagtcc
     3721 ctatcctgct ctctattccc atggatgaca gccggctcgc agcagggcag gctttgctgg
     3781 agggctggga ttttcaagac cagatggagt cagcctcggc cggattgttc gctgcctttt
     3841 ggaagcacct gctggccaga acatttgtgg atgagttacc agaggattac cagccagaag
     3901 gcagcagtcg ctggtttgca gtaatgcacc ggctggtagc ccagccagat tcaacctggt
     3961 gggatgatca atctactcag acgattgaaa ggcgcgatga tatcttccgg cttgcgctgg
     4021 aaggcgcgat cgatgaattg gaaggaattc tagggaaaga tccacaagac tggaaatggg
     4081 gccggctgca tacggtgttg ttcgagaatg catcgctggg ggaatcggga attgcgccaa
     4141 tcgaggcatt gttcaatcgc gggcctttcg agacttcagg cgggagctcc attgtgaatg
     4201 ctactggctg gaaggctaat aaaggttatg aggtcacatc tttaccatcg atgcgcatgc
     4261 tcatcgatct gggcaatctg gataattcgc tcacaattat gacgaccggg gaatccgggc
     4321 atgcttatca tccgaattac atcgacatgg ctgatgattg gcggatgatc caataccgtg
     4381 ccatgctctg gtcgcgagcc caggtggaag ataacgcccc aaaccagtta catctccagc
     4441 cctgatctga ttatacaagg attaaaaaag agag
//
LOCUS       DATMXK010000315         3125 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_46532, whole genome shotgun sequence.
ACCESSION   DATMXK010000315 DATMXK010000000
VERSION     DATMXK010000315.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3125)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3125)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3125
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46532"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..902
                     /locus_tag="VN363_05780"
     CDS             <1..902
                     /locus_tag="VN363_05780"
                     /inference="COORDINATES: protein motif:HMM:NF027964.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="saccharopine dehydrogenase C-terminal
                     domain-containing protein"
                     /protein_id="HWQ84055.1"
                     /translation="NLEITRAAIETQTSLCDLGGNTDVVLTQLALDEEARQAGISLVP
                     DCGMGPGLVNTLGAYAIELLDEAREVFIYDAGLPQTPAAPWNYELTFHINGLTNEMDG
                     EAIFIRQGRIARVPTLSEPEMIEFPGLGALEADVTSGGTSTAPWTYEGRLQTYENKVL
                     RYPGHFEWLRAFKTLGLFSEQPIIVNGETVIPRQVYHALLEPKLYSPRPRDIGIMRAI
                     ARGTKAHRPAQVQVDLWDRSEETTGFTAMERLTGFHCAIMMGFQARRDVAPGAQAVET
                     AVTASRFMAEIARRDIHFEVHWD"
     gene            complement(905..2179)
                     /locus_tag="VN363_05785"
     CDS             complement(905..2179)
                     /locus_tag="VN363_05785"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWQ84056.1"
                     /translation="MNFAQRVVLLLFAACLLAGILTGQQLYYRLTYLWGLLFFFSWLM
                     SRMALRGLEIVRRPRSLRSQVGQVYEERFELRNPGRLPRLWIEIRDESPIPASAGSHV
                     LTLVGGRESRTYMARTRLSERGVFALGPTVLASGDLFGLFPTSRRFESQDNLLVYPML
                     FNVSHFPSPAGLLPGGEALRRRTAQITSNASGVREYAPGDPLNRIHWLSTARRNRLMV
                     KEFELDPLAEVWIFVDGFRVAHTSLPRPVEEVDPRDMWRKRAPFKLPPATIEYAVSIA
                     GSLARYYLQLGRAVGLVSAGTTIHILPSDRGGRQLGKILESLAMLQADGPLPLPGQVE
                     AQARYLPRGSTVVLITPSTSDSTVKAADLLLRRGLRPVAVLLDPSTFGGYFSSAAILN
                     SLQFLGIPVVQVCNGDDLGAVLSEASRAQKLH"
     gene            complement(2183..>3125)
                     /locus_tag="VN363_05790"
     CDS             complement(2183..>3125)
                     /locus_tag="VN363_05790"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWQ84057.1"
                     /translation="IEQVIIGKRATVELAVISLLCQGHLLIEDVPGVGKTMLARSLAR
                     SLGSHFSRIQFTPDMLPSDVTGVSIFNQVTREFEFRPGPVMAQIVLADEINRATPKTQ
                     AALLEAMEERQVTVDGVTHILPRPFMVLATQNPIEYEGTFPLPEAQVDRFLMRVRLGY
                     PNTEDEIRVLDRQQIRHPFETLEQVVEVEELLAVQEQVRQIYVSPALKRYIVDLVNQT
                     RQHGEVYLGASPRGSLALYRSAQARAAMDGREYVLPDDLKALAVPTLAHRVILGPGAR
                     LRDLTAEQVVDEILNSVPVPGGDPLGKAAVEPVASQA"
BASE COUNT          622 a          965 c          988 g          550 t
ORIGIN      
        1 tcaatctgga gatcacccgg gctgccatcg aaacccagac cagcctgtgc gatctgggcg
       61 ggaatacaga tgtggtgctc acccagctgg ccctggacga agaagcccgc caggcaggga
      121 tcagcctggt tccggactgc gggatgggtc ccgggctggt caacaccctg ggagcttacg
      181 cgatcgagct ccttgatgaa gctcgcgaag tttttatcta cgatgcgggt ctgccgcaaa
      241 ctcctgctgc cccatggaat tatgagctga ctttccatat aaatggcttg accaacgaga
      301 tggatgggga agcgatcttt atccgtcagg gcaggatcgc gcgggtgccc accctcagcg
      361 agccggaaat gatcgaattt cccgggctgg gcgccctgga ggcagatgtc acctcaggcg
      421 gcacttccac cgctccctgg acctatgaag gcaggctgca gacttatgag aataaagtcc
      481 tgcgctatcc cgggcatttc gagtggctgc gagccttcaa gaccctgggt ttattcagcg
      541 aacagcccat cattgtaaat ggagagacgg ttattccccg ccaggtgtac cacgccctgc
      601 tggaaccgaa gctgtattca ccccggccgc gcgatatcgg gatcatgcgg gccatcgcca
      661 gaggcaccaa agctcaccgc ccggcgcagg tgcaggttga tttatgggac cggtccgagg
      721 agacgaccgg ctttaccgcc atggagcgcc tgaccggttt tcactgcgcg atcatgatgg
      781 gttttcaagc ccggcgtgac gtggcacccg gcgctcaggc tgtagaaacc gccgttacgg
      841 cgagccgctt tatggcagaa atcgcccggc gggatattca cttcgaagtc cattgggatt
      901 agattcagtg gagtttctga gcccggctgg cctcggacag caccgctccc aggtcgtctc
      961 cattacagac ctgcacgacc gggataccca ggaactgcag tgagttgagg atggcagcgc
     1021 tcgaaaaata acccccaaaa gttgatggat cgagcagcac cgccaccggg cgcaggccgc
     1081 gccgcagcag caagtcggca gctttgaccg tgctgtctga ggtgctgggt gtgatcagga
     1141 ccacggtgct gccgcgcggc aggtaacgcg cctgagcttc cacctgcccg ggtaagggca
     1201 gaggcccgtc cgcctgcagc atggcgaggg attccaggat cttgccgagc tggcgcccgc
     1261 cgcgatcgga gggcaggata tggatggtag tcccggcgct caccaggccc accgcccgac
     1321 cgagctgcag gtaatagcgc gccagcgagc cagctatgct gacagcatat tcgatggtcg
     1381 caggcggcag cttgaaaggc gcccgcttgc gccacatgtc gcgtgggtcg acctcctcca
     1441 ccgggcgcgg cagggaggtg tgcgccaccc gaaaaccatc cacaaagatc cacacttccg
     1501 ccaggggatc cagctcgaat tccttgacca tcaagcggtt ccgccgggca gtactcagcc
     1561 agtgaatgcg gttgagcggg tcgcccggtg catactcgcg cacgccggag gcattggagg
     1621 tgatctgggc ggtgcgccgg cgcaaggctt cgcctcccgg cagcagtccg gccgggctgg
     1681 ggaaatgact cacattgaac agcatggggt aaaccagcag gttatcctgg ctttcgaagc
     1741 ggcgcgaggt gggaaacagg ccaaacaaat cacccgaagc cagcactgtg gggccgagcg
     1801 cgaatacacc ccgctcgctc aggcgggtgc gggccatgta ggtgcggctc tcgcggcctc
     1861 ccaccagggt aagcacatgc gagccggccg aagccgggat aggggattcg tcgcgaattt
     1921 caatccacag ccgcggcagg cggcccgggt tgcgcagctc aaagcgctct tcatacacct
     1981 ggcccacctg agagcgcagg ctgcgtgggc ggcggacgat ctccaggccg cgcaaggcca
     2041 tgcgcgacat cagccagctg aagaagaaca acagtcccca gaggtaggtt aggcggtagt
     2101 agagctgctg cccggtcagg atacctgcca gcaagcaggc cgcgaacagg agcagcacca
     2161 cacgctgggc aaaattcatc gctcaggcct gactggctac tggctcaacc gcggctttgc
     2221 ccaacgggtc gccccccggg acgggcacgc tgtttaagat ctcatctacc acctgctcgg
     2281 cggtcaggtc acgcaggcgg gcgcccgggc ccaggatcac gcggtgggcc agggtgggca
     2341 cagccagcgc cttcaggtcg tcgggcagca cgtattcacg cccgtccatg gcggcgcgtg
     2401 cctgggcgct gcggtacaac gccaggctgc cgcgcgggct ggctcctaaa tagacctcgc
     2461 cgtgctggcg ggtttgatta accagatcaa caatgtagcg cttcaaagct ggagagacat
     2521 agatctgcct gacctgctcc tggacagcga gcagctcctc cacctcgaca acctgctcca
     2581 gggtttcgaa tggatggcga atctgctggc ggtcgaggac gcgaatctcg tcctccgtat
     2641 tcgggtaccc caggcggaca cgcatcaaaa agcggtcgac ctgcgcctca ggcagcggaa
     2701 atgtcccttc gtattcaatc gggttctggg ttgccaggac catgaacggg cggggcagga
     2761 tatgggtcac cccgtccacg gtcacctggc gctcttccat ggcttccagc aatgcagcct
     2821 gggtcttggg ggtggcgcgg ttgatctcgt cggcgagcac aatttgggcc atcaccgggc
     2881 cggggcgaaa ttcgaattcc cgcgtcacct ggttgaaaat ggataccccg gtcacatcgc
     2941 tgggcagcat atccggagtg aactgaatgc ggctgaaatg gctgcctaac gagcgcgcca
     3001 ggctgcgcgc cagcatggtc ttccctacgc cgggcacatc ttcaatcagc aaatgcccct
     3061 gacagagcag gctgatcact gccagctcca cggtggcccg tttgcctatg atcacttgct
     3121 cgatg
//
LOCUS       DATMXK010000316         5449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_141152, whole genome shotgun sequence.
ACCESSION   DATMXK010000316 DATMXK010000000
VERSION     DATMXK010000316.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141152"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            137..379
                     /locus_tag="VN363_05795"
     CDS             137..379
                     /locus_tag="VN363_05795"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84058.1"
                     /translation="MSYTLILHVMNEEPIVGEVENLPDKADNLVMVRNPRRLDGKDLP
                     YLADNVTSVYWPVSRLNFIEIISGREEEEIIGFVRE"
     gene            383..1141
                     /locus_tag="VN363_05800"
     CDS             383..1141
                     /locus_tag="VN363_05800"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559921.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ84059.1"
                     /translation="MSENRLRDRRILVVDDEERMVRFIRLNLEHDGFRVTEAFTGNQA
                     INSVRSALPDLVLLDVMMPDMDGFEVLRLIRETSNVPVIMLTAKGEEDDRVRGLELGA
                     DDYITKPFSPRELVSRARAVLRRTESVSGTTHGIIEVDEHLKLDFDRREVWLDGELVQ
                     LRPTEYRLLFHLVQNAGWVVTHDQLLAKVWGYEYRDEPHYVRLYINYLRKKLEVDPAN
                     PKYILTERGVGYRFVDFRRQKPGETIPLGGSPQE"
     gene            1160..1816
                     /locus_tag="VN363_05805"
     CDS             1160..1816
                     /locus_tag="VN363_05805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559840.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ84060.1"
                     /translation="MSTKQRILLVDDHEVVRLGLKALLERHQNFEVVAEAGTAREAVE
                     KVAALNPDVVVMDIRLPGGSGIEACEEIAEKYPDSKVIMLTSYAEDEMLFSAIRAGAA
                     GYVLKQIGGEDLVRAIEAVGRGEALLDPAVTQRIFQEVRKAAKEEEASAFSALTQQEK
                     HVLLLVSEGKTNREIAKALFLGEGTVRNYVSSILSKLGVSNRAEAAAYAVEHNLRDYL
                     "
     gene            complement(2259..2720)
                     /locus_tag="VN363_05810"
     CDS             complement(2259..2720)
                     /locus_tag="VN363_05810"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84061.1"
                     /translation="MTNLWVIASAIFLAVALMGIIAFILWYSRPVAETPGSVTAVLNV
                     LPAPTSTPLPATPTSPPPATSTLPAPPEGVIALGGTVQISGTGGDGLRLRQAPGLEQP
                     MRLLGAEDEIFTVTDGPQESDGYVWWYIEGLYDATRSGWAVANYLEGNQGP"
     gene            2970..4082
                     /locus_tag="VN363_05815"
     CDS             2970..4082
                     /locus_tag="VN363_05815"
                     /inference="COORDINATES: protein motif:HMM:NF012298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004672 - protein kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006468 - protein phosphorylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein kinase"
                     /protein_id="HWQ84062.1"
                     /translation="MSTQPSQPIILSNRYQLDERIGSGGMAMVFRATDRMLERQVAIK
                     LLRTDYSQDDSFRERFRQEAKAAANLAHPNIVTVHDFGLDAGRLYIIMEYVPGVDLKA
                     LIQQKGRFSVEEALPLLIQACAGIGYAHRSGLVHCDIKPQNMLVTPDGRLKVVDFGIA
                     LALASISPDDKTDVVWGSPQYFSPEQAGGSAPSPASDVYSLGVVMYEMLTGRPPFSAP
                     TTEELARAHRDDPVPSARKYNPSIPLQLEQIILKVLAKEPSQRYRTADQLGRVLVTLA
                     EQSETATSTHTPLVVDEDPSESLAPAPAPPRQQVIPAPQPPVIASAPIAASRFPARPG
                     TGTDWLTWILALLALIAVGGLIPFCLWVYYALNPPL"
     gene            complement(4196..4429)
                     /locus_tag="VN363_05820"
     CDS             complement(4196..4429)
                     /locus_tag="VN363_05820"
                     /inference="COORDINATES: protein motif:HMM:NF013026.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="bL28 family ribosomal protein"
                     /protein_id="HWQ84063.1"
                     /translation="MPPASEPALIVNERIFKMAKCAQCGKTTTFGRNRPWSKKSTPRT
                     YRPNLQPITVYMDGRKVRKVLCAKCIRTLVKTS"
     gene            4550..4906
                     /locus_tag="VN363_05825"
     CDS             4550..4906
                     /locus_tag="VN363_05825"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HWQ84064.1"
                     /translation="MTDESTPIGSIHVSQKAVARIAYHAALQSYGVVGLAAKNIADGI
                     AQVLVHDPTHGVEVSYDGQAINIDLFIVIEYGTRIKSVAHSISNAVRFNVEKSLGMPV
                     NEINVHVNGLRVSDTD"
     gene            4981..>5449
                     /locus_tag="VN363_05830"
     CDS             4981..>5449
                     /locus_tag="VN363_05830"
                     /inference="COORDINATES: protein motif:HMM:NF014754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004371 - glycerone kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006071 - glycerol metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DAK2 domain-containing protein"
                     /protein_id="HWQ84065.1"
                     /translation="MLSDDPSKRSEMLHIKRVDGQGLKKLIEAGLTWLRTNQQIVNSL
                     NVFPVPDGDTGTNMVLTMQAAFDEISESGEHNFGQMAHAVAHGALMGARGNSGVILSQ
                     LWRGFARSLDSQPFLDAPSLVGAFATARDTAYKGVVRPVEGTILTVAKDIASAA"
BASE COUNT         1375 a         1390 c         1344 g         1340 t
ORIGIN      
        1 cgtctcaacg gagagaaatt atccccaagt acgccctatc aggtagacaa cgagtctata
       61 ataaccttgg ggaagctaaa cctgaaagta ctgataaaat cctgatgtaa gctccatcaa
      121 aagagcggag gccaaattgt cttatacgct tatcctgcat gtaatgaatg aagagcctat
      181 cgtcggggaa gtcgaaaact tgcccgataa agctgataac cttgtcatgg ttcgtaatcc
      241 tcgccggctg gatggtaaag acttgccata tctggctgat aatgtcacct ctgtttactg
      301 gcctgtcagc agattgaatt tcatcgaaat tatctctgga cgggaagaag aagagatcat
      361 cggctttgtg cgagagtaga atatgagcga gaatcgttta cgtgatcgac gaatcctggt
      421 agtagacgac gaggagcgga tggtccggtt tatccgcctc aatcttgaac atgatggctt
      481 ccgggttaca gaagccttta ctggcaacca ggctatcaac agcgtgcgga gtgccctccc
      541 ggaccttgta ttattagacg tgatgatgcc tgacatggat ggatttgagg tgctgcgcct
      601 cattcgagaa accagtaatg ttccggtgat tatgcttacc gccaagggtg aagaagatga
      661 ccgtgtccgg ggactggaat taggcgccga tgattacatc acgaaaccat tcagcccgcg
      721 tgagctcgtc agtcgggctc gagcagtttt acggcggacc gagtcagtgt ctgggacaac
      781 tcacggaatt attgaagttg atgagcactt aaaactcgac tttgaccggc gtgaagtatg
      841 gcttgatgga gaactggtac aattacgacc tactgaatat cggctgctct ttcatctggt
      901 ccagaatgca ggctgggttg taacccacga ccagttactc gccaaagtgt ggggttatga
      961 gtatcgcgac gagccacact acgtacgctt atatatcaat tacctgcgaa aaaagctcga
     1021 ggtggatcct gctaatccaa aatatatcct tacagagcgt ggggtaggat atcgttttgt
     1081 agattttcga cggcaaaagc caggcgagac catcccactg gggggctcac cccaggaata
     1141 accagcaacg ggagaaataa tgtcgacgaa acaacgaatt ctgttggttg atgatcatga
     1201 agttgtacgc ttaggtttaa aagctctcct ggaacgccat caaaattttg aagtggttgc
     1261 agaagcagga acagctcgtg aggctgtgga gaaggtggct gccctcaatc cggatgttgt
     1321 agtcatggat atccgtttgc caggcggctc aggaattgaa gcctgtgagg aaattgctga
     1381 aaaatatccg gactcgaaag ttattatgct cacctcatat gcggaagatg aaatgctttt
     1441 ttcagccatc cgcgccgggg ctgctggata tgtcctgaag caaattggtg gtgaagatct
     1501 ggtacgggca atcgaggctg tagggcgagg tgaagccctg ctcgacccgg cagtaactca
     1561 gcggatattt caagaggtcc gtaaagctgc gaaggaagaa gaagcgtctg cattcagtgc
     1621 cctgacccaa caagaaaaac atgtcttatt gctggtgtct gaaggaaaaa caaatcgaga
     1681 aattgccaaa gcattattcc tgggcgaggg tacagtccga aattatgtca gcagcatcct
     1741 ttctaaatta ggtgtcagca atcgcgccga ggctgccgcc tatgcagtgg aacacaactt
     1801 acgcgattat ctctaagcca gccaattggt ttaataagaa cccttttttt gtaaatcgct
     1861 ctgtgtttat tctgccactt gttcagattg gcaaactgga catgggcata atttgcgcaa
     1921 ataatgccag ttatagatcc cataatgatg cccatcttcc cattccatag tgatcgcata
     1981 ggacccaacc gcctcaacat ttattaattt ggtcgctcgc gaatcgacaa ccggcaataa
     2041 aaatacattg gggtccggtt caggtcccat tttatcgtgc ccaccccgac attcagcgca
     2101 tggacaggca aaacgcagta atgaaaatgg ataaacactg acatgtccat cgtcccaacg
     2161 gattgtaaac tcactggaaa cacgattagc tgttatttct accggtctgg ttgataccat
     2221 tcattctcct cggacgctgt attggtatgg atttcgggtt atgggccctg attgccttct
     2281 aaataattcg ccactgccca accgctgcgc gtggcatcat aaagcccctc tatgtaccac
     2341 cagacgtatc catccgattc ttgtggtcca tcggtcactg taaaaatttc atcttctgcg
     2401 ccgagcaacc gcatgggctg ctcaagtcca ggagcctggc gcagacgcaa cccgtcccca
     2461 ccggtgccag aaatttgcac agttccccca agcgcgatca ccccttccgg cggtgctggt
     2521 agcgtagaag tcgctggggg aggactggta ggcgtagccg gcagtggtgt agatgttgga
     2581 gccgggagga catttaatac tgctgttaca gatccagggg tctcagccac cggacggcta
     2641 taccagagga tgaatgcaat aatccccatc agagccactg ccagaaaaat tgcactggcg
     2701 atcacccaca agttcgtcat tgaacgaaac cagtctgtta atctcaagca gattacccca
     2761 ggtcgaaatc gcgcctatgc gcaaagtata tcagaaccga gacaggataa attttcatct
     2821 gtccggctga taccggctag attttacttg atattccccc taaagacgaa tgatttcaca
     2881 atttagatcc tggacaggtt cgataatcta atcaccaacc atctgagcac ctgggcaatg
     2941 gaagggtgga atctggtaaa atgaggcacg tgagcacaca accttctcaa ccaatcatac
     3001 tcagtaatcg ttaccagctg gacgaacgca ttggctcggg aggcatggct atggtgttcc
     3061 gtgccaccga ccggatgctc gagcggcagg tagcgattaa gctcctccgc acagattact
     3121 cccaggatga tagtttccgc gaacgttttc gccaggaagc caaggcggca gccaacctgg
     3181 cccatcccaa tatcgtcact gtccatgatt tcgggctgga tgcaggtcgt ctgtacatca
     3241 ttatggaata tgtccctggt gtcgatctga aggctttgat ccagcaaaaa ggtcgctttt
     3301 cggttgaaga agccttgcca ttattaatcc aggcctgtgc gggcattggc tatgcccatc
     3361 gctccggcct ggtacactgc gatattaaac cgcagaatat gctggtcacc cctgatggac
     3421 ggttgaaagt tgttgatttt ggaattgctc tcgccctagc aagcatttct cctgatgata
     3481 aaacagatgt tgtttgggga tctccccaat acttctcccc ggagcaagct ggtggtagcg
     3541 caccttctcc tgcttccgat gtctactccc tgggagtggt gatgtatgag atgctcacag
     3601 gccggcctcc attttcagct cctactacag aggagcttgc ccgcgcccac cgggatgatc
     3661 ccgtgccttc tgcacgcaaa tataatcctt ccattccact ccagctcgag caaataatct
     3721 taaaggttct cgcaaaagaa ccgtctcagc gctaccgcac tgccgatcaa ttgggacgtg
     3781 tactggttac cctcgctgag caatccgaaa cagctacatc cacccatacc cccctggtcg
     3841 tcgatgagga tccctcggaa tctcttgctc cagcccctgc accaccccgc cagcaggtta
     3901 tcccagcgcc acaacccccc gtgatagcct ctgcaccaat cgctgcgtct cgatttcctg
     3961 ccaggcctgg gacaggaacc gattggctta cctggatcct ggctctcctc gctttgatcg
     4021 cggtgggtgg gttaattcca ttttgccttt gggtctatta tgcacttaac ccacctctgt
     4081 aaagaagtga cccttgtcag ttcgactacg cccgattatc atttcgagcg ctcagtgatt
     4141 aaatgaaaac cacggcttgc gccgtgatct ccggaatctg aagggttggt gtcgtctagc
     4201 tggttttcac cagcgtgcga atgcacttgg cacacaatac ctttcgaact ttccgaccat
     4261 ccatataaac ggtgatgggc tgcagatttg gacggtaagt ccgcggggta gactttttag
     4321 accagggacg gttacgacca aatgtcgtgg tttttccaca ttgtgcacac tttgccatct
     4381 tgaagatcct ttcatttaca ataagtgcag gctcacttgc cggcggcatc gcttctcttg
     4441 caagactcga tccgccctgc cagaagccgg ataagtttac cacaaagcaa aactctttac
     4501 aagcaaagtt gttatacaat caagcatctt tcacaagatc gaggagaaca tgacagacga
     4561 aagcacccca atcggcagta tccatgtgtc tcaaaaagct gttgctcgca tcgcctacca
     4621 cgcagcgctg caatcctatg gcgttgttgg cctggcagcc aaaaatatcg cggatggtat
     4681 tgcccaggtc ctggtccacg atcccaccca tggggtcgaa gtcagctacg atgggcaggc
     4741 aatcaatatc gatctgttca ttgtgattga atatgggacg cggatcaaat ccgtagccca
     4801 cagcataagc aatgccgtgc gctttaatgt cgaaaaatcc ctgggcatgc ccgttaatga
     4861 gatcaacgtc catgtcaatg ggctgcgcgt cagtgacacc gattgaaccc ttatcctggc
     4921 cgcaacactt gctgatcagg ttttaatggt ctgcatatac tcatttgagg agtccattta
     4981 atgctttcag acgatccatc aaaacgttcc gaaatgctgc acatcaaaag ggtagacggt
     5041 caaggattaa aaaaactgat tgaagctggc ctgacctggc tgcgcaccaa ccagcaaatc
     5101 gtcaactccc tcaatgtgtt cccagtcccc gatggagata ccggcaccaa tatggtgctt
     5161 accatgcagg ccgcctttga tgagatttcc gaatctggcg aacataattt tgggcagatg
     5221 gcccatgctg ttgctcacgg tgctttgatg ggggcacggg gtaactcagg agtcatcctt
     5281 tcccaattat ggcgcggttt tgcacgatcg ctggactcgc agcctttcct ggatgcgcct
     5341 tctcttgtag gcgcttttgc caccgcccgg gatacagctt ataaaggcgt cgtccggccg
     5401 gttgaaggga caattctaac ggtggcaaaa gatatagcct cggctgcag
//
LOCUS       DATMXK010000317         2326 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12238, whole genome shotgun sequence.
ACCESSION   DATMXK010000317 DATMXK010000000
VERSION     DATMXK010000317.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2326)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2326)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2326
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12238"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..473)
                     /locus_tag="VN363_05835"
     CDS             complement(<1..473)
                     /locus_tag="VN363_05835"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84066.1"
                     /translation="MAESAHHLIKAVVWDLGGVLARTVDRSPRTELARRLGMTYTGLD
                     AAVFNSPSALLASLGTISTEQHWAQVCAQLNWPLESLKEFQSLFWGGDRLDYELVDYI
                     RRLKPAYKTGLLSNNWSNLRHLIENRWQIGDAFDELVISAELGLMKPDPVIYQVAL"
     gene            complement(449..1495)
                     /locus_tag="VN363_05840"
     CDS             complement(449..1495)
                     /locus_tag="VN363_05840"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024917.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84067.1"
                     /translation="MNTVQESVITIFVPGAPDLPGLSFRGYRDETDFEKMVAVIQGCK
                     QVDQLERSDTAQDIARDYRHAPNFDITKDMLFVEMNGEVIGYYRVIWFLEDAGDAIYT
                     HFGFLLPEWRGRGIGRAVLAYLQAHIRAIAATHSPEQTMFYQSDADETERDTTALLLS
                     AGYLPARHFYTMVRPDLENIPDLVLPPGYEIRPVTDQQLPAILAASSEAFQDHWGYSS
                     ANDPTLEALMEDPDFDPGLWRVAWQGDQVAGMVLSYITRAENMEYQRLRGYTESICVR
                     RPYRKQGLAHALIAASLQALRERGMQEAALHVDTENLSGALRLYESLGFQPVERHTAY
                     RKPLEMVWPNLRII"
     gene            complement(1501..2067)
                     /locus_tag="VN363_05845"
     CDS             complement(1501..2067)
                     /locus_tag="VN363_05845"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024698.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWQ84068.1"
                     /translation="MEQDLFTGKLVRLTAVDSQRLAEADSRWGRDSEYWRLQASDPAH
                     LFSVKRSKEWLEKDLEKDATRSIFFEIHTLEDDRLIGVVGFEGFQWQHGDVFVGIGLG
                     EREAWGKGYGTDAMRVILRYGFTELNLHRVSLDVFEYNPRAIRSYEKAGFKHEGRLRQ
                     YLQREGRRWDLIFMGITESEWYALYSGE"
     gene            complement(2073..>2326)
                     /locus_tag="VN363_05850"
     CDS             complement(2073..>2326)
                     /locus_tag="VN363_05850"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="GNAT family protein"
                     /protein_id="HWQ84069.1"
                     /translation="EVLKLILRYAFMELNLFRLAAVIPEYNTAALSLFQKAGFLEEVR
                     RREALNRYRRRWDLINLGILRSEWQAHAGVENHSPEGSR"
BASE COUNT          508 a          692 c          614 g          512 t
ORIGIN      
        1 agagccacct ggtagataac cgggtcgggc ttcatcagcc ccagctcggc agagattacc
       61 agctcatcga aagcatcccc gatctgccag cgattttcga tcagatggcg caggttggac
      121 cagttattgc tcaataagcc agttttgtac gccggcttga ggcggcggat atagtcaact
      181 aattcataat ccaggcggtc gccgccccaa aacaggcttt gaaattcttt caggctttcc
      241 aatggccagt taagctgcgc gcatacctga gcccaatgct gttcggtact gatcgtgccc
      301 aggctggcca ggagggctga aggactgttg aatacagccg catccagccc ggtataagtc
      361 atgcccaggc ggcgcgccag ctctgtgcgc gggctgcggt ccacagtccg cgccagcacc
      421 ccgcccaggt cccagacgac agctttgatc agatgatgcg cagattcggc cataccatct
      481 ccaatggctt gcgataggca gtgtgccgtt cgacaggctg gaagccgagg ctttcgtaca
      541 ggcgcagcgc cccggaaagg ttttcggtat ccacatgcag ggctgcttcc tgcatacccc
      601 gctcgcgcag cgcctgcagg ctggctgcaa tcagcgcgtg cgccagacct tgcttgcgat
      661 aaggccgccg gacacagata ctctcagtat agcctcgcag acgttgatat tccatgtttt
      721 cagcgcgggt gatatagctt aataccatcc cggctacctg gtcaccctgc caggccacgc
      781 gccacagccc ggggtcgaaa tccgggtctt ccatgagtgc ttccagggtt ggatcgttag
      841 ctgagctgta gccccaatga tcctgaaagg cttcactgct ggccgcaagg atggcaggca
      901 gctgttggtc ggtcaccggc cggatctcat acccgggcgg cagcaccagg tccgggatat
      961 tttccaggtc tggcctgacc atggtgtaga aatggcgggc aggcagataa cccgcagaga
     1021 gcagcaaagc cgtcgtatcc cgttccgtct catcagcgtc ggactggtaa aacatggtct
     1081 gctctgggct gtgggtggcg gcaatcgccc ggatgtgggc ctgcaaatag gccagcactg
     1141 cccggccaat gccccgcccg cgccactcgg gaaggaggaa cccaaaatgg gtgtagatcg
     1201 catccccggc atcttcgaga aaccagatca cccggtaata gccaatgact tcaccattca
     1261 tctcaacgaa gagcatgtct tttgtgatgt caaaattggg ggcatgccgg tagtcacgcg
     1321 caatatcctg ggcagtgtcc gagcgttcca gctggtcgac ctgtttacag ccctggatga
     1381 ccgccaccat tttctcaaaa tcggtttcgt cccgataacc ccgaaagctc aagccaggta
     1441 agtctggcgc gccagggacg aaaatggtaa ttacgctctc ctgtacagta ttcatttcga
     1501 ttattctcct gagtaaagcg cgtaccattc tgactcggtg atccccataa agatcagatc
     1561 ccagcgccgg ccttcacgct gcagatactg gcgcaggcgg ccttcatgtt taaatccggc
     1621 cttttcatag gagcggatcg cacgcgggtt gtattcaaac acatctaatg agacccggtg
     1681 caaatttaac tctgtaaatc catagcgcag gataacgcgc atggcgtctg taccgtagcc
     1741 tttgccccag gcttctcgct caccgaggcc aatcccaacg aaaacatctc catgctgcca
     1801 ctgaaacccc tcgaatccaa caacaccaat gaggcgatcg tcttccaggg tgtggatctc
     1861 aaaaaaaata ctcctggtgg cgtctttttc gagatctttt tccagccatt ccttcgatct
     1921 tttgacagaa aataagtgcg ctgggtcgga tgcctgcagg cgccagtatt cagaatcgcg
     1981 cccccagcga gaatctgctt ccgccaggcg ttggctgtct actgcagtca gacggaccag
     2041 ttttccggta aataaatcct gttccatatc ctttacctgc tgccttccgg gctgtggttt
     2101 tctaccccgg cgtgtgcctg ccactcgctg cgcaggatgc ccagattgat caaatcccaa
     2161 cgcctcctgt agcgatttaa tgcttcccgc cggcgtactt cctcaagaaa gcctgctttc
     2221 tgaaacaggc ttaaggcggc ggtgttatat tccgggatca ccgcagccag acggaacagg
     2281 ttcagctcca taaaggcata gcgcagaatc agcttcagga cctccg
//
LOCUS       DATMXK010000318         4454 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_52368, whole genome shotgun sequence.
ACCESSION   DATMXK010000318 DATMXK010000000
VERSION     DATMXK010000318.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4454)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4454)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4454
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52368"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VN363_05855"
     CDS             <1..350
                     /locus_tag="VN363_05855"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWQ84070.1"
                     /translation="TVPRDIDESAMVEGATHWQIFTRLILPLLRPILVVVGLLSYIGT
                     YGEFVLARVLLKSNENYTLMVGLERFAGAQMSQRWGVFAAGALMGALPIMIIYLILQD
                     QIVGGLTTGAVKG"
     gene            395..2140
                     /locus_tag="VN363_05860"
     CDS             395..2140
                     /locus_tag="VN363_05860"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015926 - glucosidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyl hydrolase 53 family protein"
                     /protein_id="HWQ84071.1"
                     /translation="MKIKSHPGHSKLVVILAVLSLLVGCATPVSQPTAEFTDTPMQAA
                     PTAAAPTAAAPSAADQTVPPLEPGPMTPTPEIEPAEEKATTPEGSDPNADPINLFNPG
                     FETQAPDGLPSGWSHTGAASAVRPDDRGHSGDFRLTHQGTEAYQVETSQQVMGLDNGW
                     YTLRIWVRSSGGQNAVYAALKCGDAEKRVHIPSTSPGYRWLQLVVSNQVTDGQCTISL
                     VSDGLPDTWASFDDLELSPGQAALSILGSDISSLTKSEDLGGIYKDSDGTAQDALQIL
                     KEHGMNYARLRVWVDPADGYHDKDELLEMALRLKSQGIKLLVDFHYSDNWADPGKQIK
                     PAAWKDYDFEQLKQAVYDHTFDVCSSLVAQGTPPEMVQIGNEINSGMLWPDGDYEHFD
                     NLAALLKEGYRAVKECSASTIVMLHIAEGGDNDLARWWFDNITRREVPFDVIGISYYA
                     YWHGSLAELQYNLNDITARYDKDVIVVETAYTFTDQDDDDYPNIATTALAVPNYPLSP
                     AGQRAMLRDIMSIVRAVPNGRGLGVFYWDAVWTAVPGNGWDGSDPTTGNAWENQALFD
                     FEGQVLPALDEFLTP"
     gene            2228..4258
                     /locus_tag="VN363_05865"
     CDS             2228..4258
                     /locus_tag="VN363_05865"
                     /inference="COORDINATES: protein motif:HMM:NF014502.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="beta-galactosidase"
                     /protein_id="HWQ84072.1"
                     /translation="MKLGVCYYPEQWPQERWSEDARLMREAGLKLVRITDFAWSLMEP
                     REGEFTWDWLDRVIETLAAEDLQVILCTPTASPPPWLAKSYPDILHVDEQGRRRRIGS
                     RRHYCVNSPTYQQHTRRIVSAMAARYGEHPAVVGWQIDNELGCHFTGRCYCENCTAGF
                     RTWLAAKYGTIEALNAAWGNVFWSQVYTDWSEIEPPNLTVAGPNPSHVLDYDRFSSAG
                     FVSYVQLQLSVLRPHITEHQIVTTNYMSQFSELDYYEIASLLDLITMSSYPTGHAESN
                     PSLYTPGNFRPSLAYDVGDPYITGMGMTLMRGYKQYYPFWVMEQQCGNVNWGVYNPGV
                     RPGTVRLWTWQALSSGAEAVIYFRWRAGLYAQEQMHSGLLHHDGSADVGYADLHEMLA
                     EQIRLAEIASLPPEARIAILLDFESLWALRVQPHRPDMEYMRQIFLFYRALQRLGLPA
                     DIVSPGGDLTAYRLVVAPAAYIATKDLTRTLQTFAEAGGTVLLGVRSGFKTVDNHVTD
                     RPLPGFLSDLAGITVQEWHSLPPDVSYDFSSAIPGLDGPATVWAEALHPIQSAAAWSE
                     ASTPDFSILAHYTSGPFSSKPALVEHRVGSGRVNYLGWYPTDRQAEILMLYLASTAGI
                     TPTATLPEGVVASQHGPYVVLLNFTDEPQITAVRDQDYTVGPRDVMVVRLES"
BASE COUNT          930 a         1258 c         1280 g          986 t
ORIGIN      
        1 acactgtacc gcgcgatatc gatgagtcag ccatggtgga aggagcgacc cactggcaga
       61 tcttcacccg gctgatactg cctttgctgc gcccgatcct ggtcgttgtc ggcttgctca
      121 gctacatcgg cacctatgga gagttcgtcc tggcccgggt gctgctcaaa agcaacgaga
      181 attacaccct gatggtcggt ctggagcggt ttgctggcgc ccagatgtcc cagcgctggg
      241 gagtatttgc cgctggtgcc ctgatggggg cgctgccgat catgatcatt tacctgatcc
      301 tccaggatca gattgtaggt gggctgacta caggcgcagt caaaggataa tccaggctgc
      361 cggtgagcgt gattctacag gcagggcggc ttctatgaag attaaatcac acccggggca
      421 cagtaagctg gttgttattc tggctgttct gagccttctg gttgggtgtg ccacgccggt
      481 ttctcagccc acagctgaat tcacggatac ccccatgcag gcagccccaa cggcggccgc
      541 cccaacagcg gccgccccat ctgctgccga ccaaacagtc ccacctttgg agccaggtcc
      601 tatgacgcct accccggaaa ttgaacccgc ggaggaaaaa gccacaaccc cggagggctc
      661 cgatccaaac gcagacccca tcaatttatt caaccccggg tttgaaacgc aagccccaga
      721 cggtctgcct tctggctgga gccacaccgg cgcggccagc gccgtccggc cggatgaccg
      781 cggccattcc ggcgatttcc gcctcaccca tcagggaacg gaagcctacc aggtggagac
      841 ctcgcagcaa gtcatggggc tggacaacgg ctggtacacc ctgcgcatct gggttcgttc
      901 cagcggcggg caaaatgccg tctatgccgc cctcaaatgc ggagatgctg agaagcgggt
      961 ccacatacca tccacttccc ccggttatcg ctggttacag ctggtcgtat cgaaccaggt
     1021 cacagacggt caatgcacca tcagcctggt ttcagacggc ctcccggata cctgggcgag
     1081 cttcgatgac ctggaattaa gtccggggca ggccgctctc tcgatcctgg gatcggatat
     1141 atcaagtttg accaagtcgg aagatctggg gggaatttac aaggatagcg acggaaccgc
     1201 gcaggatgcc ctgcaaatcc tcaaggagca tggcatgaat tacgctcgcc tgcgggtctg
     1261 ggtcgatcct gccgatggct atcatgacaa agatgagctc ctcgagatgg cattaaggct
     1321 taaaagccag ggcattaaac tgttggttga ctttcattac tccgacaact gggccgatcc
     1381 tggcaagcaa attaaaccag ctgcctggaa agattacgat ttcgagcagc tcaaacaggc
     1441 tgtctacgat cacactttcg acgtgtgcag cagcctggtc gctcagggga caccgccgga
     1501 gatggtccag atcgggaatg aaattaactc cgggatgttg tggccggatg gcgattatga
     1561 gcactttgat aatcttgctg cgctgctcaa agaaggctac cgggcagtca aggaatgttc
     1621 tgcctccacg attgtgatgc tgcatatcgc cgaaggcggc gataacgatc tggcccgctg
     1681 gtggtttgat aatattaccc gccgcgaggt ccccttcgat gtgattggca tctcttatta
     1741 tgcctactgg catggcagcc tggctgaatt acagtataac ctgaacgata ttaccgcccg
     1801 ctacgataaa gatgtgattg tggtcgagac tgcctatacc ttcaccgacc aggatgacga
     1861 cgattatcca aatatcgcca caacagccct ggctgtcccg aattatcctc tgagcccggc
     1921 aggccagcgt gctatgctgc gcgatatcat gtccattgtc cgggcagtcc ccaacggacg
     1981 cgggctgggc gttttttact gggatgctgt ctggaccgca gtgcctggga acgggtggga
     2041 tgggagcgat cccacaaccg ggaatgcctg ggaaaaccag gctctgtttg acttcgaagg
     2101 ccaggttctg ccagccctgg atgaatttct aaccccatag gcagtgagaa ttttccggta
     2161 aagctccacg caacaaattc aggcagccag agcctgcctc atcctcaacg ctacatgcga
     2221 gcagaaaatg aaacttggcg tttgttatta ccctgaacaa tggcctcaag aacgctggtc
     2281 ggaagatgcc aggctgatgc gtgaagccgg attaaagctg gtgcgcatca cggattttgc
     2341 ctggtcactt atggagccca gagagggtga attcacctgg gattggctcg accgggttat
     2401 tgaaaccctg gcggcggaag atctccaggt tatcctgtgc acgccgacag cctccccacc
     2461 cccctggctg gcgaagtcat acccggacat tctgcatgtg gacgaacagg gccgccggcg
     2521 gcggattggc tcgcgccggc attactgcgt gaacagccct acataccaac agcatacccg
     2581 gcgcatcgtc tcggcgatgg cagcccgcta tggggagcac cctgccgtgg ttggctggca
     2641 gattgacaat gagctgggct gtcatttcac cgggcgctgt tactgcgaga actgcacggc
     2701 tggcttccgc acctggctgg ctgcaaaata tggcacgatc gaagcactca acgcagcctg
     2761 gggaaatgtc ttctggagcc aggtctatac ggattggagc gaaattgagc cgcccaatct
     2821 gaccgttgct ggacccaatc ccagccatgt gctggattat gatcgttttt cgtctgcggg
     2881 tttcgtaagt tatgtgcagc tacaactttc cgttctcagg ccgcacatca ctgaacatca
     2941 aatcgtcacg accaattaca tgagccagtt ttccgagctg gattactatg agattgccag
     3001 cctcttggat ctgattacga tgagctccta tccaaccggg cacgctgaat cgaacccatc
     3061 gttgtataca ccgggcaatt tccggccatc cctggcgtat gatgtgggcg atccatatat
     3121 caccgggatg gggatgaccc tcatgcgggg ctacaagcag tactacccgt tctgggtgat
     3181 ggagcagcag tgtgggaacg tgaactgggg tgtgtataat cccggggtgc ggcccggcac
     3241 ggtcagattg tggacgtggc aggctctttc ttccggtgca gaggcggtca tttattttcg
     3301 ctggagggcc ggcctgtatg ctcaggaaca aatgcattcg ggtttgctcc atcatgatgg
     3361 ctcagccgat gtggggtatg ccgatctgca tgagatgctc gctgagcaga tccggctggc
     3421 tgaaattgcc tccctacctc ctgaagccag gatcgcgatc ctgctcgatt ttgagtcgct
     3481 gtgggcgctg cgggtccagc cgcaccggcc cgatatggag tatatgcgcc agatcttcct
     3541 cttttaccgc gccttacaac gcctgggttt gccagccgat atcgtctctc caggtgggga
     3601 tctcaccgct taccggctgg tcgttgctcc ggctgcctat atcgccacaa aggaccttac
     3661 ccgcaccctc cagaccttcg cagaggcggg gggaacagtg ttgttggggg tgcgctctgg
     3721 atttaagacg gtcgataacc atgtgacaga ccggcctctg ccggggttct taagcgatct
     3781 cgctggcatt acagttcagg aatggcattc acttccgcca gatgtcagct acgatttcag
     3841 ctcggcgatc cccggcctgg atggcccggc cacggtatgg gcagaagcgc ttcatccaat
     3901 ccaatctgca gccgcctggt ctgaagccag cactcccgat ttcagcatcc tcgcccacta
     3961 tacctccgga ccattcagct caaaacccgc tctggttgag cacagggttg ggtccggacg
     4021 ggtgaattat ctgggttggt atccgaccga ccggcaggct gaaattctta tgctctatct
     4081 ggcctcaact gcaggcatta ccccgacagc cactctgcca gaaggggtcg tcgcgtctca
     4141 gcatggtccg tatgttgttt tgctcaattt cacagatgag ccacagatca cagcggttcg
     4201 agatcaggat tataccgtcg ggcctcgcga tgtgatggtt gtcaggcttg aatcctgagt
     4261 gaccttgctc ctggagggat aggtatggca cggaagcagc ctctgttggt gcgagcgtac
     4321 cgggaaggcg cgccgttgat cgatggcgag taggttacat ttctgtggga agggaaaaca
     4381 gcgccatctt tgcgtgggga cttcacaggc tggcgagagg gtgagtcctt gagccttact
     4441 cgcctggcgc ccgg
//
LOCUS       DATMXK010000319         5585 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_109173, whole genome shotgun sequence.
ACCESSION   DATMXK010000319 DATMXK010000000
VERSION     DATMXK010000319.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5585)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5585)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5585
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109173"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..620
                     /locus_tag="VN363_05870"
     CDS             <1..620
                     /locus_tag="VN363_05870"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ84073.1"
                     /translation="KNLTPPQTLDDLLAPEYRSLLVVENPANSSPGLAFLLATIGRYG
                     EDGYLDYWRGLVENDVLVVNDWETAYNSEFSYWGGTRPFVVSYSSSPPFEMIFAETPL
                     AEPPTGTVTGAQSCFRQIEFAGILKGTPNRDLAEAWIDFMLSPTFQEDMPLQMAVFPV
                     NPQAALDESFADWLETPQQPAMVDPELIASQREAWIEAWTEAVLR"
     gene            659..2398
                     /locus_tag="VN363_05875"
     CDS             659..2398
                     /locus_tag="VN363_05875"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron ABC transporter permease"
                     /protein_id="HWQ84074.1"
                     /translation="MPSGTPSGPASRPPVSRLQWLLWIAPLVFLALFYFYPLGSILGL
                     SFARQEGGWAAAFTRALSSANVWRVLGFTLGQALLSTFLTLLLGLPGAYLLARYQFRG
                     KSLIQALTGLPFVMPTLVVAAAFNALLGPRGWVNAGLMSLFDLAQPPLQFTNTLTAIL
                     VAHVFYNTTIVLRMVGDFWSHLDPRLEQAAQTLGANRWQTLRHVTLPVLQPAVLAAAL
                     LVFIFDFTSFGVVLVLGGPRFATLEVEIYYQTVSLFNLPLAAVLSLIQLITTLGMTLL
                     YSRLNARLARPLALKPRQITQHRLQGWRARLLAGAFLVFLLAFMTLPLLGLAGRSVYN
                     LNPGRERTGEVAPGPTLAYYRALSESRRSALFYAPPATSIAISLGYAAATVVLAVGLG
                     LPAALALTTRREGRVSRLLDPIFMLPLGTSAVTLGLGFIVALDQPPLDLRASPLLLPL
                     AHTLVAFPFVVRSLAPSLASINPRLRQAAAVLGASPAQVLRKIDLPLVGRALLVAATF
                     AFTISLGEFGATALVSRPEYPTIPAAIYRLISQPGALNYGQAMALSTILMVVSAAGML
                     FIERMRIADVGEF"
     gene            2407..3534
                     /locus_tag="VN363_05880"
     CDS             2407..3534
                     /locus_tag="VN363_05880"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84075.1"
                     /translation="MPGQTMQGLEVEHISRSFGSTRAVQDVSFQVAPGEIVAVLGPSG
                     CGKSTVLSIVAGLLAPDSGEVVWDGQSLKNVPPYRRGFGLMFQDFVLFPHRNVFENVA
                     FGLQIHGQPSHEVYQRVSEALELVGLPGFDKRDVNTLSGGEQQRVALARSLAPRPRLL
                     MLDEPLGSLDRALRERLVEDLRLILRRLKQTALYVTHDQEEAFVLADRVVLMNAGRVE
                     QIGSPAELYNHPATEFVARFLGLDNLLPGRYGCDPSGCWVETDLGRWAILQGPPVVHG
                     TPAPQGTPGESRNVTVLLRPDAVQLDSQGDCSLAGTVSEVVFKGRSTRLELEVNGTHL
                     SFDFPSRSGLPRPGEPVCLSFNPEEALQLFQGSQRDGNAGI"
     gene            3521..4036
                     /locus_tag="VN363_05885"
     CDS             3521..4036
                     /locus_tag="VN363_05885"
                     /inference="COORDINATES: protein motif:HMM:NF016085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF402 domain-containing protein"
                     /protein_id="HWQ84076.1"
                     /translation="MPESDQLTVLKLKPTGELIWSYSGRLLELAGSRLVLEAFFDLED
                     RLKEGMLFAKGDRFIETYYSDRWYNIFTIYSRGDDSLRGWYANIARPALFERNTVSYV
                     DLALDLLVFPDGRQVVLDEDEFVELDLAAEEKAAARQALAELQSSFRDYLAGSEKKGL
                     RSGNYSTGRSD"
     gene            complement(3999..4202)
                     /locus_tag="VN363_05890"
     CDS             complement(3999..4202)
                     /locus_tag="VN363_05890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84077.1"
                     /translation="MDPFEPLPIPPPLEEPKRKTNTALIIVIVVLVILCCLCLVGGFG
                     IYWLWENGDAIFNLTGLLNNFLI"
     gene            complement(4263..4823)
                     /locus_tag="VN363_05895"
     CDS             complement(4263..4823)
                     /locus_tag="VN363_05895"
                     /inference="COORDINATES: protein motif:HMM:NF024937.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4126 domain-containing protein"
                     /protein_id="HWQ84078.1"
                     /translation="MTGIFTAFGLSASAGLNAYIPLLVVALLARFTDLIKLNSPWDTL
                     ASPWIIGLLVLLSTVEFFADKIPAVNHVNDAIQTVVRPAAGAIAFAASAQVITDIHPV
                     LSLAAGLLVAGTIHTIKSAAVRPAVTATTAGAGNIPVSIAEDVTATVISILAVVIPLI
                     LAVIVILLTSWVVLRMWQRANRTHSA"
     gene            complement(4937..>5585)
                     /locus_tag="VN363_05900"
     CDS             complement(4937..>5585)
                     /locus_tag="VN363_05900"
                     /inference="COORDINATES: protein motif:HMM:NF021384.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2085 domain-containing protein"
                     /protein_id="HWQ84079.1"
                     /translation="GERQLPLCARCSGMYLGAVLGLGFQAWLKPRRSATPPRRVLIFL
                     GLLVAAFALDGVNSFLHLIPGTFRLYEPQNWLRLFTGSGMGLTLAALLYPAFNGTVWT
                     HPDPRPALDRLGGLSGLGSLAGLTALLNLLVLIENPVVLYPLALISAGGVLLVLTLVY
                     SLVWLMVARRENRFERFSQAWIPLLGGFGLALLQIAALDWLRFLLTGTWSGFIMG"
BASE COUNT         1037 a         1663 c         1675 g         1210 t
ORIGIN      
        1 agaaaaatct caccccgccg caaaccctgg atgatctcct ggctccggaa tatcgcagcc
       61 tgcttgtcgt ggagaacccc gcaaattcct cgcccggatt ggcgtttctg ttggcgacca
      121 ttgggcgata cggcgaagac ggctacctgg actactggcg cgggctggtt gagaacgatg
      181 tgctcgtggt gaacgattgg gagacagctt ataacagcga attttcctat tggggcggca
      241 cccgcccctt cgtggtctcc tacagctcca gccctccgtt tgagatgatc ttcgccgaga
      301 ctcccctggc agagccgccg acaggcacgg ttaccggcgc gcagagctgt tttcggcaga
      361 tcgagtttgc aggtatcctg aagggtaccc cgaaccgcga tctggctgaa gcctggattg
      421 atttcatgct ctctcccaca ttccaggaag atatgcccct ccagatggca gtcttcccgg
      481 tcaacccgca ggccgcgctg gatgaatctt ttgcagattg gctggaaacg cctcagcagc
      541 cggccatggt cgatcccgaa ttaatcgcca gccagcgtga agcctggatc gaggcctgga
      601 ctgaggccgt cctgcgttga gccagcctgc gccagcccca gcagccagac aggaccgtat
      661 gccatccggc acgccgtccg gtccggctag ccgccctccg gtgagccggt tgcaatggtt
      721 gttgtggatc gcaccgctgg tcttcctggc cctgttttat ttctacccgc tgggcagcat
      781 cctgggcttg agctttgccc gccaggaggg tggctgggca gcagcattca cccgggctct
      841 gtcttcagcg aacgtatggc gtgtgcttgg ttttaccctg gggcaggctc tgctctctac
      901 tttcctgaca ttgctgcttg ggctgccggg agcgtatctg ctggctcgct accagtttcg
      961 cggaaaatcg ttgatccagg cgctgaccgg cctgccattc gtcatgccca ccctggtggt
     1021 ggcagccgcc ttcaatgccc tgctgggtcc gcgcggttgg gttaatgccg ggctgatgag
     1081 cttatttgac ctcgcccaac ctcctctcca gttcaccaac acgttgactg ccattctggt
     1141 ggcgcatgtc ttttataaca ccaccatcgt gctgcgtatg gtaggcgatt tttggtcaca
     1201 cctcgacccg cgcctcgagc aggctgccca gaccctgggt gctaatcgct ggcaaaccct
     1261 gcgccacgtc accctgccgg tattacagcc ggcagtcctg gctgccgcgc tcctggtatt
     1321 tatctttgat ttcacttcct ttggtgtggt gctggtcctt ggcgggccgc gttttgccac
     1381 cctggaagtg gagatctatt accagacggt aagcctgttc aacctgcccc ttgcggcggt
     1441 gctgtccctg atccagctga taaccacgct ggggatgact ttgctgtatt cgcggctcaa
     1501 cgctcgcctc gcccgcccgc tggcgctcaa accgcgccag atcacccagc accgcctgca
     1561 gggctggcga gcgcgcttgc tggcaggagc ctttctggtc ttcctgctgg cattcatgac
     1621 cttacccctg ctcggtctgg ctggacgctc ggtttacaat ctgaatcccg gccgcgaacg
     1681 cactggcgaa gttgcccctg gaccgacgct ggcttactac cgggcgctga gtgaatcgcg
     1741 ccgctcggcc ttattttatg cgcctcccgc cacctcgatc gccatctccc tgggatatgc
     1801 tgccgccacg gtagtcctgg cagttggcct cggcctgcca gcagccctgg cgctgaccac
     1861 ccggcgggag ggtcgggtca gccgcctgct cgatccgatt ttcatgctgc cgctgggtac
     1921 ttcggcggtc acgctgggat tgggtttcat cgtcgccctg gaccagccgc cgctcgattt
     1981 gcgcgcctca cccttgctgc tgcccctggc gcacaccctg gtcgcattcc cgttcgtggt
     2041 acgcagcctg gcgccttccc ttgccagcat caatcctcgc ctgcgccagg ctgcggcagt
     2101 gttaggcgcc agcccggctc aggtgctgcg caaaattgat ctgcccctgg tcgggcgcgc
     2161 tttgctggtc gcagcgacat ttgcctttac catctccctg ggtgaatttg gtgcaacggc
     2221 gctcgtctct cgcccggaat accctacaat cccggcggcg atataccgcc tcatctcgca
     2281 gcctggcgcg ctgaactatg ggcaggccat ggcgctgtcc acgatcttga tggttgtcag
     2341 tgcggcgggg atgcttttca tcgaacgcat gcggatcgcc gatgtcggcg agttttagga
     2401 gcagccatgc ccggtcagac catgcaggga cttgaagtag agcacatcag ccgcagtttt
     2461 ggcagtaccc gcgcggtgca ggacgtcagc tttcaggtgg ctcctggcga gatcgtggcc
     2521 gtgcttgggc cgagcggttg tggaaaatcc actgtgcttt ccattgtggc gggtcttctg
     2581 gcgcccgatt cgggagaagt agtctgggac gggcagtcgc taaagaatgt acccccttac
     2641 cggcgtggtt ttgggcttat gttccaggat tttgtgctct tcccccaccg gaatgtgttt
     2701 gagaatgtgg ctttcgggct gcagatacat gggcagcctt cgcatgaggt ctaccagcgg
     2761 gtgagcgagg ccctcgagct cgtgggtctg cccggttttg ataaacgcga tgtcaatacc
     2821 ctgtcgggag gcgagcagca gcgcgtggcc ctggcgcgct cgctggcacc tcgcccgcgc
     2881 ctgttgatgc tggatgagcc tttgggctcc ctggatcgcg ccttgcgtga gcgcctggtc
     2941 gaagacctgc gcctcatcct gcgccgcctg aagcagaccg cgctgtatgt cacccacgat
     3001 caggaggaag ctttcgtcct ggccgaccgg gtggtgttga tgaatgccgg gcgggtggaa
     3061 cagattggtt ccccggccga attatataat cacccggcta cggagttcgt ggcgcgcttt
     3121 ttggggctgg ataacttgct tccgggtcga tacggctgcg atccctccgg ctgctgggtt
     3181 gagactgatt tggggcgctg ggctattctg caaggcccgc ctgtagttca cggcacgcct
     3241 gctccccaag gcacgccagg ggagtccagg aatgtaaccg tcctgctcag gccggatgca
     3301 gtgcagctgg acagccaggg ggattgcagc ctggcgggga cggtcagcga ggtggttttc
     3361 aaaggccgct ccacgcgcct ggagctggag gtgaatggaa cccacctgag cttcgatttt
     3421 ccgtcgcggt ctggcttgcc ccgcccgggt gagcccgtct gcctgagctt taatcctgaa
     3481 gaggctttac aattatttca aggatcgcaa agggatggca atgccggaat ctgaccagct
     3541 gaccgtactg aagctcaaac cgaccgggga gcttatctgg agctattctg gtcgtctcct
     3601 ggaactggct ggctcgcggc tggtcctgga agcttttttc gatctggagg atcgtctgaa
     3661 ggagggtatg ctgtttgcta agggcgaccg cttcatcgag acgtattact cagatcgctg
     3721 gtacaacatc ttcaccattt acagccgcgg cgatgacagc ctgcggggat ggtatgcgaa
     3781 tatcgcccgc ccggcgcttt tcgaacgaaa taccgtatcg tatgtcgatc tggcactgga
     3841 tttgctggtt tttccagatg ggcggcaggt tgttctggac gaggatgaat ttgtggaatt
     3901 agacctggca gcggaagaaa aagctgctgc ccgccaggcg ctggccgagc tgcaatccag
     3961 ctttcgcgat tacctggcag gcagcgaaaa aaagggactc agatcaggaa attattcaac
     4021 aggccggtca gattaaagat cgcatcgccg ttttcccaca gccagtaaat cccaaagcct
     4081 cccaccaggc ataagcagca caaaatgacc aggaccacga tgacgatgat caatgcggta
     4141 ttggttttcc tttttggttc ttctaatggg ggtggtattg gaaggggttc gaaggggtcc
     4201 atatttattc tcctcaatca tgccaaagcc agccggccgc agcggcgtgc gagccggaga
     4261 gatcaagccg agtgggtgcg gtttgctcgc tgccacatgc gcagcaccac ccagctggtt
     4321 aataaaatga cgatcactgc cagaatgagc gggattacca cagccaggat ggatatgact
     4381 gtggcggtta catcttctgc gatactgacc ggaatattgc ctgctccggc ggtggtcgcg
     4441 gtgactgcgg gacggaccgc ggcggatttg atcgtgtgaa ttgtaccggc taccaataag
     4501 ccggcggcca gcgagagcac gggatggata tcggtgatca cctgggcgct ggctgcgaag
     4561 gcaattgccc cggctgccgg gcgcaccact gtctggatgg cgtcattgac gtgattgacg
     4621 gccgggattt tatcggcgaa gaactcgacc gtggaaagca gcaccaataa tccgatgatc
     4681 caggggctgg ccagggtatc ccagggtgag ttgagcttga ttaaatcggt gaaccgcgcc
     4741 agcagggcga caaccagcaa ggggatgtat gcattcagcc cggcgctggc tgaaagccca
     4801 aatgctgtaa aaattccggt caaaatctcc aaatttcctc cgagaatgga cggcgcaatc
     4861 tcatctagtt tattatacgt ggacttcagg aaaaggtttc agcggattac agctgggtcg
     4921 aatttaagaa catcttctaa cccattatga atccactcca ggtgccggtg agcaaaaagc
     4981 gcagccagtc cagggccgca atctgcagca gtgccagccc gaagccgcct aacaggggta
     5041 tccacgcctg agaaaagcgc tcgaagcgat tttctctgcg agctaccatc aaccatacca
     5101 ggctgtacac cagggtcagt accagcagca cacccccggc gctgataaga gccagtgggt
     5161 agaggacaac cggattttcg atcaaaacca gcaggttgag cagcgctgtc agtccggcca
     5221 gggaccccag accactcagc ccgcccaacc ggtcgagcgc cgggcggggg tcgggatgcg
     5281 tccagactgt tccattaaaa gccgggtaaa gcagcgctgc cagggtcaaa cccatcccgc
     5341 tcccggtgaa cagccgcagc cagttctgcg gctcatacag ccggaaggtt cccgggatca
     5401 ggtgcaggaa tgaattgacc ccatcgagag caaacgctgc caccagcagc cccaggaaaa
     5461 ttagcacccg ccggggaggc gtcgccgacc gccgcggctt cagccaggcc tggaaaccca
     5521 atcccaggac tgctcccagg tacatccctg agcagcgggc gcagagtgga agctggcgtt
     5581 cgccc
//
LOCUS       DATMXK010000320         3027 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_47192, whole genome shotgun sequence.
ACCESSION   DATMXK010000320 DATMXK010000000
VERSION     DATMXK010000320.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3027)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3027)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3027
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_47192"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(83..838)
                     /locus_tag="VN363_05905"
     CDS             complement(83..838)
                     /locus_tag="VN363_05905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84080.1"
                     /translation="MVAASLTTGDYAPIGLANETRAELSPILADLFPLRLYTRLLLRG
                     VDGYISLLAHESYHTFAGIQAPEKLAQAERAARLANSYPWEEQPLIDAWREELDLLSL
                     GAEAAWGEASEAEVLDMAQRFLAQRAERRLNLGLSPELIAYEQQCEWSEGLARYVELD
                     IWRQAYLKKDYQPLPETLALPDFNAYQGFPQRLAQEIDQIPRMASNEGDGRFYYSGFA
                     QAAMLDRLLPGWKERIMQEGVWLDDLLNEAGGG"
     gene            complement(783..1016)
                     /locus_tag="VN363_05910"
     CDS             complement(783..1016)
                     /locus_tag="VN363_05910"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84081.1"
                     /translation="MSLKNAPMLTLTEEEDPPGGVVGRQRRAACVIQKTPLYAQAIGV
                     SPEVSHGIPGSEKSQDGSRQPDHRRLRPDWPGK"
     gene            1077..1430
                     /locus_tag="VN363_05915"
     CDS             1077..1430
                     /locus_tag="VN363_05915"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HWQ84082.1"
                     /translation="MKKVAFPSNDSQTIHHHLGEANLLMVAHLEEDSLTGQFEQRVKT
                     QVAEECGHHQPIFRMLSDCQVVIAGGMCRNAFKHLNEMGLEVILTGETDIHKALDTYR
                     RGEMVSETGRIIAGH"
     gene            1515..2702
                     /locus_tag="VN363_05920"
     CDS             1515..2702
                     /locus_tag="VN363_05920"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWQ84083.1"
                     /translation="MQNLIRELFLLDPEIIFLNHGSFGACPKPVFEIYQSWQRRLERQ
                     PVKFLGREIRELDCTARQALASFLHTRAENLAFVPNATHGVNIIARSLGLEPGDEILT
                     SDHEYGACDHTWEFIAQKTGARVVRQHIPLPGALATTGIQGTPEADITELIWQGVTPR
                     TRLIFLSHITSPTAMRLPVEVICALARAAGIRTLIDGAHAPGQIPLDLEAVGADWYTG
                     NCHKWMLAPKGSGFLYARPEVQSLVEPLVVSWGYHAAPETTSGSQFLDYMVWTGTGDP
                     AAYLTVPAAIQFMQEHAWETVRLDCHHLLRETIGEICALTDIQPAYPLDSDLYFQMGT
                     APLPAGINPTDLKNRLYDEYRIEVPITEWNGQKFLRISIQAYNTPADTAALLEALQEL
                     LVK"
     gene            2778..>3027
                     /locus_tag="VN363_05925"
     CDS             2778..>3027
                     /locus_tag="VN363_05925"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HWQ84084.1"
                     /translation="MTIHPPFTYTILGAGRQGAAAAYDLGRFGEAERILLADASLSQA
                     QKTARRLNALLPQPVVEARQLDVSDPDGLRQALGPADVA"
BASE COUNT          639 a          937 c          846 g          605 t
ORIGIN      
        1 ccgcattgca gtgcggttgc aaccccgcgc caattggctt gccaaattgc tggccgaatg
       61 gccagcaatt agacttgcgt tctcatccgc caccagcctc attcagcagg tcgtcgagcc
      121 agacaccctc ttgcataatc cgctctttcc atcccggcag caggcggtcc agcatggctg
      181 cctgggcaaa gcctgaataa taaaaccgcc cgtcaccctc gtttgaggcc atgcgtggaa
      241 tctggtcgat ctcctgcgca aggcgttgag gaaaaccctg gtaggcattg aaatctggca
      301 gcgccagagt ttctggcagg ggctggtaat ccttcttcaa gtaagcctgc cgccagatat
      361 ccagctccac gtagcgagcc aggccttccg accattcgca ctgctgttcg taggcgatca
      421 attcggggga taatcccagg ttcaggcggc gctcggcgcg ctgggcgagg aagcgctggg
      481 ccatgtccag cacttcagct tcggacgcct cgccccaggc tgcctcggcg cccagggaga
      541 gcaggtccag ctcctcacgc caggcatcga tcaagggctg ctcctcccag ggatagctgt
      601 ttgccagccg ggcggcgcgt tctgcttgag ccagcttctc tggcgcttga atgccagcaa
      661 aggtatggta gctttcgtgc gccaaaaggc tgatatagcc atccacgccg cgtaataaga
      721 gccgtgtgta caggcgcagc gggaacaggt ctgcaaggat gggtgaaagc tcagcgcggg
      781 tttcatttgc caggccaatc ggggcgtaat cgccggtggt caggctggcg gctaccatcc
      841 tggcttttct cgctgccggg tatgccgtgg ctaacttctg ggcttacacc gatagcctgg
      901 gcgtagagag gtgttttctg gattacacag gctgcccgcc gctgcctccc gaccaccccg
      961 cctggtggat cttcttcttc agttaaggtg agcatcggcg catttttaag tgacatctgg
     1021 aatgtattca aattgggcat acccgatata ctttgccagg aaaggtgaga acacagatga
     1081 aaaaagtagc attcccatcc aacgatagcc agaccatcca ccaccattta ggggaggcca
     1141 acctgctcat ggtcgcccac ctggaagagg acagcctaac cggacagttt gagcagcgcg
     1201 tgaaaactca ggtcgctgaa gaatgcgggc accaccaacc gattttccgc atgctcagcg
     1261 actgccaggt ggtgatcgcc ggcggcatgt gccgcaatgc cttcaaacac ctgaacgaga
     1321 tgggcctgga agtgatcctg accggtgaga ccgacatcca caaggcgctg gatacttacc
     1381 ggcgcggcga gatggtttcc gagaccgggc ggatcatcgc cgggcattaa tatatcggtt
     1441 gttttatccc tcagacccca ggggttgaaa tcctgcggat ttattaaacc cctggggtct
     1501 ggtaatatcg cagtatgcag aacttaatcc gcgagctctt cctgctcgat cccgagatca
     1561 tctttctcaa ccacggctca tttggcgcct gccccaaacc agtatttgaa atctatcaaa
     1621 gctggcagcg ccgcctggaa cgccaaccgg tgaagttcct gggtcgcgaa atccgcgagc
     1681 tggactgcac ggcgcgccag gcactggcgt ctttcctgca tacccgggct gaaaacctgg
     1741 ctttcgtccc caacgccacc cacggggtga atatcatcgc ccgctcgctg ggacttgagc
     1801 ccggcgacga gatcctgacc agcgatcatg agtacggggc ctgcgatcac acctgggaat
     1861 ttatcgctca gaaaaccggg gccagggtag tccgccagca tatcccgctg ccaggagcct
     1921 tggccacgac cggaatccag ggtacgcccg aagcggacat caccgagctg atctggcagg
     1981 gcgtgacccc gcgcacaaga ctcattttcc tgtcccacat cacctcccca accgctatgc
     2041 gcctgccggt cgaagtgatc tgtgccctgg cgcgcgcggc tggcatccgg accctgatcg
     2101 atggcgccca cgctccgggc cagatcccgc tggacctgga ggcggtaggc gccgactggt
     2161 ataccggcaa ctgccacaaa tggatgctgg cgccaaaagg cagcggtttc ctgtatgccc
     2221 gtccagaagt ccagtccctg gttgagccgc tggtagtcag ctggggctac cacgccgcgc
     2281 ccgagacaac ctcaggctct caatttctgg attacatggt ctggaccgga acaggcgatc
     2341 cggcagctta cctgaccgta ccggcagcca tccaattcat gcaggaacac gcttgggaga
     2401 ctgtccgcct ggattgccac cacctgctgc gggagactat tggagaaatt tgtgccctga
     2461 cagacattca gcccgcttac ccgctcgact cggatctgta ctttcagatg ggtactgcgc
     2521 ccctgccagc cggaatcaat cccactgatc taaaaaatcg cctgtatgac gaatatcgca
     2581 tcgaggtccc gatcaccgaa tggaatggac agaaattcct gcgtatctcg atccaggctt
     2641 acaacacgcc cgctgatact gctgccctgc tggaagccct tcaagaactc ctggttaaat
     2701 aatccggatt aatttcccag acctgatcca gagcgtataa ttatccccgg acacaaaacc
     2761 agcacaagga gccgggaatg accatccacc cacctttcac ctataccatt ctgggcgccg
     2821 ggcgccaggg cgcggctgca gcctacgatc tgggcaggtt tggcgaagca gaacgcatct
     2881 tgctggcgga tgccagcctg tcccaggcgc aaaaaactgc ccggcgcctg aacgcactgc
     2941 tccctcagcc ggtggttgaa gcccgccagc tggatgtgag cgacccggat ggcttacgcc
     3001 aggcactggg tccggcggat gtagctc
//
LOCUS       DATMXK010000321         3635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_11779, whole genome shotgun sequence.
ACCESSION   DATMXK010000321 DATMXK010000000
VERSION     DATMXK010000321.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11779"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1264
                     /locus_tag="VN363_05930"
     CDS             <1..1264
                     /locus_tag="VN363_05930"
                     /EC_number="3.4.22.70"
                     /inference="COORDINATES: protein motif:HMM:NF016119.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004197 - cysteine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0016485 - protein processing [Evidence
                     IEA];
                     GO_process: GO:0045184 - establishment of protein
                     localization [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sortase"
                     /protein_id="HWQ84085.1"
                     /translation="ARVGSNFTFTIRVTNNGSGTATDVTVRSSYSTYETISSANSTKG
                     TTNTSASTRTVTGNIGNLAPGESATVTVVTRVSTSATSTVTLTNAATVTYRFSSNNFS
                     RTSNSVTYRVTGSSTLPSTGFMEADPPDDGPAGTAAWIGGLLAVLGAGALLYSLYARK
                     HNPYWTDWAVKTGAVLLIVGLVFGGVAWSLRPIPEAASLAQLLATPPEDYARAPYRPN
                     NPPEAPVIDPQYPFPGGPDELESLPNFTIPTPTVIITPAAEGSAVKPPDTTAITRLVI
                     PALGVDNIVKYVPYDGATWLISGLQDEIAWMGDTSWPGLGSNTALAGHVTLRDGSDGP
                     FRNLKDLMAGDVVKVYTEENVYTYQVREQRIVNDYDLSAVSASEASQLTLITCSGWDS
                     EIRLYISRLVVASDLVEVNPLDRAVLGN"
     gene            complement(1359..3020)
                     /locus_tag="VN363_05935"
     CDS             complement(1359..3020)
                     /locus_tag="VN363_05935"
                     /EC_number="3.5.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase"
                     /protein_id="HWQ84086.1"
                     /translation="MPWSDGSLASIELYPGKPALMLWITNANIITLDASQPAVRALLI
                     DRGRVIAIGEGADHLAANLLDVEKLDAGGRTILPGLTDAHIHLELYALGLQKINCETD
                     TKAECLQRVAERTAGSAPGEWILGHGWNQNVWADGFGNLTDLDQIAPHNPVYLTAKSV
                     HAAWANSAALRLANLTDTSPDPDGGSLGRDAQGRLTGILFENAMGLVERTIPEPQIDH
                     VEAAIREAMPVFWRFGLTGVHDFDRRRCFMALQNLRQQGELKLRVIKSVPIDELPHAV
                     ALGLQSGFGDEFLTIGSVKGFADGALGPQTAAMLAPYENSPQNRGMLLLDAEELFERG
                     RPAVENGLSLAIHAIGDRANHEVLKAYAQLRAVEASLPGYQPERRLRHRIEHVQVIHP
                     QDIRRLAELGVIASMQPIHATSDMLIADRYWGSRAALSYAWRTQQGAGATLAFGSDAP
                     VESPNPFWGLHAAVTRRRLDGSPGVDGWYPEQRLTLLEALHGFTTGPAYAAGREDRQG
                     RLAAGYFADLLVLDEDPFTIPPEALAELAPVGTLLGGEWVYRDFE"
     gene            complement(2986..>3635)
                     /locus_tag="VN363_05940"
     CDS             complement(2986..>3635)
                     /locus_tag="VN363_05940"
                     /inference="COORDINATES: protein motif:HMM:TIGR00180.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA];
                     GO_process: GO:0007059 - chromosome segregation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ParB/RepB/Spo0J family partition protein"
                     /protein_id="HWQ84087.1"
                     /translation="SGDQPGTYILIAGERRLLAARQIGLRKVPVIVREASEQEHLELA
                     LIENLQRANLNPLEAAEAYRQLAEEFNLSHEEIALQVGKKRSTVTNTLRLLNLTAVAR
                     QALTAGRISEGHARILLALSTPQAQEAALQTILTKDLSVRQTEELVRLLNGHKAPRSE
                     RPGPGPEVLALEERLRTSLGTRVTLNQHRKGGTLVIHYYSDEELDALVGRITGID"
BASE COUNT          722 a         1139 c         1028 g          746 t
ORIGIN      
        1 ggcgcgagta ggatcgaatt tcacctttac gatccgggtt accaacaatg gcagcggcac
       61 agcgaccgat gtaaccgtac gcagctctta ttcgacgtat gaaacgattt ccagtgcgaa
      121 cagcaccaaa gggaccacca ataccagcgc ctccacccgc acggtgaccg gaaatattgg
      181 caacctggca cccggcgaaa gcgcgacggt gacggtagtt acccgcgtca gtacttccgc
      241 caccagcacc gtcaccctga cgaacgccgc cacggtcacc tatcgcttta gcagcaataa
      301 tttctcccgc acttccaaca gtgtcaccta ccgggtgacc ggttcttcca cgctgcccag
      361 caccggattc atggaagcag atcccccgga cgatggtccg gcaggcactg cagcctggat
      421 cggaggcctg ctggctgtac tgggggcagg agccttgctc tacagcctgt atgcccgcaa
      481 gcacaatcct tactggaccg attgggccgt caagactgga gcagtattac tgattgttgg
      541 tctggttttt gggggtgtcg catggagcct gcgccccatt cctgaggcag cctccctggc
      601 ccagctgctg gcgacgccgc ctgaggatta tgcgcgcgcc ccgtatcgtc cgaacaatcc
      661 acccgaagcg ccggtaattg acccacagta ccccttcccg ggtggtccgg acgaattgga
      721 aagcctgccc aatttcacca tccctactcc caccgtgatc atcaccccgg ctgccgaggg
      781 ttcagccgtc aagcctccag acacaaccgc gatcacccgc ctggtgatcc ctgccctggg
      841 cgtggacaat atcgtgaaat atgtccccta cgatggcgct acctggctga tttccggctt
      901 acaggatgag attgcctgga tgggggatac ttcctggcct ggtttgggca gcaatactgc
      961 cctggcaggc catgtcaccc tgcgggatgg ctcggatggg cctttccgca accttaagga
     1021 cttaatggcg ggtgatgtcg ttaaagttta caccgaggag aacgtttaca cctaccaggt
     1081 ccgcgagcag cgcatcgtca acgattacga cctttcagcc gtcagcgcca gtgaggcgtc
     1141 gcagttgacc ctgatcacct gctctggctg ggattcggaa attcgcctgt atatctcccg
     1201 tctggtggtc gccagcgatc tggtagaagt gaatccgctg gaccgggctg tgttgggcaa
     1261 ttaagcgcag tctcttggat gcatcgttat caaacggaaa cgccccgagc agatcaagct
     1321 ccggggcgtt tttgttttct gagaattatt tagcaagctc attcaaaatc acgataaacc
     1381 cactccccac ccagcagggt gcccaccgga gcaagctccg ccagggcttc gggcgggatg
     1441 gtaaacggat cttcatccaa caccagcaga tcggcaaaat aacccgctgc caggcgtccc
     1501 tggcgatctt ctcgcccggc ggcataagcc gggccggtgg taaatccatg cagtgcttcc
     1561 agcagggtca ggcgctgttc cggataccag ccatccacgc ccgggctgcc atccaaacgc
     1621 cggcgggtca cggcggcatg cagcccccag aagggattgg gggattcaac gggcgcatcc
     1681 gagccaaatg ccagggtcgc ccctgctcct tgttgagtgc gccaggcata cgaaagcgca
     1741 gcccggctgc cccaataacg atcggcgatg agcatatctg aggtagcatg gatgggctgc
     1801 atcgaagcaa tcacccccag ctccgccagg cggcggatat cttgaggatg aatcacctga
     1861 acgtgctcaa tgcggtggcg caggcggcgc tccggttggt agcccggtaa acttgcttcg
     1921 acggcccgca gctgggcata ggctttcagg acttcatggt tggcacgatc gccgatggca
     1981 tggatggcca gactcaagcc attttcgact gccgggcggc cgcgctcgaa cagctcttcg
     2041 gcatcgagca gcagcatacc gcgattctgc gggctgtttt cgtacggcgc cagcatcgcc
     2101 gcagtttgcg gccccagggc cccatccgcg aagcctttga ccgaaccgat ggtgagaaat
     2161 tcgtccccaa atccgctttg caagcccaga gcaactgcat gcggcagctc atcgatgggc
     2221 acacttttga ttacccgcag cttaagctcg ccctgctgac gcagattttg cagcgccatg
     2281 aaacagcgcc gccggtcaaa atcatgcact ccggtcaggc caaagcgcca gaataccggc
     2341 atcgcctccc ggatcgccgc ttccacatgg tcgatctgcg gctcggggat agttcgctcc
     2401 accaggccca tggcattttc aaacaggatc ccagtcagtc tgccctgagc gtcccgtccc
     2461 aggctgccgc catctggatc ggggctcgtg tctgtgagat tcgccaggcg cagcgcagcg
     2521 ctgttcgccc aggcggcatg cacagatttg gctgtcagat aaaccggatt gtggggcgcg
     2581 atctgatcca ggtctgtcag attaccaaaa ccatctgccc agacattctg gttccagcca
     2641 tgccccagga tccattctcc cggggcgctg ccggcagtcc gttcagccac tcgctgcagg
     2701 cattcggcct tggtatctgt ctcgcagttg atcttttgca ggcccagcgc ataaagttcc
     2761 aggtggatat gggcatcggt taacccgggg aggattgtgc gcccgcctgc atcaagcttt
     2821 tccacatcga gcagattggc agccaggtgg tcggcgcctt ctccgatggc aattaccctc
     2881 cccctgtcaa tcaacagggc gcggacagcc ggctggctcg catccagcgt aatgatattg
     2941 gcgttggtta tccacaacat gagggcaggt ttcccggggt aaagctcaat cgatgccagt
     3001 gatccgtccg accagggcat ccagctcttc atccgaataa taatggatca ccaatgtgcc
     3061 gcctttgcgg tgctggttca gcgtgacccg ggtacccagg ctggtgcgca ggcgctcttc
     3121 aagcgccagg acttcggggc caggtcctgg gcgctccgag cggggagctt tatgcccatt
     3181 gagtaaccgc acgagctctt ccgtctggcg cacgctgaga tccttcgtca ggatcgtttg
     3241 cagggcagct tcctgtgcct gcggggttga caaagccagt aagatccgcg catgcccttc
     3301 actgattcgc ccagcggtga gcgcctggcg cgcaacggcg gtcaggttaa gtaagcgcag
     3361 cgtattggtc accgttgagc gctttttacc cacctgcagg gctatctctt catgagaaag
     3421 gttgaactct tctgccagct gccggtaagc ctccgcagcc tccagtggat tcaggttcgc
     3481 gcgctgtaaa ttttcgatca gagccagctc taaatgctcc tgctcgctgg cttcacgcac
     3541 gataaccggc actttcctca ggccaatctg gcgagctgcc agcaagcggc gttccccggc
     3601 gatcaagatg tacgtccctg gctgatcgcc agagc
//
LOCUS       DATMXK010000322         3404 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118 k141_905,
            whole genome shotgun sequence.
ACCESSION   DATMXK010000322 DATMXK010000000
VERSION     DATMXK010000322.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3404)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3404)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3404
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_905"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..235
                     /locus_tag="VN363_05945"
     CDS             <1..235
                     /locus_tag="VN363_05945"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84088.1"
                     /translation="RLGGEGGPEVNLPGGDTPGETVVGAQERPAGDPGSLQVPYVDAL
                     PYYSEINQQAIDSGQVPLDLRELVRRYFSSLEP"
     gene            239..790
                     /locus_tag="VN363_05950"
     CDS             239..790
                     /locus_tag="VN363_05950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84089.1"
                     /translation="MITWLFQNRKSLQLGLLILLAASFLGPWTFEVDGVPPPEFCDSP
                     YVRLTPERCVRLVPGYEMVFTGFRFVVELFMGGLTASLNLREALNGIVIILVILVPLL
                     PLAAILWQVLHGDQELSRRGRAFFYASLVLGFIAALLPFVMNWPPRATLFWGLWAYLI
                     LNDSCLMLDILTQAGMRAQTAIR"
     gene            798..1775
                     /locus_tag="VN363_05955"
     CDS             798..1775
                     /locus_tag="VN363_05955"
                     /inference="COORDINATES: protein motif:HMM:NF040105.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWQ84090.1"
                     /translation="MTEITPDTFRQQAAAIEAAVGQVIVGQHSVVRHVLTAVLAGGHV
                     LLEGVPGLGKTMLVRTLGEVLRLKFARIQFTPDLMPADITGTEILSDQDGARRFRFQP
                     GPIFANLVLADEINRATPKTQSALLEAMQEGSVTVANQTYRLEEPFFVLATQNPLEME
                     GTYPLPEAQLDRFLFKVDVGFPSSAELVQIFERTTGAAGEPPAVAAGEEEILQLRALA
                     RQVPVASHLSEYVARLVVASHPQQPSAPELVRRYVRYGASPRAGQAILLGAKVSALLD
                     GRYNVSFEDIHAIAPAALRHRLLLSFEGLADEIPPDAIIAELLEKVEQL"
     gene            1849..2760
                     /locus_tag="VN363_05960"
     CDS             1849..2760
                     /locus_tag="VN363_05960"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWQ84091.1"
                     /translation="MLFDEATLRKLTRLTLIASQVRAGRMKGERRSSRKGASLEFADY
                     RDYSPGDDLRRLDWKAYARLDRAFLKLFEEEEDLAVHILLDGSQSMDWGEGEAHKFTY
                     ALRIAGALGAIALAEGDQLSVHLLTSTGSDPNGLLGPLRGAGNTLRLLRHLEGLQPGL
                     HPGGRPGGETDLAASLRAVAASRPRPGLVFLISDLLSPTSFAEGLAAFLRRGSEAVVI
                     QVLAPDELDPPLAGDLRLVDVETDLYQEVSLDSGARRLYRQRLGEWQAGLQAAARKYG
                     ARWTSLSTAEPWDQVVQASLWKAGVLQ"
     gene            2801..>3404
                     /locus_tag="VN363_05965"
     CDS             2801..>3404
                     /locus_tag="VN363_05965"
                     /inference="COORDINATES: protein motif:HMM:NF024909.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BatA and WFA domain-containing protein"
                     /protein_id="HWQ84092.1"
                     /translation="MNFLAPGWLWLGLLAIPILILYMLRLRRREVPVSSILLWKLLLR
                     EQQANAPWQKLERNLLLFLQLLALAALVLALARPALPTPSLVSGSVVVILDGSASMLA
                     ADAENGSPRFEAARVAALALVGDLPARARLSIVLAGDPPQIIAAAEDDPARLREALQN
                     AQAQPVEADWQATLALAAGAASAPGLSREAAEFILLSDGGL"
BASE COUNT          521 a         1161 c         1073 g          649 t
ORIGIN      
        1 gcggctgggt ggcgagggcg gcccggaggt gaacctgccg ggcggcgata cccccggtga
       61 gacggtggtc ggcgcccagg agcgccctgc cggagacccg ggcagcctgc aggtgcccta
      121 tgtggatgcg ctgccctact attccgagat caaccagcag gcgatcgaca gtggtcaggt
      181 cccgctcgac ctgcgcgagc tggtccggcg ctacttctct tccctggaac cataaaaaat
      241 gatcacctgg ctcttccaaa atcgaaaatc cctccagctc ggcctgctga tcctcctggc
      301 agcctccttc cttggcccgt ggacctttga agtggacggt gtcccgccgc cggagttctg
      361 tgattcgccc tatgtgcggc tcaccccgga gcgctgtgta cgcctggtgc cgggctatga
      421 gatggtcttt accggcttca ggtttgtagt cgaattattc atgggcggtc tcaccgccag
      481 cctcaacctg cgggaagcgc tgaatggaat tgtgatcatc ctggtcatcc tggttcccct
      541 gctgccgctg gctgccatcc tgtggcaggt gctgcacggc gaccaggagc tcagccggcg
      601 cgggcgggcc ttcttttacg ccagcctggt cctgggcttc atcgccgccc tgctgccttt
      661 cgtaatgaac tggccgccgc gcgccaccct gttctggggc ctgtgggcgt atctgatcct
      721 gaacgatagc tgcctgatgc tggatattct cacccaggcc gggatgcgcg ctcaaactgc
      781 catccgatag gagccctatg actgagatca ccccggatac ctttcgccag caagccgccg
      841 ccatcgaagc cgccgtcggt caggtcatcg tcgggcagca cagtgtggtc cgccatgtcc
      901 tgacggccgt gctcgccggc gggcatgtcc tgctggaagg tgtgccgggc ctggggaaga
      961 ccatgctggt gcgcaccctc ggcgaggtcc tgcgcctgaa atttgcccgc atccagttca
     1021 cccccgacct gatgccggcc gacatcaccg gcaccgagat cctgagcgac caggatgggg
     1081 cgcgccgctt ccgcttccag ccgggaccca ttttcgccaa cctggtcctg gcggatgaga
     1141 tcaaccgcgc cacccccaag acccaatccg ccctgctcga agccatgcag gagggctcgg
     1201 tgacggttgc caaccagact taccgcctgg aagagccctt cttcgtgctt gccactcaga
     1261 acccgctgga gatggaaggc acctaccccc tgcccgaagc ccagctggac cgcttcctgt
     1321 ttaaagtcga tgtcggtttt ccctcctcgg ccgagctggt ccagatcttt gagcgcacca
     1381 ccggcgcagc tggcgagccg cctgcggtcg cggctggaga ggaggagatc ctccagctgc
     1441 gcgccctggc gcgccaggtg ccggtagctt cccacctcag cgagtatgtc gcccggttgg
     1501 tggtcgcctc ccacccccag cagccttccg ctcccgagct ggtgcgcagg tacgtgcgct
     1561 atggagcttc cccgcgggcc gggcaggcca tcctgttagg cgccaaagtg agcgccctgc
     1621 tggatgggcg ctacaatgta tcttttgagg atattcacgc catcgccccg gctgcgctgc
     1681 gccaccgcct gctgctcagc tttgaaggcc tggcggatga gatccccccg gatgccatca
     1741 tcgccgagct gctcgagaag gtagagcagc tctagagaac ctgttaaacc ccagaagtat
     1801 aagaaatccg caggatttca acccctgggg tttaacaaga agaatccaat gctttttgat
     1861 gaagccaccc tgcgcaagct cacccgcctg acgctgatcg ccagccaggt ccgcgccggg
     1921 cgcatgaaag gcgagcgccg ctcgtcgcgc aagggcgcca gcctcgaatt tgccgattac
     1981 cgcgattatt ctcccggcga cgacctgcgc cgcctggatt ggaaggccta cgcccgcctt
     2041 gaccgcgcct tcctgaagct cttcgaagag gaagaagatc tggcggtcca tatcctcctg
     2101 gatggctcgc agtcgatgga ttggggagag ggggaggcgc ataaattcac ttacgctctg
     2161 cgcattgccg gcgcgctggg ggcgattgcc ctcgccgaag gcgaccagct cagtgtgcac
     2221 ctgctgacca gcaccggctc ggatcccaat gggctgctcg ggccgctgcg cggggctggc
     2281 aataccctgc gcctgctgcg ccatctcgag ggtctccagc ccggcctgca ccccggcggc
     2341 cgtcctggag gcgagactga ccttgcggct tccctgcgcg ccgtggctgc cagccgcccc
     2401 cggcccgggc tggttttcct gatctccgat ctgctctcgc cgaccagctt cgccgaaggc
     2461 ctggcggctt tcctgcgccg cggctcggag gcggtggtga tccaggttct ggcgcccgat
     2521 gagctcgacc cgcccctggc aggcgacctg cgcctggtgg atgtcgagac cgacctgtac
     2581 caggaggtct cgctcgattc cggggcgcgc cgcctgtacc gccagcgcct gggtgagtgg
     2641 caggctggcc tgcaggctgc cgcacgcaaa tacggggcgc gctggaccag cctgtccacc
     2701 gccgagccct gggaccaggt cgtgcaggcc tcgctctgga aagcaggcgt actgcagtga
     2761 tgcctgattc tctggccgga tatccgaagg gcgtgctcca gtgaatttcc ttgccccggg
     2821 ctggctgtgg ctgggactgc tcgccatccc cattctgatc ctgtatatgc tgcgcctcag
     2881 gcggcgggaa gtaccggtct cgtcgatcct gctatggaaa ttactgctgc gtgagcagca
     2941 ggccaatgcg ccctggcaaa agctggagcg caacctgctc ctgttcctgc agctgctggc
     3001 tttagccgcc ctggttctgg cgctggcgcg gccggccctg cccaccccca gcctggtcag
     3061 cggttcggtt gtggttatcc tggatggctc ggcttccatg ctggccgcag acgcggagaa
     3121 cggcagcccg cgcttcgagg cagcccgcgt tgccgccctg gcgctggttg gcgacctgcc
     3181 tgccagggcg cgcctgagca ttgtcctggc cggtgacccg ccccagatta tcgccgccgc
     3241 cgaggacgat cccgcccggc ttagagaagc cctgcagaat gcacaggctc agccggtgga
     3301 agccgattgg caggcgactc tggcgctggc tgccggggca gcctccgcac ccggcctttc
     3361 ccgcgaggcg gccgaattta tcctgctttc ggatggcggc ctgg
//
LOCUS       DATMXK010000323         5624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_152288, whole genome shotgun sequence.
ACCESSION   DATMXK010000323 DATMXK010000000
VERSION     DATMXK010000323.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5624
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152288"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            131..>5624
                     /locus_tag="VN363_05970"
     CDS             131..>5624
                     /locus_tag="VN363_05970"
                     /inference="COORDINATES: protein motif:HMM:NF013672.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWQ84093.1"
                     /translation="MKTKRLLLNLLPLIALLLNPFAPLAVAMEADAPTLRVKADLAGY
                     AGHIQLSVTNQASTQIPNRYTLQYILDTQSLISAGELQADCDDLRVTYVDGVNEFELD
                     RVVEGCNTPTTTLSFRNREIVAIGGTDLGYHVYYNNPGAGAPPASPFNVYTFYDDFQD
                     GSAAEWIAADGTWGVFDDGGNFVYRYTGGGTNWALAYTPVSGVNNLELFSQVRAAADT
                     NWIGLAFRIQDPANFLTFYQSKDTSQFKYARIAADDHAVLQSPAFTMAADAWYNIRVR
                     AVSSLVQARIWLASDPEPAVWTIQFTDATYQTRRDIGATLYQHVTNADWDNFQVRKLV
                     DVEPLVTVYVPPTPIADWYYRAPITVTNSSASATLPVRYTVAITLDTASLIAAGKMLA
                     TCEDLQIASLVNTDMFEIDRVVENCNTAATRVWFALQRPILPGASDAGYYIIYGDPSP
                     APPKAEEMNVFLFFEDWEQSATHWTNAGGLDPANTGTLGLSIISTEAAVSPTNSHKFP
                     FKVGGGDAFSGFIPVNANTTYAIATWAKSGESTYAPVGFDPYTAAYAKGVETWLWTSD
                     WTISPDWTWRSGSFTTAATTAFIKIKSEWWAEAPGNQPVYMDNLGLRYALADEPGLAL
                     GDEETTLAVPVITNIVNNGPVDLGAPITISADIATTEGEVDYAQIRLISPVSLDIPLS
                     LVSGTPTGGLWEGSYLAAQGGEFSYRILAHATTLRQALSPLQTFTVLDTLPPVLTNLT
                     FTDPILVRETQTVSLEVTDNGAISSVILAVGGAAYPMTVSGGVYSYSWEVTTIGETPF
                     TVTATDSAGNPTALADSFTVQPRAVDVCTWKDCKAGAESFSIDDSNQSCKANLETAGF
                     RGTYFVNGSTTQAWYTTYSTDGHEIAAHTVTHPCNPPACFPNCTPESLLQIPIDPAVV
                     TAYRLNELEPNIAAIEAGTGKPVLALAWPCGCTDPSRMAAASAYLLGARGYFDSIANL
                     TWLQDVNLPDPVNFFNLNAGNAYSQQLIDQAAAEGKWAIITSHGDCSGITYMGSRQDV
                     LWAAPVGEVLEYIYIRQNAVFSNYSRLVSTISFDAVHTLPPFLRTKLDDTNFLPLGFT
                     SPVSLKVHILDTDTVLSVEVDGVPVPYVLKSLEGAQYVVFDSPLNTSKHIAVNLGAPR
                     PTIVSVTDNSPVELGLEAQISAVVTLPAGTLTSVTLQVINPAAADYAMLPVEGSPDTY
                     AASFTPPGLGDYTYRVLATNDSGGSTLSGEYTLTVVDTTPPAWRNLAQTAPSILVGEA
                     NTLSAEGLDIGGLGWATLETNETGVWQAITWPVSNWWDHNWTKRVPVTFNETAGLARS
                     AETVDILITAAQFPGLTSCAAELRVADADRNELPGQVYGETGSGDTLACHLLFQASLA
                     ANASRTYFIYFGNPAAAAPGYTTDLSSTPSPGIINLQNSFFNLDLDVDSGVISRLRLP
                     QGSDANLPLSSEADVYWGWHQVCSSLHGNITGKNNLCVGGTAPASGLSLATTLDGPIV
                     KEYTLTSVKTGTTYTIVYRFFANTPHYQYRLTQTGTPASVMNNFWYTNGFFPRLGTGS
                     GGTPATTYNLYDYTTDQARIASFTTVNVAAIDGLDNDGTDLGGSDYMHPTAPGLELWV
                     TTAASQAAAETELGRVAAPLGSLLGGVEDAPEAVYGSPLNLNGAVVWTPAGFVWQNPA
                     IPVGTDVSWRITFCDLADNCVTTSEMTFAIVPPNTAPIANAQAVSTNEDTPLAITLTG
                     SDLELDPLTFTVVDNPVHGTLSGTAPDLIYTPAADFNGDDNFTFTVSDGEFVSAPAAV
                     SITVEPVNDAPVAAAQAVSTDEDTQLAITLTGSDVDLDPLTFAVVDN"
BASE COUNT         1169 a         1789 c         1600 g         1066 t
ORIGIN      
        1 aaccagaggt gtctgatcca gctcgaaacc ggaaaacaaa accaaggtac atttgtgggg
       61 atctacagga aaatggggca cgccggggcg ggattccacg cgcgctgctc cacatcacaa
      121 ggagaaccaa atgaaaacta aacggctttt gcttaacctg cttccgctga tcgctttgct
      181 attaaatccc tttgcacctc tggcagtggc gatggaggcg gatgccccaa cactacgcgt
      241 caaggctgat ctggctggat atgcagggca tatccagctg agcgtgacca accaggcgtc
      301 gacccagatc ccgaaccgct atacactgca gtatattctg gatacccaga gtctgatcag
      361 tgccggtgag ctgcaggccg attgtgatga cctgcgcgtt acctatgtcg acggcgttaa
      421 cgagttcgag ctggatagag tcgtcgaagg ctgcaatacc cccaccacga cgctcagctt
      481 ccgcaaccgg gagatcgtcg ccatcggggg aaccgacctg ggctaccacg tctactacaa
      541 caaccccggg gcaggcgctc cgccggcctc accctttaat gtctacacct tttatgatga
      601 tttccaggat ggcagtgccg cggagtggat cgctgctgat ggcacctggg gtgtattcga
      661 tgacggcggc aatttcgtct atcgctatac tggcgggggg acgaactggg ccctggcgta
      721 cacaccggtc agcggcgtca acaacctgga gctgttcagc caggtgcgcg ccgcagccga
      781 caccaactgg atcggcctgg catttcgcat ccaggatccg gccaatttcc tcacttttta
      841 ccagagtaaa gataccagtc aatttaaata tgcccgcata gcagccgatg accacgcggt
      901 gctgcaatca ccggccttca ccatggcagc agatgcctgg tataacatcc gggttcgagc
      961 tgttagctca cttgtacagg cgcggatctg gctggccagc gatccggagc ctgccgtttg
     1021 gacaatccag tttaccgatg cgacctacca gacccgcagg gatatcgggg caaccctgta
     1081 ccagcatgtg accaacgccg attgggataa tttccaggtg cgcaagctgg ttgatgtcga
     1141 gccgctggtg acggtttatg tgccacccac accgatcgca gactggtatt accgcgcccc
     1201 gattacagtc accaattctt cagcgtcggc gaccctgccc gtgcgttaca cggtcgcgat
     1261 cacgctcgat acggccagcc tgatcgcggc tggcaaaatg ctggcgacct gtgaagacct
     1321 tcagatcgcc tccctggtaa acacagatat gtttgaaatt gatcgggttg tcgaaaactg
     1381 caacaccgcg gcaacccgag tctggttcgc tctccagcgg ccaatcctgc ccggggcaag
     1441 cgatgcgggc tattacatca tctatggcga cccgtccccg gctccgccca aagcagaaga
     1501 gatgaatgtc ttcctgtttt ttgaggactg ggaacaatca gccacccact ggacgaatgc
     1561 cggcggattg gacccggcga acacaggcac gctggggctg tcaatcatct ctaccgaggc
     1621 ggcggtctca cccaccaaca gccataagtt cccattcaag gttggcggcg gagatgcttt
     1681 ctcggggttt atcccggtga acgccaacac cacctacgcc atcgctacct gggcgaaatc
     1741 aggtgagagc acttacgcgc cggtcggctt cgacccctac acagcagcct acgcgaaagg
     1801 cgtcgaaacc tggctgtgga ccagcgattg gacgatatcg cccgattgga cctggcgcag
     1861 cggcagcttt accaccgccg cgaccacagc cttcatcaaa atcaagagtg aatggtgggc
     1921 agaagccccg ggcaaccagc cagtctatat ggacaatctg ggcctgcgct atgcccttgc
     1981 cgatgagccg ggcctggccc tgggggatga agaaaccacc ctggcagtcc cggtgatcac
     2041 caatatcgtc aataatggac cggtggatct gggcgctcca attacaattt cggcggatat
     2101 cgccaccacg gagggcgaag tcgactacgc ccagatccgg ctcatctcgc cggtgagcct
     2161 ggatatcccg ctgagcctgg tctccggcac gccgaccggc gggctctggg agggcagcta
     2221 cctggctgcc cagggcggtg aattcagcta ccgcatcctg gctcacgcca ccaccctgcg
     2281 ccaggcgctt tcgccgctgc agacctttac ggtcctggat accctgccac ctgtgctcac
     2341 caatctcacc ttcaccgatc cgatcctggt gcgggaaact cagacggtga gcctggaggt
     2401 gacggataac ggggcgatca gttcggtaat tctggccgtt ggcggagcag cttaccctat
     2461 gaccgtgagc ggtggggtat acagctactc ctgggaggtg accacgatcg gagagactcc
     2521 cttcactgtg accgctaccg acagcgccgg caatcccacc gcgctggcag acagcttcac
     2581 cgtccagccg cgcgcagtgg atgtatgcac gtggaaggac tgtaaagccg gtgcggagag
     2641 cttctcaatc gatgacagca accaatcctg taaagccaac ctggaaacag ccggttttcg
     2701 cgggacgtat tttgtgaacg gcagcacgac ccaggcctgg tacacgacct actccaccga
     2761 tggccacgaa atcgcggctc acactgtaac ccatccctgc aacccgccgg cttgcttccc
     2821 caactgcacc ccggaaagcc tgctgcagat ccccatcgat ccggctgtgg tgacagctta
     2881 ccggctgaat gagctcgagc cgaatatcgc cgccattgag gccggcaccg gcaaaccggt
     2941 gctcgctctg gcctggccct gtggctgcac cgacccgagc aggatggccg cagcttctgc
     3001 ctacctgctg ggggcgcgcg gctatttcga ctcgatcgcc aacctcacct ggctccagga
     3061 tgtcaatctc cccgatccgg ttaacttctt taacctgaat gccggcaatg cttacagcca
     3121 gcagctgatc gaccaggcgg cggctgaggg caaatgggcg attatcacct cccacggcga
     3181 ctgcagcggg atcacttata tgggctcacg ccaggatgtc ctgtgggcag ctccggtggg
     3241 cgaggttctg gaatacatct atatccgcca gaacgctgta ttcagtaatt actcccgcct
     3301 ggtaagcacg atctcgttcg atgccgtcca taccctcccc ccattcctgc gcacgaagct
     3361 ggatgataca aacttcctgc ccctgggatt taccagcccg gtcagtctca aagtccatat
     3421 cctggacacg gacaccgttt tgagcgtgga agtggatggc gtgccggttc cgtatgtgct
     3481 caaatcgctg gagggcgccc agtacgtggt gtttgattcg cccttgaata ccagtaagca
     3541 cattgccgtc aacctgggtg ctccacgacc gacgattgtc tcggtgacgg acaacagccc
     3601 ggtggagctg ggcctggagg ctcagatttc cgccgtggtt acactcccgg ccggcacgct
     3661 gaccagcgta accttacagg tcatcaaccc tgcggctgca gattacgcca tgctccccgt
     3721 ggaaggctcc cccgatactt acgccgccag tttcaccccg ccaggcctgg gcgactatac
     3781 ctaccgggtg ctggcgacca acgacagcgg tggcagcacc ctgtccgggg aatacacgct
     3841 gacggtcgtg gatacgaccc caccggcgtg gcgcaacctg gcgcaaactg cgcccagcat
     3901 cctggttggc gaagccaaca ccctatccgc cgaagggctg gacatcggcg ggctgggttg
     3961 ggcaaccctg gagaccaatg agaccggtgt ctggcaggca attacctggc cggtgagcaa
     4021 ctggtgggat cataactgga caaagcgcgt gccggttaca tttaatgaga ctgcaggact
     4081 ggcgcgcagc gctgaaacag tcgatatttt aatcaccgca gcccagttcc caggcctgac
     4141 ctcctgtgcg gctgagctgc gggtggcgga tgcggatcgc aatgagctgc ccggccaggt
     4201 gtacggtgaa accggcagcg gagataccct ggcctgccac ctgctcttcc aggccagcct
     4261 ggccgcgaat gccagccgca cgtacttcat ctacttcggg aatccagcgg cggcagcgcc
     4321 cggctacacc accgacctga gcagtacgcc cagcccgggg atcatcaacc tgcagaacag
     4381 cttcttcaac ctcgacctgg atgtggacag cggggtgatc tcacgcctgc gcctgccgca
     4441 gggcagtgat gctaacctgc cgctctcgtc tgaggcggat gtgtactggg gctggcacca
     4501 ggtctgctca tccctgcacg gcaatatcac cggtaaaaac aatctatgcg tgggcggcac
     4561 agccccggcg agtggcctga gcctggcaac taccctggat ggcccgatcg tcaaggaata
     4621 caccctgacc agcgtgaaaa ccggtaccac atacaccatc gtctaccgct tcttcgccaa
     4681 tacgccccac taccagtacc ggctgactca aaccggaacg cctgccagcg tgatgaacaa
     4741 cttctggtac acgaatggct tcttcccccg cctgggcacc ggcagcggcg ggacacccgc
     4801 cacaacctat aacctgtacg actacacgac cgaccaggcc cggatcgcgt ccttcaccac
     4861 ggttaatgtc gccgcgatcg acgggctgga caatgatggc accgacctgg gcggcagcga
     4921 ctacatgcac ccgacagccc ccgggctgga actgtgggtg acgaccgcag ccagccaggc
     4981 tgcagcggag accgagctgg gtcgcgtggc tgcgccactg gggtcgctcc tggggggtgt
     5041 cgaagatgct cctgaagccg tctacggctc accgctcaat ttgaatgggg cggtggtgtg
     5101 gactccggcg ggctttgtct ggcaaaatcc agccatcccg gtggggaccg acgtaagctg
     5161 gcggattaca ttctgcgatc tggcggacaa ctgtgtcaca acctctgaaa tgacattcgc
     5221 gatcgttccg cccaacacag cccctatcgc gaatgcccag gcagtgagca caaatgaaga
     5281 tactccgctg gcgattaccc ttaccggctc agacctggag ctcgatcccc tgaccttcac
     5341 agtggtggat aacccggtgc atgggactct cagcggcacc gcgccggacc tgatctatac
     5401 cccggctgca gacttcaacg gcgacgacaa cttcaccttc actgtcagcg acggggagtt
     5461 cgtctcggcc ccggcggcgg tgagcatcac cgtcgagccg gtcaatgacg ccccggtggc
     5521 ggctgctcag gcagtttcaa ccgacgagga tacccagctg gccattaccc tgaccggctc
     5581 ggatgtcgat ctcgacccgc tgacctttgc cgtggtggat aacc
//
LOCUS       DATMXK010000324         3013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_103304, whole genome shotgun sequence.
ACCESSION   DATMXK010000324 DATMXK010000000
VERSION     DATMXK010000324.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103304"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(33..293)
                     /locus_tag="VN363_05975"
     CDS             complement(33..293)
                     /locus_tag="VN363_05975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84094.1"
                     /translation="MKTGFVPISIEEYVELHIKDNPDANREEITTVLNDALDAYQRGA
                     KCTNCENPIWVIGSAFAGNMCFTCITGEAIPEDDYEIDEACY"
     gene            962..2875
                     /locus_tag="VN363_05980"
     CDS             962..2875
                     /locus_tag="VN363_05980"
                     /inference="COORDINATES: protein motif:HMM:NF039653.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6094 domain-containing protein"
                     /protein_id="HWQ84095.1"
                     /translation="MRLAGIEKGGYYPYPPHMVEATASWFIPLPEGSRGRLLDPCAGE
                     GEIANLMGRLLNCETWGCELFPYRAEKAAAHMDKCHNAAWESCSLTDESVTLLWLNPP
                     YDDDRHGDEKRLELAFLKSTMPKLVRGGVLAFVIPQRILRLVEIARYMTSQYERLTVL
                     RFPDGEYERFKQILLLATRRDKYNVPTTEEIEAIQRLAESDLPPLGFAPEPVYPLLPA
                     PLKGTGGRVITFRRLDWAPDDLVEASRWAGVRKTRAWQDLVHPVNSEVAFTPTMPLKK
                     GHLAMLMASGMMGTLHLTSDDGYPMLVKGRVVKVVDKVVHRDPEDPETVVERYRDRYV
                     TTVATLSQHGLELIQDVDSLTRFMRQYGDKIAAHTLETYQPLYDLKPTEVELAVLESL
                     GKTRKALPGQVEAGLLPTQKHAAIAAARAIRQHRTANLQGEMGLGKTTIALAVIDLLD
                     AYPALILCPPHLVPKWIREAEEVIPGAKGRELRRIGKGEDGEINDVRQFFDDFDVGRL
                     GPKAIAVVASTSAKMGAGWQPVVRAKPIKVKGKAQVIYTCPQCGQVQIDPQGIPITEI
                     EIFQKHRSFCNAQVSGWELDRDGRRKLDKDGNPNWGTRPCGAPLFEYTGVRRWSIAEY
                     VKEKDNGRFQILI"
     gene            complement(2888..>3013)
                     /locus_tag="VN363_05985"
     CDS             complement(2888..>3013)
                     /locus_tag="VN363_05985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_076612403.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ISAs1 family transposase"
                     /protein_id="HWQ84096.1"
                     /translation="AILRHMALNMLKQERSAKLGIQAKRLQAGWDEDYLLKVLSV"
BASE COUNT          716 a          839 c          802 g          656 t
ORIGIN      
        1 ggtcaaacat agactcatcc agattgaaat catcagtagc aagcctcatc gatttcataa
       61 tcgtcttctg gaatagcttc accagtaatg caggtgaagc acatgttacc ggcaaaagct
      121 gatccgatta cccagatagg attttcacaa ttagtgcatt ttgcgcctct ctggtaagca
      181 tccaatgcat cattaagcac ggtggtaatt tcctccctgt ttgcatccgg gttgtctttg
      241 atatgaagtt ctacgtactc ttcgatgcta atcggtacaa agccagtttt cattatcgaa
      301 gttacctttc tcgctattgc taatcaattt gtgaatcaac cagttggtaa ggtcacgaaa
      361 tcaacccagg accatgtaaa aggctaattc gctgtaaacc gttgtaagcc aatgtttact
      421 taggcggtgc tactaatgac tgcacggcgt tgcaaatggg ttaccaaaat ggtagatatt
      481 tgcccaggag taaaccggcc aataggtaat gtcgaatata gcacctaaag catttccaaa
      541 cacctcgggt tccacgaatt cgccttgagc cattcgtgct tctctacagg cgcctaacgc
      601 gctattccac ttgatgtgaa acccgaaacc aagtccgaga tagataagca cgaaagccaa
      661 gagaatgtaa acgatcttgc gaatgttgat attctgcgtc atcatttctt gcgctctcat
      721 taatccgact aaaagttgat tccttggaat tatttccacg caaatctgat tctaatgcgc
      781 ggaattcaaa acgttggcag gaacagatgt taagaacaaa aaaccaggag gtgaaactta
      841 acaacatttt agattaggac tctcattcac acaagtgttc aaaatccttt tcccccgcgg
      901 ggacatcgcg cccctcaggg gtaacatgtc ctccgggatc attcacacgg aggatacaga
      961 catgagactt gcaggcattg aaaaaggcgg atactaccct tatccacctc atatggtgga
     1021 agctacggct tcctggttca tcccccttcc agaaggctca cgcgggcgtc tccttgatcc
     1081 gtgcgctggc gaaggcgaaa ttgccaacct gatgggccgg ctactcaact gcgagacctg
     1141 gggctgcgag ctgttccctt accgcgccga gaaagccgct gcccatatgg acaagtgcca
     1201 caacgccgcc tgggagagct gctctctgac tgacgagtcg gtcacgctct tatggctcaa
     1261 cccgccgtat gatgacgatc ggcacggtga tgaaaagcgg cttgagctgg ctttcctgaa
     1321 atcgaccatg cccaagctgg tgcgcggcgg cgttctggcg ttcgtcattc cgcagcgcat
     1381 cctgcgactg gtggagatcg cccgctacat gaccagccaa tatgagcgcc tcaccgtcct
     1441 gcgttttcca gacggcgagt acgagcgctt caagcaaata ttgcttctgg cgacacggcg
     1501 agataagtac aacgtcccca ctaccgaaga gatcgaagcc attcagcgct tagcggagag
     1561 cgatctgccg ccgttaggat ttgcccctga accggtctac ccgctgttgc ccgccccgct
     1621 gaagggcacg ggcggcaggg taatcacctt tcgccggctg gattgggcgc ccgacgacct
     1681 ggtggaggcc agccgctggg cgggtgtgcg caagacccgc gcctggcagg atctggttca
     1741 tccggtcaac agcgaggtcg ctttcacccc caccatgccg ctcaagaaag gccacctggc
     1801 gatgctgatg gcctctggca tgatgggcac tctgcacctg accagcgatg atggctaccc
     1861 aatgctggtc aaaggacggg tggtcaaggt ggtggataaa gtcgtccacc gtgatcccga
     1921 agacccggag acagtcgtcg agcgttaccg cgatcgttac gtcacgaccg tcgccacgct
     1981 ctcacaacac gggctggagc tgatccagga tgtggactcg ctgacgcgtt tcatgcgcca
     2041 gtacggcgac aagatcgccg cccacaccct ggaaacttac cagccgttgt acgatctgaa
     2101 acccacggaa gtagaactgg ctgttttgga aagcctgggt aaaacccgca aggcgctgcc
     2161 cggtcaggtt gaagccggtc tgcttcccac gcagaagcac gccgcaattg ccgccgcccg
     2221 cgccatccgc caacaccgaa ctgccaatct gcagggcgag atgggattgg gcaagacgac
     2281 cattgccctg gcagtgatcg acctgctgga cgcttacccg gcgctgatct tgtgcccgcc
     2341 gcatctggtt cctaagtgga tccgggaggc ggaagaagtc atccctggag ccaaagggcg
     2401 cgagctgcgc cgcatcggca agggtgaaga tggcgagatc aacgatgttc gccagttctt
     2461 cgatgacttt gacgttggca ggttgggtcc caaggcaatc gccgtggtgg cttccacctc
     2521 ggccaagatg ggcgctggtt ggcagccggt ggtcagagca aaaccgatca aggtaaaagg
     2581 caaagcccag gtcatctata cctgcccgca gtgtggacag gtgcagatcg atccccaggg
     2641 catcccgatc accgagatcg agatcttcca gaaacaccgc agcttctgca atgcgcaagt
     2701 atctggctgg gagctggacc gagatggtcg ccgtaagctg gacaaggatg gcaaccccaa
     2761 ctggggtaca aggccttgtg gagcaccgtt gttcgaatac accggcgtgc gtcgttggtc
     2821 tattgccgag tacgtcaagg agaaagacaa cgggcgcttc cagatcctca tctagggtaa
     2881 tcgcatttca aaccgagagc accttgagca agtaatcttc gtcccagcca gcttgtaggc
     2941 gcttagcctg gatgcccaat ttggccgacc tttcctgctt cagcatgttg agcgccatat
     3001 gacgcaatat tgc
//
LOCUS       DATMXK010000325         5658 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_108961, whole genome shotgun sequence.
ACCESSION   DATMXK010000325 DATMXK010000000
VERSION     DATMXK010000325.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5658)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5658)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5658
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108961"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..941
                     /gene="proB"
                     /locus_tag="VN363_05990"
     CDS             <1..941
                     /gene="proB"
                     /locus_tag="VN363_05990"
                     /EC_number="2.7.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559641.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004349 - glutamate 5-kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glutamate 5-kinase"
                     /protein_id="HWQ84097.1"
                     /translation="YEQLFGLYGITVAQVLLTRADLADRRRYLNSRSTLAALLDQRVI
                     PIINENDTVATEEIRFGDNDNLSALVTNLTEADLLALLTDQPGLFTADPRRDPGARLV
                     QEVNGSEIPEALWQAAGGTIGGLGTGGMFTKLQAASLARRGGAAVVIASGQEPDILLR
                     LAAGEGTGTHFHPVASALESRKRFILAGGPTTGEVWLDAGAERALRRGGSLLPVGVTR
                     VSGAFERGDTVRLVDPQGREIARGLANYSAEDLARIAGQHSDKIESLLGYAYAAEVIH
                     RNNLVLLAPHAVAGDEASYTALPVSDSADEIEETGR"
     gene            938..2206
                     /locus_tag="VN363_05995"
     CDS             938..2206
                     /locus_tag="VN363_05995"
                     /EC_number="1.2.1.41"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559642.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004350 - glutamate-5-semialdehyde
                     dehydrogenase activity [Evidence IEA];
                     GO_function: GO:0016620 - oxidoreductase activity, acting
                     on the aldehyde or oxo group of donors, NAD or NADP as
                     acceptor [Evidence IEA];
                     GO_process: GO:0006561 - proline biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate-5-semialdehyde dehydrogenase"
                     /protein_id="HWQ84098.1"
                     /translation="MMNEMGKRARQAASWLASAASAQKDAALECMADTLLARQDAILE
                     ANQADLAAGERSGLSAALLDRLMLNPARLAGIVGDLRQLVRLPDPVGEIFEAGVLPNG
                     LRIRKQRVPLGVLGVIYEARPNVTVDVAGLAIKTGNAAILRGGSETMNSNRELVSALR
                     QALEDSGMPADVIQWVSDPDRARVLELLHLDESVDMIIPRGGAGLHDFCRRNSHIPVI
                     TGGIGICHLFVDASADVEKALPVIFNAKVQRPSVCNSLDTVLVHQSVAAEVLPKVVAH
                     LRPAGVSFRAEPRAQASLQPLTAGEDSASVLPAGPEDFDTEWLSLVLGLKVVDDLEEA
                     VAHIRQHSTSHSDGILTEDSTNARRFVEQVDSAVVYVNASTRFTDGAQLGLGAEVAVS
                     TQRLHARGPMGLRELTTYKWVVEGDYHVRG"
     gene            complement(2257..2847)
                     /locus_tag="VN363_06000"
     CDS             complement(2257..2847)
                     /locus_tag="VN363_06000"
                     /inference="COORDINATES: protein motif:HMM:NF012314.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thioredoxin domain-containing protein"
                     /protein_id="HWQ84099.1"
                     /translation="MGNELNNPPGSRQASLLASLQAHPLPVIVEFWAAWCAPCRAMES
                     GLLRTGEEFKGKVDLWRINADEHPEILHALKVYGIPTMILFQNGQELARRAGAQSPAD
                     LMAFFRAAEAGEAPPARSLTPVERFLRAGSGLAGLFVAYQGGFEGFSLALAIASGVLI
                     FSAVYDRCPIWKAVSSRVREWLVGSESEAGQPQKSS"
     gene            complement(2849..3049)
                     /locus_tag="VN363_06005"
     CDS             complement(2849..3049)
                     /locus_tag="VN363_06005"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558733.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF2892 domain-containing protein"
                     /protein_id="HWQ84100.1"
                     /translation="MKVNQANWERSVRVLLGVALLYAGFGRMVTGTWGIVAEIVGALA
                     LLTGLSGWCPIYALLKFSTKKA"
     gene            complement(3243..>5658)
                     /gene="clpB"
                     /locus_tag="VN363_06010"
     CDS             complement(3243..>5658)
                     /gene="clpB"
                     /locus_tag="VN363_06010"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ATP-dependent chaperone ClpB"
                     /protein_id="HWQ84101.1"
                     /translation="ALRDELTLELETRSRVYGASGDIGLAPPTAKALEAAERYAKGMQ
                     DDYVSAEHLLLGLTESSEGTRLGQYGLTKDAILKALTGIRGTQRVTSATPESTYQSLE
                     KYGRDLTAQARQGKMDPVIGRDEEIRRIVQILSRRTKNNPALIGDPGVGKTAIVEGLA
                     QRMVNGDVPEGLKHKRLVQLDMGALVAGAKYRGEFEERLKAVLKEISDAAGEIILFID
                     EMHTVVGAGAAEGAMDAGNMLKPMLARGELHLIGATTLDEYRKHVEKDPALERRFQPV
                     LVEEPSVEETISILRGLKERYEVHHGVRITDPAVIAAATLSNRYITDRHLPDKAIDLI
                     DEAAARLRTEIDSKPQALDEVDRQIMQLEIERQALKKEKDKASRERLEKLEKEVADLR
                     ERATELHTRWKTEKEAITSLQAVKEQIEQTRTEIERAERQADLEKAARLRYGSLRELE
                     SKLAEGELRIKALQKEGALLKEEVDAEEVAQIVGRWTGIPVSRLLEGETEKLIHMEER
                     LRARVVGQDDALRVVSNAVRRARAGLQDPNRPIGSFIFLGPTGVGKTELARALAEFLF
                     DDERAMIRIDMSEYQEKHTVSRLVGAPPGYIGYEEGGQLTEAVRRRPYSVVLFDEIEK
                     AHPEVFNVLLQLLDDGRLTDGQGRTVDFRNTVVIMTSNLGNQLWEGGRTAGREELTRT
                     LQAHFRPEFLNRIDEIVVFHSLGREQLAQIVEIQLRRSVQLVAERGFILDVSPAAREY
                     LAEVGYDPDFGARPLKRAIQRELQDPLALKLLAGEFQEGDTIFVDRGSEGLTFKAQII
                     PEVVEE"
BASE COUNT          993 a         1927 c         1547 g         1191 t
ORIGIN      
        1 tctacgagca gcttttcggg ctgtacggca taaccgttgc ccaggtcctg ctcacccgtg
       61 ccgacctggc cgaccgccgc cgctacctta attcgcgcag caccctggca gccctgctcg
      121 accagcgtgt tatcccgatt atcaatgaaa acgacaccgt cgccaccgaa gagatccgct
      181 tcggggataa tgataacctc tccgccctgg ttaccaatct gaccgaagcc gacctgctcg
      241 ctctgctcac cgatcagccc ggcttgttca ccgccgaccc gcgccgcgat cccggggccc
      301 gcctggtgca ggaagtcaat ggcagcgaga tccccgaagc tctctggcag gcagccggcg
      361 gcactattgg cggtctcggc accggcggca tgtttaccaa gctgcaggct gccagcctgg
      421 cgcgccgcgg cggtgcggca gtcgttatcg ccagcggtca agagccggat atcctgctcc
      481 gcctggctgc cggtgaaggt accggcactc acttccaccc cgtcgccagt gccctggaga
      541 gccgcaagcg ttttatcctg gcaggcgggc ctactaccgg tgaagtctgg ctggacgcgg
      601 gagccgagcg agccctgcgc cgcggcggca gcctgctgcc tgtcggcgtc acccgggtca
      661 gcggcgcttt cgagcgcggc gataccgtcc gcctggtcga cccccagggt cgtgagatcg
      721 cccgaggtct tgccaattac agtgccgaag acctggcgcg cattgccggc cagcattcgg
      781 ataagatcga aagcctgctg ggttatgcct atgctgctga agtcatccac cgcaacaatc
      841 tggtcctgct cgccccgcat gcggttgccg gcgacgaggc cagttatacc gctttaccag
      901 tttcagattc tgcggatgaa attgaggaga caggccgatg atgaacgaaa tgggaaaacg
      961 cgctcgccag gctgccagtt ggctggcctc cgccgcgagc gctcaaaaag acgccgctct
     1021 ggaatgtatg gccgatacgc tcctggcccg ccaggatgct atcctggaag ccaaccaggc
     1081 cgacctggct gccggggagc gcagtgggct gtcagctgcc ttgcttgatc gcctgatgct
     1141 caaccccgcc cgcctggcag gtatcgtggg cgacctgcgc cagctcgtcc gcctgcccga
     1201 cccggtgggc gaaatctttg aagccggtgt cctgcccaac ggcctgcgca tccgcaagca
     1261 gcgcgttccc ctgggcgtgc tgggcgtgat ttatgaagcc cggcccaatg tcaccgtgga
     1321 tgtcgccggt ctggccatca aaactggcaa cgccgccatc ttgcgcggcg gcagtgaaac
     1381 catgaattcc aaccgcgagc tggtcagcgc cttacgccag gcgctggaag acagcggtat
     1441 gccggctgat gtcatccagt gggtgagcga ccccgaccgc gcccgcgtcc tcgagctgct
     1501 ccacctcgat gaaagcgtgg acatgattat tccgcgcggc ggcgccgggc tgcacgattt
     1561 ctgccgccgc aacagccata tcccggtcat caccggcggg atcggcatct gccatctctt
     1621 cgtcgacgcc agcgcagatg tggaaaaggc cctcccggtg atcttcaatg ccaaagtcca
     1681 gcgcccctcg gtctgcaatt ccctcgacac cgtccttgtc caccagagtg tggcggcaga
     1741 ggtcctgccc aaagtggtcg cccacctgcg ccctgccggg gtaagcttcc gcgccgagcc
     1801 gcgcgcccag gccagcttgc agcctttaac cgcgggggaa gattccgcca gtgtgctgcc
     1861 tgccggaccg gaggattttg atacggaatg gttatctctg gtattggggt taaaagtggt
     1921 tgatgatctg gaggaggctg tggcccatat tcgccagcat tccacatccc actccgacgg
     1981 catcctgact gaagattcta ccaatgcccg ccgttttgtg gagcaggtcg attctgctgt
     2041 ggtctacgtc aacgccagca ctcgctttac cgatggcgcg cagctgggac tgggtgccga
     2101 agtcgcagtc tctacccagc gcttacatgc ccgcggcccg atggggctgc gcgagctgac
     2161 cacttacaaa tgggttgttg aaggcgatta tcacgtccgg gggtaattga cagaaacaga
     2221 attttccgag atttaaaaaa tcttgtagat cagcctttat gaactttttt ggggctggcc
     2281 cgcctcggat tctgagccta ccagccattc ccgcacgcgg ctgctcaccg ctttccagat
     2341 cgggcagcgg tcatacactg cgctgaatat cagcaccccg ctggcaatcg ccagcgccag
     2401 ggaaaatccc tcgaatccac cctggtatgc cacgaacagg ccggccagac cggagccggc
     2461 gcgtaagaag cgttctaccg gcgtcagcga gcgcgccggg ggcgcttctc cagcctctgc
     2521 tgccctgaaa aatgccatta aatcggccgg cgactgggct cccgcccgcc gggcaagctc
     2581 ctgcccattc tggaacagga tcatggtcgg gatcccataa accttcaggg catgtagaat
     2641 ttctggatgc tcgtcggcgt taatccgcca caggtcgacc ttacccttga attcttcgcc
     2701 cgtccggagc agccctgatt ccatggctct gcagggggca caccacgccg cccagaactc
     2761 cacgattacc gggagcgggt gtgcctgcag gcttgccagc aggctcgcct gccggctccc
     2821 cggcggatta tttagctcat ttcccatttc aggctttttt ggtactgaat ttcaggaggg
     2881 catagatcgg gcaccagccg ctcaacccgg tcagcagcgc cagcgcaccc acaatctcgg
     2941 ctacaatccc ccaggttccc gtcaccatcc taccaaaccc tgcgtataac agggcgacac
     3001 ccagcagtac ccgtacggac cgttcccaat tcgcttgatt gaccttcatg ttcgtctcct
     3061 ccagcttccg tttatttgtt ccagataaat actcaacgat tagatggaga atcgaccaaa
     3121 aggttacact catttaaaac gcaggggcgg agcagtgatc cgaacgccat ggatcgacgc
     3181 cagaaactgc tccgcccttt tcggaaacat cattattttg ttttcagaat gacctccggc
     3241 gatcactcct ctaccacttc gggtatgatc tgagccttaa aagtcaaccc ctcgctgccc
     3301 cggtcgacaa agatcgtgtc gccttcctgg aactcacccg ccagcagctt cagcgccagc
     3361 gggtcctgca gctcgcgctg gattgcccgc ttgagcggcc gcgccccaaa atcgggatcg
     3421 tagcccacct cggccaggta ctcccgggcc gccgggctga catccaggat gaacccgcgc
     3481 tctgccacga gctggacgga acgccgcagc tgaatttcca cgatctgcgc cagctgctca
     3541 cgccccaggc tgtggaacac cacaatctca tcgatccggt tgagaaattc agggcgaaaa
     3601 tgcgcttgca gtgtccgcgt caactcttcc cgtcccgctg tccgcccgcc ttcccagagc
     3661 tggttgccca ggttgctggt catgatcacc actgtattgc ggaaatctac cgtccggccc
     3721 tgtccgtcgg tcagccttcc atcgtccagc agctgcagca gcacattgaa gacttccgga
     3781 tgcgcctttt cgatttcatc gaacagcacc acgctgtagg gccgccgcct cactgcttcg
     3841 gtaagctgcc cgccttcttc atagccgata tacccgggcg gcgcccccac cagccgcgac
     3901 acggtatgct tctcctggta ttcgctcata tcgatgcgaa tcatcgcccg ctcatcgtcg
     3961 aacaagaatt ccgctaaagc ccgcgccagc tcagttttgc ctaccccggt cggtcccagg
     4021 aaaataaacg agccaattgg gcggttcggg tcttgcaggc ctgcgcgtgc cctccggact
     4081 gcgttcgata ccacccgcag cgcatcatcc tgccccacaa cccgcgcccg caggcgttcc
     4141 tccatgtgga ttaacttctc ggtctcaccc tccagcaggc ggctcaccgg gatgcctgtc
     4201 cagcggccta caatctgggc aacctcctcg gcgtccactt cttctttgag cagtgcgcct
     4261 tccttttgaa gcgctttgat gcgcagctcg ccctccgcca gcttgctttc cagctcgcgc
     4321 aggctcccat agcgcaaacg ggcggctttt tccaggtccg cctgccgctc ggcgcgctcg
     4381 atctcagtgc gcgtctgctc gatctgctct ttcaccgctt gcaggctggt aatggcctct
     4441 ttttccgtct tccagcgggt gtgcagctcg gtcgcgcgct cgcgcaggtc agcgacttct
     4501 ttttccagct tctccaggcg ctcccgggaa gctttgtcct tttctttttt caacgcctgg
     4561 cgctcgatct ccagctgcat gatctgccgg tccacttcat ccagggcctg cggcttggaa
     4621 tcgatctccg tccgcagccg cgccgccgcc tcatcgatca ggtcgatggc tttgtctggc
     4681 aagtgccggt ccgtgatata gcggttcgag agcgtcgcgg ctgcgatcac agccggatcg
     4741 gtgatccgca ccccgtgatg cacctcataa cgctctttca ggccgcgcag aatgctgatc
     4801 gtctcttcaa cgcttggctc ttctaccagc accggctgga agcggcgctc caacgccgga
     4861 tccttttcaa cgtgtttgcg gtattcgtcc agtgtggtcg ccccgatcag gtgcagctcg
     4921 cctcttgcca gcatgggctt gagcatattg ccggcgtcca ttgccccttc cgccgcgccg
     4981 gctcccacca ccgtatgcat ttcatctata aacagaatga tctcaccggc tgcatcgctg
     5041 atctctttca atactgcttt cagccgttct tcaaattcac cccggtattt ggctcctgcc
     5101 accagggcgc ccatatccag ctgcaccagc cgtttgtgtt tcaaaccttc cggtacatcc
     5161 ccgttgacca tgcgctgcgc caggccttcc acaatcgcgg tcttgccgac ccccgggtcg
     5221 ccgatgagcg ccggattatt cttcgttcgc cgcgagagga tctgcacgat ccgccggatc
     5281 tcttcatccc gcccgataac cgggtccatc ttgccctgcc gcgcctgggc agtcaggtcc
     5341 cgcccatatt tttccaggga ttggtaagtg gattccggcg tcgccgatgt cacccgctgc
     5401 gtgccgcgga tgccggtcag cgcctttagg atggcatcct tggtcagccc gtattgaccc
     5461 aggcgtgtac cttcgctgct ttccgtcagc cccagcagca ggtgctccgc cgagacataa
     5521 tcatcctgca tgcccttggc ataccgctcg gcagcttcca gcgctttggc ggtaggcggc
     5581 gccagcccga tatccccgct ggctccatag actcgtgagc gcgtctcaag ctccagggtc
     5641 agctcatccc gcagcgcc
//
LOCUS       DATMXK010000326         3273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_50474, whole genome shotgun sequence.
ACCESSION   DATMXK010000326 DATMXK010000000
VERSION     DATMXK010000326.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50474"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..331)
                     /locus_tag="VN363_06015"
     CDS             complement(<1..331)
                     /locus_tag="VN363_06015"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84102.1"
                     /translation="MQKAENILQAMWKLGENRIPLRRVYRCLFSEDLFLAAYDKIARN
                     RGALTAGTENDTVDGMNLARIRKIIEQLRYERFKFRPARRIQTPKKSGGTRPLGLSNF
                     TEKLVQEA"
     gene            complement(844..>3273)
                     /locus_tag="VN363_06020"
     CDS             complement(844..>3273)
                     /locus_tag="VN363_06020"
                     /inference="COORDINATES: protein motif:HMM:NF012493.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWQ84103.1"
                     /translation="ESRLPPLRFAPEPIYPLLPAPLKGAGGRAITFRRLDWAPDDLVE
                     ASRWSGVRKTRAWQDLVHPIESEVAFTPTMPLKKGHLAMLMASGMMGTLHLTSDDGYP
                     MLVKGRVVKVVDKVVHRDPEDPETVVERYRDRYVTTVATLSQQGLEMIQDVDALTRFM
                     RQYGDKIAAHTLETYQPLYGLKPTEAELVVLDRLGKTRKALPGQAEAGLLPTQKHAAI
                     AAARAIRQHRTANLQGEMGLGKTTIALAVIDLLNAYPALILCPPHLVPKWIREAEEVI
                     PGAKGRELRRIGKGEDGEINDVRKFFDDFDAGRLGPKAIAVVASTSAKMGAGWQAVVK
                     AKPIKVKGKAQVIYTCPQCGQVQLDPQNIPITEIEVFQKHRSFCNAQVSGWEIDRDGR
                     RKLDKDGNPVWGTRSCAAPLFEYSGVRRWSIAEYIKDKASQRFQILVGDEVHEYKAKA
                     SDRGVAFHQLVEASRYTLTLTGTYFGGKSTSIFWLLHRLSPAVRSDFAFHDELRWAGA
                     YGVLESVRKRRAGDETSDEDGAFTGNRRYREQAHEIPGVSPAIITRLLHNSIFLNLKD
                     LGVELPPYTEEVVELDMDNEHGRQYRGMESILRHMARRDPRYLSLWLQWALSRPNSAF
                     RDEIVAANHASVPVNVLEALAREMLENAATEDTLENQIARTLLEAARLAQSAATRREK
                     ETLVKVPLMPLEAVVDRFGLLPKESWLSSFCKAEKARGRRTLVYVRQTGTRDIQDRLE
                     GVLKQSGVQAITLYGSVDPRRRERWIEDHGYVDALITNPRLVQTGLDLVSFQTVVFYE
                     PEYSLVRRMAA"
BASE COUNT          602 a         1011 c          931 g          729 t
ORIGIN      
        1 aggcttcctg caccagcttt tctgtaaagt tcgagagacc cagcgggcgt gtccctccgc
       61 ttttcttcgg ggtttggatg cgacgggccg gacggaattt gaaccgttca tagcgtagct
      121 gctcgatgat ctttcggatg cgagccaggt tcatcccatc tactgtgtca ttctccgttc
      181 ccgcagtcaa agcgccacgg ttgcgagcaa tcttgtcgta cgcagccagg aacaagtcct
      241 cgctgaacag gcagcgatat actcgcctca ggggaatacg attttcaccc aacttccaca
      301 tggcttgtag aatgttttcg gctttctgca ttgcgcatac ctccaatttt ctagcaagct
      361 aaagttccta actcccttcg ccctgtggtt ggctttccca acctccatgg gtggacgtga
      421 tcccaccgac tactatggag tttccgtcgc catagggctc gcgccctgta ggcgatcccg
      481 cagtacggtc atgaatgacg ttatcaggat gacgtagggt gtccgttcgt ggcttgacag
      541 gtctcactga cccgtgttcc cggcaggagg atttcgcacc tctcagcctt tacaagatgc
      601 tgctgctggg gcttgacctc aaccgttgtg tcaagagagg aatagtctcc aactcgccat
      661 tggacttgaa gcagtttagc tttacccata tcatccaggt cttggggcga catcctacgg
      721 taaacggttt cccgcttcgc cccttttccg gcatgctatt ttcccgttcg ggtttcccct
      781 ttcggtcaac cggatgacct cgcaggtaat ctcctcaacc tgcggtacgt cacgtacaat
      841 cattcatgcc gccatgcggc gcacgaggct atactccggc tcatagaaca cgaccgtctg
      901 gaagctcacc aggtccagcc cggtctgcac caggcgggga ttggtgatca gtgcatcgac
      961 gtagccgtga tcttcgatcc agcgctcacg tcggcgaggg tccaccgagc cgtacagggt
     1021 gatcgcctga acaccggatt gcttgagcac accctccaat cggtcctgta tgtcacgcgt
     1081 gccagtctgg cgcacataca ccagtgtccg gcgaccacgc gccttttccg ctttacagaa
     1141 gctggatagc cagctttcct tgggcagcaa accgaaacga tccaccaccg cctcgagagg
     1201 catcaacggt accttgacca acgtttcctt ctcccgccgg gtagccgccg actgagccag
     1261 ccgagcagct tccagcaggg tgcgggcaat ctggttttcc aacgtatctt ctgtggcggc
     1321 gttctccagc atctcacgcg ccaacgcttc cagcacgttg actggcaccg atgcgtggtt
     1381 ggcagcgacg atctcatctc gaaaggccga atttggccga gagagagccc actgcagcca
     1441 cagcgacaga taacgcgggt cacggcgcgc catgtggcgc aggatgcttt ccatcccacg
     1501 gtactggcgt ccgtgctcgt tatccatgtc cagctcgacg acctcttcgg tgtagggcgg
     1561 tagctcgact cccaggtctt tcaggttgag gaagatcgag ttgtgcagca agcgggtgat
     1621 gatcgccgga ctgacgcccg ggatctcgtg cgcctgctcg cggtaacgcc ggttgccggt
     1681 gaaagcgcca tcttcatcgc tggtctcatc gccggcgcgg cgcttgcgca cactttccag
     1741 cactccgtat gccccagccc agcgcagctc gtcatgaaag gcgaagtcgc tgcgcacagc
     1801 tgggctcagg cgatgaagca gccagaaaat cgaggtgctc ttgccgccga agtacgtgcc
     1861 ggtcagtgtc agcgtgtagc ggctggcttc aaccagttgg tggaacgcaa ctccccggtc
     1921 gctggctttt gccttgtatt cgtgaacctc gtccccaacc aggatctgga aacgctggct
     1981 ggctttgtct ttaatgtatt cagcgattga ccaacggcgt acaccgctgt actcaaagag
     2041 cggcgcagcg caggaacgtg taccccatac gggattacca tccttatcca gcttgcggcg
     2101 accatcccgg tcaatctccc aaccagacac ttgtgcgtta cagaagctgc ggtgtttctg
     2161 gaagacctca atctcggtga tcgggatgtt ctgcgggtcc aactgcacct gaccgcactg
     2221 cgggcaagtg tagatgacct gggctttgcc cttcaccttg atcggcttcg ccttcactac
     2281 cgcctgccag ccggcgccca tcttggccga ggtagaagcc accactgcga tagccttagg
     2341 tcccaaccgg ccggcgtcga aatcatcgaa gaacttgcgt acatcgttga tctcaccgtc
     2401 ttcgcccttg ccgatgcggc gcagctcacg ccctttggct ccgggaatga cttcttccgc
     2461 ctcgcggatc catttgggca ccaggtgcgg cgggcacaag atcaaagccg ggtaggcgtt
     2521 cagcaggtcg atcaccgcca gagctatggt cgtcttgccg agccccatct ccccctgcag
     2581 attggctgtg cggtgctgac gaatggcgcg ggcggcagcg atggcagcgt gtttctgcgt
     2641 gggtagcagc ccggcttcgg cctgcccggg cagcgccttg cgggtcttgc ccaggcgatc
     2701 caggacgacc agttcggctt cggtgggctt tagaccgtag agtggctggt aggtctcaag
     2761 ggtgtgggca gcgatcttat cgccgtactg gcgcatgaaa cgcgtcagcg cgtccacatc
     2821 ctggatcatt tccagccctt gttgggaaag agtagccact gtcgtgacgt agcggtcgcg
     2881 gtagcgctcg acgaccgtct ccgggtcttc cgggtcgcgg tggacaacct tatctaccac
     2941 cttgaccacc cgtcccttaa ccagcatggg atatccatcg tcgctggtca ggtgcagggt
     3001 gcccatcatg ccggaggcca tcagcatcgc caggtggcct ttcttgagcg gcatggtggg
     3061 agtgaaagca acttcgcttt ctattgggtg aaccaggtct tgccaggcgc gtgtcttgcg
     3121 cacacccgac caacggctgg cttctaccag gtcatcgggc gcccaatcca gccggcggaa
     3181 ggtgatcgct ctgccgccag cgcccttcag aggggcaggt agcagtggat agatcggctc
     3241 aggggcaaac cgcagcggtg gcagtctgct ctc
//
LOCUS       DATMXK010000327         2625 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_24200, whole genome shotgun sequence.
ACCESSION   DATMXK010000327 DATMXK010000000
VERSION     DATMXK010000327.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2625)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2625)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2625
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24200"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            170..742
                     /locus_tag="VN363_06025"
     CDS             170..742
                     /locus_tag="VN363_06025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84104.1"
                     /translation="MSLYSLTLQGLPLRTGDVLCTHDGRRSSLFARFWRLLGLLIPGE
                     IDHCVIYLGPGGHYIESTARGVILFDMPGETWNPQLRASKRLLYDNLVGVAYPLEGRG
                     LSPAAAEEIRRGVAAYCLDKVSRRVPFNYNFFDPETDGAFYCSQLVYKAYLAQGLDLN
                     RNQGVPAAPGLDKVVFPQEIWNACIHRRVM"
     gene            968..2281
                     /locus_tag="VN363_06030"
     CDS             968..2281
                     /locus_tag="VN363_06030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84105.1"
                     /translation="MEMQERFHQKQNDPEGLEQLYQQALRQKSADDFSLAVQAAYTGA
                     PDNPLFAAWHYRLGAAVEVTRHILWKYAIPLSVLCGLIFWFLSDMKFEYLDAVPLLVL
                     LASPVAAIAVLAYLSLASRKGLGLAAGLSLGLIAFTAYALLMVPGMPSFFQDHAVTLM
                     LIHLPVLSLATVGIYLTGLSTGLNAQPQRRFAFVIKTFEVVTTGGLFAIAIAIFGAIT
                     FGLFDALGIQLSEVVTRLIFAGGGGMLPVLTVAIIYNPHQPPEEQDFSQGLSRFLANL
                     LRIMILPTLFVAVLYVLFIPFNFMEPFNNRDVLFIYNGMLFAVMALLVGATPIHTRDL
                     PQRLQVWLRYAILAVAALAVLVSLYALAAILYRTFMAELTMNRLTVIGWNVINISVLV
                     SLLFRQFSRAGSEWALGLKQAFNFAAVAYSVWAAFLVLAIPLLFR"
     gene            complement(2321..2458)
                     /locus_tag="VN363_06035"
     CDS             complement(2321..2458)
                     /locus_tag="VN363_06035"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84106.1"
                     /translation="MVTCVLGTLATTGTELELLNNQGILGVQLANELYLPAIGNWWAE
                     E"
BASE COUNT          464 a          826 c          723 g          612 t
ORIGIN      
        1 aggtgcacgg cggcgcgtcc gatgctgttt atggattggg tctttttggc gcctgggcgt
       61 attacatcgg ccgcgcaacc accccccggg aacgcgtgca gggattcttc aaaggtctcg
      121 tctggccggc tttcatggtc tacgcgctgt tccagttcct gaagaaagat tgagtctcta
      181 ctccctcacc ctgcagggcc tgccgctgcg caccggcgat gtgctgtgca cccacgatgg
      241 gcggcgcagc tcgctctttg cccgtttctg gcggctgctc gggctgctca tcccgggtga
      301 gatcgatcac tgcgtcatct acctgggacc cggcggccat tacatcgaat ccacggcccg
      361 gggtgtgatc ctgttcgaca tgcccggcga gacctggaat ccccaactta gagccagtaa
      421 acgcctgctc tacgacaacc tggtcggggt ggcttacccg ctggaaggcc gcggcctgag
      481 cccggcagcc gcagaagaaa tccgccgcgg cgtggccgcc tactgcctgg ataaggtcag
      541 ccgccgcgtc ccgttcaatt acaatttttt cgaccccgag accgatggcg ctttctactg
      601 cagccagctg gtatacaaag cctacctcgc ccagggccta gacctgaaca gaaaccaggg
      661 cgtacccgcg gctcccgggc tggacaaggt ggtcttcccc caggagatct ggaacgcctg
      721 catccaccgc cgggttatgt agcgcggact taaggcgaag gatcttttcg cccgtccgca
      781 tctggacagg cagatgctca tccaataccg ggtaccattc cgtaggttaa cctttagctt
      841 gaccttccag cctggatcaa gtggattgga ttccgcttaa gcacacaggt gcttgactgc
      901 cagcttgcat tttgctaagc ttaagcacca ttacaaatcc tttttgattc agcacctgga
      961 gaaaatcatg gaaatgcaag agcggttcca ccagaaacag aacgaccccg agggtctgga
     1021 gcagctctac cagcaggccc tgcgccagaa atctgccgac gatttcagcc tcgccgtcca
     1081 ggcagcctac accggtgccc ccgataaccc gctctttgcc gcctggcact atcgtttggg
     1141 tgctgcggtc gaagtgaccc gccacatcct gtggaagtat gccattccgc tgtccgtgtt
     1201 gtgtggcttg atcttctggt tcctttccga tatgaagttc gaatatcttg atgcggtgcc
     1261 gctgctggtc ttgctggcgt cgccggtcgc cgcgatagcg gtgctggcat acctcagtct
     1321 ggcttcccgg aagggtctgg gcttggcggc aggtctgtcg ctcggcttga tcgccttcac
     1381 cgcctatgcc ctgctgatgg tgcccggcat gccctcgttt tttcaagacc atgccgtcac
     1441 gctcatgctg atccacctgc ccgtgctttc attagcgacg gtcgggattt acctgaccgg
     1501 cttgagcacg ggcttgaacg cccagcctca gcggcgcttc gccttcgtga ttaaaacctt
     1561 cgaggtggtc accaccggcg ggctgttcgc catcgccatc gccatcttcg gcgcgatcac
     1621 tttcggcctg tttgatgccc tgggaattca gctgtctgag gtcgtaaccc ggctgatctt
     1681 tgccgggggc ggcggcatgc ttcccgtact gacggtagca attatctaca acccgcacca
     1741 gcctcccgaa gaacaggatt tcagccaggg attgagccgc ttcctggcca acctgctgcg
     1801 catcatgatc ctgccaacgc tgtttgtagc ggtgctgtac gtattattta tcccttttaa
     1861 tttcatggaa ccgttcaata atcgggatgt gctctttatt tataatggga tgttgttcgc
     1921 cgtgatggcg ttgctggtcg gcgcaacgcc catccacacc cgggatctgc cgcagcgcct
     1981 gcaggtctgg ctgcgctatg ctatcctggc tgtggctgcc ctggctgtac tggtcagcct
     2041 gtatgcactg gctgcgatcc tgtaccgcac cttcatggct gagctgacta tgaaccggct
     2101 gaccgtgatc ggctggaatg tgatcaacat cagtgtgctg gtctcactgc tgttccgcca
     2161 gttcagccgc gccgggagcg agtgggcgct agggctcaag caggctttca atttcgcagc
     2221 tgtggcctat tccgtgtggg ctgccttcct ggtgctggcg ataccgctcc tgttccgcta
     2281 accgctctga ggaacctgca ttctacgcgg acacatgggg tcattcttcc gcccaccagt
     2341 tcccgatcgc cggtaaatac agctcattcg ccagttgaac acccaggatc ccctgattgt
     2401 tcagcagctc cagctctgtc cccgtggtcg ccagtgtgcc caggacgcac gtaaccaccg
     2461 ccgcgatatg tagggcaggt attcaacccg ccaatcatca ccacgcctca cagggcagtc
     2521 ttttactccg ctccaggaat ctggcctgat tcatcgaaaa ccacagccag agcagcgcca
     2581 gcaggctgac actgctgaca atcagggtgt agctcccctc cgtcc
//
LOCUS       DATMXK010000328         4967 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_164002, whole genome shotgun sequence.
ACCESSION   DATMXK010000328 DATMXK010000000
VERSION     DATMXK010000328.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4967)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4967)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4967
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164002"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..289)
                     /locus_tag="VN363_06040"
     CDS             complement(<1..289)
                     /locus_tag="VN363_06040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84107.1"
                     /translation="MSSYPPYSSEPAEQPPYSGSPSSEPPRLQPVRASFSQETPVVVY
                     VIMGLTVVVYLLQMLTQFLFGGADIPAALGMKVNELIQQGQLWRLFTPMLLH"
     gene            complement(286..909)
                     /locus_tag="VN363_06045"
     CDS             complement(286..909)
                     /locus_tag="VN363_06045"
                     /EC_number="2.7.4.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561385.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="guanylate kinase"
                     /protein_id="HWQ84108.1"
                     /translation="MTDNNLAKYLVQEPLLIVISGPSGVGKDAVIKGMKDRQVEFHFV
                     VTATTRAPRPGEVEGKDYIFVSSGHFAEMINRNELLEYAVVYNDFKGVPKEQVRLALE
                     SGKDVIMRVDVQGAARIRELIPDTLLIFLTTQDEDELVKRLESRHTDTTTDRALRIAS
                     ARQEFKRIPEYDYIVVNRDGELDGAIDTILAIITAEHHRVEQRKVSL"
     gene            989..1384
                     /locus_tag="VN363_06050"
     CDS             989..1384
                     /locus_tag="VN363_06050"
                     /inference="COORDINATES: protein motif:HMM:TIGR02605.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FmdB family zinc ribbon protein"
                     /protein_id="HWQ84109.1"
                     /translation="MPTYPYRCLNCKKRFEIFMTYAEYGSLSVSCPHCGDSNVARRIN
                     RVRITRSTDSMLDNLDDPSSLDGLEDDPKAMGQMMRRMGAELGEELGPEFHEVVDRLE
                     SGQSPEEIEAALPDLGEPGGGAGGDDLDL"
     gene            complement(1381..2040)
                     /gene="tmk"
                     /locus_tag="VN363_06055"
     CDS             complement(1381..2040)
                     /gene="tmk"
                     /locus_tag="VN363_06055"
                     /EC_number="2.7.4.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR00041.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004798 - thymidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="dTMP kinase"
                     /protein_id="HWQ84110.1"
                     /translation="MFITFEGPDGSGKSTQVNHLVATLQRLGYAVYSTREPGGTPIGD
                     QIRSVLFDLQNTAMDPRTEILLFQASRAQHVSQVIRPRLERGELVLCDRFADSTLAYQ
                     GYGHCMDRAELQTIINFSTGGLQPDLTLLLDLPAEVGLSRREKGGDWNRLDAFTLAFH
                     CRVRQGYLEMAQADPQRWVVLNAELPPAELQAQIEHIVLDRINSRFQQPARPDRAAGH
                     S"
     gene            complement(2059..3732)
                     /locus_tag="VN363_06060"
     CDS             complement(2059..3732)
                     /locus_tag="VN363_06060"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558600.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="R3H domain-containing nucleic acid-binding
                     protein"
                     /protein_id="HWQ84111.1"
                     /translation="MEQRKITDDLQALMGVLPVSIAKAVTQADENDNLLEVIMDLGRL
                     PTARYVDREIGLRDQDVTHVDIDYVVDRIGDFDADNRAGLERTLHRISAIRNRRGQIV
                     GLTCRVGRAVYGTTDIIKDLIESGQSLLLLGRPAVGKTTMLREAARLLAETKRVVIVD
                     TSNEIGGDGDVPHPAVGRARRMQVATPSLQHEVMIEAVENHNPEAIVIDEIGRELEAA
                     AARTIAERGVQLIGTAHGNTLENLLLNPTLSDLVGGIESVTLSDEEARRRGTQKTVLE
                     RRSSPTFDVLIEIQDRNRLAVHPDVAAAVDSLLRGNLLPPEIRYRTSDGSIQIEKAPA
                     PVRLGSSGQQGYRRQANGYGSDGGRYPAREPSTAADRERSRQDAREAPEEWSPETAAY
                     PAPRGNHSITQTIRVYPYGVARNRLVQAARRLGAPVVVVNDLTEADILVTLRSYFRKR
                     ERTIVDAEQRDLPIYVLRANTINQMQQLFSDVFNLSMDSGVETGGMEPVVRETQQAIQ
                     SVLNGERWVEMPPAISAIRRLQHQMARQANLVSHSYGKEPFRRVRIYRE"
     gene            complement(3739..4683)
                     /locus_tag="VN363_06065"
     CDS             complement(3739..4683)
                     /locus_tag="VN363_06065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84112.1"
                     /translation="MGVRPFDWRDLNQLHRYRHQSIFLCSSLLLTRGPLLVPGALVSF
                     LAPSVGLITAAVEGNKKNGGSCLYGQTIQVNGAAYANLTFFTPEEALDSDLLPPMLDY
                     LAVQAGERGAFRILADVDEHSPVYESLRSASFAIYARQRIWKLHPPSTSGDPEPQGWR
                     KATNRDGLAIRALFNNMVPGLVQQIEPISTENPRGMLYYDKGELLAYVELKYGPRGVW
                     ANPLVHTDAEDVTPRLLDLFRNLPHQTSRQVYICVRSYQSWLESTLEELGATVGPRQA
                     VMVKHLAVNQKAGRLFSIPTLEGGQADLSAPMARVKHN"
     gene            complement(4692..>4967)
                     /locus_tag="VN363_06070"
     CDS             complement(4692..>4967)
                     /locus_tag="VN363_06070"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558598.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal 5'-phosphate synthase glutaminase
                     subunit PdxT"
                     /protein_id="HWQ84113.1"
                     /translation="AFGRQVASFETDINVPVLEQLAESDMPYHAVFIRAPLITGVHGD
                     ARVLASLEDGRIVAAQQGCWLATSFHPELTRDDRFHRYFLTLVEQGE"
BASE COUNT         1124 a         1399 c         1460 g          984 t
ORIGIN      
        1 ggtgcaagag catgggggtg aagaggcgcc agagctggcc ctgttggatg agctcgttga
       61 ctttcatccc cagggcggcg gggatatcgg ctccgccaaa caaaaactgg gtgagcatct
      121 gcaggagata gacaaccacg gtgagaccca tgatgacata gaccaccaca ggtgtctcct
      181 gactgaagct ggcccgaacc ggctggagac gaggcggctc cgaggagggc gacccagagt
      241 agggaggctg ctcagcgggc tcactgctat aaggggggta ggagctcata ggctcacctt
      301 tctttgctcc acgcgatggt gctcggctgt gatgatggcc aggatggtat caatcgctcc
      361 atccagctcg ccatctcgat tgacaacaat ataatcatat tcgggaatgc gcttgaattc
      421 ctgccgggca gaggcgatcc gtaatgcccg gtctgtcgtt gtatccgtat gtctggactc
      481 caggcgcttc accagctcgt cttcgtcctg ggtggtcagg aaaatgagca gcgtatcggg
      541 gatgagctca cgaatgcggg cagccccctg cacatcgacg cgcatgatca cgtctttgcc
      601 gctctccagg gccagccgga cctgctcttt tggcaccccc ttgaagtcgt tataaaccac
      661 ggcgtattcg agcagctcgt tgcgattaat catctcggca aagtgcccgg aggatacgaa
      721 gatgtaatct tttccttcaa cttcgccagg gcgcggggcg cgcgtcgtcg ccgtgaccac
      781 aaagtgaaat tccacctgac gatccttcat gcctttgatg acagcgtcct ttcccacccc
      841 cgagggaccc gagatgacaa tcaggagcgg ctcctggacg agatacttgg ctaagttatt
      901 atcagtcaca tgttcccctt tatgaatctg ccaatccaat tgggagttga ttataattcc
      961 cctgcacaat tttaatggag gaaatccgat gcctacttat ccataccgct gcttgaactg
     1021 caagaaacgg tttgagattt tcatgaccta tgccgagtac ggcagcctct cagtgagctg
     1081 cccacactgc ggcgatagca atgtcgcccg gcggatcaac cgggtgcgga ttacccgctc
     1141 gacagacagt atgctggaca atctggacga tccctccagc ctggacgggc tggaagatga
     1201 cccgaaggcc atgggacaga tgatgcgccg gatgggcgcc gagctgggcg aagaactggg
     1261 accagaattc cacgaagtgg tcgaccggct ggaatctggg caaagcccag aagagattga
     1321 agccgcccta cccgacctgg gggaacctgg gggcggagcg gggggagacg acctggatct
     1381 ttagctgtga ccggccgccc tatccggtct ggcaggctgc tggaaccggg aatttatccg
     1441 atccaacaca atgtgctcaa tttgagcctg gagctcagcc ggaggaagct ccgcattcag
     1501 caccacccag cgctgcgggt cagcctgagc catctccaga taaccctggc ggacccgaca
     1561 atggaatgcg agggtaaagg catccaggcg gttccaatcg ccaccttttt cgcgccgtga
     1621 aagcccaacc tccgccggga ggtccagcag aagggtcaaa tcaggctgca agcctccggt
     1681 cgaaaagttg ataatggtct gcagctcagc gcgatccata cagtgcccat acccttgata
     1741 ggctaaagta gaatctgcaa agcggtcgca cagcaccagc tcaccgcgct cgaggcgggg
     1801 gcggatgacc tgggagacat gctgggcgcg cgaggcctga aaaagcagga tctcggtgcg
     1861 cggatccatg gcggtgttct gcaagtcgaa gagtacactc cggatctggt cgccaatcgg
     1921 cgtaccgccg ggctcgcggg tggagtaaac cgcatatcca agccgctgca gggtagcgac
     1981 caggtggttg acctgggtcg atttgccgct cccatcgggg ccttcaaagg taataaacat
     2041 gccgagactc aaactccgtt attcgcggta gatacgcacc ctacggaaag gttcttttcc
     2101 ataggaatgc gagaccagat tggcctggcg ggccatctga tgctgcaagc ggcggatggc
     2161 agaaatagca ggcggcatct caacccagcg ctcgccattc aggacggact ggatcgcttg
     2221 ctgggtctcg cggaccaccg gctccatacc gcctgtttcc acgccagaat ccatagagag
     2281 gttgaaaaca tcactgaaca gctgctgcat ctggttgatc gtattggcac gcagaacgta
     2341 aatgggcagg tcgcgctgct cggcatcgac aatcgtgcgc tcgcgcttac ggaaatacga
     2401 gcgcaaggtc accaggatat cagcctcggt gaggtcgttg acaacgacaa ccggggctcc
     2461 caggcggcgg gcggcctgga ccagccggtt acgggcaacc ccatagggat agacacggat
     2521 cgtctgggtg atcgagtggt taccgcgcgg cgccgggtag gcggcagtct caggcgacca
     2581 ctcttccggt gcttcacgtg catcctgccg ggagcgctca cgatcggcgg cagtgctggg
     2641 ctcacgagcg ggataccgcc caccatcact gccatagcca ttggcctggc gacgataacc
     2701 ctgttggcca gagctgccca ggcgaaccgg ggcaggagcc ttttcgatct gaatactgcc
     2761 atcggaggtg cgataacgga tctctggagg caggagattg ccgcgcaaca aggaatcgac
     2821 tgcggcggca acatcgggat gtacagccag gcggttacga tcctggattt caatcaggac
     2881 gtcaaaggtt ggcgaagagc ggcgctcgag aaccgttttt tgagttccgc ggcgacgagc
     2941 ttcttcatca gagagggtga cggattcgat cccgccgacc agatccgata gggtgggatt
     3001 gagaagcaga ttctccagag tattgccatg ggcagtgccg atcaactgga caccacgctc
     3061 agcaatcgtg cgcgcagctg ccgcttcgag ctcgcgaccg atctcgtcga tgacgatggc
     3121 ttcagggttg tgattctcga cggcttcgat cattacttcg tgctgcaaag atggggtggc
     3181 tacctgcatc cgccgggcgc ggccgacggc gggatgaggc acgtccccat caccgccaat
     3241 ctcattggag gtatccacga tgacgacccg cttggtctct gccaggaggc gggcagcctc
     3301 acgcagcatg gttgtcttcc ccacggcggg acggcccagt aacaggaggc tttgaccgct
     3361 ttcgatcagg tctttgatga tatcggttgt gccgtagaca gctcgcccta cccggcaggt
     3421 gaggccgacg atctggccgc ggcgattgcg gatagcagag atgcggtgca gagtgcgttc
     3481 gaggccagcg cgattgtcgg catcaaaatc gcctatccgg tcgaccacat aatcgatatc
     3541 gacgtgggta acgtcctgat cgcgcaaacc aatctcgcga tcgacatagc gagcagtggg
     3601 caagcgcccc aggtccatga ttacttctaa taagttgtcg ttctcatcag cctgggtcac
     3661 tgccttagca atactgactg gcaaaactcc catcagggct tgtagatcgt ctgttatctt
     3721 acgttgttcc atattcactc agttgtgttt cacccgggcc atcggggcag ataaatccgc
     3781 ctgcccgcct tcgagggtgg gaattgaaaa taatcttccg gctttctggt tgactgccag
     3841 atgtttgacc ataacagcct ggcgagggcc taccgtggcg ccaagttctt cgagagtgct
     3901 ctccagccag gactgataag agcgcacaca aatatacacc tggcgtgagg tctggtgggg
     3961 caggttgcgg aacaaatcca gtagccgggg agtcacatct tctgcatcgg tgtggaccag
     4021 tggatttgcc cacacaccgc gcggtccata ttttagctca acataagcta aaagctcgcc
     4081 cttatcgtaa tacagcatac cgcggggatt ctccgtagag atcggctcga tttgctgcac
     4141 aagacctgga accatattat taaacagcgc ccggattgcc agaccatccc ggttcgttgc
     4201 cttgcgccag ccctgcggtt caggatcacc ggaagtggaa ggagggtgaa gtttccagat
     4261 gcgctggcgg gcatagatcg caaagctggc actgcgcaga gattcataga cgggactatg
     4321 ctcatcgaca tcggccaata tgcgaaaagc gccgcgctcg ccagcctgaa cagccagata
     4381 atccaacatc ggcggtagaa gatctgaatc cagggcttct tcgggcgtaa aaaaagtcag
     4441 attagcgtaa gcagcgccat tgacctggat tgtttgaccg tacaaacaag aaccaccatt
     4501 cttcttattt ccttccacgg cggcagttat caggccaacg gaaggagcca ggaaagaaac
     4561 caatgcgccg ggaaccaata aagggccgcg cgtcaagagc agcgagctgc acaagaagat
     4621 agattggtgc cggtaacggt gcagctggtt gagatcacgc cagtcaaacg gacgcacacc
     4681 catatacacg gctactctcc ctgctctacg agcgtcaaga aataacgatg gaaacggtcg
     4741 tcccgggtca attcaggatg gaaggaagtc gccagccaac acccctgttg ggcggcaacg
     4801 atcctgccat cctccaggct ggctaacacc cgggcatcac catggactcc ggttatcagg
     4861 ggggcgcgaa tgaaaacagc atgatacggc atatcactct cggccaactg ctcaagcacc
     4921 gggacgttga tatccgtctc gaaactggct acctggcgcc cgaatgc
//
LOCUS       DATMXK010000329         3989 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_155389, whole genome shotgun sequence.
ACCESSION   DATMXK010000329 DATMXK010000000
VERSION     DATMXK010000329.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3989)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3989)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3989
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155389"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            166..1440
                     /locus_tag="VN363_06075"
     CDS             166..1440
                     /locus_tag="VN363_06075"
                     /inference="COORDINATES: protein motif:HMM:NF013134.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD binding domain-containing protein"
                     /protein_id="HWQ84114.1"
                     /translation="MWREYINATSLEQVLDILAEQGAKARIVAGATDLILELERGVRT
                     GIEILVDISRVPGLDQITMDEDEIIHLGPLVTHNHCVASKLVRERALPLAKAAWEVGA
                     PQIRNRGTIAGNLITASPANDTITPLMALGAKLTLKSNHGERTVTLEEFYTGVRQTVM
                     QPDEVLVEISFPGLTDQQRGSFFKLGLRRAQAISVVNAAAIITFDPSEFAGKQKPKIK
                     AAAITLGAVAPTIIHASEAEAYLSGKELSTETIDHAARLAMQSAAPIGDVRSSADYRH
                     EMVRVSVLRALRQIQTHTERVGLPEHPVMLWGSTPCAETPALSAQACHEPGTPIITTI
                     NGKTYTFESGHTKTLLRLLREEAGLIGTKEGCAEGECGACTIFLDGSAVMACLVPAAR
                     AHMADIVTIEGLSYGDQLHPVQQAFLDTGAVQ"
     gene            1610..2017
                     /locus_tag="VN363_06080"
     CDS             1610..2017
                     /locus_tag="VN363_06080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84115.1"
                     /translation="MGKYSSYNRRTPLDERPWTIHPIWRGIGCVLMLLIPIMAYAGAI
                     LLVQANTRQGWIPMPSELTRTITLPWIGEVEHLFANMIVALLLALIGFALLSFFYSIF
                     YRLMNPDPLGPLDAKPVRSRPPAHPQGTRNQRR"
     gene            2040..3782
                     /locus_tag="VN363_06085"
     CDS             2040..3782
                     /locus_tag="VN363_06085"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84116.1"
                     /translation="MERTVPTTESESIDLYQRTYYSLLRSTSEVRIRTLEEVHAGMNS
                     LLHPAARAPEPDMSAFIYSLLRLPPEINDTRLVVMGQGKAVFTATGFRNVQEWQLVSA
                     SARRRRYYFDGTDILACVIASRSDIDDLIPMLIAYQLEWNKIHQRLQRLPENSHVAEL
                     CNTAEGYQMVLDVLNIPEEDLDRLSVIWDENFASNLEKIQQTSRDLRVRLLSSSLTEY
                     RRATRAWWDQIVKTLPSLRERPVYFVSSNTHSILNLLTSHAISNQEQILHYLKRSGNK
                     GLLGEWQDIQSGRSTTDANNFLYYVQKKYQQTPEGIHQTQTQVLNEQDCGILRIPSEN
                     FFDVEAQLVDLSRLGCAHLDPRLQFPDAPNAGDLFSTSQALILNVDYPLGLGAYQILS
                     EIAETVGSILGIYVMGKAATLNAVAGDVMIPNVVHDEHSRNTYLFANCISAGDVTPYI
                     SIGTVLDNQKAVTVQGTFLQNTRYMDVFYSEGYTDIEMEAGPYLSAVYEMFRPQRHPV
                     NEIVNLYGLGFDLGIIHYASDTPLSKGKNLGARSLSYSGMDATYASSIAILNRIFKME
                     QERLFHLTNYRTTA"
     gene            complement(3764..>3989)
                     /locus_tag="VN363_06090"
     CDS             complement(3764..>3989)
                     /locus_tag="VN363_06090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560130.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWQ84117.1"
                     /translation="VKDRSTREPLAPYNGSSPEMARIKKYLQEHGLFLSQHWHTLLII
                     PPLIITEAQLSEGFSIIDQALEIADQAVVR"
BASE COUNT          948 a         1073 c         1044 g          924 t
ORIGIN      
        1 gtgagcattc tggcagagat catcatgctc cgccagggag gcgatggacg ggacatggac
       61 acgtctcaca gtaaagccaa aatatccaga cgcaaataat agattcttta ttaatctgat
      121 caatagcaat ctttagctga taaactgatt tcgacggagt gatttatgtg gcgtgaatac
      181 atcaatgcaa ccagcctgga gcaggttctg gacatcttag cagaacaggg tgctaaagcg
      241 cggatagtcg ctggggcaac tgatttgatc ctggagctgg agcggggtgt acgaacgggg
      301 atcgagatat tggttgatat ttcgagagtg cccgggttgg accaaattac aatggacgaa
      361 gatgaaatca tccatctggg tccactggtc actcacaacc attgtgttgc ctccaagctg
      421 gtgcgagaac gtgcccttcc cctggcaaag gctgcctggg aagtcggtgc gccgcaaatc
      481 cgcaaccggg gtacgatcgc cggaaacctg atcactgctt cgcctgccaa cgataccatc
      541 acgccgctga tggcgctagg cgcaaaatta accctcaaat cgaaccacgg cgagcggact
      601 gtgacccttg aggagtttta caccggtgtc cgccagaccg taatgcagcc cgacgaggtt
      661 ctggtggaga tttcttttcc aggattaaca gatcagcagc ggggatcctt tttcaagctg
      721 ggtctgcgcc gtgcccaggc aatttcggtt gtcaacgccg cggcgattat cactttcgat
      781 ccatccgaat ttgctggaaa acagaaacca aaaataaaag ccgctgccat cacactcggt
      841 gctgttgctc ccacgataat tcatgcaagc gaggcagagg cttatcttag cgggaaggag
      901 ctgtccactg agaccatcga tcacgcagcc aggctggcga tgcagagtgc cgctccgatt
      961 ggggatgtgc gcagctcggc ggattaccgg catgagatgg tgcgcgtttc cgtcctgcgc
     1021 gccctgcgcc agattcaaac tcacacggaa cgcgtgggac tgcccgaaca tccggtgatg
     1081 ctgtggggga gtactccttg cgctgaaacc ccggcactct ctgctcaagc ctgccacgag
     1141 cctggcacac cgattatcac cacgatcaat ggcaaaacct atacattcga aagcggacac
     1201 accaaaactc tcttgcgctt gctccgtgaa gaggccggtc tgatcgggac gaaggaaggc
     1261 tgcgctgaag gcgaatgtgg tgcctgtacg attttcctgg atggcagtgc cgtgatggcc
     1321 tgtctcgtcc cggctgcccg agcccatatg gcagatattg tcacgatcga aggtctgtct
     1381 tatggcgatc agcttcaccc ggtccagcag gcatttctgg ataccggcgc cgtccaatga
     1441 ggttactgca caccggggtt tgttatgtca ggagccaagc tgctggaaga acgtccccat
     1501 ccgagtaccg aggacattaa attcgctatc acgggtaatt tatgccggtg taccgggtat
     1561 tataagattg tagaggcaat cgaacgtgcc agccatccgg aggtaagcta tgggtaagta
     1621 ttcctcgtat aatcgccgca ctcccctcga cgaacgtccc tggacgatcc acccgatctg
     1681 gcgtgggata ggttgtgtgc tcatgctatt gatcccaatc atggcatacg ccggtgcaat
     1741 tctgctggtt caggcaaata cccggcaggg ttggattccc atgccatctg agctgacacg
     1801 aacaataacc ttgccctgga tcggcgaggt tgaacaccta tttgcgaaca tgattgttgc
     1861 tctactgctg gcgctgatag gattcgcctt gctgagcttt ttctactcga ttttctaccg
     1921 cttgatgaat cccgatccac ttggtccact ggatgcaaaa ccggtccgct cgcgcccccc
     1981 ggcgcatcca cagggaaccc gtaaccagcg ccgataattt tacctgcgaa taaaaattca
     2041 tggaacgtac cgtcccaacc accgaatccg agagtatcga tctttaccaa cgcacctatt
     2101 attcgctcct gcgctcgacc agcgaggtgc ggatccgcac cctggaagaa gtccatgcag
     2161 ggatgaattc actgctccat ccggcggcgc gcgctcccga accggatatg tcagcattta
     2221 tctacagcct gctccggctg ccgcctgaga tcaacgatac ccggctggtt gtaatggggc
     2281 aggggaaagc ggtcttcacg gcgactggtt tcaggaatgt ccaggaatgg cagctggtga
     2341 gtgcatctgc ccgcaggcgg cggtattatt tcgatggcac agatatcctg gcctgtgtca
     2401 ttgcaagccg ctccgacatc gatgatctaa ttcccatgct gatcgcttat cagctggaat
     2461 ggaataagat ccaccagcgg ttgcagcgac ttccggaaaa ttcccatgtc gccgagctgt
     2521 gcaatactgc agagggttat cagatggtgc tggatgtcct caacatccct gaagaagatc
     2581 tggaccgcct ctcagttatc tgggatgaaa atttcgcctc taatttagag aagatccaac
     2641 aaacttcccg cgatttacgc gtacgcctgc tcagcagctc gctaactgaa taccgccggg
     2701 cgacacgcgc ctggtgggac cagattgtca agacactccc cagcctgcgc gagcgaccag
     2761 tttattttgt ctccagcaac acgcacagca tattgaattt acttaccagc cacgcgattt
     2821 ccaaccagga gcaaatcctg cattatttaa agcgctctgg taacaagggt ttgctcggcg
     2881 aatggcagga tatccaatct ggacgatcta cgaccgatgc caacaatttt ctttattacg
     2941 tccagaagaa ataccagcaa actcccgaag gtattcacca gactcagaca caggtgctca
     3001 acgagcagga ttgtggcatc ttacgcatcc ccagtgaaaa cttttttgat gtcgaagctc
     3061 agctggtcga cttaagccgc ctgggttgtg cacatttgga cccgcgcctg cagtttccgg
     3121 atgcccccaa cgcaggcgat ctgttttcaa ccagccaggc attgatcctg aatgttgatt
     3181 atccgctggg tctgggggca tatcaaatcc tgagcgaaat tgcggaaacc gtagggtcga
     3241 tcctgggtat ttatgtcatg ggcaaagccg ccacattaaa cgctgtggcc ggcgatgtga
     3301 tgatacccaa tgttgtgcac gacgagcatt cccggaacac ttacctgttc gccaactgca
     3361 tcagtgccgg agatgtgacg ccatatatct cgatcgggac agttctcgac aaccagaaag
     3421 ctgtcaccgt gcagggcact tttctgcaaa acacacgcta tatggatgtt ttttacagcg
     3481 aggggtatac ggatattgaa atggaagcgg ggccttatct ttcagcggtc tatgaaatgt
     3541 ttcgacctca gcgccatccg gtcaatgaga ttgtcaacct gtatgggctg gggttcgatc
     3601 tgggcatcat ccactatgcg tcggatactc cattaagtaa agggaaaaat ttaggagcgc
     3661 gcagcctttc ttactccgga atggatgcta cctacgcttc cagcatcgcc atcctgaacc
     3721 ggatcttcaa aatggagcaa gagcgcctct ttcacctcac gaattaccgg acgaccgctt
     3781 gatcggcaat ctccagagcc tgatcgataa ttgaaaagcc ttcactgagc tgagcctcgg
     3841 tgataatcaa cggcgggata atcagcaagg tgtgccagtg ctggctgaga aataatccat
     3901 gttcctggag atatttttta attctggcca tctcggggct gctgccatta taaggcgcca
     3961 gaggttcccg cgtgctgcga tctttaacc
//
LOCUS       DATMXK010000330         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_38020, whole genome shotgun sequence.
ACCESSION   DATMXK010000330 DATMXK010000000
VERSION     DATMXK010000330.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38020"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..951)
                     /locus_tag="VN363_06095"
     CDS             complement(<1..951)
                     /locus_tag="VN363_06095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84118.1"
                     /translation="MPSISDPFSQRRSRAFITAASYALVCGMLMMATITIVQAMTVLA
                     PGWQAGAWVWLSPLLALEALLSARQMRQYPALTPEWLAYRLAEWVTILLILRLVLYGV
                     EGLDVLRADLSLPWREFASHFFSGAYWAWVVLGLLVWLVAGEFSASLEPLEVDEQAVE
                     MERDAHAGPERQQARTRLAVLILSVGGVLLLAAALLRQDWVARLGNQPPLTAGAMNLV
                     VYFVLGLLLLSLTRFSMLAMRWGINEYPVRTEVPQRWLAYGLLFLAVLGGIALLLPTA
                     YAEGLLRVLAVVINFLAVVIAYLIYLISIPFLFLLNSLMHL"
     gene            complement(955..2142)
                     /locus_tag="VN363_06100"
     CDS             complement(955..2142)
                     /locus_tag="VN363_06100"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWQ84119.1"
                     /translation="MGNLLTVFILFIAGLAVFLQADFALTVLYLLGGVILFGQWWSRQ
                     AMRHLEMRRQFATHAFQGEAIPVRLEIHNPGLLPIPWLQLHESLNVAISPRPAYQEVF
                     SLPGRASRSFEYSLEGRKRGYFPVGPLTLASGDLFGLLKDLQMVLPDEHLTVYPRIIP
                     LHRVDLPSRSPLGALRTHQPVFEDPARVWGKREYTRGDSLRRIDWKASAASDRLQVKQ
                     YEPSIALEAVVCLDLHNGDYASRSRFYNGELAITTAASLASWITGARQAVGLLANGRD
                     SLQEDPGPQWLGARRGRAHLMHILEVLARLELADTRPLTETLSQQIHTLPWGTTLIVI
                     TPVLDDALFESLFQARRQGLNLFVVGIGPVPGYAAARHKAQHFGIPLHAVQAESDLDQ
                     WCQ"
     gene            complement(2161..3138)
                     /locus_tag="VN363_06105"
     CDS             complement(2161..3138)
                     /locus_tag="VN363_06105"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWQ84120.1"
                     /translation="MNEIQQFSQQLVSHVEQVIVGKRSVIELVTVALLCEGHVLLEDV
                     PGTGKTMLGRALAISLGGSFKRLQCTPDLLPNDVTGVSVFNQGQADFEFRPGPVFVNI
                     LLADEINRATPRTQSALLEAMQEQQVTVDGVTRPLPRPFLVLATQNPIEYEGTFPLPE
                     AQLDRFLMRLPLGYPSPADERQILLNLRREHPIASLQPLAFDLDGGSLSGVEALLRLQ
                     RQVWEVHVDSSLEDYLLRLVGATRQHPELALGASPRASLALLRAAQALAAVRGRDHVL
                     PDDIKYLTPVVLPHRLLARPEAELRGRTAGLVLADLLNSLPLELDAPAP"
     gene            complement(3145..3441)
                     /locus_tag="VN363_06110"
     CDS             complement(3145..3441)
                     /locus_tag="VN363_06110"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84121.1"
                     /translation="MSLPLPRVVIIRAGFASLSAVLGHSARSSCPGRRAARRLAGVAG
                     GAALLVNWLPQPPAGDDQLGLGLFIYERAVRLINSQSTIDLDQMPSPPDPNSPG"
     gene            3577..4311
                     /locus_tag="VN363_06115"
     CDS             3577..4311
                     /locus_tag="VN363_06115"
                     /inference="COORDINATES: protein motif:HMM:NF013570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanyl-tRNA editing protein"
                     /protein_id="HWQ84122.1"
                     /translation="MTELLYQTDSYLQTFTATVTGVDPEAHAVILDRTAFYPSGGGQP
                     SDQGSLSGLDGTLAGQSLRVEKVKKQGGEVLHFLDPAAPLPEMGAQVRGQLDWERRYQ
                     HMRTHTAMHILCGVIFRDYGASVTGGDMDPLKGRMDFEFETMHADLVGKIEAAINIEV
                     AAGREVRTAILPRDEAFQIPDLIRTKINLLPEGIQQVRVVEIIGLDLQADGGTHVHNT
                     REVGRMRVVDYKSKGKINKRIYVELD"
     gene            complement(4413..>5474)
                     /locus_tag="VN363_06120"
     CDS             complement(4413..>5474)
                     /locus_tag="VN363_06120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84123.1"
                     /translation="VESGAGVTLEGVNYMNLDAIVRLTAQAPGTAVRDVETQVFGDLD
                     TPATEVVDGTTRFISDCRVHDRMTFVVPEDMPPGLYTLQILMPNTTGIPILGDPIASD
                     IQFIRVIPPSTARFQITLERMNCRKETSPAWFGSDEVGLSIVAGALLPDGITEIQALK
                     NSDGKDFLRFDDVDSGDKRPIERLVFNQQQPILAVAMLVLGYEVDSERAFEEQITSGT
                     DFFVELLKEQWNFIKDNELVKAALKGLVSKFGIYGIIAIAIAAVVLVAVDAIIAGWAP
                     ADPIMQETFGFSVSDLAQLTSVNFPTPGFGHFDATDDISVDTTPLEKLPNQYREFREY
                     ASDDEDSRYDLFFRYNRTA"
BASE COUNT         1191 a         1617 c         1734 g          932 t
ORIGIN      
        1 cagatgcatc agagaattca gtaagaacaa aaatggaatc gaaataaggt agatcaggta
       61 agcaatgacc accgccagga agttaatcac gaccgccagg acgcgcagca agccctcagc
      121 atacgctgtg ggcaggagta aggcgatccc gcccagcaca gccagaaaaa gcagcccgta
      181 agccagccaa cgctgtggca cttcggtccg cacaggatat tcattaatcc cccaacgcat
      241 ggcgagcatt gagaaccggg tcaggctcaa caggagcagc cccaggacaa aataaaccac
      301 cagattcatc gccccggcag tcagcggcgg ttgattcccc aggcgcgcca cccaatcctg
      361 gcgcagcagc gcggctgcca ggagcagcac accgcctacc gaaagaatca acactgccag
      421 gcgggtgcgc gcctgctggc gctcagggcc ggcgtgcgca tcgcgctcca tctccactgc
      481 ctgctcgtcc acttccagcg gctccagact ggcagaaaac tcaccggcga ccagccagac
      541 taacaggccc agaaccaccc aggcccagta agccccactg aaaaaatggg aagcaaattc
      601 acgccagggc aggcttaaat ccgcccggag aacgtcaagg ccttcgaccc cgtaaaggac
      661 caggcgcaag atgagcagaa tggtgaccca ctctgccagg cggtaagcga gccattcggg
      721 tgtcagggca ggatactgcc gcatctggcg ggcggagagg agggcttcca gcgccagcaa
      781 gggactgagc cacacccagg ctccggcctg ccagccaggc gccaggacag tcattgcctg
      841 gacgatcgtg atggtcgcca tcatgagcat cccacacacc aacgcgtaac tggcagctgt
      901 gataaaagcc cgtgagcggc gctgagagaa gggatctgaa atgctgggca tggcttactg
      961 gcaccactgg tccaggtcgg attcagcctg gacagcatga agcgggatac cgaaatgctg
     1021 ggctttgtgg cgcgcggcgg cataaccagg gaccggacca atgcctacca cgaacaaatt
     1081 caatccctgg cggcgagcct ggaaaaggct ctcgaacagt gcatcatcca gaaccggagt
     1141 gatgacgatc agggtagtgc cccacggcaa ggtgtgaatt tgctggctaa gggtctctgt
     1201 cagcggccgg gtatcagcca gctccaggcg ggcgaggact tccaggatat gcatcaggtg
     1261 tgcccggccg cgccgggcac ccagccactg cggtccaggg tcttcctgga ggctgtcccg
     1321 cccattggcg agcagcccca ccgcctgccg ggcgccggtg atccagcttg ccagggaggc
     1381 ggctgtggta atcgccagct caccattata gaagcggctg cgagacgcgt aatcgccatt
     1441 atgcaggtcg aggcaaacca ccgcttcgag ggcaattgac ggctcgtact gcttgacctg
     1501 caatcgatct gaggctgcac tggctttcca atcaatccgg cgcagcgaat cgccgcgggt
     1561 gtattctcgc ttcccccaca cccgggcagg gtcctcaaaa accggctggt gcgtgcgcag
     1621 agcacccagc ggtgatcttg aaggcagatc gacccggtgc agggggatga tgcgcgggta
     1681 aacggtgagg tgttcgtcgg ggagaaccat ctggagatct ttgagaagtc cgaacagatc
     1741 accggatgcc agggtcaggg ggccgaccgg gaaatagccg cgcttgcggc cttccagtga
     1801 atactcaaag ctgcgcgaag cgcgccctgg caggctgaag acttcctggt aagcggggcg
     1861 cgggctgatg gcaacattaa ggctctcatg cagctgcagc caggggatag ggagaaggcc
     1921 ggggttgtgg atctccaggc ggacggggat cgcttcgccc tggaaggcat gtgtcgcaaa
     1981 ctggcgccgc atctccaggt ggcgcattgc ctgccggctc caccattgac cgaacaggat
     2041 cactccgccc agcaggtaga gtacggtcag ggcaaaatcc gcctgcagga atacagccag
     2101 gccggcgata aagaggataa agacggtgag tagattcccc attcaggcac ccgatcgggt
     2161 tcagggggct ggggcgtcca gctccagagg caggctgttc aacagatccg ccaggaccag
     2221 cccggcggtc cgtccgcgca gctcggcctc ggggcgcgcc agcaggcggt gcggaagcac
     2281 caccggggta aggtatttga tatcgtccgg caggacgtga tcgcggccgc gcacggctgc
     2341 cagagcctgg gcggcccgca gcaaggccag gctggcgcgc gggctggcgc ccagggccag
     2401 ctcggggtgc tggcgtgtgg cgccgaccag gcgcagcagg taatcttcca ggcttgaatc
     2461 cacgtgcacc tcccacacct ggcgctgcag gcgcagcagg gcctccaccc cgctcagcga
     2521 gccaccgtcc aggtcaaatg ccagcggctg caggctggcg atagggtgct cacggcgcag
     2581 gttaagcagg atctggcgct cgtcggcagg ggagggataa cccagcggca gacgcatcag
     2641 aaatcggtca agctgggctt caggcaaggg gaaggtgcct tcgtattcga tcggattctg
     2701 ggtcgccagg acgaggaacg gtctcggcag cgggcgggta accccatcca cagtgacctg
     2761 ctgctcctgc atggcctcca gcagggcaga ttgggtgcgg ggcgtggcgc ggttgatctc
     2821 atctgccagc aggatattga caaacaccgg cccgggtcta aactcgaaat ccgcctgccc
     2881 ctgattgaaa acagagacgc cggtgacatc gttgggcagc aggtcgggcg tgcactgcag
     2941 gcgtttgaag ctaccgccca gggagatcgc cagggcgcgc cccagcatag tcttgcccgt
     3001 gccaggaaca tcctcgagca ggacatgccc ctcgcacagc agcgcgacgg tgaccagctc
     3061 gatcaccgaa cgcttgccga cgatcacttg ctcgacatgg gagaccaatt gctgcgagaa
     3121 ctgctggatt tcgttcatgg gcgtttatcc gggtgaattg ggatccggtg gagagggcat
     3181 ctggtctaaa tctattgtac tctgagaatt gatcaggcgc acggcccgtt cgtaaataaa
     3241 taatcccagg cccagttgat catcaccggc cggcggttgt ggaagccaat taaccagaag
     3301 agctgcaccg ccagccacac cagccaggcg gcgggctgcc ctccgaccag ggcagcttga
     3361 ccgtgctgag tggcccaaga cggcgctcag gctggcaaag ccagcccgga tgatcacaac
     3421 cctgggaaga ggtaaggaca tgcataccta tactggagaa ttacatgatc aattctatcc
     3481 gatttcattg aaatttaaac gctattgcag ctgctttctg gtaaattact gctgtatggt
     3541 tataatcaga gcaaatctcc caactgaggt gcaaccatga ccgaactcct ctaccagacc
     3601 gacagctacc tgcagacctt caccgccacg gtaaccggcg tcgaccccga ggcccacgcc
     3661 gtgatcctgg accgcacggc cttctaccca tccggcggcg ggcagccctc cgaccagggc
     3721 agcctgagcg ggctggatgg cacgctggca gggcagagcc tgcgggtgga aaaggtcaaa
     3781 aagcaaggcg gggaggtttt acatttcctc gatccggcgg cacccttgcc ggagatgggc
     3841 gcacaggtgc gcgggcagct cgactgggag cggcgctacc agcacatgcg cacgcacacc
     3901 gccatgcaca tcctgtgcgg ggtgatcttc cgcgattacg gagcctcggt caccggcgga
     3961 gatatggacc cgctcaaggg gcggatggat ttcgagttcg agacgatgca tgccgacctg
     4021 gtgggtaaga tcgaggcggc gatcaatatt gaagtggctg ccgggcgaga agtgcgcacg
     4081 gcgatcctgc cgcgggatga agccttccag atccccgacc tgatccgcac gaaaatcaac
     4141 ctgctgccgg agggcatcca gcaggtgcgc gtggtggaga ttatcggcct ggaccttcag
     4201 gcagacggtg ggacgcacgt gcacaacacc cgcgaggtgg ggcgcatgcg ggtggtggat
     4261 tataagtcca agggcaagat caacaagcgc atttacgtag agctggatta agtcaaatta
     4321 accctgcgag actccaaacc ccaggggtaa atccgtcgga tttttaaatc ctacggattt
     4381 ttcaaacccc aggggtttgg tattaaatat tcttacgccg tgcggttgta gcggaagaac
     4441 aggtcgtagc ggctgtcttc gtcgtcgctg gcgtactcgc gaaactcacg gtactggttg
     4501 ggcagcttct ccagcggggt ggtatccacg ctgatatcgt cggtggcgtc gaagtgacca
     4561 aagcccggag tggggaagtt gacgctggtg agctgggcaa gatcggagac gctgaagccg
     4621 aaggtctcct gcatgatcgg atcggcgggt gcccagccgg cgataatggc atcgacggcg
     4681 acgagcacga ctgcggcgat ggcgatggca atgatgccat aaataccgaa tttactgacc
     4741 agccctttga gggctgcttt caccagctcg ttgtccttga tgaaattcca ctgctccttc
     4801 aacagctcga cgaagaaatc ggttccgctg gtgatctgtt cctcgaaagc ccgctcactg
     4861 tccacctcgt agccaaggac cagcatcgcc accgccagga ttggctgctg ctggttaaac
     4921 accaggcgct cgatggggcg tttgtccccc gaatcgacat cgtcgaagcg cagaaaatcc
     4981 tttccgtcgg agttcttcag cgcctggatt tccgtaatgc catccggcag cagggcgccg
     5041 gcgacgatgc tcaggccgac ctcgtcggag ccaaaccaag ccggggaagt ctctttgcgg
     5101 cagttcatac gctcgagggt gatctggaag cgggcggtgg agggcgggat gacgcggatg
     5161 aactggatgt ccgaggcgat cggatcgccc aggatcggaa taccggtggt gttgggcatc
     5221 aggatctgca gggtgtacag accggggggc atatcttcgg ggacgacaaa ggtcatccgg
     5281 tcgtgcaccc ggcagtcgga gatgaagcgg gttgtaccgt ccaccacctc ggtcgccgga
     5341 gtatccagat cgccgaagac ctgggtttcc acatcccgca cggcggtgcc cggcgcctgg
     5401 gcggtgaggc gcacgatggc atccaggttc atatagttca ccccttcgag ggtcaccccg
     5461 gcgccgctct ccac
//
LOCUS       DATMXK010000331         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_150382, whole genome shotgun sequence.
ACCESSION   DATMXK010000331 DATMXK010000000
VERSION     DATMXK010000331.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_150382"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1288)
                     /locus_tag="VN363_06125"
     CDS             complement(<1..1288)
                     /locus_tag="VN363_06125"
                     /inference="COORDINATES: protein motif:HMM:NF013528.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CBS domain-containing protein"
                     /protein_id="HWQ84124.1"
                     /translation="MRIILTHEQADFDALASLLAAHLTDPDSTPVLPWRMNRNVRAFL
                     TLYGHELPFIEQRELNGEPISAVTLVDTQSMATVRGVVSNPVVRVIDHHPRRDSLSPN
                     WETWISEIGATTTVLTETLQEQNVLLTPIQATLMMLGIYEDTGSLTYAHTSPRDILAA
                     GFLLAQGANLSVLNDFLNHPLSKEQQVIYDELREAVENLQIHGYTILLALGDAHGTDE
                     ELSSIAHKLRDLYDPAAIFLLVQTRSGVQMIARSNTDHIDVGEISEAFGGGGHDRAAA
                     ALIHDRDLQDIRTELIEMLPVYIRPAVTVSQIMSRIPQVLTPDTSDQEAARRMSLYGY
                     EGYPVVENGQVVGLLTRRQVDRAIAHKLNYTARQLMSAGQITVSADDSIEQLQEKMIN
                     SGWGQIPVVDPHSQAIIGIVTRTDLLKTLPGHSRPPT"
     gene            1442..1759
                     /locus_tag="VN363_06130"
     CDS             1442..1759
                     /locus_tag="VN363_06130"
                     /inference="COORDINATES: protein motif:HMM:NF015758.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047429 - nucleoside triphosphate
                     diphosphatase activity [Evidence IEA];
                     GO_process: GO:0009143 - nucleoside triphosphate catabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MazG-like family protein"
                     /protein_id="HWQ84125.1"
                     /translation="MAAMNLNDLTIAMHEFVRSQGWYEAGTRRPQTLRNLAISLNLEA
                     SEVLEHFQWRENADHPDELGSELADVALYLLQIASIAGIDLEQAILKKLEVNQLRKWD
                     QEE"
     gene            1756..>2013
                     /locus_tag="VN363_06135"
     CDS             1756..>2013
                     /locus_tag="VN363_06135"
                     /inference="COORDINATES: protein motif:HMM:NF037785.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84126.1"
                     /translation="MSNITVFGGSQIQPGMPDYLAAQRLGGLLAMAGHCVLTGGYIGA
                     MEAVSRGAAEAGGHVIGVTCAEIEAWRPGKANAWVKEERCFP"
BASE COUNT          464 a          503 c          575 g          471 t
ORIGIN      
        1 gtgtgggggg tctggaatgc ccgggcagag tcttgagtaa gtcggttcgg gtgacaatcc
       61 cgatgatcgc ctggctatgg ggatcgacaa ccgggatctg cccccagccg ctgttgatca
      121 ttttttcctg aagctgctcg atcgaatcgt cagcagagac ggtaatttga ccggctgaca
      181 ttaactgccg ggctgtgtaa tttaatttgt gggcgatggc gcgatccacc tgacggcggg
      241 tgagcagccc gaccacctgt ccattctcga caaccgggta accttcatac ccatacaggc
      301 tcatccggcg ggcagcttcc tggtcgctgg tgtcaggtgt taatacctgg ggaatccggg
      361 acatgatctg gcttacggtt acagcgggtc ggatatacac tgggagcatt tcgatcagct
      421 cagttcggat atcttgcaga tcgcgatcat gaattaaggc tgctgccgcg cggtcatgac
      481 cgcctccacc aaatgcctcg ctaatttcac cgacatcgat atgatcggtg tttgagcggg
      541 caatcatttg cactccactg cgcgtctgaa ccagcaaaaa aatggcggct ggatcgtaca
      601 ggtcacgcag cttgtgggcg attgaagaga gctcttcgtc tgttccatgg gcatcgccca
      661 acgccagcag gatcgtatac ccatgaattt gaagattttc gaccgcctcg cgcagctcgt
      721 cgtagatgac ctgttgctct ttggaaagcg ggtgattcaa aaaatcattc aggacgctca
      781 aattagcacc ctgagccaaa aggaaaccgg ctgccaggat atcgcgaggg ctggtgtggg
      841 cataggttag tgagccggta tcctcgtaga tacccagcat cattaatgta gcctggattg
      901 gagttagcag gacattctgt tcctgaagtg tttcggtgag aaccgtggtt gtcgccccga
      961 tctcagaaat ccaggtttcc caatttggag ataggctgtc ccgccgcggg tggtgatcga
     1021 tcacccgcac aaccgggttt gataccacgc cgcgcacagt cgccatggat tgcgtatcga
     1081 ccagagtaac tgctgatatc ggttcgccat taagttcacg ctgttcaatg aatggcagct
     1141 cgtgtccata taaggtcagg aaggcgcgca cattccggtt catccgccac ggaagaactg
     1201 gggtcgaatc tggatcggta agatgcgctg ccagcagaga tgccagggcg tcgaaatcag
     1261 cttgctcgtg agtcagtata attcgcatag gctaataatg gatcttctct tcgaataatt
     1321 gtaaacgatt gtaatggaat ttggtgccct ggtcagagcg tttgcgggat gaggtaataa
     1381 atgaacccca accacaagtc atccatttcg gtgattgacc agtcggagtg gactcactat
     1441 aatggctgct atgaacctga acgatttaac catcgcgatg catgaatttg tacgctccca
     1501 gggttggtac gaagccggca ccagacggcc tcagactctc cgcaacctgg ctatatcact
     1561 caatttggaa gccagtgaag tcctggaaca ttttcaatgg cgtgagaacg ctgaccatcc
     1621 ggacgagctg ggttccgagc tggcagatgt ggctctgtat ttactgcaaa ttgccagtat
     1681 cgctgggatc gatctggaac aggcgatatt gaaaaaattg gaagtcaacc agctacggaa
     1741 gtgggatcaa gaagaatgag caacataacc gtattcggcg gatcccaaat ccagcccgga
     1801 atgcctgatt acctggctgc ccagcgcctt ggaggtctcc tggcgatggc aggccactgt
     1861 gtgctcaccg gcgggtacat cggtgctatg gaggctgtct cacgcggcgc tgctgaggct
     1921 ggtggacatg tgatcggagt gacctgtgcg gaaatcgaag cctggcgacc gggtaaagcc
     1981 aacgcctggg ttaaagagga gcgttgtttc ccc
//
LOCUS       DATMXK010000332         2079 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_70391, whole genome shotgun sequence.
ACCESSION   DATMXK010000332 DATMXK010000000
VERSION     DATMXK010000332.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2079)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2079)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2079
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70391"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..927)
                     /locus_tag="VN363_06140"
     CDS             complement(<1..927)
                     /locus_tag="VN363_06140"
                     /inference="COORDINATES: protein motif:HMM:NF013727.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FtsK/SpoIIIE domain-containing protein"
                     /protein_id="HWQ84127.1"
                     /translation="MIPKIDSQAIQRLQEVILQTFNQAGLPVQIIQTIPGPWFCQLTI
                     EAAPGAGQGFLGRFRPKKFAIQKTILDQTNFYLMQIDNPIPRRPRLKILALNRDIPSI
                     PLQPAVTPPTYQQITGNLIFPLGVDLSGEPVSADLTTFPHLLVGGTTGGGKSVFVNSL
                     LAGLLCRYSPDELRLVLCDGNTVELSPYQGIPHLAGKVATQSKEILPILDWLQAEVQQ
                     RLEAISSVRQELPYLLVVLEHTFIFTRADPRVSSLLTVLLPLAPRARVHFIFTASYPD
                     AKVLPTAVKRQIPARVCFSVVDPEASRMVINQS"
     gene            complement(1341..>2079)
                     /locus_tag="VN363_06145"
     CDS             complement(1341..>2079)
                     /locus_tag="VN363_06145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004488 - methylenetetrahydrofolate
                     dehydrogenase (NADP+) activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetrahydrofolate dehydrogenase/cyclohydrolase
                     catalytic domain-containing protein"
                     /protein_id="HWQ84128.1"
                     /translation="AAYVASKQKACAELGMGSVSQHLPGDASQQQVEDLVKGLNDDPK
                     INGILVQLPMPGHIDEERVLQLINIEKDVDGFSPLNIGRLAQKGREPLFVPCTPYGCI
                     YLLEQSGVKIEGANAVVLGRSNIVGMPAALLLIGRNATVTVCHSRTRDLPSVVRQADI
                     LIAAIGRTEMVRGDWIKPGAAVIDVGINEIADPTKKSGRRLVGDVNFAEAVEVAGCIT
                     PVPGGVGPMTIAMLMKNTLHAAEIQNP"
BASE COUNT          522 a          492 c          576 g          489 t
ORIGIN      
        1 gctttgattg atgaccatgc gtgaagcctc ggggtcaacc accgaaaaac atacccgtgc
       61 cgggatctgc cgtttaaccg cggtgggcag gacttttgca tcgggataag aagcggtaaa
      121 aatgaaatgc accctggccc ggggagccag ggggaggagc acggtaagca gactggatac
      181 acgcgggtca gcccgggtaa agataaatgt gtgctcaagt actacaagca gataaggcag
      241 ttcctgtcta accgatgaga tagcttctaa tcgctgttgt acttcagctt gcaaccaatc
      301 caggataggc aggatttctt tggactgcgt tgctactttc ccagcgagat gagggatacc
      361 ctgatacgga cttagctcaa cagtatttcc atcacataac acgagacgca gttcatctgg
      421 cgaataccgg cagagcaagc cagccagcag actgttcaca aacaccgatt ttccgccccc
      481 agtggttcct cccaccaaaa gatgtgggaa ggttgtcagg tctgctgata caggttcgcc
      541 ggataaatct acacccaatg gaaaaatcaa attgcctgtg atttgctgat aggttggagg
      601 ggttaccgcg ggttgcaggg gtatggatgg gatatctcga tttaacgcca ggatctttaa
      661 tcgagggcgg cggggtatgg gattgtcgat ctgcatcagg tagaaattgg tttgatccag
      721 aatggttttt tggatagcaa atttttttgg gcgaaagcga cccaggaatc cttgaccggc
      781 gccaggtgcg gcttcgatcg ttaactggca gaaccagggc ccgggaatcg tctggataat
      841 ttgcacgggc agaccggcct ggttaaaggt ttgtaaaatg acttcctgta aacgctgtat
      901 agcctgggaa tctattttgg gaatcacgct gcgatccaga atccccgtgg ggggcggaac
      961 acgccagtga gcgtttgatg cggttaaggg atgggaagga agcaacgagt tggatgacat
     1021 acagatcacc atcaccggtt tgctgtaaaa agtccatcga atggttagtt gttgcgtgaa
     1081 tctattatag tctcatatat tcggaattga aattgagttg aagtaggcgt acgaaacaaa
     1141 aaattgatct tgaaatcaag attattcagg ttataattga ccacaataga acacatgttt
     1201 tgtctctgaa aattttctgg aagcctgtat gagatgtgct ggatgagagt tggataactc
     1261 gccagcaagt tgaaaaatca gaacataagc aacgagccgg tgttatccac cggcccgtga
     1321 ctgagctaaa cagcttagtt ttacggattt tgaatttcgg cggcgtggag ggtattcttc
     1381 atcagcatag caatggtcat cgggccaacc ccgcccggta caggtgtgat gcagccggcg
     1441 acttcaacag cctcggcaaa gttcacatcg cctaccaggc ggcgcccact ctttttcgtt
     1501 ggatccgcga tttcattaat cccaacgtcg ataaccgctg cgccaggttt gatccagtcg
     1561 ccgcgcacca tctcggtgcg accgatcgcc gcgatcagga tatcagcctg gcgaaccaca
     1621 ctcggcaggt cacgcgtgcg ggaatggcaa acggtgaccg tggcattgcg cccgatgagc
     1681 aagagggcgg ctggcattcc gacgatgttt gagcgaccga ggacaactgc gttggcgcct
     1741 tcgatcttca ccccggattg ttcgagcagg tagatgcagc cataaggagt gcagggcacg
     1801 aagagcggct cgcggccttt ttgggccaga cgtccaatat taagcggcga gaaaccatcg
     1861 acatcttttt cgatattaat cagctgcaaa acgcgttctt catcaatgtg gccgggcata
     1921 ggcagctgga ccagtatgcc attgatttta ggatcatcat tcaggccttt taccaggtct
     1981 tctacctgct gctggctggc gtcaccaggc aaatgctggc tgactgagcc catgcccagc
     2041 tcggcgcaag ccttttgctt ggaagccaca taagcagca
//
LOCUS       DATMXK010000333         6780 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_54740, whole genome shotgun sequence.
ACCESSION   DATMXK010000333 DATMXK010000000
VERSION     DATMXK010000333.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6780)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6780)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6780
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54740"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..248)
                     /locus_tag="VN363_06150"
     CDS             complement(<1..248)
                     /locus_tag="VN363_06150"
                     /inference="COORDINATES: protein motif:HMM:NF013080.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008484 - sulfuric ester hydrolase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfatase-like hydrolase/transferase"
                     /protein_id="HWQ84129.1"
                     /translation="MSDSPTQPNLLFLISDQQRADTLAPGSACYTPNLDRLAQRGVRF
                     DSCYAPNPICAPVRASLFTGLLPHNHGMVDNPHTVESYR"
     gene            complement(268..1350)
                     /locus_tag="VN363_06155"
     CDS             complement(268..1350)
                     /locus_tag="VN363_06155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWQ84130.1"
                     /translation="MSDLIEPTGIAQPPSGKSSLPRKFSSNPPLLEVRNLKKYYPIHK
                     GWMRKVVNYVRAVDEVSFFVREGETLGLVGESGCGKTTTSRCILRAFPVTSGEILFRE
                     ADGKVTDLAKLDRPGLRRFRPQMQMIFQDPFGSLNPRMTIFDIVAEPMYVNGLTNRSE
                     RTDRVAELLKLVGLRQEYMRRYPHAFSGGQRQRIGIARALALNPRLVVADEPVSALDV
                     SVQAQVLNLMLDLQDKLQLTYLFVAHDLSVVKHICDRVCVMYVGKIAETASTEELFHA
                     PLHPYTAALMMAVPVPDPSRRHIIQDLPGEVASPANPPSGCYFHPRCEYAQERCRIEP
                     PQLREIRPDHFVSCHRAEELHLAGIK"
     gene            complement(1347..2474)
                     /locus_tag="VN363_06160"
     CDS             complement(1347..2474)
                     /locus_tag="VN363_06160"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84131.1"
                     /translation="MSNERGHSKQFTGQVPASPLDLKDQPLLRVEDLKAYFFMDEGTV
                     KAVDGVSFNVFPGKVVGIVGESGCGKSVTMKAILRILEPSAKIVSGKVTLRSRLNSAD
                     PDSPEELIELTQLHPNSKILRKIRGGELALIPQEPMAAFSPVHTVGSQIIETILLHQD
                     VSKADARQLAISVFRAVGISMPEQRIDAYSWELSGGLRQRAIIAMALSCNPRLLIADE
                     PTTAIDVTTQAQILKLLRTLQEERQTAIIFITHDLGVIAQMADEVVVMYLGMVMEKGP
                     VNAIFKNAKHPYTQALLRSIPSVQSESRVKLPTIAGSIAHPFNRPAGCPFHPRCIHFM
                     RGVCDASQPQQVQIGEEHYVSCFLYPPEVTSPVGPLAEKTS"
     gene            complement(2492..3721)
                     /locus_tag="VN363_06165"
     CDS             complement(2492..3721)
                     /locus_tag="VN363_06165"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84132.1"
                     /translation="MDSNQSTEFGSQATPDIAPADDIDAVVPTSSIEETAPPAESLVV
                     GQDEERISIASNWTLVWWRFRKNKLAVISGIILIFMLIVVLVPDFFATMDPEDTDARL
                     AFIPVQTIHWMDEGRLKPWVPGVIGARDPVTLKMIWTVDENVKIYIRLFGKGDPYKIL
                     GLVGSDIHLASAVDPENSGKIFLLGTDRLGRDQWSRLAFASRISMTIGMVSVAVSIVL
                     GLILGGISGYFGGLPDLVIQRLIELLQSLPTIPIWLALTAALPRTWSPERVFFAITII
                     LALRGWTTLAREVRGRFLSLREEDFVLAAELAGASQWRIILIHMIPTFTSHIIATSTL
                     AIPVMIVTETSLSFLGLGLRPPAISWGVMLQEAQNIQTLALAPWLLFPGLVVIVAVLV
                     FNLVGDGLRDAADPYGN"
     gene            complement(3724..4713)
                     /locus_tag="VN363_06170"
     CDS             complement(3724..4713)
                     /locus_tag="VN363_06170"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84133.1"
                     /translation="MIRYILQRLLLIPVLLVLFSVMVFALVQAPPGDYLTTYIATLSA
                     SGSSMDQAQVDALEKQFGLDQPVHIQYVRWMQSLARGELGLSLEYQRPNAELIGERLG
                     LTIVLALAAFVFTWVVAIPIGIYSATHRNSILDYLITVINYTGVAIPNFMLALILMWL
                     AYSYFDISITGLFSREFEQAPWSFARVIDLMKHIWLPMIVIGIAGTARLTRVMRANLI
                     DELNKPYMVSGRARGLSEWQLVLKYPVRLAINPLASTIGWYLPELFSGSLIVATVMNL
                     QNIGPLLLRSLTNQDMYLAGSILLIYCFLAIIGTLLSDILLAWVDPRIRMEKS"
     gene            complement(4841..>6780)
                     /locus_tag="VN363_06175"
     CDS             complement(4841..>6780)
                     /locus_tag="VN363_06175"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ84134.1"
                     /translation="TEAPAEPVATEAPAEPVATEAPAEPVATEAPAMTYNESPLLKAM
                     VDAGELPPVEERLPENPLVIQVTDSIGQYGGTWHRGFLGPSDYNNYVRVVYDGLVRFS
                     PDGSKVEPRLIESWESSEDFTTWTIHMRKGAKWSDGEPFTADDIMFWYEDILLNPDLT
                     PSLPKWMTNADGTPVVVTKVDDYTVTWNYASANTTLMMELANQDGGDKFYAVFQPAHY
                     LKQFHAKYANADELNKMVTDASFNTWPELFATRKNPAENPDRPVMAAWQPASRVSDEV
                     LVLKRNPYFVGVDAEGNQLPYIDEIQFKFFQDTQALNLAAIAGELDLQERHMIMGNYP
                     VLKQNELDGKYRVITWPTFGGSDAAITFNQTYNKDPIVAELLQSKDFRVALSHAMDRE
                     QIKESVFLGLGEARQPVPAPWHPYYPGDEAAFKYTEYDPDLANELLDGLGLTERDGEG
                     FRLNKDGQPFSIEISVVPNFANWPDVAQMVAADWQDVGVRTIVQIRERAVHFEMREAN
                     EIVTELWNEDTTGFPFTGAPKFDPRSQPGLGIAPLMRQWLQTSGAEGVEPTDAFKRIV
                     EIIDTGKTVGTEEQIALAQELFTLWTDECYEIGTVGLTPLVQGVVVLNNSLKNVPETL
                     GNDWPLRTPGNAVPEQFYFEQ"
BASE COUNT         1624 a         1745 c         1953 g         1458 t
ORIGIN      
        1 cggtaagatt cgacggtatg gggattatcg accatgccgt gattgtgcgg gagcagaccg
       61 gtgaacaggc tggcgcgcac cggggcgcaa attggattgg gggcgtaaca gctatcaaac
      121 cgaacgccgc gctgggccag gcggtccaga tttggtgtat aacaggctga gccgggggca
      181 agggtatcgg cgcgctgctg gtcagagatc aagaatagaa ggtttggttg agttggtgaa
      241 tccgacatga tgttgggggt gaaacctcta tttaatcccg gccagatgaa gctcttcggc
      301 gcgatgacag gaaacaaaat gatccggtct gatttcacgc agctggggcg gttctatgcg
      361 gcatctctcc tgggcgtact cacaccgggg atggaaataa cagccactgg gtggattggc
      421 cgggctggca acttcgcccg gcaggtcctg gatgatatgg cgccgggagg gatcggggac
      481 tggcactgcc atcattaagg cggcggtata ggggtggagg ggtgcatgga agagttcttc
      541 ggtcgaagcc gtctcagcaa tcttgccaac atacatcacg caaacccggt cacaaatatg
      601 cttgaccaca ctcaaatcat gggctacaaa caggtaggtc agctgcagct tatcctgcag
      661 atccagcatc aagttaagca cctgggcctg aacggagacg tccagagctg aaacaggctc
      721 atcggcgacg accaggcggg gatttaatgc cagggcgcgg gcaatgccaa ttcgctggcg
      781 ctgaccgccg ctgaaggcat gggggtagcg gcgcatgtac tcctgacgca agccgactaa
      841 cttgagcaat tctgcgacgc gatcggtgcg ctcggagcgg ttggtgagac cattgacgta
      901 catcggctca gcgacaatat caaaaatggt catccgtgga ttaagcgatc caaacggatc
      961 ctgaaagatc atctgcatct ggggacggaa gcgcctcaat cctgggcgat ccaattttgc
     1021 caggtcagtc accttgccat ccgcttctct gaagaggatt tccccgctgg tcaccggaaa
     1081 agccctcaat atacagcgtg aggtggtcgt tttaccacag ccgctttccc caaccaggcc
     1141 cagggtttcg ccctcgcgca cgaaaaaact gacctcatca acggcgcgca cgtaattgac
     1201 cactttgcgc atccagcctt tatggatcgg gtaatatttt ttcaggttgc ggacttcaag
     1261 caggggcggg tttgaactaa atttccgggg aagggaggat ttcccagaag gtggttgggc
     1321 aatacctgtg ggttcaatta agtcgctcat gaggttttct ccgcgagggg accaacgggt
     1381 gaggtgactt ctggcggata caggaagcag ctgacataat gctcttcccc gatttgcacc
     1441 tgctggggtt gggaggcatc gcaaacaccg cgcataaagt gaatgcagcg agggtgaaat
     1501 ggacaaccgg cgggtcgatt gaaaggatgg gcaattgagc cggctatggt cggcagcttg
     1561 acgcgcgatt ccgactgcac gctggggatt gaacgaagta aggcctgagt ataaggatgc
     1621 ttcgcattct tgaagatcgc attgaccggg cctttttcca tcaccatacc cagatacatc
     1681 acaacaactt catctgccat ctgggcaata acacccagat cgtgggtgat aaagatgatc
     1741 gcagtctggc gttcttcctg cagggtgcgc agcaatttca agatctgagc ctgggtggtg
     1801 acgtcgattg cggtggtggg ttcatcggcg atcaacaggc gcgggttaca actcagcgcc
     1861 atggcgataa ttgcccgctg gcgaagcccg ccagaaagct cccacgaata agcgtcgatg
     1921 cgttgttccg gcatggaaat acctacggcg cggaagacgc tgatagcgag ctggcgcgcg
     1981 tctgccttgc tgacatcttg atgaagcagg atggtttcta taatctggct gccgacggta
     2041 tgcaccgggc tgaaggctgc catcggctcc tgggggatga gggcaagctc accgccgcgg
     2101 atcttgcgca ggattttgct gtttgggtgc agctgggtca gctcgatcaa ctcttccggg
     2161 ctgtccggat ccgcactgtt taagcgactg cgcagggtaa cttttccgga gacaattttt
     2221 gctgaaggct ccaggatgcg taagatggct ttcatggtga cgcttttgcc acagccgctt
     2281 tcgccgacga tcccgaccac cttgcccgga aaaacattaa agctcacgcc atcgaccgct
     2341 tttaccgtgc cttcatccat gaagaaataa gctttgagat cctcaaccct gagcaaaggt
     2401 tggtctttca gatcgagagg ggaggctggg acctgacctg tgaactgctt agagtgtccg
     2461 cgttcatttg acatgcgtta atcctcccag attagttgcc atacgggtca gcggcatcgc
     2521 gcaagccatc acccaccagg ttgaacacca atacggctac gattaccacc agccccggaa
     2581 aaagcagcca gggcgccagc gccagagtct gaatattctg agcttcctga agcatcacgc
     2641 cccaactgat agccggtgga cgcaaaccca ggcctaagaa gctcagtgaa gtctcggtga
     2701 cgatcatgac cgggatagcg agggtactgg tggcgatgat atggctggtg aaggtaggaa
     2761 tcatatggat gaggatgatg cgccactggc tagcgccagc caattcggct gccaggacga
     2821 aatcttcctc ccgcaacgat aagaagcggc cgcgcacttc acgcgccagg gtcgtccagc
     2881 cgcgtaaagc cagaatgatg gtgatggcaa aaaatactct ttcgggcgac caggtgcgcg
     2941 gcagagctgc ggtcaaagcc agccagatcg ggattgtggg cagggattga agtaattcaa
     3001 tcaacctctg gatgaccaga tcgggcaagc ccccaaaata cccggagatg ccacccagga
     3061 tcaatcccag cacgatgcta acggccaccg aaaccatacc gatcgtcata gaaatccgcg
     3121 aggcaaaggc cagacgtgac cactgatcgc gtcccaaacg atctgttcca aggagaaata
     3181 tttttccaga gttttccggg tcgacagcgc ttgccagatg aatatccgat ccgaccaggc
     3241 ccaggatttt atatgggtcg ccttttccga acaaccggat atagattttt acgttttcat
     3301 ccacagtcca gatcatttta agggttaccg gatcccgtgc gcctataacg cccggaaccc
     3361 agggcttcag acgaccttca tccatccagt ggatcgtttg aaccggaata aatgccagcc
     3421 gggcatccgt atcttccggg tccatggtcg caaagaaatc cgggacgagg acgacgatca
     3481 acatgaagat gaggataatc ccgctgatca ctgccagctt gtttttgcgg aaacgccacc
     3541 agacgagagt ccagttggag gcgatggaga tgcgttcttc atcctggcct accaccaggc
     3601 tttcagcagg tggggcagtt tcctcaatgg atgaggtagg gacaacggca tcgatatcat
     3661 ctgcaggcgc aatatcgggg gtcgcttgcg aaccgaattc tgtagattgg ttgctgtcca
     3721 tattcagctc ttttccattc ggatgcgagg atcgacccag gccagtaaga tatccgagag
     3781 caatgtgccg ataatcgcca ggaaacagta aatcaacagg atactgcctg ccaggtacat
     3841 atcctggtta gtcaacgaac gcagcagcaa aggaccaata ttttgcagat tcatcacagt
     3901 agcaacgatg aggcttccag agaataactc gggtaggtac cagccgatgg tgcttgccag
     3961 cgggttaatc gccaggcgga cggggtattt cagcaccagc tgccattctg aaagcccgcg
     4021 cgcccgtcca gaaaccatat aaggcttgtt gagctcatcg atcaggttgg cgcgcatcac
     4081 ccgggtcagc cgcgccgtac cggcaatccc aatcacgatc atcggcagcc agatatgttt
     4141 cattaaatct atcactctgg caaatgacca gggcgcctgt tcgaattccc tggagaataa
     4201 gccggtgatg ctgatgtcaa aatatgaata tgccagccac atcaagatca atgccagcat
     4261 aaagttcgga atggcgacac ctgtgtaatt gatgaccgta atcaggtaat ccaggatgga
     4321 attacggtgg gtggcagaat agataccgat tggaatggcg acaacccagg tgaagacgaa
     4381 ggcagccaaa gccaaaacaa tggtcagacc caggcgctca ccaattaact cggcatttgg
     4441 acgttgatat tcgagggata atcccaactc accgcgggcc aggctttgca tccagcgcac
     4501 atactgaatg tggactggtt ggtccaggcc aaattgtttc tcgagagcat cgacctgggc
     4561 ctgatccatg gatgagccag aggcggacag ggtggcaata taggtggtca ggtaatctcc
     4621 gggaggagcc tggaccaacg caaaaaccat gaccgagaag agaactagca ggacggggat
     4681 caacaataac cgctgaagaa tgtagcgtat cattggagta cagcctatcc ttcctggctc
     4741 aagtgggagg cttgaccgcg gccagacaag actggcctcg ataagtcatc ctgcgagctc
     4801 aaagtagagc tcgcaggatg ttgggtctaa agttcaagat ttactgttcg aaatagaatt
     4861 gttccggaac ggcattgccc ggtgtgcgta acggccagtc attgccgagg gtctcaggaa
     4921 catttttgag actgttgttg agtaccacaa caccctgaac caggggagtg aggccaacag
     4981 taccgatctc atagcattca tccgtccaca aggtaaagag ctcctgagcc agcgcaattt
     5041 gctcttcggt accgactgtc ttgccggtat cgataatttc gacgatgcgt ttgaaagcat
     5101 cggttggctc aacgccctca gccccactgg tctgcagcca ttggcgcatc aaaggtgcga
     5161 tgcccaggcc aggttggctg cggggatcaa atttcggagc gccagtgaag gggaagccag
     5221 tcgtgtcttc gttccacagc tcggtgacga tttcattggc ttcacgcatc tcaaagtgta
     5281 ccgctcgctc acggatctga acgatcgtgc gcacaccaac atcctgccaa tcggcagcca
     5341 ccatctgggc aacatcgggc cagttggcga agtttggcac aaccgagatt tcgatactga
     5401 agggctggcc atccttgttc aggcggaagc cttcgccatc gcgttcggtc aggccgagac
     5461 catccaggag ctcattggcc agatcggggt catactcggt gtatttaaag gcggcttcat
     5521 ctccaggata gtagggatgc cagggagccg gcaccggctg gcgggcttca ccgagaccaa
     5581 ggaagacaga ctctttgatc tgctcgcgat ccatcgcatg agacagagca actcggaaat
     5641 ccttggattg gagcagctcg gcaactatgg gatccttgtt gtaggtctgg ttgaaggtga
     5701 tcgccgcatc ggaaccgccg aatgtgggcc aggtgataac acgatattta ccatccagct
     5761 cattttgctt aagcactgga tagttgccca tgatcatgtg gcgctcttgc aaatccagct
     5821 caccggcgat cgcggccagg ttcagggcct gggtatcctg gaagaacttg aattggattt
     5881 catcgatgta gggcagctgg ttgccttctg catcgacacc cacaaagtag ggattgcgtt
     5941 tgaggaccag gacttcgtca ctgacgcgcg aggcaggctg ccaggcggcc atcacaggcc
     6001 ggtcgggatt ctcagccgga ttcttgcggg ttgcgaagag ctcaggccag gtgttaaagc
     6061 ttgcatcggt aaccatcttg ttgagctcat cggcgttcgc atacttggca tggaactgct
     6121 tcaggtagtg ggcaggttgg aagaccgcgt agaatttatc gccgccatcc tggttggcta
     6181 attccatcat cagggtggta ttggcggagg catagttcca ggtgaccgta taatcgtcca
     6241 ctttggtgac aaccaccggt gttccatcgg cgttggtcat ccatttgggc aagctggggg
     6301 tcaaatctgg attgagcagg atatcttcgt accagaacat aatatcgtct gctgtgaaag
     6361 gctccccatc cgaccatttg gcgcctttac gcatgtgaat ggtccacgtg gtgaaatctt
     6421 cagacgattc ccaggactca atgaggcggg gttcaacttt actgccatct ggagagaagc
     6481 gaaccaggcc atcataaacc acacggacat agttgttata gtcggatggt ccaagaaagc
     6541 cacggtgcca ggtgccaccg tattgaccga tggaatcggt gacctggatc acgagggggt
     6601 tttcggggag gcgttcttca acaggaggaa gctcacctgc atcgaccatc gctttcagca
     6661 ggggagattc gttgtaggtc atggcaggag cttcagtagc gaccggctct gcaggagcct
     6721 cagtagcaac tggctcagca ggcgcttcgg tagcaaccgg ttctgcggga gcttcagtgg
//
LOCUS       DATMXK010000334         2568 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_96888, whole genome shotgun sequence.
ACCESSION   DATMXK010000334 DATMXK010000000
VERSION     DATMXK010000334.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2568)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2568)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2568
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_96888"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            47..1960
                     /locus_tag="VN363_06180"
     CDS             47..1960
                     /locus_tag="VN363_06180"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HWQ84135.1"
                     /translation="MAAYTHFRGFDLTPRIAKIERTFRCEPLTTAEDFPILINAPCYF
                     SFGSRDKPHDYYTNPASMLAYQAKGYETHLQRVQDDVVPYFMPWFGTGVLASAFGAPV
                     HLPDNPADDPAVLEACLKSPGDIARLKLPDPERDGWMPRVLKTIDYAVTYGDLPVGLT
                     DMQGPLDTVGMLCGQAQLYQWMYSEPAMIHELFDIVTEAFIQWVKVQKQHIGEPLERS
                     NGLQGAFSPGCGVWESDDDLVLIDSNLYGEFVAPHLSRIFDVFGGGSIHFCGGAVQHL
                     DTFRTVRNLRVINNSPLGNFAAFSRLAQSLAGKITLQIQDAAPVDVEGYYANLFAGID
                     DFRGMMLASFVLDNAGMDGAGGYVPVDWDAFETSNRIVASIRDSIARRLVGEDTRPVT
                     PEAAPITVKDGSSASVGMSTPPEFNPQQQAALERVRESLIEFEQEEVKLAVQAALDLK
                     ILPFDIILRGLALGMQEVGRLYELGDYFLPQLVMAGATMQAAMDVLQPSLQGAGGAAA
                     ISKGKVILGTVKGDLHNIGKNLVRTMLEGASFEVIDLGVDVPPQKFVEAALAHGAQLV
                     ALSALLTTTLQGMQQTVAALQAAGLAGRVHVMIGGAPVSREYADRISAEGYAASAVGA
                     IAEAERLLGLGQA"
     gene            1994..>2568
                     /locus_tag="VN363_06185"
     CDS             1994..>2568
                     /locus_tag="VN363_06185"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84136.1"
                     /translation="MSIQIFPSTYKDQRALTLESELLRAQFLPEIGAKMASLIYKPRN
                     FELLVQRPGEKYLIQPFDGVFTDGECSGFDDMFPTIDTCFYDRYPWEGIKMADHGEVW
                     SLPHTFESEGDRLHFKVNGIRFPYCLEKWISFTAPNILHLDYQLTNLTPFDFDFVWAG
                     HTMFNLEEGVELSLPEGVRKITHTFGIGAEIA"
BASE COUNT          563 a          653 c          726 g          626 t
ORIGIN      
        1 gccatacaca cccgccttag gcgagcgtta gcccgaggcg cacccattgg ctgcatatac
       61 acattttcgc gggttcgatc tgacccctcg gatcgccaag attgaacgca ccttccggtg
      121 tgagccgctg actaccgccg aagatttccc tatcctgatt aatgccccct gttatttctc
      181 cttcggtagc cgcgataaac ctcatgacta ttacaccaac cctgccagca tgttggctta
      241 tcaggctaag ggctatgaga cccacctgca gcgcgtgcag gatgatgtcg taccctattt
      301 catgccctgg tttggcacag gcgtcctggc ttcggccttt ggtgcaccgg tgcatctgcc
      361 ggataatcct gccgatgatc ccgctgtcct ggaagcatgt ctcaaaagcc caggcgacat
      421 tgcccgctta aaactgcctg atccagaaag ggatggctgg atgccgcgcg tcttgaaaac
      481 catcgactat gcggtaacct atggagattt gccggttggg ctcaccgaca tgcaaggtcc
      541 gttggatacg gtgggtatgc tatgtggtca ggcgcagctt tatcagtgga tgtacagcga
      601 gccggctatg atccatgagt tgttcgatat cgtcaccgaa gcctttatcc agtgggtgaa
      661 ggtgcagaaa cagcatattg gtgagccatt ggagcgcagc aatggcctac aaggtgcctt
      721 ttcaccaggt tgtggagtat gggaatcgga tgatgacctg gtgttgatcg attccaacct
      781 gtatggggaa tttgtcgctc ctcacctgtc acgcatattc gatgtatttg gcggcggtag
      841 catccatttt tgtggcggcg ccgtccagca tctggatacg tttcggacag tgcgtaacct
      901 gcgcgtgatc aataactcgc ccctgggcaa ctttgcggct ttcagtcgcc tcgcgcaaag
      961 cctggctgga aaaatcaccc tgcagatcca ggacgctgcc ccggtggatg tcgaaggtta
     1021 ctatgctaac ctgttcgctg gtattgatga cttccgtggg atgatgctgg ctagcttcgt
     1081 gctggataat gcgggcatgg atggggcggg agggtatgta ccggtcgatt gggatgcatt
     1141 tgaaacatct aatcgtattg tggccagtat ccgggattct attgcccggc gcctggttgg
     1201 tgaggatacc cggccagtaa cgccagaagc tgcacccata accgtaaagg acgggagcag
     1261 cgcgtccgtc gggatgagta ctcctccgga atttaacccc cagcagcaag ctgctctgga
     1321 gcgggtccgg gaaagcctga ttgaatttga acaggaagaa gtcaaactgg cggtgcaggc
     1381 agcgctagat ttgaaaatcc tgccttttga tatcatcctc cgaggtctgg ctctaggtat
     1441 gcaggaggta ggccgcctgt acgagctggg tgattatttc ctgccccaac tggttatggc
     1501 tggcgctacc atgcaggcgg caatggacgt gctgcagccc agcttgcaag gcgccggcgg
     1561 tgcagcggcg atctccaaag gcaaggtgat cctgggcacc gtcaaaggtg acttgcacaa
     1621 tattggcaag aacctggtgc gaaccatgct ggaaggcgcc agttttgagg tgatcgacct
     1681 gggtgtggat gtgccacctc agaaatttgt ggaagccgct ctggctcacg gtgcacagtt
     1741 ggtagcactt tctgccctgc tgaccaccac attacaggga atgcagcaga ccgtggcagc
     1801 tttgcaagct gctggactgg ccgggcgtgt acatgtgatg attggtgggg cacccgttag
     1861 ccgggagtat gccgaccgga ttagcgctga aggatatgca gccagcgcag taggtgcgat
     1921 tgctgaagct gaacgcctgc tcggattggg acaggcttga aagttttcat ctttctataa
     1981 tcaggagagc cacttgtcaa tccaaatttt tccttccacc tataaagatc aacgtgcgct
     2041 gactctcgaa agcgagctcc tgcgggccca attcttaccc gagatcgggg cgaaaatggc
     2101 ttctctgatt tacaaaccgc gcaattttga attactggtt cagagaccag gggaaaaata
     2161 tctaatccag ccgttcgatg gggtctttac cgacggtgaa tgctccggat tcgatgatat
     2221 gtttcccacc atcgatacct gtttctatga tcgttatccc tgggaaggta tcaagatggc
     2281 cgaccacggt gaagtgtgga gcttaccgca caccttcgaa tcggaaggtg acaggcttca
     2341 cttcaaggta aatggaattc gattcccata ctgcctggaa aaatggatct ctttcactgc
     2401 gccgaacatt ttacatctgg actaccagct gaccaacctt accccatttg attttgactt
     2461 tgtgtgggcc gggcatacga tgttcaatct ggaagaagga gttgaacttt ccttaccgga
     2521 gggtgtgcgg aaaattacgc atacatttgg gatcggcgcg gaaattgc
//
LOCUS       DATMXK010000335         2393 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_153573, whole genome shotgun sequence.
ACCESSION   DATMXK010000335 DATMXK010000000
VERSION     DATMXK010000335.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2393)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2393)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2393
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153573"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..590
                     /locus_tag="VN363_06190"
     CDS             <1..590
                     /locus_tag="VN363_06190"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWQ84137.1"
                     /translation="WGAHVAAGCPDAQLALGQVLQPDQRLELMENLGFYRAPEIGLET
                     FSGTRPASWPEGEAELLGTGELVISPLQMARAAAALSSGGNLPSLSLATGVSTAAGGW
                     ELFPAEASAGSGLPAGGAQRAARLLALPDQDLWEATSLAPMGEEQQAAWYLAGSLPGS
                     ATPRVVVVLLEDANARSAQQAGRALLSAALQIGIP"
     gene            complement(621..1853)
                     /gene="tyrS"
                     /locus_tag="VN363_06195"
     CDS             complement(621..1853)
                     /gene="tyrS"
                     /locus_tag="VN363_06195"
                     /EC_number="6.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR00234.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004831 - tyrosine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006437 - tyrosyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tyrosine--tRNA ligase"
                     /protein_id="HWQ84138.1"
                     /translation="MEIRQMTIDQQVAYLMQGTEYGDEALKQAMADELRTRLLEAQKE
                     NRPLRVYCGFDPRTSDLHLGHTVPMRKLRQFQELGHQVIFLVGTYTSLIGDPSDKDKL
                     RPQLTPEQVVANARTYAQQAFKVLDESKTEVRFNAEWLSELSFAELIGLASNFTLQQF
                     LTRDNFRLRWDKGDPIYLHETFYSIMQGYDAHALKADVQVGGTDQLFNIVTAARKLMT
                     YLDGRPNIAIIVGILPGTDGDVKMSKSLGNHIPLLAEPGDMYGKVMSIPDKAMGAFFR
                     LVTRWTSDEITALENGMAAGTHNPRDVKMKLAKEIVAIYHSEAAAAQAESEFVRVFQQ
                     HNLPAEIPDFCLQAGQSVLDVLVAGGLVPSRGEGKRMLQQRAVRLDGETLQEADAEFP
                     HPGVLQVGKRRYLRVLGS"
     gene            complement(1935..>2393)
                     /locus_tag="VN363_06200"
     CDS             complement(1935..>2393)
                     /locus_tag="VN363_06200"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84139.1"
                     /translation="GLAWLAALGLGAAAGALRGQGGAVIGSLPYLAGVVALMAASISL
                     SNWMDRQTVLRLDPRGMFFTNGLRRVSLPWDQIDQVTVFSGSLGKRVQVVGGVQHFEF
                     RYLSEVEVLGHPQGRLGFPDGVKILAEILRETNLQRSPNSQGDTVYYSRN"
BASE COUNT          478 a          743 c          726 g          446 t
ORIGIN      
        1 cctggggggc gcatgtggca gccggctgcc cggacgccca gctggcgtta gggcaggttt
       61 tacagcccga ccaacgcctg gaattaatgg aaaacctggg attctaccgc gcccccgaaa
      121 tcggcctgga gacgttcagc ggcacccgcc cggcaagctg gccggaaggt gaggctgagc
      181 tgctcgggac aggcgagctg gtgatcagtc cacttcagat ggcccgcgct gcagccgccc
      241 tgagcagcgg tggaaactta ccctcgctaa gcctggcaac cggggtgagc acggccgcgg
      301 gcggatggga gctgttcccc gcagaagcct cggccgggag cggccttccc gccggcgggg
      361 cccagcgggc ggcgcgcctg ttggcgttac cggatcagga tctctgggaa gcgaccagcc
      421 tggctcccat gggtgaagaa cagcaggcag cctggtatct ggcaggcagt ctgcccggca
      481 gcgccacgcc gcgggttgtt gtcgttctgc tggaagatgc aaatgccaga agcgcccaac
      541 aggccggaag ggctttgctg agcgctgcac tgcagatcgg gatcccgtaa gatcccgttt
      601 catcttttag aattagttaa tcagctccca agcacacgca aataacggcg cttgcccacc
      661 tgcagcacgc caggatgggg aaactcggca tccgcttcct ggagggtctc cccatccagg
      721 cgcacggcgc gctgctgaag catgcgcttg ccctcgccgc ggctgggcac cagcccacct
      781 gctaccagca catccagcac gctctgcccg gcctggaggc agaaatccgg gatctcggcc
      841 ggcagattgt gctgctggaa aacgcgcacg aactcggatt cagcctgtgc agcggcagct
      901 tctgaatggt aaatggcgac gatctccttc gccagcttca tcttgacatc ccgcggattg
      961 tgggtcccgg cagccatgcc attttccaat gcggtgatct catccgatgt ccagcgcgtg
     1021 accagccgga agaatgcgcc catcgcctta tccgggatgg acatcacctt gccgtacata
     1081 tctcctggtt cggccagcaa ggggatatgg ttgcccagcg atttactcat tttgacatcg
     1141 ccgtccgtgc cgggcaggat gccgacgata atggcgatgt tggggcgacc gtcgaggtag
     1201 gtcatcagct tgcgcgcagc ggtgacgata ttgaacagct ggtcagtgcc tcccacctgg
     1261 acatccgctt tcaaggcatg tgcgtcatac ccctgcataa tggagtaaaa cgtttcgtgc
     1321 aggtagatcg gatcgccctt atcccagcgc aggcggaaat tatcgcgggt caggaactgc
     1381 tgcaaggtaa aattggaagc cagtccgatc agctcggcga aggacagctc cgacagccac
     1441 tcggcattga agcgcacttc ggttttggat tcatccagca ctttgaaagc ctgctgagcg
     1501 taggtgcggg cgttcgctac cacctgctca ggggtcagct ggggacgcag cttatcctta
     1561 tcggaagggt cgccgatcaa ggaggtgtag gtgccaacca ggaagatcac ctggtgaccc
     1621 agctcctgga actggcgcag cttgcgcatg ggcacagtat gccccaggtg caggtcgctg
     1681 gtgcggggat caaagccgca gtagacgcgc agggggcgat tttctttctg ggcttcgagc
     1741 aagcgagtac gcagttcgtc tgccatggct tgcttgagcg cttcatcccc gtactcggtg
     1801 ccttgcatca agtaagccac ctgctggtcg attgtcatct ggcggatttc cattcctggc
     1861 ctcccataca aaaataatgg caataaaaag cgccctctgc ggagagggcg cgctgtgctc
     1921 accgcagaac ccggtcaatt gcgggaataa taaaccgtat ctccctggct gtttggagaa
     1981 cgctgcaggt tcgtttcgcg caggatctca gccaggatct tcaccccatc cgggaaaccc
     2041 agccgtccct gcgggtgtcc cagcacttct acttcgctca aataccggaa ttcaaagtgc
     2101 tgcacaccac caacgacctg aacccgcttg cccagcgagc cgctgaagac agtgacctga
     2161 tcgatctggt cccagggcag gctcacccgg cgtaaaccat tggtgaaaaa catgcccctg
     2221 gggtccaggc gcaagaccgt ctggcggtcc atccagttgc tcagcgagat gctggctgcc
     2281 atcaaggcaa ccacgccggc gagatacggc agggagccga ttacggcccc accctgaccg
     2341 cgcagggcac ctgccgccgc acccaggccg agcgcagcca gccaggccag ccc
//
LOCUS       DATMXK010000336         2489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_105572, whole genome shotgun sequence.
ACCESSION   DATMXK010000336 DATMXK010000000
VERSION     DATMXK010000336.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2489
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105572"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1126
                     /locus_tag="VN363_06205"
     CDS             <1..1126
                     /locus_tag="VN363_06205"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84140.1"
                     /translation="VFPTTVHGLIGCTGCHAGQQSPEKEIAHTGLVVNPSDGPTQVCS
                     QCHPHITAAAETSLHNNLKGYTTLLERRGASPHTVEEIMNTSCTACHTSCADCHISQP
                     AVAGGGLIAGHNFTAQPSMERSCTACHGGQVGDEYLGKHSGLEADVHFRQGGLECTAC
                     HTGEKLHGQPDNCTACHPGPESAQLPPASHRYDGVPQPSCESCHASAATKQDDILMHQ
                     LHGSKLACQVCHSVEYANWDGSSIVEDTASGEPVLQPGEMDLAFLIGRNPLQTYSRPY
                     EFVAVRHAPISTTTFDYFEENLLSTFSRVATWQYATPHNIQRVTPQNQSCNYCHGNAE
                     IFLTADKVLPEELQANRSVIVEQIPHLITSADQIPDVFAP"
     gene            1290..>2489
                     /locus_tag="VN363_06210"
     CDS             1290..>2489
                     /locus_tag="VN363_06210"
                     /inference="COORDINATES: protein motif:HMM:NF012437.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009190 - cyclic nucleotide biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0035556 - intracellular signal transduction
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenylate/guanylate cyclase domain-containing
                     protein"
                     /protein_id="HWQ84141.1"
                     /translation="MRCPGCQTENPSIARFCSNCGLSLQNRCPNCRAELAPGARFCMY
                     CGQSLRARSPQDEARLIHLNQAAPAPLVEKARSSNLTGERRMVTVIFADVVGSTVMAL
                     QLGSETWAEVINNAFQVLTPAIYRYEGTIARLVGDSLIAFFGAPVAHEDDPQRAVRAS
                     LEMMAAMRDYSAQVEERYQIRFRMRACLNTGTVLVGPVSDDMRYEFTATAGTVNLAAR
                     IKFAAEPMTVMVTEDTARFIRPYFEILHVDDVSTEERPQRVKIYKVLSIKDQPGKARG
                     LSGLHSSLVGREAPLEALLSQCDLVNAGLGRVSLVVGEPGIGKTRLIEEWKNQVLSGQ
                     RGSGRPPLWVDGRCVSYGGSVPYHLLNDLLHNMTGTSLNSREDEARTALNRLVNELFG
                     NKPGEETA"
BASE COUNT          530 a          751 c          723 g          485 t
ORIGIN      
        1 cgttttcccc accactgtgc acggtctgat cggctgtaca ggctgtcacg ccgggcagca
       61 gtcgccggaa aaagagattg cccataccgg attggtcgta aatcccagtg atggaccaac
      121 ccaggtctgc tcgcagtgcc acccgcatat caccgcagcc gcagaaacca gcctgcataa
      181 caatctgaaa ggttacacca ccctgctgga aaggcgcggc gccagccccc acacggttga
      241 agagatcatg aataccagct gcaccgcctg ccatacttcc tgtgcagact gccatatttc
      301 gcagccggca gtcgccggtg gcggtttgat cgccgggcac aatttcaccg cccagccatc
      361 gatggagcgc agctgcaccg cctgccacgg cgggcaggtc ggcgatgagt acctgggcaa
      421 acacagcggt ctggaagccg atgtccattt ccgccaggga ggattggaat gcactgcctg
      481 ccacaccggc gaaaagctgc acgggcagcc ggataactgc accgcctgcc accccggccc
      541 ggaaagcgcc cagctgccgc ctgccagcca ccgctatgat ggcgtcccgc agcccagctg
      601 cgaatcctgc catgccagtg ccgccaccaa acaggacgat atcctgatgc accagctgca
      661 cggcagcaag ctcgcctgcc aggtgtgcca ctctgtcgaa tatgccaact gggatggctc
      721 cagcattgtg gaagacacag ccagcggcga gcctgtgctc cagcctggcg agatggacct
      781 ggctttcctg atcgggcgaa atccgctcca gacctattcc agaccctacg agtttgtcgc
      841 agtccggcac gccccgatct caaccaccac cttcgattat ttcgaagaaa acctgctgtc
      901 gaccttctct cgggtcgcga cctggcagta tgccaccccg cataatatcc agcgggtaac
      961 tccgcagaac cagtcttgca attactgcca cggcaatgct gagatcttcc tcaccgccga
     1021 taaagttctg ccggaagagc tgcaagccaa tcgcagtgtt atcgtggaac agatcccaca
     1081 tctgatcacg tctgccgatc agatcccgga tgtatttgca ccgtaaacaa accctataag
     1141 ctgtctacgg gctggccttg aaagataagg ccagcccttt ttatcaggta ggctttgtcc
     1201 gaatttagaa tagacaagcc tgcgcttttg tgctagattc taggtggcgt ctcgccaatg
     1261 cgtgagattc gtcaggagga attcttccga tgcgttgccc cggctgccag acggaaaacc
     1321 ccagtattgc tcgattttgt tcgaattgtg ggctgagcct gcaaaatcgc tgcccgaact
     1381 gccgcgctga gctggctccc ggggcgcgtt tctgcatgta ctgcggtcag agcctgcgag
     1441 cacgcagccc ccaggatgag gcgcgcctga tccacttaaa ccaggctgct ccggcaccgc
     1501 tcgttgaaaa agccaggtcg tccaatttaa ccggcgagcg gcggatggtc acagtcattt
     1561 tcgccgacgt ggtcggctcg accgtcatgg cgctgcagct gggcagtgag acctgggcag
     1621 aggtgatcaa caacgctttt caagtcctga ccccggcaat ctatcgctat gaggggacga
     1681 ttgcccggct ggtgggcgat tcgctgatcg catttttcgg agccccggta gcgcacgagg
     1741 acgacccgca gcgtgcagtc cgagcctccc tggagatgat ggcggcgatg cgcgattatt
     1801 ccgcccaggt cgaagaacgc taccagatcc gcttccggat gcgcgcctgc ctgaataccg
     1861 gcacggtcct ggttggcccg gtaagcgatg atatgcgtta cgaattcacg gccactgccg
     1921 ggacggtcaa cctggcggcg cgcatcaaat ttgctgccga gccgatgacg gtgatggtga
     1981 cagaggatac cgcccgtttt atccggcctt atttcgagat cctgcacgtg gatgatgtga
     2041 gcacagaaga gcgcccgcag cgcgtaaaaa tctataaggt gctcagtatc aaagaccagc
     2101 ctggtaaagc gcgcgggttg agcggtttgc acagcagcct ggtcgggcgt gaagcacccc
     2161 tggaagccct gctcagccag tgtgatctgg taaatgccgg tttggggcgg gtgagcctgg
     2221 tggtgggtga gccgggaatt ggcaagacgc gcctgattga agagtggaaa aatcaggtct
     2281 tatccgggca gcgcggcagc gggagacccc cgctgtgggt ggatggacgt tgtgtttcat
     2341 acgggggaag tgtcccctac caccttttga acgatctgct ccataacatg actggcacga
     2401 gcctgaacag ccgggaagat gaagcccgca cggctttgaa ccgcctggta aacgagcttt
     2461 ttgggaataa gccgggcgaa gaaaccgcc
//
LOCUS       DATMXK010000337         8302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_46652, whole genome shotgun sequence.
ACCESSION   DATMXK010000337 DATMXK010000000
VERSION     DATMXK010000337.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8302
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46652"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1235
                     /locus_tag="VN363_06215"
     CDS             <1..1235
                     /locus_tag="VN363_06215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84142.1"
                     /translation="PSPTPPVAVLETDPQPPLQFDLIYLSNNRLMRWDHSRQRQEALI
                     NQVIIYTLSPDGSQIALLRAQGISANGVEMFDLERMNLETREIQILVDDIHEVGALSL
                     SADLKWLAYSIPGDDYTIHLVTNTNEPARQTIASCQPSHPTHCTNLTWSAESDALAWS
                     DREGLWLYTFGGNGSRLILPAEASIPDPRGNTTRVAVRYQSLSWSPFGRYILCEVVVP
                     SSDVRWWGVADTTLENLALLPDSFTTQPPRISAIWAQDGRLFVTRPSHFVENQRAEIE
                     LWNVFPTRSDLFLLDQQFTLPPVEIYTSEQTVSLPDYSLEYPYQVNDYTYHLAAYTTG
                     GTVLSTLLRFDLRFGSLEKTNDLRVVEGNLHWAPDGTSILIGDFEPEWIAAGSPTLYD
                     LQAALGVQSCCFSWIPNP"
     gene            1296..2873
                     /locus_tag="VN363_06220"
     CDS             1296..2873
                     /locus_tag="VN363_06220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84143.1"
                     /translation="MDNLLSLLPAFILALFAAVILVLYLIRPKFRYFWMLAVGGSFLA
                     WLSVWFLRFRLPTSSDILFWQPAGLFPVSPALLLDRISWPYALALSTLALAAILTDIS
                     RSTETHRGNWTGSLAFTALGILAALAGNPLSLMLTWTALDLIELIILIVRVRQNELRP
                     QIFATYAARVAGIFALMAATGVGGELPASFAQISPQNSIFLLLAAGLRLGVFPLHLPF
                     LQQATIERGFSTLLTLVPAGTSLMLLTRVATVITPEPLMTILLLLTGLAVLYASGSWA
                     VATDDLDGRPFWILSFAALAITATLRGEQGASLAFGITGLLSGGLLFLYSTRPRNLLF
                     LPLIGLAWASMLPLTPSWQGVRLYTPPMPLTLLLYLPAQALLLIGYLRHALQPGEPPR
                     RAERWIWLFYPLGLLLIPLTHLIIAWFGNPASRVETDLPRILDSWPGILVLLFAAGIY
                     LAYQRGWRAPAGVIQFIRRSLSLNWFYALFWKIYRWLGRSLEFVSQILEGEGGILWAL
                     LILIVILTLLTQETLGG"
     gene            2874..3554
                     /locus_tag="VN363_06225"
     CDS             2874..3554
                     /locus_tag="VN363_06225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84144.1"
                     /translation="MPELAWLPMVGVLIVIATCFTLLVSSSWQRSLAALAVQYLGQFI
                     LVSSVLPIELALVKLVSGLIAAAVLGMSLSGMLRRGELPSGSTPPDPGDSQIPDPSIA
                     NRRAASPQSLQSASGRIFRLLTGLLAALGGFSVAPLLVEWVPGMSSEQAWGSVILLGV
                     GLLQLGFTVQPFRTILGLLTALSGFEIIYATAESSALVTGLLAFVTLGLSLAGAYLLL
                     VASLEETA"
     gene            3551..4966
                     /locus_tag="VN363_06230"
     CDS             3551..4966
                     /locus_tag="VN363_06230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84145.1"
                     /translation="MNSTFIWVLVPLISGGILLLFNRYEKITAITGTIIAALLAILAW
                     LLPIGETLPFLPSVTIQETLIILGRQFILNNTDRPYLLVIYLIVALWLGGARTAQVGR
                     YMVPVGLIISGLLIAALAVEPPLYAALLIEMSALVSIPVLAPPGKPVSRGVLRFITFQ
                     TLGMPFILSSGWLLPLVVADPTSRPVITAIVLLLLGFALIAAIFPFHTWLVSLTEDIN
                     PYAAAFIFFLMLSNIALFGINFINRLEWLRNFTLLYMILRVMGSLMVLAGGLWAAFQR
                     QLGRMQGQGMMVIIGSSLLALSLGGPDTGFGTPLTTASATGIEFFFAFFLPNALALAV
                     WALALAIIRTQTEILTFRSLQGLAYRLPFASAGLLAAHFSLASLPLLAGFPLYLSLFS
                     RLSAGSFSAAWFAVLGISGLMIGALRTLSVLFSNPEQQTTWSVTETRPQVTLLIIGVA
                     LLLLMGLIPQVFQPVLNIPIP"
     gene            5102..6280
                     /locus_tag="VN363_06235"
     CDS             5102..6280
                     /locus_tag="VN363_06235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84146.1"
                     /translation="MELEQITKQVEWLDDERRKDKTTIGSLEERLSAVEGNLIPLPNQ
                     IKDLGGEITRLTALLARMDHFDEALLQQRIESKQRMEEIERDYKKRLEESERLRKVEM
                     RSLETSLVELRKELEAFPEIRRSLKARVDEESRLAKMIDEVRGRIENMRHSEEEYTRS
                     FRLLDDGRRQDSKRLADVQGELTAVRKRVDEQRGQSELITSAMRKLETRVGEMSTVES
                     ERREAQAKFLETQALRQVERDRVWKDWAARFEEIEAETANIETNIQALDATHRAVKRS
                     QEAVEELSQKVERRITEITEIQRLAEERFRQEWVTFKADDQKRWTNYTLTLEEQRNET
                     ARQFERLIERVTQLEDNSQEVQDLIAQMGEESEKRLQSLLVMAHEWVATYERSQGRSR
                     "
     gene            6313..8235
                     /gene="selB"
                     /locus_tag="VN363_06240"
     CDS             6313..8235
                     /gene="selB"
                     /locus_tag="VN363_06240"
                     /inference="COORDINATES: protein motif:HMM:TIGR00475.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003746 - translation elongation factor
                     activity [Evidence IEA];
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_process: GO:0001514 - selenocysteine incorporation
                     [Evidence IEA];
                     GO_process: GO:0006414 - translational elongation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="selenocysteine-specific translation elongation
                     factor"
                     /protein_id="HWQ84147.1"
                     /translation="MRTIGTAGHVDHGKSTLIEALTGTHPDRLKEEREREMTIDLGFA
                     WMTLPGTEGEPGEEIGIVDVPGHRDFIENMLAGVGAIDAVLFVVAADEGIMPQTREHL
                     AILNLLQIHGGVVALTKIDMVEDPEWLDLVEADLNQAFQGTILADAAIVRVSARTGAG
                     IDNLRAALRDTLAGRPPIADIGRPRLPVDRIFTVAGFGTVVTGTLTDGHIRLGDEVEI
                     LPAGQHGRVRGLQTHKQKESLAVPGSRTAINISGVTVDEIQRGDVVCHPGTYRPTRLI
                     DVHFQLLEDVSQPVRHNTTIKFFTGASESVARLRLLGVEELKPGDSGWLQLELNQPVT
                     VVRGDRYILRRPSPGETLGGGVIVDPAPKGRHKRFAEGVLARLEALFQGTPEDILLQA
                     MLALGAAPVRDVLARSTLQKTTAEQALGNLLADQLILPLGGSETTAVAPETLVISRSY
                     WEQLRERARQEVERYHHSNPLRSGIPREELKSRLKNIPAFTPRLFNLLLTNLAAENEL
                     EEKGPLVKLPGHQIKFTPQQQLRLDSLLKKFAAAPFTPPSAKESAAEIGDDLLAALLD
                     TAELVSVGQDVLFRRIDYEHLVAEIRRLLVLRGTLSAAEVRDQFNTSRKYALALLEHL
                     DAIGVTQRVGDVRRLR"
BASE COUNT         1694 a         2413 c         2235 g         1960 t
ORIGIN      
        1 ccccttctcc tactccgccg gtggcggttt tggaaacgga cccccaacct cctctccaat
       61 ttgacctgat ctatttatcg aataatcgcc tgatgagatg ggatcattct cgccaacgtc
      121 aggaagcttt aatcaaccag gtgatcatct acaccctcag cccggatggc agtcagatcg
      181 ctctgctgcg cgcccaggga atttctgcca atggggttga aatgtttgat ttagagcgca
      241 tgaacctgga aacccgggaa atccagatcc tggtagatga tatccatgag gttggcgcgt
      301 tatctctctc agccgacctg aaatggctgg cgtattcgat ccccggcgat gactatacca
      361 tccacctggt taccaatacc aatgagcctg cccggcaaac cattgcgtcc tgccagccca
      421 gtcaccccac ccactgcact aatttaacct ggtcagcaga aagtgatgcc ttagcctgga
      481 gcgaccgcga aggattatgg ctgtacactt ttggcggtaa cggatcgcga ttaattctcc
      541 ctgctgaagc gtccattccc gatccgcgcg gcaatacaac ccgggtcgca gtccgctatc
      601 agtccttaag ctggtcacca tttgggcgct atattctgtg tgaggttgtc gtaccctctt
      661 cggatgtccg ctggtgggga gtcgccgata caactctgga gaacctggcg ttgctgcccg
      721 attcattcac cactcagcca ccgcgcatct ctgccatctg ggctcaggat ggcaggctct
      781 tcgtaacccg acccagccat tttgtggaaa atcagcgtgc ggagattgag ctgtggaatg
      841 tcttccctac ccgctcagac ctgttcctgc tggaccaaca attcacgctc cccccggtgg
      901 aaatttatac ctccgaacaa accgtaagcc tgcccgatta ttcccttgaa tatccttacc
      961 aggtcaatga ctatacctat cacctggctg catatacgac cgggggtaca gtcctttcta
     1021 ccttgctgcg cttcgatcta cgcttcggca gtctggaaaa aaccaacgac ctgagggttg
     1081 tcgagggaaa cctgcactgg gcacccgatg gaacgagtat tctgatcggc gattttgagc
     1141 ccgagtggat cgctgctggc agcccgacgt tgtacgatct ccaggcagcc ctgggtgttc
     1201 agagctgttg ttttagctgg ataccaaacc cctgatgggc tactcgcatt ggccagctat
     1261 ctggcctgat ctatcgaatc acctgatgcc aatccatgga taacctttta tccctgcttc
     1321 cagcatttat attggccctt tttgccgcgg tcatactggt tttatacctg atccggccaa
     1381 aatttcgcta tttctggatg ctggctgtgg ggggcagctt cctcgcctgg ctgagcgtct
     1441 ggtttttgcg cttccgcctg ccgaccagct cagatatcct gttctggcag cctgccggct
     1501 tgttcccggt atctccagca ctgctgctcg atcgcatttc ctggccttac gcgctggcat
     1561 tatctaccct ggctctggca gccatcctga cggatatcag ccggtctacc gagactcacc
     1621 gcggaaactg gactggcagc ctggcattta cagccttagg gattctggct gctctggctg
     1681 gcaatcctct cagcctgatg ttgacctgga ctgccctgga tctgattgag ctgattatct
     1741 taatcgtccg cgtaaggcaa aatgagctgc gtccgcaaat cttcgccacc tatgcggcgc
     1801 gggtggcagg gatttttgct ctcatggcag caaccggggt aggcggcgag ctgcccgcct
     1861 cttttgccca gatttctcca caaaacagta tctttctcct gcttgctgct ggtctgcgcc
     1921 tgggtgtttt ccctctgcat ttgccattcc tgcagcaggc gacaattgaa cgtggattca
     1981 gcactttact gaccctggtt ccagccggaa ccagcctcat gctgctgaca cgggttgcca
     2041 ctgtaatcac gccagagcca ttgatgacaa ttttactttt gctcaccggt ctggcagtct
     2101 tatatgccag tgggtcctgg gcggtggcaa cagatgattt ggatggaagg cctttctgga
     2161 ttttgtcttt tgctgctctg gcaatcactg ctaccctgcg gggcgaacag ggtgccagcc
     2221 tggcgttcgg gatcacaggt ttgctttcgg gtggattatt gttcctctat tctactcgcc
     2281 cgcgcaattt acttttcctg cctttgatcg gcctggcatg ggcgagcatg ctgccgctca
     2341 cccctagctg gcagggtgtc cgactttaca ctccccccat gccgctcacc ctgctcctgt
     2401 atttgcccgc ccaggcctta ttattaatcg gttacctgcg ccacgcatta caaccgggcg
     2461 agcctccccg ccgggctgaa cgctggatct ggctctttta ccccttgggc ctcctgttga
     2521 taccgcttac ccatctcatc atcgcctggt ttggcaatcc cgcgagcaga gtagaaaccg
     2581 atttgcccag aatcctcgat tcgtggcccg gtatccttgt actgctgttc gcagcgggaa
     2641 tttacctggc ataccagcgt ggctggcgtg cacccgcagg agtgatccaa ttcatacgcc
     2701 gttcgctctc tctaaattgg ttctacgcgc tgttctggaa aatatatcgc tggttgggca
     2761 ggtctcttga atttgttagc cagatcctgg aaggcgaggg aggtatcctg tgggccttgc
     2821 tcatcttaat tgtaatactg actttattaa cccaggaaac cctgggaggg taaatgcctg
     2881 aattagcctg gttgcccatg gtaggtgtct tgattgtcat cgcgacctgc ttcacactgc
     2941 tcgtcagctc gagctggcaa agaagcctgg ctgcgctggc tgtccagtac ctgggccaat
     3001 ttatcctggt cagctcagtc ctgcccatcg agcttgccct ggtaaaactg gtcagcggtt
     3061 tgatagccgc cgctgtcctg gggatgtcgc tatccggtat gcttcggcgg ggagagctgc
     3121 cttctggatc tactccgcct gatcctggtg attcccagat accagatcct tcgatagcca
     3181 atcgccgcgc cgcatcacct cagagcctgc aatcagcctc agggcggata ttccgcctgc
     3241 tcactggatt actggcagcg ctgggaggtt tctcagtcgc tccactgttg gtcgaatggg
     3301 tcccaggaat gagcagcgaa caggcctggg gcagtgtcat ccttttaggg gtaggtctgc
     3361 tccagctggg ttttaccgtc caaccttttc gcaccatcct ggggttgctc acagccctgt
     3421 ctggctttga gatcatttat gccacagcag aatcctctgc cctggttaca ggcctgctgg
     3481 cattcgttac gctgggccta tctcttgccg gcgcgtatct gttgctggta gcctcgctcg
     3541 aggagactgc atgaacagca cgttcatctg ggtcttagtg ccactcatct ccggagggat
     3601 tttattatta ttcaaccgct atgagaaaat cactgcgatt accggaacaa taatcgctgc
     3661 actgctggct attctggcct ggctcctccc aattggcgaa accctgcctt tcttgccttc
     3721 tgtaacgatc caggaaacgc tcattattct cgggcgccaa tttatcttga ataatacaga
     3781 ccggccttat ctgctggtga tttacctgat cgtcgcctta tggctgggag gggcgagaac
     3841 tgcccaggtg ggcagataca tggtgccggt aggcctgatc atcagcggac tgctgatcgc
     3901 agccctggct gttgagccgc cgctctatgc agccctgttg attgaaatgt cggctctggt
     3961 ttccattccc gtcctggctc cgcctggaaa accggtcagc cggggagtgc tccgcttcat
     4021 cactttccaa accctgggta tgccctttat cctttccagc ggctggctgc ttccgcttgt
     4081 ggtcgccgac cctacgagca ggccagttat cactgcgatc gtattactcc tgcttggctt
     4141 tgctctgatt gcggcaatat tccccttcca cacgtggctg gtcagcctga cagaggatat
     4201 caacccatac gcagctgcgt ttattttctt tctaatgctg agcaacattg ccctgtttgg
     4261 gataaatttc atcaaccggc tggaatggct gcgcaatttt accctgctgt atatgatcct
     4321 gcgagtaatg ggcagcctga tggttcttgc tggtggtctt tgggcggctt tccagcgcca
     4381 gctcgggcgc atgcagggcc agggaatgat ggtaatcatc ggcagctcct tgctggcgtt
     4441 gagcctcggc ggacctgaca ccggttttgg aaccccgctg actaccgcca gcgcgaccgg
     4501 gatcgaattt ttcttcgcct ttttcctgcc aaatgcgctg gccctggcag tttgggcgct
     4561 ggctctggca atcatccgca cccaaaccga aattctgact tttcgcagtc ttcaaggact
     4621 ggcttaccgg ctcccattcg ccagcgccgg actgctggcc gcccatttct cgctcgccag
     4681 tctcccgctt ctggccggct ttccgctcta tctctccctg ttttccaggc tcagtgccgg
     4741 atcattttcc gccgcctggt ttgctgtcct cggtatcagt ggattaatga ttggggcttt
     4801 acgcaccctg tcagtgttat tcagcaatcc agagcagcaa accacctggt cggtcacaga
     4861 aactcgccca caagttacac tgctaatcat tggtgtggcc ctgctcttgc tcatgggttt
     4921 aatcccgcag gtttttcagc cagtgttgaa tatccccatc ccataataag ccaggcaccc
     4981 aaccgcaccg tcaacgatgc cagcgcctgg caatcgcctg gacaggcatg ctataatctt
     5041 caaatacctg aatgtcctct gccagactga ggactgaact tgagaccaag gagaccggcc
     5101 gatggaattg gaacaaatta caaaacaagt agaatggctg gacgacgaac gtagaaaaga
     5161 taagactacc atcggatccc tggaagaacg cctgtcagca gtggaaggca acttgatccc
     5221 attgccgaac cagatcaaag atctcggcgg tgagattact cgcctcacag ccctgctggc
     5281 gcgcatggat catttcgatg aagctttgct ccagcagcgg attgaatcca aacagcggat
     5341 ggaagagatc gaacgcgatt ataaaaaacg cctggaagaa agcgaacgct tgcgtaaggt
     5401 agagatgcgt tcactggaaa ccagcctggt ggaattacgt aaagaattgg aagctttccc
     5461 cgagataaga cgcagcctga aagcccgggt agatgaggaa tcccggttgg caaagatgat
     5521 cgacgaggta cgcgggcgga tcgaaaacat gcgccacagt gaagaagaat atacccgctc
     5581 ctttcgcctg ctggatgatg gccggcggca ggactctaaa cgtctggctg atgtccaggg
     5641 tgaattaacc gccgttcgca aacgtgtgga cgagcagcgc gggcagagtg aattaattac
     5701 ttcagccatg cgcaagctcg agacccgcgt gggagagatg tctaccgtgg aatccgagcg
     5761 ccgggaagcc caggcaaagt tcctcgagac gcaggctctc cgccaggttg agcgcgaccg
     5821 cgtctggaaa gattgggcag cccgctttga agagatcgag gccgaaaccg ccaacatcga
     5881 aaccaatatc caggccctgg atgcgaccca ccgggcagtc aaacgctcac aagaagccgt
     5941 ggaagaactg agccagaaag ttgagcggcg aatcaccgag attaccgaga tccagcgcct
     6001 cgcagaagag cgctttcgcc aggaatgggt aaccttcaag gcggatgatc aaaaacgctg
     6061 gaccaactac accctgaccc tggaggagca gcgcaatgag actgcccgcc agtttgaacg
     6121 cttgatcgag cgcgttaccc agctggagga taattctcag gaagtccagg atttgattgc
     6181 ccagatgggc gaagaatccg agaaacgcct ccagtctctg ctggtgatgg cccacgaatg
     6241 ggttgcaacc tacgagcgca gccagggacg cagccgctga cctctttaac ctgagtaaga
     6301 tcgaggagtt gcatgcgtac catcggcact gccggccatg tagaccatgg taaatccacc
     6361 ctgatcgagg ctctcaccgg aacacacccg gatcgcctga aggaagagcg cgagcgggag
     6421 atgacgatcg atctcggctt tgcctggatg accctgcccg gaaccgaagg cgagccaggt
     6481 gaggaaattg gcattgtcga tgtcccgggt catcgtgatt tcatcgagaa tatgctggcg
     6541 ggtgtcgggg cgatcgatgc agtcctgttc gtggtggcag ccgatgaagg gatcatgccc
     6601 cagacccgtg agcatctggc aatcctcaac ctcctgcaaa tccatggcgg tgtggtcgcc
     6661 ctgaccaaga tcgatatggt ggaagacccc gaatggctgg atctggtcga agccgatctg
     6721 aaccaggcat tccagggaac gatcctggcc gatgctgcaa ttgtgcgcgt ttccgcccgc
     6781 accggcgcgg gaatcgataa cctgcgggcg gccttacgcg atacgcttgc cggtcgccct
     6841 ccaatagcgg atattggccg gccgcgcttg cccgtcgatc gcattttcac cgtggcgggg
     6901 tttggcacag tggttaccgg cacattaacc gatggtcata tccgtctggg ggatgaggtt
     6961 gaaattctgc ctgccgggca gcatgggcgc gtgcgcggtt tgcaaaccca taaacaaaaa
     7021 gaaagccttg cagtccccgg cagccggacg gcgatcaata tttccggggt tactgttgac
     7081 gagatccagc gtggggatgt ggtgtgccat ccaggaacgt accgccctac ccgcctgatc
     7141 gatgtccact tccagctgct cgaagatgtc agccagccag tcaggcacaa cactacgatc
     7201 aagtttttca ccggggcctc cgaatcggtc gcccgcctgc gtcttctggg cgtcgaggag
     7261 ctgaaacccg gtgattctgg ctggctgcag ctggagctga accaacctgt gacagtcgta
     7321 cgcggcgacc gctatatcct gcgccggcct tccccgggcg agaccctggg cggtggcgtg
     7381 attgtcgacc cggcacctaa aggccggcac aaacgctttg cggagggcgt gctggcgcgc
     7441 ctggaagccc tcttccaggg cacgccggag gatatcctcc tgcaagccat gctggctctc
     7501 ggcgcagctc cggtgcgaga tgtgctggcg cggtctaccc tccagaagac cactgccgag
     7561 caagctctgg gtaatctcct tgccgatcaa ttgatcctgc ccctgggtgg ttctgagact
     7621 accgcggttg cccccgagac cctggtgatc agccgcagtt attgggaaca gctgcgcgag
     7681 cgcgccaggc aagaagttga gcgctaccat cacagcaatc cactgcgttc aggcatcccc
     7741 cgcgaagaat taaaaagtcg cctgaaaaac attcccgcat ttactccccg cctgttcaac
     7801 ctcctgttga ccaatctggc agctgaaaat gagctggaag aaaaagggcc cctggtaaag
     7861 ctgcccggcc accagatcaa atttacccca cagcagcagc tgcgcctgga cagcttgctc
     7921 aagaaatttg ctgctgctcc tttcactccc ccttctgcca aagaaagtgc ggctgagatc
     7981 ggcgacgacc tgctggctgc tctgctggat actgcagagc tggtctcagt gggacaggat
     8041 gtgctcttcc ggcgaatcga ttacgaacac ctggttgcag agattcgccg gctgctggtc
     8101 ctgcgcggca cattgagcgc tgctgaagtg cgcgatcagt tcaacaccag ccgtaaatac
     8161 gccctggcat tgctggagca tctggacgca attggagtca cccagcgggt aggagatgtg
     8221 cgtcgcctgc gctgattcgt cgccggcacg aattcgttat ttgcgtaaac gcgaacttat
     8281 gctaatctat accatatagc ag
//
LOCUS       DATMXK010000338         2013 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161025, whole genome shotgun sequence.
ACCESSION   DATMXK010000338 DATMXK010000000
VERSION     DATMXK010000338.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2013)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2013)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2013
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161025"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..747)
                     /locus_tag="VN363_06245"
     CDS             complement(<1..747)
                     /locus_tag="VN363_06245"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560499.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWQ84148.1"
                     /translation="MNFERSSGIILHPTSLPGPYGIGDLGPQAHAWIDFLADNDSNLW
                     QVLPIGPTGYGDSPYQSFSAFAGNPYLISPDALLAEDLLTTDDLGTLPHFPARRTDYG
                     AVIPWKLALLDRSYAYFLSRANPDQMESYRQFQAEQAAWLVDFAVFMALKDAHGGAPW
                     PTWEPALRDRDPQALAAFRQANSLQIERQTYRQFLFFRQWSALKAHAVQRKIKIIGDI
                     PIFAAHDSADVWAHPELFYLDEKGKPTSVAG"
     gene            complement(737..1996)
                     /gene="glgC"
                     /locus_tag="VN363_06250"
     CDS             complement(737..1996)
                     /gene="glgC"
                     /locus_tag="VN363_06250"
                     /EC_number="2.7.7.27"
                     /inference="COORDINATES: protein motif:HMM:TIGR02091.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008878 - glucose-1-phosphate
                     adenylyltransferase activity [Evidence IEA];
                     GO_process: GO:0005978 - glycogen biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glucose-1-phosphate adenylyltransferase"
                     /protein_id="HWQ84149.1"
                     /translation="MKTRAIILAGGEGSRLGVMTAKRTKPAVPFAGKYRIIDFTLSNC
                     VNSDIYDVMIMAQYRPQSLIEHIGAGGPWDLNREFSGGVRILTPYKARGASDWYLGTA
                     DAVQQNFLFIKQGRPDNILILSGDHIYEMNYDALVTFHRDQNADLTMATISVPLAEAS
                     RFGIVGVGDDYRVTSFVEKPKEPPSTLANMGVYLFNLDTLDAALWDDHNDPHSDHDFG
                     KNILPRLINQGKRVFAFPYSGYWVDVGTVASYWQAHMDLLKDPPSIDMNDRSWIIHTR
                     TEERPPARIASGATIKDSMISDGCVIASGAEIERSVLAPGVVVKSGAVVRESIILTEV
                     VIESGAVIQRTVIDKRAKIGANARVGGMLSDGLRISMVGKHSILPEGAVVEPGGMIAT
                     DVIPSDLPSLTIRGDDYIQTKRLPNEL"
BASE COUNT          375 a          593 c          652 g          393 t
ORIGIN      
        1 cccggcgacg gaggtgggct tgcccttttc gtcaaggtag aacagctcgg ggtgcgccca
       61 gacatcggcg ctgtcgtggg cggcgaaaat cgggatatcg cctatgatct tgatcttgcg
      121 ctggacggca tgcgccttca gcgccgacca ctggcggaag aacaggaact ggcgatacgt
      181 ctggcgctcg atctgcaggc tgtttgcctg gcgaaaagct gccagcgcct gggggtcgcg
      241 gtcgcgcagc gcaggctccc aggtgggcca gggggccccg ccgtgggcat ccttgagcgc
      301 catgaatacg gcgaaatcca ccagccaggc ggcctgctct gcctggaact gccggtagga
      361 ttccatctga tcagggttgg cgcgactgag aaaataagcg taagagcggt ccagcagggc
      421 gagcttccag ggaatgaccg ccccgtaatc ggtgcgccgg gcggggaaat gcggcagggt
      481 gcccagatcg tcggttgtca gcaggtcttc ggccagcagg gcatccgggc tgatcaagta
      541 gggattcccg gcgaaggccg aaaaggactg gtacggcgag tccccataac cggtgggtcc
      601 aatcggcagc acctgccaca ggttgctgtc gttgtctgcc aggaaatcga tccaggcatg
      661 cgcctgcggc cccaggtcgc cgatgccgta cggcccgggc aggctggtgg gatgtaagat
      721 gatccccgat gatcgctcaa agttcattcg gtagcctctt ggtttgaatg taatcgtctc
      781 cccggatggt gagggagggc agatcggagg ggatcacatc ggtggcgatc atcccgccgg
      841 gctccaccac cgcaccttcc ggcaggatcg aatgtttacc gaccatgctg atccgcagcc
      901 cgtcgctgag catgccgccg acccgggcat tggcgccgat cttcgcccgt ttgtcgatga
      961 cggtgcgctg gatgacagcc ccactttcga ttaccacttc ggtgaggatg atcgactcgc
     1021 gcaccactgc cccgctcttg accaccacgc cgggcgccag cacgctgcgt tcaatttcag
     1081 ccccggaggc gattacgcag ccatcactga tcatgctgtc cttgatggtt gccccgctgg
     1141 cgatgcgcgc cggcgggcgt tcttcggtgc gggtgtggat gatccacgag cggtcgttca
     1201 tatcgatcga cgggggatcc ttgagcaggt ccatatgcgc ctgccagtaa gaagccaccg
     1261 tgcccacatc cacccagtag ccggagtagg ggaaagcgaa tacgcgcttg ccctggttga
     1321 tcaggcgcgg caggatattc ttgccaaaat cgtgatcgga atgaggatcg ttgtggtcgt
     1381 cccacaaggc ggcgtccagg gtgtccagat tgaacaggta cacacccata ttcgccaggg
     1441 tggacggagg ttctttgggc ttttcgacga acgaagtcac ccggtaatcg tcgcccacgc
     1501 ccacgatccc aaagcggctg gcttcggcaa gcgggacgct gatcgtcgcc atggtcaaat
     1561 ctgcattctg gtcgcggtgg aaggtgacca gggcatcata attcatctcg tagatgtggt
     1621 cgccggacag gatcaggatg ttgtcgggcc ggccctgctt gatgaacagg aaattctgct
     1681 ggacggcatc ggccgtgccc aagtaccagt cggaggcgcc gcgggctttg taaggggtca
     1741 ggatgcgcac cccccctgag aactcccggt tcaggtccca gggaccgccg gcgccaatgt
     1801 gctcgatcag tgactgcggc cggtactggg ccatgatcat cacgtcgtag atgtccgaat
     1861 ttacgcagtt ggacagggtg aaatcgataa tccggtattt gccggcaaag ggtacggccg
     1921 gcttggtgcg cttggcggtc atcacgccca gccgggagcc ttcgccgcca gccagaataa
     1981 ttgctctggt tttcatgccc accctcctta tgc
//
LOCUS       DATMXK010000339         4198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_106940, whole genome shotgun sequence.
ACCESSION   DATMXK010000339 DATMXK010000000
VERSION     DATMXK010000339.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4198
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_106940"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..445
                     /locus_tag="VN363_06255"
     CDS             <1..445
                     /locus_tag="VN363_06255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84150.1"
                     /translation="SYTLETLIQAGSRHIAVEYVPVRTNPQIRPSRLMRSIPHYLAHS
                     GATILRAYTMYRPLRVFSGFSLLFLVVGLFLSLRFVYYYVQGQGDGHVQSLILAAVLL
                     IVGFQTFLIGLVADLINSNRKILEDLIYRIRRLELDKHPQDQPPQ"
     gene            445..2877
                     /locus_tag="VN363_06260"
     CDS             445..2877
                     /locus_tag="VN363_06260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84151.1"
                     /translation="MKNRIIGLILISIYLLLLNACAPIVRSDQPAHTGWIILDSGHSL
                     GQTFTARYAGMEGIAFLIEPQQSAEGSLELNLYAYNQREIPLSSAAIVINPDSPTGFQ
                     RFTLGKISLSNQQDYYVRLVWQGTGKVTVGAGPGESYLNGAAYLDEVPQDQQTVFRLV
                     YSPTTAARGLLLEGISWLLNLSLGLLLFVIPGWGILTILFPGWHDFFWAEKIGLAAGV
                     SLAIYPILMVWTDLIGIHLGVFNAWIPIVPGISLMFWRFANRPHNIKSIPKPNIPNLL
                     CNLAYAGVIAIILLTRLWPMRGLDAPMWGDGLQHTIITRLLVDHNGLFTNWAPYAELT
                     SFTYHFGFHSLAAVFHWMTGMEASQAVLWTGQFVNVLSLLVLVPLANRLARNRWAGVI
                     ALAVAGLLTSIPQVYLNWGRYTQLAGQVILLVFVVLAWEYLENHSQKSSMAILVIGLL
                     AGLALTHNRVSVFGAAFLVIYFLFNVSKAAFWRTTRSYLVIASGTLLLLLPWLVRTIP
                     GAVTQLLVSLLTKPVNQLSETTTQAESILQFVGYIPQPIWYLSGIAIILGIFRRDKAL
                     LVSVFWWLLILLAGYPQWLGLPGYGALGGFTVMIAAYIPAAVLVSYLASQLLDILDKL
                     ERPRFNKPLLFGLVFAALFGLSIYNIRPRLQEIDPRQSALFTRADQRASEWINAHLPE
                     DSLILINSFLAYDNTLAVGSDGGWWLPVKAHRPVTVPPINYVFEDHQDPTFQQRILEL
                     STAVQDPGLADSEVQNMLREWGITHIYIGQQQGRVNNFGPTMTPEQLLQLDTFTPIYH
                     EDRVWVFERSEP"
     gene            2874..4025
                     /locus_tag="VN363_06265"
     CDS             2874..4025
                     /locus_tag="VN363_06265"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ84152.1"
                     /translation="MNEKRSTPLRVCYFGTYRAEYERNQMMIQRLERAGIEVVVCHAT
                     LWGDFEDREQSASKGWKQPAFWLRVIKAYWQLLRRYRQVGRYDVLMLGYPGQPDVLLG
                     RILANLRRKPLVWDVFMSIYLIAQERGLEDRSPFILKFLYWLEKRAIKLPDLVILDIP
                     TYAAWYRKEYGIADDKIRLIPIGTDDRIFHPDPERINESRQEFICLYYGTYIPNHGVS
                     FIVEAARILEAYPNIQFKLVGKGPDRAKAEQAAIHYGLNNVTFIDWQEKPALIETIQD
                     SDVILGTFGSTPQALMTMQHKIHEGLAMAKPVINGDSPAMRALLEHGSEIFLCRREDP
                     QALAEAILFLAEHPEACEKLSKNGYAYYQTNLRDDLLSQRLGEIVRSIA"
BASE COUNT          953 a         1115 c         1071 g         1059 t
ORIGIN      
        1 ttcctataca ctggagacat taattcaggc tggctcgcgg catatcgctg tggaatatgt
       61 gccggtgcgc accaatcccc agatccgacc ttcacgcctg atgcgcagca tcccccatta
      121 tctcgcccac tccggggcta caatccttcg agcatatacc atgtacagac ctctccgcgt
      181 cttttctggc ttcagcctgc tctttctggt tgttggctta ttcctcagct tgcgcttcgt
      241 ttattattac gtccagggtc agggtgatgg acatgttcaa tctttaattc tggcggcagt
      301 cttattgatc gttgggtttc aaacgttttt gatcggactg gttgctgacc tgatcaactc
      361 aaaccggaaa atcctggaag atttgatcta tcgtatccgg cgtttggagc tcgataagca
      421 tccccaggac caaccccctc agtgatgaaa aaccggatta ttggcctgat cctgatcagc
      481 atttacctgc tgctcttgaa tgcttgtgcg ccaatcgtaa gatccgacca accagcgcat
      541 acgggctgga ttattctgga ttccggacac agtctgggtc aaacattcac agcccggtac
      601 gccggaatgg aagggattgc gtttctgatc gagccccagc agtctgcaga gggcagcctg
      661 gagctgaatc tatacgccta caaccagcgc gaaatacccc ttagcagcgc agcaatcgtt
      721 atcaatccag atagtccgac cggttttcag cgattcaccc tcgggaaaat ttccctatcc
      781 aaccagcaag attattatgt caggctcgtc tggcagggta cagggaaagt cactgtgggt
      841 gcaggtccag gggagagtta tttaaatgga gcagcctacc tggatgaagt tcctcaggat
      901 cagcaaactg tctttcgtct ggtgtattca cccaccacag ctgcccgcgg tctgctcctg
      961 gaaggaatta gctggctctt gaatctcagc ctcggattac tattattcgt tattccgggc
     1021 tggggcattt tgacgatcct tttcccgggc tggcacgatt tcttctgggc cgagaaaatt
     1081 ggtctggccg ctggagtaag cctggcaatc taccccatcc tgatggtgtg gaccgacttg
     1141 atcggcatcc atctgggggt gtttaacgcc tggatcccaa ttgtgccggg gataagcctg
     1201 atgttctgga ggtttgccaa tcgcccgcac aatattaaat ccatcccgaa accaaatata
     1261 cccaatctac tctgtaacct ggcttatgcc ggggttatcg ccatcatctt gcttacgcgc
     1321 ttgtggccta tgcgcggcct ggatgcaccc atgtggggcg atgggctgca gcacaccatc
     1381 atcacccggc tgcttgtgga tcataatgga ttattcacaa actgggcgcc gtacgctgaa
     1441 ttaaccagct tcacgtatca cttcggcttt cattcgctgg cagccgtctt tcactggatg
     1501 accgggatgg aagcgtctca ggctgtcttg tggacgggcc agttcgtgaa tgtcttgagc
     1561 ttgctggtcc tggtccctct ggcaaatcgc ctggcacgaa atcgatgggc gggagtcatt
     1621 gctcttgctg tagcgggttt gctcacctcg atcccgcagg tttatttgaa ctggggacgt
     1681 tacacgcagc tcgccgggca ggtcatcctg ctggtatttg tcgtgctagc ctgggaatat
     1741 ctggagaatc attctcaaaa aagttccatg gcgatcctgg tgattggact gctggcaggc
     1801 ctcgccctga cccataatcg tgtatccgtc tttggcgcag ccttcctggt gatctatttc
     1861 ttattcaatg taagtaaggc agcattctgg cggacaacca gatcctatct ggtgattgcc
     1921 tcaggtacgt tactgctcct gctgccctgg ttggtacgga ccatccctgg cgcagtgacc
     1981 cagctgctgg tctctctgct cacaaagccc gtcaaccagc tttcagagac caccacacag
     2041 gcagaaagta ttctgcaatt tgtagggtat atcccacagc caatctggta cctgagtggg
     2101 atcgcgataa ttttgggaat attccggcgg gacaaagccc tgctcgtaag tgtattctgg
     2161 tggctgctta tcctgctggc aggatacccg cagtggctcg gattgccggg ttacggcgcg
     2221 ctcggcggct tcacggtgat gatcgcagcc tacattccgg cagcagtcct ggtaagctat
     2281 cttgccagcc agctattgga tatcctggat aaactagagc gaccccgttt caataagcca
     2341 cttctatttg ggcttgtgtt tgcagccctg tttggcttat cgatctataa tatccggccg
     2401 cgcctgcaag agattgatcc acgccaaagt gcgctattta cccgcgccga ccagcgcgcc
     2461 tccgagtgga taaatgccca tctaccagaa gacagcctga tcttgatcaa ctccttcctg
     2521 gcttatgaca ataccctggc agtcggatcc gatggcggct ggtggctgcc ggtcaaagct
     2581 caccgaccag taaccgtgcc cccgattaat tatgtatttg aagatcatca agatccaact
     2641 ttccaacaaa ggattctcga gctatccaca gcagtccagg atcctggtct tgcagattct
     2701 gaagtccaaa atatgttacg tgaatggggt atcacccata tctatatcgg gcagcagcag
     2761 ggtcgcgtga acaattttgg gccgacgatg acccccgaac agctgctgca gctggataca
     2821 tttacaccga tctatcatga agatcgggtc tgggttttcg aacgctccga gccatgaacg
     2881 agaaacgaag tactccattg cgagtctgct atttcggcac ctaccgcgcc gaatatgagc
     2941 gcaaccagat gatgatccag cgcctcgagc gggccgggat cgaggtcgtc gtttgccatg
     3001 ccaccttatg gggtgacttt gaagatcgtg agcaatctgc cagcaaaggc tggaaacaac
     3061 cggctttctg gctgcgggtg atcaaggctt actggcagct gctgcgcaga taccggcagg
     3121 ttgggagata tgatgtgctg atgctgggtt acccgggcca gccagatgtt ctgctgggac
     3181 gcattcttgc gaatttacgc cgcaaacccc tggtctggga cgtgtttatg tcgatctacc
     3241 tgatcgcaca ggaacgcggc ctggaggatc gcagtccatt catcctgaaa tttctgtact
     3301 ggctggaaaa gcgcgccata aaactgcctg atctggttat cttggatatt ccaacttatg
     3361 ccgcatggta tcgaaaagag tacgggatcg cggatgataa gatccggctg atcccgattg
     3421 ggacagatga tcgcattttc catccagatc ctgaacgcat caatgagtcc aggcaagaat
     3481 ttatctgcct gtattatggg acctacattc ccaaccacgg agtatccttc attgtcgagg
     3541 ccgctcgcat actcgaggct tacccaaata ttcaattcaa gctggttggg aaaggtccag
     3601 accgtgctaa ggctgaacaa gctgccatcc actatgggct taataatgta actttcatcg
     3661 attggcagga aaaaccagcc ctgatcgaaa ctatccagga cagtgatgtg atcctgggga
     3721 ctttcgggag cacacctcag gcattgatga ccatgcagca caagatccat gaaggcctgg
     3781 cgatggcaaa gccggtgatc aatggggatt cgccggcaat gagagcctta ttggaacacg
     3841 ggagtgaaat cttcctctgc cggcgggaag acccgcaggc cctggctgaa gcaattctat
     3901 ttctggccga acatcctgaa gcgtgcgaaa aactttccaa aaatggatac gcctattatc
     3961 agacgaatct gagagatgat ttgctctccc aaagactggg cgaaattgtg cgctcgattg
     4021 cctgagtgac cccatttgag tggtccaggt gctcagcagg ccgcctgata taatggtaac
     4081 caaatactct caggaaatag aacaatcgtg gaattttccg tcgttattcc aaatctaaat
     4141 tcaccgatca tcaaccagac gattgatgcg atctccagcc aaaattttaa cccgcagc
//
LOCUS       DATMXK010000340         3160 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_24268, whole genome shotgun sequence.
ACCESSION   DATMXK010000340 DATMXK010000000
VERSION     DATMXK010000340.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3160)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3160)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3160
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24268"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2602
                     /locus_tag="VN363_06270"
     CDS             <1..2602
                     /locus_tag="VN363_06270"
                     /inference="COORDINATES: protein motif:HMM:NF020246.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="WD40 repeat domain-containing protein"
                     /protein_id="HWQ84153.1"
                     /translation="LYSGARLATAREGLDSGQLSLGGLPLEFVQAAIQRQEAERLARR
                     QRSQRTIIGLAAGVVVFAVLAGISLVFWRQAQASAHVAEEQAGAAAALLGLSQERGTE
                     AANERTQALAARATADFNASVAVAQEEEARRQATLALSRQLASQALSNLLTGEIDTAL
                     LLAVQAYQAADTPQARSALLSTIEENPRLVNLLTGQHDFVLDMAYSPDGRMLASAGCA
                     QRDLGRENCLRGELRVWDASSGRLLAELGKQFPEIHSNAITRIAFSPNGRYLASGDRS
                     GNVALWDAIRLQPLTAVMGEMEEEIGYLAFNSRSTVLIAGDGQTTQSWNVINQQAQGN
                     PYIEIPELVDEQAPAAIAGDGSLLAIRTPDYLVIWDTRIVNESTRLYTGSFKDPPGSL
                     TFDPDGNWLAFTQENEAYAISTTNAEIITQPLQGHTQRITRLAFNPIFSNVLASGGWD
                     TSIRLWDLANWLAADKPMEQQTTPSGAAQYAPLTGHHDYITSLIFSPDGNTLASGSRD
                     TTIRLWNWQGTPSLADNYYQGTEELFGLTYTPDGRHIAIAVCSQAAENGEDCLSSEVL
                     LLDASSGEMVGEPMTGAANLLTNIAIDPAGKLLAAAGWEGKIYLWDLTSGESVGESLS
                     GHTDFVTGLGFSPDGKLLASAGRDGQVLVWNVAERQLAMDRLTGHTERITSLDFSPDG
                     TRLVTGSRDQNVIIWQINTAPVTSTTLTGHQEQVNLVRFSPDGSLAVSGSADGQMILW
                     DVASGSQLQTIQAHKERLSALAFSPDGRSLATGSWDRTVQLWDVETGQPVGRPLSGHG
                     DTINGLAFSPDGETLSSSSRDGTILRWNVDPAAWVQRACQIAGRALSENEWRSFAGPN
                     VPYSPVCAAP"
     gene            complement(2624..>3160)
                     /locus_tag="VN363_06275"
     CDS             complement(2624..>3160)
                     /locus_tag="VN363_06275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF013827.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="rhomboid family intramembrane serine protease"
                     /protein_id="HWQ84154.1"
                     /translation="LFTPMLLHGSLLHIGFNMYALYALGPGLESYYGHSRFLVLYILS
                     GFTGNVLSFLFSPANSLGSSTAIFGLLGAQAVFLFQNKSLFGSRAQRALMNVAMVAAV
                     NLFIGFTSSGIDNWGHLGGMAGGLIFSWFAGPRLTVQGLAPSYILVDERQKTHVVWSA
                     VLVGGLFAALALAGILTR"
BASE COUNT          736 a          868 c         1010 g          546 t
ORIGIN      
        1 tctctattca ggtgcgcggc tggcgactgc cagggaaggg ttggacagcg gacagctaag
       61 cctgggtggg ctgccgcttg aatttgtgca ggcggcgatc cagcgccagg aagccgaacg
      121 ccttgcgcgg cggcagcgca gccagcgcac catcatcggg ctggcggcag gggtggtggt
      181 ctttgcagtg ctggcgggaa tcagcctggt tttttggagg caggcccagg ccagcgctca
      241 cgtcgcggaa gaacaggcag gggcggccgc agccctgctg ggcttatccc aggaacgcgg
      301 cacggaggcc gcgaatgaac gcacccaggc actggcggca cgggccacgg cagattttaa
      361 tgccagcgtg gctgtggcgc aggaagaaga agcccgccgc caggcaaccc tggccctttc
      421 ccggcagtta gcctctcagg cgctatccaa tttgctcaca ggcgaaatcg ataccgcgct
      481 gctgctggca gtccaggctt accaggctgc cgatacgcct caggcgcgca gcgcgctgct
      541 gtccaccatc gaggagaacc cgcgcctggt caacttactc accgggcagc atgattttgt
      601 gctcgacatg gcgtacagcc cggatggacg aatgctggcc tcggcaggct gcgcgcagcg
      661 cgatctgggg cgggaaaact gcctgcgcgg cgagctgcgg gtgtgggatg cgtccagcgg
      721 gcggctgctg gctgagctgg gcaagcagtt tcctgaaatc cactccaatg caattaccag
      781 gatcgccttc agcccaaatg ggcgctacct ggcgagcggt gaccggagtg ggaatgtcgc
      841 cttgtgggac gcgatcagac tgcagccgct gacagccgtg atgggggaga tggaggaaga
      901 gatcggctat ctggcattta actcgcgcag cacggtgctg attgccggcg acgggcaaac
      961 gacccaaagc tggaatgtga tcaaccagca ggcgcaagga aatccgtata ttgagatacc
     1021 cgagctggtt gatgaacagg cgcctgcagc catcgcaggt gatgggtcgc tgctggcgat
     1081 caggacgcca gattatctgg ttatttggga cacgcggatt gtcaatgaat ctacacgcct
     1141 ttatactggt tcgttcaagg acccaccggg atctttaacg ttcgatccag atggaaactg
     1201 gctggcgttt acgcaggaaa atgaggcgta cgccatatcc accacaaatg ccgagatcat
     1261 cacccaaccc ttacaggggc atacacagcg aataacccgc ctggccttca acccaatatt
     1321 ctcgaatgtg ctggccagcg gcggttggga tacaagcatc cgcctgtggg atctggcgaa
     1381 ctggctggca gcggacaaac ccatggaaca acaaaccacc cccagcggtg cggcgcagta
     1441 tgcgccgctg accggtcacc acgactatat caccagcctg atcttcagcc cggacggtaa
     1501 cactctggct tctggcagcc gggatacgac catccggttg tggaactggc agggcacacc
     1561 cagcctggcg gacaattatt accagggaac ggaagaatta ttcgggttaa cttatacacc
     1621 ggatggacgt cacatcgcca tcgctgtatg cagccaggca gcggaaaatg gggaggactg
     1681 cctgagcagc gaggtgctgc tcctggatgc cagcagcggg gaaatggtgg gagaaccgat
     1741 gacaggggcc gccaacctgc tgacaaatat tgctatcgat ccggcaggga agctgctggc
     1801 tgctgcagga tgggaaggga aaatctacct gtgggacctg accagcgggg aatcggttgg
     1861 tgagtcattg agcgggcata ccgatttcgt cacgggattg ggcttcagcc cggatggcaa
     1921 gctgctggct tctgccgggc gagatggaca ggtactggta tggaatgtcg ccgagcggca
     1981 gctggcaatg gatcgattaa ccgggcatac ggagaggatc accagtctgg atttcagccc
     2041 ggatgggacg cgcctggtga ccggaagccg cgaccagaac gtgatcatct ggcaaataaa
     2101 cacggcacca gtgaccagca ctaccttaac cgggcatcaa gagcaagtca acctggtgcg
     2161 cttcagcccg gatggctcgt tagcggtctc gggcagcgcg gatggacaga tgattctatg
     2221 ggatgttgcg agcggcagcc agctgcaaac aatccaggcg cataaagaac ggctgagcgc
     2281 cctggctttc agtccggacg ggcgcagcct ggctaccgga agctgggaca ggacggtcca
     2341 actctgggat gtagaaaccg ggcagccggt tgggcgccca ttgagcgggc atggagacac
     2401 cattaacggg ttggcattca gcccggatgg ggaaacttta tcctccagca gccgggatgg
     2461 aacgatctta cgctggaatg tagatccggc ggcatgggtg cagcgagcct gccagatcgc
     2521 agggcgcgct ttgagcgaaa atgaatggcg gagttttgcc gggccgaacg tcccttacag
     2581 cccggtttgc gctgcaccat gagatcctag cgtaaaggcc tgcctatctg gtcagaatgc
     2641 cggccagagc cagggcagca aataatccac ccaccaacac tgcggaccaa accacatggg
     2701 ttttctggcg ctcatcgacc aggatgtagc tgggcgcgag accctgaaca gtcaagcgcg
     2761 ggccggcaaa ccagctaaaa atcaacccac ctgccatacc gcccagatgg ccccagttat
     2821 caatcccgga ggatgtaaaa ccaatgaaca ggttgaccgc ggctaccatg gcgacattca
     2881 tcaaagctcg ctgggcacgc gagccgaaga ggcttttatt ctgaaagagg aaaaccgctt
     2941 gagcaccgag gaggccgaag atggcggtcg aagagcctaa cgaattggcg ggagaaaata
     3001 gaaaagacag cacattgccc gtaaatccag aaagtatgta aagcaccagg aagcgcgaat
     3061 gaccataata gctttccagg ccaggcccga gggcatagag ggcatacata ttaaagccga
     3121 tatgcaagag cgagccgtgc aagagcatgg gggtgaagag
//
LOCUS       DATMXK010000341         3513 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_16881, whole genome shotgun sequence.
ACCESSION   DATMXK010000341 DATMXK010000000
VERSION     DATMXK010000341.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3513)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3513)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3513
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16881"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2071
                     /locus_tag="VN363_06280"
     CDS             <1..2071
                     /locus_tag="VN363_06280"
                     /inference="COORDINATES: protein motif:HMM:NF013524.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008234 - cysteine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="C25 family cysteine peptidase"
                     /protein_id="HWQ84155.1"
                     /translation="PHMAAAMFEKYTDQNVYWLDIDGPAGLRMAEISGFPIGNNVIPG
                     IFTARVHAEEDNLWRTLHQSDEDTWFWERFSATSTTLPAVRTYPISLSGVAPGGGPAI
                     LSGELHVDTESPGISPDHHIELLINGVSILIAEWDGKGRYAFSAEFSPGLLVDGENQL
                     TLKAYSVPGGSGDTYFFDWFEITYPRLFSAQADQLTFHYSEMGTAFHYQIDGFTSAQI
                     GVLDISNPLEPVVLTTLAGSQQGSAYRVDFQSQHPQTGRYLVTANPLRSPLQVSSYTF
                     KNLHASTLGADYLIIAHSQFLDAAQDLADYRSSQGLRSRVIDLEDILNEFNAGIYHSL
                     AIKSFLAYTLANWAAPAPSYVVLVGSGHWNFFGRPGPTNNYSDAPIYMPPHLVWVDPW
                     QGEVDSASALAAVVGNDVLPDLAIGRIPVDSAEELSAVIQKIISYEAQPGSTDSDRLL
                     FIADNQPDDAGYFRDISEEVISTSIPADQVIDRVNLNDFLNQVDGKTCTPVPFGRDCP
                     EATQAILDAFNHLPVEFVNYTGHATANYWADEELLRTTSEPDRINDPAYNDLALLDNP
                     NYLPVLVSMTCLDGYWIYPSPRTPSLAVNLLRKAGGGVSAAYSPTGLGLANGHDILNK
                     SFFDSIYVHDIRELGAAVEYSKLKLFETGQNFDLIYTYTIFGDPALRLTTPLPSRLYF
                     PLLPARP"
     gene            2076..2588
                     /locus_tag="VN363_06285"
     CDS             2076..2588
                     /locus_tag="VN363_06285"
                     /inference="COORDINATES: protein motif:HMM:NF012920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="S24/S26 family peptidase"
                     /protein_id="HWQ84156.1"
                     /translation="MMDPYHEDQTPHPTNNLNSYRQAAIELIDQLRGEPFQLRVVSES
                     MRPFLQIGDMIWVDPRTDQVLQRGDIVVRLETGSAEGPVRDWTVHRLVRISDQGWLTK
                     GDHLRGFDPPVTREAILGRVIRIERQGLNKNLQQFPWNLAGQWLGFYHYWLGFLFVLL
                     RRGRGRVESG"
     gene            2645..2884
                     /locus_tag="VN363_06290"
     CDS             2645..2884
                     /locus_tag="VN363_06290"
                     /inference="COORDINATES: protein motif:HMM:NF017239.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PqqD family protein"
                     /protein_id="HWQ84157.1"
                     /translation="MNQVTGGEAVLVLPHAGQIKVLNGVGTFIWTALDGQQSIAEIAN
                     QMYVEYQVDIEQAQVDALEFLNDLDGRGILQVIES"
     gene            2881..>3513
                     /locus_tag="VN363_06295"
     CDS             2881..>3513
                     /locus_tag="VN363_06295"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84158.1"
                     /translation="MRIQTSFLNRLYRPGLSRAILVLAVCAALIGSFYPTTPAYSQTA
                     VTLLRLTAIGRINNILIEWETATEFNNAGFFVWASDSADGTFNPISDFIEAEGDGVTG
                     AVYGFVDSDVQAAIPKYYKLEAIELNQESEFFGPVSAASNQGLPTTTLTATATSTISA
                     PATQPAGAPTAAPTATARTSSNPTAATPASGNESAYPGQTTPTPYIQPAFN"
BASE COUNT          789 a          922 c          936 g          866 t
ORIGIN      
        1 gccgcatatg gctgctgcca tgtttgaaaa atacactgat caaaacgtat actggctgga
       61 tatcgatgga cctgccgggc tgcggatggc tgaaatcagt gggttcccga ttgggaacaa
      121 cgtgatcccc ggaatcttca cagcccgggt ccatgcggag gaggataatc tctggcgcac
      181 gctccatcaa tccgatgagg atacctggtt ttgggaaagg ttttctgcca cctcgacaac
      241 ccttcccgcc gtgcggacat atccaatcag tctctcaggc gtcgctcctg ggggtggtcc
      301 tgcaatcctg agtggcgagc tgcatgtcga tacagaaagt cccgggatat caccggacca
      361 tcatattgag ctcttgatca atggcgtttc gatcctgatt gccgagtggg atggcaaggg
      421 tcgctacgct ttttcggcgg aattcagccc tggcctcctg gttgacgggg agaaccaact
      481 cactttaaaa gcttattcag tcccaggtgg atctggtgat acttattttt ttgattggtt
      541 tgagatcacc tatccccgcc tgttttcagc tcaggctgac cagctcactt ttcattattc
      601 agagatgggg acagcttttc attatcagat agatggtttt acctctgccc agatcggtgt
      661 actggatatt tccaatccgc tggagccggt tgttcttaca accttagccg gaagccagca
      721 agggagcgcc tatcgagtcg atttccagtc ccagcaccct cagactgggc gctacctggt
      781 cactgccaat cccctccgat cgccgctgca ggtttcaagc tacaccttta aaaacctgca
      841 tgccagcact ttgggagcag attatctgat catcgcccat tcccagtttc tggatgccgc
      901 tcaggatctg gcagattatc gttcttccca gggtctgcgc agccgggtca tcgatctgga
      961 agatatcctc aacgagttca atgccgggat ttaccattcc ctggcgatca aaagcttcct
     1021 ggcgtacacg ctggcaaatt gggcagcccc ggccccttcc tatgtcgttc tggttggtag
     1081 cgggcattgg aatttctttg gtcggcctgg accgaccaat aattactcgg acgcacccat
     1141 ttacatgccg ccccatctgg tctgggtgga tccctggcag ggcgaggtcg acagcgcctc
     1201 agccctggca gcagtggtag ggaatgatgt cttaccggac ctggcgattg gtcgcatccc
     1261 ggtggattca gcggaagaat tatcggcagt catccagaaa atcatctcct acgaggcaca
     1321 accgggttcc actgatagtg accgcttact tttcattgcc gataatcagc ccgatgatgc
     1381 gggttatttc agggatattt ctgaggaggt catctctacc agcattcccg ctgaccaggt
     1441 gatcgaccgc gttaacttga atgattttct taaccaggtc gatggaaaaa cctgtacacc
     1501 ggttccattt ggaagagact gtcccgaagc aactcaagcg attttagatg cgtttaatca
     1561 cctgccggtt gagtttgtta actataccgg acatgccacc gctaattatt gggcagacga
     1621 agagctttta cgaacaacca gcgagccaga tcggataaac gacccggcat ataatgatct
     1681 ggccttactc gataatccca attacctgcc ggtcctggta tcgatgacct gcctggatgg
     1741 ttattggatc tatccctcgc cgagaactcc cagcctggca gtcaacctgc tgcgaaaggc
     1801 gggaggagga gtatccgcag cctattctcc caccgggctg ggtctggcga acgggcatga
     1861 tatattgaat aagtctttct tcgattcaat ctatgtccat gatatcaggg agcttggggc
     1921 ggcagtcgag tacagcaagc tcaagctttt cgaaaccggc cagaattttg acttgattta
     1981 tacctacacc atcttcggtg accctgccct gcggttaaca acacctcttc caagcaggct
     2041 gtatttcccg ttattacctg ccagaccata atcagatgat ggacccgtat cacgaggatc
     2101 agacacccca tccgaccaat aatctgaaca gttatcgcca ggctgccatc gagctgatcg
     2161 atcagctgcg cggcgaaccc ttccaattgc gggtagtcag cgaaagtatg cggcccttct
     2221 tgcaaattgg ggatatgatc tgggtggatc cgaggaccga tcaggtgctt caacgaggcg
     2281 atattgtcgt ccggctcgaa actggctcgg cggaaggtcc ggtccgagat tggaccgtcc
     2341 accgcctggt caggatttcc gatcaaggct ggctgaccaa aggggatcat ttgcgcggct
     2401 ttgatccgcc ggtcacccgg gaggccattc tgggccgggt gattcgcatt gagcgccagg
     2461 gtttaaacaa aaatttacag caatttccat ggaatctggc tggtcaatgg ctgggtttct
     2521 atcattactg gcttggtttc ctgtttgtgc tcttgcgcag aggccggggt cgggtagaat
     2581 caggctgagg cacacacaga cgatgttaac gcttacgagt gtatgtaaac cgaagccggg
     2641 ggttgtgaat caggttaccg gtggcgaggc agtcctggtg ctgccgcatg ccggtcagat
     2701 taaggtcctg aatggagtgg ggactttcat ctggacagct ctggatggac agcagtcgat
     2761 cgcggagatt gccaatcaga tgtatgtaga gtatcaggtg gatatcgaac aggcgcaggt
     2821 agatgcgctc gagttcttga atgacctgga tgggcggggt atcctgcagg tgattgagtc
     2881 ttgagaattc aaacgagttt cctgaacagg ctttacagac cgggattgtc cagggctatc
     2941 ctggttctgg ctgtctgtgc agctctgatc gggtcgtttt acccgaccac gcctgcctac
     3001 agccagactg cggtaacttt actgcgatta accgccatcg gacggataaa taatattttg
     3061 atcgaatggg agactgctac cgaatttaat aatgccggct ttttcgtatg ggccagtgat
     3121 tcagccgatg gtacgtttaa tccaatcagc gattttattg aggcggaggg ggatggtgtg
     3181 accggcgccg tgtatggatt tgtcgatagc gatgtccagg cagccattcc caagtattac
     3241 aaattggagg ctatcgagct gaaccaggaa agtgagtttt ttgggccagt ttcggcagcc
     3301 tccaaccagg gtctgccgac gacaaccctg actgcaacgg caacttccac aatatctgca
     3361 cctgcgacac agcctgcagg tgcccccacg gctgcgccaa ccgcaacggc gagaacaagc
     3421 tcaaatccaa ccgcagccac acccgcttct ggaaatgaat ctgcatatcc ggggcaaaca
     3481 accccaacgc catatatcca gcctgctttc aat
//
LOCUS       DATMXK010000342         4797 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_166448, whole genome shotgun sequence.
ACCESSION   DATMXK010000342 DATMXK010000000
VERSION     DATMXK010000342.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4797)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4797)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4797
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166448"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(85..726)
                     /locus_tag="VN363_06300"
     CDS             complement(85..726)
                     /locus_tag="VN363_06300"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ84159.1"
                     /translation="MLENIRILLVDDHAVMRSSLGMLLESYGARIVGEAATGEEAIAQ
                     ALALKPDVILMDITLPGMDGIEATRRISTDWPEARILALTMHTEEIYLSPFLEAGGAG
                     YIRKSAADRDVLDAIQKVLQGQMLLDTQSVQELLKRGKKSTASRDLDAALSEREREVL
                     VRVARGYTSFEIGEQLNISPRTVDTYRSRIMQKLGLEHRHQLVEYAVKNRLLE"
     gene            complement(731..1897)
                     /locus_tag="VN363_06305"
     CDS             complement(731..1897)
                     /locus_tag="VN363_06305"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sensor histidine kinase"
                     /protein_id="HWQ84160.1"
                     /translation="MDRKHTPLPRLFRLLWGRIISAPVTIKIVGLAILPVATIILAAW
                     LYSEIFIFRPLHANPDIALPVQDLYTLILRAFFALGAGALVGIGASALLTWVLIRQMS
                     GLVETMGRVEAGDLTVRSPVWAQDEIGKVQAAFNTMVNSLQQSRDALVKNQQELELLN
                     AENNRLITETRQKEAELRLALKRAVDSQEEERKRISRELHDEIGQALTSILIRLKSLQ
                     DVTDLEVITDRMNGIRYLASQAIEEMRRLSMDLRPAALDNLGIIPALRWYIGQSMEQN
                     QIEIQFIAPERMERLPPEVEIVLYRVAQEGLNNAMRHSQAKRIEVKLDLTPRFIWLSI
                     SDNGQGFDADTLHRGLGLVGVRERVELLKGQCRIDTQRGNGTRLWVEIPLAEGT"
     gene            complement(2513..3103)
                     /locus_tag="VN363_06310"
     CDS             complement(2513..3103)
                     /locus_tag="VN363_06310"
                     /inference="COORDINATES: protein motif:HMM:NF024751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWQ84161.1"
                     /translation="MVGGVLRRLRVRPCATGLWGNLFVQSHFNWYYQSINQFSNSIIR
                     TGDQMTVNIRCDRCSGMTKSSRSEDGWYKRNGTRNLFMLVEPKAGFRHVLITRRRTKL
                     EFAYAMPYLVDVLYPDTEWIDVVLDCLNTHQEVVLIEIFGKTEADQILSRIRFHHPPV
                     HASWLNMAEIEIAVLEEPCLNRRLSDEFTLGAEVVA"
     gene            complement(3166..3238)
                     /locus_tag="VN363_06315"
     tRNA            complement(3166..3238)
                     /locus_tag="VN363_06315"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(3203..3205),aa:Ala,seq:cgc)
     gene            complement(3306..3959)
                     /locus_tag="VN363_06320"
     CDS             complement(3306..3959)
                     /locus_tag="VN363_06320"
                     /inference="COORDINATES: protein motif:HMM:NF014269.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LON peptidase substrate-binding
                     domain-containing protein"
                     /protein_id="HWQ84162.1"
                     /translation="MYELALFPLNTVLYPGTPLQLHIFEERYQQMVGDCLAEEKPFGV
                     VLIRRGVEALGALADPYRIGCTASIVDVERLADGRMNITAIGKDRFRILTLDSLSHPY
                     LVGMVENYPIKADSLDELQPGAAQLLARLEVYLELLVEAGLIELDKSHLPAEPLSLAY
                     LAAAVLQVPPIQKQQLLSTHTAQSLIDRLNNIFKREVALTRQLVNKNGSQQGPFSVN"
     gene            4136..>4797
                     /locus_tag="VN363_06325"
     CDS             4136..>4797
                     /locus_tag="VN363_06325"
                     /inference="COORDINATES: protein motif:HMM:NF013486.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminopeptidase P family N-terminal
                     domain-containing protein"
                     /protein_id="HWQ84163.1"
                     /translation="MNSKTSSEVGNRILSRQARLFPLLPQDGLDALVLNPGPTLSYLT
                     GLSFHLSERPVVLILAPTDSPVLILPELEAGKIDTLLFPIRSFAYDEDPDSWPDVFKQ
                     AVRAAHIQRSTVGVESGRMRFLELSLLQTASKDIRFEAADPTLARLRMQKDPYEIDQM
                     RTAVQIAQKALQVTLSTIKAGLSEREIAAELTTQIYRSGSDSELPFAPIVSAGPNSAN
                     PHA"
BASE COUNT         1147 a         1255 c         1222 g         1173 t
ORIGIN      
        1 gaaaatggga acgatgcaga gtaaacccca cttgatttca agctattgac ttttccgaaa
       61 ttgaaaggag agttgaaatt aatgtcactc cagcaggcgg ttcttcaccg catactccac
      121 gagttgatgg cggtgttcca gtcccagttt ttgcataatg cgggagcgat atgtatccac
      181 agtgcgcgga ctgatgttca gttgttcccc gatctcaaaa ctggtgtaac cgcgcgcgac
      241 gcgaaccagt acttcccgct cacgttccga gagggcggca tccaagtctc ttgacgccgt
      301 tgatttcttg ccgcgtttta acaattcctg tacgctctgt gtatcgagaa gcatttggcc
      361 ttgaagaact ttttggatgg catccagtac gtctcggtcg gcggcagatt tacggatgta
      421 accggccccg cccgcttcga gaaaaggcga caggtatatc tcctcggtat gcatggtcaa
      481 ggctagaata cgcgcctcgg gccagtccgt ggagatgcgc cgtgtcgctt caatgccgtc
      541 catgccaggc agagtaatgt ccatcaggat cacatcgggt ttgagcgcca gcgcctgggc
      601 aatggcttcc tctccagtcg ccgcctcccc aacgatacgc gcgccatagg attcgagcaa
      661 cattcccaga ctgctgcgca ttacggcgtg atcgtctacg agcaggatgc ggatattttc
      721 aagcatggat ctatgtcccc tccgcaagtg gaatttcaac ccacaggcgg gtgccgttgc
      781 cacgctgcgt gtcgatcctg cattgaccct taagcagttc cacgcgttcg cggactccga
      841 ccagtcccaa gccgcgatgc agtgtgtccg catcaaagcc ctgaccgttg tcgctgatgc
      901 tgagccagat gaagcgaggc gtaagatcca gtttcacctc gatgcgcttc gcctgactgt
      961 gacgcatggc gttgttcaat ccctcctgcg cgacgcggta cagcacaatt tccacttcgg
     1021 gcggtaggcg ctccatgcgt tccggcgcga taaattgaat ctcgatttgg ttttgctcca
     1081 tgctttgccc gatataccag cgcagggcgg ggatgatgcc caggttatcc aacgcggcag
     1141 gccgcaggtc catggagagg cggcgcatct cttcgatcgc ctgcgatgcc aggtagcgga
     1201 tgccgttcat gcggtcggtg ataacttcaa gatcggtcac atcctgaaga ctttttagac
     1261 ggatcaatat ggaagtgagg gcttgcccaa tttcatcatg caattcgcgg gagatgcgtt
     1321 ttcgctcttc ctcctgagag tccacagcgc gcttgagcgc gaggcgcaat tcggcttctt
     1381 tttgtctcgt ttctgtaatc aggcgattgt tctccgcatt cagcaactcc agttcttgct
     1441 gatttttcac cagagcatcc cgcgattgtt gcaggctatt gaccatcgtg ttgaaggcgg
     1501 cctgcacctt gccgatttca tcctgcgccc agacaggtga gcgcacagtc agatcgcctg
     1561 cttccacgcg ccccattgtc tcgactaacc cgctcatctg acgaattaag acccaggtta
     1621 gcagggcaga agcgccgatg ccgaccaacg ctcctgcgcc gagggcgaaa aatgcccgca
     1681 aaatgagagt gtacaagtcc tgaacaggca gcgcgatgtc gggattcgcg tgcagaggcc
     1741 tgaatataaa aatttctgaa tacaaccagg ccgccaaaat aatcgtcgct actggcagga
     1801 tcgccagacc tacgatcttt atggtgacgg gtgctgaaat gattcgtccc cacaagagac
     1861 gaaaaagtct ggggagggga gtgtgttttc tgtccatgaa ggaaatatta tagcgtctat
     1921 tttctgaaag cgcatttcac atttccaaag ttagaacatc cactctcggt tcgggcattt
     1981 ccaaagtctg ttcaaaacat ttgccacccg atatggcaaa aaagcgttta ctggagattt
     2041 ttgggcatgt ctggcttacc acaaatactg gacagaccga gcagtcgcag atgatgaatc
     2101 aaaacttggt aaagcatccc gtatatatgg cggttatcaa gtatgattgg atgcatatag
     2161 cgcagatgaa tcttttgaac cttggtttta aattaactaa ccgacagatt catataacgg
     2221 atacagggaa agggaagcgg taatgcaagt tctaaaaatc aaaaccgttt gagaaatttt
     2281 tcacttgaca gattcgatca tgttgtgtaa ttatcccgac aatattttgg tttctttatg
     2341 cggatttcgg tgaaatcgca cccattaaac ggagcatgcc gcacagttag gggaggaaaa
     2401 cactagtacc ttaacttgat gaatatgatt gatccttaca aaagactttt tcggccttat
     2461 gacgagtgaa agtccaatgg atcttggcct gctgttggtt tcggcgatct tctcaggcga
     2521 ctacttccgc gccaagtgtg aattcatctg agagtctccg attcaggcat ggttcttcca
     2581 atacagcgat ctcgatctcg gccatgttga gccaggaagc atgaactggc ggatggtgaa
     2641 agcggatccg gcttagaatt tgatctgctt cggtttttcc aaagatctcg atcaagacga
     2701 cttcttgatg ggtgttcaag caatccagaa cgacatcaat ccactcagta tcgggataaa
     2761 ggacatccac caggtatggc atagcataag cgaattcgag tttggttcgc cgacgagtaa
     2821 tcaggacatg tcgaaacccg gcttttggct caaccagcat gaacagattg cgagtgccat
     2881 tgcgtttgta ccacccatct tcgcttctcg acgacttggt catgccagag catcgatcac
     2941 atcggatatt tacggtcatc tgatccccag tgcggatgat ggagttgctg aactgattga
     3001 tcgactggta ataccaattg aagtgacttt ggacgaagag atttccccat aaacccgttg
     3061 cacacggtcg cacacgcagc cgtcgaagaa ctcccccaac cataaccctt accacatttt
     3121 tggttcattt aagccaaaaa cccctaatat atcaggggtt ttcagtggac ctggagggat
     3181 tcgaaccctc ggcctcttca gtgcgattga agcgcgctcc caactgctct acaggcccgt
     3241 gccatacgac agcatagaat tgtaccaaat gcggataccc tgtcaaggac ggaattggcc
     3301 agggatcaat tgacagagaa tgggccttgc tgactcccat tcttgtttac cagctggcga
     3361 gtcaatgcca cttcgcgttt aaaaatatta ttcaagcggt cgatcaaaga ttgtgctgtg
     3421 tgtgtcgaaa gcagctgttg tttctggatc ggtggtacct gaagtactgc agcagccagg
     3481 taggccaagg ataatggttc tgcaggcagg tggcttttat ccagctcaat cagtccggct
     3541 tccaccagaa gttcaaggta aacttccagg cgagcaagca attgggccgc gccaggctgc
     3601 agctcatcca gggagtctgc cttgatcgga tagttttcta ccatgccaac cagatagggg
     3661 tgcgagagac tgtccagggt gagaatccgg aagcggtctt tgccaatcgc ggtgatattc
     3721 attctcccat ctgcgaggcg ctccacatcg acaatactgg ctgtacagcc tatccggtaa
     3781 ggatcagcga gggcacccag agcttcgacc ccccggcgga tcaacacaac gccaaaaggt
     3841 ttttcttcgg cgaggcaatc tccgaccatc tgctggtagc gctcctcaaa gatatgcagc
     3901 tgcaaaggtg taccagggta taaaaccgta ttgagcggga agagagctaa ttcatacatc
     3961 gtctaacctg accgacatga tcacaggcaa ggtaacttat tctatcatgc cctggttgcc
     4021 aggcagtctc cttcctcagc ttatgtaaat gaatcgctac caggctgatt gggttcaggc
     4081 tataatgaac cacctattcc agatcaattt cccatccggc aaggtgaggt gctgaatgaa
     4141 ttctaaaacg agctcggagg tcggcaaccg aattctttcc cgccaggcac gcctgttccc
     4201 tctgctcccg caggatggcc tggatgcgct ggtgctaaat cccggtccga cgctatctta
     4261 tctgaccggc ttgagcttcc atcttagcga acggcctgtt gttttaatcc tcgctccaac
     4321 cgactcaccc gtgctgatcc tgcctgagct agaagctggc aaaatcgaca ccctgctgtt
     4381 tccaatccgg tcatttgcgt atgatgaaga tcccgattca tggccggatg tatttaaaca
     4441 ggcagtccgc gccgcgcata tccaacggag tacggtgggc gtagagtctg ggcggatgcg
     4501 ctttttggag ctgagcctgc ttcagacagc cagtaaggat attcgctttg aagccgccga
     4561 tcctaccctg gcgcgtttac ggatgcaaaa ggatccgtat gaaattgacc agatgaggac
     4621 cgcggttcag attgctcaaa aggctctcca ggtgaccctg agcacgatta aagctggcct
     4681 gagcgagcgc gagatcgcag cagagctgac aacccagatc taccgcagtg gctcagattc
     4741 tgagctgccc tttgcgccaa tcgtttcggc tggcccaaac agcgccaatc cgcatgc
//
LOCUS       DATMXK010000343         3257 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_160104, whole genome shotgun sequence.
ACCESSION   DATMXK010000343 DATMXK010000000
VERSION     DATMXK010000343.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3257)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3257)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3257
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160104"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..873)
                     /locus_tag="VN363_06330"
     CDS             complement(<1..873)
                     /locus_tag="VN363_06330"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561526.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent epimerase/dehydratase family
                     protein"
                     /protein_id="HWQ84164.1"
                     /translation="MSTYLVTGAAGFIASRVAGLLLEAGHDVFGVDNLNDAYDVRIKH
                     WRLNRLNQQANFRFAELDISQHEVLKDAVSSDLAERGQSTYDAIFNLAARAGVRQSVL
                     NPWVYVDTNVTGTLNLLELCNQIKIPKFILASTSSIYGANAPLPTSEEADSDHPLQPY
                     SATKKGAEALCHAYHYLNGIDVTVFRYFTVYGPAARPDMVMFRFVQWIKEGRPVYVNG
                     DGEQSRGFTYLDDIARGTILGLKPLGYEIINLGGHEVIKINELIQILEGMLGQKARLE
                     YLPRHPADMLTNWAL"
     gene            complement(949..2271)
                     /locus_tag="VN363_06335"
     CDS             complement(949..2271)
                     /locus_tag="VN363_06335"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561527.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWQ84165.1"
                     /translation="MSTETQLALRKKLEDRTAQIAILGLGYVGLPLAVVFAESGFTVT
                     GIDPDRRKVDTVQRGESHIQDISTEQVARLVQAGKLKATTDFAALREADAVSICVPTP
                     LRKTGDPDLSYILSATEELAKYVHPGMVVVLESTTYPGTTREVLLPKLEEVPGIKVGE
                     NFFLAFSPERVDPGREDWTTLNTPKVVGGITQNCSDISAVWYSQALETVVPVSSAEVA
                     EMAKLLENTFRMINIGLVNEMAIMCTRLGVDVWEVIDAAATKPFGFMKFTPGPGLGGH
                     CIPIDPLYLSWKLRSLNYTARFIELASEINTGMPRYVVGLVQDALNDQSKSIKGSNVL
                     VLGAAYKPDIDDLRESPALDVIGLLEQKGAHVSYHDPYVPCIAHEHWTRESVPDLMAA
                     VHTADCVVVVTNHTVYDYPAILEEATLIVDTRNAYGKIAHNNPKVVRL"
     gene            complement(2340..2978)
                     /locus_tag="VN363_06340"
     CDS             complement(2340..2978)
                     /locus_tag="VN363_06340"
                     /EC_number="3.2.2.27"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561528.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004844 - uracil DNA N-glycosylase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uracil-DNA glycosylase"
                     /protein_id="HWQ84166.1"
                     /translation="MTDSVKELKQVADEVVDCTMCKLHHSRKLAVPGEGPVNASIMFI
                     GEGPGFHENEQGRPFVGAAGKLLEELLGNIGMQRNQVFITNIVKCRPPGNRDPEPEEI
                     LACNNYLERQIKAINPKVVVTLGRYSMGKFMPNAKISVVHGQAKQINGRLIIAMYHPA
                     AALHQRSLLSVIQDDFAHLPELLAQADQIEDPSEESKPDPSPKNPPTQLSLF"
     gene            complement(3005..>3257)
                     /locus_tag="VN363_06345"
     CDS             complement(3005..>3257)
                     /locus_tag="VN363_06345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84167.1"
                     /translation="LLVQALQDSIQHNCTSATLEVRAGNLAAQGLYQGFGFEIVGMRP
                     KYYQDNQEDALIMTVSPLNMAYNEGLKSISNQPEVIDLD"
BASE COUNT          752 a          822 c          858 g          825 t
ORIGIN      
        1 cagggcccag ttggtgagca tatctgcggg gtggcggggc aggtattcta gccgggcttt
       61 ctgacccagc atcccttcca gaatctggat cagctcattg atcttgatta cttcatgccc
      121 tcccaggttg atgatctcat aacccaaggg tttcagcccg aggatcgtgc cgcgggcaat
      181 atcatccagg taagtaaagc cgcgcgactg ttcgccatca ccattgacat aaaccggccg
      241 gccctccttg atccattgca cgaaccggaa cataaccatg tcaggacgcg ccgcaggtcc
      301 atagactgta aaataccgga atacagtcac atcgatgcca ttgaggtagt gataggcatg
      361 gcaaagcgct tcagctcctt ttttggtagc cgaatacggc tgcaggggat ggtcgctgtc
      421 tgcttcctct gaagtaggca ggggagcatt cgcgccataa atacttgaag tggaggccag
      481 gataaatttt gggatcttaa tctggttgca taactcgagt aagttcagcg tgccggtgac
      541 attcgtatcc acatacaccc atggattcaa cacagattgg cgaacacctg cccgggcagc
      601 cagattgaag attgcgtcat acgtggactg gccgcgttcg gccagatctg agctcactgc
      661 atctttaagg acctcgtgct ggctgatatc cagctcggcg aagcgaaaat tagcttgctg
      721 attaagccgg tttaatcgcc agtgctttat gcgcacgtca taggcatcgt taagattgtc
      781 cacaccaaaa acatcgtgcc cggcctccaa cagaagacca gccacacgcg aggcgatgaa
      841 acctgctgcg ccagtcacaa gataagtact cattctattc tccgatctgg agttgttgat
      901 taaaagagtg agtcgccggg ttaaagcgac tcacgcgaat tattttggtt aaaggcgcac
      961 aaccttggga ttgttatggg cgatcttccc ataggcattg cgagtatcga cgatcagtgt
     1021 agcttcttcc aggatggcag gataatcata gactgtatgg tttgtgacca cgacaacaca
     1081 atcagcagta tgcactgctg ccatcagatc tggcacactt tcacgcgtcc agtgttcgtg
     1141 tgcgatgcaa ggaacatagg gatcgtggta agaaacatgg gcaccttttt gttccagaag
     1201 accgataaca tccagagcag gtgattcgcg caggtcgtcg atatccggtt tgtaggcagc
     1261 tcccaggaca aggacattgc tgcccttgat cgacttggat tgatcgttta atgcatcctg
     1321 caccagtccg accacgtagc gcggcatacc ggtattgatc tcggaagcca gctcgataaa
     1381 tcgggctgtg taattcaggg agcgcagttt ccaggacagg tatagtggat caatcgggat
     1441 gcagtgccca cccaggccag gacccggtgt aaatttcata aaaccaaacg gcttggttgc
     1501 ggcggcgtcg atgacttccc agacatccac acccaggcgg gtgcacatga tcgccatttc
     1561 gttgaccagg ccaatgttga tcatccggaa agtattctct aagagttttg ccatctcggc
     1621 aacctcggcg gacgaaaccg gtacaactgt ctctagagcc tgtgaatacc acaccgccga
     1681 gatatcggag cagttttgag taattccccc gacgactttg ggagtattta atgtagtcca
     1741 gtcttcgcgc ccgggatcga ctcgctcggg cgaaaatgcc aggaagaagt tttccccgac
     1801 cttaatgcct ggcacttcct ccagttttgg aagcagaact tcgcgtgtgg ttccgggata
     1861 agttgtggat tccagaacaa ccaccatgcc tggatgaacg tattttgcca gctcctcggt
     1921 cgcggataat atgtaggaaa gatccggatc gcctgttttg cgcagtgggg tgggaacaca
     1981 gatgctgacc gcatctgcct cgcggagtgc ggcaaagtcg gtggtggctt ttagctttcc
     2041 tgcctggacc aggcgagcta cctgttcagt agaaatgtct tgaatatggc tttcaccacg
     2101 ttgaaccgta tctacttttc gccgatcggg atcgatcccc gtaacggtaa aacctgattc
     2161 agcaaagacg actgccagag gcaaaccaac gtaacccagg cccaggattg ctatctgtgc
     2221 agttcggtct tcaagtttct tgcggagtgc gagttgtgtt tcggtgctca ttgatcggtg
     2281 tcctctactt agggatattc tcggttattt cgggagattc actcgacttt tcgctgtcct
     2341 taaaacaggc tcagctgggt cggtggattt ttgggagatg gatcaggttt tgattcttcg
     2401 gatgggtctt cgatttgatc cgcctgagcg agaagctcgg gtaggtgagc aaaatcatcc
     2461 tggattaccg agagcagcga gcgctggtgg agcgctgcag cgggatggta catggcgata
     2521 attaaccgcc cattgatttg tttggcctgt ccatgtacca cgctaatttt tgcattaggc
     2581 ataaacttgc ccatcgagta gcgccccagg gtgacaacga ctttagggtt aatcgccttg
     2641 atctggcgtt caaggtagtt gttacaggcc aggatttctt caggttcagg gtcacggttg
     2701 ccaggtggcc ggcatttgac gatattggtg atgaaaacct ggttgcgctg cataccgata
     2761 ttaccgagca gctcctccag cagtttaccg gctgcgccaa caaatggacg gccttgttcg
     2821 ttttcatgaa aaccgggtcc ttcgccaatg aacatgatcg atgcattcac tggaccttcg
     2881 ccagggacag ccagttttcg ggaatggtgc agcttgcaca ttgtgcaatc tacgacctca
     2941 tctgcaacct gttttaattc cttaactgag tccgtcaatc tgtactcctg atattctgtg
     3001 gcggttagtc cagatcgatc acctctggtt gattcgaaat cgatttcagc ccctcattgt
     3061 acgccatatt taatggagat acggtcatga tcaaagcatc ttcctggttg tcctggtaat
     3121 attttggccg catcccgact atctcaaagc cgaagccctg gtataagcct tgtgcggcca
     3181 gattcccggc ccggacttca agggtggcgc tggtgcagtt atgctggata ctatcctgca
     3241 aagcctgcac cagtaac
//
LOCUS       DATMXK010000344         2188 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_67723, whole genome shotgun sequence.
ACCESSION   DATMXK010000344 DATMXK010000000
VERSION     DATMXK010000344.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2188)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2188)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2188
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67723"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(93..668)
                     /locus_tag="VN363_06350"
     CDS             complement(93..668)
                     /locus_tag="VN363_06350"
                     /inference="COORDINATES: protein motif:HMM:NF013122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008408 - 3'-5' exonuclease activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'-5' exonuclease"
                     /protein_id="HWQ84168.1"
                     /translation="MVTSIQARRMAILKAQEYLRARPLYLDTETTGLNPLDEIVEICI
                     VDHDGKVLLNSLVRPTRPIPAEVIRVHGITNPMVAGAPAWSEVWPEVRAILSGRQVGI
                     YNADFDLKMMQQSSRAHRLTLDQTGWDRFCLMKLYASYYGQVGYRGEFRWQRLEDAAR
                     QCQIKLLNTHRALDDTLLARALLEYMASQTP"
     gene            818..>2188
                     /locus_tag="VN363_06355"
     CDS             818..>2188
                     /locus_tag="VN363_06355"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWQ84169.1"
                     /translation="MIPGLVMVVSWLTGWFALARLGVPAIIKLAIVTILSGLAAGLVF
                     EMIRLAHMQKSAEKNIQRLSHRADQLTNQMAGVYALSSLYAKPSTEKEIITALLKLCI
                     DLVGARGASFVPLDDQGQPMAAQTQGELPFPALDAWVEYLASPAIRQRCEQCQSYGHL
                     NTSCPLLKSPITDVIGLFCLPLNSGNREWGIMNLYMPNHDRLDPDTEIFVKTLLQDTA
                     LALESRRLRQREFDAIQQLRLIQQRTDLSGLLTSLLDNIRMAFGAELAQVELTLRRDR
                     RRLSAGETNKIFESEINQIVERILLTGEAVEFQTTNQWHQSSFSEFSILAVPLLSSQK
                     QVKGTLLVVTPPILQNQTHQLSLLQAFADQVELVIENAELLAHIEYQTVIQERTRLAR
                     EIHDGLAQTVGLLKLQTAQSLNYLSKADTEKLRFTLTMVHNTLSEAYQDIRSTIDGLR
                     IDTDPHG"
BASE COUNT          537 a          587 c          559 g          505 t
ORIGIN      
        1 agcattaacc gctgatcacc agaaaataaa aatcccctgc tggaccatca atagttggat
       61 tccggcaggg gagtaaccat agctcaagaa tttcagggtg tctggctggc catatactcc
      121 agcagcgccc gcgccaggag ggtatcatcc agggcgcgat gcgtatttag cagcttaatc
      181 tggcattggc gggcagcatc ttcgaggcgc tgccagcgaa actcaccgcg atatcctacc
      241 tgcccataat atgaagcgta aagcttcatt aaacagaacc tgtcccatcc ggtctggtcc
      301 agggtcaggc gatgtgcccg gctggactgc tgcatcatct tgagatcaaa gtcggcgtta
      361 tagattccca cctgcctgcc agataagatc gcccgaactt caggccagac ttcggaccat
      421 gcgggtgcac cggcaaccat cggattggtt atgccatgca cacggattac ctccgccggg
      481 attgggcgtg tgggacgcac caggctgttc agcagcacct tcccatcatg gtctacaatg
      541 caaatttcga caatttcatc gagcggattc aacccggtgg tttcggtatc cagataaagc
      601 ggcctggcac gtaaatattc ttgagcttta aggatcgcca tccggcgggc ctgaatagat
      661 gtaaccatac atgctcccag caaaggtgat gaataaatct taccactatc accggattgc
      721 ggtatggcgt ttgtgaagtg gaccgattaa gggtaaaata ggcgcgatgc tattgaaacg
      781 attatctcac ccaggcatcc tgggtctaag agggtggttg atcccaggcc tggtaatggt
      841 ggtgagctgg ctgactggct ggtttgccct cgccaggctc ggcgtgccag cgattattaa
      901 gctcgcaatc gtaaccattc tgtctggttt ggcagccgga ctggtttttg agatgattcg
      961 actggctcat atgcagaaga gcgctgaaaa gaatatccag cggctttccc accgggcaga
     1021 tcaattaacc aaccagatgg ccggcgtata tgctttgagc tcgctatatg cgaagcccag
     1081 cactgaaaaa gaaatcatca cggctttatt gaagttatgt atcgatctgg taggtgcccg
     1141 gggagcttct tttgttccgc tggatgacca gggccaaccg atggctgccc aaacccaggg
     1201 tgaattgcct ttcccggcat tagatgcctg ggtagaatac cttgccagtc cggcgatccg
     1261 gcagcgttgc gaacaatgcc agtcctatgg gcatttgaac actagctgcc cgcttctcaa
     1321 aagtcccatc accgatgtaa ttggcctgtt ctgcctcccc ctcaattctg ggaatcgtga
     1381 gtgggggatc atgaacttat acatgccaaa ccatgatcgg ctggaccctg atacagaaat
     1441 tttcgtgaag acattgctcc aggacactgc cttagctctg gaatcgagac gcttgcggca
     1501 gcgcgaattt gatgcaatcc agcagcttcg cctgatccag cagcggaccg atctttctgg
     1561 actcctgacc tccctgcttg ataatatccg aatggccttt ggcgccgagc tggcgcaagt
     1621 tgaacttacc ctcaggcgtg atcggcgcag gttatctgcc ggggaaacca ataaaatttt
     1681 tgaatctgag atcaaccaga tcgtagagag gatcctactc actggtgaag ccgttgaatt
     1741 tcaaacaacc aaccagtggc atcagagcag tttttctgag tttagtatcc tggctgtgcc
     1801 attgctctcc agccagaaac aggtcaaagg cactcttctg gtggtgacgc cgcccatcct
     1861 ccaaaaccag actcaccagc tcagcctgct gcaagctttc gccgaccagg tggagctggt
     1921 aatcgaaaac gccgagctat tggcccacat cgagtatcaa acagtaattc aggaacggac
     1981 gcgactggca cgtgaaatcc atgatggact ggcgcaaacg gtgggactgc taaaacttca
     2041 aaccgcccaa tccttgaatt acctcagtaa agcggatact gagaagctgc gctttacatt
     2101 aaccatggtt cacaacacct taagcgaagc ctaccaggac atccgctcta ctatcgatgg
     2161 cttgcggatc gataccgacc cgcacggc
//
LOCUS       DATMXK010000345         3138 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_5478, whole genome shotgun sequence.
ACCESSION   DATMXK010000345 DATMXK010000000
VERSION     DATMXK010000345.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3138)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3138)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3138
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5478"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..516
                     /locus_tag="VN363_06360"
     CDS             <1..516
                     /locus_tag="VN363_06360"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558968.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="magnesium chelatase"
                     /protein_id="HWQ84170.1"
                     /translation="QLLDRFALSVEIHGIRDARDRVLIMERNLAFEGDPESFQQDWLP
                     REQELSQQIEHARQVLDQVTHTSRDLLAIAALTSSLNVDGHRSDLVILKTARAHAAFE
                     NRRTIVDRDIALAAELALPHRIKRGPFHQAEITMEQLQERIEQLEGAIDHSQETETQP
                     VQEPSSEKKKV"
     gene            651..1472
                     /locus_tag="VN363_06365"
     CDS             651..1472
                     /locus_tag="VN363_06365"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558969.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWQ84171.1"
                     /translation="MPRRLANPLDRITRRHGGRRSQTHTDRKRGHYIQARPAMGKTND
                     LAFDATLRAAAPYQRERLEQRKQVAFAVQKSDFQRKVRVTKAANLVLFVVDASWSMAV
                     AERMQATKGAILSLLTDAYQRRDRVGLVVFQKDRATLLLSPTNSIQLAQRSLADIPVG
                     GKTPLSAGLLLAHQVIQRERIMHPDVMPLIILLTDGAGNVSLSNLPPQEESHRLADQI
                     SDDEIRCVVINMEHAAFDQGLAQALADHLKSPCYSLNELKAETLYRAVRDEMLGA"
     gene            1482..3041
                     /locus_tag="VN363_06370"
     CDS             1482..3041
                     /locus_tag="VN363_06370"
                     /EC_number="3.4.11.-"
                     /inference="COORDINATES: protein motif:HMM:NF013592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004177 - aminopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008237 - metallopeptidase activity
                     [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="M1 family metallopeptidase"
                     /protein_id="HWQ84172.1"
                     /translation="MPALLWSPFHRDNRPVIQTVVLLAFLVAACTANSRLPDASPPHT
                     VTPEILPAETNNTPTSPSGIQPTPAGAAATVDSISQPTPTTSAPVPLPHYTLTATLNY
                     AAHFLQVDERIDYTNSSADTLTELVLMVDAVYFPGTFTLKSLTWVDSEANPEVTWDNA
                     ILHLQLPQPLPPGEQVSLSLSYTLNLPSPVPSEQVRPIPFGYTARQTNLVDWYPFIAP
                     YISGEGWIANQAGFFGEHLAYAVSDFEISIRLEDALTGLIVAASSPAEEVDGWLHYQM
                     EKARSFAWSVSHDYIVAATQVGETTVTSYTFPVHAAAGETVLQATADALTLFSELFGP
                     YPRRTLSVVEADFLDGMEYDGLYFLSKGFYNLYQGTPGEYLVAISAHETAHMWWYALV
                     GNDQAMEPWLDEALCTYSELLFYERYHPEAIGWWWAYRINYYEPRGWVNGSIYNSAGY
                     RAYREAVYLNGAVFLDRLRSEIGPDVFMRFLNAYATQYAYQIATTGNFFSLLKEFSNK
                     DLSDLQSQFFQ"
BASE COUNT          671 a          965 c          777 g          725 t
ORIGIN      
        1 cagctcctcg accgttttgc cctttcggta gagattcacg ggattcgcga tgcccgtgat
       61 cgggtcctga tcatggaacg taacctggcc ttcgaaggcg atcctgagtc tttccagcag
      121 gattggctgc cgcgtgagca agagctttcc cagcaaattg agcatgcccg ccaggtactc
      181 gatcaggtca cccataccag ccgcgatctg ctggcgatcg ctgctttgac ttcttcgttg
      241 aatgtggacg ggcaccgttc cgatctggtg atcctcaaga ctgcccgcgc ccatgctgct
      301 ttcgaaaacc gccgcaccat cgttgaccgc gatattgccc tcgccgccga gttagctttg
      361 cctcatcgca tcaaacgcgg tcctttccat caggccgaga tcaccatgga gcagcttcag
      421 gagcgcattg agcagcttga aggcgctatc gatcactccc aggaaacaga aactcagcct
      481 gtgcaggaac cttccagcga aaaaaaaaag gtctgaatcg ggatatcaag gtcgaggaat
      541 ctgacgaaag ccagcagacg gcttccgaac catcccccca gaacgtcttt gacaccggaa
      601 ccgctaactg gtgggatggc ggcgaaaaga tcaaagtcgg cactccattt gtgccccgcc
      661 gtttagccaa ccccctggac cggatcaccc gccgccacgg tgggcgccgc tcccagaccc
      721 acaccgatcg caaacgcggt cactatatcc aggctcgccc ggcgatgggt aaaaccaacg
      781 acctggcttt tgatgccact ttgcgcgccg ctgcccctta tcaacgcgaa cgcctcgagc
      841 agcgcaagca ggtggcattt gctgtccaga agagcgactt tcagcgtaag gtccgggtga
      901 ccaaagcggc caacctggta ttgtttgtgg tcgatgcttc ctggtctatg gctgtggctg
      961 agcgcatgca ggctaccaaa ggcgccattc tttctttgct gaccgatgca taccaacgcc
     1021 gcgaccgggt tggtctggtc gtctttcaga aagaccgcgc caccctgctc ctctccccca
     1081 ccaattccat ccagcttgcc cagcgctccc tggcagatat ccccgtaggc ggcaagaccc
     1141 cgctgtcggc tggcctgtta ttggcccacc aggtcatcca gcgcgagcgc atcatgcacc
     1201 ccgatgtcat gccgctcatc atcctcctga cggatggcgc cgggaatgtc tcattaagca
     1261 acctgccccc tcaggaggaa tctcaccgcc ttgccgacca gatctccgat gacgagattc
     1321 gctgtgtggt gattaatatg gagcatgctg cttttgatca gggactggcc caggccctgg
     1381 cagaccacct gaaatccccc tgctactctc tgaacgagct caaagcggaa acgctctacc
     1441 gggcggtgcg ggacgaaatg ctgggggcat aagcatcgca tatgcccgcc cttttgtgga
     1501 gcccattcca ccgggacaac cggccggtaa ttcagactgt ggtgctgctg gcattccttg
     1561 tcgctgcatg cacagcaaat tcccggctcc cggatgcttc tcctccgcat acagtcaccc
     1621 ccgagatcct acctgcagag actaacaaca ccccgaccag tccatccggc atccagccca
     1681 cccctgccgg cgcagcagca actgtcgatt caatttccca gcctacaccc accacctcag
     1741 cgccagtccc gctcccgcat tacaccctca ccgccacctt aaactacgca gctcattttc
     1801 tgcaggtcga tgagcggatc gattacacca attcttctgc cgatacgctg actgagctgg
     1861 ttctgatggt ggatgccgtc tatttccccg ggacattcac cctcaagagc ctcacctggg
     1921 tcgactctga agcaaatcct gaagttacct gggacaatgc catcctgcac ctgcagcttc
     1981 cccaaccttt acctcccggc gagcaagtaa gcctcagctt aagctacacc ctgaatcttc
     2041 cttcgccggt tccttccgaa caggtccgtc ccatcccctt cggctacacc gctcggcaga
     2101 ccaatctggt cgattggtac ccatttatag ccccttacat ttctggcgaa gggtggatcg
     2161 ccaaccaggc cggatttttt ggcgagcacc tcgcctatgc cgtttcagac ttcgagatct
     2221 cgatccgcct ggaagatgcc ctcaccggtt tgattgttgc cgccagctcc ccggctgagg
     2281 aggtggacgg ctggctccat taccagatgg aaaaagctcg cagcttcgcc tggtcggtca
     2341 gccacgatta tatcgtcgcc gccacccagg tcggagagac caccgtgacc agctatactt
     2401 ttccggtaca tgccgccgct ggcgaaacag tgctccaggc aaccgccgat gccttgaccc
     2461 tgttctccga gctgtttggt ccgtatccgc gccgaacatt aagcgtggtt gaagccgatt
     2521 ttttggatgg catggaatac gatggccttt attttcttag caaaggtttt tataatctat
     2581 atcagggaac acctggcgaa tatctggttg cgatttctgc ccatgaaacc gctcatatgt
     2641 ggtggtacgc tctggtaggc aatgatcagg ctatggaacc atggttagac gaggcgctct
     2701 gcacctacag cgagctctta ttctatgagc gctaccaccc tgaggcgatt ggctggtggt
     2761 gggcttaccg gatcaattat tatgagcccc gcggctgggt taacgggagc atatataact
     2821 ccgccggcta ccgtgcatat cgagaagctg tttatctgaa tggcgctgta tttttagata
     2881 ggttgcgtag cgaaattggg ccggatgttt ttatgcgctt tttaaatgcg tatgccaccc
     2941 aatacgctta ccagattgcc acaaccggta atttcttttc gctgcttaaa gaatttagca
     3001 ataaagacct ttcagattta cagagccagt ttttccagta gctcgcacat atataatccc
     3061 catatattaa ttaaataact atatagtaat cttagtaggg cttgtggaaa tgtggataac
     3121 tcaactgtgg gtgatcct
//
LOCUS       DATMXK010000346        10705 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_137058, whole genome shotgun sequence.
ACCESSION   DATMXK010000346 DATMXK010000000
VERSION     DATMXK010000346.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10705)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10705)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10705
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_137058"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1201
                     /locus_tag="VN363_06375"
     CDS             <1..1201
                     /locus_tag="VN363_06375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560272.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ84173.1"
                     /translation="LDTDRLTFANLQDSTQDPVITKLVEVGRRQGHITSEDLLESIPE
                     AEHDEELLEDAFRALIDAGIDYFDETDPDNPSSINSAESGPPNLYGEEDFLANIDASD
                     AIGLYLKEATRVPLLNVSEELELAQRIERGKMAREEMVKGNTTVRRQRELRRLVEDSW
                     QAREHLIVANSRLVVSVAKKYMGRGVPFLDLIQEGNIGLMRATKKFDYHRGFKFSTYA
                     TWWIRQAVTRAIADQSRTIRVPVHMGDRISRMLRTQHQLKQRFGREPTNDELAEAMEL
                     PVPKVEQMIKLAQRPLSLQMPIGDEEDNMLGDFIEDVDTPPPDDAATTNLLREYLASL
                     LDQLPPREARILQLRYGLLDGKTLTLNEVGHKMGVTRERVRQIEAQALRRLRASGIQR
                     ELRAFID"
     gene            complement(1254..2453)
                     /locus_tag="VN363_06380"
     CDS             complement(1254..2453)
                     /locus_tag="VN363_06380"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024968.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ84174.1"
                     /translation="MAAERLRILMVADGRSPIALNWIAGLVDAGLEVHLQSTFPARPE
                     LPLASCEVLPVAFGAVSGSASRGEAGSGLRRVIPVGVRTALRQWIVPRSLPKAAGSLQ
                     AAIQMLQPDLIHAMRIPFEGMLSALAVEGLVSAPPLLVSIWGNDFTLHAPATRRMAEL
                     TRLTLKHAAALHADCQRDLRLANEWGFREGQPAVVLPGGGGVQPEIFFPEKASVQSFN
                     EPLTVIQPRGFRAYVQNEAFFRAIPLILHGSPGTRFIFPGMANEAQAQEWVRQFNLSQ
                     SVVLLPFQTRQEMAELFRRAAVIVSPTTHDGTPNTLLEALACGCFPVAGDLESLREWI
                     TPGVNGLLCDPTNPQALADTVVQALASPELRGQAQPINLKLIAERASFPVVMEKAQVF
                     YRQLVKK"
     gene            complement(2443..3612)
                     /locus_tag="VN363_06385"
     CDS             complement(2443..3612)
                     /locus_tag="VN363_06385"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ84175.1"
                     /translation="MESLSHRPMNTHQSTFPGRMAIQQRVLPVYRAPLMDTLAVNCGG
                     GLSVFSGDPLPGEGITAAGDLQVAKRVFARNRHFSNPGSALYQCYQDGLVDWLESWQP
                     DVLIVEANPRYPNTRQGIDWMHTHNRPVLGWGLGAPPIHGPLAAWRRVLRQRLLRSLD
                     GVIAYSQRGAQEYLQAGIAADRIFVALNAAAHRPIQSPPEKPLHFSTQPVVLYVGRLQ
                     ARKRLDLLIQACASLPAALQPHLLIVGDGPAGTDLKTLAGQIYPGTEFTGSLFGEALE
                     ACFQKADVFVLPGTGGLAVQQAMAWGLPVIVAEGDGTQDDLVRSENGWRIIPGSQGDL
                     TSKLQAALSDPGRLRQMGLASYRIVDQEVNLETMAQVFIRAARQVTGMEHGADGC"
     gene            complement(3585..4436)
                     /locus_tag="VN363_06390"
     CDS             complement(3585..4436)
                     /locus_tag="VN363_06390"
                     /inference="COORDINATES: protein motif:HMM:NF016908.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FkbM family methyltransferase"
                     /protein_id="HWQ84176.1"
                     /translation="MSNLLLKLAAQAARSLPESWIQAIYRSPRLANMIRGELNRASPQ
                     GLVPVQIAAGSLQGDTLLLDMQTEKDYWLGVYEPNLQQAIKDLVQPGWVVYDVGANIG
                     YISLIFARAVSRSGHVYAFEALPDNFARVQENIRLNSAQDTITPVHAAVIEADKPVNF
                     LVGPSNGMGKVAGSAGRQEIVYEHSLTVPGISLDEYVFGQGHPAPNVVKMDIEGGEVL
                     ALPGMQRILQEAHPLLLLELHGPESASLARRILTEAGYHLHSMAAGYPEVLAEEALDW
                     KAYLIAR"
     gene            complement(4465..5553)
                     /locus_tag="VN363_06395"
     CDS             complement(4465..5553)
                     /locus_tag="VN363_06395"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ84177.1"
                     /translation="MSQQPLKICLAPRLQGVGGMVSFQARLAGALEARGIAVGFDPAD
                     PAYSAALIIGGTRQLGSFVRARRRGARIVQRLDGMNWIHRRRRTGLRHFLRAEYGNLL
                     LELIRTRIAQHIVYQSHFSQTWWERVHGRAPGSCSVVYNGVDLAIYSPDGPASQNAEP
                     KWDFPFRRNPHRWRILMVEGSLMGGYEQGLESGLLLASGLAAKMPDQPVELVVAGRVV
                     PRVRAAADARAAEMARSHPFSLIWAGLVDRIHIPELDRSAHILFSSDINAACPNSVVE
                     ALACGCPVVAYDTGALPELVTGDSGRLAAYGGDPWKLDPPDQAALVQAALPVLGEQDR
                     FRMAARQRAESELSLDKMVAGYLAALLG"
     gene            complement(5550..6020)
                     /locus_tag="VN363_06400"
     CDS             complement(5550..6020)
                     /locus_tag="VN363_06400"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84178.1"
                     /translation="MDGYTRLVYARQNPVFGRLAYYLLKLLGIEIPRSVPVGEGFILE
                     HGGFGVVIHSRAVIGARVKIYPGVTVGRADIYLPMQQSEFEGVMIEDDAILSPGSRIL
                     GKQGILHVGRGTVVGANAVLLESTGEWEIWAGIPARQVGMRPHPELNLPGDSQR"
     gene            complement(6028..7200)
                     /gene="wecB"
                     /locus_tag="VN363_06405"
     CDS             complement(6028..7200)
                     /gene="wecB"
                     /locus_tag="VN363_06405"
                     /EC_number="5.1.3.14"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016634935.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008761 - UDP-N-acetylglucosamine
                     2-epimerase activity [Evidence IEA];
                     GO_process: GO:0009103 - lipopolysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-N-acetylglucosamine 2-epimerase
                     (non-hydrolyzing)"
                     /protein_id="HWQ84179.1"
                     /translation="MRALRVLSIFGTRPEAIKMAPVVRALEQTPGIESLVCVTAQHRQ
                     MLDQVLNLFHIIPDYDLNLMRPDQSLALLTAAVFEQLDPVLAETQPHWVLVQGDTTTV
                     MAATLAAYYRHIRVGHVEAGLRTGNKWQPFPEEINRRVAGVTADLHFAPTQNARQNLL
                     HEGVGDEMITVTGNTVIDALQAVARLPVNDGFLKYLADLGIPEPFRRADPRSNQSSGP
                     RLVLVTAHRRENFGQPLENICAALRCLAETYGPEIHLIYPVHLNPNVQEPVQRLLGDV
                     PNITLLPPLDYFPMVHLLKQTTLLLTDSGGLQEEAPGLGVPVLVMRQVTERPEGVEAG
                     TVRLVGTDTETILREARRLLDDPEAHRAMAQAVNPYGDGKAAMRIVWSLLDHSTDL"
     gene            complement(7197..8495)
                     /locus_tag="VN363_06410"
     CDS             complement(7197..8495)
                     /locus_tag="VN363_06410"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558670.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003979 - UDP-glucose 6-dehydrogenase
                     activity [Evidence IEA];
                     GO_function: GO:0016616 - oxidoreductase activity, acting
                     on the CH-OH group of donors, NAD or NADP as acceptor
                     [Evidence IEA];
                     GO_function: GO:0051287 - NAD binding [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotide sugar dehydrogenase"
                     /protein_id="HWQ84180.1"
                     /translation="MNFEKICVLGLGYIGLPTASTFATHGLRVIGVDVNPQIVETLRT
                     GGLHIHEPGLRTLVQAALGSGNLTINQEPELADAFIIAVPTPFFDDKRADMRSVIAAA
                     EAIVPYLRPGNLVILESTSPPRTTVDLLVPILEKCGLKAGAIQADQGFLLAYSPERVL
                     PGQILRELIENARVIGGVDRLSAEAGRDLYGLFVRGEIILTNATTAEMVKLMENTYRD
                     VNIAIANEFARLADRFGIDVWEAISIANRHPRVKILKPGPGVGGHCISVDPWFLVEAA
                     PDLTPLIQTARQVNDYQPHVVLEMIREQLGSLEGRRIAALGLSYKPDVDDLRESPAIE
                     IVHLLTQAGAQVLAYEPYKLDAQIPGVACVRELDDALAGADLVAILVAHTPLRELQPE
                     ELLRRTPARLLVDAVNGWAGKDWEAAGFHLSRLGVSEAAR"
     gene            8691..9455
                     /gene="lexA"
                     /locus_tag="VN363_06415"
     CDS             8691..9455
                     /gene="lexA"
                     /locus_tag="VN363_06415"
                     /EC_number="3.4.21.88"
                     /inference="COORDINATES: protein motif:HMM:TIGR00498.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006282 - regulation of DNA repair
                     [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcriptional repressor LexA"
                     /protein_id="HWQ84181.1"
                     /translation="MMMARKRKKGLSDRQKKILEVLDRFQVDPGYPPSIREICDATDI
                     SSTSVVNYYLDQLQEMGFIERDQHVSRGIRLLIPYAEAVNSTGGAIESISSGIKQTAR
                     NLRQAMDELLKIPISGRIVASAPVPVPQSDFGYYDAESMVEVARSLLPGREKGSTDLF
                     ALEVSGDSMIDAMVNDGDIVIMKPTKDVRNGEMVAVWLNDRDETTLKYFYLENGHVRL
                     QPANPTMNPIFIEDPSVVEIQGKVVMVIRQVGGLPS"
     gene            complement(9525..>10705)
                     /locus_tag="VN363_06420"
     CDS             complement(9525..>10705)
                     /locus_tag="VN363_06420"
                     /inference="COORDINATES: protein motif:HMM:NF013125.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="lamin tail domain-containing protein"
                     /protein_id="HWQ84182.1"
                     /translation="IPTLTPTPTGLFKLLISEVLPHPAAGGEPGGEWIELYNAGEWTV
                     CLAGLAIGDEEMRGGSEGMYLFASGECISPNGTSLIAVRADVFEGVFGFSPQYEIDDS
                     DPLVPDLIKDSSWSSGNLSLSNSGDEILLLGLDGVLLDSLSWGNSEWAFSPAIPASAQ
                     GVSLERYPPDQDTDRAADWRHQPAPDPGEAPEQPSATPSPVGTLTPEPEITPLPTGDG
                     SALVVNEIHADPDTWLGDANGDGQVDPNEDEFVEIVNAGTGPVNLSDWRLEVGFNLGV
                     HRFPADTWLEPGSAIVVFGGGTPQGAFGGSLIQIASTGSLNLMNTADIIALKRPDGTL
                     VLTLSYGLEAGDQQSITRSPDITGGEPLVRHSQAAGSGGALYSPGTRLDGSLFPDSGE
                     "
BASE COUNT         2387 a         3255 c         3019 g         2044 t
ORIGIN      
        1 ccttgacacc gatcgactga cgtttgcgaa ccttcaagat tcaactcaag atccggtcat
       61 caccaagctg gtagaggtag gacgccgaca agggcatatc accagcgagg acctactcga
      121 aagtatccct gaagcagaac acgacgaaga gctcctggaa gatgccttcc gcgccttgat
      181 tgacgccggg attgattatt tcgatgagac cgatcccgac aacccatcct cgattaacag
      241 tgctgaaagt ggaccgccca acttatatgg cgaagaggat tttctggcga atatcgatgc
      301 cagcgatgcc atcgggctgt acctcaaaga agccacccgg gttccactcc tcaatgtgag
      361 tgaagagctg gagctagccc agcgcatcga gcgcggcaag atggcgcgcg aagaaatggt
      421 taagggcaat accaccgttc gccgccagcg tgagctgcgc cggttggttg aagacagctg
      481 gcaggctcgc gagcatttga ttgtcgccaa ctcgcggctg gtggtcagcg tcgccaagaa
      541 atacatgggg cgcggggtgc ctttcctcga tttgatccag gaaggcaata taggtctgat
      601 gcgcgccacc aagaagttcg actaccaccg cggcttcaag ttcagtacct acgcaacctg
      661 gtggatccgc caggctgtaa cccgcgccat cgccgatcag agccggacca tccgcgtccc
      721 ggtccacatg ggcgaccgga tcagccgcat gctgcgcacc cagcaccagc tcaagcagcg
      781 cttcgggcgt gagcctacca acgatgagct cgctgaagcg atggaactgc ccgtcccaaa
      841 ggtggagcag atgatcaagc tcgcccagcg tcccctgtcg ctccaaatgc cgattgggga
      901 tgaggaagac aatatgctgg gcgactttat cgaggatgtc gataccccac cgcccgatga
      961 cgcggcaaca accaacctgc tgcgtgagta tctggcgtct ttgctggacc agctgccgcc
     1021 ccgcgaagcg cgcatcctgc agttgcgcta cggcttgctg gatggcaaaa ccctgactct
     1081 gaatgaagtt ggacacaaaa tgggggtgac ccgcgagcgg gtgcgccaga tcgaagccca
     1141 ggcgctgcgc cggctgcgag ccagcgggat ccagcgcgag ctgcgcgctt ttattgactg
     1201 aacttacttt accagacgca atcacaggtg gaggccgccg gcttccacct gttttatttt
     1261 tttacgagct gccggtagaa aacctgagcc ttttccatga caaccggaaa actggcgcgc
     1321 tcagcaatca gttttaagtt gatcggctgc gcctgcccgc gcagctctgg agaggccagc
     1381 gcttgaacga ctgtatcggc gagtgcctgc ggattggtag gatcgcacag caacccgttg
     1441 acccctggcg tgatccactc gcgcagcgat tcgagatccc cggcaaccgg gaaacaaccg
     1501 cacgccagag cttccagaag ggtgttgggc gtgccatcgt gggtcgtcgg tgagacaatt
     1561 accgccgccc ggcggaacag ctcagccatt tcctggcgag tctggaacgg cagcagtaca
     1621 acggattgcg ataggttgaa ttggcgcacc cattcctgag cttgagcttc attggccatc
     1681 ccggggaaga tgaagcgcgt gcctggagag ccatgtagaa taagcgggat agccctgaaa
     1741 aatgcttcgt tctggacata agcccggaag ccgcgcggct ggattacagt cagcggctcg
     1801 ttgaatgact gcaccgaagc tttctcgggg aagaatatct ccggctggac gccaccaccg
     1861 cccggtaaaa caactgccgg ctggccttcc cgaaaacccc actcgttcgc caggcgtaga
     1921 tcccgctggc agtccgcatg cagggctgca gcatgcttta atgtcaggcg ggttaattca
     1981 gccatgcggc gtgtggcagg cgcatgcagg gtaaaatcat tgccccagat cgagaccagc
     2041 agaggcggcg cgctgaccaa tccctcaact gccagggcgg acaacatccc ttcaaatggg
     2101 atgcgcatgg cgtggatcaa atccggctga agcatctgga tcgcggcctg caggctgcct
     2161 gccgctttgg ggagcgagcg cggtacaatc cactggcgca gtgcggtgcg cacgcctacc
     2221 gggatcaccc ggcgcaagcc agagcccgcc tcaccccgac tggctgatcc cgatacagct
     2281 ccaaaagcga ccggtagaac ttcgcaggag gccagcggta actcaggccg ggcaggaaaa
     2341 gtagactgca gatggacttc aagcccggca tccaccagcc cggcaatcca attcagagcg
     2401 atcggtgagc gcccatcggc aaccattaag atccgcagcc gttcagcagc catcagcccc
     2461 atgctccatg ccggttacct ggcgagcggc acgtatgaac acctgggcca tggtctccag
     2521 attgacttcc tggtcgacaa tccggtagga ggccaatccc atctggcgca ggcgacctgg
     2581 atcgctcaag gcggcctgca gcttgctggt gagatcgccc tggctcccgg ggatgatccg
     2641 ccagccattt tcggagcgga ccagatcgtc ctgagtgcca tctccttcgg ccacaatgac
     2701 cggcaagccc caggccattg cctgctggac cgccaatcct ccggtccccg ggaggacaaa
     2761 cacatccgcc ttttgaaaac aggcttccag agcctctcca aaaagagacc cggtaaattc
     2821 ggtgcccgga taaatttgtc cggcgagcgt tttcaaatcc gtacccgccg ggccatcgcc
     2881 gacgatcaac aaatgcggct gcagggctgc aggcagactg gcgcaggcct ggattaataa
     2941 atccagccgc ttgcgagcct gcaagcgacc tacatacaat acgaccggtt gagtactgaa
     3001 atgcaagggc ttttctgggg gcgattggat aggccgatgg gcagccgcat tcagggcgac
     3061 aaaaatgcgg tctgcagcta tgcctgcctg caggtattcc tgcgccccac gctggctgta
     3121 tgcaatcact ccatccaggc tgcgcagcaa gcgttggcgg aggacccgcc gccaggctgc
     3181 gagcggaccg tggatgggcg gggcacctaa cccccagccc agcacagggc ggttgtgagt
     3241 atgcatccag tcgatcccct ggcgcgtgtt gggatagcgc gggttagcct ctacgatcag
     3301 cacatccggc tgccagctct ccagccaatc aaccaggcca tcctggtagc attggtagag
     3361 cgctgagcca gggttcgaaa aatggcggtt gcgagcaaaa acgcgctttg ccacctgcag
     3421 gtcgccggcc gcagtgatgc cttcacccgg cagcggatca ccggaaaaga cactcagacc
     3481 tcccccacaa ttcaccgcca gcgtatccat cagcggggcg cgatatacgg gcagcacgcg
     3541 ctgctgaatt gccatccggc cagggaaagt ggattgatgg gtgttcatcg ggcgatgaga
     3601 taggctttcc aatccaatgc ttcttcggca agcacttctg gataaccagc ggccatgctg
     3661 tgaagatggt agccggcctc agtcaggatc ctgcgcgcca ggctggcgga ttcgggtccg
     3721 tgcagttcca gcagcagtaa gggatgggct tcctgcagaa tgcgctgcat ccccggcagg
     3781 gcgagaacct caccgccttc gatatccatc ttcaccacgt ttggagccgg gtgaccctgc
     3841 ccaaacacat actcatccag cgagatcccc ggcaccgtca gggagtgctc ataaacgatt
     3901 tcctggcgtc ctgccgagcc tgcgaccttt cccatcccat tggatgggcc taccaggaag
     3961 ttgacaggtt tatcggcctc aatgacggca gcatggaccg gggtaatcgt atcctgagct
     4021 gaattcaaac ggatattttc ctgcacccgg gcaaagttgt ccggcaaagc ttcgaaagcg
     4081 taaacgtggc cggagcgaga aacagccctg gcgaagatca ggctgatata accgatattg
     4141 gcgcccacat cataaaccac ccagccgggc tggaccaggt ctttgatcgc ctgctgcaaa
     4201 tttggctcgt aaacacccag ccagtaatct ttttcagttt gcatatccag cagcagggta
     4261 tctccctgca ggctgccggc cgcaatctgg acgggcacca gcccttgcgg agaagcgcgg
     4321 ttcagctcgc cgcggatcat attcgccagg cgcgggctgc ggtatatcgc ctggatccag
     4381 ctttcgggca ggctgcgggc tgcctgggca gcgagtttca acaacaaatt actcatgcga
     4441 gcacgtccat tcgcgtcccg gcgctcagcc cagcagcgcc gctaaataac ctgccaccat
     4501 tttatccaga gagagctcac tttcagcgcg ctggcgagcc gccatccgaa agcgatcctg
     4561 ttctcccagc actggcaggg cagcctggac aagggcagcc tggtccgggg gatcgagttt
     4621 ccaggggtcg ccgccataag ctgccagccg cccgctgtcc cccgtgacca gctcaggcag
     4681 agcaccggta tcataggcaa ccaccgggca gccgcatgcc agggcttcga ccaccgaatt
     4741 cgggcaggca gcgttgatat cgctggagaa tagaatatgg gcagaacgat ccagctcggg
     4801 aatatgaatg cgatccacca gacctgccca gatgagcgaa aacggatggc tgcgagccat
     4861 ctcagctgcc cgggcatccg ccgctgcccg gactcgcggc acaacacgcc cggcgaccac
     4921 cagctcaacc ggttgatcag gcattttagc tgccaatccg gaggccagga ggagaccact
     4981 ttccagaccc tgctcgtagc cgcccatcag gctgccttcg accatcagga tccgccagcg
     5041 atgcgggttt cgccggaaag gaaaatccca tttaggctcg gcattctggc tcgccggtcc
     5101 atccggggaa taaattgcta aatctacccc attgtaaacc acactgcagc tacccggcgc
     5161 ccggccgtga acccgctccc accaggtttg cgaaaagtgg ctctggtaaa caatatgctg
     5221 ggcaatgcga gtgcggatta attccagcag caaattgccg tattcggcgc gcaggaagtg
     5281 gcgcagtccc gtccggcgcc ggcgatggat ccagttcatc ccatccaggc gctggacaat
     5341 gcgcgccccg cgccgccggg cacggacgaa actacctaac tggcgtgtac cgccaataat
     5401 caaagccgcc gaataagccg ggtcggccgg atcaaagccc actgcgattc cgcgcgcctc
     5461 cagcgcacca gccaggcgag cctgaaaaga gaccatgccc cccacaccct gcaagcgcgg
     5521 cgccaggcaa attttgagtg gctgctggct caccgctggc tgtccccggg taggttaagc
     5581 tccggatgtg ggcgcattcc cacctggcgg gcagggatgc cggcccagat ctcccattca
     5641 ccggtggatt cgaggagcac tgcgttggcg ccgactaccg tgccccgccc gacgtgtaga
     5701 atgccctgct tgcccagaat acgtgaaccc ggagagagga tcgcatcgtc ctcgatcatc
     5761 acgccttcaa attcggattg ctgcatgggc aggtatatat ctgcccggcc aaccgtaacc
     5821 cccggataaa tcttcacgcg ggctccaatg accgcccgcg agtgaatcac caccccaaaa
     5881 ccgccgtgtt ccaggatgaa gccttcccca accggcacgc tgcgcgggat ttcgatgccc
     5941 aacaatttta aaagatagta agccaggcgg ccgaacacag gattttggcg ggcataaacc
     6001 agccgggtgt aaccatccat aaggtgctca gaggtccgtg gaatgatcca acaatgacca
     6061 gactatgcgc atggcggctt tcccatcccc atagggattc accgcctggg ccattgcccg
     6121 gtgagcttcc gggtcatcca ataaacgccg cgcttcgcgc aggatcgtct cagtatcggt
     6181 gcccaccagg cgcaccgtgc cggcttccac accctcaggg cgttcggtaa cctggcgcat
     6241 caccagcacc ggcacgccca ggccgggagc ctcttcctgg agaccgcctg aatcggtcag
     6301 cagcagggtc gtttgcttga gcaggtgcac catcgggaaa taatccagcg gtggaagcag
     6361 ggtgatgttc ggaacgtccc ccaggaggcg ctggactggc tcctgcacat tcggattgag
     6421 gtggacagga taaatcagat ggatctccgg accgtaggtc tcagccagac agcgcaaagc
     6481 tgcgcagata ttttccaacg gctggccaaa attttcgcgg cggtgagcag tgaccaacac
     6541 taggcgcggc cccgaactct ggtttgaacg tggatctgcc cggcggaatg gctcggggat
     6601 acccaggtct gccagatatt tcaggaagcc atcattgaca ggcaagcgcg ccactgcctg
     6661 cagcgcgtcg atcaccgtat tgcccgtcac tgtgatcatt tcgtcgccga caccttcatg
     6721 aagcaggttc tggcgggcat tctgggtcgg ggcgaaatgc aggtcggcgg tcacaccagc
     6781 tacccggcgg ttgatttcct ccgggaatgg ctgccattta ttgccggtgc gcaagccagc
     6841 ctctacatgc cctacgcgga tgtgccggta ataggctgcc agggttgccg ccataaccgt
     6901 ggtggtatcg ccctgcacca gcacccagtg gggttgagtc tctgccagga ccgggtcaag
     6961 ctgctcaaag accgctgcgg tcagcagagc caggctctgg tccgggcgca tcaggttcag
     7021 atcataatct ggaatgatat gaaacaggtt gagcacctga tccagcatct ggcggtgctg
     7081 ggcggtcaca cataccagcg attcgatccc cggagtctgt tccagggcgc gcaccaccgg
     7141 ggccattttg atcgcttccg gtcgcgtgcc gaatatcgac aggacccgca gcgctctcac
     7201 cgggcagcct cgctaacccc cagccgtgag agatggaagc cggctgcttc ccaatctttg
     7261 ccagcccagc cgttgacggc atccaccaga aggcgggcag gcgtgcgtcg cagcagctct
     7321 tcaggctgga gctcacgcaa aggtgtgtgc gccaccaata tagcaaccag gtctgcgcct
     7381 gccagggcgt cgtccagctc gcgtacgcac gccacgccag ggatttgagc atccagcttg
     7441 tagggctcat aagccagcac ctgagcgcca gcctgggtga gtaaatgaac aatctcgatc
     7501 gccgggctct cccttagatc atctacatct ggtttataag aaagccccag ggcagcaatt
     7561 cgccgccctt ccaggctgcc gagctgctcc cggatcattt cgagcacaac gtggggctgg
     7621 tagtcgttaa cctggcgcgc ggtctggatc aatggagtca ggtcaggggc agcttcgacc
     7681 agaaaccagg gatcgacgct gatgcagtgc ccgcccacgc cggggccagg tttcaggatc
     7741 ttcacccgcg ggtggcggtt ggcaatgctg atcgcctccc aaacatcaat tccaaaacgg
     7801 tctgccaacc gggcaaactc attggcaatg gcgatgttca cgtcgcgata tgtattttcc
     7861 atcaatttga ccatctcagc ggtggtcgcg ttggtgagga tgatttcgcc gcgtacaaac
     7921 agaccataca aatcccgccc ggcttcagcg gaaaggcgat cgactccacc gatcacgcgc
     7981 gcattttcga tcaactcacg tagaatctgc ccggggagca cacgctcggg cgaatatgcc
     8041 agcaagaaac cctgatccgc ctggatggca ccggctttta acccacactt ctctaatatg
     8101 ggtaccagca gatcgacagt ggttcggggg ggtgaggttg attccaagat caccaggttg
     8161 ccaggacgca gataaggaac gatcgcttct gcggctgcaa tcactgagcg catgtcggcg
     8221 cgtttatcat caaaaaaagg ggtcgggacg gcaataataa aagcatccgc cagctcaggt
     8281 tcctggttaa tggtcaggtt acccgatccc agagctgcct gaacgagggt gcgcagacca
     8341 ggctcatgaa tgtgcaatcc gccagtccgc aaggtttcaa caatctgcgg attgacatca
     8401 accccaatga cccgcaaacc atgcgtcgca aatgtactgg ccgtaggcaa accaatatac
     8461 cccagcccaa gcacacagat cttttcaaaa ttcacaggag agctccttcg ttgaatttcc
     8521 caggcggaaa tagctatcag ccggtctccg cctccatgga ttaaaccaca ttcagccgcg
     8581 ctcggatagg attgcccata aagtcataaa tgcctcttga cacagaacgc atgttcgtgt
     8641 tatcataacg ataactgaac ggttgttcta cccgaatagg agagtggaca atgatgatgg
     8701 cccgcaaacg caaaaaaggc ctcagtgatc ggcagaagaa aatcctggaa gtgctcgacc
     8761 gtttccaggt tgaccccggg tatccaccat ccatccgtga aatatgtgat gcaacggata
     8821 tctcttcgac ttcagtggtg aattattatc tggatcaatt gcaagagatg ggtttcatcg
     8881 agcgcgacca gcacgtatcg cggggtatcc gtttactgat tccttacgcg gaagctgtca
     8941 attcaaccgg tggcgccatc gaatcgatct ccagcggcat taaacaaaca gcccgcaatc
     9001 tgcgccaggc aatggacgag ctgctcaaga tcccgatcag cgggcggatc gtcgcttcgg
     9061 cgccggtccc ggtccctcag tcggacttcg gctattacga tgctgaaagc atggtcgaag
     9121 tagcccgcag cctgcttccg ggccgtgaaa aaggctctac cgatctattc gctctcgaag
     9181 taagcgggga ttcaatgatc gacgccatgg ttaatgatgg cgatatcgtg atcatgaaac
     9241 ctaccaaaga tgtacgcaat ggtgaaatgg ttgctgtctg gctgaacgac cgggatgaaa
     9301 ctaccctcaa gtacttctac cttgaaaacg gccatgtacg gctgcagccg gctaacccca
     9361 caatgaaccc aatctttatt gaagatccca gtgtggttga aatccaggga aaagttgtca
     9421 tggtgatccg ccaggttggc gggctgccat cctgaagtga tcagctcgaa cgatgaaaaa
     9481 ggctgcctga taatgtgggc agtctttttt tcttcgccat acggctactc tcctgaatcg
     9541 gggaataaac taccatccag gcgcgtgcca ggcgaataca acgctccacc cgagccggct
     9601 gcctgactgt gcctcaccaa aggctcaccg cccgtaatgt ccggtgatcg agtgatcgac
     9661 tgttgatcgc ctgcttccaa accataactt aaggtaagca ccagtgtacc atctgggcgt
     9721 ttcagagcaa tgatgtcggc agtgttcatc aggttcagac tgccagtcga agcaatctgg
     9781 atcaggctgc cgccaaaggc tccttgaggt gttccgccgc caaagacaac aattgctgat
     9841 cccggctcta accaggtatc cgccggaaag cgatgcacgc ccaggttgaa accgacttcg
     9901 agccgccaat cgcttaaatt aactggaccg gtcccggcat tgacaatctc tacaaattca
     9961 tcctcgttcg gatcgacctg cccatcgcca ttagcatccc ccagccaggt atccggatcg
    10021 gcatggatct catttaccac cagcgcactg ccatccccgg taggcagtgg agtaatttcc
    10081 ggctctgggg ttaatgtccc gacaggcgaa ggggtggcgc tgggctgctc cggagcttcg
    10141 ccgggatctg gtgctggctg gtgccgccag tcggcggcgc ggtcagtatc ctggtccggg
    10201 ggatatcgtt ccagcgagac cccttgcgca gaggcaggta tagcaggcga aaacgcccac
    10261 tcggagtttc cccacgacag actgtccagc agtaccccat ccagcccaag caagagaatt
    10321 tcatccccac tattgctcaa gctcaggttc ccactcgacc aggagctatc tttaatgaga
    10381 tcgggtacca gcgggtcact gtcatcgatc tcatattggg gagagaatcc aaacacaccc
    10441 tcgaatacat ccgcccggac agcgatcagg ctggttccat tgggtgaaat acactcgccg
    10501 ctcgcgaaga gatacatccc ctcgctgcca ccccgcatct cctcatcgcc gatggccaga
    10561 ccagccaggc agacggtcca ttccccggca ttataaagct cgatccactc gcctcccggc
    10621 tcaccgccgg cagcgggatg tggcagcact tcgctgatca acagcttaaa cagtccggtt
    10681 ggagtcgggg ttagcgtggg aatgg
//
LOCUS       DATMXK010000347         2313 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_120017, whole genome shotgun sequence.
ACCESSION   DATMXK010000347 DATMXK010000000
VERSION     DATMXK010000347.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2313)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2313)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2313
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120017"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..468
                     /locus_tag="VN363_06425"
     CDS             <1..468
                     /locus_tag="VN363_06425"
                     /EC_number="3.1.1.31"
                     /inference="COORDINATES: protein motif:HMM:NF013356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphogluconolactonase"
                     /protein_id="HWQ84183.1"
                     /translation="AFYPVPGRSSGDSDTLQACRDYEALLRAHPADLCALGIGENGHL
                     AFNDPPFADFNDPVWVKMIKLDEVSRQQQAGEGHFPDIDSVPTHAITLTIPALLAARK
                     VLAIVPEARKAAAVKRSLTGPVDPGCPASILRQTPHAHLFLDRDSASQAFPSV"
     gene            477..1181
                     /locus_tag="VN363_06430"
     CDS             477..1181
                     /locus_tag="VN363_06430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84184.1"
                     /translation="MPADLAPQSPSLFVLGDSISIHYGPFLERILAGCFVCDRKGSEL
                     LRDEPPDYRQGDILQRLAVLENSSDAINGGDSACVLAYLQVRCMLPPPGWTVLLLNCG
                     LHDLRTDPQVGTHQVDLEAYAVNLSEILRLAALLSRRVVWLRTTPVVDARHQRLNPEY
                     HRFNADVELYNRVADQLMDRAGIPIIDLYGFTHRLSSSSESLDALYSDHVHFIEPVQQ
                     LQAAYIAGWLDACSFS"
     gene            1162..2250
                     /locus_tag="VN363_06435"
     CDS             1162..2250
                     /locus_tag="VN363_06435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84185.1"
                     /translation="MPAHSPDPVSSPARPFLPVELVFNPNWWHQTAGISFDHSFYLDP
                     RARIENDVLMRKVLYRRYGYLGLGEPDPQPRPVIGSLHVAGGFVIPALLGAQIMFAPD
                     AAPQPLPMGLSAAQIDRLEAVDFRQLWPLNELIAQMDSLEAEWGYLVGDLNTDGLLNA
                     AYHLYSADLFADFHNAPRRVQRLLEMIGELIVDVALYVRQRTGSCSIAVNRMVARVDQ
                     RMFLHANCSVPMISPKSYQAVQLPVELQMAQRIQPFGIHHCGDNLQRYISSYRALPLA
                     FVDVGWGSDVAACRQALPDTFFNLRLSPVRMLTCTPQEIARDTENLLRAAGPLEQAGI
                     CCINMDYGTPDDNLFAMYAVVERFRGNS"
BASE COUNT          404 a          740 c          633 g          536 t
ORIGIN      
        1 gcgttttacc ccgtccccgg cagaagttcc ggcgattcgg ataccctcca ggcctgccgg
       61 gattatgagg ctctgctgcg cgctcatccg gccgatttgt gcgctcttgg catcggcgag
      121 aacggccacc tcgccttcaa cgatccccca tttgccgact tcaacgatcc cgtgtgggtc
      181 aagatgatta agctggacga agtctcccgg cagcagcaag ccggcgaagg tcacttcccg
      241 gatatcgatt ctgttcctac ccacgccatc acactgacca taccggcctt gctggctgcc
      301 cgcaaggtcc tggcaattgt ccccgaggcc cgcaaagccg cagccgtcaa gcgctcactc
      361 accggtccgg ttgatccggg ctgccctgcc agtattctgc gccagacacc ccatgcccat
      421 ctgtttctgg atcgcgactc ggcttcccag gcttttccat ctgtatgagc ttgaaattgc
      481 ccgctgatct cgcgccccaa agcccatccc tttttgtcct cggggatagc atttcgatcc
      541 attatgggcc attcctggag cgcatcctgg ccgggtgctt tgtctgtgat cgcaagggta
      601 gcgagcttct gcgggatgag cctcccgatt accggcaggg ggatatcctc cagcgtctag
      661 ccgtgctgga gaacagctcg gatgcgatta atggcggtga cagcgcctgt gtgctggcct
      721 accttcaggt gcggtgcatg cttccgccgc ctggctggac cgttctctta ctcaactgcg
      781 ggctgcacga tctgcgcaca gatccccaag tgggtactca ccaggtcgac ctcgaagcct
      841 acgccgtaaa cctgagcgaa atcctccgcc tggcagcgct tctcagccgc cgggtggtct
      901 ggctgcgcac cacgccggtc gttgatgcgc gtcaccagcg tttaaatccc gaatatcatc
      961 gcttcaacgc ggatgttgag ctttataacc gcgtcgcgga tcagctcatg gaccgggctg
     1021 gaatccccat tatcgacttg tatggcttca cccaccgttt gagttcttcc tccgaatccc
     1081 tggatgcgct gtacagtgac catgtccatt ttattgagcc tgtccagcag ctgcaggccg
     1141 cctatatcgc cggatggtta gatgcctgct cattctcctg accccgtttc gtctcccgca
     1201 cgtcctttcc tgccggttga gctggtcttt aatcccaact ggtggcatca gaccgccggt
     1261 atatcctttg accattcgtt ctatctggat ccccgggcgc gcatagagaa cgatgtcctg
     1321 atgcgtaaag tgctttaccg gcgctatggt taccttggcc tgggcgagcc ggaccctcag
     1381 ccccgcccgg tgattggttc gctgcatgtc gctggaggtt ttgtgatccc ggctctgctt
     1441 ggcgcacaga tcatgttcgc gcccgatgcc gccccgcagc cgctccccat gggcttaagc
     1501 gctgcgcaga tcgatcgcct ggaggcagtc gactttcgcc agctctggcc gctgaatgag
     1561 ttgatcgccc agatggattc cctggaggct gaatggggct atctggttgg agacctgaac
     1621 accgatggcc tcctcaacgc ggcctaccac ctctacagcg ctgacctgtt cgccgatttc
     1681 cataatgccc cccggcgtgt gcagcgctta ctggaaatga ttggcgagct gatcgtggat
     1741 gtggcgctgt atgtacgcca gcgcaccggc agctgctcga ttgccgtaaa ccgcatggtc
     1801 gcccgggttg atcagcgtat gttcctgcat gccaactgct cggtacctat gatctcaccc
     1861 aaaagctacc aggctgtgca gctgccggtc gagctgcaaa tggcgcagcg aatccaaccg
     1921 ttcggcatcc accactgcgg cgacaacttg cagcgttata tcagctctta ccgcgccctg
     1981 cctctggcgt ttgtcgatgt tggttggggc tccgatgtag ccgcctgtcg ccaggcattg
     2041 cccgacacgt tcttcaacct gcgtctcagc ccggttcgca tgctgacctg cacgccccag
     2101 gaaatcgccc gggataccga aaatctgctc cgggctgcgg gtccacttga gcaggccgga
     2161 atctgctgta tcaacatgga ttatggcacc ccggatgata atctgtttgc catgtacgcg
     2221 gtggtcgaac gcttccgggg aaacagctga acaaataagg aaatcaccga tggaatatcg
     2281 tctgtttggc cgcacaggag tccgcgtatc gcc
//
LOCUS       DATMXK010000348         2119 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_42629, whole genome shotgun sequence.
ACCESSION   DATMXK010000348 DATMXK010000000
VERSION     DATMXK010000348.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2119)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2119)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2119
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42629"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1312
                     /locus_tag="VN363_06440"
     CDS             <1..1312
                     /locus_tag="VN363_06440"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWQ84186.1"
                     /translation="VNSAEDDANLSDNDPAGGSGYYASQERIAVPRVLNGLLESGLLR
                     SQPGGRLRMAHPVLLGYLAAGADTQPEQIQSLLNQPDWLGKSLAARYLAMRVDLSDWL
                     EQIAPPPDIDTLQSGLLETARWLREMPERLAMRADLLRRLAAILQNGELALELRARSL
                     AAIAATGSPQMGILFRKLLESPDEGSRRLAALGCGALQDLKAAPILENLLGDPSWQVR
                     RAACLALVATGSQPALEATAASLLHGDDELRKAAAEALANHPEEGYPVLEEASALPDL
                     LVRRAAVAGLGRIRQPLVASKWAMEILQKLQLEDSQWVVQNAAAQALEEMIQTNARSP
                     RPMPALTQTPWLIAYASELGMGVAPGKPAWDLLFKALQSGSLEQRLAALEVLKRHGKE
                     NAILPLYDIYFAGNGEVREAAYQTLWHLRTAGTPLPAPRKFGVA"
     gene            complement(1347..1658)
                     /locus_tag="VN363_06445"
     CDS             complement(1347..1658)
                     /locus_tag="VN363_06445"
                     /inference="COORDINATES: protein motif:HMM:NF014633.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF167 domain-containing protein"
                     /protein_id="HWQ84187.1"
                     /translation="MKSRPYHLHNGQKGSALAIRVTPRASRNEISEILNDGTVKIRLT
                     APPAEGKANQALIEFLASVLNLPPSRIELIAGATGRDKLVSILDLDAETVHQRILQHL
                     A"
     gene            complement(1681..1944)
                     /locus_tag="VN363_06450"
     CDS             complement(1681..1944)
                     /locus_tag="VN363_06450"
                     /inference="COORDINATES: protein motif:HMM:NF014389.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YggT family protein"
                     /protein_id="HWQ84188.1"
                     /translation="MVIFLIRLVDALATILVFAIIAQTLLSYFMAPYHPVRQTLDRII
                     GPMLNPIRRVLPTLGGLDFSPLVLVILIQIVESLLTRILRNFV"
BASE COUNT          456 a          627 c          651 g          385 t
ORIGIN      
        1 ggtaaattca gctgaggatg atgcaaatct ctccgataac gatccagccg gcggaagtgg
       61 atattatgca tcgcaggaac ggatcgccgt gccgcgtgtc ctgaacggct tgttggaaag
      121 cggcctgctg cgctcacaac ccggcggccg gctgcgtatg gcgcacccgg tcctgcttgg
      181 gtacctggca gccggggcgg acacccagcc cgagcagatc cagagcctgc tcaaccagcc
      241 cgactggctg ggaaaaagcc tggcagcgcg ctacctggca atgcgcgttg acttgagcga
      301 ttggctggag cagatcgcgc cgccacctga tatcgatact ttgcagtccg gcttgctgga
      361 aactgcccgc tggctgcggg agatgccgga gcgcctggcg atgcgggccg atctattacg
      421 ccgcctggcg gcgatcttgc agaatggcga gctggcattg gagttacgcg cccgcagcct
      481 ggcagcgatt gctgctaccg gaagcccgca aatgggaatc ctcttccgca agctattaga
      541 aagcccggat gaaggctcac gccggctggc tgcattgggc tgcggtgcac tgcaggacct
      601 caaggcggca ccgatccttg agaacctgct gggagatcca tcctggcagg tgcggcgggc
      661 agcctgcctg gcgctggtcg ccactggcag ccagcctgcc ctggaagcga ccgcagccag
      721 cctcctgcat ggggatgacg agctgcgcaa agcggctgcc gaagccctgg caaatcatcc
      781 cgaggaaggc tatccggtac tcgaagaagc cagcgcactg cccgatctac tggtccgccg
      841 ggcggctgtt gccgggctgg gccggatccg ccagccgctg gtcgccagca agtgggcgat
      901 ggaaatcctg cagaaattac aattagaaga cagccagtgg gttgtccaga atgccgccgc
      961 gcaggcattg gaagagatga tccagaccaa tgcccgcagc ccacggccca tgccggctct
     1021 cacccagact ccctggctga tcgcttatgc cagcgagctg ggcatgggcg tcgcacctgg
     1081 taaacctgcc tgggacctgc tcttcaaagc cctccaatcg ggaagcctcg aacagcgcct
     1141 ggcagccctg gaggtgctca agcgccacgg caaggagaat gccatcctgc cgctgtatga
     1201 tatctatttc gccgggaatg gtgaggtgcg cgaagctgct taccagaccc tctggcactt
     1261 gcgaacagcc ggaacgcccc tgcccgctcc tcgcaaattt ggcgtggcct gagactattc
     1321 ccccagttaa accacaaacg cgttgattaa gctaaatgtt ggagaatgcg ctggtgtacg
     1381 gtctcggcat ccaggtcgag gatcgaaacc agcttgtcgc gaccggttgc accggctatc
     1441 agctcgatcc ggctgggagg gagattcagg acactggcca gaaattcgat cagcgcctgg
     1501 ttggctttgc cctcagcagg cggcgcggtc agccggatct tcaccgtacc gtcattcaag
     1561 atctcggaga tttcattccg gctggcgcgt ggggtaaccc ggatcgccag ggcggagcct
     1621 ttttggccgt tatgcaggtg gtatgggcgt gatttcatca agaattcctt cccccagtcg
     1681 tcagacgaaa ttccgcagga tgcgggttaa gagcgactca acaatctgga tcaggatcac
     1741 caaaactaaa gggctgaaat ccagacctcc cagcgttgga agcacacgcc ggatgggatt
     1801 caacatggga ccaataatcc gatccagggt ctggcggact ggatggtagg gtgccataaa
     1861 atagctcagc agggtctggg cgatgatcgc aaataccagg atcgttgcca gagcgtccac
     1921 aagccggatc agaaaaataa ccatagatgc aaacctcagt gtatgaatga cactgccgga
     1981 agggcgcagg ggcgcagacc cataatggca gtgccgatcc ttaaaattgt ggcgccttcc
     2041 tgaattgcca ccggataatc acccgacatg cccatcgaaa gctcgcggca atcgaagccg
     2101 ggcagcgcgg tacgcagat
//
LOCUS       DATMXK010000349         6704 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_115028, whole genome shotgun sequence.
ACCESSION   DATMXK010000349 DATMXK010000000
VERSION     DATMXK010000349.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6704)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6704)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6704
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115028"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..460
                     /locus_tag="VN363_06455"
     CDS             <1..460
                     /locus_tag="VN363_06455"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84189.1"
                     /translation="SGTVRGVYLAGGFALPIIQQPEDKPAYVSNRTGLVTQFNAPSNY
                     GVTGLLAHNYLAGEEFYKLRTGQEVALIYGDSLIKRYQVARIDTFQKLTPSRLKSDYL
                     ELSTGRRLSTSQMFKRFYRGDEHLTFQTCLEKDGLLNWGLLLVTAVPVDS"
     gene            complement(540..1610)
                     /locus_tag="VN363_06460"
     CDS             complement(540..1610)
                     /locus_tag="VN363_06460"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWQ84190.1"
                     /translation="MRKTHLLIVLGSIVSLLMLAACGSAENPEGTPVFTEPAMDLTMT
                     PEGEFTEEPEATDVPEVTEPPTATEPGVETQPAVTEPATTLPATTSEVLPPTGFVDPS
                     LVSNQLDYDVWNLEDEQIGTAEDMILNLEEKKIDYLIVEAGGFLGIGGKLVAVPYDRL
                     QLADADATSESEPQNVWILDVSREELEGSAEFDREALPLLGEPAVDYDLEFRSHWEAV
                     PAADTTGTPEAAATDAPSEMADGKLTGVVLASQVLDMNFVDANDGELASIEDIVVNPE
                     TGEIRYVVVQIGVDIADLNGRWVLIPVDAINLDATNEALVITVDDALATGIPSFAPNE
                     LPDTTQDDWDADIVDYWNALIR"
     gene            1831..2196
                     /locus_tag="VN363_06465"
     CDS             1831..2196
                     /locus_tag="VN363_06465"
                     /inference="COORDINATES: protein motif:HMM:NF017088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PRC-barrel domain-containing protein"
                     /protein_id="HWQ84191.1"
                     /translation="MKTEYRTPVALSSSTLTGDPVHNLAGETLGKLEEIMIDLESGRV
                     AYVVLSFGGFLGMGDKLFAIPWEAITVDTDKKAIVLNVSKEVLENAPGFDKDHWPDTS
                     EHAWLTDIYAYYGFDPYWR"
     gene            complement(2330..2566)
                     /gene="chaB"
                     /locus_tag="VN363_06470"
     CDS             complement(2330..2566)
                     /gene="chaB"
                     /locus_tag="VN363_06470"
                     /inference="COORDINATES: protein motif:HMM:NF007136.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="putative cation transport regulator ChaB"
                     /protein_id="HWQ84192.1"
                     /translation="MPYKSNSELPDGVQNVLPDHAQDIYREAFNSAWEQYDTPQERRG
                     DSSREETAHKIAWAAVKKNYQKDDSSGKWRRKKS"
     gene            complement(2611..2907)
                     /locus_tag="VN363_06475"
     CDS             complement(2611..2907)
                     /locus_tag="VN363_06475"
                     /inference="COORDINATES: protein motif:HMM:NF024143.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YtxH domain-containing protein"
                     /protein_id="HWQ84193.1"
                     /translation="MKTNFITLVSVFIAGAAAGTAVALLNASRPGKETQAQLREGISE
                     AGMRTKEAISDVQARTMAKMDEVKQRVKEIGDEAKQIPEKLVNTKITIGDLHEE"
     gene            3189..4169
                     /locus_tag="VN363_06480"
     CDS             3189..4169
                     /locus_tag="VN363_06480"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84194.1"
                     /translation="MSDETIDTAVENPENIIQTKNVTKEFNGTAAVQELSFYVPRGKI
                     FGFIGPSGSGKTTTLRMLTGYYHPTSGEVRVLGRSPEHFTRAERENIGYLPQMFSLYP
                     YLTVWENLNFAASIYGLSLARRKRLENILEFVELSGDRDKLASQLSGGMQRRLNLAAT
                     LLHNPDLIFLDEPTAGVDPVLRSKFWEHFRELQAQGRTMFVTTQYVSEAAYCDLVGVI
                     DQGRLVIVDTPALLRYRAFGGDLVDLHTVAPLDYATLQAVRELPFVVSRVSQQSPTSV
                     RLVVKEAKTDIPALVDWARERNMEIEAIEEYLPPFDDVFVKLVKEGVENG"
     gene            4162..5703
                     /locus_tag="VN363_06485"
     CDS             4162..5703
                     /locus_tag="VN363_06485"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84195.1"
                     /translation="MGNPISALWKVFIRISAFLRKEVVIILRQPQLLMTLVLGPFLIL
                     LVFGLGYRNEPPVLRTLFVAQPGSQLEEQIKAYAPTLGPQLSFLGVTGDREAAMESLR
                     RGDLDLVAVAPQDAAASIQAGQQAEFQLYHADIDPTQASYISYFGEIYIAEVNRRVLQ
                     SLTAEGQVQAEDLQQNLQSARAETQSAEQALQAGDEVAARQHLAGLTSNLDQLSLAVG
                     ASLAVLNNVEGTVGSESNQQSLEISRLLNDIQTLSDELGEAQNTTSDPGQWQTRVAEI
                     NSNLNDLDAQLQTFTQLKPEVIVEPFTSTAQSIAEVQPDATNFFAPAVLALLLQHLAV
                     TIAGLSIVHERNFGTLELFRVAPLSAGEILIGKYLSYLFFGAIVAAALTALLHLALGV
                     PMLGNWWNYALVIGVLLFTSLGVGFVISLISRSDSQAVQYTMIALLVSVFFSGFLMSL
                     NMLAEPVRSVSWGIPTTYGIMLLRAVALRGSFPDLVLVGILAAIGVALALLAWLILRR
                     MISNQ"
     gene            5812..6213
                     /locus_tag="VN363_06490"
     CDS             5812..6213
                     /locus_tag="VN363_06490"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84196.1"
                     /translation="MLSEKQSPKFFRPILILALTLAVGLLAACSSTTLPDTGSLGTAV
                     DQPPEAVLQTVERLSQELGVAVEEIEIVEFEQVEWPDACLGFPQEGQACAQVVTPGFR
                     VLLAVGDQQYEFRSDESGTVIVKTTPEAPAP"
     gene            6405..>6704
                     /locus_tag="VN363_06495"
     CDS             6405..>6704
                     /locus_tag="VN363_06495"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Presence of a B(12) (cobalamin)-binding domain
                     implies dependence on cobalamin itself, in one of its
                     several forms, or in some unusual lineages, dependence on
                     a cobalamin-like analog; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology.
                     GO_function: GO:0031419 - cobalamin binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cobalamin-dependent protein"
                     /protein_id="HWQ84197.1"
                     /translation="MSRVALVFPYFRTHAPTEMLFPPLGASSLAAQLRKLNIECHIFD
                     CTFGSFPQLQKDLLAYQPEIVGIYSMVTLSRNTFRIAEMVRKHLPTALLVAGGPLP"
BASE COUNT         1445 a         1821 c         1731 g         1707 t
ORIGIN      
        1 atctgggact gttcgcggtg tatacctggc gggtgggttc gctcttccga tcatccagca
       61 gccggaggat aaacctgctt atgtctccaa tcgcacagga ttggttactc agtttaatgc
      121 gccctcgaat tacggtgtaa cgggcttact tgcccacaat tacctggcag gcgaagaatt
      181 ttataagctg cgtactggtc aggaggttgc actcatctac ggcgatagtc tgataaagcg
      241 ttaccaggtc gcccggattg ataccttcca gaagttaacc ccctcccgtc tgaagagtga
      301 ttatcttgag ctcagcaccg gccgccgcct gagtacatcc cagatgttca aacgttttta
      361 ccgtggcgac gaacacctca ctttccagac ctgtctcgaa aaagatggct tgttgaattg
      421 gggtctgctc ctggtgactg ctgtacccgt cgatagttaa aattaaaaaa gcactcgcag
      481 atggaaggtt ccacctgcga gtgcgccgcc atacacactg acgggtttac tctattgcgt
      541 tacctgatca aagcattcca gtagtcgacg atatcggcat cccaatcgtc ctgtgtcgta
      601 tctggaagct cattgggagc gaagctgggt ataccggtcg ccagagcatc gtcgaccgta
      661 atcaccagcg cttcattcgt tgcatcaagg ttgatcgcat cgaccggaat gagcacccac
      721 cgcccgttga gatcggcaat atcgacccca atctgaacga caacgtagcg gatctcgcca
      781 gtctcaggat tgacaacgat atcttcaatg gaagctaact caccatcgtt ggcatccaca
      841 aagttcatat cgagcacctg gctagccagg acaacgccgg tgagcttacc gtcagccatc
      901 tcactgggtg catccgttgc tgcagcctcg ggcgtacccg ttgtatcagc tgcaggtact
      961 gcttcccagt ggcttcggaa ttcaagatcg tagtcgacgg ctggttctcc cagtaaaggc
     1021 agggcctcac ggtcaaactc agctgaacct tcaagctctt cccggcttac atccagaatc
     1081 cagacgtttt gcggctcgct ctcagaggtg gcatccgcat cagcaagctg caggcggtca
     1141 taaggtaccg cgacaagctt gccgccgatt cccaggaacc caccggcttc aacgatgaga
     1201 tagtcaattt tcttttcctc taaattgagg atcatatctt cggctgtacc gatctgttcg
     1261 tcctccaggt tccatacgtc ataatcgagc tggtttgaaa ccagactcgg gtcgacgaaa
     1321 cccgtcggag gcagaacttc actggtagtg gccggcaagg ttgtggccgg ctcggttacc
     1381 gctggctgtg tttctacgcc aggttcagtt gccgttgggg gctcggtaac ctcagggaca
     1441 tctgtcgcct caggttcttc tgtaaattca ccctctggcg tcatggtcag gtccatggcc
     1501 ggttcggtga acactggagt accttcgggg ttttctgcgg atccgcaggc cgccaacatt
     1561 aataagctca cgatagagcc taacacgatt aacaaatggg tttttctcat ggtctaactc
     1621 cttcttgaat tagttatgta agcacccgca atattcgccg gttacttcgc tgaccataat
     1681 acatacccgt taaggatcta tcaattaggg agttacctga tttgctttaa tggaaatccc
     1741 tactttggtt agttaaatcc cccattgagg cgcgattgtt ttgaacctaa gatggtaagc
     1801 aaaaccccat cccaatgtta ggaggtagat atgaaaaccg aatacagaac ccccgttgcg
     1861 ctctccagct ccacgctaac cggtgaccct gttcataatc tggcgggtga aaccctgggt
     1921 aagctggaag agattatgat cgacctggaa agtggccggg tcgcctatgt cgtgctttcc
     1981 ttcggaggct tcctaggcat gggtgataaa ttgttcgcca tcccatggga agcgatcact
     2041 gtcgacaccg acaagaaagc gatcgtcctc aacgtcagca aagaagtgtt ggagaatgct
     2101 cctggcttcg ataaagatca ctggccagac accagcgaac acgcctggct gacagatatt
     2161 tacgcatact atggattcga tccatactgg agataacccg gcttaagaaa ggatcccatt
     2221 tattcttgag agcaccgggt taacctaata caaatcgggg caggctgatt agtgcctgcc
     2281 ccgatttact atcctcaccg ttcatcttag atgtccacgt ccaaggggtt taagatttct
     2341 ttcgcctcca tttccctgaa gaatcgtctt tctggtaatt cttcttcacg gcagcccacg
     2401 caatcttgtg cgcagtctct tctcgggagc tgtctccgcg ccgttcctga ggtgtatcat
     2461 actgctccca ggcgctgttg aatgcctcac ggtagatatc ctgggcgtga tcaggtagga
     2521 cattctgcac gccatcgggt agctctgaat tcgatttgta aggcatactc acctcctcaa
     2581 tcggatccat tccctttacg gggtgaattg ttattcctcg tgtagatcgc cgatcgttat
     2641 cttggtgttc accagcttct cggggatctg cttggcctca tcgccaattt ctttgacacg
     2701 ctgcttgacc tcatccatct tggccatcgt gcgtgcctgg acatcggaga tcgcctcttt
     2761 tgtccgcatt cctgcttcag agatcccttc acgtagttgg gcttgcgttt ccttgcctgg
     2821 ccgggaggca ttcagcagtg caacagccgt tccggcggcc gcgccagcta taaagacact
     2881 taccagtgta ataaagttag ttttcatctc atatctctcc ggttaatcag actcaaagta
     2941 gataacgatc cagggacctg ttatctctgc ttcactatat cccactcact tgggagagga
     3001 aatggggaat cagcctataa attattaggg atttccccta cctgtctaag gctaagtccc
     3061 aattgtgatt taggcagccg aaatttatat ttagcctatg ggccagggcg gttcaaggcc
     3121 agatgcagtt cataaaatta aaatgaatag agtaatttac gagaccatag gtgaggagac
     3181 ctttagtaat gagtgatgag acgatcgata cagcagtgga aaacccggaa aatatcatcc
     3241 agacgaagaa tgtcactaag gaatttaatg ggacagccgc agttcaggag ctgtcttttt
     3301 atgttcctcg agggaaaata tttggcttta tcggccctag cgggtcaggt aaaacgacca
     3361 cacttcgcat gttgaccggc tattaccatc caaccagcgg agaagtgcgc gtattgggcc
     3421 gttcaccgga acatttcacc cgcgccgagc gtgagaatat tggctacctg ccccagatgt
     3481 tcagtctgta cccttacctg acagtctggg aaaacttaaa ttttgcggct tccatttatg
     3541 gtctgtcact ggcgcggcgc aagcggctgg agaatattct cgagttcgtg gagctgtccg
     3601 gagaccgtga taagctggcc agccagctat cgggtggtat gcagcgccgc ctgaatctgg
     3661 ctgcaacttt gctgcataac cccgacctga ttttcctgga tgaacctacc gctggagtgg
     3721 atccagtctt acggagtaaa ttttgggagc acttccgcga gcttcaggcc cagggtcgaa
     3781 ccatgttcgt gacgacccaa tatgttagcg aagccgctta ctgcgacctg gttggggtga
     3841 ttgaccaggg ccgcctggta atcgtcgata ctcccgcctt actccgttac cgggctttcg
     3901 ggggtgatct cgtggactta cataccgtag ctcccctgga ttatgccacg ctgcaggccg
     3961 tccgcgagct cccctttgta gtctccaggg tctcccaaca atcccctacc agtgtccggt
     4021 tggtggttaa ggaagcgaaa acggacattc cggctttagt agattgggcg cgcgagcgga
     4081 atatggagat cgaggcgatc gaggaatacc tgccgccttt cgatgatgtg ttcgtcaagc
     4141 tggtgaaaga aggtgtagaa aatgggtaac ccgattagcg cgctctggaa agtatttatt
     4201 cgaatatcgg cattcttacg taaggaagtc gtcataatcc tccgccagcc tcagctgctg
     4261 atgactctgg tactggggcc gtttctaatt ttgctggttt ttggattggg ataccgcaac
     4321 gagccgcctg tgcttcgcac cttgtttgta gcccagcctg gcagccagct ggaagagcag
     4381 atcaaggctt atgctccgac gctgggcccc cagctgtctt ttttgggcgt taccggggat
     4441 cgcgaggccg ctatggagag tctccggcgc ggtgatctgg atttagttgc cgtggctccc
     4501 caggatgctg ctgcctcgat ccaggccggt cagcaggctg aattccagct ctatcatgcc
     4561 gacattgacc cgacgcaggc cagctatatc agctatttcg gtgagatata catcgccgaa
     4621 gttaaccgcc gtgtcctgca gtccctcacc gcagagggac aggtgcaggc cgaggattta
     4681 cagcagaatc tgcagtccgc tcgcgcagaa actcaatcgg cagaacaggc gctgcaggcc
     4741 ggcgacgagg tcgctgcccg gcagcacctc gctggattaa cttctaacct ggatcaatta
     4801 tccctggcgg taggcgccag tctggcggtc ttgaacaatg ttgaaggaac ggtaggcagt
     4861 gaatctaacc agcagtccct ggagatcagc cgcttgctca atgatatcca gaccttatct
     4921 gatgagctgg gtgaggcgca aaataccact tctgaccctg gccagtggca aacccgggtc
     4981 gccgagatca attccaattt aaacgatctg gatgctcagc tgcagacctt cacccagttg
     5041 aaaccggagg taatcgtcga gcccttcacc agcactgccc agagcatcgc cgaagtccag
     5101 cctgatgcca ccaatttctt cgcaccggca gtgctcgcct tgctgcttca acacctggca
     5161 gttaccatcg ccggcctctc gatcgtccat gagcgcaatt tcgggactct cgaattgttc
     5221 cgtgtcgctc cgctctccgc aggtgagatc ctgatcggaa aatacctgag ctatctcttc
     5281 tttggagcaa ttgtcgctgc agcccttact gcgctgctgc atctcgccct cggtgtccct
     5341 atgctcggta actggtggaa ttatgccctg gtgatcggag tcttattgtt cacttcgctg
     5401 ggcgtcggct ttgtcatctc cttgatctct cgttccgaca gtcaggccgt tcagtatacg
     5461 atgattgccc tgctggtcag tgtctttttc agcggctttt taatgagcct gaatatgctg
     5521 gcagagccgg tgcgctcggt ctcctggggt atacccacga cctatggcat tatgctgttg
     5581 cgcgcggttg ccctgcgcgg cagttttccc gacctggtcc tcgtcggtat tctggcagcc
     5641 attggtgtgg cccttgctct gttggcctgg cttattctcc gccggatgat ttccaaccag
     5701 tagcaggtag ggaatctcct gattgcacag gtagaagctc tccccattgt gatgcctgga
     5761 ggctgagtct atgatgggat cactaacttt cgaaaaagga gtaagtagat tatgttatcg
     5821 gagaaacaat cccccaaatt ctttcgaccc attctaattt tggctctcac cctggctgtt
     5881 gggttgctgg ctgcctgcag ctcaacgact ctacccgaca ctggaagtct gggtacggcg
     5941 gtggatcagc cgccggaggc tgtcctccag accgttgagc gcttgagcca ggagcttggc
     6001 gtcgctgttg aagagatcga gatcgttgaa ttcgaacagg tggaatggcc ggatgcttgt
     6061 ttaggcttcc cgcaggaagg ccaggcctgt gctcaagttg ttaccccggg attccgggtg
     6121 ctgttggccg tgggcgatca gcagtacgag ttccgcagtg atgagagcgg tacggtgatc
     6181 gtcaagacca ccccggaggc tcctgccccg taaatttttg attgacctcc agcttgcatc
     6241 ggtaacccca ctccaagatt tggcggctgg aataaacgcc gcctgagcga gtgataataa
     6301 ccccggaggc attgttcgct atcgatgaat gcctccgggg ttcgattctc tgtcatacgg
     6361 tcgtcccagt tttgggggta taatttcgac aataaaggac cgaaatgtcc agagtagccc
     6421 ttgtcttccc ttattttcgc acccatgcgc ccacggagat gctctttcct ccccttgggg
     6481 cctcttccct ggctgcgcag ctccgcaagc ttaacatcga atgccatatc tttgactgca
     6541 cctttggaag cttccctcaa ctccaaaagg atttgctggc ttaccagccc gagatcgtgg
     6601 gcatttactc aatggttacg cttagccgga ataccttccg gatagccgag atggtccgga
     6661 agcatctccc cactgcactg ctcgtcgccg gcgggccttt accc
//
LOCUS       DATMXK010000350         4026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_15896, whole genome shotgun sequence.
ACCESSION   DATMXK010000350 DATMXK010000000
VERSION     DATMXK010000350.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15896"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1750
                     /locus_tag="VN363_06500"
     CDS             <1..1750
                     /locus_tag="VN363_06500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559926.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PQQ-binding-like beta-propeller repeat protein"
                     /protein_id="HWQ84198.1"
                     /translation="RARDLHFPNVVKLVAVKEMINQARDPLVRKTIIRNFEREANLLA
                     TLDHRAIPRIYDYFSLDQRSYLVLEHISGKDLEALIADIDDFVFEDRVLAWALELCDV
                     LTYLHNHKPEPIIFRDVKPSNIMITPQDHVVLVDFGIARTFQTGQKGTMIGTEGYSPP
                     EQYRGEATPLADIYALGATLHHILTRRDPRLEPPFSFAERPIRVINPSISIEMEQVLD
                     KALQYNPEDRFQTAQEMKEAFLSIARKTGLLNSVPTPLENQTNLSNASRQIWNFECED
                     ELRGSATYHEGVLYLGSYDNNLYALKADDGKFLWKFPTEGGIVSKPAIFENHIYFGSA
                     DNRLYAVSIASRKPTWTYFTYAPIHSSPLTAEGHVFIGSDDASLHAVNALSGRRAWSV
                     EAGAPVRSTPLIYNDSVYFGAENGEFFCVDFTGRIKWRFKARRAITGSPIINNGVVYF
                     GSVDGTLYAMDAKTGWAIWRFRLNKPTITTPAMIDRLIFTGAIDGCIYCIDINNAKEV
                     WRFQTDHQVTGSPTLYKDSIFCGSVDGHLYCLEYRTGRLRWKYQTQGPITSTPMANND
                     MVYVTSTDHHVYALSA"
     gene            1962..2696
                     /locus_tag="VN363_06505"
     CDS             1962..2696
                     /locus_tag="VN363_06505"
                     /inference="COORDINATES: protein motif:HMM:NF012693.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PP2C family serine/threonine-protein
                     phosphatase"
                     /protein_id="HWQ84199.1"
                     /translation="MQRSHNEDSIFSINASMTSDLNNLPFGLFIVADGMGGHQHGEIA
                     SSIAVRALSGYVINKLCPPIFSYQSSSPDQTIQEIMSQGILDAHRMILKELQGGGTTL
                     SAILLRGGQMTIAHVGDSRAYSISATGEVKLLTRDHSLVRRLIELGQITEEEATSHPQ
                     RNVLYRALGQGEPFEPDVSTYSLPKGGYLLICSDGLWGVIPDDVLAEITLNAPSVQDA
                     CQQMIDAANKAGGPDNISVILVHLPD"
     gene            2731..>4026
                     /locus_tag="VN363_06510"
     CDS             2731..>4026
                     /locus_tag="VN363_06510"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWQ84200.1"
                     /translation="MLNILPDATPDEIRRAYHEAARRLHPDVSEEPNAIEIFLDVQKA
                     YEILTDHEKRIQYDQGILQEVNQHLTSRSTESTTNQVRLQIQYSRTQLSTLGEPQLIY
                     VLLQASTLGNESRYISPPLNVCLVLDRSTSMQGERMDTVKATAIGLVRQLRPEDVLSV
                     VAFSDRADVIVPAVRRLDRADAETKIRMLRTAGGTEIFHGLESAFYEVQRNLSSKRIN
                     QIILITDGQTFGDEFECLRIADLAAARGIRINVLGIGSNWNDNFLDDLAMRTGGRAIY
                     ISRAQDLNDFLKEMFTDMSDVVGDQISLALDLPSGVTLSYAYRLQPDASALPLANSLK
                     LGNLLRESRLVVLLEFTVEPLLRRAYQYEFGRGMFSMQIHSPEQITLTLPIQLTRPVN
                     KLNPDEVPPPEFIQALAYISLYRMQENVHTEISAGNIEAA"
BASE COUNT         1044 a         1023 c          937 g         1022 t
ORIGIN      
        1 ccgcgcaaga gacttgcact ttccgaatgt agtcaaactg gtcgcggtaa aagaaatgat
       61 taaccaggct cgtgatccat tggtacgcaa aacgatcatc cgcaattttg aacgggaagc
      121 aaatctcctg gcaacgcttg atcacagagc cattccccgg atttatgatt atttttccct
      181 ggatcagcga tcctatcttg tactcgaaca catcagcggc aaagatttgg aagccctgat
      241 tgcagatatt gacgattttg ttttcgaaga tcgcgtgctt gcgtgggcgt tggagttatg
      301 tgatgtgctg acctacctgc ataatcacaa gccggagccg atcatcttcc gggatgtgaa
      361 accgtcgaat atcatgatca ccccgcaaga ccatgttgtc ctggtggatt ttggcattgc
      421 ccgtacattt caaaccgggc agaaggggac catgatcggc accgagggtt actctccgcc
      481 agagcaatac cggggagagg ctacaccact ggctgatata tatgccttag gtgcaactct
      541 ccatcacatt ctcacccgcc gtgatccgcg tttggagcct ccgttttcct ttgccgaacg
      601 cccgatccgg gtgataaatc ccagtatatc catcgagatg gaacaagttc tggataaagc
      661 tctgcagtac aatcctgaag atcgtttcca gaccgcccag gaaatgaaag aagcctttct
      721 cagtatcgcc cggaagacgg gtttactgaa cagcgtcccc acacctctcg aaaatcagac
      781 aaatttatcc aatgcatcca gacaaatctg gaactttgaa tgtgaagacg agctgcgcgg
      841 gtcagctacc tatcatgagg gtgtccttta tctgggatct tacgacaata atttatatgc
      901 cctcaaggct gatgacggaa aatttttatg gaaattcccc accgaaggtg gtattgtcag
      961 caaaccggca atattcgaaa atcatattta ctttggctca gcagacaacc gtttatacgc
     1021 agtatcgata gccagccgta aaccaacctg gacctatttc acctatgccc caattcactc
     1081 atctccctta actgccgaag ggcatgtatt tattggctct gacgatgcct cgcttcatgc
     1141 agtcaatgct ttatcgggcc ggagagcctg gagtgtagaa gctggtgcac cagtgcgctc
     1201 gacgcccctg atttacaatg attcggttta ttttggcgcc gaaaatggcg aattcttctg
     1261 tgtggatttc accggacgga ttaaatggcg ttttaaagcc cgccgagcga tcaccggatc
     1321 gcccatcatc aacaatggag tcgtctattt cgggtcggtg gacgggacgc tctatgcaat
     1381 ggatgcaaaa acagggtggg caatctggcg gtttcgcctg aataaaccca cgatcaccac
     1441 cccagccatg atagaccgtt tgatcttcac tggggcgatc gatggctgta tttattgtat
     1501 cgatatcaat aacgctaaag aggtctggcg attccaaacc gaccatcagg taaccggctc
     1561 gcccacattg tataaagatt ctattttctg cggctcagtc gatgggcatc tttactgtct
     1621 cgaatatcgc acgggcagac tgcgctggaa ataccagacc cagggtccca taaccagcac
     1681 acctatggcg aacaacgata tggtctacgt aacctccacc gatcatcatg tatatgcctt
     1741 atcggcctag ctgattaata ttcataaagg aaaactctat gacccgtttc cttgacaaat
     1801 tattcccaaa acgctcaaaa tcaacctccg gtgatgctga accggacccg ttagactcag
     1861 ctgctgaacg tgaccttcaa gtgtcgccac ctatccatat ccaatcaaaa tcgaaactgg
     1921 aaccagaaca atttatagtc ggttgcgccc agtccactgg tatgcagcgc agccacaacg
     1981 aagattcaat ctttagtatt aacgcctcga tgacaagtga tctgaataac ctgccctttg
     2041 gattatttat tgtcgccgat gggatgggtg gtcatcagca tggtgagatt gccagctcca
     2101 ttgcggtccg agcactgagt ggttatgtga ttaataaatt atgcccacca attttcagct
     2161 accaatcctc atccccagac caaactatcc aggaaatcat gagccagggc attcttgatg
     2221 cccatcggat gatcctgaag gagctccagg gaggcggaac aaccctctca gctatattat
     2281 tacgcggcgg gcaaatgacg attgcccatg ttggtgatag tcgtgcttac tctatatctg
     2341 ctaccggtga agttaagctc cttacgcgcg accattcctt ggtccggcgg cttatcgagc
     2401 tgggtcaaat aaccgaagaa gaggcaacca gccacccaca acggaatgtg ctttatcgtg
     2461 cgcttggcca gggagagcct tttgagcctg acgtcagtac atattctctc cctaaaggtg
     2521 ggtatcttct catctgctcc gatggtttat ggggtgtcat ccccgatgat gtcctggctg
     2581 agataacatt gaatgctcca tctgttcagg atgcctgtca gcaaatgatc gatgccgcaa
     2641 ataaagccgg aggtccagat aatatctcag tgatcctggt ccatctccct gattaggtgt
     2701 gaaatagaga cggcagcaaa ttattacgca ttgttaaata tacttccaga cgcgactccg
     2761 gatgaaatcc ggcgcgccta tcatgaagct gcccgccgcc tgcaccctga tgtaagtgaa
     2821 gaacccaatg caattgaaat tttcctggat gtccagaaag cttacgaaat ccttaccgac
     2881 catgaaaaac ggattcagta tgatcagggt attttgcagg aagtaaatca acatctcaca
     2941 agccgctcaa ccgaatcaac gactaaccag gtccgtcttc aaattcaata cagccgtacc
     3001 caattatcga ccctgggtga gccccaactt atatatgtgc tcctgcaagc atctacctta
     3061 ggaaatgaaa gccggtatat cagcccgccc ttgaacgtat gcctggtatt ggatcgttcg
     3121 acatccatgc aaggcgaacg catggatacc gtgaaggcca ctgccattgg gttggtccgt
     3181 cagctgcggc cagaagatgt gctttcggtt gttgcattta gtgacagggc tgatgtaatt
     3241 gtaccggcag tccgccggct ggatcgcgcg gatgctgaga caaaaatccg catgttgcgt
     3301 actgcaggtg gtaccgaaat atttcacggc ctggaatctg ccttttacga ggttcaacgg
     3361 aatttgagca gtaaaagaat taatcagatc atccttatca ctgatggaca gacttttggg
     3421 gatgaattcg aatgtttgcg gatcgcagac ctcgccgctg ccagaggtat acgcatcaac
     3481 gtcctgggga ttggctcaaa ttggaacgat aatttcctgg atgacctggc tatgcggact
     3541 ggtggacgtg caatctacat ttcacgcgcc caggatctta atgattttct gaaagaaatg
     3601 tttaccgata tgagtgatgt ggtaggtgat caaatctcgc tggcattaga tctaccctca
     3661 ggggttactc tctcttacgc atatcgactt caacctgacg catcagcgct gcctttagca
     3721 aactcgctca aattaggaaa tctgctgaga gaatcccggc tggtcgtcct gctcgaattt
     3781 accgtggagc cacttctgcg aagagcctac cagtacgaat ttggacgggg aatgttttcg
     3841 atgcagatcc attcgccgga acagattact cttaccttgc ccattcagct gactcgacct
     3901 gttaataagc ttaatcccga cgaagtacct ccgccagaat ttatccaggc cctggcgtat
     3961 atttcccttt accgcatgca ggaaaatgtc catactgaga tatctgccgg taatattgaa
     4021 gcagca
//
LOCUS       DATMXK010000351         5267 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_44894, whole genome shotgun sequence.
ACCESSION   DATMXK010000351 DATMXK010000000
VERSION     DATMXK010000351.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5267)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5267)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5267
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44894"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..849)
                     /locus_tag="VN363_06515"
     CDS             complement(<1..849)
                     /locus_tag="VN363_06515"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: protein motif:HMM:NF027681.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWQ84201.1"
                     /translation="MPTALITGITGQDGSYLADLLLAKSYTVVGMVRRTSTVTFERLS
                     HIQDDITLIQGDLHDQSSLIDILEQYKPDEVYNLAAQSFVPVSWKQPVLTGEVTGLGV
                     TRLLEAIRLVNPKIRFYQASSSEMFGKAREVPQCETTPFYPRTPYGVAKLYAHWITVN
                     YREEYGIYAASGILYNHESPRRGLEFVTRKITHGAARIKLGRAKELRLGNLDARRDWG
                     FAGDYVRAMWLMLQQDTPEDFVIGTGETHSVREFCQAAFSCLGLDYQNYVVQDERFYR
                     PAEAAQL"
     gene            complement(906..2561)
                     /locus_tag="VN363_06520"
     CDS             complement(906..2561)
                     /locus_tag="VN363_06520"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84202.1"
                     /translation="MEASKSLRNPSWPKWLSLAAGCIIISLLLAWSGSHYQTLQDWPV
                     FLGTSMLAGMLLIGGWWFLKLSEHEALPGQLGLILLLAVVLRLGASAAWFQLIPIYGH
                     GTPEERAGYIMSDAYTRDRTAWELATSEKPLLRAFQGTYRKADQYGGLLFISAGIYRV
                     VGGTQHQPLVMTAISAAFSSLAIIFTWAFSRRAWNPRVANLTAWGLALYPETILLGSS
                     QMREAYTITLAAMAFYGLIKYLQEHTLFSLLWILIPWLAYLAFSPPFAALLLVGLGVT
                     ALFAFNNNSIRRSIPQRWLWIVILILLLLVMIGIWVSWRAFAPDGINNPVDLANWWLR
                     KSASWQAYQSERASGWMQKVFDGTPELLHIPILVGYGILRPFLPASIIATSSSPLWPW
                     ITIWRAAGWTVLLGLLLYASIRAWNRKDHDRIAQALVVFTWLVILVASFRGGGDQDDN
                     PRYRAAFACIQIALAAWAWVSYRQSKDVWFRRALITLGLILIWFIPWYLRRYTTFEWP
                     VENLFLTISLGLASGLLYLAWDIRRSRLQSGVDSSSNEDEDHT"
     gene            complement(2593..3747)
                     /locus_tag="VN363_06525"
     CDS             complement(2593..3747)
                     /locus_tag="VN363_06525"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ84203.1"
                     /translation="MMKICLIGPTYLPSRRANTIQIMKMAQALAECGYEIEVLAPDYK
                     TREPITNSDPRDWNTISQLYGLERQFPITWLPIRPGWRTYDYGWKAVQYAKNHRADLV
                     YTRLPQAAAISSVRGIPTVFEVHDFPQSHAAQNLLRIFLTGRGAQRLVVITHALKADL
                     RSLFGRRLEEPFAIVAPDGVDLARYKSLPDPETAREVLGLENRFTAGYTGHLYPGRGI
                     DFILELAQRLPHVNFLLVGGEDDAVQDWKALAAQRQLANVSLTGFIPNSRLPQYQAAC
                     EILLMPYRTQVAASSGGDIGRYLSPMKLFEYLACGRVILASDLPVLREVLNSDNAILL
                     PPQDLDAWVEAIQNGLQFPEHYTMLARQAQDTAQQFTWKARVLKIFNDTL"
     gene            complement(3771..5132)
                     /locus_tag="VN363_06530"
     CDS             complement(3771..5132)
                     /locus_tag="VN363_06530"
                     /inference="COORDINATES: protein motif:HMM:NF024832.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="oligosaccharide flippase family protein"
                     /protein_id="HWQ84204.1"
                     /translation="MLRALSQSIRAYRDQLLQSQIIRRLVKNSGYLLSATGVSAALSM
                     VQGILTARLLGVANYGLLGSIMLFTSVINKFASFRMSELVIKYIGKHAEDRNDRQAAA
                     VFKAASLAEILASVLAYTLIWYLAPLGATYLAKDASLVNLFRLYGVIVLANLIAESST
                     GLLQIFDRYRKIASLGIVQSIVSLLLITITFLLKGDMQEILIAYIAGKVVSASGLTFL
                     AWQEAKRQWGAGWWRVPLGLLRPQARELAHFAVSTNLSASLNLVNKDSELLWVSFFRG
                     PLETGYYRLALTLANLLQMPVSPLPQATYPELSREVARQDWPNVSHVLREGSRMAGLY
                     TVTASLVLALGGTLIIRYFYGADYLPAYPALIILLLGFLAANFFYWNRIALLALGHPD
                     FPTRVNLALAAVKVAGILLLVPRFGYLANAALLAGYYIFSVGINAWKTGQILRQKAQH
                     VTP"
BASE COUNT         1312 a         1361 c         1449 g         1145 t
ORIGIN      
        1 cagctgggcg gcctccgcag ggcggtaaaa gcgttcatcc tgaacgacat aattctgata
       61 atccaggccg aggcagctga aggcagcctg acaaaattct cgcacggagt gagtctcgcc
      121 ggtgccgatc acaaaatcct ccggagtgtc ttgctggagc atgagccaca tggctctgac
      181 ataatctccg gcaaagcccc aatcccggcg ggcatccaga ttacccaggc gcagttcttt
      241 ggcacgcccc agctttattc gggcagctcc gtgagtgatc ttgcgggtta caaattccag
      301 ccctcgccgg ggcgactcat gattataaag tattcccgat gccgcataaa tcccatattc
      361 ttcgcgataa tttactgtaa tccaatgggc gtaaagcttg gcaaccccgt aaggagtgcg
      421 tgggtaaaat ggggtcgtct cgcactgggg gacttcacgc gctttgccaa acatttcgct
      481 tgatgaggcc tgataaaaac ggatttttgg attgaccagg cgaatggctt ccaacaaacg
      541 ggtaacaccc aatccggtta cttcgcctgt caaaactggc tgtttccagg atacaggaac
      601 gaatgattga gccgccagat tgtagacttc gtcaggcttg tattgctcca ggatatcaat
      661 cagcgagctc tggtcgtgca agtcgccctg gataagggtg atgtcgtctt gaatatggct
      721 gaggcgttca aaagtcactg tgctggtacg gcggaccatg cctactacgg tgtagctttt
      781 cgcaagcagc aggtcagcca gatatgatcc atcttgtcca gtgatgcccg tgatcagggc
      841 ggtaggcata cgatttcctc actttaaaat tcaatcgctg aattataatc tgcggtttgt
      901 gcgggtcaag tgtgatcttc atcctcgttc gaggagctat caaccccgga ctgtaaccgg
      961 ctgcgacgaa tgtcccaagc aagatatagc aaaccggagg ccagaccgag actgatcgtg
     1021 aggaacaagt tttcgaccgg ccattcaaag gttgtgtagc ggcgtaagta ccagggtata
     1081 aaccagatca agatcaagcc cagcgttatg agtgcccggc gaaaccagac gtcttttgat
     1141 tgtctgtatg agacccacgc ccaggctgcc agtgcgattt gaatacaggc gaacgctgcc
     1201 cggtagcgag gattatcatc ctggtcgcct cccccgcgaa aagacgccac caggattacc
     1261 agccaggtaa acacaaccag ggcttgagca atccgatcgt gatccttgcg gttccaggcg
     1321 cggatcgaag cgtagagtaa cagtccgagt aataccgtcc aacctgcagc ccgccagatt
     1381 gtgatccagg gccagagagg tgagctgctt gtagcgatga tcgacgcggg taagaatggc
     1441 cgcagtatcc catatccaac taggatcggg atatggagga gctcaggcgt gccgtcgaag
     1501 accttttgca tccagccgga ggctcgctcg ctctggtaag cctgccagga tgcagatttg
     1561 cgcagccacc agttggcaag atcgacagga ttattgatcc cgtctggagc aaaggctcgc
     1621 cagctgaccc agataccaat cataaccaga agaagcagga tcaggatgac aatccagagc
     1681 cagcgctgcg gaatactccg ccgaatagaa ttgttattaa aggcgaataa agccgtaaca
     1741 cctaacccga ccagcagtaa ggcagcgaag ggtggggaaa aagccagata agccagccag
     1801 ggaataagga tccacagcag gctgaataaa gtatgctcct ggaggtattt tattaagcca
     1861 tagaatgcca tcgctgccag agtgatggtg taggcttcgc gcatttgaga gctgcccagc
     1921 aggatcgttt ccgggtataa ggctaatccc caggcggtga ggttggcgac ccggggattc
     1981 caggctcgcc tggaaaaggc ccaggtaaaa ataattgcca gcgaagaaaa tgccgcggaa
     2041 attgcggtca taaccagcgg ctggtgctgg gttcctccca caacacggta tattccggcg
     2101 cttatgaaaa ggagaccccc atattgatca gccttgcggt atgtcccctg aaaggctcgg
     2161 agcaggggtt tttcggatgt ggccagctcc caggctgtcc tgtcccgtgt ataggcatcc
     2221 gacatgatat agccagctcg ttcttcagga gtgccatgcc cgtagatggg gattagctga
     2281 aaccaggctg cgctggctcc caggcgaagc acaactgcga gtaagaggat caatccaagc
     2341 tgaccaggga gtgcctcgtg ctcgctaagc ttaaggaacc accagccacc tatcaggagc
     2401 ataccagcca gcatggacgt gcccaggaat accggccaat cctggagcgt ctggtaatga
     2461 cttccagacc atgccaaaag cagggaaatg attatacagc cagcggccag gctgagccat
     2521 tttggccagg aggggttacg cagagatttt gaagcttcca tttatcaagg gacaaacttt
     2581 ttcggtaatc atttataagg tgtcgttgaa tattttcagg acgcgagcct tccaggtaaa
     2641 ttgctgggca gtatcctgtg cctgcctggc caacattgta tagtgctctg ggaattgtaa
     2701 tccattttgg atcgcttcca cccaggcatc tagatcctgc gggggaagta aaatcgcatt
     2761 gtctgagttg aggacttcac gtagtaccgg tagatcgctg gcaaggatca ctctgccaca
     2821 cgccaggtat tcgaataatt tcattggact gagataacgc ccgatatcgc cgccagagga
     2881 agctgccacc tgcgtccggt atggcatcaa caggatctcg caggctgcct gatactgagg
     2941 aagccgggaa ttggggataa aaccggtcag gcttacattt gccagctgtc tttgagctgc
     3001 gagtgctttc caatcctgca ctgcgtcgtc ttcgccgcct accagcagaa aatttacgtg
     3061 gggcaggcgc tgggctaatt ccagaatgaa atctataccc cgaccgggat ataaatgccc
     3121 tgtataaccg gctgtaaagc gattctcaag accgagcacc tcccgggcgg tctcagggtc
     3181 aggtaaggat ttatagcggg ccaggtcgac cccgtccggg gcgacgattg cgaaaggctc
     3241 ttcgaggcgc ctaccaaata atgatctcag gtcagctttt aatgcatggg taatgactac
     3301 caatcgctgc gctcctcttc cggtgagaaa tatccgcagg agattttgcg cggcatggct
     3361 ttgtggaaaa tcatggacct caaaaacagt cgggataccc cgcacactgg atatggcagc
     3421 cgcctgtggc aagcgggtgt atacaaggtc tgcccggtgg tttttggcat actgcacagc
     3481 tttccagccg taatcatacg tgcgccatcc cggccggatc ggaagccagg ttatggggaa
     3541 ctgtcgctcc agtccataaa gttgagagat ggtgttccaa tctcttgggt ctgagttcgt
     3601 tattggttca cgggttttat aatcaggcgc caggacctcg atctcatatc cacactcagc
     3661 cagagcttga gccatcttca taatctggat ggtgttggcc cggcgagagg gtaaataagt
     3721 tggtccgatc aggcagattt tcatcatgtt gaggcaagct tatcatgtct ttatggggtc
     3781 acgtgctgag ctttctggcg caggatctgg cctgttttcc aggcattgat gcccacactg
     3841 aagatatagt agccagccag tagagctgcg ttagcgagat agccaaaacg aggaaccagg
     3901 agcaagattc ccgcgacctt taccgctgcc aaagccagat tgacccgggt gggaaagtct
     3961 ggatgtccaa gcgcgagcag cgcgatcctg ttccaataga agaaatttgc agccaaaaaa
     4021 ccgagcaata agataatcag ggcggggtag gcaggcagat aatccgcgcc ataaaaataa
     4081 cggatgatca gagtaccgcc gagagccaga acgagtgagg ctgttaccgt gtacaaccct
     4141 gccatgcgcg agccctctcg caatacatgg ctgacattcg gccaatcctg gcgggcgact
     4201 tcgcgggaaa gctccgggta ggtagcctgt gggagagggc tgaccggcat ttgaagcaga
     4261 tttgccaggg tgagcgccag gcggtaatag cccgtttcta atggaccccg aaagaaggac
     4321 acccagagca gctcgctgtc cttattgacc aggtttaacg aggcgctgag gttggtgctg
     4381 accgcaaagt gggccagctc gcgggcttgt ggacgaagca gacccagtgg cacacgccac
     4441 caacctgctc cccactggcg cttcgcctct tgccaggcca ggaaggtgag gccagaggca
     4501 ctgacgactt tcccagcgat gtacgcaatc aggatctctt gcatatcccc cttgagcaag
     4561 aatgtaatcg taattaatag taatgaaaca atgctctgca cgatcccgag cgaggcaatt
     4621 tttcgatagc gatcaaaaat ttgcagcagg ccggtagaac tttccgcgat caggttcgcc
     4681 aggacaataa ccccatacag gcggaataaa tttaccaggc tggcatcttt ggccaggtag
     4741 gtagctccca gcggtgccag ataccagatc agagtatagg ccaggacaga ggccagtatt
     4801 tctgccagac tggcagcctt gaaaacagct gcagcctgtc ggtcatttcg atcttcggca
     4861 tgtttaccga tatatttgat gaccaactca gacatgcgga aggaagcgaa tttgttgatc
     4921 acactcgtaa agagcataat gctgcccagc agcccataat tagcaacccc cagcagccgg
     4981 gcggtgagaa tgccctgaac catgctgagg gcagctgaga cccctgtggc gctcaggaga
     5041 tagcctgaat ttttgaccag acgccggatt atctggcttt ggagcaattg atcgcgatag
     5101 gcccggatcg attgggacag ggcacgcaac atagctgtta aattgtgacg atctggctgg
     5161 cccaagatct ttccgccagg taccaatcca ctaaattttg aataccttct tccaggccta
     5221 ctaatggctc ccatccgagc aggcggcgag ccttttctac actggcc
//
LOCUS       DATMXK010000352         3912 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_117962, whole genome shotgun sequence.
ACCESSION   DATMXK010000352 DATMXK010000000
VERSION     DATMXK010000352.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3912)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3912)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3912
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117962"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..874
                     /locus_tag="VN363_06535"
     CDS             <1..874
                     /locus_tag="VN363_06535"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ84205.1"
                     /translation="GCDNRCTFCVTTLARGAGRSRPVAEILADIRAALQPGVSQAREI
                     VLTGVHLGSWGHDFEAPSRLHDLIQVILTETDVPRLRLSSLEPWDLDETFFELWSDPR
                     LCRHLHLPLQSGSAATLRRMARKTTPTSFARLLDTARQAIPHVAITTDIITGFPGESE
                     EEFNESLAFIQAMHFAGGHVFTYSARPGTAAARMPAQVPHELRKARNAQMRAVLEVTG
                     EHYRDSFLGQVLPVLWESAQALDSSGWAVSGLTDNYLRVQAYTPRHLWNEITPVRLVA
                     PDPIGLRGQLLEKT"
     gene            935..1279
                     /locus_tag="VN363_06540"
     CDS             935..1279
                     /locus_tag="VN363_06540"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_002213087.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine triad nucleotide-binding protein"
                     /protein_id="HWQ84206.1"
                     /translation="MTDCIFCKIISGEIPGEKLYQDDSVTAFRDLHPVAPTHVLIVPN
                     KHIPSVNEVSPEDESTMGHLYTVARQLAVQEGIQEQGYRLIVNSGPNAGQEIYHIHLH
                     LIGGRRMRYPMG"
     gene            1309..2109
                     /locus_tag="VN363_06545"
     CDS             1309..2109
                     /locus_tag="VN363_06545"
                     /inference="COORDINATES: protein motif:HMM:NF012797.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008173 - RNA methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0001510 - RNA methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA methyltransferase"
                     /protein_id="HWQ84207.1"
                     /translation="MITSNQNPKIKWVRQLQNQARARKEETVFVVEGVRLAEEALNAG
                     WKAELVLHSVNLSARGQAVVSGFAERGGPVEEVMEGVLEAASDTQTPQGILVVVHQST
                     YPLPQNLNFALIADEIRDPGNLGAILRSAAAAGVQAVFLNPGCADPFSPKVVRSAMGA
                     HFRLHLAALDWPAIQNQVTAARLHVALAAAGTGMVYTACDFHRPSAIIIGGEATGAGA
                     QAHNLAETLVHIPMAGSTESLNAAAAAAILLFEVVRQRGQPVETSPTT"
     gene            2106..3332
                     /locus_tag="VN363_06550"
     CDS             2106..3332
                     /locus_tag="VN363_06550"
                     /inference="COORDINATES: protein motif:HMM:NF017019.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF711 family protein"
                     /protein_id="HWQ84208.1"
                     /translation="MRIRAITYFFSPDPELSPRSIHQAGIFSKRAQAAFEAGGYEVQT
                     TRLATSPFPHWLPEVNEKALLQAAQAFEAAAAEQGIDYLSFGPALPDYPPTYRLIPPV
                     LAETSSIFFSGVLTTPHGGISLPAVQASGEIIQTVAGFDANGFANLRFAALANVPPGA
                     PFFPAAYHAGGSPVFSLACEAADLAVEAFSQADTLAAARRSLVASLEQHAGALTEIAI
                     RLQNETHIPFGGLDLTPAPFPDLEHSLGTALERLGVPNLGQPGSLAAAAILAATLDQA
                     TFARCGFNGLMLPVLEDALLARRAAEGSLSLKDLLLFSSVCGTGLDTVPLPGDATAQQ
                     LAAVLLDVAALAQRLNKPLTARLMPIQGKKAGDPTNFDFAYFANSRVLALDAAPLTGF
                     LAGEENFDLQPRQSQV"
     gene            3426..>3912
                     /locus_tag="VN363_06555"
     CDS             3426..>3912
                     /locus_tag="VN363_06555"
                     /inference="COORDINATES: protein motif:HMM:NF013866.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ84209.1"
                     /translation="MLAAEERLEFPTSSGLEIVGTIYRPMDREPPWPGVLLLHMIYGQ
                     RSDWDEFARTLAQSGVVALAIDLRGHGETGGDMDWESARQDLSQVWESFVSRPEVSPD
                     LTAVIGASMGANMAVLLGTDRPEVRALGLLSPGLNYYGVQIAEPLAAYGDRPVLIVAS
                     QE"
BASE COUNT          800 a         1170 c         1092 g          850 t
ORIGIN      
        1 cgggtgtgac aatcgctgta cattttgcgt cacgaccctg gcgcgaggcg ctgggcgcag
       61 ccggccggtt gccgagatcc tggcagatat tcgggccgcg ttacaaccgg gcgtaagcca
      121 ggcgcgtgag atcgtgctta ccggagtgca tctgggctcc tggggacacg atttcgaagc
      181 cccttcccgc ttgcacgatc ttatccaggt tatcttgact gaaacagacg ttccccgctt
      241 gcgcctctct tcgctggaac cctgggacct ggacgaaaca ttcttcgaat tgtggtcaga
      301 tccgcgcctg tgccggcatc tgcacctgcc actccaatcc ggcagcgccg cgaccttacg
      361 gcggatggcg cgcaagacca ccccgaccag cttcgccagg ctgctggata ctgcgcgcca
      421 ggcaattcca catgtcgcaa ttacgaccga catcatcacc ggattccccg gtgagagcga
      481 agaggaattc aacgaaagcc ttgcgtttat acaggccatg cacttcgccg gtgggcatgt
      541 attcacatat tccgcccgac caggcacggc ggcggcgcgc atgccggccc aggtgcctca
      601 cgagctgcgc aaagcccgca atgcgcaaat gcgcgcagtc ctggaagtca ccggggagca
      661 ttatcgagac agcttccttg gtcaggtgct gcctgtgctt tgggaatccg cccaggcgtt
      721 ggattcctca ggttgggcgg tgagcgggct gactgataac tacctgcgtg tacaggccta
      781 taccccccgc catttatgga atgaaattac tccggtgcgc ctcgttgcac ccgacccgat
      841 tggcttgcgc ggccagcttc ttgaaaagac ttaagatttt actgagcgat caaatatttg
      901 atcatccctt aattcatatt ctcatggagg ggaaatgact gactgcattt tctgtaaaat
      961 catctcaggg gagattccag gcgagaagtt gtaccaggat gattcggtga ccgctttccg
     1021 tgatctccat ccggtagccc ccacccacgt attaattgtc cccaataaac acatcccatc
     1081 cgtgaatgag gtatctcccg aagatgagag cacaatgggc cacctttaca cggttgctcg
     1141 ccagctggcc gtccaggaag gcattcagga gcaaggttac cgcctgatcg tcaatagcgg
     1201 tcccaatgca ggtcaggaga tttatcatat ccatctgcat ttgattggcg ggcggcggat
     1261 gcgctacccc atgggttagg ctatccttca gaatagacaa tatttggaat gatcacttcg
     1321 aatcaaaatc cgaagatcaa atgggtgcgc caattgcaaa atcaggcgcg ggcgcgcaaa
     1381 gaagagacgg tctttgtggt tgaaggcgtc cgcctggcag aagaagcgct caatgccggg
     1441 tggaaagcag aactggttct tcattcggtt aatttaagcg ctcgcggcca ggcagttgtt
     1501 agtggatttg cggagcgggg cggtccggtc gaagaggtca tggaaggtgt tctggaagct
     1561 gccagcgata ctcagactcc ccagggaatt ctggtcgttg tccaccaatc cacgtatcca
     1621 ctcccacaaa acctcaattt tgctttgatc gcagatgaga tccgcgatcc tggcaacctg
     1681 ggcgctatcc tgcgcagcgc tgccgccgcc ggcgtgcaag cagttttcct gaaccccggt
     1741 tgcgccgacc cattctcacc caaagttgtc cgatcggcca tgggggctca ttttcgcctc
     1801 catctggcag ccctggattg gccagccatt caaaaccagg tcacagcagc ccgcctgcat
     1861 gtcgctctgg ctgcggccgg aacagggatg gtgtataccg cctgtgattt ccaccgtccc
     1921 tcggcgatca ttatcggcgg tgaagccacc ggcgcaggcg ctcaagcgca taatctggca
     1981 gagaccctgg tccatatccc aatggcaggc agcacagaat ccctgaatgc agccgcagca
     2041 gcggcgatcc tccttttcga agtcgtccgc cagcgcggcc aacccgtaga gacatcacca
     2101 acaacatgag aattcgcgcc attacttact ttttttcgcc cgatccagag cttagcccga
     2161 gaagcatcca ccaggctgga atattttcaa aacgcgccca ggctgccttt gaggccggcg
     2221 ggtatgaagt ccagacaacc cgcctcgcca catcaccctt cccccattgg ctgcctgagg
     2281 ttaatgagaa ggcattactg caagccgcac aggcttttga agctgcagcc gcagagcagg
     2341 gcatcgacta tctctccttt ggccctgcct taccggatta tccgcccact tatcggctca
     2401 ttcctccagt cctggcagag accagcagta tattcttttc cggcgtgctg accaccccac
     2461 acggtgggat cagcctgcca gccgttcagg ccagtggtga gatcatccag accgtggcag
     2521 gatttgatgc caatggattc gccaacttgc gtttcgccgc cctggcgaat gttcctccgg
     2581 gagcgccgtt tttcccggcc gcctatcacg ctgggggctc gcccgttttc agcctggctt
     2641 gcgaagctgc cgatctggcc gtggaagcat tcagtcaggc cgatacgctg gcagccgctc
     2701 ggcgaagcct ggtagcgagc cttgaacagc acgctggagc attaacagag atcgcaattc
     2761 gcctgcaaaa cgagacccac atcccatttg gcgggctgga tttgacccca gctccttttc
     2821 cggacctgga acactctctt ggaaccgctt tagagaggct gggtgtgccc aatttaggac
     2881 aacccggctc gctggccgcg gcagctatcc tggctgccac tctggaccag gcgacctttg
     2941 cccgctgtgg cttcaacgga ttaatgctgc ctgtcctgga ggatgctctg ctcgcccggc
     3001 gcgccgcgga aggcagcctg agcctgaaag atctgctgct gttctccagc gtttgcggca
     3061 ccggtctgga tacggttcct ctacctggcg atgctacagc ccaacagctt gcggcagtct
     3121 tactcgatgt cgccgccctg gcccagcgtc tgaacaaacc cctgacggcg cgcttgatgc
     3181 ccattcaggg caaaaaggct ggcgatccta ctaatttcga ttttgcctat tttgccaaca
     3241 gccgggtgct cgctcttgac gctgcgccgc tgaccggatt tttggccggg gaggagaatt
     3301 tcgatctcca accgcgccag tcccaggttt gaacccttaa tttgagaata ctcaatcacc
     3361 gataccttgt tgtactgctg atcattctgg ggaccctgtt cttaccggcc tgccaggcag
     3421 aaggaatgtt ggctgcagag gagcgcctgg aatttccgac cagctcaggc ctggaaattg
     3481 tcggcacgat ctaccggcct atggacaggg aacctccctg gcccggggtg ctcttgttgc
     3541 atatgatcta tggtcagcgg agcgattggg atgaatttgc cagaacgctc gcccaaagcg
     3601 gggtggtcgc cctggcaatt gacctgcgcg gccatggcga aactggcgga gatatggact
     3661 gggaatcagc ccgccaggac ctcagccagg tatgggaaag ttttgtaagc aggccagaag
     3721 tcagtccgga tctaaccgct gtgatcggcg cgagcatggg cgcgaacatg gcagtcctgt
     3781 tgggaaccga ccggcctgaa gtccgggcac ttggtctgtt atcgccgggc ctgaattatt
     3841 atggggttca aatagccgag cccctggcag cctacggcga ccgcccggtt ttaatcgtcg
     3901 ccagccagga ag
//
LOCUS       DATMXK010000353         2748 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_145502, whole genome shotgun sequence.
ACCESSION   DATMXK010000353 DATMXK010000000
VERSION     DATMXK010000353.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2748)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2748)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2748
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_145502"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(197..1066)
                     /locus_tag="VN363_06560"
     CDS             complement(197..1066)
                     /locus_tag="VN363_06560"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559076.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0016051 - carbohydrate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="N-acetylneuraminate synthase family protein"
                     /protein_id="HWQ84210.1"
                     /translation="MPEVVQIGNKLVGNGQPCFITAEIGINHNADLTITKQLIDVASL
                     AGCDAVKFQKRTPEKAVPVEYQNVMRETPWGIMSYLDYRRKMEFDRQAYQEIDRYCKE
                     KGILWYASCWDEESVDFMEEFEPPCYKAASASLTDTDLLLKMYRTGRPLIISTGMSTS
                     DEIGQAVKLLGTDHLLIAHSTSSYPCPPEELNLRMILTLQEHFPVPVGYSGHEVGLQT
                     TLAAVTLGASFVERHITLDRAMWGSDQAASVEPGGVMRLVRDIRVIERALGDGVKQVY
                     ESEMKARQKLRRK"
     gene            complement(1075..2262)
                     /locus_tag="VN363_06565"
     CDS             complement(1075..2262)
                     /locus_tag="VN363_06565"
                     /inference="COORDINATES: protein motif:HMM:NF014409.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acylneuraminate cytidylyltransferase"
                     /protein_id="HWQ84211.1"
                     /translation="MKCLAIIPARGGSKGIPRKNIRPLAGKPLLAYNIEAARASRYIQ
                     RVVVSTDDPEIARVAQQSGAEVVWRPAEISGDNASSESALLYTLTTLQNQEAYAPELL
                     VFLQCTSPLTLAEDIDGTITALLDQDADSALAVIPFHYFLWQRGVDSAGQPDASGINH
                     TKTVRPLRQEREPQYLETGAVYVMRVPGFQQAQHRFFGRTALYEMPGERRLEIDDPVD
                     FQVAEVLIAAQRQTRQLERLPKPVKALVIDFDGVFTDNTVIVNEDGREAVICSRSDGW
                     GISQLRKTGLQLLILSTETNPVVQARADKLKIPCLHGLSEKGLTLQNWLVEQQIDPAY
                     VVYLGNDQNDLTCMAQVGCAVAVADAHPEALAQADIILSHTGGNGAVRELCELILKST
                     GTI"
     gene            complement(2281..>2748)
                     /locus_tag="VN363_06570"
     CDS             complement(2281..>2748)
                     /locus_tag="VN363_06570"
                     /inference="COORDINATES: protein motif:HMM:NF014893.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWQ84212.1"
                     /translation="LSHPLDYLRWLCGEIRECFAFTGRTGNLELDVEDTAEISLRFET
                     GILGSLHLDYNQRPSAHVLEIVGSTGTLKWDNTSGGVSLYQAGSDTWQEFPAPAGFER
                     NHLFEREIRHFLAVARGDEEPICTLEDGWRDLEWAVLALHSAATGQSVRRGDI"
BASE COUNT          601 a          775 c          734 g          638 t
ORIGIN      
        1 ttccagcagc gccttgcgtt cttcatcgga aacgaccagg ttgaccagat ttcccccgta
       61 gggagtgatc aacttgccgt ttttcttcga ttgtgacata gattgttctc cttaattcct
      121 tatcttaaga ctgttgtatc atcaaaaaat gcgtatgatc gtgccaggaa atattcaacc
      181 tcaggcaatc cccggcctat tttcggcgta gtttctggcg ggctttcatt tcgctctcgt
      241 aaacttgttt gacgccatct cctagagcgc gctcgatgac ccgtatatcg cgcaccagac
      301 gcatcacacc cccaggctca acggatgctg cctgatcgct gccccacatc gcccggtcca
      361 gggtaatatg gcgctccacg aagctggcgc ccaaagtgac cgctgccagg gtagtctgca
      421 agcctacttc gtgtccggaa tatcctaccg gcaccgggaa gtgctcctgc aaggtaagga
      481 tcatgcgcag gtttaattcc tcaggcgggc agggatagga gctggtgctg tgagcaatca
      541 acaagtggtc agtcccgagc agtttcactg cctgaccgat ttcgtcagac gtggacatgc
      601 cggtagagat gatcaagggg cgaccggtcc ggtacatttt aagcagcaga tcggtatcgg
      661 ttagcgaggc tgaagcggcc ttgtagcagg gcggctcaaa ttcttccatg aaatcgaccg
      721 attcttcatc ccagcacgag gcgtaccaca ggattccttt ttccttacag tagcggtcga
      781 tctcctggta agcctggcga tcgaattcca ttttccgccg gtaatccagg tacgacatga
      841 tcccccaggg agtctcacgc attacatttt gatattcgac cggtacagct ttttcggggg
      901 tccgcttttg gaatttaacg gcatcacagc ctgccaggct ggcgacgtcg attaattgct
      961 tggtgatggt cagatcggca ttgtgattga tcccaatctc agcggtgatg aaacaaggtt
     1021 ggccattccc aaccagcttg ttaccaattt gaacgacttc gggcattctg actcctagat
     1081 agtccctgta gatttgagga tgagctcgca aagttcgcgc actgcgccat tgcctccggt
     1141 gtgactcaga ataatatcgg cctgagccaa tgcctcgggg tgggcgtcgg caacagcaac
     1201 cgcgcagccc acctgcgcca tgcaggtcag gtcattttga tcattgccca gatagaccac
     1261 atacgctgga tcaatctgct gctccaccag ccagttctgt aaagtgagac ccttctcgct
     1321 caacccgtgc aagcagggaa ttttcagctt gtcagcccga gcctgaacaa ccggattcgt
     1381 ttcggtgctg aggattaaca gctgcaggcc agttttacgc agctgggaga tcccccagcc
     1441 atcgctgcga ctgcagatca ccgcctcgcg gccatcttca ttgacgatga cagtattatc
     1501 ggtgaatacg ccgtcgaaat ctatcaccag ggctttcacc ggtttgggca atcgctccag
     1561 ttgccgggtt tggcgctggg cggcaatcag aacttcggcc acctggaaat ccacggggtc
     1621 atcaatttcc agacggcgct cgccaggcat ctcatataaa gctgtccgtc cgaaaaagcg
     1681 gtgctgagct tgctgaaaac ccgggacgcg catcacatag actgcgccag tctccaggta
     1741 ctgcggctcg cgttcctggc gcagtggccg cacggtttta gtgtggttta tcccactcgc
     1801 atctggctgg cccgcggagt ccactcctcg ctgccagaga aaataatgaa aagggatcac
     1861 ggccagggca gaatctgcat cttgatcgag cagagctgta atcgtgccat cgatatcctc
     1921 ggccagggtt aagggggagg tgcactgcaa gaaaaccagc aattcaggtg cgtatgcttc
     1981 ctggttctga agcgttgtga gggtatataa aagggctgat tcggatgagg cgttatcgcc
     2041 gctgatttct gctggtcgcc agacgacctc cgcaccggat tgttgagcaa cccgggcaat
     2101 ctcaggatca tcggttgaga caaccacgcg ctggatgtag cgtgaagcgc gggctgcttc
     2161 gatgttataa gccaacaggg gtttgcccgc cagagggcga atatttttac gcgggatacc
     2221 tttggatcca ccgcgcgcag gaataatagc cagacacttc atcgttatga tcttactatt
     2281 ctaaatatca ccccgccgga ctgactgccc ggtagcagcc gagtggaggg ccaggaccgc
     2341 ccattccagg tcgcgccagc cgtcctccag agtgcagata ggctcctcgt cgccccgggc
     2401 tacagccagg aaatggcgga tctctctctc gaaaaggtga ttgcgctcaa atccggcagg
     2461 cgccgggaac tcctgccagg tgtctgatcc agcctggtaa agcgacacac ccccgctggt
     2521 attgtcccat ttcagggttc cggtactgcc cacaatttcg agtacatggg cagacggtcg
     2581 ctgattataa tctaagtgca ggctgccgag gataccagtt tcaaagcgca ggctgatttc
     2641 agccgtatcc tccacatcca gctccaggtt ccctgttcgc ccggtgaacg caaaacattc
     2701 ccgaatttct ccgcacagcc agcgcaagta atccagggga tggctgag
//
LOCUS       DATMXK010000354         3344 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_58394, whole genome shotgun sequence.
ACCESSION   DATMXK010000354 DATMXK010000000
VERSION     DATMXK010000354.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3344)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3344)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3344
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58394"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..142)
                     /locus_tag="VN363_06575"
     CDS             complement(<1..142)
                     /locus_tag="VN363_06575"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559368.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_except=(pos:complement(83..85),aa:Sec)
                     /transl_table=11
                     /product="selenide, water dikinase SelD"
                     /protein_id="HWQ84213.1"
                     /translation="MPAPTGKEIRLTSLSSCAGUASKLSAEALAQVLRPIQGMFQAAD
                     YPD"
     gene            complement(224..1291)
                     /locus_tag="VN363_06580"
     CDS             complement(224..1291)
                     /locus_tag="VN363_06580"
                     /inference="COORDINATES: protein motif:HMM:NF013324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005743 - mitochondrial inner membrane
                     [Evidence IEA];
                     GO_component: GO:0035632 - mitochondrial prohibitin
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prohibitin family protein"
                     /protein_id="HWQ84214.1"
                     /translation="MNFANAFQGLVALLWLAFIGVAVLIVVRAYRNKRSKPLNTILVV
                     LGIAAGVVTIVSMGLVFVEPEERGVVISAVDPTGIRREALQPGLRWIIPLVERVERYP
                     ISRQIYTMSADPLEGEGQIEDTVIARTSDGQEIYVDASVLYAVDPEKVVDLHIIWQKR
                     YTLELVRPLARGIIRDAVSQFGVEEVYSSRRQDMASMITIQLAEKYAANGLILDDFIL
                     RNITFSQEYAASVEQKQIAEQQAQQARFVVEQRRQEAEQARQVAEGRADAQVIEAQGR
                     ADSRIIEAEAEKQALELLAEALAKNPDLLTYEYIIKLSPGIQVMLVPNDAPFLLPLPT
                     LAAEAGVTSVPAPTPQPAPAP"
     gene            1457..2146
                     /locus_tag="VN363_06585"
     CDS             1457..2146
                     /locus_tag="VN363_06585"
                     /inference="COORDINATES: protein motif:HMM:NF021903.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84215.1"
                     /translation="MQTSTGLVQEIVLGADGLQAAWISCPPTSLPAPGQYLSAWSPMD
                     PLAPLAATLFAGIIQEDRFLALSPLPASWQPGTRLALRGPLGHGFTLPVTARRVALAA
                     FGQSCERLMPLAHLALQIEASVALFTDGWLPPLPAALEANPLSSLPEALSWADYLAVE
                     ADHTTLPQLRTKLNVPPGEHLPCPAQVLFRVDMPCAGLAVCGACWTPARRGLLYACSD
                     GPVFTLDDLEW"
     gene            2169..2924
                     /locus_tag="VN363_06590"
     CDS             2169..2924
                     /locus_tag="VN363_06590"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L deacetylase family protein"
                     /protein_id="HWQ84216.1"
                     /translation="MNILAFFAHPDDETMLAGGTLALLAHTGANVHYLCATRGEGGET
                     GEPPLCQPEQLGEVREDELACAVQALHGASLDFLGYADPRVGPDEALFAYTNQPGEVV
                     HHLLAEIRENDIQAIVTHGSNGEYGHPAHTLSYRAARQAVESLGAAAPLLYCAMASFP
                     DHPRPRLMNKDQSAHFVLDITPALPAKTRAALCHQTQHALFVRRPSQDAGRMLSVPEV
                     ILTLEGLHRAFPATAGEPQDELSDRLKPWRRTP"
     gene            2943..>3344
                     /locus_tag="VN363_06595"
     CDS             2943..>3344
                     /locus_tag="VN363_06595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84217.1"
                     /translation="MAKYDLALSPTLMNAAGSLGFAPPGHAAIQWERFGAFVTNPISL
                     GRRQPANSAGQISFSGGFLLHTGYPNPGFETTLRRFRAAWSHAPVPVLVHLIPRTAAE
                     LAAMIPPLESCDNLAGMEIGIAPDITLEEVVA"
BASE COUNT          663 a         1050 c          903 g          728 t
ORIGIN      
        1 ggtccgggta atctgcggcc tggaacatac cttgaattgg gcgcaagacc tgcgccagcg
       61 cttcagcgct taatttcgac gctcaaccgg cgcagctgga aaggctggta aggcggatct
      121 ctttaccggt tggggctggc atttgagcca tttgatctcc ttctaaaaat aactcctggc
      181 acaggatcat gctgccagca gtctgaggcc aatttttact ttctcacggc gcaggtgctg
      241 gttggggtgt tggcgccggc acagacgtca caccagcctc cgccgccaat gtcggcaggg
      301 gcaagagaaa cggcgcatcg ttcggaacca gcatcacctg gatacccggt gaaagcttga
      361 tgatgtactc gtaggttagt aaatccggat tttttgccag ggcttcagcc aataattcca
      421 gggcttgctt ttcagcctcc gcctcgataa tccgcgagtc ggctctacct tgcgcctcaa
      481 tcacctgggc atcggcgcgg ccttccgcta cctggcgtgc ctgctccgct tcctggcggc
      541 gttgctcaac cacaaaccga gcctgttggg cctgctgttc ggcaatttgc ttttgttcaa
      601 ccgaagcagc atattcctgt gagaatgtta tattgcgcaa aataaaatca tccaggatca
      661 gaccattcgc agcatacttt tccgccagtt ggatagtgat cattgacgcc atatcctggc
      721 ggcgagagct atagacttct tccacgccaa attgggaaac ggcatcccgg atgatacccc
      781 gcgccagcgg gcgcaccaac tccaaagtat agcgtttctg ccagatgata tgcagatcaa
      841 ccaccttctc agggtcgacc gcgtagagca ctgaagcatc aacatagatc tcttgcccat
      901 ccgaagtgcg ggcaatgacc gtatcttcaa tctggccttc cccctccaat gggtctgctg
      961 acatggtata gatttggcga gagatgggat accgttccac ccgctccacc aaggggatga
     1021 tccaacgtaa accaggttgt aaagcttccc tgcgaatacc cgtcgggtcc actgccgaga
     1081 tcaccacacc tctttcttcc ggctctacaa acaccagacc catactcacg atcgtcacga
     1141 caccggcggc gatgccgagg accacgagga tcgtattaag cggcttggat ctcttattgc
     1201 ggtaggcgcg cacgacgatg agtaccgcca ctccaataaa tgctaaccac agcaaagcta
     1261 ccaacccctg gaatgcattc gcgaaattca ttattctttc tcccgattga tcaccaaaaa
     1321 tactcttgca gcgattatac ccgttattag gtcgtaataa agcgcccagg gatggttttc
     1381 tgtctggatc attcagcctg tgcacttttc catgcggacg gttgtcgctc agttcgattc
     1441 atgatagcat ccaggcatgc agacgagcac cgggcttgta caggagatcg tattgggggc
     1501 agatggcctg caagcggcct ggatcagctg cccgcccacc agcctcccag cgccaggaca
     1561 atatctaagc gcctggtctc ccatggatcc tctggctccc ctggccgcga ccctgttcgc
     1621 tggaataatc caggaagatc gctttctcgc cttatccccg ctccccgcgt cctggcagcc
     1681 cggcacccgg ctggcgctgc gcggtccgct gggacatggc tttaccctgc cggtgaccgc
     1741 ccgccgggtg gcattggctg ctttcggaca aagctgcgag cggttgatgc ctctcgctca
     1801 cctcgcatta cagatcgaag cctccgtcgc ccttttcaca gatggctggc tgccgccact
     1861 gcctgccgcg ctggaagcca accccctgag cagcctgccg gaagccctca gctgggcaga
     1921 ctatctggcg gttgaagcag accataccac gctgcctcag ctgcggacaa agctcaacgt
     1981 accgccgggc gaacatctcc cttgcccggc gcaggtcttg ttcagggtgg atatgccctg
     2041 tgcgggcctg gctgtctgcg gagcctgctg gacccctgcc cggcggggtc tgctgtatgc
     2101 ctgcagcgat ggaccagtct ttacgctgga tgacctggaa tggtaaaagg agaccctcag
     2161 gtacctcgat gaatatactc gccttttttg ctcaccccga cgatgaaacc atgctggctg
     2221 gcggcacact ggccttgctc gcccacaccg gtgcgaacgt ccattatctg tgcgccaccc
     2281 gcggcgaagg aggcgagacc ggggagcccc cgttgtgcca gcctgagcaa ctgggcgaag
     2341 tgcgcgagga tgagctggcc tgcgcagtcc aggcgctgca cggcgccagc ctggacttcc
     2401 ttggttatgc cgacccccgt gtcggacccg atgaagcttt gttcgcttat accaaccagc
     2461 cgggagaggt cgtccatcat ttgttagcgg aaattcgcga gaacgatatt caagccatcg
     2521 tgacccacgg ctcgaatggt gaatacggac accctgctca caccttgagc taccgggcag
     2581 cccgccaggc tgtagaaagc ctgggagccg cggcgcctct tttgtattgt gcgatggcga
     2641 gttttccgga ccatccccgc ccgcgcttga tgaataaaga tcagtcagcc cacttcgtgc
     2701 tggatattac tccagctctc cctgccaaaa ccagagccgc gctgtgccac cagacgcagc
     2761 atgccctgtt cgtgcgccgc ccttcccagg atgctgggcg gatgctcagc gttccagaag
     2821 tgatcttaac cctggagggc ttgcaccgcg cctttccagc cactgccggt gagccccagg
     2881 acgagctctc agacaggctt aaaccctggc ggcgtactcc ctgaaccacc gaatgaattc
     2941 atatggctaa gtacgatctt gccctgtccc caaccctgat gaatgccgcc ggctcgctcg
     3001 gcttcgcacc gcctggccat gcagccattc aatgggagcg ttttggagct ttcgtgacca
     3061 acccgatcag cctggggcgc cgccaacctg ccaacagcgc cggtcagata tctttttcgg
     3121 gcggatttct cctgcatacc ggctacccca acccgggctt cgaaacaacc ctgcggcggt
     3181 ttcgcgccgc ctggtcgcat gctcctgtgc ctgtgctggt gcatttgatt ccccggacag
     3241 ccgctgagct ggctgcgatg atccccccgc tggagagctg cgataatctg gctggcatgg
     3301 agattgggat tgctcccgat attaccctgg aggaagtggt ggca
//
LOCUS       DATMXK010000355         4179 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_67106, whole genome shotgun sequence.
ACCESSION   DATMXK010000355 DATMXK010000000
VERSION     DATMXK010000355.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4179)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4179)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4179
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_67106"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(105..251)
                     /locus_tag="VN363_06600"
     CDS             complement(105..251)
                     /locus_tag="VN363_06600"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84218.1"
                     /translation="MASHIRVMLEIGPKGKKVVAVAPDWPGLERGAKTAEAAIERLLS
                     YGPR"
     gene            complement(347..496)
                     /locus_tag="VN363_06605"
     CDS             complement(347..496)
                     /locus_tag="VN363_06605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84219.1"
                     /translation="MNDDKLDRLIELQLKSNEMLGQLGLLLTRMAYMILILWIITLVV
                     MVLTA"
     gene            complement(578..>4179)
                     /locus_tag="VN363_06610"
     CDS             complement(578..>4179)
                     /locus_tag="VN363_06610"
                     /inference="COORDINATES: protein motif:HMM:NF013955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="IPT/TIG domain-containing protein"
                     /protein_id="HWQ84220.1"
                     /translation="GLPGDVITGLAAEPGGAVWAIGSGGASRRDPDSGIWTMISTLNS
                     TAIASDPSGRVWLGTTDGLWQYNKVTWTVYRTSMTLLDSNRILGLASDGLRTWVASGG
                     NVQARADFNGPIGFIPPYINSFVPVAGGVSDPVSLYGGGYDIRRTSNNTVSFGNLSDP
                     MTYGAVTYVDAGQINVEVPLLALSGQIWVRANRLNGASAGSFTVIPKIRSVTPTCAAM
                     GGELTIEGSGFNSTNEASYIKIGNGPWRFADAQTPTGLRYRLRPGDGDGTVSVRVGVG
                     GTPVTSTQSVTIDTPVIDQVGIQQGIQGLPLVWAKRTLIMLSMRSQRGLCSSRVDGGK
                     LEFKLKDGSTREDWLSFQPTAAGLTIGPVAPPLGVSNSVNFVMWSQALSRPDFSINDF
                     DGVRLHLFSGPVEVVTYDVPNNSFFFTDLDSRRQHMNVYIYDTSIASNASWDAFYKNA
                     EKGLDHVARAYPQSDLGAYYGRDKWMTWAWRTIGRTGLVDLSGDDTFHRLQGQVEDIR
                     SSANDNGGNYDQAMGVIEASLRTGNTSGKATYDCSNPFGDCDMHSAVAFNTTDNLAHT
                     WMQEAIHTMGWIADSSPNHDGGNKTHSRYNNSVGGTCTPAITFRQALVDQLGYAARVV
                     RLEYGKTPVQFLFADCTTAQMPNSVMSYAPGSQDNNSFLEPLDYFYVFNNIRIFGRTV
                     ASGSGIETGTDEIVPGVIDTLGDQFGLTASPQTHQELRLNGEISGDEPHTVTITMSYL
                     DNPGGDLTEPTPGGQYILSFRDASGANLLDFPFSVGTGHTHGAPEKDFRFGLRVPFPD
                     ATARVAISHDGQELWSSPVSPGAPLVSLTSPVQYQSYNAVDPLPVTWNASDPDGDPLS
                     FILEYSPDDGATWQTIATGLSGSGYDWIPAFSPVGISRLRITATDGFHTAQATSEQFY
                     IQPDKPIAIIQSPADGQVISEGGEIALVGGVVASESSDPVQFDWYYDGNYAGSGQYLY
                     DYLTDIGAHTIGLQVIANGLPSEMAMITFTVIKDADWDGLPNDYEQSYGINPLDRSDS
                     AADPDGDGLSSLVEFQIGTSPVSADSDGDTMNDGDELEQGGNPSDAGSLPPTAATLNV
                     GAQRMGFIFRQGDPNPDPWTIWITNAGAGALAWTASAPAGWLNVSPAAGSAPTALTLS
                     ADPSGLAPGVYTTYLTVSAPGAVGSPRSIPIWIRVYDANGETSYNTYLPVMKK"
BASE COUNT          791 a         1263 c         1271 g          854 t
ORIGIN      
        1 ccccagaagt cgggtgagcc catgcccgga tattgctcga ccacatccac gccggtgatg
       61 ttcggaaact cggcctccat tccggccagc ttcgccaccg tcgcttaacg gggaccatag
      121 gagagtaagc gttcgatagc agcttccgcg gtctttgccc cgcgctcaag accgggccag
      181 tccggcgcta ctgccaccac cttcttgccc ttcggtccga tttctagcat cacccgaata
      241 tggctggcca tagtttctcc tttcgcagtc gattgcactc catagctgcg ttcctacgaa
      301 tgagtacaga ctgtcgggtg gctggtttgg ttttgttggg atgctttcac gccgtcagca
      361 ccatcaccac cagcgtaata atccacagga tcaagatcat atacgccatg cgcgtcaaca
      421 gcaggcccag ctggcccagc atctcattac tctttagttg cagctcgatc aggcggtcca
      481 gcttatcatc attcatggtg cggctcctta aaccagggaa taaagggatg cagggttcat
      541 tgtaacactg ccggaaccct gcatcccttc cccggcttta tttcttcatg accggtaaat
      601 aagtattgta gctggtctcc ccattggcgt cgtacacccg gatccagata gggatcgagc
      661 gcgggctccc caccgcgccg ggggcgctga ccgtgaggta agtggtgtac acgccgggag
      721 ccaatccgct gggatcggcg ctcaaggtga gcgcagtggg ggcgctgccg gcggctgggg
      781 aaacattcag ccaccccgca ggcgcgctgg cggtccaggc cagcgcgcct gctccggcat
      841 tggtgatcca tatcgtccag gggtcagggt tcgggtctcc ctggcggaag ataaagccca
      901 ttctctgggc acccacattc agggtcgcag cggtgggggg caggctgccc gcatcgcttg
      961 gattcccgcc ttgctccagc tcgtcgccat cgttcatggt gtcgccgtcg ctgtctgccg
     1021 agaccggcga agtgccgatc tggaactcca ccaacgagga aagcccgtcg ccatccggat
     1081 cggcggcgct gtccgagcgg tccaggggat tgattccgta gctctgttcg taatcgttcg
     1141 gcaggccatc ccaatcggca tcctttatga ccgtgaacgt gatcatagcc atctcgctgg
     1201 gcaagccgtt ggcgatcacc tgcagcccga tagtatgcgc cccgatatcg gtgaggtagt
     1261 catatagata ctgtcccgaa ccggcgtaat ttccatcgta gtaccagtcg aactgcactg
     1321 gatcgctgct ctcggaggca accacaccgc ccaccagggc gatctcgccg ccctcggaaa
     1381 tcacctgccc gtctgcaggg ctttggatga tggcgatcgg cttgtcgggc tggatataga
     1441 actgctcgga ggtcgcctgg gcggtatgaa agccatctgt agcggtgatg cgcagccggc
     1501 tgatgcccac cggggaaaac gccgggatcc aatcgtaccc ggatccgctc agcccggtgg
     1561 cgatggtttg ccaggtagcg ccgtcgtcgg gcgagtattc gaggataaaa ctgagcgggt
     1621 cgccgtccgg atcggaagca ttccaggtca ccggcagcgg gtcaaccgcg ttatacgact
     1681 ggtactggac cgggctggtc aggctgacca gcggagcgcc ggggctgacc gggctggacc
     1741 acagttcctg accgtcgtgc gagatggcta cccgggcggt ggcatcgggg aaaggcaccc
     1801 gcaagccaaa gcggaaatcc ttctcaggag cgccatgggt atgccccgtt ccgaccgaga
     1861 atgggaaatc caacaggttg gcgccgctgg catccctgaa gctcaggata tattgcccac
     1921 ccggggtggg ctcagtcaga tcgccgcccg gattatccag gtaggacatc gtgatcgtga
     1981 cggtgtgggg ctcatccccg ctgatctcgc cgttgaggcg cagctcctgg tgggtttgag
     2041 gtgaggcagt aagcccaaac tggtcgccga gcgtgtcaat cacgcctgga acgatctcat
     2101 ccgtccccgt ttctatcccg gagccggagg caaccgttcg cccgaagatg cggatattat
     2161 tgaagacata aaagtaatcc agcggctcga ggaaggaatt gttatcttgg ctgccgggag
     2221 cataggacat cacgctgttt ggcatttgcg cggtagtgca gtcggcgaac aggaattgga
     2281 ccggggtttt gccgtactcc aggcgcacca cgcgggcggc gtagcccagc tggtccacca
     2341 acgcctggcg gaaggtgatc gccggcgtgc aggtgccgcc cacggaattg ttgtaccttg
     2401 aatgggtttt attgccccca tcgtggttgg gcgagctgtc tgcgatccag cccatcgtat
     2461 ggattgcttc ctgcatccag gtgtgggcca ggttgtcggt cgtattaaac gccaccgccg
     2521 aatgcatatc acagtcgccg aagggattgg agcaatcgta ggtggccttc cccgatgtgt
     2581 tcccggtcct caaggaggct tcaatcaccc ccattgcctg gtcgtaattg ccgccgttat
     2641 cattggcgct ggaacggata tcttctacct gaccttgcag gcgatggaaa gtgtcgtcgc
     2701 cgctcagatc gaccaatccg gtccgcccga tggttctcca ggcccaggtc atccacttat
     2761 ctctgccgta gtaagctccc aggtccgact gcggataggc tcgcgccaca tggtcgaggc
     2821 ccttttcggc atttttatag aatgcatccc aggaggcatt ggaggcaatg ctggtgtcat
     2881 agatgtaaac attcatgtgc tggcgcctgc tatccagatc ggtgaagaaa aatgagttgt
     2941 tcgggacatc gtaagtcacc acttccaccg gcccgctgaa caggtgcagg cgcacgccat
     3001 cgaaatcgtt gatcgagaaa tccggacggg aaagcgcctg gctccacatc acgaagttga
     3061 cgctgttgct cacacccagg ggaggtgcga ccgggccgat ggtcaggccg gccgcagttg
     3121 gctggaagga aagccagtcc tcgcgggtgc tgccatcttt aagtttgaac tccagcttcc
     3181 caccatcgac gcgcgagctg cacaggccgc gctggctgcg catggagagc atgatcagcg
     3241 tgcgcttggc ccataccagc ggcaatccct ggatcccctg ttggatccct acctggtcga
     3301 ttaccggtgt gtcgatagtg acgctctgag tgctggttac tggcgttccg cccaccccga
     3361 cccgcacgct gaccgtgccg tcgccatcgc cggggcgcaa gcggtagcgc agcccggtgg
     3421 gggtctgggc atcggcgaag cgccacggac cgttgccaat tttgatgtag gaagcctcat
     3481 tggtcgagtt gaagccgctg ccttcgatag tgagctcgcc gcccatcgcc gcgcaggtgg
     3541 gggtgacact gcggatctta ggaatcaccg tgaagctgcc agcgctggcg ccgttcaggc
     3601 ggttggcgcg tacccagatc tgcccgctga gggccagtaa aggcacttcc acattgatct
     3661 gtccggcatc cacgtaggtc accgcgccgt aagtcatcgg atcgctcagg ttgccaaagc
     3721 tgaccgtgtt gttggatgtg cggcggatat catatccacc gccgtagagc gataccggat
     3781 cgctgacacc cccggcgacg ggtacaaagc tgttgatgta tggaggaata aaaccgatcg
     3841 ggccattgaa atcagcccgg gcctgtacgt tacccccgct ggcaacccag gtgcgcaggc
     3901 catcggaagc cagcccaagg atccggttgc tgtccaggag cgtcattgag gtccggtaca
     3961 ccgtccaggt gactttgtta tactgccaca gtccatccgt ggtgcccaac cagacgcgcc
     4021 ccgatggatc ggaggcgatc gcagtcgagt tcagggtgct gatcatcgtc cagatgccgc
     4081 tgtccgggtc acgccgggag gcgcccccgg agccgattgc ccacaccgcg ccgccgggct
     4141 cggctgccag accggtgatc acatctccag gcagcccat
//
LOCUS       DATMXK010000356         4321 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_108624, whole genome shotgun sequence.
ACCESSION   DATMXK010000356 DATMXK010000000
VERSION     DATMXK010000356.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4321)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4321)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4321
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_108624"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..694)
                     /gene="ccsA"
                     /locus_tag="VN363_06615"
     CDS             complement(<1..694)
                     /gene="ccsA"
                     /locus_tag="VN363_06615"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560338.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c biogenesis protein CcsA"
                     /protein_id="HWQ84221.1"
                     /translation="MESRPRLLTIIDSIAVVLLVIATGLVFFYAPTEAVMGNAQKVFY
                     FHVASAWVGMLSFIVAAVAGIAYLRTSARKWDHLGLASVEIGIAFIFIATVTGSIWAR
                     PIWNTWWTWDPRLTTVTIMELIYLAYMMLRSGIEDPDRRARFGAVYAIIGALSVPLTY
                     FSARLFRTIHPVVIGSGDPSAQGTFDMTPRMLQTFMFSLLTFSFIFISLLWHRVRLGK
                     LADKVEQLKLKVM"
     gene            complement(755..1486)
                     /locus_tag="VN363_06620"
     CDS             complement(755..1486)
                     /locus_tag="VN363_06620"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560339.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015232 - heme transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA];
                     GO_process: GO:0017004 - cytochrome complex assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme exporter protein CcmB"
                     /protein_id="HWQ84222.1"
                     /translation="MKDQSLSLPKTRPETRRRNGVAFFSAVRAVVWKDLAAELRSREM
                     LSAMLVFSLLVILIFNFALELDAATRSTVTSGVLWVTFAFAGTLGLNRSMAMEKDRGC
                     LDGLLLAPVDRSAIYFGKAIGNLIFMFIVELIVLPVYSILYGTNLFNLGLLLVIGLGS
                     IGYVAVGTLLSSMAVQTRTRDILLPILLFPVAIPIFVAAVKASTGYLQALPMTEIWPY
                     LNLLIVYDVIFTSVAYMIFDFVVEE"
     gene            complement(1489..2196)
                     /gene="ccmA"
                     /locus_tag="VN363_06625"
     CDS             complement(1489..2196)
                     /gene="ccmA"
                     /locus_tag="VN363_06625"
                     /EC_number="7.6.2.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560340.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0015439 - ABC-type heme transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015886 - heme transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heme ABC exporter ATP-binding protein CcmA"
                     /protein_id="HWQ84223.1"
                     /translation="MIIVHKLVKRFGLKTVLRGLDFQVEPGEFVALLGPNGAGKTTFL
                     RILASLSRPGMGEVRIAGFALPQQAAAVRQRLGVVSHLPLLYGDLTAEENLRFYGRMY
                     NVPNLDHRILEVLELVGLSARRRDLVRTYSRGMQQRLAIGRAVLHDPEVMLFDEPHTG
                     LDQDASAMLDGVLREVAVRGRTVVMTSHDLARVGDLATRFDVLSRGQIAASVSRADLD
                     PNELLGFYKAAIQEAVL"
     gene            complement(2219..4117)
                     /locus_tag="VN363_06630"
     CDS             complement(2219..4117)
                     /locus_tag="VN363_06630"
                     /inference="COORDINATES: protein motif:HMM:NF012264.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWQ84224.1"
                     /translation="MKKLRALLFFPVVILLSACSFSLAEDITPPPNYQPTELPSTQIA
                     PAGALFPVAAPDPQAGQAIYAEKCAPCHGETGLGDGSQAAQLSNPVPALGSAELARQA
                     SPAEWYQVVTQGRMDRFMPPFTSLTDSERWDVVAYALSLSAGETALARGEELYQANCA
                     VCHGDTGLGDGPNAESLTVSPTNFTDQKGMAAKSTSELAQVIRTGAAPAMPAFAQLSE
                     EDVWALSDYIRSLSFAEPGQLVASPPDSTAGAAATPLVGATPLAAVPVEEGVVSGQVT
                     NASGGAVPESASVVLRGFDEMQEVITETAALASDGSFRFENIEMPAGRVFLATVDYKG
                     TTYGSDISEVEAEQREINLPISIYDSTTDSSILVTDRLHLFFEFVDEQTLRIIQLYII
                     SNPTSMTLVSEDPTTPTVNFALPEGAANLEIQDGVIGERFVQTEDGFGDTTAIRPGSG
                     SYQVLYAYELPYKNKLELVQPLTIPTSAVVILVPEGVINIKGERVEDAGVRDVEGTQY
                     RMYNLVGLNAGADLRLTVSGKMAGSGLSLITGDSSSLLIGVGVFGVVLLAAGGWLFMR
                     DRARTDEEAEQDAAAVENAPAVVESRETLMDAILALDDLYQEGHLPKDAYQERRAALK
                     ARLRDLEG"
     gene            complement(4142..>4321)
                     /locus_tag="VN363_06635"
     CDS             complement(4142..>4321)
                     /locus_tag="VN363_06635"
                     /inference="COORDINATES: protein motif:HMM:NF024645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc ribbon domain-containing protein"
                     /protein_id="HWQ84225.1"
                     /translation="APQPVDSQAELAADDSIEVALAQRRRQRQEKSAGFCPQCGGPVQ
                     KSDRFCPKCGTVLSN"
BASE COUNT         1044 a         1277 c         1148 g          852 t
ORIGIN      
        1 ccatcacctt caatttcaat tgttcaactt tgtcagccag cttacccagg cgtacccggt
       61 gccacagcaa gcttataaag ataaacgaaa atgtcaacag actgaacata aaggtctgta
      121 acatcctggg agtcatgtca aaggtgccct gggcactggg gtcgccggag ccaattacaa
      181 ccgggtggat cgtccggaat aagcgcgccg agaagtaagt cagcgggaca ctgagggcgc
      241 caataatggc gtaaactgca ccgaagcgcg cccgccggtc agggtcttcg attccggagc
      301 gcagcatcat gtaagccaga tagatcagct ccataatcgt gacggtcgtc aaacgcggat
      361 cccaggtcca ccaggtgttc cagatcgggc gagcccagat tgagccggtt acagtggcga
      421 tgaaaatgaa tgcaatcccg atctcaaccg aggccaggcc gaggtggtcc cacttcctgg
      481 ccgaagtacg cagataagcg atcccggcaa cggcagccac aatgaaggac agcatcccaa
      541 cccaggccga agcgacatgg aagtaaaaca ctttttgggc gtttcccatc actgcttcgg
      601 taggcgcata aaagaaaacc agcccggtgg cgatcaccag caggaccaca gcaatcgaat
      661 caataattgt gagtaaacgg ggtcttgatt ccatacaatc ggctctcctc tgaaaattta
      721 tcccttatga gtccagcgat cttactggtc cagactattc ttcaacaacg aaatcaaaaa
      781 tcatatacgc aacgctggta aagataacat catacacgat cagcagattc aaatacggcc
      841 agatttcggt cattggcaag gcctgcaggt atccggtact ggcctttacc gccgccacaa
      901 agatggggat cgctaccgga aacagcaaaa taggcagcag aatatcccgt gtgcgcgtct
      961 ggaccgccat gctcgaaagc aaggtcccaa ccgcgacata accaattgaa cccaatccga
     1021 ttacgagcag aagacccagg ttgaacaggt tggtgccata caggatgctg taaaccggga
     1081 ggacgatgag ctcaacgatg aacatgaaga tcaggttgcc aatcgccttg ccaaagtaga
     1141 tcgcggaccg gtcaactggc gccaacagca acccatccaa gcagccgcga tccttttcca
     1201 tcgccatgga gcggtttaat cccagggtgc ccgcaaacgc aaaggttacc cacagcacac
     1261 ctgacgttac cgtggagcgg gtggcggcat ccagctccag tgcaaaattg aaaatcaaga
     1321 ttaccagcag tgaaaacacc agcatcgccg agagcatttc ccggctgcgc agttcagcag
     1381 ccagatcctt ccacacaacc gctcttaccg cgctgaaaaa cgcgacccca tttcgccggc
     1441 gcgtctctgg ccttgttttc ggcaaggata acgactggtc tttcatacct aaagcacagc
     1501 ctcctgaatt gccgccttat agaagcccaa taattcattt ggatccaggt cggcacggct
     1561 gactgaggct gcgatctggc cgcgcgagag cacgtcaaac cgggtagcca gatcgccgac
     1621 gcgggccaga tcatgggagg tcatcaccac ggttcgcccg cggacagcca cctcacgcag
     1681 cacgccatcc agcattgccg aagcgtcctg atccaggccg gtatgcggct catcgaaaag
     1741 catcacttcc gggtcgtgca gcactgctct gccaatcgcc aggcgctgct gcatccccct
     1801 cgaatacgtc cggacaagat cgcgccgccg ggcagacagc ccgaccaatt cgaggacttc
     1861 caggatgcgg tgatccagat ttggaacatt atacatccgc ccgtagaagc gcaggttttc
     1921 ttcagcggtt aaatcgccat ataggagagg taaatgtgag actactccca ggcgctggcg
     1981 cacagcggca gcttgctggg gcaaggcaaa tccggcaatg cgcacttctc ccatgccagg
     2041 ccgcgagagg gaagccagga tgcgcaaaaa ggtggtctta cccgctccat tgggccccaa
     2101 caacgccaca aattcgcctg gctcaacctg gaaatccagg ccgcgtagga cggtcttaag
     2161 cccaaagcgt ttgacaagtt tgtggacgat gatcatgcga ttctcattcc taccggtttt
     2221 agccttcgag gtccctcaag cgcgccttaa gtgcagcccg gcgttcctga taagcgtcct
     2281 ttggcaaatg gccttcctgg tagagatcat ccagggctaa gatggcatcc atcaaagtct
     2341 cccgagattc aaccactgct ggtgcatttt cgacagcagc tgcatcctgc tcagcttctt
     2401 catctgtcct cgcccgatcg cgcatgaata accacccccc ggctgccagc aagacgactc
     2461 caaacacacc taccccaatc aacaggcttg aagagtcacc cgttattaag gataacccgc
     2521 tccctgccat cttgccagac accgtcaaac gcagatcagc tccggcgttc agaccgacca
     2581 ggttgtacat gcgatactgg gtgccttcta catcgcgcac gccagcatct tcaacccgtt
     2641 ctcccttgat attaatcaca ccttcaggga ccaggatcac caccgcgctg gtcgggatcg
     2701 tgagcggttg gaccagctct agcttattct tgtaaggcag ctcataggca tacagcacct
     2761 ggtagctgcc cgagccaggg cggatcgcgg tcgtatcgcc aaaaccgtcc tccgtttgca
     2821 caaagcgctc gccgattaca ccatcctgga tttccaggtt ggcagctcct tccggcagag
     2881 cgaaattaac tgtcggtgtc gtggggtctt cagacaccag ggtcatactg gtgggattgg
     2941 aaataatata cagctggatg atcctcaggg tctgctcgtc tacaaactca aagaataaat
     3001 gcaggcggtc tgtgaccagg atcgacgaat cggtcgtgct gtcgtagata ctgatgggga
     3061 ggtttatttc acgctgttca gcttcgacct ccgaaatatc agagccatag gtagtgccct
     3121 tgtaatccac agttgccagg aacacccgcc cggcaggcat ttctatattt tcaaatcgaa
     3181 aactgccgtc tgaagcgagc gcagcagtct ctgtgattac ctcttgcatc tcgtcgaatc
     3241 cccgcaggac cacgctggct gactcgggga ccgctccacc agacgcattt gtgacctgtc
     3301 cagagacgac accctcttca accggcaccg ctgccagcgg ggtcgcacca acgagcgggg
     3361 ttgcagcggc tccagcggtg gagtctgggg gagacgccac gagctggcct ggctcagcaa
     3421 atgatagcga gcgaatatag tcgctcaacg cccatacatc ctcttcgctg agctgcgcaa
     3481 aagccggcat ggcaggcgca gctccggtgc ggatcacctg agccagctcg gaagtcgatt
     3541 ttgcagccat ccctttctgg tcggtgaaat tcgtcgggga aacggtcaag ctttccgcat
     3601 ttgggccatc ccccaggcca gtatccccat ggcagacagc acagttagcc tgataaagct
     3661 cttcgccccg cgccagagca gtctctccag cacttaagct caaggcataa gctacgacat
     3721 cccagcgctc agaatcagtc aggctggtaa agggaggcat aaagcggtcc atccgtccct
     3781 gcgtaaccac ctggtaccat tctgccggtg acgcctggcg ggcgagctct gctgaaccca
     3841 acgccggcac cgggttggac agctgggctg cctgtgatcc atcccccaga ccggtctcgc
     3901 cgtggcaggg agcgcatttt tcggcataga tggcctgccc cgcctggggg tccggtgcag
     3961 cgaccgggaa caaagcacct gccggtgcaa tctgcgtgct gggaagttca gtcggctggt
     4021 aattcggagg tggggtgata tcttctgcca gcgaaaatga acaggcagac agcaggatga
     4081 cgaccgggaa aaagagcaag gcgcgaagtt ttttcataca gattcagttc aaattggccg
     4141 gttaatttga gagcacggtg ccgcacttgg ggcagaagcg atctgatttt tgaaccggac
     4201 cgccgcactg tgggcaaaaa ccggctgatt tctcctggcg ctggcggcgg cgttgggcca
     4261 gggcgacttc aatactgtcg tccgcggcca actcggcttg cgaatcaacc ggctgcgggg
     4321 c
//
LOCUS       DATMXK010000357         3432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_46143, whole genome shotgun sequence.
ACCESSION   DATMXK010000357 DATMXK010000000
VERSION     DATMXK010000357.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_46143"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1342)
                     /locus_tag="VN363_06640"
     CDS             complement(<1..1342)
                     /locus_tag="VN363_06640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84226.1"
                     /translation="MINPRTLRQTQFLLAGTLLLQVYAFYRLFRSPYTSGILWISRRW
                     LVIFGAASLLTVSLILIFLLTFTPARQKLITGYIWLEKQLSRMGKWNLVVFCIIFVLF
                     SFSLLSSTGNYFNNQYVRIHLFWLSALLGTSLLQAAGWQKPWLVRLAATALAGAFIYR
                     VAAYIPEITNYPLSLGWSEASRYYYASLYFARELYGEMAPLTVLHPSRYLMQSIPFFV
                     PNSPIWLHRLWQVILWIAVTLPTAWLVARRVTTNRSWQRYIVTAWCFLFLLMGPVYYH
                     LSFITLLVLWGFDRRKLWLTILVVFAASLWAGISRINWLPMPAMLAAALYILEEPAGT
                     RKLWKYLAPVAGWGIFGTLTAYGAQTAYSRLSGNPADQFTSSFSSDLLWYRLLPNPTY
                     PTGILLAAAVVILPLIGIIYLRSRQSAGRLHWIRWLVLSGMLLILLAGGLVVSVK"
     gene            complement(1351..2319)
                     /locus_tag="VN363_06645"
     CDS             complement(1351..2319)
                     /locus_tag="VN363_06645"
                     /inference="COORDINATES: protein motif:HMM:NF015651.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lysylphosphatidylglycerol synthase transmembrane
                     domain-containing protein"
                     /protein_id="HWQ84227.1"
                     /translation="MTQAPESQGAKPSRKMLLLRTAGSLLALALLIYLLSKQGWDEIL
                     VALRQIPVSHLLLALGLMFVSRLAVVGRWHVLLTSSGFNISYWQSLRITFAGLFASNF
                     LPTTIGGDVVRLAGAVQNRQDPAVAAASLIVDRLVGMAGMLLAVPFSLAAVSRADIFN
                     NLPSSSRSIQILSLGLWAPIRKIYLKALGIVKRLFDAAGIWLRQPRSLLISLGFTWIN
                     MLCLFGILDLLLGDIDKPVSYWLIGGLYALVYFFTLIPISINGYGLQEVSMTFLFTQV
                     AGVPLSSALTAAVLFRTLMMVTSLPGAVFVPGMLTRTPESTDISEA"
     gene            complement(2316..3359)
                     /locus_tag="VN363_06650"
     CDS             complement(2316..3359)
                     /locus_tag="VN363_06650"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWQ84228.1"
                     /translation="MDLSIVVPVYNEEESLPHLHKAFEDAFNQAEYSWEVIYVNDGSA
                     DGSLQILEMLAAQDPEHVRAVALRRNFGQTAAIAAGIDHSIGDVIVLIDADLQNDPSD
                     IPMMMEKIDQGYDVVSGWRIKRQDTFFTRKLPSQLANGLISRVTGVHLHDYGCTLKAY
                     RREVLTGFRLYGEMHRFIPAYASTVGARMIELPVKHHARKYGHTKYGLTRTVKVILDL
                     FTVKFLMSYANKPIYLFGGAGLTIILVSTIMLIGLIIRRIFWGVEVLSSPFFVMSIMF
                     AIMGFQSILMGLIAELQVRTYHESQHKPTYTLLKYLPGNQKTSPIPGDLDQIKSPVEL
                     VPDSQTEIAGISG"
BASE COUNT          966 a          860 c          900 g          706 t
ORIGIN      
        1 tttttacgct caccaccaat ccaccagcca gaaggatcaa aagcatccct gaaagaacca
       61 gccagcgaat ccaatgcagc ctgccggccg attgcctgga tcggagataa atgattccga
      121 taagaggcaa aatcacgact gccgcagcga gcaaaatgcc agtgggataa gttggattag
      181 gaagcaaacg gtaccagagt aaatctgaag aaaagctgct ggtgaattga tccgcagggt
      241 tcccagaaag gcgagaataa gcagtttggg caccataagc tgtcagggtt ccgaatatgc
      301 cccagccggc gaccggggca aggtacttcc agagcttacg agttccagca ggctcttcaa
      361 gaatatacaa cgcagcagcc agcattgcgg gcattggcag ccagtttatc cggctgatcc
      421 cggcccaaag cgaggctgca aaaacgacca ggatagtcag ccatagcttt cggcggtcga
      481 acccccacaa aaccagcaag gtgatgaagg ataaatggta ataaactgga cccatcaata
      541 aaaacaaaaa acaccaggca gtgacaatgt accgctgcca ggaccggttt gtggttaccc
      601 ggcgtgcgac gagccaggct gtgggtagag tgaccgcgat ccacagaatg acttgccaga
      661 ggcggtgcag ccagatcgga gaattcggaa cgaagaaagg aatcgactgc attaaataac
      721 ggctgggatg taaaacagta agtggtgcca tttcgccgta cagttcgcgt gcaaaataga
      781 gtgaggcata ataatagcga ctggcttctg accaacctaa agaaagtgga taattggtga
      841 tctcagggat ataggcagcc actcgataga taaaggcacc tgccagagct gtggctgcca
      901 gacggaccag ccagggcttc tgccagccag cagcctgcag caagctagtt cccaacaacg
      961 cgctcagcca gaacaggtga attcgcacat actggttatt gaaataattt ccggtggaac
     1021 tcaacaggct gaaggagaaa agcacgaaaa tgatacaaaa gactaccaga ttccattttc
     1081 ccatacggga aagttgtttt tctagccaga tgtacccagt gattaacttc tgccttgctg
     1141 gtgtaaaagt caaaaggaat atcaggatga ggctgactgt aagcaggctg gctgctccaa
     1201 agatgaccaa ccagcgcctg gaaatccata aaatcccgct ggtataaggg gatcggaaga
     1261 gtcgataaaa cgcataaacc tgcagcagga gtgtacctgc cagaaggaac tgggtctggc
     1321 gcaaagtgcg gggattaatc atgacgccga tcaagcttct gagatgtcgg tgctttcagg
     1381 ggtcctggtg agcatgcctg ggacaaatac cgcacctggc aggctggtta ccatcatcaa
     1441 tgtgcgaaag aggactgccg cggtcaaggc gctggacaaa ggaacaccag ccacctgggt
     1501 aaatagaaag gtcatggata cttcttgtaa accataacca ttaatggaga tggggatcag
     1561 ggtgaagaaa tagaccaggg catacagccc gccaattaac caataactga ctggtttatc
     1621 gatatcgcct aagagcaggt ctagaatacc gaacaaacac agcatattta tccaggtgaa
     1681 accgagtgaa atcagcagac tgcgcggctg gcgcagccag attcctgccg cgtcgaacag
     1741 gcgcttgacg atccccaggg ctttaagata gatttttcga attggtgccc ataacccgag
     1801 actcaggatc tgaatcgagc gactgctgga tgggagattg ttgaaaatat ctgctcttga
     1861 cacggccgcc aggctaaaag gtacagccaa cagcatcccg gccatgccga ccaggcggtc
     1921 gacaattaac gaagctgccg caacggcagg gtcttgccgg ttttggactg cgcctgccag
     1981 ccttacaacg tctccaccga tggtggtagg taaaaaattg gaagcgaaaa gacctgcaaa
     2041 tgtgattcgc aggctctgcc aataggagat attaaaacca gacgaagtta acaggacgtg
     2101 ccagcggcct acaaccgcca gccgggacac aaacatcaat ccaagcgcca gcagcagatg
     2161 ggataccggg atctggcgta aggcaaccag gatttcatcc catccctgct tgctgagtag
     2221 ataaatgagc agcgccaggg caagcaagga tcccgccgtg cgcaagagca gcattttgcg
     2281 ggatggcttc gctccctgag attcgggagc ctgggtcatc cagaaatacc agcaatttca
     2341 gtttgcgaat cgggcaccaa ttcaacaggc gatttgattt gatcaagatc cccaggaatg
     2401 ggtgaggttt tctggttgcc cggcagatat ttgagcagtg tgtaggtagg tttatgttgc
     2461 gattcgtgat aggtgcgcac ctgtaattcc gcaatcaacc ccatcaagat tgattgaaag
     2521 cccataatcg caaacatgat gctcattacg aagaacggcg aggagaggac ttcaacgccc
     2581 cagaatatgc gccggataat caatccaatc agcatgatcg tgctgaccag aataatggtc
     2641 aaacctgccc cgccgaagag ataaatcggc ttgttggcat aactcatcaa gaatttaacg
     2701 gtgaacaggt ccagaattac tttaaccgtc cgggtcaacc catatttggt gtgtccatat
     2761 ttacgggcat gatgtttcac cggtaactcg atcatgcgcg ctcctaccgt gctggcatac
     2821 gccggtataa agcgatgcat ctcgccatac aggcggaagc cagtcaggac ttcgcgacgg
     2881 taggctttca gtgtgcagcc ataatcgtgc aaatgtaccc cggtgacccg agagatcagt
     2941 ccattggcaa gctgagacgg gagctttcgg gtgaaaaatg tatcctgtct tttgatccgc
     3001 cacccgctga ccacatcgta gccctggtcg attttctcca tcatcatcgg aatgtccgat
     3061 ggatcgtttt gtaaatcggc atcgatcagc acaatgacgt caccgatcga gtggtcgatg
     3121 ccggctgcga tcgcggctgt ttgaccaaaa ttgcggcgca gtgccaccgc ccgcacatgt
     3181 tccgggtcct gtgctgccag catctcaaga atctgcaggc tgccatccgc gctgccgtca
     3241 ttcacataga tcacttccca gctgtactca gcctggttga acgcatcctc aaaagcttta
     3301 tgtaaatgtg ggaggctttc ttcttcgtta tacaccggga ctacgatcga taggtccaca
     3361 aaaaactcct tagtgggtaa gctcctgggc aacactcgcc aggtcagcat ccaccatttg
     3421 acggattaat ac
//
LOCUS       DATMXK010000358         3519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_153100, whole genome shotgun sequence.
ACCESSION   DATMXK010000358 DATMXK010000000
VERSION     DATMXK010000358.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3519
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153100"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..273
                     /locus_tag="VN363_06655"
     CDS             <1..273
                     /locus_tag="VN363_06655"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_004050440.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HWQ84229.1"
                     /translation="AVESAGIILVRSNPKDIVKIIQLSRASYRKMMQNLGWATGYNII
                     ALPLAAGLLAPWGIILSPAVGAILMSVSTIVVAINAQLLRGVKLAD"
     gene            complement(363..539)
                     /locus_tag="VN363_06660"
     CDS             complement(363..539)
                     /locus_tag="VN363_06660"
                     /inference="COORDINATES: protein motif:HMM:NF016811.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YHS domain-containing protein"
                     /protein_id="HWQ84230.1"
                     /translation="MVIDPVCGMEIDPETAAGSSEYMGKTYYFCSLGCKQDFDKDPEH
                     YIQAEGHAGHTHPH"
     gene            complement(586..843)
                     /locus_tag="VN363_06665"
     CDS             complement(586..843)
                     /locus_tag="VN363_06665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84231.1"
                     /translation="MKLLFTVLLVTLLTSVWITGPALADHGDQMGNCPPVFELHHFTD
                     HSGEHMHQHIGLDQDLNSDGWICMQMLPNDLHLHVDNNLPF"
     gene            complement(927..2114)
                     /locus_tag="VN363_06670"
     CDS             complement(927..2114)
                     /locus_tag="VN363_06670"
                     /EC_number="2.5.1.48"
                     /inference="COORDINATES: protein motif:HMM:NF005871.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0019346 - transsulfuration [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cystathionine gamma-synthase"
                     /protein_id="HWQ84232.1"
                     /translation="MKFETECIHAGQSPDPATGAVMTPIYQTATYLQDGLGKPRGYEY
                     SRTGNPTRAALEACLASLESGEFGLAFASGLAATDTVLRLLQPGDHILAGNDVYGGTY
                     RLFEQVLRPYGLEFTYVDTTDLEQVAAGLRPDTRLVWLETPTNPLLRVSDISAVSRLV
                     KNAPSIPLVVVDNTFATPFLQRPLELGADLVVHSTTKYLGGHSDVIGGAVVGRDLAHR
                     DRLAFLQNAVGAVPGPMDCFLILRGIKTLPIRVTRHSENAAALADYLADHPAVERMIY
                     PFHAWHPQAAIARRQMAMGGGMISFTLKGGAEAAQRVAESTHIFALAESLGGVESLIE
                     VPSVMTHASTASSGLAIDPGLVRLSVGIEHIADLQADLEYALDQIETSPVLRDKFRLR
                     TTN"
     gene            complement(2138..>3519)
                     /locus_tag="VN363_06675"
     CDS             complement(2138..>3519)
                     /locus_tag="VN363_06675"
                     /EC_number="4.2.1.22"
                     /inference="COORDINATES: protein motif:HMM:TIGR01137.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004122 - cystathionine beta-synthase
                     activity [Evidence IEA];
                     GO_process: GO:0019344 - cysteine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="cystathionine beta-synthase"
                     /protein_id="HWQ84233.1"
                     /translation="TVGNTPLVHLNRLGRDLACALYAKVESFNPGGSVKDRIAVNIIA
                     EAERSGRLKPGGTVVESTSGNTGVGLAIVCAKKGYRTVFVMPDKMSQEKIQLLRAFGA
                     KVVITPTAVAPEDPRSYYNVAKRIVSETPNAILANQYHNPENPRSHYLTTGPEIWEQT
                     QGKVTDVVIGMGTGGTISGIGRYLKEQNQRVRIIGVDPVGSLLLETWQQGRLPPGGEA
                     LPYKVEGIGEDFLPSTLDLSVIDEVIQIGERESFVWARRLVKEEGIFCGGSSGTALAA
                     AMHYASGLGPDHLVVVLLPDSGSRYLSKFYDDKWMKENGFLETEWSQVQLMDVLAAKR
                     RSLPVSASAELISVQTSDRITDVIALLKAHDISQVPVLHPDGSLAGLVAEVDLLKFML
                     ENDPRNSVSATVAAIAAPAEAVFPSTELLEAALPAILEGYVALVTQDRRPVGILTKID
                     LLDFLAQKI"
BASE COUNT          813 a          980 c          923 g          803 t
ORIGIN      
        1 gcagtcgaat ccgcggggat catcctggta cgcagtaacc ctaaggatat cgtcaagatc
       61 attcagctca gccgggcctc ataccggaag atgatgcaga atttggggtg ggcaactggt
      121 tacaacatca tcgcccttcc cctggcagcc ggactattgg cgccgtgggg gatcatcctc
      181 tcccctgcgg ttggggcgat cttgatgtcg gtcagcacga tcgtggtagc aatcaatgcc
      241 cagctgctgc gtggcgtgaa gctggccgat taagagatca ttgaattaaa aacaaaaccc
      301 tcccatatat tgttgatgat caatgggagg gtttgtcttg ctaccgcttg tacagtatca
      361 gatcaatgcg ggtgggtatg cccggcgtgc ccttcggctt gaatgtagtg ctcaggatct
      421 ttgtcaaaat cctgcttgca gccgagtgag caaaaatagt atgtcttgcc catatactcc
      481 gaggagccgg cggctgtttc tggatcaatt tccataccac atactggatc aattaccatc
      541 gttttctctc cttgtaatca atacttatcc tgccaatgtc ttaccttaga aaggcaggtt
      601 gttatcgacg tgcagatgta gatcattggg aagcatctgc atgcaaatcc agccatcact
      661 atttagatcc tgatcgagac cgatgtgctg atgcatatgc tcaccagaat gatccgtgaa
      721 gtggtggagc tcaaatactg gcggacagtt ccccatttga tcaccgtggt cagccaacgc
      781 cggacctgtt atccaaacgg atgtcagcaa agttactaac aggacggtaa ataatagctt
      841 catttccatt cctccttcgg aacgctaaca taatgtaaat tcagcggaaa gaagttcctt
      901 ctcgccgatt ggggatgtat ctgactctaa ttcgttgttc tcagtcgaaa cttatctctg
      961 agcaccgggg atgtctcaat ctggtccaac gcatattcaa gatctgcctg gagatctgcg
     1021 atatgctcga ttcccaccga taagcgcacc agacccgggt cgattgccag acctgagctg
     1081 gcggtcgagg cgtgggtcat tacagatggc acttcgatca acgactctac gccacccagc
     1141 gactcggcca gggcgaagat gtgtgtgctt tcggcaactc gctgggcggc ctccgctcca
     1201 cctttcaatg tgaacgatat cattcctcca cccatcgcca tctgccgcct ggcaattgca
     1261 gcctgggggt gccaagcatg aaaagggtag atcatacgct caacagcagg gtgatctgca
     1321 agataatctg ccagagcggc agcgttttcc gaatgccggg taacgcggat cggcagggtt
     1381 ttgatcccac gcaggatcag aaaacaatcc attggacccg ggaccgcgcc aaccgcgttc
     1441 tgtaggaaag ccagccggtc ccggtgcgcc agatcccggc ctacaacagc cccaccgatc
     1501 acatcggaat gcccgcctag gtatttggtt gttgaatgaa ccaccaggtc agcgcccaac
     1561 tccaatggac gctgcaggaa gggtgtggca aaggtattat cgactacaac cagcgggatc
     1621 gagggagcat ttttaaccaa ccgggataca gctgagatat cgctgactcg cagtagaggg
     1681 ttggttggcg tttcaagcca gaccaggcgt gtatccggcc gcagtccagc cgccacctgt
     1741 tcaagatcgg tggtatccac ataggtgaat tcgagaccat agggtcgcag aacttgctca
     1801 aaaagcctgt acgtaccgcc ataaacatca ttcccagcca ggatatggtc acccggctgt
     1861 aacaaacgca gcacggtatc cgtagcggcc agaccggaag cgaacgccag cccgaattca
     1921 ccactttcca aacttgccag gcaggcttcg agtgcagcgc gggtgggatt cccggtgcgc
     1981 gaatattcat agccgcgcgg cttacccaac ccatcttgta gataagtcgc ggtttggtaa
     2041 attggggtca tcactgcccc ggttgccggg tctggcgatt ggccggcatg gatacattcg
     2101 gtttcgaatt tcacgctgag caactcctgt ggttcaatca aatcttctgg gcgaggaagt
     2161 ccagcaggtc gattttcgtc aggatcccca ccgggcgccg gtcctgggtc accagggcga
     2221 catacccttc caggatcgcg ggcaaggcag cctcaagtag ttccgtggat gggaagacag
     2281 cttcggcagg ggctgctatc gcggcgactg tcgccgatac cgaatttctg ggatcgtttt
     2341 ccagcataaa tttaagcaga tcaacctcag caaccagccc ggccaggctg ccatcgggat
     2401 gaagcactgg cacctgagag atatcgtggg ctttgagcaa ggcaatgaca tcagtgatcc
     2461 ggtcgctggt ttgaacggaa atgagctctg ccgacgcact taccggcagg gaccggcgct
     2521 tggctgccag cacatccatc aattggacct ggctccattc cgtctccaga aagccattct
     2581 ccttcatcca tttgtcgtca tagaatttag ataaatagcg cgagcctgaa tcgggcaaca
     2641 ggaccactac cagatgatct ggacccaggc cgctggcata atgcatggcg gctgccaggg
     2701 ctgtacccga cgacccaccg cagaatattc cttcttcctt caccagcctg cgcgcccaga
     2761 cgaatgattc gcgttcgccg atctggatca cctcatcgat cacagataaa tcgagcgtgg
     2821 aaggcaagaa gtcctcgcca atcccttcaa ctttataagg cagcgcttca ccgcccggag
     2881 gaagtctgcc ctgctgccag gtttccagga gcaatgagcc aaccgggtcc acaccaataa
     2941 tccgtacgcg ctgattttgc tccttgaggt atcgcccaat cccgctgatc gttccaccag
     3001 tgcccatccc aatcacaaca tcagtcacct tgccctgggt ctgttcccag atctccggac
     3061 cagtcgtgag gtaatggctg cgcgggtttt cagggttgtg gtattggttg gccaaaatgg
     3121 cattcggggt ttccgacaca attcgtttag caacgttata gtatgagcgt ggatcctcag
     3181 gagcgacggc cgtaggcgtg atgacgactt tggctccaaa cgcacgcagc agctggattt
     3241 tttcctggct catcttgtcc ggcatgacaa aaaccgtgcg atagcctttt ttggcgcaga
     3301 caatcgccag ccccaccccg gtgttaccgg aagtcgattc tacgacggta ccgccaggct
     3361 tgagccgccc actgcgttcg gcttctgcga tgatgttgac tgcgatccgg tcttttacag
     3421 agccacccgg attgaaggat tcaactttgg catacaaggc acaggctaaa tctctcccca
     3481 ggcgattcaa atgaaccaat ggagtatttc cgacggttt
//
LOCUS       DATMXK010000359         2091 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_125899, whole genome shotgun sequence.
ACCESSION   DATMXK010000359 DATMXK010000000
VERSION     DATMXK010000359.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2091)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2091)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2091
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_125899"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1193)
                     /locus_tag="VN363_06680"
     CDS             complement(<1..1193)
                     /locus_tag="VN363_06680"
                     /inference="COORDINATES: protein motif:HMM:NF027582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016853 - isomerase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tagaturonate epimerase family protein"
                     /protein_id="HWQ84234.1"
                     /translation="MNTSPGFTIFPGSIHTANAAPHSARSLTCFMARQEADGDRVLGV
                     HLPPGSPPAPFNSLLHVSGSDHFFALTSENAAALRALFPWLNPVPLEPDVPVSQFRPS
                     FGFGDRLGVATPGHIQALRALNSPSTIAPIFAQQSVRENARTGRTPQQVLDDAMWGVF
                     QEGWRLPWGADADHLKQPSDIPPFITAGYTFFTIDPGEFVDSAADTDPSEVLEAKIAA
                     QPWDEIQPVNESSPASLTAAALVEEFQSFCAAHNLACERDLLDLAALRAQAKYGRAVI
                     HIVRMYRAVLALKPDGFDFETSVDETETPTSVLEHYYIASCLKRFGVRFTSLAPRLPG
                     KFNKGVDYIGDLAALEAEIKNHAAVMRHIGGYKLSLHSGSDKFSVYPILARHAGLAIH
                     VKTAGT"
     gene            complement(1190..>2091)
                     /locus_tag="VN363_06685"
     CDS             complement(1190..>2091)
                     /locus_tag="VN363_06685"
                     /EC_number="5.3.1.12"
                     /inference="COORDINATES: protein motif:HMM:NF014652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008880 - glucuronate isomerase activity
                     [Evidence IEA];
                     GO_process: GO:0006064 - glucuronate catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glucuronate isomerase"
                     /protein_id="HWQ84235.1"
                     /translation="LEAHQALRTSGWSGDIRPCFRPDGVINLDMQGWRANLEKLAEAT
                     GLEINTYPRFIQALESQRAFFKSLGDTATDHAALLPRTELLTAVEAETIFQRALLGAS
                     QPGDARRFTGHMLVEMARMSIEDGLVMQIHSGSLRNHNPQVFDRFGLDKGADIPIAGE
                     FTRNLRPLLERFGNDPRLRLILFTLDESTYARELAPLAGHYPSVLLGPPWWFHDSPNG
                     MRRYFDQIIETAGLYNTAGFNDDTRAFASIPARHQVWRRVSANWLAGQVLHGLITETD
                     AADMLADLASGLARKAYRLETRP"
BASE COUNT          455 a          552 c          719 g          365 t
ORIGIN      
        1 gtcccggcgg tcttaacgtg gattgcaaga ccggcgtggc gagccaggat cggataaacg
       61 ctgaacttgt cggaaccgga gtgcagactg agcttataac caccgatatg gcgcatcact
      121 gcagcatgat ttttgatctc ggcttctaaa gcagccagat cgccgatgta atcgacacct
      181 ttattgaact tgccgggaag acggggagcc agactggtaa aacgaacccc gaagcgtttg
      241 aggcaactgg caatatagta gtgttcgagc acactggtcg gcgtctcggt ctcgtccaca
      301 gaggtctcaa agtcgaagcc gtctggcttg agggccagga cggcgcggta catacggaca
      361 atatggatca cagcccggcc atacttggcc tgggcgcgca gagcagccag atcgagcagg
      421 tcgcgttcac acgccaggtt gtgggcggcg cagaacgact gaaattcttc aaccagagca
      481 gcggcggtca gcgatgcagg gctgctctca ttcacaggtt gaatttcatc ccagggctgg
      541 gcggcgattt tagcctccag cacctccgaa gggtccgtat cagcggccga gtccacgaac
      601 tctccgggat cgatggtgaa aaaggtgtag ccggcggtga taaaaggcgg gatatcggag
      661 ggctgcttga ggtggtcggc gtcagctccc cagggcaggc gccagccttc ctggaagaca
      721 ccccacatgg catcgtcgag cacttgctga ggggtgcgcc cggtgcgtgc attttcgcgc
      781 accgactgct gggcgaagat aggggcgatg gtggaggggg aattgagggc gcgcagggcc
      841 tggatgtgcc ccggagtggc gacacccagc cggtcgccaa agccgaagga aggccgaaat
      901 tgggagacgg ggacatccgg ctccaatgga accgggttca gccaggggaa taaggcgcgc
      961 aaggcggctg cattctccga agtgagggcg aaaaagtgat cgctgccgct gacgtgaagg
     1021 agggaattaa atggagccgg ggggctgccg ggaggtaagt ggactcccag aacgcgatca
     1081 ccatcagctt cctgacgcgc cataaagcag gtcagggagc gggccgaatg tggggcggcg
     1141 ttggcggtgt ggatcgagcc ggggaagatg gtgaaaccgg gagatgtgtt catgggcgag
     1201 tctccaaacg ataggcttta cgggccagac cagaagccag gtcggccagc atgtcggcgg
     1261 cgtcggtctc ggtgatcagg ccatgcagca cctgcccggc cagccagttg gcgctgaccc
     1321 gccgccagac ctggtggcgg gcagggatcg aggcgaaggc acgggtatca tcgttaaaac
     1381 cagccgtatt gtagagtcca gctgtttcaa tgatctgatc gaagtagcga cgcatgccgt
     1441 tcgggctgtc gtggaaccac cagggcggac caagcagcac ggaggggtaa tgaccagcca
     1501 ggggggccag ctcgcgggca taggtgcttt catccaaagt aaacagaatc aggcgcaggc
     1561 gagggtcatt gccaaaacgc tcaagcagcg ggcgcaggtt ccgggtgaat tccccggcga
     1621 tgggtatatc ggcgccttta tccaggccga agcggtcgaa aacctgaggg ttgtggttgc
     1681 gcaatgagcc ggaatgaatc tgcatgacca gcccatcttc gatgctcatg cgcgccatct
     1741 caaccagcat atgcccggta aagcgccggg catcgccagg ctgggatgcg ccaaggagag
     1801 cgcgttggaa aatagtttca gcctcaacgg cggtgagaag ctcggtacgg gggaggaggg
     1861 cggcatggtc ggtggctgtg tcgcccagcg atttgaagaa ggcacgctgg gattcgagtg
     1921 cttgaatgaa tcggggataa gtgttgatct ccagaccggt cgcctcggcc agcttctcca
     1981 ggttggctcg ccagccctgc atatccaggt tgatcacgcc atcggggcgg aagcaggggc
     2041 ggatatcccc gctccaaccg gaggtgcgca gcgcctgatg ggcttccaac c
//
LOCUS       DATMXK010000360         4519 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_26138, whole genome shotgun sequence.
ACCESSION   DATMXK010000360 DATMXK010000000
VERSION     DATMXK010000360.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4519)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4519)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4519
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_26138"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            83..1726
                     /locus_tag="VN363_06690"
     CDS             83..1726
                     /locus_tag="VN363_06690"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWQ84236.1"
                     /translation="MKRLLQGIAKKKVLIPLVAAGLLLIVLVGGSLRARGSSAELVYA
                     TVELGNLTQTIEASGMVRAKQTADLAWKISGTVESVAAAAGQQVEAGQVLTSLEKSSL
                     SAGVILAQARLVNAERELEELKTSRLGQAEAMNALEEARQAWEDAQNPLVAQADAQNA
                     VAAAQQELDKANRLLAVTASVPSADAISQANANLLLAENVYNQTLENIERIQKRLHKP
                     ESAYMFFESRELYRGILDSLEQKEIRDRRAYEKAQEKYNQLLEPVDPLDLAVAEANVV
                     GAMAALDNARRQWERIQDGYSAGDLAVLEAQLRDAEREWERLKDGPDAGDLTAAEARV
                     AAAQAVLTQVEIKAPFSGVITRVDTKVGDQVRAGTSAMRLDDLTHLYVDVHVSEIDIS
                     QVRVGQTAVLTFDSIPVQKDNRALGENTGSATAQVAYTGTVVAVARVGTEVDETVQYD
                     VVIEIDQPDGLIHPGVSADATIVVNEIQDVVKIPNEAVYYTSTTRVVFVARAGQPEAV
                     VVDLEAVTTEYSQLVAGDVQPGDRIVMDPPEWLTVDLEG"
     gene            1774..3327
                     /locus_tag="VN363_06695"
     CDS             1774..3327
                     /locus_tag="VN363_06695"
                     /inference="COORDINATES: protein motif:HMM:TIGR01730.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005215 - transporter activity [Evidence
                     IEA];
                     GO_process: GO:0006810 - transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWQ84237.1"
                     /translation="MKKWIWIGLGVLVIAAVIYFVGPNQAQQQAAQASNYQTETAQFG
                     SLTATVGATGVVHANQTAFLVWQTTGTVAEVQVGVGDPVEKDEVLASLAQTSLSQQVI
                     LAQADLVTAQRALDDLMNSQIQSAQAQQAVEQAERALEDAQNPALTQARAQEAIATAQ
                     KAVENAERVYRYSLSPASQSMIDEAQARVTIAKDSLDRAAERFEPYAGRPEDNLTRAQ
                     LQSQLSAAQQQYDAAVRNLNSMQGTASATDQAVAEANLISAQAQFAQAQRDYERVKDG
                     ANLADIALLEAQLEDARREWERVKDGPDPDDLRAAEARRNAAQATLDLARLAAPFSGT
                     ITVVDSKPGDQVTPGTSGFRLDDLSHLLIDVRVSEVDINRIRVGQDVTLGFDAIQDKV
                     YNGRVVEVDLVGSIDQGVVDFNVSVELTDADADVKTGMTAAVNIVVEQLDNVLLVPNR
                     AVRISEGNRVVYILQDGNLEEIEINLGASSESSSQVLEGDLQSGDLIVLNPPLVFNNN
                     GGPPPFMVR"
     gene            3331..4011
                     /locus_tag="VN363_06700"
     CDS             3331..4011
                     /locus_tag="VN363_06700"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84238.1"
                     /translation="MSEHVIQATSLTKVYKMGEMEVHALRGLSTTIDRGEAVAIMGPS
                     GSGKSTLMNIIGCLDRPTSGEYILDGEDVSKLKDDQLAVIRNRKVGFVFQSFNLLSRT
                     SALANVELPMRYAGMGRGRRDLAKTALESVGLADRIHHHPNELSGGQQQRVAIARALV
                     NNPAIILADEPTGNLDSASGKEIMQLLLSLNVERNTTLIIVTHDPEVAAHTQRVIHIR
                     DGVVEGQT"
     gene            4008..>4519
                     /locus_tag="VN363_06705"
     CDS             4008..>4519
                     /locus_tag="VN363_06705"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84239.1"
                     /translation="MKFWQSLIDALESLAANKMRSILTVLGIVIGVAAVIAMISIGRG
                     AENTITGSIQGIGTNLIFVLRGGSDEVRNPKPITMGDADAMSDPFQAPSVELVAPMLQ
                     GGASYSFGGETSNSPVEAVTPAYGPVRNNTVAEGEFITEEHILGRASVVVLGPDLAEK
                     LFGRSQGVTG"
BASE COUNT         1041 a         1124 c         1431 g          923 t
ORIGIN      
        1 agggcaatgc gcctgaaggg caatgcgcct gaagggctaa tcgccctgag tgggcttacc
       61 cagagcatgt tgaaggatta ttgtgaagcg cttgttgcaa ggtatagcaa agaaaaaagt
      121 tctcattccc ctggtcgcgg ctggattgtt attgatcgtc ctggttgggg gatcacttcg
      181 ggcgcggggt tcaagcgcgg aattagtgta tgcaacggtc gagctgggca atctgaccca
      241 aaccattgaa gcatcgggaa tggtgcgtgc caaacagact gctgatttgg cctggaagat
      301 ttctggcaca gtcgaaagtg tggctgctgc agccggccag caggtagaag ccgggcaggt
      361 gttgaccagt cttgagaaaa gctcactttc tgccggggtg atcctggcac aggcgcgcct
      421 ggttaatgcc gagcgagaat tggaagagct caagacgtcg cgcctgggcc aggctgaagc
      481 gatgaatgcc ctggaagagg cgcgacaggc ctgggaagat gctcaaaatc cacttgtggc
      541 gcaggctgac gcccagaatg cagttgcagc agctcagcaa gagctggaca aagctaatcg
      601 cttattggcg gtcacggctt ctgttccatc agcagatgca atcagccagg caaacgcaaa
      661 cctcctgctg gctgaaaatg tttataacca gactctcgag aacatagagc ggatccagaa
      721 gcggctgcat aaacctgaaa gtgcatatat gtttttcgaa agtcgcgagc tgtaccgggg
      781 gatcctggac agtttggagc agaaagagat ccgcgaccgg cgagcttacg aaaaagccca
      841 ggaaaaatat aaccagctcc tggagccggt tgacccgctg gacctggcag tggcggaagc
      901 caatgtggtt ggggccatgg cagcgctgga taatgcccgg cgtcagtggg aacgcatcca
      961 ggatggctat tcagctggag atctggctgt gctggaagct cagctgcggg acgcagaacg
     1021 cgaatgggag cgcctgaaag atggtccgga tgcgggcgat ctcaccgcgg cggaagctcg
     1081 cgtagctgcg gcgcaagcag tcttgactca ggtagaaatc aaagcgccgt tcagcggcgt
     1141 gattacgcga gtggatacga aggtgggcga ccaggtacgc gccgggacca gcgccatgcg
     1201 tctggacgat ctgacccacc tgtacgtgga cgtccatgtc tcggagatcg atatcagtca
     1261 ggtccgggtc ggtcagacgg cagtattgac ctttgattcg atcccggttc aaaaagataa
     1321 tcgtgcgttg ggtgaaaata ctggcagcgc caccgcccag gtggcgtata ccggaacggt
     1381 tgttgctgtt gcccgggtgg gaaccgaggt cgatgaaact gtccagtatg atgtagtgat
     1441 cgaaattgat cagccagatg gtctgatcca cccgggagtc agcgcggatg ccaccatcgt
     1501 ggtgaatgaa atccaagatg tggtgaaaat cccgaatgaa gcggtgtact ataccagcac
     1561 cacgcgggtt gtgtttgtgg caagggcagg gcagcctgaa gctgttgtag ttgatctgga
     1621 ggctgtaact acggagtaca gccagttggt ggcaggggat gttcagccag gcgaccggat
     1681 tgtgatggac ccaccggagt ggctcacggt ggacctggaa ggttaaattg atcgaaaata
     1741 taatggtttt ttttgaccaa tgggaagggt tctatgaaaa aatggatctg gattggcttg
     1801 ggcgtgcttg tgatcgcagc agtgatttat ttcgtggggc caaatcaagc ccagcagcag
     1861 gctgcccagg caagtaatta tcaaaccgaa actgcccaat ttggcagcct gacagcaact
     1921 gtcggggcga ccggggttgt acacgccaat caaacggcat tcctggtctg gcaaactacg
     1981 ggaacagttg cagaggtcca ggtgggggta ggtgatccgg ttgagaagga tgaggtgctg
     2041 gcttctctgg cacaaacttc gctttcgcag caggtgatcc tggcccaggc agatttagtt
     2101 acggctcaac gcgctctgga cgacctgatg aactcgcaga tccaatccgc gcaggcacaa
     2161 caagccgtcg agcaggccga gcgggctctg gaagatgcgc agaatccagc attaacccag
     2221 gctcgcgctc aggaagccat cgcgactgcc caaaaagcag tcgaaaatgc tgagcgagtc
     2281 taccgttaca gcctgtcgcc tgccagccag agcatgatcg acgaagctca ggcacgcgta
     2341 accatcgcta aggacagttt agaccgggcg gctgagcgtt tcgagccata tgccggaagg
     2401 ccggaggata acctgacgcg tgcccaatta cagagccagc tctctgctgc tcagcagcag
     2461 tatgatgctg ccgtgcgaaa cctgaatagt atgcagggga ctgccagcgc caccgaccag
     2521 gctgtggcag aagcgaacct gatctcggcc caggctcagt ttgcgcaggc tcaacgggat
     2581 tatgagcggg tcaaagatgg cgcgaacctg gcggatatag cccttctgga agctcagctg
     2641 gaggatgctc gccgggagtg ggagcgagtg aaggatggtc cggatccaga tgacctccgg
     2701 gctgctgaag cccggcggaa tgcagcccag gcaaccctgg atctggcgcg cctggcagcc
     2761 ccattcagcg gaacgatcac tgtggtggac agtaaacccg gtgaccaggt cacccctggc
     2821 acatctggat ttcgcctgga tgacctgtct cacctgttga tcgacgtccg ggtttcagag
     2881 gtggatatca atcgcatccg ggtcgggcag gacgtcaccc tgggtttcga cgccatccag
     2941 gataaagttt ataacggccg ggttgtcgaa gtcgatctgg tgggcagcat tgatcaaggt
     3001 gtggtggatt tcaatgtgtc tgttgagctg acagacgccg atgcggacgt caaaaccggg
     3061 atgaccgcgg cagtcaatat cgtggtcgag cagttagata atgtcctcct ggttccaaac
     3121 cgggcagtgc gcatctcgga aggcaaccgg gtagtgtata ttctgcagga tggcaacctg
     3181 gaggagattg agattaacct gggagcctct tccgaatctt ccagccaggt gctggagggt
     3241 gatttgcagt caggcgatct gatcgtgctc aatccaccgc tggtgtttaa caataacggt
     3301 ggcccgccgc catttatggt gaggtagcgc atgagtgagc atgtcattca ggcaacgagc
     3361 ctgaccaaag tatataaaat gggcgaaatg gaagtgcacg cactgcgcgg cttatccacg
     3421 actatcgacc gcggcgaagc agtcgcgatc atgggcccat ctggctcagg gaaatccacc
     3481 ttaatgaata tcatcggctg cctggaccgg ccgacctcgg gagagtatat cctggatggg
     3541 gaggatgtct caaaactcaa ggacgatcag ctggcagtga tccgcaatcg caaagtgggt
     3601 tttgtatttc agagcttcaa tttattgtca cggaccagcg ctctggcgaa tgttgagctg
     3661 cccatgcgct acgcgggcat gggtcgcggc cggcgagatc tggcaaagac ggccctggag
     3721 tctgtcggac tggctgaccg gatccatcac caccccaatg aactgtccgg tgggcagcag
     3781 cagcgggtgg cgattgcccg ggcgctggtc aataacccgg cgattatcct ggcggatgag
     3841 ccgaccggaa acctggattc cgcttccggc aaggaaatta tgcagctttt gctttcatta
     3901 aatgtcgagc gcaatacgac tttgattatc gtgacccatg acccggaagt cgccgctcac
     3961 acgcagcggg tgatccatat ccgggatggg gttgtggagg ggcaaacatg aagttctggc
     4021 agtcgcttat tgatgccctg gaaagcctgg cagcgaataa aatgcgttca atccttactg
     4081 tgttggggat tgtgatcggt gtagcagccg taattgccat gatctcgatc ggtcgcggcg
     4141 cagagaatac gatcactggc tcgatccagg ggattgggac caacctgatc tttgtccttc
     4201 ggggcggctc ggatgaggtg cgcaatccga agccaatcac gatgggagat gccgatgcga
     4261 tgagcgatcc tttccaggcg ccatcggtgg agctggttgc gcccatgtta caaggcgggg
     4321 cctcctattc ttttggcggg gagacatcca actcaccggt tgaagccgtt accccggcat
     4381 acggcccggt gcgaaacaac acggttgcgg aaggcgagtt tataactgag gaacacatcc
     4441 tgggccgggc atcggtggtc gtgctggggc cagatctggc ggaaaagctt ttcggaagat
     4501 cgcagggggt gaccggcga
//
LOCUS       DATMXK010000361         2189 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_126878, whole genome shotgun sequence.
ACCESSION   DATMXK010000361 DATMXK010000000
VERSION     DATMXK010000361.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2189)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2189)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2189
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_126878"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2017
                     /locus_tag="VN363_06710"
     CDS             <1..2017
                     /locus_tag="VN363_06710"
                     /inference="COORDINATES: protein motif:HMM:NF015380.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="clostripain-related cysteine peptidase"
                     /protein_id="HWQ84240.1"
                     /translation="DADDKVLEQDIYIDLNEAEKVGSTENVKIVAQTDRFSGGFTGDG
                     DWTSTKRFLVTQDNDLKTVSSQQITDLGEVNMASGDSLVDFVAWVVQNYPADKYALIL
                     SDHGMGWPGGWSDPTSTEGGDPRIPLSSALGDELYLMELDEALTRVREQAGIDQFELI
                     GMDACLMSHLEVYSALQPHARFAVASQETEPSLGWAYSSFLSELAANPNMDGGTLGNF
                     IVESYIVDDQRIVDDQARAEFLRPSSPFGGSFSAAQITNQLTRDITITTVDLAALPVL
                     IDSVNDFAFTLQNVDQSAVAQSRSYAQSFTNIFGSQVPPSYIDLGHFVQLVARATGDS
                     QISQLAQQVMDSLSQTVIAEKHGSNKSGATGISIYFPNSTLYSSPITGAQSYVQVSNR
                     FARETIWDDFLAYHYIGRSFQPAQDTQVIPEASDRVTPPGAEGGIILSPITASSSIAA
                     PGSPILLSTEVTGKNIGFIKLFVGLHDPNANSIYVADTDYLESAQSQELNGVYYPVWD
                     EDGNFNLEFEWEPVVFAINDGSNSAIALFTPESYGKAVEDAVYTVEALYTFADGSGQR
                     RARLYFRDGMLRQVFGFTGEQATGAPREITPEAGDQITIIEQWMDLDENGRVTGTVEE
                     NGETLTFGNQMFTWETLDAPVGNYVVGFIAEDLDGNEYEVYTNVSVQ"
BASE COUNT          506 a          555 c          593 g          535 t
ORIGIN      
        1 ggatgctgat gacaaggtcc ttgagcagga tatttatatt gatttgaacg aagctgaaaa
       61 ggttggctca accgaaaatg tgaagattgt cgcccagacg gatcgcttta gcggtggttt
      121 tacgggggat ggtgattgga cctcaacgaa gcgcttcctg gtgactcagg ataatgactt
      181 gaagacggtt agctcacagc aaattaccga cctgggtgaa gtgaatatgg cttccggtga
      241 tagtctggtt gattttgttg cctgggtagt ccagaattat cccgcagata aatacgcctt
      301 gatcctatct gatcatggaa tgggctggcc tggtggctgg agcgatccca catccactga
      361 aggaggcgac ccaagaatcc cgctgagcag tgccttaggc gatgagctgt acctgatgga
      421 gctcgatgag gctctgaccc gggtccggga acaggctggt atcgatcagt ttgagctgat
      481 tggtatggat gcatgtttaa tgagccacct ggaagtctat tctgcgcttc aacctcacgc
      541 tcgcttcgcg gttgcatctc aggaaaccga gccttcctta ggctgggcct acagcagctt
      601 tttatcggaa ttagctgcta atcccaacat ggatggcggt acactgggca attttattgt
      661 cgagagctat attgttgatg atcagcgcat cgtcgatgac caggcacggg cagaattctt
      721 acgtcccagc tccccattcg gcggcagctt ttccgctgcg caaataacca atcaattaac
      781 ccgcgatatc acgatcacca cggttgattt ggctgctctg cccgtcctga tcgacagcgt
      841 aaacgatttc gcctttactc tccagaatgt cgatcaaagc gcagttgccc aatctcgcag
      901 ctatgcgcag tcatttacca atatatttgg cagccaggta ccaccctcat atatcgacct
      961 tgggcatttt gtgcagctgg tcgcccgggc aactggtgat agccagattt cacagctggc
     1021 ccagcaggta atggattccc tcagccagac tgtgatcgct gaaaaacatg gctcaaataa
     1081 atccggcgca accgggatct ccatctattt ccccaactca accctctaca gctccccgat
     1141 cactggcgcg caatcctatg tgcaggtgtc aaatcgtttt gcccgcgaaa ccatctggga
     1201 tgatttcctg gcataccact atatcgggcg ctctttccag cccgcccagg atacacaggt
     1261 aatcccggag gccagtgacc gcgtcacgcc acctggtgca gaaggtggaa ttattttatc
     1321 gccaatcacg gcctccagct ctattgctgc gcctggttcg cccatcctgc tcagcaccga
     1381 ggtaacgggg aaaaatattg gtttcatcaa gctcttcgtt gggttgcatg acccgaatgc
     1441 caattcgatc tatgtcgcgg acacggatta tctggagagc gctcaaagcc aggagctgaa
     1501 cggtgtgtat tatcctgtct gggatgaaga tgggaacttt aacctggaat ttgaatggga
     1561 acccgtcgtg tttgcaatca acgatggcag caattctgca atcgcactgt tcactcccga
     1621 gagttatggg aaggctgtcg aagatgctgt ttataccgtc gaggctctgt acacattcgc
     1681 cgatggcagc ggtcaacgcc gggcgcggct gtatttccgg gatggcatgc tccgccaggt
     1741 ctttggattc actggagaac aagctacggg tgcgccgcgc gagatcactc ctgaagccgg
     1801 cgatcaaatt acgatcatcg aacaatggat ggatctggat gagaatggtc gtgttacggg
     1861 gacggtagaa gaaaatggtg agaccctcac cttcggaaac cagatgttta cctgggaaac
     1921 cctggatgcc ccggttggca attatgttgt cggctttatc gcagaagacc tggatgggaa
     1981 tgagtacgaa gtgtacacga atgtgagtgt gcagtaagac tctgcgcggg ggaagagttg
     2041 atcctgaacg acgagtctat cgatgaacgt ctgaggcgca ccacagactg gtttgacagc
     2101 tgcggagcgc ctcggtcgac tgcttgcatg cttgtgcatg ggtccaccct tacgcgccat
     2161 tcctggcagc cgcagattga cttcttaag
//
LOCUS       DATMXK010000362         2303 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_104175, whole genome shotgun sequence.
ACCESSION   DATMXK010000362 DATMXK010000000
VERSION     DATMXK010000362.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2303)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2303)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2303
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_104175"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1234)
                     /locus_tag="VN363_06715"
     CDS             complement(<1..1234)
                     /locus_tag="VN363_06715"
                     /inference="COORDINATES: protein motif:HMM:NF012361.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWQ84241.1"
                     /translation="MTRSSQSWPEYRPQVIEPKWQASWEQAGLYVADETSQLPKYYCL
                     DFFPYPSGEGLHVGHCRNYIPTDVMSRYRRMQGFNVLHPMGWDAFGEPSEQYAILHNV
                     HPRIVTDQNTANYRRQLSLLGTSYDWTREIDSSRPEYYRWTQWFFLKMFRRGLAYRDH
                     SWQWWCPGCQTTLSSHEVTGGVCWRGHTGVIRKKIPAWYFRITDYADELLSGLDTIDW
                     PDPIKSMQRNWIGRSEGCEVIFKTPDGLQVPVFTTRPDTLYGVTFFVLAPEHELVAQI
                     TGPAQRPAVEAYQASAAAQSEVERMREDREKTGVFTGGFVVNPLNQEAVPVWVADYVL
                     PGYGSGAVMGVPAHDSRDFEFAQKYALPVRTVIQSLELADDQPAETAFTGPGKLVDSG
                     PFTGLTSQDAIRAITRELG"
     gene            complement(1586..1658)
                     /locus_tag="VN363_06720"
     tRNA            complement(1586..1658)
                     /locus_tag="VN363_06720"
                     /product="tRNA-Ala"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1623..1625),aa:Ala,seq:ggc)
     gene            complement(1714..1783)
                     /locus_tag="VN363_06725"
     tRNA            complement(1714..1783)
                     /locus_tag="VN363_06725"
                     /product="tRNA-OTHER"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
BASE COUNT          507 a          652 c          609 g          535 t
ORIGIN      
        1 gcccaagctc ccgggtaatc gcccggattg cgtcctggct ggtcaggccg gtaaatggac
       61 ccgaatcgac aagcttaccc ggacctgtaa atgctgtttc ggctggctgg tcatcagcca
      121 gttccaggga ctggatgact gtcctgaccg gtaatgcgta tttctgggca aattcaaaat
      181 cgcggctgtc atgagcaggc acacccatca cagcccccga gccataaccc ggcagcacat
      241 agtcggcaac ccataccggc acagcttcct gattaagtgg attaaccaca aagcctcccg
      301 tgaaaacacc ggttttttcg cggtcttcac gcatccgctc cacttcgctc tgagcagcgg
      361 cacttgcctg gtaagcttcc acggccgggc gttgagcagg gcctgttatc tgtgcgacca
      421 gctcatgttc tggcgccagg acgaaaaagg tcaccccata cagggtgtcg ggccgggtgg
      481 taaaaaccgg gacctgaagt ccatcgggtg ttttgaaaat gacctcacaa ccttccgacc
      541 ggccaatcca gttgcgctgc atgcttttga tcggatcggg ccaatcaatc gtatccagcc
      601 cggagagcaa ttcatccgca taatcagtaa tccggaaata ccaggctggt attttcttac
      661 gaatgacacc ggtatggccg cgccagcaaa ccccaccagt cacttcatgg ctggacaggg
      721 tcgtctggca gcccgggcac caccactgcc agctgtgatc gcgatacgcc aggccgcgcc
      781 ggaacatttt caaaaagaac cactgcgtcc agcggtagta ttctgggcga gatgaatcga
      841 tctcgcgcgt ccaatcgtag ctcgtcccca gaagggacag ctggcgccgg tagttggcag
      901 tattctgatc tgttacgatc cggggatgaa cattgtgcag gatcgcgtac tgttcggatg
      961 gctcaccaaa ggcatcccag cccattggat gcaaaacatt aaatccctgc attcgcctgt
     1021 agcgcgacat cacatcggtt ggaatatagt tccggcagtg gcctacatgc aatccctcgc
     1081 ccgatggata ggggaaaaag tcaaggcagt agtatttcgg gagctggctg gtttcatccg
     1141 cgacatacag gcctgcctgt tcccaggatg cctgccactt aggctcaatt acctggggcc
     1201 ggtactctgg ccaggattgg gatgatctgg tcattgtgca cctccgaaat taaacataaa
     1261 atcccctcgc caccagggac gaaggggact ccgcggtacc accctgcttg tccggacgtt
     1321 aaccggaccg ctcaaacgcg gtaacgggcg gatcccgctg ccaattagcg cagacacagg
     1381 ggtctggttc gctggcagag ctggctctac tgagccgggc aggcgagttc agccgcttgt
     1441 gctccacagg caccttgctg ggctcgcacc tcactctccc agctcgctgg tggatggctt
     1501 attactcctg ccgcgttttg tcgataataa ggaacgtcca gtcatccggt attgaatgct
     1561 gaacgttgcc aatgtggact gcgtgtggac ccagtgggat tcgaacccac gaccttctca
     1621 atgccattga gacgcgctcc caactgcgct atgggccctg atgtcgaccg gaacgaaatt
     1681 gtacccggta tccattattc cgtcaataac cagtggacct ggagggattc gaaccctcgg
     1741 tctcttcagt gcgattgaag cccatggctc gtccacagtg tccactacct acataggttg
     1801 aggtggagag cattatacca ctaccagagt ggggataaat gtcctttatt tccgtttatt
     1861 ccatgccgtg ttttcgtggt tgcacacgcg ttacacacga cgtgaagtag ggaaggcaga
     1921 ctgtcgccct gtccaagcgg gagaacacaa gaaggtgggt gggttattcg cttgtattac
     1981 ccccgtgcac ggattttccc tgctttctgg ttgattccct tacattttgt ccagaaatgt
     2041 ccaggaaagg actatgactc ggtttcgatc gttacggttc gttctgtcgc taccagcttt
     2101 gatttggctg gtaccaacgt aggcccacgt ttcaaaaact agtgcgcatc cttgctgatc
     2161 tgactctcaa catggagagt atcggtcaga cggtcaacca aatcattcag cttcaaatag
     2221 attgccaggc ggtctttcgg cttaggccgt cctcgataat cttgccgagc atctcagttt
     2281 tgctcggcaa gattagacct agt
//
LOCUS       DATMXK010000363         2311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161912, whole genome shotgun sequence.
ACCESSION   DATMXK010000363 DATMXK010000000
VERSION     DATMXK010000363.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2311
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161912"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1255
                     /locus_tag="VN363_06730"
     CDS             <1..1255
                     /locus_tag="VN363_06730"
                     /inference="COORDINATES: protein motif:HMM:NF013567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="Gfo/Idh/MocA family oxidoreductase"
                     /protein_id="HWQ84242.1"
                     /translation="RWTEGRNMEAFVDLLAAGKIPVQKLISHRFDVDQAPDAYALITG
                     KRAEPFLGVILRYPERDTSVDQSLPVDSGFQTTSLPVNPVRITNSGTSIQLGVLGAGN
                     FATAVLLPAVQKIPSIQRVVIASASGASAQNAARRFGFSEAVSDEAAVLENPRINTVA
                     ILTRHNLHARQVQAGLNAGKNVFCEKPLALDEVELDEIAQALNQNQGRSLLMVGFNRR
                     FAPLAIRLKEFIAGRSEPVVAQYRVNAGFIPLNHWTHDPRQGGGRIIGEGCHFIDLLT
                     FLVGQPPAAVSAVGLPDAGRYREDNLVISLSFPDGSLGTVTYLANGDKAFPKERLEVF
                     CGGRVAVLDDFRSLEMVSAGRRQTQTSRLRQDKGHQAEWLAFNAAVQAGGAPPIPYVD
                     LFGVSRASIAAQQALRTGQTIRLPG"
     gene            1283..>2311
                     /locus_tag="VN363_06735"
     CDS             1283..>2311
                     /locus_tag="VN363_06735"
                     /inference="COORDINATES: protein motif:HMM:NF028199.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III family protein"
                     /protein_id="HWQ84243.1"
                     /translation="MSTRFRKLSLIFKTLGELGPRQVWHFATYQIGLRSGYYRWRSPP
                     RNPAYIPVYPVHLDKIPLPKVDALLDVLDVPARQDLDTLADELRSGKVRLFGGPPVAL
                     RLSPPGPLQHWTAYESQLLNPDTGEAEDIKWIWEPSRCIWGIQWGRTAYLSGSARDSA
                     VFWEYVGQFWTANPPNMGPNWSSAQEIALRLISLCLAARSFSELQPLRPEESDLLSRI
                     VASHADRLPLTLSYARAQNNNHLLAEAAGLYTAGILLPEHPQADRWRDSGWKWFNRGL
                     QTQIAGDGSYIQNSANYHRLMLQLALWVNLLAASRDEKLPSPTRDKLAASTRWLLELV
                     DPDTGSVPN"
BASE COUNT          468 a          662 c          652 g          529 t
ORIGIN      
        1 acgctggaca gaaggccgta acatggaagc cttcgtggac ctgctggcag cgggtaagat
       61 tcctgttcag aagctgatct ctcatcgctt tgatgtggat caggctccgg atgcatatgc
      121 cctgatcacc ggcaaacgcg ccgaaccctt cctgggagtg atcctgcgtt acccggagcg
      181 tgacacttca gtcgaccaat cattaccggt ggactccggc tttcaaacta cctcgctccc
      241 agtgaatcct gttcgcatta cgaattctgg gacgtccatc caactgggcg tcctgggtgc
      301 tggaaatttc gctacagcag tcctgctgcc cgcggtgcaa aagatccctt cgatccagcg
      361 tgtggtaatc gcctctgcct caggcgccag cgcccaaaac gctgcccggc gcttcggctt
      421 ttctgaggcg gtcagcgatg aagccgcggt gctggagaat cctcggataa atacagtagc
      481 catcctcacc cgccacaact tacatgcccg ccaggtccag gctgggctta atgccgggaa
      541 aaacgttttt tgcgaaaagc cgttagcact ggacgaagtt gaattagatg aaattgctca
      601 agctttaaac cagaatcaag gacgttcatt gttaatggtc ggcttcaacc ggcgttttgc
      661 tccactcgcc atcaggctga aggaatttat tgccggtcgg agcgagcccg tggtagccca
      721 ataccgggtg aatgctggct tcattccctt aaatcactgg actcatgatc ccagacaggg
      781 cggaggccgg attattggcg aaggttgtca cttcattgat ttgcttacct ttcttgtagg
      841 ccagccgcct gctgcggtga gcgcggtagg cctgccggat gccgggcgtt acagggagga
      901 taatctggtc atcagcctga gcttcccgga tggatcattg ggtacagtaa cctacctggc
      961 gaacggcgat aaagccttcc cgaaggagcg gctggaagtc ttttgtgggg gacgggtagc
     1021 agttctggat gatttccgca gcctggagat ggtctctgca ggccggcggc agacccagac
     1081 ctctcgcttg cgccaggata aaggccacca ggcagaatgg ctcgccttca atgccgccgt
     1141 tcaggctggc ggcgcaccac cgatccccta tgtcgatctg tttggggtaa gccgggccag
     1201 cattgctgcc cagcaagcct tgcgtaccgg gcaaacgatc cgcttgcctg gttaagccta
     1261 ttgcaggcta tcgattgcga ttgtgtccac ccgattccgc aaactttcgc tgatctttaa
     1321 gactttgggc gagcttggcc cgcgccaggt ctggcatttc gccacctatc agattggctt
     1381 gcgcagcggc tattatcgct ggcgatcacc tcccagaaat ccggcgtaca ttcctgttta
     1441 tcccgttcac ctggataaaa tccctttgcc aaaagttgat gccctcctgg atgtgctcga
     1501 tgtgcccgcc cggcaggatc tggatacgct tgctgatgag ctgcgttccg gaaaggtccg
     1561 tctgtttggg ggtccgccag ttgctttgcg tctttcgccg ccaggaccac tccagcactg
     1621 gacggcttac gaatcacaac tgctgaaccc ggatacaggt gaagcggaag atatcaaatg
     1681 gatctgggag ccatctcgct gtatttgggg aatccagtgg ggacgtacag cgtacttgtc
     1741 cggcagcgcc cgcgactcgg cagttttctg ggagtatgtg ggccagtttt ggacggccaa
     1801 tccgccgaat atgggtccaa attggagctc tgcccaggaa attgctttgc ggctgatttc
     1861 cttatgcctg gcagcccgta gttttagcga gctgcagccg ctgcgcccgg aggaaagcga
     1921 cctattatca cgcatcgtcg ccagtcatgc cgatcgcctg cccctgacct taagctatgc
     1981 ccgtgcccag aataataatc acttattggc cgaagccgct ggcttgtata ctgctggcat
     2041 cctgctgccg gaacatccgc aagcagatcg ttggcgagac tcggggtgga agtggttcaa
     2101 ccgcggctta cagactcaaa ttgccggcga tgggagttac atccaaaaca gcgccaacta
     2161 ccaccggctg atgctgcagc tggccctatg ggtgaacttg ctggcagcct cccgggatga
     2221 aaaactaccc tcgccaacgc gggataaatt ggctgcctcc acccgctggc tgctggagct
     2281 ggtggacccg gatacaggtt ccgtcccgaa c
//
LOCUS       DATMXK010000364         2111 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12939, whole genome shotgun sequence.
ACCESSION   DATMXK010000364 DATMXK010000000
VERSION     DATMXK010000364.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2111)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2111)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2111
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12939"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..975
                     /locus_tag="VN363_06740"
     CDS             <1..975
                     /locus_tag="VN363_06740"
                     /inference="COORDINATES: protein motif:HMM:NF019552.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heparinase II/III-family protein"
                     /protein_id="HWQ84244.1"
                     /translation="SSYADYRPVLQAASRAFLGKPAFPAGSWDEMSGWLVPDRPGPTN
                     FEPDTVQGTGLLVLRPSNIPTWAYLRAAHFTDRPGHADQLHLDLWWRGWNIAGDAGTI
                     LYNASPPWNNALARTAAHNTLVVAGQDQMTHAGRFLWLDRAQAKVLEYQSVPVNNNIW
                     AVAQHDGYRKMGLIHRRKVGWDGTGWLVEDEVFAQAGQPSNRMISVSMHWLLPDCTWQ
                     LLTETPLSMRLQTPAGWVNMNIETTAGEELEPGQVSMQLARAGQLVFGDGQVEPTSGW
                     RSHTYGYKEPALSFRYGLRGQLPLRLTTRWDFLPIITPHPPMQAALTN"
     gene            982..>2111
                     /locus_tag="VN363_06745"
     CDS             982..>2111
                     /locus_tag="VN363_06745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558609.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ84245.1"
                     /translation="MHILLIHQAFAALDEPGGTRHHELARGLAARGHRVTVIASQVSY
                     LTGSVAEQGRSSSVNEEGITILRTYAARAIHRSFVHRMVGFFSFMVSSFWAGLRIRNA
                     DLIWGTSPPIFQGLTAFILARLKGVPFLFEVRDLWPAFAVQVGVLRNPLLIRLSEGLE
                     RFLYRNADQLMVNSPGFIEHVQTRGARQVALIPNGADPRMFDPAADGCDFRKAHQLGE
                     QFIALYAGAHGMSNDLEVVLEAAQLLRDSPGICFVLLGDGKEKPALEERARALQLPNL
                     HFISSIPKTQMPQALAAADACIAILKPIPLYATVYPNKVFDYMAAGRPVLMAIDGVAR
                     QVVEQAGAGIYCPPGSPLQLAQAVRELADDPLRARQMGARGRR"
BASE COUNT          413 a          592 c          616 g          490 t
ORIGIN      
        1 tcctcctacg cagattaccg cccggtcctg caggctgcca gcagggcttt tctgggaaag
       61 cctgccttcc cggctggtag ttgggatgag atgtctggct ggttggtgcc cgaccggccc
      121 ggaccaacca atttcgagcc agataccgtt caaggcacag gcttgctggt gctgcgccca
      181 tccaatatcc caacgtgggc ttacctgcgc gcagcccatt tcaccgaccg gcccgggcat
      241 gccgaccagc tccacctcga tctctggtgg cgcggatgga atattgccgg ggatgccggg
      301 actattctgt ataatgcttc accgccctgg aataatgctc tggcccggac tgcagcccat
      361 aatacacttg tcgttgcagg gcaagaccag atgacccacg ccggtcgctt cctgtggctg
      421 gatcgggcac aagctaaggt attggaatac cagtctgttc ccgtgaataa taatatctgg
      481 gcagtggcgc agcacgacgg ctatcgaaaa atgggtctta tccaccgtag aaaggtcggt
      541 tgggatggga cgggttggtt ggtggaagat gaagtgtttg cacaggccgg acaaccttcc
      601 aatcgcatga tcagtgtttc aatgcactgg ctgttaccgg attgcacctg gcagcttttg
      661 acggaaaccc cgctgagcat gcgattgcag actccagctg gctgggtgaa catgaacatc
      721 gaaactactg ccggtgaaga attggaaccc ggacaggtca gcatgcagct ggcccgagct
      781 ggacaactgg ttttcgggga tggccaggtt gaacctacct caggctggcg atcccatacc
      841 tacggttaca aagagccagc cttatcattt cgatatgggc tgcgtggtca actgccttta
      901 cgcctgacta cgcggtggga ttttttacca ataatcacac cccatccgcc tatgcaagct
      961 gccttgacga actgactgtt gatgcatatt ctgctcatcc accaggcttt tgctgccctg
     1021 gacgaaccag gagggacccg ccaccacgag ctggcgcgcg gcctggcggc gcgtggccat
     1081 cgcgtcacag tgattgccag tcaggtcagt tacctgactg gatctgtagc tgaacaaggc
     1141 cgctcgtcat cagtgaatga agagggcatt accattttac gcacctatgc ggctcgcgcc
     1201 atccatcgca gttttgtgca ccggatggtc ggcttcttta gcttcatggt ttcttcattt
     1261 tgggctgggc tgcgcatccg gaatgctgac cttatttggg gcacttcacc gcccattttt
     1321 caggggctta ccgcattcat cctggcgcgc ttgaaggggg tcccattttt attcgaggtg
     1381 cgtgatttat ggcctgcctt tgctgtgcag gttggggtgc tacggaatcc gctgctgatt
     1441 cgcctttccg aggggttgga gcgcttcctc tatcggaacg cagaccagct gatggtcaat
     1501 tctccgggct ttattgaaca tgtccaaacc agaggtgccc gccaggtagc tctgatcccc
     1561 aatggagccg atccgcgcat gttcgatccc gccgcagacg gttgcgattt ccgcaaggcg
     1621 caccagctgg gtgaacaatt tattgcattg tatgccgggg cgcatggaat gtccaacgac
     1681 ctggaagtgg tcctggaggc agcccagctc ttgcgagact ccccggggat ctgttttgtt
     1741 ttgctggggg atggcaaaga aaaacctgcc ctggaggaac gggccagagc tttacaactg
     1801 cccaatttgc attttatcag ttcgatcccc aagactcaaa tgccgcaagc attggctgcc
     1861 gcggatgcct gtatcgcaat cttaaaaccg atccctttat atgctaccgt ttatccgaat
     1921 aaggtgtttg attacatggc tgcggggcgc ccggtgctga tggcgatcga tggagttgcc
     1981 cgccaggtag tggagcaagc cggggcgggg atttactgtc cgcccggcag cccgctccag
     2041 ctcgcgcagg ccgtccggga gctggcggat gatccgctcc gcgcccgcca gatgggggcc
     2101 agagggcggc g
//
LOCUS       DATMXK010000365         2302 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_70264, whole genome shotgun sequence.
ACCESSION   DATMXK010000365 DATMXK010000000
VERSION     DATMXK010000365.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2302)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2302)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2302
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_70264"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..523
                     /locus_tag="VN363_06750"
     CDS             <1..523
                     /locus_tag="VN363_06750"
                     /inference="COORDINATES: protein motif:HMM:NF024298.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84246.1"
                     /translation="WDAASWEFRRDYHLKVALDMAFSPSSALFAVTPDRYAIQVWDMV
                     TSKQIHTLYTSFTGAVNCLVFSPSGETLVTGHYDGMIRFWNVENGELIREISTDGVIE
                     ALSFSPQGSVLASGSSYQDFSIRLWDVQSGSLLRTLEGHTRGVDSLEFSPNGQMLASA
                     AYDGTLRLWGIRP"
     gene            546..1709
                     /locus_tag="VN363_06755"
     CDS             546..1709
                     /locus_tag="VN363_06755"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84247.1"
                     /translation="MKPVRLGWLLPVIWVAILISGCQTPSIEEAAIPSPEPSPTVEFT
                     ITPTPPAATALPVLVAATQPSPAPTPQPVYTAITSQNAARLALLKAIEFGPWSQITSL
                     EWAPDGKYLAVSAGNQVFLLDPISWENMDRVDVGASSPGLAASPDSRYLAAASRDGRL
                     TVWEIGGTSSLRFSMVYTLEVHRKGANQAAFSPSGEWLASGGNDALARVRRLEDGEEL
                     AQIIGGSYAVADLAFSPDGEWLAIANSSLIRLRRPDSGVMGLTLQAEQPLFCLEFSPD
                     GRWLAAGDSANQVLLWELQRDAAVQVVGEHSGQPGTVEALVWQIDFNPEGNLLASAGG
                     DGAVQIWELTAGESLAELGGHIGAATSARFSPDGLWLATGDLGGNLRIWGVAE"
     gene            complement(1731..>2302)
                     /locus_tag="VN363_06760"
     CDS             complement(1731..>2302)
                     /locus_tag="VN363_06760"
                     /EC_number="2.8.3.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558633.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008410 - CoA-transferase activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="CoA-transferase"
                     /protein_id="HWQ84248.1"
                     /translation="DPTLVSGALGVCSMYDIFSFYLQRGNVDVGFLGGAQIDRFGNIN
                     ATVIGDYEHPKVRLPGSGGAMEIAAWANRCYILTPHQKRRFPEKVDFRTSAGYLNNKA
                     ERDAAHLRGKGPLAVVTDIGILEPDANGEMILTALHPGKTLVQAQENTGWTLRSAPEI
                     RITTPPSEVELHILRTELDPTGIYLKGSG"
BASE COUNT          449 a          651 c          703 g          499 t
ORIGIN      
        1 atgggatgct gccagctggg agtttcgccg cgattaccat ctcaaggtag ccctcgatat
       61 ggctttctca ccatccagtg ccttatttgc agtcaccccg gatcggtatg ccatccaggt
      121 gtgggatatg gtgaccagca aacaaatcca tactctatat acctccttca ccggggctgt
      181 gaactgcctg gtattttccc ccagcggaga aaccctggtg acaggacact atgatgggat
      241 gatccgcttt tggaacgtcg agaacggcga gctgatccgt gagatcagta ccgatggagt
      301 tatagaggcg ctcagtttta gccctcaggg cagcgtgctg gcctcgggca gcagctacca
      361 ggatttttcc atccggctct gggatgtgca gagtgggtcc ctgctgcgga cactggaagg
      421 tcatacccgc ggcgttgaca gcctggaatt ttctcccaac gggcaaatgc tcgcttcagc
      481 cgcatatgac gggaccttgc gcctgtgggg cattcgcccc tgagcggcag tcgagcagat
      541 aattgatgaa accagtgcgc ttgggctggc tcctgcctgt gatctgggtg gcaatcttaa
      601 tttcaggctg ccaaaccccg tctattgaag aggcagcgat cccttcgccc gagccttcac
      661 ccacggttga atttacgatt actccaacac ctccggctgc caccgctttg ccagtgctgg
      721 tggctgcaac gcagccttca ccggccccta ctccccagcc agtttacacg gcgatcacct
      781 cccagaatgc tgcccgactg gccctcttga aagcgattga gtttggtccc tggtctcaga
      841 tcaccagcct ggaatgggcg ccggacggaa aatatctggc agtttccgca ggcaaccagg
      901 tcttcctgct cgatcccata agctgggaga acatggaccg ggttgacgta ggggcttctt
      961 caccggggct ggcagccagc ccagacagcc gctacctggc tgccgccagc cgggatgggc
     1021 gcctgaccgt atgggagatt ggcgggacca gttcgctgcg gtttagcatg gtttacaccc
     1081 tcgaagtcca ccgcaaaggc gctaaccagg cagctttcag cccttcaggc gaatggctgg
     1141 cctcgggtgg caacgatgcc ctggctcggg tacggcgatt ggaggatggc gaagaactgg
     1201 cgcagatcat tggcggcagt tatgcagtcg ccgatctggc tttcagcccc gatggagagt
     1261 ggctggcaat tgcgaacagc agcctcatcc gcctgcgccg gccggacagc ggcgtgatgg
     1321 ggcttaccct gcaagccgag cagcctttat tttgcctgga attcagcccg gatgggcgct
     1381 ggctcgccgc cggggacagc gctaatcagg tgctcctctg ggagctgcag cgagacgccg
     1441 cagtccaggt ggtgggcgag cattccgggc agcctggtac ggtggaggcg ctggtctggc
     1501 agatcgattt taatccggaa gggaatttgc tggcttcggc aggtggagat ggcgccgtac
     1561 agatctggga actcaccgcc ggcgaatcgc tggcagagct aggcggtcat attggagcag
     1621 ccaccagcgc caggttcagc ccggatgggc tctggctggc gactggtgac ctgggtggaa
     1681 atttacggat ctggggtgta gcagaataga gcccttatct gatgattgag ctatccactt
     1741 cctttgagat aaattccagt tggatccagc tctgtgcgca gaatgtgcag ctcaacttct
     1801 gaaggcggcg ttgtgatccg gatttccgga gcggaccgca gagtccagcc ggtattttct
     1861 tgagcctgga ccagggtttt gccaggatgt aaggcggtta atatcatttc gccgttcgca
     1921 tcgggctcca ggatgccaat atcggtgacc accgccaggg gacccttgcc gcgcaggtga
     1981 gctgcatcgc gctccgcttt gttatttagg tagccagctg atgtgcgaaa gtcgactttt
     2041 tcagggaagc gccgcttctg gtggggggtg aggatataac agcgatttgc ccaggcagcg
     2101 atttccatcg cgccgccaga gcccggcagg cgcactttgg gatgctcgta atcgccgatc
     2161 acggtggcgt tgatattccc aaagcggtcg atttgggctc cacctaaaaa gcccacatcc
     2221 acattgccgc gctgcaagta gaagctaaag atatcgtaca ttgagcagac tcccaacgcg
     2281 ccgctcacca gggtggggtc gc
//
LOCUS       DATMXK010000366         2152 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_5637, whole genome shotgun sequence.
ACCESSION   DATMXK010000366 DATMXK010000000
VERSION     DATMXK010000366.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2152)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2152)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2152
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5637"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..326)
                     /locus_tag="VN363_06765"
     CDS             complement(<1..326)
                     /locus_tag="VN363_06765"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84249.1"
                     /translation="MSKPAILIDYLCDGRLRTEPQTRPDAQADTQRHRQPYAHLLAGE
                     LLAWMERSPRFTAFVESYRNKIRKKLRLIRQPEAALDLRSELEAAYRLLADRRLELAY
                     EPYASE"
     gene            622..840
                     /locus_tag="VN363_06770"
     CDS             622..840
                     /locus_tag="VN363_06770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84250.1"
                     /translation="MWGAAAGQRQRRLVRMRCRWSRADYAMVQVHEWPSLEALTQHVA
                     FEQEMEWPRYFDQEHILGVKIPLGAAVV"
     gene            870..1775
                     /locus_tag="VN363_06775"
     CDS             870..1775
                     /locus_tag="VN363_06775"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012517.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="LLM class flavin-dependent oxidoreductase"
                     /protein_id="HWQ84251.1"
                     /translation="MDYRRPIQFGYFPTPAAASYPELLRLAQLCDALGMDLIGIQDHP
                     YQSRFMDTWTLLSALAAQTARIRFFPDVINLPLRPPAVLAKAAASLDIISGGRIELGL
                     GAGAFWEAIAAMGGPVRTPGEAVAALEEAIQVIRLMWSGQRGLRFDGQHYRLKGVHSG
                     PVPAHPIGIWIGAYGPRMLALTGRLGDGWVPTSTYAPPDKLPEMHRRIDSAALSAGRD
                     PGMIQRVYNLMGQIQDRPGGEPFIGPVGQWVAELTRLVLEFGMDTFIIGFPEPPGDQI
                     ERFMAEIAPRVRENVAGERSVSRAS"
BASE COUNT          383 a          662 c          684 g          423 t
ORIGIN      
        1 ttctcactgg cgtacggctc gtaggcgagc tccaggcggc ggtcggccag caggcggtag
       61 gcggcttcca gctcgctgcg caggtccagg gcggcttctg gctggcggat caggcgcagc
      121 ttcttgcgga tcttgttccg gtagctctcg acgaaggcgg tgaagcgcgg ggagcgctcc
      181 atccacgcca gcagctcacc cgccagaagg tgcgcatacg gctgccggtg ccgctgtgta
      241 tccgcctgtg catccggccg agtctgcggc tcggtccgca ggcgaccatc gcacagatag
      301 tcgatcaaaa tggctggctt actcatggca ggattatttt atccccaaaa tttgcggctt
      361 taagcctgaa atcgggatat aatttatcaa cttaagggga accggcaggg aaatcgggct
      421 gcacacatcc gaagcagccg gcacagcgtg ggcagccggg tcattccagg ctgccttgaa
      481 ggcggatcga tccgggaggt ggaccttggc tggacaggtg tgtaaggtgt ttatcctgcg
      541 gcgcttcacc gaagcgggct gcgctttgac ggaggaaagc cgcgccgcca agcgggccga
      601 gctggctgag atccgcaggg attgtggggg gcagctgctg gtcagcgcca gcgccggctg
      661 gtgcgcatgc gctgccgctg gtcgcgcgca gactatgcca tggtccaggt gcacgagtgg
      721 cccagcctgg aagccctgac tcagcatgtg gcgtttgagc aggaaatgga atggccgcgc
      781 tacttcgacc aggagcatat cctgggagtc aaaatccccc tgggcgcggc ggtggtgtag
      841 attgcgcagc cagcggaaaa tgacctgaaa tggattaccg gcggcccatt caattcggat
      901 attttcccac cccggcggcg gccagctatc cagaactgct gcggctggcg cagctgtgcg
      961 acgcgctggg gatggacctg atcggcatcc aggaccaccc ttaccagagc cgctttatgg
     1021 atacctggac cctgctgtcg gctttagccg cccagacggc gcgaatccgc ttttttccgg
     1081 atgtgatcaa cctgcccctg cgcccaccgg cagtgcttgc taaagctgcg gccagcctgg
     1141 atatcatcag cggtgggcgg atcgagctag gcctgggcgc cggcgcattc tgggaagcca
     1201 tcgctgccat gggtgggccg gtgcggacac ccggagaagc ggtcgccgcc ctggaagaag
     1261 ccatccaggt gatccgcctg atgtggagcg ggcagcgcgg cctgcgtttc gatgggcagc
     1321 actaccgcct gaagggcgtc cacagcgggc cggtccccgc ccacccgatc gggatctgga
     1381 tcggcgcgta tggcccccgg atgctggcgc tcaccgggcg tctgggagat ggctgggtcc
     1441 cgacttcgac ctatgcgcct ccggataagc tgccggagat gcaccggcgc atcgactcag
     1501 ccgccctcag cgccgggcga gatccgggca tgatccagcg cgtttataac ctcatgggac
     1561 agatccagga tcgtccgggg ggcgagccgt ttattgggcc ggtcgggcag tgggtggctg
     1621 agctgacccg cctggtgctg gaattcggca tggatacctt cataatcggt ttccccgaac
     1681 ctccgggaga ccagatcgag cgctttatgg ccgaaatcgc gccgcgtgtg cgcgagaatg
     1741 tcgccgggga gcggtcggtt tcccgggctt cgtaatccat cgcgcccctc ctgagcctgt
     1801 cccctccaac cccgcgtgat ccatcgcagc catcctgagc ctgcttatcc cctataccgc
     1861 cgattacttc ttcttcaaag cagaacggcc ctaccagatc tcaacccggc gccaccaata
     1921 gcaccggaag gcttgccatc tgatagttga gccttccggt gctgtatttc aaagctccat
     1981 atccaggtca cacgggcggg tggcttttat tcagccggta aggatcagtt taccggcgcg
     2041 cctttcgtcc tgcccatcca ggttagatcc tgcagctctc caaactgctc cttaaaaatc
     2101 cggtaataca gcagttcttc tttcgtattt aatagccagc cattgggcag cc
//
LOCUS       DATMXK010000367         2309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_152114, whole genome shotgun sequence.
ACCESSION   DATMXK010000367 DATMXK010000000
VERSION     DATMXK010000367.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2309
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152114"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(10..858)
                     /locus_tag="VN363_06780"
     CDS             complement(10..858)
                     /locus_tag="VN363_06780"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559123.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84252.1"
                     /translation="MSTILTQTVPKNTGWKLFLQTVIARAYPRVIGQQREKSWMFFEI
                     FLPILSVSAYVFVYRAIGAPEEYVGFVVMGGAMTAFWLNILWSMSSQLYWEKETGNLA
                     LYIMAPNSMMAVLLGMALGGLLASTLRALAVLVLGSWLFQVDYAVSSFGQLFLVFVLC
                     MAGLYGLGMMLASVFLLLSREAWHISNLAQEPVYLLSGFYFPIKSFNFLIAAGASIIP
                     LTLGMDAMRQLIFPSGASLGFLSVTIEIRILMLLTLIFVVAAKFLLAHIEKLAIREGR
                     LTESRR"
     gene            complement(855..1874)
                     /locus_tag="VN363_06785"
     CDS             complement(855..1874)
                     /locus_tag="VN363_06785"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559124.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84253.1"
                     /translation="MNLAIRTEKLSRIYKIRGGKKQEAKQLVALQDVDLEVNPGELFG
                     LLGPNGAGKTTLIKILTTLLAPSSGKAWVAGHDVMETPQLVRQRINMVSGGESSGYGL
                     LTVRENLWMFSQFYGMPSDLANQRIKQLLEIVGLADRINTKSSDLSTGLRQKMNIVRG
                     FLTDPEVLFLDEPTLGLDVGASRDVRRFIRSWIDTNPTRTLLLTTHYMVEADELCHQV
                     AIINKGKVLACDTPSNLKRRLQRDAIFRLEVSPLNGLLPETIQSLSGVRQVVHLPQDG
                     FSVLELILAEEPVLGAVVNTLTSAEIRILNLQKREPTLEDVFVELVGRSMADVETSEE
                     GKPAA"
     gene            complement(2082..>2309)
                     /locus_tag="VN363_06790"
     CDS             complement(2082..>2309)
                     /locus_tag="VN363_06790"
                     /EC_number="3.2.2.20"
                     /inference="COORDINATES: protein motif:HMM:NF015319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008725 - DNA-3-methyladenine glycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0006284 - base-excision repair [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-3-methyladenine glycosylase I"
                     /protein_id="HWQ84254.1"
                     /translation="AYIWGFVEGRPIQNTRRTLAELPPKTQLAETLSTDLLRRGFKFV
                     GPTIVYAHMQATGMVNDHTVDCFRYAALTGR"
BASE COUNT          554 a          634 c          631 g          490 t
ORIGIN      
        1 ggagaggttt catcgccggc tctccgtcag gcgaccttca cggatcgcta atttttcaat
       61 gtgggccagc aagaatttgg cagcgaccac aaagatcagg gtgagtagca ttaagatcct
      121 gatttcaatt gtgaccgata aaaatcccag gctggccccg gaagggaaaa tgagctggcg
      181 catggcatcc attcccaggg tgagcgggat gatcgatgcc ccggccgcga tcagaaaatt
      241 gaagctcttg atcgggaaat aaaaaccgga aagcaggtaa acaggttcct gagccaggtt
      301 ggaaatgtgc caggcttccc ggctgagtaa gaggaacacc gatgccagca tcatccccag
      361 accatacaga cctgccatac acagcacaaa tacgagaaat aactggccga agctggacac
      421 agcatagtca acctgaaaga gccagctgcc cagtacaaga acagccaggg cgcgcagggt
      481 ggaggccagc agcccaccga gagccatgcc caatagaaca gccatcatcg aattaggggc
      541 cataatgtac agcgccagat tgccggtctc tttttcccag tacagctgcg agctcatcga
      601 ccacaggatg ttcaaccaga aggccgtcat tgccccgccc atgaccacaa acccgacata
      661 ttcctcggga gccccgatag cccgataaac gaatacatac gcggaaacgc tcagaattgg
      721 caggaagatt tcgaaaaaca tccaggattt ctcgcgctgc tgcccgatca cccggggata
      781 ggcccgggca atcacggtct gaaggaataa cttccagccg gtgtttttgg gtacagtttg
      841 ggtcaggatt gtgctcatgc ggcaggtttc ccttcctctg aggtttcgac atctgccata
      901 ctgcgcccca ccagctcaac gaaaacatcc tccagagtgg gctcccgttt ttgcaggttt
      961 agaatgcgaa tctctgccga agtgagggta ttgacgaccg cacccaacac cggctcctca
     1021 gccaggatca gctccagcac cgaaaatcca tcctgaggaa gatggacaac ctggcgcacg
     1081 ccagataacg attggatcgt ctcaggcagc aaaccattca gcgggctgac ctcaagccgg
     1141 aagatggcat cgcgctgcaa gcgccgcttg aggttgctgg gtgtatcaca ggccaggact
     1201 tttcccttgt tgatgattgc cacttggtgg caaagctcat ccgcctctac catatagtgg
     1261 gtggtgagga gcagtgtgcg ggtggggttg gtgtcgatcc agctgcgaat gaagcggcgc
     1321 acatcccgcg aagcgcccac atccaggccc agggttggct catccaaaaa gagcacttcg
     1381 ggatcggtga gaaagccgcg cacaatgttc atcttctggc gcagaccggt agagagatcg
     1441 gatgatttcg tattgatgcg gtctgccagc ccaacaatct caagcagctg tttgatgcgc
     1501 tggttggcga gatcggatgg catgccgtaa aactgcgaga acatccacag gttctcgcgc
     1561 acagtgagca gcccataacc cgaggattcg ccgcctgaga ccatattaat ccgctggcgg
     1621 accagctggg gggtctccat gacatcatgc cccgccaccc aggctttccc gctcgatggc
     1681 gctaacaggg tagtgaggat cttgatcagg gtggttttgc ctgcgccgtt cggccccagc
     1741 aggccaaaca gctcaccggg attgacttcc aggtcgacat cctgcaaggc aaccagctgc
     1801 ttggcttctt gcttcttgcc gccgcggatc ttatagatcc ggcttaattt ttcggtgcga
     1861 attgcaagat tcatagagtt cctggtaaaa ctttcatgat tgttgggtga tcgtagggta
     1921 atcgtagggt aagcgtcaag cagtctttag agacatttga taaactgtta ctcgaagggc
     1981 gatttctctt cttctcctcc ttagtgagag ccgggagcgg cgtacccggc tctcatcgat
     2041 tctaccagtc gaagtaattt ccagactctc atttcccatt cctacctgcc tgtcagggca
     2101 gcataccgaa agcagtccac ggtgtggtcg ttgaccatcc cggtagcctg catgtgagcg
     2161 tacacgatcg tgggtccgac aaatttgaaa ccacggcgga gcaaatccgt actcaatgtt
     2221 tcggcaagtt gggttttggg gggcagctct gccagcgtgc gcctggtatt ctggatcggg
     2281 cgaccttcga caaatcccca gatgtaagc
//
LOCUS       DATMXK010000368         4852 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12818, whole genome shotgun sequence.
ACCESSION   DATMXK010000368 DATMXK010000000
VERSION     DATMXK010000368.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4852)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4852)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4852
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12818"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1077
                     /locus_tag="VN363_06795"
     CDS             <1..1077
                     /locus_tag="VN363_06795"
                     /EC_number="6.3.2.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560735.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0008716 - D-alanine-D-alanine ligase
                     activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-alanine--D-alanine ligase family protein"
                     /protein_id="HWQ84255.1"
                     /translation="GEHDVSLMSARSVLDALNRQKYAITEIFIDQEGRWWSGDNLLDA
                     LTHRQFQPSTPVTILPDPTRPGLFILEESASLTLLSRLDVVFPVLHGTFGEDGTLQGL
                     LELVDVAYVGAGVLASSLGMDKGVFKDVMRARSIPVLESKVFLRSEIEQGVDQVLELA
                     EDIAAYPLFVKPANLGSSVGITKCRSRSDLGEGLFEASRYDRRVLVERGINGREIEVS
                     ILGNNQPLASIPGEIIPAGDFYSYEAKYLDEASRLLIPAPLSPELQEQVQKIAVEAYQ
                     AIDGAGMARVDFLLDKDTGNLYLNEVNTIPGFTQISMYPKLWEASGVPYQELLDRLIE
                     LALERKQERNRSERHYRRQDTHGA"
     gene            1067..2134
                     /locus_tag="VN363_06800"
     CDS             1067..2134
                     /locus_tag="VN363_06800"
                     /inference="COORDINATES: protein motif:HMM:NF020067.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FtsQ-type POTRA domain-containing protein"
                     /protein_id="HWQ84256.1"
                     /translation="MARNTRTTTRTDPSRCISRHYDLSTSQLRLKASGSRGSQPPPVM
                     VRSSASNAAAPTRRRTRPRRRFAIALPMAGAEMQLPAIPQLHLTWRIVSLALTVLVGA
                     VLYIYWNTPLYTVKGAEISGLTRITPLEVNTVLGITGQPIFLVDAGNIQQTLETQFPE
                     FSAIETSVSLPDSVVISVTERVPVAVWRQDGRSLYIAADGVAFPSRSQEVDPSLPVIE
                     ATANPPAPAAVPTTEEILDKLYGTGEEELTVNLTPANGAETAVDRNFMKPELVANLLT
                     IAEQAPSGVPIVYSAEHGLGWTDPGGWQVYFGALDNIEMKLHVYGALLAHLDQEDTLP
                     KLISVEYVHAPYFQLEQKRDG"
     gene            2127..3374
                     /gene="ftsA"
                     /locus_tag="VN363_06805"
     CDS             2127..3374
                     /gene="ftsA"
                     /locus_tag="VN363_06805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560737.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043093 - FtsZ-dependent cytokinesis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsA"
                     /protein_id="HWQ84257.1"
                     /translation="MDESIVVGIDVGTNKICTLVGREEGQGKIRILGVGIEPSQGIKK
                     GSVMDMGAAAQAVARSIDKAERTSGLEIDSALVSLAGSNVSSTNSRGVVGVSGRVITS
                     DDLVRAVDAARAVAIPHNREIIHVIQRGFNVDGQEGIRMPVGMHGYRLEVETHIITAS
                     AASVENLRQCVAQAGVNVSQFVLNPLAAGEVVLTETERQMGVVVCDIGGGTTNMAIYI
                     DGDVWHTAVLAIGGNHISSDIAHGLRLPMSQAEEIKIEHGYAVRDEVAAHEYFNIKPF
                     GEDDTVQISRQELAEIIEARGEEILSMVVQEIKRSGYDGLLPAGMVLTGGSSLLPGLR
                     NMASQVLGVPVRIAKPENLLGMVDQLDSPAYSTSVGLLKWATLFSEVAPQQGSSRRQS
                     PANQSAANPTMQKILSFLRRMLP"
     gene            3519..4673
                     /gene="ftsZ"
                     /locus_tag="VN363_06810"
     CDS             3519..4673
                     /gene="ftsZ"
                     /locus_tag="VN363_06810"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560738.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_process: GO:0000910 - cytokinesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cell division protein FtsZ"
                     /protein_id="HWQ84258.1"
                     /translation="MSGTNFNLPNQIESFARIKVVGVGGGGCNAVNRMIDEGLQGVDF
                     VAVNTDAQALMQSKATTRVRIGEKLTRGLGSGGDPDTGRKAAEESSEQMYEVLRGADM
                     VFITSGMGGGTGTGAAPIMAQIAKEVGALTIGVVTRPFTFEGSRRIQSAEAGIAKLKE
                     QSDTLIVIPNDRLLQIVDKRANLNQAFRMADDVLRQGVQGISELITVPGLINLDFADV
                     RTIMSEGGAALMAVGSAKGEEAASKAAEQAISSQLLDITIDGAHGILFNVTGGSDLTL
                     YDVNQAAAIIKETAHPDVNLIFGAVIDPNMKDEVRITVIATGFERTGMPHRMSDPKAH
                     EHASEARYIAERANSVPVAPPPQKPASDFQPRAFNTEDLDIPTFLRNRSR"
BASE COUNT         1096 a         1325 c         1368 g         1063 t
ORIGIN      
        1 ggcgagcatg atgtttcgct catgtcagcg cgctccgtac tcgatgctct caaccgccag
       61 aaatatgcca tcactgaaat ctttatcgat caagaaggcc ggtggtggtc aggcgataat
      121 ctcctggatg ctctcaccca ccgccagttt caaccttcta ctccggtcac aatcctgccc
      181 gatcctaccc ggccaggtct cttcatcctt gaggagtcag cctccctgac gctgctttct
      241 cgccttgatg tggtatttcc ggtgttacat ggcactttcg gagaagatgg tacgctgcaa
      301 ggcttgctcg aactggttga tgtagcctat gtgggtgcag gggtgctggc ctccagcctg
      361 gggatggata aaggcgtatt caaggatgtg atgcgcgctc gttccattcc agtcctggaa
      421 tcaaaagtat ttttacggag tgaaattgag caaggagtag atcaggtgct cgagctagcc
      481 gaggacatcg cagcttatcc tttattcgta aaaccagcca atctcggttc atcggtaggc
      541 atcaccaaat gccggtcacg atccgatctg ggcgaaggtc ttttcgaggc cagccggtac
      601 gaccggcggg tactggttga aagagggatc aacggccgcg aaattgaagt cagcatcctg
      661 ggaaacaacc agccgctggc ctccatcccg ggagaaatta ttccggcagg cgatttctac
      721 tcttacgagg ccaaatatct ggatgaggct tcccgcttgc tcatcccggc ccccctgtct
      781 cccgaattac aggagcaggt gcagaagatc gctgtagaag cataccaggc gatcgatggt
      841 gcaggcatgg cgcgggtgga tttcctgttg gacaaagata ctggcaattt gtacttgaat
      901 gaagtcaata ccatacctgg atttactcag atcagtatgt atccgaaact ttgggaagcc
      961 agcggcgtcc catatcaaga gttgttggac aggctgatcg agctggcgct ggagcgcaag
     1021 caggaacgga atcgctctga gcgacattac cggaggcagg atactcatgg cgcgtaacac
     1081 tcgaaccaca acccggacag acccttctcg gtgtatcagc cggcattatg atctgtcgac
     1141 ttcgcaattg cgcctgaagg ccagcggttc gcgcggcagc caaccgccgc ccgtaatggt
     1201 gcgcagctca gccagcaatg cagcagcccc gacacgccgc cgcacgcgcc cccgccggcg
     1261 ctttgcgata gctctcccga tggctggagc tgagatgcag cttccagcca taccccagct
     1321 gcatttgacc tggcggatcg tatccctcgc cctgacggtt ctggtaggcg cagtgctcta
     1381 tatctactgg aatacaccct tatatacagt taaaggggct gaaatcagcg gcctgacccg
     1441 cataaccccc ctggaagtta acacggtcct ggggatcacc ggacagccga tctttctggt
     1501 agacgccgga aatattcagc agaccctgga aacccagttt ccggaattct ctgccatcga
     1561 aaccagtgtc agcctgccag attctgttgt gatttccgtg acagaacgtg tcccggtcgc
     1621 agtctggcgt caggatggac gctcactcta catcgcagcg gatggggttg ccttcccttc
     1681 ccgcagtcag gaagtggatc cctccttgcc ggtgattgaa gccactgcca atccaccggc
     1741 gcccgccgca gtgccaacaa cggaagagat cctggataag ctgtatggaa caggtgagga
     1801 agaattgacc gtaaatctca ccccggcgaa tggcgctgaa acagctgtag atcgaaactt
     1861 tatgaaacca gagctggtcg ctaatctgct aacgattgca gagcaagctc cgagcggtgt
     1921 gccgattgtg tacagcgccg agcacggcct tggctggacc gatccaggtg gctggcaggt
     1981 ctacttcgga gccctggata atattgaaat gaaattgcat gtgtatggag cgctgcttgc
     2041 acaccttgac caggaagata cgttaccaaa attgatcagt gtcgagtatg tgcatgcccc
     2101 gtacttccag ttggagcaaa aacgggatgg atgaatcgat tgtcgttggc atagatgttg
     2161 gtacgaataa aatatgcact ctggtcgggc gtgaggaggg ccagggtaaa attcgcatcc
     2221 tgggggtagg aatcgaacca tcccagggta tcaagaaagg gtccgtcatg gatatgggcg
     2281 ctgcggccca ggcagttgcg cgctcgattg acaaagccga gcggacctcg ggattggaaa
     2341 ttgactcggc attggtcagt ctggctggct ccaatgtatc ttcgactaac agccgggggg
     2401 tcgtcggtgt ttcaggacgc gtgatcacca gtgatgatct ggttcgagca gttgatgcag
     2461 cccgcgccgt ggccattccg cataatcgtg agatcatcca tgtcatccag cgcggattca
     2521 acgtggacgg gcaagaaggt atccgcatgc cggtcgggat gcatggctac cggctggagg
     2581 ttgaaaccca tatcattact gcttccgcag ccagcgtaga aaatctccgc caatgtgttg
     2641 ctcaagccgg agtaaatgtc tcccagttcg tcctcaatcc cctggctgca ggtgaagttg
     2701 tgcttaccga aactgagcgg cagatgggag tggtggtctg tgatatcggc ggtggaacaa
     2761 ccaatatggc aatctatatc gacggggatg tatggcatac cgcggtcctg gcgattggtg
     2821 gcaatcacat ctcatccgat atcgctcacg ggctgcgttt acccatgtct caggccgaag
     2881 agatcaagat tgagcacgga tacgctgtgc gcgacgaagt cgctgcccac gaatacttca
     2941 atatcaaacc ttttggagag gatgatacgg tccagatcag ccggcaggag ctggctgaga
     3001 ttattgaggc gcgcggggaa gaaattcttt ccatggtggt acaggaaatc aaacgttccg
     3061 gttatgatgg tttgctgcca gccggaatgg tactgaccgg tgggtccagc ttgctgcctg
     3121 gtctgcggaa tatggccagc caggttctgg gcgtcccagt acgcattgcc aagcctgaaa
     3181 acctgcttgg catggtcgat cagctcgatt cacccgcata ttcaaccagt gtgggtctgc
     3241 tcaaatgggc tactttattc agcgaggtgg ctccccagca aggcagcagc cggcgacagt
     3301 cgcccgcaaa ccagtctgcg gccaatccaa ccatgcagaa gatcctgagt ttcttacgcc
     3361 ggatgctgcc gtaggagttg gaatatgatc cgcgggcact ttaccttagc aatcccatat
     3421 gtcgatggag tgtgcactgt tactggcaca gctgcatccc acccatatga atctttattg
     3481 cacctacagt gtaggtaacc atccttgagg aggaagtcat gtcaggcacc aattttaatc
     3541 tacccaatca gatcgaatcg tttgcacgca tcaaggttgt cggtgtgggc ggtggtggct
     3601 gcaatgctgt caaccgcatg atcgacgaag gtctacaggg tgtagacttc gtggctgtca
     3661 acactgacgc ccaggccctg atgcaatcca aagccaccac ccgcgtgcgg attggtgaaa
     3721 aacttactcg cggtctgggt tctggaggcg atccggatac tggacgtaaa gccgcggaag
     3781 aatcatcaga gcaaatgtat gaggtgctgc gcggcgccga tatggtgttc atcacatcgg
     3841 gtatgggtgg cggcaccggc accggcgcag ccccaatcat ggcgcagatc gccaaggaag
     3901 tcggcgccct gaccatcggt gtggtaaccc ggccgttcac cttcgaaggc tcacggcgga
     3961 tccagtcagc tgaggcaggc attgccaagc ttaaggagca gtcagatacc ctgatcgtga
     4021 tccccaacga tcgcctgctg cagatcgtag acaaacgcgc taatcttaac caggctttcc
     4081 ggatggccga tgatgtcttg cgccagggcg ttcagggcat ctctgagctg atcactgttc
     4141 ccggcctgat caacctggac ttcgctgatg tgcgcacgat tatgtccgag ggcggggctg
     4201 ccctgatggc tgtaggcagc gccaagggtg aagaagctgc gagtaaagcc gcagaacagg
     4261 cgatctccag ccagctcctc gatatcacca tcgacggtgc ccatggcatc ttattcaatg
     4321 tgaccggcgg ttcggattta accctttacg atgtgaacca ggctgccgcg atcatcaaag
     4381 aaaccgcaca tccagatgtc aacctgatct tcggcgcagt catcgacccg aacatgaagg
     4441 acgaagtccg catcacagtc atagccaccg ggtttgagcg gactggcatg ccccaccgca
     4501 tgtcagaccc taaagcccat gagcatgcca gtgaagctcg ctatattgct gagcgggcta
     4561 actctgttcc ggttgcaccg ccgccgcaga aacccgcatc ggatttccaa ccgcgggcgt
     4621 ttaataccga agatctggat ataccgacat tcttgcggaa ccgcagtcgc tagcgttgct
     4681 gaccgggtgg gcgcatccca ctcttaaaag tggacctgcc tgagccgtat gggctgaccg
     4741 gttaacgacg cgcggctcag cctattccag aaaaccagcc atgaccggac tcctcccggc
     4801 tatgcttggt tgcctggaat attgccagca tgcggattct gtccagcctc ct
//
LOCUS       DATMXK010000369         7147 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_5274, whole genome shotgun sequence.
ACCESSION   DATMXK010000369 DATMXK010000000
VERSION     DATMXK010000369.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7147)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7147)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7147
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5274"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..638)
                     /locus_tag="VN363_06815"
     CDS             complement(<1..638)
                     /locus_tag="VN363_06815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84259.1"
                     /translation="MSLIQSGPFPGQPQGITGWIGWLLLLAYIIYLLFHWRKLNIPWG
                     GNQTIIMILLTLSVPLSSLFIGVRLPLISSLPEPMLPLDSSSPIVVLLSALPWMLAAG
                     LLGMFPAAFIGFLAGLLQTLWETHNPFTVLEITLLAILFTAAIRQRYRTLFYRFLRHP
                     LGAAAGICVVYIFTSLFTFPLSAHGLLAARIDYTLNQLGGLWVAMTLELLLAG"
     gene            complement(775..1512)
                     /locus_tag="VN363_06820"
     CDS             complement(775..1512)
                     /locus_tag="VN363_06820"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWQ84260.1"
                     /translation="MKIKSSNPKTATSSGYKLAHREPTRRVLRFLLRVFGYTLLAKLD
                     RVEGLENVPMEGPGIVIMNHIAFIDSLTIIHVLPRHIVPLAKVEVYDYPGIGIIPRLW
                     GVIPVHREEIDRRAVQIALEVLAAGEMILVAPEGTRSPALQRGKVGVAYLAARADVPV
                     IPVAIDGSKGFPALRPFGAWKTPGAVVRIGKPFKYRPEYRKARHEELAKMTDEAMYII
                     SEMLPEERRGVYHDLTQASQDTITRLT"
     gene            complement(1493..2179)
                     /locus_tag="VN363_06825"
     CDS             complement(1493..2179)
                     /locus_tag="VN363_06825"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWQ84261.1"
                     /translation="MPELTSGRRGLRRISKWFIQGIVRLFIHLSVQGLENIPRQGPLL
                     MVSNHLGDADAFIGIAVSPVQVEMIAKIELYDIPFLGKLMDAYGVIWIHRGQPDRHAL
                     QAALQGLKAGRVIAIAPEGRESLTGSLEEGTSGAAYLGLKSGAPILPVTFTGTENRTI
                     FHNLKNLKRSPVTVTIGKVFSLDMQFERKESLQKATDQIMFILASQLPVQYQGVYSEE
                     KKALDEDQIQ"
     gene            complement(2190..2807)
                     /locus_tag="VN363_06830"
     CDS             complement(2190..2807)
                     /locus_tag="VN363_06830"
                     /inference="COORDINATES: protein motif:HMM:NF013702.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWQ84262.1"
                     /translation="MTGFENVPAHGPYIVAFNHVSLYDPPFCLAFWPVAPEAAGAADL
                     WGKPGISTLARLYGGIRVHRGQYDRKLIDTLVSALAAGRPVLIAPEGTRSHAPGMNRA
                     LPGIVAVTDRARVPVIPVGIVGTTDELLRNALHFKRPGVEMRVGKPLLLSPLEGRGEN
                     RRQMRQAQVDEIMLRIAQLLPPEYRGVYGEEFNRPRQTADDQNPA"
     gene            complement(2932..3624)
                     /gene="cmk"
                     /locus_tag="VN363_06835"
     CDS             complement(2932..3624)
                     /gene="cmk"
                     /locus_tag="VN363_06835"
                     /EC_number="2.7.4.25"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559505.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004127 - cytidylate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="(d)CMP kinase"
                     /protein_id="HWQ84263.1"
                     /translation="MSQIQIIAIDGPAASGKSTLGKKLADALGYLFFDTGVMYRAITW
                     VAIQRDLDLMDEGALTRLAEQVDIDILPASVADGRSSDILADGNDITWSIRTPEVDSH
                     VSRVSAYPGVRRALSVQQRRIGLRGRVVMVGRDIGTVVLPEAQLKIYLDASAEERARR
                     RYDELTSRGEPANYPEILAAMKRRDHIDSTRSIAPLRPAQDAIILNSDALSAEEVLAE
                     VIHMVRNPDGTA"
     gene            complement(3629..5365)
                     /locus_tag="VN363_06840"
     CDS             complement(3629..5365)
                     /locus_tag="VN363_06840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84264.1"
                     /translation="MMDFSKIKLGHLVFFTALLLAGFVRFLNLGEAPLNDSEARLALD
                     AWRVAQGVSDVGEFNPAPYPGYILVTGLLFGLLEPTNFLARFLPALAGVSLLLVPILL
                     RDRLGMPAALVLAFGLALDPGLVAASRLVAGPMPALAFGLLAGIAWQQGRPRLAGLLG
                     ALLLLSGPPAMQGIVIVLATFILLRFLPDPDESTSAQPAIKQGTSSQQIQALGVLVIS
                     LLAIGTLFLQIPQGLAALANSFLAYFSGWAGSSGVPAGRVAAALLIYQPVALIFMLLS
                     IARTLYRRQVDYLDLLLIFATGIALLHGLLYPDRQTLDLVWVLTPMWLLASRELIRQI
                     DQSQQHLAAYGLGLLIVLLAGLLWFNLAGLSRADPQLNNATARLLLMAGVVLLAAVTT
                     ILVTMGWTWKNGKFGLVLGSIGAGSLYLISVLWGSTQVRANLPAELWYSGGAGSQADL
                     LAKTTHELSVWTTGFPDQVEIVLKGESPSVQWVLRDFSELKIVDIIPTQDLPAILITP
                     QTEQPPALTAAYRGQDFNWWVYPGWVNALPENLPAWITFREAPLTQKQIILWARSDLF
                     PGGELEISNSEN"
     gene            complement(5410..>7147)
                     /locus_tag="VN363_06845"
     CDS             complement(5410..>7147)
                     /locus_tag="VN363_06845"
                     /inference="COORDINATES: protein motif:HMM:NF021570.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DUF2298 domain-containing protein"
                     /protein_id="HWQ84265.1"
                     /translation="GSRVLQDLDLANQPLGLSPITEFPFFSFLLGDNHPHVLALPFVL
                     LSIGLGVNLLLRGFETRPADSANYPREWWNPVRTVFGGSWLDFGFYALALGALGFLNT
                     WDMPVYIALVTLAFGVGGAMRRRVLNWDLVRRGMGLAIGLLLASVALYGLFYAAFRSQ
                     AGGILPYVFPPTRLPQYLVMFGPFILIILGLLVALAMRHRKQYHTGSMLKSSLRTWLA
                     VIGVWVGFFGLLLIAILASGLGRQMLNSGQVDPILQPVLGNLSLTEAFRSILISRLTN
                     PWLFLLVTLLIGMIIAILAQWIQPENQAGLQSARPVPSPSEAFVLIMIFTGLVLTFSV
                     EFVYLRDTFMARMNTVFKFYYQGWILLALSAAYGLWWISQNGEKVLGSLAFRLGQILA
                     LLSIAGGLVYPVFATYSRVAGFRFPPSLDGASNLAAYNPDDWAAIDWLNQQTQNRLAE
                     GQTDLPIILEAPGKSYNYEGRISAFSGVPAQLGWSLHESQWRGNYDEQGRREPDIDTI
                     YTSTDTFVTQELLNKWDIDYVILGSAEMQYIQQLCEGTNRACNLSRSINKFETMFQAV
                     FQQGSTTIFAVP"
BASE COUNT         1802 a         1922 c         1886 g         1537 t
ORIGIN      
        1 cctgcaagca ggagttctaa ggtcatcgct acccataatc caccgagttg gttcagggtg
       61 taatcgattc gagctgccag caacccatgg gcagataacg gaaaagtaaa taatgacgta
      121 aagatataaa ccacgcagat gccggcggca gcccccagag gatggcgaag gaagcggtag
      181 aataatgtcc ggtaccgctg gcggatggca gctgtaaaca gtatcgccag cagagtgatt
      241 tctaaaacgg tgaatgggtt atgggtttcc cataatgtct ggagcagacc ggccagaaac
      301 cctataaaag ctgccgggaa catccctaac agaccggcgg ccagcatcca gggcagagct
      361 gatagcaaga ccacaatcgg gctggagcta tccagaggca gcatgggttc aggcaggctg
      421 ctgatgagtg ggaggcgtac tccaatgaaa aggctgctga gcggcacact taatgtcagc
      481 agaatcatga tgatggtctg gtttccaccc caggggatat taagcttccg ccaatgaaac
      541 aacagataga tgatgtaagc cagaagcaat aaccacccga tccaccccgt aattccttgg
      601 ggctggccgg ggaagggacc tgactgtatt aatgacatga tcaatttcat aatctactga
      661 cccgttgcaa gttcaattcc actcccgcct tttctttaga tctcattata tcatgatcga
      721 atttggctga gagcaataaa catttcgaat aaacagtgcc ttaccggtcg aaaattaagt
      781 aagcctggta atcgtatcct gggaagcctg cgtcaggtca tggtagacgc cgcgccgttc
      841 ttcagggagc atttccgaga taatatacat ggcttcgtct gtcattttcg ccagttcttc
      901 gtgccgcgct tttcgatatt cgggacggta tttaaatggt tttccgattc gaactactgc
      961 tccaggtgtt ttccatgcgc caaacgggcg tagagccgga aagccctttg atccgtcaat
     1021 cgctaccggt atgactggca catccgcccg tgctgccaga taggcaactc caactttgcc
     1081 ccgctggagg gcgggacttc gcgtcccttc tggtgccacc aggatcatct cgccagcagc
     1141 taacacttcc aacgctatct ggacggctcg ccggtcaatt tcctcacggt gaactggaat
     1201 aactccccac agtctgggaa taatgccaat ccctggataa tcgtatactt caacttttgc
     1261 gagcggtacg atatggcggg gcagcacatg gatgatagtc agtgaatcga tgaacgcaat
     1321 atggttcatg ataacgatcc ctggaccttc catgggtacg ttttcaagac cttcaacccg
     1381 atccaactta gccagtaaag tgtaaccaaa caccctcagc aaaaagcgca gcacacgccg
     1441 ggttggctct cgatgggcta atttgtatcc gctcgaagta gctgttttgg gattactgga
     1501 tttgatcttc atccagggcc tttttttcct ccgaatatac accttgatat tgaaccggta
     1561 gctggctggc caggataaac atgatctgat cggttgcttt ctgtaagctt tccttgcgtt
     1621 cgaactgcat atccaacgaa aacacctttc cgattgttac tgtgacaggg ctgcgtttca
     1681 aattcttaag gttatggaaa attgtccggt tttctgtgcc cgtaaaagtc acgggtaaga
     1741 tgggggcgcc gcttttcaat cccaggtagg cggcaccact ggtgccttcc tcaaggctgc
     1801 cggtcaggct ttcacgacct tctggggcga tcgcaattac acgtccggcc ttgagcccct
     1861 gaagcgctgc ctgcagagca tgccggtcag gttggccgcg gtggatccag atcacaccgt
     1921 aagcatccat cagcttgccc aggaaaggaa tgtcatataa ttcgattttt gcgatcattt
     1981 caacctgaac aggcgagacc gcaatgccga tgaacgcatc tgcatctcca agatgattgg
     2041 aaaccattaa taaaggaccc tgtctgggta tgttttccag gccctggacc gataagtgga
     2101 tgaagagcct gacgataccc tgaataaacc atttgctgat cctgcgtaag ccgcgtcgcc
     2161 cggaggttaa ctcaggcaaa cgagtaagct caggccggat tttggtcgtc cgcggtttgt
     2221 ctgggacggt tgaattcttc tccatatact ccccgatatt caggtggtag taattgagcg
     2281 attctcaaca ttatctcatc aacctgtgcc tgtcgcatct ggcggcggtt ttcaccccga
     2341 ccttccaggg gtgagagcag cagtggcttt cccacccgca tttcgacgcc cggtcgtttg
     2401 aaatgcagtg cgttgcgcag caattcatcg gttgttccca cgatccccac cgggatcacc
     2461 ggaacccttg cccgatccgt aacggccaca atcccgggta gggcacggtt cattccaggc
     2521 gcgtgtgagc gggtaccttc tggagcgatt aacacgggtc gaccagcagc cagagcagag
     2581 accagtgtat cgatcagttt ccgatcatat tgaccgcggt gaacccggat gcctccatat
     2641 aagcgggcca gtgtagagat tcctggtttt ccccacaggt cggctgcccc ggcagcctct
     2701 ggggctacgg gccagaaagc caggcagaaa ggtgggtcat ataacgacac atggttgaac
     2761 gctacgatat acggtccatg tgctggaaca ttttcaaagc cagtcaacgt gacttgcatg
     2821 aataaacgaa aaaaagctcg aaataaaggc cgtaatatac cgcgcttcag gcgagtatgc
     2881 agaggcaagt gataccgatc ctgggttgaa tccggagtgt gcgattcgaa ctcaggctgt
     2941 gccatcggga tttctcacca tgtggatcac ttctgcaagc acctcttcgg cggatagcgc
     3001 atccgaattt agaataattg catcctgggc tggacgcaga ggggctattg agcgagtcga
     3061 gtcgatatga tcgcggcgtt tcattgcagc caggatttcc gggtaattgg ccggctcacc
     3121 gcgggaggtt aattcatcat accgccgtct ggctcgttcc tcggcagacg catccaggta
     3181 aatttttaac tgtgcttcgg gaaggaccac agtgccaata tcgcggccga ccatcacgac
     3241 cctgccgcgc agaccaatcc ggcgctgctg cacgctgagg gcacggcgga cacccgggta
     3301 tgcagaaact ctggatacat gggagtctac ttcaggagta cggatcgacc aggtaatatc
     3361 gttcccatct gccaggatat ccgagctgcg cccatctgcc acactagctg gcaggatatc
     3421 gatatcgacc tgctcagcca gccgggtaag ggcaccctca tccatcagat caagatcgcg
     3481 ttgaatagcc acccaggtga tggcacgata catcactccg gtgtcaaaaa ataaataacc
     3541 cagcgcatca gcaagctttt tgcccaaggt agattttccc gaggctgccg ggccatcgat
     3601 cgcaataatt tggatttgtg acattctgtc aattttccga atttgagatt tctagctcgc
     3661 cacccgggaa caggtcagat cgagcccata aaattatttg cttttgcgtc agcggtgctt
     3721 cccgaaatgt gatccaggct ggtaaatttt ccggaagggc atttacccag ccaggataga
     3781 cccaccagtt gaaatcttgc ccgcgataag ccgccgtcaa cgccggaggt tgttcagtct
     3841 gaggagtaat caggatcgct ggtaagtcct gggttgggat aatatctacg atttttaatt
     3901 ccgaaaagtc acgtaaaacc cattgaacgg agggtgattc acctttcaga acaatttcca
     3961 cctgatctgg aaatccagtt gtccaaaccg aaagctcatg ggtggttttt gcgagtaaat
     4021 cagcctggct gccagcgccg cccgaatacc ataattctgc cggtaaattg gcacgcacct
     4081 gcgtgcttcc ccacaaaaca ctgatcaaat acaggcttcc cgcgccgata gaccccaaca
     4141 ccagaccaaa tttgccgttc ttccacgtcc aacccatggt taccagaatg gtagtgacgg
     4201 cagccaatag aaccactcca gccatcagga gcaggcgagc tgtggcgttg ttgagctgtg
     4261 gatcggcccg gctgagaccg gcgagattga accacagcaa gccggctaat aaaacgatca
     4321 acaatcccaa tccataggca gccaggtgct gctgactttg atcgatctgg cggatcagct
     4381 cccgtgaagc caggagccac attggagtca acacccagac taaatccaga gtctggcggt
     4441 ccggataaag aagtccatgc agtaaggcta tcccggtcgc gaagatcaac agcaggtcca
     4501 gataatccac ctggcgtcga taaagcgtcc gggcgatgct tagcagcatg aagatcagag
     4561 ctaccggctg gtaaattaac agtgcggctg ccacccggcc tgccgggaca cccgagctgc
     4621 cggcccagcc gctgaaatat gccaggaagg aatttgccag agctgccagt ccctggggaa
     4681 tttgcaggaa aagggtgccg atagcgagca ggctgattac cagtacaccg agagcctgga
     4741 tttgctggga tgaggttcct tgcttgatgg ctggttgggc acttgttgac tcatccgggt
     4801 cgggcaagaa tcggagcagg atgaaagttg ccaacacaat aacgatccct tgcatcgccg
     4861 ggggaccgga gaggagcagc aatgctccca gcaatccagc caggcgcggc ctgccttgct
     4921 gccaggcgat tcccgccagt aacccgaatg ccagcgccgg catggggccg gcaacgagcc
     4981 gggaggcggc gaccaatccg gggtccagcg ccaggccgaa tgccagaacg agcgcggcgg
     5041 gcatcccgag gcgatctcgc agcaggatcg ggaccaggag caaggatacc cctgccaggg
     5101 caggcaaaaa gcgcgccagg aaatttgtcg gttccaggag gccgaaaagc agcccggtga
     5161 ccaggatata accaggatac ggggcggggt taaattcacc cacatcactg acaccctgag
     5221 cgacgcgcca ggcatccagc gccaggcgcg cttctgaatc atttaatggc gcttcgccta
     5281 aatttaagaa acgcacaaaa cctgccagga gcagggcagt gaaaaataca aggtgtccga
     5341 gtttaatttt gctgaaatcc atcattaccc ggggatcgaa aataatactg atacggattc
     5401 taccagtgat caggggactg cgaagatcgt ggtgctgccc tgttggaata ctgcttgaaa
     5461 catggtctca aacttattga ttgagcggct taggttacag gcccgattgg taccttcaca
     5521 aagctgctgg atatactgca tctctgctga ccccaggatc acataatcga tatcccattt
     5581 gttgagaagc tcctgggtta caaacgtgtc ggtggaagtg tagatggtat cgatatccgg
     5641 ctcgcgtctg ccctgttcat catagtttcc tcgccactgg ctctcgtgca aggaccatcc
     5701 cagctgcgcc ggaacaccac tgaaagcgct gatccgaccc tcatagttat aggactttcc
     5761 tggtgcttca aggattatgg gaaggtcagt ctgtccttcc gcaagccggt tttgggtttg
     5821 ctggtttagc cagtcaatgg ccgcccaatc atctggattg taggctgcca ggttgctggc
     5881 gccatccagg ctgggtggaa atcgaaatcc ggcgacccgg ctgtaagtgg caaagactgg
     5941 atataccaat ccgcctgcga ttgagagcag cgccagaatc tgcccgaggc gaaacgccag
     6001 gctgcccaaa accttttccc cgttttgact gatccaccat aatccatagg cagccgataa
     6061 tgccagcaag atccagccct ggtaatagaa tttgaacacc gtgttcatgc gtgccataaa
     6121 ggtatcacgc aggtaaacaa attccactga aaaagtcagc accagaccgg tgaagatcat
     6181 gatcagaaca aatgcctcac tgggagacgg gactgggcgc gcagattgca agcctgcctg
     6241 gttttcaggt tggatccact gcgcgagaat ggctatgatc atgccaatca ataaagtgac
     6301 caacagaaac aaccagggat ttgtaagccg cgagatcaag attgacctga atgcctcggt
     6361 gaggctcaga ttgccaagca caggctgtaa gattggatca acctgcccac tgttgagcat
     6421 ctgcctcccc aatccggaag ccaggatagc gatcaaaagc agtccgaaaa agcccaccca
     6481 taccccaatc actgccagcc aggtgcgcag gctcgatttg agcatgctcc cggtgtgata
     6541 ttgtttcctg tgccgcatag ctagagcaac gagcagcccc aggataatca gaatgaacgg
     6601 cccgaacatc accaggtatt ggggcaaacg ggtcggtgga aagacatagg gcaatatccc
     6661 tccggcctgc gagcggaatg ctgcatagaa caatccatac aaagccacac tggccagaag
     6721 cagtccgatt gccagaccca ttccccggcg gaccaggtcc caattgagca ctcggcggcg
     6781 catggctccc ccgactccaa aagcaagggt caccagggca atatagacgg gcatatccca
     6841 cgtatttaga aatccaaggg ctcccaatgc gagggcataa aacccaaaat ccagccagct
     6901 tccaccgaaa actgtacgaa ccggattcca ccactccctg ggatagttcg cagaatcagc
     6961 tggtctcgtc tcgaaacctc gcaaaagtag attcacgccc agaccgatac tcaagagcac
     7021 aaatggcagc gccaggacgt gtgggtgatt atcacccagt aaaaagctga agaaagggaa
     7081 ctcggtgatt gggctgagcc cgagaggctg gttggcaagg tccaggtcct gcaggacacg
     7141 tgatccc
//
LOCUS       DATMXK010000370         6231 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_92182, whole genome shotgun sequence.
ACCESSION   DATMXK010000370 DATMXK010000000
VERSION     DATMXK010000370.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6231)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6231)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6231
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92182"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..899
                     /locus_tag="VN363_06850"
     CDS             <1..899
                     /locus_tag="VN363_06850"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84266.1"
                     /translation="TQPLWLGERSGAGPIDIQREFTSPIRLVVRIISSDSGSRHPRLV
                     IHGTSSTGNNRIEEVTEERFKWLLGRGNLTGERIYQAIDRLEIENIEPDDRVIVQTAG
                     YQSTTITQLLPLWTGQLPTPITDQLVNGSIINPQRFWKPFGLPACIDEPLIPENDLCI
                     SCEPVWNSFIIAGLLAAGYLQPAAELFTRLMDAIVLSLKSESGFRQYYNPNTGQGMGE
                     LNHLNGLPPVGLFLRLLGIQIHSPTNVEIYGENPFSWPVTVQYRGLTILRGKDKATII
                     FADGQTVELTGSTPQVVSLETM"
     gene            929..1318
                     /locus_tag="VN363_06855"
     CDS             929..1318
                     /locus_tag="VN363_06855"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HWQ84267.1"
                     /translation="MEASPDHSTPTLPSQSASAERIDRLMIAFIQTQDLDKAMRALNK
                     LRFSVTQVSTTGGFLGQNNSTLLIGFPRAKESLAFEALQKSCRRRVEYAATPLEGAPY
                     HMPLATPIPVGGATVFTFDIEHYEEIG"
     gene            1315..1581
                     /locus_tag="VN363_06860"
     CDS             1315..1581
                     /locus_tag="VN363_06860"
                     /inference="COORDINATES: protein motif:HMM:NF017920.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic-di-AMP receptor"
                     /protein_id="HWQ84268.1"
                     /translation="MKLIIAIVRDIDKDPVSDSLLEAQFRVTQVSSTGGWLRRGSSTL
                     MIGVEDEKLDKAMQIIRSHLDPSSTTESKKATLFVLNVDHHTQI"
     gene            complement(1614..2198)
                     /gene="amrA"
                     /locus_tag="VN363_06865"
     CDS             complement(1614..2198)
                     /gene="amrA"
                     /locus_tag="VN363_06865"
                     /inference="COORDINATES: protein motif:HMM:TIGR00296.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein A"
                     /protein_id="HWQ84269.1"
                     /translation="MQDPLTITEKQQLLAVARQALEAAVNRLPLPPLQPEQFSDRLQD
                     FGACFVTLTCAGELRGCIGSLEAYQPLVEDIRQHAIAAATQDYRFQPIAPEELGNIEI
                     EISYLNAPQPVDYKTPTELLDILRPGTDGVIIRDGLRRATFLPQVWEKIPDKMMFLSH
                     LCEKMGAPYDLWLKKPLQVFVYQVDEFKETQHSN"
     gene            complement(2224..2724)
                     /locus_tag="VN363_06870"
     CDS             complement(2224..2724)
                     /locus_tag="VN363_06870"
                     /inference="COORDINATES: protein motif:HMM:NF013756.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavin reductase family protein"
                     /protein_id="HWQ84270.1"
                     /translation="MTNNIEPLDPDEFRTAMRRWATGVTIVTAQHDSIQHGMTVSSFT
                     SISLEPPLVMVSLGQTTKTIQLIHASGFFGVSILAEDQQRISDSFAGRLPEVEYKFDS
                     VDWTTLTSGSPLLVSGLASFDCRVISAQDFGMQTIFIGEVVGLRLGKNSNPLIYFERD
                     YHQVVG"
     gene            2983..3264
                     /gene="rpsF"
                     /locus_tag="VN363_06875"
     CDS             2983..3264
                     /gene="rpsF"
                     /locus_tag="VN363_06875"
                     /inference="COORDINATES: protein motif:HMM:TIGR00166.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S6"
                     /protein_id="HWQ84271.1"
                     /translation="MRAYELVVIVHPDLDETALGEVVERIKGWVTVDGGSIDKVDMWG
                     RRRLAYPIRKQLEGQYVFFQTSMLEKNLVGLERNLRFLEPVMRFLLIAN"
     gene            3320..3712
                     /locus_tag="VN363_06880"
     CDS             3320..3712
                     /locus_tag="VN363_06880"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560636.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWQ84272.1"
                     /translation="MTRGLNKVMIIGRLGRDPEMRYTPSGRPVTTFSVATSRSWNTSE
                     GGRRTETEWFNVVAWSTLAEICKQHLSKGRLVYIEGRLQTRQWDDPEGTKHSSTEIVA
                     NEMIMLDDRREGNHVEDEDEIDEEEFPF"
     gene            3769..4038
                     /gene="rpsR"
                     /locus_tag="VN363_06885"
     CDS             3769..4038
                     /gene="rpsR"
                     /locus_tag="VN363_06885"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007403456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S18"
                     /protein_id="HWQ84273.1"
                     /translation="MTNQPPTPEGRNAPRRFFARPRECQFCADKNAVIDYKQSEVLRR
                     FVSDDGKIRPRRQTGTCAKHQRALAQAIKRARHLALLPYSGEVLR"
     gene            4109..5602
                     /locus_tag="VN363_06890"
     CDS             4109..5602
                     /locus_tag="VN363_06890"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF024545.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="peptidylprolyl isomerase"
                     /protein_id="HWQ84274.1"
                     /translation="MSDTPKEKIPQTKKHLARQQREDMQRRYLLIGVAVILVAVIGVI
                     LYGFLYEGPIQDSRAIVTVNGDKVTTKQFEGLVRYTRYSLIQNAANTYQLAQMFGTDP
                     SIQSSILSQMSQTQSQMESSVVGQQMLDRVTNDLLIRQEAERRGITVTEAEIDKSFQE
                     AFGYFPDGTSTPTATLEQIPTSTYSPLQMTVMAPTATLDPTVATATAEALSAAATATA
                     QAAPATAMPEVAITPTPEVTPTQIPTATSTPSGPATATPTLAPTATATPYTLEGYQNL
                     YRESITGFKDNYAISEADLRYVIESQLYYFKVFEAVVAELDVPRTQEQVWARHILLET
                     KEQAEEVMSLLEGGMDFAVLAKTYSTDTSSKDQGGDLGWFGTGFMVAPFEEAVFALEP
                     GQVSEIVETDFGFHIIQSLGKEERPLSAEDYQAEQTAKFEEWLAAQKEAATIVTLEDW
                     LSRVPDDPIMPPEISNYINELQTQFLQSQATQSAPLTAPTTAPTTQP"
     gene            complement(5722..>6231)
                     /locus_tag="VN363_06895"
     CDS             complement(5722..>6231)
                     /locus_tag="VN363_06895"
                     /inference="COORDINATES: protein motif:HMM:NF014516.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ84275.1"
                     /translation="VIRELDGLMQREFRITFEEVAGEFREIFTRMFGGGSARLVLTNA
                     DDLANTGIDIEARLPGRRSQGLSLLSGGERSLTATALVFALLKVSPTPFCVLDEVDAM
                     LDESNVGRFRDLLRELSRNTQFIVVTHNRNTVQAADVIYGVTMGRDSASQVLSLKLDQ
                     VSQVVGEAA"
BASE COUNT         1580 a         1648 c         1600 g         1403 t
ORIGIN      
        1 gcacacaacc tctctggctg ggagagcgat ccggcgccgg accgatcgat atccaacgcg
       61 aattcacttc tcccatccgc ctggtcgtgc ggattatttc cagcgattcc ggctcccgcc
      121 accctcgcct ggtcattcat gggacaagca gcaccgggaa caatcgaatc gaggaagtta
      181 ccgaagagcg atttaagtgg ctgctcgggc ggggaaacct gaccggggag cgaatttatc
      241 aggcgattga ccgtctggag atcgaaaata tagaacccga cgatcgggtc attgtacaga
      301 cagccggcta tcagagcacg acgatcaccc agcttctgcc attatggaca ggccagttac
      361 ccacacccat tacggatcag ttggttaatg gatctatcat taatccacag cgcttctgga
      421 agccttttgg tctcccggca tgtatcgatg aaccgctgat cccagaaaat gacctgtgca
      481 taagctgtga acctgtgtgg aattctttca tcattgcagg actgctcgca gccggttatc
      541 tccaaccagc ggctgagctt tttactcgat taatggatgc aatagtccta tccttaaaat
      601 ctgaatccgg attccggcaa tattacaatc caaacaccgg gcaagggatg ggtgagctga
      661 atcacctgaa cggattaccg cctgttggct tatttttacg tctgcttgga attcaaatcc
      721 attcgcccac gaacgtggaa atctacgggg agaacccctt ctcctggcca gttacggtac
      781 aatatcgagg attaaccatt ctgagaggaa aagataaggc aacgatcatt tttgccgatg
      841 gtcaaacggt agagttaacc ggctctaccc cgcaggttgt ctctttagag acgatgtaag
      901 cgtagcagcc agtagaggaa aagaaggcat ggaagcttca cccgatcact caactccgac
      961 actaccgtcc caatctgctt ctgcagagag aatagatcga ttaatgatcg cattcatcca
     1021 gacccaggat ctggataagg cgatgcgggc tctgaacaaa cttagatttt cagttactca
     1081 ggtctccaca acaggtggat ttttaggaca gaataattcc actctattga tcggttttcc
     1141 gagagccaag gaatcgctcg cctttgaggc tttgcagaag agctgccgga ggcgagttga
     1201 atatgcggct acgcccctgg aaggcgcacc ctatcatatg cccttagcca caccaatccc
     1261 agtgggcggt gcgacggtct ttactttcga tattgaacac tatgaggaga tcggatgaag
     1321 ctaatcatcg caattgtcag agacattgat aaagatccgg tttcggattc gctgctcgag
     1381 gcacaatttc gggtaaccca ggtctcctcg accgggggat ggctgcggcg gggttcaagc
     1441 acattgatga tcggtgtcga agatgaaaaa ttggataaag cgatgcagat cattcgcagc
     1501 cacctcgacc catccagcac gacagaaagc aaaaaagcca ccctgttcgt gctcaatgtc
     1561 gatcaccata cccagatcta aatcgaccgg ctgtgtattc ggaggagcag ggcttaattt
     1621 gagtgttggg tttctttaaa ctcgtcgacc tgatatacaa aaacttgcag tggttttttc
     1681 agccagagat cgtagggggc gcccattttt tcgcataaat ggctcagaaa catcatttta
     1741 tcggggattt tttcccaaac ctggggcagg aaagtggcgc gccgcaaccc atcgcggata
     1801 attacaccat ccgttcccgg ccgcaggata tccagcagct cggtgggagt cttgtagtct
     1861 accggttgag gtgcattcag atacgatatc tcgatctcaa tgttgcctaa ttcttctggc
     1921 gcgattggtt gaaagcggta atcctgggtt gcagccgcga tcgcatgctg gcgaatatcc
     1981 tcaacaagcg gctgatatgc ttccagagat ccaatacaac cgcgtaactc tcccgcacat
     2041 gtgagcgtca caaagcaagc cccaaagtcc tgcaggcgat cggaaaactg ctctggttgg
     2101 aggggaggca aaggcaggcg attaacagcg gcctccaatg cctgccgggc cactgccagc
     2161 agctgctgtt tctcggtaat tgtcagggga tcctgcattt ttccacccct tcaaagtact
     2221 tgatcagccc acgacctggt gataatcgcg ctcgaagtaa attagtggat tggaattttt
     2281 acccaggcgc aagccgacga cttccccgat gaatatagtt tgcatcccga aatcttgcgc
     2341 agaaatcacg cgacagtcaa aactggccag gccagataca aggagcggcg agcccgaagt
     2401 tagagtcgtc caatccacgc tatcaaattt atactcgact tctggtaacc ggcctgcgaa
     2461 actgtctgag atccgctgct ggtcttcagc caggatggaa accccaaaaa agccactggc
     2521 atggataagc tggatagttt ttgtggtttg tccgagcgaa accataacca gaggaggctc
     2581 taaagaaatc gaagtgaagg aactgactgt catcccgtgc tgaatggagt catgttgggc
     2641 tgtcacaatg gtcacaccag ttgcccatcg ccgcattgca gttcggaatt cgtctgggtc
     2701 taaaggttca atattattag tcacaaagta tcctcatcca aatttatcct accatcatac
     2761 attcgtgagt ctgtgacgtc aagaggttga catcgtcaag gcaaatatat caggcttgat
     2821 atttccgttc tctaaatgca agtaattaga aatatccctt tgcaaatcca tgattgtatg
     2881 ctaaactact gcgaagccac gcagcgcgtg gcttgctttc cgcctcggac cagccaacgg
     2941 ctgtatcccg gggcatatcc caaggaaagg aggcatttcc cgatgcgtgc atatgaattg
     3001 gttgtgatcg ttcacccaga cctggatgaa acggctctcg gtgaggtagt tgaacgcatt
     3061 aaaggctggg tcacagtgga cggcggaagt atcgataaag tcgatatgtg gggtaggcgg
     3121 cggttggctt acccgatccg caagcagctc gaaggccagt atgtattttt tcagaccagc
     3181 atgctcgaga agaaccttgt cggattggaa cgtaatttgc gtttcctcga gcccgtcatg
     3241 cgtttcttgc tcattgccaa ttagttgaga gttatctgcc tcaaataaaa gaggctggag
     3301 ctccccatag gagagtaaga tgacaagagg tctgaacaag gtcatgatta tagggcggct
     3361 ggggagagac cccgaaatgc gctacacacc ttctggacgc ccggtgacga cctttagtgt
     3421 agctacaagc cgatcctgga atacgtccga gggtggccgg cgaaccgaaa ccgaatggtt
     3481 taatgtggtc gcctggagca cgctcgctga aatctgcaaa cagcacctca gcaaaggccg
     3541 cctggtctat atcgaagggc gattgcaaac tcgccagtgg gatgatccag aaggtacaaa
     3601 acactcgtcg accgagatcg tcgccaatga aatgatcatg ctcgatgatc gccgggaagg
     3661 caatcacgtc gaggatgagg acgagatcga cgaagaagaa tttccatttt agggtctctg
     3721 agcagcctgt aaagcgctca aaggcaacct atcacgagga gtcagaacgt gactaaccaa
     3781 ccccctaccc ctgagggacg aaatgcccct cgccgcttct ttgcccggcc gcgtgaatgc
     3841 cagttctgcg ccgacaagaa tgcagtgatc gattataagc agtcggaagt cctgcgccgc
     3901 tttgtaagtg atgacggtaa aattcgccct cgacgccaaa ccggtacctg cgcaaagcac
     3961 cagcgtgctt tagcccaggc aatcaaacgt gctcgccatc tggcgctgct gccttattct
     4021 ggcgaagtac tgcgttaatt tgcctttatc aacatagatc gcggggcata ggattcccct
     4081 gtgccccgct tttgtgtaag gacctgatat gtctgatacc cccaaagaaa aaattcccca
     4141 gactaagaag catctggctc gccagcagcg cgaagatatg cagcggcgct atttgctgat
     4201 tggtgtggct gttattttag ttgctgttat tggcgtgatc ttatatggct ttctatatga
     4261 aggaccgatc caggattctc gggcgattgt gaccgttaat ggtgacaaag ttactacgaa
     4321 acaatttgag ggtctggtcc ggtacacacg ctattctctg atccagaatg cagccaatac
     4381 ttatcagctg gcccagatgt tcggtacgga ccccagtatc caatcatcga tcctcagcca
     4441 gatgtcccag acccaatctc aaatggagtc ttctgtggtc ggccagcaga tgctggacag
     4501 ggtgaccaac gacctcctga tccggcagga agccgaacgg cgtgggatta cggttactga
     4561 ggcggagatt gacaaatcat tccaggaagc cttcgggtat tttcctgacg gaacgtctac
     4621 cccgactgct acgctggaac agattccaac ttctacatac tctcctctgc agatgaccgt
     4681 gatggcgcct accgcgacgc tggatccaac ggttgcaact gctaccgcag aagcactcag
     4741 tgccgcggcc acggcgactg cccaggctgc cccggcaact gctatgccgg aagtagccat
     4801 tacccctacc cctgaagtaa cgcccaccca gatacctact gctaccagca ctccctccgg
     4861 accagcaact gcaacaccta ccctggcgcc gacggcaact gcgaccccgt atactctgga
     4921 agggtaccaa aatttgtacc gggagtcgat taccgggttc aaagacaact atgcgatttc
     4981 tgaagccgac ctgcggtatg tgatcgaatc acagctttat tatttcaagg tctttgaggc
     5041 agtcgtcgca gagcttgatg tcccccgcac tcaggaacag gtctgggcac ggcacatcct
     5101 gctggaaaca aaagaacagg ccgaagaagt aatgtcttta ctcgaaggcg gcatggactt
     5161 tgccgtcctg gccaagacat actcgaccga taccagtagc aaggatcaag ggggcgatct
     5221 gggatggttt gggaccggct ttatggtcgc cccatttgaa gaagcggttt tcgcgctcga
     5281 acccgggcag gtcagtgaaa tcgttgaaac cgattttggc tttcatatca tccagagcct
     5341 gggtaaagaa gaacgtccat tgagcgctga agattaccag gccgaacaga ccgcgaagtt
     5401 cgaggaatgg ctggctgcac agaaagaagc cgcaaccatt gttacccttg aggattggct
     5461 aagccgggtc ccagacgatc cgatcatgcc gccggagatc agcaactata tcaacgagct
     5521 gcaaacccaa ttcctgcaga gccaggcgac ccagtctgca ccgctcactg ccccaacaac
     5581 tgcacctacc acccagccat aatccagact gattcgtttc tgagagaaat ccggtttaac
     5641 gcttcgatgc ggatgatcgc cagaaatata acagccccga gtcacatagt aatcggggct
     5701 gttttggcgt tgtaggacag ttcaggcagc ttcgccgacg acctggctga cctgatcgag
     5761 cttgaggctc agaacctggc tggctgaatc ccgccccatc gtcaccccat agattacatc
     5821 tgcggcctga acagtattgc ggttatgggt aaccacaata aattgggtat tgcggctcag
     5881 ctcgcgcagc agatcgcgga agcgacccac gttgctttca tcgagcatgg catccacttc
     5941 gtcaaggaca cagaatggcg tgggagaaac cttgagcaga gcgaaaacca gcgcagttgc
     6001 ggtcaggctg cgctccccgc ccgagagcag agacaatccc tgtgatcgcc ggcctggtaa
     6061 acgggcttcg atatcgatac ccgtattcgc caggtcatcc gcgttggtca ggacaaggcg
     6121 ggccgagcca ccgccaaaca tacgtgtgaa aatctcgcgg aattcgccgg cgacttcttc
     6181 aaaagtaatc cgaaattcgc gctgcatcaa gccatccagc tcgcgaataa c
//
LOCUS       DATMXK010000371         2391 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_159941, whole genome shotgun sequence.
ACCESSION   DATMXK010000371 DATMXK010000000
VERSION     DATMXK010000371.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2391)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2391)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2391
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_159941"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(16..246)
                     /locus_tag="VN363_06900"
     CDS             complement(16..246)
                     /locus_tag="VN363_06900"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84276.1"
                     /translation="MATKTWEVLRVRYCHHVDKNVGFEAQVVYPADFLPDQQPRILAH
                     RCSHAFSCNLDGRASCIWAGTNPSLDLFVEEN"
     gene            585..2342
                     /locus_tag="VN363_06905"
     CDS             585..2342
                     /locus_tag="VN363_06905"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ84277.1"
                     /translation="MSRKHLLTLGSILIVFSMILSACAQATPAPTEAPAEPVATEAPA
                     EPVATEAPAEPMEESVFIFGRGADSISLDPALVTDGESFRVTGQCLEPLYQYEPGTTK
                     PIPALATACTPNDDAMEWTCNLREGVKFHDGTDFNADAVIFNYERWRFTDNPLHFESQ
                     VFEYYEAMWGGFDDASLITSVEKVDDLTVKFVLSTPMAPFLANLAMDMFAISSPAAME
                     AAGENYGTPSVGCVGTGPYKFVEWVEGDSITVEANADYWGGEPPTKKIIWRVIPDDSA
                     RFLALKAGDIQGLEQATVEDLSSAENDPELQIITRPALNTAYLAFNYKVVEFQDIRVR
                     EAVAHAVNRAGLVENFYGAYGEVAKNLLPPLVWGHNDAIEDWTYDPELSKSLLAEAGF
                     PDGLSEVTIAEDINDAEGNPIFTAGEKIPLRLYYMPATRFYYPSPKEIGEAMAADLVA
                     AGINAELYLEGDWATYLGARSNGALVGLYMLGWGGDNGDPDNFTGYFFGGGAEPIKRE
                     GWYQNAELADLLYTALTTPDQAAREEMYKQAEQIMHDDVTRLWVAHNNTPLIFSAKVS
                     GYQAQPVGADYYEGVVVAP"
BASE COUNT          567 a          658 c          605 g          561 t
ORIGIN      
        1 tttcccgcag tctggttaat tttcctcaac aaacaggtcc aggcttggat ttgtacctgc
       61 ccagatacag cttgcccgcc catccagatt gcagctaaaa gcatgcgagc agcgatgggc
      121 taatatgcgg ggttgttgat cagggagaaa gtcagccgga taaacaacct gagcttcaaa
      181 tcctacattt ttatcaacat ggtggcagta ccgaacacgg agaacttccc aagtctttgt
      241 ggccataccc tacctcctca tcttgcttga ttatacccac ccgatcagcc ctgcatagtg
      301 tcaggtgtca tattataaac tggacaaatt gcgtcattta tgggcacaac aattaagcag
      361 aaatgaatct gttgatccca ggcaagccct accaattgac cagtcctaaa ggcagagcca
      421 ttcaattcta aatatggtcg ttgacgggaa gtaaagctta tgtataatag actaactctt
      481 tttccgccca atttcgtgga ggtgtaaatg aaataatacc ttgcggaacg tcccattcta
      541 tccaacaaat caagaatcga ttttctctca ggaggagaaa aagcatgagc cgtaaacact
      601 tattgacctt ggggtcaatc ctgatcgtct tcagtatgat cctgagcgcc tgcgcccagg
      661 ctacccctgc gccgaccgaa gcgcctgccg aacctgtggc aaccgaagcc ccggccgaac
      721 cggtcgcgac tgaagcgccc gctgagccca tggaagaatc tgtattcatc ttcgggcgtg
      781 gcgccgattc tatttccctg gatcccgccc tcgtcaccga tggtgaaagc ttccgcgtta
      841 ctgggcagtg cctcgaacca ctctaccagt acgagcctgg aaccaccaag ccgatccctg
      901 ctttggcgac cgcctgcacc cccaatgacg acgcaatgga atggacctgc aatttacgtg
      961 aaggcgtcaa attccatgat ggcaccgatt tcaatgctga tgccgtgatc tttaactatg
     1021 agcgctggcg ctttacggac aaccccctcc acttcgaatc ccaggttttc gaatattatg
     1081 aagccatgtg gggcgggttc gatgatgcca gcctgatcac atcggtcgaa aaggtggatg
     1141 acctgacggt gaaatttgtc ctcagcaccc ccatggcgcc cttcctggct aacctggcca
     1201 tggatatgtt cgctatctcc agcccggcgg ccatggaagc tgccggtgaa aactatggca
     1261 ctcccagcgt gggttgtgtc ggcactggcc cctataaatt cgtagaatgg gtggaaggcg
     1321 attcaatcac cgttgaagcc aatgccgact attggggcgg agagccaccg accaagaaga
     1381 ttatctggcg tgtgatcccc gatgactcag ctcgtttcct ggcgcttaag gctggcgata
     1441 ttcagggcct ggaacaagcg actgtagaag acttgagcag cgcagagaac gaccctgagc
     1501 tccagattat tacccgcccc gcattgaaca ctgcctacct ggctttcaac tataaagttg
     1561 tagaattcca ggatatccgc gttcgcgaag ctgtggcgca tgctgtcaac cgcgccgggt
     1621 tggtcgagaa cttctatggc gcatatggcg aagttgccaa gaatctactg cctcctctgg
     1681 tttggggtca caatgacgcc atcgaagact ggacttatga tcctgagctt tctaagtccc
     1741 tcctggccga agccggcttc ccggatggtt tgagcgaagt gacgattgct gaagacatca
     1801 atgatgccga aggcaatcca atctttaccg ccggtgagaa aattccgtta cgcctgtact
     1861 acatgccagc cacccgcttc tattatccaa gcccgaaaga gatcggcgag gctatggcgg
     1921 ctgatttagt tgctgctggc atcaacgccg agctttacct ggaaggcgac tgggcgactt
     1981 acctcggcgc tcgcagcaac ggcgcgctgg ttggcctgta catgctgggt tggggcggtg
     2041 ataacggcga tcccgacaac tttaccggtt acttcttcgg cggcggcgct gagccaatca
     2101 aacgagaagg ctggtatcag aatgcagaat tggctgactt gctgtatacc gctctcacca
     2161 cccctgatca ggctgcccgc gaggaaatgt acaagcaggc cgagcagatc atgcatgacg
     2221 atgttacccg tctatgggta gcccacaaca atactccttt gatcttctcc gcaaaggtca
     2281 gcggctacca ggctcagccg gttggtgcag actactatga aggtgtggtc gttgctccct
     2341 aaggtctgga aagctcgtta cgtacgtcgg tttttcaact aatcactctc t
//
LOCUS       DATMXK010000372         3085 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_140192, whole genome shotgun sequence.
ACCESSION   DATMXK010000372 DATMXK010000000
VERSION     DATMXK010000372.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3085)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3085)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3085
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140192"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1107)
                     /locus_tag="VN363_06910"
     CDS             complement(<1..1107)
                     /locus_tag="VN363_06910"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ84278.1"
                     /translation="MSIETVAITQPTEDEFKTSQVLTIVGGHFFHDLYTAFLAPLLPL
                     LIEKLSISLTLAGSLTALTQISGLLNPFIGYLADKVSLRYFVIFAPGITATLISSMGF
                     APSYFALAIILFVTGISVAAFHAPAPAMIGEVSGKKVGLGMSLFMAGGELGRSAGPLL
                     AVWAVSTWTLDGYYRIMLLGWAATVVLYLRLHNLQAQPTQNGSLRAILPLFKTLFLPL
                     VFITLFRDFLISGISVYLPTFMNGEGASLWMAGSALSIYELAGVGGALTSGTLSDRFG
                     RKLLLVLSAVFSTLFVFFFLNVQGWAQAVALLLLGFVNLSSTPVLLALVQEQLPGNRA
                     VGNGLFMAINFLALLLTTLGVGLIGDHFGLRAAFN"
     gene            complement(1178..2632)
                     /gene="arsM"
                     /locus_tag="VN363_06915"
     CDS             complement(1178..2632)
                     /gene="arsM"
                     /locus_tag="VN363_06915"
                     /inference="COORDINATES: protein motif:HMM:NF008823.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="arsenite methyltransferase"
                     /protein_id="HWQ84279.1"
                     /translation="MSMTAASYEYFNKVAGEWDAIRSGYFSEAVREAAIRKAYLRPEM
                     IVGDIGAGTGYVSAGLAPLVRQVYVLDGSQAMLEVARQNLHEFDNVIFETADGSSLPL
                     PDASLDAVFANMYLHHLPEPLAGIREAARLLKPGGRLVITEMDAHSYGWLKEEMADVW
                     QGFDREQMRNWYEAAGLVNIIIESTGQSCSATSEKTSITDAEGRSADISVFVAAGTKR
                     VSGALQAVRESYGAAALNSSGCCSPESSSQSSSACCGGSAMDVIPLSSLEVSFNPNYS
                     LHETKSVPSEAASLALGCGNPTGMAGVQAGETVLDIGSGAGMDAFLAARQVGPAGKVI
                     GVDMTPAMLERARRGAAKSGLTQVEFRQGQAEALPVEDNSVDVIISNCVINLTEDKGL
                     VFREAYRVLKPGGRLEVSDMVTDTSIPAELRSDPNNWSGCVFGALPEQEYLDLVAQAG
                     FQEIRTQQSLSYGENESVTLYSLAVSARKLPLPG"
     gene            complement(2695..3039)
                     /locus_tag="VN363_06920"
     CDS             complement(2695..3039)
                     /locus_tag="VN363_06920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560480.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWQ84280.1"
                     /translation="MDHRQSHVRLGKMLKALGNPVRFQIMETLAERQSCITNEIVETT
                     SLAQSTVSQHLKVLREAGLIHGEIEGPATCYCVDSDGIRFLKDQIESWLPDCCSPVQI
                     TLPSEQQSDTCC"
BASE COUNT          746 a          867 c          789 g          683 t
ORIGIN      
        1 gttgaatgcc gcccgcaggc caaaatgatc gccgatcaaa cctacgccga gggtggtcag
       61 aagtaacgcc aggaaattaa ttgccatgaa taaaccgttg ccaaccgccc ggtttccggg
      121 gagctgttcc tggacgaggg ccagtaatac cggcgtggaa gataaattaa caaaacccag
      181 caaaagtaat gctaccgcct gcgcccagcc ctggacattg agaaagaaaa atacaaacaa
      241 ggtagaaaac actgccgaca aaaccaggag taattttcgt ccaaaccggt cgctcaatgt
      301 tccactggtc agcgcgccgc ccacacctgc cagctcgtag atggatagtg cgctgccggc
      361 catccacagg ctggcgcctt ccccgttcat gaaagttggc agatagactg aaatccctga
      421 gatcagaaaa tctctgaaaa gcgtgatgaa gaccaggggc aggaataatg ttttgaataa
      481 cggcaggatc gcccgcaggc tgccgttttg ggttggctgg gcctggagat tgtgcaaacg
      541 caggtaaagg acaacggtag ccgcccaacc cagcagcata atccggtaat agccatccag
      601 cgtccaggtc gatacagccc agacagccag cagtggaccg gcactgcgcc ccagctcgcc
      661 gccagccatg aacaggctca ttcccaggcc cactttttta cctgagactt caccgatcat
      721 ggccggagca ggtgcatgga atgctgccac gctgatcccg gtgacgaaaa gtatgatcgc
      781 cagggcaaag taactgggag cgaagcccat gctgctgatc aaagttgctg ttatccctgg
      841 agcaaagata acaaaatatc gcaggctgac cttatcggcc agataaccga taaaaggatt
      901 gagcaagccg gaaatctggg tcagtgcggt gagtgatccc gccagggtga gagatataga
      961 gagtttttca attaagagag gtaataacgg tgcgagaaaa gcggtatata aatcgtggaa
     1021 gaaatgccca ccgacgatgg tcaaaacctg gctggtttta aactcatctt cagtgggctg
     1081 tgtgatcgcc acagtttcaa tgctcatgga tggggtcccc ggttcccatc gattgtaatc
     1141 gatatttcct tccagatcga accctgctgt tatggtgcta acccggtagg ggcaattttc
     1201 gggctgagac cgctaaacta tagagtgtaa cgctttcgtt ctcgccataa ctcagactct
     1261 gttgggtgcg gatctcttga aaaccagcct gtgctaccag atccaggtac tcttgctcag
     1321 gtaaagcacc gaatacacac cctgaccagt tattcggatc agaacgtaat tcggcaggaa
     1381 tagatgtgtc cgttaccata tcggatactt ccagccgccc gcctggtttc aacacccggt
     1441 aagcctcgcg aaataccagt cctttatcct ctgtgaggtt gatcacgcag ttggagatga
     1501 ttacatcaac agaattatct tcaaccggca gcgcttcggc ctggccctga cgaaattcca
     1561 cctgggtcag gccagatttc gccgccccac gccgggcgcg ctccagcatc gctggcgtca
     1621 tatccactcc gatcactttt cctgccggtc caacctgcct ggcagccagg aatgcatcca
     1681 ttccggctcc actcccgata tccagcacgg tttctcctgc ctgaacgcca gccatgccgg
     1741 tcggattccc gcaccctaaa gccagactgg ctgcctcgct ggggacggac ttcgtctcgt
     1801 gcaggctgta attgggattg aagctcacct ccaggctgct gagcgggatc acatccatcg
     1861 cgctgccgcc gcagcaggcg ctgctgcttt gagaactact ttcaggggag cagcaccccg
     1921 agctgttcag ggcggctgca ccgtagcttt ccctcactgc ctgcagggcg ccacttaccc
     1981 gtttcgtgcc agccgccaca aatacgctaa tatccgcact cctgccttct gcgtcagtga
     2041 ttgaagtctt ctcggaggtt gctgagcatg attgaccggt cgactcgata atgatattaa
     2101 ccaagccagc cgcttcatac cagtttcgca tttgttcacg atcgaacccc tgccagacgt
     2161 ccgccatctc ttctttcagc caaccgtagg agtgagcatc catctcggta attaccagcc
     2221 gaccgccggg tttgagcaat cgcgctgctt ctcggatgcc tgccagtggt tcgggcagat
     2281 ggtgcaggta catattggcg aatacagcgt ccaggctcgc atccgggaga ggcagagaag
     2341 agccatcggc agtttcaaag ataacgttat caaattcatg caggttttgg cgagccacct
     2401 ccagcatggc ttgggatcca tccagcacat atacctgccg gactaagggc gccaggccag
     2461 cgctcacgta acctgttcca gctccgatat cgccaacaat catttctggg cgcaggtagg
     2521 ccttgcggat cgccgcctcg cgaactgcct cactgaaata accagagcgg attgcatccc
     2581 actcaccggc gactttatta aaatactcgt acgatgcggc agtcatactc attgatgtct
     2641 cctaattcat cgcaaaatta cgatattgtt ggacaaaaag aatggattcg cgaattaaca
     2701 gcaggtatca gactgttgtt cggacggtaa agtgatttgg accggcgagc agcaatctgg
     2761 cagccaactt tcgatctgat ctttcaggaa gcggatgcca tcggagtcta cgcagtagca
     2821 ggttgcaggg ccctcgatct caccgtggat caaaccggcc tcacgcagca cctttagatg
     2881 ctggctgaca gttgattggg ccagggaggt tgtctcgacg atctcattgg tgatacaact
     2941 ctggcgttct gcaagggttt ccatgatctg gaaacgaacc ggattcccca gggctttaag
     3001 catcttgccc agccggacat ggctctgccg atgatccatt ctgtttgctg gtatttccat
     3061 acaactatac tccattccta tatcg
//
LOCUS       DATMXK010000373         4793 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_1428, whole genome shotgun sequence.
ACCESSION   DATMXK010000373 DATMXK010000000
VERSION     DATMXK010000373.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4793)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4793)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4793
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1428"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..762)
                     /locus_tag="VN363_06925"
     CDS             complement(37..762)
                     /locus_tag="VN363_06925"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWQ84281.1"
                     /translation="MQLYFIRHGQSMNNQLWEQTQASKGRSMDPELTDKGWQQASRLA
                     SFLRENQLPLPTESFNTLNRYGFGLTHLYSSLMVRAVATGSSVARALNMPLVGWLDLH
                     EEGGIYMDDPETGEPLGYPGKDQAYFGEHYPELVLKDGAALKGWWNRPYETSAERLAR
                     VKRFHTELISKHGDTDDHVAIFSHGGFYNLFLRYVLGLPVDSNSLWFVLNNTAISRID
                     FVAGETFVAYLNRVDFLPVDLVT"
     gene            924..3155
                     /locus_tag="VN363_06930"
     CDS             924..3155
                     /locus_tag="VN363_06930"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559861.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine dehydrogenase family protein
                     molybdopterin-binding subunit"
                     /protein_id="HWQ84282.1"
                     /translation="MPSIGQSVQRIDALSKVTGQACYPGDINLPNQAYMKILFANRPH
                     AIIHKIDTAAAEELPGVLAVFTARDVPVNEYGLITSDQPVLCGPGSSKPFAERVRFVG
                     DQVALVIAETEELAARARDLIQVDYEDLPVVTDPIQARLPSAELLHPDRDSNIFCHYR
                     IRKGDVDTTFAQADVIIEGEYHTPAQEHAYLQPEAGISYIDEEGRVTVEVAGQWTHED
                     QEQIAHALGLPLEQVRVIYPAIGGAFGGREDMSVQIVLGLAAYRLHERGINRPVKIIW
                     SREESIIGHHKRHPYILKTRWGATKAGKVIAAETEVIADGGAYIYTSTKVLGNATLMC
                     TGPYEIPNVKVDSYAIYTNNIPGGAFRGFGGPQGAFAAEMQMNRLAEKLGLDPVEIRL
                     RNVLREGSLLSVGTPLPEGVSLPEVVAECARTSGWQEQPDARWTPPAVPTGLFNLTTP
                     HPHLKRGVGFACAFKNVGFSYGAPENCWAIIELYGKGEIDQVVLKHAGAEVGQGSHTV
                     FCQMAADAVGVPLEKVTLVASDTAVTRNSGSVSASRMTFMAGNAIRGAASQALEHWKA
                     EDRPAQGIFQYRPPATTPLDPETGKSEPNFAYGYVAQSVLVEVDIETGHVNLIDVVST
                     NDVGKAINPQLVQGQIEGGVVQAAGYAVLENFVQKDGYVQTQHLSTYLIPTVLDIPEQ
                     VHSQILEISDPRGPWGARGMAEMPFLPLAPAVISAVHDATGVWIHEFPLTPERVLHAL
                     GRI"
     gene            3172..3990
                     /gene="yqeB"
                     /locus_tag="VN363_06935"
     CDS             3172..3990
                     /gene="yqeB"
                     /locus_tag="VN363_06935"
                     /inference="COORDINATES: protein motif:HMM:TIGR03309.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium-dependent molybdenum cofactor
                     biosynthesis protein YqeB"
                     /protein_id="HWQ84283.1"
                     /translation="MDDGQPILILRGGGDLASGVALRCFRAGFHILITEIPQPLAVRR
                     TVAFAEAIYMGSIQVEEATGRKVNTPEEIQAAWQTDEIPVIADPECAIRHVLHPTILV
                     DGRMTKQPPDLGRETAGLVIGLGPGFTAGINCHAVIETNRGHFLGRVLWDGPAQADTG
                     IPEGVDHHQEDRVLRAPAAGRVTLFVTIGEIVEPGEAIAQVQEQIIYAPFKGLVRGLI
                     HPGIQVQIGTKIGDLDPRCDPRYTQFVSEKALAVGGGVLEAALSRPDIRHRLLG"
     gene            3992..>4793
                     /gene="yqeC"
                     /locus_tag="VN363_06940"
     CDS             3992..>4793
                     /gene="yqeC"
                     /locus_tag="VN363_06940"
                     /inference="COORDINATES: protein motif:HMM:TIGR03172.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="selenium cofactor biosynthesis protein YqeC"
                     /protein_id="HWQ84284.1"
                     /translation="MNLADALLLENTSRLALVGAGGKTSAMFAAAKAFIQRRQAPRPI
                     LLTTTTHLAIEQCSLADHHFKIHSAEDVNARVSELNSGILLFTGTEEKDGRISGPDPQ
                     ALEAIHTLADGLDCPLLVEADGSRLHPLKAPADFEPVIPEWVNHVLVCAGLAGLGEPL
                     DAEIVHRPEIYAQLSGLVPGAPITPQAIVRVLRHSSGGLKAIPCHARRSVLLNQADTP
                     EKQAAARRMADQLLGDYERVIISSLNLAVGDPVIHAAITPAAGIVLAAG"
BASE COUNT         1122 a         1327 c         1317 g         1027 t
ORIGIN      
        1 tatctggctg aaaaaccagc tgttcgtcat ccagaattaa gtaactaaat ccacggggag
       61 aaaatctacc cggttaagat aggccacaaa agtttcaccg gcgacaaaat cgatccggct
      121 gatggcggtg ttattgagca caaaccacag gctgttcgag tctaccggta gacccaacac
      181 ataccgcagg aagagattat aaaatcctcc atggctgaaa atagccacat gatcgtcggt
      241 atcaccatgt ttagaaataa gctctgtatg aaaacgcttt acacgggcga ggcgttcggc
      301 agaagtctca taagggcggt tccaccagcc ttttagcgca gcaccatctt tgagaacgag
      361 ctcgggataa tgttccccaa aatacgcctg gtctttccct ggatatccca agggttcacc
      421 ggtttctgga tcgtccatat agatgccgcc ctcttcatgt aagtccagcc agcctaccag
      481 gggcatattt aaagcgcgag ccacgctgct gccagttgcg accgccctga ccatcagact
      541 ggaatataaa tgagtcaatc caaatccata cctgttcagg gtattgaaag attcggttgg
      601 cagcggcagc tgattctcac gtaaaaacga tgccagccgg cttgcctgct gccagccttt
      661 atcggtcagc tcgggatcca tactgcgtcc tttgctggcc tgggtttgct cccaaagctg
      721 gttattcatc gattgaccat ggcgaatgaa gtagagctgc atttagtgac tcgtttccat
      781 cggatgtagt ttaagcgaga cagaatatac cccaggcttc cagatcagac aagagtatcc
      841 agcaatcgag tctatctcac ccgagaggta taatttacca gagtcaggct gactgaactc
      901 attatagaac caggagaaat gatatgccat ctatcggtca atcggtccag cgcattgatg
      961 ccctcagcaa agtaaccgga caggcctgct accctggaga tattaacctg cccaaccagg
     1021 cttatatgaa gatcctgttt gccaaccgtc cacatgcgat catccataaa atcgatacag
     1081 ctgccgctga agaactaccc ggtgtgctgg cggttttcac cgccagagat gtcccggtta
     1141 atgagtatgg gctgatcaca tctgaccaac ccgtattgtg cggcccgggc tccagcaaac
     1201 cctttgccga gcgggtgcga tttgtcggcg accaggtagc cctggtcatc gccgaaactg
     1261 aagagcttgc agcccgagcc cgcgacctga ttcaggtcga ttatgaagat ttaccggtcg
     1321 taaccgatcc gattcaggct cgcctgccga gcgcggagtt actccatccc gaccgcgatt
     1381 caaatatatt ttgccattac cgcattcgta aaggcgatgt cgatacaaca ttcgcccagg
     1441 cagatgtcat catcgaagga gaatatcaca ctccagccca ggagcacgcc tacctgcagc
     1501 ctgaagccgg gatcagctat atcgatgaag aaggccgggt gacagttgag gtcgccggac
     1561 aatggactca cgaagaccag gaacaaatcg cccatgcttt aggcttacct ctggaacagg
     1621 tgcgggtcat ttatcccgcg atcggcggcg cctttggagg gcgagaagac atgtcggtcc
     1681 agattgtatt gggtctggcg gcataccggc tgcatgaacg cggtataaac cgtccggtaa
     1741 agattatctg gagccgggaa gagtcgatta tcgggcatca caaacgccat ccctatatcc
     1801 tgaaaacacg ctggggggcc acaaaagccg gtaaagtcat cgctgctgag actgaagtga
     1861 ttgcggatgg tggggcatat atctatacct cgacgaaagt cctgggtaac gcgacgttga
     1921 tgtgtaccgg tccatacgaa atcccgaatg ttaaagtcga cagctatgcg atctacacca
     1981 ataacatccc gggcggcgca tttcgcggct ttggtggtcc gcaaggagct tttgctgcgg
     2041 aaatgcagat gaaccggctg gctgaaaaac ttggcctcga tccggtagaa atccgcttgc
     2101 gcaacgtgct gcgggaagga tccctgcttt cggtcggtac tccccttccc gaaggggtca
     2161 gcctgccaga agtggtggcc gaatgcgccc gtaccagcgg ctggcaagag cagccagatg
     2221 cccgctggac acctccggcg gtccccactg gattatttaa ccttacaacc ccacatcccc
     2281 accttaagcg tggggttgga tttgcctgtg cctttaagaa cgtgggcttt tcttatggag
     2341 cacctgaaaa ctgctgggcg atcatcgagc tgtatggcaa aggcgaaatc gatcaggtgg
     2401 tgctcaagca tgctggcgca gaagtcggcc agggctcgca tacagttttc tgccagatgg
     2461 cagccgatgc cgtcggcgtg ccattagaga aagtcactct ggttgcctcg gatactgccg
     2521 tcaccagaaa ttcaggcagt gtttcggctt caaggatgac tttcatggca ggcaatgcca
     2581 ttcggggtgc agccagccag gctttagaac actggaaggc tgaagatcgc ccggcacagg
     2641 gtattttcca gtatcgtccg ccggcgacca cgccgcttga tccggaaacc ggcaaatccg
     2701 agccaaattt tgcctacggg tatgtcgccc agagcgtcct ggtcgaagta gatatcgaga
     2761 ctggccatgt caatctgatt gacgtcgttt caacgaacga tgtaggtaag gcaatcaatc
     2821 ctcagctcgt tcaggggcaa attgaaggcg gggtcgtaca ggcagccggc tatgcagttc
     2881 tggaaaattt cgtccagaag gatggatatg tacagacaca gcacctgtcc acctacctga
     2941 tcccgacggt actggacatt ccggaacagg tgcacagcca gatcctggaa atcagcgacc
     3001 cacgtgggcc ctggggtgcg cgcggtatgg ctgaaatgcc attcctgccg ttggcgccag
     3061 cggtgatttc agcagtccat gatgcaaccg gagtctggat ccacgaattt cccctgaccc
     3121 cggagcgagt attacacgcc ctgggacgga tctaaattac ggtcctcgct catggatgat
     3181 ggtcagccaa ttttgatcct gcggggcggt ggagacctgg cttctggggt tgccttacgc
     3241 tgcttccgcg ccggatttca catcctgatc actgaaattc cacagcctct ggccgtccgg
     3301 cgcacagtgg ccttcgcaga ggcaatatac atgggttcga ttcaggtgga agaagctacc
     3361 ggtcggaaag taaatacacc tgaggaaatt caggcggcat ggcagactga cgagattcct
     3421 gtaattgcgg atccggagtg cgcaatccgc cacgtgctcc accctaccat tctggtcgat
     3481 gggcggatga ccaagcagcc ccctgatctc ggccgcgaga ctgccgggct ggtcatcggg
     3541 ctgggaccgg gattcactgc cggaataaac tgccatgcag tgatcgaaac gaaccgggga
     3601 cacttcttag gccgcgtcct ctgggatgga ccagctcagg cagataccgg tatcccggaa
     3661 ggcgtggacc accatcagga agatcgcgtc ctgcgtgccc cggcagcagg cagggtcact
     3721 ctatttgtga ccataggcga gattgtcgag cccggggagg caatcgctca ggtccaggag
     3781 cagattatct acgctccctt caaaggattg gtccgcggac tgatccaccc gggcattcag
     3841 gttcaaatcg ggacgaaaat cggagacctg gatccccgct gtgacccacg ctacactcaa
     3901 ttcgtttcag aaaaagccct ggcggtcggg ggtggcgtgc tggaagcagc tctcagccgc
     3961 cctgatatcc ggcacaggct gctgggctga tatgaacctt gccgacgccc tcctcctgga
     4021 gaataccagc cgtctggccc tggttggcgc agggggtaaa accagcgcca tgttcgccgc
     4081 tgcaaaggct ttcattcaac gcaggcaggc gcctcgtccg atcctgctca caacaaccac
     4141 ccatctggca atcgaacaat gcagcctggc tgatcatcac tttaagatcc attctgcaga
     4201 ggatgtcaat gcccgtgtaa gtgagctaaa ctctggaatc ttattattta cgggcacaga
     4261 ggaaaaagac gggcgtatct ctggaccgga tccacaggca ttggaagcaa tccacaccct
     4321 ggcagatggt ttggactgtc cactgttggt tgaggcggat ggctcgcgtt tacatcccct
     4381 gaaagctcct gcagatttcg agccagtcat cccggaatgg gtcaaccacg tactggtatg
     4441 cgccggtctg gctgggttgg gcgaacccct ggatgcagaa atcgtccacc ggcctgaaat
     4501 ctatgcgcag ttatctgggc tggtcccggg tgcccccatt acaccccagg caattgtgcg
     4561 tgtcctgcgc cacagctcgg gtgggttgaa ggcgatcccc tgccatgccc ggcgcagtgt
     4621 cctgctcaac caggcggata ctcccgagaa acaggccgcc gcccggcgga tggcagatca
     4681 actcctggga gattatgagc gagtaattat cagcagtctg aatcttgcgg ttggtgatcc
     4741 agttatccat gcagccatca caccggcagc cggaatcgtc ctggcagccg gcc
//
LOCUS       DATMXK010000374         2855 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_71806, whole genome shotgun sequence.
ACCESSION   DATMXK010000374 DATMXK010000000
VERSION     DATMXK010000374.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2855)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2855)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2855
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_71806"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..568
                     /locus_tag="VN363_06945"
     CDS             <1..568
                     /locus_tag="VN363_06945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558531.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ84285.1"
                     /translation="EYAAQNPQIQVIREEARGKGLAVRSGMLTARGEYRFMADADFSM
                     PPQYINRFLPPDLPKCDIAIASREAPGAVRYGEPAYRHFIGRIFNLMIRTLALPEFED
                     TQCGFKCLRGEVAEEVFPYQTVSGWAFDVEILFIARQRGYRIVELPIPWYFNEDSKVR
                     VIRDSLEMARDLLRIRWNSLRGRYNERR"
     gene            555..1202
                     /locus_tag="VN363_06950"
     CDS             555..1202
                     /locus_tag="VN363_06950"
                     /EC_number="3.1.26.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558532.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003723 - RNA binding [Evidence IEA];
                     GO_function: GO:0004523 - RNA-DNA hybrid ribonuclease
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribonuclease HII"
                     /protein_id="HWQ84286.1"
                     /translation="MSGAKASLPTAPSLDFERTLWRAGYQFIAGVDEAGRGALAGPVA
                     AGAVILPCRADLGAILDGVRDSKLMSAAARQLWRDVICRTAVSWAVGFASQLEVDCLG
                     ILPATRLAASRAIDLLSIAPDYLLTDYLILSEEEAPQTGLVKGDRLVLSIAAASILAK
                     TSRDQWMTEAGRAYPGYGFAQHKGYGTRQHRQALFRLGNTPLHRLSFHYSCADLD"
     gene            1228..>2855
                     /locus_tag="VN363_06955"
     CDS             1228..>2855
                     /locus_tag="VN363_06955"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolyl oligopeptidase family serine peptidase"
                     /protein_id="HWQ84287.1"
                     /translation="MPETINHKSFGLWKSPLTPKRMSGSLRFSDLGWDEAGNLVWSES
                     RLGRDTLVVQPSDGQAPRDINNDYTVRAGVGYGGGDFSVSAGQVFFVDAETGGLFRQS
                     LNQGQARAVTPAFGACAAPVLSPDENWLLYVHSYQNIDCLALTDVEGKFWPQRIVSGR
                     DFYMQPVWRPNPSGGSLWIAWVQWSHPNMPWDGTELCMGRLETAEESLPVLAETRVVA
                     GDVHTSIFQPEFSPDGKYLAYVSDISGWWQLYLYDLETGKTIQLTSASAEHAVPAWNQ
                     GQRTYQFAPDGENIYCLRNQAGRVTLWQIEIATVTERQVILDVSYGSLEQIAIRRGHD
                     HPLEIALIASDDRIPPRIILCRPEQGITRVIRRSAAEDLPIETYSLSRPVQWTGMDGG
                     VVYGLFYPPQNPGFRGNGLPPLIVKIHGGPTSQQRATFIPDDQFFTSRGYAVLEVNYR
                     GSTGYGRAYAQALRANWGIFDVQDAVSGVNALVELGLVDRDKVIIKGGSSGGYTVLKA
                     LIDFPGFFKAGICLYGISNHFAISAETHKFEAHY"
BASE COUNT          622 a          714 c          825 g          694 t
ORIGIN      
        1 cgaatatgcc gcgcagaatc cccaaataca ggtgatccgg gaagaggcgc gtggaaaagg
       61 tctggcagtc cgctctggta tgcttacggc acgcggagaa taccgcttca tggctgatgc
      121 ggatttttca atgcctccgc aatacattaa ccgttttctt ccacctgatc tcccaaagtg
      181 tgatatcgcg attgcatcgc gtgaagcgcc tggagctgtc cggtacggcg agccggctta
      241 ccgtcacttt attgggcgga tatttaacct tatgattcgc actctggctt tgcctgaatt
      301 cgaggatact caatgtggct tcaaatgctt acgcggggaa gttgctgaag aggtattccc
      361 ctatcagact gtatccggat gggcatttga tgtggaaatt ctatttatcg ctcgccaacg
      421 aggttaccgg atcgtggaat taccgatccc ctggtacttt aacgaggata gtaaggttag
      481 ggtgatccgc gattctcttg agatggcgcg ggatctattg cgcattcgct ggaactcact
      541 gcgaggtcgt tacaatgagc ggcgctaagg cttccttacc aacagctccc agcctggatt
      601 ttgaacgaac attgtggaga gcaggctacc agtttattgc cggggttgac gaggcaggca
      661 ggggggcgct ggctgggccg gtcgccgccg gtgctgtgat cttaccctgc cgggctgacc
      721 tgggtgcaat tctggatggt gtccgagatt cgaagctgat gagtgctgcg gcgcggcagc
      781 tctggcgaga tgtgatctgc cggactgctg taagctgggc ggtcggcttt gccagccaac
      841 tggaagtaga ttgtttaggc atcctaccag ccactcgcct ggcagcctcc cgggcaatcg
      901 acctgctgtc gatagccccc gattacctgc taaccgatta tctgatcctg tcagaggaag
      961 aggcgcctca gactggtctg gtgaaggggg atcgtctggt cttaagtatt gctgcggcat
     1021 cgatcctggc taaaacctcc cgggatcagt ggatgaccga ggcaggcaga gcatatcccg
     1081 ggtatggctt tgcgcagcac aaaggctatg ggacccgcca acaccgccag gcgctgttta
     1141 gactcggaaa tacgcctctg caccggctca gcttccacta ctcttgtgct gacctggatt
     1201 agagaattct tgcggtgagg ttggtcgatg cctgaaacca taaaccataa gtcttttggt
     1261 ctctggaaaa gcccactgac cccgaaacgg atgtctggat ccttaagatt ttctgatctg
     1321 ggctgggatg aggcgggcaa tcttgtctgg tcggaaagcc ggttgggtcg cgataccctg
     1381 gtcgtacagc cctccgatgg acaggcacct cgggatatta acaacgacta cactgtacgc
     1441 gcaggtgtag gttacggcgg aggtgatttt agtgtttccg cagggcaggt gttctttgtc
     1501 gatgcagaaa ctggtgggct attccgccaa tcgctcaatc aaggacaggc cagggcggtt
     1561 accccagcat ttggcgcatg cgcagcaccg gtgctctcac cggatgaaaa ttggcttctc
     1621 tacgttcata gctaccagaa tatagactgc ctggctttga ccgatgtgga aggcaaattc
     1681 tggcctcagc ggattgtaag tggtcgggat ttctatatgc agccagtctg gcgacccaat
     1741 ccctcaggag gttccctgtg gattgcctgg gtacagtgga gccatcccaa catgccctgg
     1801 gatgggacag agctgtgtat ggggcgcctg gaaacagccg aagagagttt accagtgctg
     1861 gcagaaacca gagtcgtagc aggtgatgtg cacacatcga ttttccagcc agaattttca
     1921 ccagacggta aatatttagc ttatgtatcg gatataagcg ggtggtggca gctctatctt
     1981 tatgatctgg agactggaaa aacaatccag ctgacctcag cctcagcgga gcatgccgtc
     2041 cctgcctgga accaggggca gcgcacttac cagttcgcgc ccgatggtga aaatatttac
     2101 tgcttgcgaa atcaagcggg tcgcgtcact ctgtggcaaa ttgagattgc cacggttaca
     2161 gagcgccagg tgatattgga tgtttcatac ggcagcctgg aacaaatcgc gatccggagg
     2221 ggacacgatc atccactgga gattgctctt atcgcctccg atgaccgtat tccaccccgg
     2281 attattcttt gccgtccgga gcaaggaatc acacgcgtca tacggcgttc agcagctgaa
     2341 gatcttccga tcgagacata ctccctgtct cgaccggtac aatggaccgg tatggatggg
     2401 ggagtcgttt acgggttgtt ttatccccct caaaatccgg gttttcgtgg aaatggtttg
     2461 ccgccattaa ttgtaaaaat tcacggcgga ccgaccagcc agcagcgtgc gacttttatc
     2521 ccagacgatc aatttttcac cagccgcggc tacgcagtgc tggaggtcaa ttaccggggg
     2581 agtacaggat atggacgggc gtatgcccag gctttgcggg caaattgggg gatttttgac
     2641 gtccaggatg ctgtcagtgg agtgaatgcc ctggtggagc ttggtcttgt tgaccgggat
     2701 aaggtgatca taaagggggg gagctccggg ggttataccg tcctgaaggc gctcatagat
     2761 ttccccggat tcttcaaagc cggcatctgt ctttacggga tctccaatca ctttgccatt
     2821 tctgctgaga cacataaatt tgaagcccat tataa
//
LOCUS       DATMXK010000375         4491 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_103130, whole genome shotgun sequence.
ACCESSION   DATMXK010000375 DATMXK010000000
VERSION     DATMXK010000375.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4491)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4491)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4491
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_103130"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..192)
                     /locus_tag="VN363_06960"
     CDS             complement(<1..192)
                     /locus_tag="VN363_06960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84288.1"
                     /translation="MAKLKYDGVIEAVHYRPDGKVDWARTYLRRGPTFSDYILLDRQA
                     LLEHIHAGEKFYIGQRIPLK"
     gene            complement(262..335)
                     /locus_tag="VN363_06965"
     tRNA            complement(262..335)
                     /locus_tag="VN363_06965"
                     /product="tRNA-Pro"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(299..301),aa:Pro,seq:tgg)
     gene            391..1137
                     /locus_tag="VN363_06970"
     CDS             391..1137
                     /locus_tag="VN363_06970"
                     /EC_number="2.1.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025217.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ84289.1"
                     /translation="MNSSLKLAYLYAAHTQRHLEDLPFWLGLAHQADGPILELGCGTG
                     RILLSVLQSGLPIIGLDRDPDMLAVLKEKSCKAQKSPAIVQADMSAFNFEQLFSLIIL
                     PCNTISTLSDSSRVTMLNAVVDHLAEGGIFATSLPNPLLLSNLPEHAEPEIEESFPHP
                     LDGAPVKVSSSWEHDQHTFSILWDYEYGLPSGDIEHLTLTSSHGLIPISQYLAEFETA
                     GLNILHQYGDFNKRLFNRRANSLIFVAQKP"
     gene            complement(1262..2362)
                     /locus_tag="VN363_06975"
     CDS             complement(1262..2362)
                     /locus_tag="VN363_06975"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84290.1"
                     /translation="MLTDRDLQELLSYQAKSPILSVYQNTDPAESSTDGHRLHLRSML
                     KGVNQPDDVAAVLRYFDHEHNWTGRSVAIFSCSAEGFFRAYPLHVRLRPRVRTGDHPY
                     VKPLADVLDAFGGYGVVVVDKQGARLFKFHLGELSEQEGVMGETIRHAKRGGGSQAAG
                     RRSGNAKESDYVDELTDRNIKDAVDFAVQFFTENNVRRILIGGTDDNVAAFRSHLSKA
                     WQSLVVGTFPVSMAATEPEILERAMKVGDAAELKREKKLVDAMITGASKGRGGTLQLE
                     DTLGAVRSGRVQTLILRDGFRAPGYQCLGCEYVTVEKLDLCPFCGGVFKQLPDAVEEA
                     VRQVLAQGGDVEVLHDPQLVGSFENIGALLRY"
     gene            complement(2431..3627)
                     /locus_tag="VN363_06980"
     CDS             complement(2431..3627)
                     /locus_tag="VN363_06980"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWQ84291.1"
                     /translation="MKRTSLIILAILVLALSGLACQTVTALPTSQTEVESPSTDATNP
                     PVAVVPAVTEASEAQVTVLDMISAQDTLIGLYQRVNPGVVSIRVLTEEGGGLGSGFVI
                     DTQGHVVTNYHVIEGVTDLEVAFASGFKARGEVIGEDLDSDLAIIKVDAPESELFPLP
                     LGDSDAVQVGQTVIAIGNPFGLAGTMTTGIVSGTGRTLESLHTTSDGGTFSAGDIIQT
                     DAAINPGNSGGPLLNLQGEVVGVNESIRTSNFTTTGEPTNSGIGFAVSINIVKKVAPS
                     LIADGKYDYPYLGITSLDDLTLVYQEALDLPTAAGVYVTGVTPGGPADRAGVRSGNLP
                     SDITGLPSGGDLITAIDDEPVTNFNDLISYLVKNKGPGETVELTIFRGSDEIKLEVTL
                     DKRPNP"
     gene            complement(3675..>4491)
                     /locus_tag="VN363_06985"
     CDS             complement(3675..>4491)
                     /locus_tag="VN363_06985"
                     /inference="COORDINATES: protein motif:HMM:NF013705.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="DnaJ C-terminal domain-containing protein"
                     /protein_id="HWQ84292.1"
                     /translation="DKTAEDKFKELNEAYQVLSDAEKKARYDQLGDSYFRYQQHGGSA
                     GNFNWEDWFTRSNANPGGNVRVEYGDINDVLGGEFSEFFTRIFGGMPGQQPRSTGRAS
                     PRPQPSYEQQVEISLQEVFSGATRLMEIDGRRLEVKIPKGARTGTKVRVPQVVSPGAS
                     GPKSDLYLVIQVASDPRFERKGDDLYTDVSIDVYTAVLGGDTQVDTFSGKVVLTIPPG
                     TQNNQTFRLAGRGLPTLKQPDRLGDLYVRIKVSIPRNLSDKQRSLFQELAQIK"
BASE COUNT         1091 a         1255 c         1054 g         1091 t
ORIGIN      
        1 ttttagagga atacgctggc caatataaaa tttttccccg gcatggatat gctcgagcaa
       61 agcctgccgg tccaggagga tataatcaga gaaagtgggc ccgcgccgta gataagtccg
      121 cgcccaatcc actttaccgt ccggccggta atggacagct tcgatgaccc catcatattt
      181 cagttttgcc attcttgctc tccgggacaa attattaagc ctgacataga gaagtcagat
      241 tcatatgcca ggctttccag gtcggggcgg gcggactcga accgccgacc cccgcgtccc
      301 aaacgcggtg cgctaccgga ctgcgctacg ccccgagcga ggagagtata atccgattta
      361 ggtataaccg tcaagaagaa gataatcagg atgaacagtt ccctcaagct agcgtatctc
      421 tatgctgccc acacccagcg ccatctggag gatctccctt tctggctggg gctggctcac
      481 caggcagacg gaccaatatt agagctcggc tgcggcacag gcaggattct attatctgtt
      541 cttcagtctg gtttacccat catcgggtta gaccgtgacc cggacatgct ggctgtcctt
      601 aaggagaaaa gctgcaaagc ccaaaaatct ccggcgattg tccaggcgga tatgagtgca
      661 ttcaatttcg aacagctttt ctcattgatc atcttaccct gcaatacaat cagtaccttg
      721 agcgattcat cgcgcgtcac catgctaaat gcggtggtag atcatcttgc agagggaggt
      781 atttttgcga ccagcctccc taatccgctt ttattatcaa acctccctga gcatgccgaa
      841 ccagaaatcg aagagagttt tccacatcca ttggatggcg ctcctgtaaa agtgagcagc
      901 tcctgggagc acgatcagca cacattttcc attttgtggg attatgaata tggcctgcct
      961 tcgggtgata tagaacacct gacattgacc agctctcacg gattaatccc catttctcag
     1021 tacctggctg aatttgaaac cgctggattg aatatcctcc atcaatatgg ggatttcaat
     1081 aagcgcctgt tcaaccgccg ggctaacagc ctgattttcg tggcacaaaa accctgatgt
     1141 gcatgattaa cccggatgcg atttatgtag atccggacta tccgatattc ggaatcaaac
     1201 acccacgtgg agttaatctc tcccacgtgg gtggattggt cctggttctt ctatttatcg
     1261 atcagtaacg cagcaaagcg ccaatattct caaaggagcc taccagctgc ggatcgtgga
     1321 ggacttcgac atctccgccc tgcgccaata cctggcggac tgcttcttcc acagcatcag
     1381 ggagctgctt aaaaacgccg ccgcagaaag gacatagatc taatttttcc acagtcacat
     1441 actcacatcc caggcattgg taacccggag ctcgaaaacc atcccgcaag attaaagttt
     1501 gtacccgacc tgaacgaacc gctcctagag tatcttcgag ctggagggta cctccgcgtc
     1561 ctttggaagc gccagtgatc atcgcatcga ccagtttttt ctcccgcttc agctctgccg
     1621 catcacccac cttcattgcg cgctccagga tttccggttc cgtggctgcc atactgactg
     1681 gaaatgtgcc cacaaccagg ctctgccagg ccttggataa gtggctccga aacgcagcca
     1741 cattgtcatc cgttccgccg attaagatcc ggcggacatt gttttctgtg aaaaattgga
     1801 cggcgaaatc aactgcatct ttgatattcc ggtcggttaa ttcatctaca taatcggatt
     1861 ctttggcatt gcctgatcta cggcctgccg cctgagatcc tccacctcgt ttggcatggc
     1921 gaattgtttc acccataacc ccttcctgct cactcagctc gcccaggtga aatttaaata
     1981 aacgcgcgcc ttgtttatca accacaacaa ccccataacc gccaaatgca tcgagaacat
     2041 ctgcgagtgg tttaacatat gggtggtcgc cagttctgac ccggggccgg agacggacgt
     2101 gcagcgggta tgccctgaag aacccttctg cagagcagga gaatatcgcg acacttcgtc
     2161 cagtccaatt atgctcatgg tcaaaatagc gcagtactgc agcaacatca tccggttggt
     2221 tgaccccttt tagcatgctg cgcaaatgca gcctgtgtcc atccgtgctg ctctctgctg
     2281 gatcggtgtt ctgataaacg ctgagaatag gggactttgc ctggtagctc aacaactcct
     2341 gcaggtcacg atcagtcaac atttatagcc tcctgttcat gaaaagtgcc tgatggctgc
     2401 ttgcagacga atgcgtgaag ctcttcagca tcaagggttg ggacgtttat ccagggtcac
     2461 ctccagttta atttcgtcac tgccgcgaaa aatcgtcagc tcaacggtct caccgggtcc
     2521 tttatttttg accaggtagc tgatcaaatc gttgaagttc gttaccggct catcatcgat
     2581 cgcggtaatt aaatcaccac ccgatggtaa gcctgtgata tcggaaggta aattgccaga
     2641 tctgaccccc gcccggtctg ccggcccacc aggggttaca cctgttacgt aaacacctgc
     2701 ggcggtgggc agatcaagcg cttcctgata tacgagggtc agatcatcca gactggtaat
     2761 gccaagatat ggatagtcgt atttcccgtc ggcaatcagg gaaggagcca ccttcttgac
     2821 aatattaatc gagaccgcga atccgatccc tgaattggtt ggctcaccag tggtcgtgaa
     2881 attagaggtc cggatcgatt cgttgacccc gaccacctct ccctgtaaat ttagcaaagg
     2941 tccgcccgag ttaccaggat tgattgctgc atccgtctgg ataatatcgc cagctgagaa
     3001 tgtgccccca tccgaggtcg tatgcagcga ttccaacgtg cgaccagtcc ccgaaacgat
     3061 cccggttgtc atagtgcccg ccagaccaaa ggggttgccg atcgcaatga cagtctgccc
     3121 aacctggacg gcatccgagt cacccagggg tagaggaaag agttcgctct ccggagcatc
     3181 cactttaatg atcgccaggt ctgaatccaa atcctcaccg atgacttcgc cgcgggcttt
     3241 aaatccggaa gcgaaggcta cttccaaatc tgtcacccct tcgatcacat gatagttagt
     3301 caccacatga ccctgcgtgt cgatcacaaa gccagatcct aaaccacccc cctcttccgt
     3361 caatacacgg attgaaacta cacccgggtt aacccgttga tacaagccaa tcaatgtgtc
     3421 ctgggctgaa atcatatcca atacagtaac ttgagcctcc gatgcctcgg ttaccgcagg
     3481 aacgacggcg accggaggat tagttgcatc cgtagagggt gattcaacct ctgtttgact
     3541 tgtgggcagc gcagtaaccg tctggcatgc caggcctgat aacgctaaca ccagaattgc
     3601 caggataatt agacttgtcc gcttcattaa caactcctat ctgcttgtat acaccgccca
     3661 ttgcgacgga taattcactt aatctgcgcc aattcctgaa ataatgaacg ctgtttatct
     3721 gaaagattcc gaggaatcga tactttaatc cgcacataaa gatcgcccag gcggtctggc
     3781 tgcttgagcg tgggcagacc acgtccagcc agtcgaaatg tctggttatt ctgggtgccc
     3841 ggaggaatag tcaggacaac ttttcccgaa aaagtatcaa cctgggtgtc cccgccaagc
     3901 actgctgtat atacatcgat ggaaacatcg gtatagaggt catccccttt ccgctcaaaa
     3961 cggggatccg aagcgacctg aattaccaga tacaaatcac tcttcggtcc ggatgctcca
     4021 ggggaaacca cctggggcac gcgaactttc gtccccgtgc gtgcgccttt aggaatcttg
     4081 acttctaacc ggcggccatc gatttccatt aagcgggttg cgccagaaaa tacttcctga
     4141 aggctaatct caacctgctg ttcgtagctg ggctgcggtc gtggcgaggc gcgtccggtg
     4201 ctgcgcggct gctggcctgg catgcctcca aagatgcgcg tgaaaaattc cgaaaattca
     4261 ccgcctaaaa catcattgat atcgccatat tcaacccgca catttccacc tgggtttgca
     4321 tttgagcggg tgaaccaatc ttcccagtta aaattcccag ccgacccacc atgctgctgg
     4381 taccggaaat acgagtcccc cagctgatca tagcgggcct ttttctcggc atcacttaat
     4441 acctggtaag cctcattcaa ttctttaaat ttatcttcag cggttttatc g
//
LOCUS       DATMXK010000376         2687 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_138126, whole genome shotgun sequence.
ACCESSION   DATMXK010000376 DATMXK010000000
VERSION     DATMXK010000376.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2687)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2687)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2687
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138126"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2282)
                     /locus_tag="VN363_06990"
     CDS             complement(<1..2282)
                     /locus_tag="VN363_06990"
                     /inference="COORDINATES: protein motif:HMM:NF019156.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84293.1"
                     /translation="MLKRFVRIFGGNPNQRTIDKFTEIVDEINSLESATEALSREELT
                     HKTAEFRARLTEGETLDDLLPEAFAVVREASKRTLGLRHYDVQLIGGIALHQTTIAEM
                     RTGEGKTLVATLPVYLNALTGRGVHLITVNDYLARRDARWMAPIYNLLGMSVGVLQMA
                     ARTENGKKAFLVDLGRTSPHEDQHQLLMVPRAEAYQADITYGTNSEFGFDYLRDNMTM
                     RLEDRVQRGHFYAIIDEVDNILIDEARTPLIISGPAQDEGEWYIRMAQVVRSLRPEDY
                     EVSEKDRNVTLTEIGEVHAEELIGMPMRDPDRPEDITPEQARMLGYLEQALRAQFLFR
                     RNKDYLVQAGKVVIIDEFTGRLMPGRRWSDGLHQAVEAKEGVKVQSESVTYATITIQN
                     YFRMYEKLAGMTGTAVTEAEEFDQIYKLEVLAIPTNLEYQAMRPELSLTTIEARDPEG
                     YKYTYYTHTNDPEKQAVYFKRKDYPDVIYRTQETKLRSIAQEILRYHVQGRPMLVGTT
                     SVELSERVSTRLRAEALRRLALTNLIRQAWVSTNKREEDGRVIPELQFLNQPIEDLDI
                     PAMRKMARDLGISFNPEDPENIHRLLGILNIPAAEPERLIADLKSGIPHEVLNARKHT
                     EESQIIAGAGAFGAVTIATNMAGRGVDIKLGGELAEEILSAINRVLSRAGHEDPYDMT
                     MEQRQTALLAMDPAEFGIYGSEVDYFLQHMKDMERVRELGGLHVIGSERHEARRIDNQ
                     LRGRAARQGDPGSSRFYLSM"
BASE COUNT          541 a          718 c          762 g          666 t
ORIGIN      
        1 tccatggaga ggtaaaaacg cgatgatccg ggatcgccct ggcgggcagc gcggccgcgc
       61 agctggttat caattcgccg ggcttcgtgg cgctcggagc cgataacatg caaaccgccc
      121 agctcacgca ctcgctccat gtctttcatg tgctgcaaga aataatcgac ttcgctgcca
      181 tagatgccga attcagccgg gtccatggcc agtaaggcag tctggcgttg ttccatggtc
      241 atatcgtatg gatcttcatg cccggcgcga ctgaggacgc gattgattgc cgaaagaatc
      301 tcctccgcca gctcgccgcc caacttaata tctactccac gaccggccat attggtggcg
      361 atcgtcacag ccccaaatgc acccgctccg gcaatgatct ggctctcttc ggtgtgcttg
      421 cgggcgttaa ggacctcgtg aggaatgccc gatttgaggt cggcgatcag gcgctccggc
      481 tcggccgcgg ggatgtttaa aatgcccagc agccggtgaa tattttccgg gtcttccgga
      541 ttgaaggata tccccaggtc gcgggccatc ttgcgcatgg ccgggatgtc cagatcctca
      601 atcggctggt tcaggaactg cagctccggg atcacgcggc catcttcttc gcgcttattg
      661 gtggagaccc atgcctggcg gatcaagttt gtcaaagcca gccggcgcag ggcttcagcc
      721 cgcagacggg tggagacgcg ctcagaaagc tcgacggaag ttgtgccaac cagcatcggg
      781 cgaccctgca cgtggtagcg caggatttcc tgggcgatcg agcgcagctt ggtctcctgg
      841 gtacggtaga tgacatctgg atagtctttg cgcttaaaat agactgcctg tttttcagga
      901 tcgttggtgt gcgtgtaata ggtatattta tagccttcag gatctcttgc ttcaatggtc
      961 gtcaggctta attccggacg catggcctgg tattccaggt tggtggggat tgccaggact
     1021 tccagcttgt agatctgatc gaattcctcc gcctcggtga cggcggtgcc ggtcatcccg
     1081 gccagctttt catacatgcg gaagtaattc tgaatggtga tcgtggcgta ggtgaccgat
     1141 tcgctctgga ccttgacgcc ttctttggct tctactgcct gatgcaagcc atccgaccag
     1201 cgccgtccag gcattaaacg cccggtgaat tcatcgatga tgactacctt cccggcctgg
     1261 acaaggtaat ctttattccg tctgaacaaa aattgagccc gtaaagcctg ctccagatag
     1321 cccagcatgc gcgcctgttc gggggttata tcctccgggc gatcgggatc gcgcattggc
     1381 ataccaatca attcttcagc atgtacttcg ccaatctcgg tcagggttac gttgcggtct
     1441 ttttcgctga cttcgtaatc ctcggggcgt aaactgcgca ctacctgcgc catgcgaata
     1501 taccactcgc cttcatcctg agccggcccg gagatgatca acggcgtgcg ggcttcatcg
     1561 atcaggatat tatccacctc atcaatgatg gcgtaaaaat gcccccgctg gacacggtcc
     1621 tccaggcgca tggtcatatt gtcccgcagg taatcgaagc caaattcgct gttggtgccg
     1681 taggttatat ctgcctggta ggcttcggcg cgtggaacca tgagcagctg gtgctgatct
     1741 tcgtggggtg aggtgcgccc caggtcgacc aggaaggctt ttttcccgtt ctcggtgcgg
     1801 gctgccatct ggaggacgcc aacactcatg cctaatagat tgtagatcgg tgccatccag
     1861 cgtgcgtcgc ggcgggccag ataatcattg accgtaatga gatggacgcc gcgcccggta
     1921 agggcattca gatagaccgg taaggtggct accagggtct taccttcccc ggtgcgcatt
     1981 tcggcgatcg tcgtttgatg cagggcgata ccaccgatca gctggacatc ataatggcgc
     2041 agccccaggg tgcgtttgct ggcctcgcgc acgacggcaa atgcttcggg taatagatcg
     2101 tccagagttt cgccttctgt caggcgtgcc cggaattcag cggtcttgtg ggtcaattct
     2161 tcgcggctca gtgcctcggt tgcgctctcc aggctgttga tctcgtctac gatctcagtg
     2221 aacttatcaa tggtgcgctg gttagggttg ccaccaaaaa tgcgtacaaa tctttttagc
     2281 ataaattgca cctttcgggt gcgattatag catagtgagt ttaaaatcag ctaagaggct
     2341 caggcctgtg cggaccggag gatggtaaaa aatggagagg gaaactttaa tcttaaagaa
     2401 ttcttttgtt tcggagttga ccgagactgg catgctataa tccattcagc taaattcaca
     2461 acctggatgg ccaattttcg ggatggtgtt tgggggctct gacacatggc agtgggttgg
     2521 cggtttttga tccgccagat ttaatcataa ttgttcaagg ctggtagtgc atgtcttttg
     2581 tccatttaca cgtccattct gaatactccc tgctggatgg gtttagcgac ctcgacaaac
     2641 tggtaactcg cgccagggaa atggagatgc ccgccctggc gctgacc
//
LOCUS       DATMXK010000377         6055 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_63209, whole genome shotgun sequence.
ACCESSION   DATMXK010000377 DATMXK010000000
VERSION     DATMXK010000377.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6055)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6055)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6055
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63209"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..444
                     /locus_tag="VN363_06995"
     CDS             <1..444
                     /locus_tag="VN363_06995"
                     /inference="COORDINATES: protein motif:HMM:NF024898.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84294.1"
                     /translation="RFGHQFADIIEGDGDTIGILISYPGRMLGRLSLTTGRQLWGIHG
                     AIGFIRFARRALPLAFVKEADADEFFINALAVSPAFQGRGIGSQLLSYAEPKARSQHY
                     NKCSLSVARSNLRACHLYERGYRVVETIPCDLPGYAGYHPMVKVL"
     gene            482..1144
                     /locus_tag="VN363_07000"
     CDS             482..1144
                     /locus_tag="VN363_07000"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84295.1"
                     /translation="MLSLAVLVDGIAGAIAIFFSVLLSPLIRPWRLRWGATDAEVAQT
                     LPGDEIVSQPRIGWTHAVTVQAPPERVWPWIVQIGCRRAGWYSYDLLDNGSQPSAEHI
                     LPEHQALAVGDRISMVSRGDFSIPVAAIEPGRALVLGGELPASPTEPIAVTWAFVIQP
                     HGENASRLISRWRASWRPGLFADLAWGVLIEAIGFVMDRKMLLGIKRRAESGNGAVAD
                     NE"
     gene            1385..1855
                     /locus_tag="VN363_07005"
     CDS             1385..1855
                     /locus_tag="VN363_07005"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF014566.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004222 - metalloendopeptidase activity
                     [Evidence IEA];
                     GO_process: GO:0071586 - CAAX-box protein processing
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CPBP family intramembrane glutamic
                     endopeptidase"
                     /protein_id="HWQ84296.1"
                     /translation="MENPQGFLARPLTLLPFALTTLLVGPLPEEPGWRGYALDKLQEH
                     WSALRSSLVLAFFWWAWRLPMFFTRGTGIGELWPIGSLPFWVNFTLSLPATTLLMTWA
                     FNNTRSSTLGAILFHFTANFSGEFLDMPNQFHTSALPGASWPRRRSCGCMVPRE"
     gene            1887..2780
                     /locus_tag="VN363_07010"
     CDS             1887..2780
                     /locus_tag="VN363_07010"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ84297.1"
                     /translation="MKAYPEQHIRLADGRHLSYDNVGAPDGTPVLYFHGVPSARVEWQ
                     MWGDETMLQQLGVRLIAIDRPSVGSSTFQQYRRLSDWPADVAALADRLGLQQFGVLGY
                     SGGGPYAAVCARQIPERLIHTALVSSLAPFDLPGMLEGVAPGNVQFLKLAVQKPWLFR
                     LLYGQIGLLTRLAPGQFLKRALGTFEAADRAAFARPEVHGPILAAHGSGRSQQMDTAL
                     IISAWDFDLGEIAVPVQLWRGEQDHNASAAMFDYLARTIPKTQPHRVPGEGHISLIVN
                     HVPDILKTFTRKLERKTKPCC"
     gene            2792..4402
                     /locus_tag="VN363_07015"
     CDS             2792..4402
                     /locus_tag="VN363_07015"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ84298.1"
                     /translation="MIFVLVLAATAVGIFYHTPGASIEFTTVRGERAVFQGSGLYRYD
                     PAPLADEGVVWDAINLLIGLPLFAVAIYLSQGNSMRGRLLLGGLLFYWFYAYLQLMVT
                     YSFNFLFLDYVTIYALSAVSFFMNLHDIEVARLPAQVSERFPRRLFIGFTFVVSIMLV
                     FLWLGRILPVMASGRFPPELAGLNTLITRGVRFGHGRPLNAGDRDFALAPLPVGLPAG
                     EPQYQLWFAHEHHPAGVDCRALDPGRKDQPHRSRSLSRDLPGRASFCGPVFQERAGGK
                     SAPTVILIHGGGDNSLIWSLVYPGVARAYRVCSYDRPGYGWSETVPTPRSAAQNANEL
                     HLLLSQAGIEPPYLLVAHSIGGLTAHQFTHRYSDEVAGLILLDSMLAEQMVAQGTFWM
                     RLPLVDTAICRLFSSSGLYRFLFDTGRLAPDEPVLKLPVELQPDALALRLRVGLCQAT
                     YAEQAAAIQDVHTLHEKGGLGDVPVAVLNAYETEADRKSLVERQKYVQRLSTQITYTA
                     VGPSGHHIHLDQPDEVVQAILLMGESIR"
     gene            complement(4469..4894)
                     /locus_tag="VN363_07020"
     CDS             complement(4469..4894)
                     /locus_tag="VN363_07020"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ84299.1"
                     /translation="MNGSCILVARQGDLEAFNRLVLEHQDLVYNLAYRMLGNPWDASD
                     ATQEAFLLAYRNLSAYRGGSFRAWILRIATNLCYVELRRRQPHRAKQDWQTDEEIDHV
                     IGHERTKQLLHRVDDGAVQQANDGCSCHEDSDDERENVC"
     gene            complement(4922..>6055)
                     /locus_tag="VN363_07025"
     CDS             complement(4922..>6055)
                     /locus_tag="VN363_07025"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84300.1"
                     /translation="MGGDVWVNHLDTMQTVRLTQDGYNSHPQWALDGSHIAYRKKDQL
                     WVMDLTTKQTFPVSELPVEWFTWSSSSTRLIYFSLDEGLVIWDVTNNTTSTVIPISNE
                     YSIENFVWDSQGETLVFNKGSVVGGSYVVSLEKVNPEKKGTVTIFTTSDMHEVPLLGE
                     VSPGGQWIAFWRWNTGITFFEKEGLPLCTISVPGEQAKCTASKTIPLSEFLDWSLDDQ
                     LAYFTIEQNLVIADPKGSSEQSLVDLAYQSPIYPAWTPDGERIAYSATSIGAGKSSDA
                     QNQSDACIQRRIWVVEVDSKRLHQLTNDDRFCDELPFWSVDGKHILFARLDKENASLW
                     LMHSNGKGLSQIVPELTPKPEPLGKYGYIDWPAWYDWWRPGVP"
BASE COUNT         1163 a         1776 c         1722 g         1394 t
ORIGIN      
        1 cgattcggcc accagtttgc cgatatcatt gaaggcgatg gcgataccat cgggatcttg
       61 atcagctatc ctggccggat gctgggacgc ctgagtctga caacgggcag gcaattgtgg
      121 ggcatccatg gcgcgattgg atttatccgc ttcgcgcgca gggcgcttcc gctggccttc
      181 gtaaaagagg ccgatgcgga tgaattcttt atcaatgctc tggctgtgtc gcccgctttt
      241 caggggcggg gcatcgggtc tcagctgctg tcgtatgcag aacccaaggc caggagccaa
      301 cactataaca agtgctctct cagcgttgcc aggagcaatc tacgcgcctg tcatctgtat
      361 gagcgtgggt atcgagtggt ggaaaccatc ccatgcgatc taccgggata cgcaggctat
      421 caccccatgg taaaggtgct ctaaatttgc agatcatttc tcgctttctg gaggttgttt
      481 tatgctatct ctggccgttc ttgtcgatgg cattgccggc gccattgcta ttttcttttc
      541 ggtcttgctc agcccactga tccggccgtg gaggctgcgt tggggtgcaa ccgacgcgga
      601 ggtcgctcaa accctgcccg gggacgaaat tgtttcacaa ccgcgaattg gctggaccca
      661 cgcggtgact gtgcaggctc caccggagcg ggtctggccc tggatcgtcc agattggctg
      721 ccggcgcgcc ggctggtaca gttacgacct gctcgataac ggctcgcagc ccagcgcgga
      781 gcacatcctg cccgagcacc aagcgctggc cgtgggggat cgcatctcga tggtgtcgag
      841 aggcgatttc tcgattcccg tggcggccat cgagcctggc cgtgccctgg tgctcggcgg
      901 agagctgcct gcgtccccca cagaacccat cgccgtgact tgggctttcg tgatccagcc
      961 gcatggggag aacgcctccc gcctgatctc ccggtggcgt gccagctggc gtccagggct
     1021 gttcgccgac ctcgcctggg gcgtgctgat cgaagccatc ggtttcgtca tggaccgcaa
     1081 gatgctgctg ggcatcaagc gccgggcaga gtcgggaaac ggcgcggttg ctgataatga
     1141 ataggggtaa tgatgagaag ttctgatacc ttcaaaacca cctggctcta ttttgtcctg
     1201 gcgctgggct ggtcgtggct gttctggatc ccgctggccc tgtcggggca ggaagctgcg
     1261 gccttccctt gggtgctgct gttcgccctg ggcggcctgg tgggcggtca tcctgctgct
     1321 cttccctgcg ctcaacctgc tcggcctggt gagcgggcgg ctcttcctgg gagcgtggcc
     1381 gccgttggag aatccccagg ggttcctcgc ccggccgctg accctgctgc ccttcgccct
     1441 gaccaccctg ctggtcggcc ctctgcccga ggagccaggc tggcgcggct acgccctgga
     1501 caaactgcag gaacactgga gcgccctgcg ctcaagcctg gtgctggcct tcttctggtg
     1561 ggcctggcgc ctgcccatgt tcttcaccag gggcaccggg atcggtgagc tatggcccat
     1621 cggcagcttg cctttttggg tgaatttcac gctgagcctg cccgccacca ccctcttgat
     1681 gacctgggct ttcaacaata cccgcagtag tacgttggga gcgatcctgt tccacttcac
     1741 ggccaatttc agcggcgaat tcctggatat gcctaaccag ttccacactt ccgctctgcc
     1801 tggagcgtcc tggccgcggc ggcggtcgtg tggctgtatg gttcccagag aatgagtgat
     1861 cgaaaatcac cccagggatc gagcacatga aagcttaccc ggaacaacat atccgtttgg
     1921 ccgatggccg ccacctcagc tacgacaacg ttggcgcgcc ggacggtaca ccggtgcttt
     1981 acttccatgg cgtgcccagc gcccgcgtag agtggcagat gtggggggat gagacgatgc
     2041 tccagcagct cggtgtccgc ctgatcgcca ttgaccggcc gagcgtgggt tcttccacct
     2101 tccaacagta tcgccgcctg agcgattggc ccgccgatgt ggccgccctg gctgataggt
     2161 tgggtttgca gcagttcggc gtgctgggtt actccggcgg cgggccgtat gccgcggtgt
     2221 gcgcccgcca gatcccggag cgcctgatcc acactgccct ggtgagcagc ctggcgccct
     2281 ttgatctgcc cggcatgctg gaaggggtcg cgccgggcaa cgtgcagttc cttaagctcg
     2341 ctgtccagaa accctggtta ttccgcctgc tctatgggca gatcggcctg ctgacccgcc
     2401 ttgcgccagg acaattcctg aagcgcgccc tggggacgtt tgaagcggcc gatcgggctg
     2461 cctttgcccg cccggaagtc catggcccga tcctggcggc ccacggcagt ggccgcagcc
     2521 agcaaatgga tacggcgctc atcatcagcg cgtgggattt cgacctcggc gagatcgccg
     2581 tgcccgtgca gctctggcgg ggcgagcagg accacaacgc ctctgcggcc atgttcgatt
     2641 acctggctcg aactatcccc aaaacccaac cccaccgggt ccccggtgag gggcacatct
     2701 ccctgatcgt caaccacgta ccggacatct tgaaaacttt caccaggaaa cttgaacgaa
     2761 aaaccaaacc ctgctgctga tcctgtgcct cttgatcttc gtactggtgc tggcggccac
     2821 agccgtcggt attttttacc acactccagg tgcttcaatc gagttcacga ctgtacgcgg
     2881 ggagcgcgcc gtctttcaag ggagcggcct gtatcgctac gatcccgccc cactcgccga
     2941 cgaaggggtg gtctgggacg cgatcaacct gttgatcggc ctgcccctgt tcgccgttgc
     3001 tatctacctg agccagggga actctatgcg agggcgattg ctgctgggcg gcctgctgtt
     3061 ttactggttc tatgcctatt tgcagcttat ggtcacttat tccttcaatt tcctgttcct
     3121 cgattacgtg acgatatacg ctctgagcgc ggtgtctttt ttcatgaacc tgcacgatat
     3181 cgaggtggcg cgcctgcctg ctcaagtttc ggagcggttc ccgcgccgct tgttcatcgg
     3241 ctttaccttc gtcgtaagca tcatgctggt gttcctttgg ttggggcgca tcctcccggt
     3301 catggccagc ggccgctttc cgcctgaact ggcgggcctg aacaccctga tcacccgggg
     3361 ggttcgattt gggcatggtc gtcccctaaa tgctggcgac cgggattttg ctctggcgcc
     3421 gctccccgtg ggactacctg ctggcgagcc tcagtatcag ctatggtttg ctcatgagca
     3481 tcatcctgcc ggcgtggatt gccgcgccct tgatccagga aggaaagatc aacctcatcg
     3541 aagccgttcc ctttctaggg atctgcctgg tcgggctagt ttttgcgggc ctgtttttca
     3601 ggaacgtgca ggaggaaaga gcgccccaac ggtgatcttg atccacggag gtggagataa
     3661 ttcgcttatc tggagcctgg tatacccagg ggtggccagg gcctatcggg tgtgcagcta
     3721 cgaccggccg ggctacggat ggagcgaaac ggtgcccacg ccgcgctcag ccgctcaaaa
     3781 tgccaatgag cttcaccttt tgttgtctca agccgggatc gagccaccgt atctcctggt
     3841 cgcccattcc attggcggcc tgacggccca ccagtttact caccgctatt ccgatgaagt
     3901 tgcgggactg atccttctgg acagcatgct ggcagaacag atggtcgctc aagggacgtt
     3961 ctggatgagg ctccctttgg tggacacggc gatctgccgc ctgttttcct ccagcggttt
     4021 gtaccgtttt ctattcgata ccggcaggct ggcgcccgac gagccggtct tgaaactacc
     4081 ggtcgagctg cagccagacg cgctggcgct gcgtttacgc gtggggttgt gccaggccac
     4141 ctacgcggaa caagccgctg ccattcagga tgtccacacc cttcacgaaa agggcgggtt
     4201 gggagatgtg ccggtagccg tcctgaatgc ctacgagacc gaagcggatc gaaaaagcct
     4261 ggtagaacga caaaaatacg tacagcgact atccacacaa attacctaca cagccgttgg
     4321 gccgagtggg caccacattc atctcgacca acccgatgag gtcgtacagg ccattttact
     4381 gatgggagag tcaattcgat aggagtttaa agaaatgaac acaaatcgag agcataacgc
     4441 cagtctgccg atcaggcgta acctcgggct aacatacgtt ctctctttca tcatcgctat
     4501 cctcatggca gctgcatccg tcgttggcct gctgtaccgc accgtcatct acccgatgga
     4561 ggagttgctt cgtacgttcg tgtccaatga cgtggtcaat ctcttcatcg gtctgccaat
     4621 cctgcttggc tcgatgtggc tggcgacggc gcagctcaac gtaacagagg ttggtcgcga
     4681 tgcgtaaaat ccaggcgcgg aacgagcctc cccggtaagc ggacaggttc cggtaagcca
     4741 ggagaaaagc ctcctgggtt gcatcgctcg catcccaggg gttgcccagc atccggtaag
     4801 ccaggttata gaccaaatcc tggtgctcca gaaccaggcg gttgaacgcc tccaggtctc
     4861 cttgtcgagc aacgagaata caagatccat tcatagcagg ccggtcagaa atatcagatt
     4921 ttcaagggac tcccggccgc caccagtcat accaggctgg ccagtcaata tatccgtatt
     4981 tgccaagggg ttctggtttt ggggtcaatt caggaacgat ttggctcagt cccttgccat
     5041 tagaatgcat taaccacagg ctcgcattct ctttgtccag ccgagcgaag agaatgtgct
     5101 ttccatccac agaccagaag ggcaactcat cacagaaacg atcatcattg gttaactggt
     5161 gaagtcgttt agaatcaacc tcgactaccc atattcgccg ctggatacag gcatcactct
     5221 ggttttgtgc atcagaggat tttcccgcac caatagaagt ggcactataa gcgatgcgct
     5281 caccatccgg agtccaggct ggatagattg gggactggta agcgagatca accagggatt
     5341 gctctgaaga cccttttgga tctgcaataa ccagattctg ttcaatcgtg aaataggcta
     5401 gttggtcgtc taaagaccag tccaagaatt cactcaaagg gattgttttt gaggctgtgc
     5461 atttggcctg ttcaccagga acggatatcg tgcacaatgg taatccttct ttttcgaaaa
     5521 aagtaattcc tgtgttccaa cgccaaaatg caatccattg ccctcctggt gatacctctc
     5581 caagaagtgg aacctcatgc atgtcactgg tcgtaaatat ggtgacagta cctttcttct
     5641 ccgggtttac cttctccaga gataccacgt atgacccgcc cacgacactc cccttgttaa
     5701 ataccaatgt ctcaccctgg ctatcccaaa cgaagttttc aattgagtat tcgttggaga
     5761 tagggatgac cgtgctggta gtattattgg ttacatccca aataacaagg ccctcgtcga
     5821 gagagaagta gatcaggcga gtgcttgatg aagaccaggt aaaccattcg accggtaatt
     5881 cgctcaccgg gaaagtttgc ttcgtggtca aatccatcac ccaaagctga tcttttttgc
     5941 gataagcaat gtggctgcca tcaagtgccc attgagggtg actgttgtat ccatcctgcg
     6001 tcaatcggac tgtctgcata gtatccaggt gattgaccca cacatcacca cccat
//
LOCUS       DATMXK010000378         3879 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_138694, whole genome shotgun sequence.
ACCESSION   DATMXK010000378 DATMXK010000000
VERSION     DATMXK010000378.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3879)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3879)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3879
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_138694"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..823
                     /locus_tag="VN363_07030"
     CDS             <1..823
                     /locus_tag="VN363_07030"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84301.1"
                     /translation="ILTACLLALMPAAVILAQTGIVIDGAFEDWAGMPGIVDPGGEDD
                     ETELSDADITEFRAYGDLSAFYMLNAWDDTAFNPSGTGAGATIRAADGTYYRIYTTVQ
                     GNPATVPLSSLMIYSCTDASCADQTAVCTGAACTGAQAGSDVDWVDPFTGRPTPDCNG
                     TACGTQDAAVEFSIPWTLIGGMPADGQTIFVQFGSYPTAPSPAPKDNTGANGVTCRNT
                     GGVIECYVSDPTAITLSSLQATAASDTPGLLAGGLVLAVLLVAMLYEKKLNLQVG"
     gene            complement(940..1545)
                     /locus_tag="VN363_07035"
     CDS             complement(940..1545)
                     /locus_tag="VN363_07035"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWQ84302.1"
                     /translation="MRKLLTTTFITLDGVMQAPGGPGEDDDGGFTQGGWSVHYWDEAL
                     DAAMGEIMGREFEFLLGRKTYEIFAAYWPTSTEVGADVINNARKHVVSRSLEAVDWNN
                     TTLIRGDAVPEIARLKAMDGPELQVHGSSNLIQTLLKNDLIDEIHLFIFPVAIGKGKR
                     YFGDGTDPAAFKLASSTTSNSGVIIATYVRDGALQTGTFGV"
     gene            complement(1557..2921)
                     /locus_tag="VN363_07040"
     CDS             complement(1557..2921)
                     /locus_tag="VN363_07040"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84303.1"
                     /translation="MNEKTFGPESEPSRPSAPYPGKQPAASQSGAPASTTEVHAGTPA
                     SGKPAAGTPASGKPASGTPAAGTPWNLTTLALLLLPLIILGGVIWLFLTTGGGLDLRA
                     PAPVEALTFERSVLQPGLIELHVRNASPEPLTIAQVIVNNAVWPFETRSGSSTLPRLS
                     TDVIHLAYPWSYGEAYAIRLFTANAIAFDTEIPVAFSTPQPDSQTFLSFALIGLYVGV
                     IPVYLGLFWFPALRQLGKRWMVFLLALTAGLLVFLGLDTLSEALGQAAAVPGAFQGVG
                     LVGIGALSTFFLLEAISQRQGAGGRSEADQRLSLAFMIAVGIGLHNLGEGLAIGAAFN
                     VGEIALGAFLVVGFILQNITEGLGIIAPVLRDRPGIGRLALMGAIGGAPAILGSWIGG
                     FSPSPTLAVLFLGIGTGAVFQVVFAIARLIQKDTTRTPMPLTVFSGIVTGMLLLWVTG
                     LMIK"
     gene            complement(2933..>3879)
                     /locus_tag="VN363_07045"
     CDS             complement(2933..>3879)
                     /locus_tag="VN363_07045"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWQ84304.1"
                     /translation="HAGHAAAPAAPAAQMDHTAAAHAMPMAVGEVDHARNGFDPTAIL
                     TDFDYGVVSTLPSGQTLREYDIFVINQEVEVAPGIFYPAWTYNGRIPGPTLRCTEGDH
                     VRILFRNGTAHPHSLHFHGIHAAEMDGVPGTPGMLEPGQSFTYEFDAEPFGLHLYHCH
                     AAPLARHIAKGLYGAFIIDPKEGRPAVDHEYVMVMSGFDVDFDDENDFYAVNAIPFHY
                     QLHPIQFRVGETVRIYLVNILEFDLINSFHLHANFFNYYPTGTSLTPSEFTDIIMQTQ
                     AQRGILEFSYKYPGLYMFHAHNTEFAELGWTGNFEVLP"
BASE COUNT          714 a         1164 c         1313 g          688 t
ORIGIN      
        1 gattcttact gcgtgcctgc tggcgctcat gccggcggcc gtgatcctgg cccagaccgg
       61 gatcgtgatc gacggcgcct tcgaggattg ggccgggatg cccggaattg ttgacccggg
      121 tggtgaggat gatgagaccg aactctcgga tgccgatatc acggaatttc gcgcctacgg
      181 cgatctttcg gcattctaca tgctgaatgc ctgggacgat acggccttca atccttcggg
      241 caccggggcc ggcgcaacca tccgcgctgc ggatgggact tactaccgga tttacacgac
      301 tgtacagggc aatccggcca cagtaccgct ttccagcctg atgatttaca gctgtaccga
      361 tgccagctgc gcagaccaaa ccgcggtgtg caccggggca gcctgcaccg gcgcccaggc
      421 cggctcggac gtcgattggg tggatccatt taccgggcgg ccaaccccgg actgcaacgg
      481 caccgcctgc ggcacccagg atgccgcggt ggaattttca atcccgtgga cgctgattgg
      541 cgggatgccc gcggatggcc agacgatctt cgtccagttc ggctcctacc ccacagcccc
      601 gtcgcctgca ccaaaggata atactggcgc caacggcgtc acctgccgca atactggcgg
      661 cgtgatcgag tgctatgtct ccgaccccac cgcgataacc ctgagcagcc tgcaggccac
      721 ggccgcgtcg gatacgccgg gcctgctggc aggcggcctg gtcctggcag tcctgctggt
      781 ggcgatgctc tatgagaaga agctgaattt gcaggtcggg taaaaaattt cagcctgacg
      841 gtttgattta taaaccagac cccaggggtt taaaaatccg aaggatttac ccctggggtc
      901 tgctgtttgg atggtccagt tcaggataca gggatcgatt tatactccaa acgtcccggt
      961 ctgcagggcg ccatcgcgca cataggtggc gatgatcacg cctgaattgg aggttgtgct
     1021 gctggcgagc ttgaaagcag ccgggtcggt gccgtccccg aagtagcgct tgcctttgcc
     1081 gatagcgacc gggaagatga acaggtggat ctcatcgatc agatcattct tcagcagggt
     1141 ctggatgaga ttgctgctgc cgtggacctg cagctcgggc ccgtccattg ctttcagccg
     1201 ggcgatctcc gggaccgcat cccctctgat cagggtggta ttgttccaat cgaccgcctc
     1261 caggctgcgc gagacgacat gcttgcgggc gttgttgatc acatccgccc cgacctcggt
     1321 ggaggtgggc cagtatgcgg cgaagatttc ataggtcttg cggcccagca ggaactcgaa
     1381 ttccctgccc atgatctcgc ccatcgccgc gtccagcgct tcatcccagt aatggaccga
     1441 ccagccgccc tgggtaaagc cgccgtcatc atcctcgcca ggtccaccgg gagcctgcat
     1501 caccccgtcc agggtgataa atgtggtggt aagtagtttg cgcatggtta acctccttat
     1561 ttgatcatca gcccggtcac ccacaaaagc agcatccccg tcacgatccc cgagaagacg
     1621 gtgagcggca tgggggtgcg ggtggtgtct ttctggatca ggcgggcgat agcaaaaacg
     1681 acctggaaga ctgcgccggt gccgatgccc aggaagagca ctgccagggt gggcgagggg
     1741 gagaatccgc caatccagct gcccaggatg gccggcgcgc cgccgattgc gcccatcaat
     1801 gccagccggc cgataccggg cctatcccgg agcaccgggg cgatgatccc cagcccctcg
     1861 gtgatattct gcaggatgaa ccccactacc aggaaggctc ccagggcaat ctcgccgaca
     1921 ttgaaggccg ccccaatcgc caggccttcc cccagattgt gcaggccgat gcccaccgcg
     1981 atcataaaag ccagcgagag gcgctggtcg gcttcactgc gcccgccggc cccctggcgc
     2041 tgggagatgg cctccagcag gaagaaggtg ctcagggcgc cgatgcccac cagccccacc
     2101 ccctggaaag cgcccggcac cgccgcggcc tgccccaggg cttcggagag ggtatccagg
     2161 ccgaggaaga ccagcagccc ggctgtgagc gccagcagga agaccatcca gcgcttgcca
     2221 agctggcgca gcgccgggaa ccagaacagg cccaggtaca ccgggatcac ccccacgtac
     2281 aggccaatca gggcaaagct gaggaaggtc tggctgtccg gttggggggt gctgaaggcg
     2341 accgggatct cggtatcgaa ggcgatggca ttggcggtga acaggcggat ggcataagcc
     2401 tcgccgtacg accaggggta cgccaggtgg attacatccg tgctcaggcg gggcagggtg
     2461 ctggagccgg agcgggtctc gaaaggccag acggcgttgt tgacgatcac ctgggcgatg
     2521 gtgagcggct cggggctggc attgcgcaca tgcagctcga tcaggccggg ctgcaggaca
     2581 ctgcgctcaa aggtcagcgc ctcgaccggg gccggggcgc gcaggtccag gccgccgccg
     2641 gtggtgagga acagccagat cacgccgccc aggatgatca gcggcaggag cagcagcgcc
     2701 agggtggtca gattccaggg cgtgccggcg gccggcgtgc cggatgccgg cttgccggat
     2761 gccggcgtgc cggcggctgg cttgccggat gccggcgtgc cagcgtgcac ctcagtggta
     2821 ctggcaggcg cgccggactg ggaagcggcc ggctgtttcc cgggatacgg ggcagacggc
     2881 cgggagggct cactttcggg gccaaacgtt ttttcattca ccgccggcct ccttatggaa
     2941 gaacttcaaa gttaccggtc cagcccagct cggcgaactc ggtattgtgg gcgtggaaca
     3001 tgtacagccc ggggtatttg tagctgaatt ccaggatgcc gcgctgcgcc tgggtctgca
     3061 tgatgatatc ggtgaactca ctcggcgtga ggctggtgcc ggtggggtaa tagttgaaga
     3121 aattggcatg caggtggaag ctgttgatca ggtcgaactc caggatattg accaggtaga
     3181 tgcgcaccgt ctcgcccacc cggaactgga tggggtggag ctggtagtgg aaggggatgg
     3241 cattgacggc gtagaaatcg ttctcgtcgt cgaaatcgac atcgaagccg gacatgacca
     3301 tcacatactc gtggtccacc gccgggcggc cttctttggg gtcgatgatg aaggctccgt
     3361 acagcccctt ggcgatatgc cgggcgagcg gcgcggcgtg gcagtggtac aggtgcagcc
     3421 cgaacggctc ggcgtcgaac tcgtaggtga agctctgccc cggctcgagc atgccgggcg
     3481 tgccggggac gccgtccatc tcggcggcgt gaatgccgtg gaagtgcagc gaatgggggt
     3541 gagctgtgcc gttgcggaag aggatgcgca cgtggtcacc ctccgtgcag cgcagggtcg
     3601 gccccgggat gcgcccgttg taggtccagg cggggtagaa gatgcccggg gcaacctcca
     3661 cctcctggtt gataacgaaa atgtcgtact cgcgcagggt ctgcccggat ggcagggtgg
     3721 agacgacccc gtaatcgaaa tcggtgagga tcgcggtcgg gtcgaagccg ttgcgggcgt
     3781 ggtccacctc gccgacagcc atgggcatgg catgggcggc cgcggtgtgg tccatctgag
     3841 cggccggcgc ggcgggtgcg gctgcgtgcc cggcatggg
//
LOCUS       DATMXK010000379         2514 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_17068, whole genome shotgun sequence.
ACCESSION   DATMXK010000379 DATMXK010000000
VERSION     DATMXK010000379.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2514)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2514)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2514
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17068"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..888
                     /locus_tag="VN363_07050"
     CDS             <1..888
                     /locus_tag="VN363_07050"
                     /inference="COORDINATES: protein motif:HMM:NF014674.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HWQ84305.1"
                     /translation="SGDLQKGVLRIEPNVSVRLAGSPALGTRTEIKNLNSFRALERSV
                     AYEIARQSECLRQGQRVIQQTLGWDDLREVTVPQRSKEDADDYRYFPEPDLPPLVIDP
                     LTIEQVRAELPELPLAKYQRFLTQYALNDYDAGLLVAEQEVAAYYEQAVKSTSGINPK
                     AVANWITGELFSLLNLAGLRIASAPAPAELAALVAMVGRGELNQNAAKTVLAEMFQSR
                     EPAASVSARLGLSQISDPQALAGLVREVLANYPEQVQEFRLGKAGLGRWLFGQVMRAA
                     QGRANPQVLQAELDCQLQA"
     gene            1169..2455
                     /locus_tag="VN363_07055"
     CDS             1169..2455
                     /locus_tag="VN363_07055"
                     /EC_number="1.4.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559056.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Glu/Leu/Phe/Val dehydrogenase"
                     /protein_id="HWQ84306.1"
                     /translation="MAELINAFKMAQSQFDHVATQLNLDPQVSEILRWPAREFKFMIP
                     VRMDDSAIKVFFGMRVQHSDARGPSKGGIRFHPSETLDTVRALAMWMTWKCAVADIPL
                     GGGKGGVAVDPATLSISEQERLCRGWIDQIWRNIGPRQDVPAPDVGTNPRMMGWMMDE
                     YSKLVGQYTPGVITGKPVGGGGSLGRTEATGFGVIYNVREAMKHLKIDSTKSVAAIQG
                     FGNVSQYAAIGFSELLGGKVACVSYWDRQDRTSYTVLHPEGINPRFLQTITNTYGAID
                     KDAAVKAGYRIASGDAWIEQDVDVLIPAALEGQINAETVKHVSDRVKLVAEGANGPTT
                     PEADAVFKQNNVFVIPDFLCNAGGVTVSYFESVQNDMNFYWTSEEVLQRLDTKITQAF
                     DSVVKMAESEKVFMRDAAYMVAIDKVVKAMELRGLI"
BASE COUNT          542 a          713 c          710 g          549 t
ORIGIN      
        1 tccggcgacc tgcagaaagg cgtcctgcgc atcgagccga atgtttcggt gcgactcgcc
       61 ggcagcccgg ctttgggcac ccggaccgag atcaaaaatc tcaatagttt ccgtgctctg
      121 gagcgctccg tcgcctacga gatcgctcgc cagagtgagt gcctgcgcca ggggcagcgc
      181 gtgatccagc aaacgttggg ctgggatgac cttcgtgaag tgacggtccc ccagcgcagt
      241 aaagaagacg ccgatgatta tcgctacttt cccgaaccag acctgccgcc tctggtaatc
      301 gatcctctta cgattgagca ggtccgcgct gagcttcctg agctgcctct cgccaaatac
      361 cagcgtttcc tcacccagta tgctttgaat gattacgatg ccggcctgtt ggtagccgag
      421 caagaagtcg ccgcatatta cgaacaggcg gtaaagtcta cctccggaat taatcccaaa
      481 gcggttgcca actggatcac gggagagctg ttcagcctgc tcaacctggc tggactgcgg
      541 attgcttcag cccccgcgcc ggcagagctg gcagctttgg tcgccatggt cgggcggggt
      601 gagctgaacc agaacgccgc caaaaccgtg ctggctgaaa tgttccaaag cagagagccc
      661 gctgccagcg tcagcgctcg cctcgggctg agtcagatat ctgaccctca ggcgctggcc
      721 gggttggtcc gggaggtcct ggctaactat ccagagcagg tccaggagtt tcgcctcggc
      781 aaagctggcc tgggtcgctg gctctttggg caggtcatgc gcgccgccca gggccgggcg
      841 aacccccagg tcctccaggc cgagttggac tgccagctgc aagcctgaag cccggtgtgg
      901 catgattccc cacatgttaa acacgcctgg ctgtccttta atgaggaagc acgtttctta
      961 tcgatatgcc tttcgtcact tgacgttcaa gcccgctctg gttaacctat aacaggctgt
     1021 tgagcgatgg actcgatctg agagacaatt gttcatgcga aatcgaggca aaatacacct
     1081 ggtaacaagc gggcgccaaa ccggggtgcc cgtttatatt ttaagcaaac ctattcgctt
     1141 gcaaaacgaa atggaggtct gaacccgaat ggctgaattg atcaacgcgt ttaagatggc
     1201 tcaaagccag tttgaccatg tcgctaccca attgaacctg gacccgcaag tttctgaaat
     1261 cttgcgttgg ccggcgcggg aattcaaatt tatgattccc gttcgcatgg acgatagcgc
     1321 aatcaaagtc ttttttggga tgcgcgtcca acattccgat gcccgtgggc catccaaagg
     1381 tggcatccgc ttccatcctt ccgagactct ggataccgtc cgggcactcg ccatgtggat
     1441 gacctggaaa tgcgcggtgg ccgatatccc tctggggggt ggcaaaggtg gcgtggccgt
     1501 tgatccggca accctttcga tctctgagca agaaagactc tgccgcggct ggatcgacca
     1561 gatctggcgc aatatcggtc cccgccagga tgtccctgct ccggacgtcg gcacgaaccc
     1621 acggatgatg ggttggatga tggacgaata ttccaaactg gtcgggcagt acacgccggg
     1681 cgtgatcact ggcaagccgg tcggtggcgg cggctcactc ggtcgcaccg aggctactgg
     1741 tttcggcgtt atttacaatg tgcgcgaggc aatgaaacac ttaaagatcg attcgaccaa
     1801 atcggtcgcc gcgatccagg gtttcgggaa tgtctcccaa tatgcagcca taggcttttc
     1861 tgagctgctg gggggcaagg tcgcctgcgt ctcctattgg gataggcagg atcgcacctc
     1921 ttacaccgtc ctgcaccccg aggggatcaa tccacgcttc ctccagacca ttaccaatac
     1981 ctatggcgcc atcgacaagg atgctgcagt caaggctggc tatcgcatcg cctccggtga
     2041 tgcctggatt gagcaggatg tggatgtcct catccctgcc gccctggaag gtcagatcaa
     2101 tgctgagacg gttaaacacg tcagcgaccg cgtcaaactg gtcgctgaag gcgccaacgg
     2161 ccccaccaca cctgaagcgg acgcggtttt caagcagaat aatgtctttg ttatccccga
     2221 cttcctgtgc aatgctggcg gcgtgacagt ctcctacttc gaaagcgtcc aaaatgacat
     2281 gaatttctac tggacaagcg aagaagtcct gcagcgattg gatacaaaaa tcactcaggc
     2341 tttcgatagt gtggtcaaga tggctgaatc tgaaaaggtt ttcatgcgcg atgctgctta
     2401 catggtcgcc attgacaagg tcgtcaaagc tatggagctg cgcggcttga tctaaggccg
     2461 gtaaatttcc gcccattaac aacccggcgt cgcctgaccc gggttgtttt atat
//
LOCUS       DATMXK010000380         6607 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_43002, whole genome shotgun sequence.
ACCESSION   DATMXK010000380 DATMXK010000000
VERSION     DATMXK010000380.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6607)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6607)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6607
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43002"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..368)
                     /locus_tag="VN363_07060"
     CDS             complement(<1..368)
                     /locus_tag="VN363_07060"
                     /inference="COORDINATES: protein motif:HMM:NF015581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF302 domain-containing protein"
                     /protein_id="HWQ84307.1"
                     /translation="MEEMGFEVHLDLPYDAAVERVVAALKSEGFGVLTRIDVKATMKE
                     KLDQDFRPYVILGACNPPLAFKALSTDPLMGLMLPCNVTVEADPAGGSLARLVNPETM
                     LATGNLRENENLRAVAEEARA"
     gene            complement(373..1140)
                     /locus_tag="VN363_07065"
     CDS             complement(373..1140)
                     /locus_tag="VN363_07065"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HWQ84308.1"
                     /translation="MLPILQIGPLAIQTPGLILLLGLWLALTISERAAARNGLDASQL
                     YNLVLIALVAGLMGARLGYVLRYPQAFAESPASILSLNPGLLELWGGAAGAVLAALIY
                     GQRKGLPAWPTLDALAPGMAVLGVALGFSHLASGAAFGAPTSLPWGIELWGAARHPSQ
                     VYEIIAAALILGVVWPGQKISGQLNAAAPGLTFWVFSALSALSRLILEAWRGDSEIIL
                     GSLRQPQLIAWLVLALSLWAIGRIFTQSRSTIQTMEG"
     gene            complement(1153..1749)
                     /locus_tag="VN363_07070"
     CDS             complement(1153..1749)
                     /locus_tag="VN363_07070"
                     /inference="COORDINATES: protein motif:HMM:NF020123.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlpA disulfide reductase family protein"
                     /protein_id="HWQ84309.1"
                     /translation="MDSEQFPARSHTFPSWPGRGLWNLFSLAVLLLGAGWIWLSAANP
                     GGTTGGEIPAPRAGFLAPDFSLPNADGNLITLSDLRGQAVLVNLWASWCAPCQAEMPA
                     MQRVYKDYGDQSFTILAVNATNQDSQAAALQFAAEKNLTFPILFDLDGSVSRSYQLTA
                     LPTSYFILPDGTIKEVIIGGPMAEALLRVRVEQILEAR"
     gene            2064..2456
                     /locus_tag="VN363_07075"
     CDS             2064..2456
                     /locus_tag="VN363_07075"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84310.1"
                     /translation="MLSVLPKKFRAQSLLAGNLLTIKTLAVSIFAVSLLAGCASAAPA
                     TSTSGSLLPPAGSPSYPPAGEVSPLPIQELPTPESQPAAASAPTQVFTATPPSSRKEV
                     KAGLEATDPTTVQLASGEVQLVEFFAFW"
     gene            2472..2696
                     /locus_tag="VN363_07080"
     CDS             2472..2696
                     /locus_tag="VN363_07080"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84311.1"
                     /translation="MAPIVHGLEAEYSERMNFIYLDIDDPDTDPFKSTLGYRIQPHIF
                     LLDEHGKIIGTWLGSVTEAELRQAFDSALE"
     gene            2757..3257
                     /locus_tag="VN363_07085"
     CDS             2757..3257
                     /locus_tag="VN363_07085"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84312.1"
                     /translation="MLAEFGERTFVENFAAQNTPENMSVYLAGAFNPDKQAAELADPG
                     SVFLLAEVEGELAGYAHLHAGPPTLETLDQTGIDSDARDAIELVRIYADTPWIGKGVG
                     PALMQACLDESRSRGFELIWLGVWEHNPRAQVFYRKWGFEKIGTHIFMLGADPQTDWI
                     MAREIK"
     gene            complement(3250..4227)
                     /locus_tag="VN363_07090"
     CDS             complement(3250..4227)
                     /locus_tag="VN363_07090"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560649.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine-5'-phosphate glycosidase"
                     /protein_id="HWQ84313.1"
                     /translation="MKSKIQSLPIHMSTEARRALKQGQPVVALESTVITHGLPHPENL
                     ALARDLEAEVRAYGATPATIAVLEGRVYVGLEDELLESLGDATRLSGGLASDLHKISS
                     RDIGTAVAKGWSGGTTVAATLAIAERAGIQVFATGGIGGVHRTAPFEQAFDISTDLVL
                     LGRTPVIVVCAGAKAILDLPATLEVLETYGVPVVGFQTDDFPAFYSVSSGLQVSARTD
                     SPAEIAALARAHWGMGQPSAVLVGNPPPEEDALAPEEINGIIERALAELQAQGIRGQA
                     VSPFLLARVSELSEGASLHANLGLLKNNARLAAQVAVELSREGVRPLSI"
     gene            complement(4229..5179)
                     /locus_tag="VN363_07095"
     CDS             complement(4229..5179)
                     /locus_tag="VN363_07095"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbohydrate kinase family protein"
                     /protein_id="HWQ84314.1"
                     /translation="MADYAIPNPESPVLLIGAAGVDILGRLRGELQDSSNPARIRFAY
                     GGVVRNIAENLARLGQETVLLTAVGSDETGDGLLEDAAEAGVDTSRVLRVNDYPTGSY
                     IGVVGPRGDIQLALDDMRIISQVTPEYLEENAALFKEASLLVVDTNLSKETLRKSFSL
                     ARSAHLPVIADPTSISLTQRLYPYLKRIFMVVPNELEAGLLCDRELSPGKRHQAMEAA
                     QHLVALGVQIAIITMSKMGVVYATSTTSGHIRALRSEIVDPTGAGDALTAAVIFGLLN
                     NFPLDDALHMGVSAASMALRYQGASIPDLSLDKLYEQLVI"
     gene            5388..>6607
                     /locus_tag="VN363_07100"
     CDS             5388..>6607
                     /locus_tag="VN363_07100"
                     /inference="COORDINATES: protein motif:HMM:NF015948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF362 domain-containing protein"
                     /protein_id="HWQ84315.1"
                     /translation="MTDFPGKGKVAVLKTSPDTVIEDYARLMKLAGVEAALPKSIQTG
                     LKINVSWQTWYPACSSTPWQLEGVIRGLRGLGYSDLVGVHNDTVVVNTADAEVNNKHR
                     FVTDMLGVPCIYLYNQEFEWIEYQPQKPFLVLDKVYPEGVFIPKALVGMNIVQLPTVK
                     THVFTTITGAMKNAFGGLLHRNRHYTHAYIHDTLVDLLIIQQEIHPGIFAVMDGTFAG
                     DGPGPRAMRWHEKDILLASADQVAIDAVSARLQGFDPLGIRFIRRAHELGLGVGDPRQ
                     IEIVGYDIEQESPWNFVQEDTFASRGQKLIYHGPLKPLEKPLLQSPLVPWSYFASNFY
                     HNVYWYPFVGRKRVEAALQTRWGQLFRSYGDGQVVMPGMEPRTVVQAGIGMAALSLGL
                     AGLAYLLRRKATAA"
BASE COUNT         1355 a         1965 c         1933 g         1354 t
ORIGIN      
        1 gccctggcct cttctgccac cgcgcgcaga ttctcgttct cccgcaggtt accggtcgcc
       61 agcatggttt ccggattgac caggcgcgcc agcgatccgc cagccggatc ggcttctacg
      121 gtcacattac acggcagcat caggcccatc agcgggtcgg tgctcaaggc tttgaatgcc
      181 aggggcgggt tacaggctcc taaaatgaca tacgggcgga agtcctgatc cagtttttct
      241 ttcatcgttg ctttcacatc gatgcgcgta agcacgccaa acccttctga tttcagcgct
      301 gccaccacac gttccacggc agcgtcatac ggcaggtcca ggtggacctc aaaacccatt
      361 tcttccatag ctttatccct ccattgtttg aattgtacta cgtgattgag tgaatattcg
      421 cccgatcgcc cacaggctga gcgccagaac cagccaggcg atgagctgcg gctggcgcaa
      481 acttcccagg atgatctcac tgtctccccg ccaggcttcc aggatcaatc gactcagggc
      541 gctgagcgcg gaaaataccc aaaaagtgag cccgggtgcc gcagcgttca actgcccgga
      601 tattttttga ccaggccaga ccactcccag gatcaaagct gcggcgatga tctcatacac
      661 ctgagatgga tggcgggctg ctccccacag ctcaatcccc cacggcaggc tggtgggagc
      721 gccgaaagcc gcgccagacg ccaggtgcga aaaacccagc gccacgccca gcacagccat
      781 tcccggtgcc agcgcatcca gggtaggcca tgccggcagt cctttgcgct gcccatagat
      841 cagagcggcc aggactgcac cggcagcacc gccccagagt tccagcaagc cggggttgag
      901 ggagagaatg ctggccggac tttctgcgaa ggcctgcgga taacgcagca cataccccaa
      961 ccgcgctccc atcaacccgg ctacgagggc gatcaagacc agattgtaaa gctggctggc
     1021 gtccaggcca ttgcgggcag ccgcacgctc gctgatcgtg agcgccagcc acagcccgag
     1081 cagcaggatc aggccagggg tttgaattgc caggggaccg atttgcagga tagggagcat
     1141 ggtccgccgc gttcagcgcg cctccaggat ttgctcgaca cgcacccgca gcaaagcttc
     1201 tgccatcggc ccaccgataa tgacctcttt gatcgtacca tcgggtaaga tgaagtagga
     1261 cgtgggcaaa gcagtcagct ggtagctgcg cgaaacgctg ccgtccagat cgaacaggat
     1321 tgggaaagtc aggttctttt cagcagcaaa ttgaagggcc gcagcctggc tgtcctggtt
     1381 ggtagcgttt acagccagga tcgtgaagct ctggtcgccg taatccttgt agacccgctg
     1441 catggcgggc atctcagcct ggcagggcgc gcaccagctc gcccacaggt tcaccaggac
     1501 ggcctgtcca cgcaggtcgg aaagggtaat taaattacca tctgcatttg gcaggctgaa
     1561 atcaggcgcc agaaaacctg cccgtggggc gggaatctcg ccgccggtag tccccccggg
     1621 gtttgcggcg ctcagccaga tccagcccgc acccagcagc aggacggcca gactgaatag
     1681 attccagagt ccgcggccgg gccaggaggg gaatgtatgg gatcgcgcag gaaattgttc
     1741 gctgtccatc ttttcttaga tgcattccag tggataaggt cgcatgccag ttcagtcgct
     1801 cagaatgagc cctacacgga agtctcgttg ctggtttgca gggtggttcg gattgagaat
     1861 aacctcagcg gcagctttgt gtcaatccgt cgtgatgaag gtagcgctaa ggctgtgaat
     1921 tggcttccag ggacaatgat cagaaagatt gttcagcatg agaaccggat gatagtttgc
     1981 ttccagcggg aggtggattc ttgacaatta aaatgtccat ggtagtattt tcctatctat
     2041 tatcggtatt caggagcatt catttgctat ccgtccttcc caagaagttt agagcgcaaa
     2101 gcctgctggc aggtaatctg cttactataa aaactctggc agtcagcata tttgcagtca
     2161 gcctgctggc tggctgtgcg agcgctgcgc cagccacttc aacctccggc agcctgctgc
     2221 cgcctgcggg cagcccgtct tacccgcctg ccggtgaggt cagcccactg ccaatccagg
     2281 agttgcctac cccggagagc cagccagcgg ctgcatccgc accgacccag gtgttcacag
     2341 ccacgccgcc cagctcacgt aaagaagtca aagctggcct ggaagccact gatcccacca
     2401 ccgtccagct cgcatcaggc gaggtgcagc tggttgaatt cttcgctttc tggtgaggga
     2461 cctgccgtaa aatggcgccc atcgtgcatg ggctggaagc agaatatagt gagcgcatga
     2521 atttcattta cctggatatt gacgatccgg ataccgaccc ttttaaatca accctgggtt
     2581 accgcatcca accgcatatt ttcctgcttg acgagcatgg caagatcatt ggcacctggc
     2641 tggggtcggt gactgaagcc gagctgcgcc aggcatttga ctcagctctg gaataatccg
     2701 cgctgaacca atcccacatc atcaccaccc ggacagcccg tccggaagaa aatatcttgc
     2761 tggctgaatt cggggagcgc acattcgttg agaattttgc cgcccagaat acgcctgaaa
     2821 atatgtctgt ctatctggcg ggagccttca atccagataa gcaggctgct gagctggctg
     2881 atccggggag cgttttcttg ctggcggaag tggaaggcga gctggcgggt tatgctcatc
     2941 tgcacgccgg tccgcctacc ctggaaaccc ttgaccagac cgggattgat tcggatgctc
     3001 gggatgccat cgagctggtg cgcatctatg cagatactcc ctggatcggc aaaggcgtcg
     3061 gaccagccct gatgcaagcc tgcctggatg aatcccgcag ccggggcttt gaattgatct
     3121 ggctgggagt gtgggagcat aacccgcgcg cccaggtgtt ctatcggaag tgggggtttg
     3181 aaaagatcgg gacgcacatc ttcatgctgg gcgcagaccc tcaaaccgac tggatcatgg
     3241 cccgcgaaat taaatagaca gcggtctgac tccctcccgg cttaattcca ctgccacctg
     3301 ggctgccagg cgggcattat tttttaatag acccagattg gcgtgcaggc tggcgccttc
     3361 gctcagctcg ctgacccgcg ccagcaggaa cggggaaact gcctggccgc ggatcccctg
     3421 ggcttgcagc tctgccaggg cgcgctcgat tatgccgttg atctcctcgg gagccagagc
     3481 gtcttcttca ggaggcgggt tgcccaccag cacagcgctg ggctggccca tcccccagtg
     3541 ggcgcgggcc agtgcggcaa tctcagccgg gctgtcggtg cgggcgctca cctgcaagcc
     3601 gctgctgacc gaatagaaag ccggaaaatc gtcggtctgg aatcccacca ctggaacgcc
     3661 ataggtctcc agtacttcca gtgtggcagg caggtctagg atcgctttgg cgccggcgca
     3721 gaccacgatc accggcgtcc gccccagcag caccagatcg gtagatatat caaaagcctg
     3781 ctcgaaggga gccgtgcggt gcacgcctcc gatcccgccg gtagcaaaga cctggatacc
     3841 ggcgcgttcg gcgatcgcca gggtcgccgc tacggtcgtc cctccgctcc agcccttggc
     3901 taccgcggta ccgatatcgc ggctggaaat cttgtgcaga tcgctggcca gcccaccgga
     3961 aaggcgggtg gcatcgccca ggctttccaa gagctcgtcc tccaatccca catacacccg
     4021 tccctccagc acggcgatcg tcgccggcgt tgcgccgtag gcgcgcactt cggcttccag
     4081 atcccgcgcc agagccaggt tttcaggatg gggcaggccg tgggtaatga cagttgattc
     4141 gagggctacg actggctgtc cttgcttcag ggcgcggcgg gcttccgtag acatatggat
     4201 cggcaggctt tggattttgg atttcatttt agatcaccag ttgttcgtag agtttatcca
     4261 gggatagatc tggaatgctg gcgccctgat agcgcagcgc catcgaagcg gccgagacgc
     4321 ccatgtgcag tgcatcatcc agtgggaaat tattgagcag gccgaaaatc accgctgcgg
     4381 tcagggcgtc gccggccccg gtgggatcaa cgatctcgct gcgcagggcg cgaatatgcc
     4441 cgctggtggt ggaagtggcg tacaccacgc ccattttcga catggtgatg atggcgatct
     4501 gtacacccag ggcgaccagg tgctgggcgg cttccatcgc ctggtggcgc ttgccgggag
     4561 ataactcccg gtcgcacagc agcccggctt ccagctcatt tggcacgacc ataaatattc
     4621 ttttcaggta tgggtaaagg cgctgggtga ggctgatcga ggtcggatcg gcgatcaccg
     4681 gcaggtgcgc actccgggcc aggctgaagg acttgcgcag ggtctctttg gacaggttgg
     4741 tatccactac cagcagtgaa gcttccttaa atagtgccgc attctcctcc aggtattccg
     4801 gtgtcacctg gctgatgatg cgcatatcgt ccagcgccag ctggatatcg ccgcggggtc
     4861 ccaccacacc gatgtacgat ccggtggggt agtcgttaac ccgcagcacc cggctggtat
     4921 ccactcctgc ctcagccgca tcctccagca gcccatcgcc ggtctcgtcg ctgcccaccg
     4981 cggtgagcag gacggtctcc tgtcccaggc gagccaggtt ctcggcgata ttgcgcacca
     5041 ccccgccata ggcaaagcga atgcgcgccg ggtttgagct gtcttgcagc tcgcctcgca
     5101 agcgacccag aatatcgacg ccggctgccc cgatcagcag gacgggagat tccggattag
     5161 gaattgcata atctgccatg gctgctccac tcaggatttt cgaccgcgct tgatgacctt
     5221 tattctagcc gagattaacc ggaatcaaca ggtgatctct gagaatcaat tgggatgtct
     5281 atccggccgc ctgcggctta caggattatc aaccccaaag ctactcacct ggcaaaaagc
     5341 ttgctacaat tgagtagcca tcaggcttta taaaatggaa ggtggttatg actgattttc
     5401 ccggtaaagg taaagtagcg gtgctgaaaa ccagccccga caccgtgatt gaagattatg
     5461 cccggttgat gaagctggca ggtgtggaag ctgccctgcc aaaatcgatc cagaccggct
     5521 tgaaaattaa tgtctcctgg cagacatggt atccggcctg ctcctcgacc ccctggcagc
     5581 tggagggggt gatccgcggg ctgcgcggcc tgggctactc cgacctggtg ggcgtgcaca
     5641 acgacacggt ggtggtgaat accgccgacg ccgaagtgaa caataaacac cgctttgtta
     5701 cggatatgct gggtgtgccg tgcatctatc tctataacca ggaattcgag tggatcgaat
     5761 accagcccca gaaacctttc cttgttctgg acaaggtcta ccccgaaggc gtatttatcc
     5821 cgaaggccct cgtggggatg aatattgtcc agttgcctac ggttaagacg catgtcttta
     5881 ccaccattac cggggcgatg aagaacgctt tcggtgggct actgcaccgc aaccgccact
     5941 acacccacgc ttacatccac gataccctgg tggacctgct catcatccag caggagatcc
     6001 atcccggcat ttttgcggtg atggacggca ccttcgccgg ggatggacct gggccgcgcg
     6061 ccatgcgctg gcacgagaag gatattctgc tcgcctctgc cgaccaggtt gccatcgacg
     6121 ccgtctcggc ccgcctgcag ggtttcgacc cgctgggaat ccgcttcatc cgccgggcgc
     6181 acgagctggg cctgggggtg ggcgacccgc gccagatcga gattgtggga tacgatatcg
     6241 agcaggaaag cccctggaat ttcgtccagg aggatacttt tgccagccgc ggccaaaagc
     6301 tgatttacca cggcccgctc aagccgctgg aaaagccctt gctgcaaagt ccgctggtgc
     6361 cctggtcgta tttcgccagc aatttctacc acaacgtcta ctggtatccc tttgtggggc
     6421 gcaagcgagt ggaagccgcc ctgcaaaccc ggtggggtca attattcagg tcgtatgggg
     6481 acgggcaggt ggttatgccg ggcatggagc ccaggaccgt agttcaggcc gggattggga
     6541 tggctgcgct ttcacttggt ctggcgggat tagcctatct gctgcgccgc aaagccacag
     6601 ccgctaa
//
LOCUS       DATMXK010000381         3614 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_109666, whole genome shotgun sequence.
ACCESSION   DATMXK010000381 DATMXK010000000
VERSION     DATMXK010000381.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3614)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3614)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3614
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109666"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..719
                     /locus_tag="VN363_07105"
     CDS             <1..719
                     /locus_tag="VN363_07105"
                     /inference="COORDINATES: protein motif:HMM:NF013353.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0036361 - racemase activity, acting on
                     amino acids and derivatives [Evidence IEA];
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="aspartate/glutamate racemase family protein"
                     /protein_id="HWQ84316.1"
                     /translation="YRPDDWLEETEAQYASYARPGTQLRAAAITAGPQTIETHYLKAM
                     SVPGMLRLARQAERAGATALVIDCMSDPSLDAAREAVSIPVVGPAQTSAFLAASLAHR
                     FSILGTRADMGHKFVNQINEYGLGSKLASVQTVGLSVQQVESDPEAMFKGLLQAGERA
                     IVEDGAHILIPGCTGMIGIAGKLQAALEARGHCVPVIEGPAAAVKMAEALSDLGLAQS
                     KLSYPTPPHKQLAGYEDLEF"
     gene            759..1286
                     /locus_tag="VN363_07110"
     CDS             759..1286
                     /locus_tag="VN363_07110"
                     /inference="COORDINATES: protein motif:HMM:NF025865.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase family protein"
                     /protein_id="HWQ84317.1"
                     /translation="MDTLEAIFSRRSIRAFTAESVSDEALQTIIQAAAAAPSGSNLEK
                     RIFLSIQKPRRIAALRALAPGIIGRPAAVIVICLDQRSDPGEKQAGIDLSQFMNIGAA
                     LQNLLLAAHASGLGACPVASFHAQGLGIFLGLPEGIVPCLLVVLGKPRFTPSAPRKRP
                     LDEIYYRETFEISHG"
     gene            1279..1584
                     /locus_tag="VN363_07115"
     CDS             1279..1584
                     /locus_tag="VN363_07115"
                     /inference="COORDINATES: protein motif:HMM:NF039991.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6092 family protein"
                     /protein_id="HWQ84318.1"
                     /translation="MDSPTFNTQEELFNLVAYLITSARGLYDEPPDYGSFRLLDASGR
                     LLEIMSTTGDLDPFLARLKEEIDAEREGSMDNDRQRQNLERWVIEIAQELRHRTGTD"
     gene            1764..2909
                     /locus_tag="VN363_07120"
     CDS             1764..2909
                     /locus_tag="VN363_07120"
                     /inference="COORDINATES: protein motif:HMM:NF044937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HWQ84319.1"
                     /translation="MPTRTIKTAQDCADFLTGLKLMGTGGGGSPTTGLELLSKALAEG
                     LELSWIDAADLPDEVFSCTTFGSGSISAGRPESEAEITRLGEKLGLENKFGFRAPEMA
                     VRELAAYTGTKIGALVAVELGASNTPAPLVTAARMGIPLVDGDYSGRAVPEDMQTTYF
                     LKDIQTYPAAITDWWGDVLILKEASATQMGERMGKMLAIASHGVVFIASMLLSAKDTR
                     ETVVPGTLTLSFELGKAVRQARAAGRDPIHAAAQALEGWELFRGEVTGKEWQDKEGVM
                     VGTTHISGSGAWAGHTLDVWFLNENHIAWLDGAPYVFSPDLIILADPASGEGYTNTEI
                     KAGDPVAVLGAKVYPAFRSQKGIQFFGPRYWGFDIDYVPIEDVVGKG"
     gene            complement(2969..>3614)
                     /locus_tag="VN363_07125"
     CDS             complement(2969..>3614)
                     /locus_tag="VN363_07125"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ84320.1"
                     /translation="TNIANLLSNLGILARYQRDDIEARRLYLESLSIRRTINDRWGIS
                     VSLNNLGIHAMDQGELGEARTFLEEAVALQREIGDQYYLANYLNNLGNVARAQGDYIA
                     AGPLYKESLERNRDLGDGWQIAYVLEDIGGLAALSGQPQRALTLWGAAAALREQIGAP
                     LAPHELTRLEKFIHTAHAALESQQGDTAWQSGRGMSMDEAIALALEKSRLSPTP"
BASE COUNT          759 a         1011 c         1067 g          777 t
ORIGIN      
        1 gctaccgccc ggatgattgg ctggaagaga ctgaggccca gtatgcatct tatgcccggc
       61 cgggtacaca gttgagggca gcagccataa ctgctggccc acaaaccatc gaaactcatt
      121 atctcaaagc gatgtctgtg cccggtatgc tgcgcctggc ccgccaggct gagcgcgccg
      181 gcgccaccgc tctggtaatc gattgcatga gcgatcccag tctggacgcg gctcgcgaag
      241 ccgtttctat ccctgtggtt ggccctgccc agacctctgc attcctggca gcttcgctgg
      301 cgcatcgttt ctcaatcctg ggaacgcgcg ccgacatggg tcacaaattc gtcaaccaga
      361 tcaatgagta tgggttgggg agcaagctgg cttcggtcca gactgtggga ttgagcgtgc
      421 agcaggtgga aagcgatccc gaggccatgt ttaagggctt gctgcaagcc ggcgagcggg
      481 ctatcgttga ggacggtgct cacattttga tcccgggctg taccgggatg atcggtatcg
      541 ctggcaagct gcaggcagct ctggaagcgc gcggccactg cgttccggtg atcgaaggcc
      601 ccgcagctgc agtcaagatg gctgaagcgt tgagcgacct gggattggcg cagagcaagc
      661 tcagttatcc cactccgcct cacaaacaat tagccggcta tgaagattta gagttttagt
      721 attccataat tgcctgaata gaaactctgg agcaagattt ggataccctg gaagcgattt
      781 tcagccgccg cagcatacgc gccttcaccg ccgaatctgt gagtgatgaa gccctgcaaa
      841 cgattatcca ggcggcggca gccgcgccga gcggcagtaa cctggaaaaa cgcatcttcc
      901 tttcgatcca gaagccgcgg cggatcgctg ccctgcgcgc cctggcgccg gggatcatcg
      961 gtcgcccggc agcggtaatc gtgatctgtc tggaccagcg ctccgatccc ggcgagaagc
     1021 aagctgggat tgatctcagc cagtttatga atatcggtgc ggcgctgcag aatctgctgc
     1081 tggcagcgca tgcctcaggg ttgggggctt gcccggtggc atcctttcat gctcagggat
     1141 tgggtatctt cctggggctg ccggaaggga ttgtcccgtg cctgctggtt gtactcggca
     1201 aaccgcgctt cactccctct gccccgcgca agcgccctct cgatgagatc tattaccgag
     1261 aaacctttga gatctcccat ggatagccct actttcaaca cacaggaaga acttttcaac
     1321 ctggttgctt atctgatcac cagcgcccgg gggttatacg atgagccgcc tgattacggc
     1381 tctttccggc tgctggatgc ttccggcagg ctgctggaga tcatgagtac caccggtgac
     1441 ttagacccct tcctggctcg cttgaaggaa gagatcgacg ccgagcgtga gggcagcatg
     1501 gacaatgatc gccagcgcca aaatctggag cgctgggtta tcgagatcgc gcaggaatta
     1561 cgccaccgca ctggaaccga ctgaatattg ggtgattgaa ttagcgcagg aattacgcaa
     1621 ccgcgcgaga accggctgaa tatcaggtta ttgaaatagc tcaggaatta cgccaccgcg
     1681 tgggacccga tcgaataccc gattgttgaa attacgcaag agctgcacct gtgatagtaa
     1741 acaaatcaaa gtaaggagac cttatgccaa cccgaaccat taaaactgct caagactgtg
     1801 ccgatttcct caccgggctc aaactgatgg gcaccggcgg aggcggctct cccacgaccg
     1861 ggctggagct gctcagcaaa gccctggctg aaggcctgga gctgagctgg atcgatgctg
     1921 cggatttgcc tgatgaagta ttttcttgca cgacctttgg ctccgggtca atctctgccg
     1981 gacggcctga gagcgaagca gagatcaccc ggctgggcga aaaactggga ttggagaaca
     2041 aattcggctt tcgggctcct gagatggcag tccgcgagct ggctgcttac acagggacga
     2101 agatcggtgc gctggtggca gtggagcttg gcgcaagcaa cacccctgca ccgctggtga
     2161 cagccgcccg gatgggtatc ccgctggtgg acggcgatta ctccggtcgg gcagtccccg
     2221 aagatatgca aaccacttac ttcctgaaag atatccagac ttatccagcg gctattaccg
     2281 actggtgggg ggatgttttg atcttgaagg aggcttccgc aacccagatg ggcgaacgga
     2341 tgggcaaaat gctggcgatc gccagccacg gtgtggtttt cattgcctcc atgctgctat
     2401 cagccaaaga cacccgcgag accgtggtac ccggcactct gaccctcagc ttcgagctgg
     2461 gtaaggccgt gcgccaggcg cgcgcagccg ggcgcgaccc tatccacgct gcggcccagg
     2521 cgctggaggg atgggagctc ttccgcggtg aagtgactgg caaggagtgg caggataaag
     2581 agggtgtgat ggtgggtaca acccatattt caggcagcgg tgcctgggcc gggcataccc
     2641 tggatgtgtg gttcctgaat gaaaaccaca tagcctggct ggatggtgcc ccttatgtgt
     2701 tcagcccgga cctgatcatc ctggccgatc cagccagcgg agaagggtac acaaataccg
     2761 agatcaaagc tggcgacccc gtggcagttc tgggtgccaa agtctatccg gccttccgga
     2821 gccaaaaggg gatccagttc ttcggcccgc gctactgggg ctttgatatc gattatgtgc
     2881 cgatcgaaga cgtggttggc aagggatagg ctcctcgcgt aaaatagcga ctttttctat
     2941 cgtgagcatg ttgggaattc ctccttgatc atggcgtcgg cgataatctt gacttttcca
     3001 gggccagggc gattgcttcg tccattgaca tacccctccc tgactgccag gcagtatctc
     3061 cctgctggct ttctaaagca gcgtgagctg tgtggataaa tttttccagg cgggttaatt
     3121 cgtgcggggc aagcggtgca ccaatttgct ccctcagcgc tgcagcagct ccccagaggg
     3181 tcaatgcccg ctgaggttgc cctgagaggg cagctaatcc gccgatatcc tccagaacat
     3241 acgcgatctg ccagccatcc cccaggtctc gattacgctc caggctttct ttatacagcg
     3301 ggccggctgc tatataatca ccctgagccc gggcgacatt tcccaggtta ttaaggtagt
     3361 tagccaggta gtactggtcg ccgatctcgc gttggagggc cactgcttct tcaagaaaag
     3421 tgcgcgcctc gccgagctcg ccctgatcca tggcgtggat accgagatta ttgagcgata
     3481 cactgatccc ccaacgatca ttaattgtcc tgcggatgga gagactttcc agataaagcc
     3541 ttcgggcttc gatatcatcc cgctggtagc gcgccaggat tcccaggttg ctcaataaat
     3601 tggcgatgtt ggtc
//
LOCUS       DATMXK010000382         6429 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_93496, whole genome shotgun sequence.
ACCESSION   DATMXK010000382 DATMXK010000000
VERSION     DATMXK010000382.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6429)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6429)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6429
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_93496"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(112..1842)
                     /locus_tag="VN363_07130"
     CDS             complement(112..1842)
                     /locus_tag="VN363_07130"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWQ84321.1"
                     /translation="MTDVSGTIAENLAASFSSVPEQTALVLLHSSQPEQIIHYRDLES
                     GSSAYAQLLERAGIASGEVVILILPHGKDLIFSFFGTILHGAVPSIMPFLTEKLSADQ
                     YRASLAALFEITAPAAVITYPEFVSEVNQAASEAASIRAVLVSNQVADHSANSSRDFQ
                     GLQRSPSDIALLQHSSGTTGLQKGVALSHTAIFNQLTHYSEAIQLTSQDVIVSWLPLY
                     HDMGLISSFLMPILLRCPLILISPFDWVRAPVRLLQAVSTYHGTLTWLPNFAYNFCSQ
                     KIRSRELEGVDLSSWRAVINCSEPMHRKSHSMFLERFQAFGLKPSALATCYAMAENVF
                     AVTQGGINQTVKIDHINTATYTSQQIAEQVAPDHPQALPMLSAGRPISGTNLRVVDPQ
                     GKDLPERQIGELAIHSDCMLSGYYRRPDLTEKAFMDGWYLTGDLGYLAGGEVYVTGRK
                     KDLIIVGGKNIYPQDIERLVNEVPGVHPGRVAAFGIFDEQAGTEEVVVIAEMDQSKQS
                     SQNDLLDVWDQAAGQLAAEIRQKVNHGSDIVLRAAHIVEKDWLIKTSSGKIARSANRT
                     KYLAEMAEEN"
     gene            complement(1842..2093)
                     /locus_tag="VN363_07135"
     CDS             complement(1842..2093)
                     /locus_tag="VN363_07135"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWQ84322.1"
                     /translation="MADQIIPQLKEYIATQILRQPHYVIGDDDRLLTSGLIDSFHLVD
                     LSIYIEEQYGVRIDDTELNSDVFDTLRQLAVVIKSRQNR"
     gene            complement(2103..2960)
                     /locus_tag="VN363_07140"
     CDS             complement(2103..2960)
                     /locus_tag="VN363_07140"
                     /EC_number="3.1.-.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016788 - hydrolase activity, acting on
                     ester bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="SGNH/GDSL hydrolase family protein"
                     /protein_id="HWQ84323.1"
                     /translation="MSIPSDSPSAVSPTLPTESSTPTFTAIPPLSADEWQTLPVIPEV
                     FSRVKEIYTRGQSLGNNPNAFSKIGDCGSTPAWFLGDFDRGPRFYALGEYTELDAVIQ
                     AFQGSYERTSLAAKAGFNASSVFSLIWSDRTQCNANETPLACEYRVHNPAYAFITLGS
                     NDVYHQDTFEKQLRAIIEFSIEQGVIPILSTKADNLEGDGSLNAQIAQLAHEYEVPLW
                     NYWLAVQSLPDQGLQEDQVHLTWGPNQFDDSQAMKSAWTIRNLTALQSLDIVWRSVTS
                     QLEAQTLKP"
     gene            complement(3178..4317)
                     /locus_tag="VN363_07145"
     CDS             complement(3178..4317)
                     /locus_tag="VN363_07145"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84324.1"
                     /translation="MSSSIDHEYSSRFWVRVILKALFLFLGLNLLFGILDPMQFLGKL
                     TIYNSFIPGRVRLPYGENPASAYNLSLYNLPAMFASHELAGAQKPPDEYRVILIGDSS
                     TWGYLLAPDETLAAFINQQQLRLPDGRLVRAYNLGYPVMSVTKDLLILTHALNYQPDL
                     IVWPVTLESLPRSKQLFPPLLQNNPADVRKLIAAHDLNLDSNDPGFVEPDFYQKTLVG
                     QRKEIADWLRLQFYGILWAATRIDQELPDQYIPRQEDLSSDVSFHELQPPALSESALA
                     LDVLEAGMDLAGNIPVLLINEPMFISQGANSDLRYNFYYPRWAYDAYRELLLNLANKN
                     SWHYLDLWNSVDPTEFTNSAVHMSPLGTNQFAMQIGSEILRLQQP"
     gene            complement(4325..5767)
                     /locus_tag="VN363_07150"
     CDS             complement(4325..5767)
                     /locus_tag="VN363_07150"
                     /EC_number="2.3.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015049.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MBOAT family O-acyltransferase"
                     /protein_id="HWQ84325.1"
                     /translation="MTLTQILAFAGFSIGSVLFLPAAWRRKVWFMASLLAIYWLQPST
                     PIRNLDFWFPTVAILLTLLCWAATQNSGELLKQNRLALLLTGAAIAIVSLTRYFELLC
                     CLTPTRPPPFWQVGIFILTAAILILLICKRYYRRSWLLDTLALSLVLIFILLKSPILG
                     QTVSSVLRTQTGQAVELANASDLHWLGFSYLAFRLIHTLRDARAGRLPALELDEYLTY
                     ALFWSTYTSGPIDRLGRFSTDLRQAVSQRVSRIIDPHTPFWSGFWRIMWGIFKKFAVA
                     DTLALIALNETNASQVSSAGWMWLLLYAYSLRIYFDFSGYTDIAIGLGNLIGLKLPEN
                     FDRPYLKPNLTSFWNSWHITLAQWIRAYFTFPLTRTLRSRGGNLPVWMVIGITQVGTM
                     GLIGLWHGITWNFLIWGIWHGAGLFVHNRWLDRQRSRTEIVPASVLSQKFSQAVGWFL
                     TFQYVTLGWVWFALPSTPQAQLVFQQLLGL"
     gene            complement(5915..6310)
                     /locus_tag="VN363_07155"
     CDS             complement(5915..6310)
                     /locus_tag="VN363_07155"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84326.1"
                     /translation="MKRITILPGMFLITLFALTACSGSESSATAARGIQAYYEALVAK
                     DSDQLVNYSCADWEAQAQLELESFGAVKAELKDLQCSATGQDGDATIVSCSGVISAAY
                     GNEILEINLADRQYLAVYEGNEWRMCGYR"
BASE COUNT         1681 a         1571 c         1703 g         1474 t
ORIGIN      
        1 acctcaaacg gagctggacg gatccgcctg acaaccgatc cccggttgga ttttgatccc
       61 gtatggcgcc caatgccaac cccctgattt ttcgacgcag cctgaaatta attagttctc
      121 ttcagccatt tccgccaaat acttggtgcg atttgccgag cgggcaattt taccgctgct
      181 ggtttttatc agccagtctt tttcgacgat atgcgctgct cttaagacga tatctgagcc
      241 atgattgact ttctggcgga tttccgctgc caactgcccg gctgcctggt cccacacatc
      301 cagtaaatca ttctgactgg actgtttgga ttggtccatc tcggcgatta ccaccacttc
      361 ctcggtccca gcttgctcat cgaagattcc aaatgctgcc actcgtccag gatgcacgcc
      421 gggtacttcg ttcaccaggc gctcgatatc ctgagggtat atatttttcc cacccacgat
      481 aatcagatcc ttcttccggc ctgtaacata aacttctccc ccggcaaggt atcccagatc
      541 tccagtgaga taccagccat ccatgaatgc cttttcggtc aggtctggac ggcgataata
      601 gccagaaagc atacagtcgc tgtggatcgc cagctcgccg atctggcgtt caggcaaatc
      661 cttgccctgg ggatctacga cccggagatt cgttcctgag attgggcgtc ccgctgagag
      721 catgggcaaa gcctggggat ggtccggagc aacctgctcg gcgatctgct gggatgtgta
      781 agtggcggtg ttgatatgat caatcttgac cgtctggttg atccctcctt gagtcaccgc
      841 aaatacattt tcagccatag cataacaggt cgccagcgcg ctgggcttca gtccaaaagc
      901 ttgaaaccgt tccagaaaca tggagtgact cttcctgtgc atgggctcag aacaattaat
      961 gactgcgcgc cagctgctta aatccacgcc ttccagctcg cgggagcgaa ttttctgaga
     1021 gcagaagttg taggcgaaat tgggcaacca ggtaagcgtt ccatgataag tggaaaccgc
     1081 ctggagcagg cggacgggcg cacgcaccca atcaaatgga gaaattaaaa tcagcggaca
     1141 gcgcagtaat atcggcatca agaagctgga aataagcccc atatcatgat agaggggcag
     1201 ccagctaacg atcacatcct ggctggttaa ctggattgct tccgagtagt gggtgagctg
     1261 gttaaatatc gccgtatgcg atagagccac ccctttttga agaccggtcg ttccggacga
     1321 atgctgcagc agggctatat cagatggcga gcgctgtaaa ccctggaaat cgcgacttga
     1381 gtttgctgag tgatctgcta cctgattcga aaccaataca gcccggatgg aagctgcctc
     1441 agaagcagcc tgattgacct cgctgacaaa ttcagggtac gttattacgg cggccggggc
     1501 ggtgatctca aacaaggctg ccaggctggc gcgatattgg tctgcagaga gtttttcggt
     1561 cagaaacggc atgatcgatg ggactgcccc atgtagaatc gtgccaaaaa agctgaaaat
     1621 taaatccttg ccgtggggca ggattaagat aaccacttcg ccggatgcga taccggctct
     1681 ctccagcagc tgcgcatagg cagaggaacc agattccaga tccctgtagt gaataatctg
     1741 ttcaggctga gaggaatgca gcaaaaccag ggctgtctgc tctgggaccg agctaaaact
     1801 ggctgccagg ttttcagcaa tcgtaccgga gacgtctgtc attatctatt ctggcgagat
     1861 ttaataacga cggcgagctg gcgcagtgta tcgaatacat ccgagtttaa ctcggtgtca
     1921 tcaatccgaa ccccatactg ctcctcaata tagatggata ggtcaaccag atggaatgaa
     1981 tcaatcaacc cactggttaa taaacgatca tcatcaccaa tgacgtagtg gggttggcgt
     2041 aagatttggg tagcgatata ttctttcagt tggggaataa tttgatcggc catgattccc
     2101 tttcaaggtt taagggtttg cgcttcgagc tggctggtta cagaccgcca gactatatcc
     2161 agagattgca gggccgtaag attgcgaatt gtccaggcag atttcattgc ctggctatca
     2221 tcgaactgat tgggtcccca ggtcaggtgg acctggtctt cctgaagccc ttggtccggg
     2281 agggactgaa cagccagcca gtaattccat aaaggtactt catattcgtg agccagctga
     2341 gcgatctggg cattcaaact gccatcaccc tcaagattat cagctttcgt gcttaagatc
     2401 gggatcactc cttgctcaat agaaaattca ataattgctc gcagctgttt ttcgaaggta
     2461 tcctgatggt aaacatcatt actgccaagg gtgatgaacg cgtaggcggg gttgtggacg
     2521 cggtattcgc acgccagcgg agtttcatta gcattacatt gggtgcggtc tgaccagatc
     2581 aacgaaaata ccgacgaggc attaaaacct gcctttgctg ccaggctggt gcgttcataa
     2641 gaaccctgaa acgcctggat aacagcatcc agctcagtgt attctcccag agcgtaaaaa
     2701 cgcggtccgc ggtcaaagtc gccaagaaac caggctggag tgctgccaca gtccccaatt
     2761 ttcgaaaaag catttggatt attccccaga gattgccccc gggtataaat ttctttaacc
     2821 ctggagaaaa cttcagggat gaccgggaga gtttgccatt catctgcact gaggggtgga
     2881 atggctgtaa atgttggtgt gctggattcg gtagggagag tgggagagac cgcgctcggc
     2941 gaatcagaag gaatgctcac agtttgggtc gatgctgcta tagttgtatt cacctggata
     3001 ggtgagccga tggaaacaga ccttgtagct gcgtcagtcg tggcaaccgg cgagggagtt
     3061 cctgaaggag ctggaagaca gctggacagg ctaaataata agaagacgga taggagttgt
     3121 tggcggcatt tcatttcagc ccgattatac ataggaaacc cggttacaac cggaaattca
     3181 aggctgctgg agccggagaa tctcacttcc gatctgcatg gcaaattgat tcgtccccaa
     3241 ggggctcatg tggacagcgc tgttggtaaa ttcggttgga tcaacactgt tccagaggtc
     3301 aagataatgc caactatttt tattggccag gttcagcaga agctcacggt aagcatcgta
     3361 cgcccagcgg ggataataaa aattgtagcg cagatcgctg tttgcacctt gactgatgaa
     3421 catgggttca ttgattaaaa gtacggggat atttccagca agatccattc ctgcttccaa
     3481 tacatccagt gccagcgcgg attcggatag tgccggtggt tggagttcat gaaagctaac
     3541 gtccgaggat agatcttcct ggcgcggaat atactggtcc ggaagttcct gatcgatcct
     3601 ggtagctgcc cacaagatgc catagaactg caagcgcagc caatcagcaa tttcttttcg
     3661 ctgacccacc agggtttttt ggtagaagtc tggttcaaca aaaccaggat cattggaatc
     3721 cagattcagg tcatgcgctg caatcagttt acggacgtcc gccgggttgt tttggagcag
     3781 gggaggaaat aattgtttgg agcgaggtag cgattctaat gtaaccggcc acacaatcaa
     3841 atccggctgg taattcaacg catgggtaag gatcagtaag tctttggtga ccgacatgac
     3901 cggataaccg aggttgtagg cgcgcaccag gcgtccatct ggcagccgaa gctgctgctg
     3961 gttgatgaat gctgccaggg tttcatccgg tgcaaggaga tacccccagg tggatgagtc
     4021 cccgatcaaa ataacccggt attcgtcggg tggtttttga gcacctgcca gctcatggga
     4081 ggcgaacatt gccgggagat tatataaact caagttgtat gcagaagccg gattttcccc
     4141 atagggtaag cgaacccgtc ctggaatgaa cgaattataa atagtcagtt tgccgagaaa
     4201 ctgcatggga tcgagaatcc caaataacag attcaaacct aaaaacagga acaaggcctt
     4261 aaggatgact cggacccaga agcgggatga gtattcgtgg tcgattgagg aggacataga
     4321 gcgtctataa ccccaataac tgctggaaaa ccagttgagc ctgaggagtg gatggcaggg
     4381 caaaccacac ccaacctaag gttacatatt gaaatgtaag gaaccatccc acagcctgtg
     4441 agaatttttg actgaggacg gaggctggaa ctatctctgt gcggctgcgt tgcctatcca
     4501 accagcgatt gtgaacaaat aaacctgctc catgccagat gccccagatc aggaagttcc
     4561 aggtaatccc atgccagagg ccgatcaagc ccattgtgcc gacctgtgtt atcccgataa
     4621 ccatccaaac ggggagatta cctcccctgc tgcgcagggt gcgggtgagg ggaaaggtga
     4681 aataagcccg gatccactgc gcgagggtaa tatgccagct gttccaaaaa gaagtaaggt
     4741 tcggtttgag ataagggcgg tcaaaatttt caggtagctt caggccgatc aggttgccca
     4801 gaccgatagc gatatcggta tagccagaaa aatcgaaata aattcgcaag ctatacgcgt
     4861 acagtaacag ccacatccag cctgctgagc tgacctggga ggcgtttgtt tcgttcagag
     4921 ctatcaatgc cagggtatct gccacggcaa atttcttgaa tatcccccac attatccgcc
     4981 aaaatcctga ccagaaagga gtgtgaggat cgattatccg tgagactcgc tggctcacag
     5041 cctggcgcaa atctgtgcta aaacgtccca gacggtcaat tgggcctgat gtgtaggttg
     5101 accagaataa agcgtaggtc aaatattcat ctaactccag agccggcagc cgcccggccc
     5161 gggcatcgcg cagtgtatgg atgaggcgga atgccagata agaaaagccc agccagtgca
     5221 gatcgctcgc attcgccagc tctacagcct gaccggtctg ggttcgtaac acggaactga
     5281 ctgtttgccc gaggataggt gattttagca ggataaagat cagaactaag gacagagcta
     5341 aagtgtccaa aagccaggaa cggcgataat atcgcttaca aatgagcagg atgagaattg
     5401 cagcagtgag gatgaatatc cccacctgcc agaatggagg tgggcgggta ggcgtcagac
     5461 aacaaagtag ttcaaaatac cgggtaaggc tgacaatagc aatagccgct cctgtcaata
     5521 acaaagccag ccggttttgt ttcaagagtt ccccgctgtt ttgagttgct gcccagcaaa
     5581 gaagggtcag cagtattgca actgtcggga accaaaagtc aaggttgcgg atcggggtgc
     5641 tgggctgcag ccaataaata gccaacaggc tggccatgaa ccataccttg cgccgccagg
     5701 ctgccggcag aaataataca gagccgatac tgaaaccggc gaaagccagg atttgtgtca
     5761 gagtcatgga atgaaattag aagccctggt agatccatgt cggagcctgg tcggaagtca
     5821 atatcaagat agcaatcacg atcaggcaca agagcagtgc atacaggttt tcaagcgaga
     5881 acagccaacg ccacatccga tcaatccccc aggtttagcg ataaccgcac atgcgccatt
     5941 cgtttccttc atagactgcc agatactggc gatcggcaag attgatctcg agaatctcat
     6001 tgccataggc agcggagata acgccgctgc aagacacgat ggtcgcatcc ccgtcctgcc
     6061 ctgtagctga acactgcagg tctttcagct cagcttttac agccccgaaa gattcaagct
     6121 ccagctgggc ctgggcttcc caatcggcgc aggaataatt caccagctgg tctgaatcct
     6181 tcgcaaccag ggcttcataa taagcctgga tacctcgtgc agccgtagca ctgctctcac
     6241 tgcctgagca agcggtcagc gcgaacaaag ttatgagaaa catacccggc aggatcgtga
     6301 tccgtttcat tccttagcac tccagacgag gattaaatta cttggacggc gcttatttta
     6361 ccaagaataa tggactggcg acggggtcgc tggttcggat caatattttg gatcaaaata
     6421 ttggcagta
//
LOCUS       DATMXK010000383         2834 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_140501, whole genome shotgun sequence.
ACCESSION   DATMXK010000383 DATMXK010000000
VERSION     DATMXK010000383.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2834)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2834)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2834
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140501"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..987
                     /locus_tag="VN363_07160"
     CDS             <1..987
                     /locus_tag="VN363_07160"
                     /EC_number="1.6.5.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01972.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_process: GO:0042773 - ATP synthesis coupled electron
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit M"
                     /protein_id="HWQ84327.1"
                     /translation="AMKLTLYLFIGSMVSLVGALAMFFAAGQGTFDMIALEQANLSPE
                     FQKLVFPFVFMGFAVLGGIFPFHNWSPDGHVAAPTAVSMFHAGVLMKLGAFAALRVGI
                     MLLPEGAKAHMWWVILLALVNVVYGAFIASIQTDFKYVIGFSSVSHMGLVTMGFATLS
                     REGLLGAGVQMFSHGVMTALFFAVVGMVYDQAHTRQIPELSGFRKVMPLVALAFIVGG
                     LVSMGMPGFSGFVAEFPIFMGVWKTTPWIAIVAAISIVITASYIIRIIGKVFFGNLPA
                     EFADHVHDVTALDKLALVFLSAILIVVGVYPSIMTPMVESGVQNILRLLGGA"
     gene            988..2463
                     /locus_tag="VN363_07165"
     CDS             988..2463
                     /locus_tag="VN363_07165"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01770.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA];
                     GO_function: GO:0016655 - oxidoreductase activity, acting
                     on NAD(P)H, quinone or similar compound as acceptor
                     [Evidence IEA];
                     GO_function: GO:0048038 - quinone binding [Evidence IEA];
                     GO_function: GO:0050136 - NADH dehydrogenase (quinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit N"
                     /protein_id="HWQ84328.1"
                     /translation="MIGGVDNLMVQAILPEILLLVLIGVVLVLDLVLSKEKDRSLGWV
                     TALGLALIIVISLVAGMPNAEGQSLWGGMVRFDWMGYVFKLVFLFAAAITALLSTDHK
                     EVGQRGEFYLLMLASTIGMCLMASAGDLVMLYLAIETTSIPMYILAGFMTRDQRSTEA
                     GFKYLLFGAFSSAVMLYGFSLLYGFAGTTSIYAIATGLQAGTVPATNIIACVLLMLVG
                     FAFKISAVPFHFWAPDVYEGAPTPVAGFLSTASKAAGFAALMRVFLIAFPGAAIEWGA
                     IATTLAVATMTLGNFLALAQKNLKRMLAYSSIAHAGYVLIGVSAIAVSAPGSDNAQFG
                     ISSVVFYLIAYLVTNLTAFGAIAVFERTTGVDDISAFNGMSRRSPGLALIMMVAMLSL
                     AGMPPLAGFIAKFFVFAAAVKVNLVWLAAVGALNSIVGLYYYMTVLKYIYLYDPAEET
                     PIGMTKPFKVALVILSVVIVLVGTVFSPIYAWSTAVAAGLF"
     gene            2527..2829
                     /locus_tag="VN363_07170"
     CDS             2527..2829
                     /locus_tag="VN363_07170"
                     /inference="COORDINATES: protein motif:HMM:NF021073.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AtpZ/AtpI family protein"
                     /protein_id="HWQ84329.1"
                     /translation="MKDKGQSNAAFPPVMLRVALQVGLFTLIIIFVSLILGLFLDKQF
                     DTEPLFLILLLVASMPLSWVGVFKIVNRAKKKLMAAASQAEAASTQIREEANRDEE"
BASE COUNT          533 a          697 c          771 g          833 t
ORIGIN      
        1 gccatgaagc tgaccctgta cctgtttata ggctcaatgg tatccctggt tggcgctctg
       61 gcgatgttct ttgctgcggg ccagggaacg tttgatatga tagccctgga acaggcgaac
      121 ttatccccag aattccagaa gctcgtcttt cccttcgttt tcatgggttt tgcggttctg
      181 ggaggtattt ttcccttcca taactggagc ccggatggtc acgttgcagc acctaccgct
      241 gtttcgatgt tccatgccgg cgttttgatg aaattgggcg ctttcgcagc cttgcgggtg
      301 ggcatcatgc ttctgcctga aggcgcaaaa gcgcatatgt ggtgggtaat tctcctggcg
      361 ctggtcaatg tggtgtacgg ggcatttatc gccagcatcc agacagattt caaatacgtc
      421 attggcttct cttccgtctc ccatatggga ctggtcacga tggggtttgc tacgttaagc
      481 cgggaaggat tgctgggggc aggcgtccag atgttctccc atggggtaat gacggctctc
      541 ttctttgctg tcgtgggtat ggtttacgat caagcccata cccgccagat accggagtta
      601 agcggctttc gcaaagttat gcccctggtc gctctggcat ttatcgtcgg cggtctggtg
      661 tcaatgggta tgccgggatt ttctggcttt gtcgccgaat tccccatctt tatgggggtc
      721 tggaaaacta caccctggat tgcgatagtg gctgcaattt cgatcgtgat taccgcgtcc
      781 tatattatcc ggattatcgg caaggtgttc tttggcaatc tgccagctga atttgccgat
      841 catgtccatg atgtgacagc cctggataag ctcgccctgg tcttcctttc agcaatcctg
      901 atcgtcgtcg gtgtctaccc gtcgataatg acacccatgg ttgagtcggg cgttcaaaat
      961 attttgcgcc tgttgggagg tgcataagtg atcggtggag ttgataactt gatggtccag
     1021 gcaatcttgc ctgaaatttt gctgctcgtc ctgattgggg ttgtcctcgt cctggatctg
     1081 gttctctcca aagaaaaaga tcgcagcctg ggttgggtta ctgctctggg cctggctttg
     1141 attatcgtca tcagcctggt ggccggaatg ccaaacgctg aaggccaatc gctttggggt
     1201 gggatggttc ggttcgactg gatgggttat gtctttaaat tagtcttctt attcgccgct
     1261 gccataactg ctttgctgtc caccgatcat aaagaagttg gccagcgcgg cgagttttat
     1321 ctgctcatgc tcgcttccac aatcggcatg tgcctgatgg cttccgcggg cgacctggtg
     1381 atgctctact tggccatcga aaccacctcg atcccgatgt atatcctggc gggctttatg
     1441 acccgcgatc agcgttccac agaagcaggc ttcaagtact tattgtttgg ggcgttttcc
     1501 tcggcggtaa tgctgtatgg ctttagtctg ttgtatggtt ttgccggcac aacctcgatc
     1561 tatgctattg ctaccggtct gcaggctggc actgtcccgg ccacgaatat tattgcctgt
     1621 gtactattga tgctggtagg ttttgcattc aagatctcag ctgtgccatt tcatttttgg
     1681 gcgccggatg tctatgaagg ggcgcctaca cctgtggccg ggttcctctc gactgcttcc
     1741 aaagctgccg ggtttgctgc tctgatgcga gttttcctga ttgctttccc aggcgcagcc
     1801 attgaatggg gtgcgatagc cactaccctg gcggtcgcca ccatgaccct gggcaacttt
     1861 ttggcgctgg ctcagaaaaa cctgaaacgg atgctggcat attcctcgat tgcgcatgcg
     1921 ggatatgttc tcatcggtgt ctccgcaatt gcggtttcag ccccaggctc agataacgcc
     1981 caattcggca tctcaagcgt agttttttac ctgattgctt acctggtgac taacctgacc
     2041 gcctttggtg cgatcgcggt ttttgagcgc acaactggtg tggatgatat ctctgcgttc
     2101 aacgggatga gccgccgctc tcctggcttg gcactgatca tgatggtcgc aatgctgtct
     2161 ctggcaggga tgccccctct ggctggcttt atcgccaagt tcttcgtctt cgccgcagct
     2221 gtgaaggtga acctggtgtg gttggcggcg gtcggagcgc tcaattcgat tgttggcctt
     2281 tattactaca tgacggtgtt aaagtatatc tacctgtacg atccggcaga agaaactccg
     2341 attgggatga ccaaaccgtt caaagtagct ctggtaatct taagtgtcgt aattgtcctg
     2401 gttgggacgg ttttctcacc tatctacgct tggagcacgg ccgtcgcggc aggattgttc
     2461 taatttgcac attgacgctc gaaatttcat tgtgttataa tccgacactg cgactggagc
     2521 aaatttgtga aggataaagg acagagtaat gcggcttttc cccctgtgat gctccgggtc
     2581 gccctgcagg tgggtctgtt tacactgatc atcatatttg tctcacttat cctggggctt
     2641 tttctggata agcagttcga tacagagccg ttatttttaa tccttttgct ggtagcaagt
     2701 atgcccttgt cctgggttgg cgtcttcaag attgtcaatc gtgccaaaaa aaagctgatg
     2761 gcagcagctt cacaagcgga ggctgcctct actcaaatcc gggaggaagc aaatcgtgac
     2821 gaagaatgat aatc
//
LOCUS       DATMXK010000384         3624 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_57561, whole genome shotgun sequence.
ACCESSION   DATMXK010000384 DATMXK010000000
VERSION     DATMXK010000384.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3624)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3624)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3624
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57561"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..981)
                     /locus_tag="VN363_07175"
     CDS             complement(<1..981)
                     /locus_tag="VN363_07175"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWQ84330.1"
                     /translation="MTETKLTRRAFLRLAALGMGGLAFRSLDRSLYLPEFPAHDRLGR
                     VVAGKVEVKARPDIDSETVGVLFDDAVLPWLSETVGPRPLWYSQRWVETPDGYIYAPN
                     LQPVRNLPNSPITALPESGGKPGMWVEVTVPYANLILMNPPARSPWLKEATLPRVYYS
                     QILWVDEIKTDAQSQIWYRVNERYGTYGDIFWVAGEALRPLAAEEMSPISPNVEAKKV
                     VIDVTYQTMACYEGSSEVFFTRISSGAKFDAQGNPVDKWSTPVGPHHIWRKLVSVHMS
                     GGTTGGGYDLPGIGWTTLFSGDGVAIHSTFWHNNFGVPMSHGCVNARPEDA"
     gene            1258..2670
                     /locus_tag="VN363_07180"
     CDS             1258..2670
                     /locus_tag="VN363_07180"
                     /EC_number="2.7.2.4"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559501.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004072 - aspartate kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate kinase"
                     /protein_id="HWQ84331.1"
                     /translation="MKFGGTSVGSPEAMAQVVAIVKQQHTIWSRLVVVTSALAGVTNR
                     LLKSASQAALGDNQTYQQNAREFLDEHMAIANHLLPDHKMRFAVKHELEQLILEFTNL
                     CHAIEILGEASPRALDAVAAQGERMAVRVLAAGLESVGVATYPVDATRIISTDDFFTD
                     AHPEMSATRLRTQRELKPLLNAGKVPVVTGFIASTPTGVTTTLGRGGSDYSAAILGVA
                     LEATEVWIFTDVDGVMSADPRLAPEAHTIEQLSYREVAELAYFGARVLHPKSIRPVIE
                     AGINLRVLNTFNPSHPGTLILADPNGGPAGMTGVQTSGKIKAVTVIRNQRLVTVEGRG
                     MLGVPGVAARTFAAVAATGTSVPLITQASSEQSICFAVPGNAIQRVLSELEASFKAEL
                     ARRDIDRIWATEEVVIITIVGAGMRNTPGISGRIFSALGGRDVNVIAIAQGSSEVSIS
                     VVVDALDTQAALNAIHSLIP"
     gene            complement(2746..>3624)
                     /locus_tag="VN363_07185"
     CDS             complement(2746..>3624)
                     /locus_tag="VN363_07185"
                     /inference="COORDINATES: protein motif:HMM:NF015817.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helicase-related protein"
                     /protein_id="HWQ84332.1"
                     /translation="TLNSFRLGQVSVLVATDVAARGLDIDDISHVFNYDLPDDPELYV
                     HRVGRTARAGKTGIAITLVARFEKRMLFNIEGFTRQKLTRATIPTEEDIRNQREQKLL
                     ASVNVWLQRGRYKRERELVESLVEQGFEPLEIAAAALKLARSEEKQRPIEHVTIFKEE
                     APERPSGRAAARYERTRQGLTRPRDSRREDTRQTESRHEDPRRSTDRSRPTSHEPGMI
                     RLALSLGKQHGVRPAEVVGLIAGQGNIPGSSIGKIFIHEDQTLVDIPEIYLAQVLKAT
                     RQAQIRRQPLELQVAS"
BASE COUNT          738 a         1050 c         1103 g          733 t
ORIGIN      
        1 agcatcctcc gggcgggcgt tgacgcagcc gtgtgacatc ggcaccccaa aattattgtg
       61 ccagaaggtt gagtggatcg ccacaccatc cccggaaaac agggtcgtcc agccaatccc
      121 gggcagatca tagccgccgc cggtggtgcc accgctcatg tgtaccgata ccagcttgcg
      181 ccagatgtga tgcggaccga ccggggtgga ccacttatct accgggttac cctgggcgtc
      241 gaatttggcg ccgctggaga tgcgggtaaa aaagacctcg ctgctgcctt cataacaggc
      301 catagtctgg taagtcacgt cgatgacaac ctttttcgct tccacattgg gactgatcgg
      361 gctcatttcc tccgcggcga gcgggcggag cgcctcgcca gccacccaga agatatcccc
      421 ataggtaccg tagcgctcgt tcacccggta ccagatctgg ctctgggcgt cagtcttgat
      481 ctcatccacc cataagatct ggctgtaata gacccgcggc agggtggctt ccttcagcca
      541 gggtgagcgg gcgggcgggt tcatcaggat caagttggcg tagggtaccg tgacctcgac
      601 ccacatcccc ggcttcccgc cactttccgg cagggctgtg atcgggctgt tggggagatt
      661 gcgcaccggc tgcaagttag gcgcataaat atagccatcc ggtgtctcta cccagcgctg
      721 ggagtaccac aatgggcgtg ggccaaccgt ctcactgagc cagggcagga cggcgtcatc
      781 gaagagcaca cccaccgtct cgctgtcgat atcggggcgc gctttcactt ctaccttgcc
      841 ggcgacaact cgcccaaggc ggtcgtgggc gggaaattcg ggtaaataca gggagcggtc
      901 cagagatcga aaagccaacc cacccattcc cagtgcagcc agccggagaa aagctcgccg
      961 ggttaatttt gtttcagtca tatccgtttg gatgccctac cagataaatt cgtcgcaacc
     1021 tatttattag cacagccagc cacttcaggc gctacaggta ctaaggatac tcgcaaacgg
     1081 ggcgcgggtc aatagccccc tcaagccggg ttcgttaaag tttagtgaga acgagccggg
     1141 aggtgattga atcgcaatta agctaaaata tgtccttgaa agagggtgtg ctaaaattgc
     1201 tcaatctcag caagaagggt gggcaggatg gcagaccaaa gtataaggac attgatcatg
     1261 aagttcgggg gcacttcagt aggcagcccg gaagcgatgg cgcaggtagt cgcgatcgtc
     1321 aaacagcagc acacgatctg gtcgcgcctg gtcgttgtca cttctgctct ggcaggagtg
     1381 accaatcgcc tgcttaaaag cgccagccag gcagccctgg gagataacca gacctaccag
     1441 cagaacgctc gggagttcct ggatgagcat atggcgatcg ctaaccatct cctccctgac
     1501 cacaaaatgc gcttcgccgt caagcacgaa ctggagcagc tgatcctgga attcaccaac
     1561 ctgtgccatg cgatcgagat cctgggcgag gcctcgcccc gcgccctgga tgcagtggca
     1621 gcgcagggcg agcgcatggc ggtgcgcgtc ctggcggcag ggctggaatc tgtcggggtt
     1681 gccacctacc cggtcgacgc gactcgcatt atcagcacag atgacttctt cacggatgcg
     1741 catcctgaaa tgagcgccac tcgcttgcgc acccagcgtg agctgaaacc gctgcttaac
     1801 gccgggaaag ttccggtggt gacgggtttt atcgcctcca cacctaccgg tgtgaccacc
     1861 accctggggc gtggaggaag tgattattca gcggccattc ttggagttgc cctcgaggcg
     1921 acagaagtct ggatttttac ggatgtggat ggggtaatga gtgctgaccc tcgcctggcg
     1981 ccggaagcgc acaccattga gcagctttcc taccgagaag tcgccgagct ggcatatttt
     2041 ggagctcgcg tgctgcaccc caagagcatc cgaccagtga tcgaagcggg aatcaattta
     2101 cgcgtcttga ataccttcaa cccgagccat ccaggcacat tgattctggc tgatccaaac
     2161 ggcgggcctg ctggcatgac tggcgtgcaa accagtggca aaatcaaagc ggtcacggtg
     2221 atccgcaacc agcgactggt gaccgtggaa gggcggggca tgctgggggt acccggcgtt
     2281 gccgcacgta cttttgctgc cgtcgccgcc acgggcacaa gtgtaccgct gatcacccag
     2341 gcttcctccg aacagtcgat ctgttttgct gtgcccggga acgccatcca gcgcgtgctc
     2401 agcgagctgg aagcatcttt taaggctgag ctggcccggc gggatatcga ccgcatctgg
     2461 gcgaccgaag aagtggtgat cattaccatt gtaggggcgg ggatgcgcaa tacccctgga
     2521 atctcagggc gcattttctc agccctcgga ggacgggatg tcaatgttat tgccattgcc
     2581 cagggatctt cggaagtctc gatcagcgtg gtggtggatg ccctggatac gcaggctgcc
     2641 ttgaacgcca ttcacagcct gataccctga ccatatttcc atcagaaact aaacggcgct
     2701 caggtgaaat ttcacttgag cgccggatga tttaatcctc aataactagg aggcgacctg
     2761 taattccagc ggctggcggc ggatctgggc ctggcgggtt gctttgagta cctgcgccag
     2821 gtagatctcg gggatatcca ccagagtctg atcctcgtga atgaagatct tgccgatgct
     2881 gctgccgggg atattaccct gcccggcaat caagcccaca acttcggcgg gccgaacgcc
     2941 atgctgcttg cccagggaaa gcgccaggcg gatcatgccc ggttcgtggg aggtcggccg
     3001 gctgcggtca gtggagcggc gaggatcctc gtggcgagat tcggtttgcc gtgtgtcctc
     3061 gcggcgtgaa tcccgggggc gggtgagacc ctggcgggtg cgctcgtagc gggcggcagc
     3121 gcgaccgcta gggcgctcag gcgcctcttc cttgaaaatg gtcacatgct cgatcgggcg
     3181 ctgcttttct tcggagcggg ccagcttgag ggcggctgcg gcaatttcca ggggttcaaa
     3241 gccctgctct accaggcttt ccaccagctc gcgctcgcgc ttgtagcggc cgcgctgcag
     3301 ccagacattg acgctcgcca gcagcttttg ctcacgctgg ttacgaatgt cttcctcggt
     3361 ggggatggtg gcccgggtca atttctggcg ggtgaagccc tcgatgttga ataacatccg
     3421 tttctcgaaa cgcgccacca gggtaatggc tatgccggtc ttcccggccc gagcggtgcg
     3481 cccgacacga tggacataca gctccgggtc atccggcagg tcatagttga agacatgcga
     3541 gatatcatcg atatccaggc cgcgggcggc aacgtcggtc gcgaccagca cgctgacctg
     3601 ccccaggcgg aagctgttca gggt
//
LOCUS       DATMXK010000385         4754 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_14335, whole genome shotgun sequence.
ACCESSION   DATMXK010000385 DATMXK010000000
VERSION     DATMXK010000385.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4754)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4754)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4754
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_14335"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1002
                     /locus_tag="VN363_07190"
     CDS             <1..1002
                     /locus_tag="VN363_07190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00350.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LCP family protein"
                     /protein_id="HWQ84333.1"
                     /translation="PRPPAPAAPGYPPAYPPADPWGETRLTPVPGEPQYPAEPPLYIP
                     PHQPPTPRHLRRRPWYVGCLGCLFKTLLVFLIPGLLLAVYLLFPGRTNLLLLGMDYSD
                     PSNSIARTDTVILSSFIPARPYVGLLSVPRDLWVPIPGYGENRINTAHFFAEAESQNS
                     GPYRAVATVEQNFGVDIDYFVRIRFEGFKDVVNALGGVDIELSEAMAGYAPGVHHLTG
                     NKALAFARSRYGSDDFFRMQQGQIVLKAILKKLTRPAGWFRVPAVYAALRRNLHTDVP
                     SWMWPRLGFAALRLGANGLDSRTVTREMVTPYTTAEGANILLPNWPLILPVVEEIFH"
     gene            1488..4511
                     /locus_tag="VN363_07195"
     CDS             1488..4511
                     /locus_tag="VN363_07195"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012311.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S8 family peptidase"
                     /protein_id="HWQ84334.1"
                     /translation="MKKLYQVLTVLVIALMAFSVPFGGQTAFAAPGEPAAPAAPAKSS
                     NNIYIVQMVDQPIVAYEGDIAGLKATAPRQGQKVNIFNKDVIAYGDYLKGRHNDALAA
                     AGGGRKVYDYTFSYNGFAAELTQDQAAKIASAAGVLAVSPDELFTIDTSSTPAFLGLT
                     DPGGLWEQLGGIGRGGEDVVIGIVDSGIWPESFSFSDRTGANGNGKKNDRLDYQQLPG
                     WNARCIPGEEFNASNCNQKLISAQYFNASWGGNPGIEAQRPWEFTSPRDYNGHGTHTA
                     STAGGNYGVPATGPASVFGTISGIAPRARISAYKALWSTQSGDTASGFTGDLVAAIDQ
                     AVADGVDVINYSISGSLTNFADPVMISYLFAARAGIFVSASAGNAGPTTATVAHPSPW
                     VTTVAAGTHNRSGEGSVTLGNGVTYFGASLANAVGPAPLINASAAGFPGADPTMVALC
                     YAADDNDGNPVLDPALVAGKIVVCDRGVTARVNKSLAVMEAGGVGVILINPAFSSLNA
                     DFHFLPTVHLQSTDFAAVHAYAETAGATATINQATVTYTTPAPLTASFSSRGPLRAGG
                     GDLLKPDVIAPGQDILAAVAPPANAGRDFNLYSGTSMSAPHVAGLAALLKDLHPEWSP
                     MMIKSAMMTSAYDVLDGPNTNPLVIFRQGAGHIRPNGAADPGLVFNSGWNDWLAFLCG
                     STSAVAPSSCSALAGLGYSFDVSDFNGASIAIGDLAGMQTVTRQVTNVGAAPATYTAS
                     VAGMVGVGVTVEPASLTLAPGETKSFTVSFTTLTATLNAYTGGQLTWTDGTHNVRLPL
                     VVRPVALGVPAAVFGTGDPITYTVSFGYNGAFSATGRGLVPAALTEGSVSDDPTDSTC
                     SLASPNAVLIPVVIPADTTYARFSLFDADVTPGTDIDLCVFRGTTLVGSSGTGTSAEE
                     VNLLNPIAEAYTVVVQGWGVNGTSPFKLHTWLLGSSDAGNMAVTAPAAAQIGVPGEIT
                     LTFSGLAPATKYLGSVAYAGAAGMPNPTIVRVDMP"
BASE COUNT          883 a         1590 c         1246 g         1035 t
ORIGIN      
        1 ccgcgcccgc ctgctccagc ggcgcctggc tacccgccgg cttatccgcc cgccgatccc
       61 tggggtgaaa ctcgcctgac ccctgttccc ggagagccgc agtaccctgc agagccgccc
      121 ctgtacattc caccccacca gcctcctact ccccgccacc tgcgccggcg accctggtac
      181 gtgggctgcc tgggctgcct gttcaagacc ctgctggtct tcttgatccc cgggttactg
      241 ctggcggtgt acctgctctt ccctgggcgc accaacctcc tgctcctggg gatggactac
      301 tccgacccat ccaattcgat cgcccgcacc gatacggtca tcctgtccag cttcatcccc
      361 gcccgtccgt atgtcgggct gctctctgtg ccgcgcgatc tgtgggtgcc gatccctggc
      421 tacggcgaaa accggatcaa caccgcccat ttcttcgctg aagccgaatc tcagaacagc
      481 ggcccttatc gcgccgttgc caccgtcgag cagaatttcg gcgtggatat cgattatttt
      541 gtgcgcatcc gcttcgaggg tttcaaggat gtggtcaatg ccctgggcgg tgtggatatc
      601 gagctgagcg aggccatggc gggttacgcg ccgggtgtcc accatctcac cggcaacaaa
      661 gccctcgctt ttgcccgcag ccgctatggt tcggacgatt ttttccgcat gcagcagggc
      721 cagattgtgc tcaaggcgat tctcaagaaa ctcacccggc cggcaggctg gttccgcgtc
      781 cccgccgtat atgccgcctt gcgccgcaat ctgcacacgg atgtcccttc ctggatgtgg
      841 ccgcggctgg gctttgctgc cctgcgcctg ggcgccaacg ggctcgactc tcgcaccgtc
      901 acccgcgaga tggtcacacc ctacaccact gccgaaggcg ccaatatcct cctgcccaac
      961 tggccattga ttctccctgt tgtggaggag attttccact gaggacgctg gctcaccaat
     1021 tctgatctgc ctacgttctg ccattccgaa acacctcccc caggtcatta ccccaaagca
     1081 gccttctgtc attgcgagag agcagggcgg tgaccctccg ccgctgcgac cgaagcaatc
     1141 taaaagtttg ctgccagatt gctgcggttc atttaaatat caaccccggc aaccggatca
     1201 gccaatccct tacttaactc gtctataatt gacatctccc catacatgtg ctatcataac
     1261 cttacatttc tgtaaggttt tcattgtcta ttgcgacgaa gcgaaatggc tccttatctt
     1321 atccagatcg ataagtgaga tcgtcgccat gttattaaac atttgtccga ttctttcaca
     1381 attgaatagg aggtgatgcc cccccgccag acaatggtcc tatccaagca cctgttgcag
     1441 ttcgaactat acaaggtcta actttcgttt tgggaggaat caggaaaatg aagaagctgt
     1501 accaagtatt aactgttctg gttatcgcct taatggcatt ctctgtgcct ttcggcgggc
     1561 aaactgcttt tgccgctccc ggtgaaccag ctgcgccggc tgcccctgcg aaatcaagca
     1621 acaatatcta tatcgtgcaa atggtcgacc aacccattgt cgcctatgag ggtgatattg
     1681 ccggtttaaa agcgaccgca ccgagacagg gccagaaggt caacatcttc aacaaagatg
     1741 tgattgccta tggtgattat ctcaagggac gccataatga cgccctggcc gcggccggcg
     1801 gtggacggaa agtgtatgat tacaccttca gctacaatgg ctttgcggca gagctcactc
     1861 aggatcaggc tgccaaaata gcctctgcag caggcgtgct ggcggtttca ccggatgagc
     1921 tgttcactat cgatacctca tccacgccag cgttcctcgg cctcaccgat ccgggtgggc
     1981 tgtgggaaca gctgggcggg atcggtcgcg gcggtgagga tgtcgtgatc ggtatcgtcg
     2041 actccggcat ctggcccgaa agcttcagct tctcggaccg caccggcgca aatggcaatg
     2101 gcaagaagaa tgatcgcctg gattaccagc agctccctgg ctggaatgcg cgctgcatcc
     2161 ccggcgagga attcaatgcc tcgaactgca atcagaagct gatcagcgcc cagtatttca
     2221 atgcatcctg gggcggcaat cctggcattg aagcccagcg cccgtgggaa ttcacttccc
     2281 cccgtgatta caacggtcac ggcacgcaca ccgcctccac cgccggtggc aattacggtg
     2341 tcccggccac gggtccggct tccgtttttg gcaccatcag cgggatcgcc ccgcgcgccc
     2401 ggatctctgc ttataaggcg ctctggtcaa cccaatctgg cgacaccgcc agcggcttca
     2461 ccggcgacct ggtggcagcc atcgaccagg ccgttgctga cggtgtggat gtgatcaact
     2521 actcaatctc cggctcgctg accaacttcg ccgacccggt gatgatttct tacctgtttg
     2581 ctgcccgcgc cggcatcttt gtatccgcct cggccggcaa cgctggtcca acaactgcca
     2641 cagtggccca ccccagcccc tgggtcacca ccgtcgcagc cggtacccat aaccgctccg
     2701 gggaaggctc cgttaccctc ggcaatggcg tgacctactt tggcgcttca ctcgccaacg
     2761 cagtcggccc agccccgctc atcaatgcat ctgccgccgg tttcccaggc gcagatccga
     2821 ccatggtagc cctgtgctac gctgccgatg ataatgacgg taatccggtg ctggatccag
     2881 cgctcgtcgc cggtaagatc gtggtttgcg atcgcggcgt aaccgcccgc gtaaataaga
     2941 gcctggcagt catggaggct ggtggggtcg gtgtgatctt gatcaacccg gcatttagct
     3001 ccttgaatgc tgacttccac ttcctcccca ccgtgcattt gcagagcaca gatttcgcag
     3061 ccgtccacgc gtatgctgaa acggctggcg cgactgccac aatcaaccag gcaaccgtca
     3121 cctacaccac cccggctccg ctgaccgcct cattctcctc gcgcggcccg ctgcgcgccg
     3181 gtggcggtga cctgctcaag ccggatgtga tcgcacccgg acaggatatc ctggctgcgg
     3241 tcgcaccacc tgccaatgcc gggcgggatt tcaacctgta cagcggcacc tctatgtctg
     3301 ctccgcacgt cgccggcctg gctgccctgc tcaaggacct gcaccccgaa tggtccccga
     3361 tgatgatcaa atcggcgatg atgaccagcg cctatgacgt cctcgatggc ccgaatacca
     3421 acccgctggt gatcttccgc cagggcgccg ggcacatccg ccccaacggt gcagccgatc
     3481 ccggcctcgt gttcaactca ggctggaacg actggctggc gttcctgtgc ggatctacct
     3541 ctgctgttgc tccttccagc tgcagcgctc tggctggatt gggctactcc ttcgatgtca
     3601 gcgacttcaa cggcgcctcc atcgccatcg gcgacctggc aggcatgcag acggtcaccc
     3661 gccaggtgac caatgttggc gcagctccag cgacctacac tgcctcggta gccggcatgg
     3721 tgggggttgg tgtgactgta gagccggcct cgctgaccct ggctcctgga gagactaaat
     3781 ccttcaccgt cagcttcacg accctcactg ccacactcaa tgcttacacc ggtgggcagc
     3841 tcacctggac cgacggaacc cataatgtcc gcctccccct ggtcgttcgc ccggtagctc
     3901 tgggtgtccc ggctgccgtg tttggcactg gagacccgat cacctatacc gtgagcttcg
     3961 gctataatgg cgccttctcg gcgacgggtc gcggcctggt cccggcagcc ctcaccgaag
     4021 gttcggtctc agacgatccg accgacagca cctgctcgct ggcttcaccc aacgctgtcc
     4081 tcatcccggt cgtgatccct gcggatacga cctacgcccg cttctccctg ttcgatgccg
     4141 acgtaactcc aggcactgac atcgacctgt gcgtgttccg cggcacaact ctggttggca
     4201 gcagcggcac gggcacctcg gctgaagagg tcaatctgct caacccgatc gctgaagcct
     4261 acaccgtggt tgttcagggt tggggtgtga acggcacatc gcccttcaag ctccatacct
     4321 ggctgctcgg cagctccgat gctggcaata tggctgtcac cgctccggcc gccgcccaaa
     4381 ttggcgtccc cggcgaaatc accctgacct tcagcggtct ggccccggcg accaaatacc
     4441 tcggctccgt tgcttacgcg ggtgcagctg gcatgcccaa ccccaccatc gtgcgcgtcg
     4501 atatgcctta atcctctctg aaaaccggat tttttctgag ctcgcacttt gagctcaaat
     4561 ccggttctcc cgatcacgac agaaaccggg cttcacagcg aagcccggtt ttcatttcac
     4621 actcgaaaac cgagtttttt ctgagctcgc actttgagct caaactcgtt ttctattcat
     4681 cctcccagaa gctcgccctg cctctttatg ataaaatccc tcccatgaaa gatgaattca
     4741 aagtcgttgc cacc
//
LOCUS       DATMXK010000386         6936 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_52728, whole genome shotgun sequence.
ACCESSION   DATMXK010000386 DATMXK010000000
VERSION     DATMXK010000386.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6936)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6936)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6936
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52728"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..363
                     /locus_tag="VN363_07200"
     CDS             <1..363
                     /locus_tag="VN363_07200"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWQ84335.1"
                     /translation="WQEIKRHPVNGADMLRNIPYLAPAIPIVRYHHERWNGQGYPDGL
                     IGEEIPQGARIVAVADSFDAMTTNRPYSPARSLENAMEEIQRCSGTSYDPAVVEVFQS
                     FWAEGQIKQISLLWKEKH"
     gene            complement(365..3100)
                     /locus_tag="VN363_07205"
     CDS             complement(365..3100)
                     /locus_tag="VN363_07205"
                     /inference="COORDINATES: protein motif:HMM:NF013645.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84336.1"
                     /translation="MSKFEPERILNSRRALLKDLPRITRAEEAAEGGCGVLGFAANQP
                     VAGRHVLAASRQMHNRGNGKGGGIAMVGLDPQQARVDASTLESHYMLQVALLDPACRP
                     ELEAHFIQPKFEIAQAYPLEHVDDFHQIAGLEVQPPDVWRYFVRVKPEQLEQFGRAQG
                     LLGQPGKTDLEPRALEDEFVYQNSFHLNQQFYASLGEKRAFVVSHGRNLSVLKIVGYA
                     EDVVEYYRLEDQTAYVWIAHQRYPTKGRVWHPGGAHPFIGLNEALVHNGDFANYHAVS
                     EYLRQRSIGQLFLTDTEVSVQLFDLWDRVYGYPLEITLEAMAPTSEHDFQMLPPERQS
                     LYRAVQRTHIHGSPDGPWFFIVARSKDKVQTLESQAGADSLSYELLGITDTSMLRPQV
                     FAMYENQPDGEGEAAGPVQIGLVASERQAINACLRSLAAEDERYQPVADRYWVARGGS
                     HTDGGAFRFVVDAQESDSPGPARSQHRLHCTNKFGTPVAVDAQRRHSDRTALDPERTA
                     PALRMDWENASRHAFDDGGATTFWQWFEARLPAMNWDELAWAMEEMVDFAAQGPEEWE
                     YARQSLSLLRDRRVDLGDKKRASLLALVDRYLARLFELAPQFETPGDGFSRRLTWDTR
                     SALVEPDVRDGVTPPVLVIDTAGFPPEGEEAAARLIVRAYELGWRHIAAYNWTGGRFA
                     ACGLGPDSFDLRLDLYGDVGDYAASGLDGAEVHLHGDGQDQLGQIMKRGKLVIHGDTG
                     QTFLYGAKGGEVYVLGSAAGRPLINAVGRPRAVINGTCLDYLAESFMAGDPHQGGGFV
                     ILNGVTYAESGTLVELETPYPGGNLFSLASGGAIFLRDPHKRVAEDQLNGGAFGELSV
                     SDWELIKPYLQENERLFGIRMADLLKVDGKTCQPAEVYRKVEVRDASILRETSNR"
     gene            complement(3135..4073)
                     /locus_tag="VN363_07210"
     CDS             complement(3135..4073)
                     /locus_tag="VN363_07210"
                     /inference="COORDINATES: protein motif:HMM:NF013088.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="EamA family transporter"
                     /protein_id="HWQ84337.1"
                     /translation="MQKPSNLAIWAGLITIYIVWGSTYLAIRFAIETIPPFLMAGSRF
                     MIAGLILFTWRRLAGDPLPGRKEWLSAAVVGLLLLMIGNGGVTWAELTVNSGIVALLV
                     GTVPLWVVLVEALRPGGKWPGWQGMLGVLLGVVGIVILVNPWNSSSGLHLSGVLVVIV
                     AAAAWAVGSVYSHNAPLPKSPLMGTSLEMLVGGVALLLLGSITGEWSQVSWAAISGKS
                     ILGLAYLIVFGSLIGFASYSWLLRVAPTSLVSTYAYVNPLIAILLGNLLAEEPLTPQV
                     AIAAVVIVSAVVLINSARAPKVKIEPAYALVERECD"
     gene            complement(4105..6585)
                     /locus_tag="VN363_07215"
     CDS             complement(4105..6585)
                     /locus_tag="VN363_07215"
                     /inference="COORDINATES: protein motif:HMM:NF013784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015930 - glutamate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0016638 - oxidoreductase activity, acting
                     on the CH-NH2 group of donors [Evidence IEA];
                     GO_process: GO:0006537 - glutamate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamate synthase-related protein"
                     /protein_id="HWQ84338.1"
                     /translation="MSSHSRYHISTHPAPAPLAGYPAQFHLDDDGAYVKYGHQVFMGS
                     TQRGDPYWTAELITQTWFQAETGRIPISGAGYGGKFAGEGFEGLWLDMSEIVRPTRDG
                     IHGREVISTQVDLGGNLLSLDFDAQGALSSRLPAVVSLPLPVLLGPLVMPLPGRGALQ
                     AMAKAAASLETLALIDPADYAEDLAPWVDWLAPRFMNSLADLPAGWQPGYIELEADVL
                     ENIPVLWAEAQERFPSAVIAARLALSEAAPRQAAAWVAAGIPVIHLFADDVGQDLDGQ
                     HLPDAIRAVHMHLVGLAVRDAVTLIVSGGIGAAEHVAKAVACGVDLAAVDFVLQVAWG
                     CSLWADKGNCSVENEELDPEWGAQRVINLMNAWRDQLLEALGAMGMREIRRLRGEVGR
                     TIWHTTEESTFLSILGGSEPRSRYSYLPRPAGAEGDFRWPTPLLLSSQQQARSGTLPT
                     GDLEYRVGASDGGFDRLAFAFELDRDPREIIAADPAGFDLSLLLNRRDPNRPERRLPI
                     PWYGGGMSYGSISLPVMLARARAAQRLGSYISTGEGGYPEALEPYAANMITQVATGLF
                     GVSEASIQRAPVVEIKYAQGAKPGLGGHLLGNKNTSTVAALREAVEGTSLFSPFPFHS
                     VYSVEDHKKHVDWLKQVNPQALISVKVSTPTDVDMVAVGSYYAGAHIVHLDGAYGGTG
                     AAPEIAKKNIAMPIEYALPKVHRFLEGEGIRQEITLMASGGLRSAWDVAKAIALGADG
                     AVLGTAELVALGCIRIAQCEKGLGCPFGITTTDPEQARLIDADLGFQQIVNLYTSWLW
                     QLSGILEILGMHSISELRGRSDLLVYLN"
BASE COUNT         1567 a         2167 c         1854 g         1348 t
ORIGIN      
        1 tggcaggaaa ttaagcgcca tccggttaac ggagctgata tgctcaggaa tattccgtac
       61 ctcgctcccg ccattccgat cgtgcgttat catcacgaac gctggaacgg acagggctat
      121 cccgatggtc tgattggaga agaaataccc cagggggcgc gcattgtggc agtggcggat
      181 tcattcgatg cgatgaccac gaaccggcct tatagcccgg cgcgcagtct ggaaaatgca
      241 atggaagaaa tccagcgctg ctcaggcacc tcgtatgacc cggcggttgt ggaggtattc
      301 cagagctttt gggcagaagg ccagatcaag cagatttcac tgctgtggaa agaaaagcat
      361 tagctcagcg attgctggtt tcgcgcaaga tactggcgtc gcgcacctct accttgcgat
      421 acacctcagc cggctggcat gttttcccat cgaccttgag cagatctgcc atgcgaatgc
      481 caaataagcg ctcattttcc tgaagataag gcttgatcag ctcccaatct gacaccgaaa
      541 gctctccaaa cgccccccca ttgagctgat cttccgccac gcgcttgtgg ggatcgcgca
      601 ggaagatcgc accgccagaa gccagcgaga acaggtttcc tccgggatag ggcgtctcca
      661 gctcgaccag ggtgccgctt tcagcatagg tgacgccgtt caagatcaca aacccaccgc
      721 cctgatgtgg atcaccagcc ataaaagatt ctgccaggta atccaggcag gtgccattaa
      781 tcaccgctcg cgggcgtccc acagcattga tcagcgggcg gccagctgca gagcctaata
      841 catacacttc accgcctttg gcgccataca aaaaggtttg accggtgtcc ccatggatta
      901 ccagcttgcc ccgcttcata atttgaccca gctggtcctg accatcccca tgcaggtgca
      961 cctcggcccc atccaggcca gaggcagcgt aatcacccac atcgccatac agatcaaggc
     1021 gtaaatcaaa gctatccgga cccaggccgc aggctgcaaa acgcccgccg gtccagttat
     1081 aggcggcgat gtgcctccag ccaagctcat aggcgcgcac aattaaacgg gcggcagctt
     1141 cctcgccttc cgggggaaag ccagctgtgt caatcacgag taccggtggg gtaacaccat
     1201 cgcgaacgtc cggctctacc agcgctgagc gagtatccca ggtcaggcgg cgggagaacc
     1261 catcgccagg tgtctcaaac tggggggcta attcaaacag ccgggccaga tatctgtcaa
     1321 ccagagccag cagggaggct cgttttttat cccccagatc cacccgccgg tcgcgcagca
     1381 gggataaaga ctggcgagca tactcccatt cttccggacc ctgagctgca aaatccacca
     1441 tctcctccat tgcccaggct agctcatccc aattcatcgc cggtaagcgc gcctcaaacc
     1501 actgccagaa tgtcgttgct ccgccatcat caaatgcatg ccggctggca ttttcccagt
     1561 ccatgcgtaa ggcgggcgcg gtccgctctg gatccagagc ggtgcgatcg ctgtgccggc
     1621 gttgggcgtc gacagcaacc ggtgtcccaa atttgttggt gcagtgcagg cggtgctggc
     1681 tgcgggcagg accaggggag tcactctcct gagcatctac cacaaaccgg aatgcgccgc
     1741 catccgtatg cgatcccccg cgtgccaccc aatagcgatc agctactggc tggtagcgct
     1801 catcttcagc agctaaagag cgcaagcagg cattgatcgc ctggcgttct gaagctacca
     1861 gaccaatctg caccggtccg gcagcctcgc cttccccatc cggttgattc tcgtacatcg
     1921 caaaaacctg cgggcggagc atactggtat ctgtgatccc caataactcg taactcaggc
     1981 tgtctgcacc ggcctggctt tccagagtct gcaccttgtc cttggagcgg gccacaataa
     2041 agaaccaggg accatccggc gagccatgta tgtgagtgcg ctggacggcg cggtacaggg
     2101 attggcgctc aggtgggagc atctggaaat catgttcgct ggtcggcgcc atcgcttcca
     2161 gggtgatctc caacgggtag ccgtaaacgc ggtcccagag atcgaataac tggaccgata
     2221 cctctgtatc cgtcaaaaaa agttgcccga tactgcgctg gcgcaggtat tcgctgaccg
     2281 cgtggtaatt ggcgaaatcc ccattgtgaa ccagggcttc gttcaggccg atgaatgggt
     2341 gcgccccacc cggatgccag acgcgcccct tggtcgggta gcgttggtgg gcgatccata
     2401 cataggcggt ctggtcctca agacgatagt attccacgac atcctcggca taacccacaa
     2461 tcttcaatac cgaaagatta cgcccgtggc tgaccacaaa ggcgcgcttc tcccccaggc
     2521 tggcatagaa ctgctggttc aggtgaaacg agttctggta gacgaattca tcttccaggg
     2581 cgcgcggttc cagatcggtc ttgcctggct ggcccaggag accctgcgca cgaccgaact
     2641 gctccagctg ctctggtttc accctgacaa aatagcgcca aacgtccgga ggctgaacct
     2701 ccaatccagc gatctggtgg aaatcatcca catgctccag cggatatgcc tgggcaatct
     2761 caaatttggg ctggatgaaa tgagcctcca attccgggcg gcatgcaggg tccagcagtg
     2821 ccacctggag catataatgg ctctccaggg tgcttgcgtc cacccgcgct tgctgcggat
     2881 cgagacccac catcgcgatt cccccacctt tgccattgcc ccggttgtgc atctggcgag
     2941 aagctgccag cacatgcctc cccgcgacgg gctgattggc tgcaaatccc agcacgccgc
     3001 agccgccttc ggcagcttct tcggcgcggg ttatccgggg cagatccttc aacaaagccc
     3061 ggcgtgaatt caatatccgt tctggttcaa atttactcat agattctcca acatgctcaa
     3121 caaccacccc tgcgtcaatc acattctcgt tccaccagcg cataagccgg ttcaattttt
     3181 actttgggag cccgggcaga atttatcaat acaactgcgc tgacgatcac cactgctgca
     3241 atcgcaacct gaggcgtcaa tggctcttct gccagcaagt tacccagaag gatcgcaatc
     3301 agcgggttca catacgcata ggtcgagacc agagatgtgg gggccaccct caagagccag
     3361 ctgtaagaag cgaaaccaat tagcgagccg aaaacgatca gataagccag tcccagaatg
     3421 gattttccac taattgccgc ccacgatact tgagaccact ccccggtgat tgatcccaaa
     3481 agcagcagcg caacaccgcc gaccagcatt tccaggctgg tgcccattaa gggtgatttc
     3541 ggcagtggcg cgttgtggct gtagacagag ccaacagccc aggctgcggc tgctacaatc
     3601 accaccagta ccccggagag gtgtaatcct gagctggaat tccagggatt gaccaggatc
     3661 acgataccca cgacgcccag caagaccccc agcatgcctt gccacccggg ccattttcca
     3721 cccggacgta aagcttctac cagcacaacc caaagcggta cggtgccaac cagtaaggcg
     3781 acaattccag aattgacggt cagctctgcc caggttacgc cgccattacc gatcataagc
     3841 agcagcaagc ccacaaccgc ggctgaaagc cattccttgc ggcctggaag gggatcacca
     3901 gcaagtcgcc gccaggtaaa gaggatcagc ccggcgatca tgaaacgtga tcccgccatc
     3961 aggaagggtg ggatggtttc gatggcaaaa cggatcgcga ggtaggtaga tccccatacg
     4021 atatagatgg tgatcagacc ggcccatatg gcgagattgg atggtttttg cattcttatt
     4081 cctgccttac tcgattcaaa ccttctaatt caggtacacc aacaaatcac ttcgcccgcg
     4141 taattcggag atcgaatgca tccccagaat ttcgaggatt ccagagagct gccacagcca
     4201 ggaagtatac agattaacga tctgctggaa acccaggtca gcatcaatca gcctggcctg
     4261 ctctggatcg gtcgtcgtga tcccaaacgg gcagcccaat cctttttcac attgggcgat
     4321 gcgaatgcag cccagcgcca ccagctcggc tgtgccgagg actgccccat ccgctccaag
     4381 ggcgatcgcc ttcgccacat cccaggctga gcgcaaaccg ccagacgcca tcagggtgat
     4441 ttcctggcgg attccttcgc cttccaaaaa tcggtgcacc tttggcaggg cgtactcgat
     4501 cggcatggcg atatttttct tggcgatctc tggagctgct cccgttcccc cataagcacc
     4561 gtccaggtgg acaatgtgcg ccccggcata atacgagccg accgccacca tatccacatc
     4621 cgtcggcgtg gaaactttga cgctgatcaa cgcctggggg ttcacctgct taagccagtc
     4681 gacgtgcttt ttgtggtctt ccacggaata aacactgtgg aaggggaagg gtgagaataa
     4741 ggatgtccct tccacagcct cccgcagtgc ggcgacagtg gaggtatttt tgttgcccaa
     4801 caggtggcct cccaggccgg gcttggcgcc ctgggcatat ttaatctcaa ccaccggggc
     4861 gcgctggatg ctggcctcgg aaaccccaaa aagaccggtc gctacctggg taatcatatt
     4921 cgccgcatag ggctcaagcg cttccgggta gccgccttcc ccggtcgaga tataggatcc
     4981 caggcgctgg gcagccctgg cacgtgccag cataaccggc aaactgatgc tgccatacga
     5041 cattcctccc ccataccagg gaatgggtaa acgccgctcc gggcggttag gatcgcgccg
     5101 gttgagcagc aggctgaggt caaagcctgc cggatcagcg gcgataatct ctcgcggatc
     5161 acgatccaat tcgaatgcaa aagccagccg gtcaaaaccg ccatcggatg cgccaacgcg
     5221 atattccaga tcgccggtgg gcagtgtccc gctgcgtgcc tgctgctgtg agctgagcag
     5281 gagcggagtc ggccagcgaa aatcgccctc ggcgccggct ggtcggggta gatagctgta
     5341 gcggctgcgc ggttcactcc cgcctaagat cgatagaaaa gtactctcct cggtagtgtg
     5401 ccagatcgtc cggcctactt cgccgcgcag gcggcggatt tcccgcatac ccatggctcc
     5461 caacgcttcc aggagctgat cccgccaggc gttcatcagg tttatcaccc gctgcgcacc
     5521 ccactccggg tccaattctt cattttctac actacagttg cctttatccg cccataaaga
     5581 gcagccccag gcgacctgca ggacaaaatc aacggctgcc agatccacac cgcaagccac
     5641 cgccttggcg acatgctccg cggcaccaat cccaccgcta acgatcaggg tcactgcatc
     5701 gcgcaccgcc aaacctacca ggtgcatatg tacagcccgg attgcatccg gaagatgttg
     5761 accgtccagg tcctgcccga catcgtcggc gaaaaggtga atcaccggga tgccggctgc
     5821 tacccaggcc gcggcttgcc ggggggctgc ctcagacagc gccaggcggg ctgcaatcac
     5881 ggcggaagga aaacgctcct gcgcctctgc ccacagaaca gggatatttt caagcacatc
     5941 cgcttcaagc tcgatataac caggctgcca cccggcaggc agatctgcca ggctgttcat
     6001 gaaacgcggc gctaaccagt ctacccaggg agccaggtcc tcggcataat ctgcaggatc
     6061 gatcaacgcc agggtctcca gactcgctgc cgccttcgcc atggcctgca gggcaccccg
     6121 gcccgggagc ggcatcacca gcggacccaa taatacaggc aaaggcaggc tcaccacagc
     6181 aggcagccgg gaggacagcg ccccttgagc atcaaaatcc agagacagta aattaccgcc
     6241 caggtcaacc tgggtagaga tcacttcccg cccgtgaata ccatcccggg tggggcgcac
     6301 aatctcactc atatccagcc acagcccttc aaaaccttcc ccggcaaatt ttccaccgta
     6361 gcctgccccg gatatcggga tccgcccggt ttcagcctga aaccaggtct gggtgatcag
     6421 ctcggcggtc caatagggat cgccgcgctg ggtgcttccc ataaagacct ggtgcccgta
     6481 tttcacataa gcgccatcat catccagatg aaattgagct ggatatcctg ccaatggagc
     6541 aggggccggg tgagtgctga tatggtagcg actgtgcgag ctcatatata aaaaacctcc
     6601 gccatccgct taaacaataa aacaggcacg ctgggcgtgc ccattcttaa aatcaattaa
     6661 caggaaacaa tgggtgcgcc cctccgaata tgaccttgtc cacagctaaa agccgggaca
     6721 gccactgcgc tgcgctttgt cggcgggttg cccacgccgg tttcacgtca cgctcatcgg
     6781 gaggggtata cgcgggatat ggcgatcccg ggaggcatcc gctgatctct cgattttctc
     6841 ccagcgcaag tagcgtaacc ttcctgctgc tggaattaac cctgccgcgc ggcagggaac
     6901 ctccacctcg tcaccctggt cgaacatttc cagggc
//
LOCUS       DATMXK010000387         4558 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_153108, whole genome shotgun sequence.
ACCESSION   DATMXK010000387 DATMXK010000000
VERSION     DATMXK010000387.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4558)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4558)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4558
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153108"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..280
                     /locus_tag="VN363_07220"
     CDS             <1..280
                     /locus_tag="VN363_07220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84339.1"
                     /translation="PEIVRNLFFQLKIKLFADQAGLASITSENSQLVLRFTEGELPEL
                     LPELGPAVRQGKTALWIPMNTLPDWPARLYEVLERLAAHRSAYQTQPG"
     gene            283..924
                     /locus_tag="VN363_07225"
     CDS             283..924
                     /locus_tag="VN363_07225"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558539.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWQ84340.1"
                     /translation="MAIFPRGVPLSKRLLDLLLAGVGMVILSPLFLFLIILVAWRLGT
                     PVFFRQRRPGYRAIPFEIFKFRSMSDARDDQGKLLPDEQRLTGLGRFLRAASLDELPE
                     LFNVLRGEMSLVGPRPLLLQYLERYTPEQARRHDVLPGITGWAQINGRNVITWEDKFR
                     LDIWYVDHQSLTLDIKILLLTVWKVIRREGISQPGHATAEEFMGTPPEQRTSL"
     gene            921..1841
                     /locus_tag="VN363_07230"
     CDS             921..1841
                     /locus_tag="VN363_07230"
                     /inference="COORDINATES: protein motif:HMM:NF012880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine pyrophosphate-dependent dehydrogenase
                     E1 component subunit alpha"
                     /protein_id="HWQ84341.1"
                     /translation="MTNPIPDPPALYRALCRIRAFEETVLENFPRGVFFGTTHTYLGQ
                     EANAVGVLSHCLPQDIVFSNHRCHGHFLAYGGDPRALFAELMGKASGVCGGRGGSQHL
                     QWKNFYSNGIQGGIVPVATGMALAEKLQGRPAVVFTFLGDGTMGEGVLYEALNLANLW
                     HAPVLFVLENNHIAQTTPIEIAVAGSLAARFTAFGIPVQELDSSDILEIYPVAGNLVD
                     EIRQTQTPRALILNTCRFGPHSKGDDTRSLEALEQMKTQRDPLLIQAARLESAQINLI
                     HFKTGAEIAEAFQNALTDPFPIQPDPGISI"
     gene            1838..2872
                     /locus_tag="VN363_07235"
     CDS             1838..2872
                     /locus_tag="VN363_07235"
                     /inference="COORDINATES: protein motif:HMM:NF014798.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transketolase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ84342.1"
                     /translation="MTTVLDSLNHALHAAMQAHPGVYLLGEDLLDPYGGAFKVARGLS
                     TAFPNRVLSSPISEAGLVGLASGMALRGLRPVVEIMFGDFLTLAADQLINHAAKFRWM
                     YNDQVRVPLVVRTPMGGRRGYGPTHSQSLEKHFLGTPGLRVLAPCDLGNPGMLLQQAI
                     LEDDDPVLFIENKLLYLCRIPKTADLADFELRTLLLESASAPGLDQQAYAPAYELNIR
                     SAPPARLTIAAYGYMAHLARQAQYKLAYEHEIFAELIILTQLAPFTPGPVLDSVQRTG
                     RLLTVEEGTLPLGWSAEIIAETAAALGSRLLASRRLAALNSPIPASGPLESETLPDIT
                     TIIRAAQSMV"
     gene            2897..3529
                     /locus_tag="VN363_07240"
     CDS             2897..3529
                     /locus_tag="VN363_07240"
                     /inference="COORDINATES: protein motif:HMM:NF012584.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="biotin/lipoyl-containing protein"
                     /protein_id="HWQ84343.1"
                     /translation="MNSPIPVTTPLLNPNEPEASLAALHVQNGQHVTTGDPLFTLETT
                     KSAADVQAEEAGYVTGIQVHIGDTVQAGEILCYLADSPEWTPAEPAVQLTPQTDQPVP
                     EGLRITHPALELARQSLVDLSQLPRDQLITETTLRAYLTPHSGEKTEYSLPPTSVDST
                     AVIIYGGGGHAKALIDVIRAMGVYRIAGVLDDNPSVQGEILGVPVLGGAE"
     gene            3674..4003
                     /locus_tag="VN363_07245"
     CDS             3674..4003
                     /locus_tag="VN363_07245"
                     /inference="COORDINATES: protein motif:HMM:NF012360.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84344.1"
                     /translation="MEASANLSAGGQVFPLAYVGSEVKAGFGSIINTGAIISHECIIG
                     DFVNISPGAILAGQVQIGNGALVGMGATVNLQVTIGAGARIGNGSTVKSDVPAGGVVR
                     AGGIWPA"
     gene            4071..>4558
                     /locus_tag="VN363_07250"
     CDS             4071..>4558
                     /locus_tag="VN363_07250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559271.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DegT/DnrJ/EryC1/StrS family aminotransferase"
                     /protein_id="HWQ84345.1"
                     /translation="MSSPDLSDAERQAVTAVLNTPVLSMGKEIQAFEAALQELTGWQH
                     AIAVNSGTSGLHLCIRAAGVGINDGQTGDMVLTTPFSFVASTNVILYEQATPIFVDVD
                     PITGNIDPALAVQAIKELASGSQAARRWLPRKGWKEGGQLKAILPVDVFGQPADMDPL
                     VQT"
BASE COUNT          966 a         1356 c         1235 g         1001 t
ORIGIN      
        1 tccagaaatc gtccggaact tgtttttcca acttaaaatc aagctgtttg cagaccaggc
       61 cgggctggca tcgattacat cagaaaacag ccagctggtg ctgcgcttca ctgaaggtga
      121 gttgccggag ctcttacccg agctgggacc ggctgtccgg cagggaaaaa ctgccctgtg
      181 gattcccatg aacaccctgc ccgattggcc ggcgcgcctc tatgaggtcc tcgaacgtct
      241 ggctgcccac cgcagtgctt accagaccca acccggatga atatggcgat attcccaagg
      301 ggtgtcccac tctccaaacg tttgctcgac ctgctcctgg ctggggtggg tatggtcatt
      361 ctttcacccc tttttttgtt cctgataatc ctggttgcat ggcgattggg aacccccgtt
      421 tttttccgcc agcgccggcc aggataccgt gccatcccct ttgagatctt caaatttcgc
      481 agcatgagtg atgcccgaga tgatcagggt aagctcctcc ccgatgaaca gcgtctcact
      541 ggcttagggc gtttcttgcg cgccgccagt ctggacgaat tacccgagct tttcaacgtg
      601 ctgcggggtg aaatgagcct ggtaggcccc cgccccctgc tcttgcagta cctggagcgc
      661 tacaccccgg aacaggcccg gcgtcacgat gtcctcccgg ggattaccgg gtgggctcag
      721 attaatggac ggaatgtcat cacctgggag gacaaatttc gcctggatat ctggtatgtc
      781 gatcaccaga gtctgacgct cgatatcaaa atccttctcc tgacggtttg gaaagtcatt
      841 cgccgggagg ggatcagcca gcccgggcac gccactgccg aggaattcat gggtacccca
      901 ccggagcaac gcacttccct atgaccaatc caatcccaga cccaccagca ctgtacaggg
      961 cattatgccg tatccgcgcc ttcgaagaaa ccgtcctgga aaacttcccc aggggagtgt
     1021 tcttcggcac cacccacaca tacctgggtc aggaagcgaa cgccgtcggt gtgctcagtc
     1081 actgcctgcc acaggatatc gttttcagca atcaccgctg ccacggtcat tttctggctt
     1141 atggcggcga tccccgcgcc ttgttcgccg aattaatggg caaagccagc ggagtatgcg
     1201 gcgggcgggg cggatcgcag cacctgcagt ggaaaaattt ctactcgaat ggcattcaag
     1261 gtgggatcgt tccagtcgcg acaggtatgg cgctggctga aaaactccag gggcgccctg
     1321 cagtcgtttt tacattcctg ggggatggca caatggggga aggtgtgctg tatgaagcgc
     1381 taaatctggc caatttatgg catgcaccgg tcctgtttgt gctggagaat aaccacatag
     1441 ctcaaaccac cccgatcgag atcgcggtgg caggcagcct ggctgcccgt ttcactgcgt
     1501 ttggcattcc ggttcaggag ctcgacagca gcgatatcct tgaaatctat ccagtcgccg
     1561 gaaatctggt tgacgaaatc cgccagactc agacccctcg cgctttgatc ctgaacacct
     1621 gccgctttgg gccgcattcc aaaggcgatg ataccaggtc tttagaagca cttgagcaga
     1681 tgaaaaccca acgcgatcca ctcctcattc aagctgctcg gctggaaagc gcacaaatca
     1741 atctaatcca tttcaaaacc ggcgctgaga tcgctgaggc tttccaaaat gccctgacag
     1801 atcccttccc tatccagcca gatccaggca tttccatatg acaactgtcc tggactcact
     1861 caatcacgcc ttacatgctg cgatgcaagc ccatcccggt gtttatctgc tgggggagga
     1921 tctcctcgat ccctatggcg gtgcgttcaa ggttgctcgc gggctttcaa cggcttttcc
     1981 caaccgggtt ttatccagtc caatatccga agccgggctg gtgggtctgg caagcgggat
     2041 ggctttgcgc ggcctgcgcc cggtcgtgga aatcatgttt ggcgacttcc tgactctggc
     2101 agccgaccag cttataaatc acgccgcaaa atttcgctgg atgtacaacg accaggtgcg
     2161 agtgcctttg gtcgtccgga ctccgatggg cgggcggcgt ggctatggtc cgactcacag
     2221 ccagagcctg gaaaagcatt tcctgggaac accaggcttg cgtgtccttg ctccatgcga
     2281 ccttggaaat ccggggatgc tcctccagca ggcgatcctt gaagacgatg atccagtgct
     2341 gttcattgag aacaagctgc tctatctctg ccgcattccc aaaacagccg atctggctga
     2401 ttttgagctg cggaccctgc tccttgaatc cgcctcagct cctggcctgg atcaacaggc
     2461 ttatgcgcct gcctatgaat taaacattcg cagtgctcca cctgcccggc tgacgatcgc
     2521 cgcctacgga tatatggcgc acctggcaag gcaagcccag tataagctgg cttacgaaca
     2581 tgagatcttc gccgagctga ttatcctcac tcagctggca ccatttactc caggtccagt
     2641 cctggactcg gtccagcgca caggcaggct gctgacagtt gaagaaggaa ccctgccgct
     2701 gggctggagc gcagagatca ttgcggaaac agcagccgcg cttggatcac gcttactcgc
     2761 cagccggcgc ctggcagcct tgaattcacc cataccagcc agcggtccac tcgaatctga
     2821 gacactccca gatatcacaa cgatcatccg ggcagcccaa tcaatggtat aactggagcc
     2881 agcggagcca acctgcatga attcaccaat acccgttacc actcccctgc tcaaccccaa
     2941 tgagccagaa gcttccctgg ccgcactcca tgtccagaat ggacaacacg ttacaaccgg
     3001 cgacccgctg tttacgctgg agacaaccaa gtctgctgct gatgttcagg cggaagaggc
     3061 agggtatgtg accggaatac aggtccacat aggtgataca gtccaggccg gggaaatcct
     3121 ctgctatctg gccgattcgc cggaatggac tccagccgag cctgccgtcc agcttacccc
     3181 gcaaactgac caaccagttc ctgaaggctt gcgaattacc caccctgccc ttgagctggc
     3241 ccgccagagt cttgtcgatc tcagccagct tcccagggat cagctgatca cggaaacgac
     3301 tctgcgcgcc taccttacgc cccactctgg cgagaaaaca gagtattcac tgccccctac
     3361 cagcgtcgac agcaccgccg tgatcattta cggcggaggt ggacacgcga aagccttgat
     3421 cgatgtcatc cgggcgatgg gcgtttaccg gattgccggt gtattggatg acaatccgtc
     3481 cgtccagggc gagattctcg gtgtgccagt gctgggtggg gctgaatagc ttcagcttct
     3541 ttatcagcag gggatccggt tagccgttaa tgccgtcggt gggatcggca atatcaggat
     3601 ccgcgtccag attttcgagc gcctggctca agccgggttc gcttttccac tcctgattca
     3661 tcccagggct gtcgtggaag cgagcgcgaa cctgagcgcc ggtgggcagg tattccccct
     3721 ggcctatgtt ggcagcgagg tgaaggccgg attcgggtcg atcatcaata ccggggcgat
     3781 tatctcccac gaatgcatca taggcgattt tgtaaacatt tctcccgggg cgatcctggc
     3841 tggccaggtg cagattggga atggggctct cgtcggtatg ggggcgacgg tcaatttgca
     3901 agtaacgatt ggagccgggg cgcgcatcgg caacggttca acagtgaaat ccgatgtgcc
     3961 cgcaggcggt gtggtccgcg ccggcgggat ttggccggca taaaaacgta atgggagctt
     4021 actccttgca aacttcatcg aaacccacaa taccggcaat accggttcca atgtcttctc
     4081 ctgaccttag cgacgcagaa cgccaggccg ttacagctgt cttgaatacc cctgttctga
     4141 gcatgggaaa agagatccag gcattcgaag cagccctcca ggagcttacc ggttggcagc
     4201 atgcgattgc cgtcaattca ggcacatccg gtctgcacct ctgcattcga gcggcgggtg
     4261 tcggcatcaa cgacggacag actggtgata tggtcctcac cacccctttc tccttcgttg
     4321 cttcgaccaa tgtgatcctg tacgaacagg ctacaccgat ctttgtcgat gttgatccca
     4381 tcaccggcaa tattgaccct gcgctggctg tccaggcaat caaggaactg gcttctggca
     4441 gtcaagcagc ccggcgctgg ctgcctcgta aaggttggaa agaaggtggg cagctcaaag
     4501 ccatcctccc ggtcgatgta tttggccaac ctgctgacat ggatcctttg gtccagac
//
LOCUS       DATMXK010000388         2430 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12408, whole genome shotgun sequence.
ACCESSION   DATMXK010000388 DATMXK010000000
VERSION     DATMXK010000388.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2430)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2430)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2430
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12408"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(377..1045)
                     /locus_tag="VN363_07255"
     CDS             complement(377..1045)
                     /locus_tag="VN363_07255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84346.1"
                     /translation="MSFTNVLLALPVWWINGVLAMLYWSLAHLSEFAALLAGLAILLG
                     FDPWLQQRASQRPRRQGRGEVQTARPNAQYFTLATLLAWLLVSLSSRFPVPLIGACLW
                     WVGLMGVLAVPEERFNQLWWAKAGILAYAGLVLILRFGLAVLGEVSPADWAAVVGSRL
                     DAQVALTSTRGNIATIGMLFTFVLYPLGYTGMLLNRFLRNPKPLYNIGLEASDVLQRL
                     RTRQ"
     gene            complement(1042..2406)
                     /locus_tag="VN363_07260"
     CDS             complement(1042..2406)
                     /locus_tag="VN363_07260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84347.1"
                     /translation="MKTHSRFLLGLLAVCLILAILVHPILSVLAQDDESAWGEFLNPD
                     GSINWANLTYLGEASEPADWMSIDLPGGIQVPLGDAVYNRYLTPSGNVLVLPSPVTLF
                     MTFLNPEQSGFAANMPEMLGSGHSVLAMLAADYVDVADLQALGYIDPAEFFRAVIEGR
                     ENIWSVVSPNFLIELTRMTLDAGYLVTALWLYLSGGDCNAIPGGCPPGFGLTPTPGDS
                     GDDPTPQPAACPAPSISFGDVSASGGQTEPLKPVVVGQDPEKRGADVEVRIAIPPVIF
                     TWYEAHQTRSCEYVSSGTGSGCPGPGNRFDRVIEADGDRTSWSSSMENNSNWNVDTET
                     ECIEHVEIFPDYLDFASLSINLSGESRNWILTDLAQAYPGAHLKRPDWNFAFQGPGSL
                     GGASVVSFFQLIPNIQFADPGVYNLRLNGRTTGTLVSAPRPFDLPLNQFQVDLLRVTL
                     IEAP"
BASE COUNT          576 a          715 c          716 g          423 t
ORIGIN      
        1 ctgtcaccag ggtagtcagg gcagccagga ttacgaccaa accagccagc aggatgatgg
       61 aaaaggcaag cggccaggca aaactaaaga aattcgtcgg catcgatgct cctttcggtc
      121 taaatctttc tgcaggttta cccgcagggg gcactcggat ggcgcctgtg cgaccccatc
      181 cgagtgcagc gacgaagtgc tactcccgtg caattggacc gcagtccacg tgcaaactcc
      241 tgcgcagagg caggtggtca ctcaatcgag gattgagtaa cacatttcaa gggcgaagcc
      301 agtgcaaggg ctctgaaaat ccgggtattt gattcccatt tggcttcgtc cctgatatgt
      361 caggttaata aggtgcttac tgccgtgtgc gcagtcgctg gagtacatcg ctggcttcca
      421 ggccaatgtt gtacagaggt ttcgggttgc gcagaaagcg attgagcagc atcccggtgt
      481 aaccaagcgg gtagagtaca aaggtaaaca acatgccgat ggtggcgata ttgccgcgcg
      541 tgctggtcaa cgctacctgg gcatccagcc ggctgccgac caccgccgcc caatctgccg
      601 ggctgacctc tcccaacact gccagcccga agcgcaggat caagaccaat ccagcgtagg
      661 cgaggatgcc ggccttcgcc caccatagct ggttaaaccg ctcttctggc acagccagga
      721 ctcccatcag gccaacccac cacagacagg cgccgatcaa cggaacggga aagcgcgagg
      781 agagtgacac caacagccag gccaatagag tcgccagggt gaagtactgg gcgttcgggc
      841 gagctgtttg cacttcgccc cgaccctggc ggcgagggcg ctggctggcg cgctgctgaa
      901 gccatggatc gaaccccaac aggatggcca ggccggccag caaggcagcg aattcagaca
      961 ggtgcgcaag tgaccagtac agcatggcaa gcactccatt gatccaccaa accggtaatg
     1021 ccagcaggac atttgtgaag ctcatggcgc ttcgatcagg gtcacccgca gtagatcgac
     1081 ctggaattgg ttcaggggca ggtcgaaagg ccgcggcgcg gacaccaggg tgccggtggt
     1141 gcgcccattc aagcgcaggt tgtacacacc ggggtcggcg aattgaatat tggggataag
     1201 ctggaagaag gagaccacac tcgcccctcc caggctgccc ggcccctgga aagcaaaatt
     1261 ccaatccggg cgcttgaggt gtgcgcctgg ataagcctga gccaggtcgg tcaggatcca
     1321 attacgcgac tcgccggaca agttgatgct cagcgaagcg aaatccagat aatcggggaa
     1381 tatttcgaca tgctcgatgc actcggtctc ggtgtcgaca ttccagttgg aattgttctc
     1441 catcgaggag ctccagctgg tgcggtcacc atccgcctca atcacccggt caaagcgatt
     1501 gcccggtcct gggcaaccac tgccggtccc actggaaaca tattcgcagg aacgggtctg
     1561 gtgagcctcg taccaggtga agatcaccgg cgggattgcg atacgcactt ctacatccgc
     1621 gccgcgcttt tcaggatcct gcccgaccac caccggcttg agcggctcag tctgtccacc
     1681 tgaggcagac acatcaccaa aagagatcga tggggcagga caggcagccg gctgaggagt
     1741 agggtcatcg ccagaatcgc cgggtgtggg ggtcaggccg aaacctggag ggcagccgcc
     1801 ggggatcgca ttgcagtctc cgccgctcag gtagagccat agggcagtca ccagatagcc
     1861 agcgtccaag gtcatgcggg tgagctcgat caggaaattc ggtgagacca cagaccagat
     1921 attctcacgc ccctcgatca ccgcccggaa gaactccgcc ggatcgatgt aacccagggc
     1981 ctgcagatcc gccacgtcaa cataatcggc tgccagcatg gccaacaccg aatgcccgga
     2041 accaagcatc tcaggcatat tggcagcaaa cccggattgc tcagggttca agaaggtcat
     2101 gaacagggtg accggggagg gcagcaccag cacattaccg ctgggggtga gatagcgatt
     2161 gtaaaccgca tcgcccagcg ggacctggat gccacctggc agatcaatgc tcatccaatc
     2221 cgccggctcg gatgcctcac ccagataggt cagattggcc caattgatcg agccatccgg
     2281 gttgaggaac tcaccccagg cgctctcatc gtcctgagca agcacagaca ggattgggtg
     2341 caccaggatc gccagaatca ggcagacagc taacagacca agcagaaatc gagaatgggt
     2401 tttcattgct ctcctccact gtatgccacc
//
LOCUS       DATMXK010000389         5311 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_22953, whole genome shotgun sequence.
ACCESSION   DATMXK010000389 DATMXK010000000
VERSION     DATMXK010000389.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5311)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5311)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5311
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22953"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..344)
                     /locus_tag="VN363_07265"
     CDS             complement(<1..344)
                     /locus_tag="VN363_07265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84348.1"
                     /translation="MVESHRLPASMAAITPPPIIVRIHSAPPILTEGSEMEFTMWLGL
                     LPLRWLARMESISPTGFTDRQLRGPFAEWIHRHTFRAVDAGTTEVLDEINLRLRIHPI
                     WGLIGAGMRLGLP"
     gene            complement(386..1714)
                     /locus_tag="VN363_07270"
     CDS             complement(386..1714)
                     /locus_tag="VN363_07270"
                     /inference="COORDINATES: protein motif:HMM:NF014375.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWQ84349.1"
                     /translation="MTANQPAYNLKAVIHETGLTPATLRAWERRYGVPRPQRSSGGHR
                     LYTLEEIEMLKWLVARQSEGLSISQAIELWRSQGRLPPARIASSRSPRVESGSTITML
                     DQLREEWIAACLAFDEPAAELALSQSLALAAPEVVTTEMLQKALAELGEGWYAGKVSV
                     QQEHFASALAARRLNALLAAAPPPSRPARLLAACPPGEMHDLALLMLAFSLRWQGWDV
                     VYLGANVPLDRMAATLQSARPRLVLSVAQTLPGAAALKELAHFVSEHSVALAYGGGIF
                     NQIPDLTLSIPGYSLGQELNAVPKKIESLLTHLPALPTPLPLSPEYAAALAGFKEKDA
                     LIIAAVNNVLQNGQINPRHLDEANLNFTHSIVSALALGDLRFLDHSVGWLDGLLANHG
                     LSPKLAEEYYAVYRRAVEQLLGAQGQPIIDWLANIHLAGSGSFDQKGQRS"
     gene            complement(1940..2376)
                     /gene="rnpB"
                     /locus_tag="VN363_07275"
     ncRNA           complement(1940..2376)
                     /ncRNA_class="RNase_P_RNA"
                     /gene="rnpB"
                     /locus_tag="VN363_07275"
                     /product="RNase P RNA component class A"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00010"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00010"
     gene            2516..2794
                     /gene="rpsT"
                     /locus_tag="VN363_07280"
     CDS             2516..2794
                     /gene="rpsT"
                     /locus_tag="VN363_07280"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559742.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000312 - plastid small ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S20"
                     /protein_id="HWQ84350.1"
                     /translation="MANIKSAIKRNKQNEKRRLRNRLYVGKARTTIKKARLAMEGTDL
                     AAARAATLTAISALDKAAEKGILHRNNASRRKGRLMRHLAALEKQAAG"
     gene            2975..4879
                     /gene="argS"
                     /locus_tag="VN363_07285"
     CDS             2975..4879
                     /gene="argS"
                     /locus_tag="VN363_07285"
                     /EC_number="6.1.1.19"
                     /inference="COORDINATES: protein motif:HMM:TIGR00456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004814 - arginine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006420 - arginyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="arginine--tRNA ligase"
                     /protein_id="HWQ84351.1"
                     /translation="MIETTRHVIIQAQAGQRFRNLHQAQDIPAPMFTTERQAVENQIH
                     SYCSAQGLPGPGELLWSPVPFSGEWGISTSFFHLAATVARQQAAAGERVNVAQLAQEI
                     ASGIAAHLGAPAGFSHVEAVRGYLNLYYSTAEYSQRVVDTVLAQGADFGKGALKNERI
                     MVEYAQPNTHHSFHIGHFRNAVLGEALSRLVEFAGFETIRASYPGDIGLGVITVVWAY
                     NKFYRGLEPEGIHERGQWLLKIYVEATSLLEPREGETEAEKAQRETYDAERREVYRKW
                     DEGDPEVRQIWQMTREWSLQELRDILRLIGVPIDVWFFESQMDTSAKGIVDELIQRGV
                     ADDERLNGGAVIVRIDEKLGLTKEKYRTNVLLRSDGTTLYLTKDLALAKVKFEQYHVD
                     RSLYVIDVRQSLYMQQVFKILELWGFPQAQMCYHLGYGFVSLPEGAMSARRGRVVSFM
                     EVATEAFRRVQNVITEKNPDLAEADRQQVAEQVGLGALAYAMLAVDNNKDIVFEMDAA
                     LNFDGHTAPYIQNAHVRANSILKKAGGLPATATFDYALTPHEIELIDLISRFPAAVQQ
                     AALEYKPLAIASYAYDLAKAFHSFYHVVPVVQTEQETIRFARLRLVAAARQTLANALH
                     LLVIQAPDVM"
     gene            4974..>5311
                     /locus_tag="VN363_07290"
     CDS             4974..>5311
                     /locus_tag="VN363_07290"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWQ84352.1"
                     /translation="MSPIRTLIMGAAGRDFHNFNVFFRDNPDYQVVAFTATQIPDIEG
                     RTYPAELAGKLYPAGIPIHAESELVDLIRQHQVEQVIFAYSDVPHAVVMHKASIVNAA
                     GADFRLMGTRL"
BASE COUNT         1178 a         1555 c         1508 g         1070 t
ORIGIN      
        1 ggtagaccca ggcgcattcc tgcgccgatc aatccccaga ttgggtgaat gcgcaggcgc
       61 aggttaattt catccagcac ttcggtagtt cctgcatcga ctgccctgaa tgtatgcctg
      121 tggatccatt cggcaaacgg ccctcgcagc tgccggtcgg tgaatccggt tggcgatatg
      181 ctctccatcc gggccagcca gcgcaggggt aagaggccca gccacatggt gaattccatc
      241 tcactgccct cggtaaggat tggtggagcg gagtggatgc ggacaatgat tggcgggggc
      301 gtgatggcag ccatgcttgc cggcaagcga tgggattcaa ccacacggga gagcggagcc
      361 tgtaccagaa accggtgacg ataaatcaac tccgctgccc cttttgatca aaactgccgg
      421 aacccgccag atggatattg gccagccaat cgataattgg ctgcccctga gcgccaagca
      481 gctgctccac agcccggcgg taaactgcgt aatattcctc cgccagcttt ggtgagagac
      541 catgattggc gagcaaacca tccagccagc ctaccgaatg gtcgagaaaa cgaagatcgc
      601 ccagcgccag ggctgagaca attgagtgcg taaaattgag attggcctca tccaggtggc
      661 gaggatttat ctggccattt tgcaaaacat tattgaccgc cgctatgatc aacgcgtcct
      721 tctccttaaa ccctgccagc gcagcagcat actcgggaga aagcggaagc ggtgtgggta
      781 gggcagggag gtgggtgagc aagctctcaa tcttctttgg gaccgcattg agctcctgcc
      841 ccagggaata tcccgggatg ctcagggtaa gatccggaat ctggttgaag atcccaccgc
      901 catacgccag ggcaaccgaa tgctcactca caaaatgggc cagctctttc aaagctgccg
      961 ccccgggcag ggtctgcgca accgacagaa ccagacgcgg cctcgccgat tgcagggtag
     1021 ctgccattcg atccagaggg acattggcac ccagatagac cacgtcccaa ccctgccagc
     1081 gcaggctgaa agccagcatg agcaacgcca ggtcgtgcat ctcgcccggc gggcaggctg
     1141 ccagcaggcg cgcaggtcga gaaggcggtg gagctgcggc gagcaaagca tttaaccggc
     1201 gggctgccag ggctgaggca aaatgctcct gctggacgct gacttttccg gcataccagc
     1261 cttcgccaag ctcagccagc gccttttgca gcatctcggt agtcacaact tcgggagctg
     1321 ccagcgccag gctttgactc aatgccagct cagcggctgg ttcatcaaaa gccaggcagg
     1381 cagcaatcca ctcctcccgc agctggtcca gcatggtgat cgtcgatcca gactcaaccc
     1441 ggggtgatcg actggaagcg atccgagccg ggggaaggcg accctgactg cgccacagct
     1501 caattgcctg gctgatactc agtccttcgg actggcgcgc caccaaccat ttgagcattt
     1561 cgatttcctc gagggtgtac aggcggtgcc cgccggaaga gcgctgcggt ctgggtaccc
     1621 catagcggcg ctcccaggcg cgcagggtgg ctggggtaag gccggtttca tggatgacgg
     1681 cttttaagtt gtacgcgggc tggtttgcag tcatggagtt cctttccagc agagcaagct
     1741 aatcaaaact ttatgcctgt ccaggctatt ctgattgctg aggtcgaaag cgataatttc
     1801 tggaatgcaa gataatcata tgttcatata ttgtacaact tttgcacaga atgacaaggg
     1861 aagaaaggct aatatttgat taatatcgat caaagggatg gcaaagtcac caggcgatgt
     1921 ttttacagga ggtggacctt ccccaggcca gcctataagc cgcattctgt gagtggaagc
     1981 agcgcttcca cccggcgatc atctctctgg gccaggcgtt accgcctggc tctagcagcc
     2041 tacccggaac ctgggtgtgc aaggcacaca cggagacgag ccgcctcctc ccgctgtgcg
     2101 ggcagattcc tgcttggcct tgctccgggc gggggttacc tggccgcggc ggttacccgt
     2161 cgcgccggtg agctcttacc tcaccttttc accctcgcgc cagcttccca aagggaagcc
     2221 agcgcactgt gtctctgtgg cccgatccgg aaagtaacct gtaatcgcct gagcgattac
     2281 acgccctctc cccgggtgtt acccgacgcc ctgctctctg gagtgcggac tttcctcgac
     2341 gcgcctgaag cgcaccgcga tcgcccggcc aacctgggga aattaatcat actataaaac
     2401 cattagattt ctgtttgcat tgagaatttc tggacaaaac accaaccttc gtgtaaaata
     2461 acatgccttg ggtcacagcc gtgatccatc tgagctattt tttcggagga agactttggc
     2521 taacattaaa tctgccatca agcgcaataa gcagaatgaa aagcgccgcc tgcgcaaccg
     2581 cctctatgtg ggtaaggcgc gcacaaccat caagaaagcc cgcctggcga tggaaggcac
     2641 cgatctggct gcagcacgcg cagcgacctt gaccgccatc agcgccctgg ataaggctgc
     2701 cgagaagggt attctgcaca gaaacaacgc cagccgccgc aagggccgcc tgatgcgcca
     2761 cctggccgcg cttgaaaagc aagccgccgg gtagcatatt tttctgcttg cgcatgcgct
     2821 gagctgatcg tacgtatcag catccatacc agcgttcgcc atccacatct gaaatcccca
     2881 gaccagcggg agcggaacag gctcccgctg gttttgcgcg cttaataaca tccatcaagt
     2941 ctgctctggg cgatttcaag atcagcgcct gctcatgatc gaaacgacac ggcatgttat
     3001 aatccaggcg caggcgggtc agcgtttccg caatctccac caggcacagg atatcccagc
     3061 acctatgttt accaccgaaa gacaggccgt cgaaaaccag atccacagct attgttctgc
     3121 ccagggctta cctggtccag gggaattgct ctggtcgccg gtgccattca gtggagaatg
     3181 gggcatcagt acctctttct tccatctggc cgcgaccgtg gctcgccagc aggctgccgc
     3241 tggcgaacgg gtaaatgttg cccagctggc ccaggagatt gccagcggaa ttgccgccca
     3301 tctgggagcc ccggctggat tcagccatgt cgaggctgtc cgtggttacc tgaacctgta
     3361 ttattccacc gccgaataca gccagcgggt ggttgatacc gtcctggcgc agggcgctga
     3421 tttcggcaag ggcgctctaa aaaacgagcg catcatggtg gagtacgccc aacccaacac
     3481 ccaccattct tttcacatcg ggcattttcg caatgcggta ttgggcgaag ccctctcccg
     3541 gctggtagag ttcgccggct tcgagaccat ccgcgcctcc taccctggcg atattggcct
     3601 gggggtgatc accgtggtat gggcatataa caagttctat cgcggcctgg aaccggaagg
     3661 catccacgag cgcggccagt ggctgttaaa gatctatgtc gaagctacca gcctgctgga
     3721 gccgcgtgaa ggcgagaccg aagccgaaaa ggctcagcgc gaaacatatg acgccgagcg
     3781 ccgcgaagtg tatcgcaaat gggatgaagg cgacccggaa gtccgccaga tctggcagat
     3841 gacgcgtgaa tggagcttgc aagagctgcg cgatatcctg cgcctgatcg gcgtgccgat
     3901 cgatgtgtgg ttctttgaaa gccagatgga cacctcggcc aaagggatcg tcgatgagct
     3961 gatccagcgg ggcgtcgccg atgacgagcg cctcaacggt ggggcggtga tcgtgcggat
     4021 cgacgaaaag ctgggattga caaaagaaaa ataccgcact aacgtgctcc tgcgcagcga
     4081 tggcaccacc ctttacctga ccaaagacct ggcgcttgcc aaagtcaaat tcgagcagta
     4141 tcacgtagac cgctcgttat atgtgattga tgtgcgccag agcctgtaca tgcagcaagt
     4201 gttcaagatc ctggaattat ggggcttccc ccaggcgcag atgtgctatc acctgggata
     4261 tggttttgtc agcctgccgg aaggcgcgat gagcgcccgg cgcgggcggg tagtgagctt
     4321 catggaagtc gccacggaag ctttccggcg ggtgcagaac gtgattaccg agaaaaaccc
     4381 cgatcttgcg gaagctgacc gccagcaagt ggctgaacag gtggggcttg gcgcgctggc
     4441 gtatgccatg ctggcggttg acaacaataa ggatatcgtc tttgagatgg atgccgcgct
     4501 taattttgac gggcataccg ctccatacat ccagaatgcc catgtgcgcg ccaacagcat
     4561 cctgaagaaa gccggcggcc tgccagctac cgcgaccttc gattacgctc tgacacccca
     4621 tgagatcgag ctgatcgacc tgatctcccg cttccccgcc gcagtgcagc aagcagcgct
     4681 ggaatacaaa ccgctcgcga tagccagcta tgcctacgat ctggccaaag ccttccacag
     4741 cttttaccat gtggttccgg tggtccaaac ggagcaagaa accatccgtt ttgcccgctt
     4801 gcgtctggtg gcagccgccc gccagaccct ggccaatgcc ctgcacctgc tcgttatcca
     4861 ggcgccagat gtgatgtaag ttcgaaggcc cggaagctgt atcaagcgct tccggtccct
     4921 ttttatgttt cagcaacacc aaaaccaaag cctcaaagga gattttcgaa cccatgtcac
     4981 cgatccgcac tttgattatg ggcgccgccg ggcgcgattt ccacaatttc aacgtttttt
     5041 tcagggataa cccggattac caggtggtgg ctttcacagc tacccagatc ccggatattg
     5101 aaggacgcac ctaccccgcc gaattagcgg gcaagctgta ccccgcgggc atcccgatcc
     5161 atgccgaaag cgagctggtc gatttgatcc gccagcacca ggtggaacag gttatcttcg
     5221 cctactcgga tgtaccccat gcggttgtga tgcataaagc ctccatcgtg aatgcggctg
     5281 gggcggattt ccgcctgatg ggcacccgcc t
//
LOCUS       DATMXK010000390         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_154499, whole genome shotgun sequence.
ACCESSION   DATMXK010000390 DATMXK010000000
VERSION     DATMXK010000390.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154499"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..297
                     /gene="tsf"
                     /locus_tag="VN363_07295"
     CDS             <1..297
                     /gene="tsf"
                     /locus_tag="VN363_07295"
                     /inference="COORDINATES: protein motif:HMM:NF013085.4"
                     /note="EF-Ts; functions during elongation stage of protein
                     translation; forms a dimer; associates with EF-Tu-GDP
                     complex and promotes exchange of GDP to GTP resulting in
                     regeneration of the active form of EF-Tu; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="elongation factor Ts"
                     /protein_id="HWQ84353.1"
                     /translation="AAAPRYIRAEDIPEIEMEREREVARQRAIEEGKPEAIHDKIIAG
                     RLEKFKDEACLLRQTYIRDEGVKIEQLLMQNVAAIGENIVIRRFARWELGESTK"
     gene            376..1101
                     /gene="pyrH"
                     /locus_tag="VN363_07300"
     CDS             376..1101
                     /gene="pyrH"
                     /locus_tag="VN363_07300"
                     /EC_number="2.7.4.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005328724.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0033862 - UMP kinase activity [Evidence
                     IEA];
                     GO_process: GO:0015949 - nucleobase-containing small
                     molecule interconversion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UMP kinase"
                     /protein_id="HWQ84354.1"
                     /translation="MANEIKYKRILLKLSGEALAANGSSGIDPLRAEDVAERVQEIHD
                     MGIDVAIVIGAGNLWRGTRGLERGMDRATADYMGMLATVMNALALMDAMERRGLVTRV
                     QSAIEMHAVAEPYIRRRAIRHMEKGRVVILGGGTGNPYFSTDTAAALRAMEIGAEVVI
                     KATKVNGVYDSDPMLNPNAVRYEHLTYMDIISRRLEVMDTTAITMCMEHNLPILVLNL
                     WDPESLRKALRGEKVGTLVDANE"
     gene            1265..1822
                     /gene="frr"
                     /locus_tag="VN363_07305"
     CDS             1265..1822
                     /gene="frr"
                     /locus_tag="VN363_07305"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558782.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008135 - translation factor activity, RNA
                     binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome recycling factor"
                     /protein_id="HWQ84355.1"
                     /translation="MLKEIFQEADNRMKGAIQSLEDDLAGIRTGRANPSLIERLSIDY
                     YGNPTPLIQLATISVPEARQLLIRPFDASTLKTIERAILASDLGLNPSNDGKSIRLIL
                     PALTEERRRDLVKVVHTRVEEARVSARNVRRDCIKDLREFEQEKMISEDDLERGEEDL
                     QKLTDKYIQDITSIGERKEKEIFEI"
     gene            1842..2597
                     /gene="uppS"
                     /locus_tag="VN363_07310"
     CDS             1842..2597
                     /gene="uppS"
                     /locus_tag="VN363_07310"
                     /EC_number="2.5.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558781.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenyl diphosphate synthase"
                     /protein_id="HWQ84356.1"
                     /translation="MNSPETKDLPLKIPTHVAIIMDGNGRWALGRGLPRLAGHRAGTE
                     NLRRIIEASTEFGIHYLTIYAFSTENWGRPVEEVQGLLHILEDVIDKELQELYDQGVQ
                     IRHIGRLDRLDPELREKVIHAVDFTRQNDTLVLNVAFNYGGRDEIVCAIQRIIQDGIP
                     PAEITDELVSQYLFTAGVPDPDLIIRTSGELRGSNFLIWQGAYCEWYFTPTYWPDFDK
                     EQLRLALIEFSQRERRYGRISLEEKGDLSEHAG"
BASE COUNT          646 a          692 c          721 g          576 t
ORIGIN      
        1 gctgccgcac cgcgctacat ccgcgccgaa gatattcctg aaatcgaaat ggaacgcgaa
       61 cgcgaggtcg ctcgccagcg cgccatcgaa gaaggcaagc cagaagcaat ccatgataag
      121 atcatcgctg gccgtctgga gaaattcaag gatgaggcat gcctcttgcg ccagacttat
      181 atccgcgacg aaggcgtgaa aattgagcag ctcttgatgc aaaatgtcgc cgcgatcggt
      241 gaaaatatcg tcatccggcg tttcgcccgc tgggagctgg gtgaaagcac gaaataatca
      301 gcagaacaaa aggaccaacc ccacaggttg gtccttttgc tgcaggctaa taaacctcga
      361 gaaagggtgt gtcgaatggc aaacgaaatt aaatacaaac gcatcttact caaactgagc
      421 ggggaagcat tggcagccaa tgggtccagc gggattgacc cgctgcgggc tgaggacgta
      481 gcggagcgcg tccaggaaat ccatgatatg ggcatcgatg tcgccatcgt gattggggct
      541 ggaaacttat ggcgggggac gcgcgggctc gagcgcggca tggatcgcgc cactgccgat
      601 tatatgggca tgctggcgac ggtgatgaat gcgctggcgc tgatggatgc catggaacgc
      661 cgcgggctgg ttacccgggt ccaatccgcg attgagatgc acgcagtagc cgagccttat
      721 atccgccggc gggcgatccg ccacatggaa aaaggtcgag tggttatcct cgggggtggc
      781 accggaaacc cctatttttc cacagacact gcggctgccc tgcgggcgat ggagattggc
      841 gctgaggtgg ttatcaaagc taccaaagtc aatggcgtgt atgactctga tcccatgctc
      901 aatcccaatg ctgtccgcta cgagcacctc acctatatgg atattatcag ccggcgtttg
      961 gaagttatgg ataccaccgc catcaccatg tgcatggaac acaacctgcc aattctggtc
     1021 ttgaacttat gggatccaga atccttacgt aaggcattgc gcggcgaaaa agttggcact
     1081 ctggtcgacg caaacgaata agctatctca cacagccgct tacctcctgc cccggttgat
     1141 acgctgagcc ggggcttttt cccccttttc gctcacacaa ttttcgagta tcctataggt
     1201 tggcgtacaa ttaggccggg ttactatttt tcacagaaat gatcgtgaca ttgtggaggc
     1261 ctccatgcta aaagagatat ttcaggaagc tgataaccgc atgaaaggcg caatccagtc
     1321 gcttgaggac gacctggcag gtattcgtac cgggcgagcc aatccatccc tgatagaacg
     1381 tctctcgatt gattattatg gcaatccgac cccactgatt cagcttgcca ccatcagtgt
     1441 ccccgaagct cgccagttgt taatccgccc ctttgatgct tcgaccttaa aaacgatcga
     1501 acgagccatc ctggcatctg acctgggatt gaatccgagt aatgatggaa aatcaattcg
     1561 cctcattctc cctgcgctga cggaagagcg ccggcgtgat ctggtcaaag tcgttcacac
     1621 ccgggtggaa gaggcccggg tttcagcccg caatgtacgc cgggattgca taaaagacct
     1681 gcgcgaattt gagcaggaaa aaatgatctc tgaagatgac cttgagcgtg gtgaagaaga
     1741 tctgcaaaaa ttgacggata agtacatcca ggatattacg agcattggcg aacgcaaaga
     1801 gaaggagatc ttcgaaatct aaagtggtac agacaccagc catgaactcc ccggaaacga
     1861 aagatctgcc cttaaagatc cccacccatg tcgcgatcat catggatggc aatggtcgtt
     1921 gggctctggg acgcggttta ccgcgcctgg cgggtcaccg cgccggcaca gagaatttgc
     1981 gccggatcat tgaagcctcc actgagttcg gtattcacta tctgacaatc tacgccttct
     2041 caactgaaaa ctggggccgc cccgtagaag aggtccaggg cttattacac attcttgagg
     2101 atgttatcga caaagagctc caggaattgt acgatcaggg cgtccagatc cgtcatattg
     2161 gtcgcctcga ccggcttgat cctgagctcc gcgaaaaggt tatccacgcg gttgatttca
     2221 cccgccaaaa tgataccctg gtgctcaatg tggcatttaa ttacggtggt cgcgatgaaa
     2281 tcgtgtgcgc catccagcga attatccagg acggtattcc gcctgccgag atcacggatg
     2341 agctggttag ccagtattta ttcaccgccg gagtccccga cccggatttg attatccgca
     2401 cttcgggtga gctgcgcggc agcaatttcc tgatctggca gggcgcatac tgtgaatggt
     2461 actttacgcc aacttactgg cctgattttg ataaagagca gctgcgcctg gctttgatcg
     2521 aattcagcca gcgcgaacgc cgctacgggc ggatatctct ggaggaaaaa ggggacctgt
     2581 ctgagcatgc tggctaaacg cttgctggtt gcagccatcc tgctgccagt aggcc
//
LOCUS       DATMXK010000391         4844 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_115741, whole genome shotgun sequence.
ACCESSION   DATMXK010000391 DATMXK010000000
VERSION     DATMXK010000391.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4844)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4844)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4844
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_115741"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VN363_07315"
     CDS             <1..378
                     /locus_tag="VN363_07315"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84357.1"
                     /translation="AFILYLRPDLAHMERVVDETEFISTPNYYMDWVEGGALIANPPW
                     YDDTLTGAYGAGSLATAENGALWLAAAAREKAGHILEIHEQYERRLARREEHARSQEH
                     ARTPEQARPSEHAHQNDPNNPTI"
     gene            392..1054
                     /locus_tag="VN363_07320"
     CDS             392..1054
                     /locus_tag="VN363_07320"
                     /inference="COORDINATES: protein motif:HMM:TIGR02370.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="corrinoid protein"
                     /protein_id="HWQ84358.1"
                     /translation="MSSELFAAMTRSIIDGEVDDAAELARQALAQGIDPLEVINEGYV
                     PGVQHVGQEFSCGNAFLPELVMAGEAMKAAIAVLEPEMARRGVERTLEGRVVIGTVKG
                     DIHEIGKTLVGTLLTAHGFEVYDLGVDVEVTSFVEKARAVNADLVGLSALLTTTMVVQ
                     RKVIQALEESGLRPGVQVMVGGAPVTRQWAAEIGADGYAEDATGAVQLARELMAKARS
                     QE"
     gene            1057..2505
                     /locus_tag="VN363_07325"
     CDS             1057..2505
                     /locus_tag="VN363_07325"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HWQ84359.1"
                     /translation="MSKEILSISNPRLRLEILSDADVQRLHTATLDVIANTGVRFPSR
                     RALEIWEAHGAAVDHATQIVRAPGELIESALKTAPPAYSLAARLPEQDLPLDGNHVFV
                     GTDGCGVEIIDLDDGQRRTSRLADVVDIARIADALPEIGFHWVPLSAQDCPPETRGLH
                     ELLAIWANSTKHAQTESIYSAAEATLAVEMASAIAGGRESLRQRPVLSLMQCTAPPLG
                     QDGGSVDAALIAAEAGLPVGFMTMTASLTTAPATLAGNLVVGNAEVISALALIQLAFP
                     GAPVYYAAAQTASDLRTGSYTGGGPEDFLFGAATNRLADFYNVPLSMGSFATGAKEPN
                     WQAGLENSLSTFMACVSMSDMLLGVGLLHGSRIWSYEQMLLDCEIYSIIHKMMAGIPV
                     DDESIALETIHAVGPGGNFLSQKHTRRHMRELWLPRYLDRRPYNAWEEQKDDGRDWAR
                     QRARQLRAEHQPVPLESGLEKELQRMIRTLEA"
     gene            2508..3968
                     /locus_tag="VN363_07330"
     CDS             2508..3968
                     /locus_tag="VN363_07330"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HWQ84360.1"
                     /translation="MQPMLRLLSEDMVQQVLDEAFSLLENTGILVQSPAARQLLAENG
                     AILSGDVARLPQGLVLDALVSAPHEFFLYNHHAEIVVRYGGDTVQFDPGSSGVNIFDP
                     HTCQHRPAVTADLVQLIQVTEMLPEYAAQSTSVVCSDVPKEIGDLYRLYLVLLYGSKP
                     VITGAFNRQNTQAMIDMLALYAGGREALRKRPLAVFDVCPTPPLIFSPFAAESLIDLA
                     QAGIPAELISMPLAGAGSPVTILGSIVQHAAETLAGITLHQLAGPGAPVVWGGAPAVF
                     DMRFGTTPMGAIETAMLDVGYAQVGKSLNLPTHTYLGASDSKLVDYQAGLESGISAIL
                     GALGGINMISGAGMLDFLAAQCVEKLVADAEAIRMAQRLLRGIQPLTETYALELFAGI
                     NFKADFLKQKATRQLFSKEQSLPSAVIDRGSLRAWDENGRSETVERAGKRAAELLAAY
                     RRPEAPAGVEAELRQLVGGLARQSGMDRLPELPDPD"
     gene            4008..>4844
                     /locus_tag="VN363_07335"
     CDS             4008..>4844
                     /locus_tag="VN363_07335"
                     /inference="COORDINATES: protein motif:HMM:NF018012.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0015948 - methanogenesis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trimethylamine methyltransferase family protein"
                     /protein_id="HWQ84361.1"
                     /translation="MQRLEILTFPEIEAIHQASLRILAETGIILGQPEALALLQEAGA
                     ARAGKFLRLPPDLVEWALSQVPPCFSLHGRSGSTITLGEGHLHWHNLGGAAQIYDPLT
                     DNVRPALVHDLAAAARLLDALPGVDSITPFFTPQDAPPHALALAMYRHTLPHTGKPLH
                     SPGAQTALEVKYLAEMAGVVGNPPEVLSMGISPISPLTFPDDVVEALLASARLGLPTG
                     PLPCPTAGMTAPLSLAGALAQQNAEVLAAIVILQVARPGLPVYYCGRLSMMEPRSGLS
                     VWG"
BASE COUNT          882 a         1629 c         1474 g          859 t
ORIGIN      
        1 gccttcatcc tttacctgcg ccccgatctg gcccatatgg agcgagtggt ggatgaaact
       61 gaattcatct cgactcccaa ttattatatg gactgggtag aaggcggcgc cctgatcgcc
      121 aatcctccct ggtatgatga caccctgacc ggagcctacg gcgccggcag cctggcgacc
      181 gctgaaaacg gtgccctctg gctggctgcc gccgcccggg aaaaagccgg gcatattctt
      241 gaaatccacg aacagtacga gcgccgcctt gcgcggcgag aagagcacgc tcgcagtcaa
      301 gagcacgctc gcactccaga gcaagctcgc ccttcagagc acgctcatca gaacgatccc
      361 aacaacccga cgatttaacc aggaggtctc tatgagttcg gaactattcg ccgccatgac
      421 ccgcagcatc atcgatggag aagtagacga cgcggctgag ctggcgcgcc aggcactggc
      481 ccagggcatc gaccccctgg aagtgatcaa cgaaggttat gtccccggcg tccagcatgt
      541 cgggcaggaa ttcagctgcg ggaatgcctt cctgcccgag ctggtgatgg ccggcgaagc
      601 catgaaagcc gccatcgccg tgctcgagcc agaaatggcc cgccgcggcg tggagcgcac
      661 cctggaaggc cgggtcgtga tcggcaccgt gaaaggcgat atccacgaga tcggcaaaac
      721 gctggtgggc accctcctca ccgcccatgg tttcgaagtc tatgatctgg gtgtggatgt
      781 cgaggttacc agttttgtcg agaaagcccg ggcggtaaat gccgatctgg tcgggctgag
      841 cgccctgctg accaccacca tggtcgtcca gcgcaaggtg atccaggcac tggaagaatc
      901 cggcctgagg ccgggcgtgc aggtcatggt cggcggcgcg ccggtcaccc gccagtgggc
      961 ggccgagatt ggggccgatg gctacgccga agacgccacc ggggctgtcc agctcgcccg
     1021 cgagctcatg gccaaagccc gcagccagga ataacaatga gtaaagaaat cttatccatc
     1081 agtaacccgc gcctgcgcct ggaaatcctg tcggacgccg atgtccagcg gctgcacacc
     1141 gccaccctgg atgtgatcgc caacaccgga gtgcgttttc catcccggcg cgccctggag
     1201 atctgggaag ctcacggcgc cgcagtggat catgccaccc agatcgtgcg cgcccctggc
     1261 gagctgatcg aatccgccct caaaacagcc ccaccggctt acagcctggc ggcccgcctg
     1321 cccgaacagg acctgcccct ggatggcaat catgtcttcg tcggcacgga tggctgcggg
     1381 gtggagatca ttgacctgga cgacggccag cggcgtactt cccggctcgc ggatgtggtt
     1441 gatatcgccc gcatcgccga cgcccttccg gagattggct tccactgggt gccgctctca
     1501 gcccaggact gcccgcccga aacgcgcggc ctgcatgagc tgctggcaat ctgggccaat
     1561 tccaccaagc acgcccagac cgaatcgatc tattcggctg ctgaagccac gctggcggtt
     1621 gaaatggcca gcgctattgc cggcgggcgt gaatctttgc gccagcgccc ggtgctgtcc
     1681 ttgatgcagt gcaccgcgcc ccctctgggg caggacggcg gcagcgtgga tgccgcctta
     1741 atcgccgctg aagccggact gccggtcgga tttatgacca tgaccgccag cctgaccacc
     1801 gctccggcga ccctggcagg caacctggtg gtgggcaacg ccgaggtcat ttccgctctg
     1861 gccctgatcc agcttgcctt tcccggggcg ccggtttatt acgctgccgc ccagaccgct
     1921 tccgacctgc gcaccggttc ctacaccggc ggtgggccgg aagacttcct gttcggagcc
     1981 gccaccaacc gcctggcgga tttttacaac gtcccgctct cgatgggttc cttcgccacc
     2041 ggcgccaaag agccgaactg gcaggccggg ctggaaaaca gcctgtccac cttcatggcg
     2101 tgtgtgagca tgtcggatat gctcctgggt gtcggtttgc tgcacggcag ccgcatctgg
     2161 tcctatgagc agatgctcct ggactgcgag atttattcga ttatccacaa aatgatggca
     2221 ggcatcccgg tcgacgatga atcgatcgct ctggaaacga tccatgccgt gggtccgggc
     2281 ggtaacttcc tgagccagaa acacactcgc cgccacatgc gcgagctctg gctgccgcgc
     2341 tacctggacc gccgcccgta caatgcctgg gaagaacaaa aagatgacgg ccgcgattgg
     2401 gcgcgccagc gcgcccgcca gctgcgcgcc gagcaccagc cggttcccct ggaatccggc
     2461 ctggaaaaag aattacagcg tatgatccgc accctggagg cttagccatg cagcccatgc
     2521 tcagattact cagcgaagac atggtccagc aggtcctgga cgaagccttc agcctcctgg
     2581 aaaataccgg catcctggtc caatcccccg ccgctcgcca gctgctggca gaaaatggag
     2641 ccattctgag cggtgatgtc gcccgcctgc cccaggggct ggtcctggac gccctggtca
     2701 gcgccccgca tgagtttttc ctgtataacc atcacgccga gatcgtggtg cgctacggcg
     2761 gtgataccgt gcagttcgat cctggctcat cgggagtgaa tatcttcgac ccgcacacct
     2821 gccagcaccg tcccgccgtc actgccgacc tggtacagct gatccaggtg accgagatgc
     2881 tgcccgaata cgccgcccag tctacttcag tcgtatgcag cgacgtcccc aaagagatcg
     2941 gcgaccttta ccgcctttac ctggtcctgc tctatggcag caagccggtg atcaccgggg
     3001 cattcaaccg ccagaatacc caggcgatga tcgacatgct ggcgctgtac gccggcgggc
     3061 gggaggcttt gcgcaaacgc cccctggccg tcttcgatgt ctgcccgacg ccacccctga
     3121 tcttcagccc atttgccgcc gagagcctga tcgacctggc ccaggccggt atcccggccg
     3181 agctgatctc catgcccctg gccggggcgg gatcgccggt caccatcctg ggctcaatcg
     3241 tccagcatgc cgccgagacc ctggcaggca ttaccctgca ccagctggcc gggcctggcg
     3301 caccggtggt gtggggcggc gccccggcag tcttcgacat gcgcttcggg acgacgccca
     3361 tgggagcaat cgagaccgcc atgctcgatg tcggctatgc ccaggtaggc aaatccctca
     3421 acctgcccac gcatacttac ctgggggcca gcgattccaa gctggtcgat taccaggccg
     3481 ggctggaatc gggaatcagc gccatcctgg gcgccctggg cgggatcaat atgatctccg
     3541 gagccgggat gctcgatttc ctggcggccc agtgcgtgga gaagctggtc gcggatgccg
     3601 aggcgatccg gatggcccag cgcctgctgc ggggcatcca gccgctcacc gagacctacg
     3661 ccctggagtt gtttgccggg atcaatttca aagccgattt tctcaaacaa aaggccaccc
     3721 gccagttatt cagcaaggag cagagcctgc cttcggcggt catcgaccgc ggctcgctgc
     3781 gcgcctggga tgaaaacggt cgctcggaga cggtggagcg cgccggtaaa cgcgccgctg
     3841 agctgctggc ggcttaccgg cgcccggaag cccctgccgg tgtcgaagct gagctgcgcc
     3901 agctggttgg cggcctggct cgccaatccg ggatggaccg gctgcctgaa ttaccggatc
     3961 cagattaagc gccggatcac ccggcgtgtg gagaaagctg atcgatcatg cagcggctcg
     4021 aaattcttac tttccctgag atcgaagcca tccaccaggc cagcctgcgc atcctggcgg
     4081 aaaccggaat tatcctgggc cagccggagg cgctcgccct gctgcaggag gccggtgcag
     4141 ctcgcgccgg gaaattcctg cgcctgccgc cagacctggt ggaatgggcg ctgagccagg
     4201 ttccgccctg cttcagcctg cacgggcgca gcggaagtac aatcacgctc ggagaaggac
     4261 atttacactg gcacaacctg ggcggcgcag cgcaaattta tgacccgctc acggacaatg
     4321 tccgcccggc tctggtccat gacctggcag ctgccgctcg cctgctcgac gccctgccgg
     4381 gggtggattc gatcaccccc ttcttcaccc ctcaggatgc acctccccac gccctggcgt
     4441 tggcgatgta ccgccacacc ctgccgcaca cgggcaagcc gctccacagc ccgggagcgc
     4501 aaactgccct ggaggtgaaa tacctggccg aaatggccgg cgtggtcggg aatccgcccg
     4561 aagtgctcag catgggcatc tccccgatca gcccgcttac atttccggat gatgttgtag
     4621 aggctctcct ggcctccgcc cggctcggcc tgcccaccgg tccgctgccc tgccccaccg
     4681 ccgggatgac cgccccgctc tcgcttgccg gggcgttagc ccagcagaac gctgaggttc
     4741 tggccgcgat cgtcatcctg caagtcgccc ggcccggcct gccggtctat tactgtgggc
     4801 ggctctcgat gatggagccg cgctctggct tatccgtatg gggg
//
LOCUS       DATMXK010000392         2675 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_77982, whole genome shotgun sequence.
ACCESSION   DATMXK010000392 DATMXK010000000
VERSION     DATMXK010000392.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2675)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2675)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2675
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_77982"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1284)
                     /locus_tag="VN363_07340"
     CDS             complement(<1..1284)
                     /locus_tag="VN363_07340"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560461.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpaF family protein"
                     /protein_id="HWQ84362.1"
                     /translation="MSLLKRIEQGQGNQPATPVENSGADGASRLMNLQARRGTPSAST
                     AQRDTYLDLKARVQNRLLSELDPSMDITRVSEVRSTIQELFEQVLSEENIVLTRPERQ
                     RLFEQISAEILGFGPLQPLLEDDTISEIMVNGAKNIYIERGGKITRLPISFESDEHVM
                     RIIDRIVAPMGRRIDESSPYVDARLPDGSRVNAVIPPISLVGPVLTIRKFFKNPITLD
                     QLVQVGSITPDALTFLKACVESRLNIIISGGTGSGKTTLLNILSQFIPDDERIVTIEN
                     AAELQLRQEHVVTLESRPPNIEGRGEVTIRQLVINALRMRPDRIIVGEIRDEAAVDML
                     QAMNTGHDGSMTTAHSNSPRDSLARVETMAMMSGMDLPVRAIREQVASAIDLIVHEER
                     MRDGTRKIVNITEVSGMEGDVITMTDIFVFEQSGYC"
     gene            complement(1310..2563)
                     /locus_tag="VN363_07345"
     CDS             complement(1310..2563)
                     /locus_tag="VN363_07345"
                     /inference="COORDINATES: protein motif:HMM:NF013793.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ84363.1"
                     /translation="MVDSDKIRVLIVDDIAETRENLRKLLQFEKDVEVIGVARSGREA
                     IDQARETKPDVVLMDINMPDMDGITATELIRRNNTVTQIVILSVQGDPNYMRRAMLAG
                     ARDFLTKPPAIDELTGAIHRAGKMAHDERMKVSMRLPSGGEAGTTLSPLASQGKIITV
                     YSPKGGAGVTTLAVNLAMTLRNEDTFVTIVDGDLQFGDVAVFLNEQSKTSVVDLGARA
                     DELDPEFVDEVMITHAASGVKILAAPDRPEEAESINGEQFAKVLKYLRQMYAYIVIDT
                     DSALNDITLAALDTSDLVILVTTQEIPAIKNCRLFLDLAIAMKLDRRRILFVMNRFDK
                     RIGITPEKVGESFKTPISAILPFDERVVIPSVNRGVPFMLGDRSKPISKGFLSLAEAI
                     RQRLVELSTTPAEALASNGKKFGKR"
BASE COUNT          559 a          753 c          759 g          604 t
ORIGIN      
        1 acagtacccg gactgctcga atacgaagat atcggtcatg gtgatgacat cgccttccat
       61 accactgact tcggtgatat tgacaatctt gcgcgtgcca tctcgcatcc gctcctcgtg
      121 cacaatcaga tcgatggccg aggccacctg ctcacggatg gcacggacag gcagatccat
      181 gccggacatc atggccatcg tttcaacgcg agccagggaa tcgcgcgggc tgttcgagtg
      241 ggcagtcgtc atcgagccat cgtgacccgt gttcatcgcc tggagcatat ctacagccgc
      301 ttcatcccga atttcaccga cgataatccg gtcggggcgc atacgcaagg cgttgatcac
      361 cagctggcgg atggtcactt cgccgcgacc ttcgatattc ggagggcggg attcaagggt
      421 aaccacatgt tcctgcctca gctgcagttc ggccgcgttt tcgatggtga caatgcgctc
      481 atcatcggga ataaactgcg acaggatatt gagcagggtc gtcttaccag aaccagtacc
      541 ccctgagata atgatattca ggcgggattc cacacaggct ttcaagaagg tcagcgcgtc
      601 cggggtgatc gagccaacct ggaccagctg gtccaacgtt atgggattct tgaagaattt
      661 ccggatggtc agcaccggcc ccaccagcga aatgggcggg atgacggcat tgacgcgcga
      721 gccatcgggc aaacgggcat ccacatatgg gctggattca tcaatccgcc ggcccatggg
      781 cgccacgatg cggtcgatga tgcgcattac gtgctcatcg ctctcaaagg agatgggcag
      841 ccgggtgatt ttgccgccgc gttcgatata gatattcttg gcaccattga ccataatttc
      901 tgagatggtg tcatcttcca gcaggggctg caggggacca aaacccagga tctccgctga
      961 gatctgttca aacaaacgct ggcgttctgg ccgggtgagg acaatgtttt cctcggagag
     1021 cacctgctcg aagagctctt gaatggtgga gcgcacttcc gatacccggg taatatccat
     1081 gctcgggtcc agctcggaaa gcaggcgatt ctggacgcgg gctttcaggt ccagataggt
     1141 atcgcgttgg gcagtggaag cggagggagt accccgcctc gcctgcaggt tcattaaccg
     1201 ggaagcaccg tccgccccac tgttttccac gggggtggcg ggctggtttc cctggccttg
     1261 ttcgattcgt tttagcagag acatgatagc cttcctatct tctcaatgac tagcgcttcc
     1321 caaatttctt accattactt gccaatgctt ctgccggtgt ggttgataat tctaccagcc
     1381 gctggcgaat ggcttcagcc aggctcagga aacctttcga gatcggtttg gagcgatcac
     1441 ccagcataaa tggcacaccg cggttcacag atgggattac tacccgctca tcaaagggca
     1501 ggatggcaga gatgggtgtt ttgaaacttt cgcccacttt ttccggggtg atcccgatcc
     1561 gcttatcaaa gcggttcatc acaaataaga tccggcggcg atccagcttc atcgcgatcg
     1621 caaggtccag aaacagccgg cagtttttga tcgccggaat ttcctgtgtg gtgactaaga
     1681 ttaccaggtc gctggtgtcg agggctgcca gggtgatatc gttgagcgca gaatcggtat
     1741 cgatgacgat ataggcgtac atctggcgca ggtatttgag cacttttgcg aactgctcac
     1801 cgttgatgct ttcagcctct tcgggccggt ctggcgctgc caggatcttc actccggaag
     1861 cagcatgcgt gatcataacc tcgtccacaa attcgggatc gagctcatcg gcgcgcgcac
     1921 ccagatcgac cacactggtt ttactctgtt cattgaggaa taccgccaca tccccaaact
     1981 gcaggtcgcc atccacgatg gtaacaaaag tatcttcatt gcgcagggtc atcgccaggt
     2041 tcacggccaa tgtggtaacc cctgcgccac ctttggggct gtaaacggta atgatcttgc
     2101 cctgggaggc cagcggcgag agcgtagtac cggcctcacc cccgctgggc aggcgcatgc
     2161 tgactttcat gcgttcatcg tgagccatct tgcccgcccg gtggatcgcc ccggtcagct
     2221 cgtcgatcgc cgggggtttg gtcagaaagt cgcgtgctcc ggccagcatc gcccggcgca
     2281 tataattcgg gtcaccctgg acggatagga tcacaatctg ggtgacggtg ttgttgcgcc
     2341 ggatcagctc ggtggcggtg atcccgtcca tatcgggcat attgatgtcc atcaggacga
     2401 catccggttt cgtttcgcga gcctggtcga tcgcctcccg gccggagcgg gcaaccccaa
     2461 tcacttcgac atctttttcg aactgcaaga gcttgcgcag attctcgcgc gtttcagcga
     2521 tatcatcgac gatcagaacg cgaatcttgt ctgagtcaac catgcatatt cctccgcagc
     2581 tgcccgggat tcagatcagg gttccggggt tggtggagca tcgttgggca gcaccggcga
     2641 aaccagatca tcgacgcgcg gatcaatacc gaaag
//
LOCUS       DATMXK010000393         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_49791, whole genome shotgun sequence.
ACCESSION   DATMXK010000393 DATMXK010000000
VERSION     DATMXK010000393.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_49791"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1031)
                     /locus_tag="VN363_07350"
     CDS             complement(<1..1031)
                     /locus_tag="VN363_07350"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWQ84364.1"
                     /translation="MNNLERFNKAINWEPTDRILTYDFLDNRHILTRYGDFDETRQYS
                     FEELMAVNGTAWKRLGVDVTRFVYDPVNHWMGSKITNWIRFFGVDPENWGVSQSGGTA
                     WISKRPFKTLKELEKHMPNLPVYEDVRDWYQPSIRQMQQVLGEYDVVMIGAVEGPITD
                     AYSYMDMELFAQGIYDAPELVAHIMDCTGMFSAQIARAYAEVTEARLLFMGEDIAGST
                     GPIFNPKFVRKEGLPRWKWITGPVKEKGYKFLYHTDGRYGNFLPLIFDDLGADGLNPI
                     ERNGCNDIFAIRAAYPDKLLFGNVCCIHTLPHGSLGDVEDETLELIEKIGPQGGIFIG
                     SSSEVHDACL"
     gene            complement(1083..2063)
                     /locus_tag="VN363_07355"
     CDS             complement(1083..2063)
                     /locus_tag="VN363_07355"
                     /inference="COORDINATES: protein motif:HMM:NF013431.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84365.1"
                     /translation="MNGLILQSTWHGLPAWVLENEALRTIVVQELGAKLVSLFDKRSQ
                     KEWLADPGDRAVKKVAYGAVFTDQDMSGWDEMFPTIVACEYPLPGRHAGALLPDHGEV
                     WALPWKLEPAPTGSLTLSVEGIALPYRLTRRLAYCAADVLQMDYGLENLGDEPMPYIW
                     AAHPQFLCGEAAEIRFPFQVSEVCNTVDASWGWGEPETIFTWPAAVRPDGTAFRIDHT
                     GPPTLKQARKFYVLPEKRVAWAGLLRQPVQDWLRLDWDPEKVPYLGLWVDEGALSHTT
                     VAAPEPTTGFYDSLVLAWEKKEITVLEPHATQTWTLTVRLGIPGQPFPDE"
     gene            complement(2103..>2738)
                     /locus_tag="VN363_07360"
     CDS             complement(2103..>2738)
                     /locus_tag="VN363_07360"
                     /inference="COORDINATES: protein motif:HMM:NF013381.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004853 - uroporphyrinogen decarboxylase
                     activity [Evidence IEA];
                     GO_process: GO:0006779 - porphyrin-containing compound
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="uroporphyrinogen decarboxylase family protein"
                     /protein_id="HWQ84366.1"
                     /translation="TVTTIFECAWALRGYERMLSDFALNPDLAEQILDIPYRYHLAVA
                     KKLTALGVDMIWTGDDVGAQNAMLISPKMWRKFLKPRMANFIAELKAINPQVKVAYHS
                     DGAIYPIIPDLIEIGLDVLNPIQPASMDPVRLKREYGDQLCFWGSIDEQHTLPFGTAG
                     DVRAEVCTRLETLGKNGGLILGPTHHVQLDTPMENFWALINTTTQTPYPES"
BASE COUNT          610 a          762 c          737 g          629 t
ORIGIN      
        1 agacaggcat cgtgtacctc actcgatgag ccgataaaga tgccgccctg gggaccaatc
       61 ttctcaatca gctccagagt ttcatcctct acatcgccta acgagccgtg gggtaaagta
      121 tggatacaac aaacatttcc aaaaagcagc ttatccggat aggctgcgcg aatggcaaaa
      181 atgtcgttac agccattgcg ttcgatcgga ttaagcccat ccgcgccaag gtcgtcgaag
      241 atcagtggca agaaattccc ataccggccg tcggtgtggt acaggaattt atatcctttt
      301 tctttgaccg ggccggtgat ccatttccag cggggtaagc cttctttacg cacaaatttg
      361 gggttgaaga tcggtccggt ggaaccggcg atatcctctc ccataaataa tagcctggcc
      421 tcggtgactt cggcataggc gcgggcaatc tgggctgaaa acatccctgt gcagtccatg
      481 atgtgagcga ccagctcggg agcatcataa atcccctggg caaataattc catatccatg
      541 tatgaatagg catcggtgat cggtccctcc acagccccga tcatgaccac atcatattcg
      601 cccaatacct gctgcatctg gcggatgctg ggttggtacc aatcgcgcac atcttcatac
      661 actggcaggt tgggcatatg cttttcaagt tctttgaggg tcttgaacgg gcgcttggag
      721 atccaggctg ttccgccgct ctggctgaca ccccagttct ccgggtctac gccaaagaac
      781 cggatccagt tggtaatctt gctgcccatc caatgattta ccggatcgta gacaaagcgg
      841 gtgacatcca cgcccaggcg cttccaggcg gtgccgttaa ccgccatcag ctcttcaaag
      901 ctgtactggc gagtttcatc gaaatctcca tagcgggtca ggatgtggcg gttatccaga
      961 aaatcatacg tcaggatgcg atcggttggt tcccaattaa tagctttgtt aaatcgctca
     1021 agattattca tagcatttat tcctgttggc agctgagatc tggacaatat tcacgagtat
     1081 cgttactcgt caggaaaggg ctgtccggga atccccaggc gcacggtcag ggtccaggtt
     1141 tgagtggcat gcggctccaa gaccgtgatt tcttttttct cccaggctaa tacaaggctg
     1201 tcatagaaac cggtggttgg ctccggcgcg gcaacagtgg tgtggctcaa cgcgccttca
     1261 tcaacccata agcccaggta aggtactttc tctggatccc agtcgaggcg cagccagtcc
     1321 tggacgggct ggcggagcag accggcccag gcgactcgtt tttctggcag gacatagaac
     1381 ttgcgcgcct gttttaaggt aggtgggcca gtgtggtcaa ttcggaaagc tgtgccatcc
     1441 ggacggacgg ccgctggcca ggtaaagatc gtctcaggtt cgccccatcc ccagctggca
     1501 tccaccgtgt tgcacacttc gcttacctgg aacggaaacc gaatttctgc tgcctcgccg
     1561 cacagaaact gcgggtgggc agcccagata tagggcattg gctcatcccc gaggttttcc
     1621 agcccataat ccatctggag cacatctgca gcgcagtaag ccaggcggcg cgtcagccgg
     1681 tatgggaggg caatcccttc cacacttaga gtgagcgagc ctgtcggagc cggctccagc
     1741 ttccagggca gcgcccagac ttcgccatga tccggcagga gcgcaccagc atgtctgccc
     1801 ggcagagggt attcacaagc cacaatcgta gggaacatct catcccaacc gctcatatcc
     1861 tgatcggtaa agactgcacc gtaggcaact tttttcaccg ctctgtcgcc cggatcagcc
     1921 agccattctt tttggctgcg tttatcaaac agggagacca gcttggcacc cagctcttgg
     1981 acaacaatgg tcctcagtgc ctcgttttcc aggacccagg caggcaggcc gtgccaggta
     2041 gattgcagta tcagcccatt catagaggga ttctcttttc gggtgaacag tcttttgcca
     2101 gatcaagatt caggataagg cgtctgggtg gtggtattga tcagggccca gaagttttcc
     2161 atgggtgtat ccagctggac atgatgggtt ggccctaaga tcaacccgcc gtttttgcca
     2221 agggtttcca ggcgggtaca gacttcggcg cgcacatctc ccgccgtgcc gaaaggcagc
     2281 gtgtgctgct cgtcgataga gccccagaaa cacagctggt cgccatactc gcgcttaagc
     2341 cggaccgggt ccatactggc tggctggata ggattgagca catccaggcc gatctcaatc
     2401 agatccggga tgatcggata aatggcgcca tccgaatggt aggctacttt cacctgcggg
     2461 tttatcgctt tcagctcggc gataaaattg gccatccgcg gtttgaggaa tttccgccac
     2521 attttcggcg agatcagcat ggcattttga gcgccgacat catcccccgt ccagatcata
     2581 tctacgccca gggcagttaa tttcttggca actgccaggt ggtagcgata aggaatatcc
     2641 aggatctgct ctgccaggtc agggttgagc gcaaagtcag acagcatccg ctcatagccc
     2701 cgtagcgccc aggcgcactc aaagatcgtc gtaacggt
//
LOCUS       DATMXK010000394         3529 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_117650, whole genome shotgun sequence.
ACCESSION   DATMXK010000394 DATMXK010000000
VERSION     DATMXK010000394.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3529)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3529)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3529
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117650"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..776)
                     /locus_tag="VN363_07365"
     CDS             complement(<1..776)
                     /locus_tag="VN363_07365"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWQ84367.1"
                     /translation="MSRADRIAILLSLIAVVAAYFIAERIFEQIPHLEDEMAYVWQSK
                     AFARGQLTVPTPPEPKSFLVPFVVDYEGRRFGKYPPGWPALLAIGELVGLRGWINPLL
                     SGLGIWLTYRIGKKILGETVGLLAAGLTITSPLFLMNSGMLLSHPLGLVLTAGFCLSW
                     LNAFWKPDHPRPWISTLIGAVCLGWLAVTRPWTAVAVALPFAAHGLYLLIYSKWSTRR
                     RLIAFILVTLAISALVPVWQKAVTGDFTLNPYTLWWEYDKV"
     gene            complement(812..1795)
                     /locus_tag="VN363_07370"
     CDS             complement(812..1795)
                     /locus_tag="VN363_07370"
                     /EC_number="3.1.3.7"
                     /inference="COORDINATES: protein motif:HMM:TIGR01330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008441 - 3'(2'),5'-bisphosphate
                     nucleotidase activity [Evidence IEA];
                     GO_process: GO:0006790 - sulfur compound metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="3'(2'),5'-bisphosphate nucleotidase"
                     /protein_id="HWQ84368.1"
                     /translation="MPAYTPDEILFTLETACQAVLLVQMIQRELVDGTLTKDDRSPVT
                     VADFAAQALVGFRLAQAYPADRLVAEEDSARLKNDPAMLERVTQYLRRQIPEATPDLV
                     AAWIDHNRADGGERFWTLDPIDGTKGFLRGDQYAVALALVVAGEVVLGVLGCPHLNPN
                     AEPVRNSGALFYAARGYGAWAVDLQQSHTAQRLNTSSRNDPSDARILRSFESGHTNTG
                     QIDDFSNWMNTQAAPVLMDSQAKYAVLASGRAEILLRLLSASQPDYREKIWDQAAGSI
                     VLEEAGGRISDLDGIPLDFTTGRSLIHNRGILASNGHLHTSALSALRQIGV"
     gene            complement(1805..3208)
                     /gene="rimO"
                     /locus_tag="VN363_07375"
     CDS             complement(1805..3208)
                     /gene="rimO"
                     /locus_tag="VN363_07375"
                     /EC_number="2.8.4.4"
                     /inference="COORDINATES: protein motif:HMM:TIGR00089.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008172 - S-methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016782 - transferase activity,
                     transferring sulphur-containing groups [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0018339 -
                     peptidyl-L-beta-methylthioaspartic acid biosynthetic
                     process from peptidyl-aspartic acid [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12 methylthiotransferase
                     RimO"
                     /protein_id="HWQ84369.1"
                     /translation="MAGMKQKTFHLVSLGCAKNTVDSDSMSQLLSVSGFQPVLKPSQA
                     QVLIVNTCGFIGPAKAESLSVLRELAEQKKKGQVLIAAGCLTQRYGAEVARLVPGIDG
                     ILGTRRWMDIVDLVLELRRGPHPEPLYHLPETPTVGADEHGALRASVQGGSAYIKIAD
                     GCRRPCAFCAIPLIKGTAVSRPLETIVQEAITLQAAGVREIILIAQDTTDYGHDLGLK
                     DGLAVLLEKLVQAVPQIDWLRILYAYPGYVTDHLIEVMASNPQIVPYLDIPLQHAHPD
                     TLRRMRRPANIEWVHKTLAKMRTAIPNLALRTTFIVGYPGETESEFQALLDFITEIRF
                     DRAGAFQFSFEPGTFSEALGDPVPAELKQERYDRLMTLQQPISLEKNQALVGNILPVL
                     IEGEGDGISVGRSYRDAPEIDGMVVVEGIAPIGEIVPVRITGAMAYDLSGSLLPAYPT
                     PANLIQLNGAIKPTQIS"
     gene            complement(3253..>3529)
                     /locus_tag="VN363_07380"
     CDS             complement(3253..>3529)
                     /locus_tag="VN363_07380"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84370.1"
                     /translation="QGRILAGSAYNFEGEERVELLTSNGAALQIFYNQQDQGILGLFS
                     EAIFIVYTMDGVQTPTPTITPTGTTTPRPSPTPRQTATPPAGPPALP"
BASE COUNT          793 a          960 c         1013 g          763 t
ORIGIN      
        1 actttgtcat actcccacca cagggtatat ggatttaagg tgaaatcgcc tgtcactgct
       61 ttttgccaga ctggtaccaa tgcactgatt gccagcgtga ccaggataaa ggcgattaaa
      121 cgccgccggg ttgaccattt actgtagatg agcaggtata agccgtgggc ggcgaatgga
      181 agggcgaccg cgaccgctgt ccagggtcgg gtaactgcca gccatcccag acagactgcc
      241 ccaatcaggg tcgaaatcca gggacggggg tgatctggtt tccaaaaggc attcagccag
      301 gaaaggcaaa aaccagccgt cagcactaat cccagtgggt gagaaagcag catgcctgaa
      361 ttcatgagga agagcggcga tgtaatggtc agaccggctg ccagcaagcc aaccgtctcg
      421 cccagtattt ttttgccgat ccggtaggtg agccagattc ccagaccaga caacagggga
      481 ttgatccagc cgcgcaggcc aaccagttca ccaattgcca gcagggcagg ccagcccggc
      541 ggatatttcc caaagcgccg gccctcataa tcaacgacaa acggcaccaa aaaacttttc
      601 ggctccgggg gagtcggtac agttagctgg ccgcgggcaa atgccttaga ctgccagacg
      661 taagccatct catcttcaag atgcggaatt tgctcgaaaa tccgctcggc gatgaaataa
      721 gccgccacaa cggcgatcag gctgagcagg atggcgatgc ggtcagcgcg agacatagtg
      781 ggagctcttg ctaaaaatac ctgatgttgg ttcagacgcc tatctgtcgc agggcagaga
      841 gggcagatgt gtgtaaatgc ccgttcgaag ccaggatacc ccggttgtgg atcaacgacc
      901 gccccgtggt gaagtccagt gggatgccgt ctaaatcgct tatccgaccg ccggcttcct
      961 ccaggacgat ggatccagct gcctggtccc aaattttttc acggtagtca ggctggctgg
     1021 cagaaagcag ccgcagcagg atttctgccc tacccgaggc cagaacggca tatttagcct
     1081 ggctgtccat caggacggga gcagcctgag tgttcatcca atttgaaaaa tcatcaattt
     1141 gtcccgtatt ggtgtggccg gattcaaatg agcgcaggat gcgtgcatcg gaaggatcgt
     1201 tcctggagga ggtatttagt ctttgagcag tatgagattg ctgcagatcg actgcccagg
     1261 cgccataccc acgggctgca taaaataatg ctccgctgtt acggactggc tcggcgttgg
     1321 gattcaggtg cgggcagccc aacactccca gaacaacctc ccctgcgact accagagcca
     1381 gagcaactgc atattggtcg ccgcgcaaga aacctttggt gccatcaatc ggatcgaggg
     1441 tccagaatcg ctctccgcca tccgccctgt tatgatcaat ccaggctgct accaggtctg
     1501 gcgtcgcctc cgggatctgg cgccgaaggt attgagtcac tcgttcgagc atcgctggat
     1561 cgtttttcag ccgcgctgag tcttcttcag cgaccagccg gtcggcaggg tatgcctgag
     1621 ccagacggaa gcctaccagt gcctgggcgg cgaaatccgc cacagtcacg ggtgagcgat
     1681 catctttagt cagggtccca tcgaccagct ctcgctggat catctggacg agcagaactg
     1741 cctggcaggc ggtttctaag gtgaacagaa tttcgtcggg agtgtaagct ggcatgatcg
     1801 ttccttacga gatctgtgtg ggttttatgg ctccgttgag ctggatcaga ttggccggcg
     1861 ttggatatgc agggagcagg ctgccgctga gatcgtatgc catggctccg gtaatgcgga
     1921 ccgggacgat ctcgccaatt ggggcgatgc cttcgaccac caccatgcca tcgatctccg
     1981 gagcatcccg gtagctgcgg ccaaccgaaa ttccatctcc ctcgccttcg attaaaaccg
     2041 ggagaatatt cccaaccagt gcctggttct tttcaagtga gataggctgc tgcagggtca
     2101 tcaagcggtc gtaccgttct tgtttgagct cggcggggac aggatcgccg agtgcttcgc
     2161 tgaacgtacc cggctcgaat gagaactgga aggcgccagc gcgatcgaag cggatctcgg
     2221 tgatgaaatc gagtaaggcc tggaattctg attcagtctc acccggatat cccacgatga
     2281 aagttgttcg taaagccaga ttcgggatgg cggttcgcat tttcgccagt gttttgtgca
     2341 cccattcaat attcgccggg cggcgcatgc gtctcagggt gtctggatgg gcatgctgca
     2401 ggggaatatc cagatatggc acgatttggg gattcgaagc cataacttcg atcaagtgat
     2461 ctgtaacata gccggggtaa gcgtagagga tgcgcagcca atcgatttgc gggacagcct
     2521 ggaccagctt ctctaagaga actgccagcc catctttgag gccgagatcg tgaccataat
     2581 cggttgtatc ctgggcgatc agaatgatct cgcgcacccc ggcagcctgc agggtaatgg
     2641 cttcttggac aattgtctcc agggggcggc tgacggctgt ccctttgatc agcggaatgg
     2701 cacaaaaagc gcagggtcgc cggcagccat ccgcaatttt tatgtaggcg gagcctccct
     2761 gaacagaagc gcgcagcgcc ccatgctcat cagccccaac tgtgggagtc tcgggcaggt
     2821 gatacagggg ttccggatgc ggtccacgcc gcagctccag cacgaggtcg acgatatcca
     2881 tccagcggcg tgtgccaagg atgccatcga tgccgggtac cagacgggct acctctgccc
     2941 catagcgctg ggtcaaacaa ccagccgcaa ttaatacctg ccctttcttc ttttgctccg
     3001 ccagttctct taataccgat aaggattctg ccttggcagg accaataaag ccgcacgtat
     3061 tgacgattaa cacctgggcc tgggatggct tcagtactgg ctgaaagcct gacacagaaa
     3121 gcagctggga catcgagtcc gaatccacgg tgttttttgc acagccgaga gacactaaat
     3181 gaaatgtttt ttgtttcata cctgccatac ctggataaaa ttcaccttgc tattacccgt
     3241 ccaacccctg catcagggga gcgccggcgg acctgcggga ggtgttgctg tctggcgcgg
     3301 ggtaggcgac ggtcgcgggg tcgtcgtgcc agtgggtgta atcgtcggtg tcggagtctg
     3361 gacaccatcc atcgtataaa cgataaaaat cgcctcactg aacagcccca ggattccttg
     3421 atcctgctgg ttatagaata tctgcagggc tgcgccattg ctggtaagca attctactcg
     3481 ttcttcgcct tcaaagttat aggcgctgcc agccaggatt ctaccctgg
//
LOCUS       DATMXK010000395         2335 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_12016, whole genome shotgun sequence.
ACCESSION   DATMXK010000395 DATMXK010000000
VERSION     DATMXK010000395.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2335)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2335)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2335
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_12016"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..293)
                     /locus_tag="VN363_07385"
     CDS             complement(<1..293)
                     /locus_tag="VN363_07385"
                     /EC_number="1.8.5.3"
                     /inference="COORDINATES: protein motif:HMM:NF016838.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_process: GO:0019645 - anaerobic electron transport
                     chain [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DmsC/YnfH family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWQ84371.1"
                     /translation="MNLREWALPIYTILMQLAVGTLLVLWLIRRFNLERVGGEAMVRI
                     LRRPVLVMFFTILIAIIGSHLHLSNPLLSFLAVLNIKYSWLSREIVFTVLTFLA"
     gene            complement(290..>2335)
                     /locus_tag="VN363_07390"
     CDS             complement(290..>2335)
                     /locus_tag="VN363_07390"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="histidine kinase"
                     /protein_id="HWQ84372.1"
                     /translation="FYGLSFFGLGLAAYLQLRRGGDFPLRKLLPWLAAFGFAAGAMGW
                     VEMFLASETNPEVVRILDIALMLLQPVSGTLLLIFGWGIVAKLVPLPRWAIFVPGILI
                     VPFAYVITYALSTFVTPSPIEIPIDIWSRYLLYLPGSILAGVGFLRQWAIQRKEGYFD
                     VANLMLGAGIAFLVEAFVMGLVVPAAPYGPASYYNYDRVAYDAFSSEATGVTAQYGLT
                     AWLDYEAILAATGLPIQFWRMLSAFAVTGFVVRGLDVFEAMQKRAVQKLQDERDRAQR
                     AAFDAQIAARQTAESWTEALVRISSQIAELDDVDSILLDIVKSACKLLDADFMGLALV
                     EGEIPRLMLKYYAESKAAKPVESPLPVENPLLLEVLDQQAAYHSTSGEPAERLQGVCL
                     SCGLTAGALAAVPLKMDTISIGTMWVARGEQRVFTETDLIWLECLADQAVIAIQHGLM
                     TSQLQSLSVTEERTRIAREMHDGLAQVLGYMNVQVQTLDAYLQQGKLDRLQVKLDQMR
                     EAVNQAHADVREKILSLRTTLSTEKGVIPAIEEYLEEFGIQAQIATHFENQVQGELDI
                     ASLAEVQLVCILQEALTNVRKHAQAQHVAVVLSKQGPTEREYVRLVIQDDGAGFTKVV
                     HKSSFGLQIMRERAQSVNGSLEVHSIPGEGTQVTCAIPCLGRERLAKSSARLEAGKP"
BASE COUNT          535 a          722 c          644 g          434 t
ORIGIN      
        1 gcaaggaaag tcaggacggt gaatacgatc tcccggctca gccaggaata tttgatattc
       61 aacaccgcca aaaaggacag cagcgggttg ctcaggtgca ggtgtgagcc aatgatagcg
      121 atcaggatgg tgaagaacat caccagcacc ggccggcgca gaatgcgaac catcgcctct
      181 ccaccaactc gctccagatt gaagcggcga atcagccaca ggaccagcag cgtgcctacc
      241 gcaagctgca tcaagatggt ataaattggc agcgcccatt cacgcagatt catggttttc
      301 cagcctccag cctcgcgctg gatttcgcca gtctttcccg gcccaggcag gggatggcgc
      361 aggttacctg cgtcccttcg cccgggatcg aatggacttc caggctgccg ttcacgctct
      421 gggcgcgctc acgcatgatc tgcaaaccaa aactgctttt gtggacgacc tttgtgaaac
      481 ctgcgccgtc atcctggatc accaggcgga catactccct ttccgtcggg ccttgcttgc
      541 tcaagaccac cgccacgtgt tgtgcctggg catgcttacg cacgttggtg agcgcctcct
      601 gcaggatgca gacgagctgc acttcagcca gggaggcaat atccagctca ccctgcacct
      661 ggttctcaaa atgggtggca atctgcgcct ggataccgaa ttcctccaga tactcctcaa
      721 tcgccggaat aacccctttt tcagtggaca gggtggtgcg caggctcaag atcttctcac
      781 gcacgtcggc atgtgcctgg ttcaccgcct cacgcatctg gtccagcttg acctgcaggc
      841 ggtccagctt gccctgttgc aggtaggcat ccagggtctg cacctgcaca ttcatatacc
      901 ccagcacctg ggcgagacca tcgtgcattt cgcgggcgat acgggtgcgc tcttcagtaa
      961 cagataaaga ctgcaactgg gatgtcatca gtccatgctg gatggcaatt accgcctggt
     1021 cagccaggca ctccagccag atcaggtcag tctcggtgaa cactcgctgt tcgccgcgtg
     1081 ccacccacat agttccaatc gatatggtgt ccattttcaa aggcaccgcg gccagcgccc
     1141 cggcggtcag gccgcagctt aagcacaccc cttgcaggcg ctccgcaggc tctccagacg
     1201 tagagtggta agctgcctgt tgatccagga cctccagaag aagggggttt tccaccggaa
     1261 gcggagattc aaccggctta gcagctttag actcggcgta gtatttcaac atcagacgcg
     1321 gaatctcccc ctccaccagc gccagtccca tgaaatcggc atccaacaac ttgcaggcgc
     1381 tctttacgat atccagcagg atgctatcca catcgtccaa ctcggcaatc tggctgctga
     1441 tgcgtaccaa ggcttcggtc caactctcag cggtttggcg ggcggcgatc tgagcgtcga
     1501 aggctgccct ttgcgcccgg tctcgctcat cctggagctt ttgcacggcg cgtttttgca
     1561 tggcctcaaa gacatccagg ccgcgcacaa caaagccagt aaccgcaaac gctgagagca
     1621 tcctccagaa ttgaattggc aagccggttg cagccaggat cgcctcataa tccagccagg
     1681 cggtcagccc atattgggca gtcacaccag tggcttcact gctgaaagca tcgtaagcta
     1741 cacgatcgta gttatagtaa gacgccgggc catacggcgc cgctgggacg accagcccca
     1801 tcacaaaagc ctccaccagg aaggcgatcc cggcccccag catcaggttg gcgacatcga
     1861 aataaccttc cttgcgctgg atagcccact ggcgcagaaa tcccactccc gccaggatgc
     1921 tgccgggcag gtagagcagg tagcgggacc agatatcgat ggggatctcg atcggggagg
     1981 gggtcacaaa ggtggataaa gcataggtaa tgacatatgc aaaggggacg atcaagatgc
     2041 cggggacaaa gatggcccag cgcggcagcg gcacaagctt ggcgacgatt ccccagccga
     2101 agatcaaaag cagggtgccc gaaaccggct gaaggagcat gagcgcaata tccaggatgc
     2161 ggacaacctc gggattcgtt tcgctggcca ggaacatctc cacccagccc atcgccccgg
     2221 cagcaaatcc aaatgccgcc aaccagggca gcagcttgcg caggggaaag tcgccgcccc
     2281 ggcggagctg caggtaggct gccagcccca gcccgaagaa agacaggcca tagaa
//
LOCUS       DATMXK010000396         2351 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_60894, whole genome shotgun sequence.
ACCESSION   DATMXK010000396 DATMXK010000000
VERSION     DATMXK010000396.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2351)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2351)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2351
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_60894"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            531..2303
                     /locus_tag="VN363_07395"
     CDS             531..2303
                     /locus_tag="VN363_07395"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ84373.1"
                     /translation="MKKISWMFTILVLLSLVLAACAQATQVPTEPPVEATEPPAATEP
                     PAEVTEPPAEATEPPAEATEPPAATEPPSDERKVATFIWTQEFDTMNPLYSNMWFTLI
                     TDQLWLAYPWDFDDQNNPHPVLVTEMPSVENGGISEDATTITLNLRDDITWSDGTPLT
                     ADDFIFTWEMAIDPNNTVAEVAPYDLLTSVEGPDPQTVVMNFAEPYAAWAGTMWHGLL
                     PAHILRPVYEADGTLDNAEWNRNPTVGVGPYLFAEWESGSFARFVANENFWLGRPAID
                     EIFFQFVPDDASQVAALKAGTGDLGTFIAYSDIPGLQDVGVNILTVFSGYNEGWYINL
                     NPETAHPAVLDERVRQALAYGFDRFTLAEDLLLGLTQPAATFWDNTPYAFPEAEPYPY
                     DPEMANQLLDEAGWVDSNGDGTRDKDGVELVLTYGTTTREIRQSTQAVVQQQYADIGV
                     GVELLNYDSDIFFGGYGDGGPAATGQLDISQWSDTSNYPDPDLAYWLCSEIPTDEEPT
                     GTNWQGICDEELDGLFQLQATQVDFAERQATFHKISKLMFDKVYWLGLWQDPDTWAVG
                     PRLQNVKISGATPFFNIHEWDLTP"
BASE COUNT          520 a          694 c          627 g          510 t
ORIGIN      
        1 ccggtggaaa ccctggaagc tgcccacaca gattgatttg aggcgggcga actgaccccc
       61 ctgattgcga gtatataggg tagacagcca ctttatgcat ggcgctacaa tcttgattaa
      121 gtccttgaca ctcaccttac cttttgttat aaagaacgat aattggcctt taccaaggcc
      181 ggcaaatgat caattcaaat agcaatcttg ctccgctcac gatccagccc gggccagaca
      241 gatccaactc tgccgcaggt gcccgccctg ctcatcgcgt gggcgttttc atatctggct
      301 gcctgcttca aaaacatttc gattgacatt ccggagaatg tctttattac ccgtctgctg
      361 cagatttcag gtgaacggaa aaaggaggtc agcccaaatt ttctccccgc acatcagcta
      421 ccgagctctg caaggtgttt tcaccctgca gcctgcctgc cgggattgaa tttcccgctg
      481 gcaggagtac attcgaaccg aattggattc gactattgga ggagtaggcg atgaaaaaaa
      541 ttagttggat gttcaccatc ctggtcttgc tgtctctggt gttagccgct tgcgcccagg
      601 caacccaggt acccactgaa ccacccgtgg aggccaccga gcctccagcc gcaaccgaac
      661 cccccgctga agtgacagaa ccgccggcag aagccacgga gccgccagcc gaggcgactg
      721 agccaccggc agcaaccgaa ccgcccagcg acgagcgcaa ggtcgccact ttcatctgga
      781 cacaggaatt cgataccatg aatcccctgt attccaatat gtggttcacc ctgatcaccg
      841 accagctctg gctggcctat ccctgggatt ttgacgatca gaacaaccct catcctgtcc
      901 tggtgactga gatgccttcg gtcgaaaatg gcggcatctc tgaggatgca accacgatca
      961 cgctgaatct gcgggatgat atcacctggt cagatggcac gcctctgacc gccgatgatt
     1021 tcatcttcac ctgggagatg gcgattgatc ccaacaatac cgtcgcggaa gtcgccccct
     1081 acgacctgtt aacttcggtc gaaggtccgg atcctcagac cgtggtaatg aactttgccg
     1141 aaccctacgc agcctgggcc ggcacgatgt ggcacggact gcttcctgcc cacattctgc
     1201 gcccggtata tgaagccgat ggcactctgg acaacgccga gtggaatcgc aacccgaccg
     1261 tgggcgtggg tccatatctt ttcgccgaat gggaatcagg cagttttgcc cgctttgtgg
     1321 ccaacgaaaa cttctggctg ggtcgcccgg caatcgatga gatcttcttc cagttcgtgc
     1381 cggatgatgc ctcgcaggta gctgctctga aagccggaac cggcgatctg gggacgttta
     1441 tcgcctactc agatattcct ggtctgcagg atgtcggcgt aaatatcctg actgtattct
     1501 cgggctacaa cgaaggctgg tacatcaacc tgaatcccga aactgctcat ccagcagtcc
     1561 tggatgagcg cgtccgccag gcgctggcct atggctttga ccgctttaca ctggctgagg
     1621 acctgctttt gggcctgact cagcctgcag ccaccttctg ggacaatacg ccttacgcct
     1681 tccccgaggc agaaccctac ccctacgacc ctgaaatggc caaccagctc ctggatgaag
     1741 ccggctgggt ggacagcaac ggcgatggca cccgcgataa ggacggcgtg gagctggtcc
     1801 tgacctacgg gacgaccacg cgtgaaatcc gccagagcac ccaggctgtc gtccagcagc
     1861 agtatgccga tatcggcgtc ggtgtggagc tcctcaatta cgactcggat atcttcttcg
     1921 gtggttacgg tgacggtgga ccggctgcca ccgggcagct ggatatctcg cagtggtcag
     1981 acacttcgaa ctacccggat cccgatctgg cctactggct gtgcagtgag atccccacgg
     2041 acgaagagcc caccggtacc aactggcagg ggatctgtga tgaagagctg gatggtctgt
     2101 tccagctcca ggcgacccag gtcgattttg ctgaacgcca ggcgaccttc cacaaaatca
     2161 gcaagttgat gtttgacaag gtttattggc tgggcctctg gcaggatcca gatacctggg
     2221 cagttggacc ccgcctgcaa aacgtgaaga tttctggtgc aaccccattc ttcaatattc
     2281 atgaatggga tttgacgccg taattcaggc gaatctcgtc aaacttgagc cggggctgga
     2341 cgaagacagc c
//
LOCUS       DATMXK010000397         3100 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_31543, whole genome shotgun sequence.
ACCESSION   DATMXK010000397 DATMXK010000000
VERSION     DATMXK010000397.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3100)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3100)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3100
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_31543"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1390
                     /locus_tag="VN363_07400"
     CDS             <1..1390
                     /locus_tag="VN363_07400"
                     /inference="COORDINATES: protein motif:HMM:NF012401.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ84374.1"
                     /translation="TKPPEPTALPEPTVTPELAAEVALDLVGQEESLAFTLEELKALP
                     MAEGQAGTKSSTGKITPPIDYQGVLLTDLLAEVGGLTEDLGVNIVAEDGYMMTFSYNQ
                     LTLGDFITYDPATGAEKRVEGPLQVLVAYARGGELLDPKGDGVLRLAIISPKNDQVTD
                     GHWSVKWVRKIELKPVGDEWNLAMKGAVEGAIDRNSFQSCSAPGCHQVNWTDGENQVW
                     TGVPLWTLVGWADDLIQHSGPAYFDALADAGYTIEIIAADGYSINLDSVRVKRNNELL
                     VAYQVNGAPLPDKYFPLRLVGAGVSDNAESIGAISEIDLIIDPAVAASVPPLLPVGEA
                     VMAALAEPAIAGDLNIGGLVNNPLGLMEEDLRGVSPVKITVEHPKQGPTEYEGVYLNL
                     LLTLAGVQGGGTQLVLTAGDGYTTTIDLLQARACTDCLVAFTEAAGELTLVMPGFDSS
                     AWVKDITHIEVK"
     gene            1741..2964
                     /gene="wtpA"
                     /locus_tag="VN363_07405"
     CDS             1741..2964
                     /gene="wtpA"
                     /locus_tag="VN363_07405"
                     /inference="COORDINATES: protein motif:HMM:NF024921.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tungstate ABC transporter substrate-binding
                     protein WtpA"
                     /protein_id="HWQ84375.1"
                     /translation="MQTKKVIQRILQAAISGCLIASCSSATAYPPQETASRAPTTAEG
                     QLATENQPMKPGAETLELGETPTAPSPTPVVQRMESGVLRVFCAGSLILPFSELEKAF
                     EAANPEIDVQTEFHGSIQVMRHVTDLHEAIDVVATADRALIPMLMYATDDPDTGEPYA
                     DWYLHFAGNKVGLAYTTESRRAGEINAQNWYSILADPGVRFGLADPRIDPSGYRTLMA
                     LRLAEVYYGQPGIFQDLMGSKFSYPIGIFKEAGYTEISVPEILEPKLDSSLALRGASI
                     QLVALLQSGDLDYAFEYESVIRQHGLELLELPEAVNLGSAAQAETYRQVEVKLDFQRF
                     AKIQPVFRGEPIGYGITIPSNAPEPGLAEQYIQFLLSEEGRGIMEAYYHPVFEQVGCD
                     GVENMPEVLRGVCLE"
BASE COUNT          670 a          810 c         1038 g          582 t
ORIGIN      
        1 gaccaaacca ccggaaccga ctgcgctacc ggaaccgaca gtaacacctg agctggcagc
       61 tgaggttgcc ctggatctgg ttgggcagga agagagcctg gcctttaccc tggaggagct
      121 taaggcactg ccaatggctg agggccaggc cgggacgaaa tcgtccaccg ggaagatcac
      181 cccaccgatc gattatcagg gggtgctgct gaccgacctg ctggcggaag tgggcgggct
      241 gactgaagac ctgggggtga atattgtagc cgaagatggc tacatgatga ctttctctta
      301 taaccagctc acgctgggcg atttcatcac ctatgacccg gcgacggggg cggagaagcg
      361 cgttgaaggt ccgctgcagg tgctggtggc atatgcacgg ggtggtgagc tgctggatcc
      421 caagggagat ggggtgctgc ggctggccat catcagcccg aaaaacgacc aggtgacgga
      481 tgggcactgg agcgtgaagt gggtgcgcaa gatcgagctg aagccggtgg gggacgagtg
      541 gaacctggcg atgaagggag cggtggaggg cgccatcgac cggaattcat tccagtcgtg
      601 ctctgcgccg ggctgccacc aggtaaactg gaccgacggc gagaaccagg tgtggacggg
      661 agttccgctg tggacgctgg tgggctgggc ggatgatttg atccagcact ccggaccggc
      721 gtattttgat gccctggcag atgccgggta tacgatcgag atcatcgcgg ccgatggata
      781 ttccatcaat ctggacagtg tgcgggtgaa acggaataac gagctgctgg tggcgtacca
      841 ggtcaacggg gcgcctttgc cagataaata ttttcccctg cgcctggtag gggcaggtgt
      901 gtcagacaac gcagaaagca tcggggcgat cagcgagatc gacctgatca tcgacccggc
      961 agtggcggcc tctgtgccgc ctctgctgcc ggtgggtgaa gcagtgatgg cagctctggc
     1021 agagccagcc atcgcaggcg atttgaatat tggtgggctg gtgaacaatc ccctgggatt
     1081 gatggaagaa gatctgcgcg gcgtgagccc ggttaagatc actgtggagc atcccaagca
     1141 ggggccaaca gagtacgaag gcgtttattt gaacctgctt ttgaccctgg cgggagttca
     1201 gggagggggt acgcagctgg tgctgacggc aggggatggg tacacgacca caatcgactt
     1261 gctgcaggcc cgcgcctgca cggactgcct ggtagctttc accgaggcgg cgggtgaatt
     1321 gactctggtg atgccgggct ttgacagctc ggcctgggtg aaggacatca cccacatcga
     1381 agtgaagtag ccgccaccct cttcggcggg ggtgggtgac cggacagatc accggtgacc
     1441 ggtaagacca ccggtgaccg gacagatcac cggtgaccgg acagatcacc ggtgaccgga
     1501 tacaggagcc tgacgctccg agtctgaagg aaccgagcac cttcaggcaa tgcgcggaag
     1561 agcccatgtg gaaccggacc tccgacaccc acccctgatc ttttttcagc tggatgaaaa
     1621 gatggcagcg gacatcccag cttgtcccag gtattttatg ttcgcgggct tctattcttg
     1681 ccaactctgc gccattggcc ggggcgcctg aattgaacca cttcccgtta tgaagcgaca
     1741 atgcaaacga aaaaagtcat tcaaagaatc ctccaggcag cgatttcagg ctgcctgatt
     1801 gcgagctgca gctctgcaac cgcttaccct ccgcaggaaa cagcaagccg agcaccaaca
     1861 accgctgagg ggcagctggc tacagagaac cagccgatga agcctggcgc cgagacgcta
     1921 gagctgggcg agactccaac agcgcccagc ccaactcctg tggtgcagcg catggaatct
     1981 ggtgtgctgc gggtgttctg cgccgggagc ctgatcctgc cgttcagcga gctggagaaa
     2041 gccttcgaag ccgccaatcc ggagatcgat gtgcagaccg agttccacgg cagtatccag
     2101 gtgatgcggc atgtgaccga cctgcacgag gcgattgatg tggtggcaac tgccgaccgg
     2161 gcattgatcc cgatgctgat gtatgccaca gacgaccctg acacgggaga accgtatgcg
     2221 gactggtacc tgcattttgc cggaaataag gtgggactgg cctatacgac tgagagccgc
     2281 cgggcagggg agatcaatgc ccagaactgg tacagcattc tggccgaccc gggggtgcgc
     2341 tttgggctgg ctgacccgcg catcgatccc tccggttacc gcacactgat ggcgctgcgc
     2401 ctggcggaag tatattatgg gcagccgggc atctttcagg atctgatggg cagcaaattc
     2461 agttatccga tcgggatatt taaagaagcc ggctacactg agatcagcgt gccagagatc
     2521 ctggagccaa agctggacag cagcctggca ctgcgggggg cgagtatcca gctggtagcg
     2581 ctgctgcagt cgggcgacct ggactatgcc ttcgaatatg agagcgtgat ccgccagcac
     2641 gggctggagc tgctggagct gccggaggcg gtgaacctgg gcagcgcggc gcaggcggag
     2701 acttaccggc aggtggaggt gaagctggat ttccagagat tcgctaaaat ccagccggta
     2761 ttccgcggcg agccaatcgg ctacgggatc acgattccga gcaatgcacc ggagcctggt
     2821 ctggcggagc agtatatcca gttcttactc tcggaagagg ggcgggggat tatggaagcg
     2881 tattatcacc cggtgtttga gcaggtgggc tgtgatggag tggagaatat gccggaggtg
     2941 ctgagaggtg tatgcctgga gtgacggata aatggaatct cgtccagcag attgcttcac
     3001 cgaagagcgt ggcccggatt gcttcgccaa agagagtggc tcgcaatgac tgataaaaat
     3061 ggaattgttc cagtatttgt cgccctgggc agcctgctgg
//
LOCUS       DATMXK010000398         3184 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_92117, whole genome shotgun sequence.
ACCESSION   DATMXK010000398 DATMXK010000000
VERSION     DATMXK010000398.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3184)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3184)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3184
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_92117"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1109)
                     /locus_tag="VN363_07410"
     CDS             complement(<1..1109)
                     /locus_tag="VN363_07410"
                     /EC_number="2.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012382.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008483 - transaminase activity [Evidence
                     IEA];
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal phosphate-dependent aminotransferase"
                     /protein_id="HWQ84376.1"
                     /translation="MDFAERVHNLAPEGAYHMLARAQALEAAGRKIIHLEIGQPDFPT
                     FENIVDAGIQSIRDGFTRYTPTAGIKPLREAIALQVSQRKGLPVKPGQVVVGPGAKPA
                     LFFPTLALVRPGDEVIYPDPGFPTYPAMIEVAGGTPVPVPLREAEDFSFDLEAFDRSI
                     SPRTRLAILNSPSNPTGGVMPVSALQHIAAAAQAHNFWVLSDEIYDQLVYEAPAPSIA
                     ALPGMADRTVICDGFSKAYAMTGWRLGFGIMPEALAERVELLLTHSIGCTASFTQVAG
                     LEALQGPQEEVHRVVAEYRRRRDVLVAGLNRIPGITCRMPQGAFYAFPNITGLGKSSA
                     WLADYLLAEAGVAVLPGTSFGANGEGYLRLCFANS"
     gene            1307..2185
                     /locus_tag="VN363_07415"
     CDS             1307..2185
                     /locus_tag="VN363_07415"
                     /EC_number="2.5.1.15"
                     /inference="COORDINATES: protein motif:HMM:NF005719.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydropteroate synthase"
                     /protein_id="HWQ84377.1"
                     /translation="MNTTLTNRSKIVLIGPDQPFVMIGERINPTRRKKLAETMAQGDY
                     SVALEDARRQIEAGGQVLDVNAGIPGGDEPALLRGVTEALLAETDTPLCFDSANPEAL
                     AAALSIYPGKALINSTTAEASMMEKVFPLAKQYGAAVIGVITDESGIPATAEDRLAVA
                     AKLVRRAGDYGIPPEDILIDCLALTVGADHKAGQVTLDAMALVQKELGVNLSLGASNV
                     SYGLPDRKIINTAYLALAVGRGMTAAITDPTVPEIRTTLLACDLLMGRDEYSMHWIKA
                     YRKRLKAAEAQAKAQQ"
     gene            2209..>3184
                     /locus_tag="VN363_07420"
     CDS             2209..>3184
                     /locus_tag="VN363_07420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84378.1"
                     /translation="MLSVSGKARVADFLGDRMVYRNLNPADGRLAGFETTRRLLGLPS
                     GYAPRKSELDYARVVYELLQQAQHLQAPGRALRQIVFIGDTRRNDGLTFDNLCQVSGL
                     PGTAFIGDETTTPVNIEVAQTAGGRSLYLANRWEALLDPGEQGFRKFCQRQGLVADEG
                     TVLLVDLDKTALAARGRNARVIDQVRIRAVENTVAESLGASFDPHAFRTAYDRLNQPE
                     FHSFTADNQDYLAYICLILGSGIVDLDDLVGQVLQGSWSRFADFIEQIEARAGELGGG
                     LADLHTEIYTRFLQGDPTPFKAFRRKEYLATIHRMGCLGDDAPLLERME"
BASE COUNT          697 a          861 c         1024 g          602 t
ORIGIN      
        1 atggaattcg caaagcacag gcgcagataa ccctctccat tggctccgaa ggaggtgccg
       61 ggcagcaccg caactcctgc ttctgcaagc aagtaatcgg ccagccaggc ggagctttta
      121 cccagaccgg tgatgttggg gaaagcataa aacgcgccct gcggcatccg gcaggtgatc
      181 ccggggatcc gattcagacc cgcgaccagc acatcccggc ggcggcggta ttctgccacc
      241 acgcggtgga cttcttcctg gggaccctgc agggcttcaa gaccggcaac ctgggtgaag
      301 ctggcggtgc agccaatgga gtgggtcagc aggagctcga cgcgttcagc cagggcttcc
      361 ggcatgatcc caaagcccag ccgccagccg gtcatggcat aagctttgga gaagccgtcg
      421 caaatcacgg tgcgatcagc catgcccggc aaagcggcga tgcttggggc gggagcctca
      481 taaacgagct ggtcgtaaat ctcatccgag agcacccaga agttatgcgc ctgagcggcg
      541 gcggcgatgt gctgcaaggc ggacactggc atcaccccac cggtaggatt gctgggggaa
      601 ttaaggattg ccagccgggt gcgtggactg atggaccggt cgaacgcttc caggtcaaag
      661 gagaaatcct ctgcttcgcg cagcggaacc ggcacaggcg tgccgcccgc gacctcgatc
      721 atggcgggat aggtcgggaa gccggggtcg ggataaatta cttcatcccc cgggcgtacc
      781 agtgccaggg tagggaagaa caacgccggc ttggcgccag gaccgacaac tacctggccg
      841 ggtttcacgg gcagtccttt gcgctggctg acctgaaggg cgatggcttc gcgcaagggt
      901 tttatgcccg cagtaggggt ataacgggtg aaaccatccc ggatcgactg gataccggca
      961 tcgacgatgt tttcaaaggt gggaaaatct ggctgcccga tttccaggtg gatgattttc
     1021 ctcccggcag cctccagggc ttgagcgcgg gcgagcatgt ggtaggctcc ctcgggggcc
     1081 agattgtgga cgcgttcggc aaagtccatg ctgtgttcct ctcgactggc tgctggtggt
     1141 tgatgaaccg gtcagatttt ttgattaagc caatcatatc accgggtaac gcaccagggc
     1201 agaggctcac tccacaccgg gatgcggctg gctcgtggac aaagactatc cggcgtgtta
     1261 taaatggcaa gcagctaccc aaattaaagc aaaaccaagg aaaaaaatga atacgacttt
     1321 aaccaaccgt tcgaaaatag tcctgattgg cccggaccag ccattcgtga tgatcgggga
     1381 gcgaatcaac cctacccggc ggaagaaatt ggccgagaca atggcgcagg gcgactattc
     1441 ggtcgctctg gaagatgccc gccgccagat cgaggctggc ggccaggtac tggacgttaa
     1501 tgccggtatt cccggcgggg atgaaccggc cctgctgcgc ggggtgactg aggcgctcct
     1561 ggcggagacc gatactccgc tgtgttttga ttcggcaaac ccggaagccc tggcagcggc
     1621 tttaagcatt taccccggca aagcgctgat caactctacg accgctgagg ccagcatgat
     1681 ggaaaaagtc tttcccctgg cgaaacagta tggtgccgcg gtcatcgggg tgattacaga
     1741 tgaatcgggc atccctgcga cggcggagga ccgcctggcg gtggcagcca aactggtccg
     1801 gagggcaggc gattatggta ttcctccgga agatatcctg attgactgtc tggcgctcac
     1861 cgtgggcgcg gatcataaag ctgggcaggt gaccctcgac gcgatggcat tggtgcaaaa
     1921 agagttgggc gtaaacctga gcctgggagc cagtaatgtc tcgtacggtc tgccagatcg
     1981 caagattatc aatacggcct acctggcgct ggcggtaggg cgcggcatga cggctgccat
     2041 aaccgatccg acggtgccgg agattcgtac gaccctgctg gcctgtgacc tgctgatggg
     2101 acgggacgag tattccatgc actggatcaa ggcgtaccgc aaacgtttga aagcagctga
     2161 agcgcaggcg aaagcccaac agtaatggta agttagaagg caagcgcaat gctgagtgtg
     2221 tctggcaaag ccagggtggc ggattttctg ggggaccgga tggtgtatcg caatctgaat
     2281 ccagccgatg ggcggctggc aggatttgag accacccgcc gcctgctggg cctgccatcc
     2341 ggatatgcac cgcgtaaaag cgagctggat tacgcccggg ttgtctacga gcttctgcag
     2401 caagcccaac acctgcaagc gccgggaagg gctttgcgcc agatcgtctt tatcggggat
     2461 acgcggcgca atgatgggct gacctttgat aacctgtgcc aggtaagcgg tttaccgggg
     2521 acggcgttta tcggggatga aaccactact ccggtgaaca tagaggtcgc ccaaaccgct
     2581 ggagggcgct cgctctatct ggcgaaccgg tgggaagccc tgctggaccc tggtgagcaa
     2641 ggattcagga agttttgcca gcgccagggg cttgtggcag acgaaggaac agtcctgctg
     2701 gtcgacctgg ataagacggc tctggcagcg cgcgggcgca atgcccgggt gatcgatcag
     2761 gtgcggatcc gggcagtaga gaatacggta gctgaaagcc tgggtgccag cttcgatcca
     2821 cacgccttcc ggacagcata tgaccgcctg aaccagccgg aattccacag ctttaccgcc
     2881 gataatcagg attatcttgc ttatatctgc ctgatcctgg gcagtgggat agtcgacctg
     2941 gatgaccttg tcggacaggt cctccagggg agctggagca gatttgctga tttcatcgag
     3001 cagatcgaag cccgcgctgg agagctggga ggcggattgg ctgacctgca tacggaaatc
     3061 tatacccgtt ttctgcaagg ggatccgact ccattcaagg cattccggcg gaaagaatac
     3121 ctggcaacga tccaccggat gggctgcctg ggagatgatg cgccgctttt ggagcgaatg
     3181 gaaa
//
LOCUS       DATMXK010000399         2200 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_160896, whole genome shotgun sequence.
ACCESSION   DATMXK010000399 DATMXK010000000
VERSION     DATMXK010000399.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2200)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2200)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2200
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160896"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(183..1004)
                     /locus_tag="VN363_07425"
     CDS             complement(183..1004)
                     /locus_tag="VN363_07425"
                     /inference="COORDINATES: protein motif:HMM:NF042459.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6573 family protein"
                     /protein_id="HWQ84379.1"
                     /translation="MLDAAVAAAMLQHEMSALEQEHLRVILLDTRNRVIDIVEIYHGS
                     LNSAQVRVAELFKPAIQRMSAAIILVHNHPSGDPTPSPDDVAVTRAVVQAGKLLDIDT
                     LDHLVIGRGRFVSLKERGLGFDGRVSEMKLNYRLRSVRYSIHEDDDLIYAYTRSHALE
                     DGVLVDVSQMASEAGFRYPTAITADLHARITLNEREKALGQSYEGRLWDVVFLASFVA
                     RRSGTQDRCGLDVNLFEADDAPPHRTHRRTLSLWMVVGPGDQGEPVITIGFPEDF"
     gene            1176..1904
                     /locus_tag="VN363_07430"
     CDS             1176..1904
                     /locus_tag="VN363_07430"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ84380.1"
                     /translation="MESAYILLVDDERGITDYLAPILERAGYSVTVARDGQTALQKVH
                     ELHPDLIVLDVLMPGIDGREVCRQIRAGGNWTPVIMLTRVSTLTDKVLSLEEGADDYL
                     CKPFEPQELIARIRAVLRRRGSGKPGKPLSMAGKLRSGDLVLDRKARRLEKGGQVIAL
                     TPKALALLEHLMLHPDQVFSREQLMDAVWGWAQPVASRAVDVRIAELRHAIGDDENTA
                     YIETVTGIGYRFLAPVESAEEEAR"
     gene            1901..>2200
                     /locus_tag="VN363_07435"
     CDS             1901..>2200
                     /locus_tag="VN363_07435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84381.1"
                     /translation="MKLRRGPVIALILSPLTVGLILAVALQSPGVANPVLRFQTRADL
                     GTLLLVASGILTASVFGFWLSRRRTNARLRIAAEKVKEEAKGERRRFLRQLDHELK"
BASE COUNT          470 a          602 c          663 g          465 t
ORIGIN      
        1 ggccggggac atcgtggatg tacggcaggg gtaaaccttg acctgtatct gactttgagc
       61 cggagacaag gatgtgaccc tgcttctgcc ggttggctgc tttgaggacg cgttcccgga
      121 agtaggtgtt ctcaaagggg gcaggcaggc ctgggattga ttttccagtc atggctgcct
      181 ccctaaaaat cctcagggaa accgatggtg ataaccggtt cgccctgatc tccgggacca
      241 accaccatcc acaaggaaag tgtacggcgg tgggtgcgat gcggaggcgc atcgtcagct
      301 tcgaacagat tgacatccaa gccgcatcga tcttgtgtac ctgatcggcg agcgacgaag
      361 gatgccagga aaaccacatc ccatagccgg ccttcgtagc tctgacctag cgctttctcc
      421 cgctcattca gggtgatgcg ggcgtgcaag tcggcggtga tggcggttgg atagcgaaaa
      481 ccggcttccg atgccatttg agagacatct accagcacgc catcttccag cgcatgggag
      541 cgtgtatagg cgtagatcaa atcgtcatct tcatggatgg aatatctaac agatcgaagg
      601 cgatagttaa gtttcatctc agaaacgcga ccgtcaaaac cgaggccgcg ctctttgagc
      661 gaaacgaagc gaccacgacc gatgaccaga tggtccagcg tgtcgatatc caagagcttg
      721 cccgcctgga caactgcccg ggttactgca acatcgtctg ggctgggcgt cgggtcgccg
      781 gacggatgat tatgcaccag gatgatcgcc gccgacatgc gctggatggc aggcttgaac
      841 agctctgcca cccgcacctg ggctgagttc aggctaccgt ggtagatctc cacgatgtcg
      901 atgacccggt tgcgcgtgtc cagcaggatc acccgcaggt gctcctgttc cagtgcgctc
      961 atctcgtgct gcagcatagc agccgcaacg gctgcgtcca gcatgaaagg ccggtcatta
     1021 gcgattcgtc cagacaggca atgaagaaag attatgtttt tatgtggtca agggttactt
     1081 ttatcacttg cgaaattccc tgaagccccc tatagtgaaa aagtcggaag gaattcatcg
     1141 attctcctga gcatgctgga gaataaggtt tgtttatgga aagcgcttat atccttttgg
     1201 tggacgacga gagggggatt accgactacc tggcgcccat cctggagcgc gctggctatt
     1261 ccgtaactgt ggctcgtgac ggccagactg ccctacagaa agtccatgaa ttacaccccg
     1321 atttgatcgt tttggatgtc ctgatgccgg gaatagatgg tcgcgaagtg tgccgccaga
     1381 tacgtgcggg cggcaactgg acgccggtga tcatgctcac tcgcgtgagc accctgacgg
     1441 ataaagtcct gagcctggaa gaaggagccg atgactattt gtgcaagcct ttcgagcccc
     1501 aggaactgat cgcccgcatc cgggccgttc tccggcgcag gggaagcgga aagccgggaa
     1561 agccgctcag catggccgga aagctccgct cgggcgatct ggtgctcgat cgcaaagccc
     1621 ggcgtctcga gaaaggcggg caggtcatcg cgctgacacc gaaggctctg gcgttgcttg
     1681 agcacctgat gctccatcct gatcaggtat tcagccggga gcaattgatg gacgcggtgt
     1741 ggggctgggc gcaacccgtg gcttcccggg cggtggatgt gcgcatcgcc gagctgcgcc
     1801 acgctatcgg cgacgatgag aataccgcat acattgaaac tgtgactgga attggctatc
     1861 ggttcctggc tcctgtggag agcgcggaag aagaggcgag atgaaattgc gccgtggtcc
     1921 cgtgattgcc ctgatcctct ctcctctgac ggttggcctg atccttgccg ttgccctgca
     1981 atctccgggg gtcgccaacc ctgtcctacg cttccagaca cgcgccgacc tggggacgct
     2041 gctcctcgtc gccagcggca ttctcactgc cagcgtcttt ggcttctggt tatcccgacg
     2101 cagaacaaat gctcgtcttc gaatagctgc cgaaaaggtg aaggaagaag ccaaaggaga
     2161 gcgacgccgt ttcctgcgcc agttggatca cgaattgaag
//
LOCUS       DATMXK010000400         2434 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_15084, whole genome shotgun sequence.
ACCESSION   DATMXK010000400 DATMXK010000000
VERSION     DATMXK010000400.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2434)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2434)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2434
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15084"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1588)
                     /gene="ligA"
                     /locus_tag="VN363_07440"
     CDS             complement(<1..1588)
                     /gene="ligA"
                     /locus_tag="VN363_07440"
                     /EC_number="6.5.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559957.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003911 - DNA ligase (NAD+) activity
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD-dependent DNA ligase LigA"
                     /protein_id="HWQ84382.1"
                     /translation="MTTDEELQKRLAELRQQLTFHNYRYHVLDDPVISDVEFDQLLVE
                     LRQIEADHPEWVTPDSPSQRAGAPMLDKFVKVQHPAPILSLANAFGTPDLRAWYERVR
                     RLDDRVERAHFVVEPKIDGLSVVLHYKDGVLIQGATRGNGEVGEEITANLRTVRSLPL
                     RIPANPQGPVPPAYFVVRGEVYMPIKEFEQLNKTLEANGQKTYLNPRNTAAGSLRQLD
                     PGLTATRPLNLLIYQIVAADGEIPHTQMGLLQYLQELGFPTPPAEYCTDLEEAVRVVE
                     SWEERRHQLPYEADGVVIKIDDLELQADLGFAGKDPRGAIAYKFPALEVTTLLKDIRV
                     NVGRTGKLVPNAVLEPVMIGGVVVKQATLHNFDYIRDKDIRIGDRVRVKRAGEVIPYV
                     IGPVEAARTGAEQPYFPPQACPVCGEPAEHLPGEVDWFCVNASCPEQLVRNLEHFVSR
                     GAMDIVGLGIRIVEQLAQSGLVKDVADIYSLRRDDLLGLEGFAEKKADNLLEAIETSK
                     GRPLSRVITALGIRGVGEVVA"
     gene            complement(1603..>2434)
                     /locus_tag="VN363_07445"
     CDS             complement(1603..>2434)
                     /locus_tag="VN363_07445"
                     /inference="COORDINATES: protein motif:HMM:NF014665.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA];
                     GO_function: GO:0016653 - oxidoreductase activity, acting
                     on NAD(P)H, heme protein as acceptor [Evidence IEA];
                     GO_process: GO:0006784 - heme A biosynthetic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="COX15/CtaA family protein"
                     /protein_id="HWQ84383.1"
                     /translation="CNGVVMPRAAEIETLIEFTHRLTSGAALLLVLGLLVWFWRSFPK
                     GHPARLGAWLTAIFILLEAMVGAMLVLNEWVAGDVSIGRVVSQGIHLLNTFLLLASLT
                     LTVYWAATGHPIRLKGQGWLGAGLGIGVVLILILGVSGAITALGDTLFRVDTLAEGLA
                     QDINPDSHFLVRLRVWHPVIAVVTGLYTIFIALLTSLFFPHKGNKRLAIALVVLFVVQ
                     LGAGLLNLVLLAPVWLQIVHLLLADLVWVTYVFMAAALLAAPAEQSQPAFVDKMAVSV
                     "
BASE COUNT          502 a          803 c          682 g          447 t
ORIGIN      
        1 cagccaccac ttcacccacc ccgcggatcc ccagcgctgt gatcacccgg ctcagcggcc
       61 ggcccttaga cgtttcgatc gcttccagca ggttgtcggc tttcttttca gcaaagccct
      121 ccagccccag cagatcgtcc cgccgcaggc tgtatatatc cgcaacatcc ttcaccagcc
      181 cggattgggc gagctgctcc acgatccgga ttcccaggcc gacgatatcc atcgcacccc
      241 gggagacaaa atgctccagg ttgcgcacca gctgctccgg gcaggaggcg ttcacgcaga
      301 accagtctac ctcgccgggc aggtgctcgg ccggctctcc acacaccggg caggcctgcg
      361 gtggaaaata gggctgctct gctccggtcc gggcagcctc caccgggccg atcacatacg
      421 ggatcacctc gccggcgcgc ttgacccgca cccggtcacc gatgcggata tctttgtcgc
      481 ggatgtaatc aaaattatgc agggttgcct gcttcaccac caccccgccg atcattaccg
      541 gttccagaac cgcattcgga accagcttcc ccgttctgcc tacattgacc cggatatctt
      601 tcaagagggt ggtcacctcc agggcgggaa acttgtaagc aatcgcgccg cgcgggtctt
      661 taccggcgaa gcccaggtcg gcctgcagct ccaggtcatc gatcttgatc accaccccat
      721 ccgcctcgta cggcagctgg tggcggcgct cctcccagga ttccacaacc cgcacggctt
      781 cttccagatc ggtacagtat tcggccggcg gggtggggaa gcccagctcc tgcaggtact
      841 ggagcagccc catctgggta tggggtattt cgccatccgc ggcgacgatc tggtagatca
      901 gcaaattcag ggggcgtgtg gcggttaaac cggggtcgag ctggcgcagc gagccggcag
      961 cagtattgcg cggattgagg taggtttttt gcccgttggc ttccagggtt ttattcagct
     1021 gctcgaattc tttaatcggc atatatactt caccccgcac cacaaaataa gccggaggta
     1081 ccggtccctg cgggtttgcc gggatgcgca gcggaagcga ccgcacggtg cgcaggttgg
     1141 cagtgatttc ctcgcccact tcgccgttac cgcgcgtggc gccctggatc aaaaccccat
     1201 ccttgtaatg cagcaccacc gaaagcccat cgatcttcgg ctccaccaca aaatgggccc
     1261 gctccacccg gtcatccagg cggcggacac gctcgtacca ggcgcgcaga tccggtgtgc
     1321 cgaaggcatt ggccaggctc aggatcgggg caggatgctg gactttgacg aatttatcca
     1381 gcatcggcgc cccggcgcgc tgggacgggg aatcgggcgt cacccactcc gggtgatcgg
     1441 cttcgatctg gcgcagctcg accagcagct ggtcaaattc aacgtcactg atcaccggat
     1501 cgtccagcac atggtagcgg taattgtgaa aggtaagctg ctggcgcagc tctgccaggc
     1561 gcttctggag ttcttcatca gtggtcattc ccatctccct gattacactg aaacagccat
     1621 cttgtccacg aaagccggtt gggattgttc ggcaggcgcc gccaacaatg ctgctgccat
     1681 aaatacatac gtcacccaga ccaggtctgc caggagtaaa tgcacaatct gcagccacac
     1741 cggcgccagc aggaccaggt tgagcagtcc ggcgcccagc tgcaccacga acaacacgac
     1801 cagggcaatc gccagccgtt tattcccttt gtgggggaag aacaggctgg tcaataaggc
     1861 gataaagatc gtgtacagcc cggtgaccac ggctatgacc ggatgccaga cccgcaggcg
     1921 caccaggaaa tgcgagtcgg gattgatatc ctgggccaat ccctccgcca gggtatccac
     1981 ccgaaaaaga gtatctccca gggcagtgat cgccccactc acccccagga tcaggatcaa
     2041 gacgacccca atacccaggc cggctcccag ccagccctgc cctttcaggc ggattggatg
     2101 gccggtcgca gcccaatata cggtcagggt aaggctggcg agcagcagga aggtattcaa
     2161 tagatggatt ccctgcgaga ccaccctccc gatggataca tctcctgcca cccactcgtt
     2221 gagcaccagc atcgccccca ccattgcttc gagcaaaatg aagatcgccg tcagccaggc
     2281 gcccaaccgg gccggatgcc ctttggggaa agagcgccag aaccagacta acagccccag
     2341 aaccagcagc aaagccgccc cgctggtcag gcggtgggtg aactcgatca gcgtttcgat
     2401 ttccgctgcc cggggcatga cgaccccatt gcac
//
LOCUS       DATMXK010000401         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_36555, whole genome shotgun sequence.
ACCESSION   DATMXK010000401 DATMXK010000000
VERSION     DATMXK010000401.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36555"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..378
                     /locus_tag="VN363_07450"
     CDS             <1..378
                     /locus_tag="VN363_07450"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ84384.1"
                     /translation="PMNRFTHFEFVTSDFEKTAQFYRTVFGWKVEKWEGPVDYWTITT
                     GDETTVGINGGLMPAGGPFSGTVNTIEVDNIDEYIAKVEANGGEFIFPKDVIPGGGYI
                     AYFKDTTGVICGLHQADEKAGMV"
     gene            705..1178
                     /locus_tag="VN363_07455"
     CDS             705..1178
                     /locus_tag="VN363_07455"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWQ84385.1"
                     /translation="MNIPAILTSQYLASLEMLKQVILQCPEPVWNAPEDANKFWHTAY
                     HALYYIDKYLQYPPQGITPWSKHRPGWEDFPAPPDGEFHDKATLLEYLAYCQQQVKEK
                     LPSMDLTAAEAHGPLTMLELQIYSIRHIMQHVGELLERLGSRTGAQIDWVGSVRD"
     gene            1197..1769
                     /locus_tag="VN363_07460"
     CDS             1197..1769
                     /locus_tag="VN363_07460"
                     /inference="COORDINATES: protein motif:HMM:NF013989.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="dihydrofolate reductase family protein"
                     /protein_id="HWQ84386.1"
                     /translation="MQSKIYYAFNVSLDGFIEDARGSLDWSNPDEELHRHFNQKEAET
                     DIFLYGRRLYETMAAFWPTAEQDPSLPEYILEYARIWNAKPKLVFSKTLAEVGSNSRL
                     VRDDLAGEIRKLKAQPGKTISVGGAGLAASLMQLGLIDEYSVYIHPVILGGGKPMFPA
                     LRQKIDLQLVGTQTFSSGVVLLRYEIVNER"
BASE COUNT          514 a          609 c          570 g          494 t
ORIGIN      
        1 cccatgaacc gtttcacgca tttcgaattt gtgaccagcg attttgagaa aaccgctcag
       61 ttttaccgca ccgtcttcgg ctggaaggtc gaaaagtggg agggtccggt ggattattgg
      121 accatcacca ccggcgacga gactaccgtt gggatcaacg gcggcctgat gccggccggc
      181 ggccccttca gcggcacggt caacaccatc gaagttgata atatcgatga atacattgcg
      241 aaggttgagg ctaacggcgg cgaattcatc ttccccaagg atgtgatccc gggggggggc
      301 tatattgctt atttcaagga taccaccggc gtgatctgcg gcctgcacca ggccgacgaa
      361 aaagccggga tggtatagtt tctttaccta gaccctgata aattttaaaa ttcctcttcg
      421 ctaaccattc attgcagacg actgtacagg tttccgcatg ccgcatcagt atccggatta
      481 ccgtccagcg tctgacatgg atcgtttcta atatcattaa aacctgtccg gtctggacat
      541 gcgtagcgta gcagacctta caggttttaa tgttttctga gcaggtcgag atgatatcct
      601 tcctcagaca taatcggtca agttatgccg aaacctgtag ggtctattac cgatctggta
      661 ggtaaagact gcttagggtc cgatactcaa aaaggagaac gataatgaac atccctgcca
      721 tcctcacctc gcagtacctg gcctcgctcg aaatgctcaa gcaggtcatc ctccaatgcc
      781 cggaaccggt ctggaatgcg cccgaggatg ccaataaatt ctggcacacc gcttaccacg
      841 ccctgtacta tatcgataag tacctgcaat atccacccca ggggatcacc ccctggagca
      901 agcaccgccc cggctgggaa gatttccccg ccccgccgga tggtgaattt cacgacaagg
      961 ccaccctcct ggaatacctg gcttactgcc agcaacaggt caaggaaaag ctacccagta
     1021 tggacctgac tgccgcggag gcgcacggac ctctgacgat gctcgagctg cagatctatt
     1081 ccatccgcca catcatgcag catgtgggcg agctgctgga gcggctcggt agccgcaccg
     1141 gcgcccagat cgattgggtc ggatcggtgc gtgattgagg ggaggggagt gaaatcatgc
     1201 aaagcaagat ctattacgcc ttcaacgttt ctctggatgg ctttatcgag gatgcccggg
     1261 gcagcctgga ttggtcgaat cccgatgagg agctgcaccg gcactttaac cagaaggagg
     1321 ctgaaacgga catcttcctg tatgggcggc ggctgtacga gaccatggcc gccttctggc
     1381 ccactgctga gcaggaccca tccttgcccg aatatatcct tgaatatgcc cgcatctgga
     1441 acgccaagcc caagctggtt ttctcaaaaa ccctggctga agttgggtcg aactcccggc
     1501 tggtccggga cgatctcgcc ggagagatcc ggaagctgaa agcccaaccc ggcaagacaa
     1561 tcagcgtggg cggggccggg ctggctgcca gcctgatgca gctgggcctg atcgatgaat
     1621 acagcgtcta catccacccg gtcattctcg gcggcggcaa gcctatgttt cctgccctgc
     1681 gccagaagat cgacctgcag ctggtcggga cgcagacctt ctcgtccggc gtcgtcctgc
     1741 tcagatacga gattgttaac gagcgctgac acctcgcgac cctgataaaa tttaacatgc
     1801 cataccctaa tcgattttta cattcttctt cggtaacctt catttacaga cgaccgtaca
     1861 ggtttccaca tgccgcctgg gtatccagat gaccatccag cgccagccta ccgccgtttc
     1921 agattgcctt aaaacctgta cggtctggcc atggtgcgag gaacagaccc tacaggtttt
     1981 aaggttgtct tagcaggtcg ggattatatc cttccagaca aacatgatcg gaaaagttat
     2041 acggaaacct gtagggtcca ttaccgatct ggattgtaaa aactgatcag gttttaatat
     2101 tttctgaatt gatccgggtt acacccgggc acaaagcccc aatcggtaag gttcttcgga
     2161 aacctgtagg gtccattacc gatctgg
//
LOCUS       DATMXK010000402         2034 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_94387, whole genome shotgun sequence.
ACCESSION   DATMXK010000402 DATMXK010000000
VERSION     DATMXK010000402.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2034)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2034)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2034
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94387"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(167..400)
                     /locus_tag="VN363_07465"
     CDS             complement(167..400)
                     /locus_tag="VN363_07465"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84387.1"
                     /translation="MSSRITCPMCGFEYDPASQVVCQGCPLQRGCGLVRCPNCGFETP
                     DLQHSKLAGWAARLFKEPILIPGRQKLSGKAKL"
     gene            complement(397..>2034)
                     /locus_tag="VN363_07470"
     CDS             complement(397..>2034)
                     /locus_tag="VN363_07470"
                     /inference="COORDINATES: protein motif:HMM:NF019292.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transporter B"
                     /protein_id="HWQ84388.1"
                     /translation="GYPMLLALNMMDEAGKRGLNIDTDGLANTLGFPVVPTVAVQRDG
                     LTTLPQALNTARPANFHIQYSPEIEQTVDQVIDICAAAWLEQSSISPRSLALMFLTRD
                     RVAEEWLASKLPPEDYHRLEGTRAAAQSHSLELLGMQIQQTRIARAGELSAAALVDSG
                     VQGNRLSNLLGQLTMHPLWGLPVLALVLLVVYYFVGIFGAGTLVGFLENNLFGEIINP
                     WLVEIVERISPFQWLSDILVGKYGLWTMGVTYAFALILPIVTTFFFAFGILEDSGYLP
                     RLAALSNRMFRLLGLNGKAVLPMVLGLGCVTMATLTTRVLETKRDRLLVTLLLALAVP
                     CSAQLGVVMGLLAGVSMGAALLWSGVVVLVLMLVGWLAARLVPGERSQLLVELPPLRI
                     PQLSNVVLKTLARLEWYVKEVVPLFLLGAGLMYLLDLSGILAILIRWGEPLVAGWLGL
                     PAETSAAFIMGFIRRDFGATGLFLMSNQGLLTPVQIVVAMVTITLFIPCIASVMMISK
                     ERGWKTALAMFAIIMPLAFVTGGLLNRFLLWTGWGLG"
BASE COUNT          480 a          623 c          598 g          333 t
ORIGIN      
        1 aactgagtct ggcgctctgc ggcaagcccg ggccggaaac caatcacttt tgccaggctg
       61 ccggcgggaa tctccgccaa tgtttttgcc gggcttgaac ttgagttatc taccgatcga
      121 aggtaacccg atgggtgaca agcgctgaaa taagccggaa atatattcat aattttgcct
      181 taccactaag cttttgacga cctgggatca atattggttc cttgaaaagc cgggcagccc
      241 agccagccag cttcgagtgc tgtaaatcgg gcgtctcaaa gccgcagttc gggcagcgga
      301 ccagtccaca gccgcgctgc agcgggcagc cctggcagac aacctggctg gcggggtcat
      361 actcaaaccc acacatcggg caggtgatcc ggctgctcat cccagccccc agccagtcca
      421 cagcaagaag cggttcagca gcccgccggt aacgaacgcc agcggcatga tgatcgcaaa
      481 catcgccagc gccgtcttcc agccgcgttc cttggagatc atcatcacgg aagcgatgca
      541 ggggataaat aaggtgatcg tcaccatggc aaccacaatc tggaccggcg tgagcaggcc
      601 ctgattactc atcaaaaaca aaccggtggc gccgaaatcg cggcggatga agcccatgat
      661 aaaggctgcg ctggtctcgg cgggcaggcc cagccagcca gcaaccagcg gctcgcccca
      721 gcggatcagg atggcgagga taccactcag atcgagcagg tacatcaggc cggctcccag
      781 caggaagagc gggaccacct ctttgacata ccattccagg cgcgccaggg tcttcagcac
      841 gacgttcgat aactgcggga tgcgcagggg cggcagctca accagtagct ggctgcgctc
      901 gccgggaacc agccgggctg ccagccagcc gaccagcatc aaaaccagga cgaccacccc
      961 gctccacagc agagccgcgc ccatggagac gccggccagc agccccatga caacgccgag
     1021 ctgggctgaa cagggcactg ccagcgccag cagcagggta accagcaggc ggtcgcgctt
     1081 agtttcaagc acgcgggtgg tcagggtcgc catggtcacg caccccaggc ccagcaccat
     1141 cggcagaacg gctttcccgt tcaaacccag cagcctgaac atgcgattcg agagggctgc
     1201 caggcgcggc aaatagccag aatcttcaag gatcccgaaa gcgaagaaga aggtagtcac
     1261 gattggcagg atcagggcga atgcataggt aacacccatg gtccacagcc cgtacttgcc
     1321 caccaggatg tccgaaagcc actggaaggg gctgatcctc tcgacaatct caaccagcca
     1381 ggggttgatg atctcgccga agagattatt ttcgagaaaa ccgaccagtg tgccagcccc
     1441 gaaaattccc acaaaataat agaccaccaa taagaccagc gccaggaccg gcagccccca
     1501 gagcgggtgc atggtcagct ggccgaggag attggagagg cggtttccct gcacgccaga
     1561 atcgaccagg gcagcggcgc tgagctcgcc ggcccgggca atgcgggtct gctggatctg
     1621 catccccagc agctccaggc tgtgggattg ggcggcggcg cgggttcctt ccagccggtg
     1681 ataatcttca gggggaagct tgcttgccag ccactcctca gccacgcgat cgcgggtcag
     1741 gaacatcaac gccagcgaac ggggtgaaat cgaagactgc tccagccagg ctgccgcaca
     1801 gatatcaatg acctggtcca cagtttgctc gatttctgga gaatactgga tgtgaaaatt
     1861 tgccgggcgg gcggtattta atgcctgcgg gagggtggtg agaccatctc tctgcactgc
     1921 gacagtgggc actaccggaa aacccagcgt attcgccagg ccgtcagtgt caatattcag
     1981 gccgcgcttg ccggcttcat ccatcatatt gagcgccagc agcattggat aacc
//
LOCUS       DATMXK010000403         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_45235, whole genome shotgun sequence.
ACCESSION   DATMXK010000403 DATMXK010000000
VERSION     DATMXK010000403.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_45235"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..1098)
                     /locus_tag="VN363_07475"
     CDS             complement(61..1098)
                     /locus_tag="VN363_07475"
                     /inference="COORDINATES: protein motif:HMM:NF025030.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HEAT repeat domain-containing protein"
                     /protein_id="HWQ84389.1"
                     /translation="MTVVFTLPKDQRTNRPLLESCLEDDPAAVTRLLKLLGHPTPELR
                     HAYQDAIRQANKTRLWRGLISILADRCWETSQDQPQPIQQLHPDLEHQLRIDEGILDL
                     LASDHTPAESPQKTAALRDCARSHSVQQRYIAHYLLVSRGDYTALDNLKSLFPQASLE
                     WQLRMVRLLANLNTTEGGPLLIEALSSSNRILHHAAHRALIEQGKYATDAWITALQHP
                     DSHIRWHAARGLGEMGDLRAIAILAAGLRDENAAVRWATSGLLARMGSRAVPAILHEI
                     SRKPLDEPARQAAYHALHGMSGHDLSARLAPILDALHGPGSDLLAPSTAQKLLARWEE
                     LPESAAQIDQT"
     gene            complement(1320..2372)
                     /locus_tag="VN363_07480"
     CDS             complement(1320..2372)
                     /locus_tag="VN363_07480"
                     /inference="COORDINATES: protein motif:HMM:NF013341.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="RIO1 family regulatory kinase/ATPase"
                     /protein_id="HWQ84390.1"
                     /translation="MPSKNEFDEINTELADIPFQRIRNAGPAHSKAGLRNRKPAQTIQ
                     DSPLPLTSDGEDDSLEAALLTYNPSRHERVWILDSLSDFYDQQWFGDILRQVKGGKEA
                     SVYQCVAKTAIQAPFLAAKVYRPRRFRNLKKDHLYREGREYLDENGNAIRNHGMLHAI
                     QKKTAWGKELTHTAWIEYEYRTLQILHQAGADVPVPYTRGNNAILMSYIGGEDQPAPT
                     LQEVHLSRAEARQVYHRALYNVELMLDHNLIHGDLSAFNILYWQEQITLIDFPQVVDP
                     DQNVNAYWIFERDITRLCEYFSPYGIKSNAPRLAASLWRNRGWRIEHPVDPAYLDADR
                     PEDRRYWNHENSGAQH"
     gene            2710..2955
                     /locus_tag="VN363_07485"
     CDS             2710..2955
                     /locus_tag="VN363_07485"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84391.1"
                     /translation="MLKRLEQGRMLVERLEKLSPDSRWARRASGARGNLVKLLEELQD
                     MLDLQLAGAAEDWQRLDQLVRFGFWILEKAAHELVAG"
BASE COUNT          613 a          763 c          930 g          652 t
ORIGIN      
        1 catcgcacca gtacacatcg cggtcgttag ccataattgc ctcctttttt taaaatttac
       61 ctacgtctga tctatctggg cagcagactc tggcagttct tcccagcgag ccagaagctt
      121 ctgggcggta ctcggcgcca gcagatctga gcccgggcca tgcagcgcat ccaggattgg
      181 cgccagccga gcagataaat cgtgcccgga catgccgtgc agggcgtggt aagctgcctg
      241 gcgagcaggc tcgtccaggg gtttgcggct gatttcatgc aggatggctg gtacggcgcg
      301 gctgcccatt cgcgccagta agccggaggt cgcccagcgc acggcggcat tctcgtctcg
      361 caacccggca gccaggatgg cgatggcgcg caggtcgccc atctctccca atccccgggc
      421 ggcatgccag cggatgtggc tgtcaggatg ctgcagggca gtgatccagg catcggtggc
      481 atattttccc tgctcgatca aggcgcggtg ggctgcgtgg tgcaggatgc gattgctgga
      541 gcttaaagcc tcgatcaaga gcggtccacc ctcagtcgtg ttcaggtttg ccagcaagcg
      601 caccatccgc agctgccact cgagactggc ctgtgggaac agggatttca ggttatccag
      661 cgcggtgtaa tcaccgcggc tgacgagcag ataatgggcg atatagcgct gctggaccga
      721 atgggacctg gcgcaatcgc gcaatgcggc ggttttttgg ggagattctg ccggggtatg
      781 gtcgctggca aggagatcca ggatcccttc gtcgattcgc aattggtgtt ccagatcggg
      841 atggagctgc tggatcggct ggggctggtc ctggctggtc tcccagcagc gatcggcaag
      901 gatactgatc aggccgcgcc acagccgggt tttattcgcc tgacggatgg catcctggta
      961 ggcatggcgc agctctggcg tcggatggcc gagtaatttt aacagccggg tgacagcagc
     1021 cgggtcgtcc tccaggcagc tctcgagcag cgggcggttg gtccgctgat ccttgggcag
     1081 ggtaaagaca acagtcatcg cacaccttcc ttcaaaaggt acggcccggc ggatggcgag
     1141 aatgccagcc ggccgggccg agatgaaggg cggaaagctg ccgtatgcag gttgggtacc
     1201 cggtcaggat cagaaccgct gggcgtctag cgttctgaaa aaggcattcc tccacgtaaa
     1261 caggatagga ttgaatccat cacacctata ctatagcaaa tatttgctgg taatgcaaat
     1321 tagtgttgtg cgccactgtt ctcatggttc cagtagcggc ggtcctccgg gcggtcagca
     1381 tccaggtaag ccggatctac cgggtgttcg atgcgccagc cgcggttgcg ccacaggctg
     1441 gcagccaggc ggggggcgtt agacttgatc ccgtaaggag agaaatattc gcatagccga
     1501 gtaatatcac gttcgaagat ccagtaggca ttcacgtttt gatccggatc gactacctgt
     1561 ggaaagtcga tcagggtgat ttgctcctgc cagtagagga tgttgaaggc cgataggtcg
     1621 ccgtggatca ggttatggtc gagcatcagc tccacattgt acagggcgcg atgataaacc
     1681 tggcgagcct cagcccggct gaggtggacc tcctgcaggg tcggggcagg ctggtcttca
     1741 ccaccgatat agctcatcag gatggcattg ttgccccggg tataagggac cggtacatcc
     1801 gctccagcct ggtggaggat ctgcaaggtg cggtattcat attcgatcca ggcggtatgg
     1861 gtgagctcct tgccccaggc ggtctttttc tggatggcgt ggagcatccc atggttgcgg
     1921 atcgcattgc cgttttcatc cagatattca cgcccttcac ggtagagatg gtccttcttc
     1981 aggttgcgaa agcggcgtgg gcggtagact ttggctgcca gaaagggagc ctgaatggcg
     2041 gtcttcgcca cacactggta caccgaggct tccttgccac ctttgacctg gcgcaagata
     2101 tcaccgaacc actgctggtc atagaaatca cttaaagagt cgagaatcca gacccgctcg
     2161 tggcgagaag gattataggt cagcagggcg gcttccaggc tgtcgtcctc cccatcgctg
     2221 gtgagtggaa ggggcgaatc ctggatggtc tgggccggtt tcctattcct caacccggct
     2281 ttggagtggg ccggaccggc attacggatg cgctggaagg gaatatctgc cagctcagtg
     2341 ttgatttcat caaattcatt cttcgaaggc atcgaaatct acctcaataa ggattagagc
     2401 tgtctataat cgagtgtggc atgaaaaagc cacagataat tcgcgggtct gtggcataaa
     2461 aaaccacagg cggagtgccg cctgtggata agggttcgag attgcgggaa ataccaggat
     2521 ggtttatccg atcattacac aggcgcacaa aacagaatgt cttccgacaa cagtgctggc
     2581 tgtcatttgt gcgcctcctt cttgagagat tccagaattt tgcttacaat gagaatagtt
     2641 tacacggcaa tttgaaaccc gtcaagacta atattgtccg attgggggtg cgttttggaa
     2701 cactctgaga tgttgaaacg gcttgagcag ggtcgcatgc tggtggagcg gctcgaaaag
     2761 ctttcccccg attcacgctg ggcgcggcgg gccagcggtg caaggggaaa tctggtcaag
     2821 ctattagagg agctgcagga catgctggac ctgcagctgg caggcgcggc agaagactgg
     2881 cagcggcttg accagctggt gcggtttggt ttctggattt tggagaaagc tgcccacgag
     2941 ctcgtggctg gttaagcg
//
LOCUS       DATMXK010000404         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_50074, whole genome shotgun sequence.
ACCESSION   DATMXK010000404 DATMXK010000000
VERSION     DATMXK010000404.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50074"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(157..1158)
                     /locus_tag="VN363_07490"
     CDS             complement(157..1158)
                     /locus_tag="VN363_07490"
                     /EC_number="2.1.3.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_005318430.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004585 - ornithine carbamoyltransferase
                     activity [Evidence IEA];
                     GO_function: GO:0016597 - amino acid binding [Evidence
                     IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006591 - ornithine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWQ84392.1"
                     /translation="MSYNLRNRSFVKLLDFTPQEIRFLLALARDLKAAKYGGYEQPRL
                     KGKNIALIFEKASTRTRCAFEVAAYDQGAHVTYLGPEGSQIGHKESMKDTARVLGRMY
                     DGIEYRGFAQKTVETLAQYAGVPVWNGLTDEFHPTQILADILTMSEHSNKPLEEISYA
                     YLGDARNNMGNSLMVGGCKLGMDVRMCAPKALWPKEELVQACREIAAETGARLTLTEN
                     VAEGVRGADFLHTDVWVSMGEPKEVWDERVKLLKPYQVNRQAMELTSNPQVKFMHCLP
                     SFHNRDTRVGEDIYQKTGLDGLEVTEEVFESAASIVFDQAENRMHTIKAVMVATLGD"
     gene            complement(1226..>2059)
                     /locus_tag="VN363_07495"
     CDS             complement(1226..>2059)
                     /locus_tag="VN363_07495"
                     /EC_number="3.4.13.-"
                     /inference="COORDINATES: protein motif:HMM:TIGR01887.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_function: GO:0016805 - dipeptidase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Sapep family Mn(2+)-dependent dipeptidase"
                     /protein_id="HWQ84393.1"
                     /translation="TLGFTPDSRFPLIYAEKGVLQVTLEGTNETGMRISGGNAYNAVP
                     DQAVYEGLQQAELVQELDRLGYGYVRDGDSVRVLGTAAHAQVPEEGVNAVTRLAQALL
                     GIGETAPALEFIVKELGADPFGRGMFGDCQDELTGRLKCNVGMLELGDQARLSLDLRI
                     PVSVEKDWVVSRLQPAGERYGFSYQEREWLAPLYMPLDHFLIQTLLQVYREVTGDYES
                     QPFTAGGATYARAMPNCVAFGAVFPDRPKVEHQPNEHIRLDDLFQAMQIYAQALYALT
                     R"
BASE COUNT          419 a          631 c          539 g          470 t
ORIGIN      
        1 agcttatcct ggccggagtg agagcctggt tgggcctgta atctgccagc tcgaggctgc
       61 caccgggcta aagattgctg tacgttgaga cagcgccacc tgagaactcg cagccctccc
      121 atgaacctag cgcagaatgg aataaaaaat ttccggtcag tcgccgagtg ttgcgaccat
      181 cacagcttta attgtgtgca tccggttttc agcctgatca aagacgatcg atgcggccga
      241 ctcgaatact tcttcagtaa cttccaaccc atccaggccg gttttctgat agatatcttc
      301 gccgaccctg gtgtcccggt tgtgaaacga cggcaggcag tgcataaatt taacctgggg
      361 attgctggtt agctccatcg cctggcgatt cacctggtac ggcttgagca gcttgacccg
      421 ctcatcccag acctctttcg gctcgcccat ggatacccac acatcggtat gcaggaaatc
      481 cgccccgcgc acaccttccg ccacgttctc ggtcagagtc aggcgcgccc cggtctctgc
      541 tgcgatctcc cggcaagcct ggaccagctc ttctttaggc cacagtgcct tcggagcgca
      601 catccgcaca tccattccca gcttgcatcc acccaccatt agcgaattgc ccatgttgtt
      661 gcgcgcgtcc cccaggtagg catagctgat ctcttccaga ggcttattgc tgtgttcact
      721 catggtcagg atatccgcca ggatttgagt gggatggaat tcatcggtca ggccgttcca
      781 caccggcacg ccggcgtact gcgccagggt ctcgacggtt ttttgagcaa agccccggta
      841 ttcgatccca tcatacattc gccccaggac ccgggctgta tctttcatcg attctttatg
      901 cccgatttgt gagccttccg gtcccagata ggtgacatgg gcgccctggt cgtaggctgc
      961 tacttcaaat gcacagcggg tgcgggtcga ggctttttca aagattaacg cgatattttt
     1021 gcctttcagg cgcggctgct cgtacccgcc gtatttcgcc gctttcaaat cacgcgcaag
     1081 cgctaacaag aagcggattt cctgaggggt gaaatcaagt aatttgacga agctccggtt
     1141 gcgcaggtta tacgacatag aattacttcc tttcaggggt cagatggttt ttcgaatcga
     1201 gtgcattcag tcgggcgtat ccgggtcagc gggtaagcgc atacagcgcc tgggcataga
     1261 tctgcatggc ctggaacaga tcatccaggc ggatatgctc gttcggttgg tgctccactt
     1321 ttggccggtc ggggaaaacc gccccaaaag cgacacaatt cggcatggcg cgggcgtagg
     1381 tggcgcctcc ggctgtgaaa ggctggctct cataatcgcc agtgacctcc cggtaaacct
     1441 gcagcagggt ctggatcagg aaatgatcca gcggcatata caacggtgcc agccattccc
     1501 gctcctggta gctgaacccg tagcgctccc ccgccggttg taagcgcgat accacccaat
     1561 ccttctcgac cgagaccggg atgcgcaagt ccaggctgag tctggcttga tcgccaagct
     1621 ccaacatgcc gacattgcat ttcaatcttc cggtaagctc gtcctggcag tccccaaaca
     1681 tacctctacc aaagggatct gcgccgagct ccttgacgat aaattccaga gccggcgctg
     1741 tctccccaat cccaagcaag gcttgcgcca ggcgggtgac cgcgttcaca ccttcttctg
     1801 ggacctgggc atgggcggct gtgccgagga cgcgcacact gtccccatct cgcacatatc
     1861 catagcccag tcgatcgagt tcctgcacca gctctgcctg ctgcagcccc tcataaacag
     1921 cctggtcagg caccgcatta taggcattcc caccgctgat ccgcattcca gtttcattcg
     1981 tcccttccag ggtcacctgc aggacgcctt tttcagcata aatcaacgga aagcgcgagt
     2041 ccggtgtgaa gcccagggt
//
LOCUS       DATMXK010000405         7398 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_11793, whole genome shotgun sequence.
ACCESSION   DATMXK010000405 DATMXK010000000
VERSION     DATMXK010000405.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7398)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7398)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7398
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_11793"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1548
                     /locus_tag="VN363_07500"
     CDS             <1..1548
                     /locus_tag="VN363_07500"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84394.1"
                     /translation="MPVYSKRAYFMVVALIFLAASLLPYIFAAGAAGDELVFGGFLFN
                     PIDGNTYLAKMQQGYSGTWLFTLPYTAEKGAGWLLFIYYLFLGHLARWSGLSLLVIYH
                     LARLAGCIVLLAGLFRYIQRSLGSGAPARLAFLLAALGSGMGWLALPSGAYTADFWVA
                     EAYPFLSGFATPHFSLGLGLLLFLIAPLAEPAPALPAKQSAWKALRTFLLAFCLAIIS
                     PFGVVIAGVALAWPAWKAFFPFRRDSLAALLGERLPWVLLGGLPWLIYDQAAAWRDPL
                     LAGWDAQNLTPTPAVWDLLVAFSPLIFLAIPGAVQALRRGPEPLRQAAAWLLLGILLL
                     YAPLGLQRRFMIGLYVPVVVLGTAFLAARIPDSRRLWLAGSVILALVLPTNLIIHLST
                     WQVVAAGDPNLYYTRGEAQAFTWIASQTEPESLILAAPDTGLLLPAHTGRRVIYGHPF
                     ETVNAEVEKALVEAYFATPDPAVLAERGVDYVFYGPRERRLGSTAPQGLPVVYESGGV
                     TLYAAAP"
     gene            1532..3715
                     /locus_tag="VN363_07505"
     CDS             1532..3715
                     /locus_tag="VN363_07505"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84395.1"
                     /translation="MLPPPEPSRRTSWLPYLLLLFPLALILPGLGDFAYPPGEALFSD
                     IPISHFPAAVMLKNLIQEYRQLPLWSPHLFSGAPLAANPLYSLYYPPAWLALILPLPA
                     GFNLLVILHLMWGGLGMLRLLQAEGLRWQAAVLGALSFIALPKLYAHYGAGHLTLLYA
                     VPWTPWLLLAWKMTGTASYSRRWRVPAGAILGVIFLADPRWAAYAGLLWLGYAVTRWV
                     GLPGWGARRQDLGQLLIHLLLAALIAAPLALPLLEFTRLTTRAHLTPADNLEFSLPLA
                     RLLGLLIPSLGGTHEYELYSGALVLLLAVLACFWGAVRKRASFWLAAAVLSLLWALGE
                     NLPGLSGLASLPGFDLLRVPSRSLFITGMAISALAAYAVDAFLAETTPPKRLGLVLAS
                     LAFFVLLLAFGSWAISGQTSLNLVWGALLLVVSAIWAGLRLRGRLTPRPWWVGCLVLI
                     LIDLGIADQKAFAFHPFEQVLGEKRALVEYLESQPGLFRVYSPSYSLPQQVTAAAGLE
                     HAGGVDPLQIQAYASFFARAAGTPLSGYSISLPPFASADPAVDNRSAVPDASQLGLLN
                     VAYVAAEYPLAAPGLEWVGEFTGTQLYQNTRVLPRAWLQPGDSPLGESIQPVSSLEWS
                     PNRIVVRLDPPPGQSLQLVFSEIAYPGWRAWIDGQPAALEAAGGLLRSVMVPGGAHEV
                     VLKYRPLSVVIGLLLGILGVVFTVIAGRLSRKQTGPRSFDQVTAP"
     gene            3712..6051
                     /locus_tag="VN363_07510"
     CDS             3712..6051
                     /locus_tag="VN363_07510"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ84396.1"
                     /translation="MSRPPLYRSVIIISIASGFVASLGYLTWLDPPAVSLLPEALLFL
                     VLVIVTTLLGVPLGGGTASLLPMAVIASYFVLGLIPAAWIALLGEIIAEALEAFATSG
                     GAQPRPKNRLETVTRLAANATLQPASILVAGGLYQAVGGRQPFVAITPDLIIPLIILG
                     LTYLLVDYLGAGLFFAGRGRDWLESFLTELPRMLTYEGLPLVFAPWVALVSGRMGTGA
                     LAILVVGLVAVAWTARSLAYARQRLERRVRELGVLQEVGRALGSNLNLEELLQAVYTQ
                     VASLMPAGYFYISLFDPALDEVSFPFVIEAGQRTTFPTRRSGTGLTELILRTGQPLLS
                     RAPTSETRQMLGLSPAARPAASFLGVPLIVASRTVGVIALQSFDARNLYDEGHQNVLT
                     SVAAAAAIAIQNARLFARLDQDLNLRLHQLDSVLKAARDGMLFLDCQGQVVMANRTAA
                     DYLGLAQLGVYGADGDPASEAQAQFLAGRLGYCPGELDQALADLLEGKVDIQRQTLSL
                     PLLPDLAIERTLLPVRDQAGSVIGWLFVLHDRTQEVRLAALQDDLVEMLVHDLRSPLT
                     IIQGGLEVIAQSASEDIHSPASLVLLPMQSSTRRMLHLVNEMLEIQRMENGELVVQPT
                     RLPVRAFLEDCASPYTAQLVEAGISLAIDISPDLPDLLVDTAHTERLLHNLLDNAIKF
                     TPDHGRISMAARLDSIQHDRAVLITLQDTGSGIPAADQQSLFQKYRQIEGSSGRRHGS
                     GLGLYYCRLVAEAHGGKIWVESLPERGTCFHILLPAAQP"
     gene            6054..>7398
                     /locus_tag="VN363_07515"
     CDS             6054..>7398
                     /locus_tag="VN363_07515"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84397.1"
                     /translation="MPASKCQPRFTLAIWLAPFMLLAPVILSGKALFWGTPALQFIPW
                     QAYAWDTLLHGELPLWNPWLGMGAPLVANYQSALFYPPNWLGLAAYAAGGVAALAWIQ
                     APLAALHLAWAGWGMAKLARSLGLGPLPQTVAGLAFALSGYLVARLHFLSITFTVAWL
                     PWVLLGAVALVQPRRDWQAYLRLAASLGMMLLAGHAQLSWYTVLLAGLLAGWLAWHTP
                     AAETGWQIRLKVVGNVWLKLAGAGILALALSAIQLLPTAEYLLQSQRAGAVSFETGLD
                     YSYWPWHFLTLLAPGLFGSPAQGTYWGFANYWEDAVYIGLLPLLLGLGVLLKRNSTTA
                     SPGQRAVIVFFRWLVPVTILLALGENTPVFPWLYRNIPTFDMFNAPARWIIYLILGLA
                     LLAGFGVQRWRRPTGRGLYWTRLATMGAFAVSLGAGIGWLLLKDVKATFLGAAALT"
BASE COUNT         1081 a         2281 c         2303 g         1733 t
ORIGIN      
        1 atgccagttt attcaaaacg tgcgtatttt atggtcgtcg cgttaatatt cctggcggcc
       61 agccttctac cgtatatctt cgctgccggt gcggccggag acgagctggt ctttgggggc
      121 tttttattca atcccattga cggcaacacc tacctggcca agatgcagca gggatattcc
      181 ggcacctggc tgttcaccct gccgtacaca gccgaaaaag gcgcgggctg gctgctcttc
      241 atttattacc ttttcctggg gcatctggcg cgctggagcg ggctgtccct gctggtgatc
      301 taccatctgg cgcgcctggc aggctgtatt gtgctcctgg cagggctgtt tcgctacatc
      361 cagcgcagcc tgggtagcgg tgcgccggcg cggctggctt ttcttctggc ggctttgggc
      421 tcaggcatgg gctggctggc cctgccttct ggcgcctaca cggccgattt ttgggttgcc
      481 gaggcttatc ctttcttgtc cggctttgcc actccccatt tttcgctcgg gttgggtttg
      541 cttttgtttt tgattgcgcc tctggccgag ccagctcccg ccttgccagc gaaacaaagt
      601 gcctggaagg cgctgagaac atttctgctg gctttttgcc tggcgatcat ctcgcctttt
      661 ggcgtggtga tcgcgggcgt ggctctggcc tggccggcgt ggaaagcgtt cttcccattc
      721 cgccgggata gcctggcagc cctgctgggt gaacgcctgc cctgggtttt gctcggaggc
      781 ctgccctggc tgatctatga ccaggctgca gcctggcgcg atcccttgct ggctggctgg
      841 gatgcccaaa acctgactcc gacaccagct gtatgggatc tgctggtcgc tttttcacca
      901 ttaatcttcc ttgccattcc tggggcagtc caggctctgc gccgaggtcc agagccactt
      961 cgccaggctg ccgcctggct gctgttgggg atcctgctgc tgtatgcacc actcggcctg
     1021 cagcgccgct tcatgattgg gctgtacgta ccggtcgttg tcctgggtac agccttcctg
     1081 gcagcgcgca tcccggactc ccggcgttta tggctggctg ggagcgtgat cctggcgctg
     1141 gtcctaccga ccaatctgat catccacctg tccacctggc aggttgtggc ggcgggtgat
     1201 ccaaacctgt attacacccg gggagaggcg caggctttta cctggatcgc ctcgcagact
     1261 gagccagaga gcctgatcct ggcggcgccc gataccggcc tgctgctgcc tgcccacacc
     1321 gggcggcgcg tgatctacgg gcatcctttc gagacggtga atgctgaggt ggaaaaggcg
     1381 ctggtcgaag cgtatttcgc caccccggac ccggctgtgc tggcagagcg cggggtggat
     1441 tatgtctttt acggcccacg tgagcgccgc ttgggcagca ccgcaccgca ggggctgccg
     1501 gtggtctatg agagcggggg agtgaccctg tatgctgccg ccccctgagc catctcgccg
     1561 gacctcctgg cttccctatc tcctgctgct gtttcccctg gcattgatcc tgcccggtct
     1621 gggtgatttc gcctaccctc ccggggaggc gctcttctcc gatattccca tctcccattt
     1681 tcctgcggcc gttatgctga aaaacctgat ccaggaatat cgccagctgc cgctctggtc
     1741 gccgcacctg ttcagcgggg cgccgctggc tgccaaccca ctgtacagcc tgtattatcc
     1801 cccggcctgg ctggcattga tcctgccgct cccggccggt ttcaatctgc tggtgatcct
     1861 gcacctgatg tggggtggat tggggatgct gcgcctcctg caggcggaag gcttgcggtg
     1921 gcaggcggca gtcctgggtg cactgagctt tatcgccctg cccaagctgt atgcgcatta
     1981 tggggccggg cacctgaccc tgctgtatgc ggtaccctgg acaccctggc tgctcctggc
     2041 ctggaaaatg acagggacag cgagttacag ccgccgctgg cgtgtgccgg ctggtgcgat
     2101 cctgggggtg atctttttgg ccgacccgcg ctgggcggcg tacgcaggcc tgctctggct
     2161 ggggtatgcc gtaacccggt gggttggctt accaggttgg ggagcgcgcc ggcaggatct
     2221 gggccagctg ttgatccacc tccttttggc agctttgatc gccgctccac tggcgctgcc
     2281 gttgctggaa tttacccgtc tcaccacccg cgcccatttg acccccgcgg ataacctgga
     2341 attttcgctg cccctggcgc gcctgctggg gctgctgatc ccttcgctgg gcggtacgca
     2401 cgagtacgag ctctattccg gtgcactggt tctgctcctg gctgtcctgg cgtgtttctg
     2461 gggcgccgtc cgcaaacggg cgagcttctg gctcgcggcg gcagtgcttt ccctgctctg
     2521 ggcgctgggc gagaacctgc ccgggttaag tgggctggcc tccttacccg gtttcgatct
     2581 gctgcgcgtg ccttcccggt cgcttttcat tactggaatg gcaatttcgg cgctggcagc
     2641 ctatgctgtg gatgcattcc tcgctgaaac cacgcctccg aaacgcctgg gtctggtgct
     2701 ggcaagcctg gctttttttg ttttgctcct ggcgtttgga agctgggcga tcagcgggca
     2761 aacgagcctc aatctggtct ggggcgcgct gctgcttgtg gtgagcgcga tctgggcagg
     2821 gctgcgcctg cgcggcaggc ttaccccacg accctggtgg gtgggctgcc tggtcttgat
     2881 cctgatcgac ctgggcattg cggatcaaaa ggcattcgct ttccatcctt tcgaacaggt
     2941 gctgggagaa aaaagggctc tggtggaata tctggaatcg cagccgggtc tcttccgggt
     3001 gtattccccg tcgtacagcc tccctcagca ggttactgct gcagccgggt tggagcatgc
     3061 cggcggagtc gacccgctcc agatccaggc gtatgccagt ttctttgccc gggctgctgg
     3121 cacaccgctt tccgggtaca gtatcagcct gccgcctttc gccagcgccg acccggcagt
     3181 ggataatcgt tcggctgtgc cggatgccag ccagctcgga ttgctgaatg tggcttatgt
     3241 ggccgcggaa tatccgctgg ctgcccctgg tttagaatgg gttggcgagt tcactggtac
     3301 ccagctttac cagaataccc gggtgttacc gcgggcctgg cttcagccgg gtgattctcc
     3361 cctcggcgag tcaatccaac ccgtctccag cctggaatgg tcgccaaacc ggattgtggt
     3421 ccggctcgat ccaccgcctg gccaatccct ccagctggtg ttttcggaaa tcgcctaccc
     3481 cggctggcgg gcctggattg atggccaacc ggcagccctg gaagccgccg gaggcttgct
     3541 gcgctcggtg atggtgccgg gcggagctca tgaagtcgtc ctgaagtacc gcccgctcag
     3601 cgtggttatt ggcctgctat tgggcattct gggggtcgtc tttactgtga tcgctggcag
     3661 gctttcccga aagcagaccg ggccgcgttc cttcgatcag gtgactgcgc catgagccgt
     3721 cctcccctgt acaggtccgt gatcattatc agtattgcct ctggattcgt agccagcctg
     3781 gggtacctga cctggctgga tcctcccgcg gtgtcgcttt tgccggaagc cctgttattc
     3841 ctggtcctgg ttatcgtcac cactctgctg ggggtgccgc tgggtggtgg gaccgcctca
     3901 ctcctgccca tggcagtcat cgccagctat tttgtgcttg gcctgatccc ggctgcctgg
     3961 atcgccctgc tgggagagat catcgctgaa gccctggagg cattcgccac cagtgggggc
     4021 gcacagccgc gccccaaaaa tcgcctggag acggtaacca ggctggcagc caacgccacc
     4081 ctccagccag cttcgatctt ggtcgcgggc ggtttatacc aggcggtggg cgggcggcag
     4141 ccgttcgtgg caattacacc ggatctgatc attcccctga tcattctcgg cttgacctac
     4201 ctgctggttg attatctggg tgcggggctg ttcttcgccg gccgcggtcg cgattggctg
     4261 gaatcttttt tgaccgagct gcccaggatg ctgacctacg aaggcctgcc tctggttttt
     4321 gcaccctggg tcgctctggt ctccggacgg atgggaactg gcgctctcgc catcctggta
     4381 gtaggcctgg tggcagtcgc ctggactgcg cgcagcctgg cttacgcccg ccagcggctc
     4441 gaacgccggg tgcgcgagct gggtgtgctg caggaagtag ggcgtgccct gggatccaac
     4501 ctgaatctgg aagagctgct gcaggctgtc tacacccagg tcgccagcct gatgccagcc
     4561 ggttattttt atatctcgct gtttgatccc gccctggatg aggtctcttt tcctttcgta
     4621 atcgaagccg ggcagcgcac aactttccct actcgccgca gtgggactgg tctgaccgag
     4681 ctgatcctgc gtaccgggca gcccttgctc agccgcgctc ccacctccga gacgcgccag
     4741 atgctgggtc tttcgccagc agctcgcccg gcggcttcct ttttaggtgt gccattgatc
     4801 gtcgccagcc ggacggtggg agtgatcgcc ctgcagtcct ttgatgcccg caatctatat
     4861 gatgaaggtc atcaaaatgt gctgacctct gtggctgctg ccgccgcaat cgccatccaa
     4921 aatgcgcgcc tgttcgcccg ccttgaccag gacctgaacc tgcgtctcca ccagctcgat
     4981 tcggttttga aagctgcccg ggatggcatg ctgtttctgg attgtcaggg tcaggtggtg
     5041 atggccaatc gcaccgctgc ggactatctc ggtctggctc agctgggagt atatggcgcg
     5101 gacggcgatc cggcatccga agcgcaggcg cagttcctgg ccggacgttt gggttattgt
     5161 cccggcgaat tagaccaggc gctggcggat ctactggagg gcaaggtaga tatccagcgc
     5221 cagaccttga gcctgccgct cctgccggat ctggcgattg agcgcaccct gctcccggtt
     5281 cgggatcaag ccgggagcgt gatcggctgg ttgttcgtct tgcacgatcg cacccaggag
     5341 gtccgcctgg cagctttgca ggatgacctg gttgaaatgc tggtccacga tttacgatct
     5401 cctctgacta ttatccaggg cggtttggaa gtaatcgccc agtctgcctc cgaggatatt
     5461 cactctccag cctctctcgt actgctgccc atgcagagca gcacccggcg gatgctccac
     5521 ctggtgaatg aaatgctgga aatccagcgt atggaaaacg gagagctggt tgtgcagccc
     5581 acgcgcttgc cagtgcgggc gttcctggaa gattgcgcca gcccgtatac tgcccagctg
     5641 gttgaagccg ggatcagcct ggcaatcgat atatccccag atttacccga cctgctggta
     5701 gacacagccc atacagagcg gctgctccac aacctgctgg acaatgccat caaatttacc
     5761 cctgaccatg gcaggatcag tatggctgcc aggttggatt caatccagca tgatcgggct
     5821 gtgctgatta ccttgcagga taccggatca gggattcctg ctgccgacca gcaatccttg
     5881 ttccagaagt atcgtcagat agagggcagc tccggtcgaa ggcacggcag cggcctgggt
     5941 ttatattact gccggctggt cgctgaagca cacgggggta aaatttgggt ggagagcctg
     6001 cctgaacgcg gcacatgttt tcatattttg ctgccggcag cgcagccata accatgccag
     6061 cctccaaatg ccagccccgt ttcacgttag cgatctggct ggcgccattc atgctcctgg
     6121 cgccagtaat attgagcggc aaagccctgt tctggggaac cccggcgctg cagttcatcc
     6181 cctggcaagc ctatgcctgg gacaccctcc tgcatggcga gcttccactc tggaatccct
     6241 ggctggggat gggcgcgccg ctggttgcca actaccagtc agccttgttt tacccgccca
     6301 actggctggg gctggcggcc tatgccgccg gcggcgtggc cgctctcgcc tggattcagg
     6361 cacccctggc agctctgcac ctggcctggg ccggttgggg tatggcgaag ctggcccgca
     6421 gcctggggtt ggggccgctg cctcagacgg tcgccggtct ggcctttgcc ttgagcggct
     6481 atctcgtagc ccggctgcat ttcttgagta ttaccttcac cgtcgcctgg ctgccctggg
     6541 tgctgctggg tgcagtagcc ctggttcagc cgcgccgcga ttggcaggct tacctgcggc
     6601 tggcagccag cctagggatg atgctgctcg ccgggcatgc ccagctttcc tggtacaccg
     6661 tcctgctggc cggcttgctg gcaggctggc tggcctggca tacccctgcg gcggagactg
     6721 gctggcagat ccgtttgaaa gtggttggaa atgtctggtt gaagctggct ggggcaggga
     6781 tccttgccct ggcgctcagc gcgatccagc tgctgcctac cgccgagtac ctgctccaat
     6841 cccagcgcgc cggggcggtg agcttcgaga ccggcctgga ttattcctac tggccctggc
     6901 attttctgac cttgctggcg ccggggttgt ttggcagccc ggcgcagggt acttactggg
     6961 gatttgccaa ttactgggaa gatgcggtct atatcggcct gctgcctttg ctgctcgggc
     7021 tgggcgtcct gctcaagcgt aactcaacca cggcttcccc aggccagcgc gcagtgatcg
     7081 tcttcttccg ctggctggtg ccggtgacga tcctgctcgc cctgggtgaa aatacgccgg
     7141 tcttcccctg gttgtaccgg aatattccca cctttgatat gttcaatgcc ccggcgcgct
     7201 ggatcatcta cctgatattg ggcctggcgc tgctggctgg cttcggcgtt cagcgctggc
     7261 ggcggccaac cgggcgcggc ctgtactgga cccgactggc gacgatgggg gctttcgcag
     7321 tctctctggg agccggaatc ggctggctgc tcctgaagga cgtgaaagca acgtttctgg
     7381 gtgctgccgc cctgacag
//
LOCUS       DATMXK010000406         5122 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_117874, whole genome shotgun sequence.
ACCESSION   DATMXK010000406 DATMXK010000000
VERSION     DATMXK010000406.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5122)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5122)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5122
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117874"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1310)
                     /locus_tag="VN363_07520"
     CDS             complement(<1..1310)
                     /locus_tag="VN363_07520"
                     /inference="COORDINATES: protein motif:HMM:NF012428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartate aminotransferase family protein"
                     /protein_id="HWQ84398.1"
                     /translation="MADPEQIEKLLNGYRAEIMARSPRSLAIWQQNRTVMPAGVGSLF
                     RLSDPFPMVVKSARGARLWDADGNEYLDYMLGFSTMLLGNAPEEVETALREALPRGTS
                     YGQCHEHEYEFAQLFCEMVPGVEKVTFCNSGTEATLYALRLARAATGRPLVAKFEGGY
                     HGAHDLLAVSYGRPGLDMDAYGPIEDPTAIPESPGLAAGAWQDTIVLPFNHPAAFEKI
                     RRHADRLAAVIIEPVQGAAGTIPADPDFLAELRRLTQQTGALLIFDEVITGFRLAPGG
                     AKEYYGVIPDLSTYGKVAGGGLPFGAIGGTAAAMRLLEYDLEPQRAILMAGTFNGNPL
                     VTAAGTAVLRRLQGDPDLYLRINALGDCFRDEINRFAREQDFPALATGVGSMFWMHAA
                     PGPIQSVRDAWQADPAASAGLRLLFRKNGLHISPRHGFISAAHTP"
     gene            complement(1342..2154)
                     /locus_tag="VN363_07525"
     CDS             complement(1342..2154)
                     /locus_tag="VN363_07525"
                     /inference="COORDINATES: protein motif:HMM:NF012424.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016746 - acyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2-oxo acid dehydrogenase subunit E2"
                     /protein_id="HWQ84399.1"
                     /translation="MIDNLGPTHVVELTPGRRAWLNTLDLSVPSHWMYGLLEVDVTLP
                     RQLIADHKARTGEALSFTGFLVYCLAQAVAEHKDLQAYRKGHSQLMLFDDVNVGLMVE
                     HKAGEKSALMGLIVQAANRKTYPEIHAEIRTAQSAPVPPNRGMPAWFRSILLLPWPLS
                     RLVKAWLNLSTRRNPAVRVAAGGTVCVTAVGMFGKDHSGWGIAATPHSLGLVVGSIAW
                     KPAVVAGRIEPREILNLTVLFDHDIVDGAPAARFTNRLVELIECGEGLLAAG"
     gene            complement(2151..3773)
                     /locus_tag="VN363_07530"
     CDS             complement(2151..3773)
                     /locus_tag="VN363_07530"
                     /EC_number="3.1.1.103"
                     /inference="COORDINATES: protein motif:HMM:NF012371.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="serine hydrolase domain-containing protein"
                     /protein_id="HWQ84400.1"
                     /translation="MNIKPFILPSLLAAGGLALARRVIAAPRLTLAPAAPAVIPAAMP
                     AVMAAAKSDGKPSAMPAAAQAGYHSAYAALDAYVESQMRRLNIPGISLAVVSGSRIVH
                     QRGFGLARPGGQTPTPQTPFFIGSLTKSLTALAVMQLVEDGKLSLDTPVQDYLPWFRM
                     ADPQASARITIRHLLNQTSGLPCLPGELVLTDFDRQPGALERQMRALARLRLSHLPGE
                     TFEYSNLNFNLLGLVITASSGEDYAAYVQKHIFDPLAMRHSYTSKAAAQRHGLAVGHR
                     HWFGRPYPAPNLPVPAGSLASGQLISCAEDLAHYLIAQLNGGRYGDVQILSPAGIAEL
                     QRGAADNLIFGTAMGKYAMGWFEIDLGGTRTCSHTGNVPEYSAYMALVPGQHTGLVLL
                     ANSGTYGLPPITGELGMGLLALLAGQPPAPIRLDFIQRIMRGLPLLPILQLAGAAACL
                     RRLCRLDSDLDHRPSRGRLQQHLLFPLLPDLALASALVYLRASGLLSFLRLYMPDLAW
                     IARLSGGFAASWAILRTALVARALELEKRNPR"
     gene            complement(3763..4281)
                     /locus_tag="VN363_07535"
     CDS             complement(3763..4281)
                     /locus_tag="VN363_07535"
                     /inference="COORDINATES: protein motif:HMM:NF016001.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitroreductase/quinone reductase family protein"
                     /protein_id="HWQ84401.1"
                     /translation="MKLEKEIEMSASPTIPPYINSAVTTILRSPLHGLFSQSFLLLTF
                     TGRKSGRTFTTPVGYSQSGDTLTLFTHARWWKNLLNAAPVTLHLRGREIQGVPEPVTG
                     QEAILAGLLARLRRVPRDAGFYGVTIDNRGYPDLAETIRAVQSVVMIRIRLETSFVRQ
                     SSPQLGDAALEH"
     gene            complement(4315..4803)
                     /locus_tag="VN363_07540"
     CDS             complement(4315..4803)
                     /locus_tag="VN363_07540"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methyltransferase"
                     /protein_id="HWQ84402.1"
                     /translation="MLNILSRPNGLNIVGQGGKIILFTLPALAAAILAQLYIPRFAAL
                     PAWLGLLKPAGYLLLAPGLLLWGAAVIQLLTGFSRGSLVTTGAYGVVRNPIYASVIWF
                     ILPGVALLTLSWVYLVPAACLYLGVLIFIGVEERQLLQVFGSQYQVYLTRVDRLIPFK
                     NP"
     gene            complement(4838..>5122)
                     /locus_tag="VN363_07545"
     CDS             complement(4838..>5122)
                     /locus_tag="VN363_07545"
                     /inference="COORDINATES: protein motif:HMM:NF012770.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ84403.1"
                     /translation="VMDRLNEIKVPCLVMAGREDFLFPPEHQAILADRLPDARLELIE
                     RAGHNPQIERTAEVLAAIRSFLAGASARDEARLRDALPVPARTIDLAEGA"
BASE COUNT          991 a         1487 c         1790 g          854 t
ORIGIN      
        1 ggggtgtggg cggcggagat aaagccgtgg cgaggggaga tatgcagccc attcttgcgg
       61 aacagcaggc gtaagccggc gctggcggcg ggatctgcct gccaggcatc ccgcacgctc
      121 tggatcgggc cgggagcagc gtgcatccag aacatggagc ctaccccggt ggcgagggca
      181 ggaaaatcct gttcccgggc aaaacggttg atctcatccc gaaaacaatc acccaaggca
      241 ttgatgcgca ggtacaggtc gggatcaccc tgcaggcggc gcaggactgc cgtcccggcg
      301 gcggtgacca gcgggttgcc gttgaaggtg ccggccatca ggatcgctcg ctgcggctcg
      361 agatcgtatt ccagcaggcg catggctgcg gcagtgcccc cgatggcgcc aaaaggcagg
      421 ccacccccgg cgactttgcc gtaggtgctc aggtccggga tgaccccata gtactccttg
      481 gcaccgccag gcgcgaggcg aaatccggta atcacctcgt cgaagatgag caatgcgccg
      541 gtctgttggg tgaggcggcg cagctcggcc aggaaatcgg ggtcggccgg gatggtgccg
      601 gcggcgccct ggacgggctc gatgatcaca gccgccagcc ggtccgcatg cctgcggatc
      661 ttctcgaagg cggcgggatg gttgaacggc aggacgatcg tatcctgcca ggcgccggct
      721 gccagccccg gactctcggg gatggcggtg gggtcttcaa tgggaccata ggcatccatg
      781 tccagcccag gccggccgta gctgaccgcc agcaaatcgt gggcgccgtg gtagccgccc
      841 tcgaatttcg ccaccagggg ccggccggtg gcggcgcggg ccaggcggag ggcatacagg
      901 gtagcttcgg tacccgaatt gcagaaggtc actttttcga ctcccggcac catctcgcag
      961 aacagctgag caaactcata ttcgtgctcg tgacactgcc cataggaagt tccgcggggc
     1021 agcgcctcgc gcagggcggt ttcgacctct tcaggcgcgt tgccgagcag catggtcgag
     1081 aagcccagca tgtagtcgag gtattcgtta ccgtcggcat cccacaggcg cgccccacgg
     1141 gcagatttca ccaccatcgg gaagggatcg gacaggcgga agaggctgcc gacgccggcg
     1201 ggcatgacgg tgcgattttg ctgccagatg gccagcgagc ggggactgcg ggccatgatc
     1261 tcggcccggt agccgttaag cagtttttcg atttgttcag gatcagccat tgggaaactc
     1321 cttaaggtcg gttagcacgc atcagcccgc ggcgagcaga ccttccccgc actcgatcag
     1381 ctccaccagc cggttggtga atcgggctgc tggggcgcca tcgacgatat cgtgatcgaa
     1441 gagcaccgtc agattgagga tctcgcgcgg ttcgatgcga cctgcaacga ctgccggctt
     1501 ccaggcgatg ctgcccacca ccaggccgag cgagtgcggg gtggcggcga tgccccagcc
     1561 gctgtgatcc ttgccaaaca tcccgaccgc agttacacat acggttcccc cggcagccac
     1621 gcgcacggcc gggttccggc gggtggacag gttcagccag gctttcacca gcctggagag
     1681 cggccagggc aggagcagaa tcgagcgaaa ccaggcaggc atcccccggt tgggcggcac
     1741 cggggccgac tgggcggtgc ggatctcggc gtggatttcc gggtaagtct tgcggttggc
     1801 agcctggacg atcagcccca tcagggcgct tttttcgcca gccttatgtt caaccatcaa
     1861 gccgacattg acatcgtcaa acagcatgag ctgactgtgg cctttgcgat aagcctggag
     1921 gtctttatgc tcagccaccg cctgcgccag gcagtagacc aggaagccgg tgaaggacag
     1981 cgcctcgcct gtgcgagctt tgtgatcggc aatgagctgg cgggggaggg tcacatccac
     2041 ttccaggagc ccgtacatcc agtgggaggg cacggaaagg tccagggtat tcagccaggc
     2101 acggcgcccc ggggtaagct caacgacatg ggtgggtccg agattgtcaa tcatcgtgga
     2161 ttccttttct cgagctccag cgccctggcg accagcgcgg tgcgcaggat cgcccagctc
     2221 gcggcaaagc ctccgctgag gcgggcgatc catgccagat cgggcatgta caggcgcagg
     2281 aaggagagca ggccgctggc ccgcagatag accagggcgg atgccagcgc caggtccggc
     2341 aggaggggga agagcaggtg ctgctgtaga cggccgcggg atggccggtg atccaggtcg
     2401 ctgtccaggc ggcacagccg gcgcaggcag gcagcggcgc cagcaagctg caggatgggc
     2461 agcaaaggca ggccgcgcat gatccgctgg atgaaatcca gccggatcgg agccgggggc
     2521 tgaccggcaa gcagggcgag cagacccatg ccaagctccc cggtgatggg gggcaggccg
     2581 taagtaccag aattggcgag caagaccagg ccggtgtgct gacccgggac gagggccata
     2641 taggctgagt attcgggtac gttgccggtg tgggagcagg tgcgcgtgcc tcccaggtcg
     2701 atctcgaacc agcccatggc atatttaccc atcgcagtac cgaatatgag gttatccgcg
     2761 gcgccgcgct gcagctcggc gatgcccgcc ggcgacagaa tctgcacatc cccataccgc
     2821 cccccgttga gctgggcgat caggtaatgg gccagatctt cggcgcagga gatgagctgc
     2881 cccgatgcga gcgatccggc cgggacgggc agattgggag cggggtaagg gcggccgaac
     2941 cagtggcggt ggcctacagc gaggccgtgc ctttgggcgg cagccttcga ggtatagctg
     3001 tggcgcatcg cgagcgggtc aaaaatatgc ttctggacgt aggcggcata atcctccccg
     3061 ctgctggccg tgataaccag ccccagcagg ttgaaattga ggttgctgta ttcgaacgtc
     3121 tcgccaggta ggtgggagag cctgagcctg gcaagggcgc gcatctggcg ttccagcgca
     3181 ccgggctgcc ggtcgaaatc ggtcaggacc agctcgccgg gcaggcaggg caggccgctg
     3241 gtctggttga gcagatggcg gatcgtgatc cgggccgatg cctgcgggtc tgccatccgg
     3301 aaccagggca aatagtcctg gaccggggta tccaggctga gcttgccgtc ttcgaccagc
     3361 tgcatcactg ccagggcggt gagcgacttg gtgagtgagc cgatgaagaa aggcgtctgg
     3421 ggagtgggcg tttggccgcc cgggcgcgcc aggccaaaac cgcgctggtg cacaatccgg
     3481 ctgcccgaaa caactgcgag cgagatgccg gggatgttca ggcggcgcat ctggctttca
     3541 acataggcat ccagcgctgc ataggctgag tggtagccag cctgcgctgc ggctggcatt
     3601 gcagacggct ttccatctga ttttgcagcc gccattacgg ctggcattgc ggccggtatt
     3661 acagccggcg ctgcgggggc aagcgtcaga cggggggcgg cgatcacccg gcgagccagc
     3721 gccaagccgc cggctgccag caaagacggg agtatgaacg gtttaatgtt caagggctgc
     3781 atctcctaac tgcgggctgg actgccgcac aaaggaagtc tccagacgga tgcggatcat
     3841 caccacagat tgcaccgcgc ggatggtttc tgccaggtcc gggtaaccgc ggttgtcgat
     3901 ggtgacgccg tagaaaccgg catcacgggg cacacggcgc agccgggcca gcagaccggc
     3961 caggatggcc tcctgaccgg tgaccggttc cggcacaccc tggatctccc ggccccgcag
     4021 gtgcagggtg accggcgcag cattgagcag gtttttccac cagcgggcat gagtaaacag
     4081 ggtcagcgta tcgccagact gagaataacc gaccggggtc gtgaaggttc gcccgctctt
     4141 gcgcccggtg aaggtgagca ggaggaagga ttggctaaac aggccgtgga ggggcgaacg
     4201 caggatggtg gttacggcgg agttaatata cggagggatg gtagggcttg cggacatttc
     4261 gatctctttc tcaagtttca tgaaacttac tgaaataaag ctgggggtgc gccgtcaggg
     4321 gtttttgaag gggatcaagc ggtccacccg ggtgagataa acctggtact ggctgccaaa
     4381 gacctgcagg agctggcgct cttccacgcc gatgaaaatc agcacgccca ggtacaggca
     4441 ggctgccggg acaaggtaga cccagctgag ggtgagcagg gcgacgccgg gcaggatgaa
     4501 ccagatcaca ctggcgtaga tggggttgcg aactactcca taagcgccgg tagtcaccag
     4561 gctgccgcgc gaaaagcccg taagcagctg gatgaccgcc gctccccaca gaagcaggcc
     4621 gggcgccagg agcagatagc cggcgggttt gagcaatcca agccaggccg ggagggcggc
     4681 aaaccgggga atatacagct gagcgaggat cgcggcagcc agagcgggca gggtgaagag
     4741 gatgattttg ccgccctgac cgacgatatt cagtccgttt ggtctggata agatattaag
     4801 catggaattt ctcctaatgg ctttcatggg gctttgttta cgctccttca gcgaggtcga
     4861 tggttctggc agggactggt aacgcatccc tcaagcgtgc ttcatccctg gcactggcac
     4921 cggcgaggaa gctgcggatg gcggcgagca cctcggcggt gcgctcgatc tgagggttgt
     4981 gcccggcgcg ctcgatcagc tccaggcggg catccggcag gcggtcggcg aggatggcct
     5041 ggtgctccgg cgggaagaga aaatcctccc ggccggccat tactaggcaa gggactttga
     5101 tctcgttgag gcggtccatc ac
//
LOCUS       DATMXK010000407         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_120838, whole genome shotgun sequence.
ACCESSION   DATMXK010000407 DATMXK010000000
VERSION     DATMXK010000407.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120838"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VN363_07550"
     CDS             complement(<1..483)
                     /locus_tag="VN363_07550"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWQ84404.1"
                     /translation="MKKRAQAWLEILITWVIKISGASAILFVLVIFLFLMREGLPTLL
                     EVPFGDLFGSRWYPIEDYFGLLPLLGGSLLVTFGSALVAVPFGVGTAIFIAEIAPRWA
                     REILKPFIEVMAGIPSVVLGFLGMVVIAPGLRVLLNIPTGLTALTGALLLGGISIPTI
                     V"
     gene            complement(497..1378)
                     /locus_tag="VN363_07555"
     CDS             complement(497..1378)
                     /locus_tag="VN363_07555"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558694.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PstS family phosphate ABC transporter
                     substrate-binding protein"
                     /protein_id="HWQ84405.1"
                     /translation="MRLSLPLLYAFLALVLVGCASSNAGAAGSPPTTFIENKGSDTMV
                     NLALAWAEAYQSVEPQVSVSVTGGGSGTGITALANNTVDIANASRAIKDEEIAAMQKN
                     GIDPLEFTVARDAIAVIVNLNNPVERLTLPQISDIYSGKITNWSEVGGEDRPIVRLSR
                     ETNSGTHVYFLEEVLRLGDSDNETFFSANTLLLPSSEGITAEVRDNPNAIGYDGLGYV
                     VPEVKVLAVAAAGGDYFYPSVDTVNDLTYPIARDLYMYTAGAPSDVEQAYLDWIFSAP
                     AQAIVAELGFVPVSGQP"
     gene            1475..2245
                     /gene="pstB"
                     /locus_tag="VN363_07560"
     CDS             1475..2245
                     /gene="pstB"
                     /locus_tag="VN363_07560"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017979518.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWQ84406.1"
                     /translation="METPPSPIIQSQNLNFYYGAFKALEQIDMDILPQKVTALIGPSG
                     CGKSTFLRCLNRMNNTIPGTRVEGKITLEGQDIYAEGMDVTELRKRVGMVFQKPNPFP
                     QSILDNVAFGPRVLGMVQGKNGLDELLERSLRQAALWDEVKDKLGGSALELSLGQQQR
                     LCIARVLAVYPEVILMDEPCSALDPIATGHIEDLIAELKAEYTIVIVTHNMQQAARVS
                     DSTGFFWLGKLIEFTETPTLFTTPKEELTETYITGRAG"
BASE COUNT          643 a          646 c          866 g          463 t
ORIGIN      
        1 gacgatggtg gggatggaga taccgccgag cagcagggcg ccggtgaggg cggtgaggcc
       61 ggtggggatg ttcagcagga cacgcaggcc gggggcgatg acgaccatgc ccagaaagcc
      121 cagcaccacg gaagggatgc ctgccatcac ttcgatgaag ggtttcagga tctcccgcgc
      181 ccagcgggga gcgatctcgg cgataaagat ggcggtgcca accccaaatg ggaccgcgac
      241 cagggctgag ccgaaggtga cgagcagcga gccgcccagc agggggagca ggccgaaata
      301 atcttcgatg ggataccagc ggctgccgaa cagatcgccg aagggcactt cgagcagggt
      361 tggcagcccc tctctcatta aaaagaggaa aattaccagc acgaacagga tcgccgaagc
      421 acctgagatt ttaatgaccc aggtaatgag tatttcgagc caggcctggg cgcgtttttt
      481 catgctctgg gcgatcctac ggctgcccgc tcacggggac aaaacccagc tcagcgacaa
      541 tcgcctgggc gggggctgag aagatccaat ccaggtacgc ctgctccaca tcagaaggtg
      601 cgccggcagt gtacatgtac agatcgcggg cgatcgggta ggtgaggtca ttgaccgtgt
      661 ccaccgacgg gtagaagtaa tcacctccgg cggcggcgac tgccaggact tttacctcgg
      721 ggacgacata gcccagccca tcataaccga tggcattggg attgtcgcgc acttcggcgg
      781 tgatgccttc agaggagggc agcagcaggg tattggcaga gaaaaatgtc tcattatcag
      841 aatcgccgag gcgcaggact tcttcgagga aatagacatg ggtgcctgaa ttggtctcgc
      901 gtgacaggcg cacaatgggg cgatcttcgc cgccgacttc gctccagttg gtgatcttgc
      961 cactgtagat atccgaaatc tggggcaggg tgaggcgctc aaccgggtta ttgaggttga
     1021 cgatcacggc gatggcatcc cgggcaacgg tgaattcgag cggatcgatg ccattttttt
     1081 gcatggcggc tatttcttcg tccttgatgg cacgcgaagc attggcgata tccaccgtgt
     1141 tattggccag ggcggtgatg cctgtgccgg agccgccgcc ggtgaccgag acactgacct
     1201 ggggctccac cgactggtag gcttcggccc aggcgagggc caggttgacc atggtatcgg
     1261 agcctttatt ctcgatgaag gtggtgggtg gagagcccgc ggcacctgca ttggaagagg
     1321 cacagccgac caggacgagg gccagaaatg catacagcag ggggagtgaa agccgcatgg
     1381 acaaattata gtaggattaa gaaaagcgaa caagccctgg tgaaaagctg cgcaaaagga
     1441 taggcgcaaa gcagaattcg attaaaatag gctcatggaa accccacctt ccccaatcat
     1501 tcaatcccaa aatctgaact tttactacgg cgccttcaag gctctggaac agatcgatat
     1561 ggatatcctg ccgcagaaag tgaccgccct gatcgggccg tcgggctgcg gcaagtctac
     1621 attcctgcgc tgcctgaacc ggatgaacaa cacgatcccg ggaacccgcg tggaggggaa
     1681 gatcaccctg gaaggccagg atatctacgc ggaaggcatg gacgtgaccg agctgcgcaa
     1741 gcgggtgggg atggttttcc agaagccgaa tccgtttcca cagtcgatcc tggacaatgt
     1801 ggcgtttggg ccgcgggtgc tggggatggt gcagggtaag aatggtctgg acgagctgct
     1861 ggagcgcagc ctgcgccagg cggcgctgtg ggatgaggtt aaggacaagc tggggggcag
     1921 cgcactggag ctctccctgg ggcagcagca gcgattgtgc attgcccggg tgctggcggt
     1981 ctacccggag gtgatcttga tggacgagcc gtgttcggcg ctggacccga ttgccacggg
     2041 gcatatcgag gatctgatcg ccgagctgaa ggcagagtat acgattgtga tcgtgaccca
     2101 caatatgcag caggcggcgc gggtgagcga ttctacgggg ttcttctggc tggggaagct
     2161 gatcgagttt accgaaacgc cgacgctgtt tacgacaccg aaagaagagc tgacggagac
     2221 gtatattacg gggcgggccg gctagccgaa gacggctgac aacgaatgcc tgtaaagagc
     2281 acaggtggaa gaaaaatata accgcagatg acgcagattg gcgcagataa gagattggaa
     2341 cacgaaaagc acgaaaaagc acgaaaggta ggcgtaggat aagctagcct tgggttcagg
     2401 cttgcttgtc cggggaaatg ttaaccgcag atgacgcaga ttgacgcaga taagagattg
     2461 gaacacgaaa agcacgaaaa agcacgaaag gcacgaaagg tgggtgtagg ataagctagc
     2521 ctggagttca ggctcgcttg tccggggaaa tgttaaccgc agatgacgca ggttggcgca
     2581 gataagagat tggaacacga aaagcacgaa aaagcacg
//
LOCUS       DATMXK010000408         2378 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_94493, whole genome shotgun sequence.
ACCESSION   DATMXK010000408 DATMXK010000000
VERSION     DATMXK010000408.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2378)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2378)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2378
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94493"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..858)
                     /locus_tag="VN363_07565"
     CDS             complement(<1..858)
                     /locus_tag="VN363_07565"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ84407.1"
                     /translation="MKPTSLAEIIYLNAYWVGLSFMWNSLHVIILPAVLLNYVPESQK
                     NSYLGILTFFGLIIAMVIQPVSGALSDRWVSRWGRRRPLMLIGTAFDFVFLAILGWAG
                     GLLWLAIGYIGLQFSSNIAHGPAQGLLPDRVPAGQLGSASGVKNLMDMAGLVAASLLM
                     GRLVPPDIVLAIRPLGVVMAVLAVAALVTLVGVRESPSTRGSSIGSNLSSDLIEQSLI
                     NPVGKPRASRAYWWLIASRFVFLLGIYDIQVFAQYYVRDVIRTSNPVQLTGDLLAVIT
                     LGLIVFALLG"
     gene            complement(864..1568)
                     /locus_tag="VN363_07570"
     CDS             complement(864..1568)
                     /locus_tag="VN363_07570"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84408.1"
                     /translation="MLRHRPLSFRAEALQSVQRIRPGDSAAQPFLVSGSEYIPPSGPC
                     LLVANHYTRPGFKSWWLVLSISALVPFEVHWVVSAGWNFAGRQVARLTRVLFPRMARV
                     YGFTAMPPMPPDPEEVIGRAIAIRQVLNYLRTAPQPVVGLMPEGEDEELGILKMPHPG
                     TGRFMLLLAQSGLEVVPAGIYEAAGSLCVRFGPHIYLDTGSRLAAVERDRQASCQVMC
                     AIAGLLPEGMRGAFGR"
     gene            1762..>2378
                     /locus_tag="VN363_07575"
     CDS             1762..>2378
                     /locus_tag="VN363_07575"
                     /inference="COORDINATES: protein motif:HMM:NF015690.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF309 domain-containing protein"
                     /protein_id="HWQ84409.1"
                     /translation="MAQTRIESAARAAGFEVAWIESQEQIAPGVVEHPEPQRAEHVHG
                     PGYFLLEEITRRQPALLIFDLGNAGVPWREWIGLLRAVAATRRIPLICFGSHVDVESF
                     TAAKKAGAQAVLARSAFFGDLPGNLHKYARIPDREAMANACQEPLHARARKGLELFNA
                     GEYFEAHEELEAAWNADPGPGRALYQGVLQVAVAYLQIERGNPDGA"
BASE COUNT          570 a          721 c          697 g          390 t
ORIGIN      
        1 acccaggagg gcaaagacga tcaaccccag ggtgattacc gccagtagat cgccagtaag
       61 ctggaccggg ttcgaagtac gaataacatc ccgcacatag tattgtgcaa aaacctggat
      121 atcgtatatg cccaacagga aaacaaagcg cgaagcgata agccaccagt aagcccttga
      181 ggcgcgcggc ttaccaaccg gatttatcag cgattgttct atcaggtccg aggatagatt
      241 ggacccgata ctggatcccc ttgttgaagg actttcccga acacccacca gggtaaccag
      301 tgcagccaca gccaggacag ccataacgac ccctaaaggc cggatcgcaa gaacgatgtc
      361 cggaggcacc agcctgccca tcagcagtga agccgcaacc agtccagcca tatccatcag
      421 gttcttaacc ccgctggcgc tgcccaactg cccggcagga acgcggtcag gcagcagccc
      481 ttgagccggt ccatgggcaa tattggagct gaactgcagg ccaatatagc caatcgccag
      541 ccacagcaga ccgccagccc agcccaggat ggccaggaag acgaaatcaa aggccgtgcc
      601 gatcagcatc agcgggcggc ggcgccccca acgagaaacc cagcgatcac tcagcgcccc
      661 ggagactggt tggataacca tggcgatgat caacccgaag aaggtcagga tacccagata
      721 gctgtttttc tggctctcgg gtacgtagtt cagcaggaca gccggtagga tgatcacgtg
      781 caggctgttc cacataaagg aaagcccaac ccagtaagca ttcaagtaga tgatttcggc
      841 taatgaagtg ggtttcatct ttttcagcga ccaaacgccc cgcgcattcc ctccggcagc
      901 aggccggcga tagcgcacat gacctggcag ctggcctggc gatcgcgttc aactgcagcc
      961 agtctggacc cagtatccag gtaaatgtga gggccaaaac gaacacacaa cgagcctgcc
     1021 gcctcgtaaa tgcctgccgg aaccacttcc aatccagatt gtgccagcag cagcataaaa
     1081 cgcccggtgc cgggatgggg cattttcaga atgcccaatt cttcatcctc cccttcaggc
     1141 atcagaccaa ccacgggctg cggtgcagtg cgcagatagt ttaggacttg gcggattgcg
     1201 atcgctcgcc caataacttc ctccggatcg ggcggcatcg gcggcatcgc cgtgaagcca
     1261 tagacgcgag ccatgcgggg aaacaggacc cgggtgaggc gcgccacctg ccgcccagcg
     1321 aaattccagc ccgcactgac cacccaatgc acctcaaacg ggacaagagc cgagatgctc
     1381 aaaaccagcc accatgattt aaagccgggc cgggtatagt gattcgcgac cagcaggcag
     1441 ggaccggatg gagggatata ttctgaacct gataccagga aaggttgggc agcgctatcc
     1501 ccggggcgaa tgcgctggac ggattgcaag gcttcagccc ggaaactgag cggacgatgg
     1561 cgcagcatgg ctgtgctcag cctgaataag gtgctccaaa gaatgggata gtggggatgc
     1621 atgcgcggtc aattatactt gcccatcatc cggatcacga atcgaatgat caacagcgag
     1681 tcactgcctg ccagacgcgg gtataatcga accatgaatt cacaccaaaa ccctgtgatt
     1741 attggcttcg tgagcgaatt gatggcccaa acccggatcg agagcgctgc ccgggctgcc
     1801 ggatttgagg tagcatggat cgaatcgcag gagcaaatcg ctcccggtgt ggtagagcac
     1861 cccgaacccc agcgcgccga gcatgtccac ggaccgggct atttcctgct ggaggagatc
     1921 acccgccgcc agccggcgct gctgatcttc gacctgggga atgccggcgt accctggcgg
     1981 gagtggatcg ggctgctgcg ggcagtggca gccacgcggc gcatcccgct gatctgcttc
     2041 ggctcgcacg tggacgtgga aagcttcacg gcggcgaaga aagccggggc gcaggctgtg
     2101 ctggcccgct cggcattctt cggcgacctg cccggcaacc tgcacaaata cgcccgcatc
     2161 cccgatcggg aagctatggc aaacgcctgc caggagcccc tgcacgcccg cgcccgcaaa
     2221 ggcctggagc tgttcaatgc cggcgaatat ttcgaagccc acgaagagct ggaagctgcc
     2281 tggaacgccg accccggccc cgggcgggcg ctgtaccagg gcgtgctgca ggtggcggtg
     2341 gcatatttgc agatcgagcg cggcaatcca gacggggc
//
LOCUS       DATMXK010000409         2634 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_43017, whole genome shotgun sequence.
ACCESSION   DATMXK010000409 DATMXK010000000
VERSION     DATMXK010000409.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2634)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2634)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2634
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43017"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..595)
                     /locus_tag="VN363_07580"
     CDS             complement(<1..595)
                     /locus_tag="VN363_07580"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84410.1"
                     /translation="MLSTRKVLHTWWPLAASWILMGLEGPTLSAIVARLANPEINLAA
                     WGGIVNPLSLIIEAPIMMLLAASTALSKDWASFQKMYRFMMLAGASLTGLHVLVAFTP
                     LYDIVVSGILGAPPEIVEPGRIGLRIMLPWTWAIAYRRFHQGVLIRFNHSGVVGRGTL
                     VRLGSLVMILAIGYILNRAPGILVGTAAVAVAVTLEAI"
     gene            807..1400
                     /locus_tag="VN363_07585"
     CDS             807..1400
                     /locus_tag="VN363_07585"
                     /inference="COORDINATES: protein motif:HMM:NF036525.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWQ84411.1"
                     /translation="MMTRDDILNAAAQVFSQKGFHAASMQDIAEAVNLQKGSLYHHVN
                     SKQEILALLLDHALDLLIKRLEAVIAQPLPTEGKLRLAIAEYLTTILEQKELAAVLLL
                     EHRNLDPELAARHNLARDQFEGLWRSLVRQGMEEGVFCCMDPVIAVMAIMGTINWTIT
                     WYRPSGALSPAQIADQFAGLILSGMLTRPPSPPIPQY"
     gene            1494..2498
                     /locus_tag="VN363_07590"
     CDS             1494..2498
                     /locus_tag="VN363_07590"
                     /inference="COORDINATES: protein motif:HMM:NF012955.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ84412.1"
                     /translation="MPLELFPGFWGYAYLVLVDLPGSPSMQVLIDSGSGLDSSNEHLE
                     AGLQAVSEVVDLPVGLPDLTHVLITHGHIDHFGGLSYIRPRSQAEFGVHELDWRIVAN
                     YEERLVVAARRLEGFLIEAGINPERRSAMLDLYQLTKGLYRSVDADFTYEACGMRVGP
                     FELLHVPGHCAGHVVIRLDDVLFSGDHILSEISPHQSPERITLSTGLSHYLHSLSALR
                     SWGQGIELVLGGHRAPVDSLEQRIAEIESLHHQRLNQVLDILRQPGTVSDVSQALFGK
                     VHGYNVLLAVEEAGAHVEYLYQHGRLAIENLSEVENNPTPIPIVYRLLSGPKCSEALT
                     "
BASE COUNT          629 a          705 c          677 g          623 t
ORIGIN      
        1 aaatcgcttc gagtgtaacg gcgacggcga ccgcagctgt gccaaccaga ataccggggg
       61 cgcgattcag aatatagcca atggcaagga tcataaccag actgcctaat cggaccaggg
      121 tgcctctccc tacaaccccc gaatgattga accgaattaa aaccccctgg tggaaacggc
      181 ggtaagcaat cgcccaggtc cagggcagca taatccttaa gccgatcctg cctggctcga
      241 caatctcggg aggtgcaccc aggatgccgc tgaccacaat atcatacagc ggggtgaatg
      301 ccaccaggac atgcagcccg gtcaggcttg cgccggcgag catcatgaat ctgtacattt
      361 tctggaaaga cgcccaatcc ttacttaaag ccgtcgaagc tgccagaagc atcatgatcg
      421 gtgcttcgat aatcagcgac aggggattga caatgccacc ccaggctgcc aggttaatct
      481 ccgggttcgc caggcgggcg acgattgcgc tgagggttgg cccttccagg cccatcagta
      541 tccagctggc agccagcggc caccaggtat gtaaaacttt gcgtgtagat aacaaaaagg
      601 ttgccccggg ataggatcag atctttattg agttcatgaa tgggttattg tattccaaaa
      661 cccagcctgg tcatagggaa atgctgcaaa acacccccca atagcataca gtctcgcatt
      721 cagtaactgg gagttgaaca aatattaact tgactccgcg gcttagaaat gatatcctta
      781 aactaccaaa cgtttggtag ggagggatga tgacacgcga tgatattctc aatgccgctg
      841 cgcaggtttt ctcccaaaaa ggtttccacg ccgcttcgat gcaggatatt gccgaagcag
      901 tcaatttaca gaagggcagc ctgtatcacc acgtcaacag taaacaagag atcctggcat
      961 tactgctgga tcacgccctg gatttattga tcaaacgact ggaagcggtg attgcccaac
     1021 ccttgcccac cgaaggcaag ctccgtctgg caattgccga gtatctcact acgatcctgg
     1081 aacaaaaaga attagctgca gttttgctgc ttgaacaccg caatcttgat cctgaattgg
     1141 ccgcccgcca taatcttgcc cgcgaccagt ttgaaggatt atggcgcagc ctggtccggc
     1201 agggaatgga ggagggtgta ttttgctgca tggatcctgt aatcgccgtg atggcgatta
     1261 tggggacgat caactggaca attacctggt accggccctc gggagccctt tcgcccgctc
     1321 agatcgccga ccagtttgct gggcttatcc tatccgggat gctgacccgc cctccatcgc
     1381 cgccaatccc acagtattga aatttgatgg ctacgaaatt agaacccatt ccaacatctg
     1441 gctcagcaca gatccaggca tttaacagcc gctctggagc gcgtattttc cagttgccac
     1501 tggaattatt tcctggtttt tggggttatg cctacctggt cctggttgat ctacccggga
     1561 gcccctctat gcaggtattg atcgactccg gttctggcct ggacagctcc aatgagcacc
     1621 ttgaagctgg tttacaggcc gtaagcgagg tagtagatct gcctgtcggt cttccggatc
     1681 ttacccatgt tctgattacg catggacata tcgatcactt tggcgggctg agctatatcc
     1741 ggccgcgttc acaggcggaa ttcggtgtcc atgagctgga ttggcggatc gtcgcaaatt
     1801 atgaagagcg tctggttgta gctgctcgcc ggttagaagg atttctaatc gaggctggaa
     1861 ttaacccgga acgccgcagt gcgatgttag acctgtatca attgaccaaa ggtttgtatc
     1921 gctcagtgga tgcagatttt acttatgaag cctgtggtat gcgcgtgggt ccttttgagt
     1981 tactccatgt gcccgggcac tgtgccgggc atgttgtcat ccgcctcgat gacgtgttgt
     2041 tttcgggcga tcacatcctg tctgaaatta gccctcacca atcgccggaa aggatcacgc
     2101 tctcaaccgg tttatcccat tatttacact ccctttctgc tctacgcagc tggggccagg
     2161 gcatcgagct cgttctgggt gggcacagag caccggttga ttcacttgag cagcgaattg
     2221 ctgagataga aagcctccac caccagcgcc tcaaccaggt attggatatc ctgcgccagc
     2281 caggaacggt cagcgatgtc tcccaggcgt tatttggaaa ggtacatgga tacaatgtcc
     2341 tgctggcagt cgaagaagcc ggcgcgcatg tggaatattt ataccagcac ggcaggctcg
     2401 ccatcgaaaa cctgtccgaa gtggaaaata atcccactcc gatccctatc gtctatcgcc
     2461 tgttgtctgg accgaaatgc tcagaagcac ttacctgatc ctggaggtgc cttatcccgt
     2521 ccttttttcg ccaacccgtg aatccgcttg atccgaattc aaaggaggag ctatatgtac
     2581 tcgttcgaac cagacgaaga acaaaaaatg cttgtggata cgatcagccg ctat
//
LOCUS       DATMXK010000410         5489 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_6309, whole genome shotgun sequence.
ACCESSION   DATMXK010000410 DATMXK010000000
VERSION     DATMXK010000410.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5489)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5489)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5489
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6309"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..269)
                     /gene="rpsO"
                     /locus_tag="VN363_07595"
     CDS             complement(<1..269)
                     /gene="rpsO"
                     /locus_tag="VN363_07595"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_003719603.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S15"
                     /protein_id="HWQ84413.1"
                     /translation="MSLDKDTKNQLIVEFSRHEGDTGSPEVQVALLTKRINQLTDHLR
                     ANSHDESSRRGLLKMVGQRRRLLAYIRRNDFHRYMALTDKLSIRLS"
     gene            440..1351
                     /gene="ftsY"
                     /locus_tag="VN363_07600"
     CDS             440..1351
                     /gene="ftsY"
                     /locus_tag="VN363_07600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560069.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005047 - signal recognition particle
                     binding [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal recognition particle-docking protein
                     FtsY"
                     /protein_id="HWQ84414.1"
                     /translation="MSIFDKWKTGLAKTSKSTFGQIATFFGATQITEATWDELEGILV
                     QADLGVETTLEVIASLQERIYNQGLTRASELREAMRAELRARLDTPPVVDFSSQPTII
                     LVVGVNGSGKTTTIAKLGKRYAEEGKRVILGAADTYRAAAVDQLQVWADRLDLPVIAG
                     QTGGDAGAVAYDTVQAAINRKADLALIDTAGRLHTRFNLMEELKKVHRVTSKAMPGAP
                     HHIWLVMDATTGQNAMQQARAFKEAVQVTGIILAKLDSSARGGMAFAIQRELGLPIYY
                     AGLGEKPSDLTPFDPDAFVAGILDAAG"
     gene            1403..2471
                     /gene="prfB"
                     /locus_tag="VN363_07605"
                     /pseudo
     CDS             1403..2471
                     /gene="prfB"
                     /locus_tag="VN363_07605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_113918868.1"
                     /note="programmed frameshift; incomplete; partial in the
                     middle of a contig; missing C-terminus; Derived by
                     automated computational analysis using gene prediction
                     method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="peptide chain release factor 2"
     gene            2566..5097
                     /locus_tag="VN363_07610"
     CDS             2566..5097
                     /locus_tag="VN363_07610"
                     /inference="COORDINATES: protein motif:HMM:NF042625.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6541 family protein"
                     /protein_id="HWQ84415.1"
                     /translation="MARGEAQQNSRSLLKLVLLLSSLALLLSACTTLTDFDSTQDYRG
                     QVVGVVSRESTVGQSLVVRRPDFNGIVLWLRPKDTEYPGDAALQVRILEAPGAEEALF
                     EQSLSFSRLEAQFPVTIAFPPLSDRPEESYYLELSTPDGEVNVMGRLENASPRGEAYL
                     SGEPMDADLSFRLLYRYDLKDAWEDLMARVGEIWLLAPLLLTVWLPGWLLLQISGLGT
                     NLERGERWALAVGLSLAVIPLVPLWTSLLGLRWTGVTVWLAMGALVVAAGWWIYQHNF
                     HPGRLPAQVEPDRLETSSGESEPVPDRANALPWIPMALVAILLVSFFLRMAMVRDLAA
                     PPWVDSVHHGVITRLIMEDGQYPTDYEPYLEIGTAHYHPGFHALLALFTSLSGLEMER
                     SMLIFGQALNALAILGVYLFTVTLTGQRLAGLIAALIAGFLTPMPAYFTTWGRYTQLA
                     SLTILPTAFTLLTRLVLDKPRQRTDLLPGGHTFRLLLLTGIACGGLFLTHYRVAAFLF
                     LLVLAWMLVRSRTTVIETGRANKLGLEAGLLAGGGSAALLLSLPWWPGLISTLIGPSL
                     AWGSTAAKFFDGFTWSFLTSAQGTPVLWLAGAGLLLGLLTRRNFPLVLVIWTGLLFFI
                     ANLAALKLPGGGLVNTISVEISLFLPLAAAAGYFVAWVLNAAGSLLPKNLAWLPRLAL
                     LIGSLGVVWLGFRDLLPVLNPVTVLYRSADEQAMDWMRDNLPAGSEVQINPFYWGYNL
                     YAGADGGYWIGPLAGKSTNPPPVLYGIDPSPEADPLATTDLNRQVLDLAGQPDELYRL
                     LSSQGIEYVYLGARGGGLSPTALLQNQHFKLLYAGNLTYVFEVGP"
     gene            5181..5480
                     /locus_tag="VN363_07615"
     CDS             5181..5480
                     /locus_tag="VN363_07615"
                     /inference="COORDINATES: protein motif:HMM:NF013677.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA];
                     GO_process: GO:0006313 - DNA transposition [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWQ84416.1"
                     /translation="MVERRKFSPEFKARVVLELISGKKGLMEASREYGIKDTVLSRWK
                     QEFLERAAQIFEQPKPNGQEKEIAELERIIGRLTIQLDMAKKVLGYSGFPPKENE"
BASE COUNT         1153 a         1622 c         1660 g         1054 t
ORIGIN      
        1 gacaggcgga tgctcaactt atcggtgagg gccatatatc ggtgaaaatc attccgccgg
       61 atgtaagcca gcaaacgccg gcgctgaccg accattttca gaaggccgcg gcgagaagat
      121 tcatcatgcg aattggcacg caaatgatca gtgagctggt taatgcgctt ggtcagcaaa
      181 gcaacctgga cctcaggaga accagtatcg ccttcatggc gtgaaaattc gacgatcagc
      241 tggttttttg tatccttatc gagagacatg acgtctgcac tccttgtata atgatctagc
      301 aggtctccag ggctgtagct cgccacgttg ggcaaaccgc agaccggtct agtcaaagcg
      361 aaaggattat accagagaga cgaaagaagg acaaggcagc gctcaccctt cgaaacagcg
      421 catgttataa tttcagccta tgagtatctt cgacaaatgg aaaaccgggc tggcgaaaac
      481 cagtaaatca actttcggcc agatagccac cttctttggg gcaacccaga tcaccgaagc
      541 cacctgggac gagcttgagg gcatcctggt gcaggccgat ctgggcgtgg aaaccacact
      601 ggaagtgatc gcatcacttc aggaacggat ctacaaccag ggtttgaccc gtgccagtga
      661 gctgcgcgaa gccatgcgcg ccgagctgcg cgccaggctc gataccccgc cggtagtaga
      721 tttcagcagc cagccgacca tcatcctggt ggtgggcgtg aatggatcgg ggaagaccac
      781 caccatcgcc aagttgggga aacgctatgc cgaggaaggt aaacgcgtga tcctgggggc
      841 agccgatacc taccgggcag ccgccgtgga ccagctccag gtgtgggcgg accgcctgga
      901 tttgccagtg atcgccgggc agacgggggg tgatgccggg gccgtcgcat atgacacggt
      961 tcaggcggcg atcaaccgca aagccgacct ggccctgatc gacactgccg ggcggctgca
     1021 cacccgcttc aatctgatgg aagagctcaa aaaggtacac cgcgtgacga gcaaagcgat
     1081 gcccggcgca ccccaccata tctggctggt catggacgcc accaccggac aaaatgccat
     1141 gcagcaagcc cgggcgttta aggaagccgt ccaagtgacc gggatcatcc tcgccaagct
     1201 ggattcatcg gcgcgcggcg ggatggcctt tgcgatccag cgcgagctgg gtttaccgat
     1261 ctactacgcc gggttgggcg agaaaccttc cgacctgact ccttttgatc cggacgcgtt
     1321 tgtcgccggg atcctggacg cagccgggta atttttcgga caatttcctg gcttcacgca
     1381 atccctgctt attggagtaa ccatgcaaga cctacaagaa cgcttatcta acgctcaaaa
     1441 ccgcatccag cacatcctgg agcgtctttg acgtaccttc cttagtaatc gagctcaaaa
     1501 aactacagaa agcctccgaa gatcccgacc tgtgggataa cccggataat gcccagaaga
     1561 tcatgaaaaa gctggcctcc gtccgcgaag atgtggcgga ctggctggga ttcgagcagc
     1621 gcatcaacga caccctggag ctggctgaaa tggaagacga gagcctgcgc gctgagctcg
     1681 agccggaatt ggcagccatc gaaaaggagc tggaacagcg cgagttcaaa gccatgctct
     1741 ctgggccata cgacaaaggc aacgccttac tggcgatcca tgccggggct ggcgggactg
     1801 actctcagga ttgggcgcag atgctggagc ggatgtacct gcgctggtgc gagctgcgca
     1861 acttccagag cgagatcctc gatctgaccg agggcgaaga agccgggatc aaaagcatta
     1921 ccattgcagt gaacggctcc tacgcgtttg gatacctgcg tcctgagaaa ggcgtgcacc
     1981 gtctggttcg cctgtcgccg ttcgatggcg ctcaccgccg ccatacctct ttcgccctgg
     2041 tcgaagtcct cccccaggtg gaagatgaca gtgaggtgga gatcaacgcc agcgacctgc
     2101 gcatcgatac ctatcgctcg gctggcgcgg gtgggcagaa tgtgcagaaa aacgataccg
     2161 cgatccgcat cacccacctg cccagtggga ttgtggtcac ctgccagaac gagcgctcgc
     2221 aggtgcaaaa ccgggaaaat gccatgcgcg tgctgcgggc tcgcttgagc gagcttaagc
     2281 gcgccgagca ggatgagcaa atggcgatcc tgcgcggcga atacaccaaa gccgaatggg
     2341 gcagccagat ccgctcgtat gtgctgcatc cctatcaaat ggtgaaagat caccgcaccg
     2401 attatgagac aggcaacact caggctgtac tggacgggga aattgatggc tttatcgaag
     2461 cctacctgcg cgccaacctg ggtcagaagg ccagtcctga ggccagccct aaggccagcc
     2521 cgaaagccag cccgaaagcc agcccgaagg ctagcacggc caaaaatggc caggggtgag
     2581 gcgcagcaaa actcacgcag tctattaaaa ctggtcctgc tgctgagcag cctggcgctg
     2641 ctcttgagcg cctgcaccac cctgaccgac ttcgacagca cgcaggatta ccgcggccag
     2701 gtggtgggcg tggtcagccg ggagtccacc gtcgggcaga gcctggtggt gcgccggccc
     2761 gacttcaacg gcatcgtgct ctggctgcgc cccaaagaca cagaataccc cggcgatgcg
     2821 gccctgcagg tgcgcatact ggaagcgccg ggcgctgaag aagccctgtt tgaacaatcg
     2881 ctcagcttca gccgtctcga agcccagttc ccggtcacga tcgcgtttcc acccctctca
     2941 gatcgccccg aagaaagcta ttacctcgaa ttatccaccc cagatggcga agtaaacgtg
     3001 atgggccgcc tggagaatgc ttcgccgcgc ggcgaggcgt atctctccgg cgagcccatg
     3061 gacgcggacc tgagtttccg cttgctctac cgctacgatc tcaaagacgc ctgggaagac
     3121 ctgatggcgc gggtgggaga gatctggctg ctggcccccc tcctgctgac ggtgtggctg
     3181 ccgggctggc tgctgctgca gatcagtggg ctgggaacga atctcgagcg cggcgagcgc
     3241 tgggcactgg cggttggcct gagcctggca gtgatcccgc tcgtcccgct gtggacttca
     3301 ctgctcgggc tgcgctggac cggcgtaacc gtatggctgg caatgggcgc cctggtcgtg
     3361 gcggctggct ggtggatcta ccagcataat tttcaccccg gtcgcctgcc ggctcaggtg
     3421 gaaccagaca ggcttgaaac cagcagcggt gaaagcgagc ctgttcccga ccgggcaaat
     3481 gcccttccct ggatcccgat ggcactggtt gctattctgc tggtgagctt cttcctgcgc
     3541 atggccatgg tgcgcgacct ggctgcgcct ccctgggtgg attcggtcca tcatggggtg
     3601 atcacgcgcc tgatcatgga ggatgggcag tatcccacag attatgagcc ctacctggag
     3661 atcggaaccg cccactacca tcccggcttt cacgcccttt tagccctatt taccagcctg
     3721 tccggtttgg agatggagcg cagcatgctc atcttcgggc aggctttgaa tgccctcgcc
     3781 attctgggtg tctatttatt taccgtcacc ctcaccgggc agcgcctggc aggcctgatc
     3841 gcggccctga tcgcagggtt cctcaccccc atgccagcct atttcaccac ctggggccgc
     3901 tacacccagc ttgccagcct cacgatcctg cccacagcct tcacattatt gacccgcctg
     3961 gtattggata aaccccggca gcgaacagac ctgctccccg gggggcatac cttccgtttg
     4021 ctcctgctca cagggattgc ctgcggaggg ctgttcctca cccattaccg ggtggccgcg
     4081 ttcttattcc tcctggtcct ggcatggatg ctggtgcgca gccggaccac cgtgatcgaa
     4141 accggccgcg cgaataaact gggattggaa gccggcctgc tggcaggggg cgggtcggca
     4201 gctctgctgc tcagcctgcc ctggtggccc gggctgatca gcaccctgat cggccccagc
     4261 ctggcctggg ggagcacagc ggcgaagttt ttcgatggct ttacctggag ctttctgacc
     4321 tccgcccagg gcacgccggt gctgtggctg gcaggggcag gcctgctgct gggactgctc
     4381 acccggcgta attttccact ggtcctggtg atctggaccg ggttgttgtt cttcatcgcc
     4441 aatctggccg cgctgaagct gcccggcgga ggattggtca acaccatctc ggtcgagatc
     4501 agcctgttcc tgcccctggc agcggcagca ggttatttcg tggcctgggt gctcaatgcc
     4561 gcggggagcc tgctccccaa aaacctcgcc tggctgccgc gcctggccct gctgatcggg
     4621 tcgctgggcg tggtctggct gggcttccgt gacctgctgc cggtgcttaa cccggtgacg
     4681 gtactgtacc gcagcgcgga cgagcaagcc atggattgga tgcgggataa tttgcctgcc
     4741 gggtcagaag ttcagatcaa tcccttctac tggggttaca acctctacgc cggggcggat
     4801 ggaggctact ggattggccc gctggcggga aagagcacca acccgccccc ggttttatat
     4861 ggcatcgatc cctccccgga agcggatcca ctggcgacca cggacctgaa ccggcaggtg
     4921 ctggacctgg caggccagcc ggacgagctg taccgcttat tatccagcca ggggatcgag
     4981 tatgtctacc tcggcgcgcg gggaggggga ttatcgccca cagcgctgct ccaaaaccag
     5041 catttcaagc tgctgtatgc cgggaatctg acgtatgtat ttgaggttgg gccgtgaacc
     5101 tctctcatca ccctgtagtg gatcgataaa actggacaca ccccttaggg gtggggtaca
     5161 cttcaggaaa gaggtccact atggtagaac gacggaagtt cagtccagaa ttcaaagctc
     5221 gtgtcgtgct ggagctcatc tcgggtaaga agggcttgat ggaagccagt cgagagtatg
     5281 gcatcaaaga cacggtgctc tcgcggtgga agcaagaatt cttagaacga gcggcccaga
     5341 ttttcgaaca gccgaaaccg aatggccagg agaaggaaat cgccgagcta gagaggatca
     5401 tcgggcgatt aacgatccag ttggacatgg caaaaaaagt attgggatac tccggcttcc
     5461 cgccgaagga aaacgagtaa tggtgagag
//
LOCUS       DATMXK010000411         2536 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_140448, whole genome shotgun sequence.
ACCESSION   DATMXK010000411 DATMXK010000000
VERSION     DATMXK010000411.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2536)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2536)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2536
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140448"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(34..1599)
                     /locus_tag="VN363_07620"
     CDS             complement(34..1599)
                     /locus_tag="VN363_07620"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWQ84417.1"
                     /translation="MPVSGSTIQQTNIVTENKSQKSNFIWLWLPALLLMALGIGLRLY
                     DMSDPPLDFHATRQLRGAIIARSMYYQMLPGADPAQVQIAEQLANSTGQYEPSILEKI
                     VAYIYLWTGGESAWAARVVNTIFWAVGGIFIVLLSRRINQGSNPFAVLPALVALAYYL
                     VLPFGVQASRSFQPDPGMVMWIILGIYALYRWSETHSWKWALLAGLFGGVAILVKAVA
                     AYIMAGAAISLVLYTVGWRRFWRSAQVWAMMLMMVLPSVFYYLLLRGGRASEYFSSWT
                     LSLSHLLLDPATYVRWLSVVADLIGLGALLLGLLGIVLAYGRGRALLVGLWAGYLVYG
                     LLTPYQMYTHSYYHIQLVAIVAISLVPAVHALARQVNSQPSKWRAAAIGIFVLGIGFA
                     SWEAVLPQYRDDYRHEPAYWQEIGNHLPSDGKILALTQDYGYRIMYYGWRKVILWPNR
                     GEIALSELRGEGKVFEDYFNRRSQGMNYFLITAFKQLNDQPDLEEHLEQHYPVYAEGS
                     GYLIYDLAHPLQP"
     gene            complement(1583..2395)
                     /locus_tag="VN363_07625"
     CDS             complement(1583..2395)
                     /locus_tag="VN363_07625"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559082.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-hydroxymethylpterin diphosphokinase MptE-like
                     protein"
                     /protein_id="HWQ84418.1"
                     /translation="MSSFRQSVKQIIPNPIWQAGRSSWHALGHAAQWPAATLHPWRRA
                     SIEQLARLKDIHRGQRCFIIGNGPSLKKTDLTHLKDEYTFGSNRIYLAFPEWGFTTSY
                     LLSINSLVIEQCAEEIRSLPIPKFISWRSRSLIQPTPDLAFLHTTYNGPRFSTDVRGR
                     VWEGGTVTYVALQLAFHMGFSQVILIGVDHSYVTQGTPNATVVSQGDDPNHFHQGYFG
                     KGFRWQLPDLEMWERSYQMARQAYAQAGRQVLDATIGGKLAVFEKIDYASLW"
     gene            complement(2392..2529)
                     /locus_tag="VN363_07630"
     CDS             complement(2392..2529)
                     /locus_tag="VN363_07630"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ84419.1"
                     /translation="MIGRVGKPEEIANAIYFLADESQSSFMTGQALVVDGGATARLST
                     E"
BASE COUNT          629 a          687 c          706 g          514 t
ORIGIN      
        1 ctgaaaccag gggcaagttt tctgagattg gcatcagggc tgcagggggt gggcgaggtc
       61 gtagatcagg tagccggagc cttcagcgta aaccgggtaa tgttgttcca ggtgctcttc
      121 cagatcaggc tgatcattca actgcttaaa ggcggtgatc aggaaataat tcataccctg
      181 gctgcggcgg ttaaagtaat cttcgaatac tttgccttcc ccacgcagct ccgagagggc
      241 aatctcgccc cggttcggcc acaggatcac cttgcgccag ccataataca tgatccggta
      301 gccataatct tgagtgaggg ccagtatttt accgtcggag ggtagatgat tcccaatctc
      361 ctgccagtat gcaggttcgt ggcggtaatc atcccggtat tggggtaaga cggcctccca
      421 gctggcaaat ccgattccca gtacgaatat gccaatcgca gctgctcgcc atttggaggg
      481 ctgagaatta acctgcctgg caagcgcatg tacagccggg acaagagaaa tcgccacaat
      541 cgccacaagc tggatatgat aataactgtg cgtgtacatc tggtaaggcg tgagtaatcc
      601 atagaccaga taacctgccc ataacccgac taacagtgcg cggcctcgcc cataggccag
      661 gacgataccc agcaggccca ggagcagtgc ccccagcccg atgaggtccg cgaccacgct
      721 gagccagcgc acataagttg ccgggtcgag cagtaaatga gataaagata gtgtccagga
      781 cgaaaaatat tcgctggccc gcccgccccg taaaagcaga taataaaata cggacgggag
      841 caccatcatc agcatcatcg cccacacctg agcgctgcgc caaaaacgac gccatcccac
      901 agtgtacagc accaggctga tcgccgcgcc cgccataatg taggccgcga cagctttgac
      961 caggatcgcg acacccccaa acagacccgc caggagcgcc catttccagc tgtgagtttc
     1021 agaccagcga tacaaggcgt atattcccag gatgatccac atcaccatgc ccggatcagg
     1081 ctgaaaggaa cggctggcct gtactccaaa gggcaggacg agataataag ccagggcgac
     1141 gagggcgggt aaaactgcga aaggattgga tccctgatta atccgccggc tgagcaggac
     1201 gataaagatt ccaccgaccg cccaaaatat tgtattcacc acccgagcag cccaggcgct
     1261 ttcgccgcca gtccacaggt aaatataggc gacgattttt tccagaatgg atggttcgta
     1321 ctgcccggtc gaattagcca gttgttcagc gatctgaacc tgggctggat cggcgcctgg
     1381 aagcatctgg taatacatac tgcgggcaat gatggccccc cgcagctggc gtgtggcatg
     1441 gaaatccagc ggcggatcgg acatatcata caggcgcagg ccaatgccca aggccattaa
     1501 taacagggct ggtagccaga gccagatgaa attagatttt tgggatttat tctcggtcac
     1561 aatgttggtt tgttgaatag tgctaccaga gactggcata atcaatcttt tcgaaaaccg
     1621 ccagcttgcc gccaatagtg gcgtccaata cctggcgacc agcctgagca taagcctggc
     1681 gcgccatctg gtacgagcgt tcccacattt ccaggtcggg aagctgccag cgaaaacctt
     1741 tgccgaaata gccctggtgg aaatgattgg gatcatcccc ctgggagaca acggttgcat
     1801 tcggtgtacc ctgggtgacg tagctgtgat ctacaccgat caggatcacc tgggagaaac
     1861 ccatgtgaaa agccagctgg agtgctacat aggtaacggt gccgccttcc catacacgcc
     1921 cgcgcacatc cgttgaaaac ctgggcccat tgtaggtagt gtgcaagaaa gccagatcgg
     1981 gagtgggctg gatcaggctg cgtgagcgcc aggaaatgaa tttagggatg ggcaaggagc
     2041 ggatctcttc agcacactgc tcgattacga ggctgttgat cgagagcaga taactggtcg
     2101 taaatcccca ttccgggaag gcgagataaa tgcggtttga gccaaaggtg tattcatctt
     2161 tgagatgggt cagatcagtc tttttcaggc tcgggccgtt gccgataata aaacagcgct
     2221 gcccgcgatg aatgtctttc agcctggcca gctgctcgat cgatgctctc cgccagggat
     2281 gcagggtggc tgccggccat tgggcggcat gacccagggc atgccagctg ctgcgcccag
     2341 cctgccaaat tggattggga ataatttgtt tgacagactg gcggaaactg ctcattctgt
     2401 gctcaagcgg gcggttgcgc cgccatcaac aaccagggcc tggccggtca taaaggagga
     2461 ctggctttca tcagccagga aataaatcgc gttcgcgatt tcctcaggct tgccaacccg
     2521 tccgatcaca gtcttg
//
LOCUS       DATMXK010000412         2789 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_65800, whole genome shotgun sequence.
ACCESSION   DATMXK010000412 DATMXK010000000
VERSION     DATMXK010000412.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2789)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2789)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2789
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_65800"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2747)
                     /gene="cydD"
                     /locus_tag="VN363_07635"
     CDS             complement(<1..2747)
                     /gene="cydD"
                     /locus_tag="VN363_07635"
                     /inference="COORDINATES: protein motif:HMM:TIGR02868.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055051 - ATP-binding cassette (ABC)
                     transporter complex, integrated substrate binding
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0033230 - ABC-type cysteine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0033228 - cysteine export across plasma
                     membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiol reductant ABC exporter subunit CydD"
                     /protein_id="HWQ84420.1"
                     /translation="MWLIGSLAQSLTHRQWKSLSRMSAYFLDVIQGLETLKALGRSRA
                     QIDVIAQVSDNFRQTTLSVLRVTFLSALALELVSTLSTAVVAVQIGLRLLYGRLTFEE
                     AFFVLLLAPEFYLPLRNLGTRFHAGMSGISAARRIFSILDIPRSVPDQPATASPDFTA
                     PIRMDNLHFYYSDHRQALDGASFTLPPGETVALVGPTGAGKSTVVQLLLGFSRPQRGH
                     ILIGDHALDEISLESWRAGIAWVPQKPYLFYGSIADNIRLGRPEASHSEIIHAGRLAH
                     ADGFISSLPHGYDTIIGENGATLSGGQAQRIALARAFLQDAPLLILDEPTANLDPSTE
                     AALQDSLALLLKGRRALIIAHRLSTVKHADRIVVMEKGKVVEQGTHAELLRLDGLYRR
                     LVGAYRDEPDQLPPGRQTAHRSVENQPVEVISNSARPRRSSAPISNKTVFLRLLGLVR
                     PHWKDVALSVVLGFATIASSIGLLSASAYIIASAALQPSVAVLQVPIVGVRFFGIARG
                     VFRYLERLVSHNVTFQILSDLRVSFYRALEPLAPARLQRYRSGDLLTRLTADIETLEN
                     LYVRALAPPLVAVLVGLVSVAYLAGYAVSLAAALSFLLVLCGLCIPWLARQLSKSPGQ
                     RIISLRAQLNTLVVDSLQGMPDLLAFRQARSQEDRLSQLSHELAAAQGRMAQITGLQS
                     GLTVLLSNFGLWWVLRLAIPLVNDGIFSGVYLPVVGLAALTSFEAVQSLPSAAQHLES
                     CIHAAKRLLEVVDADPEIVDPPSPLELPAQPSLIAYRLGFTYPSRLDSDEEVSFGLDE
                     LNFDLIPGKKIALVGPSGAGKTTLVSLLLRFWEYDEGNLYYAGTEVRQLRPEDLRAKI
                     GLVSQNTYLFTTTIRENLLIANPDATQAQIDAAIHQAQLGDFIHSLPEGYETWLGGQA
                     QR"
BASE COUNT          700 a          776 c          776 g          537 t
ORIGIN      
        1 cgctgggcct gcccccccag ccacgtttcg tagccttccg gcaaagaatg gataaaatca
       61 cccagctgcg cttggtggat ggctgcatcg atttgcgcct gggtggcatc cgggttggct
      121 atgagcaggt tttcgcggat cgtggtagta aataaatagg tgttctgaga gactaatccg
      181 atcttggcgc gcaggtcttc cggacgtagc tggcggactt ccgtgccagc ataatataaa
      241 tttccctcat catattccca aaaccgcagc agcaggctta ccagggttgt tttgcctgcc
      301 ccactcggac caaccagggc aatcttttta ccaggtatca gatcgaaatt cagctcatcc
      361 agtccaaagc taacttcctc atcggaatcc agacgagaag gataggtaaa tcccaaccgg
      421 tatgcgatca gtgatggttg ggctggcagt tcgagcgggg atggtggatc gacgatttca
      481 ggatcagcat cgaccacttc aagtaaacgc ttcgccgcat gaatgcagct ctccagatgt
      541 tgggccgcag atggcaagga ctgtaccgcc tcaaagctgg tcagggccgc cagaccaacc
      601 actggcagat agacacccga gaaaattcca tcattaacca ggggtatggc caggcggagc
      661 acccaccaca atccaaaatt tgaaagcagg acggtcaagc cagattgcaa accagtgatc
      721 tgcgccatcc ttccctgggc agctgccagc tcgtggctga gctgcgaaag acgatcttcc
      781 tggctccggg cctgacggaa agctaataga tcaggcatgc cttgcagact atccaccacc
      841 agtgtattta actgggcacg caggctgatg atgcgctggc caggggactt gctgagctgg
      901 cgcgccagcc aggggatgca caacccacac aggaccagta ggaaggacag cgcagctgcc
      961 agactcacgg cataacctgc cagataagca accgaaacca accctaccaa cacagccacg
     1021 aggggtggcg ctaaggcgcg tacatacaga ttttccaggg tttcgatatc agcggtcagg
     1081 cgagtcagaa ggtcaccgct gcggtagcgc tgcagccggg ctggagcaag cggctccaat
     1141 gctcgataaa acgagactcg taaatcagac aagatttgaa aggtcacgtt gtgggaaacc
     1201 agccgttcca aatatcgaaa tacaccccgc gcgatcccaa agaagcgtac tcccacgatc
     1261 ggtacttgca gcacagctac cgagggctgt agtgccgcgc tggcgataat atatgcggaa
     1321 gcgctcaaca ggccgatgct gcttgcgatg gtggcaaaac ccaaaaccac cgagagcgcc
     1381 acgtctttcc agtgggggcg gaccagcccc aaaaggcgca agaagacggt tttattagaa
     1441 atcggggcag agctgcgcct tgggcgagcg gaattcgaaa tgacttcgac tggctgattt
     1501 tctacagacc tgtgcgcggt ctgcctgcca gggggaagct ggtctggctc atctcggtaa
     1561 gcacccacca gcctgcggta gagtccgtcc agcctcagca gctcggcatg tgtgccttgc
     1621 tcgactactt ttcctttttc cattacaaca atacggtcgg cgtgcttcac cgtggaaaga
     1681 cggtgagcaa tgattaacgc ccttcgccct ttaagtaata gcgccaaaga atcctgcagt
     1741 gccgcctcgg ttgaagggtc taaattggca gtcggctcat ccaaaatcaa cagcggggca
     1801 tcttgcaaga aagcgcgggc cagggcaatc cgctgcgcct gtccaccgct caacgtagcc
     1861 ccattctcgc caattattgt gtcataacca tggggcaaac tggagataaa cccatcagcg
     1921 tgtgcgagcc gcccagcatg gattatttca gagtggctgg cttctggtct gcccaggcgg
     1981 atattatcgg caatactgcc ataaaacaaa tacggcttct gcggcaccca ggcaatcccc
     2041 gcacgccagc tttccagaga aatttcatcc agggcgtgat caccgatgag gatatgcccg
     2101 cgctgtggcc gggagaatcc cagcagaagc tggaccacgg tactcttacc tgcgccggtg
     2161 ggccccacca gcgcgacagt ctcgcctggg ggcaaagtaa agctggcccc atccaatgcc
     2221 tggcgatggt cactatagta aaaatggagg ttatccatgc ggatgggtgc tgtaaaatct
     2281 gggctggctg tggctggctg gtctggaacg gaccgcggaa tatccaggat tgaaaaaatc
     2341 cgccgggcgg cggaaatgcc agacataccg gcatggaagc gagtccccag attacgcagg
     2401 ggcagataaa attcaggcgc caggagcaaa acaaagaacg cctcctcgaa tgtgagccgt
     2461 ccatacaata agcgcaggcc aatttggaca gccaccactg ccgtgcttaa ggttgaaacg
     2521 agctccagag ccagggcaga gaggaaggtt acccgtaaga cgctcaaggt ggtttgccgg
     2581 aaattgtcgc ttacctgagc aatcacatcg atctgagccc ggctccggcc gagggcttta
     2641 agcgtttcca gaccctggat cacatcaagg aaataagcac tcatccggct caaggatttc
     2701 cactgccggt gagtaagcga ttgagccaga ctgccaatca gccacatgaa aaggggaatc
     2761 agtggggcag tcagcagtaa taccaggcc
//
LOCUS       DATMXK010000413         2796 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_50238, whole genome shotgun sequence.
ACCESSION   DATMXK010000413 DATMXK010000000
VERSION     DATMXK010000413.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2796)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2796)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2796
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50238"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1078)
                     /locus_tag="VN363_07640"
     CDS             complement(<1..1078)
                     /locus_tag="VN363_07640"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ84421.1"
                     /translation="MFTRFFSKTYLYPILHSVISVTLVFLLIEFFDELNFGIEGAALP
                     VIRDDFGLTYTQIGLLFGAPHLISTLIEPVLMLLGDTRLRKSLIIAGGLMITLALLLI
                     AFGHSFPILLIAMLIAFPASGAFVSLSQATLMDLNPGRQAQLMARWTAAGSIGNLLGP
                     LILAGSLSLGWGWRWTFVALAGLAVVLVFSMLPQRLPHPASDLPTTSLLETFKYLGAG
                     LWSAIRNRQLLRWLAFLEMSDLMMDRFISFVPLYFTDVIKVSPAQASLLLTGIMVTSL
                     AADLIVIPLLEKIPGLRLVRASAFVCAGMYAAALLAPWLWVKMILFMLVSFTTLGWYP
                     VLQGEAYAAVPGRSGTVMAVNSLAG"
     gene            complement(1237..1383)
                     /locus_tag="VN363_07645"
     CDS             complement(1237..1383)
                     /locus_tag="VN363_07645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560458.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein"
                     /protein_id="HWQ84422.1"
                     /translation="MITGVSFRGQGLLEYALIMMLVAIVVIIALALLGPAIGNLYSNV
                     VPRI"
     gene            1595..>2796
                     /locus_tag="VN363_07650"
     CDS             1595..>2796
                     /locus_tag="VN363_07650"
                     /inference="COORDINATES: protein motif:HMM:NF012700.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84423.1"
                     /translation="MDLKTLTTLEYFKILEHLAGYTSFAVSGDKARALRPTADIIEAR
                     RRLAETSEAVQMLVTHADLSIGGARDIRAAVDLAVHGGVLAPVDLLDVKSTLIAARSL
                     ARRFERLAGQYPHLTEIVSRLPAPAGIVDAISKAISERGDILDDASPKLGAIRRELRI
                     TRDRLLSKLQRMVSDPKNTPMLQDNLVTQRDGRYVLPLRSEFKGRMRSIVHDQSSSGA
                     TLFVEPLAVVELNNQNRELQLAERDEERRILAELSQQVGLKAEELRWMLDGIAELDMI
                     LARAKYADDLQAIEPVLREFPAPQRKAEEGQARHPGSVIRLEQARHPLLDPQSVVAID
                     IELDPQTYTLVITGPNTGGKTVTLKTVGLLALMAQTGMLIPAHTGSEISCFEDIFADI
                     GDEQSIEQS"
BASE COUNT          715 a          748 c          789 g          544 t
ORIGIN      
        1 cgccagccag tgaattaaca gccatcaccg ttccagaacg accaggcaca gcggcataag
       61 cctcaccctg caggaccgga taccaaccca gggtcgtaaa actcaccagc ataaacagga
      121 tcatttttac ccacagccag ggagccaata aggctgctgc atacatgcca gcacagacga
      181 atgcgctggc gcgcaccagg cgcagaccgg gtatcttttc caggagcggg ataacgatca
      241 ggtcggcagc cagactggtg accatgatcc cggtcaggag cagactggct tgagcagggc
      301 tgactttgat cacatcggtg aaatacagcg ggacaaagct gataaaacgg tccatcatca
      361 aatccgacat ctccaaaaag gctaaccagc gcaagagctg ccggttgcgg atggcggacc
      421 atagaccagc tcctaaatat ttaaatgtct caagcagtga tgtggttggt agatcactcg
      481 ctgggtgcgg caggcgttgg ggcagcatgc tgaagacgag gaccactgcc aatccggcca
      541 atgccacgaa tgtccagcgc caaccccaac ccaggctcag actgccagcc aggatcagag
      601 gacccagcaa gttgccaatg gagcccgcgg cggtccagcg cgccatgagc tgagcctggc
      661 gaccggggtt aaggtccatc agggtagcct gggaaaggct gacaaacgct ccgctggccg
      721 gaaacgcgat cagcatggca attaacagga tcgggaatga atgcccaaaa gcaatcaaca
      781 gcagggccag agtgatcatc agaccaccgg cgatgatcag gcttttcctc agccgggtat
      841 cgcccaacag catgagaacc ggttcaatca gtgtgctgat gagatgaggg gcgccaaaaa
      901 gcagaccaat ctgagtgtag gtcaacccaa aatcatcccg gatgaccgga agggctgccc
      961 cttcgatacc gaaatttaat tcatcgaaaa attcgatgag caggaaaacc agcgtcaccg
     1021 agatgacgct gtggaggatc ggatacagat aagttttgga aaagaaacgg gtaaacacat
     1081 acacctcttt ctgtagatgc tggccgatgg atgtgggcaa agctgcccaa caaaccactg
     1141 cgtcgagaaa agaaagaggc ggtgcgcgtg ctgcgttagc agacgatagg atctaacggg
     1201 gcgggtggaa gatgttttcc atgaactgat tatccattaa attcggggaa caacattgct
     1261 gtatagattc ccgatcgctg gccccagcaa cgccaaagca ataatgacaa cgatggcgac
     1321 caacatcata atcaaagcat actcaagcag cccctgccct cgaaaactca ccccagtaat
     1381 cattgaaaac gtctcctgcc taagatcgag gttgcatgcc ctggaaagag gcgcgccctc
     1441 cattctgatc tatagtatag ctgattagga ttaaaaaaac aattgccagg tgcaattatc
     1501 ctacaatgtt gctgtgtccg ggatcaattt ttcgagtcac ctgacaaaat tgtatggtaa
     1561 actcgatttg ttctggaaca aggaccatcg acccatggac ttaaaaacac ttacgaccct
     1621 tgaatatttc aaaatcctgg aacacctggc aggctatact tcattcgcag tttcgggtga
     1681 taaggcgcgc gccttgcgcc cgaccgcaga tatcattgaa gcccgccgca ggctggcaga
     1741 gaccagcgaa gcggtacaaa tgctggtcac ccatgctgat ctgagcatcg gcggggcgcg
     1801 cgatatccgg gctgcggtgg atctggctgt ccacggaggg gtcctggctc cagtcgatct
     1861 gctggatgta aagtcgacct tgattgcggc gcgcagcctg gcgcggcgct ttgagcgact
     1921 ggcgggccag tatccccatc tcacagagat tgtcagccgc ctgccggcgc cggcagggat
     1981 cgtggacgct atcagcaagg cgatctctga gcgcggcgat atcctggatg atgcttcacc
     2041 caaactggga gccatccggc gtgaactgcg gatcacccgc gaccgcctgc tgagtaaact
     2101 ccaacggatg gtctccgatc cgaagaacac gccaatgctg caggacaatc tggttacgca
     2161 gcgagatggg cggtatgtct tacctttgcg ctcggaattc aagggccgca tgcgttcgat
     2221 tgttcacgac cagtcctcat ctggggcgac gttatttgtg gagcccctgg ctgtggtgga
     2281 gctgaacaac cagaaccgcg aactgcagct ggcagagcgg gatgaggaac gccggatact
     2341 ggctgaatta tcgcagcagg tggggctgaa ggcagaagag ctgcgctgga tgctggatgg
     2401 cattgccgag ctggatatga tcctggcccg ggcaaaatat gcggatgatt tgcaggcgat
     2461 tgaaccggtt ttgcgtgaat ttccggcccc gcagcgtaag gcggaggagg gtcaggctcg
     2521 ccaccccggc agtgtgatcc gcctggaaca ggcccgccat cccttattgg atccgcagag
     2581 cgtggttgcc atcgatatcg aactcgatcc tcaaacttat accctggtca tcaccggacc
     2641 caataccggc ggtaaaacgg tgaccttgaa aaccgtcggt ctgctggcat tgatggctca
     2701 gaccgggatg ctgattccag cccataccgg ctcggagatc agttgttttg aggatatttt
     2761 tgctgatatt ggcgatgagc agtcgatcga gcaatc
//
LOCUS       DATMXK010000414         2455 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_38947, whole genome shotgun sequence.
ACCESSION   DATMXK010000414 DATMXK010000000
VERSION     DATMXK010000414.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2455)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2455)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2455
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38947"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..319
                     /locus_tag="VN363_07655"
     CDS             <1..319
                     /locus_tag="VN363_07655"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWQ84424.1"
                     /translation="IGHILRKLSLDELPQFWNILMGDMSMVGPRPIVVDETQHYADRF
                     HWYASVRPGLTGLWQVSGRNNLTCAVRVDLDEYYVRNWSVWLDIYILLRTVWVVVRGD
                     GAY"
     gene            372..2066
                     /locus_tag="VN363_07660"
     CDS             372..2066
                     /locus_tag="VN363_07660"
                     /EC_number="2.7.10.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01007.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0045226 - extracellular polysaccharide
                     biosynthetic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="polysaccharide biosynthesis tyrosine autokinase"
                     /protein_id="HWQ84425.1"
                     /translation="MDNSSFSDDLRRYIGLLWHWAWLLALVTVLAAGAVYLISRSLTP
                     VYEASTTLLINEAQSNQTTDYTAILTSERLARTYAAMMTKQPVLESVMAELGLAMDLE
                     DLEEAVSVQPVRDTQLIEVTAEDNSPQRAADIANTIFQVFQAQNQALQASRFATSKES
                     LAAQLDEVNAQVNQTEADLAALPVGEQAERDRMESSLAQYRQTYAGLLTSYEEVRVAE
                     AQATSNVVQVEPAQAPLEPVRPRVAVNTLLAGLVGLMLAVGGIFLVEALDNTLRTPDD
                     VTRLLGLPVLGIIADHPVEDGHLVALDTPRSPVAEAFRSLRTNLQFASVDRPLVKLLV
                     TSPGPGEGKSTVAANLAAVLAQSGKRVTLLDADMRRPRVHKLYGLPNRKGLSELFVGP
                     RINLNGVLQGTQMENLSALTTGTLPPNPSELLGSEKMNEIIAQVMEVSDVLVIDSPPV
                     MLVTDSAVLSQRVDGILLVLRQGETNLEFARQSVEALRRVDANLLGVVLNGVPVRSSR
                     YGYYRYQRYDAAYADYYGDDTHKPGRARRRGLLRTSRRSEPDPAPAAKKADLNPRG"
     gene            2166..>2455
                     /locus_tag="VN363_07665"
     CDS             2166..>2455
                     /locus_tag="VN363_07665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84426.1"
                     /translation="MVAAVVAATFIDSRLLPLAVAVGLAFWPLRWQVTGRFTRRTPVD
                     WGIAGLLLMLPVSLWVTAYPQETRLQAMRLLSGILLFYSLVNWANSPTRLLWV"
BASE COUNT          412 a          740 c          794 g          509 t
ORIGIN      
        1 catcgggcat atcctgcgca agctcagcct ggacgagctg ccccagttct ggaatatttt
       61 gatgggggat atgagcatgg tggggccgcg cccgattgtc gtcgatgaaa cccagcatta
      121 tgcggatcgc ttccactggt acgccagcgt gcgccccgga ctcaccggcc tgtggcaggt
      181 ctcgggacgc aataatctga cctgtgcggt gcgcgtcgac ctcgacgaat attacgtccg
      241 caactggtcg gtctggctgg atatttacat cctgctgcgc acggtctggg ttgtcgtgcg
      301 cggggatggc gcgtactgaa cctgtaaaat ctccccggca tgctaaaata acgctggaaa
      361 gggtccttat tttggataat tcttcatttt cagatgatct gcggcggtat atcggtttgc
      421 tctggcattg ggcgtggctg ctggcgctgg tcactgtgct ggccgcgggg gcggtttacc
      481 tgatcagccg cagtctgacc ccggtgtacg aagcctcgac gactttgttg atcaatgagg
      541 cccaatcgaa ccagaccacc gattacaccg ccatcctcac cagcgagcgc ctggcgcgca
      601 cctatgccgc catgatgacc aagcagccgg tgctggaatc cgtgatggct gagctgggac
      661 tggcgatgga tctggaggac ctggaagagg cggtttccgt gcagccggtg cgcgacaccc
      721 agctgatcga ggtgaccgcc gaggataaca gcccccagcg cgccgccgat attgccaata
      781 ccattttcca ggttttccag gcccagaatc aggccctgca ggcctcgcgt tttgccacct
      841 ccaaggagag cctggcggcg cagctggatg aggtcaatgc ccaggtcaac cagaccgagg
      901 ccgacctggc agctttgccg gtaggcgagc aggccgagcg cgaccgaatg gaaagctccc
      961 tggcgcagta ccgccagact tatgccggac tgctgaccag ctacgaagag gtgcgggttg
     1021 ccgaagccca ggccacctcc aatgtggtcc aggtggagcc ggcccaggcg cccctggagc
     1081 cggtccggcc gcgggtggcg gtcaataccc tgctggcggg tctggtcggt ttgatgctgg
     1141 cggtgggcgg catcttcctg gtggaagccc tggataatac cctgcgcact cccgatgatg
     1201 tcacccgcct gctggggctg cctgtgctgg gtatcatcgc cgatcacccc gtggaggatg
     1261 gtcacctggt ggcgctggat acgccccgct cgccggtcgc cgaagccttc cgctctttgc
     1321 gcaccaattt gcagtttgcc agtgtcgatc gcccgctggt caagctgctg gtcaccagcc
     1381 cgggcccggg ggagggcaaa tcgaccgtgg ctgccaacct ggcagcggta ttggcgcaga
     1441 gcggcaagcg cgtcaccctg ctggatgcgg atatgcgccg gccgcgggtg cacaagctgt
     1501 acggcctgcc caaccgcaag ggcctgagcg agttatttgt cggcccgcgc atcaacctga
     1561 acggcgtcct gcaggggact cagatggaga atctgtcggc tctcaccacc ggaaccctgc
     1621 ctcccaatcc ctccgagctg ctcggctcgg aaaagatgaa tgagatcatc gcccaggtga
     1681 tggaagtcag cgacgtgctg gtgatcgatt ccccgccggt gatgctggtt accgattcgg
     1741 cagtcctctc gcagcgcgtg gatgggatcc tgctggtgct gcgccagggt gagaccaacc
     1801 tggagtttgc ccgccagagc gtggaagccc tgcgccgggt ggatgccaac ctgctgggag
     1861 tggtgctgaa cggcgtgccg gtgcgcagct cgcgctatgg ttattaccgc taccagcgct
     1921 acgatgctgc ctatgccgat tattatggcg atgatacgca caagcccggg cgtgcccggc
     1981 ggcgcgggct gctgagaacc tcccgccgtt ccgagccaga tcctgctccg gctgccaaaa
     2041 aagctgattt gaatccccgg gggtaagtgg tgtctgtgtc tgagacgcac gtctgggcgt
     2101 ctttttgctc aatacaggtc tgattaagat tgcttgcccg gcgtttactg gattatgagg
     2161 ttccgatggt cgctgcagtg gttgcagcga cttttattga ttcccgcctg ctgccgctgg
     2221 cagtggcggt tgggctggcg ttctggccgc tgcgctggca ggttacgggc cgctttaccc
     2281 ggcggacccc ggtggattgg ggcatcgccg gcttgctgct catgctgccg gtcagcctgt
     2341 gggttaccgc ttacccgcaa gagactcgcc tgcaggccat gcgcttgctg agcgggatcc
     2401 tgctgttcta cagcctggtc aactgggcga acagtcccac ccggctgctt tgggt
//
LOCUS       DATMXK010000415         8373 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_135646, whole genome shotgun sequence.
ACCESSION   DATMXK010000415 DATMXK010000000
VERSION     DATMXK010000415.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8373)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8373)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8373
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_135646"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(4..609)
                     /locus_tag="VN363_07670"
     CDS             complement(4..609)
                     /locus_tag="VN363_07670"
                     /inference="COORDINATES: protein motif:HMM:NF024319.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing protein"
                     /protein_id="HWQ84427.1"
                     /translation="METKGSFQLEKLAGCAGAEWVRTYFELSRLKQLYRQGWLKRGLS
                     RERCESVADHSFAAALLAYWLARAEFPELDAGRVLSMALMHDLGEVYVGDLIPQDKIN
                     PLTKHEMESQAVNQIFANLPEGDLYIQVWEEYEAASSPEARFVRQIDRLEMALQASLY
                     EHQGERNMQEFFDSAGQAVSDPRLRRIFDTLLSCRENQTGS"
     gene            complement(668..4219)
                     /gene="metH"
                     /locus_tag="VN363_07675"
     CDS             complement(668..4219)
                     /gene="metH"
                     /locus_tag="VN363_07675"
                     /EC_number="2.1.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559618.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008705 - methionine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0009086 - methionine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="methionine synthase"
                     /protein_id="HWQ84428.1"
                     /translation="MPEPKIKRSYLDAVEQRVVIFDGAMGTSLQGLKLTPEHFGGEDL
                     NGCNDVLVLTAPEMVEQVHRSFLDVGVDVIETDTFRANRLTLGEYGLADKVYEINFAA
                     ASLARRLADEYSTPTQPRFVAGSIGPSGKLPSADDPDLANVTFDELADIFCQQAVGLI
                     EGGADLLLIETSQDILEVKAALTGLQKAFTATGMRLPVQAQVTLDTTGRMLLGTDIGA
                     ALAILEGMPVDVLGLNCSTGPEHMRAPINYLGEHSRLPVSCIPNAGLPLNVDGEAVYP
                     LEPEPFAEALTEFVEKYHINVVGGCCGTTPAHLKLLVDRLGGQGAGRVSAPVRPVLSE
                     PFLASATQAAPMQQDPPPLLIGERCNAQGSRKFKRLLLEEDYDAILEIGREQVNGGAH
                     ALDISVAVTERTDEVQQMRQVVKKLALGVEVPLVIDSTEPEVMEAALQAAPGRCLLNS
                     THLEAGPAKAERIFGLARTYNAAVLVLTIDEQGMAKTARCKLEVAQRIYQMAVDTIGL
                     RPQDLVFDTLTFTLATGDPEFGNSAIDTLEGIRAIKASLPGVLTSLGVSNVSFGLSPA
                     ARPVLNSIMLYHAVQAGLDMAIVNPAQITPYADIPQAEREMAEDLIFNRRPDALQRLI
                     EYFEAAGPVVQDPSGKADPLAELSPAGRLQWRILHRHKDGVELDIDAILAENAALPPE
                     ERLTQHVAAVKILNEVLLPAMKEVGDKFGAGELILPFVLQSAEVMKKSVAHVERYLER
                     LEGTSKGVIVLATVYGDVHDIGKNLVKTILANNGYTVHDLGKQVPAETIIQKAVEVKA
                     DAIGLSALLVSTSKQMPLIVNELQRRGLKFPVLVGGAAINRRFGWRILYTEDRNRYDP
                     GVFYCKDAFEGLAVMDGLADPQKKPAVMDKLFQEAEHEILRSDRPAKAAPSSARRSQV
                     PAAKFIPVPASWGGRVVRQMPLELVFQHLSKNELYRLSWGAKNSRGEEWEQLQKEFDT
                     RLGKMQRQALQQGWLQPQGVYGYWPAQADGDALVVFDPQSLSSSKPQELMRFEFPRQP
                     AGDYLCLADYFTPLGSDPMDVVAFQVVTVGQAATDRFDKSHAAGDYSEAYYMHGLAVQ
                     TAEAAAEYLHRHIRRELGLAEDQGKRYSWGYPAIPEVSDHQKVFELLPAEKELGMSLT
                     AAYQLVPEQSTAAIILHHPAARYFSVGESRVEQLLKT"
     regulatory      complement(4280..4401)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00162"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="SAM riboswitch class I; Derived by automated
                     computational analysis using gene prediction method:
                     cmsearch."
                     /bound_moiety="S-adenosylmethionine"
                     /db_xref="RFAM:RF00162"
     gene            4552..5562
                     /locus_tag="VN363_07680"
     CDS             4552..5562
                     /locus_tag="VN363_07680"
                     /inference="COORDINATES: protein motif:HMM:NF024138.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWQ84429.1"
                     /translation="MEPIRRITKYDHLKILADSRRLYILQRLMAGPATLSMLGKELGE
                     HPARVRHHLKKLEEIGLVELVNTQVVRGFIEKYYRAKARAFLLNDLILPAETPPDHQT
                     LFIMGSHDLALEALIQHTQEKAHQTSYLSIPTGSLEGLVALRQGTAHFSGAHLWDAER
                     NEYNLSYVHYFFPDQDMVLLTLAHREQGLMVRPGNPRQIKNLVDLVRPDIILINRNRG
                     SGTRLWLDHQFHQLGIAGAQLQILQGDARTHTASAEAVGSGLADVGLGVHAAAQKAGL
                     DFIPLFQERFDLVMPAALLNSPAMQPLLNDLQSAEIRQIIRDLEGYDSTETGKQITPQ
                     PG"
     gene            complement(5602..6207)
                     /gene="recR"
                     /locus_tag="VN363_07685"
     CDS             complement(5602..6207)
                     /gene="recR"
                     /locus_tag="VN363_07685"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_008301521.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombination mediator RecR"
                     /protein_id="HWQ84430.1"
                     /translation="MLLPEPIQNLINAFSRLPGIGPKTASRLTFHLLRTSDDLPTDLA
                     QALSALKTSTTYCPICFNITMAGRQECEICGDAHRDSRMICVVEEPLDVLALERTAAY
                     PGRYHVLHGALSPIEGIGPDDLKIHELLERVRAGGIVEIILATNPSMEGDYTAAYLQQ
                     QLAASGVRLTRLARGLPVGGDLEYADQSTLLRALSGRQEMS"
     gene            complement(6238..6567)
                     /locus_tag="VN363_07690"
     CDS             complement(6238..6567)
                     /locus_tag="VN363_07690"
                     /inference="COORDINATES: protein motif:HMM:NF014616.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0008150 - biological_process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YbaB/EbfC family nucleoid-associated protein"
                     /protein_id="HWQ84431.1"
                     /translation="MAKGFKGPKVPNQMGMVQQLQKLQEQIQATQAQLATEIVSATAG
                     GGAIKVSVTGDQHCHAVEIDPEILKDGDVELLQDMVLTAFNSALDKSRAMAAERLGPL
                     AGGGLPF"
     gene            complement(6609..8192)
                     /gene="dnaX"
                     /locus_tag="VN363_07695"
     CDS             complement(6609..8192)
                     /gene="dnaX"
                     /locus_tag="VN363_07695"
                     /EC_number="2.7.7.7"
                     /inference="COORDINATES: protein motif:HMM:NF004046.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009360 - DNA polymerase III complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003887 - DNA-directed DNA polymerase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit gamma/tau"
                     /protein_id="HWQ84432.1"
                     /translation="MAQALYRKWRPQNWNEVVGQEHVIQTLHNALIAERIGHAYLFAG
                     PRGTGKTTTARLLAKAVNCLAEGLADRPCGVCAHCQAVTEGRFLDLIEIDAASNTSVD
                     DVRDLREKINFSPNQGRYKVYIIDEVHMLSTAAFNALLKTLEEPPSHAIFILATTEIH
                     KIPATVLSRCQRHEFRRIPVADIVAHLRKQAEQENIPVENDALTLIARQSTGALRDAI
                     SLLDQLASTGQTITLELTQNLLGTAASQAVLDVIEALIARQPDTGINTLQLALDSGSD
                     PRQYARQFVDYLRDLLLMRMGNADQIDATTETRAIMARHAQAYTPAELLRLIRLFNAA
                     ANESKNAWQPSLPLELAFIEALEPAGEPESDLNNQASAPRSTLTPRKTRPEAEAPAQT
                     SPAAVEAAPSPTPTEGQPLDDLTFKRVTEHWRRILQSIGQQSKQTQALLNSCKPFGMK
                     DGVLFLGFNGEFAKTKMEKGEHVDLARQVIQQVLGSDIPLRCFVVTGRGGSLPPDVES
                     DGMVAAAMRDLGGELVDVQ"
BASE COUNT         1862 a         2399 c         2210 g         1902 t
ORIGIN      
        1 ctattacgat cctgtttggt tttcacgaca acttaacagt gtatcgaaga tccggcgcag
       61 gcgcggatcg gagacagcct gaccggcact gtcgaagaat tcctgcatat tacgctcgcc
      121 ttgatgctcg tagaggctgg cctgcaaggc catttccagg cggtcgatct ggcgcacaaa
      181 acgcgcttcc ggtgagctgg cagcttcgta ttcttcccat acctgtatat acagatcacc
      241 ttccggaagg ttggcaaaaa tttgatttac agcctggctt tccatttcat gcttggtcag
      301 agggttaatc ttgtcctggg ggataagatc accgacatac acctcaccca gatcatgcat
      361 caatgccatg cttaatactc gtccggcgtc cagctctgga aattcagccc gtgccagcca
      421 gtaagccagc agcgcggctg caaaagaatg gtctgccaca ctttcacagc gctcgcgcga
      481 gagcccccgc ttcagccaac cctggcgata cagctgcttc aggcggctta attcaaagta
      541 agtccgcacc cactcggctc cggcacaccc agccagcttc tcgagttgaa aggaaccctt
      601 agtttccatc ctgatcctgc ctgatttgct tcgaatatcc ccggttagaa tccactcgct
      661 gacgaggtca ggtttttagc aattgctcca cccggctctc accgacggag aagtagcgcg
      721 ccgccggatg gtggaggatg atcgcagccg tggattgctc cgggaccagc tggtaggctg
      781 ccgtcaggct catccccagc tctttttccg cggggagcag ctcgaacact ttctgatggt
      841 ctgaaacctc tggaatagcc gggtagcccc aggaatagcg tttgccctgg tcttcggcca
      901 ggccaagctc gcggcggatg tgccggtgca gatactctgc agccgcctcc gcagtctgga
      961 cagccaggcc atgcatataa taggcctcag aatagtcccc ggcagcatgt gatttgtcaa
     1021 agcggtctgt ggcagcctgc ccaaccgtga ccacctgaaa agcgaccaca tccatcggat
     1081 cagagcccaa aggggtgaaa taatccgcca gacaaaggta atctccggct ggctggcgag
     1141 ggaattcgaa ccgcataagc tcctgcggct tgctggagct gagggattgc ggatcaaata
     1201 ctaccaatgc gtcgccatcc gcctgggctg gccaataccc atatacacct tgcggctgca
     1261 gccagccttg ctgcaaagcc tggcgctgca tctttcccag acgggtatca aattccttct
     1321 ggagctgctc ccactcttcg ccgcggctgt tttttgcacc ccaggagagg cggtacagct
     1381 cgtttttgga taagtgctga aaaaccagct cgagtggcat ctggcggaca actcgcccgc
     1441 cccagctcgc cggaaccgga ataaatttag ccgctggcac ttgcgagcgg cgagcgctgc
     1501 tgggcgctgc tttggcaggg cgatcgctgc gcagaatctc gtgttcagcc tcttgaaaga
     1561 gtttatccat gacggctggt tttttctgcg gatcggccaa tccatccatg acagccaggc
     1621 cttcgaaggc gtccttacaa taaaagactc ctggatcata gcggtttcga tcctcggtgt
     1681 acaggattcg ccaaccgaag cggcggttga ttgccgcgcc gcccaccagg accgggaact
     1741 tgagaccacg gcgctggagc tcattgacga tcagcggcat ttgtttgctg gtgctcacca
     1801 acagcgcgct caggccaatg gcatccgcct tgacttcgac agccttttga ataatcgtct
     1861 cagccggcac ctgtttgcca aggtcgtgaa cggtatatcc attgttggcc aggatggttt
     1921 taaccaggtt tttaccgata tcatgcacat caccgtaaac cgttgccagg acaatcacac
     1981 ctttgctggt gccttccagg cgctccaggt agcgctctac atgcgccacg gattttttca
     2041 tcacctctgc cgactgcagc acgaagggca gaatgagctc cccggcgcca aacttgtcgc
     2101 cgacttcctt catcgccggt agaaggacct cattcaagat cttgactgcg gccacatgtt
     2161 gggtcagacg ctcttcaggg gggagtgcgg cattttctgc cagaatggca tcgatatcca
     2221 actccacacc gtctttatgc cggtgaagga tccgccactg caagcgtccg gcaggcgaca
     2281 gctctgccag gggatcggct ttgccggatg gatcctggac tacaggaccg gcagcttcaa
     2341 aatactcaat caagcgttgt aaagcatctg ggcggcgatt aaagatcaaa tcctctgcca
     2401 tttccctctc cgcctggggg atatctgcat aaggagtgat ttgagctgga tttacgattg
     2461 ccatatccag gccagcctgt acggcatggt agagcattat gctgttcaaa accgggcgcg
     2521 ctgctggcga gagaccaaaa gatacattac tcacccccag cgaggtcagc acacctggca
     2581 gggaagcttt tatcgcccgg attccttcaa gggtgtcgat tgctgaatta ccgaattccg
     2641 gatcgccagt agccagggta aaagtcaggg tatcaaatac caggtcttga ggacgcagtc
     2701 ctatcgtatc gaccgccatc tgataaatac gctgcgcaac ctccagcttg cacctggcag
     2761 ttttcgccat cccctgctca tcgatggtca gcaccaatac cgctgcatta tatgtccgcg
     2821 ccagcccgaa gattcgctct gctttcgcag gtcctgcttc cagatgggtg gaattcaata
     2881 agcagcgtcc tggagctgcc tgcaaggcag cttccatgac ctccggctcg gtgctgtcaa
     2941 tgaccagcgg cacctccacg cccagggcca atttcttcac cacctggcgc atttgctgca
     3001 cttcatccgt ccgctcggta actgcgactg agatatccag ggcatgagct cccccgttca
     3061 cctgctcacg cccgatttcg agaatggcat cataatcctc ttccagcagc agacgcttga
     3121 atttacggct gccctgggcg ttacagcgct ccccgatcag aagcggagga ggatcctgtt
     3181 gcatcggggc agcctgtgtt gctgaggcca gaaacggttc gctcagcaca ggccggacag
     3241 gcgctgagac tcgtcccgca ccttgcccgc ccaggcgatc cactagcagc ttcaaatggg
     3301 caggggtcgt tccgcagcaa ccgccaacaa cattgatgtg atatttttcg acaaattcgg
     3361 tcagcgcctc agcaaaaggc tccggctcca ggggatatac cgcttcacca tccacattca
     3421 acggcagacc cgcatttgga atacacgata ccggtagacg ggaatgctcc ccgagatagt
     3481 tgatcggagc gcgcatgtgt tcaggacctg tcgagcagtt caaaccgagc acatccaccg
     3541 gcatcccctc caggattgcc agggccgcgc cgatatcggt gcccaaaagc attctccccg
     3601 tagtatccag ggtcacctga gcctgtacag gcaagcgcat acccgtggcc gtaaatgctt
     3661 tttgtaaacc ggtcagggct gctttaacct ctaaaatatc ttgcgatgtc tctatcagaa
     3721 gtaaatcagc gccgccctca atcagcccaa ccgcttgctg gcagaaaata tccgccagtt
     3781 catcaaaagt aacatttgcc agatcaggat catcggcaga gggaagtttt cccgatggac
     3841 caatcgagcc tgcgacgaag cgcggctggg tgggcgtgga atattcatct gccagccgcc
     3901 tggccaggct ggctgcggca aagttgattt cgtagacctt atctgccagg ccgtactccc
     3961 ccagggtcaa ccggttggcc cgaaaggtat ccgtctcaat cacatcgaca cccacatcca
     4021 gaaaagagcg atgaacctgt tcaaccatct cgggtgcagt cagtaccagg acatcattac
     4081 acccattcaa atcttctccg ccaaaatgct ccggcgtgag ttttaaacct tgtaagctgg
     4141 tgcccatggc gccatcaaaa atcacgaccc gctgttcgac tgcatccaga tagctccgtt
     4201 taattttcgg ttcaggcatg aaatccaatc tccaatcaac aaaacgcctc tccagaagag
     4261 aggcgtcaag gtcagcatga cctcatcttc cagttcatcc agcttggtct ggatggctga
     4321 cggaattggc acccggtgac ttagcacgac caggttgccg aggtttcgca gggccgttcc
     4381 ctccacctct ctcgatgagt gaaaccagag cttgtcaaga aactttgtct gattttcgga
     4441 aagagtttac cacacgccgc aaggcttgtc aaagcgcata gctcgcttta cgcggcatac
     4501 gggacatgat aaacttgttt tgaatgttaa gttttattct aatggatttc aatggaacct
     4561 attcgccgca tcacaaagta tgatcatcta aaaatcctgg ccgattcgcg ccgcctgtat
     4621 atattgcagc gcctgatggc tggtccggca acgctttcga tgttggggaa agaattgggc
     4681 gagcaccctg cccgtgttcg ccatcacctc aaaaaacttg aagaaatcgg attggtggag
     4741 ctggtaaata ctcaggttgt acgcggtttt attgaaaaat actatcgcgc caaagcccgg
     4801 gcttttttat tgaatgatct gatcttgcct gccgagactc cgcccgacca ccagaccctg
     4861 ttcattatgg gcagtcacga cctggcgctc gaagcgctta ttcaacatac ccaggaaaag
     4921 gctcatcaga ccagttatct gagcatccct actggaagtc tggaaggtct ggtcgccctg
     4981 cgccagggga cggcgcattt tagtggggct catctgtggg atgctgaacg aaatgaatac
     5041 aacctcagct atgtacatta tttctttcca gaccaggata tggtgctgct caccctcgcc
     5101 caccgtgagc aaggcctgat ggtccgccct ggaaatccac gccagatcaa aaatctggtg
     5161 gacctggtac gcccagatat catcctgatt aaccgcaatc gcggttccgg gacgcgcctg
     5221 tggctcgacc atcagtttca ccagctgggg atcgccggcg cgcagttaca gatcttgcaa
     5281 ggcgacgcac gcacgcatac tgcctctgcg gaagctgtgg gatccggact ggctgatgtc
     5341 gggcttggag tgcatgctgc tgcccaaaaa gctggcctgg attttattcc tctgtttcaa
     5401 gaacgatttg atctggtgat gccggcggcc ttattaaact caccggccat gcagccgctg
     5461 ctcaacgatt tacaaagcgc cgaaatacgc cagatcatcc gggatctgga aggttatgac
     5521 tctaccgaaa ccggtaaaca aataactccc cagcccggtt aaggatgggg agccatataa
     5581 ctcatttttc cagccaaaaa tttagctcat ctcttgccgt ccagacaggg cccgtagcag
     5641 cgtgctctgg tcggcgtatt ccaggtcgcc gccaaccggt aagccgcgcg ccaggcgggt
     5701 caggcggaca ccactggctg ccagctgctg ctgcagataa gcggccgtat aatcaccttc
     5761 catgctgggg ttggtcgcca ggataatttc gacaatccct ccggcgcgca cgcgctccaa
     5821 cagctcatgg attttcaagt cgtctggacc aatcccttcg atgggcgaga gcgcgccatg
     5881 taagacatga taccgtcccg ggtaagccgc agtccgttct aaagccagca catccagagg
     5941 ctcttctacg acgcaaatca tcctggaatc gcggtgggca tccccgcaga tctcgcactc
     6001 ctggcgacct gccatagtga tattaaaaca aatcgggcag taggtcgtac tggtcttcag
     6061 cgcactcaaa gcctgtgcca gatcagtcgg tagatcatcc gaggtgcgca gtaaatgaaa
     6121 cgtcagccgg gacgccgttt tcgggccaat cccaggcagg cggctgaaag cattgattaa
     6181 attctggatg ggttctggaa gcaacatcag gcagttaaac agacatttgc agattaacta
     6241 gaaaggcaat ccgcccccgg ccagcggtcc aagccgctcg gcagccatcg cgcgggactt
     6301 atccagggca gagttaaaag cggtcaggac catgtcctgg agcagctcta catcgccatc
     6361 tttcaggatt tctggatcga tctcgacggc atgacagtgc tggtcgccgg taacactcac
     6421 cttgatcgcc ccgccacctg cggtagcgct gacgatttcc gtggctaatt gagcctgggt
     6481 cgcctggatt tgctcctgaa gtttttgtaa ctgttggacc atacccatct gattgggcac
     6541 cttgggacct ttaaatcctt ttgccatgct gactcactcc tgttcgggcg atttcatatt
     6601 gattaaaatt attgcacatc caccagctca ccacccaaat cccgcatcgc tgcggcaacc
     6661 atgccatcgc tttctacatc aggggggagc gagccgccgc gacctgtgac cacaaaacag
     6721 cgcaggggaa tatcactccc caatacctgc tggatcacct ggcgagcgag atcgacatgc
     6781 tcgccttttt ccattttggt tttcgcaaat tccccattaa accccaaaaa cagaaccccg
     6841 tctttcattc caaacggctt gcacgaattg agcaaggcct gggtctgctt gctttgctgg
     6901 ccaatggact ggaggattcg ccgccagtgc tcagtcacac gtttaaatgt caggtcgtcg
     6961 agcggctgtc cttctgttgg agtgggcgaa ggggctgcct ccaccgcggc gggggaggtt
     7021 tgcgccggag cttccgcctc aggccgggtt ttccgaggtg tcagggtaga tctgggagct
     7081 gatgcctggt tgttgagatc agattcaggc tcgccggcag gctctaaggc ttcgataaac
     7141 gccagctcca gaggcagcga gggttgccag gcattcttac tttcattggc agcggcatta
     7201 aataagcgga tcaggcgcag cagctccgct ggtgtgtatg cctgagcgtg acgggccatg
     7261 atggcgcgcg tctcagtggt cgcatcaatc tgatctgcat tgcccattcg catgagcagt
     7321 aaatccctta gataatccac aaattggcgg gcatattggc gtgggtcact cccactatcc
     7381 agggccagct ggagggtgtt gatgcctgta tctggctgcc tggcaatcag ggcttcaatt
     7441 acatccagga cggcctggct ggctgctgtc ccgagcaggt tctgagtcaa ttcaagtgta
     7501 attgtctgcc cggtggaggc cagctgatcc aacagggaaa tggcatcgcg cagcgcgcca
     7561 gtcgactggc gggcgatgag ggtcagagca tcattctcga cgggaatatt ttcttgctcg
     7621 gcttgtttgc gcaggtgagc cacaatatca gccacgggga tacgccggaa ttcgtggcgt
     7681 tggcagcgcg aaaggacggt ggcagggatc ttgtggattt ccgttgttgc caggataaag
     7741 atagcatgcg agggaggttc ttccagggtt ttcagcagcg cattgaaagc tgccgttgag
     7801 agcatgtgga cttcatcgat gatatatact ttatatcgac cctggttggg ggaaaaattg
     7861 atcttctcgc gcaaatcccg gacatcatca acactggtat tcgaagcggc atcgatttca
     7921 atcaaatcca gaaatcgacc ttctgttact gcctggcagt gagcacaaac cccacagggg
     7981 cggtctgcca ggccttcagc tagacagttc acagcctttg ccagcagccg ggcggtcgtg
     8041 gtcttgccag taccgcgcgg cccagcaaat agataagcat ggccgatgcg ctcggcgatt
     8101 aacgcattgt gcagggtttg gattacgtgt tcttgcccca ccacttcatt ccagttttgg
     8161 ggccgccatt tacggtataa agcttgagcc atggttagag tctatcagag aggggcgtag
     8221 aggtcaaccg ctcgatctcg cggtccagct ggatagctgg cagctgaaaa ttctccaaag
     8281 gtgcctgtaa attgtaatcc cggatctgct ggttgagccg ctgcacctgg gttgtaaaga
     8341 cctccacagc cagctgccac tcagattcga tct
//
LOCUS       DATMXK010000416         3782 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_169546, whole genome shotgun sequence.
ACCESSION   DATMXK010000416 DATMXK010000000
VERSION     DATMXK010000416.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3782)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3782)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3782
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169546"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..948)
                     /locus_tag="VN363_07700"
     CDS             complement(<1..948)
                     /locus_tag="VN363_07700"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ84433.1"
                     /translation="MALTLSAQHLVKRYGGVVALSDGNLEINTGEVVALIGANGSGKS
                     TLSKVIDGVVTLDGGQILLDGNPVNFSSPQAAKKMGIATVFQELSLIPQMSVADNIWL
                     TREPLGLGGVVNRKDVFAQTKELLSLFEGTFKTELRPEVTINELPPDEKQIVEVLKAI
                     SFNPRILILDEATASLDSRQVNRLFELVAKWKEEGRAIVFVSHRMEEIFRIADRYSVL
                     RNGKTVGAGNMAEVTEKDLVKLMIDKDSVFSYSRTGKQITENPVCLEVKDLHSRVLHG
                     VSFDVRESELVGLGGLQGQGQRDLLLSLFGDIPFTGNIQY"
     gene            complement(1028..2194)
                     /locus_tag="VN363_07705"
     CDS             complement(1028..2194)
                     /locus_tag="VN363_07705"
                     /inference="COORDINATES: protein motif:HMM:NF024799.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWQ84434.1"
                     /translation="MSKKTVMLLVTLITIFSTVLAACSSATPAPTEEPAPVTTEAPAP
                     VATEAPAEPVATEAPAEPVDDTKFTIGVSNPFISSEYRTQMIAELIETNQEYMDAGVT
                     NELVIESADTDVAGQIQQLQNLMAKGVDAILVNPGDVNGLNATLEEAVAKGILVISVD
                     QELNVPGVYNVGIDQKLFATTSAEWLAEKLGGKGDVVTIEGFPGHPANVVRMEGVAEV
                     FAKYPDIKVLATDTGKWDEATGQQVMSNFLAAYPNLTGYWTQDGMAIGALQAVMAANP
                     AVWPQGVGEGRCQYLKLWQEALTMKPEFDSIAVSNHPGVSPTGLRIAVNILQGKEVDT
                     AKLGGANGLSFVLPLVEVITSENLADGLAMCEGKPDAFLLDDILTEEEVQQYFK"
     gene            complement(2329..3588)
                     /locus_tag="VN363_07710"
     CDS             complement(2329..3588)
                     /locus_tag="VN363_07710"
                     /inference="COORDINATES: protein motif:HMM:NF012692.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ROK family transcriptional regulator"
                     /protein_id="HWQ84435.1"
                     /translation="MVRPDQVFAQQARPNVASLSMNGAEAKIFQALRQQGEISRTDIS
                     NLTGWSKAKTSHKIREFVEKGLLVEVGEGASQGGRRPRLLQINVQLGYLVGMDIGATS
                     LDIALADVSGKICQRRTAVADVRQPPEEYLSRSRELILEMAAAQGCIPGQILGIGIGV
                     PGPVDFGRGVLVAPPLMPDWEHFLISAFLQPAFPNAFIVVDNDVNIMALGEQRAGDGI
                     GIDHFIFVKIGTGIGSGIISNGSIHRGSIGSAGDIGHICVDKQGPICRCGNTGCLEAM
                     AAGPAISEKAVYAATHGASPLLRQMMDANGGWLRPEDVNLACREGDQAALDIIRASGE
                     MIGDVLAGLVNFFNPSHIFIGGGITSFGNHLLVAIRRAVLRRSLPLATTHLVINFSRM
                     GPDIGVIGAIALVSEYVFMPGGQFYRS"
BASE COUNT          872 a         1110 c          919 g          881 t
ORIGIN      
        1 gtattgaata ttgccggtaa acggtatatc cccaaacaaa gaaagcagca aatctcgctg
       61 cccttgaccc tgcaaaccac ccaacccgac caactcactc tcgcgcacat cgaaacttac
      121 cccgtgtaaa acacggctgt gcaggtcttt gacttcgaga caaaccgggt tttcagtgat
      181 ttgcttgccc gtgcgagaat aactgaacac cgaatccttg tcgatcatca acttaaccaa
      241 atccttctcg gtaacttcgg ccatattccc ggcgcctaca gttttgccat tgcgcagcac
      301 actgtaacga tctgcaatgc ggaaaatctc ttccatgcgg tgagaaacga atacaatcgc
      361 ccgtccttct tccttccact tagctaccag ctcaaagagg cgattcacct ggcgactatc
      421 caggctggct gtggcttcat ccaaaatcag gatgcgagga ttgaagctaa tggctttaag
      481 tacctcaacg atttgttttt catccggggg cagttcattg atcgtcactt ccggtcgtaa
      541 ttcagtctta aatgtgcctt caaacaacga tagcagctct ttggtttggg cgaaaacatc
      601 cttgcggttg acgacgcctc ccaggccaag tggctcgcgt gtcagccaga tattatcggc
      661 aaccgacatc tgtgggatca ggcttaattc ctggaagacg gtcgcaattc ccattttctt
      721 ggctgcctgt ggagatgaaa aatttaccgg attgccgtcc aggagtatct gcccaccatc
      781 cagggtgaca acaccatcga taactttgct cagcgtgctc ttgccgctgc cattggcgcc
      841 gattaatgcg acaacttcac cggtgttgat ttccaggttg ccatccgaaa gggcgaccac
      901 acctccatat cgtttaacga gatgttgagc agaaagcgtc agggccataa atctccctta
      961 ttgaaaacca ttagaaaggt tgcgagggat ggtctcatcc ctcgcaacca gatggtgaag
     1021 aatagactta cttgaaatac tgctggactt cttcttcggt cagaatatcg tcaagcagga
     1081 aagcatcggg cttgccttcg cacattgcca gaccgtctgc caggttctcc gaggtgatca
     1141 cctcaaccaa aggcagcacg aaggacaatc cattcgcccc acccagcttg gcggtatcca
     1201 cttctttgcc ctgcaggata ttgaccgcaa tccgcagccc ggtcggggag acacccgggt
     1261 ggttggatac ggcgatgctg tcgaattcgg gtttcatcgt cagcgcttcc tgccacagct
     1321 tcagatactg gcagcgacct tcacccacac cctgcggcca gactgccggg ttggctgcca
     1381 tcaccgcctg cagtgcccca atcgccatcc cgtcctgcgt ccagtaaccg gtcaggttgg
     1441 ggtaagcagc caggaaattg gacatgacct gctgcccggt ggcttcatcc catttgccgg
     1501 tatccgtcgc caataccttg atgtccggat acttggcgaa tacttccgcc acgccttcca
     1561 tgcggactac attcgccggg tgtcccggga agccttcgat cgtgaccaca tcccccttgc
     1621 cacccagctt ctccgccaac cattcagccg aggtcgtcgc aaacagcttc tggtcgatgc
     1681 ccacattgta cacgcctggc acattcagct cctggtctac ggagatcacc aggatgccct
     1741 tcgccacagc ttcttccagg gtcgcattca agccgttcac atccccagga ttcaccagga
     1801 tcgcatccac acccttcgcc atcaggttct ggagctgctg gatctgcccg gcgacatccg
     1861 tgtcggcgct ctcgatcacc agctcatttg tgaccccggc gtccatatat tcctggttgg
     1921 tttcaatcag ctcggcgatc atctgcgtgc ggtattcgct gctgatgaat ggattcgata
     1981 caccaatcgt gaacttggtg tcatcaaccg gttctgccgg agcctcggtt gctacaggct
     2041 cagctggtgc ctccgtggct acaggagcag gcgcttcggt tgttacagga gccggttctt
     2101 ccgtcggagc aggcgtggcg ctgctgcagg cggctaaaac ggttgaaaag atggtgatca
     2161 gggtcaccaa aagcattacg gttttcttag acataaggtt cttctcctac ataagtgttt
     2221 atggttggtc tatattcgag ctaacgcagg ttatttcagg ctaatattca cctcctcagc
     2281 gagcttagcc gcaccttcat gggcggattc aatggcaatc tactccgatt atgatcggta
     2341 aaattggccc cctggcataa acacatattc gctaaccagg gcaattgcac cgattacacc
     2401 aatatccggg cccattcgtg aaaaattgat caccaggtgg gtcgtggcga gggggagcga
     2461 gcgccttaaa actgcccggc ggatggcaac cagcagatgg ttgccgaagc tcgtaatccc
     2521 gccgccgata aagatgtgac tgggattgaa gaaattcacc agtccggcca gcacgtcccc
     2581 gatcatctcc ccgctggcgc ggataatatc cagggcagcc tggtcacctt cgcggcatgc
     2641 cagattgaca tcctcagggc gcagccagcc cccattggca tccatcatct ggcgcagaag
     2701 cgggctggcg ccatgagtcg cagcatatac tgccttctcg ctgattgccg gaccggccgc
     2761 catcgcctcc aggcagcccg tattcccgca gcggcaaatc gggccctgtt tatccacgca
     2821 gatatggccg atatccccgg cggagccgat gctgccgcga tggatactgc catttgagat
     2881 aatccccgag ccgatcccgg tgccaatttt gacaaaaata aaatggtcga tcccgatccc
     2941 atcaccggcg cgctgctcac ccaaagccat aatgttgaca tcattatcga ccacgatgaa
     3001 agcattcgga aaggctggct gaagaaaggc gctgatgaga aagtgttccc aatctggcat
     3061 cagcggcggt gcgaccaata cgccgcgccc aaaatcaacc ggccccggca caccgatccc
     3121 gatgcctaaa atctggccag gaatacagcc ttgcgcagcg gccatttcaa ggatcaattc
     3181 tctgctgcgg ctcagatatt cttcaggagg ctggcggaca tccgccacag ccgtacgccg
     3241 ctggcaaatc tttccgctta catccgcgag ggcgatatcc aggcttgtcg cgccgatatc
     3301 catccctacc agataaccca actgaacatt aatctgaagc aagcgcggcc tgcgcccacc
     3361 ctgcgaggcg ccttcgccga cctctacaag gaggcctttt tcaacgaatt cacggatctt
     3421 atgggaggtt ttggcttttg accagccagt gagattggaa atatctgtac gggaaatttc
     3481 accctgctgg cgcagggctt gaaaaatctt cgcttcagcc ccgttcatcg aaagcgaggc
     3541 gacatttgga cgtgcttgtt gtgcaaaaac ttgatcagga cgaaccattc agctgcatcc
     3601 tcaattcgtt ttgtgcaaga atagacaagc acctacggta tagttatatc aaatgagctt
     3661 cgttaattca atatccgggt ttgactaaaa cggcgtaact ttgtttatta ttataacgaa
     3721 gttccgccat tcctaaattc tccacccgaa taaacggaca ggttgagcca gacctggttt
     3781 tc
//
LOCUS       DATMXK010000417         2248 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_139713, whole genome shotgun sequence.
ACCESSION   DATMXK010000417 DATMXK010000000
VERSION     DATMXK010000417.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2248)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2248)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2248
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_139713"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            100..1236
                     /locus_tag="VN363_07715"
     CDS             100..1236
                     /locus_tag="VN363_07715"
                     /inference="COORDINATES: protein motif:HMM:NF015764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="major facilitator superfamily domain-containing
                     protein 6"
                     /protein_id="HWQ84436.1"
                     /translation="MFPLSNGTNFGRLTLFYQSQGFSGTQIGLLSGITPLVTMLFAPF
                     WTGLADATGRHRLILSLTLLGAAAAISIFASFTSFTAILLLAVLYAAFTAPANAFADS
                     ATMLMLKDKKEKYGQVRVGGTLGFGISALLAGVLVESFGLKLAFWGCAILLLSSLAVS
                     QKLVHNPHRASQQAGADFRLLLRNPRWLLFLAMAFAGGIAIAGNNTYFFPYLKELGAP
                     ESLMGLALTIGTLTEIPVLFFGHHLLRRFKPYGLLILAMVVTGARLLLFAAANTPEQL
                     LVVQLLNGLTFPAMWVAGVAYVDEIAPPELSATAQGMFGAMVFGFGAAVGGFIGGPLL
                     ETSGGRGIYLTFGIIVVVVVALAALGMRLLPKKAHFVPASMDSE"
     gene            complement(1282..>2248)
                     /locus_tag="VN363_07720"
     CDS             complement(1282..>2248)
                     /locus_tag="VN363_07720"
                     /inference="COORDINATES: protein motif:HMM:NF024637.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HWQ84437.1"
                     /translation="VLGAYEAPPAAPSSAGPEAESEAPFDEQPAAMSVPGATPAPIPV
                     PTPPAAPAPKPAQASTAGSPAASPAGKMTICPRCGTQNPNLALRCSNCGAPLNVETVK
                     PTAPAVPTARAPLPKWLIIAGALLLVATCVFIFSLIQRGQQTNEVRGVVQSVRWERII
                     PIEALVDVEHQAWQDEIPGGVTLGMCREQVRYESAQPEPNSEEVCGTPYTVDQGSGYA
                     EVVQDCVYRVYDDYCSYTQPEWRVVDSVSVSGADLSPRWPQVSLAQGQRQGEQLQENY
                     TIVFMVDGEQYSYPVSSLEAFDDFQPGSEWNLVVNGFGDLVGVER"
BASE COUNT          365 a          733 c          669 g          481 t
ORIGIN      
        1 agatatcaca gagcagtggc tcgaagaata caatgctatc cggccgcatg aggcattaca
       61 gggtttatcg ccctatcagt atgcggcaga acatacctga tttttccact ctcaaatggt
      121 actaattttg ggaggttgac actcttttac cagagtcagg gtttcagtgg gacgcagatc
      181 ggtttactct ccgggatcac cccccttgtc accatgctct tcgcgccctt ttggaccggg
      241 ctggcggatg ccaccggccg ccaccgcctg atcttgagcc tgaccctgct gggagcggcg
      301 gcggcgatct cgatcttcgc ctcgttcacc tcatttaccg ccatcctgct cctggccgtt
      361 ttatatgccg ccttcaccgc cccggccaac gccttcgccg atagcgcaac gatgctgatg
      421 ctaaaggaca aaaaggaaaa atatggccag gtgcgggtcg gggggacgct cggctttggg
      481 atcagtgcct tactcgccgg ggtgctggtc gaatcgtttg gtctcaaact tgccttctgg
      541 ggctgcgcca tcctgctcct gtccagcctg gcggtcagcc agaaactggt ccacaacccg
      601 catcgcgcca gccagcaggc cggagctgac ttccgcctgc tgctgagaaa tccgcgctgg
      661 ctgctgtttt tagccatggc gttcgccggc ggcatcgcca tcgccgggaa caacacctac
      721 tttttcccct atctcaaaga gcttggcgcc ccggaatcgc tcatgggcct ggcgctcacc
      781 atcggcaccc tcaccgagat cccggtcttg ttcttcgggc accatctcct gcggcgcttc
      841 aaaccttatg gcttgctgat actggcgatg gtggtgaccg gcgcccggct gctgcttttc
      901 gctgccgcca ataccccgga gcagctcctg gtcgtccagc ttttgaatgg gcttaccttc
      961 cccgccatgt gggtagccgg ggtcgcctac gtggacgaga tcgccccacc cgagctgagc
     1021 gccactgccc agggcatgtt cggggcgatg gtgttcggtt ttggcgccgc ggttggcgga
     1081 ttcatcggtg gccctctgct ggagacctcc ggcgggcgcg ggatttatct tacattcggg
     1141 atcatcgtcg tggtggtggt tgctctggca gccctgggca tgcgcctgct gccaaaaaag
     1201 gcgcattttg tgcctgcttc gatggactct gaatgaaatt atgaatggcg ctggcacagc
     1261 cagtgccatt cattgataaa attacctctc cacccccacc agatctccaa acccattcac
     1321 caccagattc cactcgctgc ccggctggaa gtcgtcgaag gcttccaggc tgctcaccgg
     1381 gtagctgtac tgctccccat ccaccatgaa tacaatcgtg tagttctcct gcagctgctc
     1441 gccctggcgc tgcccctgcg ccagcgaaac ctgcggccag cgcgggctca ggtccgcccc
     1501 cgaaacgctg accgaatcga ccacccgcca ctccggctgg gtgtagctgc agtaatcgtc
     1561 gtagacccgg tacacgcagt cctggaccac ctcggcgtag ccgctgcctt gatccacggt
     1621 gtagggcgtg ccgcaaactt cctccgagtt cggctcgggc tgcgctgatt cgtagcgcac
     1681 ctgctcgcgg cacataccca gggtcacccc gccggggatc tcatcctgcc aggcctggtg
     1741 ttccacatcg accagggcct caatcgggat gatccgctcc cagcgcacac tctgcaccac
     1801 accccgtacc tcgttagtct gctggccgcg ctggatgagg gagaagatga aaacacaggt
     1861 tgccaccagc agcagggctc cggcgatgat cagccatttt ggcagggggg cgcgggccgt
     1921 tgggacggct ggggcggtgg gttttaccgt ctccacgttc agcggcgccc cgcagttgct
     1981 gcagcgcagc gccaggttgg gattctgcgt cccacagcgc gggcagatcg tcattttccc
     2041 ggctgggctc gccgccggac tccccgcggt cgaggcctgg gcaggtttgg gcgccggggc
     2101 cgctggaggt gtgggtaccg gtataggggc aggtgtcgcg cctggcacgc tcatcgccgc
     2161 gggttgttcg tcgaatggag cctcgctttc ggcttctggc ccggctgagg atggcgcagc
     2221 tggcggggct tcgtacgcgc ccagcacc
//
LOCUS       DATMXK010000418         2408 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_140488, whole genome shotgun sequence.
ACCESSION   DATMXK010000418 DATMXK010000000
VERSION     DATMXK010000418.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2408)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2408)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2408
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_140488"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2408)
                     /locus_tag="VN363_07725"
     CDS             complement(<1..>2408)
                     /locus_tag="VN363_07725"
                     /inference="COORDINATES: protein motif:HMM:NF024603.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWQ84438.1"
                     /translation="AALDEGAPLPPVVLGYTPAGGQEMPLDGAIEVEFDQSMDPGSTA
                     NAWQVSGPDGEQVQGDLAWQDGRRLRFTPSESWETGAVYRASLGITALSAAGEPLRDP
                     LTLEFNTIGELQVSQVFPAHSAVDVAGDAVITVIFNRPVIPLTIVEERSSLPQPLVIS
                     PETPGSGEWVNTSVYNFYPTRQLKGGVTYSVAVQAGLTDATGSSSLAEEFTWEFSTAV
                     PSLASYELGSGEVNPPYARGNVRLDEFFRIYFRQPMDAASSEAALSLTSLNGEPVPVQ
                     TTWSEDAQTLVITPTQNLALDTTYDLILTEAALAEDGGALNAGLSYRFTTIPAPAVLS
                     SKPANNARQGNYTNYFSIQFASPMNIETLKRRITILPAPEEDVQWWFNDWDQTYAGSF
                     LRPSTQYTIRLLPGMEDIYGNAIARGSEIRFTTAAMQPEAALQMPYQPAILRAGGPQE
                     FYLTYRNVASVSLQLYRLTPELFVQLENGTRSRYDYQPPADDLVWEMEQVSKARLNER
                     SLELIDLTSEGNEALETGFYFLTLDAPVAYKSGLYVDSRLILVATANLAFKSAADEEL
                     VWLTDLETGQPLPGVPIIAYNNQFKEIGRGETAADGSLQLSNLPVPKDPYTPRFVMTD
                     PGLASETLGFASSYWNSGVSLWDYGVWGSYYAPPNQPTAYVYTERPIYRPGQPVYFKG
                     IVRLDDDLDYSIPDAARVEVIIDSFEDEVYRESVTLSAFGSFDGQWVIDTDAALGAYN
                     LRVRFPGQTAESGDIGSVSFNVAEYRRPEFQVNVSAQAPAVLAGEDMQFSTQADYYSG
                     GGVA"
BASE COUNT          493 a          730 c          694 g          491 t
ORIGIN      
        1 ggcgacccca ccgccagaat aataatccgc ctgcgtggaa aactgcatgt cttcccccgc
       61 caggactgcc ggagcctggg cactgacatt tacctggaat tctgggcggc ggtactcggc
      121 tacattaaag ctcacactcc cgatatcccc gctctccgcg gtctggccgg ggaaacgcac
      181 ccgcagattg taggctccca gggcggcatc ggtatcgatc acccactgcc cgtcaaagct
      241 gccaaacgca gagagggtta cgctttcccg atagacctca tcctcaaaac tatcgatgat
      301 cacctctact cgggctgcgt cgggtataga ataatccagg tcatcatcca ggcgcacgat
      361 gcccttgaag tacaccggct gccccgggcg gtaaatcggg cgctcggtat acacatacgc
      421 cgtgggctgg ttgggcgggg cgtaataact gccccacacc ccataatccc acagggatac
      481 gccgctgttc cagtagctcg aagcaaatcc cagggtctcg ctcgccagcc cgggatccgt
      541 catcacaaag cgcggtgtat agggatcctt gggcactggc agattgctga gctgcaaact
      601 gccgtccgcg gcggtttcac ccctcccaat ttccttaaac tgattattgt aggcgatgat
      661 gggaacgcct ggaagcggtt ggccggtttc cagatccgtg agccagacca gctcttcatc
      721 cgcagctgat ttaaatgcca ggttggcggt ggcgaccagg atcaaacgcg agtcgacgta
      781 aagtccactt ttatatgcga ccggagcatc cagagtcaga aaataaaaac ccgtctctaa
      841 ggcttcattg ccctcgctgg tgagatcgat caattcgagg ctgcgctcat tcaggcgcgc
      901 cttgctcacc tgctccatct cccagaccag gtcatccgca ggcggctggt aatcatagcg
      961 cgagcgggtg ccgttctcca gctgcacgaa cagctccggc gtcaggcggt acagctgcag
     1021 gctgactgag gcaacattgc ggtaagtgag gtaaaactcc tgcggcccgc cggcgcgtaa
     1081 aatagccggc tggtagggca tctgcagagc agcttctggc tgcatggcag ccgtcgtgaa
     1141 gcgaatttca ctccccctcg caatcgcatt gccgtagata tcctccatgc cgggcagcag
     1201 acgaatggtg tactgcgtcg aagggcgcag gaagctcccc gcataggtct ggtcccaatc
     1261 gttgaaccac cactggacat cctcttccgg ggctggcagg atggtaatac gccgtttcag
     1321 tgtctcgata ttcatgggcg aggcaaactg gatggagaaa tagttggtgt aattcccctg
     1381 gcgtgcgtta ttggctggtt tgctcgagag caccgccgga gcagggatcg tcgtaaagcg
     1441 ataactcaag cctgcgttca acgccccgcc atcttcagcg agggccgctt cggtgaggat
     1501 caggtcatag gttgtatcca gcgccaggtt ttgcgtgggt gtgattacca gcgtctgggc
     1561 gtcttcgctc caggtcgttt gcacaggcac cggctccccg ttaaggctgg tcaggctcag
     1621 agctgcttcg ctgctggcgg catccatcgg ctggcgaaaa taaatgcgga agaattcatc
     1681 caggcggaca tttcccctgg catagggtgg gttgacttca ccgctgccca actcgtagga
     1741 agccaggctg ggcaccgcag tgctgaactc ccaggtaaat tcttctgcca gactggagga
     1801 gccggtcgca tccgtaaggc cggcctggac ggctacacta taggttacgc cgcctttaag
     1861 ctggcgggtg gggtaaaaat tatagaccga cgtgttgacc cattcgccgc ttcccggtgt
     1921 ttcgggcgaa attaccagcg gctgaggcag gctgctgcgc tcttcgacga tcgtcagcgg
     1981 gatcaccggg cggttaaaga tcaccgtgat caccgcatcc ccggcgacat ccaccgcgga
     2041 atgagcgggg aaaacctggc tgacctgcag ctcgcctatt gtattgaatt caagggttag
     2101 gggatcgcgc agcggctcgc cagcggcgct gagcgccgta attcccaggc tggcgcgata
     2161 caccgccccg gtttcccagc tttccgaagg cgtaaaccgc aatctacgcc catcctgcca
     2221 ggccaggtca ccctgcactt gctcaccatc cggcccggag acctgccagg cattggccgt
     2281 cgacccggga tccatcgact ggtcgaattc cacttcgatc gcgccatcca ggggcatttc
     2341 ctgcccgccg gccggggtat agcccaaaac gactggtggg agtggtgccc cttcatctaa
     2401 agctgcaa
//
LOCUS       DATMXK010000419         2237 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_152531, whole genome shotgun sequence.
ACCESSION   DATMXK010000419 DATMXK010000000
VERSION     DATMXK010000419.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2237)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2237)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2237
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152531"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(6..1259)
                     /locus_tag="VN363_07730"
     CDS             complement(6..1259)
                     /locus_tag="VN363_07730"
                     /inference="COORDINATES: protein motif:HMM:NF013347.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent RNA methyltransferase"
                     /protein_id="HWQ84439.1"
                     /translation="MTDPTGKRLPAKQEKPAVQPSLAQYFAVSGPGLERLTAHELHKL
                     GSLPEPPAVADTTQSTGNDLEGGVSFSGDLHELYRANLHLRTASRVLVRLGEFRAVAF
                     YELRKKAARLPWESYLTPGQPVSLRVTCRKSRLYHSDAVAERVVGAIGDRLNRPSRAL
                     AYAEDSMSDEVQLVIVRFVHDICTISIDSSGAGLHRRGYRLAVAKAPLRETLAAGIIL
                     AARWHSEQPLVDPFCGSGTIAIEAALLASARPPGLDRKFAFMNWPGYDPAAWQKLGRE
                     TMFAGPPPQPVILASDRDAGAINMAQENADRAGVAEWIDFKQQAFSAIAPAGRGWVIT
                     NPPYGVRVSTTQDLRNLFAHLGDVLRQHCPGWNYAILSNEDKLIAQLHLPEAGRLWLE
                     NGGLPVKLVMGMVPEINSMSKIHAD"
     gene            complement(1252..1746)
                     /locus_tag="VN363_07735"
     CDS             complement(1252..1746)
                     /locus_tag="VN363_07735"
                     /EC_number="3.1.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF015048.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0047617 - acyl-CoA hydrolase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PaaI family thioesterase"
                     /protein_id="HWQ84440.1"
                     /translation="MNGMAFQDYYPDALNYCYGCGRLNEHGLQIKSFWEGDEAVCYYE
                     PLPYHTALPGYVYGGLIASLIDCHSTGTASAAAYRAAGRAMDTEPPFRFVTAGLHVDY
                     LKPTPLGVMLELRATVKEIKGRKVVISSTVSANGEVCVRGEVICVQAPEALLSGPGEP
                     PAHD"
     gene            1914..2180
                     /locus_tag="VN363_07740"
     CDS             1914..2180
                     /locus_tag="VN363_07740"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84441.1"
                     /translation="MLNIGIYRQCLQETVQEAIDHTDGSNLQISEYLWSMKVSGWFTR
                     NKLEKKKAIEEARAAFDNHRHWPLAIVISHLGLDSKEMRLPDLT"
BASE COUNT          551 a          639 c          564 g          483 t
ORIGIN      
        1 gttcatcaat cggcatggat tttactcata gaatttatct caggcaccat acccatgacc
       61 agcttcaccg gaaggccacc attctccagc cataagcggc cagcttccgg caggtggagc
      121 tgggcaatca gcttatcctc gttggataag atggcgtaat tccagccagg acagtgttgg
      181 cgaagcacat cgcccagatg agcaaaaagg ttgcgcagat cctgggtggt gctaacccgc
      241 actccataag gtggattggt aattacccaa cctcgtcccg caggcgcgat cgctgaaaag
      301 gcttgctgct tgaaatcaat ccactccgca accccggccc gatctgcatt ttcctgagcc
      361 atattaatcg ctcctgcgtc ccgatctgat gccaggatca ccggctgggg aggtgggcca
      421 gcaaacattg tttcgcgacc cagtttctgc caggcagccg gatcataccc tggccaattc
      481 atgaaggcga attttcgatc cagcccggga ggcctggcgc tcgccagcag ggccgcctca
      541 attgcgatcg tgcccgaacc gcagaaggga tcgaccagtg gctgctccga gtgccaccgg
      601 gctgccagga taatccccgc agccagagtt tcgcgcaaag gagcttttgc aactgccagg
      661 cggtaaccgc gccggtgcag acccgctcca gacgaatcaa tgctgatggt gcaaatatca
      721 tggacaaaac gcacaatcac taactgcacc tcgtctgaca ttgaatcttc agcatacgcc
      781 agcgcccggg atggacggtt caggcgatca ccaatcgcac ccacgacacg ctcagcaact
      841 gcatcggaat ggtagaggcg cgatttacgg caggtaactc gcaggctaac cggctggccc
      901 ggggtgagat aactttccca gggcaggcgg gcagctttct tacgcagctc gtaaaatgct
      961 acagcacgaa attctcccag ccggacaagc acacggctgg cagttcgcaa gtgtaaatta
     1021 gcccggtaaa gctcgtgcag atcaccgctg aaactcaccc caccctccag atcgttaccg
     1081 gtggattggg tggtatctgc gacagccggc ggttccggga gggagcccag cttgtgcagc
     1141 tcatgggctg tgagtctttc cagtcccgga ccagacacag caaaatactg cgccagtgaa
     1201 ggctgcactg caggtttttc ctgttttgcc ggcaatcgtt tgccagtggg atcagtcatg
     1261 agcaggcggc tctccaggtc cggacaataa tgcttctgga gcctgtacgc agatcacttc
     1321 acctcggaca cagacctcac cattggcaga tacggtcgaa gagatcacaa ctttgcgacc
     1381 tttaatttct ttgacggtgg cgcgcagctc cagcattacg cccaatggag taggcttgag
     1441 ataatcgaca tgcaaacccg cggtcacgaa ccggaatggc ggctcagtgt ccatggcgcg
     1501 gccggcagcg cggtaagctg cagccgaggc tgtacctgta ctgtgacaat cgatcaaaga
     1561 agcgatcaga ccaccataga cgtagccagg caaggctgtg tggtaaggca gcggctcata
     1621 atagcatact gcttcgtcgc cctcccagaa acttttaatc tgcagaccat gctcattcaa
     1681 acgcccgcag ccataacaat aattcagcgc atctggataa tagtcctgaa aagccattcc
     1741 attcattgat cgatccttct gtcagtaata atctgcggta acgccgttga aaatcaacca
     1801 gcatccagtc cctattatat ccttgtcata cctgcagccc acacttattg atcatagacc
     1861 attatcgata tcgatcctga acagcttaag gtatactcag gatagaggct actatgctga
     1921 atatcgggat atatcgccag tgcttgcagg aaactgtcca ggaggccatt gatcatacgg
     1981 atggctcaaa cctgcagata tctgagtatc tctggtctat gaaggtcagc ggctggttta
     2041 cgcgcaataa attagaaaaa aagaaggcca tagaggaagc ccgcgcagcc tttgataatc
     2101 accggcattg gcctctggcg attgtgatct cccacctggg tctggactcc aaagagatgc
     2161 gtttgccaga tctaacttaa tttcctccct acaaacccat actcgccaat tactcagctc
     2221 ccgtacttat tctactg
//
LOCUS       DATMXK010000420         3527 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_160837, whole genome shotgun sequence.
ACCESSION   DATMXK010000420 DATMXK010000000
VERSION     DATMXK010000420.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3527)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3527)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3527
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_160837"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            110..1207
                     /gene="hypD"
                     /locus_tag="VN363_07745"
     CDS             110..1207
                     /gene="hypD"
                     /locus_tag="VN363_07745"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560192.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016530 - metallochaperone activity
                     [Evidence IEA];
                     GO_process: GO:0036211 - protein modification process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase formation protein HypD"
                     /protein_id="HWQ84442.1"
                     /translation="MKYLDEYRDARLVHKVMDELRRTITRPWVIMEICGGQTHAILRY
                     GLEQLLPPEIELVHGPGCPVCVTSLELIDKALEIASRPGMIFVSYGDMLRVPGSRQDL
                     FSIRSAGGDVRVVYSPLDTVRIARENPDKQVVFFAIGFETTAPANAMSVLQAQTLGLD
                     NFSILVSQVRVPPAIESILSSPHNRVQGFLLAGHVSAVMGYWEYPPLAARYNVPMTVT
                     GFEPLDIAQGILETVRMLEDGTVGVRNAYARLVSERGNQPAQAVINQVFMECDRNWRG
                     IGMIPMSGWRLRPEFASFNAEVRFDVQDINSQESPLCMAGLVLQGLRKPSDCPAFGRQ
                     CTPETPLGATMVSAEGACAAYYRYKITPEGA"
     gene            1210..2262
                     /gene="hypE"
                     /locus_tag="VN363_07750"
     CDS             1210..2262
                     /gene="hypE"
                     /locus_tag="VN363_07750"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560193.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydrogenase expression/formation protein HypE"
                     /protein_id="HWQ84443.1"
                     /translation="MGISEGPSCPLPLPHHDRIILGHGSGGKLTDELISRLFYPCFDN
                     PALRQGNDSAALSLENGARIAVSTDAHVVSPLFFPGGDIGRLAVCGTVNDVAMSGAIP
                     RYLTAGFILEEGLEVSLLGKVIESMRDAAAEAGVQIAAGDTKVVQRGKADGLYITTAG
                     IGILPAGRQIGGAQARPGDVVLLSGPLGDHGIAVLAARGELGFETDIQSDAAPLNHLI
                     AAMLAASPNVHVLRDPTRGGLGTTLNEIAGQSQVGIQIDENAIPVRPAVAAACEMLGF
                     DPLYIANEGKLVAIVAAQDAELILEVMHRTRYGEDAVRIGEVLPAPAGRVLLRSRIGG
                     VRLVERLSGELLPRIC"
     gene            2398..3471
                     /gene="fbaA"
                     /locus_tag="VN363_07755"
     CDS             2398..3471
                     /gene="fbaA"
                     /locus_tag="VN363_07755"
                     /EC_number="4.1.2.13"
                     /inference="COORDINATES: protein motif:HMM:TIGR01520.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004332 - fructose-bisphosphate aldolase
                     activity [Evidence IEA];
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class II fructose-bisphosphate aldolase"
                     /protein_id="HWQ84444.1"
                     /translation="MANLEDLVPAGVIVGEDVQKVFAYAKAHGFALPAANCTGTNTVN
                     AVLETAREMDSPMIIQFSNSGAAFYAGKSLSNDGQRASIAGAIAGANHIHQLAEAYGV
                     RVMVHTDHCAKKLLPWVDGLLEAGEKYYQAHGKPLFSSHMLDLSELSLQENVETCKRY
                     LERMSKIDMTLEFELGVTGGEEDGVDNTGVSSSRLYTQPEEVAYAYEELMKVSPRFTI
                     AAAFGNVHGVYKPGNVKLRPVILQNCQEYIQQKYNTPEKPLNLVFHGGSGSTSEEIRD
                     AVSYGVVKMNIDTDLQWALWDGVKNYYVKNADYLQGQIGNPKGADEPNKKFYDPRTWL
                     RAGEEAFKTRLRAYFEELNTGKM"
BASE COUNT          731 a         1018 c         1101 g          677 t
ORIGIN      
        1 atgaagccga agcgctcgaa actctccggc tggcccagga agtggacatg ttcgccctgc
       61 cggcagacga tcctttcgcc gatgagccag agaccggggc agcggctgta tgaaatacct
      121 ggatgagtac cgcgacgccc ggttggtcca caaggtgatg gacgagctgc gccgcaccat
      181 cacccgcccg tgggtgatca tggagatctg cggcgggcag actcatgcca tcctgcgtta
      241 cgggctggag cagctgttac ccccggagat cgagctggtc cacgggccag gctgcccggt
      301 gtgcgtgact tcgctggagc tgatcgacaa agccctggag attgcctccc ggccaggcat
      361 gatattcgtc tcgtacggag atatgctgcg ggtccccggc agccggcagg atctattttc
      421 gatccgctca gccggcggcg atgtgcgggt ggtgtattcg ccgctggata ccgtgcgtat
      481 cgcccgggag aacccggata aacaggtcgt tttcttcgcc atcggcttcg agaccacggc
      541 tcccgccaat gccatgagcg tgctgcaggc gcagacattg ggtttggaca atttcagcat
      601 cctggtatct caggtgcgag tcccgccggc gatcgaatct attctcagct ctccccacaa
      661 ccgggtgcag ggatttcttc tcgccgggca cgtcagcgcg gtgatgggtt attgggaata
      721 tccacccctg gccgcccgat acaacgtgcc aatgaccgtt accggctttg aaccgctcga
      781 tatcgctcag ggaattttgg aaacggtgcg catgctggaa gatggcacgg tgggtgtcag
      841 gaatgcctac gcccggctcg tcagcgagcg cggcaaccag cctgcgcagg cagtcatcaa
      901 ccaggtattt atggaatgcg accggaactg gcgcgggatc ggtatgatcc cgatgtcggg
      961 atggaggctg cgaccggaat tcgcgagctt caacgccgaa gtgcgcttcg atgttcagga
     1021 catcaactcc caggagtcgc cgctgtgtat ggcgggcctg gtcctgcagg gactgcgcaa
     1081 gcccagcgac tgcccggctt tcggcaggca gtgcacaccc gaaacgccgc tcggtgcaac
     1141 catggtttcc gccgagggcg cctgtgcagc ctattaccgc tacaagatca ctccagaagg
     1201 agcctgagga tgggcatcag cgaaggacct tcctgccctc tcccccttcc ccaccatgac
     1261 cggattatcc tggggcatgg cagcggcggc aagctcaccg acgagctgat cagccgcctg
     1321 ttctatccgt gcttcgataa ccccgccctg cgccagggaa atgactcggc tgctctcagc
     1381 ctggaaaacg gcgcccggat tgccgtgagc acagatgccc acgtcgtctc gcctttattc
     1441 tttccgggcg gagatatcgg ccggctggcg gtctgcggga cggtaaacga cgtggcaatg
     1501 tcaggggcga tcccccggta tttgacggca gggtttatcc tggaagaagg cctggaggtc
     1561 agcctgctgg gtaaagtgat cgaatctatg cgtgacgccg ccgcagaagc aggggtgcag
     1621 attgccgccg gagacaccaa agtggtccag cgcggcaaag ccgatggcct gtatatcacc
     1681 actgcaggga taggtatcct gccagcggga cgccagattg gcggcgcgca agcccggccc
     1741 ggggatgtgg tcctgctctc gggtccactt ggcgatcacg gcatcgcagt cctggcagcc
     1801 cgcggtgagc tgggctttga gacggatatc cagagcgatg ctgccccgct caaccacctg
     1861 attgcggcga tgctcgccgc cagccccaat gtgcatgtgc tgcgggatcc cacccgcggc
     1921 ggtctgggaa ctaccttaaa tgaaattgcc gggcagtcgc aggtcggcat ccagattgat
     1981 gaaaatgcca tcccggtgcg tccggctgtc gcggcagcct gcgaaatgct gggtttcgat
     2041 ccgctgtata tcgccaatga aggtaaactg gtcgccatcg tcgccgcgca ggacgcggag
     2101 cttatcctgg aagtcatgca ccggacacgc tacggcgaag acgcagtgcg gattggtgag
     2161 gtgctcccgg ccccagcagg gcgggtcctg ctgcgctcac gcatcggtgg cgtacgcctg
     2221 gtcgagcgtt tatcgggaga gctcctgccg cggatttgct gagcgcacga ggctgctcaa
     2281 aagcccggct gaattcatgc cgccaggctg cccaggcacg gcaggctgca caatccggcg
     2341 ttattttgaa tttgtcgcat ataattcatc cgtacgaaat ctcaggagga tatcaatatg
     2401 gctaatttag aagatctggt tccggctggc gtcattgtgg gcgaggacgt ccaaaaagta
     2461 tttgcttacg ccaaagccca cgggtttgcc ctgccggctg cgaactgcac cgggacaaac
     2521 acggtgaatg cggtgctgga aacggcgcgt gagatggact ccccgatgat tattcagttc
     2581 tcgaacagcg gggcagcttt ttatgcaggc aagagcctca gcaacgatgg gcagcgggcc
     2641 agtattgccg gcgcaatcgc aggcgccaac cacatccacc agctggccga ggcttatggc
     2701 gtgcgggtta tggtccacac cgatcactgc gccaaaaagc tcctgccctg ggtcgatggc
     2761 ctgctggaag ccggggaaaa gtattaccag gcgcatggta agcctctctt cagctcgcac
     2821 atgctggatt tatccgagct atcgctgcag gaaaacgtgg aaacctgcaa gcgttatctc
     2881 gagcggatga gcaaaatcga catgaccctc gagttcgagc tgggtgtgac tggcggtgaa
     2941 gaagacgggg tggacaatac cggggtcagc agctccaggc tgtacaccca gcctgaagaa
     3001 gtcgcctatg cgtatgaaga gctgatgaaa gtcagcccgc gcttcacgat tgcggcggcg
     3061 tttggcaatg tgcacggcgt atacaaaccg gggaatgtca agctgcgccc ggtcatcctg
     3121 cagaactgcc aggaatatat ccagcagaaa tacaataccc ctgaaaaacc gctcaacctg
     3181 gtctttcacg gcgggtcggg atcgacctcc gaggagatcc gggatgccgt ctcgtacggt
     3241 gtggtcaaga tgaacatcga caccgatctg cagtgggcac tctgggacgg ggtcaaaaac
     3301 tactatgtga agaacgccga ttatctccag gggcagatcg gcaaccccaa aggggcggac
     3361 gaaccgaata agaaattcta cgatccccgc acgtggctgc gggccggtga ggaagctttc
     3421 aaaacgcgct tgcgggcata tttcgaggag ctgaataccg gaaagatgta agctgctgcg
     3481 ccagcagctt gcaagcgctg gcgcagcctt cgaataaggt ataatag
//
LOCUS       DATMXK010000421         2713 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_58973, whole genome shotgun sequence.
ACCESSION   DATMXK010000421 DATMXK010000000
VERSION     DATMXK010000421.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2713)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2713)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2713
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_58973"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(135..953)
                     /locus_tag="VN363_07760"
     CDS             complement(135..953)
                     /locus_tag="VN363_07760"
                     /inference="COORDINATES: protein motif:HMM:NF013460.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b/b6 domain-containing protein"
                     /protein_id="HWQ84445.1"
                     /translation="MSGLKHIPVEERRRLREKKYKKHNLANILTHWFNAAAWLMLLPT
                     GIAIISSPRLGLSPDWLQELFRSMFSSTANLIRFHYTVGLIWVAILTFNILIGFRKYF
                     VPFAAHRMLLDKDDIQWLSIKPLQMLGFMKGKELPPQDAYNAGQKAYMYVVILGTIGI
                     MLSGIVMTFKEIFPPVLKQIAQPVHWVSVASIVAGLMVHVYMGAVFPEEKEAFFSMFS
                     GKVSAWYARAHHEKWYWEKVQEELDWEDEVRKETAAAAIQSPESAPLSESASAD"
     gene            complement(950..1693)
                     /locus_tag="VN363_07765"
     CDS             complement(950..1693)
                     /locus_tag="VN363_07765"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ84446.1"
                     /translation="MPQKKRYGFVIDITRCIDCRACLVACSVENSVSMEHTRIWVHDL
                     GLQGEFPALSRKFMPYNCMHCDNPPCTEVCVSGATYKDPENGLVLVDQEACIGCGFCV
                     DACPYNARYLDEARGVVDKCTACVQRLEVGMQPACVATCVGGSRLFGDFNDPDSEVSQ
                     AIKNARSVQRFDLEYKEGVSTGPNIYYINGDVLQSGLGPHLPRYTASEQVWKKGLVPL
                     MAAAVGGAFLVQATYFTKQLIEGEKEYDE"
     gene            complement(1709..>2713)
                     /locus_tag="VN363_07770"
     CDS             complement(1709..>2713)
                     /locus_tag="VN363_07770"
                     /inference="COORDINATES: protein motif:HMM:NF013717.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWQ84447.1"
                     /translation="GVPWKYPMVPLKIGVFQEIRDAILIEQPYPARGWFISRQNPVLS
                     MPDRKKTLEALGKMEFITVVDVIMNDTAWFADVVLPEASYLERYDPLHVVDGRIFLRQ
                     PVIEPQGEARSALWIYKQLGERLGLADYFQYTDEEDYLRQQLAPLGLDLEMLKTRGYV
                     DPVVEKPAEESTELRFNTPSGKLEVYSETLEKAGFSPWPTWEEPPAPTPDRFYLLTGK
                     VAQHTQMGTQNNELLHKYEDQPRLWLHTSAAETRGIKTNDPVRVTSEVGEIVVQALVT
                     EAIRADCVYMTPGYGHLSKGLRTAFSHGASDSEIHVTYTDPVSGGQALTQTFVTVAKA
                     "
BASE COUNT          574 a          753 c          801 g          585 t
ORIGIN      
        1 gatccagttg aataagttta gccagcatta cacccgagat gtacggaggt ggtcgataaa
       61 ttggtgccaa acagaacgac cccaattctc ttcgaaaagg ggtcgcaatc aagcgaaatg
      121 ggaaactggt gaatctaatc agctgaggca gattcagaca gcggagcgga ttcggggctt
      181 tggatcgcag cggctgcggt ttctttccgg acttcatctt cccagtccag ctcttcctga
      241 accttttccc aataccattt ctcatggtgg gcacgggcat accaggcaga gacttttccg
      301 ctgaacatgg agaaaaaggc ctctttttct tcggggaaga cggcgcccat gtacacatgc
      361 accatcagcc cggcgacaat cgatgccacc gagacccagt ggacgggttg ggcgatctgc
      421 ttgagtaccg gcgggaagat ctctttgaag gtcatcacga tcccgctcag catgatcccg
      481 atagtgccca ggatgaccac gtacatataa gctttctggc cggcattgta agcgtcctgg
      541 ggaggcagct ccttgccttt cataaaaccg agcatttgca gtggcttgat cgacagccat
      601 tggatatcat ctttatccag gagcatgcgg tgggcggcga aaggtacgaa atacttgcgg
      661 aatccaatca ggatgttaaa ggtcaggatg gcgacccaga tcaaaccgac agtgtagtgg
      721 aagcggatca ggttggctgt cgagctaaac atgctccgga acaactcctg cagccagtct
      781 ggcgaaagac ccaggcgcgg ggatgagatg atggcgatac ccgtgggcag cagcatcagc
      841 caggcggcgg cattgaacca gtgagtgagg atattggcca ggttgtgttt cttgtatttc
      901 ttttcgcgca gccggcggcg ttcttctacc gggatatgtt taagcccgct cattcgtcat
      961 actccttctc accctcaatc agctgcttgg taaagtaggt cgcctgcacc aggaaggccc
     1021 cacccacggc agcagccatt aacggcacca gacctttttt ccagacctgt tcagaagcgg
     1081 tgtagcgcgg caggtgtggt ccaagcccgc tttgtaatac atcgccgttg atgtagtaga
     1141 tgtttggtcc ggtggaaaca ccttctttat attccagatc gaagcgctgc acagagcggg
     1201 cgttcttaat tgcctgggat acctcagaat cgggatcatt gaaatcgccg aacaggcgcg
     1261 agcccccgac gcaggtagcc acacaggcgg gctgcatgcc cacctccagg cgctggacac
     1321 aggcggtgca tttatcgacc acgccgcgcg cctcatccaa atagcgagca ttgtaagggc
     1381 aggcatccac acaaaagccg cagccgatgc aggcttcctg atcgaccagg accaggccat
     1441 tttcagggtc tttataggtc gccccgctca cacacacttc ggtgcagggc gggttatcgc
     1501 agtgcatgca gttgtaaggc atgaacttgc ggctcagcgc tggaaactca ccctgcaggc
     1561 ccaggtcgtg gacccagatg cgggtatgtt ccatgctgac actgttttcg acgctgcagg
     1621 cgaccaggca ggcccggcag tcaatgcagc gagttatgtc aatcacaaat ccgtaacgtt
     1681 tcttctgtgg catacaatcc tcccgggctc aggctttcgc cacggtaacg aacgtttgcg
     1741 tgagcgcctg accgccgctg accggatcgg tgtaggtgac gtgaatttcc gagtcgctgg
     1801 cgccgtgact gaatgccgtg cgcaggcctt tggaaagatg cccgtaaccc ggggtcatgt
     1861 acacgcagtc agcccggatc gcttcggtta ccagcgcctg gacgacgatc tcgcccactt
     1921 cgctggtgac gcggacagga tcattggtct tgatgccgcg ggtttccgcc gccgaggtgt
     1981 gcagccacag gcgaggctgg tcctcatact tatgcagcag ctcattattc tgggtgccca
     2041 tctgggtgtg ctgggcgact ttcccggtga gcaggtagaa gcgatcggga gtcggggcgg
     2101 gcggctcttc ccaggtcggc cagggggaga agccggcttt ttccagagtc tctgagtaga
     2161 cttcgagctt gccgctgggg gtgttgaacc ggagctcggt cgattcctcg gccggctttt
     2221 cgaccaccgg atccacgtag ccgcgagttt tgagcatctc caggtccagg ccaagtgggg
     2281 ccagctgctg gcgcaagtaa tcttcttcat ctgtgtactg gaagtaatct gccaggccca
     2341 gccgttcgcc gagctgcttg tagatccaca gcgccgagcg ggcttcgccc tgcggctcga
     2401 tcaccggctg gcgcaggaag atgcgtccat ccaccacgtg gagggggtcg taacgctcca
     2461 ggtaagaagc ttcgggcagg acgacatccg caaaccaggc tgtatcgttc atgatcacgt
     2521 caacgacagt aatgaattcc atcttgccca gggcttcgag ggtcttcttg cggtcgggca
     2581 tggataagac cggattctgg cgggagatga accagccgcg ggccgggtag ggctgttcga
     2641 tcagaattgc atcccggatt tcctggaaga cgccgatctt aagcggcacc atgggatact
     2701 tccagggcac gcc
//
LOCUS       DATMXK010000422         2235 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_15399, whole genome shotgun sequence.
ACCESSION   DATMXK010000422 DATMXK010000000
VERSION     DATMXK010000422.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2235)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2235)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2235
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15399"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2219)
                     /locus_tag="VN363_07775"
     CDS             complement(<1..2219)
                     /locus_tag="VN363_07775"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84448.1"
                     /translation="MNLEVEAENLRLDHSNPKDPALVLENPLQPAYLIFTFPPQTVVE
                     QAFFESSPSTPPPDEANCPYNTNPPAESTPVPPVAARLGRESRLVLRIPPDNAVRIPF
                     TTEGLLNWDGWELSVSALADVPPAPTSQERQAAPDIQAPGRLETAIELPYRLLLSPNS
                     ATSWRHAHQAKTVRSLTELWHTRLMFKDSAGNVSEVTRREPAPLRAIWSPDFNSEKFC
                     ADDLPQFQKPDSDWGVLTPMTPSDRHEIVVLTSAFHGYVRDKDDFRTYEPLPVYAERL
                     MLSPLGGWLTSRGEWDPPAPFKPLIINFPRDNNRWNHYVRDFTRLRPPMGRLNEGLEN
                     VDFNLVGGAGESISGENEGDFDTVLEAAGDARVSTLGMGDLSSILWPGLLGETGDLLN
                     LSEWRHIATQGRDHYVRIVYEGHLYPFGHRAALIKVSERKIRDVKGSDGFNTPLAYLV
                     QRMFIVVRQPLRDYSAPEVASQLENDGRGLPFSNIRLTTLVTPDIARPEDGSQIPGTT
                     YSFWVRLGQGTQAADDFKFSAIAEDIAANFVNFNASLIFVPFGEGHRELVTTAYNASG
                     EERACLVPGQKLTYAPSGGSENTTLDTTRLYFSTHTAPVNKKFGGFLPRLFMATVNLP
                     AVEALLGKPAGTQIALSNKFLGNDPANTSGLFAEIVEEVSSGVLQQARLLSEFAADQA
                     GGFATPHLSISGLTSQFGPLAGDLNNIAQDVFNPSDFFNDVKDAARLFGTLSLADLL"
BASE COUNT          514 a          630 c          631 g          460 t
ORIGIN      
        1 tggagcaggt ctgccaggct cagggtgcca aacagcctgg ccgcatcttt gacatcatta
       61 aagaaatcgc tcgggttgaa cacatcctga gcaatattat tcaggtcgcc agccagaggg
      121 ccgaactggc tggtcagccc ggagatcgag aggtgcgggg tggcaaatcc accggcctga
      181 tcagcggcga actcagacaa cagcctggct tgctgcaaca cacctgacga gacttcttcc
      241 acaatctcgg caaacaagcc gctggtgttt gccggatcat ttccgaggaa cttattggat
      301 aaggcaatct gcgtccctgc aggttttcct aacaaagctt ccacggctgg caggtttacc
      361 gtcgccatga acaacctggg caaaaagccg ccaaactttt tattgaccgg cgcagtgtgg
      421 gtgctaaagt acaggcgggt cgtgtccagg gtggtgttct cgctgccccc agagggtgca
      481 tacgtaagct tctgcccggg aacgaggcag gcccgctcct ccccgctggc attgtaagcc
      541 gtagttacca gctcccgatg cccctcaccg aaggggacaa agatcaaaga ggcattgaag
      601 tttacaaaat tagccgcgat atcctcagca atcgcgctga atttaaaatc atccgctgcc
      661 tgggtaccct gacccaggcg aacccagaaa gaataggtcg ttcctggaat ctggctgccg
      721 tcttccggac gagcgatatc gggcgtgacc agggtcgtca gccggatatt gctgaacggc
      781 aggccgcgcc catcattctc aagctggctg gccacttcgg gcgccgaata gtcgcgcaga
      841 ggctggcgca cgacgataaa catccgctgg acaaggtacg ccaggggtgt attgaagcca
      901 tcgctgcctt tcacatcccg aatcttgcgc tcgctgacct tgatcaaggc ggcgcgatgg
      961 ccgaatggat acaggtgacc ttcatagacg atccggacat agtgatcgcg tccctgggta
     1021 gcgatatgcc gccattccga aagattgagc aaatctcccg tctcgcccag caaaccaggc
     1081 cagagaatgc tgctgagatc gcccattccc agcgtagata cgcgtgcatc tccggcagcc
     1141 tccagcactg tatcgaaatc accttcattt tcccctgaaa ttgattcgcc cgcgccacca
     1201 accagattaa agtctacgtt ttccagacct tcgttcagac gacccattgg cggtcgcaaa
     1261 cgcgtaaaat cgcgcacata gtggttccag cggttattat cgcgtgggaa attgatgatt
     1321 aacggtttaa atggcgccgg agggtcccac tcgccgcgcg aggtcaacca gccgcccagc
     1381 ggagagagca ttaaccgctc ggcatatacc ggcaggggct cataagtgcg aaaatcgtcc
     1441 ttatcgcgca cataaccgtg aaacgcagag gtcagcacaa caatttcatg ccggtcactg
     1501 ggtgtcatgg gggtaagcac cccccagtct gagtccggtt tttggaattg tggcagatca
     1561 tccgcgcaaa atttttccga attgaaatct ggcgaccaga ttgcccgcag aggcgctggc
     1621 tcccggcgag tgacttcgct cacattccca gcagagtcct tgaacattaa gcgggtatgc
     1681 cacagctcgg tcaggctgcg cacagtcttt gcctggtgag catgccgcca ggacgtggcg
     1741 ctgttcggcg aaagcagcaa ccggtaaggc agctcaattg cggtttccag gcggcctggc
     1801 gcctggatat ccggggcggc ctggcgttcc tgggaggtcg gcgccggggg aacatctgct
     1861 aaagccgata cgcttaactc ccacccatcc caattcaaca ggccctcggt agtaaagggt
     1921 atccggacgg cattgtctgg aggaatgcgc aaaaccagcc ggctttcacg cccaagccgc
     1981 gccgcaaccg gtggcacagg tgtggattcc gctggcgggt tggtattata gggacaattg
     2041 gcttcgtcag gaggaggtgt gctgggcgat gattcaaaaa atgcttgctc aacgaccgtc
     2101 tgaggcggga aagtaaagat caaataagcc ggctggagag gattctctaa gacgagcgcc
     2161 ggatctttgg gattactgtg atctaagcgt agattttcag cctctacttc cagattcaag
     2221 aggtcatccg gacgc
//
LOCUS       DATMXK010000423         3822 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_129770, whole genome shotgun sequence.
ACCESSION   DATMXK010000423 DATMXK010000000
VERSION     DATMXK010000423.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3822)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3822)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3822
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129770"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..976
                     /locus_tag="VN363_07780"
     CDS             <1..976
                     /locus_tag="VN363_07780"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWQ84449.1"
                     /translation="LIGLVGTLLPIAAGVGLVYALPESFWGEVINAHKLSFALFIGMN
                     LANSANPVIARILMDLNLLKGELGTMIMTATIVDDMVNWTLFAIILADIAPSAGGSVI
                     PTGGISTTILVVLLFFVLILGIGRKVGPPALAWARRKLPWPHGFIALTAVVILAAGTL
                     SEALGIHAFLGAFLAGVALGSKTEECNEAHEVIEFFAMSFFAPIYFVSIGMTANFITD
                     LDPVLVGLVLAAAMISKLGAVLLGARLAGMQLNRDTWAIAFGLNARGATGIILAGVGL
                     ANGLIDQRIFVAIVLMALITSLISGPAMSTLLRHHQTARQLASQSVVE"
     gene            complement(1133..1780)
                     /locus_tag="VN363_07785"
     CDS             complement(1133..1780)
                     /locus_tag="VN363_07785"
                     /inference="COORDINATES: protein motif:HMM:NF024122.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="haloacid dehalogenase-like hydrolase"
                     /protein_id="HWQ84450.1"
                     /translation="MIVVSDMMGTLTTGSSVLGFVDWIRHNQSRTQARWLMAKITPAY
                     LLAKAGLIDAQRWKLKLMLDSLTWLKEVDPEKFAGVAEWTVEHNLWPRRRADGIAILT
                     RHLQSGAQVTIASSVYEPVALAFASRFGAQAIGTPVQFNSGKVTLAAGLVASEKKIQE
                     VLSRLDVTRVDYAYGDTYMDIPLLESADHPVAVYPDKRLHATARERGWDIFGVPQ"
     gene            1932..2483
                     /locus_tag="VN363_07790"
     CDS             1932..2483
                     /locus_tag="VN363_07790"
                     /inference="COORDINATES: protein motif:HMM:NF024812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84451.1"
                     /translation="MQLPTPHGLISLRQSQPADAEEFRLLRLEALLNHPEAFSADYHF
                     NVQQPVTYWVGRLRSLESQFDLSARGTIYFAEHGDHLIGMCGVQRGDSPKTLHTAWIW
                     GVYVSESWRGLRIADALIDQCAQWAAAHAVTSLKLGVATHNTSAIRCYTRCGFTVYGV
                     EPQAIYNEGKMIDELLMVHYLDQ"
     gene            complement(2491..3660)
                     /locus_tag="VN363_07795"
     CDS             complement(2491..3660)
                     /locus_tag="VN363_07795"
                     /inference="COORDINATES: protein motif:HMM:NF009555.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YgeY family selenium metabolism-linked
                     hydrolase"
                     /protein_id="HWQ84452.1"
                     /translation="MRELLFDGSDATGDLLAFASGLVQIPSPSCQEEAAARFTAEKMA
                     ALGYDEVKIDRFGNVIGRVGHGEKVILFDSHTDTVIVTDAEHWAVPPFSGEIKDGYLW
                     GRGSVDMKSGLAASAYAAALAKNQGLLEGKTVYVTGTVVEEDCDGEGLKHALEENHIQ
                     PDYAIICEPSANLISTGHKGKAQIIIQTRGVSAHGSAPEKGVNAVYEMAEIIQRIEAA
                     NFALQTQGRHGTLVLAQISSTAVSLNAVPSACEVYLDRRLVVAESEQTIQEEMDRITS
                     GKNATWKFDDLQRKTWTGADITYNPLAPAWEISLEHPLAQAFIAACSETFGQPPQESE
                     YWDFSTNAIATVRKGIPTIGFGPGEHKLAHMRDEKCSVDQIVEACAVYTRVINRL"
     gene            complement(3724..>3822)
                     /gene="ssrA"
                     /locus_tag="VN363_07800"
     tmRNA           complement(3724..>3822)
                     /gene="ssrA"
                     /locus_tag="VN363_07800"
                     /product="transfer-messenger RNA"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00023"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: cmsearch."
                     /db_xref="RFAM:RF00023"
BASE COUNT          780 a         1092 c         1108 g          842 t
ORIGIN      
        1 cctgatcggg ctggtaggta ccctgctgcc catcgcggcc ggggtgggtc tggtctacgc
       61 gctgccggag tccttctggg gcgaggtgat caacgcccat aagctgtcat ttgccttatt
      121 tatcggcatg aacctggcga attcggccaa cccggtgatt gcgcgcatcc tgatggacct
      181 gaatctgctc aagggcgagt tgggtacgat gatcatgacc gccacgattg tggacgacat
      241 ggtgaactgg accctgtttg ctattatcct ggcggatata gcccccagcg cgggcggctc
      301 ggtcatcccg accggtggga ttagcaccac gatcctggtg gtgctgctgt tcttcgtgct
      361 gatcctgggg atcgggcgga aagtcggacc gccggcattg gcctgggcgc ggcgcaagct
      421 gccctggccg cacggtttta tcgccctgac ggcggtggtg atcctggcag cggggacgct
      481 ctccgaggcg ttgggcatcc atgcctttct gggagctttc ctggcggggg tagcgttggg
      541 cagtaagacc gaagaatgca acgaagccca cgaagtgatc gagttcttcg caatgagctt
      601 cttcgccccg atctattttg tatcgattgg catgacggcc aactttatca ccgatttaga
      661 ccctgtttta gtcgggctgg tgctcgccgc ggcaatgatc agcaaactgg gggcagtcct
      721 tctgggcgca cggcttgctg gcatgcagct gaatcgagat acctgggcga tcgccttcgg
      781 gctcaatgcc cgcggggcga ccgggatcat cctggccggg gtaggcctgg cgaacgggtt
      841 gatcgaccag cgcatctttg tggcgatcgt gctgatggcg ttgatcactt cattgatttc
      901 aggcccggcg atgagcaccc tgctgcgcca ccaccagact gcccggcagc tggcgagcca
      961 atccgtggtc gagtaaaccc ggaataccgg ggaactccaa ggctgctatt gcgcaaacaa
     1021 ctactgggga gctccaagac ttatataacc caaatcaatc actggggaac tccaaggctg
     1081 ctattgcgca aacaactact ggggaactcc aaggctgcta ttgcgcaaac aactactggg
     1141 gaactccaaa gatatcccag ccgcgttctc tggctgtggc atgcaggcgt ttgtctggat
     1201 agaccgccac agggtggtcg gcggattcga gcagggggat atccatataa gtatcgccgt
     1261 aggcatagtc cacgcgggta acgtccagcc ggcttaaaac ttcctgaatt tttttctcac
     1321 tggcaaccag accggcagcc agcgtaacct tcccggaatt aaattgaacc ggtgttccaa
     1381 tggcctgcgc cccaaaccgg cttgcaaaag caagcgcgac cggctcgtag acgctgctgg
     1441 cgatcgttac ctgggcgccg ctctgaagat gtcgggtcag gatagcgatc ccatccgccc
     1501 ggcgcctggg ccataaatta tgctcgacag tccattctgc aaccccggcg aatttttctg
     1561 gatcgacttc cttcagccag gtgaggctgt cgagcatcag cttcagcttc cagcgctggg
     1621 catcgatcaa gcctgccttt gccaggaggt aagctggcgt gatctttgcc atcagccagc
     1681 gggcctgggt ccggctctgg ttatggcgga tccaatcgac aaagcccagc accgaagagc
     1741 cggtagtaag cgtgcccatc atgtctgaga cgacgatcat aataccctcc tcccagcctg
     1801 catactgaaa attatacccg agcattgctc aacgaccggt tagccggaca gtccggcatg
     1861 atgatcctgc agtagaatcg agctgcacac ctgaacagga tgacaataac ttatccaaat
     1921 ggaaggttcc catgcagctg cccacacccc atggtctaat aagcctccgc cagagccagc
     1981 cagccgatgc tgaggaattc cgcctccttc gcctggaagc gctgctaaac cacccggagg
     2041 cattcagcgc cgattatcac ttcaatgtac aacaacctgt gacttactgg gtaggccgcc
     2101 tgcggtcact ggaaagtcag ttcgatctca gcgccagggg gacaatctac tttgcagaac
     2161 acggcgatca tttgatcggg atgtgcgggg tgcagcgggg agactcaccc aagaccctcc
     2221 ataccgcctg gatctggggg gtgtatgtat cggagtcatg gcgcggattg cgcatagccg
     2281 atgcattgat cgaccagtgc gcacagtggg cggctgccca cgcggtgacc agcctgaagc
     2341 tgggcgtcgc cacccataac accagcgcga ttcgctgcta cacccggtgt ggatttacag
     2401 tctatggggt ggagccgcaa gcgatctata acgaagggaa gatgatcgat gaactgctga
     2461 tggttcacta tctggatcag taatccaggt ttacagccga ttgatcaccc gcgtgtatac
     2521 cgcgcacgcc tcaacaattt gatcaacaga acatttttcg tcccgcatgt gggctaattt
     2581 gtgctcacca gggccaaagc cgatagtggg gatgcccttg cggacggtgg caatggcatt
     2641 ggtgctgaaa tcccagtact cggattcctg gggcggctgc ccaaaggtct cgctgcaggc
     2701 agcgatgaag gcctgcgcca gggggtgctc cagactgatt tcccaggccg gggcaagtgg
     2761 gttgtaggtg atgtctgcgc ctgtccaggt cttgcgctgc aggtcatcga atttccaggt
     2821 agcattcttc ccggaagtga tccggtccat ttcttcctgg atggtctgtt cactttcagc
     2881 cacgaccagg cggcggtcca ggtaaacctc gcacgccgaa ggcactgcat tgagcgaaac
     2941 agcggtgctg gagatctgcg ccagaaccag ggtgccgtgg cggccctgtg tttgcagggc
     3001 aaagttggcg gcctcgatgc gctggatgat ctccgccatc tcataaaccg cgttgactcc
     3061 tttttctggc gcagagccgt gggcagagac gccccgggtc tggataatga tctgggcttt
     3121 gcctttatgc ccggtgctga tcagattggc ggaaggctca cagatgatgg cgtaatcggg
     3181 ttggatatgg ttttcttcca gggcgtgttt gagcccttct ccatcgcagt cttcctcaac
     3241 cacggtgccg gtgacataga ccgtcttacc ttccagcaat ccctggttct tggccagcgc
     3301 ggccgcgtag gctgaggccg ccagcccgga tttcatatcg accgagccgc gcccccataa
     3361 ataaccatct ttgatctcac cactgaaagg ggggaccgcc cagtgctccg catcggtcac
     3421 gatgaccgtg tcggtatgcg aatcgaagag aatcaccttt tcgccgtgac ccaccctgcc
     3481 aataacattt ccaaagcgat cgatcttaac ctcatcgtat cccagggcag ccatcttttc
     3541 ggctgtgaag cgggcagccg cttcttcctg gcaggaaggg ctgggaatct gcaccaggcc
     3601 actggcaaat gccagcaggt ccccggtcgc gtctgaacca tcgaatagta attctctcat
     3661 ttttttcttg ctccgagttt aatgcaaaaa ggcccagaac gtggttcagt gcctttttgt
     3721 ttcaggtgga gatgtcggga atcgaacccg agtccgaaag attcttcccg cgaacgtcta
     3781 cgagcgtagc cggttgagtt ttgtcgccga ggtcacccca aa
//
LOCUS       DATMXK010000424         2562 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_50343, whole genome shotgun sequence.
ACCESSION   DATMXK010000424 DATMXK010000000
VERSION     DATMXK010000424.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2562)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2562)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2562
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50343"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..475
                     /locus_tag="VN363_07805"
     CDS             <1..475
                     /locus_tag="VN363_07805"
                     /inference="COORDINATES: protein motif:HMM:NF013594.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="M48 family metallopeptidase"
                     /protein_id="HWQ84453.1"
                     /translation="GGQIFITNGLLENLSTEDEAAGVLAHEIGHVVGRHSAEHLAKAR
                     LTEGLGGAAVIASYDPENPSSVQTGQIAMVIGQLVNLKFGREDELESDFLGVCFMNEA
                     GYDPQAMLRVMEVLASAGEGQARPPEFFSTHPNPENRIAEIQAAIANLDQCPQSN"
     gene            complement(506..1699)
                     /locus_tag="VN363_07810"
     CDS             complement(506..1699)
                     /locus_tag="VN363_07810"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWQ84454.1"
                     /translation="MKRVAWITALVLGTVAFLLLIWQFRQALIVFLLSLATAAALRPT
                     INRLTARGIKPGLSLGLTYLFLLLMVGGLFVLISRTLLVDLEQITNNITLAYDEIVNT
                     WPESNDQFLSAIASQLPPTTELSGVLTGEAGQQALQGIIGTASGVAGFFSQVFIIVVL
                     SIYWSADRVHFERLWLSLIPVDQRMRAREAWRAVESGVGDYIVGELVQSYLVVLILWV
                     GFTLIGLPQPALVALTSALAWFIPWLGAVLALIPVILIGLTASPQVAILASLLTVAVL
                     ILMEWFVQPRFFSRQRFNSLLLVLAVIMLAQVAGIIGVVLAPVLVAALQISFQHFTAT
                     REVELRADQFSEEFAQLQASLAQLQERLRSQDGDPSPELNSLMERMQTLLDKTTLFLE
                     STNPT"
     gene            complement(1696..>2562)
                     /locus_tag="VN363_07815"
     CDS             complement(1696..>2562)
                     /locus_tag="VN363_07815"
                     /inference="COORDINATES: protein motif:HMM:NF013740.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015562 - efflux transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:1905887 - autoinducer AI-2 transmembrane
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AI-2E family transporter"
                     /protein_id="HWQ84455.1"
                     /translation="TLGVPLPDPAEVNPLDQVSNLIDYSGKAVGGLFAFLALFLLAFY
                     WALEGERTLRGLLLWLPSQSRPGMREIIDEIEGRVGGFVRGQALLCLVIGAAALTAYT
                     LLGLPYALVLGILAGIFEAVPLIGPLLGAVPALLIALTISNEAVIGVVIASVVIQGLE
                     NYLLVPRIMKKTVGVNPIVALLAFIMLSSILGFAGVLLAIPIAAIVQFLLDRFLLNRP
                     PDLGAAIPGRDYASLLRYEYLELTQDIRKVLRKKNEAADDHADEIEDELEKILNRLDN
                     LVDQVNQPEEMA"
BASE COUNT          649 a          700 c          732 g          481 t
ORIGIN      
        1 gggtgggcag atctttatca ccaacggatt gctggaaaac ctcagcacgg aagacgaagc
       61 agccggtgtc ctggcgcatg agatcgggca tgtggtgggc cggcactctg ccgagcatct
      121 cgctaaagcc aggcttacag aaggtctggg tggagcggct gtcattgcgt cttacgatcc
      181 ggaaaatcca tccagcgtcc agactggcca aatcgcgatg gttatcggcc agctggttaa
      241 tcttaaattc gggcgcgaag atgagctcga gtcagatttc ctcggggtat gcttcatgaa
      301 tgaggcaggt tatgatcccc aggcgatgct gcgggtaatg gaagtgctgg cgtcggccgg
      361 tgagggacag gctcgcccgc cggagttttt cagcacccac ccgaaccccg aaaaccggat
      421 cgcagaaatc caggccgcga ttgctaacct ggaccagtgc ccccagtcga actaagaaag
      481 cgggtagtat gggaacagtt caggctcagg ttggattggt cgattccaga aacagggttg
      541 ttttatccag cagggtctgc atgcgctcca tcaggctgtt caattctggt gagggatctc
      601 catcttgcga gcgcaggcgt tcctgaagct gagccagact ggcttgcagc tgggcaaatt
      661 cctcgctgaa ctggtcagca cgcagctcca cttcgcgggt agctgtgaag tgttggaagc
      721 tgatttgcaa agccgcgacc agtacaggcg ccaggacaac accgatgatc ccggcgacct
      781 gggcgagcat gatgactgcc aataccagta aaagggaatt gaagcgctgc ctggaaaaga
      841 agcggggttg gacgaaccat tccattaaga tcaggactgc aacggtgagc aggctggcga
      901 gaatggctac ctgagggctg gcagtcagac caatcaggat gaccggaata agggctaata
      961 cggctcccag ccaggggatg aaccaggcta aagcgctggt caatgccacc agagccggct
     1021 ggggcaggcc aatcaatgtg aaacccaccc acaggatcaa aacaaccaga taggattgga
     1081 ccagctcccc gacgatatag tcgccaacgc ctgattccac tgcccgccag gcttcacggg
     1141 cgcgcatgcg ctggtctacc gggatgaggg ataaccacag gcgctcaaaa tggacccggt
     1201 cggcactcca ataaatagac agaaccacaa taatgaatac ctgactgaag aaccctgcca
     1261 cgcccgaagc tgtaccgata attccctgca gagcctgctg gcctgcctcg ccagtaagca
     1321 cgccgctcag ctcagtcgtt ggggggagct gggaggcgat ggcgcttaag aactggtcat
     1381 tgctttctgg ccaggtattg acaatctcgt cgtaagcgag agtgatattg ttggtgatct
     1441 gttccaggtc gaccaataag gtgcggctga tgaggacaaa cagaccaccc accattaaca
     1501 gcagaaacaa gtaggtcagc cctaatgaga gacctggttt aatcccccgc gcagtcagcc
     1561 ggttgatcgt cggtcgaagg gcggccgccg tagccaggga taacaaaaag acgatcaacg
     1621 cctggcgaaa ctgccagatc aggaggagaa acgccaccgt acccagaaca agggcagtta
     1681 tccaggcgac gcgtttcatg ccatttcctc gggctggttc acctggtcca ccagattgtc
     1741 caggcggttg aggatttttt ccagctcatc ttcaatttca tcggcgtgat cgtcagcagc
     1801 ctcatttttt ttacggagca ccttgcggat atcctgggtc agctcgaggt attcatagcg
     1861 cagcaggctg gcataatcgc gcccaggaat ggcagcgcca agatctggag gtcgattcag
     1921 taaaaaccgg tccaacagga actggactat cgccgcgatt ggaattgcca gcagcacccc
     1981 ggcgaaacca agtatggacg agagcatgat aaaagccagc aaagccacga taggattaac
     2041 gcccaccgtt ttcttcatga tgcggggtac cagcaggtaa ttttccaatc cctggatcac
     2101 aacactggcg atcacaacgc caattacagc ctcatttgaa attgtcagtg cgatgagcag
     2161 agccggcaca gccccaagta agggaccgat aagcggaaca gcttcgaaaa tgcccgccag
     2221 gatgcccaat accagcgcat aaggcaggcc aagcagtgtg taggcagtta atgcggctgc
     2281 tccgatcacc aggcagagca gcgcctggcc gcgcacaaaa cccccgacac gcccttcaat
     2341 ttcgtcgata atctcgcgca taccggggcg cgattgggat gggagccaga gcaataggcc
     2401 gcgcagggtc cgctcgccct ccagtgccca ataaaaggcc aataaaaata acgccaggaa
     2461 ggcgaacaga ccacccactg ccttacccga ataatctatc aaattgctga cctgatcgag
     2521 cgggtttact tctgcgggat ccggcagggg tactcccagg gt
//
LOCUS       DATMXK010000425         3032 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_94734, whole genome shotgun sequence.
ACCESSION   DATMXK010000425 DATMXK010000000
VERSION     DATMXK010000425.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3032)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3032)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3032
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94734"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(61..2646)
                     /locus_tag="VN363_07820"
     CDS             complement(61..2646)
                     /locus_tag="VN363_07820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84456.1"
                     /translation="MLSTVSYSSKVVVKKIARQVPFWLVLISLVGCNMPGFILAQPTE
                     DPMANTPLEGIPTAEPLPPPQQTEVTFRVSVPANSPTDQPVYLVILDEVTGLALNAVS
                     HTMEEESGASPEGKRQYSLSLPFPLGAAVTYRYERGSTAATVGEHITDGRPVRYRIYH
                     VTGPGLVDDVVSRWTDTEFSGATGRIMGQAKDIESGLGIPHLLIAAGGAQTLTASDGS
                     FILEGLPAGVHNLVAYSLEGAYVTFQQGALVAADSTTPAPLEMRRPDTASVIFVVSVP
                     EGTPPAVPVRLAGNLSQLGNTFANLAGGVSTVADRMPVMEPLPDGRYTVSLELPVGAY
                     LSYKYTLGDGFWNAEHAAGGGFSLHHVVVPEGSILVEDTVETWATGGRGAITFDITVP
                     ANTPTSDSVAIQFNPFYGWTEPIPMWKLTETRWVYILYSPLNLVGDLAYRICRDSQCS
                     AADDLATTGATAAGKKVTLSGEAQKIVDSVAGWAQWGAELPQVGPVEGVKARDPFIAG
                     VEFQAGYHPSWQARLPAALDDTRSMGSNWVMFSPTWTYTRNSLPILEPVAGQDATWFD
                     MVAAIEHARLRGLQVALFPQPAFTQDVDSWWQTAPRDFGWWLVWFEQYRLFILHHADL
                     AQRMEVPALVMGGEWLLPAVQIGSLADGSPSGVPADAEQRWRNLIAEVRSRYSGTLLW
                     ASPYGLAPHDSLASVPQFADAVDVLYILFSPPLSLIDTPTAEEIAGEAGRLMDLNVNP
                     VLNRFNKPVILGVEYASADGGGSYCINDGQDGCADPDSLLPTLPDQPGVTLDLQEQAV
                     VYSGVMAALHSRPWISGVFARGYYPAAPLQDKSASVHGKPAQSVLESWFTAWIPVVEP
                     APPGN"
     gene            complement(2673..>3032)
                     /locus_tag="VN363_07825"
     CDS             complement(2673..>3032)
                     /locus_tag="VN363_07825"
                     /inference="COORDINATES: protein motif:HMM:NF042691.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6585 family protein"
                     /protein_id="HWQ84457.1"
                     /translation="VLAPTTGKLLRLPDGLENYPELISQLKACLYPRLLPGLEANLQA
                     GKSLFFGPLAISPRGLVSGSQRWKWEDVETFDVQNGWLEIKTAPKQTLRFPASQIPNV
                     ELLLQLVPQCAPERASA"
BASE COUNT          595 a          930 c          952 g          555 t
ORIGIN      
        1 cccagggtgg gacgatcacg gcggattcag ggggtaaaaa tcaaggcaca accatcacga
       61 tcagtttcct ggcggtgcag gctccacaac cggtatccag gccgtaaacc agctttcaag
      121 cacgctctgg gcaggcttac cgtgaacaga ggctgattta tcctgcaggg gagcggcggg
      181 atagtagcca cgggcaaata caccggaaat ccaggggcgg gaatgcagcg cagccatcac
      241 gccgctgtag acaaccgcct gttcctgtag atccagggtc acgccgggct ggtcgggcag
      301 ggtgggcagc aggctatccg gatcggcgca gccgtcctga ccgtcgttga tgcagtagct
      361 tccaccccca tcagctgaag catactccac gcccaggatg accggcttgt tgaagcgatt
      421 gagcaccggg tttacgttca aatccatcaa tcgtcccgct tcgccagcga tttcctctgc
      481 ggtgggcgta tcgatcaggc tcaggggcgg cgagaacagg atatacagta catccacagc
      541 atccgcaaat tgaggcacgc tggccagcga atcgtgggga gccaggccat aggggctcgc
      601 ccacaagagc gtcccgctgt agcggctgcg cacttcggca atcaggttgc gccagcgctg
      661 ctcggcatcc gcgggcacac ccgatggcga tccatcggcc aggctgccga tctggactgc
      721 cggcaggagc cattcgccgc ccatcaccag ggcaggcact tccatgcgct gggccaggtc
      781 ggcgtggtgc aggataaaca ggcggtactg ctcgaaccat accagccacc acccgaaatc
      841 ccggggtgca gtttgccacc agctgtccac atcctgcgtg aaggcaggct ggggaaagag
      901 ggcgacctgc aggccgcgca ggcgggcgtg ctcgatcgcc gcgaccatat caaaccaggt
      961 tgcatcctgc ccggcgaccg gctcaaggat cggcaggctg ttgcgcgtat aggtccaggt
     1021 gggggagaac atcacccagt tggaacccat gctgcgggta tcgtccaggg cagccggcag
     1081 gcgtgcctgc caggaggggt ggtaaccagc ctggaattct accccggcga taaatggatc
     1141 gcgggctttc acgccttcga ccggaccaac ctgcggcagc tcagcacccc actgtgccca
     1201 gcctgccacg ctgtcgacaa ttttttgcgc ctcgccgctg agggtcacct ttttgccggc
     1261 tgcagtcgcg ccggtcgtcg ccaggtcatc ggcagcgctg cactggctgt cccggcagat
     1321 gcggtaggcc aggtccccca ccagattgag cgggctgtac aggatataca cccagcgcgt
     1381 ctcggtcagc ttccacatcg gtatcggctc ggtccaaccg tagaaggggt tgaactggat
     1441 ggccacactg tcactggtgg gagtgttggc gggaaccgta atatcgaaag ttatcgctcc
     1501 ccgcccgccg gttgcccagg tttcgacagt atcctcaacc aggatgctgc cttcgggaac
     1561 cacgacgtga tgcaagctga atccaccccc ggcggcgtgt tcggcattcc agaagccatc
     1621 ccccagggtg tatttatagc tcaggtacgc gccgaccgga agctccagag aaacggtata
     1681 gcgcccatcc ggcagcggtt ccatgaccgg catgcggtcc gcaacggtgc tgacgccgcc
     1741 cgccagattg gcaaaagtgt tgcccagctg ggaaagattc cctgccagcc gcaccggcac
     1801 agccggagga gttccctccg gtacgctgac cacaaagatc acgctggcag tatccggccg
     1861 gcgcatctcc agcggagccg gagtggtgga atcggccgcc accagagcgc cctgctggaa
     1921 ggtgacatag gcgccttcca gggaatatgc caccaggttg tgcacccctg ccggtaagcc
     1981 ttccaggatg aacgagccat ccgaagccgt cagggtctgc gccccgcccg ctgcgatcag
     2041 caggtggggg atgcccaacc cggattcaat atctttagcc tggcccatta tccggcctgt
     2101 cgcaccgctg aactcggtgt ccgtccagcg gctgaccacg tcatccacca ggccgggtcc
     2161 ggtgacatga tatatgcggt agcgcacagg gcgcccatcg gttatgtgct cgccgactgt
     2221 ggcggcagtt gagccgcgct catagcggta ggtgactgct gcgcccagtg gaaaaggcag
     2281 gctcagactg tactgccgct tgccttctgg actggcgccg gattcttctt ccatggtatg
     2341 gctgacggcg ttcagtgcca gcccggtcac ttcgtccagg atcaccagat atacgggttg
     2401 gtcggtgggt gaattggccg ggacgctgac tcggaaggtg acctccgtct gttggggtgg
     2461 gggcaatggc tcggcagtcg gaatcccctc caggggagta ttcgccatcg ggtcttcggt
     2521 cggctgggcc agaataaatc cgggcatatt gcagcccacc agggagatca gcaccagcca
     2581 gaatgggacc tgacgagcaa tttttttgac gacgaccttt gagctgtagg agacggttga
     2641 gagcatgttc accttcgata cggatcagag aatcaagccg acgcgcgttc cggcgcgcac
     2701 tgcggtacaa gttgcaagag taattccaca ttggggatct ggctggcggg gaagcgcagg
     2761 gtctgtttcg gggcggtttt gatctccagc cacccgttct ggacgtcaaa agtctccacg
     2821 tcttcccatt tccaacgctg gctgccgctc accaggccgc gcggcgagat cgccagcggg
     2881 ccaaagaaca ggcttttccc ggcttgcagg ttggcctcca ggccaggcag caagcgtgga
     2941 tacaggcagg ccttgagctg cgaaatcaac tcgggataat tttctaaacc gtcgggtaag
     3001 cgcagcagct tgccggttgt gggcgccagg ac
//
LOCUS       DATMXK010000426         6161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_107291, whole genome shotgun sequence.
ACCESSION   DATMXK010000426 DATMXK010000000
VERSION     DATMXK010000426.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107291"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1134)
                     /locus_tag="VN363_07830"
     CDS             complement(<1..1134)
                     /locus_tag="VN363_07830"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ84458.1"
                     /translation="MDQNSSPEIPTERASLELLYHTSREFASALDLKVVLQRVLYLSV
                     QYAGAMSGSILVLDDQGVPVDSALIFGKQFRDHSTQQLQVTLESGLAGWVARTRTAAL
                     VLDTSQDERWVQLPDDSPAKTGPKSAVSAPILAHAQLVGVITLVHPEPGTLTNDHLEL
                     VQAIAGQAGVAIHHAQLYQSLQTAHQRYRELFEDSINPIFVTDWQGAIIEANRAALIS
                     SGLVEGTLLGANIFDLHTVSWEAVGDKFTRLVDGRTANYESTVKNALGRNFPVQVQVH
                     RILIDGQPHLQWIMRDITERKDLDRLREDLVSMIYHDLRSPLSNIVSSMDILDTLLDG
                     SPNDGPRALVNIAMRSTERIERLTESLLDISRLEAGQPLGERQW"
     gene            complement(1280..2269)
                     /locus_tag="VN363_07835"
     CDS             complement(1280..2269)
                     /locus_tag="VN363_07835"
                     /inference="COORDINATES: protein motif:HMM:NF024575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase III subunit"
                     /protein_id="HWQ84459.1"
                     /translation="MNWGILGHEWAVQLLHAQLVQGRSKQAYMFTGPPGIGRRTLALS
                     LAQAINCLQPPEPMTPCLTCRSCLQFQNMKHPDLIITQADQVGGVLKVDQIRDIQHRL
                     ALAPYVAQYKVALFLRFEEAHASAANALLKTLEEPPERVIIMLTAPSSESVLPTIASR
                     CEVIRLRPLPYTQVENGLVAGWDIPVEEAHLLGHISGGRPGYALRLSRNEVLLEQRQG
                     WLDDLVHLLFSKRVARFAYAETLTKDKNLPLADVLQTWLAYWRDILLLTTGSGSLLAN
                     IDRQAEMEQLAGQLSIQTARKVIQAAENTLQLLGKNANPRLALEVLMLDLPFC"
     gene            2452..4632
                     /locus_tag="VN363_07840"
     CDS             2452..4632
                     /locus_tag="VN363_07840"
                     /inference="COORDINATES: protein motif:HMM:NF024754.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UvrD-helicase domain-containing protein"
                     /protein_id="HWQ84460.1"
                     /translation="MDLLAGLNTQQQQAVVAGLGPVLILAGPGSGKTRVLTHRIAYLI
                     SGMGVSPYHILAVTFTNKAARVMENRVADLLGDASSGLTLGTFHSICARILRREAEAL
                     PFNANFVIYDTDDQLRLVKQAVIDLDLDPKQHQPARLHAAISNAKNELLLPEQFPVNN
                     YRDEAARKVYERYQNLLLDNNGVDFDDLLLYTAFLLDNKPVIREKYARRYEHVLVDEF
                     QDTNLAQYTLLKHLASFHHNIFVVGDADQSIYRWRGADYRNVIRFEEDFPKTQKILLE
                     QNYRSTQAILDTAMAVIDRNPHRTPKRLFTDRGQGQKAILFEAFDDQGEANYVVDTIS
                     SLVARQKLQPREFAVMYRTNAQSRVLEEAFLKANLPYKLVGAQRFYGRREIKDLIAYL
                     RLVHNPKDQISLERAIQAPKRGIGDKTLFELRAHSQQLKLAPGELLLELGRDPHSVLR
                     EAFKGRSLTSLEQFGILLNRWQVLKTEYNPLDLLDRILDDTDYHAYIDDGSEEGRDRW
                     ENVVELRNLAGERKELSLELFLEGIALVSDQDTVDENANAPTLLTLHAAKGLEFPVVF
                     IVGLNEGTLPHSRSIDDPEEMQEERRLLYVGITRAEERLYLVHVQNRVAYGMNEPALP
                     SRFLEELPEELLADTQPSRNRKQAREQKAAAAFRWPADPQRSSRQELQQRYHPGMRVQ
                     HPLWHEGMVLNSRLQDDDEIVDIFFEDVGLKRVAASLANLQIKP"
     gene            4689..5186
                     /gene="tsaE"
                     /locus_tag="VN363_07845"
     CDS             4689..5186
                     /gene="tsaE"
                     /locus_tag="VN363_07845"
                     /inference="COORDINATES: protein motif:HMM:NF014426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     ATPase subunit type 1 TsaE"
                     /protein_id="HWQ84461.1"
                     /translation="MPILDPHSTEFISRSPEQTRRAGMRLGAMLRPGDVICLVGDLGA
                     GKTTFVQGIAIGWGSLDQVTSPTFVLVNLYRRPDRMRLYHLDAYRLKDAQDATDLDID
                     NLLEGGPLVVEWAERIRPALPGEYLNIQLKYISEQQRDLLFSAHGKHYEDLLAAFRRT
                     VYGVP"
     gene            5186..5854
                     /gene="tsaB"
                     /locus_tag="VN363_07850"
     CDS             5186..5854
                     /gene="tsaB"
                     /locus_tag="VN363_07850"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: protein motif:HMM:TIGR03725.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003725 - double-stranded RNA binding
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     dimerization subunit type 1 TsaB"
                     /protein_id="HWQ84462.1"
                     /translation="MLLALDTSTRTTGLALYDGEQVISETIWTSNDFHTVELAPAIVE
                     AIQKSGKKIDKVEAVAVAIGPGSFTGLRIGLAMAKGIALARHLPIIGIPSLDILAYAQ
                     PLVDLPLAAVLRAGRGRLAIDWYRAGKKGWISQNKLAVTTAEELVEQIEEPTLVAGEL
                     DDDERHILKRKKKLIDLTSPAHSVRRPSFLAELGWRRWQAGQVDDPAALTPIYIHHHD
                     PIPA"
     gene            5851..>6161
                     /locus_tag="VN363_07855"
     CDS             5851..>6161
                     /locus_tag="VN363_07855"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84463.1"
                     /translation="MNEQIKPDVHIRQMELDDLPQVLEIDRSSFSLPWPMSAYRYELL
                     ENPNSLTYVAEIMAAPDVPVVVGVCVVWLILDEAHIATLATDPNYRRMGIARLLLVQA
                     L"
BASE COUNT         1481 a         1662 c         1579 g         1439 t
ORIGIN      
        1 ccattggcgt tcgcctagcg gctggccggc ttcgagacgg ctaatatcca gtaacgattc
       61 agttaatcgt tcaatccttt cggtggaacg catggcaata tttaccagag ctcgtggtcc
      121 atcattcggg ctcccatcca ataaggtatc aagaatatcc atactggata cgatattgga
      181 cagtggggag cgtaaatcat ggtaaatcat ggataccaga tcttcccgca agcgatccag
      241 atccttccgc tcagtaatat cgcgcattat ccattgtaaa tgcggctggc cgtcgatcag
      301 gatgcgatgt acctgtacct gaaccgggaa attccggcca agagcattct ttacagtaga
      361 ttcgtagttc gcggttctac cgtctaccag gcgggtgaat ttatcaccga ctgcttccca
      421 gctgactgtg tgtaagtcga aaatattagc ccccagcagg gttccttcaa ccagcccact
      481 ggaaataagc gcagcccgat tcgcctcaat aatggcaccc tgccaatcgg tgacaaaaat
      541 cgggttaatg ctgtcttcaa acaactcacg gtagcgctgg tgggcagtct gcaaactttg
      601 atacagctgg gcatggtgga tcgcgacccc tgcctgcccg gctatcgcct ggaccaactc
      661 caggtgatcg tttgtcagtg tgcccggttc cgggtggacc agggtgatca caccgaccag
      721 ctgagcatga gccaggatcg gggcgctaac cgcggatttt ggtcctgtct tcgcaggtga
      781 atcatctgga agctgcaccc aacgctcatc ctgactggta tccagaacca gcgctgcagt
      841 gcgggtacgc gccacccagc ctgccaggcc gctctccagg gttacttgaa gctgttgggt
      901 agaatgatcc cgaaattgtt tcccaaagat cagcgctgaa tcaactggaa caccctgatc
      961 atccaatacc agaatgctgc cgctcattgc gccggcatat tgaacggata ggtagagcac
     1021 ccgctgcaag acaaccttca aatccaacgc cgaggcaaat tcccggctgg tatgatagag
     1081 cagttcaagc gatgcccgct cagtgggaat ttccgggctt gagttctgat ccataaacaa
     1141 ccttagtgga ctttaggaac atcctgcttc ctgtagcttt gttcagccag gtttaagatt
     1201 gcttgatttg gcctcaatac agtatagcat attcgtcagg ctcaaataac atccggatac
     1261 ccccagcaat tctcgaaggc taacagaagg gtagatcaag catcaagact tccagggcca
     1321 ggcgtggatt cgcgttcttc cctaatagct gcaatgtatt ttccgcagcc tgaataacct
     1381 ttcgggcagt ctggatggac aattggcccg ccagttgttc catctctgcc tggcggtcaa
     1441 tatttgccag gagtgaacca gaaccagtcg ttaagagcaa aatatcgcgc cagtaagcca
     1501 accaggtttg cagcacatca gccagaggta aatttttatc cttggtaagc gtttctgcat
     1561 acgcaaatcg ggcaacccgt tttgagaaca acaaatgtac caggtcatcc agccaaccct
     1621 ggcgttgttc gagcaggact tcatttctac tcagtcgcaa tgcataacct ggtctcccgc
     1681 ccgaaatatg ccctaataaa tgagcttctt caactggaat atcccagccg gcaaccaatc
     1741 cattttccac ctgtgtatat ggtaaagggc gcaagcggat cacttcacac cgcgaagcaa
     1801 tcgttggcag tacactctca ctgcttggag cggtcagcat gatgatcacc cgttctggcg
     1861 gttcttccag agtcttcagc agcgcgttgg ctgcgctggc atgagcttct tcaaaacgca
     1921 agaacagagc aactttatac tgcgcgacgt acggcgccag agccaggcga tgctgtatgt
     1981 cgcggatctg atctacttta agtactccac ccacctgatc tgcctgggtg attatcagat
     2041 cagggtgctt catgttctgg aattggaggc aggaccggca agtgaggcac ggcgtcatcg
     2101 gctcgggagg ttggaggcaa ttaattgcct gggcgaggct caatgccaga gtccgccgcc
     2161 cgataccggg tggacctgtg aacatataag cttgcttcga acgcccctgc acaagctgcg
     2221 cgtggagaag ctggactgcc cattcatgcc ccagaatacc ccaattcatg gattgatttt
     2281 attccatccc gccctccttg acaacaggat tgcctgattg gcttatttaa ttattcaccg
     2341 cgctggatgc tgtgctatgt tgacatgcta ttctctcgac ggtagaatga actgatttgc
     2401 ttccggtacg tttattgttt aaaggcatct actgatttgt tggagtttgg attggattta
     2461 ctggcaggtc tcaacacaca acaacaacaa gcggtcgtcg ctggattagg accagttttg
     2521 atcctggcag gccctgggtc cggtaaaacc cgcgtgctca cccatcgcat cgcttactta
     2581 atcagtggga tgggcgtcag cccataccac atcctggcgg ttacatttac caataaggct
     2641 gcgcgggtga tggaaaaccg ggtcgccgat ctgctggggg atgcttccag cggcctgaca
     2701 ctgggcacat tccattcaat ctgcgcccgc atcctgcgca gggaagcaga agcactccca
     2761 tttaatgcca attttgtcat ctatgacaca gatgatcagc tgcggttggt aaaacaagcg
     2821 gtcatcgatc tcgatcttga tcccaaacag caccagccag ctcgcttgca tgccgctatc
     2881 tcaaatgcca agaacgaact gctgctccct gaacaatttc cagtgaataa ttaccgggat
     2941 gaagcagccc gcaaagtcta tgagcgttac cagaacctgc tcctggacaa caacggggtt
     3001 gatttcgacg atcttcttct gtatacagct tttctcctcg acaataaacc ggtaatccgc
     3061 gaaaaatatg cccggcgtta tgagcatgtg ttggtggatg aatttcaaga caccaacctg
     3121 gcccagtaca cactgctcaa gcatctggca tccttccatc acaacatttt cgtggtcgga
     3181 gatgccgacc aatcgatcta ccgctggcgt ggagcagatt atcgcaatgt aatccgcttt
     3241 gaggaagatt tccccaaaac ccagaagatc ctgctcgagc agaattaccg ctcgacccag
     3301 gcaattctgg atactgcaat ggcagtcatc gaccgtaatc cacatcgcac tcccaagcgt
     3361 ctgttcaccg accgcggcca ggggcaaaag gcgatccttt tcgaagcttt tgatgaccag
     3421 ggtgaggcca attatgttgt ggatacgatt agcagcctgg tcgcccgcca gaagctccag
     3481 cccagggaat ttgcagtgat gtaccgcacc aacgcccaat ctcgggtctt agaagaggca
     3541 tttctaaagg caaatctgcc atacaaactt gtcggcgctc aacgcttcta tggacggcga
     3601 gagattaaag acctgattgc ttacctgcga ctggtgcaca accctaaaga ccagatcagc
     3661 ctcgaacgcg ccatccaggc cccaaagcgt gggatcggag ataaaacctt atttgagttg
     3721 cgcgcccatt cccaacagct caagcttgct cctggcgagc tgctcctgga attggggcgt
     3781 gacccccact cagtcctgcg tgaggcattc aaagggcgca gtctgacctc cctcgagcaa
     3841 tttggaattt tactcaatcg ttggcaggta cttaagacag aatacaatcc cctcgacctg
     3901 ctggatcgca tcctggacga taccgattac catgcctata ttgacgatgg gagtgaagaa
     3961 gggcgtgacc gttgggaaaa cgtcgtggag ctgcgcaacc tggcaggcga gcgaaaagag
     4021 ctgtcgctgg agctatttct tgaaggaatc gccctggtat ccgatcagga tacagtagac
     4081 gaaaatgcca atgcacctac gttgctcact ttacatgccg cgaaaggtct agaatttccg
     4141 gtagtattta ttgttggtct gaatgaaggt acactgccgc acagccgctc catcgacgac
     4201 cctgaagaaa tgcaggaaga gcgacgtctg ctgtatgtgg ggatcactcg cgccgaagaa
     4261 agactatatt tggttcatgt ccagaatagg gtcgcctatg gaatgaacga accagccctc
     4321 ccttctcggt tccttgagga gctgcccgag gagctgttgg cagacaccca gcccagccgc
     4381 aatcgcaagc aggccaggga gcaaaaagcg gccgcagcct tccgctggcc tgccgaccca
     4441 caacgctctt ctcgccagga gctgcagcag cgttaccatc ccggaatgcg tgttcaacac
     4501 ccgctatggc atgaaggcat ggttttaaac agccgcttgc aggatgacga tgagatcgtg
     4561 gatatattct tcgaagatgt cgggctgaaa cgcgtggcag cctcgttagc aaatttacag
     4621 atcaaaccct gaccctctgg gaaagaacgt ttccaccggg ggcataataa gcgtataatc
     4681 ctaaacaaat gccaattctc gatccgcatt ccaccgaatt catcagccgc agcccggagc
     4741 agacccgccg ggcaggaatg cgtttggggg ctatgctgcg ccccggagat gtaatctgcc
     4801 tggttggcga tctgggagca ggcaaaacca cctttgtcca gggtattgcc attggctggg
     4861 gatcgttaga tcaggtcacc agcccgacct ttgtgctggt taatctttac cgccgcccgg
     4921 ataggatgcg cctctaccac ctggatgcct accgcttgaa agatgcccag gatgcgactg
     4981 atctggacat cgacaatctc cttgagggtg gacctctggt tgtggaatgg gctgaacgga
     5041 ttcgcccggc tttacccggc gaatatttaa atatccagct gaagtatatt agcgaacagc
     5101 aaagggattt attgttttct gcccatggca aacactacga agacctgctg gctgctttcc
     5161 gacgcacggt atatggggtg ccttgatgct attagctctg gatacatcaa cccgaacaac
     5221 aggtctggcg ttatatgatg gtgagcaagt catctcagaa actatctgga ccagcaacga
     5281 ttttcatacc gtcgagctgg cacctgcaat tgttgaagcg atccaaaaat ccggtaaaaa
     5341 gattgataaa gtcgaggctg tagcggttgc gataggtcca ggctcattta ccggtctgcg
     5401 catcgggttg gcgatggcta aggggattgc cctggctcgc catcttccca ttatcggtat
     5461 accatccctg gatatcctgg cttatgccca accgttggtt gatctgccgc tcgcagctgt
     5521 cttacgggct ggacggggcc ggctggcgat agattggtat cgcgccggaa agaaaggctg
     5581 gatctcgcaa aataagctcg cagtgaccac cgccgaagag ctggttgaac agatcgagga
     5641 accaaccctc gtggcaggtg aactggatga tgacgaacgc catattctaa agcgcaaaaa
     5701 gaagctgatc gacctgactt cgcccgccca ttccgttcgc cggcccagtt tcctggcaga
     5761 gcttggctgg aggcgctggc aggccgggca ggtcgatgat cctgcagcat taacgccgat
     5821 ctatatccat catcacgatc caattccggc atgaacgaac aaattaagcc tgatgtgcac
     5881 atccgccaga tggagctgga tgatctccct caggtactgg aaatcgaccg ctcttctttc
     5941 agcctaccct ggccgatgag cgcttaccgg tatgagctac ttgaaaatcc gaattcgttg
     6001 acttatgttg ctgagattat ggctgccccg gatgtgccag tagtcgtcgg ggtttgtgtc
     6061 gtctggttga tcctggacga agctcatatt gccaccctgg ctacagatcc caattatcgg
     6121 cggatgggga tcgccaggct gttactggtg caggctttgc a
//
LOCUS       DATMXK010000427         4345 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_75533, whole genome shotgun sequence.
ACCESSION   DATMXK010000427 DATMXK010000000
VERSION     DATMXK010000427.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4345)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4345)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4345
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75533"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..265
                     /locus_tag="VN363_07860"
     CDS             <1..265
                     /locus_tag="VN363_07860"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SDR family oxidoreductase"
                     /protein_id="HWQ84464.1"
                     /translation="KGAAYSVGKAAQEALILTLAEELKGSGVTANVVRVKTIDIKHER
                     DQQSSPKTAAWTTPEEIISGILYLCSEDARMVNGARLPMYGSQ"
     gene            416..631
                     /locus_tag="VN363_07865"
     CDS             416..631
                     /locus_tag="VN363_07865"
                     /inference="COORDINATES: protein motif:HMM:NF015956.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046914 - transition metal ion binding
                     [Evidence IEA];
                     GO_process: GO:0006811 - monoatomic ion transport
                     [Evidence IEA];
                     GO_process: GO:0055072 - obsolete iron ion homeostasis
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FeoA family protein"
                     /protein_id="HWQ84465.1"
                     /translation="MVKLVSGQSGIVRQLKGGHQFTNQVASMGFTPGAEVTIIQNYNH
                     GPLIVALRDTRLALGRGEAERILVEVL"
     gene            628..2643
                     /gene="feoB"
                     /locus_tag="VN363_07870"
     CDS             628..2643
                     /gene="feoB"
                     /locus_tag="VN363_07870"
                     /inference="COORDINATES: protein motif:HMM:TIGR00437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0015093 - ferrous iron transmembrane
                     transporter activity [Evidence IEA];
                     GO_process: GO:0006826 - iron ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferrous iron transport protein B"
                     /protein_id="HWQ84466.1"
                     /translation="MNTPAASLTIALTGQPNVGKSTVFNVLTGLNQYVSNWPGKTCEQ
                     RIGSVSYNDLEIQVVDLPGTYCLTANSVEEQIARDYIIQGQPDVVAAVVDAASLERNL
                     YLLAELLSLPSPLVLGLNRLDVAEQQGMRVEANVLEAALGIPVIAMVASKGQGVRELL
                     DACVRVAKNPQDYTPNRPEIRADHKAVLNEIETLIAGHVPEPYPLAWVASKLLEGDDE
                     VARMMQAAFGGEQSSDWQAIHRLLMQHEDAFLAVASGRYEWIGRMVRAAVVRPRPGQV
                     TVTDRLDRLATHPTWGLLILLAALGVLFWLTYAIGAPLQAWLELHVVQAGAIWVTTTL
                     NTLPAWLVGLLAQGIIAGVGTVLSLLPILIIFFIGLGVLEDLGYMARAAYVMDRYMHP
                     MGLHGKSFLALFLGFGCNVPAVMGTRIIESPRARLLTILIAPLIPCTARLSVLVLLVP
                     IFFGANAWWVTMLLIALPLGYMLLLGKGLHETALGGEHHAFIMELPLYQMPNLKEIGK
                     GVWQRTMDFLQGAGTIILVVSVLMWGLSHFPTGQIESSYLAALGRGLAPVGQWMGLDW
                     QMMVALLTSFVRKENTIPTLAVLYGAGQDGASLASVLGGILPPAAALAFLAVQVLFIP
                     CVATVAAMLEETRSWRWTLLSVGLLLVISLLTGVLIYQGGRFTGWGI"
     gene            2646..2888
                     /locus_tag="VN363_07875"
     CDS             2646..2888
                     /locus_tag="VN363_07875"
                     /inference="COORDINATES: protein motif:HMM:NF020581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FeoC-like transcriptional regulator"
                     /protein_id="HWQ84467.1"
                     /translation="MLERLLALILEKGAVSPAELATEFDISPEMLESMLSGLERLGYL
                     EAVEGCASGACRGCSTQAGCRPVRMWKPGKRNIKVQ"
     gene            2976..3647
                     /locus_tag="VN363_07880"
     CDS             2976..3647
                     /locus_tag="VN363_07880"
                     /inference="COORDINATES: protein motif:HMM:NF019498.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWQ84468.1"
                     /translation="MPRKEPLAGQRNKLCWEYVEEGRSHPTVQDPVNVGGNLRRLREE
                     RGITIRELAEQSGLAVNTLSLIENGKTSPSVSTLQQLAAALVTPITAFFEAPAAPQSI
                     AFVRKDHRPRAAIELGMLEDLSGGTAVHAVEPFVVTLDPNASSGNREIVHTGYEFVYC
                     LEGRILYTIDHFNYLLEPGDSLLFEAHLPHCWQNMSPDPSRSIMVLYPTDERDQPSVR
                     HFLGE"
     gene            3712..4152
                     /locus_tag="VN363_07885"
     CDS             3712..4152
                     /locus_tag="VN363_07885"
                     /inference="COORDINATES: protein motif:HMM:NF014620.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifB/NifX family molybdenum-iron cluster-binding
                     protein"
                     /protein_id="HWQ84469.1"
                     /translation="MKVAVITDDGSRISQHFGRATFYQVISIENGSIVGRELRNKVGH
                     GQFHGHEHGQDQGHDHGQDHDHGEDAPGVPHGMGQASHDKHLEMAANIDDCEAVICGG
                     MGRGAYQSLLIKGLKPVVTDYSDVDQAALAYAAGTLVDHIDKLH"
BASE COUNT          959 a         1125 c         1327 g          934 t
ORIGIN      
        1 aaaaggcgca gcctattccg tgggcaaagc ggctcaggaa gcactgatcc tgacactggc
       61 cgaagagctg aagggcagcg gggttacggc caatgtggtg cgggtaaaga cgatcgacat
      121 aaagcacgaa cgcgaccagc agtcttcacc caaaactgct gcctggacca cgcctgaaga
      181 gatcatttca ggaatcctgt atttatgctc cgaggatgcg cggatggtta acggggcacg
      241 cttgccgatg tacggcagcc agtagaatcc aggcactgcg ccggcctgga gtaataccag
      301 cactggatat ttggcgttta acaacaggcc ggtcgcagat ccggtagtga caagatctgt
      361 aaaatattac agttaaggat ctcaaataaa tactcaagaa aattcccttt cgcctttggt
      421 taaactggtc agtggtcaat ccgggatcgt acgtcagctg aaaggtggac accagttcac
      481 gaatcaggtc gccagcatgg gattcacacc cggtgcagaa gtgaccataa tccagaatta
      541 caaccacggc ccgttaatcg tggccctgcg ggatacccga ttggccctgg gtagaggcga
      601 agctgaacgt atattggtag aagttttgtg aataccccgg ctgccagcct gaccattgcc
      661 cttaccgggc agccgaatgt ggggaaatcg acggttttca atgtcctgac tggtttaaac
      721 cagtatgtga gtaactggcc tggaaaaacc tgcgaacagc ggatcggttc cgtgagctat
      781 aacgatctcg agatccaggt tgtggattta cccgggactt actgcctgac cgccaactcg
      841 gtagaggagc agatcgcccg cgattatata atccaggggc agccggatgt tgttgcggcg
      901 gttgtggatg ccgcctcgct tgagcgcaac ctgtatctgc tggccgagct gttaagcctg
      961 ccctcgccgc tggtgctggg cctgaatcgc ctggatgtgg ccgagcagca aggaatgcgg
     1021 gtggaggcaa atgtattgga agctgccctg ggcataccgg taattgccat ggtggcaagt
     1081 aagggccagg gggttagaga gctgctggat gcctgcgtgc gggtcgccaa aaatccacag
     1141 gactacactc cgaaccggcc cgaaattcgc gccgatcaca aggctgtgct gaatgaaatt
     1201 gagacgttga tcgcagggca cgtaccggaa ccctacccac tcgcctgggt cgcatccaaa
     1261 ttgttggaag gcgatgatga agtcgcccgg atgatgcagg cagccttcgg cggtgagcag
     1321 agctccgact ggcaggcgat ccaccggctg ctcatgcagc atgaggatgc atttctggcg
     1381 gtggcgagcg ggcggtatga atggatcggg aggatggtgc gcgcggccgt ggttcgcccc
     1441 cggcccgggc aggtcacggt gaccgaccgc ctcgacagac tggccacgca cccaacctgg
     1501 ggattgttga tcctgctggc tgccctgggg gtacttttct ggttgaccta tgcgatcggt
     1561 gcgccgcttc aggcgtggct ggagctgcat gtcgttcagg ccggggcaat ctgggtgaca
     1621 accaccctca ataccttgcc agcctggctg gtgggcctgc tggcgcaggg gatcatcgcc
     1681 ggagtcggca cggtgctgag cctgctgcct atcttaatta tctttttcat cgggttggga
     1741 gtgctggaag atctgggcta catggcgcgg gctgcctatg tgatggaccg ctatatgcac
     1801 cccatgggtc tgcatgggaa aagctttctg gctttatttt taggttttgg ctgcaatgtt
     1861 ccggcagtta tgggaacgcg catcatcgaa tcacccaggg cgcgcttgct tacaatcctg
     1921 atcgcacccc tgatcccgtg tacggcgcgt ttaagcgtgc tggtgttact ggtacccatt
     1981 ttctttggag cgaatgcctg gtgggtgacg atgctcctga ttgccctgcc gctgggttat
     2041 atgctgctgc ttggcaaagg cctgcatgag acagccctgg gcggtgagca ccatgctttc
     2101 atcatggagc tgccgttata tcagatgcca aatctaaaag agattggcaa aggtgtctgg
     2161 cagcgcacaa tggacttttt acagggcgcc ggaacgatta tcctggtcgt ttcggtcttg
     2221 atgtggggac tttcacattt tccgaccggg cagattgaaa gcagttacct ggcagccctt
     2281 gggcgcggcc tggcgccggt tgggcagtgg atggggctgg actggcagat gatggtcgcc
     2341 ttgctgacca gctttgtgcg taaggagaat accatcccaa ccctggcagt cctttacggt
     2401 gccgggcagg atggcgccag cctggccagc gttttgggag gaattctacc gcctgcggca
     2461 gccctggcat tcctggcggt gcaggttttg ttcattccct gtgtggcgac tgtggccgcg
     2521 atgcttgaag aaacccgatc ctggcgctgg actctgctga gtgtgggtct gctgctggtg
     2581 atctcgctgc tcaccggagt cctgatctat cagggcgggc gctttacagg ctgggggatc
     2641 tgagcatgct ggaaaggctg ctggcgctga ttctagagaa aggggcagta tcgcctgccg
     2701 agctggcaac agaatttgat atcagtccgg agatgttaga gagcatgttg agcgggctgg
     2761 agcgcctggg ttacctggaa gctgtcgagg gctgtgcgag cggggcctgc agggggtgct
     2821 ccacccaggc tggctgccgc cctgtccgga tgtggaagcc tggaaaacga aacataaaag
     2881 tacaataaac ccaaaatact ggtcacaaaa ccttgacaat aggcatcaaa caactataat
     2941 aatggtacat ccttcattaa actggaagtg taccaatgcc tcgaaaagaa cccctggcgg
     3001 gccagcggaa taaattgtgc tgggagtacg tggaagaagg tcgatcccat ccaacggtgc
     3061 aggatccggt caatgtgggt ggtaatttgc gccggctgcg cgaggagcgg gggattacga
     3121 tccgtgagct cgccgagcaa agcgggctgg cagtcaacac cttgagcctg attgaaaacg
     3181 gaaaaacctc cccgagtgtc agcaccttac agcagctggc agccgctctg gtgacaccga
     3241 tcacggcttt ctttgaagcg ccggctgccc cacaatccat tgccttcgtg cgaaaggatc
     3301 accggccgcg ggcagcgatc gagctgggca tgctcgaaga tctgagcggc ggaactgctg
     3361 tccatgctgt cgagccattt gtcgtgaccc tggacccaaa tgcaagcagt gggaacaggg
     3421 agattgtcca caccggatac gaatttgttt attgtctgga agggcgcatc ctgtacacga
     3481 tcgatcattt caattatctg ctcgagccag gggacagcct gcttttcgaa gcgcatctac
     3541 cgcactgctg gcagaatatg agccccgatc cttccaggtc gatcatggtg ctgtacccca
     3601 cggacgaacg ggaccaaccc agcgtgcgcc attttctagg cgaatgaggg ttccatacca
     3661 ggaaccgtac acaatctaca tccatgcaac aaaacaacca aggagaaaat aatgaaagta
     3721 gcagtaatta ccgatgacgg cagcagaatc agccagcatt ttggcagagc aaccttttac
     3781 caggtaatat cgattgagaa cggcagcatt gttgggcgcg agctgcgcaa taaggttgga
     3841 catgggcagt ttcacggtca cgagcatggc caagatcagg gtcacgatca cggccaagac
     3901 catgatcacg gcgaggatgc acccggggtg ccgcatggca tggggcaggc gtcgcacgat
     3961 aaacatctcg aaatggctgc gaatatcgac gattgcgaag cagtgatctg tggtgggatg
     4021 gggcgcggcg cttaccagag cctgctgatc aagggcctca agccggtggt gactgattat
     4081 tccgatgtcg accaggctgc gctagcatat gccgcgggca cgctcgttga tcacatcgat
     4141 aagttacact aaattcttag agccagcagg tatgcctctc caggactgtc cttgcgattg
     4201 actggagggg caagctgacc catcctggat gctcacccac gatctttttt attctgaatt
     4261 aatcgcagaa tatctccagc gcccgcggca aatccggcgt aggtggctgg aaacgcgcct
     4321 gcaggctgcc ctggctaagc cgggg
//
LOCUS       DATMXK010000428         8062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_118260, whole genome shotgun sequence.
ACCESSION   DATMXK010000428 DATMXK010000000
VERSION     DATMXK010000428.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118260"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..455)
                     /locus_tag="VN363_07890"
     CDS             complement(<1..455)
                     /locus_tag="VN363_07890"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84470.1"
                     /translation="MASRFQATGSKAIRTMWISEHDSQLIAHLESRGFERDEYHMVYL
                     QQSLAGTILQAQPPLGFRLCNVAGEHEAEQRAQVSYVTFQSSKPFDQYLARYRRFMHS
                     PAYALDRDLVVEAPDGQFAAFCIFWLDAINRTGYFEPVGVHPDFRRKGLG"
     gene            complement(462..752)
                     /locus_tag="VN363_07895"
     CDS             complement(462..752)
                     /locus_tag="VN363_07895"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84471.1"
                     /translation="MLRQFRDDNDLDGMRRILLAGRMDSTSADYIYPGDLNWWLYYLD
                     QDWQDRIYILESDLDDRILGWVLFSPRYNVFDVFMDPDPAYQHQRLELFAWA"
     gene            complement(830..1438)
                     /locus_tag="VN363_07900"
     CDS             complement(830..1438)
                     /locus_tag="VN363_07900"
                     /inference="COORDINATES: protein motif:HMM:NF012696.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="carbonic anhydrase"
                     /protein_id="HWQ84472.1"
                     /translation="MNSQPFLHPADLALDRLLTGNRRFITGQSIHPGQTPDRRMEIAQ
                     GQKPFAMLLGYVDSRVPPEIIFDCGLGDLFVIRTAGQVIDRAVLGSLEFGVVELHIPL
                     LVVLGHARCGAVIATVEALEHNLDPEADIQVLVDAIRPAVAIADEQSGDRIANTVRIN
                     IELIIGRLMCSPVLANAVEKGELKIFGAHYALETGLVEILPR"
     gene            1585..2172
                     /locus_tag="VN363_07905"
     CDS             1585..2172
                     /locus_tag="VN363_07905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84473.1"
                     /translation="MEDKSLSDLVSQILPDEVEQTIGLDKAKPAGDNNILIASLKAAA
                     GQSGSQVENAIDQFLKGEGDLLETTRSAVSRSKTTAKNEVAAFLEEKFKLSPAVAKLI
                     AMLLVKIFPAISELTGETAAKKKKPRRKKDPKPTASSKKKPSKKKAKPRPKTTASKPA
                     AKKKKTSTSSSKKKATTRKKPKKKTRTTTSVDSAA"
     gene            complement(2279..2974)
                     /locus_tag="VN363_07910"
     CDS             complement(2279..2974)
                     /locus_tag="VN363_07910"
                     /inference="COORDINATES: protein motif:HMM:NF012976.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84474.1"
                     /translation="MIRWISVIFMAALLISGCTASATSPVPAQPTRVNLPVIESAVTP
                     TPPKQDPTIIEAPTGQAPTPESTAAEIKCISPAALTPALTEGPYFKSGSPERNSLLDP
                     GIPGTRLTLTGYVLTADCQPVANALLEFWQADANGVYDNAGYTLRGHQFSDENGKYLL
                     ETVIPGIYTGRTEHIHFKVQAPGGPVLTSQLFFPDVAENQSDRIFDPSLVLPLVPVGD
                     GQTAEFNFIIALQ"
     gene            complement(3130..3822)
                     /locus_tag="VN363_07915"
     CDS             complement(3130..3822)
                     /locus_tag="VN363_07915"
                     /inference="COORDINATES: protein motif:HMM:NF019500.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ion channel"
                     /protein_id="HWQ84475.1"
                     /translation="MKQNELPFRDGTTLSRYHPSAWLLIAQLVLLVLYASFDGSHSER
                     VLISIAGTVILVLVVWVIYRSPAVNWIAWVLSVPALVFSLWTIVSDNSTILMLSSLFE
                     SGLYFYAAAGLIAYMMGDYRVTTDELFAAGATYTLLAWGFGYAYLVVEVLVPGSFLGP
                     LNQDRPATFIELLFVSFTNLSATGLSDIVPTSAPARVLIMLEQFAGVGYIAVVISRLV
                     GFMGQRKDIRPH"
     gene            complement(3976..5214)
                     /locus_tag="VN363_07920"
     CDS             complement(3976..5214)
                     /locus_tag="VN363_07920"
                     /inference="COORDINATES: protein motif:HMM:NF012321.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VWA domain-containing protein"
                     /protein_id="HWQ84476.1"
                     /translation="MTQNSLSIQMDTDQTLIAQEESSQRVLEIRIQAPNSAAHGKRLS
                     LNLALVLDRSGSMSGEKLDFVKQAAAHVLDLLQDQDTLALVAYDDEITLLSPSTRVSR
                     ENRIDLQRQISRLRPGGTTFLSGGWLAGCHEVAAAAQNGTLNRTLLLTDGLANVGETD
                     LEILAHQARELFRRGVSTTTFGVGEGFNEHLLEAMSNQGGGNFYYIETPGDIPELFLR
                     EFKELAAVTARDVEISLEFPPQVIPQVLGGWVTEYSGGRLHISLGNLYSGHDQDIYIR
                     LAMPPAGAGGEPVVKAWVLGKADAGQLYEAEAEVNFQYADKDSAEAAPRNRDLLERYA
                     VVELADTATEALKLERQGMNAEANQLLDRSINMNRPYLNPGVAGDYQKLSERMKQGMV
                     ESDRKQTHYSSYNQKRKREQ"
     gene            complement(5225..5836)
                     /locus_tag="VN363_07925"
     CDS             complement(5225..5836)
                     /locus_tag="VN363_07925"
                     /inference="COORDINATES: protein motif:HMM:NF024803.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MerR family transcriptional regulator"
                     /protein_id="HWQ84477.1"
                     /translation="MPNQQYTIRELAERANTTIRTIRYYTEEGLLPPPVIQGKNAYYT
                     RDHLNRLELVRRMKDAYLPLREIRQVMLSISETEVIERLSDQIQPNKRSVKESNMDDS
                     ALSKSSEALEYISRLMSEQAVFRPLARNQPNQNQPSKHTEPLPAASNLRVSGNSESVI
                     GNWQRIQLASGVELHLRTPADPDLTDRVEQLISFAMKLFRRPA"
     gene            complement(5888..6220)
                     /locus_tag="VN363_07930"
     CDS             complement(5888..6220)
                     /locus_tag="VN363_07930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84478.1"
                     /translation="MITAVAEFVQFSNLHCKPVDSQFEALVEAQVKGQVQEILEAWEE
                     KLAFSIDNRLAAIAGSWAIYGLAQDWSHSNKRLAIETFVENITPIINVTLGIEEAAGV
                     SQAEDIPY"
     gene            complement(6320..6502)
                     /locus_tag="VN363_07935"
     CDS             complement(6320..6502)
                     /locus_tag="VN363_07935"
                     /inference="COORDINATES: protein motif:HMM:NF012655.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TetR/AcrR family transcriptional regulator"
                     /protein_id="HWQ84479.1"
                     /translation="MSNKPVVEAKVDPRIKRTRALLVQAFTELLAEKGFQAISVQDIS
                     ERATVNRTTFYLHFPD"
     gene            6551..7072
                     /locus_tag="VN363_07940"
     CDS             6551..7072
                     /locus_tag="VN363_07940"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ84480.1"
                     /translation="MASLTVSNLENQINLYQHTPGFQLHWREGSRTALGTGEQDLLLL
                     IEEPTAQKYRRVTGLYHFAIVFPNRRELARAVARLFSLQVRNYPTDHIQTKTTYLDDP
                     EGNGIELYAESPVDGVMGIRDGSFFAFRADGNPSDGREPLDAVNLNVWLICFAHRRSA
                     ASPILHHPPHSAI"
     gene            complement(7002..7568)
                     /locus_tag="VN363_07945"
     CDS             complement(7002..7568)
                     /locus_tag="VN363_07945"
                     /inference="COORDINATES: protein motif:HMM:NF039496.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5655 domain-containing protein"
                     /protein_id="HWQ84481.1"
                     /translation="MAEMRDWQGMNEMSARLLLERTGEDLKTWNERVERQGFSDEKSL
                     RAWLTEQGVTGYAQSLLVMEQFGYPEFMLSSADELIEQQYADRPGLKPIFDALVAAAA
                     RLGEVTIQARKTYVSLVTPRRTFVRIQPTTQDRIDLGLRIDGQEPGGRLKPSTIQETM
                     RLQIGLKSLNEVDDEVLGWLQIAYEQNK"
     gene            complement(7633..>8062)
                     /locus_tag="VN363_07950"
     CDS             complement(7633..>8062)
                     /locus_tag="VN363_07950"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84482.1"
                     /translation="VTTIYAEPLTQPGFPPFSLEAQLCQLASQLKQTKGENEVWRIVY
                     AWMRQDVVAPWQGAAERIQAGIGKRQLLDVFHEKKLKIFTTTRYELPEATRVLAASLP
                     AEPVQQLLTDCRTNRPEVWNLLVKQIKQGIDQRKEQDDPE"
BASE COUNT         1997 a         2156 c         2050 g         1859 t
ORIGIN      
        1 cctaaacctt tacggcgaaa atccgggtgc acaccaaccg gctcgaagta accggtgcga
       61 tttattgcat ccagccagaa gatacagaaa gcagcgaatt ggccatccgg agcttcaacc
      121 accaggtccc gatccaatgc ataggctggc gagtgcataa aacggcggta gcgggcaagg
      181 tattgatcga aaggcttcga tgactggaat gttacatagg aaacctgagc gcgctgctca
      241 gcttcatgtt caccggcgac gttgcacagg cggaaaccca ggggaggctg agcctgcaaa
      301 atcgtaccag cgaggctctg ttgaaggtag accatatggt attcatcccg ctcaaaaccc
      361 cggctctcca gatgagcaat cagctgggaa tcatgctcag aaatccacat cgtacggatg
      421 gccttactgc cggtcgcctg aaaccggcta gccatctgtg cctacgccca ggcgaacaat
      481 tccaggcgtt ggtgctggta agcaggatct gggtccataa acacatcaaa gacattatag
      541 cggggtgaaa acagcaccca gcccaggatg cggtcatcca ggtcactttc taaaatatag
      601 atcctgtcct gccaatcctg gtccaggtaa tacagccacc agttcaggtc gcccgggtaa
      661 atataatcgg ctgaagtaga atccatcctg cccgcgagca ggatgcgccg catgccatcc
      721 agatcattgt catcccgaaa ttgacgaagc atagataatc tcgccagccg tacccaatct
      781 aatctgctgt tgacgggatt cgggtacctg atctcggcta tagcttgtat tacctgggca
      841 ggatttcaac cagcccagtc tccagggcat aatgcgcgcc gaaaattttc aattcccctt
      901 tttcgactgc attggcaagg accggcgagc acatcaggcg cccgatgatc aattcaatat
      961 ttatccggac tgtattcgct atccggtcac ctgactgctc atcagcaatc gcaacggccg
     1021 gacggattgc atccaccagg acctggatgt ccgcttccgg atcaaggttg tgctccagag
     1081 cttcgactgt ggctataacc gccccgcatc tggcgtgacc cagcacaacc agcaagggga
     1141 tgtgcaattc tacaaccccg aattccagac tgcctaaaac agcccgatcg atgacctgac
     1201 ccgcagtccg gattacgaat agatcgccca gaccgcagtc aaagatgatt tccggcggca
     1261 cccgcgaatc cacataaccc agaagcatgg caaatggctt ttggccctgg gcgatttcca
     1321 tacggcgatc aggcgtctgc ccaggatgga tggattggcc tgtaataaat cgccggttac
     1381 ctgtgagcag ccggtcaagc gccagatcgg ctggatgcaa gaatggttgg ctgttcatgg
     1441 atacttctcc tgaccgtaga taatagcctt gataggattg taaaaaggga aaataacccc
     1501 tgaatcaagg atgatccagg cggataaatt gtgcgcgtac cagtatactt gaataacata
     1561 caagactccc caggaggaaa atcgatggaa gacaaatcac tttcggatct cgtatcacaa
     1621 atcctcccag acgaggttga acagacgatt ggattggata aagctaaacc ggccggtgac
     1681 aataacatcc ttattgccag cctgaaagcg gctgccgggc agtcgggcag ccaggtcgag
     1741 aacgccatcg accagttcct taagggagaa ggagatttgc tggagacgac ccgcagcgca
     1801 gtctcccgca gcaagaccac ggctaagaat gaggttgctg ctttcttaga ggagaaattc
     1861 aagctttccc cggcggttgc caaactgatt gccatgctgc tggtgaagat cttcccggcc
     1921 attagtgagc ttaccggtga gactgctgcg aagaaaaaga aacctcgccg taagaaagat
     1981 ccaaagccca ctgcgagcag caagaaaaag ccgagcaaaa agaaagccaa accccgtccc
     2041 aaaaccacgg cttcaaagcc agctgctaaa aagaaaaaaa cctctacctc aagcagtaag
     2101 aagaaggcaa caaccaggaa gaaacctaag aagaaaacca ggacaacaac gagcgttgat
     2161 tctgcagctt gaaattgacc gccggtccat taaataattc aaaagcgctt tccatggatg
     2221 gagagcgctt ttgttattcc ttcctgagga ggaaaattct ccggttcgtg cagacagctt
     2281 actggagggc gatgataaag ttaaattcag ctgtctgccc atcaccgacc gggacgagtg
     2341 gcagcaccag acttggatca aagatgcgat cagattggtt ttcagcgaca tccgggaaga
     2401 acagctggct ggtgaggacc ggaccgccag gcgcctgaac tttgaaatgg atatgttccg
     2461 tgcgtccggt atagatgccg gggatcaccg tttccagaag gtactttcca ttttcgtcgc
     2521 tgaactggtg accgcgcagg gtatacccgg cattgtcata gaccccgttc gcatctgcct
     2581 gccagaattc cagcagggcg ttggcaaccg gctggcagtc agcagtcagt acatacccgg
     2641 tcagggtgag cctggtccca ggaatgcctg ggtccagcag cgagttgcgc tcaggtgagc
     2701 ccgatttaaa atacggacct tcggtcaggg ctggagtcag ggccgccggg ctgatgcact
     2761 tgatttccgc cgctgtggat tccggtgtag gagcctgtcc tgttggagct tcgattatgg
     2821 tcggatcttg cttcgggggt gttggggtga ctgctgattc gatgacaggc aaattcaccc
     2881 gggtaggctg agctggaaca ggcgaggtag cagaggcagt gcagccagat atcaataggg
     2941 ctgccatgaa aataacacta atccagcgga tcataattga tcaatccttt tgtaacagga
     3001 catctgagct gttttaacag ccagaataaa tacgatgtat ctggtcggat tatagcaggg
     3061 ggatccgcag agaatgtgag aaaatattta gtaagccgtg gtaatcacca attccgatat
     3121 ttagaattct tagtgcggcc ggatatcttt gcgttggccc ataaacccca ccaaccggga
     3181 tatcaccaca gcaatatagc ctaccccggc aaactgctcg agcataatga gcacacgggc
     3241 tggcgcgctg gtaggcacga tatcacttaa tccggtcgcg gatagattgg taaagctgac
     3301 aaatagcagc tcaataaacg tcgccgggcg atcttgattc aacggtccaa gaaaactacc
     3361 cgggactaaa acctcaacca cgagatatgc atatccaaat ccccacgcca gcaaagtata
     3421 ggtagcccca gcagcaaaca actcatccgt cgttacgcga taatccccca tcatataggc
     3481 gatcaggcca gcggcggcgt agaagtacaa accggattca aataatgagg aaagcatcaa
     3541 gatcgttgaa ttatctgaga cgatcgtcca gagcgagaag actaacgccg ggactgaaag
     3601 cacccaggca atccagttta cggcgggact gcgatagata acccagacga ccaggaccag
     3661 gatgaccgtg cctgcgatgc tgatcaggac acgctcagaa tgactgccat caaagctggc
     3721 atacagcacc agcaggacca gctgagcgat cagtaaccag gcagacgggt ggtagcggga
     3781 aagggttgta ccgtcgcgaa acggcaattc attctgtttc atgctggtaa gcggctggta
     3841 taagcgatgg atggtaaatc agacatgccc tttgttccca tctttaaaga gataaacaag
     3901 gcgaataaag ctgagtaagt ggacctttgt ctattttacc cttgcgatca acattcaatg
     3961 agaggaatgg gaaagttatt gttccctttt acgtttctga ttgtagctgc tatagtgcgt
     4021 ctgtttgcga tccgattcaa ccatcccctg cttcatccgt tcagataact tctgataatc
     4081 tccggcaacg cccggattaa ggtaaggccg gttcatgttt atggatcgat caagcagctg
     4141 gttagcttca gcattcatcc cctggcgctc aagtttcagc gcctcagtgg ccgtatctgc
     4201 cagttcaacc accgcgtatc gttccaggag atcacggttc ctgggagctg cttctgcgct
     4261 atccttgtcg gcatactgga aattaacttc agcttcagct tcgtataact gacctgcatc
     4321 tgctttaccc aatacccatg ccttcacaac aggctcaccg ccggcaccgg ctggtggcat
     4381 cgcaagccgg atataaatat cttgatcatg gcccgaataa agattaccca gggagatatg
     4441 cagacgccct ccagaatatt cagttaccca accgccaagg acttgcggga ttacctgggg
     4501 aggaaattcc aggctgattt ccacgtcgcg tgccgtaact gcggcaagtt ccttaaactc
     4561 ccgtagaaaa agttcgggaa tatcccctgg cgtttcgata tagtaaaagt tgccaccacc
     4621 ctggttggac attgcttcca gcaaatgttc gttaaagcct tcaccaaccc cgaaggtcgt
     4681 ggtagataca ccccgcctga acaactctct ggcctggtga gctaaaattt ccagatctgt
     4741 ctccccaaca tttgccagtc catcggtaag cagcaaagtc cggtttaagg tcccgttctg
     4801 tgcagcagca gctacctcat ggcagccagc caaccaacca ccactcaaaa aagtagttcc
     4861 gccaggtctg agtcttgaga tctgacgttg gaggtcaatc cgattttccc tggaaaccct
     4921 ggtactcggt gacaggagcg tgatttcatc atcatacgcg accagggcaa gcgtatcctg
     4981 atcctggagc agatcgagca cgtgagcagc ggcctgcttg acaaagtcca gcttctcacc
     5041 actcatcgat ccactccggt cgagaaccaa ggccagatta agcgacaggc gtttgccatg
     5101 ggcggctgag tttggcgcct ggatgcggat ttcgaggacc cgctgggatg attcttcctg
     5161 ggcgattaag gtctggtctg tatccatctg aatagatagt gaattctgag tcattttgcc
     5221 ctcctcaggc aggtcgccgg aatagtttca tagcaaacga gattaattgc tccacacgat
     5281 ccgtcaaatc cggatcagct ggcgtgcgca gatgcagctc aaccccagaa gccagctgaa
     5341 ttctctgcca gtttccaatt acagattcag aattccctga tacacgtaaa ttgctggcag
     5401 ctgggagagg ttcagtgtgc tttgatggtt ggttttggtt aggttgattt cttgccaggg
     5461 gacgaaaaac tgcctgttca ctcattaatc gggatatata ttccagggct tcagaactct
     5521 tgctcaaggc agaatcgtcc atatttgatt cttttaccga tcgtttattg ggctgaatct
     5581 gatcggataa tctttcgatg acctctgttt cgctaatcga caacatgacc tgccggattt
     5641 cacgcagggg tagataggca tctttcatcc tgcgaaccag ctccagccgg ttaagatggt
     5701 cacgagtgta atatgcattc ttcccctgga taacgggcgg aggtaaaagg ccttcctctg
     5761 tataataacg gatcgtgcgg attgtagtat tggccctctc ggccagctcc ctgatcgtgt
     5821 attgttggtt tggcatagtc atcactcccg gtttctatct agatgttacc agtattacgt
     5881 aatgatgtca atatggaata tcttcagcct gagatactcc tgcagcctct tcgatgccca
     5941 gggttacgtt gataatgggg gtgatatttt caacgaatgt ctctatggct aaccgtttat
     6001 ttgagtggct ccagtcctga gccaacccgt agattgccca gcttccggcg atcgcagcca
     6061 gccggttgtc gatcgagaag gccagctttt cttcccacgc ctccaggatt tcctgcacct
     6121 gccccttaac ctgggcctcc accagggctt cgaactgtga gtcgaccggt ttgcagtgca
     6181 aatttgaaaa ctggacaaac tctgccacgg cagtaatcaa agagcgcaga ttttctgggg
     6241 agtaatggca gatattcaga gagcgctttt cgatctcctg gtggaataac tggctggcag
     6301 aatactcgag cagggcgaat taatctggaa aatgtaaata aaacgttgtg cgattgaccg
     6361 ttgctctctc tgagatatcc tgcaccgaga tggcctggaa tcctttttcg gccagtaatt
     6421 ccgtaaatgc ctggacaagc aaggcccgcg tacgttttat tcgaggatcg actttcgctt
     6481 ctaccaccgg tttattcgac atatctcctc cagtgtcgat tattccatcc accccgacac
     6541 caatcttggg ttggcttcgt taacagtctc gaacctggag aatcagatca atttatacca
     6601 gcacacgcct ggctttcagc tccactggcg cgaaggcagc cggactgccc tgggcaccgg
     6661 cgaacaggac ctgctgctgc tcatcgagga gccaactgct caaaagtacc ggcgggtgac
     6721 tggcctgtac cattttgcaa tcgtcttccc caaccgccgc gagctggctc gcgccgtagc
     6781 gcgcctgttc agcctgcagg tgcgcaacta tcccaccgat cacatccaga caaaaaccac
     6841 ctatctggat gatccggagg gaaatgggat cgagctgtac gccgaatccc ctgtagatgg
     6901 ggtcatgggg attcgggatg ggagcttttt tgctttccgc gccgatggca atccgagcga
     6961 tgggcgcgag ccgctggacg ctgtgaactt gaatgtctgg ctcatttgtt ttgctcatag
     7021 gcgatctgca gccagcccaa tacttcatca tccacctcat tcagcgattt aaggccaatc
     7081 tgcagacgca tcgtctcttg aattgtggat ggcttaagcc gcccacccgg ttcctgtcca
     7141 tcgattcgca aaccaagatc aatacgatct tgagttgtag gctggatgcg cacaaaagtc
     7201 cggcgaggcg tgaccagcga tacataggtc ttgcgtgcct gaatggtcac ctcccccagc
     7261 cgtgcagctg ccgcgaccag cgcgtcgaag atcggtttta gccccgggcg atcggcgtac
     7321 tgctgttcga tgagctcgtc ggctgaagag agcataaatt ccggatagcc gaattgttcc
     7381 atgaccagca aggactgggc atacccggtc acaccttgct ccgtcagcca ggcgcgcagg
     7441 cttttttcgt cgctaaaacc ctgtctttcg acccgttcat tccaggtctt caagtcttca
     7501 ccggtccgct caaggagcaa gcgagccgac atttcgttca tcccttgcca atcccgcatc
     7561 tcagccatcc aagactctcc ttgattttgc tgtcactcta aacaccttac cgactccgcc
     7621 tggctgcagg ggctactcgg gatcatcctg ttctttgcgc tggtcgattc cctgcttaat
     7681 ctgttttacc agcagattcc atacctcagg gcgattcgta cgacaatctg tgagcagctg
     7741 ctgcaccggt tctgccggga gcgaggcggc cagaacacgg gtcgcttcag gcagctcgta
     7801 gcgtgttgtt gtgaaaatct tgagcttctt ctcgtgaaaa acatccagga gttggcgctt
     7861 gccgattcca gcctggatcc tttcggctgc cccctgccag ggagcaacga catcctggcg
     7921 catccaggca tatacaatcc tccagacctc gttctcacct ttcgtttgct tgagctgact
     7981 ggccagctgg caaagctgag cttccaggct gaatggaggg aaaccaggct gggttagcgg
     8041 ctcagcgtaa atcgttgtga cc
//
LOCUS       DATMXK010000429         2970 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_119708, whole genome shotgun sequence.
ACCESSION   DATMXK010000429 DATMXK010000000
VERSION     DATMXK010000429.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2970)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2970)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2970
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119708"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            219..289
                     /locus_tag="VN363_07955"
     tRNA            219..289
                     /locus_tag="VN363_07955"
                     /product="tRNA-Gln"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:251..253,aa:Gln,seq:ctg)
     gene            complement(344..1714)
                     /locus_tag="VN363_07960"
     CDS             complement(344..1714)
                     /locus_tag="VN363_07960"
                     /EC_number="3.2.1.21"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559538.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008422 - beta-glucosidase activity
                     [Evidence IEA];
                     GO_process: GO:0030245 - cellulose catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GH1 family beta-glucosidase"
                     /protein_id="HWQ84483.1"
                     /translation="MKRLDFPVDFIWGAATSAYQVEGAWNEAGKGESIWDRYSHTPGK
                     VLENENGDVACDHYHRWQADIQLMKEMGLPAYRFSTAWTRIYPAGRGQINQPGLDFYS
                     RLVDGLLEAGIEPVICLYHWDLPQSLQDQGGWPARETAYAYGEYAHTVTRFLGDRVKR
                     WITHNEPTCAGLLGYQSGAHAPGIKDWKLALPAIHHLLLSHGLGVQAVRANVPGGQVG
                     MVIDPLPCEPATDSPEDYAAARWVDGTHNRWYLDPLYGRGYPAEILAEHIRLGNLPQS
                     GPGYVQTGDYELIALPTDFLGLNYYRRGVVRAGEVENVGSPVATSDPDPRHTEMGWEI
                     HPWGLFNLIMLMHLTYRPKALFVTENGASYSDGPDSSGRVHDERRIHYLQGHIAAVQQ
                     AVQAGAPVKGYFAWSLMDNFEWAMGYAQRFGLIHVDFKNQKRTPKDSALWFREVIQAN
                     SVEVEV"
     gene            1877..2704
                     /locus_tag="VN363_07965"
     CDS             1877..2704
                     /locus_tag="VN363_07965"
                     /inference="COORDINATES: protein motif:HMM:NF012804.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="class II aldolase/adducin family protein"
                     /protein_id="HWQ84484.1"
                     /translation="MIQTVNRFELDDWIASLAEAGRRMSEIGASEGAAGNLSVCLNAE
                     VELTPRFPLRKDYHLPVAAPGLAGMTVLVSGSGRRLRDLKPSPYENLGAVLIGEDGVN
                     GWLYTAASCRFARPTSEFNTHLAVHNDQMQRSPTASLALIHAQPVYLTYLSHLEDYQD
                     ADTLNRQVLRWQPEAIINFPQGIGVLPFQVPGSYELMEASVNSLRSFELIVWSKHGVL
                     ARSECGFDHAMDNIEYAEAAAHYEYLNLVLENQAAGLSPAELEKIAKIFGTNIPPGY"
     gene            complement(2722..>2970)
                     /locus_tag="VN363_07970"
     CDS             complement(2722..>2970)
                     /locus_tag="VN363_07970"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84485.1"
                     /translation="IVAHIKSEVRPELLDIVLALGRRSPRETAYFVQELLQGPGSMSG
                     GWLARQLLPHLPEDVAVSLRHTLHAVSSQPTRPIPRRE"
BASE COUNT          714 a          790 c          799 g          667 t
ORIGIN      
        1 gcggtgcgga tgaaatccgt agcggtgctg gtttcattgg gagtattgct ttcagtgaag
       61 tttaaatctg tcatttggaa accttaaaat cgaagttgag taagcttaat aagttaccat
      121 tataacaaac agagtgcaac caaagcaaaa aacacaaact gggagcataa tttcgtttga
      181 cgaactgtta cccaggtggt acaatttgtc cgctccattg ggggttcgtc tagtggtagg
      241 acagcagact ctggatctgt atgccgaggt tcgaatcctc gacccccagc ctgccacacc
      301 ccggtatact ctaccggggt gtttttattc agaaccaaca aaactaaacc tcaacttcca
      361 cagagtttgc ctgaatgacc tcacggaacc acaaagcgct gtctttgggc gtacgttttt
      421 ggtttttgaa atcaacatgg atgagaccga atcgctgggc ataacccatc gcccattcga
      481 aattatccat caacgaccag gcgaaataac ctttcaccgg cgcgccagcc tggacagcct
      541 gctggacagc cgcgatatgg ccttgcagat aatgaatgcg ccgttcatcg tgaacacggc
      601 cagaggaatc tggtccatcc gagtagctgg caccattttc ggtgacaaac aatgctttcg
      661 gtcgataggt gagatgcatc agcatgatca ggttgaataa tccccacgga tggatttccc
      721 agcccatctc ggtatgacgc gggtcagggt ctgaagtcgc aaccggactg cctacattct
      781 ccacctcgcc agcccgcacg accccgcgcc ggtaataatt caagcctaag aaatctgtgg
      841 gcaaggcaat cagctcgtaa tcaccagtct gaacatagcc aggtcctgat tggggtaaat
      901 ttcccaggcg gatatgctcc gctaaaattt ctgccgggta gccgcgccca tacaggggat
      961 cgagatacca gcgattatgg gtgccatcca cccaccgcgc cgcggcgtaa tcttctgggc
     1021 tatctgtcgc tggctcacac gggagcgggt cgatcaccat acccacctgc ccgccgggaa
     1081 catttgcacg cactgcctgc acacccagac catgcgacag cagcagatgg tggatggctg
     1141 gcaaggctaa tttccagtct ttgatccctg gcgcgtgcgc tcctgactgg taaccaagca
     1201 gacctgcaca ggtcggctcg ttgtgggtga tccagcgttt gacccggtcg ccaagaaaac
     1261 gcgtgacagt gtgcgcatat tccccatacg cgtaagcggt ctcgcgggcg ggccacccac
     1321 cctgatcttg cagtgactgg ggcaagtccc agtgatagag acagatcacc ggctcaatcc
     1381 cggcttccaa caatccatcc accaggcggc tgtaaaaatc caggcctggc tggttgatct
     1441 ggccgcgtcc ggccggatag atacgggtcc aggctgtcga aaaacggtat gccggaagtc
     1501 ccatttcttt cattaattgg atatcggcct gccagcggtg ataatgatca caggcaacgt
     1561 ccccattttc attctccaga accttaccgg gagtgtgcga ataacgatcc cagatcgatt
     1621 cgcctttgcc agcctcgttc caggcgcctt ccacttgata agccgaagtt gctgcgcccc
     1681 agataaaatc aacgggaaaa tctaagcgtt tcattttgcc atcctatggg ttgggatagg
     1741 tgaattatga atggggagat gaaggatgtc aaggagaagc aagatcctgg aaaatgaagt
     1801 ggaggacctg cttgacatgc ctcgggatcg ctgtatactg aaaagcgtaa aaaattcagc
     1861 taaggaaatg caaagtatga tccaaacagt gaatcgattt gagctggatg attggattgc
     1921 cagcctggct gaagccggca ggcgcatgtc cgagattggc gccagtgagg gtgcagccgg
     1981 caacctatcg gtgtgcctga atgccgaggt ggaattaacg cctcgctttc cactgcgaaa
     2041 agattatcac ctgccggtag ccgcacccgg gctggctggc atgaccgttc tggttagcgg
     2101 gtctggcagg cgattgcgcg atttgaagcc atcaccttat gaaaacctgg gagcggtttt
     2161 gatcggcgaa gacggtgtga atggatggct ttatacggct gccagttgcc gcttcgcccg
     2221 tcctaccagt gagtttaaca ctcatctggc agtccacaat gaccagatgc agcgttcgcc
     2281 tactgccagc ctggcgttga tccacgctca gcctgtttac ttaacctatt tgagtcatct
     2341 ggaagattat caagatgcag acacgctcaa ccgccaggtc ctgcgctggc agccagaagc
     2401 gattataaat tttcctcaag gcatcggggt cttacccttt caggtgcccg ggtcgtatga
     2461 attaatggaa gccagtgtga acagtctgcg cagcttcgag ttaatcgtct ggagtaaaca
     2521 cggtgtactg gcccggtctg aatgtggatt cgatcatgcg atggacaaca tcgaatatgc
     2581 agaagcggct gcccattatg aatatctcaa cctggtcctg gaaaaccagg ctgccggcct
     2641 gtcgccggcc gagctggaaa agatcgccaa aatctttggg acgaacatcc ctccaggcta
     2701 ttgattgtgt cagaagcttg cttattcgcg ccgtgggatc gggcgggtcg gctggctgga
     2761 aacggcatgc aaggtgtgcc ggagcgaaac tgccacgtct tcgggtagat ggggcagtaa
     2821 ttgacgcgcc agccatcccc cagacatcga acctggaccc tgtaataatt cctggacgaa
     2881 gtaagcggtt tcgcgaggcg agcgccggcc cagggccagc acgatgtcca gcaattcagg
     2941 tctgacctcc gatttgatgt gcgccacaat
//
LOCUS       DATMXK010000430         3460 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_129882, whole genome shotgun sequence.
ACCESSION   DATMXK010000430 DATMXK010000000
VERSION     DATMXK010000430.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3460)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3460)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3460
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129882"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..454
                     /locus_tag="VN363_07975"
     CDS             <1..454
                     /locus_tag="VN363_07975"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84486.1"
                     /translation="VLTLLVKNLPQIQVSYYRPIELAEVERPVELPIEIMNIGRTSIN
                     ISQAEVTADQVEIENGSAFIGSLDGGTSAFLDAVGIPQTSGDLELRLTVNYLDDFNQP
                     HVITETLTLSVEAPPELPSGANGMPPEQEGGFAQGLLYFIRGLFGLGS"
     gene            455..1750
                     /locus_tag="VN363_07980"
     CDS             455..1750
                     /locus_tag="VN363_07980"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84487.1"
                     /translation="MMDWRSLLHIVLRNLRRMKLRAAMTAMGVVIGTIAIILLVSIGA
                     GLQRFAFQGVAGSTELTLIEVSQGNNGNAAVASDRPPALLDRRALAEFRNIQGVESVF
                     PMIYLRTGASVEYQRLQTYANIRGIDPQSLASMDFDLQGGAARLGNWQVVIGSRIAEN
                     FRDPRHPEELLEAPDLLGKTVELVVFGFRDPNQPNEKRVRVRVAGILEQGGRFDYTIF
                     LSLNETEDLNAWASGTGSNTRVDGYDTVYVQVSSPDRVDSVEDQINQSGFSAYSIQSQ
                     MRQLNLLFSAIQAVLGAVGGVALLVAGFGIANAMIMAIYERTREIGLMKAVGARNREI
                     MAIFLTEAGLIGMLGGIAGAIIGWLLGKGVGLIAQAYLRSVISAADTGGTAIPNLIYT
                     PLWLILFAVGFSALVGVISGLYPALRATRMDPIQALRYE"
     gene            complement(1830..>3460)
                     /locus_tag="VN363_07985"
     CDS             complement(1830..>3460)
                     /locus_tag="VN363_07985"
                     /inference="COORDINATES: protein motif:HMM:NF012376.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="bifunctional metallophosphatase/5'-nucleotidase"
                     /protein_id="HWQ84488.1"
                     /translation="AAPSSTLDVQILAINDFHGNLEPPAGSSGRIGTINAGGAEFLAT
                     HIQNLRATNPNTVVVSAGDLIGASPLISALFHDEPTIEAMNLIGLNINAVGNHEFDEG
                     VTELLRMQYGGCHPVDGCQDGDPFGGAKFSFLAANVVWADTGKPIFPAVRTIAVRDRV
                     KTKSGFTQITKKIAFIGMTLEGTPTIVTPSGVAGVRFLDEADTVNALVPRLKALGVEA
                     IVVLVHEGGFQAGTYNECVGIAGPIVDIVNRLDPEVDMVISGHTHQAYNCVINDKLVT
                     SAASFGRLVTDIDLTLDMQTGDITSMGANNVIVTRDVAPSSLLSTLIEKYRTLSAPLA
                     NRIIGAISADILRATNAAGESALGDVIADSQLYATAPAGFGEAVIAFMNPGGIRADLI
                     YAQSGSEGDGNVTYNEAFTVQPFGNSLVTMSLTGDQIRRVLEQQFDNPSVGSRRILQV
                     STGFTYAFSESAPTGSKIDPASIMLNGVPLDMNAVYRVTVNNFLADGGDNFSVLSEGT
                     DRLGGAQDLDALEAFLTAFSPVGPGPMDRIIQLP"
BASE COUNT          736 a          997 c         1003 g          724 t
ORIGIN      
        1 ggtgcttact ctgttagtca aaaatttacc tcaaatccag gtctcctatt accgcccgat
       61 tgagctggcg gaggtggagc gaccggtgga gctgcccatc gagatcatga atatcgggcg
      121 caccagtatc aacatcagcc aggcggaggt gaccgcggat caggtggaga tcgaaaatgg
      181 ctctgctttt atcggatccc tggatggagg cacatcggcc ttcctcgatg cagttggaat
      241 cccccagacc agcggtgatc tggagctgcg gctgaccgtg aattacctgg acgatttcaa
      301 ccagccgcat gtgatcactg agacactcac attaagtgtt gaagccccac ctgagcttcc
      361 ttccggcgcc aacggaatgc cgcctgagca ggaaggggga ttcgcccagg gtctgctgta
      421 ctttatccgc ggcctgtttg ggttgggaag ctagatgatg gattggcgca gcctgctgca
      481 tatcgtcctg cgcaacttga ggcggatgaa actgcgcgcc gcgatgaccg ccatgggggt
      541 cgtgattggc acgatcgcca tcatcttgct ggtctccatc ggcgccgggc tgcagcgttt
      601 tgccttccag ggagtagccg gttccaccga gctgaccctg attgaagtct cccagggaaa
      661 caatgggaat gccgccgtag ccagcgatcg cccgccggcc ctcctggacc ggcgcgcttt
      721 ggcagaattt cgcaacattc agggagtcga gagcgtcttt cccatgattt acctgcgcac
      781 tggagccagc gtggagtacc agcgcctgca aacctatgcg aatatacgcg ggattgaccc
      841 acagagcctg gcaagcatgg attttgatct acaaggcgga gcagcccgcc tgggaaactg
      901 gcaggttgtc atcggtagcc ggatcgctga aaatttccgg gacccacgcc atcccgagga
      961 gctcctcgaa gcgccagacc tgctcgggaa aacggtcgag ctggttgtat tcggcttccg
     1021 ggatccaaat cagcccaacg agaaacgggt acgggtgcgg gtggcaggta tcctcgaaca
     1081 gggtgggcgc ttcgattaca cgatttttct atcgctaaac gaaacggaag acttgaatgc
     1141 ctgggcttcg ggtaccggca gcaatacccg cgtcgatggc tatgacacgg tatacgtgca
     1201 ggtcagcagc cctgatcggg tggacagcgt cgaagaccag atcaaccagt ccgggttttc
     1261 tgcctactcg atccaatcgc aaatgcgcca gctcaacctg ctcttctccg ctatccaggc
     1321 tgtgctgggc gccgtcggcg gggttgcctt actcgtcgct ggcttcggca ttgccaacgc
     1381 catgatcatg gcaatctacg agcgcacccg tgagatcggc ctgatgaaag ctgtgggggc
     1441 gcgcaaccgg gagatcatgg caatcttcct gaccgaagcc ggattgatcg ggatgttagg
     1501 cggcattgcc ggggcgatca tcggctggct gctcggaaaa ggggtagggt taatcgccca
     1561 ggcgtatctc cgctcggtga tcagcgcggc tgatacaggg ggaacggcga tccccaacct
     1621 gatctacacc cccttatggc tgatcctgtt cgcggtgggc ttttcagccc tggtcggcgt
     1681 gatctccggt ctgtacccgg ctttgcgcgc cacgcggatg gacccaatcc aggcgctgcg
     1741 ctatgaataa atccggggct gccataaggt tcagcagccc cgggtaaaaa cctgcctgcg
     1801 atctgccggt caaccggcat ccatccgtgc tatggtaatt gaataatccg gtccatcggt
     1861 cccggaccaa ccggcgagaa cgcggtgaga aaagcctcca gggcgtccag gtcctgagcg
     1921 ccgcccaggc gatcagtgcc ttcacttagc acactgaaat tatcaccgcc atctgccagg
     1981 aaattattca ctgtcacccg gtagaccgca ttcatatcca gcggcacacc attgagcatg
     2041 atcgaagccg gatcgatttt cgagcctgtt ggcgcacttt cgctgaaggc atacgtaaat
     2101 ccagtggata cctgcaggat ccggcgtgag ccaacgcttg ggttgtcaaa ctgctgctcg
     2161 agcactcgcc ggatctggtc gccggtcagg ctcatggtca ccaggctgtt cccgaacggc
     2221 tgcactgtga aggcttcgtt gtaggtgaca ttcccatctc cctcgctgcc actctgggca
     2281 tagatcagat cggcgcggat accacccgga ttcataaagg cgatcactgc ttcgccaaaa
     2341 ccagccgggg cagtggcgta gagttgggaa tcggcaatca catcgcccag ggccgattca
     2401 ccggctgcat tggtcgcgcg caggatatcc gcactgattg cgccgataat ccggttcgcc
     2461 aggggggctg ataaggtgcg gtatttctca atcagcgttg aaagcagtga gctgggcgct
     2521 acgtcccggg tcacaatcac attattagca cccatgctgg tgatatcgcc ggtttgcata
     2581 tccagggtca ggtcgatatc cgtaaccaga cgcccaaagg aagctgcact ggtgaccagc
     2641 ttatcgttga tcacgcagtt ataagcctgg tgggtatgcc cgctgatgac catgtccacc
     2701 tcgggatcga ggcggttgac aatatccaca attggtccag caattccgac acattcattg
     2761 taagtgcctg cctggaagcc gccttcgtgc accaaaacca cgatggcttc gacgcccaat
     2821 gccttaagcc tgggcaccag agcattcacg gtatccgcct catccaggaa gcgcacgccc
     2881 gctactccgg agggagtgac aatcgttggg gtgccttcca gggtcatgcc aataaacgca
     2941 atcttcttag tgatttgcgt aaagccggat ttggttttaa cccggtcgcg gactgcaatg
     3001 gtacgcactg ccgggaaaat cggcttgccg gtatctgccc agacgacatt ggcagccagg
     3061 aaggagaatt tcgcccctcc gaaggggtcg ccatcctggc agccatcgac cgggtggcag
     3121 ccaccatact gcatccgcag cagctcggtg acgccctcat cgaattcatg gttgcccacg
     3181 gcattgatat tcaagccaat caaattcatc gcctcgatag tgggctcatc gtggaacagg
     3241 gctgagatca acgggctggc gccaattaaa tcgccggccg agactaccac cgtattcgga
     3301 ttagttgcac gcaaattctg gatatgagtt gccaggaatt ctgccccacc cgcattgatc
     3361 gtgccaatcc gaccggaaga accggcaggg ggttccaggt tgccgtgaaa atcattgatc
     3421 gccagaatct gtacatccag cgtgctggag ggagcggcag
//
LOCUS       DATMXK010000431         5657 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_4201, whole genome shotgun sequence.
ACCESSION   DATMXK010000431 DATMXK010000000
VERSION     DATMXK010000431.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5657)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5657)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5657
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4201"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1083
                     /locus_tag="VN363_07990"
     CDS             <1..1083
                     /locus_tag="VN363_07990"
                     /inference="COORDINATES: protein motif:HMM:NF015531.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008236 - serine-type peptidase activity
                     [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="S41 family peptidase"
                     /protein_id="HWQ84489.1"
                     /translation="TWNIPDAHVGLTFNAEIFYQEAGGSFGLRLAELSDGRVIVVEVI
                     PDTTGAEAGLVAGTEILTWDGKPVLDAMAEVVPYFGPYSTEQHKRLEQLVFLTRLPPD
                     TKVEIGYRLPDGQEETQELTAEVEYESLFRSLPELRSDPLSLPVSVSLLPDSGLGYIR
                     IDTFSDDYHLMAQLWEDAIQNLIDNEVPGLIIDIRSNGGGSSSLAYDFAGYLFDEEIV
                     LYQGAYYNERLGDFEFSDYPARIRPGPLYFEGPVAVLISPYCVSACEGFAYALSQGGR
                     ASIVGHYGTAGAFGEVGRGQYELPGEISMQFPTGRPETMDGQLLIEGAGVQPDIVVPV
                     TEASALEEVDAVLDTAVQAVQEQLGG"
     gene            complement(1172..2821)
                     /locus_tag="VN363_07995"
     CDS             complement(1172..2821)
                     /locus_tag="VN363_07995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84490.1"
                     /translation="MMSLLLPRHLTKWIICLSLAGLASACAPVSQPTVELPPEGTLPS
                     IETPARQKDPLQTSVASLESSPIQIAVQTEVSVKPSSTGASPTSLAAGGTVSIPAANN
                     TPASPTPPAVLAAITPRPPAEPIFGIELHDISEAGGLGLLTGAGGHWVRRNALKWSEV
                     EYLPGQRSWQNLSRLEAELEAASAAGLQTILIVRSTPHFARQSASSACGPVASEYFEA
                     FADFMAEAVSRYSQPPYNVRYWELGNEPDVDPSLVSSDSVFGCWGDQSDPDYGGQHYG
                     EMLKVVYPQVKAANPDAQLLIGGLLLDCDPDNPPETTPGSGKQKDCSSSRFLQGILEA
                     GGGAYFDAVSFHAYDYYAGELGLYGNSNWHSAWNTTGPALYAKTQFLTALLARYGWGD
                     KPLLNTEVALICASSPGVCASEEFMLTKAYYTAQANASALAAGLTANLWYSLQGWRDT
                     GLVGSGMTPNIAYTAYAASLERLSEAAYAGRLQPEDGLMGIAYDRQGTPGWIVWAWDG
                     AAHSLALPQTPARVFDVFGEPVEAASPLEIGVAPVYLEWQP"
     gene            complement(2831..4339)
                     /locus_tag="VN363_08000"
     CDS             complement(2831..4339)
                     /locus_tag="VN363_08000"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF024629.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 39 protein"
                     /protein_id="HWQ84491.1"
                     /translation="MSNQARYRLGFIFLLALLLVSQLSLASQRSFWEDEAATARLAAL
                     SAPEIIVERANNNHPPLYWLLVGLWGRFFGQDELGLRTFSILSLVGVLGLIYTLAGSL
                     YSPRAGWLAGLLLVASPYVLTYGHNARYYAFAAALSLLALLAAYRYLEEHSVWALVVY
                     MLAGTALIYTIYMGGTILVGINLWMLVETIRRKRSFRQYIVWLSIQVLVLLSYLPWLG
                     YLSGVVGRNLDPAYSLAGLPSAITLRLGYLGFAYCVGEFFSPFHPLVWLGILVSLLLL
                     ISAVRRLDAITSLLVSVLVVAVLTSVIVSSLSVFPQSAWQNLSNRTFFVYPCFILLLA
                     AGLDRLRVRRLQVGLALLLVVYLVGGVNVFTGRQAVKPLLIVPWVQVMEKIQIESRPG
                     AIVFCSHFDTVCPYYVDRFGLPRASLGGWEQVLASQPPEVWWLQNNIGGYDYDRALEE
                     AAFQALQAAYPQVKRLDYGAQDASIRSIKARWLGQEDYPYRLNVYHFSTAVP"
     gene            complement(4336..>5657)
                     /locus_tag="VN363_08005"
     CDS             complement(4336..>5657)
                     /locus_tag="VN363_08005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84492.1"
                     /translation="LIFALAGLVTRQTAYSAILLWPLAVLLAWYRQAKPGARKLAWAA
                     GGLLLLVGLGVLLARDDAAGWYRETLQAGPDRVETRAAPQGQYALRLTTLAGEAPGRL
                     VQILPYAAAGELAGKQVTLGAWIWADQPLEIRSPEIYVYARGKGYSRPISITTQPQFF
                     AFTIRLGRETHRTWVILSPGAGRPEETRNIYYDGLVLAEGAFPAGEPPVFTSTRADSG
                     TWGGVAFTNYLRNASAESAGLRVRPWVDGLGERLLPETGFERLSLTLYTVLDRPAAGY
                     YYLETFKNLGRTFWAVFGWNGIYLPGSNPYRPLAWITVLGLLGAMLAGWHYRRQLPWS
                     VILLFAVALIAVWGFAMLRGSNYIFMRLGFLPAARYVYPAVVPAMLAFTGGWVYLLEQ
                     SLGKWLRQTDRNHLAWLAYLPLGVFLAGLNIYCLVSIVRAWQSMGVL"
BASE COUNT         1261 a         1700 c         1674 g         1022 t
ORIGIN      
        1 acctggaaca tcccggatgc ccacgtcgga ctgaccttca acgccgagat cttctaccag
       61 gaagccgggg gcagctttgg cttgcgcctg gctgagctaa gcgatgggcg ggtgatcgtg
      121 gtggaagtga tccccgatac caccggagct gaagccgggc tggtcgccgg aactgagatc
      181 ctgacctggg atggcaaacc ggttttggat gccatggcag aggttgtacc ttacttcggt
      241 ccctattcca cggagcagca caaacgcctg gagcagctgg tctttttaac ccgcctgccg
      301 ccagacacca aggttgagat cggctaccgc cttccggatg ggcaggaaga aactcaggaa
      361 ctgaccgccg aagtggaata tgagtctctg tttcgttcgc ttcccgaact gcgcagcgat
      421 ccgctgtccc tgccggtcag cgtgagcctg ctgccggatt ccggcctcgg ttatattcgc
      481 atcgatactt tttccgacga ctatcacctg atggctcagc tctgggagga cgccatccag
      541 aatttgatcg ataacgaagt gcccggtctg attattgata ttcgctcgaa cggcggcggc
      601 tcgagcagcc tggcatacga ttttgccggc tatttattcg atgaagagat cgtcctctat
      661 cagggggcgt attacaacga gcgcctgggt gattttgaat tttctgatta tccggcgcgg
      721 atcaggcctg gccccctgta ctttgaaggt cccgtagcgg ttttaataag cccgtactgt
      781 gtcagtgcct gtgagggctt tgcatacgcc ctcagccagg gcgggcgagc gtcgatcgtc
      841 gggcattacg gtactgccgg ggcgttcggg gaggtaggcc gcggccaata tgagctgccc
      901 ggtgagatca gcatgcagtt ccccaccggt cgcccggaga cgatggatgg ccagctgttg
      961 atcgaaggcg caggtgtaca gccggatatt gttgtcccgg tcaccgaggc gtccgcattg
     1021 gaggaagtcg acgccgtgct ggatacggcg gtccaggccg tgcaggagca gcttggggga
     1081 taatccccaa cctgattaat taaactgcgc ctgtcgcatc gctgcttcag gcgcagtctt
     1141 tatgattaag aacgtttgga ctaagcggtt ctcagggctg ccattccagg tacaccggcg
     1201 caaccccgat ctcaagcggg ctggcagcct ccaccggttc accaaaaaca tcgaaaaccc
     1261 gggctggcgt ctgggggagc gccaggctgt gggcagctcc gtcccaggcc cagacaatcc
     1321 agcccggtgt accctggcgg tcataggcga tgcccatcaa gccatcttca ggctgcagtc
     1381 ggccggcata ggcagcctca gacaggcgtt ccaggctggc ggcataggcg gtataggcga
     1441 tattcggcgt catccctgaa cccaccagcc cggtatcccg ccagccctgc aggctgtacc
     1501 acaggttggc ggtcaggccg gctgccaggg cgctggcatt ggcctgggcg gtgtagtaag
     1561 ctttggtcag catgaattct tccgaggcgc ataccccggg agatgaagca cagatcaggg
     1621 caacctcggt attcaacaaa ggtttatcac cccacccata acgggctaac agcgcggtca
     1681 gaaattgggt tttggcatat aatgccggtc cggtggtatt ccaggcgctg tgccagtttg
     1741 agttaccata caatcccagc tcgccggcgt aataatcata ggcgtgaaag ctgaccgcgt
     1801 caaaataagc tcccccgcca gcttcgagaa ttccctgaag gaaccggctg gagctgcaat
     1861 ctttttgctt ccccgagcct ggcgtagttt ctggaggatt atccggatcg cagtccagga
     1921 gcagcccccc gatgaggagc tgggcgtccg ggttggcggc tttgacctgc ggataaacca
     1981 ctttaagcat ctccccataa tgctgcccac cataatccgg atcggactgg tccccccagc
     2041 agccaaacac gctgtcagag ctcaccagcg aggggtcgac atccggctca ttgccaagtt
     2101 cccaataacg aacgttatac ggcggctggc tgtagcggct gactgcttca gccataaaat
     2161 ctgcaaacgc ctcgaaatat tctgaggcga ctggcccgca ggcggagctg gctgactggc
     2221 gggcaaaatg gggtgtactg cgcacaatca ggatggtctg aaggcctgcc gcggaagctg
     2281 cctccagctc ggcttccagg cgtgacagat tctgccagct gcgctggccg ggcaagtatt
     2341 ccacctcgga ccatttcagt gcattacgcc gcacccaatg accacccgct ccggtaagca
     2401 agcctaaccc tcctgcctcg gatatgtcgt gcagctcaat cccaaaaatt ggctccgccg
     2461 gaggccgggg agtaatcgcg gccagaacag ccggtggcgt gggcgacgct ggagtattat
     2521 ttgcagccgg gattgacacc gtcccgccag ctgccaggct cgttggagat gcgcctgtgc
     2581 tggagggctt tacactgacc tcggtttgaa cagcgatctg gatgggactg gattccagag
     2641 aagcgacaga cgtttgcaaa ggatctttct gcctcgcggg agtctcgatt gaaggcaaag
     2701 tcccttccgg tgggagctct accgtgggct gcgaaacagg tgcgcatgca ctcgccagac
     2761 cagccaggct gaggcaaatg atccattttg taagatggcg cgggagaagt aagctcatca
     2821 ttaagcgacc tcaggggact gctgtgctga aatggtaaac attgagccgg tagggataat
     2881 cttcctgtcc cagccagcgg gctttgatgc tgcgaatgct ggcgtcctgg gcaccataat
     2941 cgaggcgttt cacctgcgga tatgccgcct gcagcgcttg aaatgctgcc tcttccaggg
     3001 cgcggtcata atcatagcct ccgatattgt tctgcagcca ccagacttcc ggtggctggc
     3061 tcgccagaac ctgctcccac ccacccaggc tggcgcgcgg caggccaaag cgatctacat
     3121 aataagggca gacggtatcg aaatggctgc aaaagacgat tgctcccggc cggctttcaa
     3181 tctggatctt ctccatcacc tggacccagg gcacaatcag gagtggtttg accgcctgcc
     3241 ggccggtgaa gacattcacc ccgccaacca ggtagaccac caggagcaaa gctaatccaa
     3301 cctgcagcct ccggacacgc aggcgatcca ggccagccgc caacagcaga atgaaacaag
     3361 gatagacgaa aaaggtgcgg ttggacaggt tttgccaggc agattggggg aaaaccgaaa
     3421 ggctgctgac gattacactg gtcagaacag ccacaaccag cacactgacc agcaggctgg
     3481 tgattgcgtc cagacggcgc acagccgaga tcagcaggag caggctgacc agaataccca
     3541 gccagaccag cgggtggaaa ggcgagaaaa actcacccac acagtaggca aaaccgaggt
     3601 agcccagacg cagggtgatg gctgatggca aacccgccag gctgtaggcg ggatccagat
     3661 tacgcccgac cacgccggag agatatccca accagggcag gtagctcaac aggaccagaa
     3721 cctgaatgga tagccagacg atatactgcc ggaaagatcg tttccggcgg atggtctcga
     3781 ccagcatcca caggttgatc cccaccagga tcgtcccgcc catataaatg gtgtagatta
     3841 aggctgttcc cgccagcatg taaacaacca gagcccagac ggaatgttct tccaggtatc
     3901 gataggctgc cagcagtgcc agcaggctta aagccgccgc aaaagcataa tagcgagcat
     3961 tgtgcccata tgtcagcaca tagggtgatg cgaccagcag caagcctgcc agccagccag
     4021 cccggggcga atacagcgaa cccgccagtg tatagatcag gccgaggacc ccgaccaggg
     4081 acaggatcga aaaagtgcgc agccccagct catcctgccc aaaaaaccgc ccccacagcc
     4141 ccaccagcag ccagtacagc ggcgggtgat tattgttcgc ccgctcgaca atgatctccg
     4201 gcgcgctcag ggcggccagg cgcgctgtgg cagcttcatc ttcccagaag ctgcgctggg
     4261 aggcgaggct gagctggcta accagcaaaa gggccagcaa aaagataaag cccagcctgt
     4321 atcgcgcttg attgctcaca gcacccccat ggactgccag gcgcgaacaa tgctgaccag
     4381 gcaataaata ttcaagcctg ccaggaacac gcccagcggt agataagcca gccaggccag
     4441 gtgattccga tccgtctggc gcagccattt ccccaggctt tgctccagca ggtagaccca
     4501 cccgcctgta aaagccagca tcgccgggac caccgccgga tagacatagc gggccgcggg
     4561 caggaatccc aggcgcatga aaatataatt tgagccgcgc agcatggcaa atccccacac
     4621 ggcgatcaga gccactgcga agagcagaat tacactccag ggcagctgcc ggcggtagtg
     4681 ccagcctgcc agcatagccc ccagcagacc cagaaccgtg atccacgcca gcgggcgata
     4741 gggattgctg cccggtaagt agattccatt ccagccaaac acggcccaga aggtccgtcc
     4801 caggttctta aaagtttcaa ggtagtaata acccgccgcc ggacggtcga gcacggtata
     4861 cagggtcagt gaaagccgct cgaacccggt ttcaggcagc aggcgctccc ccagcccatc
     4921 cacccagggg cgcacccgca ggccagccga ttctgccgag gcattccgca ggtaattggt
     4981 gaatgccacc ccgccccagg tgcccgaatc cgcccgggtg gaggtgaata ccgggggctc
     5041 gccggcagga aaagcgcctt ccgccaggac cagaccatca taatagatat tccgggtttc
     5101 ttcagggcgg ccagcgcccg gcgacaggat cacccaggtg cggtgggtct cgcgcccgag
     5161 gcgaatggtg aaagcaaaaa actgcggctg tgtggttatg ctgatcgggc ggctgtagcc
     5221 tttgcctctg gcatagacat agatttccgg agagcggatt tcaagcggct ggtctgccca
     5281 gatccatgcc cccagggtga cctgcttacc cgccagctcg cccgccgccg catagggtaa
     5341 gatctggacc aggcggcccg gagcttcccc ggctaaagtg gtcagccgca gcgcgtactg
     5401 tccctgagga gccgcgcggg tttcaacccg atccgggccg gcctgcagcg tctcccggta
     5461 ccaacccgcg gcatcatccc gggcaaggag tacacccaaa ccaaccaaga gcagcagccc
     5521 gccggctgcc caggccagct ttctggcgcc gggcttggcc tggcggtacc aggcgaggag
     5581 aactgccaga ggccagagca ggattgcgga gtaagctgtc tggcgggtga ccagacctgc
     5641 cagggcaaat atgagac
//
LOCUS       DATMXK010000432         4500 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_101240, whole genome shotgun sequence.
ACCESSION   DATMXK010000432 DATMXK010000000
VERSION     DATMXK010000432.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4500)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4500)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4500
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_101240"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..207)
                     /locus_tag="VN363_08010"
     CDS             complement(<1..207)
                     /locus_tag="VN363_08010"
                     /EC_number="3.5.4.16"
                     /inference="COORDINATES: protein motif:HMM:NF013398.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GTP cyclohydrolase I"
                     /protein_id="HWQ84493.1"
                     /translation="MELDEKRLLELAHLLPDDSVDQAAIQQVAKQMLSAFGEDPEREG
                     LLRTPGRVARMYEELLAGYRVDPIA"
     gene            376..819
                     /locus_tag="VN363_08015"
     CDS             376..819
                     /locus_tag="VN363_08015"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030551 - cyclic nucleotide binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cyclic nucleotide-binding domain-containing
                     protein"
                     /protein_id="HWQ84494.1"
                     /translation="MGLERMIRDIDLFHDLSQVEVREISALCRERVLQSGDYLVRQGE
                     YGDEMFILTEGFVEVLLERRGDTPPRVLITLGPGQTTGEMALLDLGPRSASVRAVSTP
                     TVVQAIQRAEFKALCEKNTQIGYKVMRNLALDLVFKLRHRNLLDG"
     gene            complement(925..1491)
                     /gene="raiA"
                     /locus_tag="VN363_08020"
     CDS             complement(925..1491)
                     /gene="raiA"
                     /locus_tag="VN363_08020"
                     /inference="COORDINATES: protein motif:HMM:TIGR00741.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_component: GO:0022626 - cytosolic ribosome [Evidence
                     IEA];
                     GO_function: GO:0045182 - translation regulator activity
                     [Evidence IEA];
                     GO_process: GO:0009386 - translational attenuation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome-associated translation inhibitor RaiA"
                     /protein_id="HWQ84495.1"
                     /translation="MTVEVDIFSRNMDVTDHIKDYVGKRVSKLDRFLGGIDEARVDLA
                     YVKSARSASDRQVAQITIRGKGFILRSEERGDDIFTAFDMALDKMQRRIERYKGKRNH
                     GRGDGRSTADVVPAPVIEEEPEDEAPVIMRRKRFPLSPMDENEALEQMSLLGHENFFV
                     FYNANTSQVNILYHRRDGSYGLIEPEIA"
     gene            complement(1584..2324)
                     /locus_tag="VN363_08025"
     CDS             complement(1584..2324)
                     /locus_tag="VN363_08025"
                     /inference="COORDINATES: protein motif:HMM:NF039185.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ComF family protein"
                     /protein_id="HWQ84496.1"
                     /translation="MKGSRFYPLYQLAWSSLDWLYPPSCGGCGLPGERWCPACRAAVR
                     ELKPPLCPHCGQMLAPDQVCLACPPGGYRFRALRAWAEFSGPLRGAIHRLKYNGDMAL
                     GESLARPLAGLLRGLAWRIDLVCPVPMGVARQQQRGYNQATLLALPLALACGLPYRPG
                     ALQKVRQTRTQVGLSRSARRQNVAGAFQAEARLVQGKTVLVVDDVATSGATLDACAAA
                     VLDAGAQAVYGLTLARAIYLPEGSAEAP"
     gene            complement(2379..3332)
                     /locus_tag="VN363_08030"
     CDS             complement(2379..3332)
                     /locus_tag="VN363_08030"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWQ84497.1"
                     /translation="MEWILFITLAVIIIGAVVVIVIGLRDSNREDKLQERLAEFAARG
                     ESATLEEIELSQPLSERVIVPLARSFGRFATRFTPQNAIQSTAHMLELAGNPRGLDPS
                     IFWLGRFFFAILLGGGLWWVFSVANSNWSLGQRIMVVVFAIIFGFFLPQLLLQARIDR
                     RQKSVRRAMPDALDLLTICVEAGLGFDGAMQKVFEKWDSELSLAFGRALREIQLGKTR
                     REALRSMADRLGVTEMTSFVAAVIQSEQLGVSMAKVLRIQSDQMRIKRRQLAEEEAHK
                     APIKMLIPMALLIFPSICIVLMTPSILMMMRSALSGILFGP"
     gene            complement(3348..4316)
                     /locus_tag="VN363_08035"
     CDS             complement(3348..4316)
                     /locus_tag="VN363_08035"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWQ84498.1"
                     /translation="MDPIFLILIGGGGLAVILLAVGVVFTLTSERSLVEQRLGRYMED
                     EARNAKKELDRSTVVSDWVNARLEKSQLGGGVARELAQADIKLRTAEYIIIVIFVGLL
                     MSAFIWYVGGRNIFVAIIGFLLGIMLPRMYVKRQQGLRLQHFNEQLADMLNLMVNGLR
                     AGYSTMQAIEAVSREMPSPISDEFWRVVQEMQLGISMEAALNNLMRRIPSDDLDLVVA
                     AMNVQREVGGNLAEILDTISYTIRERVRIKGEIRVLTSQVVYSGRFLAMMPVILGVAL
                     WFLNRPYMMEFFNPETRVVGLIALGIGAFMIVTGYFVMTRIAAIDV"
BASE COUNT         1003 a         1343 c         1258 g          896 t
ORIGIN      
        1 tgcaatcggg tccacccggt agccggccag taattcttca tacatgcgcg ccacccgccc
       61 gggggtgcgc agcaagcctt ctcgctcggg atcctcacca aaggcgctca acatctgctt
      121 cgcaacctgt tgaatagcag cctgatcaac actatcgtcg ggcagcaggt gggctaactc
      181 gagcagtctt ttttcatcca gttccatttc accccgcctt ttataaacca ggaattttga
      241 agggcaaccc cagaaatagt ttaaccgcgc cggtcgctcc cgtcaactgc agccaggcag
      301 ccagagatat ggagacggga ttgccttcaa cactgattaa cgctacaatt aagtcacccc
      361 agggaggtat cagggatggg actggagcgt atgatccggg atatcgatct attccatgat
      421 ttgagccagg tggaagtccg ggaaatcagc gctttgtgcc gtgagcgcgt tttgcagtcc
      481 ggtgattatc tggtccgcca gggagagtac ggagacgaga tgtttatcct caccgaaggc
      541 ttcgtcgaag tgctgttgga acgccgcggg gatacgcctc ccagggtgct cattaccctg
      601 gggccggggc agaccaccgg cgagatggcc ttgctggacc tgggaccgcg atctgcaagt
      661 gtgcgagcag tctccacgcc aaccgttgtc caggccatcc agcgtgcgga attcaaagcc
      721 ctgtgtgaaa aaaacactca aattggttac aaggtgatgc gcaacctggc gctggatctg
      781 gtctttaagc tccgccaccg caacctgctg gatggttagg aaaatcgccc gccctgattc
      841 acaacattca ttaatcgaaa cgtgggcggc tgttcaccgc ccacgttctg gtttaagtca
      901 gttcaggctg aggctggata ccggtcaggc aatctcgggc tcgatcaggc catagctgcc
      961 atcccgccgg tggtagagaa tattcacctg gctggtgttg gcattgtaaa agacgaagaa
     1021 attttcgtgt cccaacaggc tcatctgctc cagggcttcg ttttcatcca ttggagataa
     1081 cgggaagcgc ttgcggcgca tgatcaccgg ggcttcatct tccggttctt cttcgatcac
     1141 cggcgctggg actacatcgg ctgtgctgcg cccatccccg cggccgtgat tacgtttgcc
     1201 tttgtaacgc tcgatccgac gctgcatttt atcgagagcc atgtcaaaag cggtaaagat
     1261 atcatcgccg cgttcctccg agcgcaggat aaaaccctta ccccgaatag tgatttgagc
     1321 tacctggcga tccgaagcgc tgcgggcaga cttgacatac gccaggtcca cgcgggcttc
     1381 gtcaatcccg cccaggaagc ggtccaactt ggagacccgt ttccccacgt aatctttaat
     1441 gtggtcggtc acgtccatgt tacggctgaa gatatcgact tcgacggtca tctacaacct
     1501 cctctacttt gaatatagat cgggcgagtc gtgcgcccgt taatcagata tccgttttgg
     1561 ggctgacttg tcgatgagct gattcaaggc gcttctgcag acccttccgg gagataaatt
     1621 gcccgcgcca gggtgagacc gtaaactgcc tgggcgccag catccagcac agcggcggcg
     1681 caggcatcga gagtcgctcc actggtcgcc acatcgtcca ctaccagaac tgtctttccc
     1741 tgcacaagcc gggcttcggc ctggaatgca ccggccacgt tttggcggcg ggctgagcgg
     1801 gaaagcccca cctgggtgcg agtctggcgc actttctgta aagccccggg ccggtaaggc
     1861 aaaccacagg ccagagccag cggcaacgcc aggagtgtag cctgattata tccgcgctgc
     1921 tgctgtcggg caacccccat tggaaccggg cagaccagat caatcctcca cgccagacca
     1981 cgcagcagtc cagccagggg acgggccagg ctttcaccca gagccatgtc accgttatat
     2041 ttcaggcggt ggatggcacc gcgcagcggg ccgctgaact cggcccaggc acgcagggct
     2101 cgaaagcggt atccgcctgg cgggcatgcc aggcagacct gatccggggc aagcatctgt
     2161 ccacaatggg ggcaaagcgg gggtttgagc tcgcgcacag cggcgcggca ggccgggcac
     2221 caacgttcgc cggggagccc gcagcccccg cagctgggcg ggtacagcca atccaggctg
     2281 ctccaggcca gctggtaaag cggatagaaa cgggaccctt tcatgaccgg caaaggcacc
     2341 actgcctccc ggtgtgagcc gggtatccag agaacggatt acggtccaaa taagataccg
     2401 ctcaacgcgg agcgcatcat catcaggatc gagggtgtca ttaatacaat acagatcgag
     2461 ggaaagatca acaaagccat cgggatgagc atcttgatgg gggctttgtg ggcttcttct
     2521 tcagccagct ggcggcgctt gatgcgcatc tggtccgact ggatgcggag cactttggcc
     2581 atactcacac cgagctgctc actctggatc acggcggcga cgaagctggt catctctgtc
     2641 acacccaggc ggtctgccat cgaacgcagt gcctcccggc gagtcttgcc cagctggatc
     2701 tctcgcaggg cgcgcccgaa agccagggag agctctgagt cccatttttc aaagactttt
     2761 tgcatcgcac catcaaaacc caggccggct tccacacaga ttgtcagcag gtcgagagca
     2821 tcgggcatcg ccctgcggac agatttctgg cggcggtcaa tcctggcctg cagcagcagc
     2881 tggggcagaa agaaaccaaa aattattgcg aatacaacca ccattattcg ctggccgagg
     2941 ctccagttgg aattcgcgac tgagaatacc caccacaacc cgccgccgag cagaatggca
     3001 aaaaagaaac gccccagcca gaagatggat gggtcaaggc cgcgtggatt gccggccagc
     3061 tccagcatat gagcggtgga ttggattgca ttctgaggtg tgaagcgcgt ggcaaaccgg
     3121 ccaaaactgc gcgccagggg gacgatgacg cgttcactca gcggttggga gagctcgatc
     3181 tcttccagcg tggcgctttc accgcgggcg gcaaactccg ccaggcgctc ctgcagctta
     3241 tcttcccgat tagaatcccg taagccaatc acgatcacga caactgcccc aatgatgatt
     3301 accgccaggg tgatgaataa tatccattcc ataggaccag cctcttctta gacgtcaatc
     3361 gccgcaatcc gggtcatcac aaaataaccc gtcacgatca tgaatgcgcc gattcccagg
     3421 gcgatcaggc ctaccacacg ggtctcaggg ttaaagaact ccatcatgta agggcgattg
     3481 aggaaccaca gggcgacgcc gaggataacc ggcatcattg ccaggaagcg cccggagtag
     3541 acgacctggg aggtgagcac ccggatctcg cctttgatcc gcacccgctc gcggatggta
     3601 tacgagattg tgtccaggat ctccgccagg ttgccaccca cttcccgctg cacgttcatg
     3661 gctgccacca ccaggtccag gtcatcactg gggatgcggc gcatcagatt gttcagggca
     3721 gcctccatgg agatacccag ctgcatttcc tggaccaccc gccagaactc gtcggaaatc
     3781 ggagagggca tttcacgcga tacagcttct atcgcctgca tggtcgaata gcccgccctc
     3841 agcccgttga ccatcaggtt caacatatcc gccagctgtt cgttaaaatg ctgcaggcgt
     3901 aatccctgct ggcgtttcac atacatgcgg ggcagcataa tcccgagcag aaagccgata
     3961 attgcgacga agatattgcg cccaccaacg taccagataa acgccgacat cagcagcccg
     4021 acgaagatca cgataatgat atattcggca gtgcgcagtt tgatatcggc ctgggccagc
     4081 tcgcgagcga ccccgccgcc cagctgagat ttttccagac gagcattcac ccagtcggag
     4141 acaacggtgg atctatccag ttcttttttg gcgttgcggg cttcgtcttc catatagcga
     4201 cccagccgtt gttcgaccag ggaccgctca ctggtgaggg tgaataccac gccaacagcc
     4261 agcaggatga ctgccagacc gccgccgccg atcaagatta aaaagatagg atccatgcgc
     4321 tcaatccttg tcgagactca ctgctgatgc ccgcggcacc cttagtaccg gcgtcgctcg
     4381 ccaatcccga agaccgaggc cggcaggtga atacctgaag tctcgatttt atcgataaat
     4441 ttcgggcgca gcccggtggg gcgcagacgc ccgatgacct tattattctc gtacccggac
//
LOCUS       DATMXK010000433         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_107385, whole genome shotgun sequence.
ACCESSION   DATMXK010000433 DATMXK010000000
VERSION     DATMXK010000433.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107385"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..455
                     /locus_tag="VN363_08040"
     CDS             <1..455
                     /locus_tag="VN363_08040"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWQ84499.1"
                     /translation="RLRKPAAPQSNSRAWLLILGGLLLIGLTAAAIFLNQGPAAKVAA
                     SNPAAEISVAEAAEKYAAGVFLLDVRTPEEWTEVHIPNTTLIPLDELQARLSELPQNA
                     EIVVVCRSGNRSQQGRDILREAGFEQVSSMAGGIREWSSQGHPTVSGS"
     gene            complement(630..2285)
                     /locus_tag="VN363_08045"
     CDS             complement(630..2285)
                     /locus_tag="VN363_08045"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84500.1"
                     /translation="MKSVQRIAILILVVALAGLTLPSPAAIVNAQVSSSTTVYLPAIS
                     SQHSEAHSPFSIQIAGLSQITNDAAFANLTSKEAEITRENLIAELDAAFPSMLAAFEE
                     SGAGYARVYIDWKSIETTRGVYNWSFYDMRLPALHAAGLGLIATVTNAPDWAINPALD
                     PCAAIITEPQAYNQFLETMGNRFPFIDVWEILNEPDAIPGYRCGDGVMNYAYAGAQYA
                     SLLQGAYTTIKALNPTAKVILGGMAYDHFVDEDTAENRFYRYFLDDVINAGAAPYMDG
                     VNFHYFKNYAAGWEGWTNGNGSPTCEGIINVDEGGDTFYHPWGFDITAKASHVSERLR
                     VCHNVSKPLWLTEIGANGIGPNNSFYHYTQTPTEPFPHLYGATLEDQARYVFTIHARG
                     FATGAENITWYAMKTIPDLTPIDFQGLLYDARDGSALDNSPKPAFAAYKTMTRELGSY
                     RFDKWVAFDPATRPAAEAYQFQHLAGSAGGKPKLVAWSNSGSSVPLSLANSEIRVVSR
                     PDNGIFQPQFTVSDGGFGDADGVANGTIQFELQAEPVIIELVR"
     gene            complement(2415..>3110)
                     /locus_tag="VN363_08050"
     CDS             complement(2415..>3110)
                     /locus_tag="VN363_08050"
                     /EC_number="2.7.8.-"
                     /inference="COORDINATES: protein motif:HMM:NF014453.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_process: GO:0006486 - protein glycosylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar transferase"
                     /protein_id="HWQ84501.1"
                     /translation="LLMRAAALGAGLLSGFITPPRSPTRERPVLPMGPFFVKRAIDTV
                     GSLIGLVVSSPLILLSAIAIRLDSPGPAFFVQERAGENGKPFLIYKLRTMCADAEELV
                     ADVLVLNPLEGPTYKIPNDPRVTRVGRFLRRWSIDETPQFWNILRGEMSLVGPRPEEM
                     WIVEKYTDEQRKRLAFKPGLTGPMQVNGRGCLNFDERYQLELEYMRNYSLARDIDILI
                     RSLKAVITGKGAY"
BASE COUNT          725 a          850 c          850 g          685 t
ORIGIN      
        1 ccaggcttcg caaaccagcc gcgcctcaat ccaactcgcg ggcctggcta ttaatcctgg
       61 gaggtttgct gctcatcgga ttgaccgccg cagcgatctt ccttaaccag ggaccagccg
      121 ccaaggttgc cgccagcaat cccgcagcag agatcagtgt agccgaagca gcggagaagt
      181 atgctgccgg cgtgttttta ctggatgtac ggacacccga ggaatggacc gaggtgcata
      241 tcccgaacac caccctgata cctctggatg agctgcaggc ccgcctgagt gagttacccc
      301 agaacgctga aattgtggtt gtctgccgtt cggggaaccg cagccagcaa gggcgtgaca
      361 tcttgcgtga agctggcttc gaacaggtga gcagtatggc aggcgggatc cgtgagtgga
      421 gcagtcaagg gcatcccacg gtcagtggct cgtgagagca aacaccttgc gtttttgtca
      481 ggcgcttgct tgccattcga aatgaagaag ggtatactta tatgggcgtt gttggcctgc
      541 ccccctcagg tcagcaacgc ccctttttta accaaaaaac tggcgccagg acatgttccg
      601 gcgccagttg tgtttataaa aagccccgcc tatcgcacca gctcgataat caccggttca
      661 gcctgcagct cgaactggat cgtcccattc gctacaccat cggcgtctcc aaacccgccg
      721 tcgctgaccg taaactgcgg ttggaagatc ccattatccg ggcgcgagac gaccctgatc
      781 tcactgttcg ccaggctcaa tggcacactg ctgccggaat tactccaggc taccagcttg
      841 ggtttaccgc ccgcggagcc tgccaggtgc tggaactggt aggcttcggc tgccgggcgg
      901 gttgccggat cgaatgccac ccatttatca aagcgatagc tgcccagctc gcgagtcatc
      961 gtcttgtatg cagcgaaagc tggcttgggt gaattgtcca gcgctgatcc atcgcgagca
     1021 tcatacagca ggccctgaaa atcgatcggg gtcagatcag ggatcgtctt catggcatac
     1081 caggtgatgt tctctgcccc ggtggcaaat ccccgggcat ggatagtgaa gacatagcgc
     1141 gcctgatcct ccagcgttgc gccatataaa tgcggaaagg gttcggtagg ggtctgagta
     1201 tagtggtaaa acgaattatt cggaccgatc ccattggcgc cgatctcggt cagccataac
     1261 ggcttgctga cattgtggca aacccgcagc cgctcagaga catgcgaagc tttggcagta
     1321 atgtcaaaac cccagggatg gtaaaaagta tccccacctt catcgacgtt gatgatgcct
     1381 tcacaggtcg ggctgccatt tccattggtc caaccttccc aaccggcagc ataattctta
     1441 aagtagtgga agtttacccc atccatataa ggggccgctc cagcattgat cacatcatcc
     1501 aggaaataac gatagaacct gttctcagca gtatcctcat ctacaaagtg atcgtaagcc
     1561 atgcccccca ggatgacttt ggcggttgga ttgagggctt tgatcgttgt ataggcgcct
     1621 tgcagcaagg acgcatactg ggcgccggcg taggcataat tcatcacgcc gtctccacag
     1681 cgataaccgg ggattgcatc cggctcattg aggatttccc agacatcaat gaaagggaaa
     1741 cggtttccca ttgtctccaa aaactggtta taggcttgcg gttcggtgat aatcgcagcg
     1801 caaggatcca gggcaggatt gatcgcccag tctggtgcgt tggtcacggt agcaatcaga
     1861 cctaaccctg ctgcatgcag ggcaggcagg cgcatatcgt agaatgacca gttataaaca
     1921 ccccgggtgg tttcaatgct tttccagtca atatagactc tggcatagcc tgcgccactt
     1981 tcttcaaagg cagccagcat actgggaaag gcagcatcca gctcggcaat caggttttct
     2041 ctggtaattt cagcttcttt tgaggtcaga ttggcaaacg ctgcatcgtt ggtgatctgg
     2101 ctcagcccgg caatctggat ggaaaacggc gaatgcgcct ccgaatgctg gctggagatg
     2161 gcggggagat acaccgtagt ggaagagctg acttgagcat tgacgatcgc ggctggggac
     2221 ggcagggtta aacccgcaag cgccacgacc agaattaaga tcgcaattcg ttgtaccgat
     2281 ttcatgctaa ttaattatct ccttttggct gcccatatgt ataggcagca aacgggatgc
     2341 tatcaaacct taccgcagcc gtcaagtgcg atcgaattaa ggagctgtaa tgaatttcat
     2401 gaacgcgggg gaaattaata ggcgccttta ccggtaatca ccgctttaag ggagcggatc
     2461 aggatatcga tatccctggc aagtgagtaa ttgcgcatgt actccagctc cagctgataa
     2521 cgctcgtcaa aattcaggca gccgcggccg ttcacctgca tgggtccggt taaaccgggt
     2581 ttgaaggcca ggcgcttccg ctgctcatcc gtatattttt cgacaatcca catctcttca
     2641 gggcgcgggc cgaccaggct catttcgccc cttagaatat tccagaattg aggcgtctca
     2701 tcgatgctcc agcgccttaa gaagcggccc acccgggtga cccgcgggtc attgggaatt
     2761 ttataggtgg ggccttccaa tggatttaaa accagcacat ccgctaccag ctcttcggca
     2821 tccgcacaca tggtccggag cttataaatc aggaatggct tgccattttc gccggctctt
     2881 tcctggacaa aaaacgctgg accaggcgaa tccagccgga tggcgatggc ggagagcaag
     2941 attaatggag aggaaactac cagcccgatc aggctgccca ctgtatcgat ggcccgcttg
     3001 acaaaaaaag gacccattgg taaaaccggg cgttcgcggg ttgggctgcg cggcggcgtg
     3061 ataaaaccgc tcaacagtcc cgctcccagg gcagctgctc gcatcagcag
//
LOCUS       DATMXK010000434         2432 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_130938, whole genome shotgun sequence.
ACCESSION   DATMXK010000434 DATMXK010000000
VERSION     DATMXK010000434.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2432)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2432)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2432
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130938"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..678
                     /locus_tag="VN363_08055"
     CDS             <1..678
                     /locus_tag="VN363_08055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84502.1"
                     /translation="NLKTRVRPQTKLLTKAPLNITRIDTTTPQRQVVVVRDEIRLYTT
                     AGRDNQNRPKFVGAPPTKLHRGDQLLVYDQIRESQHDAGDGIIRGLDGEYVRISQSTV
                     PGATGKYLRRSDIEFMDQGQWMVFKAQSGTVGFETIKSYDTSRYKKPIFAPPATSPAS
                     LNLGQPGINRRVRVSTIHAESPLDMGDGVIQSTGLMFTHYLVLECPALPKARGYFIKT
                     DLVAREA"
     gene            complement(731..1066)
                     /locus_tag="VN363_08060"
     CDS             complement(731..1066)
                     /locus_tag="VN363_08060"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84503.1"
                     /translation="MANDLENSIRSAAEKIAAYVDNIATMTVETKYVRVDANGDADFS
                     QAKPVARTVIRIDGDSEAILPIRAGEDGGMAVDADLLDLHNQNVVTAIDYRAQLLNAL
                     IGMLKSSPR"
     gene            complement(1059..1811)
                     /locus_tag="VN363_08065"
     CDS             complement(1059..1811)
                     /locus_tag="VN363_08065"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84504.1"
                     /translation="MPESFDLGSAQNSGPAVFVCDLPANPEQAEFELAQAETALQSNL
                     ASLQDSQARLDQLVHIQAGGVAFDVPGYPPSPGYPPGPGYSPGSPESQLLALLGTSVP
                     DGEWDPSSFGAGEAVSGQWQKIAVGFQEFMDRLNQVVGHFAWVETQINGKLIVRSSVG
                     WTGNLDTAWNAAPTHESAALHQRTLRLALTSRTAMLRAFGVISAGAVKLTALVASPAG
                     AVLALPAAWNFIRQVQAESARYASIKQEIGNG"
     gene            complement(1915..>2432)
                     /locus_tag="VN363_08070"
     CDS             complement(1915..>2432)
                     /locus_tag="VN363_08070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84505.1"
                     /translation="AAQRPESISIPAMQSAATPPSPAAPLPAVTIPELQAVPAESLPA
                     DYGLMDSLAEARVRLTNAVNQFAESLGRTLTRVIDDASTLQVSTFVIDEMPARLGAEI
                     PADARLSAFTSIKIDGDTQVIVPSGSGGINHELWAIHLDMVEQAQTNRAEMMKAAASA
                     AAGLLQSLKGL"
BASE COUNT          522 a          724 c          709 g          477 t
ORIGIN      
        1 aacctgaaga ctcgcgttcg cccccagacg aagctgctta cgaaggcgcc cctcaatatt
       61 acccgcatcg ataccaccac cccccagcgc caggtggttg tcgtccggga tgagatccgg
      121 ttgtatacga ccgccggccg cgacaatcaa aatcgcccca aatttgtggg ggctccaccc
      181 acaaaattac accgcggcga ccagctgctg gtttacgatc agatccgtga aagccagcac
      241 gatgccgggg atggaatcat ccggggtctg gatggagaat atgtccggat cagccagtcc
      301 actgtgcccg gggcaaccgg aaaatacctg cggcggagcg atatcgaatt catggaccag
      361 ggccagtgga tggttttcaa ggcgcagagc ggcactgtcg gcttcgagac gatcaaatct
      421 tacgatacat cccggtataa aaagcccatt ttcgcaccac cagcgaccag ccccgccagc
      481 cttaacctgg gacagccggg gattaaccgg cgggtgcgcg tgtcgaccat ccatgctgaa
      541 agcccgctgg atatgggaga cggggtgatc cagtctaccg gattgatgtt tactcactat
      601 cttgtgttgg agtgcccggc cttacccaaa gcccgcggtt acttcatcaa aaccgatctg
      661 gtggcgaggg aagcctgaac ccctgccagg caggccatgc aatgtccctg cctggcaaac
      721 gccagatggg ctaacggggg gaagatttta gcattccgat gagagcattc aaaagctggg
      781 cgcggtagtc gatggcggta accacattct ggttatgcag gtcgagcagg tcggcatcga
      841 cagccatccc accgtcttca ccggcgcgga tgggcaggat cgcctcgctg tccccatcga
      901 tccggatgac cgtgcgggcg acaggttttg cctggctgaa atcggcatct ccgttcgcat
      961 ccacccggac gtattttgtc tccacggtca tcgtggcgat attgtcgacg taggcggcaa
     1021 ttttctcggc cgcgctgcgg atcgaatttt ccagatcatt agccatttcc aatctcctgt
     1081 ttgatagatg catatcttgc cgattccgcc tgtacctggc ggataaaatt ccaggcggct
     1141 ggcagcgcca ggactgcgcc ggccggcgac gcgaccagag cagtcagctt aactgccccg
     1201 gcagaaatca ccccaaaagc ccggagcatt gctgtgcgtg aggtcagcgc cagcctgagt
     1261 gtacgctggt gcagggctgc cgattcatgc gtgggggctg cattccaggc ggtatccaga
     1321 ttacctgtcc agccgacaga ggaacgcacg atcagctttc cattgatctg agtttctacc
     1381 caggcgaagt gaccgacaac ctgattcaag cggtccataa attcctggaa acccaccgca
     1441 atcttttgcc actggccgga taccgcttcc ccggccccaa agctggatgg atcccattcg
     1501 ccgtctggta cggaggttcc caacaacgcg agcagctggg attccgggct accgggagag
     1561 tagcccgggc cgggtgggta acccgggctg ggtgggtaac ccgggacatc gaaagcgaca
     1621 ccccccgcct gtatgtggac aagttgatcc aaccgggcct gggaatcctg gagactggcc
     1681 agattggact gcagggcagt ttctgcctga gcaagctcaa actcagcctg ctccggattg
     1741 gcgggcagat cacaaacaaa tacagcggga ccggaattct gagcgctgcc cagatcaaag
     1801 gattccggca aatcagtcga atcctgggca ggattccaga caccgaaaat atcccctgct
     1861 tgagcagcgc ggccggataa cacgaatgtg tttgtcccgc tggcagtcac cggcttacag
     1921 ccctttgagc gactgcagca gtccagccgc cgcgctggca gccgctttca tcatttcggc
     1981 gcgattggtc tgcgcctgct cgaccatatc cagatggatc gcccacagct catgattgat
     2041 cccgcctgag ccggaaggaa cgattacctg agtatcccca tcgatcttga tactggtaaa
     2101 ggcgctcagc cgggcatctg ccgggatctc agcgcctaat ctggccggca tttcatcaat
     2161 cacaaatgta gacacctgta aggtcgaggc gtcatcaatc acccgggtca gggtgcgacc
     2221 gaggctctcc gcaaactggt tgacggcgtt cgtcaggcgg acacgcgcct cggccagcga
     2281 atccatgagc ccgtaatcgg ctggcaggct ctctgctggt acggcttgca gctcgggtat
     2341 cgtaacagcc ggtaaaggcg cggcaggcga cgggggggtg gctgctgact gcatggcagg
     2401 aatcgagatc gattcgggac gctgtgcagc ct
//
LOCUS       DATMXK010000435         2502 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_53356, whole genome shotgun sequence.
ACCESSION   DATMXK010000435 DATMXK010000000
VERSION     DATMXK010000435.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2502)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2502)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2502
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53356"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(37..1515)
                     /locus_tag="VN363_08075"
     CDS             complement(37..1515)
                     /locus_tag="VN363_08075"
                     /inference="COORDINATES: protein motif:HMM:NF033551.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS1182 family transposase"
                     /protein_id="HWQ84506.1"
                     /translation="MLRPNASMEIPEDTKQTAKAAFPNGSLYLILRDKIGSLFEDEEF
                     MELYPSLGQPAESPARLALITVMQFLENLTDRQAANAVRSRIDWKYMLGLELGDPGFH
                     YSVLSEFRLRLVEGGAEKMLLDKLLERCKELDLLKGKKDQRTDSTHVLAAVRSLSLLE
                     LVGETMRRVLDEIARLSPEWLQEHLQLEWIKRYGRSFDSYRLPKNKEERQNLAVAIGQ
                     DGFYLLEEIYGETGPKELKTSPKMEVLRRIWVQQYYRRDGQVYWRTKEKWGQPPAGQM
                     ISSPDDPDIHYCVKRSTEWTGYKVHLTETYEPEQPRIITQVETTPSTVHDVKMTTQIQ
                     DELISKGLCPEIQLVDEGYMEIDLLVSSQKKGIDLVGPVPSSKNWQDREEGAYNHTCF
                     KIDWEQRVAICPQGKMSVHCTDRKTWRGTPSLTFVFDREDCLSCPVRKQCSRAKQAGR
                     TLTLYPQEQYEAQLEARKRRKRMSLKNYMESGLGLKALSLKA"
     gene            1678..>2502
                     /locus_tag="VN363_08080"
     CDS             1678..>2502
                     /locus_tag="VN363_08080"
                     /inference="COORDINATES: protein motif:HMM:NF033543.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004803 - transposase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="IS256 family transposase"
                     /protein_id="HWQ84507.1"
                     /translation="MTYQDDFTLPTELLEQVAEQGLDFLPELIRIVINTAMQVERQKH
                     LGVGPYERSVECQGHANGFKPKTVSTRIAPITFDIPQVREGGFYPQALEKGLRSERAL
                     KLALAEMYVQGVSTRKVAAITERLCGSEISSTQVSRASALLDEVLEAWRNRPLGTVIY
                     LYLDARYEKVRLDGQIRDAAVLMASGVNLEGKRLILGVSVSLGEQELHWRDFLQSLVE
                     RGLNGVELIISDAHVGLQAARKAVFSGIPWQRCQFHLQQNASQYVPRQSMKREVAAD"
BASE COUNT          560 a          671 c          616 g          655 t
ORIGIN      
        1 gcccaatata gcgggtattt ctcaagcctg cagttttcac gccttgagcg atagtgcttt
       61 caatcccagc ccgctttcca tataattctt taaactcatc cgtttcctcc tttttcgagc
      121 ttctaattga gcttcgtatt gctcttgagg gtaaagtgta agtgtccgtc ctgcttgttt
      181 ggctcgacta cattgctttc gcaccggaca ggacaaacaa tcctctcgat cgaaaacaaa
      241 tgttaggctg ggcgttcccc gccaggtttt tcggtcggta cagtgcacac tcattttccc
      301 ttgagggcat attgcaactc gctgttccca atcgattttg aaacacgtgt gattatatgc
      361 tccttcttct cgatcctgcc agttcttgct cgatgggacg ggaccgacca gatcaatccc
      421 tttcttctga ctgctgacca gaagatcaat ttccatgtag ccttcatcaa ccagttgaat
      481 ctccggacag agacctttgg agattagttc atcctggatt tgggtggtca ttttgacatc
      541 atgcaccgta gatggcgtgg tttctacctg ggtaatgatg cgtggctgct ctggctcata
      601 ggtttcagtt agatggacct tgtatcctgt ccattcggta ctgcgtttga cgcaataatg
      661 gatatcggga tcgtctggag aagaaatcat ctgcccagca ggtggctgcc cccatttctc
      721 cttcgtgcgc caataaactt gcccatcacg cctgtaatat tgttgcaccc agatcctgcg
      781 caggacctcc atcttcgggg aagttttcag ttctttgggt ccagtttctc catagatctc
      841 ttccaaaaga taaaatccat cttgaccaat cgcaacggca aggttctgcc gttcctcctt
      901 gttcttcggg agtcgataac tgtcaaagct gcgcccataa cgcttgatcc attctagctg
      961 caggtgttcc tgcaaccatt cgggggaaag ccgtgcgatc tcatctaata cccgccgcat
     1021 ggtctcacca accagttcta gcaaagagag tgatcgaaca gctgccaata cgtgggtcga
     1081 gtcggttcgt tgatcctttt tacctttgag caaatccagc tctttacagc gctcaagtag
     1141 cttatccagc agcattttct ccgccccacc ctcaaccaaa cgcagacgaa actcgctcag
     1201 gaccgaatag tgaaaacctg gatcacctaa ttccaagccc agcatgtatt tccaatcgat
     1261 ccggctgcgg actgcattag ccgcctgacg atctgtcagg ttttccagaa attgcatcac
     1321 tgtgatgagt gccaggcgtg caggactctc agccggttgt cccaaactcg gatataactc
     1381 cataaactct tcatcttcaa ataaagatcc gatcttgtct cttaagatga ggtagagact
     1441 tccgtttgga aaagccgctt ttgctgtttg ttttgtatcc tcaggaatct ccatagatgc
     1501 gttcggtctt agcatttcga ttcactcctt ttaccaataa ttctttgagg agtatatcaa
     1561 tttccgatca ttttctgact tcgccaacaa acacaactct tttctgtaaa aattagtttt
     1621 caaagaacag taggtcaggt gtatcctaaa ggttccgaca ccaagaagga gcacaccatg
     1681 acctaccaag atgattttac cttaccaacc gaactactgg agcaagtggc tgaacaaggc
     1741 ctggatttcc tgcctgagct gatccgcatc gtgatcaaca ctgccatgca agtcgagcgc
     1801 cagaagcatc tgggagttgg tccgtacgag cgctctgttg agtgccaggg acacgccaac
     1861 ggcttcaagc ccaagacggt gagcacccgg atcgcaccga tcaccttcga cattcctcag
     1921 gttcgagaag gcggcttcta cccacaggct ttggagaagg ggttgcgtag tgaacgggcc
     1981 ttgaagctgg cgctggccga gatgtacgtg cagggcgtgt ctactcgcaa ggtggctgcg
     2041 atcaccgagc ggttgtgcgg cagcgagatc tcctccacac aggtcagccg ggcgtctgcc
     2101 ttgctggacg aagttctgga agcctggcgc aaccggccgc tgggcaccgt catctacctg
     2161 tacctggatg cccgctacga aaaggtgcgg ctcgatgggc agatccggga tgcagccgtg
     2221 ttgatggcca gtggagtgaa cctggaaggc aaacgcctga tcctgggcgt gtctgtttca
     2281 ctcggtgagc aggagctgca ctggcgagac ttcctgcaaa gcctggtaga gcgcggcctg
     2341 aacggggtgg agttgatcat cagcgatgcc catgtggggc tgcaggcagc tcgcaaagct
     2401 gttttcagcg gtattccctg gcagcgttgc cagtttcact tgcagcagaa cgccagccag
     2461 tatgtgcctc gccagagcat gaagcgggaa gtggcagccg at
//
LOCUS       DATMXK010000436         2696 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_97135, whole genome shotgun sequence.
ACCESSION   DATMXK010000436 DATMXK010000000
VERSION     DATMXK010000436.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2696)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2696)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2696
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97135"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            101..544
                     /gene="mraZ"
                     /locus_tag="VN363_08085"
     CDS             101..544
                     /gene="mraZ"
                     /locus_tag="VN363_08085"
                     /inference="COORDINATES: protein motif:HMM:TIGR00242.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043565 - sequence-specific DNA binding
                     [Evidence IEA];
                     GO_process: GO:0051302 - regulation of cell division
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="division/cell wall cluster transcriptional
                     repressor MraZ"
                     /protein_id="HWQ84508.1"
                     /translation="MFFLGQYNHNLDDKGRLTIPARFRDTLTVEGAYIMQGFDSNLLV
                     LTEATFDELSQRLNQASLTDATARLLRRLLFSTASRADFDRAGRILIPQYLRETAGIN
                     ADAVVVGAGKYVEIWSAERWASQLQILQEPEANASRFEPYSILFS"
     gene            556..1533
                     /gene="rsmH"
                     /locus_tag="VN363_08090"
     CDS             556..1533
                     /gene="rsmH"
                     /locus_tag="VN363_08090"
                     /EC_number="2.1.1.199"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560723.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016434 - rRNA (cytosine)
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="16S rRNA (cytosine(1402)-N(4))-methyltransferase
                     RsmH"
                     /protein_id="HWQ84509.1"
                     /translation="MPGQTLGEDLPHQPVLYHEIIHALQPHHGGAYVDGTVGAGGHAW
                     GILHASQPDGLLLGLDLDPQALELARQRLAPFGNRVSLIQASYTTLTEQLSKTGWKGV
                     NGIVLDLGLSSMQLDTIERGFSFAADAPLDMRFNPQNPLNAADLVNKLPEQELANLIY
                     RYGEERRSRQIAKAIVQARPVSTTRQLAAIVLRASGPERRKTHIHPATRTFQALRIEV
                     NQELENLQSVLPQAISTLLPEGRLAIIAFHSLEDRIVKQYFHRESRDCICPPRQPVCT
                     CGHVATIKLITHSPIRPTDQETAQNPRARSAVLRIAEKLPARAAEDSET"
     gene            1569..2105
                     /locus_tag="VN363_08095"
     CDS             1569..2105
                     /locus_tag="VN363_08095"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84510.1"
                     /translation="MIVGSREPLVTLQEVIMVRGSNLQQAHSQAPWRIQRQVIGIVLV
                     AIIFGMLAAYAYLDISARSTAIGRHILTMQSAVEELQHINEDLSTQLALITASSVMET
                     RAKQMGFRSITTEETAFILVPEYYDRQPVQLAPQANLPVNPVSTRQIPAQYTESLFEW
                     IGRQARTPLFPTLMRVQP"
     gene            2102..>2696
                     /locus_tag="VN363_08100"
     CDS             2102..>2696
                     /locus_tag="VN363_08100"
                     /inference="COORDINATES: protein motif:HMM:NF015662.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84511.1"
                     /translation="MKNPSHSWRFSFLGIILSVFPLLILGRIISVQANPSQIEQLKAY
                     GDQFAWEPHNIIPARGQIFDRWGNLLAGNETVYEVGITLSEVRNPDTIALISSVVFGL
                     EYDQVKATAELEARPDQVYRVLTQSATQEQIDQYEIMTERVLQDSPKDKNENPPSLRG
                     LSYTKRLKRVYPEKQVGANILGFVNGDGIGYFGVEEYF"
BASE COUNT          650 a          739 c          696 g          611 t
ORIGIN      
        1 tcgaattcgt atatactgtg tgtacttatg ggctaaagtg ggagaaagtg gggcgccggg
       61 ataggccggc gctctgctgt taaaggatag gttagaacat atgtttttct taggccagta
      121 caaccacaac ctcgatgaca aaggccggct aaccattcca gcccggtttc gtgatacgct
      181 gacagttgaa ggcgcgtata tcatgcaggg tttcgattca aatttgctgg tactgaccga
      241 agccacgttt gatgagctct cccagcgctt aaaccaggcc agtctgacgg atgcaactgc
      301 ccgattgctt cgccggcttt tgttctcgac agccagccgg gctgatttcg acagagccgg
      361 acggatccta atccctcagt acctgcgcga aacagccggg attaatgccg atgctgtcgt
      421 cgtcggcgca ggcaaatatg tcgagatctg gtcggctgag cgatgggcga gtcagctcca
      481 gattctgcag gaaccagagg caaatgcaag tcgatttgag ccgtactcca tcctgttcag
      541 ctagcgctat ggattatgcc tggccagacc ctgggagaag acctcccgca ccaaccggtt
      601 ctttaccatg aaattataca cgctttgcag ccgcaccacg ggggggctta tgtggatgga
      661 actgtcggcg ctggaggtca tgcatgggga atcctgcatg ccagccagcc agatggactc
      721 ctcctggggt tggatctcga cccacaggcg cttgagcttg cccgccagcg tttggcaccg
      781 ttcgggaacc gggtcagcct tatccaggct tcttacacca cattaaccga acagttatca
      841 aagaccggct ggaagggggt aaatgggatt gtgctcgatt tgggattgtc gtccatgcaa
      901 ttggacacaa ttgagcgagg cttctccttt gcagcagatg ctcctctgga catgcgcttc
      961 aatccccaaa acccgctcaa tgcagccgac ctggtaaata agctcccgga gcaagagctc
     1021 gctaatctga tctaccgcta cggtgaagag cggcgctcac gccagatagc caaagccatc
     1081 gtacaagccc gtcctgtttc cacaacccgc cagctagcgg cgatcgtgct ccgggcgagc
     1141 ggtccagaac ggcggaaaac gcacattcat ccagccacgc gcaccttcca ggcgctcagg
     1201 atcgaggtga atcaggagct cgaaaaccta caaagtgtgt taccccaggc aatttctacg
     1261 ctcctgcctg aaggcaggtt ggcgatcatt gcctttcatt ctttagaaga tcgcattgtt
     1321 aaacagtact ttcatagaga aagccgggat tgtatctgcc cacctcgcca gcctgtctgt
     1381 acttgtggtc acgtggcgac aatcaagttg ataactcatt ctccgatccg cccaacagat
     1441 caggagaccg ctcaaaatcc tcgggcgcgc agcgcagtgc tcagaattgc tgagaaactc
     1501 cctgccagag cggcggaaga tagcgagacc tgaattggca cattagttgc aaacctgttg
     1561 gtaccagaat gattgttggc tcgcgcgagc cactggtaac cctgcaggag gtaatcatgg
     1621 tgcgaggaag taatttacag caagctcata gccaggcacc ctggcgtatc cagcggcaag
     1681 tgatcggtat cgttctggta gcgatcattt ttggcatgct ggctgcctac gcctacctgg
     1741 atataagcgc ccgctccact gccattggcc ggcatattct taccatgcaa agtgccgtgg
     1801 aagaattgca acacattaac gaagatttga gtactcagct ggcattgatc actgcttcga
     1861 gtgtgatgga aacccgggca aaacagatgg ggtttcgttc gataaccacc gaagagacgg
     1921 cctttatcct tgtaccggaa tattatgatc gccaacccgt ccagttggcc ccacaggcca
     1981 atctgccggt caatcccgtc tccaccagac agataccggc ccaatatacg gaatcgctct
     2041 ttgaatggat tggtcgccag gctcgcacac cactcttccc gacgctcatg agggtacagc
     2101 catgaaaaac ccttctcaca gctggcgttt ttcattttta gggattattc tttctgtctt
     2161 ccctctcttg atcctgggac ggatcatttc tgtgcaagcc aatccatccc aaatcgaaca
     2221 gctcaaggca tatggcgacc agttcgcctg ggaaccgcac aatatcatcc ccgctcgcgg
     2281 tcagatcttc gaccgctggg ggaatcttct ggctggtaat gaaaccgtct acgaagtcgg
     2341 cataaccctg tcggaagtac gcaaccccga cacgatcgcc ttgatcagca gtgttgtctt
     2401 tggtcttgaa tacgatcagg taaaagctac cgcagagctc gaagccagac ccgaccaggt
     2461 ttacagggtc ctcacccaat ctgcgactca ggaacaaatc gatcagtacg agatcatgac
     2521 cgagcgcgtc ttacaggaca gccccaagga taaaaacgaa aacccaccca gtttacgcgg
     2581 gctcagttat accaagcgtc ttaaacgtgt ctatcccgag aaacaagtcg gcgcaaacat
     2641 attggggttc gtcaatggtg atgggattgg ttactttggg gtagaagaat atttta
//
LOCUS       DATMXK010000437         2902 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_94866, whole genome shotgun sequence.
ACCESSION   DATMXK010000437 DATMXK010000000
VERSION     DATMXK010000437.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2902)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2902)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2902
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94866"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..386
                     /locus_tag="VN363_08105"
     CDS             <1..386
                     /locus_tag="VN363_08105"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ84512.1"
                     /translation="TEVGERGARLSMGQRQLVSLLRVLVHRPAIFILDEATASIDPFT
                     EWQIQQALNMILEYTTSILIAHRLSTVKAADRIIVLDHGKIIEEGNHTGLLAHGGHYA
                     ELYNTYFRHQSLDYIENARKWIQQD"
     gene            396..1604
                     /locus_tag="VN363_08110"
     CDS             396..1604
                     /locus_tag="VN363_08110"
                     /inference="COORDINATES: protein motif:HMM:NF024622.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4032 domain-containing protein"
                     /protein_id="HWQ84513.1"
                     /translation="MHHHSIDSEPPDFSDLPWHLPLEEWADKTSRLEELPRGESRHPV
                     VFVNYSSSLYAIKEMPGDLAESEYKLLVQLETANIQSVTPVGWRQSDYPTTPRRGLLI
                     TRYLDHSIPFRTLFRHGGLERYRKHLLDAIAGLLVQLHLAGVFWGDCSLSNTLFRRDA
                     GTLQAYLVDAETAEIHPKGLTPELRHLELEIMEENIGREMGELFFEAAQEGYHPVVTI
                     PPTDAGMYIRLSYQNLWEEVRKEIIIKPGENYRIQERIRTLNELGFSVSAVEMVELDS
                     GSQVILRPTVTDRTFHRDQLLSLTGQDTEEMQARKMMNEIMEIQGTLSTRENRSVPLN
                     VAAYHWLNQIYSPTVAYLRDLVDENTNEAELYCEVLENKWYLSEQAHHDVGHDAAAVD
                     YVRRFSTSSA"
     gene            1667..>2902
                     /locus_tag="VN363_08115"
     CDS             1667..>2902
                     /locus_tag="VN363_08115"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ84514.1"
                     /translation="MQSQGMFRRTVLYAGLVCLTVAAYLLLVSGFSLVVGSVIQPANP
                     WIFGLMLLVVGLVLYPMRARLQRLIDQLPWVRSAGHQIRLDEFESSISKAADLSELVG
                     LIRQTILAQLKPASVHIFLYDSPLNKYCAQADEQGQLTSDIQFSALSGLASHLARGEG
                     ILVIENPPLKGELSSDTTRLRLLGSQLFVALPGGERLTGWLALGPKGSGEAYTFSERN
                     YLKALAEQTAVVLSRVRVVADLERRVQELDVLTRVAQGVNITSNFDDLLELIYTQTSQ
                     IVPTRDFRVSVYDQRYSQLVLAFSVDNDVRISEREGKILQDGIGLDPEVLRSRRPVIT
                     TDYARECWARGLLPDLQGIYAWIGVPLNSAGETLGCISLGSRAPGMAYTPEQLLILQA
                     IADQAAGAMIKARLLRETEE"
BASE COUNT          673 a          721 c          786 g          722 t
ORIGIN      
        1 ataccgaagt aggtgagcgg ggagcgcgcc tatccatggg tcaacgccag cttgtctcgc
       61 tgctgcgtgt tcttgtccat cgtccggcaa ttttcatcct ggatgaagct acggccagta
      121 tcgatccctt tacggaatgg cagatccagc aggctctgaa tatgatcctg gagtacacca
      181 ccagtatcct gattgcccac cgcttatcga cggtgaaggc cgccgaccgg attattgtcc
      241 tggatcacgg taaaattatc gaagaaggca atcacaccgg attattggct cacggcgggc
      301 attatgccga attgtacaat acgtacttcc gccatcagag cctggattat atagagaacg
      361 cccgtaagtg gatccagcaa gattagagag cataagtgca ccaccactcg atcgattctg
      421 aaccccctga ttttagcgac ttgccctggc atttaccctt agaggagtgg gcggataaga
      481 cctcccggct cgaagaattg ccccgcggtg aatcacgcca tccggttgtt ttcgtcaatt
      541 actccagctc attatatgcg atcaaagaaa tgcctggcga cctcgccgaa tcggagtaca
      601 aactcctggt ccagctggaa accgccaata tccagagtgt caccccggta ggctggcggc
      661 agtctgatta tccaacaaca ccccgtcgcg ggctgctcat cacacgctac ctggatcatt
      721 cgatcccttt ccggaccttg ttccggcacg gtggtttgga acgctaccgg aagcatcttt
      781 tggatgcgat tgctggattg ttggtccagc tccatctcgc tggtgtattt tggggggatt
      841 gttcgttatc caataccttg ttccgccggg atgcaggtac gctgcaggct tacctcgtgg
      901 acgctgaaac cgctgagatc catcccaaag gcctcacacc cgagctgcgg catctggaat
      961 tggaaatcat ggaagagaat attgggcggg aaatgggcga attgtttttc gaagccgccc
     1021 aggaaggcta tcatccagta gtgaccattc ctcctacgga tgctgggatg tacatccgtt
     1081 taagctacca gaatttatgg gaggaagtcc gcaaagaaat cattatcaaa ccaggtgaaa
     1141 attatcgcat ccaggaacgg atccgcacat tgaatgaatt ggggttctcc gtcagcgcgg
     1201 tagagatggt tgagctggat tctggctctc aggtgattct gcgccccaca gtaaccgacc
     1261 gaacctttca ccgtgaccag ctcctcagtc tgaccgggca ggacactgaa gaaatgcagg
     1321 cccgcaagat gatgaatgaa attatggaaa ttcaggggac tctttcaacg cgtgaaaatc
     1381 gcagcgttcc cctcaatgtg gcagcctacc actggctaaa tcaaatctat tcgcccacag
     1441 tcgcttattt aagagatttg gtcgatgaga ataccaacga agccgagctg tattgtgagg
     1501 tcctcgaaaa taagtggtat ctttcggagc aggctcatca cgatgtcggt catgatgctg
     1561 cggctgtaga ttatgtaagg cggttttcga cctcctcagc atgacataac aaaacagtaa
     1621 actgaactgc attgctatcc atccgctggt aacgtacaat ttaagcatgc aatctcaagg
     1681 aatgtttcgc cggacagtcc tgtatgcagg attggtgtgt ctgacagtcg cggcttatct
     1741 tttgttggta agtggattca gcctggtagt ggggagtgtt atccagcctg cgaatccctg
     1801 gatctttggt ttaatgttgt tagttgtagg gctggtttta tatcctatgc gcgccagact
     1861 gcagaggctg atcgaccagt taccatgggt ccgatccgct ggccatcaaa tccgtctgga
     1921 tgagtttgag agctcaattt ccaaagctgc ggatctttcc gagctggttg ggctgatccg
     1981 ccagaccatt cttgctcagc taaagcctgc cagcgtgcat atctttctgt atgactctcc
     2041 acttaataag tactgtgcgc aggcggatga acaggggcag ttgacctccg acatccaatt
     2101 ctcagccctg agtggtctgg cttctcacct ggcgcgcgga gaaggcattc tggtaattga
     2161 aaaccctccc ctcaagggtg agctttccag tgataccacc cggctgaggt tgttgggcag
     2221 ccagctgttt gtcgctttgc ctggaggcga acgtctgact ggatggctgg cgttaggtcc
     2281 gaaaggatca ggggaggctt acacattctc agaacggaat tatctgaaag cccttgctga
     2341 gcagactgca gtggtgttaa gccgcgtcag agtggtcgct gatctggagc ggcgtgtgca
     2401 ggagctggat gttttgaccc gtgttgctca gggtgtcaat atcacctcga attttgacga
     2461 tcttttagaa ttaatctata ctcaaacaag tcaaattgtg cctacccgtg atttcagggt
     2521 gagtgtgtat gaccaacgct attctcagct ggtcctggca ttttctgtgg acaatgatgt
     2581 gcggatcagc gagcgtgaag gaaaaattct gcaagatggg atcggcctgg atccggaagt
     2641 tctccgttct cggcgtccgg taataaccac agattatgcc agagaatgct gggcgcgggg
     2701 cttattgccg gatttacagg ggatctatgc ctggataggg gtgccgctta atagcgccgg
     2761 agagaccctg ggatgcatca gcctgggcag tcgcgctccc ggcatggctt atacaccaga
     2821 gcagctgctg atcctgcaag caattgcgga tcaggctgcg ggagcgatga tcaaagcgcg
     2881 tttgctgcgt gaaaccgaag aa
//
LOCUS       DATMXK010000438         3746 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_76986, whole genome shotgun sequence.
ACCESSION   DATMXK010000438 DATMXK010000000
VERSION     DATMXK010000438.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3746)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3746)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3746
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76986"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /gene="malQ"
                     /locus_tag="VN363_08120"
     CDS             complement(<1..1397)
                     /gene="malQ"
                     /locus_tag="VN363_08120"
                     /EC_number="2.4.1.25"
                     /inference="COORDINATES: protein motif:HMM:NF011080.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004134 - 4-alpha-glucanotransferase
                     activity [Evidence IEA];
                     GO_process: GO:0005980 - glycogen catabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4-alpha-glucanotransferase"
                     /protein_id="HWQ84515.1"
                     /translation="MPDPTRQPGHTSSEPPDKTAKRASGILLHPTSLPGRFGIGDLGE
                     AAYHFVDFLADSRQQLWQIMPLGPTSYGDSPYQALSAFAGNPLLINLERLIEEHCLAP
                     WDFDGGPVFPEHQVDYGAVINFKQRLLHLAYANFKNNASPAQNAALAGFVEEQHTWLD
                     GYALFAALKDQHGGAPWNTWEPELAAHQAAALAQWGETHKDLVGYHQYAQFTFHQQWS
                     SLKAYANQKGIRIIGDIPIFVAFDSADTWANQDLFYCDSRGKPTLVAGVPPDYFSATG
                     QLWGNPLYRWEAMAQDGFAWWIARFRSALQQVDIVRLDHFRGFEAYWAVPADEKTAVN
                     GKWVKGPGEKLFRAIEQALGPVPIIAEDLGLITPEVEALREELGFPGMKVLQFAFSGD
                     PEHIYLPHNYEQNCVVYTGTHDNDTTLGWLSTASEQERWNVRQYLGGRGEDLNWELIR
                     LALMSVAGTAIFPLQ"
     gene            complement(1432..3417)
                     /locus_tag="VN363_08125"
     CDS             complement(1432..3417)
                     /locus_tag="VN363_08125"
                     /inference="COORDINATES: protein motif:HMM:NF023324.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004553 - hydrolase activity, hydrolyzing
                     O-glycosyl compounds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maltotransferase domain-containing protein"
                     /protein_id="HWQ84516.1"
                     /translation="MFPEEGRSRVIIEDVQPNIDAGRFPIKRILGEETRVEADILADG
                     HDALACALLYRKEGDENWSEVPMEPLLNDRWQASFTVQEPGIYLYTVQGWVDHFKTWA
                     RDLAKRVEAGQDVSVDLLIGAELIEKAARHAPPIDENRLTIFAKALRVGGEEGAKQAL
                     SPELAKLMAINGERRFVTTYDKTLQVVVDRERARYGAWYELFPRSCSPVAGKHGTFKD
                     VEARLPYVANMGFDVLYLPPIHPIGSSFRKGRNNTTSPGPDDPGSPWAIGSAEGGHKA
                     VHPELGTLADFRHLVEEAKKYDIEIALDIAFQCAPDHPYVSEHPEWFRRRPDGTIQYA
                     ENPPKKYQDIYPFDFETEGWRELWEELKSVFQFWVEQGVRIFRVDNPHTKPFPFWEWI
                     IPEIKRDCPEAIFLAEAFTRPKALYGLAKRGFTQSYNYFPWRNTRWELTQYLTELTQT
                     EVREYTGPNLWPNTPDILTEYLQFGGRPAFMTRFILAATLGPSYGIYGPAFELCENQP
                     LAPGREEYLDAEKYEIKAWDISRPDSLQGLIARVNQIRNENPAFRANHELRFHEVDND
                     QIIAYSKSSLDGTNQVLVVVNLDPHYKQSGFINLPLEELGIDSRQPYQAHDLLTEARY
                     LWQGTRNYVELNPMIVPAHIFVIRRKVRTEHDFDYYL"
     gene            complement(3509..>3746)
                     /locus_tag="VN363_08130"
     CDS             complement(3509..>3746)
                     /locus_tag="VN363_08130"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84517.1"
                     /translation="EVVVPDETGVLVDLALKPGTFEPVDAPKFTADLAQAINRVALDK
                     DLGEKFGKNGRRRVEVHFSWTAIAQRTMDLYRSL"
BASE COUNT          697 a         1136 c         1040 g          873 t
ORIGIN      
        1 tcctggagcg ggaagatcgc tgtcccggcg accgacatca gcgccaggcg gatcagctcc
       61 caattcaagt cctctcctct tccgcccaga tattggcgca cattccagcg ttcttgctca
      121 ctcgccgtgc tcagccagcc cagggtggta tcgttgtcgt gggtgccagt gtaaaccacg
      181 cagttctgct catagttgtg cggtaagtaa atgtgctccg ggtcgccgga aaaagcgaat
      241 tgcagcactt tcatccctgg aaagcccagc tcctcccgca gcgcctccac ctccggagtg
      301 atcaggccca gatcttcggc gatgatcggc accggcccca gggcctgttc gatcgcccga
      361 aacagtttct cccccggacc cttcacccat ttcccattga ctgccgtctt ttcatcggct
      421 ggcaccgccc aatacgcctc gaaacctcga aagtggtcga gccggacaat gtccacttgc
      481 tgcagcgcgg acctgaagcg tgcgatccac caggcaaacc cgtcctgcgc catcgcctcc
      541 cagcgataca gcggatttcc ccacagctgc ccggtcgcgc tgaagtaatc cggcggcacc
      601 ccggctacca gggtcggctt gccccgagag tcgcagtaga acaggtcctg gtttgcccag
      661 gtatctgcgc tgtcgaatgc tacaaagatc ggaatatccc cgataatccg gatgcccttc
      721 tggtttgcgt aagctttaag gctgctccac tgctggtgga aggtgaattg cgcgtattgg
      781 tggtatccga cgagatcctt gtgtgtctca ccccattgtg ccagcgccgc cgcctggtgc
      841 gcggcgagct ctggctccca ggtattccag ggtgccccgc cgtgctggtc cttcagcgcc
      901 gcaaacaggg cgtagccatc cagccaggtg tgctgttcct caacgaagcc tgccagggct
      961 gcgttctggg cagggctggc gttattttta aaattcgcgt aagccaggtg cagcaggcgt
     1021 tgtttgaaat tgatcaccgc tccataatcc acctggtgct cgggaaatac gggtccgcca
     1081 tcgaaatccc atggcgccag gcagtgctcc tcaatcaacc gctccaggtt gatcagtaat
     1141 gggttgccgg caaatgccga tagtgcctgg tagggagagt cgccgtagct cgtcggaccc
     1201 aacggcatga tctgccacag ctgctgccgg ctgtctgcca ggaaatctac aaagtgatag
     1261 gctgcttccc ccagatcacc gatcccaaaa cgccctggca aggaggtggg gtggagcagg
     1321 atgccgctgg ctcgcttggc agttttgtct ggtggctcgc tgctggtgtg accgggttgt
     1381 cgtgttggat cgggcatact gtttataacc tgttggttgc tgaatgagtc cctagagata
     1441 gtaatcaaaa tcgtgttcag tgcgtacttt ccgccggatc acaaagatat gcgccggcac
     1501 aatcatggga tttagctcca catagttgcg tgtgccttgc cacaagtagc gcgcttcagt
     1561 cagcaggtcg tgagcctggt acggctggcg ggagtctata cccagctctt ccagcggcag
     1621 gttgatgaac ccggattgtt tgtagtgtgg atcgaggttg accaccacca gcacctggtt
     1681 tgtcccatcc aggctgctct tggaataggc aatgatctgg tcgttgtcca cctcgtggaa
     1741 gcgcagctcg tgatttgctc gaaaggccgg gttttcgttc cggatctggt tcaccctggc
     1801 gatcaatccc tgcaggctgt ccggacggct gatatcccag gctttgatct catacttctc
     1861 tgcatccagg tactcctccc tgcctggagc caggggctgg ttttcgcata attcgaaggc
     1921 tggtccataa ataccatagc tcggtcccag ggtcgcggcc aggatgaacc gcgtcataaa
     1981 agccgggcgc cccccaaatt gcagatattc ggtcaggatg tccggggtgt tgggccacag
     2041 gttcggcccg gtatactcgc gcacttcggt ctgggtaagc tcggtcaggt attgggtcag
     2101 ctcccacctt gtattgcgcc atgggaagta gttatacgac tgggtaaagc cgcgctttgc
     2161 cagcccgtac agggctttcg ggcgggtaaa agcttctgca aggaaaatag cttccgggca
     2221 gtctcgcttg atctcgggga ttatccattc ccagaagggg aacggcttgg tatggggatt
     2281 atccacccgg aagatacgca ctccttgctc tacccagaac tgaaaaacgc ttttcagctc
     2341 ttcccacagc tcgcgccagc cctcagtctc gaagtcgaat gggtatatat cctggtattt
     2401 tttcggtggg ttctcggcat actggatcgt cccgtcgggt cgccggcgga accattccgg
     2461 gtgttcactc acataaggat ggtccggagc gcactggaag gcgatgtcga gcgcgatctc
     2521 aatatcatac tttttcgctt cctcgaccag atggcggaaa tctgccagcg ttcccagctc
     2581 ggggtggacc gctttgtgcc caccttccgc cgatccaatc gcccacgggc tgcccgggtc
     2641 gtctggcccc ggactggtcg tgttattgcg ccctttccgg aagctgctgc cgatggggtg
     2701 aattggcggc aggtagagca catcgaatcc catgttggct acatacggca ggcgcgcctc
     2761 cacatcttta aaggttccat gcttcccggc caccggcgag catgagcgcg ggaacagctc
     2821 ataccacgcc ccatatcgcg cccgttcgcg atccaccacc acttgcagcg ttttgtcata
     2881 agtcgtgaca aaccggcgct caccgttgat cgccatcagc ttcgccagct cgggggaaag
     2941 cgcctgtttt gcgccctctt caccgcccac ccgcagcgcc ttagcaaaga tcgtcagcct
     3001 gttttcgtct atcggagggg cgtgcctggc agctttttca atcagctctg ccccaatcag
     3061 gaggtcgacg ctgacatcct gcccggcttc gacgcgcttc gccaggtcgc gcgcccaggt
     3121 cttaaagtgg tccacccaac cttgcactgt atagagatag atccccggtt cctggactgt
     3181 gaacgaagcc tgccagcgat cgttaagcaa tggctccatt ggaacctcgc tccagttttc
     3241 atcgccttcc ttgcggtaga gcagggcgca ggccaatgcg tcgtgcccat cggccaggat
     3301 atcggcttct acccgcgttt cttcacccag aatgcgcttg atcgggaacc ttcctgcgtc
     3361 gatgttgggt tgcacgtctt caatgatgac ccggctgcga ccttcttcag ggaacatggc
     3421 cttcctccag aaattttgat ggttgctttc gcgctttaac gctcgtctgg cagcacttgc
     3481 ctggtggtct gttaattacc ccaggcgttt aaagtgatcg gtataaatcc atcgttcgct
     3541 gggcgattgc cgtccagctg aagtgaacct caacccgccg gcgcccgttc ttcccaaact
     3601 tctcacccag atctttatcg agcgctaccc ggttgattgc ctgggctagg tcggctgtaa
     3661 atttgggcgc atcgaccggc tcgaatgttc ccggcttgag cgccagatcg accagcacgc
     3721 cggtttcatc gggtacaacc acttcc
//
LOCUS       DATMXK010000439         5164 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_36247, whole genome shotgun sequence.
ACCESSION   DATMXK010000439 DATMXK010000000
VERSION     DATMXK010000439.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5164)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5164)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5164
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_36247"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..930)
                     /locus_tag="VN363_08135"
     CDS             complement(<1..930)
                     /locus_tag="VN363_08135"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558529.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadA"
                     /protein_id="HWQ84518.1"
                     /translation="MSKNRSEYVCQQCGRRFARMMGRCPNCNAWNSLVEEMVTPAVAA
                     AVRPLGGGRSTPRRLSEIDGDVDQRMPVSISEFGRVLGGGVVPGSVVLIGGDPGIGKS
                     TLLLQVALEIASRQKVLYVSGEESERQIKMRALRLLRSTEADAPEPALPDQLYLVTET
                     NLGVIEGHLQQVRPDLLIVDSIQTTYLDDMDSSAGSVSQVRECASRLREIAKSSSMAV
                     FLIGHVTKEGAIAGPRVLEHIVDTVLYLEGDRFQSYRLLRSVKNRYGATSEVGVFEME
                     ERGMVEVTNPSEAFLAERMINAPGSAIAVTMEGT"
     gene            complement(1014..3503)
                     /locus_tag="VN363_08140"
     CDS             complement(1014..3503)
                     /locus_tag="VN363_08140"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559818.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-dependent Clp protease ATP-binding subunit"
                     /protein_id="HWQ84519.1"
                     /translation="MERFTQRARRVLSLAHQEAERMRHSYIGTEHLLLGLIREEGGVA
                     GRVLRELGLEADRVQEMVERLTQPGDYQGTKLDLSPGTQQVLEYAVEEARRMGHHYIG
                     TEHLLLGLVRYGEGVALDVLRKLGVTPEQIRRQTRRVLQEGGSASQAAKAETAKTASK
                     SEAAKPKTPMVDQLATDLTSLAEEGKLDPVIGRQMEIERVIQILARRTKNNPALMGEP
                     GVGKTAIVEGLAQRIVEGDVPGPLLDKRVLQLDVGSLVAGTMYRGQFEERLKRVIDEL
                     KASGAILFIDEVHMLVGAGAAGSSVDAANILKPALSRGELQVIGATTLDEYRKHIESD
                     AALERRFQPVMVEEPSVDETIEILRGVRPAYEEHHRLTISDEALDAAAHLSSRYVTER
                     FLPDKAIDLIDESSSRVRMYKSPAAKAAKEMMGKLREARQNYALTVEDGRTDDAQEIQ
                     EQIDGLDRQLERLRSTWDRANSPVVSADDIAEVVSMWTGVPVMQIAQEESQRLMQMEE
                     ELHKQIVGQEEAIGSISRAVRRARAGLKDPRRPIGSFIFLGPTGVGKTELTKALARFL
                     FGSEDALIQLDMSEFMERHTVSRLVGAPPGYVGYEEAGQLTEALRRRPYSIVVFDEIE
                     KAHPEAHNMLLQIMEEGHLSDARGHKVDFRNAIIVMTSNIGADMIKRQATLGFALKQD
                     EDTEERLAYDEMRKKLTDSLKRVFRPEFINRLDSVIVFRSLNKQDIQQIVELEVAKVG
                     KRLEENQIQLEITPAALDMIADLGYDPDMGARPLRRVIQQKVEDPLSDALLSGQFKDG
                     DSILVDVKDGEVFLHSKREEVPEPPEAIAAA"
     gene            complement(3804..>5164)
                     /locus_tag="VN363_08145"
     CDS             complement(3804..>5164)
                     /locus_tag="VN363_08145"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWQ84520.1"
                     /translation="IIATIRENEILVSVGTKSEGVISGRELEQIPEQERATFHIGQEI
                     VVYVVASEDANGNVVLSYTRAREERDWEKVEKLKATEEIYHSKVVGYNKGGLIVPVGG
                     LRGFVPASQVSLVRRSGATGETPEQRWGKMVGESVDIRVIEVDRERRRLILSERQALQ
                     ETRETLKERLLDQLAEGDIRTGRVTSLAEFGAFVNIDGADGLVHLSEISWDRIQHPNE
                     VLSVGQEVKVKVISVDQERKRIGLSIRQLLRDPWLTRVEHLKEGMLIEGKITHLTKFG
                     AFARLDEELEGLIHISELSEQRVTHPKEVVHEGDVVTLRVIKVDPERRRIGLSLRKVD
                     SAAYADFDWKMALAEEVGKVGEEETPAAVSSAPTEEVPVDEVPVDETPVKEAAVEEAP
                     VEEAPVEGMPVEEMPAEEAPVEEAPVEEAPVEEVPVEEVPVEEAPAEEAPVDEDAPPA
                     EE"
BASE COUNT          996 a         1681 c         1287 g         1200 t
ORIGIN      
        1 cgttccctcc atcgttacgg ctattgccga gcctggggca ttgatcatcc gctcagcgag
       61 gaaagcctcc gagggattgg tcacttcaac catgccccgc tcctccattt cgaaaacccc
      121 cacttccgag gttgcaccat agcggttctt cacagagcgc agcaggcgat aggattgaaa
      181 gcgatcccct tccaggtata aaaccgtatc tacgatatgc tccagcaccc gcggtccggc
      241 aatcgcccct tctttggtca catgcccgat caaaaatacc gccatgcttg agcttttagc
      301 gatctcccgc aagcgcgagg cgcactcgcg cacctgcgat accgagccgg cggacgaatc
      361 catatcatcg agataagtgg tctggatcga atcgacgatc agcaaatcgg gtcgaacctg
      421 ttgaagatgc ccttcaatca cccccaggtt cgtctccgta accaggtaga gctggtcggg
      481 cagggctggc tctggtgcat cggcctccgt gctccgcagt aaacgcaggg cccgcatctt
      541 gatctggcgc tcggattctt ctccggagac atacaggact ttctggcggg atgcgatctc
      601 cagggcaacc tgcaggagca gggttgattt tccaatcccc ggatcgccgc cgatcaaaac
      661 aaccgagcct ggcacaacgc cgccgcccaa tacgcgccca aactcgctga tcgacaccgg
      721 catccgttgg tctacatccc catcgatctc tgatagccgg cgcggtgtcg accttccgcc
      781 gcccaacggg cgcaccgcgg cagctacggc cggcgtgacc atttcctcca ccagactgtt
      841 ccaggcgttg cagtttgggc agcgacccat catccgggca aaacggcgcc cgcattgctg
      901 gcatacatat tcgcttcgat ttttgctcat gggctaatta taaatggggt ttaccagccc
      961 ggtgtggtct gataaacccc atctcatcct tctatctggc ttacaacatt cgatcaggca
     1021 gccgcaatcg cctccggtgg ctcagggact tcttctcgct tggaatgaag gaagacctcc
     1081 ccatctttta catccaccag gatggaatcc ccgtctttaa actgccccga gagcagtgcg
     1141 tcggacagcg gatcttccac cttctgctgg atcacccggc ggagtgggcg agcgcccata
     1201 tccgggtcgt aacccaggtc ggcgatcata tccagagctg caggagtgat ctccagctgg
     1261 atctggttct cttccaaacg cttcccaact ttagctactt caagctcaac gatctgttgg
     1321 atatcctgct tgttcaggga gcggaagacg atcaccgaat ccaggcgatt gataaactca
     1381 ggccggaaaa cccgcttgag cgagtcggtc agcttcttgc gcatctcatc atacgccagc
     1441 cgttcttcgg tatcctcgtc ttgcttaagc gcaaagccca atgttgcctg gcgtttgatc
     1501 atgtcagcgc caatattgga ggtcatcaca atgatcgcgt tgcggaagtc caccttatgc
     1561 ccgcgggcat ccgaaagatg gccttcttcc atgatctgca gcagcatatt gtgtgcttct
     1621 ggatgcgcct tctcgatctc atcgaacacc acaatcgagt atggtcgccg ccgtaaagct
     1681 tcggttagct gtccggcttc ctcataccct acataaccag gaggggcgcc aaccaggcga
     1741 gagacggtgt gccgttccat gaactcggac atgtccagct ggatcaaggc atcttcgctc
     1801 ccgaacagga accgcgccaa cgctttggtc agctctgtct tcccgacccc ggtagggcct
     1861 aagaaaatga acgagccaat cgggcggcgt gggtctttaa gccctgcccg cgcccggcgt
     1921 acagctctag aaatcgaccc gatcgcctct tcctgaccaa cgatctgctt gtgcagttct
     1981 tcttccattt gcatcaagcg ctgcgattct tcctgggcga tctgcatcac cggcacgccg
     2041 gtccacatcg agaccacttc ggcaatatca tccgctgata cgaccgggct gtttgctcga
     2101 tcccaggtac tgcgcagccg ttcgagctgc cggtccaggc catcaatttg ctcctggatc
     2161 tcctgagcgt catcagtgcg cccatcttca acggtcagcg cataattctg gcgcgcctcc
     2221 cgcagtttac ccatcatttc tttggctgct ttcgctgccg ggctcttata catccgcacc
     2281 cgcgatgacg actcatcaat caggtcgatc gccttgtccg gcaaaaagcg ctcagtgacg
     2341 taccgggatg acaggtgggc tgccgcatcc agggcttcgt ccgagatcgt caggcggtgg
     2401 tgctcctcat aagccgggcg caccccccgc aagatctcga tggtctcatc cacagacggc
     2461 tcttcaacca tcactggctg gaaacggcgt tccagggcag cgtccgattc gatatgcttg
     2521 cgatattcgt ccagggtcgt tgcgccgatc acctggagct caccgcgtga gagcgcaggc
     2581 ttgaggatat tggccgcatc cacagacgat ccggctgctc cagcgcccac cagcatgtgc
     2641 acttcgtcga taaacagaat cgccccagac gccttaagct cgtcaatcac ccgcttgagg
     2701 cgctcttcaa actggccgcg gtacatcgta cctgccacca gcgagccgac atccagctgc
     2761 agcacgcgtt tatccagcaa ggggcctggc acatcgcctt ccacaatccg ctgcgccagc
     2821 ccttctacga tcgcagtctt gcctacaccc ggctcgccca tcaatgccgg gttattcttg
     2881 gtgcggcggg ccagaatctg gattacccgc tcgatttcca tctgtcgccc gatcaccgga
     2941 tcgagcttgc cttcctccgc cagggaggtc aaatcagttg ccagctggtc caccatcggc
     3001 gtcttgggtt tagctgcttc tgatttgctc gctgtcttgg ctgtctcggc tttagcagcc
     3061 tggctggctg agccgccttc ctgcagcact cgccgggttt gccgccggat ttgctccggg
     3121 gtgaccccca gcttacgcag cacatccagg gctactccct cgccatagcg caccagaccc
     3181 agcagtaaat gttctgtccc gatatagtgg tgccccatcc ggcgggcttc ttcaaccgca
     3241 tactccagca cctgctgggt gcccggcgaa agatcgagct tggtgccctg gtagtctccc
     3301 ggttgggtta accgctctac catctcctgc acccggtctg cttccagacc cagctcgcgc
     3361 agcacacgcc cggcgacgcc cccttcttct cgaattaatc ccaggagcaa atgctctgta
     3421 ccgatatagc tatgccgcat ccgctcagct tcctgatgtg ccaggctcaa cacccggcgt
     3481 gctctctgtg taaatctttc catgcctgac atggtacttc ctcctaccgt ttatctgcag
     3541 taccaccata tctacgataa tagtgttacc aggttgtgcc tgctcctggt cagggatacg
     3601 ccgcttcgat tgagaggcat tttatcccat tggattctac cagaatttag agtgggcatg
     3661 tctttttcaa acttgcaaac cagggttgtt aatacaactc ttagagataa gtagttccac
     3721 ctttactgtt gtatataagc aaggcgcacc cggtctaggt gcgcctcgat gttggtttta
     3781 ttgtagcact gcctggttgg cggttattcc tcagccggcg gagcgtcctc gtctacgggc
     3841 gcttcttccg caggtgcctc ttctacagga acctcttcta caggaacctc ttctacgggc
     3901 gcctcttcta cgggcgcctc ttctacaggc gcctcttccg caggcatctc ttctaccggc
     3961 atcccttcta ccggcgcctc ttctaccggc gcctcttcta ccgccgcctc ttttaccggc
     4021 gtctcgtcaa caggtacctc gtctacagga acctcttcag ttggagcaga gctgactgcg
     4081 gcgggagttt cttcttcgcc aaccttccct acctcttcag ccagggccat cttccagtcg
     4141 aaatctgcat aggctgcaga atcgaccttg cgcaggctca agccaatccg gcgccgttcg
     4201 gggtcaacct tgatcacccg cagggtgacc acatcacctt cgtgcacgac ttccttggga
     4261 tgggtaaccc gttgttcgct cagctcggaa atgtggatca aaccttccag ctcctcgtcc
     4321 aatctggcga acgcaccaaa cttggtcaga tgggtaatct tgccctcgat caacatccct
     4381 tctttcagat gctcaacccg ggtcagccat ggatcgcgca gcaattggcg gatcgaaagc
     4441 ccgattcgct tgcgctcctg gtctacactg atcaccttaa ctttgacttc ctgtccgacg
     4501 ctcagcacct cgttcgggtg ctggatgcga tcccaggaaa tctccgagag atggacaagc
     4561 ccatccgccc cgtcgatatt tacgaatgcc ccgaattcag ccaggctggt cacccgcccc
     4621 gtgcgaatat cgccctcggc gagttggtcc agtaaacgct ccttgagggt ctcgcgggtc
     4681 tcttgcaggg cctggcgctc ggataggatc agccggcgcc gctcgcggtc cacttcaata
     4741 acccggatgt cgaccgattc tcccaccatc ttgccccagc gctgttcagg agtctcgccc
     4801 gtcgcgccag aacggcgcac caggcttacc tgcgaggctg gaacaaaacc gcgcagccct
     4861 ccaacaggaa cgatcagacc gcctttgttg tagcccacaa ccttactgtg atagatttcc
     4921 tcagtggctt tgagcttttc caccttctcc caatcccgtt cttcacgcgc ccgggtatag
     4981 gagagcacga cattaccatt cgcgtcttca gaagcgacca catatacgac aatctcctgc
     5041 cctatatgga acgtcgcccg ttcttgctcc gggatctgct ccagctcgcg gcctgagatc
     5101 acgccttctg atttggtgcc aacgctgacg agaatttcgt tctcgcggat ggtggcgata
     5161 attc
//
LOCUS       DATMXK010000440         2827 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_35065, whole genome shotgun sequence.
ACCESSION   DATMXK010000440 DATMXK010000000
VERSION     DATMXK010000440.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2827)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2827)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2827
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35065"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            67..846
                     /locus_tag="VN363_08150"
     CDS             67..846
                     /locus_tag="VN363_08150"
                     /inference="COORDINATES: protein motif:HMM:NF016764.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YIP1 family protein"
                     /protein_id="HWQ84521.1"
                     /translation="MVENIAASVEPPRRFHFDWVSAVLFKPKQAFARITEQLNGVWLT
                     PLLIQTVTAVVLVLVLGYVSQTYGAGTTGEMPQIPEYYTPEQIAQIQQAMAGLQSPVF
                     IYFFPTLIALGKVWLGWLLVGGIIHLVSTMLGGRGNTTRAMNIVAWAGLPFAVRDIVR
                     VLGALIGRKALVSPGLAGFAPTNGSNLALFLAAFLALVDIYVIWHLILIVIGLRASNG
                     LPLGKAFLAATLTIVLLLVVQGLVAYFTSSLGGLTIIRPFF"
     gene            853..1623
                     /locus_tag="VN363_08155"
     CDS             853..1623
                     /locus_tag="VN363_08155"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559199.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84522.1"
                     /translation="MNIDLQASLNGSYVSVRSLRKTYVMGRIAVHALAGVDLEVQKNS
                     LTVIMGPSGSGKSTLLHLVGGLDRPTSGNIKVGEQALETLDENALAVFRRKSVGFIFQ
                     SFNLISSMTALENVEFPMRFSQISRRVRRSRAAELLDRVDLADRAHHKPTELSGGQQQ
                     RVAIARALVNDPPLILADEPTGNLDTASGASIMELLSTLHRSGRTVLVVTHDARMNQY
                     ATNTVSLLDGCIVEENALNRAFELPEAQGAENSPKTGN"
     gene            1625..>2827
                     /locus_tag="VN363_08160"
     CDS             1625..>2827
                     /locus_tag="VN363_08160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84523.1"
                     /translation="MIKNLTLHRIFAALLLSALILAAFPQTTLAAIGISGVQPRVVSN
                     TTSADLVITGSDFVDGAVVVLEGWGGLNTSFVSATLLTAGLPAGLPTGSYGITVINPD
                     SSSAYLANALQVVQASQTPEEPGGDVTPTPYTRPLVIVDSYNTSVSKITPGQEFKLVV
                     KLRNTGGREATNVVATFTPGSLVPRSTGGVAAIKELDPGETRKVEQPFTATYDILGAT
                     VAAQVMMLTYTDSAGTTYTETFNLSLPVATFSGVVGPTATPTPTATVPARPRPQLVIN
                     AYQTDIDTLTPGSRFSLDLTINNVGNADARRVTMILGGGTSGSTNPEGTPEPGGVSGS
                     GGDFGNFAPVAASNVQFLGDLSAGSTLPANAHLIVNATANPGAYPMKISFVYNAPDNI
                     SYTDDQVIT"
BASE COUNT          554 a          826 c          800 g          647 t
ORIGIN      
        1 agaatgggct tggcacagtg cccattcgcc aacctttgcg cggcatcgag aaaggacctc
       61 aagctcatgg ttgagaatat tgctgcctct gttgagcccc cacggcgttt ccattttgat
      121 tgggtttccg ctgtcttatt caaaccaaag caggcttttg cccggatcac cgagcagctg
      181 aatggcgtct ggctgacgcc gcttctaatc cagaccgtga ctgctgtcgt cctggttctg
      241 gtattgggct atgtctccca gacctatggg gcggggacga ccggggagat gccccaaatt
      301 cctgagtatt acacccccga acagattgcg cagatccaac aggcgatggc tggtctgcaa
      361 agcccggtgt ttatttattt tttccccacc ttgattgcac ttggcaaagt ctggctggga
      421 tggctgctgg tgggtgggat aatccacctg gtttctacca tgctgggcgg gcggggcaat
      481 accacccggg ccatgaatat cgttgcctgg gcggggctgc cttttgctgt gcgtgatatt
      541 gtccgcgtgc tgggggcgtt gatcggtcgc aaggccctgg tctcgcccgg gctggctggt
      601 tttgcgccta ccaatggcag caacctggcc ctgttcctgg cagcctttct ggcgctggtt
      661 gatatctatg tcatctggca tctgatcctg atcgtgattg gactgcgcgc cagcaacggc
      721 ctgccgctcg gtaaggcttt tctggcagcg accctcacga tcgtgctgct cctggtcgtc
      781 cagggcctgg tagcttactt cacttccagc ctgggtggac tgacaattat ccggccgttc
      841 ttctaaatct ttatgaatat cgatctgcaa gcctctctaa acggttctta cgtcagcgtc
      901 cgcagtctgc gaaagaccta tgtaatgggc cgcattgccg tacatgccct ggcgggtgtg
      961 gacctggaag ttcaaaagaa ttctttgacg gtcatcatgg gcccttcggg atcaggtaaa
     1021 tcgaccttgc tgcatctggt cggtggcttg gaccgcccca cgagtggcaa tataaaggtc
     1081 ggcgaacagg ctctggagac gctggacgag aatgccctgg ctgtcttccg gcgtaaatcg
     1141 gtcggtttta ttttccaatc cttcaatctg atctcgagta tgactgccct ggagaatgtt
     1201 gagttcccca tgcgtttttc ccagatctct cgccgggtga ggcgcagccg ggccgccgag
     1261 ctgctggatc gtgtggatct ggcagaccgg gcgcatcaca agcccaccga gctatctggc
     1321 gggcagcagc agcgggtagc gattgcccgg gcgctggtta atgatccgcc cctgatcctg
     1381 gctgacgagc ctaccgggaa cctggacact gccagcggcg ccagtatcat ggagctgctt
     1441 tccacactcc accgcagcgg ccggacggtt cttgtggtga cccatgatgc ccgtatgaac
     1501 cagtacgcga ccaacacagt cagcctcctg gatggttgca tcgttgaaga gaatgctctg
     1561 aaccgggctt ttgaactgcc tgaagcgcag ggcgcagaga attcaccaaa aacaggaaat
     1621 taaaatgatc aaaaacctta cccttcaccg gatatttgca gctttgcttt tatctgcgct
     1681 gatcctggcg gcattccccc aaaccaccct ggcagcgatc ggtatcagtg gggtccagcc
     1741 gagagttgtc tccaatacca cttcggctga cctggtgatt actggcagcg actttgtcga
     1801 tggggcggtg gttgtcctcg aaggctgggg aggcctgaat acgagctttg tcagcgctac
     1861 cttgctcacc gccgggctgc cggccgggct gcccaccggc agctatggga ttacggtgat
     1921 caatcccgat tccagctcgg cttatttagc caatgccctt caagtggttc aggccagtca
     1981 gacacccgag gaacccgggg gcgatgtaac tccgacaccc tacacccgcc cgctggtaat
     2041 cgtcgattcc tacaacacca gcgtcagcaa gattactccc gggcaggagt ttaaactggt
     2101 ggtcaagtta aggaataccg gaggcaggga agcgaccaat gtggtcgcca cctttacacc
     2161 ggggagcctg gtgcctcgca gcactggcgg ggtagcggcg attaaagaac ttgatccggg
     2221 tgaaacgcgt aaagtagagc agcctttcac tgccacctac gacatcctgg gcgcgaccgt
     2281 tgctgcccag gtaatgatgc tgacctatac cgacagcgcc gggacaacct atactgagac
     2341 atttaatctg agcctgccgg tcgccacctt cagcggcgtg gtgggtccta cagcgactcc
     2401 taccccgacc gccacggtgc cggcgcggcc tcgcccgcag ctggtgatta acgcctatca
     2461 aacggatatc gacaccctta cccccggatc gcgcttctcc ctcgacctga cgattaacaa
     2521 cgttggcaac gccgacgcca gacgggtgac gatgatcctg ggtggcggaa ccagcggtag
     2581 caccaaccct gaaggtaccc ccgagcctgg cggagtctcg ggttcgggtg gagatttcgg
     2641 gaatttcgcc ccggtggcag cctctaatgt ccagtttctg ggcgatcttt ctgccggaag
     2701 cacattacct gccaatgccc acctgatcgt caatgctacc gccaatccgg gcgcttaccc
     2761 gatgaaaatt tcgttcgtct ataacgcccc ggataatatc tcctacaccg atgatcaggt
     2821 gattacc
//
LOCUS       DATMXK010000441         4493 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_50530, whole genome shotgun sequence.
ACCESSION   DATMXK010000441 DATMXK010000000
VERSION     DATMXK010000441.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4493)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4493)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4493
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50530"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(185..409)
                     /locus_tag="VN363_08165"
     CDS             complement(185..409)
                     /locus_tag="VN363_08165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84524.1"
                     /translation="MVADPLERSLYVSVHYPEALFDDAEDILGAEAAGLVTIETPGVV
                     VLCPIVSEEAAEAPGNTQSSEDFPPFPDTF"
     gene            complement(440..1702)
                     /locus_tag="VN363_08170"
     CDS             complement(440..1702)
                     /locus_tag="VN363_08170"
                     /inference="COORDINATES: protein motif:HMM:NF024829.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWQ84525.1"
                     /translation="MTLVILGLKSLEPIPTNGFTLSASQTQSVQADPTAAALTIEGQL
                     VPRSQVSLSTEMDGQVAEIMIQEGDWVTPGTPLLRLDNREQLQANVAAAEYELIIAQQ
                     MLDGLYEEVQLDSALQEKALAETQKELARAEGNLKNLSTPATPAEIEQVYANLLLAEI
                     RLEMVEEDIRIWEKRQANKQNIYWTILKMDRGDFNDILEKLEWQRINAQRQVDISQEK
                     NNDLLYPDNEFELAQAEAQVNLLQAQLDDIEREISTLQNGPDPDAVADARVKLQAAEA
                     GLMATQSALDMAELVAPQTGRVAEITVEAGEWIQAGQPALILIDDSQWVIEIEELTEK
                     DVPQVEVGQAVSVQSKVLPELELSGSVESIRLLSREERGKVYYTVAILLDQTDPHLRW
                     GMTVDVIFQKAVGFCWRCISSSHLAVDD"
     gene            complement(1788..2972)
                     /locus_tag="VN363_08175"
     CDS             complement(1788..2972)
                     /locus_tag="VN363_08175"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWQ84526.1"
                     /translation="MKSKTIKAIGIFILGTLLLSACSGIGIIQTDTTPTLLPPVKVAS
                     AMIAEGRIVPNRHVKLAFKTNGQVAEILVEEGEIVEVGEVIARLEDSEQFASAAAAAE
                     VELLNAKQALDVLHENANVTEAQARQAVTDARDAVRDAERYLDNLRAGSRQTDIDEAE
                     ANVIILEDKLKKAQDDYAQYANKKKDSVVRATYQTKLADAQRKYDNAVSLLNNLTGNP
                     AELDIAIAEANLAVAQAQLAIDEKKYADLQNGVAPDDLAAAETRLRAAEMAQVAAQAA
                     LDNLSLVAPFSGTVADLQLEVGEQVALGKAVATLVDFSVWVVETTDLSEIEVPRVSVG
                     QSVTIGVDALPDLELIGTVESIGLVFTEKSGEITYTARIILADEDPRLRWGMTTVVTF
                     DE"
     gene            complement(3041..>4493)
                     /locus_tag="VN363_08180"
     CDS             complement(3041..>4493)
                     /locus_tag="VN363_08180"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84527.1"
                     /translation="GLLAFAIAVPFANLATDNLMKSLASKINFHYSGARFEPIALAVQ
                     AILALIVPQVAGFIPILQGTRISVREALSGTRQTDAPDKSWIDRRLTRIRRLSRPFTI
                     SLRNVFRNKGRLILTLITLSLGGAVFISVFNVRVSLSNYMEQLSKYFLADLNLTMTRN
                     YRVEEIEQTLGAIHEVASVEAWGLARSSVILEDGSLSEDLNLIAVPGDSTLIDPILLS
                     GRWLLPQDENAIVLNDQFLAKFPDLHIGDTLKLQVNDQETEWVVVGYFQLAGKIGGLA
                     AYVNLDYLVTLPGQIQPKHASSFRVVAHGSHDSADQKALAKEVEGMLAANSFQISAIS
                     TGNSMNESSSAGFAILTNLLLILAILTAMVGSIGLTGTMSMNVMDRTREIGVMRSIGA
                     SDPLLMRMVMMEGLVIGWISWLIGALLSFPISQLMSDMVTNALFGAPSTFGFTTTGFI
                     IWFAVVSILSIGASFLPARNAARLTIREVLAYE"
BASE COUNT          976 a         1266 c         1169 g         1082 t
ORIGIN      
        1 aaacggaatg gggtttggtg tgtatcgccc ataatatgca aaaactggcg ggctgatttc
       61 cgccagtttt tttatctttc accagaattg cccttcaagg gctgttttga ggttatctgg
      121 ctgttctttc tggacagcct cgcgcctgag gaatgatgac ccgggcgggt catcagccgt
      181 gaccctaaaa cgtatcgggg aagggtggga agtcctcgga tgattgggtg tttcccggcg
      241 cttccgcagc ttcttcggat acgatcgggc ataggacaac cacgcctggg gtttcaatcg
      301 taaccaggcc agccgcctca gcgcccagaa tgtcctcggc gtcatcgaag agtgcctctg
      361 ggtagtgcac ggaaacatac aatgatcgct cgagcggatc agcaaccaac gcaatctgcc
      421 tagagatgtg cacggctgat cagtcgtcga ccgccaagtg ggagctgctt atacatcgcc
      481 agcagaagcc gactgccttt tggaaaatca catcgactgt cattccccaa cgcagatgag
      541 ggtcggtctg gtcaagcaag atggcgacgg tgtagtagac tttgccacgc tcttctcgtg
      601 acaacaaccg gatcgattcg acagacccac ttagctccaa ctccggcagc actttggact
      661 gtacgctgac cgcctgccca acctcaacct ggggtacatc tttctcggtc agctcttcga
      721 tttcaatcac ccattgactg tcatcgatca ggatgagcgc cggctggcct gcctgaatcc
      781 attcaccggc ttcaacggtg atctcagcca ccctgccggt ctgtggagcg accagttctg
      841 ccatgtccag ggcgctttgg gtagccatca gcccggcctc ggcagcctgt aacttcaccc
      901 gggcgtcagc gacggcgtct gggtcaggcc cgttttgcaa tgtactgatc tctcgctcaa
      961 tgtcgtccaa ttgagcctgg agcaaattca cctgagcctc tgcctgggcc agttcgaact
     1021 cattgtcggg ataaagcagg tcattattct tttcctgtga aatgtcgacc tggcgctgag
     1081 cgttgatcct ctgccactcg agtttctcca gaatatcatt gaaatccccc cggtccatct
     1141 tcaggatcgt ccagtagata ttttgcttgt tggcctggcg tttttcccag atgcggatat
     1201 cctcttcaac catttccagc cggatctctg ccaggagcag gttggcgtat acttgctcga
     1261 tctcggctgg tgttgccgga gtactcaggt tcttcaggtt gccctccgcc cgtgcaagct
     1321 ctttttgtgt ttcggcaagg gctttctcct gcaatgccga atccaattgg acctcttcat
     1381 aaagtccgtc caacatctgt tgagcgatga taagttcata ctccgcagca gccacatttg
     1441 cttgtagttg ttcacggtta tccaatcgta atagaggcgt tccaggtgta acccaatcgc
     1501 cctcctggat catgatttcc gccacctgcc catccatttc cgtggacagg ctgacctgag
     1561 atcgggggac caattgccct tcgattgtca aagcagccgc tgttggatca gcctgcacag
     1621 actgggtttg cgatgccgag agcgtgaatc cattcgtcgg gatcggctcc aggcttttta
     1681 atcccagtat gacgagcgtc aacactattc cgagtagtaa gaggaagaaa attaatttcc
     1741 gtaaagacgg attcatattc ttatcccatg tccgattggc gatcgaatca ctcatcaaag
     1801 gttacgacgg tagtcatgcc ccaacgcagg cgcggatcct catcagcgag gatgatacgc
     1861 gcagtatagg tgatctcacc gcttttctct gtgaagacga gcccgatcga ctcgactgtg
     1921 ccgatcaatt ccaggtctgg cagggcatcc acaccaattg tgacactttg cccaacgctg
     1981 acccgcggca cctcaatctc gctcaaatcg gtagtttcaa cgacccaaac ggagaagtcg
     2041 accagagtgg cgactgcctt acccagggca acctgttcac cgacttcgag ctgcagatct
     2101 gctaccgtac cggagaaggg tgccaccagg ctcagattgt ccagcgcggc ctgggcggca
     2161 acctgtgcca tctcggccgc gcgcagccgg gtctcggctg cagccagatc atccggggcg
     2221 acaccgtttt gcaggtccgc atacttcttt tcgtcgatcg ccagttgcgc ctgagcaacg
     2281 gccaggttgg cttcggcgat tgcgatatcc aactcggcag gattccctgt cagattgttc
     2341 aacaggctta ctgcgttgtc atattttcgt tgggcatcag ccagcttggt ctggtaggtc
     2401 gcccgtacga cactgtcctt tttcttgttg gcatactgtg catagtcatc ctgggctttt
     2461 tttaacttat cctccaggat gatcacgttg gcttccgcct catcgatatc cgtctgccga
     2521 ctgccagcgc ggaggttgtc cagatagcgt tccgcgtccc gcacggcgtc gcgcgcgtca
     2581 gtgactgcct gcctggcctg tgcctcagtc acattagcgt tttcatggag cacatccagc
     2641 gcttgcttgg cattgagcag ttcaacctca gcggccgccg ctgctgaggc gaactgttcg
     2701 ctgtcctcca acctggcgat gacctccccg acctcgacga tttcgccctc ctcgaccaga
     2761 atctcagcga cctgtccgtt ggttttaaag gcgagtttga catgccggtt cggtacaata
     2821 cgtccctctg caatcatggc gctggctact tttaccggtg gcagcaaagt aggagtggtg
     2881 tctgtttgaa tgatgccgat cccactgcaa gcactaagca gcagcgtgcc caaaatgaat
     2941 attccgatcg ctttaatagt tttacttttc atagtttctt tctccctatt gatacacttt
     3001 tggttttgtc gattacccat agatggaagt tttcgataag ctattcatac gccagtacct
     3061 cacggatagt cagccgggct gcattgcgcg ccggtaggaa gcttgccccg atcgatagaa
     3121 tggaaacgac agcaaaccag atgataaagc cagttgttgt aaaaccaaag gtagacggcg
     3181 cgccaaagag cgcattggtc accatatcgg acatcaattg actgattggg aaggagagca
     3241 aagcgcctat cagccaactg atccagccga ttaccaaccc ttccatcata accattcgca
     3301 tcagcaatgg gtcggaagcg ccgatcgagc gcatgacgcc gatctcgcgc gtgcgatcca
     3361 ttacattcat gctcatcgtc ccggtcaagc cgatgctgcc taccatagca gtcaggatag
     3421 ccaggatgag caggagattg gtcaagatcg cgaacccggc ggatgaggat tcgttcatac
     3481 tgttgccggt ggatatggcg ctgatctgga aactgttagc agccaacata ccctcgactt
     3541 ccttcgccag cgctttctgg tcagcgctat catgactgcc gtgggcaacg acccggaatg
     3601 atgaggcgtg cttaggttgt atctgtccag gcagggtgac caggtagtca aggttgacgt
     3661 aggccgccaa tccgccgatc ttcccagcca gttggaagta acctaccacc acccattcgg
     3721 tctcctggtc attgacctgc aacttcaagg tatcaccgat atgcaagtcg gggaattttg
     3781 ccaggaactg atcgttcagc acgatggcgt tctcgtcctg aggaagcaac cagcgtcccg
     3841 aaagcaggat tggatcaatc agggtactgt cgcccggtac ggcgatcagg ttgaggtcct
     3901 cactcagcga gccatcctcc aagattactg aactgcgcgc taacccccag gcttcaaccg
     3961 aagcgacttc gtgaatggcg ccgagcgtct gttcgatctc ttccacacga taattacgcg
     4021 tcatagtcag gttgaggtcc gccagaaaat acttgctgag ttgctccatg taattcgata
     4081 gcgagacgcg cacattgaac accgaaatga acactgcgcc acccaggctc agggtgatca
     4141 gggttaggat cagccttccc ttattgcgga acacattgcg aagagagatg gtgaagggtc
     4201 gagaaagcct acggatgcgc gtcagtcgcc gatcgatcca gcttttatcg ggcgcgtcag
     4261 tctggcgggt gccgctcaag gcctcacgca ccgatatgcg cgtcccctgc aagatgggaa
     4321 taaaacctgc tacctgcgga acgattaaag ctaatattgc ctggacagcc agtgcaatcg
     4381 gttcgaaacg tgcgccacta tagtgaaaat tgatcttact ggctaaagat ttcatcaggt
     4441 tgtctgtcgc aagattagcg aaggggacgg cgatggcgaa agccagcaaa ccg
//
LOCUS       DATMXK010000442         2120 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_154891, whole genome shotgun sequence.
ACCESSION   DATMXK010000442 DATMXK010000000
VERSION     DATMXK010000442.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2120)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2120)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2120
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_154891"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..991
                     /locus_tag="VN363_08185"
     CDS             <1..991
                     /locus_tag="VN363_08185"
                     /inference="COORDINATES: protein motif:HMM:NF012784.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="S1 RNA-binding domain-containing protein"
                     /protein_id="HWQ84528.1"
                     /translation="KDTYRDIHGGPPPMDDSWWEAVMAEDESEAITHLQKSSEPVGYY
                     NKPPVQRPCNDNAAMDWEKAQQLYGMDQTIDLCVTGYNRGGLLVEDDTLQGFVPISHL
                     VEMQEDIAESEQEAWLARYVGQTLTLKVIECDQSRGRVVFSERAALSKPGSRNILLGN
                     LSPGMCIPGTVTNITDFGVFVDLGGVEGLIHVSEISWGRVRHPRDVVKLGCTVNVYVI
                     QVNRELARVALSLKRLYPNPWETAEERYHPGQLIGATVTSIVPFGAFARLEEGLDGLI
                     HVSEICPGENVGSLADLIQEGQQVYVRVLHVDGSRQRLGLSLSLEDNPDLALP"
     gene            1148..1738
                     /locus_tag="VN363_08190"
     CDS             1148..1738
                     /locus_tag="VN363_08190"
                     /inference="COORDINATES: protein motif:HMM:TIGR00426.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-hairpin-helix domain-containing protein"
                     /protein_id="HWQ84529.1"
                     /translation="MKTWQATLFAVLCTLLVTGIVYLVSQPERGAPIQLLPAPTPSPL
                     VIQISGAVHQPGMYPLPAGSRLNDAIQAAGGLLPEADNSQLNLASPLVDGSLVVIPQK
                     SDPDASAITAPGFLPTSQPRSGAIIVQGLLDINRATAGEFESLPEIGPVIAQRIVDYR
                     SANGPFTTLEAIQNVSGIGPAKYEKIKPLITLDPAP"
     gene            1749..>2120
                     /locus_tag="VN363_08195"
     CDS             1749..>2120
                     /locus_tag="VN363_08195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84530.1"
                     /translation="MKAAFHREITTSALHSRVSPHALEAILRANLSQDGWVGLLFHPE
                     FHFDGSRFDAGEAYLSAQHDLARSFLIKGDPLPAWQAFGRLSHASQDFYAHSNYVQLF
                     LKAYPHSLPAQIDPLLPVILND"
BASE COUNT          458 a          601 c          582 g          479 t
ORIGIN      
        1 aaaagatact taccgcgata tacatggggg ccctccgccc atggacgatt cctggtggga
       61 ggccgttatg gctgaggatg aatcggaagc cataacccac ttacaaaaaa gcagcgagcc
      121 ggtggggtac tataacaaac ctcctgttca acgcccatgt aacgacaatg cagcgatgga
      181 ttgggaaaag gcccaacaac tttacggcat ggaccagacc attgatctct gcgttaccgg
      241 ttataaccgg gggggactgc tcgtcgaaga cgataccctg cagggatttg tcccaatttc
      301 ccacctcgtg gaaatgcaag aggatatcgc tgagagtgag caagaagcct ggctggccag
      361 atatgtgggg caaaccctga cgcttaaagt gattgaatgc gatcaaagcc gggggagggt
      421 cgttttctca gagcgagctg ctctgtcaaa accgggcagc cgcaacatcc tgctcggcaa
      481 tctcagcccc gggatgtgca ttccagggac ggtcaccaac ataaccgact tcggtgtatt
      541 tgtggatctt ggtggggttg aaggcttgat ccatgtatcc gagatctcgt ggggacgggt
      601 acgccacccc cgcgatgtgg tcaagctggg ctgcacggtc aatgtgtacg taattcaggt
      661 taaccgcgag ctcgcccggg tcgccctgag tctgaagcgc ctgtatccca atccctggga
      721 gactgcggaa gagcgctacc acccgggcca gttaatcggc gccacggtta ccagtattgt
      781 tccgtttggc gctttcgctc gtctggaaga ggggctggat ggcctgatcc atgtttcaga
      841 aatttgtcct ggcgaaaacg taggttcact ggccgattta atccaggagg gccagcaagt
      901 atacgtgcgt gtgctgcatg tagatggttc acgccagcgt ttagggctca gcctgagcct
      961 ggaggataat ccggatctgg cgttacctta gcgccctggt catatcaata ccctcctcac
     1021 cagtatcttg agtttggata tatcgctgcc gggaagggcc gccttaaccg gcagccatcg
     1081 ggtggatgac cacacacgac caaactctat ggtgcaggtt tttgattcaa cccggtgggc
     1141 atgacggatg aaaacctggc aggcaacttt attcgcagtc ttatgcacac ttttagtgac
     1201 tggcattgtc tatctggtta gccagcccga gcgcggcgct cccatccagc tccttcctgc
     1261 tccgaccccc tcgccgctgg tcattcaaat ctcaggagct gtccaccaac ccggcatgta
     1321 cccgcttcct gctggcagtc gcctgaacga tgcaatccag gctgcaggag gcctcctgcc
     1381 agaagctgac aacagccagc tcaatctggc gagtcccctg gttgacggca gtctggtggt
     1441 cattccccag aaatctgatc cagacgcttc cgcgatcaca gcgcccgggt ttctgcctac
     1501 ctcccagcct cgcagtggag cgatcatagt gcaggggctg ctggatatca atcgggcaac
     1561 tgcaggtgaa tttgagagcc tgcctgagat tgggccggta atcgcccagc gcatcgtgga
     1621 ttaccggtcg gccaatggtc ctttcacgac cctggaagct atccagaatg tctcagggat
     1681 cggtccggcc aaatacgaaa aaattaaacc cttgatcacc cttgacccag caccttgaat
     1741 aaactgccat gaaagccgcc ttccaccgcg agatcacaac ctctgcgctg cactcccggg
     1801 ttagcccgca cgctctggaa gcgatcctgc gcgcgaattt aagccaggat gggtgggtgg
     1861 ggctgttgtt ccatccggaa tttcattttg atgggagccg gttcgacgca ggagaagctt
     1921 acctgtctgc tcagcatgac ctggcgcgtt cgtttttgat aaagggagat cctcttccag
     1981 cctggcaggc tttcgggcgg ctctctcatg ccagccagga tttttatgcc cattccaatt
     2041 atgtccagct ttttctgaaa gcctacccgc acagcttgcc tgcccagatc gatcccctcc
     2101 tgccggtgat ccttaatgac
//
LOCUS       DATMXK010000443         2395 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_107514, whole genome shotgun sequence.
ACCESSION   DATMXK010000443 DATMXK010000000
VERSION     DATMXK010000443.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2395)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2395)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2395
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107514"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..682
                     /gene="pstA"
                     /locus_tag="VN363_08200"
     CDS             <1..682
                     /gene="pstA"
                     /locus_tag="VN363_08200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558696.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWQ84531.1"
                     /translation="GGVFPAILGTVYLTLLTTLLSVPLGIAAAIYLAEYAPDNLMTRI
                     IRIAIINLAGIPSIVYGLFGLGIFVIFFKFGTSILAASFTLAIMTLPTIISTAEEALR
                     AVPQSFRVVSVSLGATQWQTIRHVVLGQSLPGILTGVILGLERAAGETAPILLTGAAF
                     FLPALPTSPMDATMALPYHLYVISTQVPGMPVEIQFGTAVVLLILVLGLNILATIIRS
                     RARARRQW"
     gene            698..1468
                     /locus_tag="VN363_08205"
     CDS             698..1468
                     /locus_tag="VN363_08205"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558697.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006817 - phosphate ion transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein"
                     /protein_id="HWQ84532.1"
                     /translation="MNKFEIKDFSLRYSDGNESLRQINLAIPAHSITVLFGPAGGGKS
                     TLLRVLNRLNDLADVVSLSGQVTLFGEDGSPLDILEPKLDVTALRRRVAMVFARPIVL
                     PMSIRENLTYGLKIAGVRDPVRLAEAVESSLQKAALWEEVRDRLDQPAVALSGGQQQR
                     LCIARSLALEPEVILLDEPTSGLDPISTGKVEDSLHELKARYTVVLVPHSVQQAARMA
                     EQAAFFLQGELIEYGPGKTIFLTPLNRKTQDYVEGRFG"
     gene            1607..2290
                     /gene="phoU"
                     /locus_tag="VN363_08210"
     CDS             1607..2290
                     /gene="phoU"
                     /locus_tag="VN363_08210"
                     /inference="COORDINATES: protein motif:HMM:TIGR02135.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0051049 - regulation of transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate signaling complex protein PhoU"
                     /protein_id="HWQ84533.1"
                     /translation="MTRANLDRKIKNMLNEIMIIDSMVESATLGAIQALKNRDLDAAR
                     TIYQADAPINARRFALEKECMVTIATEQPIMAGDLRVVASILEVVNELERMGDYAKGI
                     ARVCLRIGDQEHIKPVIDLPLMAEVAVDMLHRAVAAFVAQDSEQARLIPAEDTRVDDY
                     YQRIYADLIAIITQEPAAAYQANLLMWAAHNLERMADRVTNICERTIYITTGEMIEID
                     ATDDEDLSL"
BASE COUNT          458 a          829 c          617 g          491 t
ORIGIN      
        1 cgggggtgtc ttccccgcca tcctgggtac cgtttacctg acattattga ccaccctctt
       61 atccgtcccc ctgggcatcg cggctgccat ctacctggcc gaatatgccc ccgataatct
      121 gatgacccgc atcatccgca tcgccatcat caacctggcg ggcatccctt ccattgtcta
      181 cggtctcttt gggctgggta tcttcgtgat cttcttcaaa ttcggcacca gcatcctggc
      241 cgcttccttc accctggcaa tcatgaccct gccgacgatc atcagcaccg ccgaagaagc
      301 tctgcgcgcc gtccctcaat ctttccgggt ggtgtccgtc tcgctgggcg ccacccagtg
      361 gcagaccatc cgccacgtgg tgctgggcca atccctgccc ggcatcctca ccggggtcat
      421 cctcggtctg gaacgggctg ccggtgaaac tgcccccatc ctgctgacgg gcgctgcctt
      481 cttcctgccg gccctgccca cttcccccat ggacgccacc atggctctgc cttaccacct
      541 gtatgtcatc tccacccagg tccccgggat gccggtcgag atccagtttg gcaccgccgt
      601 tgtcctgctc atcctggtcc tgggcctgaa catcctggcg accattatcc gctcccgggc
      661 gcgggcccgc cgccagtggt agccgccggt acagccaatg aacaagtttg agattaagga
      721 tttctccctg cgctactcgg atggcaacga atcgctgcgc cagatcaacc tggcgatccc
      781 cgcccattcc atcaccgtcc tgttcggccc tgccggcggc ggcaaatcca ccctgctgcg
      841 cgtccttaac cgcctgaatg atctggcgga tgtggtcagt ctttccgggc aggtgaccct
      901 gttcggtgaa gatggcagcc ctctcgacat actcgaaccc aaactcgacg tcaccgcctt
      961 acgccggcgg gtggcgatgg tttttgcccg ccccatcgtc ctgcccatga gcatccgcga
     1021 gaatctgacc tacgggttga agatcgccgg cgtgcgcgac ccggttcgcc tggcagaagc
     1081 cgtcgaatcc agcctgcaga aagccgccct gtgggaagaa gtgcgcgatc gcctggacca
     1141 gcccgcggtg gccctttccg gcggccagca gcagcgcctg tgcattgccc gcagcctggc
     1201 cctcgagccc gaagtgatcc tcctggatga gccgacctcc ggcctggatc ccatctccac
     1261 cggcaaagtg gaagattcac tccatgagct gaaagcccgc tacaccgttg tgctcgtccc
     1321 ccactccgtc cagcaggccg cccgcatggc cgaacaggcc gccttcttcc tgcagggcga
     1381 gctgatcgaa tatggtccgg gaaaaaccat cttccttacc ccgctcaacc gcaaaaccca
     1441 ggattatgtg gagggtcgtt ttggctaacc ctcaatctcc tccctccgta ggggcgaagc
     1501 ggcgcctacc agggtttccc tcgccacatg ctgcagcttt gccctgtcag ctgatcaata
     1561 ttgagaatca atttacgtta caatcattcc aaggaagggt attcccatga cccgtgcaaa
     1621 tctggatcgc aaaattaaaa atatgctcaa tgagatcatg atcatcgaca gcatggtcga
     1681 gagcgccacc ctgggcgcga tccaggcctt gaagaaccgc gatttagacg ccgcccgcac
     1741 catctaccag gccgatgcgc cgatcaacgc tcgccgcttc gccctcgaaa aggagtgtat
     1801 ggtcaccatc gcgaccgagc agcccatcat ggctggtgat ttgcgcgtgg ttgcctccat
     1861 ccttgaagtg gtcaacgagc tggagcggat gggcgactac gccaaaggaa tcgcccgggt
     1921 ctgtctgcgc atcggcgacc aggagcatat taagcccgtt atcgacctgc cgctcatggc
     1981 cgaggtcgcc gtcgatatgc tccaccgcgc cgtggcggcc tttgtcgccc aggacagcga
     2041 gcaggcgcgc ctgatcccgg ccgaagacac ccgggttgat gactattatc aaagaatcta
     2101 cgccgacctg atcgcaatca tcacccagga gcctgccgca gcctaccagg ccaatctgct
     2161 catgtgggct gctcacaacc tcgagcgcat ggctgaccgg gtcaccaaca tctgcgagcg
     2221 tactatttac atcaccaccg gtgagatgat cgaaatcgac gccaccgacg acgaagacct
     2281 cagcctatga tggcactgct cgcgccgcgc agcagccagt ctcacctttc gcaaacatcc
     2341 ccaacctcca aaaaagtgga ttagtcccac cttaattttt caatttcata cttga
//
LOCUS       DATMXK010000444         4758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_44106, whole genome shotgun sequence.
ACCESSION   DATMXK010000444 DATMXK010000000
VERSION     DATMXK010000444.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4758
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44106"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(278..1453)
                     /locus_tag="VN363_08215"
     CDS             complement(278..1453)
                     /locus_tag="VN363_08215"
                     /EC_number="2.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF015679.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042834 - peptidoglycan binding [Evidence
                     IEA];
                     GO_function: GO:0071972 - peptidoglycan L,D-transpeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L,D-transpeptidase"
                     /protein_id="HWQ84534.1"
                     /translation="MTVKHHKDSLTRRDFLKLAALGIGSLAFRSRSRSLYQPEFPQAE
                     LLGRVNAGKVELKARPDADSQTTGALYEDAVVPWLREVAGKNIYRTNQRWVETPEGFI
                     WSPYLQPVRNAPNPPLQSLPVTSLGAGMWVEVSIPYVDLALDNPPARSPGYQDRIKLG
                     LPLRLYYSQIVWVDQVRMDADGQAWYRVNEKYGSYGDILWGEGQAFRPLNSEEMAPIS
                     PDVEDKRVVVDITYQTLSCYEGKNEVYFTRISSGALYNYAGEKIDVWATPIGKFPIWR
                     KLVSLHMSGGTTGGGWDLPGIGWVSLFVGSGVAVHSTFWHNNFGEPMSRGCVNARPED
                     AKWIFRWTQPLVPYDPGDITVGLPGGTKVEMKHDDIFKASYKNNQVLEYRRDINGRV"
     gene            complement(1608..2114)
                     /locus_tag="VN363_08220"
     CDS             complement(1608..2114)
                     /locus_tag="VN363_08220"
                     /inference="COORDINATES: protein motif:HMM:NF012575.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquinol-cytochrome c reductase iron-sulfur
                     subunit"
                     /protein_id="HWQ84535.1"
                     /translation="MNRNSTTHDLKRRDFVKIVMSFLGTVMAGIIGIPGIGYLVSPAT
                     RKAESNAWIALGPLENYPTGVPTPFNFTRSTINGWEKTVNSYGVYVIRQDESQVRVFS
                     NICTHLSCRVSWHTDIQHYVSPCHDGHFDIVGTVLSGPPPRPLDEFETKIEASNLYIL
                     FPPYRRTG"
     gene            complement(2150..2683)
                     /locus_tag="VN363_08225"
     CDS             complement(2150..2683)
                     /locus_tag="VN363_08225"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84536.1"
                     /translation="MKYFTFLFLLVIGLILAGCSAAVPSSTIQPSVELSAITPSLSPA
                     TPTQAATDQPVEPSGAQVIQLAELTDSQGAVTVIVKSLDLNRSQETLNFEMSLDTHSI
                     DLSMDLTALATLTTDTGLSVQAAKWDAAMGGHHVSGTLSFPSTLEGKAILDGASTLTL
                     IIKDVDASERVFAWDLE"
     gene            complement(2680..3183)
                     /locus_tag="VN363_08230"
     CDS             complement(2680..3183)
                     /locus_tag="VN363_08230"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84537.1"
                     /translation="MSTTKRILLPLAVSLAGALLLTGLYFGIVSWAESPQHAAELFWE
                     DRWIVFPIILGFGVQAGLYTILKMRLFVPVATTGPSGPIMGAGGMTSTIAMAACCAHH
                     VADVLPILGLTAAATFLAQYRIAFMLVGLGTTLLGIMIMTLILIKERRKAIQMISAVA
                     SPILETL"
     gene            3450..3764
                     /locus_tag="VN363_08235"
     CDS             3450..3764
                     /locus_tag="VN363_08235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84538.1"
                     /translation="MSQNRTKKYPKKRPRQNQSWLPILVAFGGLLLVILAFLALRDKP
                     TPRADIEVTGAPSLKVDQEKIDLGDMKFNQPAQVSFQLTNVGDQTLRFTKDPFIEVVE
                     GC"
     gene            4114..4500
                     /locus_tag="VN363_08240"
     CDS             4114..4500
                     /locus_tag="VN363_08240"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84539.1"
                     /translation="MMSDSFPFLSLEQPCDEAITWVSRQVCGVGLQVMRTFDLQVARH
                     EPTDCPCPHHGTAQCDCQMVVLLVYGEDYQPISLVVHCHDGRTWFSMVDTPQQRANPR
                     LERIIRQALEVEIISPVRQVDWSHAS"
BASE COUNT         1104 a         1248 c         1240 g         1166 t
ORIGIN      
        1 ggacatggag aaaggaagac acggagatcg ggagaccacc tcaatgcctc cgcctctccg
       61 tgtcctcttc cctgcgtctc cttttccctg ggtcctcttc accgtgtcac tatgtcttct
      121 ctcattgttt caccattcac aaagcaggca cattattttc tttcaggcct cgttttcgat
      181 gaggcataag ggaagttctc cgctggaggc aaaactcccc gctccccgag gatctatagg
      241 ggtcgatctg ctgctccagc gcatcggcat agccagatta cacgcgacca ttaatatccc
      301 ggcgatactc aagaacctga ttatttttgt aactggcttt gaagatgtca tcatgtttca
      361 tttctacctt agttccaccc ggtaggccaa ccgttatatc accgggatcg tatggaacca
      421 aaggctgtgt ccagcggaag atccatttgg catcttcggg ccgggcgttt acacagcccc
      481 gcgacatcgg ttccccgaaa ttgttgtgcc agaaggtaga gtgcaccgcc acgccgctgc
      541 ccacgaagag cgagacccat ccgatcccgg gtagatccca accgccgccc gtcgtcccgc
      601 cgctcatgtg cagcgatacc agcttgcgcc agatggggaa tttcccgatt ggcgtggccc
      661 atacgtcaat cttttcccct gcataattat ataaagcccc ggaagagatg cgggtgaaat
      721 agacctcgtt tttgccctca taacaagaga gtgtttggta agtgatatcc actaccacgc
      781 gcttgtcttc aacgtccggg ctgattggag ccatctcttc ggaattgagc gggcgaaagg
      841 cttgcccttc accccacagg atgtccccgt aggacccata tttttcgttc acccggtacc
      901 aggcttgccc gtcggcgtcc atccgcacct ggtctaccca cacgatctgg ctgtaataca
      961 ggcgcagagg caaccctaat ttgatgcggt cctggtatcc aggggagcgc gctggtggat
     1021 tatccaaggc tagatctaca taagggatac taacttccac ccacatcccc gcccccaggc
     1081 tggtaaccgg caggctttgg agtggcgggt tgggcgcatt gcgcacaggc tgaaggtaag
     1141 gagaccagat aaatccttct ggagtttcta cccagcgctg gttggtgcgg tatatgtttt
     1201 tcccagctac ctcgcgcagc caggggacga ccgcgtcttc gtaaagcgcg ccagtcgtct
     1261 ggctgtccgc atccgggcgg gccttgagtt ccactttgcc ggcgttgact cttcccagaa
     1321 gctcggcttg agggaactct ggctggtata aagaccgact tcttgaacga aacgccaggc
     1381 tgcctatacc caaggcagcc aatttgagaa agtcacgccg cgtgagagaa tctttgtggt
     1441 gttttactgt cattgagtga tttcgtcgct ctaatcagct gcttggattt gaccatgagc
     1501 cttttcgtgt tcgtgaccca taaattgcat cctgagccgg tgtgaaagcg ggcagcctgc
     1561 tatcataatg accatatgct ttctgttcat tttgagtatc gccttttcta cccggttcgc
     1621 ctataaggcg ggaaaaggat atacaaattg cttgcctcaa ttttggtttc gaattcatcc
     1681 agcggtcgcg gcggcgggcc gctgagcacc gtgccaacaa tatcgaagtg tccatcgtga
     1741 caggggctta cataatgctg tatatcggtg tgccatgaga ctcggcagct cagatgagtg
     1801 caaatgttgg aaaatactct tacctggctt tcgtcctggc gaatgacata cacgccataa
     1861 ctgtttaccg tcttctccca cccattgatg gtggagcgag tgaagttgaa gggagtgggg
     1921 acaccggttg gatagttttc taagggtcca agtgcaatcc aggcattgct ctccgcttta
     1981 cgagtcgcgg gtgataccag gtatccaatg cctggaatac cgataatgcc tgccatcacg
     2041 gttccgagga aagacataac gattttgacg aaatcccggc gctttaaatc gtgagtggtc
     2101 gaatttcggt tcatgattac ttcctcttgg ggttcattcc taacagtgat tattccaaat
     2161 cccaggcaaa gacgcgctca gaagcatcga catctttgat aatgagggtc agtgttgaag
     2221 cgccatccag aattgctttg ccttccaatg tagatggaaa agagagcgtc ccggagacgt
     2281 ggtgcccgcc catcgccgca tcccatttcg ccgcctgaac actcagaccg gtgtcggtcg
     2341 taagcgtcgc cagtgccgtc agatccatgc tcaggtcgat agagtgtgta tccaggctca
     2401 tctcaaagtt tagtgtttct tgtgatctgt ttagatcaag cgacttaacg ataacagtta
     2461 ctgcgccctg gctgtcggtg agctcggcga gttgtatcac ttgagcgcca gacggttcga
     2521 cgggttgatc tgttgcagct tgtgtgggtg tggcaggtga tagggatggt gttatcgccg
     2581 atagttcgac agatggttgg attgttgaag acggtacagc agcactgcag cctgccagga
     2641 tcagcccaat cacgagcagg aacagaaaag tgaaatattt catagggtct ccagaatagg
     2701 ggaagcgaca gcggatatca tttgaatggc cttgcgtcgc tctttaatga gaattaaagt
     2761 catgatcatt atcccgagta gcgtcgtacc taatccgacc agcataaagg cgatgcgata
     2821 ctgcgccagg aaagttgctg ctgcggtcag tcccaggata ggcagtacat cggcgacgtg
     2881 gtgagcgcag caggctgcca ttgcaatggt ggaagtcata ccgcctgccc ccattatcgg
     2941 tccactggga ccggttgtcg ctacaggtac gaacaatcgc attttaagga tggtgtacag
     3001 cccggcctgc acaccgaagc ccaggatgat aggaaaaacg atccagcggt cctcccagaa
     3061 taattcagca gcatgttgag ggctctctgc ccaagaaacg atgccaaagt agagaccggt
     3121 taggagcaga gccccggcca ggctaactgc caggggtaaa aggatacgtt tagtggtaga
     3181 catctgagga cagctccatt tgacagaatt ctcttcagtc ttttcatcat aaccaggccg
     3241 gcatcgattg tcatccgcca cgtagctctt tcaataatga gcagttttgc ctacaggtaa
     3301 gccattatgc ctataggcga atgtataaga ctgcgttata aatttcagat tacttctctt
     3361 ttgctctctg tgaattattt gcgccagaag gggtggtggc gcttctcgcc tatttacagg
     3421 tatttcgatt ttgaactgga ggatcaacca tgagccaaaa cagaaccaaa aaatatccca
     3481 aaaaacggcc aaggcagaac caaagttggc tgccaattct ggttgccttt ggcggtttgc
     3541 tcctggtgat tctggctttc ttggcgctgc gcgacaaacc cactcccaga gccgacatcg
     3601 aagtcaccgg tgcacccagc ctgaaggtag atcaagagaa aattgacctg ggtgacatga
     3661 agttcaacca acccgctcag gtctcgttcc agctcaccaa cgtgggtgat caaacactgc
     3721 gatttaccaa ggaccctttc atcgaagtag tcgagggatg ttgaccgccc ccattagcca
     3781 tcggttcgat ggcgctaaac ccgggagaaa gcaccacgat ctcaatgcaa ttcacgatgc
     3841 atgaaggtat ggatggcatg cataatttca gcgtccatct gccgagtaat gatccccagc
     3901 aacctgatca cactgtgacg gtattatcca actgggtccc ttgaacccga cattctgaac
     3961 tagagttccc atcccccgtt gttgtcaacg acttagcggg ggatggttat ttaaatgcgc
     4021 aattgtgcct atgcgggaat gcgatagatg gcgcttggtt taaaacaagt gagtttgtat
     4081 actggggcta atgaagcaaa agaacggagt tggatgatga gtgactcttt cccgttcctg
     4141 tccctggaac aaccttgtga cgaagccatt acctgggttt cccgccaggt ctgtggtgtc
     4201 ggcttgcagg tcatgcgcac atttgacctg caggtagccc gccatgaacc tacggattgt
     4261 ccctgccctc accacggcac cgcccagtgt gattgccaga tggtcgttct ccttgtttat
     4321 ggagaagatt accagccaat tagcctggtc gtccactgcc acgatggcag aacctggttc
     4381 tcaatggtgg acactccgca acagcgcgcg aaccctcgct tagagcgaat catacggcaa
     4441 gcactggaag ttgagatcat ctcacccgtg agacaggtag attggtctca tgcaagctga
     4501 tgaacaagct gaggtcatcc agcgattacg gtctgctgcg ggccatctcg aagcaatcat
     4561 caacatgttg gaggcaggtg agtcttgtga gccgctcctt caccagttag gcgcggtgcg
     4621 tgctgcactg cgagccgctg ggggtaggtt gctcgcctgt cagttgagac aaagccagga
     4681 cgttatccgg catagtccct cagcagaaga ccgggttgca gaaacacacc ggttattcat
     4741 actctatcac tttttgac
//
LOCUS       DATMXK010000445         4681 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_64359, whole genome shotgun sequence.
ACCESSION   DATMXK010000445 DATMXK010000000
VERSION     DATMXK010000445.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4681)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4681)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4681
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64359"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..197)
                     /locus_tag="VN363_08245"
     CDS             complement(<1..197)
                     /locus_tag="VN363_08245"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84540.1"
                     /translation="MEKNRSHNRLIFPSLILVMALVCTALILMHLSSNVNAVVVFPWE
                     GPSPTPTLWWRTPQASTPTPPT"
     gene            complement(167..1375)
                     /locus_tag="VN363_08250"
     CDS             complement(167..1375)
                     /locus_tag="VN363_08250"
                     /inference="COORDINATES: protein motif:HMM:NF016121.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWQ84541.1"
                     /translation="MTAAPKPSPRVRQRNKEFIDRLLGVVAIVTILAAYIFGLTRAET
                     DVIASFQQLVPSAQRFEPVSETVYAAYTGVQLVAYITLGEATGYGGDLEMAVAVDPQG
                     KLVNLAILDDQETSDYNKRVAGAGWPGVLAGKSCQDPFQLGQDVDGITGATYTASAIA
                     EAVRRGCRQMAALGVGDPSAAETRARIQFGVPEVTLILLFAVGFFAHRQKFKYTRQAR
                     WVSMIAGMLILGFWFNRPLSIININQLLLGTWPSWQNNLYWYLLLGGIFLVTTADNKN
                     PYCEWFCPFGATQECLGAVGGAKVRSPGKFKPYLVWLHRGLAWLAIVLALIFRNPTIS
                     SYEVFSGLFDRIASTPIFIVLGIVLVASLFIKRPWCTYLCPLQPVVEFIQMIRKWVIE
                     LWKKTVRTTA"
     gene            complement(1521..2144)
                     /locus_tag="VN363_08255"
     CDS             complement(1521..2144)
                     /locus_tag="VN363_08255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84542.1"
                     /translation="MNNTESVSGPASLTATEQASSVRSWIFRLLTLAGAAGMVYSWFQ
                     SWWSAYIETLDEIGVAIYPYKMIISGTLRDYPQWLVGAEMPVWFFPLMWVYLAVCVWL
                     LLYSLFTDSQDKVSLGKLKLSVPQILIGLAGLAYVVFVVVFPIVVSIRSADFYGVVLQ
                     GNVFISMNEHTESYVITALQPGYWIAVGTAVYLVSLAFLRKLIVGKA"
     gene            complement(2315..3127)
                     /locus_tag="VN363_08260"
     CDS             complement(2315..3127)
                     /locus_tag="VN363_08260"
                     /inference="COORDINATES: protein motif:HMM:NF024644.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ84543.1"
                     /translation="MKAFLFDAAKCNGCRSCQVACKDEHAGNDWMPYAKPMAETGQFW
                     YKQNEYIRGTLPKVKVSYVPTLCMHCDSGPCVTACVHGAISKRDDGLVLIDPAKCNGC
                     QLCVDSCPYGAISYNRALRIAQKCTGCAHLLDRGWPISEPRCTDACVTGALTFGEEAD
                     LAALISQAEVIEPKFGDNPKPRVFYLNLPKRFIAGTVYDPATEEVVIGANCTLAGDST
                     ATVTTDEFGDFWFEGLEVGSYAVTIEAAGLPTKAIDAISTEEDVNLGDIALA"
     gene            complement(3127..>4681)
                     /locus_tag="VN363_08265"
     CDS             complement(3127..>4681)
                     /locus_tag="VN363_08265"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0043546 - molybdopterin cofactor binding
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="molybdopterin dinucleotide binding
                     domain-containing protein"
                     /protein_id="HWQ84544.1"
                     /translation="YNTPTSIGYGRSGGGAVGRTIYADNPQRIQLYLQCMQGLGGPGK
                     HTLHQLQSAIGGAAQSPSAGGADPDTIWSTAMEGDVVMPEERQAFPRGYFAQAVLNPP
                     VEFYPVGKQFQKFTYPMPGKSEVHMIWGTSASYTGSHQWGFGVQKAMQSEKLECIVHQ
                     TMWMEDGLVFSDIILPIATAPEAPDINTTRDIFNSITLRTEPVMAPIGEARTDLAAVL
                     EVAKALGWYDKLTGGKPYDEMMQERLKAAYAASEITDLVSWEKLVEKGYFPQTPDPKW
                     YDREPAYKKFYDDPVANPLKTPTGLLEIESSLLKENFPDDKERPPVAHYVIGGPEADG
                     WTHDEDLTSERAKTYPLVIVSDTSTWKHHSMFSDVPWTREIEKITAWDGYAYAPVWIS
                     PKDADERGIQDGDIVKVFNERGAVLGGAVISQRIIPGALRFEKAGGGHHIIAGELHRG
                     GNPNCINPKSSFSRNVYGLACTHFLVQIEKVTGAQMQEWRESYPEAFARDYDPAYGPF
                     FTGWIEGAA"
BASE COUNT          987 a         1316 c         1422 g          956 t
ORIGIN      
        1 gtggggggag tgggcgtgct ggcctgcggc gtgcgccacc agagcgtggg agtcgggctt
       61 ggtccctccc aggggaagac aaccacagca ttcacattag acgatagatg catcaggatc
      121 agcgcggtgc agaccagcgc catcaccagg ataagcgagg ggaatatcag gcggttgtgc
      181 gaacggtttt tttccatagc tcgataaccc acttccgaat catctggatg aactcaacca
      241 ccggctgcag cgggcagaga taggtgcacc aggggcgttt gataaagaga gacgccacca
      301 ggacgatccc gagcacgatg aaaatcggcg tcgaagcgat ccgatcgaac aggccgctga
      361 atacttcgta gctggaaatg gtcgggttgc ggaagattag cgccaggacg atcgccagcc
      421 aggccaggcc gcggtggagc cacacgagat agggtttgaa cttccccgga gagcgcactt
      481 ttgccccccc gacggccccc aggcactcct gggtggctcc gaagggacag aaccattcgc
      541 agtaagggtt tttgttatcc gcagtagtga ccagaaagat gcccccgagg agtaaatacc
      601 agtacaggtt gttctgccag gagggccatg tgcccaacag cagctggttg atattgatga
      661 tcgacagcgg gcggttgaac cagaagccca gaatcagcat cccggcgatc atcgagaccc
      721 agcgggcctg gcgggtgtac ttgaacttct ggcgatgggc gaagaaaccg accgcaaaga
      781 gcaggatcag ggtgacttcg ggcaccccaa actggatccg cgctctggtt tctgcggcag
      841 acggatcgcc cacccccagc gcagccatct ggcggcagcc gcggcgaacc gcttcggcga
      901 tagcggatgc ggtatacgtg gcgccggtga tgccgtccac atcttggccg agctggaaag
      961 gatcctggca ggattttccc gccagcacac ccggccagcc ggctcccgcc acccgcttgt
     1021 tataatcgct ggtctcctga tcgtcgagga tggccagatt gaccagctta ccctgcggat
     1081 cgacagctac agccatctca aggtccccac cgtagccagt cgcttcgccg agggtgatat
     1141 aagcgaccag ctggactccg gtgtaagcag catagacggt ttcggaaact ggctcgaagc
     1201 gctgggcgga aggcacaagc tgctggaagc tggctattac atcggtttcg gcgcgggtaa
     1261 gcccgaaaat gtaagcggcc aggatggtca caatggcgac gacgcccaga aggcggtcga
     1321 tgaattcttt attgcgttgg cgaacccgcg gggatggttt cggggcggct gtcatcggta
     1381 aatcgctggc atggcacaga gttggattga attggcaccc ggattaacca ggatgatgcg
     1441 tggtaaaggg gacagggaaa actctccctg tccccttttt agcctggcgc cggcagctgc
     1501 cgcgtctggc tcacgctgag ttaagcttta cctacgatca atttccgcag gaaggccagg
     1561 gaaaccaggt acaccgcggt gcctaccgcg atccagtaac ccggctgcag ggcggtgatc
     1621 acataactct cggtgtgctc gttcatactg atgaacacat ttccctggag cactaccccg
     1681 tagaaatcgg cggagcggat cgagacaacg atcgggaata cgacgacaaa aacgacgtaa
     1741 gccaggccgg ctaacccgat caagatctgg gggacggata attttagttt gcccagcgag
     1801 actttgtcct ggctatcggt aaacaagctg tagagcagca gccagacaca caccgccagg
     1861 tacacccaca tgagggggaa gaaccagacg ggcatttcag ccccaacgag ccactgcgga
     1921 tagtctcgca gggttccact gatgatcatt ttgtacgggt agatcgcgac gccgatttca
     1981 tccagcgtct cgatgtatgc ggaccaccag ctctggaacc aagaatagac catcccggca
     2041 gctccggcca gggtcaggag ccggaagatc caggaacgga cgctgctcgc ctgttcggta
     2101 gcagtgaggc tcgccggtcc gctgacactt tcggtattgt tcataatcga ctttcctcca
     2161 tttccatatc acacggttac cctacagaac agggtcaccc tacagaacag ggttaccccc
     2221 atcttaaggg gcgaaccttg ttcagaccgg ggtgagattc tgttaggggt cagaatctca
     2281 cccctcgttc ggcaaagacc tctcccgggg caggtcaggc taacgcgatg tcgcccaggt
     2341 tgacgtcttc ctcggtggaa atagcgtcga tggctttggt cggcaagcca gcagcttcga
     2401 tggtgactgc atacgaccca acttccaggc cttcaaacca gaaatcgcca aactcgtcgg
     2461 tggtgaccgt cgccgtgctg tcgccagcca gggtgcagtt ggcgccgatg acgacttctt
     2521 cggtcgccgg gtcgtacacg gtgccggcaa tgaagcgctt gggcagattg aggtagaaga
     2581 ctcttggctt cgggttatcg ccaaattttg gctcgatgac ttcggcctga gaaatcagcg
     2641 ccgctaagtc ggcttcttcg ccaaaggtca gcgcgccggt tacgcaggca tccgtgcagc
     2701 gcggttctga aatcggccag ccgcgatcga gcagatgggc acaaccggtg cacttctggg
     2761 cgatgcgcag ggcacgatta taagagatgg cgccgtaggg gcaggaatcc acgcacagct
     2821 ggcagccatt gcatttcgca gggtcgatca gcaccaggcc atcgtcgcgc ttgctgatgg
     2881 cgccgtggac acaggcggtg acacaggggc ctgagtcgca gtgcatgcac agggtgggga
     2941 cgtaagaaac cttcaccttg ggcagcgtgc cccggatgta ttcgttctgc ttgtaccaga
     3001 actggccggt ttccgccatt ggtttggcat atggcatcca gtcgttaccg gcgtgctcgt
     3061 ctttgcaggc aacctggcag ctccggcagc cattgcattt tgcagcatca aataaaaagg
     3121 ctttcattat gcagctcctt cgatccagcc agtgaagaac ggtccataag ccgggtcata
     3181 atctctcgca aatgcttcgg gataggattc acgccactcc tgcatctgag ccccggtcac
     3241 tttttcgatc tgaaccagga agtgcgtgca ggccaggcca tatacattgc gcgagaagga
     3301 agatttggga ttgatacagt tgggattgcc accccggtgc agctcacccg cgatgatatg
     3361 gtgcccaccg ccggctttct cgaagcgcag tgcgccaggg atgatccgct ggctgatcac
     3421 cgcaccgccg agaacagcgc cgcgctcatt aaagactttg acaatgtcgc cgtcctggat
     3481 accgcgttca tcggcatcct tggggctgat ccagaccggt gcataggcat agccatccca
     3541 ggcggtgatc ttttcaattt cacgcgtcca ggggacgtcg gagaacatgg agtggtgttt
     3601 ccaggtcgag gtatccgata cgatcaccag cggataggtc ttcgcccgtt cgctggtcag
     3661 gtcctcgtcg tgggtccagc catcggcttc cgggccgcca atcacgtagt gggcaacggg
     3721 cgggcgctct ttatcgtcgg ggaagttctc tttcaacagg gacgattcga tctcgagcag
     3781 gccggtaggc gtcttcaaag gattggcaac cggatcgtcg tagaatttct tgtacgccgg
     3841 ttcgcgatcg taccacttcg gatcgggtgt ctgggggaaa taaccttttt cgaccagctt
     3901 ttcccaggag accagatcgg ttatttcgga ggcagcgtag gcagccttga ggcgttcctg
     3961 catcatctca tcgtagggct tgccgccggt gagcttgtcg taccagccta aagccttggc
     4021 aacttccagg actgcagcga ggtccgttcg ggcttcgccg atcggcgcca tcaccggctc
     4081 ggtgcgcagg gtgatgctgt tgaagatatc ccgggtggtg ttgatgtcgg gcgcttcggg
     4141 tgcggtggca atgggcagga tgatgtctga gaagaccagg ccatcttcca tccacattgt
     4201 ctggtggaca atgcattcca gtttctcgct ctgcattgct ttttgaacgc caaagcccca
     4261 ctggtggctg ccggtgtagg atgcactggt gccccagatc atatggactt cggatttacc
     4321 cggcatggga taggtgaatt tctggaactg cttgccgacc gggtaaaact ctaccggtgg
     4381 attgagcaca gcctgcgcaa aataaccgcg cgggaaagcc tgccgctctt cgggcatgac
     4441 cacatcacct tccatagccg tcgaccagat cgtatcgggg tctgccccac ccgcactggg
     4501 tgattgggcc gcgccgccga tggccgattg gagctggtgc agggtgtgct tgccgggtcc
     4561 acccaggcct tgcatacatt gcaggtaaag ctggatgcgc tgggggttat cggcataaat
     4621 tgtgcgcccg actgccccac caccggagcg gccatagccg atcgaggtgg gggtgttgta
     4681 c
//
LOCUS       DATMXK010000446         3407 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_107518, whole genome shotgun sequence.
ACCESSION   DATMXK010000446 DATMXK010000000
VERSION     DATMXK010000446.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3407)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3407)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3407
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107518"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..350
                     /locus_tag="VN363_08270"
     CDS             <1..350
                     /locus_tag="VN363_08270"
                     /inference="COORDINATES: protein motif:HMM:NF016144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GrpB family protein"
                     /protein_id="HWQ84545.1"
                     /translation="QRAGIASDHVPPGYSGPESDWEKWYFHPPASQRPTHLHVRRAGA
                     PNQRYPLLVRDYLRAHPATAAAYAELKRRLAAGLADPHTYPDVKDPASDLIYLAAEEW
                     STRTGWKPWMPDA"
     gene            771..1568
                     /locus_tag="VN363_08275"
     CDS             771..1568
                     /locus_tag="VN363_08275"
                     /EC_number="3.4.21.-"
                     /inference="COORDINATES: protein motif:HMM:NF012318.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006508 - proteolysis [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trypsin-like serine protease"
                     /protein_id="HWQ84546.1"
                     /translation="MKNKLFVPVLVLLAALLAVTPALAITFGELDGERHPNVGALLVA
                     WPYPGGPIDIVCSGTLISPTVFLTAAHCVNWMPGDGIPADDVYVTFDSVYEIGVSTIY
                     PGTYFVNPNYWPNNSDVNDVAVIVLDEPIDWITPATLPTAGLLDEMKAAGALKGQEFV
                     TVGYGTVRDDKTGGFHPNYWEGARRFTTGSFNALTPGWLKISMNPSTGDGGTCYGDSG
                     GPHFLGDSNLVVSVTVTGDAPCRATDDTYRLDIPSAREYLAQFVDLP"
     gene            complement(1726..2223)
                     /locus_tag="VN363_08280"
     CDS             complement(1726..2223)
                     /locus_tag="VN363_08280"
                     /inference="COORDINATES: protein motif:HMM:NF023144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="maleylpyruvate isomerase N-terminal
                     domain-containing protein"
                     /protein_id="HWQ84547.1"
                     /translation="MTLDPTYVEQNRLSTQRMRDIAARLSDAELLTPVGEHWTVAIVF
                     AHLAFWDRRVMYVLDGTARDQKLFVPEIDIFVNDLSLPLWAAIPPREAVRIGLETAAD
                     LDRRLEVYDPALLEKIYAYNPRWVVRALHRSDHLDEAEAALGWPAPGISKTSGSRSNA
                     AAPTL"
     gene            2390..>3407
                     /locus_tag="VN363_08285"
     CDS             2390..>3407
                     /locus_tag="VN363_08285"
                     /inference="COORDINATES: protein motif:HMM:NF037527.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84548.1"
                     /translation="MDQITRIELHNLLAEREGWCVSLYTPTLRAGHETEQNPLRLKNL
                     LQEAETRLLAEGLRTPDVRVLLKPAEDLLVDPTFWRAQSDGLAVFSAAGEFYTYRLPL
                     AFEAQLVVSHRFHIKPLLPFFANDGHFYILALSQNEVRLLQGTRHTVSPINLENVTGS
                     LREALKFDSFQKEIQMHGGGMVGGGAPGGGSQAGIFHGNDPADEDKDRLSGWFKKLDT
                     ELQPVLEADHSPLVLAGVEYLLSLYREANSYPNVLDQDIRGNPEMLKPAGLHERAWGL
                     VEPVFRRQQEAAIAQFHELASKGRTTTDLKKALKSAQEGRIASLFVPLGVEVWGSFDP
                     DSQKA"
BASE COUNT          712 a          982 c         1052 g          661 t
ORIGIN      
        1 cgcaaagagc aggcatcgcc agcgatcatg tcccgccggg ctacagcggg ccagaatctg
       61 actgggagaa gtggtatttc cacccgcccg cgagccagcg ccccactcac ctgcacgtga
      121 ggcgggccgg tgcgcccaac cagcgctatc cgctgctggt tcgcgattat ctgcgcgccc
      181 accccgccac ggcggcggcg tatgccgagc tcaaacgccg cctggcagcc ggcctggccg
      241 acccacatac ctacccggat gtgaaagacc cggcctccga cctgatctac ctggcagcgg
      301 aggagtggtc gacccgcaca ggttggaaac cgtggatgcc agatgcctga gggcgggttt
      361 cccgccatca atggaagccg tggatgccag atgcttcggt caaccgcaga tggcgcagat
      421 gggcgcagat gagttcaagt agaacaggat caattcttac agccaggtcc gatttgatcc
      481 gatatcctga agatcctggc catccatgtt cattcttcgg ttttctctgc gtttaatcgt
      541 ccgcagatga cgcagatggg aaccgtcagg tcgaacaggg aactgcaggc aagacagaat
      601 catttctgcc tggacgccct ggctgctgga tagcagaagc cagattatgt tgactcattc
      661 agtctgattt tgctataatc aagctgtggt ttgctccccg ggggatggga aggggaagga
      721 tgaagtgtag ttttgggaca aattcagagc tctgtgaaaa ggagaattgg atgaagaaca
      781 agctgtttgt accggtcctc gtgctgctgg cagccctgct ggcggtcacc cccgccctgg
      841 caatcacctt tggtgagctg gatggcgagc ggcaccccaa tgtaggcgcc ctgctggtag
      901 cctggcccta tcccggcggg ccaatcgata tcgtgtgcag cggcacgctc atctcgccca
      961 cggtcttcct gaccgcggcc cactgcgtga actggatgcc tggcgacggg atcccggcgg
     1021 atgatgtata tgtcaccttc gattccgttt acgagatcgg ggtgtcaacg atctatcccg
     1081 gcacctattt cgtgaacccc aactactggc ccaacaattc cgatgtaaac gatgtggcgg
     1141 taattgtgct ggacgagccg attgattgga tcacccccgc cacgctgccc accgccggcc
     1201 tgctggatga aatgaaggct gccggtgcgc tcaagggcca ggaattcgtc acggtgggct
     1261 acggcacggt gcgggacgat aaaaccggcg ggttccaccc gaattactgg gaaggggcgc
     1321 ggcgcttcac taccggcagc ttcaatgcat tgaccccggg ctggctgaag atctcaatga
     1381 acccctccac cggggacggc ggcacctgct acggtgattc aggcggcccg cacttcctgg
     1441 gcgacagtaa tctggtcgta tcggtcaccg tgaccggcga tgctccctgc cgggcgacgg
     1501 acgataccta ccggctggat atcccctcgg cgcgggagta cctggcgcaa ttcgtggatc
     1561 tgccgtaaag gttgatttcg aagtttatct caaaacccca gggctgaaat cctccggatt
     1621 tcttagaccc gggggtttca gaggtaatga tagcttcaca agccaaacaa cctggagatc
     1681 ttcagggtca gggcgatttc tctttgaccg gaatctatat cccggtcaaa gagttggagc
     1741 cgctgcattt gaacggctcc ccgaagtttt cgaaatccca ggcgcaggcc atcccaaagc
     1801 cgcttcggcc tcatccagat ggtcgctgcg gtgaagggcg cgcaccaccc agcgcgggtt
     1861 gtaggcatag atcttttcaa gaagagccgg atcgtaaact tccaggcgcc ggtccaggtc
     1921 ggcggcagtc tcgaggccga tgcgcactgc ctcgcggggt gggatggcag cccaaagcgg
     1981 gagagataaa tcgttgacga agatatcgat ctcggggaca aaaagcttct ggtcgcgggc
     2041 ggtgccatcc aggacgtaca tcacccgccg gtcccaaaaa gcgaggtggg cgaagacgat
     2101 ggcgacggtc cagtgttcgc caacgggggt gagcagctcg gcatcgctga ggcgggcggc
     2161 aatgtcgcgc atgcgttggg tggaaaggcg gttttgctca acataggtcg ggtcaagggt
     2221 cataggaaag gctcctgaag gtgttggcgg aatcttgctg cagcctgatc atacaacgaa
     2281 attgccacaa ggacataggt cgattcgatc cgaattaaat agtcccgttt tcaggtcaga
     2341 tccatcttct acctgtataa tgtgggcaca aacctgaaag gagactgaca tggatcagat
     2401 aacccggatt gagctgcaca atttactcgc cgagcgagaa ggctggtgcg tctcgctgta
     2461 tacacccacg ctgcgcgccg gtcacgagac ggagcagaat ccgctgcgcc tcaaaaatct
     2521 cctgcaggag gcggaaaccc gcctgctggc tgagggactg cgcaccccgg atgtgcgcgt
     2581 cctgctcaag ccggcggagg atctgctcgt cgatccgact ttctggcgcg cccagagcga
     2641 tggactggcc gttttcagcg cggccgggga gttttacact taccggctgc cgctggcatt
     2701 tgaagcccag ctggtggtca gccaccgctt ccacatcaaa cccctgctgc cattcttcgc
     2761 caatgatggc catttttaca tcctggcgct cagccagaac gaagtccgcc tgctgcaggg
     2821 gacccgccac acggtcagtc cgattaatct ggagaacgtc acgggcagcc tgcgggaggc
     2881 gctgaaattt gacagcttcc agaaggagat acagatgcac ggcggtggga tggttggcgg
     2941 cggtgccccg ggaggcggca gtcaggcggg tatcttccac ggcaacgatc ccgcggatga
     3001 ggataaagac cgcttgagcg gctggtttaa aaagctggac acggagctgc agccagtgct
     3061 ggaagccgat cactcgccgc tggtgctggc cggggtggaa tacctgcttt ctctctaccg
     3121 ggaggcgaat tcgtacccga atgtgctgga ccaggatatc cgcggcaacc cggaaatgct
     3181 caagccggcc gggctgcacg agcgcgcctg ggggctggtc gagccggttt tccgccgcca
     3241 gcaagaagcc gccatcgccc agttccacga gcttgccagc aaagggcgga cgacaactga
     3301 tctcaagaaa gccttgaaat cagcccagga ggggcggatc gccagcctgt ttgtgcctct
     3361 gggtgtggaa gtgtggggca gcttcgatcc ggacagccag aaagccc
//
LOCUS       DATMXK010000447         2512 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_74390, whole genome shotgun sequence.
ACCESSION   DATMXK010000447 DATMXK010000000
VERSION     DATMXK010000447.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2512)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2512)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2512
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74390"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            77..1888
                     /locus_tag="VN363_08290"
     CDS             77..1888
                     /locus_tag="VN363_08290"
                     /inference="COORDINATES: protein motif:HMM:NF013481.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aldehyde ferredoxin oxidoreductase family
                     protein"
                     /protein_id="HWQ84549.1"
                     /translation="MLGGYANRMAHVDLSTGKVEYKAIPEEWARKYIGARGLGVKYVF
                     ENGPQVDPLSPDNILCFMNGPLTGSEANMSGRMAIVTKSPLTNTVTDSHHGGWSAARL
                     RWAGFDGLIFKGKADKPVYAYVHDDQVELVDASPVWGKGVHAAVAYFNDLYGEKDLSV
                     IAIGQAGEHQVKYAAWINENDRASGRGGTGCVGGAKQLKAIVIKSEKKITKAADREAW
                     KEAHKRALATIMDEKNITSPRKGALSVYGTNVLTNITNNMGGLPTRNSQLTSFGDLAE
                     KTGGEYVKEHYLVNDPTCHACPVACKKEVEAKAGPFKGLHMESTEYESVWVFGANAGN
                     DDMNGIIKLIDQCNDYGMDTIEAGQALSAYMEATQQGYTNGDGLKWADVMGMVAALEK
                     TASRDGIGDVLAEGAVGVAKHYGHPELSMTVKGMAIPAYDPRSMKGMGLGYATSNRGA
                     CHVRGYSPASELGLIPLKTDPLEWKGKGKLIKLLQDLHAFSDSMDLCLFSAFAEGGDE
                     YASQYAAIVGVPFTADDVLETGERIYNLERYYNNLAGFREGSDYLPERFLKEPSTLLA
                     SKGHICELPEMLDEYYEARGWVDGVVPEAKLKELAIV"
     gene            complement(1993..2250)
                     /locus_tag="VN363_08295"
     CDS             complement(1993..2250)
                     /locus_tag="VN363_08295"
                     /inference="COORDINATES: protein motif:HMM:NF020751.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF1952 domain-containing protein"
                     /protein_id="HWQ84550.1"
                     /translation="MIQQTRELRGIPIWLLSAYLVEIGGAELDSGVIAGQGWKIQLQQ
                     LADFQVGSIRVGQVRLDLEAEPAVYPALNEALDKKLLRAGG"
     gene            complement(2247..>2512)
                     /locus_tag="VN363_08300"
     CDS             complement(2247..>2512)
                     /locus_tag="VN363_08300"
                     /inference="COORDINATES: protein motif:HMM:TIGR01687.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWQ84551.1"
                     /translation="LRQIVGGRSAEFDLPENATVGQLLAEMIRVYPALRQELLDEHGE
                     LYPHVHYFINGRDVPYLGEGMETPLKQDDIVTVFPAVGGGIAQ"
BASE COUNT          572 a          697 c          757 g          486 t
ORIGIN      
        1 tcaccgctac aaatccaaat gtttgctttt ggttcctgcc tcaaatataa acaggaagta
       61 aataaggaga aaaatcatgc tcggtggtta tgctaatcgg atggcccatg tggatctgtc
      121 cactgggaaa gttgaataca aagccattcc tgaggaatgg gctagaaaat atatcggggc
      181 gcgcggcctg ggcgtcaagt atgtattcga gaatggaccg caagtggatc cgctatcgcc
      241 ggacaatatc ctgtgcttca tgaacggtcc gctgaccggg agcgaagcca acatgagcgg
      301 gcggatggcg atcgtgacca aatcccccct gaccaatacc gtcaccgaca gccaccacgg
      361 cggctggtcg gcggcgcgcc tgcgctgggc agggtttgat ggcttgattt tcaaggggaa
      421 agcggacaaa ccagtttacg cctatgtgca cgatgatcag gttgagctgg tcgatgcttc
      481 tccagtctgg ggcaagggcg tccatgctgc ggtagcgtat ttcaatgatc tctatggcga
      541 gaaggatctg agcgtgattg ccatcgggca ggctggtgaa caccaggtaa agtacgctgc
      601 ctggattaat gagaacgatc gcgccagcgg gcgcggtggc accggctgtg tgggcggagc
      661 caagcagctg aaagcgattg taattaagtc ggagaagaag atcaccaaag ctgccgatcg
      721 cgaagcctgg aaagaagctc acaagcgggc gctggcgacg atcatggacg agaaaaatat
      781 caccagcccg cgcaaaggtg ccctttcggt ctacggcacc aacgtcctga ccaatatcac
      841 caacaatatg ggtggtctgc ccacccgcaa cagccagcta acctcgttcg gcgatctggc
      901 ggagaagacc ggtggtgagt acgtcaaaga acactatctg gtcaatgatc ccacctgcca
      961 tgcctgcccg gttgcctgca agaaagaagt cgaagccaaa gccggcccct tcaaaggcct
     1021 gcacatggaa agcaccgaat acgagtcggt gtgggttttt ggcgccaatg ctggcaacga
     1081 tgatatgaac ggcatcatca agctgatcga ccagtgcaac gactacggca tggatacgat
     1141 cgaggccggt caggcgcttt cggcatacat ggaagccacc cagcagggtt acaccaacgg
     1201 cgatggcttg aaatgggctg atgtgatggg tatggtcgcc gcgctcgaga aaaccgcctc
     1261 ccgggatggg attggagacg tcctggctga aggcgcggtt ggcgtcgcca aacattatgg
     1321 tcaccccgaa ttatcgatga cggtcaaggg catggcgatc ccggcttacg atccgcgcag
     1381 tatgaagggc atgggcctgg ggtatgccac cagcaaccgc ggcgcctgcc atgtgcgtgg
     1441 ttacagcccg gccagcgagc tggggctgat cccgctgaaa acagatccat tggaatggaa
     1501 gggcaagggc aagctgatca agctgctcca ggacctgcac gccttctccg acagcatgga
     1561 tctgtgcctg ttcagcgctt ttgccgaagg cggcgatgaa tatgccagcc agtatgctgc
     1621 catcgtcggg gtgcctttca ccgccgatga tgtcctggaa accggtgagc gcatctacaa
     1681 cctggagcgc tactacaaca acctggcggg cttccgcgag ggcagcgact acctgccgga
     1741 gcggttcctg aaagagccct ccaccctcct ggcatcaaag ggccatatct gcgagctgcc
     1801 tgagatgctg gatgaatatt atgaagcgcg cggctgggtt gacggcgtcg ttcctgaagc
     1861 caagctcaaa gagctggcga tcgtttaatc aatcctggat cgatcgggta taaggattca
     1921 ggcatcgtaa aggggctgtc cgttaagcag ggcagcccct ttctatgctg gctggatcgc
     1981 gcggcagctc agttagcccc cggcgcgcag cagtttctta tcgagagctt cgttgagagc
     2041 gggatagacc gccggttcag cctccaggtc caggcgcacc tggcccaccc ggatcgatcc
     2101 tacctgaaaa tcagccagct gctggagctg gatcttccag ccctgacccg cgatcacacc
     2161 gctgtccagc tcggccccgc cgatctccac cagataggcg ctgagcagcc agatcgggat
     2221 gccgcgcagc tcgcgggtct gttggatcac tgcgcaatgc cgccgccgac ggctggaaaa
     2281 acggtcacaa tatcatcctg tttgagcggg gtttccatac cctcacccag gtaagggacg
     2341 tcccggccat tgatgaagta atggacgtgt ggatagagct cgccgtgctc gtccagcaat
     2401 tcctggcgca gggcaggata gacgcgaatc atctcggcga ggagctgccc gaccgtggcg
     2461 ttttccggaa ggtcgaattc tgcgctccgc cccccaacaa tctggcgtaa tg
//
LOCUS       DATMXK010000448         2181 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_105151, whole genome shotgun sequence.
ACCESSION   DATMXK010000448 DATMXK010000000
VERSION     DATMXK010000448.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2181)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2181)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2181
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_105151"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..483)
                     /locus_tag="VN363_08305"
     CDS             complement(<1..483)
                     /locus_tag="VN363_08305"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 2 protein"
                     /protein_id="HWQ84552.1"
                     /translation="MPSVTVIIANWNGKAYLPTCLEALAAQTYADFEILVIDNGSNDD
                     SVDTIQVDWPAVRLFKLENNQGFARANNTGAKLAEGEWIAFLNNDAYPEPGWLEALVA
                     ATHVNPACAAFASRIHLVAPPELLDSTGDIYHISGNAWHRGYRQPAQEFTPPAGFVFS
                     A"
     gene            complement(503..1861)
                     /locus_tag="VN363_08310"
     CDS             complement(503..1861)
                     /locus_tag="VN363_08310"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family 4 protein"
                     /protein_id="HWQ84553.1"
                     /translation="MKILLVANGYPPTAVGGVETYTAELALSLSGFGDQISVFCRESN
                     FSKPDYGLSTENQGQISITRVVNDHKRATSFRQTFIDPQIEEIFTRYILAEKPDLIHF
                     NHLIGLSARLPLIAAEQNIPAVITLHDFWPICHQVKLIDWQGRICPGPAYAVDCPTCA
                     SGGSAQQDGALIEKATRLVKRILPARVRRTIRSAMPEGEALPPAMISSPEIFRERLDL
                     FTRAVLAAQRILVPSEYSRTELGKNGLPRERIQVIPLGIAVQASRSPRESLSGSLTFA
                     TIGPIQPIKGTDIAIKAFSKVPGEQLRLNIYGRHDLFPRDHIKQVLKLAQADPRVSIL
                     GPFDPRDRVSIFNAIDVLIVPSPAPETFSLAAHEALAMGKPVLASRIGALPEIIQDGV
                     NGFLFEPGNDTRLAEFITLLASQPGILEKLSMPGTTPILTSIEHAREIKNIYHQVVRS
                     GG"
     gene            complement(1858..>2181)
                     /locus_tag="VN363_08315"
     CDS             complement(1858..>2181)
                     /locus_tag="VN363_08315"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012744.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016757 - glycosyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ84554.1"
                     /translation="VYASFDILVVPSRAPETFSLVTREALARGKPVIAARIGAFPEII
                     QDGVNGFLFPPGDVQALAGLMTGFTNDRTLINQLSIPGPAQTLETTEHGEQIQMIYRQ
                     ISGNL"
BASE COUNT          520 a          564 c          608 g          489 t
ORIGIN      
        1 ggcgctgaac acaaacccgg ctggcggagt gaactcttga gccggctgcc ggtagcctcg
       61 gtgccaggcg tttccgctga tgtggtagat atctcccgta gaatcaagta attcaggcgg
      121 cgcgacaagg tggatgcgag aggcaaaagc ggcacaagcc ggattgacat gggtggcagc
      181 caccaaagct tccagccatc cgggttcagg gtaagcgtca ttgttgagga aagcgatcca
      241 ttctccctca gccagctttg ctcccgtgtt gttcgccctg gcaaaacctt gattattttc
      301 tagcttgaac aaccttacag ccggccaatc gacctggatt gtgtctacag agtcatcatt
      361 agatccatta tcgatgacca ggatctcgaa atcagcgtag gtctgcgccg ccagagcctc
      421 caggcaggtg ggcaggtatg cttttccatt ccaattggct atgatcaccg ttacggatgg
      481 cattttagtc gccacctgag tcctatccac ccgacctcac gacctggtgg taaatatttt
      541 ttatctcccg cgcatgctct atagatgtga ggatcggcgt tgttccgggc atcgacagct
      601 tttccagtat accgggttgg ctggcgagca gggtgatgaa ttcagccagc cgggtatcat
      661 tgcctggctc aaagagaaag ccattgaccc catcctggat aatttcaggc aaagccccga
      721 tcctggacgc cagaactggt tttcccatgg ctaaagcttc atgtgcagcc agcgaaaatg
      781 tctcaggagc cggggaaggg acaatcaaaa cgtcgatagc attaaaaatc gacacccgat
      841 cccgcgggtc aaaaggtccc agaattgata cccgcggatc agcctgagcc aacttcaaaa
      901 cctgcttgat gtggtcgcgt ggaaacaagt catggcggcc ataaatgttc aaacgcagct
      961 gctcgcctgg cactttactg aaagccttga tagcaatatc agttccctta atgggttgga
     1021 tcggaccgat tgttgcgaaa gtcagtgaac cggacaaaga ctccctgggc gagcgactgg
     1081 cttgcacggc aattcccagg ggtatgacct gaatccgctc gcgcggcagg ccatttttgc
     1141 cgagctcggt ccgggaatat tcggatggga cgaggatccg ttgagcagcc aggactgctc
     1201 gggtaaatag atccagccgt tcgcggaaaa tttctggcga agagatcatt gcaggcggaa
     1261 gagcttcccc ttcaggcata gcggagcgta tggtgcgccg cacgcgggca ggcaggatgc
     1321 gtttaaccaa acgggtggct ttttcaatca gggcgccatc ctgctgagct gacccgccgg
     1381 atgcacaggt ggggcaatca acagcgtatg ccggcccggg gcagattctc ccctgccagt
     1441 ctatcagctt aacctgatgg cagatcggcc agaaatcgtg cagagttatg acagcaggaa
     1501 tgttctgttc agcagcgatg agcggcagcc gggctgacag cccgatgagg tgattgaaat
     1561 ggatcaaatc gggcttttca gccaggatat accgggtaaa aatttcttcg atctgcggat
     1621 cgataaaggt ctggcggaat gaagtagctc gcttatggtc gttcacaacc cgggtgatgc
     1681 ttatctggcc ctgattttcg gtagatagcc catagtccgg ttttgagaaa ttcgactcac
     1741 ggcaaaacac acttatctga tccccaaagc cactcaggga aagagctaat tctgccgtat
     1801 aggtttctac accgccaacc gctgtgggag ggtacccgtt ggcgaccagc aggattttca
     1861 taaattacct gagatctggc gatagatcat ctggatttgt tccccatgtt ctgtcgtttc
     1921 cagcgtttga gcagggccgg gtattgacag ctgattaatc agggtgcgat cgtttgtgaa
     1981 gccagtcatg agaccggcta aagcctggac gtcgcctgga ggaaataaga aaccgttgac
     2041 cccatcctga atgatctcag ggaaagctcc aatccgggct gcgatcacag gtttgcctcg
     2101 cgccagtgct tcccgcgtca ccagtgagaa agtttcagga gcgcgggatg gcacgaccaa
     2161 aatatcaaac gaggcgtaaa c
//
LOCUS       DATMXK010000449         2603 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_152861, whole genome shotgun sequence.
ACCESSION   DATMXK010000449 DATMXK010000000
VERSION     DATMXK010000449.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2603)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2603)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2603
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_152861"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1511
                     /locus_tag="VN363_08320"
     CDS             <1..1511
                     /locus_tag="VN363_08320"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84555.1"
                     /translation="LILSLMQTRNLVIWITFLAVFAMAATFAVDTDTWWHLRAGEWMV
                     ENRQILQVDHFSHTRAGAPWRYPGWLVEIAMYLLFDSAGPGGLNLWTAGMVTLAFAFV
                     WRVQSGNPYLRAFITILGAAASGVYWAARPYLVTFVLTAVFLWILESWRWRESEKNPR
                     RLWLLPVLMVGWANSHGGFFTGFLLLAVYLAGEGWDWLSAKILARRGLQPATGAGDHL
                     RRLRMLGAVSLVTLLAACLSPVGPRLLLYPFQTVEIASLQAYIQEWQPPDFHNLSMQP
                     FLWLFLLSLGAIGASRRRLALTDFLLLAGFGYLGFLAARNIALFALAALPPLSRHSAD
                     ALQIAGRALRLKPLETQTPPHLRRLNLALVGLVGVAVLARALPVLPATANEDHFRQQL
                     PVAAAQAVMQAAPEGNLFNSYNYGGYLVWALRDYPVFVDGRTDLYSDEIISEWLAAVR
                     AEPGWEEILDRREIGVILIEPETPLTAVLAGQPGWEELYRDEIAVVYRQVQP"
     gene            complement(1622..>2603)
                     /locus_tag="VN363_08325"
     CDS             complement(1622..>2603)
                     /locus_tag="VN363_08325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84556.1"
                     /translation="SYYWRVQPRYLAGGEYFGVWSQGARFERQGFVPENLQVSVTHAT
                     PTFSWDMVEGAQSYDLQVDNDPNFGLAEININTSQASFTPTDTLPNGVYYWRVRVQRW
                     GNISNEWSPSQSFNLTLLAPTGLTPHDPGATQVFGQAPTLCWQPVLVNDNDNHPVLAA
                     YRYRVQILFNSVPVETIETEQTCYTPTNGYADGLYSWRVAMIDGNNRLGDFSALATFT
                     KQYPITTLIDPVNESVATTPTFYWTAVQGAANYQLEISLSPTFGAIYDAAVTNNIRYT
                     PNRLYQTPQTYYWRVAMIDQNGRMGPFTDAQIILGPSDLPNKLFLPALKH"
BASE COUNT          455 a          761 c          819 g          568 t
ORIGIN      
        1 gcttaatatt gagcctgatg cagacccgaa acctggtcat ctggataaca tttctggcgg
       61 tattcgccat ggcagccaca ttcgctgtgg ataccgacac ctggtggcac ctgcgcgccg
      121 gggaatggat ggttgaaaat cgccagatcc tgcaggtgga tcatttctcg cacactcgcg
      181 ccggtgcgcc gtggcgctat cccggctggt tggtcgagat cgccatgtat ctgctcttcg
      241 attcagcggg tccggggggt ctgaacctgt ggacggcggg aatggtgacc ctggcgtttg
      301 ccttcgtctg gcgtgtccag tccggcaacc catacctgcg cgcctttatc acgatcctcg
      361 gagcggctgc ctccggggtc tactgggcgg cgcggcccta cctggtcact tttgtgctca
      421 ccgccgtgtt tctgtggatc cttgaatcct ggcgctggcg agagagcgaa aaaaaccccc
      481 gccggctgtg gctgctgccg gtcctgatgg tcggatgggc gaacagccac ggcggatttt
      541 ttaccggatt cctgctgctg gctgtatacc tggccggcga aggttgggac tggctgagcg
      601 cgaaaatcct ggcgcgccgc gggctgcagc ctgcaaccgg cgcaggtgat catctccggc
      661 gcctgcggat gctgggcgcc gtcagtctgg ttaccctgct ggcagcctgc ctgtcaccgg
      721 tcgggccgcg cctgctgctg tatcccttcc agactgtgga aatcgcctcc ttgcaggcgt
      781 atattcaaga gtggcagccg cccgatttcc acaacctgag catgcagcct ttcttatggc
      841 tgttcctgct cagcctggga gcgatcggtg cttcccggcg ccggctggcg ctgaccgatt
      901 tcctgctcct ggcaggcttt ggttaccttg gcttcctggc ggcgcgcaat atcgccctgt
      961 ttgccctggc agccctccca ccgctcagcc gccacagcgc ggatgccctg cagattgcgg
     1021 ggcgcgccct gcgcctcaag cccttggaga cgcagacacc gccccacctg cgccgcctga
     1081 acctggctct ggtgggcctg gtaggggtgg cggtgctggc ccgtgccctg ccggtgctgc
     1141 cggcaaccgc caacgaggac cattttcgcc agcagctgcc ggtggcagcc gcccaggctg
     1201 tgatgcaggc agccccagag ggtaacctgt tcaattccta taactacggc ggctacctcg
     1261 tctgggcgct gcgcgactac ccggtcttcg ttgatgggcg caccgacctg tacagcgatg
     1321 agattatcag cgaatggctg gcggcggtgc gcgccgagcc gggctgggaa gaaatcctcg
     1381 atcggcgcga gatcggcgtg atcctgatcg aacccgagac acccctgaca gccgtactgg
     1441 cagggcagcc gggctgggaa gagctgtacc gcgatgaaat cgcggtggtt taccggcagg
     1501 tgcagccata attcactgaa atattaaaga actcacccgc agaggctgac tctgcgggtg
     1561 agttttgatt ctccggggct tctctctaaa ggagatgcgc ctgtgcgtga agtgctgcca
     1621 gctagtgctt caaggctggc aggaacagct tattgggtaa atctgaagga ccaaggataa
     1681 tctgggcgtc cgtaaagggt cccattctcc cattttgatc gatcatcgcc acccgccagt
     1741 agtatgtctg gggagtctgg tacaggcggt tgggtgtgta gcggatattg ttggtgaccg
     1801 ccgcgtcgta gattgcccca aaggttggag agagtgaaat ttcgagctgg taattggctg
     1861 ctccctggac cgcggtccag taaaaggtcg gcgttgtcgc aaccgattcg ttcaccggat
     1921 caattaacgt cgttatcggg tactgtttgg taaatgtagc cagcgcgctg aagtcaccca
     1981 ggcgattgtt cccgtcgatc atcgccaccc gccagctgta aagaccatcc gcatagccgt
     2041 tggtgggagt gtagcaggtt tgctcggttt cgatcgtctc caccggcacg ctgttgaaga
     2101 ggatttgcac ccggtagcga taggcggcca gtaccggatg gttatcgtta tcgttcacca
     2161 gcaccggctg ccagcacagc gtcggcgcct ggccgaagac ctgggtcgca cccggatcgt
     2221 ggggcgtcaa tcctgtaggg gcgagcaggg tgagattgaa gctctggctg ggcgaccatt
     2281 cgttggatat atttccccag cgctgcacgc gcacccgcca gtaatacacg ccattgggca
     2341 gggtatcggt gggggtgaac gaggcctggc tggtattgat attgatttca gccaagccga
     2401 agttagggtc attatctacc tgaaggtcat agctctgggc gccttccacc atatcccagg
     2461 agaatgttgg ggttgcatgg gtgaccgaga cctgtaaatt ctccgggaca aagccctggc
     2521 gttcgaagcg cgctccctgc gaccagacgc cgaaatattc cccgccagcc aggtatcttg
     2581 gttggacgcg ccagtagtag ctg
//
LOCUS       DATMXK010000450         2229 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_24669, whole genome shotgun sequence.
ACCESSION   DATMXK010000450 DATMXK010000000
VERSION     DATMXK010000450.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2229)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2229)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2229
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_24669"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..686)
                     /locus_tag="VN363_08330"
     CDS             complement(<1..686)
                     /locus_tag="VN363_08330"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84557.1"
                     /translation="MSFPSIALAATWNPRGETRRLELLLERLRWAYTGIYISLSPLPD
                     DPASQTELLDRLAGDKLKCQVNPDWSWGRYTAVQMALSSGAPWIHYVDLDRLLRWVEG
                     QPDEWAQTLSAIQGSDYLVIGRTKAAYATHPQALVQTEALSNTVISFLVGQQMDVSAG
                     SKGFSRQAASYVIENTMPGHALGTDGEWTVLLHRAGFKLGYIEVNGLDWESADRYQNR
                     AADPTRQRQAA"
     gene            complement(683..1072)
                     /locus_tag="VN363_08335"
     CDS             complement(683..1072)
                     /locus_tag="VN363_08335"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84558.1"
                     /translation="MAFRCPQCKTPASLGITSSIELPPDSRSDEITLQVVECSACGFG
                     GLAVYEEHRHAIQETDDWNHIGYWVSQDAVESVKEAIRSCPNPLQAQCKCSVHSSLSQ
                     RDIYGVWKGLLELERGHTFLMRMATAE"
     gene            complement(1077..>2229)
                     /locus_tag="VN363_08340"
     CDS             complement(1077..>2229)
                     /locus_tag="VN363_08340"
                     /inference="COORDINATES: protein motif:HMM:NF019869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006418 - tRNA aminoacylation for protein
                     translation [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="class I tRNA ligase family protein"
                     /protein_id="HWQ84559.1"
                     /translation="IYCPDCGEVAVPEDQLPVLLPEMTDFSPDGTGRSPLARVPEFIN
                     TTCPNCGKPAVRETDTMGGFACSSWYFLRFASPHENERPFAPEDIRYWLPVDLYVGGA
                     EHAVLHLLYARFWTMVLADEGLLPFREPFSSLRNQGQLLGPDGVRMSKSRGNVITPDA
                     IAASFGADALRVYEMFMAPFDQDIGWTQTGIQGAARFLNRIWTLYQETYFGSQSAAGS
                     DPDLERALHRTILQVTQRIDDFRLNTMISALMEFANLMGERWRAGAWQTATFHQALNK
                     FLVILAPSAPHISEELWHLTGHTDSIHRQSWPEASAELAAEKLVELPVQINGKLRATI
                     WLAADASPDQAQSDAIADPKIEQFLQHARIERVYYVPGKVINLIVKPGA"
BASE COUNT          516 a          632 c          595 g          486 t
ORIGIN      
        1 gcagcctggc gctggcgagt tggatccgca gcccggtttt gataccggtc agccgattcc
       61 cagtccagac cattcacttc aatataaccc agtttgaaac ctgcacgatg caagagaacc
      121 gtccactcgc catcggttcc cagggcatgc ccgggcatcg tattttcaat gacataactg
      181 gctgcctggc ggctgaatcc ttttgatcca gcactcacat ccatctgctg tccaaccaga
      241 aatgatatta ctgtattact caatgcctcg gtctgaacaa gagcctgcgg gtgggtagcg
      301 taggcggcct tagttcgtcc gatcaccagg taatcagatc cctggatcgc tgacaaagtc
      361 tgggcccact catccggctg gccttcaacc caacgcagca ggcgatccag atcaacataa
      421 tggatccagg gagcgccgct gctcaaagcc atttggactg ccgtgtagcg cccccatgac
      481 cagtctggat taacctggca tttgagctta tcacccgcca ggcgatccag taattctgtc
      541 tggctggcag gatcgtctgg gagaggggaa agtgaaatat agatgcctgt gtaagcccac
      601 cgcaggcgtt ctaaaaggag ctccaggcgt cgtgtctccc cgcgtggatt ccaggtggca
      661 gccagggcaa ttgaaggaaa actcattcgg cagtcgccat acgcatcagg aaagtatgcc
      721 ctcgctccag ctccaacaga cctttccaga cgccgtagat atccctctgg ctcaggctgg
      781 aatggacgct gcatttacac tgcgcctgca aaggattcgg gcaggaccgg atggcttctt
      841 tgacggattc tacggcatcc tgcgagaccc agtaaccgat atgattccaa tcatcggttt
      901 cctgaatggc gtgacgatgt tcttcgtaca ctgccaggcc gccaaatcca catgcagaac
      961 attccacaac ctgtaaagta atctcgtccg agcggctgtc aggtggcagc tcgatactgc
     1021 tcgtgatgcc cagactggcc ggtgttttac actgtggaca acgaaatgcc attcagctat
     1081 gctcctggct taacaatcag attgatgacc ttacctggaa cataatacac tctttcgatc
     1141 cgggcgtgtt ggaggaattg ctcgattttg ggatcggcaa tcgcgtcgga ttgggcctgg
     1201 tcaggtgaag catctgccgc cagccagatc gtagcccgca gcttgccgtt tatctgtacg
     1261 ggcagctcga ccagtttttc agccgccaac tcagctgaag cttcgggcca gctttggcgg
     1321 tggatgctgt ctgtatgtcc ggtcaaatgc cagagttcct cggagatatg cggtgcagac
     1381 ggcgcaagga taaccaagaa cttatttaat gcctggtgaa aagttgccgt ctgccaggct
     1441 cctgcacgcc agcgctcacc catcaggttg gcaaattcca tcagcgccga gatcatcgta
     1501 ttcaatcgaa aatcatcgat ccgttgagtc acctgcagga ttgtacggtg cagtgcacgc
     1561 tccagatccg ggtcactacc ggcggctgat tggctgccaa aataagtctc ctggtacaga
     1621 gtccagatcc gatttagaaa gcgggcagct ccctgaatgc ctgtctgggt ccacccgata
     1681 tcttgatcga aaggggccat aaacatctcg tacacccgta aggcatcagc tccaaagctg
     1741 gcggcgatag catctggagt gatgacattt ccccggctct ttgacatgcg cacaccatcc
     1801 gggcctaata actgaccctg gttgcgcaga ctggaaaatg gctcccggaa tggaagcaga
     1861 ccttcgtcgg cgaggaccat ggtccagaaa cgagcgtaca acagatgcaa aacagcatgc
     1921 tctgccccgc cgacatacag gtcaaccggt aaccagtagc ggatgtcttc cggggcaaag
     1981 gggcgctcgt tttcatgtgg gctggcgaag cgcagaaagt accaggagga acaggcaaat
     2041 ccccccatgg tgtcggtttc gcgtaccgcc ggttttccac agttagggca ggttgtattg
     2101 ataaactcag gtacccgcgc cagcggcgaa cgccccgtgc catccggact gaaatcggtc
     2161 atctcgggca gcaggactgg cagctgatct tcagggacag ccacttcacc gcaatcaggg
     2221 caataaatg
//
LOCUS       DATMXK010000451         4084 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_119290, whole genome shotgun sequence.
ACCESSION   DATMXK010000451 DATMXK010000000
VERSION     DATMXK010000451.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4084)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4084)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4084
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119290"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..201
                     /locus_tag="VN363_08345"
     CDS             <1..201
                     /locus_tag="VN363_08345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="N(G),N(G)-dimethylarginine
                     dimethylaminohydrolase"
                     /protein_id="HWQ84560.1"
                     /translation="RLLPVPEEEPWGANVLRIGERLWVQSAFPRTQEMVQSAGYAVEE
                     IDCGEFLKAEAGLTCLSLVYRI"
     gene            291..899
                     /locus_tag="VN363_08350"
     CDS             291..899
                     /locus_tag="VN363_08350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84561.1"
                     /translation="MRRIVGEVLSQLLNVPVLSGALITFFFFRLPNDLPNRLPGYGWA
                     LLFLSIIPLCSLFFYIPGKLKDKALVIRRQRIASFVFMIVSYPIGFLILRLTHAPKIF
                     EAMAITYTLVTLGLILLNFLLRYKASGHAAGVAGPVAALLFLFGLIATPFIALIPLTS
                     WARVNAKGHDIWQTLVGALLSFGITALVLWLYGFLPFQGLLH"
     gene            complement(841..2175)
                     /locus_tag="VN363_08355"
     CDS             complement(841..2175)
                     /locus_tag="VN363_08355"
                     /inference="COORDINATES: protein motif:HMM:NF019607.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PQQ-dependent sugar dehydrogenase"
                     /protein_id="HWQ84562.1"
                     /translation="MNQKPYFTHRIANAAKLLILAALIFNLGTVQIDSRAAQTLLPPL
                     EPILVTGGLTQPVHITHAGDGSGRLFIVEQRGAVRVFSGSLLTEPLLDIQDQVRSTGG
                     EEGLLSVAFPPGYAQKGYFYVYYTNLDGDNQVSRFHLGNSINSADPASEEVILLLPHP
                     TNSNHNGGQLAFGPDGYLYIGTGDGGGTGDPFDNAQNLDSLLGKILRIDVEPEPAMPP
                     TGDFNAFMPALHRGLLAVSPPAYHIPADNPFVSLAGARAEIWAYGLRNPWRFSFDRLT
                     GDLWIGDVGQAQWEEIDLQLQASKGGENYGWDVMEGTHCYGFTPCDPTGKILPIFEYD
                     HSGQRCSVTGGFVYRGSAYPDYFGTYFLADYCSREVWGLTLSGDVLHREAFPAIDQQV
                     TSFGEDENGEMYLTDYSAGAIYRLAPAQQQVSLFNAANPGMGESHTTTAPGR"
     gene            complement(2172..2984)
                     /locus_tag="VN363_08360"
     CDS             complement(2172..2984)
                     /locus_tag="VN363_08360"
                     /inference="COORDINATES: protein motif:HMM:NF016970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF692 family protein"
                     /protein_id="HWQ84563.1"
                     /translation="MDLAINYSTPAAALLEQGKIEFDRFKTMDWPDMIAAARVYRPVA
                     VHFGLQAGNGRIKTLDWGAVARMLDDTGTPHVNLHLEATSKDFPDISLELPSPAERDK
                     MFARMLKDVRLAVDQLGPERVIIENVPYRGRDGKVFRLAVEPATIQAIVAETGCGLLL
                     DISHARIAAHHLSMDAGDYMQLLPVQSLRELHFTGVHNLNGRLQDHLSILENDWSWLD
                     WVLERISQREWPRPWLLAFEYGGTGEKFAARTSPTVIATQLPILLKKVKETV"
     gene            complement(3088..>4084)
                     /gene="mltG"
                     /locus_tag="VN363_08365"
     CDS             complement(3088..>4084)
                     /gene="mltG"
                     /locus_tag="VN363_08365"
                     /inference="COORDINATES: protein motif:HMM:TIGR00247.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008932 - lytic endotransglycosylase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="endolytic transglycosylase MltG"
                     /protein_id="HWQ84564.1"
                     /translation="DSTEMYYLSAKLLSNADQLTQPRDPADEMHYFVIESGESVASIS
                     NRLQAEGYLSSPEAFRTFLFYAGLDTILQAGEHEVSAAMSAMQIAHELKDPTPARVTL
                     SILAGWRSEEIAAALPASGLGFTPADFLRIVKHPPSDLSLPAELPAGASLEGFLFPGN
                     YTIERRMGAEECISLVLVNFAAQVTPAIQQAFAGQGLSLYEAVTLASIVEREAVLYEE
                     MPAIASVFYNRLAAGMKLDTDPTVQYAAGYNPVQKTWWTNPLSSADLTINSPYNTYLN
                     PGLPPGPIANPGLAALEAVAYPAETPYYYFRAKCDNTGRHNFAETYQGHLDYACP"
BASE COUNT          825 a         1158 c         1212 g          889 t
ORIGIN      
        1 cggctgttgc ctgtgccgga agaggagccc tggggcgcca atgtgctgcg catcggggag
       61 cgcctctggg ttcaatctgc cttcccgcgc acccaggaga tggttcagag tgcgggttat
      121 gcggtagagg agatcgattg cggcgagttc ttgaaggctg aggccggact tacctgtttg
      181 agcctggtgt acaggatata attaatccta tccaacctga ctatgggagg cagcgaactg
      241 ctgttaacag acacatgaat ggacaggata ttcggaatgg cgctaaccgc gtgcgccgca
      301 tcgtcggcga ggtgctttcc cagctgctca atgtgccagt cttatccggg gcgctgatca
      361 ctttcttttt cttccgcctg cccaatgatc tgcccaaccg cctgccgggt tacggctggg
      421 cgctgctgtt tctcagtatt attccactgt gcagcctgtt cttctacatt cccggaaagc
      481 tgaaagacaa ggcactggtc atccgccgcc agcgcatcgc cagcttcgtg ttcatgatcg
      541 tcagctatcc catcggattt ctgatcctgc gcctgaccca tgcgccgaag atcttcgaag
      601 ccatggcaat cacctacacc ctggtcaccc tgggcctgat cctgctcaac ttcctcctgc
      661 gctacaaagc cagcgggcat gccgcaggtg tcgccgggcc ggtggcagcc ttgctgttcc
      721 tgtttggcct gatcgccacc ccgtttatcg ccctcattcc cctgacctcc tgggcgcggg
      781 tcaacgccaa aggccacgat atctggcaaa ccctggtcgg ggcgctgtta tcctttggga
      841 ttaccgccct ggtgctgtgg ttgtatggct ttctcccatt ccagggtttg ctgcattaaa
      901 taaactcacc tgctgttgtg ccggtgccag gcggtaaatt gccccggcgg aataatccgt
      961 caggtacatt tcaccattct cgtcttcgcc aaaactggta acctgctggt ctatcgcagg
     1021 gaatgcttcc ctgtgtaata catctccgct aagggtcagt ccccagactt cgcgcgagca
     1081 gtaatcggcc aggaaatacg tcccaaaata atccggatag gcgctgccgc gatagacgaa
     1141 tccgccggtg accgagcagc gctggcccga gtgatcgtat tcgaagatgg gtaagatttt
     1201 cccggtcggg tcacagggag taaaaccata acagtgggtt ccttccatca catcccaccc
     1261 gtaattttcg ccgcccttgc tcgcctggag ctgtaagtcg atctcctccc attgagcctg
     1321 gccgacatca ccaatccaca gatcgccagt caagcggtcg aagctgaagc gccacgggtt
     1381 gcgcagcccg tacgcccaga tctcggcacg cgcccctgcc aggctcacaa atggattgtc
     1441 ggcggggatg tggtaagccg gcgggctcac cgccagcagg ccgcggtgga gagctggcat
     1501 gaaggcatta aaatcgcctg tcggaggcat ggctggttcc ggctccacat cgatgcgcag
     1561 gatttttccc agcaggctgt ccaggttttg ggcgttatca aatgggtcgc cagtgccacc
     1621 gccgtccccg gtgccaatgt agagatatcc atccggtcca aatgccagct gcccgccgtt
     1681 gtggttgctg tttgtagggt gaggaagcag caggatcacc tcttcactgg ccggatcagc
     1741 cgagtttatg gagtttccca ggtggaagcg gctgacctgg ttatccccat cgaggttggt
     1801 gtaataaaca tagaaataac ctttctgagc atagccgggc gggaaagcca cgctgagcag
     1861 tccttcctca ccgccggttg agcgcacctg atcctggata tccagcaaag gctcagtgag
     1921 caggctgccc gaaaaaaccc ggactgcgcc gcgctgttcg acgataaaca gccgcccgct
     1981 gccatccccg gcatgggtga tatggaccgg ctgggtcaat ccgcccgtaa ccaggatggg
     2041 ctccagcggg ggcagcaggg tctgcgcggc gcgggaatcg atctggacag ttccaagatt
     2101 gaaaatgagc gccgccagaa tgagcagttt ggcggcgttg gcgattcgat gagtaaagta
     2161 aggtttttgg ttcatactgt ctcctttacc ttcttcaaga ggatcggtaa ttgggtcgcg
     2221 ataacggtcg ggctggtgcg agcggcgaat ttctcgccgg tcccgccgta ttcaaacgcc
     2281 agcagccagg gtcgcggcca ttctctctgg ctgatgcgct cgagcaccca gtcgagccag
     2341 gaccagtcat tctccaggat cgaaagatgg tcctgcaggc ggccgtttaa attgtgcact
     2401 ccggtaaagt gcagctcgcg caggctctgg accggcagga gctgcatgta atccccggca
     2461 tccatgctca aatggtgggc ggctatgcga gcgtgagaga tatccagaag caagccgcag
     2521 cccgtctcgg caacgattgc ctggatcgtc gccggctcga ctgccaggcg gaagactttg
     2581 ccatcccgcc cgcggtaggg cacattttca atgatcactc gctcgggacc gagctgatct
     2641 acggccaggc ggacatcctt gagcatgcgt gcaaacattt tgtcccgctc ggcaggtgaa
     2701 ggaagctcca gagagatgtc cgggaagtcc ttgctggttg cctccaggtg caggttgaca
     2761 tgcggggtac cggtatcgtc cagcatccgg gcgactgcac cccagtccag ggttttgatg
     2821 cggccattgc cggcttgcag accaaagtgc accgcgaccg ggcgataaac ccgcgccgct
     2881 gcgatcatat ccggccaatc catcgttttg aagcgatcga attcaatctt gccttgctcg
     2941 agcagcgcag ccgccggagt cgaataatta atggcgaggt ccatggtggt tatccgtggt
     3001 cagtcaatat taacgtatgc cggcgattcg tagatgatca agccggttag ggtctggtta
     3061 agggtgaata agagcaattt aatgagctta agggcaggcg tagtccagat ggccttggta
     3121 ggtctcagca aaattatgcc gcccggtatt gtcgcacttc gcccggaagt aataataagg
     3181 cgtctcggca ggataagcta cggcttccag ggctgccaga cccggattgg caattggacc
     3241 gggaggtaag ccgggattca ggtaagtatt gtagggtgag ttgatcgtca ggtcagcgct
     3301 gctgagcggg ttggtccacc aggttttctg gaccgggtta taacccgctg catattggac
     3361 tgtaggatcg gtgtccaact tcattccggc ggcgagccgg ttgtagaaca cggatgcgat
     3421 ggcaggcatt tcttcgtaca ggactgcctc gcgctccacg atcgaagcca gggttacagc
     3481 ctcatagagg ctgagcccct gcccggcgaa ggcttgctgg attgccggag tgacctgggc
     3541 ggcaaagttg accagaacca gggagatgca ttcttcagcg cccatgcggc gctcgattgt
     3601 gtaattacca gggaagagga atccttccag cgaagcgccg gcgggcagct cagccggcaa
     3661 actcaggtct gagggagggt gcttgacgat cctcaggaaa tcagccgggg tgaaccctaa
     3721 accactggca ggcagggcgg cggctatttc ctcgctgcgc cagccagcca ggatgctgag
     3781 ggtgacccgc gccggggtgg gatctttcaa ttcatgggca atttgcatgg cgctcatcgc
     3841 cgcgctcact tcatgctcgc cggcttgcag gatggtatcc aacccggcat aaaagaggaa
     3901 tgtgcgaaaa gcctccgggc tgctcaggta accctctgcc tgcaggcgat tcgagatcga
     3961 ggccaccgat tcgccgcttt caatcacaaa atagtgcatc tcatccgccg ggtcgcgcgg
     4021 ttgggttagc tggtcagcat tcgagagcag ctttgccgaa aggtaataca tctcggttga
     4081 atcc
//
LOCUS       DATMXK010000452         2449 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_86083, whole genome shotgun sequence.
ACCESSION   DATMXK010000452 DATMXK010000000
VERSION     DATMXK010000452.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2449)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2449)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2449
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_86083"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            116..1561
                     /locus_tag="VN363_08370"
     CDS             116..1561
                     /locus_tag="VN363_08370"
                     /EC_number="3.5.4.32"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016810 - hydrolase activity, acting on
                     carbon-nitrogen (but not peptide) bonds [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="8-oxoguanine deaminase"
                     /protein_id="HWQ84565.1"
                     /translation="MLSDNSARPDPASSTRDSILSTLLVRHATLLVTMDDQRREIADG
                     GLYIQDGFIAQVGSTASLPAEADEIIDLQDHLILPGLINTHHHFYQTLTRAVPAAQDA
                     NLFNWLKTLYPIWAGLTPPDIYLSTQTALAELALSGCTTASDHLYLFPNGSRLDDEIA
                     AGREIGVRLHASRGSMSLGESQGGLPPDRVVEDEAFIIKDSQRLIEAYHDPQPGAMTQ
                     IVLAPCSPFSVTGDLMRESASLARQFGVHLHTHLAETADEEVFCMEKFGYRPVGYMES
                     LNWTGEDVWFAHSVHVTSPEIEVYARTGCGVAHCPSSNMRLASGIAPVMDMLRAGVKV
                     GLGVDGSASNDSSNLLGEARMAMLAARVRAGLRGASLSSDAAPPLMSARQALEIATRG
                     SAAVLGRSDLGSLEPGKCADFFAVNLNRLDYAGALHDPVAAVVFCSPVRADLTVVNGK
                     TIVKGGHLQTLDLPHLIEKHNQAASRLLNQD"
     gene            1628..>2449
                     /locus_tag="VN363_08375"
     CDS             1628..>2449
                     /locus_tag="VN363_08375"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558554.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amidohydrolase family protein"
                     /protein_id="HWQ84566.1"
                     /translation="MKPAEIAAMTRGLVDVAMGRRPADLVIRSGRWISVQSGEIIPDT
                     DIAVLGERIAYVGPDASHTIAETTQIIQAEGRYISPGLLDAHMHVESGMLTLSEFVGA
                     VIPHGTTGIFIDPHEITNVFGLRGVRLMVDEAALQPIHVWVQMPSCVPSAPGLETPGS
                     RIGPEEVAEAMTWDGVIGLGEMMNFAGVYASDEIMHAEMAATRAAGKVIGGHYAAPDL
                     GLPFHGYVAGGPEDDHEGTRMEDAVARVRQGMKAMLRFGSAWHDVAAQIRAVTEMG"
BASE COUNT          495 a          711 c          689 g          554 t
ORIGIN      
        1 cctcaacccc gattaccgtc aaggaatcta taaaaattag agagaagttg tcggatattc
       61 tccaaatcgt atagttgtct gacaattgac acagctcacg ttctttgata cactggtgtt
      121 gtctgacaat tcagccagac ctgatcctgc tagttccacc agagatagta tcctgagcac
      181 actcttagtt cgccacgcga ccttgctcgt gactatggac gaccagcggc gtgaaatcgc
      241 cgacggaggt ctctatatcc aggatggatt catcgcccag gttggttcca ccgcttccct
      301 gccggcagag gcagatgaaa tcatcgatct gcaggatcat ctcattctcc ccggcttgat
      361 caacacccac catcattttt accagactct gacccgggct gttccggcgg ctcaagatgc
      421 caacctgttc aactggttaa aaacacttta cccgatctgg gctggtctca ccccaccgga
      481 catttatctc tctactcaaa ctgcactggc agagctggct ttatccgggt gtaccactgc
      541 ttcggatcac ctgtatctat ttcccaatgg cagccggctg gacgatgaaa ttgccgccgg
      601 gcgcgagata ggggtacgcc tgcatgcctc gcgaggctcg atgagcctgg gcgaaagtca
      661 ggggggtctg ccgcctgacc gtgtggttga ggatgaagcc ttcattatta aagattcaca
      721 gcgtctcatt gaagcttacc acgacccaca gcccggagcc atgacccaga ttgtgctggc
      781 tccctgctcg cctttcagcg taaccggcga cctgatgcgg gagagcgcca gcctggcgcg
      841 ccaatttggc gttcatttgc atactcatct ggcagagact gccgacgaag aagttttctg
      901 catggaaaaa tttggctacc gtccggtcgg ttatatggaa tcgcttaact ggaccggtga
      961 ggacgtctgg ttcgcccatt ccgtgcatgt cacttcccct gaaatcgagg tctacgcccg
     1021 cacaggctgc ggcgtggctc actgccccag ctcgaatatg cgcctggctt cgggtatcgc
     1081 cccggtgatg gatatgctgc gcgccggggt caaagttggt ctgggggtcg atggctccgc
     1141 cagcaacgac agctccaatt tgctgggcga agctcgcatg gcgatgctgg cggcgcgggt
     1201 gcgcgccggg ctgcgcggtg catcgctctc gagtgacgca gcgcctcctc tgatgtctgc
     1261 ccgccaggct ctggaaattg ccacccgcgg cagcgcggct gtcctcggtc gcagcgatct
     1321 gggctctttg gaacccggaa agtgcgccga tttctttgcc gtaaatttga accgcctgga
     1381 ttacgctggg gcgctgcatg atccggtcgc ggcagtggtt ttttgctccc cggtgcgcgc
     1441 tgatctcaca gtcgtaaatg ggaagaccat cgttaaaggc ggccatttac aaaccctgga
     1501 cctgccccac ttgattgaaa aacacaatca ggctgccagc cgtttactca accaggatta
     1561 aactcgaaac cggtactccc ttgaaaccat cgccaaccca accattcatc tgctcattgg
     1621 aggaggcatg aagccagccg agattgctgc tatgacgcgt ggcctggtag atgttgccat
     1681 gggccgccgg cctgccgatc tggtcattcg ctcgggtcgc tggatctccg tccaatcggg
     1741 agaaatcatc cccgatacag atattgctgt tctgggtgag cggatcgcct atgtaggccc
     1801 agatgccagc cataccatcg cagaaaccac ccagataatc caggccgaag gtcgttatat
     1861 ctccccgggt ttgctggatg cccatatgca tgtcgagtcc ggcatgctca ccctctccga
     1921 atttgtaggt gcggtgattc cccacggtac gaccggcata ttcatcgacc cgcacgagat
     1981 taccaacgta tttggcctgc gcggtgtgcg cctgatggta gatgaagcag ccctccagcc
     2041 catccatgtg tgggtgcaga tgccatcctg tgtaccctcc gcgcccggtc tggagactcc
     2101 cgggtccagg attggaccgg aggaagtcgc cgaggcgatg acctgggatg gtgtgatcgg
     2161 cctgggtgag atgatgaatt ttgcaggggt ctatgccagc gacgaaatta tgcacgccga
     2221 gatggcagct acccgggcag caggcaaggt gatcgggggc cattatgccg ccccagatct
     2281 cggtttacca tttcatggtt atgtggctgg cgggcctgaa gatgatcatg aaggcacccg
     2341 catggaagac gctgtcgccc gagtccgcca gggcatgaaa gccatgctgc gcttcggttc
     2401 tgcctggcat gatgtcgcag cccaaattcg ggctgtgacc gagatgggt
//
LOCUS       DATMXK010000453         2535 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_39527, whole genome shotgun sequence.
ACCESSION   DATMXK010000453 DATMXK010000000
VERSION     DATMXK010000453.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2535)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2535)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2535
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39527"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2519)
                     /locus_tag="VN363_08380"
     CDS             complement(<1..2519)
                     /locus_tag="VN363_08380"
                     /inference="COORDINATES: protein motif:HMM:NF012731.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003918 - DNA topoisomerase type II
                     (double strand cut, ATP-hydrolyzing) activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0006265 - DNA topological change [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA gyrase subunit A"
                     /protein_id="HWQ84567.1"
                     /translation="MRYTEARIASAAVDMLADIQKDTVNFSDNFDGTLTEPDVLPSAI
                     PNLLVNGATGIAVGMSTSIPPHNLGEVVDALTFMLGHWEKYEDITLDELMQFVQGPDF
                     PTGGIIIQDGDGDNLHSAYGTGRGKVTIQARAHIEEMERGRSRIIVTELPYLTNKSTL
                     IERIASLVRDERLEGIADLRDESDRQGMRIVIELNKAADPEKVLGNLYKSTPMQSTFS
                     IIVLALVEGEPRLLGLKQALKVYIEHRLDIIRRRSEFNLAQARARARILEGLLIALRN
                     LDEVINLIRRSRDADAAKENLIKKYKLSELQAQAILDMPLRRLAALERKKLEDEFKEL
                     QAQIKELESLLKSPRIMRQVVSDELSAVKEKHGDRRRTQIVTLKEGQTIRAVLTATDL
                     APEKAVWVSVTAQGLISRTLDEKPPVTKGREAPVLLAKCNTRHTLYLVNDLGEAGAVA
                     IQAVPEAVTPDEGVPFNRVTALKENDHLRAIFSLPTRAERSEDSFLLTVTQQGMVKKS
                     SLAELPGPAAHAFTLVKVNEGDSLGWLRITSGKSELLLLTENGMAIRFSEEEVRPMGL
                     VAGGVMGIKLAAGDVVSGMEILPHPGEVVLMTSDGSAKRITVDQFPIQGRYGQGVIAW
                     KLSSGVKTIGLVIGEMNERITLHLAKQAPKTMRVAEVPFATRAGRGKPVVDLKLDDEL
                     LRITITHDGETPLSKTTRKAGGGGSSGTEKPSKQAKPVVKPGGTVKDFRSAPKTASPG
                     TAKPTNKPATAKTMDNKAKAAAAVKSSPVTAKPDASLPQSARSRKPSSKTDSDQKATQ
                     LSLLDSAPPALKPSSKSAHEGKSAKSGSKTAAKKVTPDKKGA"
BASE COUNT          508 a          693 c          648 g          686 t
ORIGIN      
        1 gctccctttt tatcaggggt tactttttta gctgcggtct tactgccaga cttggcgctc
       61 ttaccttcat gggcagattt tgaggagggt ttcaaagccg ggggagcaga atctaacaag
      121 gatagctggg tagctttctg atcgctgtcg gttttagaag atggttttcg ggagcgggcg
      181 ctctgtggta aagaagcatc cggtttcgca gttacaggcg aggatttcac tgccgcggcg
      241 gctttggctt tgttgtccat cgtttttgct gtagctggct tgtttgtggg ttttgcggta
      301 cccggagagg ccgtttttgg tgcagacctg aaatctttga ctgtaccacc aggtttgacc
      361 acaggtttgg cttgtttaga tggtttctcc gttccactgc tgcctcctcc accagccttg
      421 cgtgtagttt tcgagagtgg agtttcacca tcgtgtgtaa tggttattct gagtagctcg
      481 tcgtccagct tcaaatccac gacaggtttg ccgcgccctg cccgggtcgc aaaggggact
      541 tcagcaactc gcatggtttt aggagcttgc tttgccaggt gtagagtgat ccgctcgttc
      601 atttcgccga tcactaatcc aatcgtttta actccggaac tgagcttcca ggcgatcaca
      661 ccctgaccat aacgcccctg gatcgggaac tggtcgacag ttatccgctt ggcgctgcca
      721 tcgctggtca tgagcacaac ttcgcccggg tggggcagta tctccattcc gctcaccaca
      781 tccccggcgg ctaatttgat gcccataacc ccaccagcta caagacccat tggtctaact
      841 tcctcttcag aaaagcggat tgccatccca ttttctgtaa gcagcagcag ctcgcttttg
      901 ccactcgtga tccttagcca acccagggaa tcgccttcat tgaccttgac cagggtaaaa
      961 gcatgcgcag caggcccagg caattcagcc agggaggact ttttaaccat cccctgctga
     1021 gtgactgtga gcaaaaagct gtcttcactt cgctctgcac gcgtgggcag ggaaaatatc
     1081 gctctgagat gatcattttc cttcaaggct gttactctgt tgaaggggac tccctcgtcc
     1141 ggggtcacgg cttcagggac agcctgtatg gcaacagcac cagcttctcc cagatcattc
     1201 accagataaa gtgtgtggcg ggtgttgcat ttcgccagta atactggtgc ttcccggcct
     1261 tttgtgacag gaggtttttc atccagggtc cgggagatca gtccctgagc tgttacagat
     1321 acccacacag ctttttctgg ggccaggtca gttgcggtaa gcacagccct tatcgtctga
     1381 ccctctttca gggtcacgat ttgagtcctt cgccggtccc catgcttttc tttgaccgcc
     1441 gaaagctcat cgcttacgac ctggcgcatt atcctgggtg atttcagcag gctctccagc
     1501 tctttaatct gtgcctggag ctctttaaac tcatcttcca gttttttgcg ttccagggct
     1561 gccaggcggc gtaagggcat atccaggatt gcctgcgctt gcagctcgct gagtttgtac
     1621 ttcttaatca aattttcttt cgccgcatcc gcatcccgcg agcggcggat taaattgatg
     1681 acctcatcca gatttcgaag ggcaattaac aggccttcca ggatacgggc gcgggcgcga
     1741 gcttgggcca ggttgaattc actccgccgc cggataatat ctagccggtg ctcgatatat
     1801 actttcagcg cctgcttcag gcccaacaaa cgcggttcac cttccaccaa ggccagcacg
     1861 atgatgctga aagtgctctg catgggagtg cttttataaa gattcccgag cactttttca
     1921 ggatcagccg ccttgttcaa ttcaattaca atccgcatac cctggcgatc agattcatct
     1981 cgcagatcgg cgatcccttc caggcgttca tcacgcacga gagatgcgat gcgctcgatc
     2041 aaggttgatt tgttggtcag atatggcagc tcggtcacaa tgatccggct gcgcccgcgt
     2101 tccatttctt cgatatgcgc ccgcgcctgg atcgtaactt tcccacgacc agttccataa
     2161 gccgaatgta agttatctcc atccccatct tgaatgatga tcccaccggt ggggaagtct
     2221 ggtccctgga cgaactgcat cagctcatcc agggtaatat cttcgtactt ctcccaatgc
     2281 ccgagcataa atgtcagcgc atcgacgact tcaccgagat tgtgcggtgg aatgctggtg
     2341 gacataccca cagctattcc ggtagcgccg ttgaccaata gatttgggat cgcggagggt
     2401 agtacatctg gctctgtcag cgtgccatca aaattatccg aaaaatttac cgtatctttt
     2461 tgaatatcgg cgagcatgtc tacagctgcg ctggcgatgc gcgcttcagt gtatcgcatg
     2521 gccgcaggtg ggtct
//
LOCUS       DATMXK010000454         7309 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_134063, whole genome shotgun sequence.
ACCESSION   DATMXK010000454 DATMXK010000000
VERSION     DATMXK010000454.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7309)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7309)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7309
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134063"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..518)
                     /locus_tag="VN363_08385"
     CDS             complement(<1..518)
                     /locus_tag="VN363_08385"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559098.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009897 - external side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0030975 - thiamine binding [Evidence IEA];
                     GO_function: GO:0030976 - thiamine pyrophosphate binding
                     [Evidence IEA];
                     GO_function: GO:0048502 - ABC-type thiamine transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015888 - thiamine transport [Evidence
                     IEA];
                     GO_process: GO:0030974 - thiamine pyrophosphate
                     transmembrane transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ84568.1"
                     /translation="MAWINKKSISIISILGVVLLASCQATPAATPTAMATAVPTSTAP
                     RQLSVMTHDSFAVSEEVVAAFESQHNARVQFIKGGDAGLALNKLILSKEQSLADVFYG
                     LDNTYLSRALAEGIFEPYASPLLAEIPAEFQLDPAQGALPVDYGDVCVNYDKAYLVEK
                     NLTPPQTLDDLLA"
     gene            complement(578..1237)
                     /locus_tag="VN363_08390"
     CDS             complement(578..1237)
                     /locus_tag="VN363_08390"
                     /EC_number="2.7.6.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01378.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004788 - thiamine diphosphokinase
                     activity [Evidence IEA];
                     GO_process: GO:0006772 - thiamine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="thiamine diphosphokinase"
                     /protein_id="HWQ84569.1"
                     /translation="MIIAGGWLSQLDQARDLIQPDDLLIAADGGAEHFRALELLPHVL
                     VGDLDSISSPTLAALEAAGVEILRYNPRKDYTDLELALHLAQERRCHPVLVFGAAGSR
                     WDQTIAGALLPAAGKFQDLDVRLIDERQELRLLNGGEALEVNGHPGDIVSLLALSETA
                     AGITTRGLEYPLNDEDLHLGATRGISNVLQGEHASIQLRKGLLLCIVLHGDPDSLGCP
                     G"
     regulatory      complement(1280..1390)
                     /regulatory_class="riboswitch"
                     /inference="COORDINATES: nucleotide
                     motif:Rfam:14.4:RF00059"
                     /inference="COORDINATES: profile:INFERNAL:1.1.4"
                     /note="TPP riboswitch; Derived by automated computational
                     analysis using gene prediction method: cmsearch."
                     /bound_moiety="thiamine pyrophosphate"
                     /db_xref="RFAM:RF00059"
     gene            complement(1444..2232)
                     /locus_tag="VN363_08395"
     CDS             complement(1444..2232)
                     /locus_tag="VN363_08395"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ84570.1"
                     /translation="MSAIILQDELVHYEVLGHGRPLVFLHGWVGSWRYWAATMQSVSG
                     TYRTYALDLWGFGTTSRQPANYSLVRQASLVEAFLEEMGIGKIALVGHGLGALVSLIL
                     AARIPRQVDRVMVVGLPGEYTALSPRLYTSQPQDLAEWLLARSLDGNAVRLETPKVDP
                     NAIRRSLEDLQAYNLPVLLETMTSPCLMVWGQNDLAVQAIPRERISGFPERIHGMTFE
                     ESGHFPMLDEPSKFQHLLIDFLTLDPGASLRQLQFREEWKRRVR"
     gene            complement(2252..2626)
                     /locus_tag="VN363_08400"
     CDS             complement(2252..2626)
                     /locus_tag="VN363_08400"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559090.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ84571.1"
                     /translation="MAKILIADDERDIRDLVAFALRFAGYEVVATSNGEDALRAAQTE
                     LPDLILLDVRMPRMTGYETCRLLKEQAFTRTIPVVFLSAKGQEAEVNAGMLLGAVEYI
                     VKPFSPDDLLKRVRAILANQPG"
     gene            complement(2611..6279)
                     /locus_tag="VN363_08405"
     CDS             complement(2611..6279)
                     /locus_tag="VN363_08405"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ84572.1"
                     /translation="MPISASLTVGLGLLIAGILLLALVVGLIDLARHKSQTAPRQAAH
                     KVEEAENEARITSDPPRPTEAVLIVKPGGSFGQINETARQWFQAEEAHPNLERLAQRA
                     YPAEAFLGLCAAPGEVQFTLDGRLVLGRSYEITQAGQRAVLVSLRQARNLLLGTEPSG
                     DPVLLMGGLTRKINANLELEPTLAAILECVEQIIPADLAQIGLWETGTQQLMTYRFFG
                     LPGTDRHLRKTLPGTWLGAGFTGRLVESRQVLRVDDLQADQGSPDLPSGENVNFRSYL
                     GAPLLDGDTLVGTLELIAHNPGYFQLEQAHFLLQVASLAAQALQNARHFEAERNNAAD
                     LKGLTELTQALQDVSDPDILFSRLADSVYALLDVEIMGFLLYDEGRRVLYGQNPFYGL
                     PPNVVNWTRLVIQPGSQAEELLSRAELIVSNQADQDSRLEALGVKAVAQAAGIRHLIL
                     APLASAGRLAGYLQAAQPRSGGGFDEAGIRRVQQIAAQVAPIVDNAFLVGEARRRVQR
                     SETFRRIASLTSSSATLDEVLKYSMLDLVRLLQADAGMILLVNTRRGELAPHLASVYG
                     IEVDYLTLLSQRLDEEADLSQLVTGSQQPLLAGNLAQESLHPIYHSLARELDLQSTII
                     VPLVTREQGVGELLIASHQPDLYDYSDERSVATAAGQLAGAIGLAEEHQRTAILLQII
                     TELSASLDLDQVLQRTLRVLNEFIRAQKVSLWLAPLEQPGLRRLATLDGDRLLVVETS
                     QEQDLARQVLKSGTYLRLDDLALGAPGPQDYGAVGSVLAVPLVIGAEILGVLEVFSDR
                     AGDFTSGHLELVLAAANQMAVAVNNAGLYNLIRDQAEGLGGMLRDQQIATSRSQAILE
                     AVADGVLVTDSQMKVTLFNASAEKILGLQRDRVLESSLDQFSGMFGKAAQTWMAAIQN
                     WSHDPLSAQTGESYAEKLDLENGRVVEVHLAPVSLRSDFLGTVSIIQDITHQVEVDRL
                     KSEFVATVSHELRTPMTSIKGYVEILLMGAAGSLSENQTHFLEVIHANTDRLIVLVND
                     LLNISKIESGRIELLPQPLDLDGLVSDALLIIEQRSAEDGKSIHLRKDIPLNLPQAWG
                     DVERVRQILENLLDNAYLYNQNGGSIQVHAGTQGREIQVDVVDTGSGIPPDEQGRVFE
                     RFYRGESPLILGVSGTGLGLSIVQSLVTMQGGRIWLQSQGIPGKGSTFSFTLPVYNGQ
                     QKSAHEENIEGISQWQRS"
     gene            complement(6281..7168)
                     /locus_tag="VN363_08410"
     CDS             complement(6281..7168)
                     /locus_tag="VN363_08410"
                     /inference="COORDINATES: protein motif:HMM:NF013180.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ84573.1"
                     /translation="MGKARLLIVEDDIDIANMLRIYFTSQNYSVDIAHRGGIALEKTR
                     QSLPNLIILDIMLPDIDGFEVCRNLRTHTRTSHVPIIFLTQKDERSDKLQGLELGADD
                     YVTKPFDIEELKLRVQRSIQRAEQQSLTDPRSGLPAGRLIKEQLRRTICQDNWAVMDI
                     RLNGFEPFKEVYGFLAADDVLRFVAMLLNEVLDASGTANDFLGHPGDDNFIVITTEPA
                     AQLVLKQLKARFEEEIQTHYNFIDRENRFITTTNTDGSANQHPLMTVAAGVVSPAQYQ
                     FADIREITEMAAEARRQAR"
BASE COUNT         1602 a         2161 c         2001 g         1545 t
ORIGIN      
        1 gccaggagat catccagggt ttggggcggg gtgagatttt tctctaccag gtaagcttta
       61 tcgtagttga cgcacacatc gccataatca accggcaaag caccctgagc cggatcgagt
      121 tgaaattcag ccgggatctc ggccagtaga ggcgaggcgt aaggctcaaa gatgccttct
      181 gccagggcgc ggctcagata ggtattgtcc aggccataga aaacatccgc cagagattgt
      241 tccttggaaa ggatcagctt attgagcgcc agcccggcgt cgcctccctt aatgaactgc
      301 acccgggcat tgtgctggct ttcaaaggct gccacgactt cctcggatac ggcaaacgag
      361 tcgtgggtca tcacactgag ctggcgaggc gccgtggatg tgggtacagc ggtcgccatc
      421 gcggttggcg ttgccgcggg tgttgcctgg cagctcgcca acaggacaac tccaagaatc
      481 gatataatcg agatggattt tttatttatc caagccatat tcttactcct atctccattg
      541 cgtactaata agcacgccac ccggctgcaa gcctaagcta cccgggacag cccagcgaat
      601 ccgggtcgcc atgcaggacg atgcataaca aaagcccttt gcgcagctgg atgctggcgt
      661 gttcgccctg taatacattg ctgatgcccc gggttgctcc caggtgcaaa tcttcatcgt
      721 tgagggggta ttccaatccc cgggtggtga tccctgccgc ggtttcactt aatgcgagca
      781 gtgagacgat atccccggga tgaccgttca cttccagggc ttctccgccg ttcagcaggc
      841 gcagctcctg gcgctcatcg atcaggcgta catccaggtc ctgaaattta cctgcagcgg
      901 ggagtaatgc cccggcgatg gtctggtccc agcgcgagcc ggccgcgccg aaaaccagta
      961 ccggatggca gcggcgttcc tgcgccaggt ggagggctaa ttccaggtcc gtgtaatcct
     1021 tgcgcggatt gtagcgtaaa atttccacac cggcagcttc cagcgctgcc agggtgggtg
     1081 aagaaatcga atccagatcg ccaaccagca catgcggcag caattccaga gcccggaagt
     1141 gctctgcccc accatctgcg gcgatcaaca gatcatcggg ttggattaag tctcgagctt
     1201 ggtccagctg gctgagccag ccgccggcga taatcacagc acgcaaaata aaaaccctcc
     1261 tgcgagcgga ggagggtggt gaatgtacct tgtgcgattc ctccgccggc attatccgga
     1321 tcaggttcgc gggtcaaggg ctcacagccc tcctctcagc ctggcaacac aggctccccg
     1381 tgcatcagca atgttagtta agattttatc gtgggcattg acaggtgtca agccgaaacc
     1441 agctcagcgg acgcgccgtt tccattcttc ccgaaattga agctggcgca agcttgcgcc
     1501 ggggtcgagg gttagaaaat cgatcaaaag gtgctggaat ttgctgggct catccaacat
     1561 cgggaaatgt cctgactcct caaaagtcat cccgtggatg cgttctggaa agccgctgat
     1621 acgctcccgg ggtatcgcct ggacagcaag atcgttctga ccccagacca tcaagcaggg
     1681 tgaagtcatg gtttccagaa gaacgggcag gttataggct tgcaggtctt ccagcgagcg
     1741 ccggatggca ttcggatcca cctttggcgt ttccaggcgg actgcgttcc catccaggct
     1801 gcgcgccagc agccattcgg ccagatcctg aggctgagac gtatacaggc gtgggctgag
     1861 ggcagtgtac tctccgggca agcccaccac catcacccgg tccacctggc ggggtatgcg
     1921 tgcggccaaa atcaagctga ccagggctcc gaggccatgc cctaccagag cgatcttccc
     1981 aattcccatc tcttccagga acgcctctac caggctcgcc tgccgtacaa ggctgtaatt
     2041 agccggctgc ctggaggtgg tgccaaaacc ccacaaatcc agggcatagg tgcgataggt
     2101 ccctgaaacg gactgcatcg ttgctgccca ataacgccac gaacccaccc aaccgtgcag
     2161 gaagaccagt gggcgaccgt gcccaaggac ttcgtaatgc acaagctcat cttgcaggat
     2221 aatcgcgctc attcgggttt gctgctcctg gctaccctgg ctggttcgct aaaatcgccc
     2281 ggacccgctt caacaggtcg tccggtgaaa acggtttaac aatatattcc accgctccca
     2341 acagcatacc ggcattcact tcggcttcct gtcccttcgc agatagaaat acaaccggta
     2401 tcgtccgggt aaacgcctgc tcttttagca gtcggcaggt ttcatagcct gtcatgcgcg
     2461 gcatccgtac atccagcagg atcaggtccg ggagctcagt ctgggctgca cgcagtgcat
     2521 cttcgccatt ggaagtcgcg accacctcat acccggcaaa gcgcagtgca aatgctacca
     2581 ggtcacggat atcgcgctca tcatcggcga tcaggatctt tgccattgtg aaattccttc
     2641 gatattttct tcatgggcag atttctgctg cccgttgtag actggcaagg tgaaagaaaa
     2701 ggtactgcct ttccccggga tcccctgact ttgcagccag atcctccccc cctgcatggt
     2761 caccagactc tggacaatgg agagacccag cccagtgcca gaaacgccca ggataagcgg
     2821 gctctcaccg cgataaaacc gctcaaatac gcggccctgc tcgtccggcg ggatgcctga
     2881 gccggtatca acaacatcca cctgaatttc acgtccctgg gtgccggcgt ggacctggat
     2941 actgccgccg ttctgattgt agaggtaggc attatccagc agattttcca gtatctggcg
     3001 cacgcgttcg acatcacccc aggcttgagg caggttcagg gggatatcct tgcgcaggtg
     3061 gatggattta ccatcctcag ccgagcgctg ctcgatgatc aggagagcat cactcaccag
     3121 tccatccaga tcaagcggct gaggcagcag ctcaatccgg ccggattcaa tcttcgagat
     3181 attcaggaga tcattgacca ggacgatcag gcgatccgta ttggcatgga taacttccag
     3241 gaaatgggtc tgattttcgc tgaggctgcc agccgcaccc atgagtaaga tctccacata
     3301 acccttaatc gaggtcatcg gcgtacgcag ttcgtggctg acagtggcga caaactcgga
     3361 ttttaagcgg tcaacctcaa cttgatgggt aatgtcttga atgattgaaa ccgtacccag
     3421 gaaatcactg cgcagggata ccggcgccag gtggacttca accacccgcc cattttcaag
     3481 atcgagcttt tcggcgtagc tttcgccagt ttgagcgctc aacggatcat gcgaccagtt
     3541 ctggatcgcc gccatccagg tctgggcagc tttaccaaac atgcccgaaa actgatcgag
     3601 ggagctttcc aggacccggt cacgctgcaa acccagaatc ttctcggctg aagcattaaa
     3661 gagggtgact ttcatctggc tgtcggtcac cagcacccca tcggcgaccg cttccaggat
     3721 cgcctgggag cggctggtgg cgatctgctg gtcgcgcagc atgcccccca ggccttccgc
     3781 ctgatctcgg atcaagttat acagcccggc attgttgacc gcgactgcca tctggttggc
     3841 ggcagccagc accagctcca gatggccgga ggtgaagtcg cctgcccggt cactgaaaac
     3901 ctccagcacc cccaaaatct ccgccccaat caccaatggg acggccagga cactgcccac
     3961 ggctccatag tcctgggggc caggcgcacc caacgcgagg tcatcgagac gtaaatacgt
     4021 gccgcttttc agcacttgcc gggctaaatc ctgctcctgg ctggtttcca caaccaggag
     4081 ccggtcgcca tccagggtcg ccagcctgcg cagcccgggc tgctcaaggg gtgccagcca
     4141 aagactcact ttttgagcgc ggataaattc gttcaacact ctgagcgtgc gctgcagcac
     4201 ctggtcgagg tccaggctgg ctgacaattc ggtgatgatc tgcagcagga tcgcggtgcg
     4261 ttgatgctcc tcagccagcc caatggctcc agccagctgc ccggctgcag tggccaccga
     4321 gcgctcgtcg ctgtagtcgt aaagatcagg ctggtggctg gcgatcaaca gctcacccac
     4381 tccctgctca cgcgtgacca ggggaacaat aatcgtggat tgcaaatcca gctctctcgc
     4441 cagggaatga tagatgggat gcaggctctc ctgggctaaa ttacctgcaa gcagaggctg
     4501 ctgtgagcca gtgaccagtt gagacaaatc tgcttcttcg tccaaccgct ggctgagcaa
     4561 agtcaggtag tcaacctcga ttccatacac cgaggccagg tggggggcta attcaccccg
     4621 tcgggtgttt accagcagga tcatgcccgc atcggcctgc agcagacgga ccaggtcgag
     4681 catcgaatac ttaagcacct catccagggt tgccgaggag ctggtcaggc tggctatccg
     4741 ccggaaggtt tcagaccgct gcacccggcg gcgcgcttcg cctaccagga aggcattgtc
     4801 aacgatcggt gctacctgag cggcaatttg ctgtacgcgg cggattcctg cctcatcaaa
     4861 tcccccaccg gagcgcggct gggccgcctg cagatagccg gccagcctcc cggcagatgc
     4921 cagcggggca aggatcaaat ggcggatacc ggcagcctgg gcaaccgcct ttacaccgag
     4981 cgcctccagg cgggaatcct ggtcagcttg gtttgaaaca atcagctccg cccggctgag
     5041 cagctcttcc gcctggctgc caggctggat caccagccgg gtccaattca cgacattggg
     5101 aggcagacca tagaaaggat tttgcccata cagcacacgc cgcccttcgt cgtagagcag
     5161 gaatcccatg atctctacat ccaggagagc atatacgctg tctgccaggc ggctgaagag
     5221 aatatccgga tcgctcacat cctgcagcgc ctgggtcagc tcggtgagcc ctttcagatc
     5281 ggcagcattg ttacgctcag cttcgaaatg acgggcattt tggagagcct gggctgccag
     5341 gctggctacc tgaagcagaa aatgagcctg ctcaagttga aaatatccgg gattgtgagc
     5401 aatcaattcg agcgtgccta ccagtgtatc tccatccaga agcggtgctc ccaggtagga
     5461 acggaaattt acattttcac cggatggaag atcaggtgaa ccctggtcgg cttgcagatc
     5521 gtccaccctg aggacctgac ggctctccac cagcctcccg gtaaaacctg ccccgagcca
     5581 ggtgcctggc agcgtttttc ggagatgccg gtcagtgcca ggcagtccaa aaaaacgata
     5641 ggtcattaat tgctgagttc cggtctccca gaggccgatt tgagccagat ctgcagggat
     5701 aatttgctcc acacattcca gaatggcggc taaagttggc tccagctcca ggttggcgtt
     5761 gatcttacgg gtaagcccac ccatgagcag gacaggatcg ccggatggtt ctgttcccag
     5821 aagcagattg cgagcctggc gcaggctaac cagtactgcc ctctggccgg cctgcgtgat
     5881 ttcataagac cttcccagca ccaggcgtcc atccagtgtg aactgcactt ccccaggagc
     5941 agcacataaa cccaggaatg cctccgctgg ataggcgcgc tgggccaatc gctccagatt
     6001 gggatgcgct tcttccgcct ggaaccactg gcgagcggtt tcattgatct gaccgaagct
     6061 gccgccgggt ttgactatca gcacagcttc cgtagggcgc ggtggatcac tggtaatcct
     6121 ggcctcattt tctgcttcct ccaccttatg agctgcctgc cgaggcgccg tctggctctt
     6181 gtggcgcgcc agatcgatca acccgaccac cagcgccagg agcaggatgc cagcaataag
     6241 cagaccaagc ccaacagtta aggaagcaga tattggcata ctaacgtgcc tgacggcgcg
     6301 cctctgccgc catctcggtt atctcacgaa tatcggcgaa ctggtactgg gcgggactga
     6361 ccaccccggc ggcaacagtc atcaaaggat gctggttggc gctgccatcg gtattcgtcg
     6421 tagtgatgaa gcggttttca cggtcgataa aattgtaatg ggtctgaatt tcctcctcga
     6481 aacgggcttt cagttgcttc aaaaccagct gagccgccgg ttcggtggtg atcacaataa
     6541 aattgtcatc gccgggatga ccgagaaaat cattcgccgt gccggatgca tctaatactt
     6601 cattcaacaa catcgctaca aagcgcagca catcatcggc tgccaggaat ccatagactt
     6661 ctttgaacgg ctcaaacccg ttgaggcgga tatccataac agcccaatta tcctggcaga
     6721 tggtccgccg cagctgctct ttaatcaagc gcccggcagg caatcccgag cgcgggtctg
     6781 tcaggctctg ctgctctgca cgctggatcg atctctggac acgcagcttc aattcttcaa
     6841 tatcgaacgg tttggtgaca taatcatcag cgcccagctc cagaccctgc agcttatcgc
     6901 tgcgttcatc tttctgggtc agaaagatga tcggcacatg gctggtgcgg gtgtgggtgc
     6961 gtagattacg gcagacttca aatccatcaa tatccgggag cataatgtcc aggataatca
     7021 ggttcggcag gctctggcga gttttttcca gagctattcc tcccctgtgg gcaatatcca
     7081 cgctgtaatt ctgggatgta aagtaaatcc gcagcatatt ggcgatatca atatcatctt
     7141 caacgattaa gagccgggct tttcccatac cagcctccca ttgatttaac ctgcctgaat
     7201 ttgcacagtt tgccttaatt caggtgacag tcttggactg cttgttcccg ggcggtccag
     7261 gagccaacca ggtggcatgc cgaaatctcg ccagagaccg cgtcgcttt
//
LOCUS       DATMXK010000455         2571 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_61763, whole genome shotgun sequence.
ACCESSION   DATMXK010000455 DATMXK010000000
VERSION     DATMXK010000455.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2571)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2571)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2571
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_61763"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            123..1202
                     /locus_tag="VN363_08415"
     CDS             123..1202
                     /locus_tag="VN363_08415"
                     /EC_number="2.3.3.10"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561252.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hydroxymethylglutaryl-CoA synthase"
                     /protein_id="HWQ84574.1"
                     /translation="MDDNNPIENLLLKPLRPVGIVGYGAYVPRYRLPATEVARVWTGG
                     QGALPIKEKAVPGIDEDVVTMSIEAARNALNRSAIDPGKIRAVWVGSESHPYAVKPTS
                     TLVAEAIGAVPNVQAADWEFACKAGTEAMVAAIGFVGSGMANYALAVGMDTAQGRPGD
                     ALEYTAGAGGAAFLLGPAEESLAVIDSTYSFVTDTPDFWRRQYQKYPEHGQRFTGEPA
                     YFKHVTEAASTLMSASHTTAADYRYAIFHQPNTKFPQRAGEILGFTKEQIAPGLLVPV
                     IGNTYAGAAIIGLTATLDIAEPGDRILMVSFGSGAGSDAFDIHVTEKLLERRSRAPLT
                     SDYIARRTLIDYAMYVRMRGKLTLK"
     gene            1242..2402
                     /locus_tag="VN363_08420"
     CDS             1242..2402
                     /locus_tag="VN363_08420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560135.1"
                     /note="Catalyzes the synthesis of acetoacetyl coenzyme A
                     from two molecules of acetyl coenzyme A. It can also act
                     as a thiolase, catalyzing the reverse reaction and
                     generating two-carbon units from the four-carbon product
                     of fatty acid oxidation; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="thiolase domain-containing protein"
                     /protein_id="HWQ84575.1"
                     /translation="MTDVVIAGIGQTSLGEHWEISLRELAFKAIEAALHDAQGLRPQA
                     LFVGNMLAPQLSRQAHLGVLISDFAGFNGIEALAVEAAGASGGAALRAGTLAVASGQA
                     DVALVVGVEKFTDKIGSDIDSALATAGDSDYESEQGLTPVAQAAMLMRRYMFQYDVPR
                     DGFAGFPINAHANGVGNPQAMFRKAIKPETYVQASLVSDPINLFDVAPNADGAAAVLL
                     TRADLLPPSFPNRLVRIAGASMVTDTLALHDRPDPLVFYAPGLSVERASQRARITPLQ
                     VDFFELDDAYSIYAALSLEAAGYAERGQGWKLAQDGQIGLHGSLPISTMGGSKARGNA
                     GGATGVYQAVEATLQLRGEAGPNQIPAARRALLQCLGGPATSAVTHVLESLN"
BASE COUNT          540 a          726 c          772 g          533 t
ORIGIN      
        1 aaacagtccg tatagatcgc gccggggaaa tattaacctc ctggcaatcg gataaaatta
       61 caaacccaga aaatcaaagt caatagccag tctggctttt tggcaccaag gattattaca
      121 gtatggatga taacaatccc attgaaaatt tactcctcaa acccctccgc ccggtaggga
      181 ttgtagggta tggggcgtat gttccccgct acagattacc ggcgacggaa gtggcacgag
      241 tctggaccgg cggacagggg gctctgccta tcaaggagaa agctgtcccg ggcatcgacg
      301 aggatgtggt gaccatgtcg atcgaagccg cccgcaatgc actcaatcgc agtgcaatcg
      361 acccaggcaa aatacgagcg gtgtgggtcg gctccgagtc gcatccgtat gcggtcaagc
      421 ccacttccac cctggtcgct gaggcgattg gagcggtccc gaatgtccag gcagctgact
      481 gggaatttgc ctgcaaggcg ggaacggaag cgatggtagc cgcaatcggc tttgtcggct
      541 ctggaatggc gaattacgcc ctggcggtgg gcatggatac cgcccagggt cggcccggag
      601 atgccctgga atacaccgcc ggagcgggtg gcgcagcttt tctgttgggt ccggcggaag
      661 aatcgctggc ggtgattgac tccacctatt cgtttgtgac cgataccccg gatttctggc
      721 gccgccaata tcagaaatac cctgagcacg ggcagcgctt caccggtgag ccagcctact
      781 tcaagcatgt cactgaagct gcctcaaccc tgatgagcgc cagccatacc accgcggctg
      841 attatcgcta cgcgatattt caccaaccca acactaagtt cccgcagcgg gcgggggaga
      901 tcctgggttt cacgaaagag cagatcgccc cgggtttgct ggtgccggtg attgggaata
      961 cttatgcggg ggccgcgatc atcggcctga cggctaccct ggatatcgct gagccaggtg
     1021 accggatctt gatggtttca ttcggctccg gagccggctc ggatgctttc gatatccatg
     1081 tcaccgagaa actgctggag cgccggtcgc gcgccccact gaccagcgat tatatcgccc
     1141 gccgtacact gatcgattac gccatgtatg tgcgcatgcg gggcaaattg actctgaaat
     1201 agaccggctg cccggccaga aattaaaaca ggtaaaccaa aatgactgat gttgtaattg
     1261 ccgggatcgg ccagaccagc ctgggtgaac actgggagat ttcgctgcgc gagttggctt
     1321 ttaaagccat tgaagcagcc ctgcacgacg ctcaaggact gcgcccacag gcactttttg
     1381 tgggcaatat gctcgcgccg cagctctcca ggcaggctca tctgggtgtg ctgatctccg
     1441 attttgcggg tttcaatggc attgaagcgc tcgcggtgga agccgccgga gcttcgggtg
     1501 gggcagccct gcgagccggg acgctggcgg tggcttcagg ccaggcggat gtggcactgg
     1561 tcgtaggggt ggaaaaattt accgataaaa tcggttccga cattgattct gccctggcga
     1621 ctgccggaga cagcgactat gaatctgagc aaggcctgac cccggtagcc caggctgcca
     1681 tgctgatgcg ccgctatatg ttccagtacg atgtgccccg cgatggtttt gccggttttc
     1741 cgatcaacgc ccatgccaat ggcgttggca acccccaggc tatgttccgc aaggcgatca
     1801 agccggagac ctatgtccaa gccagcctgg tcagtgatcc gattaatctt tttgacgtgg
     1861 cgcccaatgc cgatggggcg gctgcagtcc tgctcacgcg agccgatctg cttcccccca
     1921 gcttcccgaa tcgattggtg cgcatcgcag gtgccagcat ggtcaccgat accctggctc
     1981 tccacgaccg gcctgatcca ctcgtgtttt acgcgccagg cctgtcggtc gagcgcgcct
     2041 cccagcgggc caggattacc ccgctccagg tggatttttt cgagctggat gatgcttatt
     2101 cgatctatgc ggctttgtcg ctggaagcgg caggctatgc cgagcgtgga cagggttgga
     2161 agctggccca ggacgggcag atcggcctgc acggctcgct gccaatcagc accatgggcg
     2221 gctcgaaggc gcgcggcaat gccggcgggg cgaccggtgt ataccaggcg gtggaggcaa
     2281 ccttacagct gcggggggaa gccgggccaa accagatacc cgctgcccgg cgcgccctgc
     2341 tgcagtgtct gggtgggcca gcgacctcgg ccgttaccca tgttctggag agcctgaatt
     2401 agtctattta tgtgcaaaat ggcacgaagc gggcaaattg atagttcctg agagtcttta
     2461 acgactaaac tgctatcgtt cgctgagaac aattggtgaa accaatcgtt aaggagctat
     2521 aaattatgga aattccacgc cattggcgtc ttcgcaaaca acgctacagc c
//
LOCUS       DATMXK010000456         2078 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_59089, whole genome shotgun sequence.
ACCESSION   DATMXK010000456 DATMXK010000000
VERSION     DATMXK010000456.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2078)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2078)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2078
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_59089"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..289
                     /locus_tag="VN363_08425"
     CDS             <1..289
                     /locus_tag="VN363_08425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84576.1"
                     /translation="HGSDNSADFPPPPGVVLVPPVFVHPTAQIQNSIVGPNVSIGADC
                     IVENSIISDSILEDDAQTNRVILEASLIGRSAHISRRAGIINAGDNSVVTL"
     gene            330..1493
                     /locus_tag="VN363_08430"
     CDS             330..1493
                     /locus_tag="VN363_08430"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558744.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030170 - pyridoxal phosphate binding
                     [Evidence IEA];
                     GO_process: GO:0009058 - biosynthetic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class I/II-fold pyridoxal
                     phosphate-dependent enzyme"
                     /protein_id="HWQ84577.1"
                     /translation="MKSSYLAQHIVTLKPSGIRKFFDIAATMDDVISLGIGEPDFVTP
                     APIINAGIRSLQAGETHYTSNHGLIELRRAVSDYLQRNYEVTYDPANEIVITVGVSEA
                     LYLSMTAILNPGEEVIIPTPCFVAYQAEVVLAGGVPIELPTYAADGFMPRREAIEAAI
                     TPRTKAILIGYPNNPTGAVATRQAMQEIAEVAAAHDLVVISDELYDQLVYGVEHVCFP
                     ALSGMRPRTLLLGGFSKNYAMTGWRIGFAAGPVELIEGLVRIHQYTIMSAPTPAQAAA
                     LEALRSGAPHVEEMRQEYDRRRKLVVSGLNQLGLSTFEPHGAFYAFPNIRASGMDDDT
                     FANRLLAEERVAMIPGTAFGPGGENFVRCSYATSYDRLEEALHRIERFMRRYG"
     gene            1508..>2078
                     /locus_tag="VN363_08435"
     CDS             1508..>2078
                     /locus_tag="VN363_08435"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560850.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     GatCAB subunit B"
                     /protein_id="HWQ84578.1"
                     /translation="MEYEAVIGLEVHAELQTRTKMFCACPVVDPTTALPNTAVCPVCA
                     GLPGVLPVINAQAVRYALKVALALQCEILPASIFARKNYFYPDLPKGYQISQYEQPLA
                     VNGLLEFRTPQGLRSVRIRRVHIEEDTGKLTHLGDLAGENPANAPRSLVDLNRAGIPL
                     LEIVSEPDLHNAEEVRLYAMALRSLLRYLD"
BASE COUNT          395 a          682 c          545 g          456 t
ORIGIN      
        1 acacggttca gataacagcg ccgatttccc gccacctccc ggagttgtcc tggtcccgcc
       61 ggttttcgtc caccccactg cccagatcca gaactcaatc gttggcccca atgtttccat
      121 cggcgccgac tgtatcgttg aaaacagcat catcagcgat tcgattctgg aggacgacgc
      181 ccagaccaac cgtgtgatcc tcgaggcctc cttgattgga cgctctgccc atatctcccg
      241 ccgcgccggg atcatcaatg ccggcgataa ctcggttgtc accctttaag tccgcatatt
      301 tagcgtgact ctactctcaa ggagccagtg tgaaatcttc ctacctcgcc cagcacatcg
      361 ttactctgaa accttccggg atccgaaaat ttttcgacat cgccgccacc atggatgatg
      421 taatctcgct cgggattggc gagcccgatt tcgtcacccc tgcccccatt atcaatgctg
      481 gcatccgctc gttgcaggct ggcgaaaccc actacacctc caatcacggc ctgattgaac
      541 tgcgccgcgc cgtgtctgat taccttcagc gcaattatga agtcacatac gaccctgcca
      601 acgagatcgt gatcaccgta ggggtttctg aagccctgta tctttccatg actgctattc
      661 tcaaccccgg cgaggaagtg atcattccta ccccctgctt tgtagcctac caggcagagg
      721 ttgtcctggc tggcggtgtg cccattgagc ttcccaccta tgccgcggat ggctttatgc
      781 cgcgccggga ggccattgaa gctgccatca ccccccgcac caaagcaatc ctgatcggct
      841 atcccaacaa tcccaccggc gcagttgcca cccgccaggc catgcaggaa atcgctgaag
      901 tcgccgccgc tcacgatctg gtcgtcattt cggatgagtt atacgaccag ctggtatacg
      961 gcgttgagca tgtctgcttt ccggctctgt cgggtatgcg cccgcgcacc ctcttgttgg
     1021 gcggtttttc caagaattat gcgatgaccg gctggcggat cggctttgct gccggcccgg
     1081 ttgagctgat tgaaggtctg gtgcgtatcc accagtacac catcatgtct gctccgactc
     1141 ctgcccaggc agccgcactg gaagctctgc gcagcggcgc cccgcatgtg gaagagatgc
     1201 gccaggagta cgatcgccgc cgcaagctgg tcgtcagcgg gttaaaccag ctgggactgt
     1261 ccacgttcga gccgcatggc gctttctacg ccttccccaa tatccgcgcc tctggaatgg
     1321 atgacgatac gttcgccaac aggcttctcg ctgaggagcg agtggcaatg attcccggaa
     1381 cagcgtttgg ccccggcgga gaaaatttcg tccgctgctc atatgccacc agctatgaca
     1441 gattggaaga agccctccac cgcatcgaac gcttcatgcg ccgctacggt taatctttac
     1501 ctccggcatg gaatacgaag ctgtcattgg tctggaggtt cacgccgagc tgcagacgcg
     1561 caccaagatg ttctgtgcct gcccggtcgt tgacccaacc acagccctgc ccaacactgc
     1621 ggtctgcccg gtgtgcgccg gtctgcccgg ggtgctgccg gtgatcaatg cccaggcggt
     1681 gcgctatgcg ctcaaggtcg ctctggccct gcagtgcgag attctccctg ccagtatttt
     1741 cgcccgtaaa aactattttt accccgatct ccccaaaggc taccagatct cccaatacga
     1801 gcagcccctg gcagtcaatg gccttctgga gtttcgcacc ccccaggggc tgcgcagcgt
     1861 gcgcatccgc cgggttcata tcgaggagga taccggcaag ctgactcatc tgggtgacct
     1921 ggctggagaa aatcccgcca acgcgccgcg ctcgctggtc gacctcaacc gggctggcat
     1981 acccctgctg gagattgtct ccgagcccga tctgcacaac gccgaagaag tgcgcctgta
     2041 tgccatggcg ctgcgctcgc tgctgcgcta cctggatg
//
LOCUS       DATMXK010000457         2269 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_130289, whole genome shotgun sequence.
ACCESSION   DATMXK010000457 DATMXK010000000
VERSION     DATMXK010000457.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2269)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2269)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2269
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130289"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..500)
                     /gene="recO"
                     /locus_tag="VN363_08440"
     CDS             complement(<1..500)
                     /gene="recO"
                     /locus_tag="VN363_08440"
                     /inference="COORDINATES: protein motif:HMM:TIGR00613.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RecO"
                     /protein_id="HWQ84579.1"
                     /translation="MPGRERSQRVEAVVLRHNDWGEADRILVLYTLEMGKIRVVAKGV
                     RKLRSRKAGHLEPFTKAALQLARGRDMQIVTQADTLDAYLGLRDDFLKTTYAAHVVEL
                     LDRFTFEEGENPAVYRLLVKTLERLSADLPPEITVHYYRIRLLDLLGFRPRLFQCSVC
                     AKDIQP"
     gene            654..1166
                     /gene="rplI"
                     /locus_tag="VN363_08445"
     CDS             654..1166
                     /gene="rplI"
                     /locus_tag="VN363_08445"
                     /inference="COORDINATES: protein motif:HMM:TIGR00158.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000311 - plastid large ribosomal subunit
                     [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L9"
                     /protein_id="HWQ84580.1"
                     /translation="MKVLLLKDVYKLGRAGDVKRVADGYGRNYLIPQGLAVLATPGAL
                     KQAEQIRTTAATHRSALNKELGGVAQQLGKLILTFPAKAGETGKLYGSITPQMIADVI
                     REKSGVEVTRRQLDFEPIRTLGEHKIHIRLTMDLIPEVVVIVHREGEAVAIAQEAEPE
                     IPETAAEAEA"
     gene            1295..>2269
                     /locus_tag="VN363_08450"
     CDS             1295..>2269
                     /locus_tag="VN363_08450"
                     /inference="COORDINATES: protein motif:HMM:NF024252.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWQ84581.1"
                     /translation="MNEQIGQQLRQTREAQHISQAQAARDTLIRIHYLQALEAGDIQK
                     IPSSTQARGFLRAYSEYLGLDAEALIGSLNGTSQKTEPIEENTPAQEASPAPSGSQAD
                     EIFQRIGQKLKYQRSQLGLTLEDVERHTHLRQRYLTALENGDLNNLPSPVQGRGMLKN
                     YASFLGLDPDPVLMQFAEALQARLVVNQAATRANSPTRPREPARPARSLPGPLQRLFS
                     GDLLAGALLSIVLISFIIWGAARIFTMQSAGSASPTVPSIAEVFLTTPSATASPEPPS
                     TTPTVQSGVLPGFIAGSPTAETPGSIALPASQERVQVYVSVYQRAYMQV"
BASE COUNT          530 a          664 c          579 g          496 t
ORIGIN      
        1 cagggctgaa tgtctttcgc acagactgag cattgaaaca gccgggggcg aaatcccagt
       61 aaatccagga gccggatgcg gtagtaatgg acggttattt ccggtggcaa gtcagcactc
      121 aggcgctcaa gggtttttac gagcaggcgg tatactgccg gattctcacc ctcctcaaag
      181 gtaaagcggt cgagaagctc cacaacatgc gcagcatatg tcgtcttcaa gaagtcatcc
      241 cgtaagccca gataggcatc cagggtatcg gcctgagtga caatttgcat atcgcggccg
      301 cgggcgagtt gaagggcggc ttttgtgaat ggttctaaat ggccggcctt gcgcgagcga
      361 agcttacgga cacctttggc gaccacccgg attttaccca tctccagagt gtacagcacc
      421 aggatccgat ctgcttcgcc ccaatcatta tggcgtaaaa cgaccgcttc tactcgttgg
      481 gatcgctctc ttccaggcat gtctggatta tatcagcaag caaatgaatg gctggttgac
      541 tcccggggct gcctctggta aaatccccct gcccaaagcg ttgtaccctt cgctttgggc
      601 caatctgcgc gtagcgcgat tgaattcctc attttcgtcg aaagggcttg agcatgaaag
      661 tactcttact caaggacgtt tataaattgg gtcgggcagg agatgttaaa cgcgtagccg
      721 atggttacgg gcgcaactac ctgatccccc agggcctggc ggtgttagct actccaggcg
      781 cgttgaagca agccgagcaa atccgcacca ctgcagccac tcaccgttct gcactcaata
      841 aggagctggg aggtgttgcc caacaactcg gcaaactaat ccttacattc ccagccaaag
      901 ccggtgaaac cggcaagctt tatggatcga tcactcccca gatgatcgcc gatgtgatta
      961 gagaaaaatc tggcgtcgaa gtcacccgcc ggcagttaga ttttgaacct atccgcacat
     1021 taggggagca caagatccat atccgcctga ccatggattt gatccccgag gtcgtggtca
     1081 tcgttcaccg cgagggtgaa gctgtcgcca tagcacaaga agcagagcct gagattcctg
     1141 agactgccgc agaggcagag gcttaaccgg agacaaccgg ctgcaatttc ttgctgattg
     1201 gcatcgattt tagcccacca accggcctgg acggggcagc ggtaagcgag atcccttact
     1261 gcaacaccga ccaggccggt gtccttttct tagcatgaac gaacaaatcg gtcaacaact
     1321 ccgccagact cgcgaggcgc agcatatttc tcaggcacag gccgcccgag atacgctgat
     1381 ccgcatccat tatctgcaag ccctcgaagc tggcgatatt caaaagatcc cttcttcaac
     1441 ccaggcgcgg ggatttttgc gcgcttattc tgaatatctg gggttggatg cagaagcgct
     1501 gatcggctct ttgaatggca cctctcaaaa aaccgagcca attgaagaaa acacacctgc
     1561 gcaagaagcc tcaccggcac ccagcggctc acaggcagat gaaattttcc aaagaatcgg
     1621 tcaaaagctg aaatatcagc gcagccagct tggccttacc ctcgaagatg ttgaacgcca
     1681 tacacacctc cgccaacgtt atttgacagc cctggaaaat ggcgacctga ataatctacc
     1741 ttcccccgtc cagggccgcg gtatgcttaa gaattatgcc agctttctcg gcctggatcc
     1801 tgacccggtg cttatgcaat ttgctgaggc acttcaagcc cgtctggtag ttaatcaagc
     1861 tgccactcgg gcaaactctc ccaccaggcc cagagagcca gcccgccctg cccgctcgct
     1921 gccgggtccc ctgcagcgat tgttttcagg agatttgctg gcaggtgctt tattgagtat
     1981 cgttcttatc agctttatca tctggggcgc agcccgcatt tttaccatgc agtctgcagg
     2041 ctccgcctct cctactgtac catccattgc tgaagttttc ctgactaccc ccagcgcaac
     2101 cgcttcacct gaaccgccct ccaccactcc caccgtacaa tcaggcgttc tgccaggctt
     2161 catcgccggc agcccgaccg cagagacgcc gggcagtatc gcactccccg ccagccagga
     2221 gcgggtgcag gtatatgtca gtgtatacca gcgggcatat atgcaggtg
//
LOCUS       DATMXK010000458         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_118518, whole genome shotgun sequence.
ACCESSION   DATMXK010000458 DATMXK010000000
VERSION     DATMXK010000458.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_118518"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VN363_08455"
     CDS             <1..621
                     /locus_tag="VN363_08455"
                     /inference="COORDINATES: protein motif:HMM:NF021926.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="FIST C-terminal domain-containing protein"
                     /protein_id="HWQ84582.1"
                     /translation="GVALAGNLDIDILVSQGCRPIWRPFVVQSANKNWIYQLERKVPL
                     AWIQDLIPELSEEEQALLQNGLFVGRAVKSGQEIFGQGDFLIRGVMGVDQHTGAIAVA
                     DQIQEGEVIQFHIRDAVTAAEDLEMMLIPQMFSKPPDGALLFTCNGRGTRLYDHPDGD
                     ISVIRKNLGDIHLAGFFCAGEIGPIGDQNFIHGHTASLVLFRSPDI"
     gene            complement(812..1447)
                     /locus_tag="VN363_08460"
     CDS             complement(812..1447)
                     /locus_tag="VN363_08460"
                     /EC_number="2.7.7.87"
                     /inference="COORDINATES: protein motif:HMM:TIGR00057.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0061710 - L-threonylcarbamoyladenylate
                     synthase [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="L-threonylcarbamoyladenylate synthase"
                     /protein_id="HWQ84583.1"
                     /translation="MAQTVLISALTTETITAAVKQALQVLERGGLVAFPTDTVYGLAA
                     SAFDTHGIDRLFQAKERQIEKAIPILLASPADLEQVTLDMPAHARILAQRFWPGPLTL
                     VVPRHPALPENISPSSTIGVRMPDHPVALAILRASGPLAVTSANLSGFPSSTNASQVF
                     NQLDGRVDLIIDGGNTSGGIPSTVVDCTGPAPKVLREGPLSKTDLDQALQV"
     gene            complement(1462..2193)
                     /locus_tag="VN363_08465"
     CDS             complement(1462..2193)
                     /locus_tag="VN363_08465"
                     /inference="COORDINATES: protein motif:HMM:NF012888.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008146 - sulfotransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sulfotransferase domain-containing protein"
                     /protein_id="HWQ84584.1"
                     /translation="MIILSIGMPRAGSGWYYNLMHDLVTASGGQNARQIRSQFHLQSI
                     LTEVNCNIGALSLRRLSAVMIPSTLGKTFVIKAHAGPTPFARRLIDHRQMKAAYIYRD
                     PRDALLSAYDNGQRAIQKGRPNAFSHLVDFDAALEFMREYVRISEAWLNLPGVLHTSY
                     EDLVENYDLEATRLAKFLECSLESADIQAVIEQYRPGQGRQDQKGLHFNKGVRGRFRE
                     KLTPDQQATLANAFGGYLQRLSYPI"
BASE COUNT          540 a          556 c          635 g          531 t
ORIGIN      
        1 ggggtggcgc tggcaggaaa tctggacatt gatatcctgg tttctcaggg ctgccggccg
       61 atctggcgac catttgtcgt ccaatccgcc aataaaaatt ggatttacca gctggagcgt
      121 aaggtcccgc tggcctggat ccaggattta atccctgaat tatccgagga agaacaggct
      181 ttgctgcaaa acggcttatt tgttggccgg gctgtaaaga gcgggcagga gattttcggg
      241 caaggagatt tcttgatccg gggtgtgatg ggcgtcgatc aacacactgg tgcaatagcc
      301 gttgccgacc agatccagga aggtgaagtg atccaattcc atatccgaga tgcagttacg
      361 gctgcagaag atctggaaat gatgctaatt ccgcagatgt tttcaaaacc ccctgacggt
      421 gcgcttttat ttacctgtaa tggccgcggc acccgcttat acgatcaccc cgatggggat
      481 atatcggtaa tcagaaaaaa cctgggcgat atccacctgg caggcttttt ctgcgccgga
      541 gaaattggcc cgatcgggga ccagaatttt attcacggac atacagccag cctcgtgctc
      601 tttcgctctc ctgacattta aactcaaact gcgcatcagg tcggctgcca actttcctgg
      661 atgcgcagta ttgagtgagg agaatgcaac tgttattata cagtttgact tgagaggggc
      721 atgaaataaa gctgaacatt caatgagaac agaccggaag tatatcaaaa accctcccgg
      781 gctgtttttg attggtgacg ctgtcttctc gttaaacctg caacgcctga tctagatcgg
      841 ttttggagag gggaccttcc cgtaaaacct tcggagctgg acctgtgcag tccacgaccg
      901 tggaagggat gcctccgctg gtatttcccc catcgatgat taaatctacc cgaccgtcaa
      961 gctggttaaa gacctggctg gcgttcgtcg agctggggaa gccagacagg ttggcggaag
     1021 taaccgccag gggaccactt gctcgcaaga tagccagcgc aaccgggtgg tctggcatgc
     1081 gcacaccgat agtcgacgag ggggaaatat tttctggcag agccgggtgg cgtgggacga
     1141 ccagagtcag cggcccaggc cagaagcgct gcgcaaggat acgagcgtgt gctggcatgt
     1201 ccagcgtgac ttgttccagg tctgcgggac tggcgagtaa tattggaata gctttttcga
     1261 tttgacgttc tttagcctgg aacagccggt cgatcccatg tgtgtcgaag gctgaagctg
     1321 ccagtccata gacggtatcg gtcgggaagg caaccagacc accgcgttcc agcacttgca
     1381 gggcctgctt gaccgccgct gtgattgttt ctgtagtcag tgcggagatg agcacagtct
     1441 gtgccatcaa ttctccatac tctaaattgg atagctcaaa cgttggagat aacctccaaa
     1501 agcatttgcc agggtcgctt gctgatcggg tgtcagcttt tcacggaagc gaccgcgcac
     1561 acctttattg aagtgcaggc ctttttgatc ctggcgaccc tgcccggggc gatactgctc
     1621 gattacagcc tggatatcgg ctgattcaag cgagcattcc aggaatttcg ccagtcgagt
     1681 ggcttcaaga tcgtaatttt ctaccagatc ctcataagag gtatgcaaga ctccgggcag
     1741 attcaaccag gcttcggata tccgcacata ttcgcgcata aattccaatg ccgcgtcaaa
     1801 atccaccagg tgggaaaatg cattgggacg gcctttttga atggcacgtt ggccattgtc
     1861 atatgcggaa agcagagcat cccgtgggtc gcggtagata tatgcagcct tcatctgccg
     1921 gtggtcgatt aatcgccggg caaagggagt aggtccggca tgggctttga tgacaaaagt
     1981 tttccccagg gtcgagggaa tcatgactgc cgaaaggcga cgcaggctca gtgctccaat
     2041 attgcaattg acttccgtga ggatgctctg caaatgaaat tgggaacgaa tctgacgggc
     2101 attctggccg ccgctggcag tgaccagatc gtgcatcaaa ttgtaatacc agcccgaacc
     2161 ggctctgggc atgccaatcg aaaggatgat catagaaaag cgacactcct ggcccgccat
     2221 gttttcaggc gagtaagtaa gttgtaccga taattataca gg
//
LOCUS       DATMXK010000459         2011 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_162996, whole genome shotgun sequence.
ACCESSION   DATMXK010000459 DATMXK010000000
VERSION     DATMXK010000459.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2011)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2011)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2011
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_162996"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            288..629
                     /locus_tag="VN363_08470"
     CDS             288..629
                     /locus_tag="VN363_08470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84585.1"
                     /translation="MLNPYLIILIMAAAGFVLGLITFIVGIVILLFRPSNSDVKALIA
                     QSTALAQKGIAEDVAGLVGHVSTLLESLNQLVKTTSGVGILLVILGVLLMAGSSWLAL
                     QVSKVSGLTLE"
     gene            685..>2011
                     /locus_tag="VN363_08475"
     CDS             685..>2011
                     /locus_tag="VN363_08475"
                     /inference="COORDINATES: protein motif:HMM:NF025910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ84586.1"
                     /translation="MATEPTPQSTHWLLSLAKHFDHRVWPWLIRALRHDPIVWEALEK
                     HGMAERGLAALSGDPKKWSPAVLALMSLDLSAPLESLRSLPLRPVSQAVHERALKTQE
                     KWVESAGSGSLNLGQAVLIALALREDRLGSSWDQVFASLDLSHPGWQTIISCLYGIIP
                     DPFDMLRAFMQPGDKTFQPQIVVHTLLSNPLSPDEQGSALRDLLKALPADQRKAALQI
                     LSQVRKQLAVEIAQDVLDDRLNETSTPHSLAVSKSLPTKNTWSTRLAGLQDTLDNADF
                     YITAGQEAQALPMISEAITSTRRIQAGLSAYLAELVSTIGNPESAVEAWRQAAQMEPE
                     NPLNTAGLVQSLLDMKRVEDARTILDLHLADLDAAGMHPVLVLAAAQLAVTEGHLQDA
                     RNTAEQVFAQLDGSPELKTSKNLAALARTLLAAGLPNLAELAAGRGIQND"
BASE COUNT          441 a          597 c          545 g          428 t
ORIGIN      
        1 gctgctgggc aatcattctc gcaccgatct tgagatcgag cacttcattc aagaatggca
       61 gatgccgctg atcctggctg gacgcccgtc ctgtgagttc cctactctgc aggccagcca
      121 gatccagatc ggcgctcaat aagcatctgt attcgaacct cctctcctat cttattggtc
      181 gaaacccaca ggcccggcaa aaaccgggtc tgtgagttta acagccgcat tggaacgtct
      241 ggcattcttc ttgcaacatc aggtgcatac tagcgaggag agtgggcatg ttgaatccat
      301 atttaattat cctgatcatg gcagccgccg gttttgttct tggcttgatc accttcattg
      361 tgggcatcgt cattctcctt tttcgtccct caaactcgga tgtcaaggct ttgatcgccc
      421 agagcaccgc cctggcccag aaagggatcg ctgaagatgt cgccggtctg gtcgggcatg
      481 tctcgaccct gctggaatcg ctcaaccagc tggtcaaaac gacttcaggc gtgggtatct
      541 tgctcgtcat actgggggta ctgctgatgg ccggctcttc ctggctggca ctccaggtct
      601 ccaaggttag tgggctcacc ctcgagtaat ccactccagc cggcgcttat cgggtaataa
      661 cgaaccaata caggcagaat aaccatggca actgaaccaa ccccacagag tacacactgg
      721 ctcctgagct tagcaaaaca ctttgatcac cgcgtctggc cgtggctgat ccgggccttg
      781 cgccacgatc ccattgtatg ggaagcgctg gagaaacacg gtatggcgga gcgtgggctg
      841 gctgcactgt ccggcgatcc gaaaaaatgg tcgccagcgg tgctggcttt aatgagcctg
      901 gatctttctg ccccactgga aagcctgcgt tcactgcccc tgcggcctgt atcgcaagca
      961 gttcacgagc gggcgctaaa aacccaggaa aaatgggttg aaagcgccgg ttcaggttca
     1021 ctcaacctcg gacaggcggt tttaattgcc ctggctctgc gcgaagatcg cctgggctct
     1081 tcctgggatc aagtctttgc gagcctggat ctctcccacc caggatggca gactatcatc
     1141 agctgcctgt atggaattat ccccgaccca tttgacatgc tgcgggcatt catgcagccc
     1201 ggggataaga cgttccaacc ccagatcgtg gtgcatactt tgctcagcaa cccacttagc
     1261 ccggacgaac agggttccgc actgcgggac ctcttgaaag ctctgccggc cgatcaacgc
     1321 aaggcagcct tacaaatatt gagccaggtt cgcaagcagc tggcggttga gatcgcccag
     1381 gacgtgctcg acgacaggct caatgaaaca agcactcccc acagcctggc ggtgagcaaa
     1441 tctctgccca caaagaatac ctggtccaca cgtctggcag gtctgcaaga caccctggat
     1501 aacgccgatt tctatatcac tgccgggcaa gaagcccagg cactgcccat gatttctgaa
     1561 gcgatcacca gcacgcggcg catccaggcc gggctgtctg cttatctggc tgagctggtc
     1621 agcacgatcg gcaatccaga aagtgcagtt gaagcctggc ggcaggctgc ccagatggag
     1681 cccgaaaacc cgcttaatac tgccgggttg gtccaatccc tgctggatat gaagcgggta
     1741 gaagatgccc ggaccatcct ggacctgcac ctggccgacc tggatgcggc tggcatgcat
     1801 cctgttctgg tgctggcagc tgcacagctg gctgtaactg aagggcacct gcaggacgcg
     1861 cgcaatactg ccgagcaggt tttcgcccag ctggatggct cgccagagct gaaaaccagt
     1921 aaaaatctcg cagcgcttgc cagaacactc ctcgcggcag ggctgcccaa tctagccgaa
     1981 ctagctgccg ggcgcggtat acaaaatgac c
//
LOCUS       DATMXK010000460         2758 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_64319, whole genome shotgun sequence.
ACCESSION   DATMXK010000460 DATMXK010000000
VERSION     DATMXK010000460.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2758)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2758)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2758
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_64319"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(343..720)
                     /locus_tag="VN363_08480"
     CDS             complement(343..720)
                     /locus_tag="VN363_08480"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWQ84587.1"
                     /translation="MNTTQIIVTIGALALSLFIVWYFWFAPKGQIRAATGTGGAQEVA
                     VIVKGGYTPDVIVVKSGQPVRLHFTRQESSSCSEKVLFPDFNQNALLPEGQEVTLEFT
                     PDKPGEYGFQCQMGMLRGKLIVE"
     gene            complement(745..>2758)
                     /locus_tag="VN363_08485"
     CDS             complement(745..>2758)
                     /locus_tag="VN363_08485"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="copper-translocating P-type ATPase"
                     /protein_id="HWQ84588.1"
                     /translation="QQAEHENEYRRLMNRFWFAAIISIPVLITAYPQFIPIVRDWSME
                     TLRLAWAGAALLTLPVLFWSGSDFFTGAWAALKHRSANMNTLIALGTAAAWIYSTFAI
                     LFPAIFPEGTSEPFYDVVAVVIALVVLGQALELRAKGRTSEAIKKLMGLQAKTARVIR
                     DGQEMDIPVEEVLVGDVIQVRPGEKVPVDGILVDGSSAVDESMLTGESLPVSKKPGDE
                     VIGATLNKTGAFKFRATKVGKDTALAQIVKMVQDAQNSKAPIARMADTVSGYFVPVVM
                     ILAILTFVIWFDFGPQPQLVYALVTAVTVLIIACPCALGLATPMSLMVGIGKGAENGI
                     LIRSGEALQTAQGIKTVVLDKTGTITKGKPELTDTLVAVSWKENDLLHLAASVEKASE
                     HPLAEAIVEGAKSRNLPLSEAAAFEAVPGHGVIASVDGRRVVLGNPKMMTREGVALGD
                     FEQNAAALADDGKTPMYVAVDGQLAGVVAVADTVKEDSAEAIAALQKMGIEVVMITGD
                     NRRTAEAIARKVGITRVLAEVLPEDKAHNVHLLQAEGKKVAMVGDGINDAPALVQADV
                     GLAIGTGTDVAIEASDITLIKGSLKSVVTAIEVSRATMNNIKQNLVGAFFYNTLGVPV
                     AMGVLFPFFGVLLSPLLAGAAMAFSSVTVVGNANRLRTFKPRFGAK"
BASE COUNT          628 a          784 c          725 g          621 t
ORIGIN      
        1 ccacggccag cgtgatattt attcggtggt gtgattttga ttttatagcg aaccagggaa
       61 aggagcgagc gtcatccggc gcatttgggg atacgcagtt tggtgtatta tttcattcaa
      121 ccaaatatgg ctcaatcgaa ccaggctcac agacgaggag aattctaatt ttctaaagac
      181 aggctcaagc ggagaaagaa agcgggttct ccaaagaagg gcccatcgcg cttcgggttc
      241 agccggtata tactgatcac aaaagcgccg ttctgttcat aggggatatt acggttctcc
      301 ctatgagcag aacggcgtct tggaaaagta aaagtagaaa gtttattcga cgatcagttt
      361 gccgcgcagc atacccatct ggcactgaaa gccatattcg ccaggtttgt caggagtgaa
      421 ctccagggtt acctcctggc cttcaggcag cagagcattc tggttgaaat cggggaagag
      481 cactttctca gagcaggagg aactctcctg acgggtaaaa tgcaatcgca cgggctgacc
      541 agatttcact acaatcacat ccggggtgta gccgcctttg acgatcacgg ctacttcttg
      601 cgccccgccc gtacctgtag cggcgcgtat ttgccccttg ggggcaaacc agaagtacca
      661 gacgatgaaa agactgagcg ccagggctcc aatcgttacg atgatttggg ttgtattcat
      721 gtttgtttct ccaattgtat cgaactattt cgctccgaat ctcggcttga atgtgcgcaa
      781 acggttggca ttgccgacga cagtcacgct agagaaggcc atcgctgccc ctgctaacaa
      841 aggactgagc agtacgccaa agaaggggaa caatacaccc attgccactg gaacccccaa
      901 agtattgtaa aagaatgcac ctaccaggtt ctgcttgata ttattcatgg tagcccggct
      961 gacctcgatg gctgtcacga cacttttcag gctgcccttg atcagggtga tgtctgatgc
     1021 ctcaatggca acatccgtgc cagtacctat tgccaggcca acatcggcct gcaccagggc
     1081 tggagcatca ttgattccgt cgccaaccat agcaactttt tttccttcag cctgtaatag
     1141 gtgaacatta tgcgctttat cttcgggtag gacctctgcc agcacacggg tgatacccac
     1201 cttgcgagca atcgcctcgg ccgtgcgccg gttatcgcca gtaatcatca ccacttcgat
     1261 acccatcttt tgcagagcgg ctatcgcttc ggccgagtct tctttgaccg tatcagctac
     1321 cgccacaaca ccagccaact gcccgtccac ggctacgtac ataggcgtct ttccgtcatc
     1381 agccagggct gcagcgtttt gctcgaaatc gcccaaggcc acaccttcac gtgtcatcat
     1441 cttcgggtta cccaaaacaa ctcgccgacc atccacagaa gcgatcacac catgcccggg
     1501 aacggcttcg aaagcagccg cctcgctcag ggggaggttg cggctttttg cgccttcaac
     1561 gatggcttcc gctaacgggt gctcggaggc tttctccacc gaggctgcca ggtgcaacag
     1621 atcgttttcc ttccagctga cggcaaccag cgtgtcggtc agttcgggtt tgcctttggt
     1681 aatcgttccg gttttatcta acaccacagt cttgattccc tgggcggtct gcaaagcctc
     1741 gcccgagcgg atcaggatac cgttttcagc gccctttcca atgccaacca tcaggctcat
     1801 cggggtcgcc aggccaagag cgcacgggca ggcgatgatc agcacggtta ctgcagtgac
     1861 gagggcatac accagctgcg gctgcgggcc aaagtcgaac cagatcacaa aggtcagtat
     1921 ggccaggatc atcaccaccg gcacaaaata acctgaaacc gtgtcggcca tacgcgcgat
     1981 cggggctttg ctattctgtg catcctgcac catcttgacg atctgcgcca gggctgtgtc
     2041 tttgcccact ttggtagcgc ggaacttgaa ggcgccggtc ttgttgagcg tggcaccgat
     2101 cacctcatcg cctggtttct tggaaacagg cagtgattca ccggtgagca tcgattcatc
     2161 cacagctgag ctgccatcta cgagaatacc atccaccggg accttctcgc ctgggcgcac
     2221 ctggatcacg tcgcccacca ggacttcttc taccggaatg tccatctcct gtccgtcacg
     2281 gatcacacgc gctgttttgg cttgtaagcc cattaacttc ttgatggctt cgctggtgcg
     2341 gcctttggcg cgcagttcca gcgcctggcc cagcactacc agggcaatca caacggcgac
     2401 aacatcgtag aacggctcgg aggtgccttc ggggaagatg gccgggaaaa ggatagcaaa
     2461 tgtcgagtaa atccacgccg cagcggtgcc cagagcgatc agagtgttca tattagctga
     2521 gcgatgcttc aaggctgccc aggcgccggt aaagaaatca ctgcccgacc agaataacac
     2581 cggcagggtc aggagcgccg ccccagccca ggccaggcgc agcgtttcca tgctccaatc
     2641 ccgcacgatg gggatgaatt gtggataggc agtaatcaac actgggatgg aaatgatggc
     2701 tgcgaaccag aagcggttca tcagccggcg gtattcgttc tcgtgctcag cttgttgt
//
LOCUS       DATMXK010000461         6935 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_50686, whole genome shotgun sequence.
ACCESSION   DATMXK010000461 DATMXK010000000
VERSION     DATMXK010000461.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6935)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6935)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6935
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_50686"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1047)
                     /locus_tag="VN363_08490"
     CDS             complement(<1..1047)
                     /locus_tag="VN363_08490"
                     /EC_number="2.3.1.16"
                     /inference="COORDINATES: protein motif:HMM:TIGR01930.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003985 - acetyl-CoA C-acetyltransferase
                     activity [Evidence IEA];
                     GO_process: GO:0006631 - fatty acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl-CoA C-acyltransferase"
                     /protein_id="HWQ84589.1"
                     /translation="MNTNREAVIVAAVRTPVGKAKKGGLATVRPDDLAALVIREVLHR
                     ANDLDPTLVDDVIFGCAFPEGEQGMNMARLALIKAGLPVTVPGETINRFCSSGLQSIV
                     HAAYAILAGQAEIIIAGGAESMSMVPMTGYKFAPNPGLAADAPESYTAMGLTAELVAE
                     RYGISRTDQDLFALGSNQKAAAAVNSGRFDPELVPVSVDVVELAGNGQPAKRTFTVNR
                     DEGPRADTSLEALAKLRPAFKEGGSATAGNSSQMSDGASAVLVMSADKAQELGLKPLA
                     RFVAFAVGGVPPEVMGIGPIAAIPKALKLAGLTLEDIGLVELNEAFAAQSLAVIRELG
                     LDPEITNVNGGAIAL"
     gene            complement(1075..3498)
                     /locus_tag="VN363_08495"
     CDS             complement(1075..3498)
                     /locus_tag="VN363_08495"
                     /inference="COORDINATES: protein motif:HMM:NF014757.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="3-hydroxyacyl-CoA dehydrogenase/enoyl-CoA
                     hydratase family protein"
                     /protein_id="HWQ84590.1"
                     /translation="MTYKIHRAVVIGAGTMGAAIAAHLANAGVPVTLLDIVPTHLLPA
                     EEKQGLTLSDPAVRNRIVKEGLDRAIKSRPASFFTPETAVLVKVGNLEDDFDAIASAD
                     WVIEVIVENLTIKQQLMARIDVVRKPQTIVSTNTSGIPVSSIAGGLSEGFRQHFLGTH
                     FFNPPRYLKLLEIIPTADTNPEVVQAVSRFAEIRLGKGVVLAKDTPNFIGNRLAFGSG
                     AFALDYILTNGYTVDEVDAITGPALGRPKTATFRLIDLVGIDVWEHVGRNLAPAIPHD
                     EQALRYLNSTPVNQLIGTMVKNNWLGNKTKQGFYKEVRKEDGGKEFWSLNLNTLEYEP
                     PRKVRFESIGKARDKESLAERLKILLAGEDRAAQLARAITYQNLAYASERIPEIADTP
                     LPIDDAMRWGFGQEAGPFETWDMLGVKETVTAMQAEGFQPAAWVLKMLATGCETFYQY
                     DGERKEAAYSPSLGAYTPIARPAELFALKQLKDAGRVIRQNDSASLIDLGDGVACLEF
                     HTKMNSLDDDIFNLMSYAQAQAEKDYTGLVIGSDAQNFSAGANIFMVLMAARMGAWDQ
                     LSEVISKMQNINLRMRYSPVPIVVAPAGLALGGGAEVVMHASKVVAAAELYAGLVEIG
                     VGVIPAGGGTKEIVRRLVTPAMRTPNVDPLPHLQRAFEQIGLAKVATSAEEARQMGIL
                     SQGDRVVMHRDRLLAEAKREVLHLASSGYRPPPPEKLYAAGRDALAALRVGIYMMKEA
                     KFITEYESVIANKLAYILTGGELSQPAWVDEQLFLDLEREAFLSLCGEEKTQARIYNM
                     LETGKPLRN"
     gene            complement(3582..4910)
                     /locus_tag="VN363_08500"
     CDS             complement(3582..4910)
                     /locus_tag="VN363_08500"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560710.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWQ84591.1"
                     /translation="MTQIVVQILLILVLILINGVLSMSETAVVSARKARLQQRAEDGD
                     DKAKAALKLANEPSDFLSTVQIGITLVGILSGAFGGATVAEYLEIWMEAIPWLAPYAA
                     PLSLFLVVLVVTYLSLVFGELVPKQLALFNPEDVSSSVAAPMGKLARITAPLVRLLSA
                     STQSVLRIMGKASSNEPPVTEEEIKVMIGQGTSAGVFEQAEQHMVEGIFRLSDRRVGT
                     LMTPRTEVYWLDLEDSPETIRQTIFHSPYSNLPVARGNLDHVEGIVQAKDLLAQSLVS
                     EHLDVSGAIKLAQFVPESLPALQVLERFRESGIHIALVIDEFGGLQGLVTMMDVVEAI
                     VGELRSADGKEDSEIVRRADGSWLLSGMLPIDEFQAALGLKDLADFDRGGYETLGGFV
                     MAHMGRIPVAGDEFVINEIHYEVMDMDGMRVDKILVTPPAGEAQDTSFSI"
     gene            complement(4907..6043)
                     /locus_tag="VN363_08505"
     CDS             complement(4907..6043)
                     /locus_tag="VN363_08505"
                     /inference="COORDINATES: protein motif:HMM:NF012546.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ84592.1"
                     /translation="MNTQSALPSHWNLTKRKYWQVISLGLLLLGLVGCQAPGVPASPT
                     SPVTPVLPSSTPPAALPTPVAHNVSTAAEIPSSPTSAPSPTPTSTPDPYAPWTIDYLR
                     EREYGGGEIEVQETLQVTSYYTRYLISYPSDGLQIYGFANIPTGEGPFPVIIALHGYI
                     EPPLYQTLDYTTGYADTLARAGFLVLHPNLRGYRPSDEGENLFRVGMATDVLNLIALV
                     QTQAGQPGILEQADGEAIGLWGHSMGGGISTRVITVSPAVKAAVLYAAMSGDERQNYE
                     AINSWSYSIRGVEELAVPEEELTRISPIYFLENITAAVSIHHGESDELVPLGWSQDLC
                     ERLTLLEKRVECFTYPKMPHTFNGEGEQLFNQRVIEFFTRELQQ"
     gene            complement(6078..>6935)
                     /locus_tag="VN363_08510"
     CDS             complement(6078..>6935)
                     /locus_tag="VN363_08510"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWQ84593.1"
                     /translation="FGAMAMTESQAGSDTSAIRATAVLDKSTNEWVLNGEKIFVTGGH
                     ISLDLSDGFVVVWATIDPSAGRAGMRSFVVPAGTPGVAVTKLEHKLGIRASDTASIVL
                     QDARVPFDHILGSSEVVKPGAETKGFKGAMATFDATRPLVAAMAIGVARATVETLKEI
                     LAEQGVVIRYGMPRSKLTSIERDVIDMEVQLRSAWLLVLKAVWMADNRIPNSAEASMA
                     KVRAGDIVTRITQKALEIMGPMGYTTETLLEKWFRDAKISDIYEGTGQINRLVVARNI
                     LGYSGAELR"
BASE COUNT         1557 a         2019 c         1814 g         1545 t
ORIGIN      
        1 aagcgcaatc gcaccgccat tgacattggt gatctcggga tcgaggccca gctcacggat
       61 cacagccagg ctctgggcgg caaaggcttc attgagctca accagaccga tatcttccag
      121 ggttaaaccc gccaatttca aagccttggg aattgcggca attggaccga tacccatcac
      181 ctccggaggc actccgccaa ccgcgaaagc cacaaaccgc gccagaggtt tgagccccag
      241 ctcctgggct ttatcggccg acatgaccag cactgcggag gcgccatctg acatctggga
      301 ggagttgcca gctgttgctg aaccaccttc cttgaaagcc gggcgcagct tcgccagcgc
      361 ttccaggctg gtatcggcgc gcggcccttc atcgcgatta accgtgaaag tgcgcttcgc
      421 cggctgccca tttcccgcca gctcgacgac atctacggat accggaacca gctcaggatc
      481 gaagcggccg gaatttactg ctgcggctgc cttctgattg gagcccaggg cgaataaatc
      541 ctgatcggtg cggctgatcc catagcgctc ggctaccagc tcagcagtca ggcccatcgc
      601 agtataagac tcaggggcat ccgccgccag gcccggattc ggggcaaact tatagccagt
      661 catcggcacc atgctcatcg attctgcgcc accggcgatt ataatctcag cctgcccggc
      721 gagaatggca taggcagcat gaacgatact ctgcaaccca gaggagcaga agcggttgat
      781 tgtttcgccg ggaacggtca caggcaaacc ggctttgatc aacgccaggc gggccatgtt
      841 catcccctgc tccccttcag ggaaggcgca cccgaatata acatcgtcga ccagagtggg
      901 gtcaaggtca ttcgcccggt gcaggacttc acggattacc agcgcagcca gatcatctgg
      961 acgtacagtt gccagtccac ccttcttagc cttgccaacc ggcgtgcgca ccgcggcgac
     1021 aattactgct tctcgattcg tattcatcta cttctctcca attctgctgc atggctaatt
     1081 gcgcagcggt ttcccggttt caagcatgtt atagatgcga gcctgggtct tttcttctcc
     1141 acacagagat aagaaggctt ccctttccag gtccaggaag agttgctcat cgacccacgc
     1201 aggctggctc agctcaccgc cggtgaggat atacgccagc ttattggcaa tcacggattc
     1261 atactcggtg atgaatttag cttctttcat catatagatg ccaacccgca aggcagccag
     1321 ggcatctcgt ccagcggcat aaagtttctc tggaggcggc ggacggtagc cagaggaagc
     1381 cagatgtaaa acttcccgct tggcttctgc aagcaaccgg tccctatgca taaccacccg
     1441 gtcaccctgg ctgaggatac ccatctggcg agcttcttcc gcactggtag ccactttcgc
     1501 cagtccaatc tgctcaaatg cccgctgtaa atggggcagc ggatcgacat tcggggtgcg
     1561 catggcaggc gttaccagcc ggcgtacgat ctcttttgtt ccaccacccg ccgggataac
     1621 ccccacgccg atctcgacca ggccggcata cagctcggcg gctgcgacta ccttgctggc
     1681 atgcatgacc acttctgctc cgccccccag agccaaacct gccggggcaa ccacgatcgg
     1741 gacgggtgag tagcgcatgc gcagattaat attctgcatc ttggaaatta cttcgctgag
     1801 ctgatcccag gctcccatac gcgccgccat gagcaccatg aaaatattcg ctccggcgct
     1861 gaagttctga gcatcactgc cgataaccag cccggtataa tccttctcag cctgagcctg
     1921 ggcatagctc atcaagttga agatatcatc atccagcgaa ttcatcttgg tatggaactc
     1981 aaggcaggcc acgccatcac ccagatcaat caggctggcg ctgtcattct ggcgaatgac
     2041 tctgccggca tctttgagct gtttgagggc aaacaactca gcaggtcgcg cgattggggt
     2101 ataggcaccg aggctcgggc tgtacgcggc ctctttccgc tcaccatcat actggtagaa
     2161 cgtctcgcat ccggtagcta acatcttaag cacccaggct gccggttgaa aaccttcagc
     2221 ctgcatggct gtgaccgttt cctttacccc cagcatgtcc caggtctcaa acggtccagc
     2281 ctcctgcccg aatccccagc gcatggcatc atcgatgggc aagggtgtat cggcaatctc
     2341 ggggatgcgc tcagaagcat acgccagatt ttgataggtg atcgcccgcg ccagctgcgc
     2401 agcgcgatcc tctccagcca aaaggatttt caatcgctcg gcgagggatt ctttatctct
     2461 ggcttttccg atcgattcga agcggacttt cctgggaggc tcatattcca gcgtgttcag
     2521 gttcaacgac cagaactctt tgccaccatc ttccttacgc acttctttgt aaaaaccctg
     2581 tttcgttttg tttcccagcc agttgttttt aaccattgtg ccaatcagct ggttgaccgg
     2641 ggtggagttc agatagcgca gtgcttgctc gtcatgcgga atcgctggtg ccagattgcg
     2701 accaacatgc tcccagacat cgatcccaac gaggtcgatg aggcgaaatg tcgcagtttt
     2761 agggcgcccg agtgcgggac cggtgatggc atccacttca tcaaccgtat agccgttggt
     2821 gaggatgtaa tcgagggcaa aagcaccact gccaaatgcc agacggttgc cgatgaaatt
     2881 tggggtatct tttgccagca ccacgccttt gccgaggcgg atttcagcga agcggctgac
     2941 ggcctgaaca acttcaggat tggtatctgc tgttgggatt atttccaaca actttaagta
     3001 gcgcggtgga ttgaagaagt gagtacctaa gaagtgctgg cgaaaacctt ctgacagacc
     3061 tccggcgatt gatgaaaccg gaataccaga ggtattggtg ctgacgattg tctggggttt
     3121 acgcacaaca tcgatgcggg ccatgagctg ctgcttgatc gtcagatttt cgacgatcac
     3181 ttcaatgacc caatcagcac tggcgatcgc atcaaaatca tcttccagat ttcctacttt
     3241 caccaatacg gccgtctcag gggtgaaaaa actggccggg cgcgatttta tagcccggtc
     3301 caggccttcc ttaacgatcc ggttgcggac cgcaggatca ctgagcgtca ggccttgttt
     3361 ctcttctgcc ggaagcaggt gagtggggac aatatccaac aacgtaaccg gcacaccggc
     3421 atttgccagg tgtgcggcta tagccgcccc cattgtgccc gctccaatca ccactgcacg
     3481 atggattttg tatgtcatcg tttctcctct ctttgatttg aatccctctc ttggatccgc
     3541 cctctttatc ttttgatcct gattattaag agagtggatc actagattga gaagctggta
     3601 tcctgggctt ctccagccgg aggtgtgacc aatattttgt ctacccgcat accatccata
     3661 tccatcactt cataatggat ttcgttgatt acgaattcat cgcctgcaac ggggatccgc
     3721 cccatatgag ccatgacaaa tccacccaga gtctcataac caccgcgatc gaaatcggcc
     3781 aaatccttca agcccaatgc agcctgaaac tcatcgatgg gcagcatacc tgaaagcagc
     3841 catgagccat ccgcgcggcg gacgatctcc gaatcttctt tgccatctgc agaacgaagt
     3901 tcaccaacga tcgcctcaac cacatccatc atcgtgacca ggccttgcaa accgccaaac
     3961 tcatcgatca ccagtgcgat atggatcccg ctttcccgga aacgttccag cacctgcaaa
     4021 gccggtagac tctccggtac aaattgagcc agcttgatcg cacctgaaac atcgagatgc
     4081 tcgcttacca gtgattgagc taacaggtct ttcgcctgga caatcccttc cacatgatcc
     4141 agattacccc tggcgaccgg aagatttgag taagggctgt gaaaaatcgt ctggcggatc
     4201 gtttctggcg agtcttcgag atccagccag taaacctcgg tgcgaggggt cattaacgtt
     4261 cccactcgcc ggtcgctcag tcgaaagatg ccttcaacca tgtgctgttc agcctgttcg
     4321 aaaaccccgg cactggtgcc ttggccgatc ataaccttga tctcttcttc ggtcacgggg
     4381 ggttcgttag aacttgcctt gcccatgatt cgcaagactg actgagtgga ggcactgagc
     4441 aggcgaacca ggggggcagt tatgcgagcc agctttccca ttggcgctgc gacactggat
     4501 gaaacatctt cgggattgaa aagcgccagc tgcttgggaa ccagctcccc aaaaaccagg
     4561 ctcaagtagg tcacaacgag cacgaccagg aacagactga gcggagcagc atacggcgcc
     4621 agccatggga tcgcctccat ccagatttca agatattcag cgactgtggc cccgccaaac
     4681 gcgccggaca agatccctac cagggtgatg ccaatctgga ctgtcgacag aaagtcggac
     4741 ggctcattcg ccagtttgag agcagctttc gctttatcat cgccatcctc ggcgcgctgc
     4801 tgtaagcgag ccttgcgggc ggaaacaacc gctgtctctg acatcgagag gacaccattg
     4861 atcaggatta ataccaggat gagcaggatc tggaccacaa tctgggtcat tgctgcagct
     4921 cgcgggtaaa gaactcgatt acgcgttggt taaacaattg ttccccttca ccgttgaagg
     4981 tgtgcggcat tttcggatag gtgaaacatt ccacccgctt ctcaagtaag gttaggcgct
     5041 cgcataaatc ctgggaccac cccaatggca ccagctcatc agattcgccg tgatggatgg
     5101 aaactgcggc agtgatattc tccagaaagt agataggcga gatgcgggtc aattcttctt
     5161 ccggaacagc cagctcttcc accccgcgga tagaatagga ccagctgttg atggcttcat
     5221 aattttggcg ctcatcgcca gacatcgctg cgtagagcac tgcagccttg actgctggac
     5281 tgacagtaat cacccgggta gatattcctc cccccatact atgcccccac aggccaatcg
     5341 cttctccatc cgcctgttcc aggatgcccg gctgaccggc ctgggtctga accaaagcga
     5401 tcagattgag cacatcggtg gccattccca cccgaaagag attctcaccc tcgtccgagg
     5461 ggcgataacc gcgtaaattg ggatggagca ccaggaatcc ggcgcgtgcc agagtatcag
     5521 catagcccgt cgtataatcc agggtctggt aaaggggcgg ctcgatataa ccatgcaggg
     5581 caatgatcac cgggaaaggg ccttcgccgg ttggaatatt ggcaaaacca taaatttgca
     5641 gtccatcgct gggatagctg atcaggtacc gtgtgtagta tgaagtaacc tgcaaggttt
     5701 cctgtacttc gatctcgcca cccccgtact ctcgctcacg cagataatcg atcgtccatg
     5761 gagcgtaagg atccggagtg gaagttggcg tggggctggg tgcagaagtt ggactggaag
     5821 gaatctccgc agccgtggaa acgttatgcg ctaccggggt tggcagtgca gctggcggcg
     5881 tactgctggg taaaacaggc gttacaggac tggtgggact cgcaggcaca ccgggagcct
     5941 ggcaccctac cagtccaagc agcaagagcc cgaggctgat tacttgccag tattttcgct
     6001 tggtaaggtt ccaatgcgag gggagagcag actgagtgtt catcccctca ttttaccaaa
     6061 tttggattaa catccgttta gcgcagctct gccccactgt atcccaggat attccgcgct
     6121 acaaccaggc ggttgatttg cccggtacct tcatagatat cgctgatctt ggcatcccgg
     6181 aaccactttt caagcaaggt ttcggtggtg taacccatgg gtcccatgat ttcgagggct
     6241 ttctgggtga tgcgagtaac gatatcgccg gcgcgcactt tggccatcga agcttcggca
     6301 gaattaggga tgcggttgtc cgccatccac accgctttga gaaccaacag ccaggcagag
     6361 cgcagctgga cttccatatc gatgacatcc cgctcgatgc tggtcagctt gctgcgaggc
     6421 attccatagc ggataacgac tccctgctct gccaaaattt cttttagggt ttcgacggtt
     6481 gctcgcgcca caccgatcgc catcgcggcc accagggggc gggtagcatc aaaagtcgcc
     6541 atggcgcctt tgaagccttt ggtttccgct ccaggtttga ctacctcgga actgcccagg
     6601 atatggtcaa atggtacgcg ggcatcttgc agaacaatag aagcggtgtc gctggccctg
     6661 atcccgagct tgtgttccag ctttgtgacc gcaacacccg gagtaccggc tggaacgacg
     6721 aaggagcgca tgccggctcg gcctgcgctg ggatcaatcg tcgcccaaac gaccacaaaa
     6781 ccgtccgaca gatcgaggga gatatgcccc ccggtgacaa aaatcttttc tccgttcaga
     6841 acccattcgt tggttgattt gtcaaggacc gcggtagccc ggatcgccga tgtatccgag
     6901 ccagcctgtg actcggtcat cgccatcgcc ccgaa
//
LOCUS       DATMXK010000462         2958 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_99398, whole genome shotgun sequence.
ACCESSION   DATMXK010000462 DATMXK010000000
VERSION     DATMXK010000462.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2958)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2958)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2958
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_99398"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1004
                     /locus_tag="VN363_08515"
     CDS             <1..1004
                     /locus_tag="VN363_08515"
                     /inference="COORDINATES: protein motif:HMM:NF014871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_process: GO:0009166 - nucleotide catabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="5'-nucleotidase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ84594.1"
                     /translation="HTQYILNRPDGGLVVENPNSGVRFIRVRLVVDTSTKAVIYKTAD
                     FHKPWNIGVTPDPAIQMYIDELNAQIAPIMATVIGSAAKYIPRADTCGRSDGRLCESL
                     VGNVSTDSMRLAYNTDFAITNAGGLRADLTCPTVDLAGDFCPPYTPPPYPITRGSVLG
                     VLPFGNIVVTVSISGEELKAFLENGVSSMPSAAGKFPQVSGLCFTYDIAAPAGSRVLS
                     AVRQDPVGNCTAIPVDLTAASTYTLATNDFVANGGDGYPNVYSRVTTQNYMDQVLADY
                     ITANSPVNPFVNGYPAGRINCVGIGCPLQTPSPAVLAAAMKNLLDWGSTLFLPLTTH"
     gene            1196..1660
                     /locus_tag="VN363_08520"
     CDS             1196..1660
                     /locus_tag="VN363_08520"
                     /inference="COORDINATES: protein motif:HMM:NF025170.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PCYCGC motif-containing (lipo)protein"
                     /protein_id="HWQ84595.1"
                     /translation="MTLKSHRIPLLLVLFILLSGGLTACAGDAASGESSHQTAEMPMA
                     SLDDMPQEVKDAPVSVQQAYQFNAANPDVMTEIPCYCGCGSMGHTSNYDCYVADVAAD
                     GAITYDSHALGCSICVDITQDSMRLLKQGKTVAEVKADIDRTYAQYGPSNIP"
     gene            1670..2137
                     /locus_tag="VN363_08525"
     CDS             1670..2137
                     /locus_tag="VN363_08525"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWQ84596.1"
                     /translation="MDQSTATRIKLPRWLAFAVLAGLAALILWLPLPARAAPPTERIF
                     RIEASRWAYSPAVLAVNPGDRVTIELAATDVVHGLGIDGYHVATTSDPGQTSRMTFVA
                     DQAGTYRFRCTVTCGNLHPFMIGKLQVGENSLLWRGRLLAVLALLAAFWTVRR"
     gene            2134..>2958
                     /locus_tag="VN363_08530"
     CDS             2134..>2958
                     /locus_tag="VN363_08530"
                     /inference="COORDINATES: protein motif:HMM:NF024211.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWQ84597.1"
                     /translation="MKTLPEITWRVADSAPPAGPQTGATGGEPAAPVRPAAPWRLNLA
                     RLPWLRPLLLRREPQLLLRAITLAGFAFTILVGLFGSPVGSHNFAIIFVWIAWWSALK
                     LVFIPFGGKSWCSVCPIPLPGEWLQSGGILQKTRRRFGLNKRWPKRLRGSWLQAGAFL
                     LIGLFSAVTLTDPRMTAWVLLGLFGLAMAFSLVFEKRSFCTNLCPIGGFSGIYARTAP
                     IELRVIEPAVCAAHTEKTCYQACPWGLYPLALKDSSQCGLCMECLRVCPSDNIAVNL"
BASE COUNT          503 a          969 c          855 g          631 t
ORIGIN      
        1 cgcacaccca gtacatcctc aaccggccgg acggcggtct ggtggtagag aatcccaatt
       61 ctggcgtgcg cttcattcgt gtgcgcctgg tggtggatac cagcacaaag gccgtgattt
      121 acaagactgc tgatttccac aaaccctgga atatcggcgt gacccccgat ccagccatcc
      181 agatgtacat cgacgagctg aatgcccaga ttgcgccaat tatggcgacc gtgatcggca
      241 gcgccgccaa gtacatcccg cgggcggata cctgtgggcg cagcgatggc cggctgtgtg
      301 aatcgctggt cggaaacgta tccaccgatt cgatgcgttt ggcttataac acggatttcg
      361 ccatcaccaa cgccggcggg ctgcgcgccg acctgacctg ccccaccgtg gatctggcag
      421 gcgacttctg cccgccttac accccgcctc cgtatcccat cacgcgtggc tcggtcctgg
      481 gtgtgctgcc cttcggcaat atcgtggtga cagtcagcat cagcggagag gagctgaaag
      541 ccttcctgga gaacggcgtt tcgtccatgc cttcggccgc cgggaagttc ccgcaggttt
      601 ccgggctgtg cttcacctac gatatcgccg ctccagccgg cagccgcgtg ctgagtgcgg
      661 tgcgccagga tcctgttgga aactgcaccg ccattccggt tgacctgacc gccgcttcca
      721 cgtacaccct ggcgaccaac gactttgtgg cgaacggcgg cgatggttac ccgaacgtgt
      781 acagccgggt caccacccag aattatatgg accaggttct ggcggattac atcaccgcca
      841 actcgccggt gaacccgttc gtcaacgggt accccgccgg acggatcaac tgcgtcggta
      901 taggctgccc actgcagact ccctccccgg ctgtgctggc ggcagccatg aaaaacctcc
      961 tggattgggg ttccaccctg tttttgcccc tcaccaccca ttaggccagg ctgccggcac
     1021 agctctcagc cttgcctctc ccaatccccg ttgtgcgttg tgcggaggtc accagcctcc
     1081 gcacaaccct ttaacaggca attatgccta atcaaaaccg gtcatcttgc gctgtgcttt
     1141 cttcgctggt atgcctataa tctggatacg atatttaatg gtggagaagc tgtcaatgac
     1201 cctcaagtct catcgtatcc ctctcttgct cgtactattc attcttctgt ctggtggact
     1261 caccgcttgc gctggcgatg cagccagtgg tgaatcctcc caccaaaccg ctgaaatgcc
     1321 catggcctcc ttggacgaca tgccccagga ggttaaagat gcccccgtct ccgtccagca
     1381 ggcttaccag ttcaacgccg ccaacccgga tgtcatgacc gagatcccct gctactgcgg
     1441 ctgcgggtcg atggggcaca cctcaaatta cgactgctac gtggcggatg tggctgccga
     1501 tggcgccatc acctacgatt cgcacgccct gggctgttcg atctgtgtgg atatcaccca
     1561 ggacagcatg cgcctgctga aacagggcaa aacggtcgcc gaagtaaagg cagatatcga
     1621 ccgcacctat gcccagtacg ggccgtcgaa tattccctga gcctgaacaa tggatcaatc
     1681 gactgcaacc aggatcaagc tgccccgctg gctggctttt gctgtgctgg cgggtctggc
     1741 cgcgctgatc ctctggctgc cgctgcctgc cagggcagct ccgcccaccg agcgcatctt
     1801 ccggattgaa gccagccggt gggcgtattc gcccgccgtc ctggcggtta atcctggcga
     1861 ccgggtgacg atcgagctgg ctgccacgga tgtggttcac ggcctgggaa ttgacggcta
     1921 tcacgtcgca acgacttctg atcccggtca gacctcccgc atgacgttcg tcgcggatca
     1981 agccgggacc taccgctttc gctgcacggt tacctgcgga aacctgcacc cctttatgat
     2041 tggcaagctc caggtcggtg agaattccct gctgtggcgc ggccgcctgc tggctgttct
     2101 ggcgctgctg gcagccttct ggacggtgcg ccggtgaaga ctctgcccga gatcacctgg
     2161 cgcgtggctg acagcgcgcc tccggccggt ccgcagaccg gcgccaccgg cggtgagcct
     2221 gccgcgcctg tgcgccctgc ggctccctgg cggctgaatc tggcccgcct gccctggctg
     2281 cgcccgctgc tgctgcgccg tgagccgcag ctcctgctgc gtgccattac cctggcggga
     2341 ttcgcgttta cgatcctggt cgggttgttt ggctcgccgg tgggcagcca caatttcgcc
     2401 atcattttcg tttggatcgc ctggtggagc gcccttaaac tggtcttcat ccccttcggc
     2461 gggaagtcct ggtgcagtgt gtgccccatc ccgctgcctg gcgagtggct gcagtccggt
     2521 ggcatcctcc aaaaaacccg gagacggttt ggcttgaata aacgctggcc gaaacggctg
     2581 cgcggctcct ggctgcaggc cggggctttt ctcctgattg gcctgttcag cgcggtcacc
     2641 ctcaccgatc cacggatgac tgcctgggtt ctgttggggc tgttcgggct cgccatggca
     2701 ttcagcctgg tcttcgaaaa gcgcagtttc tgcaccaacc tgtgcccgat cggaggcttt
     2761 tccggtatat atgccagaac cgctcccatc gagctgcggg tgatcgagcc tgccgtatgc
     2821 gccgcccata ctgagaaaac ctgctaccag gcctgcccgt gggggctgta cccgctggcg
     2881 ctgaaagaca gctcgcagtg cgggctgtgc atggaatgcc tgcgcgtctg cccgtccgat
     2941 aatatcgccg tcaacctg
//
LOCUS       DATMXK010000463         6690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_164081, whole genome shotgun sequence.
ACCESSION   DATMXK010000463 DATMXK010000000
VERSION     DATMXK010000463.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6690
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_164081"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..3401)
                     /locus_tag="VN363_08535"
     CDS             complement(<1..3401)
                     /locus_tag="VN363_08535"
                     /inference="COORDINATES: protein motif:HMM:NF013736.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ84598.1"
                     /translation="MSKRQLNSRIKDLFAELSDEPLVSQGQDTARLAGWTWEADGHGI
                     YTAISPEIKSFLGYSGESLVGQPVTEGLEAGSDLNLQRALESSQFPAELQLNYLTNSG
                     NFLATNIELFPIPWQPDEPFQREKNLGWRAIVHPAAPELPQHHRVEGQIEALNQAKAE
                     KPEPRIRKKTPGISLPAAPGFPADWLNQPAAAPRGYLAEDQHVQHSQEILTDLGWQSL
                     ADNQPARRPAESGEQAILAFSTQLPDGNTSLLLELLDEDAGRDWNASERMLVEQVASQ
                     LTLALENAQLFQQTQNALAATSALYAASAAFSAAHSHAEILKALQQFSQLGQADKLLA
                     IHYFDHPWSETQPALRSELLSGWAPSRSEIKQLTLRLDDPQSTQFLFADRITILNNLS
                     PFNSKSDAITLLRDRFQLDTLVCIPLVTGGEWLGFVSAGYSAAVHFSAEDELRLMALS
                     SQAAIAINNLQLLAETRQRNQELAAINRIVTAVAGSKDLQSSLGIIAAEMGISLKVQT
                     GIALLNEERQVMTIVASYSPIPNAPEVIGIDLPIQGNPSTQQVLATRKTLVVEDAQHN
                     PLTSAIHNELHLRSIESLMIIPLIVDERVIGTVGLDILEPSRHYTPDEIRLAETIVLQ
                     ASTAIQSTRLFEQTELALAETETLYKASAEFNHAESYAQVLEILRRFTMVGHAATTSL
                     LALFEEPQLIGYGPLVIPDWYLPIATWSASSAEVFTARRLPIQDFPAANELLKPDAPS
                     ILFDVENDLHLEYPARQIYSQRYQAKSVLFAPLVTGGHWIGFLGTFFNHPKALSNPDI
                     RRLMVLTGQASVAIQNIRLLEESRRRADQLQTAAEVARDTSSTLALDALLLRVVNLLC
                     DRFGYNHASVFLLDEDGIYAVVREATGEAGAEMKHRPHKLGVGSQSVIGTVTRNGLPL
                     VINDVSHSSVHRPNPLLPDTRAELGIPLKIGQRVIGALDVQSNEINAFTPDDVAVLQV
                     LADQIAVAVDNARAYMISQQAVEEMREVDRLKSQFLANMSHELRTPLNSIIGFSRVIL
                     KGIDGPLTDLQEQDLSAIYNSGQHLLNLINDVLDLSKIEAGKLELAFDDKVNLPDLIN
                     SVMSTVVGLVKDKPIQLTRNISANLPSVRADPTKIRQVLINLFSNA"
     gene            complement(3370..4149)
                     /locus_tag="VN363_08540"
     CDS             complement(3370..4149)
                     /locus_tag="VN363_08540"
                     /inference="COORDINATES: protein motif:HMM:NF018290.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ParA family protein"
                     /protein_id="HWQ84599.1"
                     /translation="MTCIIAVGNQKGGVAKTTTVMSLGGALAEMGHAILLVDLDAQAN
                     LTLSSGVDPSKLRGTIADVLLQSANLYSVSRETSIPGLDIVPANAEMDLAERFLPVRN
                     NYQSILRSALDGQTAYDYVLLDCPPSIGAVTQNALNAAQLLIIPTQAEYFSAHALRAM
                     LGTTRRIRQQNNPALETRILITMFDQRNRIHREISEQLRSTFGDSLFDTYIQVDTKLR
                     ESAVAGVPITHFLNHTRGTLQYRALAQEILQHVQATAQQPD"
     gene            complement(4146..>6690)
                     /locus_tag="VN363_08545"
     CDS             complement(4146..>6690)
                     /locus_tag="VN363_08545"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ84600.1"
                     /translation="MVTGQSIWLSSLESVNYQGGFLPLIAYTQGSFAQIIWPVSIAGA
                     GTLLILTLVFLSFFDKQVFPSARSLTRLLLIAQIIGVLLNVGLANRLQPSLPMLLTNL
                     LLAVVFSYASFRQLASERLLQRGPLQVRLTILILWVTFPIIAFMISFLTMQSSQRIEQ
                     GALARLEANNLSLNSQLEQWLDGEIRAYQDVLNQPGLRSLPTSDQSLALQALSDTHPW
                     LNYAGTADLSGGLVASSGTSPFRSISELSGYQQVIGGAETAVHVWDEPATPGSELIIA
                     LPIHAPGQPLTGVGLFSISLEALSAQLTPEMTPVLGEALVLDQANRIILQIPPEPTLL
                     GTDISNQAVIAEPQAGSAEPFRYTREDGDVMRAVASSTSYNWTIITQESEASLLAPVR
                     LLHRAAWPLAIGGALIMGFLVWISIRHTLHPIATLSNVADNIAGGNFSRSVPVESEDE
                     LGNLAQSMNSMIAQMRGLITNLEQHVAERTQELQHRAIQLQVAADVASESASIHELER
                     VLDHTVHLISERFNFYHTGIFLIDDAGEYAVLRAASSDGGQRMLARRHKLRVGQTGIV
                     GFVAKKGESRVASEVVQDAVYFDNPDMPQTRSEAALPLKVRGVVTGVLDVQSTEAEAF
                     KAEDIQILQVLADQIALAINNARLFDENRQAMDELQSLYSNQVREAWKSQLEDRSIGY
                     VYDRLSVQPLHEPSQASVPGEPEDPFRIEAPIELRGARLGNLSLKRDPNQVPWSQQDR
                     ELLLESARQIALALENARLLEETRQRAERERIAGQISTRLRMSNDPQQILQTAVSELK
                     QALHAHKAQALIAPRSPKPDPSIQSSDNPEKPPAETRGKGGNGMNTGGL"
BASE COUNT         1473 a         1773 c         1900 g         1544 t
ORIGIN      
        1 gcattcgaga acaggttgat taaaacctgc cggatcttgg ttggatccgc gcgtaccgaa
       61 ggcaagttgg cactgatatt ccgggttagc tggatcggtt tatctttcac caacccaacc
      121 acggtggaca ttacgctgtt gatcagatct ggcagattaa ctttgtcatc gaaggcaagc
      181 tccagtttgc cagcctcgat cttggaaaga tcgagcacat cattgatcaa gttcagcaga
      241 tgctgaccag aattgtatat cgcagagagg tcctgttcct gtagatcggt cagtggcccg
      301 tcgatcccct tcaagatcac ccgcgagaag ccgatgatcg aattcaatgg tgtccgcagc
      361 tcatggctca tattcgccag gaactggctt ttcaagcgat cgacctcgcg catctcttca
      421 accgcttgtt gcgagatcat atacgcccgg gcattatcca cggctaccgc aatctggtcg
      481 gcgagcacct gaagcacggc tacatcatcc ggcgtaaagg cattgatttc attggattgg
      541 acatccaatg ctccaatcac gcgctggccg atttttaacg gaatacccag ctcggcgcgt
      601 gtatcgggga gcagtgggtt gggtctatgg acggaactgt ggctgacatc gttgatcacc
      661 agtgggagtc cattgcgggt taccgtcccg atcaccgatt gtgatcctac ccccagtttg
      721 tgtggcctgt gtttcatttc tgccccggct tcgccggttg cttcgcgtac caccgcgtaa
      781 atgccatcct catccagcaa aaagaccgaa gcgtggttat aaccaaaccg gtcgcagagt
      841 agatttacca cccgcagcag gagggcatcc agggccaggg ttgatgatgt atctcgggcg
      901 acttcagccg cggtttgcag ctggtctgca cgccggcggc tttcttccag caggcgaata
      961 ttttggattg ctacagatgc ctggcctgtc aataccatta agcgccgaat atcggggttg
     1021 gacagcgcct tggggtggtt gaaaaatgta cccaggaacc cgatccaatg cccgccggtc
     1081 accagaggag caaataaaac tgattttgcc tggtagcgct ggctgtagat ttgcctggcg
     1141 ggatactcca ggtgcagatc gttttcaaca tcaaagagga tacttggagc gtctggcttc
     1201 aggagctcgt tcgctgccgg gaagtcctgg atcggtaaac gcctggcggt aaaaacctca
     1261 gccgagctgg cggaccaggt cgcgattggc aaataccaat cggggataac cagtggacca
     1321 tagccaatca attgaggttc ttcaaagagt gccaataagc tggttgttgc cgcatgtcca
     1381 accatggtaa agcggcgcag gatttccagg acttgcgcat agctttctgc atgattgaat
     1441 tccgcactgg ctttatacag ggtttcagtt tcagccagtg ccagctcggt ttgttcgaac
     1501 aggcgcgtgc tctggatcgc tgtcgaagcc tgcagtacga tggtttccgc caggcggatc
     1561 tcatccgggg tgtagtggcg tgacggctcg aggatgtcca gcccgacagt tccaatcaca
     1621 cgctcatcca caatcaacgg aatgatcatc aaagactcga tcgagcgcag atgaagttcg
     1681 ttgtgaattg cagaggtcag cgggttgtgt tgggcgtctt ccacaacaag cgttttacgg
     1741 gtagcaagga cctgctgggt tgagggattt ccctggatgg gtagatcgat cccaatcacc
     1801 tccggtgcat tggggattgg actatagctg gcaacgatgg tcatcacctg gcgctcttcg
     1861 ttcagcaagg cgatcccagt ttgaactttc aggcttatac ccatctctgc ggcaatgatc
     1921 cccaaactgg attgcagatc tttagagccg gcgacagccg ttacgatccg gttgatcgca
     1981 gccagttcct ggttccgctg gcgggtttca gccaataatt gcaggttatt gatcgcaatc
     2041 gctgcctggc tggaaagcgc catcaggcgg agctcatctt cggcgctgaa gtggacggct
     2101 gcactgtatc ctgcactgac aaagcccagc cattcaccac cggtgaccag cggaatgcat
     2161 accagtgtat cgagctgaaa acgatctctc aacagcgtga tggcatccga tttagaatta
     2221 aatgggctca gattattcag gattgtgatg cgatcagcaa acagaaattg tgtgctttga
     2281 gggtcatcca ggcggagtgt gagttgtttt atctcgcttc tggaaggcgc ccatccgctg
     2341 agcagctcac ttcgcagtgc tggctgcgtc tcgctccacg ggtggtcgaa ataatggatc
     2401 gccaggagtt tatcagcctg tcccagctgg gaaaactgct ggagagcctt caaaatttcc
     2461 gcatgagagt gggcggcact gaaagcagcg cttgcggcat ataaggcgga agtagctgcg
     2521 agtgcgtttt gagtctgctg gaagagctgg gcattttcca gggcgagagt cagctggctg
     2581 gctacctgct cgactaacat ccgctcggaa gcgttccaat cgcgccctgc gtcttcatcg
     2641 agcagctcga gcagcaggct cgtattgccg tcaggaagct gcgtggagaa agccagtatg
     2701 gcttgctcgc cggattccgc tggacggcgg gcaggctggt tatcagccag gctttgccag
     2761 cccaggtcag ttaagatttc ctggctgtgc tgtacatgct ggtcttcagc caggtatccc
     2821 cgcggcgcgg cagccggttg gttcagccag tctgcaggga atccaggtgc agccggcagt
     2881 gagatgccgg gtgtcttttt gcgtatccga ggttccggct tttccgcttt tgcctgatta
     2941 agagcttcga tctgcccctc tacacgatgg tgttgaggca attcaggcgc cgccgggtgc
     3001 acgattgctc gccacccgag gtttttttct ctctggaagg gctcgtctgg ctgccagggt
     3061 atggggaaca actcgatgtt tgttgcgaga aaattaccag aattcgtaag gtaatttagc
     3121 tgcagctcgg caggaaattg actgctttcc agcgcgcgct gaagattcag gtctgatcct
     3181 gcttccagcc cttcggtaac cggttggcca accaggctct cacccgaata tcccaggaag
     3241 cttttgatct cggggctgat ggcagtgtaa atcccgtgcc catctgcttc ccaggtccag
     3301 cccgccagcc gcgcagtgtc ctgcccctgg cttaccagag gctcatcgct cagctcagcg
     3361 aacagatctt taatccggct gttgagctgt cgcttggaca tgctgaagga tctcctgcgc
     3421 cagagcgcgg tattggagtg taccccgggt atggttgagg aaatgggtga tcggtacgcc
     3481 ggccactgcg ctttcacgca gtttggtatc cacctggata tacgtatcaa agagcgagtc
     3541 tccgaaagtt gaacggagct gttccgaaat ttcgcgatgg atccggttgc gctggtcaaa
     3601 catcgtaatc aggatccggg tctccagggc cggattgttt tgctggcgga ttctgcgcgt
     3661 cgtgcccagc atcgctctca aggcgtgagc tgaaaaatat tccgcctgag tggggatgat
     3721 cagaagctgc gctgcgttca aagcattctg agtgacggct ccaatcgaag gcggacaatc
     3781 caacaagaca taatcatagg cagtttgccc atccagagca gaccgtaaaa tggattggta
     3841 attattccgg acgggcaaga agcgctctgc caggtccatt tctgcgttgg cgggaacgat
     3901 atccagccct ggaatacttg tctcccggct cacgctgtac aggttcgccg actgcagtaa
     3961 gacatcggca attgtccccc gcagcttact gggatccacg ccggaagata gggtcaaatt
     4021 tgcctgagca tccagatcta ccagcagaat ggcatgcccc atctcggcta aagctccccc
     4081 cagagacatg acggtggtgg tttttgccac cccgcctttt tgattcccga ccgcgataat
     4141 gcatgtcata agccacccgt attcatgcca ttgccacctt tgcctcgcgt ttcagctggt
     4201 ggcttttcag gattgtctga ggattgaatc gacgggtcgg gtttcggcga acgcggcgcg
     4261 atcagcgcct gggctttatg ggcatgcagg gcttgcttga gttcgctgac tgctgtttgc
     4321 aggatttgct gcgggtcgtt gctcatccgg agacgtgtgg atatttgccc agcaatccgc
     4381 tcacgttcag ctcgttggcg ggtttcctcc aggaggcggg cattttccag agccagggca
     4441 atttgcctgg cagattccag gagcagctca cgatcctgtt gtgaccaggg cacctggttg
     4501 gggtcacgtt taagagaaag gttccccagg cgagcgccgc gcagctcgat aggcgcctca
     4561 atcctgaagg gatcttcagg ctcgcccgga acactggcct ggctgggctc gtgtagtggc
     4621 tgcacactca agcggtcata aacatatcca atcgaacggt cttccagctg gcttttccag
     4681 gcttctcgaa cctgattgct gtaaagggat tgcagttcgt ccatcgcctg gcggttttcg
     4741 tcgaaaaggc gggcattatt gatggcgagg gcaatctggt cggccagcac ctgcaggatc
     4801 tggatatctt cggctttgaa tgcttctgcc tcagtggatt ggacatccag gacaccggtc
     4861 acaaccccgc ggactttcag aggcagggct gcctctgagc gagtttgagg catatccggg
     4921 ttatcgaaat acactgcgtc ctgcacaacc tccgaggcga cgcgactttc tcctttttta
     4981 gcgacaaaac cgacgatacc ggtctgccca acgcggagtt tgtgccggcg agccagcatc
     5041 cgttgtccac catccgatga agccgctcga aggacagcat attcgccggc atcatcgatc
     5101 aggaaaatcc ctgtgtgata aaaattgaag cgctctgaga tcaggtggac tgtatggtcc
     5161 aaaaccctct ccagctcatg aatcgaagcc gattcactgg ccacatcagc agccacttga
     5221 agctggatcg cacggtgttg gagctcttga gtccgttcag cgacatgctg ctccaaattt
     5281 gtaattaatc cccgcatctg agcaatcata ctattcatac tctgcgccag gttgcccagc
     5341 tcgtcttcgc tttcgaccgg aaccgaacgg gaaaaattac ccccagcaat attgtcagcg
     5401 acattcgaca gcgtagcgat cgggtggagc gtatggcgga ttgaaatcca gaccaaaaag
     5461 cccatgatca gagcgccgcc gattgccagc ggccaggctg cccgatgaag cagcctcact
     5521 ggggcgagga gagatgcttc agattcttga gtgatgatgg tccagttgta ggaggtgctg
     5581 gaagcgacag cccgcatcac atcaccatct tcccgggtat atcgaaaagg ctccgcgctg
     5641 ccagcttgag gctcagcgat aaccgcctga ttactgatat ccgtcccgag gagggttggt
     5701 tcgggtggaa tttgcaggat tatccggttc gcctgatcaa ggaccagcgc ctcacccaaa
     5761 actggcgtca tctcgggagt gagctgggcc gaaagagcct ccaatgagat ggaaaataaa
     5821 cctaccccag tcagtggctg gccgggagca tggattggta atgcgataat caattctgaa
     5881 ccaggagtgg caggctcatc ccacacgtgc acggcggttt ccgcgccacc gattacctgt
     5941 tgataaccgg acagttcgga gatgctgcgg aaaggcgatg tgcccgagga tgccaccaga
     6001 ccgccgctga gatcggcagt tccggcataa ttgagccagg gatgggtatc cgacagagcc
     6061 tgaagtgcga gcgattgatc ggaggtgggt agtgatcgca aacctggctg gttcagaaca
     6121 tcctgatagg cgcgaatttc tccatccagc cactgctcaa gctgactgtt cagactcagg
     6181 ttgttggctt ccagccttgc cagggcaccc tgttcaattc gctggctgga ttgcatggtc
     6241 agaaaactga tcatgaatgc aataattgga aaagtcaccc acaggataag aatcgtcaaa
     6301 cgaacttgca gcggtccgcg ttggagaagg cgctcagagg ccaattgccg gaagctggca
     6361 tagctgaata caacagccag gagcaggtta gtgagcagca tcggcaggct gggttggagc
     6421 cggtttgcca gaccaacatt tagcaaaact ccgataatct gggcgatcag cagcaaacgg
     6481 gtcaggctgc gtgcgctggg gaatacctgc ttatcgaaaa acgacaggaa gacgagagtg
     6541 agaataagca gcgtacccgc cccagcaatc gatacaggcc agattatttg ggcgaatgat
     6601 ccctgggtat aggcaatcag cggcagaaat ccgccctgat aattaaccga ctcgaggctg
     6661 gataaccaga tactttgacc tgtcaccatg
//
LOCUS       DATMXK010000464         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_39623, whole genome shotgun sequence.
ACCESSION   DATMXK010000464 DATMXK010000000
VERSION     DATMXK010000464.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_39623"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..546)
                     /gene="nadE"
                     /locus_tag="VN363_08550"
     CDS             complement(<1..546)
                     /gene="nadE"
                     /locus_tag="VN363_08550"
                     /EC_number="6.3.1.5"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559204.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008795 - NAD+ synthase activity [Evidence
                     IEA];
                     GO_process: GO:0009435 - NAD biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+) synthase"
                     /protein_id="HWQ84601.1"
                     /translation="MTAAEFKLPRRDIEMLNIDDIDLAINPDVARKILTGFIRTELTR
                     AGFSRAVIGLSGGLDSAISCYLAAEALGSENVLAVRMPYKTSSSDSLEHAGQVIDALG
                     VRSLTVEITEMVDPLFACFPEANQVRRGNAMARARMIVLYDQSEAFHGLVVGTGNKTE
                     ILLGYSTLYGDSACAINPLGDL"
     gene            complement(527..1402)
                     /locus_tag="VN363_08555"
     CDS             complement(527..1402)
                     /locus_tag="VN363_08555"
                     /inference="COORDINATES: protein motif:HMM:NF012993.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006807 - nitrogen compound metabolic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitrilase-related carbon-nitrogen hydrolase"
                     /protein_id="HWQ84602.1"
                     /translation="MLLTLALAQINTRLGDVPANLEKHLQLASEARASGADLLVFPEL
                     SLTGYVLQDLATAVAHRPNSDDPIFRPLLEASREIDLVVGFVDEDPRNRFFIASAYLS
                     GGKVVHVHHKLYMATYGLFDEGRFFAWGDSVQAFDTRFGRLGILICEDFWHASPPYLL
                     WLDGAELLLFTSASPGRGLSKEPVLDSARWVEHLNQAYASMFTCFVAHANRVGYEDGL
                     NFWGGSTLFDPSGELLVQGKYHEEGVVLADVDLNQLHRVRARLPLLRDERTSLVQREL
                     GRILSVHLPNDRGGI"
     gene            complement(1436..>2019)
                     /locus_tag="VN363_08560"
     CDS             complement(1436..>2019)
                     /locus_tag="VN363_08560"
                     /EC_number="2.4.99.-"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0042158 - lipoprotein biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase family
                     protein"
                     /protein_id="HWQ84603.1"
                     /translation="LVGSLVGGRAAFIVVNWEYFRQHLLEIFQVYQGGLSWPGAMAGG
                     LVLVILYAWRLGKPTGELLWVLFPLLTSIVGVALLACWLDGCAYGSLARGNFGLLTVD
                     ELDHRALRIPTQLIGILAAVLWYAGLDSQKERFSRLAPAGWVSILGMALIMFGLALLR
                     VDPGLYAYGMRLDAWAALIFTAFALVGVLGSRR"
BASE COUNT          576 a          589 c          470 g          384 t
ORIGIN      
        1 cagatcgccg agtggattga tagcgcaggc tgagtcgcca tacagggtag agtaaccgag
       61 taatatctcg gtcttattgc cggtgccgac gaccagacca tgaaacgcct cggattggtc
      121 ataaagcacg atcattcgcg cccgcgccat cgcattaccg cgccgaacct ggttggcctc
      181 tgggaaacac gcaaagagtg ggtcgaccat ttctgttatc tcaaccgtca gcgaacgcac
      241 gcctaaagca tcgataactt gcccggcatg ctcaagggaa tcggacgagg aagtcttata
      301 tggcatccgc acagccagaa cattctctga tcccagagcc tctgctgcaa gataacagga
      361 tatcgctgaa tcaagtccgc ctgacaaacc aattactgcc cgcgaaaatc ccgctcgggt
      421 cagctcagtc cggataaacc ctgtgagaat cttacgggct acatctggat taatagccag
      481 atcaatgtca tcgatattca acatttcgat atctctcctg ggcagcttaa attccgccgc
      541 ggtcattggg cagatgcaca gaaagaatgc gacccagctc ccgctgcacc agtgaggtgc
      601 gctcatcccg caggagcggc aaacgtgctc gcacccgatg aagctggttc aggtcaacat
      661 ccgccaggac cacaccctct tcgtggtatt tgccctgaac taacaactca ccactgggat
      721 caaacagggt cgagccgccc caaaaattta gaccatcttc atagcctacc cggttggcat
      781 gagcgacaaa gcatgtgaac atactggcat aggcctggtt gagatgttcc acccaacgag
      841 ccgaatccaa cactggctcc ttactcaagc ctcgcccagg tgaagctgaa gtaaataaca
      901 acagctcggc accatccaac caaagcaaat atggaggtga agcatgccag aaatcctcac
      961 agatcaagat acccaaacgt ccaaaacgcg tatcaaatgc ctggaccgaa tcaccccagg
     1021 caaaaaaacg accctcgtca aataatccat aggtcgccat atagagttta tggtgcacat
     1081 gaacgacttt tccaccggag aggtatgcgc tggcaataaa gaaacgattc cgcggatctt
     1141 catccacaaa ccctacaacc agatcaatct cgcggctggc ttcaagaagg ggtcgaaata
     1201 tcggatcgtc tgagttcggc cggtgggcaa ctgccgtcgc caggtcctgg agcacgtagc
     1261 ccgtcaggga aagctccgga aatactaaca ggtcggcacc cgaagctcgc gcttcggagg
     1321 ctaactgcaa atgcttttcc aaattcgccg ggacatctcc caggcgagta ttgatttgag
     1381 cgagagccag agtcagaagc atggtgaagt ccactacctt tgaaatacga aatcgttaac
     1441 gccgggaacc cagtacccca accaaagcga atgcagtgaa gatcagggct gcccaggcat
     1501 cgagacgcat cccgtaagca tacaaacccg ggtcaacgcg cagcaatgcc aagccgaaca
     1561 tgatcagagc catcccgagg atactcaccc atccggctgg tgccaggcga gaaaaacgtt
     1621 ctttctgaga gtctaatcct gcataccaga gcacggcggc cagaattcct atcagctggg
     1681 ttgggatacg caatgcccgg tgatccagct catctacggt aagaagtccg aaatttccac
     1741 gtgccagcga gccataggca catccgtcca accaacaggc taataaagca acgccaacaa
     1801 tactggttaa taagggaaac aacacccaga gcaattctcc ggttggcttt cccaatcgcc
     1861 aggcatacaa tataaccaac accaaccctc ctgccatggc tccaggccag gatagtccgc
     1921 cttgatatac ctgaaatatc tccagaagat gctggcgaaa atattcccaa tttacaacaa
     1981 taaaggcagc tcttccccca accagactgc ccactaaag
//
LOCUS       DATMXK010000465         2635 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_112784, whole genome shotgun sequence.
ACCESSION   DATMXK010000465 DATMXK010000000
VERSION     DATMXK010000465.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2635)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2635)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2635
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112784"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            263..1480
                     /locus_tag="VN363_08565"
     CDS             263..1480
                     /locus_tag="VN363_08565"
                     /inference="COORDINATES: protein motif:HMM:NF024850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ84604.1"
                     /translation="MIKSKRVYLVLSIILALSFVLSACAQQAAPPAATEAPSEPAATE
                     APAADVLPDVIKIGVNETMTGWGAAYGDMTWKGIQLAHDTKPEVLGKPVELVLVDNKS
                     EKSEAALAAQRLVEVEKVAAVLGVNSSSMSMAQNEVLEAAKIPSMATAATNPLVTQGK
                     EYAFRACFIDPFQGKAAAKFATEELGAKTAVLLVDIALDYPVGLANYFRSNFIEMTGD
                     EKAILSYQSFQTGDRDFTAQLTQIKSLNPDIVFAPNEYAELGAILQQAKQLGITSKFL
                     AGDSADVPELVQIAGENAEGFMYTAQFHPDAFTHPEAGVFTEAFRAAYNIDPETFSVT
                     GYDGYMLLLDAIERAGSVDPEKIREALLATSFTGARGLTEFDADHNGILAVPVIEIVG
                     GKKTFRYLVSPGE"
     gene            1908..>2635
                     /locus_tag="VN363_08570"
     CDS             1908..>2635
                     /locus_tag="VN363_08570"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWQ84605.1"
                     /translation="MDPELFLQFLINGIALGSLYALIAIGYTIIYGVVQLINFAHGEF
                     FMVAAFMTMWGVSQYGLQWYVAFPLAGVLVVGLALVVDRSVYRPLREQKTSSFIGAIS
                     VSFLLQNLFVVLFTSRAKPFPHPAILDEVINFGTVALPVVNIFIVAVAILMFTGLFLL
                     VQYTDIGRAMRAISKDLEVSQLMGVNPNRVIAFAFIISALFAAVGAFMYCSKFPYVDP
                     FTGAIPGLKAFIGAVMGGIGSIPGA"
BASE COUNT          540 a          761 c          757 g          577 t
ORIGIN      
        1 accccagggg ttgaaatcct gcggatttct taaacccctg gggtctggtg attgccaacc
       61 cgtgggtctg gagactggcc gtcacctata gtgctcaacc gtccactttg cctggatgac
      121 atctatcccg ctatttcctg acattaactt tttctcattg tagtaaaatc agaatataaa
      181 atttgccctg gctggggggg agtatttcct cctctggcgg ggcaaggtca tatttgccca
      241 tatataaaag gagacggaag aaatgattaa aagcaagcgt gtgtatctcg ttctgagcat
      301 catcctcgca ctgtcattcg ttctatccgc ctgcgctcag caagccgccc cgcctgcggc
      361 aactgaagcg ccatccgaac cggcagccac cgaggcgcct gccgcggatg tactcccgga
      421 cgtgatcaag atcggggtca acgagaccat gaccggctgg ggagcggctt atggggatat
      481 gacctggaaa ggcatccagc tggcgcacga caccaagccc gaagtgctgg gcaagccggt
      541 tgagctggtg ctggtggaca ataagagtga aaagagtgaa gctgccctgg cagctcagcg
      601 cctggtggaa gttgaaaagg tcgctgcggt gctcggggtg aactccagct cgatgtccat
      661 ggcccagaat gaagtccttg aagcggccaa aattccttcg atggccaccg ccgccaccaa
      721 cccgctggtg acccagggca aggaatacgc cttccgggcc tgcttcatcg atcccttcca
      781 aggcaaggct gcggccaagt tcgccaccga agagctgggc gccaagaccg ctgtgctgct
      841 ggtcgatatc gccctggatt accccgtggg cctggcgaat tacttccgca gcaatttcat
      901 cgagatgacc ggtgatgaga aagccatcct gtcctatcag tccttccaga ccggcgaccg
      961 cgatttcacg gcccagctga cccagatcaa gtcgctgaat ccggatatcg tcttcgcccc
     1021 gaacgaatat gctgaattgg gcgccatcct gcagcaggcc aagcagctgg ggatcacctc
     1081 caaattcctg gcaggcgatt cggcggatgt gcctgagctg gtgcagattg ccggcgagaa
     1141 cgccgaaggt ttcatgtaca cggctcagtt ccacccggat gccttcaccc accctgaagc
     1201 cggtgtgttc accgaagcct tccgcgccgc atacaatatc gaccccgaga ccttctcggt
     1261 gaccggctat gatggctaca tgctcctgct ggatgctatc gagcgcgccg gatcggttga
     1321 tccggagaag atccgcgaag ccctcctggc aaccagcttc accggggcgc gcggactgac
     1381 cgagttcgac gccgatcaca acggcatcct ggcagtaccg gtgatcgaga tcgtgggcgg
     1441 taagaagacc ttccgctacc tggtttctcc aggcgagtaa gttcaactaa attctcaaag
     1501 ttggggtttt gcgagatgat cgcaaaaccc caactgctat ctattaattg tcattgcgag
     1561 cggagcctcc tgtcattgcg agcggagact cctgtcattg cgagcggaga ctcctgtcat
     1621 tgcgagcgga gcgaagcaat ctataattcg caggctcgag agtatgcctg gcaaagagca
     1681 gattgcttcg acaaagtgcg tctcgcaatg acagaaaatg gatgtcattg cgagcggagc
     1741 ctcctgtcat tgcgagcgga gactcctgtc attgcgagcg gcgctccctg tcattgcgag
     1801 cggagcgaag caatctataa ttcgcaggct caaaagtatg cctggcaaag agcagattgc
     1861 ttcgacaaag tgcgtctaaa caaaactaaa gagaaagtca ggtgtggatg gatcctgagt
     1921 tgttcttgca gttcttgatt aacggcatcg ccctgggcag cctgtacgcc ttgatcgcga
     1981 tcggctacac gatcatctac ggggtggtgc agctcatcaa cttcgcccac ggcgaattct
     2041 tcatggtggc agccttcatg accatgtggg gggtgagcca gtatggcctg cagtggtatg
     2101 tcgcctttcc cctcgccggg gtgctggtcg tcggattggc cctcgtggtg gaccgctcgg
     2161 tgtaccgccc gctgcgcgag cagaagacct cctcgttcat cggggcgatc agcgtctcct
     2221 ttctgcttca aaacctgttc gtggtcctgt tcacgtcgcg ggccaagccc ttcccccacc
     2281 cggccatcct ggacgaagtg atcaattttg gcacggtggc cctgccggtg gtgaatatct
     2341 ttatcgtggc cgtggccatc ctgatgttca ccgggctgtt cctgctggtg cagtacaccg
     2401 atatcgggcg ggcgatgcgg gcgatctcca aggatctgga agtctcgcag ctgatggggg
     2461 tgaatcccaa ccgggtgatc gccttcgcct tcatcatcag cgccctgttc gcggctgtcg
     2521 gcgccttcat gtactgctcc aaattcccct acgtcgatcc cttcaccggc gccatccccg
     2581 gcctgaaagc ttttatcggg gcggtgatgg gcgggattgg cagcatcccg ggggc
//
LOCUS       DATMXK010000466         3925 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_63827, whole genome shotgun sequence.
ACCESSION   DATMXK010000466 DATMXK010000000
VERSION     DATMXK010000466.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3925)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3925)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3925
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_63827"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..681
                     /locus_tag="VN363_08575"
     CDS             <1..681
                     /locus_tag="VN363_08575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84606.1"
                     /translation="GYANACGLAAAAPLTLELQNAFDEAILQAWKDSSVPPVLLKQMI
                     RYESQFWPGRWGEYHYGLGHMTYFGAHTTLYWRPALYQDICSLSGNCKGEIDYDEIMY
                     FLNLMDAYCPTCENKIDMAKAQKSVSYLAEALYAHCEQTTRIISNAAEIWPTAVVDYP
                     TLWKLTLMNYNVGPNCVFTSLSDAYDFAQSQVSWWDISYFTGDTQCQRGIYYANQITE
                     KFYDFLPD"
     gene            complement(769..1371)
                     /locus_tag="VN363_08580"
     CDS             complement(769..1371)
                     /locus_tag="VN363_08580"
                     /inference="COORDINATES: protein motif:HMM:NF024878.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HD domain-containing phosphohydrolase"
                     /protein_id="HWQ84607.1"
                     /translation="MLDYFPKSYPKETLDFIIRVAYLSEIKEWDCRPHIERIRKYSYI
                     IGSGTQIDRQTNEIISMASQLHDIGKAMMPDQLLHDGGIYDDTERPLIERHTIDGAKI
                     LEGSSSSVIQIGASIALTHHERWDGSGYPNHLKGEEIPLGGRICAIADVFDALTTARS
                     YKQTISKDEALQLIMDSSGVLFDPALVKAFKELYAEILKI"
     gene            complement(1498..2691)
                     /gene="galK"
                     /locus_tag="VN363_08585"
     CDS             complement(1498..2691)
                     /gene="galK"
                     /locus_tag="VN363_08585"
                     /EC_number="2.7.1.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00131.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004335 - galactokinase activity [Evidence
                     IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="galactokinase"
                     /protein_id="HWQ84608.1"
                     /translation="MHPRDFVRQTYIQRFGNEPDYLAMAPGRVNLLGEHVDYNDGFVL
                     PAAIDRATYVAFSAAEAGVEHSTILAADFGEVATFTRESIFSHTDAEGKPLSEWARYP
                     AGVAWALAEAGLAIPPMQAVFASDIPRGAGLSSSSSVELAFGTAWSALGGWSIPPMEL
                     AKLGQRAENLYVGVNCGIMDQFASACGVADKLLLLDCRSLEWQTLPIPENIAIVIADT
                     SVRRKLTSSEYNARRGACEEAVSILKQDLPSIRALRDVSVEDFNRYCRKLPKVVEGRA
                     RHVVEEVERTLRAIPLLQEGDVQAFGQIMNQCHVSLRDLYEVSIPELNIMVDIAQSLP
                     GCLGARLTGAGFGGCTVNLVEKEHAEAFAIELAASYTSKTNLKPEIYICRISDGAKVI
                     PPTLH"
     gene            complement(2752..>3925)
                     /locus_tag="VN363_08590"
     CDS             complement(2752..>3925)
                     /locus_tag="VN363_08590"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84609.1"
                     /translation="WVYDQGSQPQKALEYYRQAAAMPCDLCFPNRLEDILILKAAQIA
                     NPSDGRAAYYLGNFWYAHFQSEEAIACWEKAAGLDSTFATVQRNLGLAYFNKRNDHSK
                     AQAYFEKAFALNPCDGRVLFELDQLYKRLYTAPVNRLANLDRYPDLVAQRDDLTIERI
                     TLLNLLGRSGEAYEALMGRNFHPWEGGEGKVTGQYVTSLVEMAKAELVAGNGAQAVEL
                     LTRAQTYPQNLGEGKLYGALENNIFYYLGCAYETLGKLDKAKAALVQASRGFSEPSSP
                     RYYNDQPPDLIFYQGLALLKLHREKEAQAVFQKLVDYGTTHMSDEVGMDYFAVSLPDF
                     LVFDVDLNERNRLHCHYLLGLGYLGLEDKASARGHFEAALALDASHSGASLHMRMI"
BASE COUNT          941 a         1023 c         1006 g          955 t
ORIGIN      
        1 ggctacgcta acgcctgcgg gctggccgcc gcggctccgt tgacgctcga gctgcaaaat
       61 gcatttgatg aagcgatctt gcaggcctgg aaggacagca gcgttccacc agttttatta
      121 aagcaaatga tccgttacga aagccagttc tggcccgggc gctggggtga gtatcactat
      181 ggcctggggc atatgaccta ctttggcgct catacaacgc tctactggcg gccagcgctc
      241 tatcaggata tctgcagcct ctctggaaat tgtaaaggcg agattgatta cgacgaaatc
      301 atgtatttct tgaacctgat ggatgcttac tgcccgactt gcgagaataa gatcgatatg
      361 gccaaggcac aaaaaagtgt ctcatatctg gccgaagccc tttacgcgca ctgtgaacag
      421 accacccgaa tcatctctaa cgccgccgaa atatggccta cggctgtcgt agattacccc
      481 acgttgtgga aactgacctt gatgaattac aatgtcggtc ctaactgcgt atttacatca
      541 ttgagcgatg cgtatgattt tgcgcaaagt caggtttcgt ggtgggatat aagctacttt
      601 acgggggata ctcaatgcca acggggtatt tattacgcaa accagattac tgaaaaattt
      661 tacgatttcc tgcccgatta gactcgtttg gaataactcc gttgtaccag gataataaaa
      721 ccggtcagag atttctctcg accgattttc gttttcgggc aaactgggct aaattttaag
      781 gatttcagcg tacagctctt tgaaggcttt taccagtgcg ggatcaaaca ggaccccact
      841 cgagtccatg atgagctgta aagcttcatc cttactgatg gtttgcttgt aggatcgagc
      901 tgtggttagg gcatcaaaca catccgcgat cgcgcaaatc cttcccccaa gtggtatctc
      961 ctcacccttg agatggttgg gatatccgga gccatcccag cgttcatggt gggtgagagc
     1021 gatgcttgcg ccaatctgga taacggacga ggaggaacct tctaaaattt ttgcgccatc
     1081 gatcgtatgg cgctcgatca gtggtctctc tgtatcatca tagataccac catcgtggag
     1141 taactgatcg ggcatcatcg ctttcccaat atcgtgcagc tgggatgcca tggagatgat
     1201 ctcgttcgtt tgccggtcga tttgggttcc actaccaata atataggaat atttccggat
     1261 ccgctcgatg tgggggcgac aatcccattc cttgatctcg gataaatatg ccacacggat
     1321 aataaagtcg agggtttctt tgggatagct ttttggaaaa tagtccaaca ttgaaataac
     1381 ctcacggaca atgaccctgg aagacctctg ctacatggct gaggaacagg cagggaatcg
     1441 aatagagtag accaagaggg tagcaaaggg gaagttattt gtcaagctat gtttgcatta
     1501 gtgtagagta ggtgggatca ctttggcgcc atcagatatc cggcagatgt agatttcagg
     1561 cttgaggttc gtcttgctgg tatagcttgc agccagttct attgcaaagg cctcagcgtg
     1621 ttccttctcg acaagattta ccgtacagcc tccaaacccg gcaccggtca gccttgctcc
     1681 caggcacccg gggaggcttt gggcaatatc gaccatgata tttaattcag gaatagaaac
     1741 ttcatacagg tcacgcaaac tgacatgaca ctggttcatg atctgcccga aagcctgaac
     1801 atctccttct tgtaggagcg ggatggcacg cagggtgcgc tcaacttctt cgacgacatg
     1861 cctggcgcgc ccttcgacga ccttgggtag tttgcggcag tagcgattga aatcctccac
     1921 gctgacgtcg cgcaaagcgc gaattgacgg aaggtcctgt ttcaagatac tgactgcttc
     1981 ttcgcaggct ccgcgccggg cattatattc actgctggtc agcttgcgcc ggaccgatgt
     2041 atcggcaatg acgatcgcga tattttccgg aataggcagg gtctgccatt ccagcgaccg
     2101 gcagtcgagc aggagaagtt tatcggctac accgcaggcg gaggcaaatt gatccatgat
     2161 gccgcagttg acccccacgt atagattttc tgcccgctga ccgagttttg ccagctccat
     2221 cggtggaatc gaccagccgc ccagagctga ccaggctgtg ccaaaagcca gctctaccga
     2281 agatgaggaa cttaaccctg ccccgcgggg aatgtccgat gcaaatactg cttgcatcgg
     2341 tgggatagcc agacccgctt cggcgagcgc ccaggcaacc ccggcaggat agcgtgccca
     2401 ctcagagagc ggcttgccct ctgcgtctgt gtgggagaag atactttccc gcgtaaaagt
     2461 tgccacttcg ccaaaatccg ctgccaggat ggttgaatgt tccacgccgg cttcggctgc
     2521 agagaatgca acataagtcg cccgatcgat ggccgcgggc agaacgaacc cgtcgttata
     2581 atccacatgc tccccgagca aattgacccg cccgggtgcc atagccagat aatcaggttc
     2641 atttccaaac ctttggatat atgtctgccg gacgaagtct ctagggtgca taatttgtct
     2701 caattggtac ggttgttagt ctacaggaag cgggatgatg cgtttcctgc ctcagatcat
     2761 acgcatgtgc aggcttgctc ctgaatggct ggcatccagc gcgagtgcag cttcaaaatg
     2821 ccctctggcg gaggccttat cctccaaacc caggtaaccc agccccagca gataatgaca
     2881 atgcaggcgg ttgcgctcgt tcaaatccac atcgaagacc agaaaatccg gcaaagatac
     2941 ggcaaaataa tccatgccaa cttcatcgct catgtgggtc gtcccgtagt ctaccagctt
     3001 ctgaaatacg gcctgggctt ctttctctcg gtgtaatttc agcagcgcca gtccttgata
     3061 gaagatcaag tctggaggtt ggtcgttata atatcgaggc gagctcggtt cactgaatcc
     3121 gcgcgaggcc tgcacaagag cagccttagc cttgtccaac ttacccaaag tttcgtatgc
     3181 acagcctaaa taataaaaga tgttattttc cagggcaccg taaagcttgc cctcccccag
     3241 gttctgtggg taagtctggg cgcgggtcaa taactcaaca gcctgggcac cattgccggc
     3301 aaccagctcg gctttagcca tttccaccag actggtcaca tactgaccgg ttactttccc
     3361 ctcaccgccc tcccaggggt gaaaatttct tcccatcaag gcttcgtaag cttcaccgga
     3421 gcgtcccagc aggttgagca atgtgattcg ctcgatggtt aaatcatctc gctgtgccac
     3481 aagatccgga tatcgatcca gatttgccag gcggttcacc ggtgcggtat aaagccgttt
     3541 gtacagctgg tcaagctcga ataaaacccg accgtcgcaa ggattcaatg caaaggcttt
     3601 ttcaaaataa gcctgcgctt tagagtgatc atttcgctta ttgaaataag ccaaccccag
     3661 attacgttgg actgtggcga acgtgctgtc gagacccgca gccttttccc agcaggcaat
     3721 cgcttcttcg gattggaagt gcgcgtacca gaaattaccc agatagtaag ccgcgcgccc
     3781 atcggatgga ttggcgattt gagctgcttt caggatcaag atgtcttcca gccggttagg
     3841 gaaacacaaa tcgcagggca tggctgcggc ttgccggtaa tattcaagcg ctttctgcgg
     3901 ctggctgcct tgatcgtata cccag
//
LOCUS       DATMXK010000467         2728 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_143326, whole genome shotgun sequence.
ACCESSION   DATMXK010000467 DATMXK010000000
VERSION     DATMXK010000467.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2728)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2728)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2728
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_143326"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1684)
                     /locus_tag="VN363_08595"
     CDS             complement(<1..1684)
                     /locus_tag="VN363_08595"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84610.1"
                     /translation="MITHARARACIHLGEGSLDPRQVEQLASHLSRCRACREYAALQE
                     QLAHAITHQMHTHWDARIENYRPAPMPEISRSKTMSFKRALAFTAVVLALGVVVLGAP
                     PLLRRALPSALTQPTVPLSTPAVTELPAISPNPTDEISDLPLQLDPAICVDQSRPAPE
                     GVNLPYPASKVIGGGKVQNGDFTFYLWLYCDEKLSPQDMEHFSDLGGLGTYARWMYTG
                     PKIEGNFWDMVGIEPDVRNITGSPMLSNSGASMGMGLNLPDEPAGKWEVAELARSGEP
                     IRFAVKVQSIDGVYGAELAFRLKAGKDGYIPDGIAVQKLAVEPLSDLPAVELPEAQKL
                     PPVGTPIVHYPAANGLIAVSSGSSVQGGQLDIFTLQADGSGLTNLTKIPAADTYPVWS
                     PDGSQIAFLSDRRSAGIYDIYVMNADGTQIKPVFLSPYPIVKLDDRGQTVLPSMLWLN
                     WSPDGQYILAEASQADGKEKCLVLARADGTDGRCINNLAFEAPQWSPDGKYISYNKQA
                     TYAGIARLDVQALFTQDSRLESWIYRLPNWIPEGQAQWSPDSQLVASIVVNEA"
     gene            complement(1681..2244)
                     /locus_tag="VN363_08600"
     CDS             complement(1681..2244)
                     /locus_tag="VN363_08600"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA polymerase sigma factor"
                     /protein_id="HWQ84611.1"
                     /translation="MDHSELALLLSNCQAGDPQAIASFYAAYHLDLYRLALSILDDPG
                     EADEAAQEALLRAIDRLPSYRGEAGFKSWLYAITVNVCRGRLRKQRVRQRLQQTLFGL
                     FGQGETRPIHPEEQVIQNELDSALWQSIRALPEALRLTITLRYYHALPIGEIAQVMQV
                     SQRTVHNRLSDAHSRLRAALEEKVVTK"
     gene            complement(2338..>2728)
                     /locus_tag="VN363_08605"
     CDS             complement(2338..>2728)
                     /locus_tag="VN363_08605"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84612.1"
                     /translation="CTAAPTANPTLAPVASTPQPQVTSTPADIYEISAWVDIPEPGPD
                     DIVTVSGHLIRNEAYLGGVGMWMFWPDEEGGPVMHECVSYMMYQRGICQIIVKDYPRG
                     KYVPVVVRVEFQDRIFTTETGFTPREK"
BASE COUNT          597 a          807 c          767 g          557 t
ORIGIN      
        1 tggcttcatt gactacaata ctcgcgacca gctggctgtc cggcgaccac tgcgcctggc
       61 cttcaggtat ccaatttggc agacgataga tccaggattc cagccggctg tcctgggtga
      121 acaaagcctg cacatccagc ctggcaatcc cggcataggt ggcttgcttg ttatatgata
      181 tgtacttacc atccggcgac cactgcggcg cttcaaacgc caggttattg atacacctgc
      241 cgtcagtgcc atccgctcgc gccagaacca ggcatttctc cttgccatca gcctggctgg
      301 cttcggccag gatatactgc ccatccggtg accagttgag ccacagcatt gaggggagaa
      361 cagtctgacc ccgatcatcg agctttacaa tcggatatgg cgagagaaat accggcttaa
      421 tttgcgttcc atcggcgttc atcacgtaga tatcataaat ccctgcgctg cgccggtcgg
      481 agaggaaagc gatttgtgat ccatcgggtg accacacggg ataagtatct gcggcaggaa
      541 tcttcgtaag attggtcaaa ccgctgccat cagcctgcag tgtaaagatg tccagctgcc
      601 ctccctggac tgaggagccg ctggatacgg cgatcagccc attagcagcc ggatagtgta
      661 cgattggtgt accaacaggc ggtagttttt gggcttctgg caactctact gccggcagat
      721 cggacagggg ctcgaccgca agtttctgca ccgcaatccc atcgggaata tatccgtcct
      781 tgcccgcttt gagccggaaa gccagctctg ccccatacac gccatcgata gactggactt
      841 tgacggcgaa acggatgggt tctccactgc gggcaagctc ggccacttcc catttaccgg
      901 caggctcatc gggcagattc aatcccatcc ccatgctggc gcccgaattt gagagcatcg
      961 gcgatccagt gatattccgc acgtccggct cgatccccac catatcccaa aaattgccct
     1021 cgattttcgg tccggtatac atccagcgag catacgtacc cagcccgccc aggtcgctga
     1081 aatgctccat atcctgcggg ctgagtttct cgtcgcagta cagccagaga tagaaggtaa
     1141 aatctccgtt ctgcactttg ccacccccaa tcaccttgct cgccgggtat ggcaggttga
     1201 cgccctccgg ggcaggccgg ctctggtcca cgcaaatcgc agggtcaagc tggagcggca
     1261 gatcactgat ttcatcggtt ggattagggc tgatagcagg cagctctgtc acggcaggtg
     1321 tggaaagcgg cacagtcggt tgagtaagcg cggagggcag ggcgcggcgc agcaacggcg
     1381 gcgcccccag gaccaccaca cccagggcca ggacaacggc ggtaaaggcg agagctcgtt
     1441 tgaacgacat cgttttgctc cttgatattt caggcatcgg cgccgggcga taattttcga
     1501 tccgggcatc ccagtgggta tgcatctggt gggtgatcgc atgcgcaagc tgttcctgga
     1561 gggcggcgta ctcacggcag gcacggcagc ggctcagatg gctggcaagc tgctcgacct
     1621 ggcgagggtc aagcgatccc tccccaaggt gaatacaggc acgggctcgg gcatgggtta
     1681 tcatttcgtc accacctttt cttccagcgc tgcccgcaag cggctgtggg cgtcactcag
     1741 ccggttgtgg accgtgcgct ggctgacctg catgacctgg gcaatctcgc cgatcggcag
     1801 ggcatgataa tagcgcagcg tgatcgtcag gcgcaaagcc tccggcaggg cgcggatgga
     1861 ctgccacagc gctgagtcga gctcattctg gatcacctgc tcctcgggat ggatcgggcg
     1921 ggtttcaccc tgcccaaaca ggccaaacag agtctgctgc aagcgctggc gcacgcgctg
     1981 tttgcgcagg cgaccgcggc acacattgac cgtgatggcg taaagccagc ttttaaaacc
     2041 cgcctcgccc cggtaagaag gcaaacgatc gatcgcacgc aagagcgcct cctgggcagc
     2101 ctcatcggcc tcgcccggat catccaggat ggacagcgcc aggcggtaga gatccaggtg
     2161 ataggccgcg taaaatgagg cgatagcctg gggatctcca gcctggcagt tcgagagcag
     2221 cagtgcaagt tctgagtgat ccatctatcc tttaagacgc gcttcgggcc aaaaacctga
     2281 aataaaaacc gctccccaat gagaagcggt tcccagaacc agttatattc taaccgttta
     2341 cttttcccgg ggtgtaaagc ctgtctcggt ggtaaaaatt cgatcctgaa actcaacccg
     2401 gaccacgact ggaacatact tcccacgggg gtaatccttg acaataatct ggcagatacc
     2461 gcgctgatac atcatataac ttacacactc atgcataacc ggcccgcctt cttcatccgg
     2521 ccaaaacatc cacatcccga caccccccag gtaagcttca ttgcggatca gatgaccact
     2581 gacggtcaca atatcatcgg gaccaggctc ggggatatcc acccaggcag aaatttcata
     2641 gatatctgcc ggggtggaag tgacctgggg ttgtggagtg ctcgcgacag gcgccagcgt
     2701 tggattagcg gttggagcag ccgtgcac
//
LOCUS       DATMXK010000468         2045 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_95284, whole genome shotgun sequence.
ACCESSION   DATMXK010000468 DATMXK010000000
VERSION     DATMXK010000468.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2045)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2045)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2045
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_95284"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..188
                     /locus_tag="VN363_08610"
     CDS             <1..188
                     /locus_tag="VN363_08610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560183.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWQ84613.1"
                     /translation="NTVVRQTGNIGLDSWEPIVQIAVGSEPQVSYGKVTPFAATRIME
                     EHVVGGKVVRDYLIEQV"
     gene            191..1978
                     /locus_tag="VN363_08615"
     CDS             191..1978
                     /locus_tag="VN363_08615"
                     /EC_number="7.1.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560184.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0010181 - FMN binding [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoF"
                     /protein_id="HWQ84614.1"
                     /translation="MAYFRSHVLVSVDPECLVRGAHDVLDALNDELVSQGLIDEIQVL
                     ETSRIGDPVKFGPDMMVYPEGVHYASLTADEIPYLVEEHFLKGRVVEKMRAHEAAAVD
                     EELGPPRSKEIRVVLRNCGKIDPTNIEDYIAEDGYMALGKVLSELTPEQVIETVLASG
                     LRGRGGAGFPAAKKWQLCRSNPGDIKYVICNADEGDPGAFMNRRVLESDPHAVIEGMA
                     IAAYAIGSCQGYIYCRAEYPLAVQTLEIGIQQARSLGLLGNNIMGSDFSFDLEVRMGA
                     GAFVCGEETALMASIEGKRGEPRPRPPFPAARGLWQKPSNINNVETYANVPQIILKGP
                     EWFASMGTEGSKGTKTFAIAGDVTNTGLIEVPMGITLREIVEDIGGGIKDGKALKAIQ
                     TGGPMGGCLPASAMDLKVDYESLTRAGSMMGSGGMIIMDEDTCMVDIARFFMEFTQDE
                     SCGKCTPCRIGTRRILEILEAICEGRGQAGDIERLETLCFEVQRNSLCGLGQGAPNPV
                     ITTLKHFRAEYEAHIYEKRCPAKVCRSLISYRIVAGICTGCTVCARNCPVGAISGERR
                     QTHLIDSDTCIRCGICQQVCNFNAIEILS"
BASE COUNT          445 a          562 c          620 g          418 t
ORIGIN      
        1 ccaatacggt tgtccgccag accggcaata tcggcctgga ttcctgggag cccatcgtac
       61 agatcgcagt gggcagtgag ccgcaagttt cgtacggcaa agtgacccct tttgcagcca
      121 ctcgcatcat ggaagaacac gtggtcggcg gtaaggttgt ccgggattat ctcatcgaac
      181 aggtgtaagg atggcttatt ttcgctcgca tgtattggta tctgtcgacc ccgaatgtct
      241 ggtgcgcggc gcgcatgatg tcctcgatgc attgaacgat gagctggttt cccaggggct
      301 gatcgatgaa atccaggtcc tggagacttc ccgcatcggc gatccggtta aattcggccc
      361 cgatatgatg gtgtatccag agggggtcca ttacgccagc ctgaccgccg acgaaattcc
      421 ctatctggtg gaagaacact tcctcaaggg ccgcgtcgtc gagaaaatgc gcgcccacga
      481 ggcggctgcg gtggatgaag agctgggtcc acccaggtca aaggaaatcc gggtcgtact
      541 gcgcaactgc ggcaagatcg atcccaccaa catcgaagac tatatcgccg aagatggcta
      601 tatggccctg ggcaaagtgc tcagcgagtt gacccccgaa caggtgattg agactgtcct
      661 ggcttccggg ctgcgcggcc gtgggggcgc cggctttccg gcggctaaga aatggcagct
      721 ctgccggagc aatccaggcg atatcaagta tgtgatttgc aacgccgatg aaggcgatcc
      781 aggggcgttc atgaaccggc gggtattgga atcggacccc cacgcggtaa tcgaaggtat
      841 ggcgatcgcc gcgtatgcga tcggttcctg ccagggatat atttactgcc gggcggagta
      901 ccccctggct gtccagaccc tggagatcgg catccagcag gcccgcagcc tgggactgct
      961 gggtaataat ataatgggat cggatttcag cttcgacctg gaagtccgca tgggggccgg
     1021 cgcgtttgta tgcggcgagg aaaccgccct gatggcttcc atcgagggca aacgcggcga
     1081 gccgcgcccc cgcccaccat tcccggcagc ccgcggtctg tggcagaagc cgtccaatat
     1141 taataacgtg gaaacctatg ccaatgtccc ccagatcatt ctgaaaggcc cggaatggtt
     1201 cgccagcatg ggcaccgagg gcagcaaagg caccaagact ttcgccatcg ccggggatgt
     1261 aaccaacacc gggctgatcg aagttccgat ggggatcact ttacgcgaga ttgtcgaaga
     1321 tatcggcggt gggatcaagg atggtaaggc gctcaaagcc atccagaccg gcggcccgat
     1381 gggcggctgc ctgccggctt cagccatgga tcttaaagtc gattatgaat cgctcacccg
     1441 ggccggctcg atgatgggct caggcgggat gatcatcatg gatgaagata cctgcatggt
     1501 ggatatcgcc cgctttttca tggaatttac ccaggacgag agctgcggta aatgcactcc
     1561 ctgccggatc ggtacccggc gcattctgga gatcctggaa gcgatctgtg aggggcgcgg
     1621 gcaggctggc gatatcgaac gcctggaaac tttatgtttc gaagtgcagc gcaacagcct
     1681 gtgcggactg ggtcaggggg cgcctaaccc ggtcattacg accctcaaac atttccgcgc
     1741 tgaatatgag gcccacatct acgaaaaacg ctgtccggca aaggtttgcc gctcgctgat
     1801 cagctaccgg attgtggctg gcatctgtac cggctgcacc gtctgtgccc gaaactgccc
     1861 ggttggcgca atctctggcg agcgccgcca gacccatttg attgactcgg atacctgcat
     1921 ccgctgcggg atctgccagc aggtctgtaa tttcaatgcg atcgagattc tctcctgagc
     1981 atgccagttt tgaagtgaat ggagtcagta tgactgaaag ccagatcaaa acaaatatcc
     2041 aggat
//
LOCUS       DATMXK010000469         3110 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_153138, whole genome shotgun sequence.
ACCESSION   DATMXK010000469 DATMXK010000000
VERSION     DATMXK010000469.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3110)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3110)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3110
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_153138"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..2109)
                     /locus_tag="VN363_08620"
     CDS             complement(<1..2109)
                     /locus_tag="VN363_08620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84615.1"
                     /translation="MFEPPDELSSDLEWMLESGQVDRDLLAEALVEEAAIELDRLAHL
                     YLGSAALARQVMVRALGAALVNVYRYRHGLGVRIWLMRFLVVEWRKTRPVQDSSLTDD
                     WTAVDHQSPRLRELGVLVYGGGFSLQNLALILETDVSNIERLLKDFNLQYFLETGLKS
                     GKKEANQAALDERLRRAAWQRGAAWNAPAPDLAALTNAVVAHATRSGARRGGLLRAQE
                     LGLVAIVIALAGAVIWGSGWLMAGEEAGPEPRVTLQTVIVEITTTPQPLAAGGGIPQV
                     APTPMRTAEASHPLSLESDSETIRRRIERAIDFRRSLWLDASLRFYYPYSREGVPGEY
                     RAQIWLDRWREHFLVLSGRQGEPIQEIYLYLLEEGTYQAQRSEHGEYDFQELAEGDGL
                     QTVYLAYQSLYLPMLRYTESDHYIPQELTRLLDRSVLVVDQVNLAGQRLATLWLDVNN
                     GLALRQRHYNPHSQSPLLDVDLDGLLINHRLPEQVFMPQFGWRGAYPMNDLLQFETEI
                     GETDPAAAIGEQLLPPAGFDPSSQALHFEFETIYMESIPDVLKDFLYHRASLFTEEYF
                     LGKVDFADPWQVICTRSSDGSRLAFAETGGESQSLYWFNLSDLALQSHNANGSVTQLA
                     FSPDGQRLAYFNAGQPLGELQVLDLQSGAEQTLLTLGDVRSLVWNPDGKLLGFIGREI
                     TPETDDRVMVVDVQTGYVVYE"
     gene            2222..2953
                     /locus_tag="VN363_08625"
     CDS             2222..2953
                     /locus_tag="VN363_08625"
                     /EC_number="3.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF021592.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070007 - glutamic-type endopeptidase
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="YhfC family glutamic-type intramembrane
                     protease"
                     /protein_id="HWQ84616.1"
                     /translation="MDLLAFTHAFNFVLMFLLAFGVGALITYKYRLGWRLFWIGAATF
                     ILSQVGHIPFNAGLTQLFKSGILPTPPAEWQLPFNAVGLGLSAGLWEELTRYAVYRWW
                     ARDARSWRKGLLLGAGHGGIEAAFVGILVLLTFINMLAARNIDLTDFYTGEELVIAVQ
                     QVNTYWGSAWYEPLAGALERAFSIPAQIALSILVLQVFTRKQIRWLWAAIAWHAVIDA
                     VAVYLSATYGVWVTEAAIGVTALLN"
BASE COUNT          695 a          915 c          816 g          684 t
ORIGIN      
        1 ttcataaacg acgtacccgg tctggacatc aactaccatc acccgatcgt ccgtctcagg
       61 agtgatttcc cgcccaataa atccaagcag ttttccatcc ggattccaaa ccaggctgcg
      121 gacatcaccc agggtgagca aagtctgttc ggcgccgctc tggagatcca aaacctgcag
      181 ctcgcccagc ggttggccgg cattgaaata ggccaggcgc tggccatctg gggagaaagc
      241 cagctgggta acactgccgt ttgcgttgtg agattggagg gccaggtcac tcaggttaaa
      301 ccagtacaag gattggcttt caccaccggt ttccgcgaaa gccagcctgc tgccatctga
      361 ggagcgcgtg cagattacct gccagggatc ggcgaaatcc actttaccca ggaagtattc
      421 ttctgtaaaa aggctggccc tatggtatag aaaatctttg agtacatcag gtatgctttc
      481 catataaatc gtttcaaact caaaatgcag cgcctgagag ctcggatcaa agccagccgg
      541 cggaagcagt tgttcaccta ttgcagctgc aggatcagtt tccccgatct cggtctcgaa
      601 ctgcagcaga tcattcatgg gataagcgcc acgccagcca aattgcggca tgaagacttg
      661 ctcgggtaaa cgatgattga tcaaaagccc atctaagtcc acatctagga gcggggactg
      721 gctgtgcgga ttgtaatggc gctggcgcag cgccaggcca ttgttcacat ccagccacag
      781 ggttgccaga cgctgccccg ccagattaac ctgatccaca accaacacag atcgatccaa
      841 taggcgagtc aattcctggg gaatgtaatg atcactttct gtgtaacgca gcatgggcag
      901 atacaaggac tgataagcaa gataaacagt ctgcaaccca tcaccctcag ctagctcttg
      961 aaaatcatac tcaccatgct cggagcgttg ggcctgatag gtcccctctt ctaataaata
     1021 gagatagatc tcctggatag gttcaccctg tctgccactc aaaaccagga aatgctcgcg
     1081 ccaacgatct aaccagatct gggcacggta ctcaccaggc acgccctccc gactgtaggg
     1141 ataataaaat cgtaacgaag catccaacca aagactgcga cggaaatcga tggcgcgctc
     1201 gatccgcctg cggatggtct ccgaatcgga ttccaggctg agcggatgag aggcttctgc
     1261 agtccgcatg ggggttggag ccacttgcgg aatcccgcca ccggctgcca gcggctgggg
     1321 agtggtcgtg atctctacga tcacggtctg cagtgtgacc ctaggctctg gaccagcttc
     1381 ctcccccgcc attaaccaac cactgcccca gattaccgct cctgccaggg caatgacgat
     1441 cgcgaccaac cccagctcct gggcacgcag cagcccgccg cgacgggcgc cgctgcgggt
     1501 ggcatgcgcc accaccgcat tggtcagggc tgccaggtca ggcgctggag cgttccaggc
     1561 tgcaccccgc tgccaggctg cgcggcgcag gcgctcatcg agcgccgcct gattcgcctc
     1621 ctttttaccc gatttcaggc cggtttccag aaaatactgc aggttgaagt ctttaagcag
     1681 gcgctcaata ttcgatacat ccgtttcaag aatcaatgca agattttgca gactgaatcc
     1741 acccccatag accagcactc ccagctcgcg cagacgaggt gattggtggt ccacggcagt
     1801 ccaatcatcg gttaagctgc tgtcctggac aggcctggtt tttcgccatt cgacgaccag
     1861 aaagcgcatc agccagattc tgacccccag accatgccgg tagcggtata cgtttaccag
     1921 cgccgcgccc agcgccctca ccatgacctg gcgcgccagt gccgcgcttc ccagatacag
     1981 gtgcgccagc cggtctaact caattgcagc ttcttccacc agggcttctg ccaggagatc
     2041 gcggtcaacc tgcccacttt ccagcatcca ttccaggtcg gatgatagct cgtcaggtgg
     2101 ctcaaacata tcggatcgcc cggataagtt ggaccattgc actccagtct agtcagattc
     2161 taccagtttc atacttcatg tataattaaa attcgatgat taagcccgat taaggataac
     2221 catggatcta ctcgccttca cccacgcctt caattttgtc ctgatgtttc tcctggcgtt
     2281 cggcgtggga gcactcataa cgtacaaata caggctgggc tggcggctct tctggattgg
     2341 cgcagcaacg ttcatcctct cccaggttgg gcacattccg ttcaatgccg ggttgaccca
     2401 gttatttaag agcggcatcc tgccaacgcc accggcggaa tggcagctgc catttaatgc
     2461 cgtcggattg gggctgagcg ccgggctctg ggaagaatta acccgctatg cagtttatcg
     2521 ctggtgggct agagacgccc gctcgtggcg caaggggctg cttctaggcg ccgggcacgg
     2581 cggcatcgaa gctgcgtttg tcggcatcct cgttttgctg acctttatca atatgctggc
     2641 agcccggaat atcgacctga ccgattttta caccggcgag gagttggtga ttgctgtgca
     2701 gcaggtcaat acttactggg gatcagcctg gtacgagccg cttgccggag cgctggaaag
     2761 ggctttttcc atcccggccc aaatagcact gtctatcctg gtcctccagg tgtttacccg
     2821 caagcaaatc cgctggttgt gggctgcgat cgcctggcat gctgtgattg atgcagtggc
     2881 agtctatctc agcgctactt acggcgtatg ggttactgaa gctgccatcg gtgtgacagc
     2941 tctgctcaac tagatcctga tctggcgctt acgccagcct gagccagctg aaccgctcat
     3001 agaatccgtc ccggtcccgg ctcccctcac ggctgctgat ctgaagttga aacctgccga
     3061 agaaaatccc gtaaatctag atgattctcg cttcagcgaa taaattggtg
//
LOCUS       DATMXK010000470         2365 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_15926, whole genome shotgun sequence.
ACCESSION   DATMXK010000470 DATMXK010000000
VERSION     DATMXK010000470.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2365)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2365)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2365
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_15926"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..820
                     /gene="amrB"
                     /locus_tag="VN363_08630"
     CDS             <1..820
                     /gene="amrB"
                     /locus_tag="VN363_08630"
                     /inference="COORDINATES: protein motif:HMM:TIGR04336.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="AmmeMemoRadiSam system protein B"
                     /protein_id="HWQ84617.1"
                     /translation="SPIAGRWYPDDPQELTESVDFYINQARLPQIHGEVIAIIAPHAG
                     HRYSGPVAGYAFAAVRSLKPEVVAVVSPMHYPYAEAVLTSAHTAYSTPLGEIALDQKA
                     LHDLDTSFTLANAEPIERVGRDPEHSLEIELPFLQRALSEKFTLLPLMVRELNPQPLE
                     ILGKHLAHALHNRPALLVASTDLSHFYPQAAAEKLDREMLRRIETFDPQAVLRAEDEG
                     AGFACGRGAVAVVLWAARELGANHVQILHYATSGDVTGDFHQVVGYGAVLITRQ"
     gene            846..2213
                     /locus_tag="VN363_08635"
     CDS             846..2213
                     /locus_tag="VN363_08635"
                     /inference="COORDINATES: protein motif:HMM:NF013741.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="hemolysin family protein"
                     /protein_id="HWQ84618.1"
                     /translation="MPEATLGSDVLRLLAVGLLVLANGFFVAAEFSLVSVRRTRIAEL
                     VAHGNDSAEAVQKAIQNPDRVIAATQLGITLASLGLGWIGEPALAHIIQPVVALFPNA
                     VQPNISHSISAGVAFVLITFLHVVVGELAPKSIALQDPEKTSLFIARPTLLVETIFKP
                     AIWALNGTGNFLLKLVGVNPASGHELVHSIAELKMLVTASTESGVMEQEESEMLHAVF
                     DFSDLVVRQVMMPRTEIEAVEADTPLIDIISLTTQSTYTKFPVYEENLDQILGIVHVK
                     DLLPALQGEDCTACVARDFTREPIFVPDSLPVDALLRTFRAQHQHIAIVIDEFGGTAG
                     LVTLEDLLEEIVGEVSDPFDAFVPEIEPLPDGSFRIDGLTLIEVVNAQLGLHLVDPDY
                     DTIAGYVLGKLSHLPRSNETIQADGVRLQVESMDGMRISQLLLTRLPAPLAPESAGHT
                     PPANA"
BASE COUNT          457 a          702 c          634 g          572 t
ORIGIN      
        1 ttccccgatc gccggtaggt ggtatcctga tgacccccag gaattgaccg aaagcgtcga
       61 tttttatatt aaccaggctc gcctgcctca aattcacggc gaagtgatcg ccatcatcgc
      121 ccctcacgcc gggcaccgct attcaggccc ggttgccggc tatgccttcg ctgcggtgcg
      181 cagcctcaag ccagaggtcg ttgctgtcgt ttcccccatg cattatcctt atgccgaggc
      241 ggtcctcacc tctgcccata ctgcttacag cacgccgttg ggagagattg ccctcgacca
      301 gaaagctttg catgatcttg ataccagctt caccctggct aacgctgagc caattgagcg
      361 ggtggggcgc gacccggaac attcgctcga aattgagctt ccctttctgc agcgcgccct
      421 ctctgagaaa tttaccctgc tccccctgat ggtgcgcgag cttaaccccc aacccctgga
      481 aatcctgggc aaacatctgg cccatgctct ccacaaccgc ccggctttac ttgttgccag
      541 taccgatcta tcccacttct accctcaggc ggctgccgaa aagctggacc gggaaatgct
      601 ccgccgtatt gaaacctttg accctcaggc agtgctgcgc gccgaagacg agggcgcggg
      661 attcgcctgt gggcgcggtg cagtggctgt tgttctgtgg gctgcgcgtg agttaggcgc
      721 caatcacgtc caaatcttac attatgcgac ttcgggtgat gtgaccggag attttcatca
      781 ggtcgttgga tacggcgcgg tgctgatcac ccgccagtaa tctgaaaggt cgaacaagac
      841 ccacgatgcc cgaagctacc ttaggttctg atgtcctccg tctgctggca gttggattgc
      901 tggttcttgc caacggattc tttgttgcgg ctgagttttc gttggtcagc gtccgccgca
      961 cccgcatcgc cgagctggtt gcccacggga acgattccgc cgaagcggtc cagaaggcca
     1021 tccaaaatcc agatcgggtc attgctgcca cccagcttgg catcaccctc gccagcctgg
     1081 gtctgggttg gatcggcgag ccagccctgg cgcatattat tcagccagtg gtggcgctct
     1141 tcccgaatgc tgtccaaccg aatatttccc acagtatttc tgcgggggtt gcctttgtgt
     1201 tgatcacatt cctccatgtg gtggttggcg agctggctcc caaatcgatc gccctgcagg
     1261 accccgagaa gacttccctg ttcattgcca ggcctacgct cctggtggaa acgatcttta
     1321 agccagccat ctgggcttta aatgggactg gcaatttcct gctcaaactg gttggggtaa
     1381 atcctgcctc ggggcacgaa ctggttcatt cgattgcgga attaaaaatg ctggtgactg
     1441 ccagcaccga atccggcgtc atggagcaag aagaaagcga gatgctgcat gcggttttcg
     1501 atttcagtga tctcgtagtt cgccaggtga tgatgccgcg caccgaaatc gaagctgtag
     1561 aagccgatac tcccttaatc gacattatct ctttgacgac ccaatcaact tatactaaat
     1621 tcccggtcta cgaagaaaac ctggaccaga tcctgggaat tgtgcatgtg aaagacttgc
     1681 tgcccgcttt gcagggtgag gattgtacag cctgtgtggc acgtgatttc acccgtgagc
     1741 ctatctttgt ccccgattcc ttacccgttg atgccctgct gcgtaccttc cgcgcccagc
     1801 accagcatat tgccattgtg atcgatgaat ttggcggcac tgccggactg gtcaccctgg
     1861 aagacctgct cgaagaaatt gttggggagg taagcgatcc tttcgacgcg ttcgtgcccg
     1921 agattgagcc tctgccggat ggctctttcc gaatcgatgg gctgaccctg atcgaagtgg
     1981 tcaacgcgca gttaggtctg catctggtcg atcccgatta cgacaccatt gcgggatatg
     2041 ttctcggcaa gctcagccac ctgccccgtt ccaatgaaac catccaggcc gatggggtgc
     2101 gtttgcaggt agagagcatg gatggcatgc ggatttccca gctgttgctt acgcgcctgc
     2161 ctgctccgct ggcgccggaa tctgccggac ataccccacc tgccaatgcc tgagttccag
     2221 ttctaatacc gccaatgcct gtatttgtag agatcgctgt caacgtacct caggtcagtg
     2281 gcgttttcca ttaccacctg ccgcccgagc tggaagggca gacccggccg ggccagctgg
     2341 taatcgtccc cttcggttct ttgtt
//
LOCUS       DATMXK010000471         3683 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_158232, whole genome shotgun sequence.
ACCESSION   DATMXK010000471 DATMXK010000000
VERSION     DATMXK010000471.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3683)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3683)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3683
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158232"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            125..2449
                     /locus_tag="VN363_08640"
     CDS             125..2449
                     /locus_tag="VN363_08640"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWQ84619.1"
                     /translation="MTLLPASSDRALSIYLALVQYPILSTQIRSRMRRELFRRGVITP
                     QGFEAEVRERAVRTQAMEGMHDPFAEEPTDVWETRLMHVRDHLTDFYFAYNLPYDLFE
                     KIVLETLAERGANSEEMLVSFNPELAPQTMLFEQALAIEKLAPEQRAAADARLQEIKV
                     VLIRTLISDQLAYLKIAKDWFNVADLIEIRNRKIGQGKIGGKAAGMLLAARILIEVGG
                     EDIREHLRIPESYFIGADLTYSFMSMNGLLYWGDQKYKTEAQIRADYPQLQQEFIAGQ
                     LPQDVVERLGELLEEVKSKPLIVRSSSLLEDNFGTSFAGKYESHFCPNQGTPTQNLED
                     LTRAISSIYASILNPDALLYRRARGLQDYDERMAILIQVVEGQQSGRYFLPHAAGVAF
                     SRNLYRWSPQIRREDGFMRLVWGLGTRAVDRVGNDYPRLVALSHPLLHPEASTKAIRR
                     YSQRYVDVIDMEANAFKTLPVGEVLGSHYPPLRYLVQVDQGGYLVQLRTVLMEARLED
                     VVLTFDGLLRRTAFSERMAHMLRVLEHHYHAPVDTEFTVEIKDPHTLNPDVQITLLQC
                     RPQSQLQESRAELPVALREQDIIFSTRRLAPEGMVDKIRTVIFISPEGYFALPSHAER
                     AALSQAISRLNASLVGVNFICVGPGRWGTSTPDLGVNLGYGDIYNARALIELAGEGIG
                     PAPEASFGTHFFQDLVESNIYPLAIYLDDEDAVFNRDFFYDTPNRLTELLPDFEHLTS
                     CLRVIEVASYRPRHHLNLVMDAEKGRSVAFVVPD"
     gene            2462..3115
                     /locus_tag="VN363_08645"
     CDS             2462..3115
                     /locus_tag="VN363_08645"
                     /EC_number="2.7.1.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561207.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0019136 - deoxynucleoside kinase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="deoxynucleoside kinase"
                     /protein_id="HWQ84620.1"
                     /translation="MKKYVAVAGNIGVGKSTLVALLSQRLSWEPFYEPVTENPYLEDF
                     YRDMRTWAFHSQVFFLTRRLRAHRLLMEHPTSVVQDRSVYEDAEIFALNLYRQGLLGE
                     RDYLSYYELYSVLTEFLAPPDLVVYLRATVPTLVQRIQNRSRAYERQIAPDYLSRLNG
                     LYEEWIARFSLCPVLTVPADDLDFVSNPTHMDLVVSKVLDKLMGKDEVVFDGDYRPD"
     gene            complement(3117..>3683)
                     /locus_tag="VN363_08650"
     CDS             complement(3117..>3683)
                     /locus_tag="VN363_08650"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84621.1"
                     /translation="SNAAYSISTGVAEIVPGLGVPLVVTDMVVLTKNQAYLVYKLGLT
                     LGMSTDWQAYVTEFGSVLGSGFIWRQVARSLIGLVPGWGIIPKVVVAYSGTYVVGNVV
                     LQWYLTGRHVTRGQMKELSKQAAEKGKELAGRLTARLPRSQAKVKKTRLLAAKTRQTC
                     QACGKGNTAEAVYCQYCGERLTRPTPGS"
BASE COUNT          748 a         1164 c          952 g          819 t
ORIGIN      
        1 catgtgatca tgcccatgca cctgggaagt tagcccacaa actcgcatgg aatgcaccgc
       61 aggttggcag gtaaatcccg acgccagcct gcggttttta gaaccagact ggaattgacc
      121 caccatgacc ctgctgccgg cttcctctga tcgggctttg agcatctacc tggccctggt
      181 ccaatatcca atcctcagca cccagatccg ttctcgcatg cgccgcgagt tattccggcg
      241 cggggtcatc actccccagg gcttcgaggc tgaagtacgc gagagggctg tccgcaccca
      301 ggccatggaa gggatgcacg atccttttgc cgaagagccc acggatgtct gggaaactcg
      361 tttgatgcac gtccgcgacc acctgaccga tttttacttc gcctacaatt taccttacga
      421 cctcttcgaa aagatcgtcc tggaaaccct ggctgagcgc ggtgcgaaca gcgaggagat
      481 gctggtttcg ttcaaccccg agcttgcccc ccagacaatg ctcttcgagc aggctctggc
      541 gatcgagaaa ctcgcccccg agcagcgcgc tgcggcagat gctcgcctgc aggagatcaa
      601 agtcgtcctg atccgcaccc tgatcagcga ccagctcgct tatctcaaga tcgctaaaga
      661 ctggtttaat gttgccgatc tgatcgagat ccgcaaccgc aaaatcggtc agggcaaaat
      721 cggtgggaag gctgccggta tgctcctggc agcccgcatt ttgatcgagg tcgggggaga
      781 agatattcgc gagcacctgc gcatcccgga gtcgtacttt atcggcgctg acctcaccta
      841 cagcttcatg agcatgaacg gtctgttata ttggggagat cagaaataca aaacggaagc
      901 tcaaatccgg gctgattacc cgcagctgca gcaggaattt atcgccggac agctgcccca
      961 ggatgttgtg gagcgtctgg gcgagctgct ggaagaagtc aaatcgaaac ccctgatcgt
     1021 ccgctcctcc agcctgttag aagataattt cggcacctcc tttgctggaa aatacgaaag
     1081 ccatttctgc cccaatcaag gcacgcccac ccaaaacctg gaagatttga cccgagcgat
     1141 ttccagtatt tacgccagca tcctcaatcc cgatgccttg ctctaccgcc gggcgcgtgg
     1201 actgcaagat tatgatgagc ggatggctat cctgatccag gtcgtagagg gtcagcagtc
     1261 cgggcgctat tttctccccc atgccgccgg ggtagccttc agccgcaatc tttaccgctg
     1321 gtcgccgcag atccgccgcg aggatggctt catgcgcctg gtctggggtc tgggcacccg
     1381 cgccgtagac cgggttggca atgattaccc ccgcctggtt gctctcagcc accctctcct
     1441 gcaccccgag gcttccacaa aggccatccg gcgctattcg cagcgctatg tggatgtaat
     1501 tgatatggaa gccaatgctt tcaagactct gcccgttgga gaagttcttg gctcccacta
     1561 tccacccctc cgctatctgg tacaggtcga ccagggcggc tacctggtcc agctgcgtac
     1621 ggtgctcatg gaagcccgcc tggaggatgt cgtcctcaca tttgatggtc tcctgcgccg
     1681 cactgccttt tcagaacgca tggcgcacat gctgcgcgtc ctcgagcacc attatcatgc
     1741 cccggtagac accgagttca cagtcgagat caaagatccc cacacgctaa atccggatgt
     1801 ccagatcacc ttactccagt gccgcccgca gagccagctt caggagagcc gggcagagct
     1861 gccggtcgct ttgcgcgaac aggatatcat cttctccacc cgccgcctgg ctcccgaagg
     1921 catggtggat aaaattcgca ccgtaatctt tatctccccg gaaggctatt ttgccctgcc
     1981 ctcccatgcc gagcgcgctg ctttgagcca ggccatcagc cgcttaaacg cctcccttgt
     2041 cggcgtcaac tttatctgcg ttggcccagg gcgttgggga acatccaccc ccgacctggg
     2101 cgtgaacctg ggctatggcg atatctacaa cgctcgcgcc ctgattgagc tggccggaga
     2161 agggattggt cctgcgcctg aggcgtcatt tggcacccat ttcttccagg atctggtgga
     2221 atccaatatt tacccgctgg caatctacct ggatgatgaa gacgctgttt tcaaccgcga
     2281 tttcttttac gatacgccca accggctcac tgagctgctc cccgatttcg agcacctcac
     2341 cagctgcctg cgggtgatcg aagtcgcctc atatcgcccg cgccaccacc tcaacctggt
     2401 catggatgcc gaaaagggtc gctcggtggc tttcgtggtg cctgactgaa cggaggttcg
     2461 gatgaaaaaa tatgtcgcgg tagctggaaa tatcggcgtg ggtaaatcta ccctggtagc
     2521 tttactcagc cagcgcctca gctgggaacc tttttatgag ccggttactg aaaaccctta
     2581 cctggaagat ttttaccggg acatgcgcac ctgggcattt cactctcagg tattcttcct
     2641 cacccgccgc ctgcgcgccc accgcctgtt gatggagcat cccacatccg tcgttcagga
     2701 tcgcagcgtt tatgaagatg ctgagatttt cgccctgaac ctctaccgcc agggtctcct
     2761 tggggagcgc gattacctgt cttactacga gctgtattcc gtcctgaccg aatttcttgc
     2821 cccacctgac ctggtggttt acctgcgcgc cactgtaccc accctggtcc agcgcatcca
     2881 aaaccgctcc agggcatacg agcgccagat cgcccccgat tacctgtccc gcctcaatgg
     2941 gttgtatgag gaatggatcg cccgcttcag cctgtgcccg gtgctgaccg tccctgctga
     3001 cgacctggac tttgtctcaa acccaaccca tatggacctg gtggtcagca aagttctcga
     3061 taagctgatg ggtaaagatg aggtggtctt cgacggggat tacaggccgg actgagctaa
     3121 ctcccgggtg taggccgggt cagtcgttct ccacagtact ggcagtaaac cgcctccgca
     3181 gtatttcctt tcccgcacgc ctggcacgtc tggcgcgtct ttgctgccaa caggcgagtt
     3241 ttcttgacct ttgcctgtga gcgcggtaag cgggctgtca gtcgtccagc cagctcttta
     3301 cccttctcgg ctgcctgttt agacagctcc ttcatctgcc cgcgggtgac atgccggccg
     3361 gtcaggtacc actgcagcac cacgtttccc acgacatacg tgccagaata agccacgaca
     3421 actttgggga ttatccccca tcccggcacc agaccaatca ggctgcgcgc cacttgccgc
     3481 cagatgaacc cgcttcccaa cacactcccg aactcagtaa catatgcctg ccaatcggta
     3541 gacattccca gggttaagcc cagcttatac accaggtaag cctgattttt ggtcagcaca
     3601 accatatccg tcacaaccag cggcaccccc agcccaggta cgatttccgc cacccctgtg
     3661 ctgattgaat atgccgcatt gga
//
LOCUS       DATMXK010000472         4569 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_130337, whole genome shotgun sequence.
ACCESSION   DATMXK010000472 DATMXK010000000
VERSION     DATMXK010000472.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4569)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4569)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4569
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130337"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..791
                     /locus_tag="VN363_08655"
     CDS             <1..791
                     /locus_tag="VN363_08655"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWQ84622.1"
                     /translation="LELGWHYLLDWTWILSQLGPVKGKRILDAGAGTGLLQWYLARQG
                     AEVLSVDRLSRANLPVRFRARFDVQGLRPEDLIPLKQQLQQNWASKEKLPARLSRQGR
                     SFLSLAQLGTRSGKVRIYNQDLKTLSDIATDSVDGVVAVSALEHNEPEGLIEVVKELM
                     RVLKPGAPLLATLVASAEQDWWHAPSSAWCYSAGSLRRIFELPSETPDNYADHALLFE
                     KLVNNAELRNGLAKFYFKSGENGMPWGKWDPRYQPVGVCKVKIA"
     gene            840..1097
                     /locus_tag="VN363_08660"
     CDS             840..1097
                     /locus_tag="VN363_08660"
                     /inference="COORDINATES: protein motif:HMM:NF012759.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000036 - acyl carrier activity [Evidence
                     IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl carrier protein"
                     /protein_id="HWQ84623.1"
                     /translation="MEISNPSLAENVRSLLADAFQVSLEEITSDLAFGDLPQWDSMGH
                     MDLMLRLEEAYGIEINAESIAGLVSLPAILEKLENKDQTHE"
     gene            1090..2280
                     /locus_tag="VN363_08665"
     CDS             1090..2280
                     /locus_tag="VN363_08665"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84624.1"
                     /translation="MNESLLVKPRLTIPDHLVGEIQSKLAYVDELISRAVVAPTGDQI
                     EIQLLRSITQAEAQQIEEKIQRVVETMAKGSFKPKVQVLEDHLDKPVPYHLDPMGELL
                     QRGEISQEALGIYALGPLVTRLIEYFERHFLDLADSFEAVRYRFPTLIPAEYMERVSY
                     FQSFPHSLTFATHLRQDIDAIDHFAQHAACEHGVLNVPADSFAGVQALLSPAVCYHLY
                     FALADQPLPNGKIAATAVGNCFRYEAINLTSLERLWNFTMREIIFVGSKDYVLENREI
                     ARQRMQRVMAETGLAYRIESANDPFFIGEFRKQVAFQSAFQLKFEIRASLPFKASTLA
                     VGSYNYHQDFFGRHLNITLPDGSPAHTGCVAFGLERIAFAFLAQYGLEPSGWPAPVRE
                     TVYG"
     gene            2273..2785
                     /locus_tag="VN363_08670"
     CDS             2273..2785
                     /locus_tag="VN363_08670"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84625.1"
                     /translation="MDEVIYRLAEPDDLPVIAGMYAQMDQFFRGFSYRFPEVENIGEA
                     WVDSFRRTLGRFSVVYIAELEGNVVGFMLGRVKRVAPYLGGVMVGELSDMWVEPEARR
                     LGVGKVLSLVTMQWLAAQGVHSIEVQILEGNEPIWRLYEKMGFKPELRQIRMLRADFP
                     PEFDTPQKDA"
     gene            2778..3602
                     /locus_tag="VN363_08675"
     CDS             2778..3602
                     /locus_tag="VN363_08675"
                     /inference="COORDINATES: protein motif:HMM:NF014571.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046353 - aminoglycoside
                     3-N-acetyltransferase activity [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAC(3) family N-acetyltransferase"
                     /protein_id="HWQ84626.1"
                     /translation="MREVSLTQVLEELRRVGVKPGDGLLVHSAIQFLGRPDSGISLYF
                     EALSAVLGLPAQGTLAVPAFNFGFARGQAYDPANTPAEGMGVFSEYVRQQPGAFRTPH
                     PMQSLAIIGVHAPELAQCDTPSAFDPGSAFERMLELDFKLLLLGADIQAASIVHYSEQ
                     RAAVPYRYWKSFQGQIITPPDRAEKMGGQAYRMYVRDLDLDAHLDLHPIQHALQRYGQ
                     WSSTNLNYGTIALCRLQDFVRAADELLAADPWALVGNRATVLQRYQANRSDKQQIQ"
     gene            3605..>4569
                     /locus_tag="VN363_08680"
     CDS             3605..>4569
                     /locus_tag="VN363_08680"
                     /inference="COORDINATES: protein motif:HMM:NF027579.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4910 domain-containing protein"
                     /protein_id="HWQ84627.1"
                     /translation="MLELINELWFLPRELVSDGFDEALFRLAQEIPMQIHRYPSGEQS
                     WTWRIPEKWTCQEAYLETMDGQRLIDYADHPLHVISYSLPFEGVVSSEELLKHLHVHP
                     RLPEAIPFVFKYYERDWGLCCSQALRASLQDTHYRVVIRTTFEPGELKVGEVIIHGQS
                     SQTVMLVAHLCHPAMVNDDLTGVVVGLEVARELLRQVSAGKKPYYTYRLLILPETIGS
                     VAYLSHHEHLIPDMAAGLFLEMLGNDSPHALQHSFQSQSQIDKCLSTTLPGLDPQSYT
                     GAYRSVINNDERQFNAPGVRVPMLSLSRVESPESPTRPYREYHSS"
BASE COUNT          997 a         1278 c         1238 g         1056 t
ORIGIN      
        1 acctggagct gggctggcat tatcttttgg actggacctg gatcctctct cagctcggtc
       61 ctgtaaaagg gaagcgcatc ctggatgcag gtgccgggac tggattactg cagtggtatc
      121 ttgcccgaca gggggcagaa gtcttaagcg tggaccgtct cagccgggca aatttgcctg
      181 tgcgcttccg cgcccggttc gatgtccagg gactgcgacc agaagatctg atccctctca
      241 aacagcagct ccaacaaaac tgggccagca aggagaagct gccagccaga ttgagccgcc
      301 aggggcgttc atttctcagc ctggcccagc tcggcactcg ctctggaaaa gtcaggatat
      361 ataatcagga cttgaaaacc ctgagcgata tcgctaccga ttcagtagat ggtgtggtcg
      421 ctgtatcagc gcttgagcat aacgagccag aaggcctgat cgaagttgtc aaagagctga
      481 tgcgggtgct caaacctggt gcgccattgc tggctactct ggtagcctcg gctgaacagg
      541 actggtggca tgcgccttcc agcgcctggt gttattcagc cggcagcctg cgccgcatct
      601 tcgagctgcc atctgaaacc ccggataact atgcagacca cgccctgtta ttcgagaaat
      661 tggtgaataa cgccgaatta aggaatggtc tggcgaaatt ctattttaaa tcaggcgaga
      721 atggaatgcc ctggggcaag tgggacccgc gctaccagcc ggttggtgtc tgcaaggtaa
      781 aaattgcctg acaagaacca gctcaaatat caatttacac cagaaagaac ccctgaatta
      841 tggaaatttc aaatccttcc ttagctgaaa acgtacggtc gctgctggcg gatgcatttc
      901 aagtttcgct tgaagagatc acatcggatc tggcttttgg cgatctgcca caatgggact
      961 caatgggtca tatggatctg atgctgcgcc tggaagaagc ctacgggatc gagattaacg
     1021 ccgaatcgat tgccggattg gtcagcctgc cggcaatttt ggaaaagctt gaaaacaagg
     1081 atcaaactca tgaatgaatc cctcctggtc aagccccggt tgacaattcc cgatcatctg
     1141 gtcggtgaaa ttcaatcaaa actggcctat gttgatgagc tgatctctcg cgcagtcgtt
     1201 gccccgacag gcgaccagat tgaaatccaa ctcttgcgct cgattaccca ggccgaggcg
     1261 caacagatag aggaaaaaat ccagcgggtc gtcgaaacca tggccaaagg aagttttaaa
     1321 cccaaggtcc aggtcctgga agatcacctg gacaaacccg taccctacca cctggatccg
     1381 atgggcgagc tgttgcagcg cggcgagatc agccaggaag ccctggggat ttatgccctg
     1441 gggcctctgg ttacccgcct gatcgaatat ttcgagaggc attttcttga cctggcagac
     1501 tcattcgaag ctgtccgcta ccgtttccct accttgatcc cggctgaata tatggagcgg
     1561 gtcagctact tccaatcttt cccccactcc ttgactttcg cgacccacct gcgccaggat
     1621 attgatgcca ttgaccattt tgcccagcat gctgcctgtg agcacggcgt gctcaacgta
     1681 ccagctgatt cctttgccgg agtgcaggcc ttgttgtcac ccgccgtgtg ctatcacctc
     1741 tactttgccc tcgccgacca gccgctgccg aatgggaaga tagcagctac agcggtaggg
     1801 aactgtttcc gatatgaggc gatcaacctg acctcacttg aacgcctgtg gaatttcacc
     1861 atgcgcgaaa tcatcttcgt cggctccaag gattatgtcc tcgagaaccg ggaaatcgct
     1921 cgccagcgaa tgcagcgggt gatggcggag accggtctgg cttaccgtat tgagagcgcc
     1981 aatgacccat tttttatcgg tgaatttcgt aaacaggttg catttcaaag cgccttccag
     2041 ctcaagttcg agatccgggc cagcctgcct ttcaaagcca gtactctggc agtaggctcc
     2101 tacaattatc accaggactt cttcggacgc cacctgaata tcaccctgcc ggatggctca
     2161 cctgcccata ccggatgtgt ggctttcggt ctggaacgga ttgcctttgc attcctggcc
     2221 cagtacggac tcgagccgtc tggctggcca gcgccagttc gcgagacagt ctatggatga
     2281 ggtaatctat cgcctcgcag aaccggatga tctgccggtc attgccggga tgtacgccca
     2341 gatggaccag tttttccgcg gtttcagcta tcgttttccc gaagtcgaga atatcggcga
     2401 ggcgtgggtc gattcgttcc gccgcaccct ggggcggttc agtgttgttt acatagccga
     2461 gctggaagga aatgtggtcg ggtttatgct ggggcgggta aaacgagtgg ccccatatct
     2521 ggggggcgtg atggtgggag agctgagcga tatgtgggta gaaccagaag cgcgccgcct
     2581 gggtgtcgga aaagtgctgt ccctggtcac catgcagtgg ctggcagccc agggcgtcca
     2641 ttccatcgag gtccagatcc tggaaggcaa tgagccgatc tggcggttgt acgaaaagat
     2701 ggggttcaaa cccgagctgc gccagatccg catgcttaga gcagattttc cacctgagtt
     2761 cgatactcct caaaaggatg cgtgaagtta gcctgactca agtccttgag gaactgcggc
     2821 gcgtcggcgt gaagcctggt gacggcttgc tggtccactc ggcgatccaa ttcttaggca
     2881 gaccagacag cggaatcagc ttatattttg aagctttgag cgccgtgctc ggcctccctg
     2941 cgcaaggcac gctggctgtg ccggcattca atttcggttt tgcccgcggc caggcttatg
     3001 accccgccaa cactcctgct gaaggcatgg gtgtattctc tgaatatgtc cgccagcagc
     3061 caggcgcatt ccgcaccccg catcccatgc agtctctggc aatcattggc gttcacgcgc
     3121 cggagctggc gcagtgcgac accccctctg cattcgatcc cggttctgct tttgagcgca
     3181 tgctcgagct tgatttcaaa ctgctgcttt tgggcgcgga tatccaggcg gcatccattg
     3241 tccactacag cgagcagcgc gccgccgttc cctaccggta ttggaaatcg tttcagggtc
     3301 agatcatcac ccctcctgat cgggctgaga aaatgggagg ccaggcttac cggatgtatg
     3361 tgcgcgatct cgatttagac gctcatctgg atctccatcc catccaacac gctttgcagc
     3421 gctatgggca gtggtccagc acaaatttga attatggcac gattgctttg tgccgtttgc
     3481 aagatttcgt tcgtgctgcc gatgagctgc tggcagccga tccctgggcg ctggttggaa
     3541 atagagccac cgttctgcag cgctatcagg ctaaccgatc tgataaacaa caaattcaat
     3601 gagtatgctg gaattaatta acgagctctg gtttttaccc cgcgagctgg tctcggatgg
     3661 ctttgatgag gccctgttcc gcctggcaca ggaaatcccg atgcaaatcc accgctaccc
     3721 gagcggcgaa caatcctgga cctggcgtat ccctgagaaa tggacctgcc aggaagctta
     3781 cctcgaaacc atggacgggc agcgtctgat cgactatgcc gaccatcccc tgcatgtgat
     3841 ctcgtactcc ctgccgtttg agggagtggt gtccagcgag gagcttctca agcatctcca
     3901 tgttcacccc cgcctcccgg aggcgatccc atttgtattc aaatattatg aacgcgattg
     3961 ggggctgtgc tgctcgcaag cgctgcgcgc cagcctgcag gatacccatt acagggttgt
     4021 gatccggaca acatttgaac ccggtgagct taaggtcggg gaagtcatta tccatgggca
     4081 gtcatcccag actgtcatgc tcgtcgctca cctatgccac ccggcgatgg ttaacgatga
     4141 tctgaccggc gtcgtggtag ggctggaggt ggcgcgggaa ttactccgcc aggtaagcgc
     4201 tggaaagaag ccttattaca cctaccgttt attaatcctg cccgaaacga tcggctcagt
     4261 agcttacctc agccaccatg aacatctgat ccccgatatg gctgccggct tatttctgga
     4321 aatgctgggc aatgattctc cgcatgcctt gcagcattct tttcaaagcc agagccagat
     4381 tgataaatgc ctcagcacaa cccttccagg ccttgacccg cagtcttaca caggtgccta
     4441 ccgctccgtc attaacaatg acgaacgcca gttcaacgct cccggtgtgc gcgtgccgat
     4501 gctctcgtta tcccgggtag aatcacctga atcacctacc cggccctacc gtgaatatca
     4561 ttccagcga
//
LOCUS       DATMXK010000473         4865 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_10495, whole genome shotgun sequence.
ACCESSION   DATMXK010000473 DATMXK010000000
VERSION     DATMXK010000473.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4865)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4865)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4865
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_10495"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..984)
                     /locus_tag="VN363_08685"
     CDS             complement(<1..984)
                     /locus_tag="VN363_08685"
                     /EC_number="2.7.7.12"
                     /inference="COORDINATES: protein motif:HMM:NF008724.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008108 - UDP-glucose:hexose-1-phosphate
                     uridylyltransferase activity [Evidence IEA];
                     GO_process: GO:0006012 - galactose metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="UDP-glucose--hexose-1-phosphate
                     uridylyltransferase"
                     /protein_id="HWQ84628.1"
                     /translation="MQNVPHRRLNSLTGEWVLVSPHRARRPWLGQIEKLPPDDRPAYD
                     PGCYLCPGNERAGGVRNPVYASTFVFTNDFSALLPGEPDEPLEDHPLLSTQAVQGTCR
                     VICFSPRHDLTLPEMALADIQRVVAVWIEQVQELSQSYRWVQVFENKGAIMGSSNPHP
                     HGQIWASSTLPNEPAKEDHHQSVYIARHGRALLMDYLAVELERGERLVVENEDWVALT
                     PYWAIWPFEILLLPRRPIKRLPELSAGEQESLARILKNLLSRFDNLFETSFPYSMGWH
                     GAPGPGSLHPGAPGPESAGIVDVTLDSASEDAGWQLHAHFYPPLLRSATVKK"
     gene            1105..2238
                     /locus_tag="VN363_08690"
     CDS             1105..2238
                     /locus_tag="VN363_08690"
                     /inference="COORDINATES: protein motif:HMM:NF012488.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alanine--glyoxylate aminotransferase family
                     protein"
                     /protein_id="HWQ84629.1"
                     /translation="MNAPNPAESFPELNLPVRTLLGPGPSTADPRVLRAMAAPLVGHL
                     DPTFLALMDRTQVLLRQVFETENAFTLAVPGTGSAGMEAALANVIEPGDAVLVCSNGF
                     FSLRMAEMARRFGGEVRLITRPWGEVFTAQEIQAELAARPAKIVALVHAETSTGALQP
                     LNEIAAVVHAHGALFLVDAVTSLGGLPVRVDEVGIDICYSGSQKCLGCPPGLAPITLS
                     PAAEEVLQRRKAPLPGWYLDLTLLQKYWGSPRLYHHTAPISTHYALYEGLRLAVSEGL
                     AARWERHARNARLLWDGIESLGLSLHVPLEYRLPSLTTVRIPEGVDDLQIRSRLLNEY
                     NIEIGGGLGELKGKVWRVGLMGYASRPENVLLLLAALKTLLGY"
     gene            2413..2982
                     /locus_tag="VN363_08695"
     CDS             2413..2982
                     /locus_tag="VN363_08695"
                     /inference="COORDINATES: protein motif:HMM:TIGR02937.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ84630.1"
                     /translation="MLQSFSRWFQGVKQDVPLVNPEAFSQLYELNYRSVFRYLFGLHG
                     GPLEDIEDLTAETFIHAWRARHTFSGDTPNNATAWLLRIARRLVIDNYRREQARIQTE
                     EELADDLPQLGPTPENLVQIGEQYETLWRLLQRLPGRQREMLVLRYFLDWRVTQIGEY
                     MALPENTVSVTIRRGLARLQQEWPADKEI"
     gene            2984..4837
                     /locus_tag="VN363_08700"
     CDS             2984..4837
                     /locus_tag="VN363_08700"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84631.1"
                     /translation="MSRTTDDKSQADRIEELLESIRPEPGESYRKRMAQAPWVRIPAH
                     KQAARARTFRFTAITASILLLGTILLATPVGNVVANGILQLFTRAQSNTLPVPDDQYV
                     PPIPSATPASPHDLALLPAEQVATTTPTPEPTAAEYPGMPRELSLIEAEAIAGFDLYE
                     PLRLPRDYRLTRITYNTYHQAIEMEYTSPRAATGESFYIIQGKNLASATVGADAKIET
                     FQVGGHTAELVRGIWFADAGSTQSTWQEGPEWYTIRWQTQDITIEIGFRLNETFYPAY
                     INHDEMIVLARDVVRCPATADFNCQLSQAAAAAGFTPWLLSTPPSGFSFQGSDYRPGL
                     TILSYSNGAQQLSVLQSTTQASLLQSDAIWSSAPVSAIQAVDVAGSPGEMIQGMPDSP
                     STWDPEAAVERLRWENGDYWFQIIATGGLAPVQTPDLITIASQLTPDESLAQLGRQPT
                     PAPPSDWAEAYTTVAEVESMAGFDVLEPSVLPEGLSFSHARYLPDPGVVMLFYGSFAS
                     DLMHSTGPLLRIHQQMLINSESAIPEDYPPEAIEFVTIHGFPGILRTGSLHTKAAVPG
                     GPTPAPNWSDDGTLSLSWETDKMQIYINFSPTSNGKRITREDLLRIAESLQ"
BASE COUNT         1048 a         1498 c         1327 g          992 t
ORIGIN      
        1 ctttttcacc gtcgccgagc gcagcagcgg ggggtaaaaa tgggcgtgca actgccagcc
       61 tgcatcctcc gaggcgctgt ccagggtgac atccacaatc cccgcggatt caggtccagg
      121 agcgccgggg tgaagagaac ctggacctgg tgcgccatgc cagcccatgg aatagggaaa
      181 ggatgtctca aacagattgt caaaacggga taataaattt ttcaggatgc gcgccaggga
      241 ttcttgctcg ccagcggaca gctcagggag gcgcttgatg ggccggcgcg gcagcagcag
      301 aatctcaaac ggccagatcg cccaataggg tgtcagcgcc acccagtcct cattctctac
      361 caccagacgc tcaccgcgtt ccagctccac agccagataa tccatgagca gtgcccgccc
      421 atgccgggct atataaacgc tctggtggtg gtcttctttc gccggctcgt tggggagcgt
      481 cgagctcgcc cagatctgcc cgtgtgggtg cgggttggag ctgcccatga tcgcgccctt
      541 gttctcaaac acctgcaccc agcgatagct ctggctcaac tcctgcactt gctcgatcca
      601 caccgccacc acccgctgga tatctgccag cgccatctcc ggcagggtca ggtcgtggcg
      661 cggcgagaag cagattaccc ggcaggtgcc ctgcacagcc tgggtgctca ataagggatg
      721 gtcttccaga ggttcatccg gctcacccgg cagcagcgcg gagaaatcat tcgtaaaaac
      781 aaaggtgctg gcatagaccg ggttacggac cccaccggcg cgttcattac cggggcataa
      841 atagcagccc gggtcatagg ccgggcgatc gtccggcggc agtttctcta tctgcccgag
      901 ccaggggcgc cgcgcccggt gcggtgagac cagcacccat tctccggtca gggaattcaa
      961 acggcgatgt gggacgtttt gcacggcgct acctccagta gataccctgg gttttgatta
     1021 atcgattcta ccccgaaaac aaaatccgac tggattacaa tacaggcaaa cccctcccga
     1081 aacatttacc caccgaggtg aaaaatgaac gctccaaatc cggccgaatc cttccccgag
     1141 ctaaacctgc ctgtccgcac cctgctcggt cctggtccca gcactgccga tccacgcgtg
     1201 ctgcgcgcca tggctgcccc cctggttggg catctcgacc cgacctttct ggccctgatg
     1261 gaccgcaccc aggtcttact gcgccaggta tttgaaaccg aaaatgcctt cacgctggca
     1321 gtcccgggaa ccggctcggc cggtatggaa gctgccctgg caaacgtgat cgagcccggc
     1381 gatgcggtcc tggtgtgcag caacggcttc ttcagcctgc gcatggctga gatggcgcgc
     1441 cgcttcggcg gtgaggtgcg cctgatcacc cgcccctggg gagaggtatt cactgcccag
     1501 gaaatccagg ccgagctcgc cgcccggccg gcgaaaatcg tagccctggt ccacgccgag
     1561 acttccactg gcgcactcca gcccctgaat gagatcgccg cggtggtgca tgcccacggt
     1621 gctttatttc tggtcgatgc ggtcacctcc ctgggagggc tgccggtgcg cgtggatgaa
     1681 gttggcattg atatctgcta cagcggctcg cagaaatgcc tcggctgccc gcctgggctg
     1741 gctccgatca ccctcagccc cgccgccgaa gaagtcctgc agcggcgtaa agcccccctg
     1801 cccggctggt acctggacct gaccctgctg caaaagtact ggggcagccc acgcctgtac
     1861 caccacaccg cccccatcag cacgcactat gctctctacg aaggcctgcg cctggcagtg
     1921 agcgaaggcc tggcagcgcg ctgggaacgc cacgcccgca acgcccgcct gctctgggat
     1981 ggtatcgagt cgcttggact gagcctgcat gttcccctgg aataccgcct gcccagcctt
     2041 accaccgtgc gcatcccgga aggcgtggat gatctgcaaa ttcgcagccg cctgctcaac
     2101 gaatacaata tcgagattgg cggcgggttg ggcgagctga aaggcaaggt ctggcgcgtc
     2161 gggttgatgg gctacgcctc gcgccccgag aatgtgctct tgctcctggc ggcattgaaa
     2221 acgctattag gatattaaat ctatccggct tcgaattgaa taacctgaat tgttttttaa
     2281 ttagcgtctt gtggcgacct ttagacgtct ctttatacat ccctgtcaga tattccccca
     2341 ttttgcgttt acatatatga aacctggcca tgtttcaata acccggagaa tatctcaatg
     2401 aaggatgcca gcatgctcca gagtttttcg cgatggtttc aaggtgtaaa gcaggatgtg
     2461 cccctcgtca atccggaagc gttcagtcag ctttatgagc tgaactaccg gtccgttttc
     2521 cgctaccttt tcgggctgca cggggggccg ctggaagata tcgaagacct gaccgctgag
     2581 accttcattc acgcctggag agctcgccac accttttctg gtgacacgcc taacaatgct
     2641 acagcctggt tactgcggat tgctcgccgg ctggtcatcg acaattaccg acgcgagcag
     2701 gcgcggatcc agacggaaga agagcttgcg gacgatctcc cacagcttgg accaaccccg
     2761 gagaatctgg ttcagatagg cgaacaatac gaaacgctgt ggaggcttct gcagcgcctt
     2821 cccggcagac agcgagagat gctggtgttg agatattttc tggactggcg cgttacccag
     2881 atcggcgaat acatggcgtt acctgagaat accgtctcag tcacgatccg ccgcgggctg
     2941 gcgcgattac agcaagaatg gcctgctgac aaggagatat gaaatgtcca gaacaactga
     3001 cgataaatcc caggcagatc ggatcgagga gcttctcgag tcgatccgac ccgagccagg
     3061 cgaatcctac cggaaacgca tggctcaagc cccctgggta cgaataccag ctcacaagca
     3121 agcggcccgg gcaagaacat ttcgttttac ggcaattaca gccagcatcc tgctgctagg
     3181 tacgattctg ctcgcaactc cagttgggaa tgtagtcgca aatgggattc tgcagctctt
     3241 cacccgggcg cagagcaata ccctgccagt accagatgac caatacgttc cgcccatccc
     3301 ttcagccacc ccggcgtccc ctcacgacct ggcgctgctg ccagctgagc aggttgccac
     3361 gaccacaccg actcctgaac caacggcagc tgagtaccct gggatgccca gggagctgag
     3421 cctgatcgag gccgaagcga ttgccggctt tgatctgtac gaaccgttgc ggctgccgcg
     3481 cgattatcgc ctgacgcgca tcacctacaa tacataccac caggcgatcg agatggagta
     3541 cacctcgcct cgggctgcga cgggcgaatc attctacatt atccagggaa agaacctggc
     3601 atccgcaacc gtaggcgcgg atgcaaaaat cgagactttt caggtaggag gccacaccgc
     3661 cgagctcgtg cgcggcatct ggttcgcaga tgcagggtcc acccagtcca cctggcaaga
     3721 agggcctgaa tggtacacca ttcgctggca aactcaggat atcaccattg agatcgggtt
     3781 cagattgaac gaaactttct acccggcata tatcaaccac gacgaaatga tcgttctcgc
     3841 ccgagacgtt gtacgctgcc cggcaacggc agatttcaac tgccagctaa gtcaggccgc
     3901 agcagcggct ggcttcacgc cctggctgct ttctacccca ccatctggtt tttcttttca
     3961 agggtcggat taccgccccg ggttaactat cctgtcatac agcaacggcg cacagcagct
     4021 tagcgttctc caatccacca cccaggcctc ccttctccaa tcagatgcga tctggtccag
     4081 cgcgcctgtt tcagctatcc aagccgtgga tgtggctggt tcacctggcg aaatgataca
     4141 aggcatgccg gattctccat ccacctggga tcctgaggca gccgtagagc gcttgcgttg
     4201 ggagaacggc gattattggt tccagattat cgctaccgga gggcttgctc cggtacagac
     4261 cccagatttg atcacgattg ccagccagct gacaccggat gagtctctgg ctcagctggg
     4321 tcgccaaccg acacccgctc ccccgtccga ctgggccgag gcctatacca ctgttgccga
     4381 agtagaatct atggctggct tcgatgtcct tgagccctct gtgctgccag aaggattatc
     4441 tttcagccat gctcgttatt tacccgaccc cggtgtcgtg atgcttttct atgggagctt
     4501 tgcatcagac ttaatgcaca gcacaggccc gcttttacgc atccaccaac agatgctaat
     4561 caacagtgaa agcgcaattc ccgaggacta cccgcctgaa gcaatcgagt tcgttaccat
     4621 ccacggattt cctggtattc tgcgtacggg aagtttgcac acgaaagcgg cggtccctgg
     4681 cggaccaaca ccggctccaa actggtctga cgatggcacc ctgagcttga gttgggagac
     4741 ggataaaatg cagatttata tcaatttttc acccaccagt aatggcaaac ggattaccag
     4801 agaagatcta ttaaggatag ccgaaagcct gcaataacca tttcgcaagt aggtacccca
     4861 agcgc
//
LOCUS       DATMXK010000474         2977 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_5904, whole genome shotgun sequence.
ACCESSION   DATMXK010000474 DATMXK010000000
VERSION     DATMXK010000474.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2977)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2977)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2977
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5904"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /gene="pstC"
                     /locus_tag="VN363_08705"
     CDS             <1..741
                     /gene="pstC"
                     /locus_tag="VN363_08705"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561147.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease subunit PstC"
                     /protein_id="HWQ84632.1"
                     /translation="FFGGTKWQPQIAEFGVWPLVTSTLITTTIAMLIAVPLGLMVAIY
                     LSEYASSKARNLLKPILEVLAGIPTVVYGYFALTFMTPVLRGIFGRDVVGVYNTASAG
                     IVMGILILPLITSMSEDALSAVPRALREAAYGLGATKLETAVKVVVPAALSGIAAAVI
                     LGISRGIGETMIVALAAGAGPNFTFNPFEGAETLTGHIVRISGGDLSYNSIDYNSIFA
                     LGLLLFVMTLSLNIISGIIVRRFRETYE"
     gene            738..2003
                     /gene="pstA"
                     /locus_tag="VN363_08710"
     CDS             738..2003
                     /gene="pstA"
                     /locus_tag="VN363_08710"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561148.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter permease PstA"
                     /protein_id="HWQ84633.1"
                     /translation="MNPSQLSIPPGTAGPEFQPNLPGRHRSSRIWRKVFQISTIIGLI
                     ALTLLLFNVSNSAFGYVAYKFRVDPATLALDGVVLEDMDHTQLTMVLSQNISANAYKG
                     LDRDKPIATRSESELRTLIAQRITRPEVLGVWSLSDSIFNRSKVEAEAIAIDPDATLV
                     FKSWLTADFIQAPQAADPLWAGVWYAINGSLWTILICIVVAFPLGISAAIYLEEYSTD
                     SWMQRIIQTNINNLAGVPSIIYGILGLALFVRALEPLTSGAIFSLTDATTANGRTILS
                     AGLTLALLILPVIIINSQEAIRAVPGSLREASYGLGATRWQTVWSHVLPNAIPGILTG
                     TILSVSRAIGETAPLVVVGASTYLTFNPDGPFSKFTTLPIQIYQWTSRPQAEFRSAAG
                     AAILVLLVLLLSLNAAAILLRNRYSRRLQ"
     gene            2000..2815
                     /gene="pstB"
                     /locus_tag="VN363_08715"
     CDS             2000..2815
                     /gene="pstB"
                     /locus_tag="VN363_08715"
                     /EC_number="7.3.2.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561149.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015415 - ATPase-coupled phosphate ion
                     transmembrane transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="phosphate ABC transporter ATP-binding protein
                     PstB"
                     /protein_id="HWQ84634.1"
                     /translation="MNLTTNQTTTPPTLASDRRFAIEARNLSLYYGEFRAVKNVNLQI
                     EKQKITAIIGPSGCGKSTMLRSFNRMNELVPSAHAEGEVLFHGQNLNDPHVDPVEVRR
                     RIGMVFQKPNPFPKSIYENIAWGARINGFRGNMDDLVEESLRGAALWDEVHDKLDQSG
                     LSLSGGQQQRLCIARTIAIKPDIILMDEPASALDPIATLRIEELMRELVQNYTIIVVT
                     HNMQQAARVSDFTAFFTMDEDRAGYLKEFGITDQIFTNPKDQVTEDYITGRFG"
BASE COUNT          619 a          913 c          778 g          667 t
ORIGIN      
        1 ttttttgggg gaacgaaatg gcagccgcag attgctgagt ttggcgtctg gccgctggtc
       61 accagcacgc tcataaccac aacaattgcc atgctgatcg ccgttccgct gggactgatg
      121 gtggcgattt atctgagcga atatgccagc tcaaaggccc gcaatctcct caaaccaatc
      181 ctggaagtgc tggcaggtat ccccacggtc gtctatggat attttgcact gaccttcatg
      241 accccggtct tacgcggcat ttttgggcgg gatgttgtcg gagtctacaa tacggcttcg
      301 gccgggattg taatgggcat tctgatcctt ccgctaatca cctccatgag cgaggatgcg
      361 ctcagcgcag tgccccgggc cttgcgcgaa gcagcctatg ggttgggcgc caccaagctg
      421 gaaactgctg tcaaagtggt agtcccggca gccctgtcag gcatcgctgc agcagtcatt
      481 ttaggcatct cgcgggggat tggtgaaacc atgatcgtcg ccctggctgc cggcgccggg
      541 ccaaatttca ccttcaatcc cttcgaaggc gctgaaacac tcaccggtca catcgtgcgc
      601 atcagcggcg gtgatctgag ctacaactcc atcgactaca acagtatctt tgcgctcggt
      661 ctcttgctgt ttgtgatgac gctctccctc aatatcatca gtggcatcat cgttcgccgc
      721 ttccgggaga cttacgaatg aatccgtccc agctctccat cccccccggc accgccggtc
      781 cggagttcca accaaatctc cctgggcgcc accgcagcag ccgtatctgg cggaaggtct
      841 tccagatctc cacgatcatc ggtttgatcg ccctgactct cctgcttttc aatgtctcca
      901 acagcgcttt tggttatgtc gcttacaagt ttcgcgtcga tcctgccacg ctggctctgg
      961 acggagttgt cctggaagat atggaccata ctcagctcac aatggtgctc agccagaata
     1021 tctcagccaa cgcctacaaa ggcctggatc gcgataaacc catagccacg cgcagcgaaa
     1081 gcgagctgcg aacattgatc gctcaacgta tcacgcgccc cgaagtctta ggggtctgga
     1141 gtctgtccga ttccattttt aatcggtcga aagtcgaagc cgaagccata gccatcgatc
     1201 cggacgccac gctggtattc aagagctggc ttactgccga ttttatccag gccccccagg
     1261 ccgctgatcc gctgtgggcg ggtgtctggt atgccatcaa cggctcgctg tggaccatcc
     1321 tgatctgcat cgtggttgct ttcccgctgg ggatttccgc agccatctac ctggaagaat
     1381 actccaccga cagttggatg cagcgcatca tccagaccaa tatcaacaac ctggccggag
     1441 tgccttccat catttacggc atccttggat tggcgctctt cgtgcgggcg cttgaacccc
     1501 tcaccagtgg ggcgatcttc agcctcaccg atgccaccac cgccaatgga cgtacgatcc
     1561 tatcggcagg cctcaccctg gcgctgctga tcctgccggt gatcattatc aactcccagg
     1621 aagccatccg cgccgtaccc ggctcgctgc gcgaggccag ctatgggctg ggtgcaactc
     1681 gctggcaaac cgtctggtcg cacgtgctgc ccaatgccat ccccggcatt ctcaccggca
     1741 cgatcttatc cgtatcacgg gcaattggtg aaaccgctcc cctggttgta gtgggcgcgt
     1801 cgacttacct gaccttcaat cccgacggcc cgttctccaa attcaccacc ctgcctattc
     1861 agatttacca gtggacctcg cgcccccagg cagaatttcg ttctgccgcc ggtgctgcga
     1921 tcctggtcct gctggtgctg ctgctcagct tgaatgcagc cgcgatctta ctccgcaatc
     1981 gttacagccg gaggctgcaa tgaacttgac gaccaatcag actaccaccc ctcctacact
     2041 cgccagtgac aggcgcttcg ccatcgaagc ccgcaatctg agcctgtatt atggcgagtt
     2101 tcgggcggtg aaaaatgtaa atttacagat cgaaaaacaa aaaattaccg ccattatcgg
     2161 gccctcgggc tgcggcaaga gcaccatgct gcgctcgttc aaccggatga acgagctggt
     2221 acccagcgcc catgcggagg gtgaagttct tttccacggg cagaacctga atgatccgca
     2281 tgtcgacccc gtcgaggtcc gccgccggat cgggatggtc ttccagaaac ccaatccctt
     2341 cccgaaaagc atctatgaga atatcgcctg gggtgcccgc atcaacggct tccgcggcaa
     2401 catggatgat ctggtggaag aatccttgcg tggggcggcc ctgtgggatg aggtgcatga
     2461 caagctggat caatccgggc tgtccctttc cggtgggcag cagcagcgcc tgtgtatcgc
     2521 ccgcaccatt gccatcaaac ctgatatcat cctgatggac gagccagcct ccgccctcga
     2581 cccgatcgct accctgcgga tcgaagagct gatgcgggag ctggtgcaga actacaccat
     2641 catcgtcgtt acccacaata tgcagcaggc ggcgcgggta tctgatttca ctgccttttt
     2701 caccatggat gaagaccgcg ccggttacct caaagaattc ggcatcaccg atcagatctt
     2761 caccaatccc aaggatcagg ttaccgaaga ttacatcacc gggcgtttcg gataagcgcg
     2821 tgtggattaa tcagtaaagg aattatttgt tatgtcccga gaaaccttag accgccgcat
     2881 caggcacctg ctggatgaga tcctgatcat ggacagcatg gtggagcaag ccacggttga
     2941 tgcagttgcc gctcttaaag cccgcgatgt tgagaat
//
LOCUS       DATMXK010000475         2340 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_84049, whole genome shotgun sequence.
ACCESSION   DATMXK010000475 DATMXK010000000
VERSION     DATMXK010000475.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2340)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2340)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2340
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84049"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..621
                     /locus_tag="VN363_08720"
     CDS             <1..621
                     /locus_tag="VN363_08720"
                     /inference="COORDINATES: protein motif:HMM:NF019265.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0016987 - sigma factor activity [Evidence
                     IEA];
                     GO_process: GO:0006352 - DNA-templated transcription
                     initiation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sigma-70 family RNA polymerase sigma factor"
                     /protein_id="HWQ84635.1"
                     /translation="MANQVSPPPAVDFSTLSDTDLLDWIERAEETALGTLYDRYQRLV
                     FSLAYQSIGEQGLAEEITQDVFLRIWEKAGTYRPDQGKVLTWIVSITRNRAIDMYRRQ
                     RIRPEKNSLDFEDLHPREQRDGVDIEAEVELSLQQQRIRAALASLPPEQRQALSLAFF
                     RGFTHTEIAARLHEPLGTIKTRIRAAMHKLHHQLTDENSYGRDESY"
     gene            649..1407
                     /locus_tag="VN363_08725"
     CDS             649..1407
                     /locus_tag="VN363_08725"
                     /inference="COORDINATES: protein motif:HMM:NF021604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="anti-sigma factor"
                     /protein_id="HWQ84636.1"
                     /translation="MENIDPIRELIPAYALDSLDPEEAELVARHLAECPECQLEFASY
                     SELVGSLALAVPMHPAPPELKARILSAAHASAQSMPLPANYPQTSRIAKPARAKSGWA
                     NIFQQALPAWGLLSILLIGILAISNLRLNQRIASLEAARPEFATVQLSGADNSPNAVG
                     MLVISPDGQNGSLVVDQMPVLEDNQDYQLWLIKDGQRTSGGVFHSYSNGYGVLWIHAD
                     QPLISYEEVGVTIEPAGGSPAPTGAKIIGGLIQN"
     gene            1431..>2340
                     /locus_tag="VN363_08730"
     CDS             1431..>2340
                     /locus_tag="VN363_08730"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84637.1"
                     /translation="MTLHLPYTYAWQNPVLRETIYPYRLEKLRDFLLVYEEIDLWARL
                     KNSPVDPALEQEIRQELPGLQAELSTGQAQQKTIQAALSLLVKKLSQIERLPDVRKLV
                     LEIARLEQETARLNIRRALLEGYIQWSLKIAPENDPVRPKRQAELEQVQTQLSDLASQ
                     ISSLKQNYAEVFLPLKEQEDKLEASADELAERIADLLDRLRGIPLFSTAQPVTPKIAA
                     RWALNKYRQQLEGLNQEELLALVMERFQRQPERYPGWLQYMVIHFSGMRYKSAHGSWA
                     DPRLLLQKIRADELEEQLNSASEAHLQ"
BASE COUNT          542 a          702 c          642 g          454 t
ORIGIN      
        1 atggcaaatc aagtctctcc cccgccggca gttgattttt ccacgctcag cgatacagat
       61 ttattggatt ggatcgaacg cgccgaagaa actgccctgg gtacgctcta tgaccgctac
      121 cagcgcctgg tcttcagcct ggcttaccag agtatcggcg agcaagggct ggcagaagaa
      181 atcacccagg atgtattcct gcgtatctgg gagaaagccg gcacctatcg ccccgatcag
      241 ggcaaggtgc ttacctggat tgtatcgatc acccgcaacc gggccatcga tatgtaccgg
      301 cgccagcgca tccgcccgga aaagaactcg ctcgattttg aagacctgca cccccgcgag
      361 cagcgcgatg gtgtggatat cgaagctgaa gtcgaactgt ccctccagca gcagcggatc
      421 cgggctgccc tggccagcct accccccgaa caaaggcagg cgctcagcct ggctttcttc
      481 cgcggcttca cccataccga aatcgctgcc agactgcacg aacccctggg aacgatcaag
      541 actcgcatcc gggctgcgat gcataagctc caccaccagc tcacggatga aaacagttat
      601 ggaagggacg aaagctatta atcaaagctc gtatgcagat agtgagttat ggaaaacatt
      661 gatcccatcc gggagctgat ccccgcctac gctctggaca gcctcgatcc tgaagaggct
      721 gagctggtcg cgcgccatct ggcggagtgc cctgagtgcc agcttgaatt cgccagttac
      781 agcgagctgg ttggcagcct ggctctcgcg gttcctatgc acccggctcc tccggagctg
      841 aaggcgcgca tcctgtctgc cgcacatgcc tctgctcaat ccatgccttt gcctgcgaat
      901 tatccccaga ccagccggat tgcgaagcct gcccgggcga aatctggctg ggcgaatatt
      961 ttccagcagg ctttaccagc ctggggactc ctgagcatcc tcctgatcgg catcctggcg
     1021 atcagcaact tgcgcctgaa ccagcggatt gccagcctgg aagcagcccg cccggaattc
     1081 gccaccgtac agctcagcgg agcggacaat tctcccaatg cagtcggcat gctcgtgatc
     1141 agcccggatg ggcagaacgg cagcctggtt gtcgatcaaa tgccggtgct ggaagataat
     1201 caggattacc agctgtggct aatcaaagat ggccagcgca ccagtggagg cgtttttcac
     1261 agctattcca acggctatgg tgtgctgtgg attcatgccg accagccgtt gatcagttac
     1321 gaagaagtgg gagtgacgat tgagccggcc ggaggcagcc ctgccccaac cggtgcaaaa
     1381 ataatcggcg gcttgatcca gaattaggca gcaaaacccg gaacagctcg gtgacccttc
     1441 acctgccgta tacgtatgcc tggcaaaatc cagtgttacg cgagacgatt tacccttatc
     1501 gtcttgaaaa actgcgcgat ttcctgctgg tctatgaaga aattgacctg tgggcgaggc
     1561 tgaaaaatag cccggttgac ccggccctgg aacaggaaat tcgccaggag ctgcccggct
     1621 tacaggccga gctttctact ggtcaggcgc agcaaaagac catccaggca gctttgagtt
     1681 tgctggtcaa gaaattatcc cagattgaac gcctgccgga tgtccgcaag ctggtcctgg
     1741 aaatcgcccg actggaacag gagacggcca gattaaacat ccgccgcgcc ctgctggagg
     1801 gatatatcca gtggtcactc aaaattgccc ccgaaaatga ccccgtccgc ccaaaacgcc
     1861 aggcagagct ggagcaggtc caaacgcagc tgagcgatct tgccagccag atttcaagcc
     1921 tgaagcaaaa ctacgccgaa gtattcctgc ccctgaaaga acaagaagat aaactcgaag
     1981 ccagcgcaga tgagctcgca gagcgcatcg ctgacctgct ggatcgtttg cgtggtattc
     2041 ccctgttttc aacagcccag cctgttaccc ccaaaatcgc tgcccgctgg gcgctcaata
     2101 agtaccgcca gcagctggag gggcttaatc aggaagagct gctggcgctg gtcatggagc
     2161 gcttccaacg ccagccggag cgctacccgg gttggctgca gtacatggtg atccattttt
     2221 cgggcatgcg ctacaaatca gcccatggct cgtgggccga tccacgcctg ctgctccaaa
     2281 aaatccgggc cgacgagctt gaagagcagc tcaattcagc cagtgaggcg cacctgcagc
//
LOCUS       DATMXK010000476         2924 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_97400, whole genome shotgun sequence.
ACCESSION   DATMXK010000476 DATMXK010000000
VERSION     DATMXK010000476.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2924)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2924)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2924
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97400"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..560
                     /locus_tag="VN363_08735"
     CDS             <1..560
                     /locus_tag="VN363_08735"
                     /inference="COORDINATES: protein motif:HMM:NF023045.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="FixG Ig-like domain-containing protein"
                     /protein_id="HWQ84638.1"
                     /translation="QILPAAVSLPAGAAGSFEVQVTIPAGASGSESDTATITATSQGD
                     PAQSDSAQIVTAVTPFYDLSLSSGSAKSGTAGSQVIYSLTITNTGNLTDSFTLAATGQ
                     TWNTTLSTSLVMLAPAASAVFTVTVSIPPGAADQDADSVTITATSQNDGGQSRTAVLT
                     TTTVSSSANPSLFLPVVTANVRPGG"
     gene            727..2805
                     /locus_tag="VN363_08740"
     CDS             727..2805
                     /locus_tag="VN363_08740"
                     /inference="COORDINATES: protein motif:HMM:NF019351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005507 - copper ion binding [Evidence
                     IEA];
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="multicopper oxidase domain-containing protein"
                     /protein_id="HWQ84639.1"
                     /translation="MITRRDFLKLSGAGLLSLYAASHGKYLPRAFASIPGGTLDPDLV
                     PKFITPLLIPPAMPRAATLRMPGGKPVDYYEISMRQITQQILPAGLPPTTVWGYGAIK
                     SANKKGLLIHNAPSLTIEAEYNRPVRVKWINDLVDADGKFLPHLLPVDPTLHWANPPG
                     GIDGRDSRPTFDSTPDLYDGPVPIVSHVHGAVGVGDESDGYPEAWYLPAAKDIPGDYA
                     TEGTWYNYFAGKAAAGYGETWGPGFATFQYPNHNRASTIWYHDHALGMTRLNVYAGPA
                     GFFIVRGGPAGDKAVLDARSGAVAVLPGPAPIEGDHFPPNKTYYEIPIAIQDRAFNSD
                     GSLFYPDTREFFDGATALDPGFIPDTDLSPAWNPEFFGNMIMVNGNTWPFQTVEQRRY
                     RIRFLNGCQSRFLILDFSNIPGVEVWQIGNEGGFLAAPVNITGLGNQLLMGLAERADM
                     IVDFTNVPLGSWTLGNVGPDEPFGGGEPGDDFDVADPDTTGQILQFNVVPALTPDLTT
                     PPQFLILPPIAPLPVEVNTRPLALIEMMSDYWDGPAEAVLGTVDAEGFHHHSMWSEPI
                     TENPAVGDTEVWEFYNFTADAHPMHVHEVVFEVVNREMLVQNDDGESVQPAQLTGIIR
                     GPEPWEVGLKDTVIAYPGEVTRIKATFGTPGTFVWHCHIVEHEDNEMMRPYRIGPADP
                     MAPGAPPMEM"
BASE COUNT          591 a          907 c          830 g          596 t
ORIGIN      
        1 cccagattct gcccgccgcg gtgagcctgc cggccggagc tgcaggcagc ttcgaggtcc
       61 aggtgacaat tccagccggc gcatcaggca gcgagagcga caccgcgacc attactgcca
      121 cctcccaggg tgatcctgcc cagagcgaca gcgcccagat cgtgacggca gtcacaccct
      181 tctatgacct cagcctttca tcgggttcag caaagtcagg taccgcggga tcgcaggtca
      241 tttactcatt gacgatcacc aataccggca acctgaccga cagctttact ctggctgcca
      301 ccgggcaaac ctggaacacg accctgtcca catccctggt gatgctggcg ccggcggcca
      361 gcgcggtctt taccgtcacg gtgagcattc cacccggcgc ggccgaccag gatgccgact
      421 cggtgacgat cactgccact tcccagaacg acgggggtca gagccgtacc gccgttctga
      481 ccacaacgac agtctcatcc agcgcgaatc caagcctgtt tctgcctgtg gttactgcga
      541 atgtacgtcc agggggctga atcttaggct gctggttcag ggagaattct cagctgcagg
      601 tagattgctg agcgggaggt aaatatggta tgaaaaacgc atttattctg aaatcaggca
      661 atcgatccgt gccgttaagg ctaaaagtca taatcgagca gaatgtatta ttagattgag
      721 gccaaaatga ttaccagacg tgattttcta aaactcagtg gtgcaggctt actctcattg
      781 tatgctgcct cccacggcaa atacctgccc agggcatttg catcgatccc cgggggaacg
      841 cttgatcccg atctagttcc caaatttatc acccccttgt taattccacc ggccatgccg
      901 cgcgctgcca cgctcaggat gcccgggggc aagccggtgg actattacga gatatcgatg
      961 aggcagatta cccagcagat cttacctgcc gggctgcccc cgaccactgt gtggggctat
     1021 ggcgcgatca aatcggcgaa taagaaaggc ttgctgatcc ataatgcgcc ttcgctgacg
     1081 atcgaagccg aatacaaccg gccggtgcgc gtgaaatgga tcaatgatct ggtggacgcc
     1141 gatggaaaat tccttccgca cctgctgccc gtcgatccga ccctgcactg ggctaacccg
     1201 cccggcggta tcgatggccg cgactctcgc ccgaccttcg acagcacgcc cgatctctat
     1261 gacggccccg tcccgatcgt cagccacgtc cacggcgcgg ttggggtggg cgatgaaagc
     1321 gatggctatc ctgaagcgtg gtatctcccg gctgcaaaag acatccccgg tgactatgcc
     1381 acggaaggca cctggtacaa ctacttcgcc gggaaggccg cggcgggtta tggtgagacc
     1441 tgggggccgg gctttgccac cttccagtac ccgaaccaca accgcgcttc gacgatctgg
     1501 tatcacgacc acgccctggg gatgacccgc ctgaatgtgt atgccggtcc ggctggcttc
     1561 ttcatcgtgc gcggcgggcc ggccggtgac aaggctgtgc tcgacgcgcg ctctggcgcc
     1621 gtggcagtcc tgccgggtcc ggcgcccatt gagggcgacc atttcccgcc caacaaaact
     1681 tactatgaga tcccaattgc catccaggac cgggccttca actccgacgg ctcgctgttc
     1741 tacccggaca cccgcgaatt cttcgatggc gctaccgccc tggaccccgg ctttatcccc
     1801 gacaccgacc tgtcaccggc ctggaatccc gagttcttcg gcaatatgat catggttaac
     1861 ggcaatacct ggcccttcca gaccgtggag cagcgccgct accgcatccg cttcctgaac
     1921 ggctgccagt cccgcttcct gatcctggat ttcagcaata tccccggtgt ggaagtgtgg
     1981 cagatcggca acgagggcgg cttcctggct gcgccggtga atatcaccgg gctgggcaac
     2041 cagcttctga tgggcttggc ggaacgggcg gatatgatcg tcgacttcac caatgtacct
     2101 cttggcagct ggactctggg caatgtcggc ccggatgagc cgttcggcgg tggtgaaccc
     2161 ggggatgact tcgatgtagc cgacccggat accaccggcc agatcctgca gttcaacgtt
     2221 gtcccggccc tgactccgga tctcaccacg ccgcctcagt tcctgatcct gccgccgatt
     2281 gctccgcttc ccgttgaggt gaacacgcga cccctggcgt tgatcgagat gatgtcggat
     2341 tactgggacg gacccgccga ggctgtgctg ggcactgtgg atgcggaggg tttccatcat
     2401 cactcaatgt ggtccgagcc gatcactgag aatccggctg tcggcgacac tgaagtctgg
     2461 gagttctaca acttcaccgc cgatgcccac cccatgcatg tccatgaggt cgtgtttgag
     2521 gtcgtgaacc gggagatgtt agtccaaaac gatgatggtg aaagcgttca acctgcccag
     2581 ctgactggga taattcgagg cccggagccc tgggaggtgg gtctcaagga taccgtgatc
     2641 gcctatcccg gcgaagtcac ccgcatcaag gccacttttg gcaccccggg taccttcgtc
     2701 tggcactgcc acatcgtcga gcacgaagac aacgagatga tgcgccccta ccggattgga
     2761 ccggcagacc cgatggctcc aggcgctccg ccgatggaga tgtgaccaac taccggaaca
     2821 tctactgcag agtaccggaa taacaatctg gaggggatag aaaatgtata aaaatttgtt
     2881 ccccctgctc gcattggtcg ctgtttgcgt gggatttctg ttcc
//
LOCUS       DATMXK010000477         3247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_4486, whole genome shotgun sequence.
ACCESSION   DATMXK010000477 DATMXK010000000
VERSION     DATMXK010000477.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4486"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..283)
                     /locus_tag="VN363_08745"
     CDS             complement(<1..283)
                     /locus_tag="VN363_08745"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84640.1"
                     /translation="MRKRTFIIGVFALLAACLLAVVVYNIPFVHERLAWRVENLQTQV
                     RHALNPPEQAVFIPQQNDQATMEAIVQATVQAALATPTPTQTSLVLAGAG"
     gene            complement(369..1526)
                     /gene="queG"
                     /locus_tag="VN363_08750"
     CDS             complement(369..1526)
                     /gene="queG"
                     /locus_tag="VN363_08750"
                     /EC_number="1.17.99.6"
                     /inference="COORDINATES: protein motif:HMM:TIGR00276.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:0052693 - epoxyqueuosine reductase
                     activity [Evidence IEA];
                     GO_process: GO:0008616 - queuosine biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA epoxyqueuosine(34) reductase QueG"
                     /protein_id="HWQ84641.1"
                     /translation="MSQPAEVLAAAIKAEAQRMGFQLVGITTPDPPPHLGVYENWLAQ
                     GRQASLDYMASERARQRRADPRLILPECRSILVLGIRYFAPQSNPMPDVEKPELTGRL
                     AAYAWGSDYHTVLVERMQALMAYIADQVGHPVVNRIYTDTGPILEREMAQRAGLGWIG
                     KNTCLIHPRQGSYFLLAEVLLDLELPPDDPFETDHCGSCRRCLEACPTQCILPDRTLD
                     AGRCISYLTIELKEQIPPDLRSLVGNWVFGCDICQQVCPWNQRFAGMEGDPAFAPRTD
                     ATEVQLLAELRLTAQEFNRKFKLSPVQRARRRGYLRNVAVALGNTRDERAGPRLGEVL
                     ANEGDALVRRHAAWALGNIGCEATREYLVSARSNESDPEVLAEIDAALNRL"
     gene            complement(1542..2564)
                     /locus_tag="VN363_08755"
     CDS             complement(1542..2564)
                     /locus_tag="VN363_08755"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HWQ84642.1"
                     /translation="MIKQKVFVLVVALAVVLTACKSPAPETTSEPTKDPNAVMTAAAN
                     TASARLTDIAALTPTALPATPTETPVPATPTLAATTAAPQASPTTAPVTGQDQAAYVS
                     DVTVPDGTNFQPGEQFTKTWRIANTGTSTWTTAYKLAWVDGNQMGGPASVAVPSQVGP
                     GQNVDISVNLTAPATAGKYTGYWKMMNASGALFDSLVYVEINVGGATAAAGTGTPAVT
                     GTPGPSPTTGSNPPTATQAANVVTGAALGVDQGEVSGTCPHTYLFTAQITLSQAANVT
                     YRLEAETGFDLTLPGPATTALNAGVNVMSYQLEFADNVSGWARLRVTSPNEVVSNQVN
                     FTLTCP"
     gene            complement(2665..>3247)
                     /locus_tag="VN363_08760"
     CDS             complement(2665..>3247)
                     /locus_tag="VN363_08760"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84643.1"
                     /translation="ASGVGAAQIGSPEVRTPTHTTRPTRSPTVQPSATETEIPVNTAA
                     LEATQSEATQAVTATITPTLRPSATPSPTPTETPTRTPTSTAAAPFVLRTTQRICEQN
                     QPGGLIQVIALDAAEAQIAGVEVIVSWDGGEDHFYTGLQPELGRGYADFDMTEGLVYA
                     VRLAEAGETARELSTFTCTGGFPGSWRVTFQQP"
BASE COUNT          616 a          961 c          977 g          693 t
ORIGIN      
        1 ggccggcacc ggcgagtacc aatgaggttt gggttggcgt gggggttgcc agcgcagcct
       61 ggactgtggc ctggacgatg gcttccatcg tcgcctggtc attttgctgg ggaataaaaa
      121 ccgcctgctc gggcgggttt aaagcgtgcc gcacctgggt ttgtagattt tctacccgcc
      181 atgccagacg ctcgtgtaca aagggaatat tgtacacgac gactgccagc aagcaggctg
      241 ccaggagggc aaaaacaccg ataatgaagg tccgcttacg catgggggca ttatatcacc
      301 ccgccatacc agcccaaacc aggcaagccg ccctgggcga gttggagggt gaggagtcaa
      361 gtcgtctatt acaggcgatt taaggccgcg tcgatctccg ccagcacctc ggggtcgctt
      421 tcattcgacc gggccgatac taaatactcc cgagtcgctt cacagccaat attccccagc
      481 gcccaggctg catggcgtcg caccagggca tcgccttcat ttgccagcac ttcccctaac
      541 cggggcccgg ctcgctcatc ccgagtgtta cccagggcta cagctacatt acgcaaatag
      601 ccgcgccgtc tggcccgctg gaccgggctg agcttgaatt tccggttaaa ctcctgagca
      661 gtcaggcgca gctcagctaa cagctgcact tccgtcgcat ccgtgcgggg tgcaaatgca
      721 ggatccccct ccatcccggc aaagcgctgg ttccacgggc acacttgctg gcagatgtca
      781 cagccaaaga cccaattacc caccaggctg cgcagatccg gcgggatttg ttctttcaat
      841 tcgatcgtca ggtatgaaat gcagcgtccg gcgtccagag tgcgatctgg gagaatgcac
      901 tgcgtggggc aggcttccag gcagcgccgg cagcttccgc agtgatcggt ctcaaaagga
      961 tcatccggtg gcagctccag gtccagcagg acctccgcca gcaggaagta agatccctgc
     1021 cggggatgga tcaggcaggt attttttcca atccaaccca gcccggcgcg ctgcgccatt
     1081 tctcgctcca ggatcggccc ggtgtcggtg tagatccggt taaccacagg atggccgacc
     1141 tggtctgcga tataggccat cagcgcctgc attcgctcca ccagaacagt gtgataatcc
     1201 gatccccagg cataggctgc cagcctgcct gtaagctcag gtttttcgac atcaggcatt
     1261 ggattagact gcggggcgaa atatcggatc cctaaaacca ggatcgagcg gcattctggc
     1321 aggatcaagc gcgggtcagc ccggcgctgg cgcgcccttt cggaggccat gtaatccagg
     1381 cttgcctgcc gtccctgggc cagccagttt tcgtacacgc ccagatgcgg gggtggatca
     1441 ggtgttgtga tccccaccaa ttgaaagccc atgcgctggg cctcggcttt tatagccgcg
     1501 gcgagaacct ctgctggctg gctcattcag gtctggaaga cttatgggca ggtgagggtg
     1561 aaattcacct gatttgaaac cacctcattc ggcgaggtaa cccgcaggcg tgcccagcca
     1621 gaaacattat cggcgaactc cagctggtac gacatcacat tcacaccggc attgagtgcg
     1681 gttgttgccg gaccgggcag agtaagatcg aagccggtct cagcttccag gcgataagtc
     1741 acattggcag cctggctcag cgtgatttgg gcagtaaaca gataggtgtg cgggcaggtc
     1801 ccgctcacct caccctggtc cacccccagg gctgcgccag tgacaacatt tgccgcctgg
     1861 gtcgcggtag gtggattaga gccggtggtt gggctgggtc caggtgtgcc ggtcaccgcg
     1921 ggcgttccgg tccctgcagc tgcagtcgcc ccgcccacgt tgatctctac atagacgaga
     1981 ctatcaaaca acgccccgct ggcattcatc atcttccagt atccggtgta tttgcctgcc
     2041 gtggcagggg cggtcagatt gaccgagatg tccacatttt gccccggtcc cacctgggag
     2101 ggcactgcca cggaagccgg gccgcccatc tggttgccat ccacccaggc cagtttgtaa
     2161 gcggttgtcc aggtgctggt gcctgtattg gcaatccgcc aggtcttggt gaattgctcg
     2221 cccggttgga aattcgtccc atccggtacg gttacatcgg aaacatacgc cgcctgatcc
     2281 tgcccggtga ccggtgctgt ggtagggctg gcttgcgggg cagctgttgt cgctgccagg
     2341 gtcggggtag ccggcaccgg tgtctcagtc ggagtcgccg gtagggcagt gggcgtcaac
     2401 gccgcgatgt cggtcagacg agcgctggcg gtattcgccg cggccgtcat cactgcattt
     2461 ggatctttgg tcggttcgct ggtcgtctcc ggcgccggcg atttacaagc agtcagtacg
     2521 actgccagcg ccaccacgag aacgaaaact ttttgcttta tcacacttac acctcctgtc
     2581 aatttcagtg acaagtatac ccttttaaaa ggcatcagaa tttttcagcc atttcagtat
     2641 ataaaacttt aaattggggg ggtttcaggg ctgctggaaa gtgacgcgcc acgatcccgg
     2701 aaagccgcct gtacaggtaa acgtgctcag ctcgcgggcc gtttctccag cttccgccag
     2761 gcgaacggcg taaaccaggc cttctgtcat atcaaaatcg gcgtagccgc ggcccagctc
     2821 cggctgcaga ccggtataga agtgatcttc accgccatcc caggagacga tcacctcgac
     2881 acccgcaatc tgcgcttccg ccgcatccag ggcgatcacc tggatcaatc ctcccggttg
     2941 gttttgctcg cagatccgct gcgtggtgcg cagcacaaat ggcgccgcgg ctgtgctggt
     3001 gggcgtccgc gttggggtct cggtgggcgt tggactgggg gtagccgatg ggcgcagcgt
     3061 cggtgtgatt gtcgctgtga ctgcctgggt tgcttcactt tgagtcgctt caagcgcagc
     3121 ggtattaacc ggtatctctg tttcggtcgc agaaggttgg actgttggcg accgggttgg
     3181 ccgcgttgta tgggtaggtg tgcgtacctc tggtgaaccg atctgggctg ctcccactcc
     3241 agaagcc
//
LOCUS       DATMXK010000478         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_74092, whole genome shotgun sequence.
ACCESSION   DATMXK010000478 DATMXK010000000
VERSION     DATMXK010000478.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74092"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..480
                     /locus_tag="VN363_08765"
     CDS             <1..480
                     /locus_tag="VN363_08765"
                     /inference="COORDINATES: protein motif:HMM:NF012694.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="type II secretion system F family protein"
                     /protein_id="HWQ84644.1"
                     /translation="NAKASHARRRITQELPEFLQLMAAESASGAGLETVLMRAAQGEG
                     FLPTWLRRVLAIAHGRSLLLARETGAGILMEEAQRSGHPDLISFAIQMGFARQGTQVQ
                     ETLTRLATQFSDAYIGQAEIRTERLANLLGVLVAVFYFFPFLIVILFVVGTPLMQAF"
     gene            524..742
                     /locus_tag="VN363_08770"
     CDS             524..742
                     /locus_tag="VN363_08770"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84645.1"
                     /translation="MFRKITTFPATLSEKVTRARQFLRSPRSERGEESASVAAITGLV
                     LLIILVIMAIFRNALIATFERIAGLLSF"
     gene            761..1246
                     /locus_tag="VN363_08775"
     CDS             761..1246
                     /locus_tag="VN363_08775"
                     /inference="COORDINATES: protein motif:HMM:NF019428.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TadE family protein"
                     /protein_id="HWQ84646.1"
                     /translation="MNRLIQDITAKTQRSHRLYRFSWRPWRLGGKNLPERGASTVEFA
                     ASVVLVILMFLGMVQFGAYLWASSVVENAARHGARVGSVTQGCAACQAVSAARAALQG
                     QPLLQNPSVTVLAPGGVVGSTVNIRVSARIPLFLPASNSLGLDRLTGVQAEATFRQEG
                     W"
     gene            1250..1687
                     /locus_tag="VN363_08780"
     CDS             1250..1687
                     /locus_tag="VN363_08780"
                     /inference="COORDINATES: protein motif:HMM:NF024792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pilus assembly protein TadG-related protein"
                     /protein_id="HWQ84647.1"
                     /translation="MRKTPERGQMMPFFIFIIMALGLMTAGGLQIGRIVYARGEVGKA
                     ADAAALAAAARLDLVTYRETGQIIFLPDAAVTAQNYASRNAGFLAGRGIGVSVTQIWI
                     DAAARLVFVTVAADLSSLLPGFLEQGGQYQVTGYARARIQATP"
     gene            1828..>2071
                     /locus_tag="VN363_08785"
     CDS             1828..>2071
                     /locus_tag="VN363_08785"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84648.1"
                     /translation="MINQVTIEGFIVSRWNYKGDEFLRIAHHRPRRKGELIHSDYVTV
                     RVAEKTDVLPDLQQGDLVRVLGEVWGKDILEPLGKVF"
BASE COUNT          404 a          596 c          608 g          463 t
ORIGIN      
        1 aacgccaaag cctctcacgc tcgacggcgg ataacccagg aactccccga gtttttacag
       61 ctgatggcag ccgaatcggc cagtggcgcc gggcttgaaa ctgtcctgat gcgtgctgcg
      121 cagggagaag gcttcctgcc gacctggctg cggcgtgtgc tggccatcgc tcatgggcgc
      181 agcctgctgc tggcgcgtga gaccggagct ggcatcctga tggaagaagc ccagcgcagc
      241 gggcatccag atctgatcag ctttgccatc cagatgggct ttgcccgcca gggcacacag
      301 gtccaggaga ccctgacccg tctggcgacc caattttcgg acgcttatat cgggcaggct
      361 gagattcgca ccgagcgcct ggccaatctg ttaggcgttc tggtagccgt gttttacttc
      421 tttccctttt tgatcgttat tctgtttgtt gttggcacgc ctttgatgca ggctttttga
      481 aggcgaccta tccatctaca agttcattca taggaggttc cgcatgtttc gcaaaatcac
      541 aacctttccc gctaccttgt ctgagaaagt cacccgagcg cgccaattct tacgctcccc
      601 gcgcagtgag cgtggcgaag aatcggccag tgtggcagcc atcaccggtt tggtgctgct
      661 gatcatcctg gtgattatgg cgatcttccg caatgccttg atcgctacct ttgagcgcat
      721 tgctgggctg ttaagtttct agcataattc ggaggcacgt atgaatagat tgatacaaga
      781 tataaccgcc aagacgcaaa gatcgcatag gttatataga ttttcttggc gtccttggcg
      841 tcttggcggt aaaaacctcc ccgagcgcgg cgccagcacg gttgaattcg ctgccagcgt
      901 ggtcctggtg atcctgatgt tcctgggcat ggtccagttc ggagcctacc tgtgggcgtc
      961 ctcggtagtc gagaacgctg cccggcatgg ggcgcgggtc ggctcggtca ctcagggctg
     1021 cgctgcatgc caggcggtca gcgctgccag agccgctctg caaggccagc cactgctgca
     1081 aaatccatcg gtcacagtat tggctccagg cggtgtggtc ggctcgacgg ttaatatccg
     1141 cgtctcagcc cgtatcccgc ttttcctgcc ggctagtaac agcctgggtc tggaccggct
     1201 gaccggggtg caggccgaag cgactttccg ccaggaaggc tggtaagaga tgcgtaagac
     1261 gcctgaacgc ggccagatga tgcctttctt catcttcatc atcatggcat tggggctgat
     1321 gaccgccggt ggattgcaga tcgggcgtat cgtctatgcc cgcggcgagg taggcaaggc
     1381 agccgacgcg gcagccctgg cagccgccgc ccggctggat ctggtcacct atcgcgagac
     1441 agggcagatc atttttctgc cggatgccgc ggtcaccgcc cagaattatg cttctcgcaa
     1501 tgccggcttc ctggctgggc gcggcatcgg cgtgtcagtg acccagatct ggattgacgc
     1561 cgcggcgcgc ctggtcttcg tcactgttgc agccgatctc agctcgctct tacccggctt
     1621 tctggaacaa ggcggccagt atcaggtcac cggctatgcc cgggcgcgca tacaagccac
     1681 accttgagga gagtccaatc aactgacctg atctgcccct aaaacaactg catacttcct
     1741 tagaatgtac cgccacacgt gagcagcaac cccgctggat gtgtggcgat tctttttaac
     1801 cgtaagtgaa ggataggagc acagatcatg atcaatcagg tgaccatcga agggtttatc
     1861 gtcagccgct ggaactacaa gggcgatgaa tttctgcgca ttgcacacca ccgaccgcgc
     1921 cgtaaaggcg aactgatcca ttcggactat gtcactgtgc gcgtggcaga aaagactgac
     1981 gtgctaccag acctgcagca aggcgacctg gtgcgcgtgc tgggcgaggt atggggcaag
     2041 gatattctgg agcctctagg caaagtattc c
//
LOCUS       DATMXK010000479         2949 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_4566, whole genome shotgun sequence.
ACCESSION   DATMXK010000479 DATMXK010000000
VERSION     DATMXK010000479.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2949)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2949)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2949
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4566"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(152..2056)
                     /gene="dnaK"
                     /locus_tag="VN363_08790"
     CDS             complement(152..2056)
                     /gene="dnaK"
                     /locus_tag="VN363_08790"
                     /inference="COORDINATES: protein motif:HMM:NF001413.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006457 - protein folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molecular chaperone DnaK"
                     /protein_id="HWQ84649.1"
                     /translation="MAKIIGIDLGTTNSVVAVMEGGDPTVIQTAEGSRLLPSVVGFNK
                     NGERMVGQTAKRQAVVNAENTVFSIKRFMGRHYDEVGTEQKMVPFQIVSGPSGDARVK
                     IPVTSREYSPQEISAMILAKLKTDAEAYLGEPVTKAVITVPAYFNDSQRQATKDAGRI
                     AGLEVLRIINEPTAAALAYGLDKQKNETILVFDLGGGTFDVSVLDVGEGVIEVRATNG
                     DTHLGGDDWDEKIVDWVASEFQKEQGIDLRQDRQALQRLREAAEKAKIELSSVMESEI
                     NLPYITADASGPKHLQIKLSRSKFEQLTEDLVKRLRGPFEAALRDANLSASDIHQVVL
                     VGGATRMPMVQELVRSITKKEPHKGVNPDEVVAIGAAIQAGVLAGDIKDVLLLDVTPL
                     SLGLETLGGVMTTLIERNTTIPVKKTEVFSTAEDSQTAVDVHVIQGERPMSGDNMSLG
                     RFRLEGIPPAPRGMPQIEVTFDIDANGILNVTARDKASGKEQKVTITASTNLNKEEID
                     RMVRQAREHESEDRQRRELIEARNNADSLAYQTEKTLRDLGDKIPGNEQQNIEQKINQ
                     LREAIKGDDIQHIRRLSDELQNAFHAISQQMYAQQAATAGPGPVPGPNSGPEPRNDDG
                     DVIEGEYREA"
     gene            2687..>2949
                     /locus_tag="VN363_08795"
     CDS             2687..>2949
                     /locus_tag="VN363_08795"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560310.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HWQ84650.1"
                     /translation="MKAEQAWQAAVGQLQMEMPKAAFETWVSSTEFLSYEDGSFTVGV
                     QNAYARDWLESRLSSTVTRILTGIMNRTVEVHFRTWQKPAETET"
BASE COUNT          661 a          736 c          749 g          803 t
ORIGIN      
        1 tctctgaatg gtttaaggtt attggacagg tccattatac aatgattttt ctaatgtctc
       61 ggtgaggcat ggcgtatagc ttgcgatcga tattaaaaag cccgccccga attcgtttcg
      121 gggcgggcca ggttacacaa gaaaaagctt atcaggcttc gcgatattcg ccttcgatca
      181 catcaccgtc gtcattacgc ggttctggac cggaattggg gccaggtacc gggccagggc
      241 cagcggtagc tgcctgttga gcatacatct gctggctgat ggcgtggaag gcgttctgca
      301 actcatcact gaggcgacgg atatgctgaa tgtcatcgcc ctttatggct tcacgcagct
      361 gattgatttt ctgctcgatg ttttgctgct cattacccgg gattttatct cccagatccc
      421 gcagcgtttt ctcggtctga tatgccaggc tgtcggcatt gttgcgggcc tcgataagct
      481 cgcgccgctg ccggtcttct gattcgtgct cgcgagcctg gcggaccata cgatcaatct
      541 cttccttgtt caggttggtc gaagcggtaa tggtcacctt ttgctctttc ccactggctt
      601 tatcccgggc agtcacattt aagatgccat tggcgtcaat atcgaaagta acttcgatct
      661 gcggcatgcc gcgcggcgca ggtgggatac cttccaggcg gaagcgccct aaactcatat
      721 tgtcgccaga catgggccgt tcaccctgga ttacatgcac atccacagca gtctggctgt
      781 cttcagcggt ggagaatact tccgttttct tcacggggat cgtggtattc cgttcaataa
      841 gcgtggtcat tacgccccca agggtttcca ggcccaggga gagaggagtg acatccagca
      901 ggagcacatc cttgatatca ccggccagaa cacctgcctg aatagctgct ccgatagcga
      961 caacctcatc cgggtttacc cctttatggg gttctttctt ggtaatcgaa cgcaccaatt
     1021 cctgaaccat gggcatgcgg gtagcaccgc caaccaggac tacctggtgg atatcgctgg
     1081 cgctcaagtt cgcatctcgc agggctgcct caaatggacc acgcaagcgc ttaactaagt
     1141 cttccgtaag ctgttcgaat ttcgaacggg aaagcttgat ctgcaaatgt tttggtccag
     1201 aagcatccgc cgtaatgtag ggcaggttaa tctcgctttc catcacgctc gaaagctcaa
     1261 tcttggcttt ttctgcagcc tcgcgcaggc gctggagcgc ctggcgatcc tggcggagat
     1321 cgataccctg ctccttttgg aattcgctgg caacccagtc tacaatcttc tcatcccagt
     1381 catccccgcc cagatgggta tcaccgttcg tggcgcggac ttcgataaca ccttcaccaa
     1441 catccaggac actgacatcg aatgtgccac ctccgaggtc aaacaccagg atggtctcat
     1501 ttttctgctt gtcgagtcca tatgccagag cggctgcggt aggctcattg ataatccgca
     1561 gaacttcgag accagcaatt cggccggcat ccttcgttgc ctggcgctgg ctatcgttga
     1621 agtaggccgg gacggtgatt accgctttgg taaccggttc tcccagataa gcttcggcat
     1681 cggttttgag tttagcgagg atcattgcag aaatttcttg tgggctgtat tcgcgactgg
     1741 tgacggggat ttttacccgt gcgtcaccag acggaccgct cacaatctgg aatggaacca
     1801 ttttttgttc ggttccaact tcgtcataat gccgccccat gaaccgtttg atagagaaca
     1861 cggtattctc cgcgttgacc actgcctggc gtttggctgt ctgtccgacc atacgttcgc
     1921 cgtttttgtt gaaaccaact acggaaggca gtaatcgaga accctccgca gtttgtatca
     1981 ctgttggatc gccgccttcc atgacggcaa cgacactgtt agtagttccc aggtcaatac
     2041 caataatctt agccattttt gtatctcctc ttttgctata aggatgtaat ttatgaaatc
     2101 atacagacta agtttagcgc tggtacaata gaagaacgtt aacaacgagt aggaattttg
     2161 taaacacagt tggctggtcc atttaatgct catgggaaga aattctatct cttgtggacg
     2221 actcaccaaa ttgcgattct ccaccccctg tggggaggcg ctctttggaa ttcaaatctt
     2281 taaggtttat acaccccaca tatcgacttg agcctataag gggcacatta tcggctatat
     2341 atgggggtcg cgtcggttaa gagccagata ttagggttac acgagggatg gaatcttgcc
     2401 tctcaaaggt tagtaaagca ttgcaaaatt taagaatccc taagcttaaa gtattggttt
     2461 tttttagact agcgtagggt tttagtattg tggagatagg gggttttctg ctagaatact
     2521 aaggcaagtg gcaaaagtga atagcttcag ggggctcctt cgaacccatt ggcaatgggt
     2581 atcgtctaaa ttataccttg ttcgttattt tccctgttgc gttcgcaaca ggtgagttgt
     2641 ttctgcatgg tttgtgccaa atttatgtat tgaggagaag gccgtcatga aagctgaaca
     2701 ggcctggcaa gctgccgtcg gacaattgca gatggaaatg ccaaaggctg cctttgaaac
     2761 ctgggtgagc tctactgaat tcctctctta cgaggatggt agttttaccg ttggcgttca
     2821 gaacgcttat gcacgcgact ggctggagag ccgcttatcc agcacggtta ctcgtatttt
     2881 gaccgggatc atgaatcgaa ctgttgaagt gcattttaga acctggcaaa aaccggccga
     2941 gactgagac
//
LOCUS       DATMXK010000480         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_62328, whole genome shotgun sequence.
ACCESSION   DATMXK010000480 DATMXK010000000
VERSION     DATMXK010000480.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62328"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1187
                     /locus_tag="VN363_08800"
     CDS             <1..1187
                     /locus_tag="VN363_08800"
                     /inference="COORDINATES: protein motif:HMM:NF023802.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="C39 family peptidase"
                     /protein_id="HWQ84651.1"
                     /translation="VGILYWLPPINRRLSWRVDLAESYLRGVIDPVEELPTSLPQPRV
                     VVNSRPTETPLPPAVLSPTAGPSPTPTLSPTPLPEKVSLTAPAWEKQDINNCGPATLA
                     MNLRYYGWEGDQMEIAELLKPVRQDRNVNVEELIYYVRTRAGWLNAEFRVGGDLELLK
                     QLLAAGIPVMIEESFHFEETYWPNDDQWAAHYNLLTGYDEAAQTFISQDSFYGANQIV
                     SYTKTNEYWKSFNYVYIMFYLPDQEETVRSILGENWDVDINRQNALDKALEQTREDSE
                     DAYAWFNMGTNLVFFGRYAEAAQAYDQARALGLPQRMLRYQFGPFFAYFNTGRSDDLQ
                     AMIDYALARTPNAEEALLWRGWANYQKGDVREAEASFRQALEENPNYQDAKLALDILR
                     ND"
     gene            1253..1864
                     /locus_tag="VN363_08805"
     CDS             1253..1864
                     /locus_tag="VN363_08805"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560165.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="TIGR00730 family Rossman fold protein"
                     /protein_id="HWQ84652.1"
                     /translation="MAIATICVYCGSADHLHPEYLDSARRMGEVIAMRKIRLVFGAGA
                     TGLMGAVADGALEAGGEVTGVIPEYFNTPQLCHPRLTHLEVVETIHLRKARMAELADA
                     FIALPGGFGTFEEFFEILTWAQIGLHQKPIGLLNVRGYFNPLLNMIDHARDEGFIYNE
                     HSTLFVHTRQPEDLLTALEKFESPGGLDRWLLRPDENSLASQP"
BASE COUNT          482 a          504 c          534 g          499 t
ORIGIN      
        1 tcgtcggtat attatactgg cttcctccga taaatcgccg tttatcctgg cgggtagatt
       61 tagctgaatc gtacctgcgg ggagtaattg acccggtcga agagctgcca acgtcactcc
      121 cccaaccgcg cgtggtagta aacagccggc ccaccgaaac acctctgcct cctgctgttc
      181 tgtctcccac agccggccca agcccgacgc ctacactttc accgacccca ttaccggaaa
      241 aagtcagcct gactgcccca gcctgggaaa aacaggacat caataattgt ggaccagcta
      301 ccctggccat gaatctacgt tattatgggt gggaaggcga ccagatggag atagccgaat
      361 tacttaagcc tgtccgccag gaccggaacg ttaatgttga ggagttgatc tattatgtac
      421 gtacccgggc tggatggctc aatgcagagt ttcgagtggg cggtgatctg gaactattga
      481 agcaactcct tgccgctggg atacccgtga tgatcgagga gagtttccac tttgaggaaa
      541 cctactggcc taatgatgat caatgggcgg ctcactacaa tttgttgaca ggttatgatg
      601 aggctgctca gacgttcata tcccaggata gtttttacgg agccaaccag atcgtcagct
      661 acacaaaaac caatgagtac tggaagtcct tcaattacgt ttatattatg ttttacctgc
      721 ccgatcagga agaaacggta cgcagtatcc tgggtgaaaa ttgggatgta gacatcaacc
      781 gccagaatgc actcgataaa gcgcttgagc aaactcgaga ggattccgag gatgcttatg
      841 cctggtttaa tatgggcacc aatctggtgt tctttgggcg ctatgccgaa gccgctcagg
      901 cgtatgacca ggcgcgggcg cttggcttac cgcagcgaat gctgcgttac cagtttggtc
      961 cattcttcgc ttactttaat accgggcgaa gcgatgatct tcaagcgatg atcgattatg
     1021 cgctggcacg cacacccaat gctgaagaag cattattgtg gcggggttgg gcgaattacc
     1081 agaaggggga tgttcgtgaa gccgaagcca gcttccgcca ggctctcgag gaaaacccga
     1141 attatcagga tgcaaagctg gcgttggata ttttacgcaa cgattagcct tttttgcgga
     1201 tgaaatcccg agctggaagc aggctcatct ttcgttattt gtagggaaca aaatggcgat
     1261 tgcaacgatt tgtgtttatt gtggttcagc tgatcatctc catccagaat atctggacag
     1321 cgctcggcga atgggggagg tgattgccat gcgaaagatc cgcctggttt ttggtgcagg
     1381 ggcaactgga ttaatggggg ctgtggctga cggagccctg gaagcgggtg gcgaagtgac
     1441 cggcgtaatc ccggaatatt ttaatacgcc acagctgtgc catccccggc tgacccacct
     1501 ggaggttgtc gagaccattc acctccgcaa ggcgcgtatg gcagagctgg cagatgcatt
     1561 catcgcctta ccaggcgggt tcggcacttt tgaagagttt tttgagattc ttacctgggc
     1621 ccagattggg ctgcatcaaa aacccattgg tttgttgaat gtgcgcggat attttaaccc
     1681 tctgctaaat atgatcgacc acgcccggga cgaaggattt atttataacg agcacagcac
     1741 attatttgtc catacccgcc aaccggaaga tctgctcacc gctctggaga aatttgaatc
     1801 accgggtggt cttgatcgat ggctgctgcg cccggatgaa aacagccttg catctcagcc
     1861 atgagcgtgc tataatcaat gtacgtaaca aacagtcggc tatgcgttaa tgcgactgaa
     1921 tcccccttat tcggtgttca atatctgact tttacccctc tccgagtatt ccccggcgtg
     1981 ttctgcgaat tagtaaccgc tggccagctg ccccagtac
//
LOCUS       DATMXK010000481         2242 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_4678, whole genome shotgun sequence.
ACCESSION   DATMXK010000481 DATMXK010000000
VERSION     DATMXK010000481.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2242)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2242)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2242
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4678"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            242..613
                     /locus_tag="VN363_08810"
     CDS             242..613
                     /locus_tag="VN363_08810"
                     /inference="COORDINATES: protein motif:HMM:NF013098.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="VOC family protein"
                     /protein_id="HWQ84653.1"
                     /translation="MELGAFSISLAVKDIHASKAFYEKLGFQEFEGDISQNWLILKNG
                     ATVIGLFQGMFEKNILTFNPGWDQDAQELDSFTDVRELQRQLIGRGVTLLSEADGTTQ
                     GPASLMLLDPDGNTILIDQHR"
     gene            674..832
                     /locus_tag="VN363_08815"
     CDS             674..832
                     /locus_tag="VN363_08815"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84654.1"
                     /translation="MARTQRRSGSFLIGLYAWVAAVLFGMVVGDIVYARLAPGSAAAF
                     SAISDLLL"
     gene            complement(886..1071)
                     /locus_tag="VN363_08820"
     CDS             complement(886..1071)
                     /locus_tag="VN363_08820"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84655.1"
                     /translation="MVQSLLNPFANIEASPVGMSLSQGPAAYTAELASAAGKKTGIAN
                     SSSSTSRLASRRLRAGA"
     gene            1101..2081
                     /locus_tag="VN363_08825"
     CDS             1101..2081
                     /locus_tag="VN363_08825"
                     /inference="COORDINATES: protein motif:HMM:NF039905.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016301 - kinase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="diacylglycerol kinase family protein"
                     /protein_id="HWQ84656.1"
                     /translation="MKENQTTPPPSNPYRSRRAKLIYNPGAGTAKESPLNLQDVVQEL
                     QQWKLVPETYLIEPDGNLPEMVADALARGIRLFVVCGGDGTVSTVSHILAGTRATLGI
                     IPLGTQNNTALSLGIPEDIPAAVALLRTGKRIKVDMGVVTCAGNTTPFMEVCSVGLVS
                     DLFPSADDIQHGNLLRVGDFLATLAAAPPAEIHLNLDGKLDLNSTGYVVLATNMPHIG
                     LHYLVSSSAAYDDGLLDVLFFAELSKLDLLGYVLQGVGEGMADDPRVQHFRVRSLDID
                     TQPAMAVMVDGFALGEGPVHIEVRKRSLGVMVAQDHAGPPGQRKAAAADA"
BASE COUNT          424 a          684 c          694 g          440 t
ORIGIN      
        1 cacaatttat ccgattagcc acaggtgagc gcaaaattac ccggaaaatg cccttattta
       61 ttgaccgtcc ggatggattt gcgcgccttg acgctggaga ctgccccgat tatgatcgaa
      121 acatcccgcg gggccggtaa atcgtacttc cccgctcggt ttttccaccc cagcccgtta
      181 atcgaacggg tgtgagcaaa aaacaattaa cctggatatt caggacagac aggagaatcc
      241 tatggaactg ggtgcatttt caatcagcct ggcagtcaaa gatatccacg cttcaaaagc
      301 attttacgag aagctgggat ttcaggagtt cgagggagat atcagccaga actggctgat
      361 cctcaagaac ggggcaacgg tgatcgggct gttccagggg atgttcgaga aaaatatcct
      421 gaccttcaat cccggctggg accaggatgc gcaagagctc gattccttca ccgatgtgcg
      481 cgagctgcag cgccagctca ttgggcgggg tgtgacgctg ttaagcgagg cggatggaac
      541 cacccagggc ccggcgagcc tcatgctgct ggacccggat gggaatacca tcctgatcga
      601 ccagcaccgc tgaggccggg tggatccggc gggctgaacc cgcaacctgc cccctgcgaa
      661 tggagataag ctcatggcca gaactcagag gcgatccggt tctttcttaa tcgggttgta
      721 tgcctgggtg gcggcggtcc tgtttgggat ggtggtgggc gacatcgtgt acgcccggct
      781 ggcaccgggc agtgcagccg cattttcagc catctcggat ttgctgttgt agaccggcgc
      841 cttcgcaatg ctatgcgcag tcatcgccat cgccgctgcc tggaatcacg cccccgcccg
      901 cagtctcctg ctcgccagcc tgctggtgct gctgctggag tttgcgatcc cggttttctt
      961 gccggcggcg ctcgccagct cagcggtata ggctgccggt ccctggctca ggctcatccc
     1021 caccgggctg gcttcgatat tggcgaatgg attcagcaaa ctctggacca ttcgggacgt
     1081 tttcaggtat gatgtggctt atgaaagaaa accagactac cccacccccc tccaaccctt
     1141 accgcagccg gcgagccaag ctgatctaca accccggcgc cggaacggcg aaagaatccc
     1201 cgctcaattt gcaggatgtc gttcaagagc tgcagcagtg gaagctggtg cccgaaacct
     1261 acctgatcga gcccgacggc aacctcccgg agatggtggc cgatgccctg gcgcggggga
     1321 tccgcctgtt cgtggtgtgc ggcggcgacg gcaccgtttc cacagtatcc catattctgg
     1381 ccggcacccg cgccaccctg gggatcatcc cgctgggcac ccagaacaat accgccctca
     1441 gcctgggcat cccggaggat atcccggccg cggtggccct cctgcgcaca gggaaacgga
     1501 tcaaagtgga tatgggcgtg gtcacctgcg ccggaaatac caccccattc atggaagtct
     1561 gctcggtggg gctggtatcg gatctgttcc cctcggccga tgatatccag cacggcaacc
     1621 tgctgcgggt gggcgatttc ctggccaccc tggcagccgc cccacccgcc gagatccacc
     1681 tgaacctgga tggcaaactg gacctgaaca gcacggggta tgtggtgctg gccaccaata
     1741 tgccccacat cggcctgcac tatctggtca gctcctcggc cgcctacgac gatggcttgc
     1801 tggatgtgct gttcttcgcc gagctttcga agctggattt gctgggctat gtgctgcagg
     1861 gggtgggcga aggcatggcc gacgatcccc gcgtccagca tttccgggtg cgcagcctgg
     1921 atatcgatac gcagccagcg atggcggtga tggtggatgg attcgcactg ggggagggtc
     1981 cggtgcacat cgaagtgcgc aagcgctcgc tgggggtgat ggtcgcccag gatcatgccg
     2041 gtccgcccgg gcagcgcaag gcggcagcag ccgatgcgtg agcaggtacc gccggcgcct
     2101 gaggctgcac cacccaagct cgacgggacg gcgagcacgc ctgagggcgc gccgccctgg
     2161 gcgcgatttt ccgtgtggct gaaccagcac cgcacgtctg cccggctgac cctgctgact
     2221 gccgggctgg tcctgtttgt gg
//
LOCUS       DATMXK010000482         2292 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_107697, whole genome shotgun sequence.
ACCESSION   DATMXK010000482 DATMXK010000000
VERSION     DATMXK010000482.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2292)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2292)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2292
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107697"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..519
                     /locus_tag="VN363_08830"
     CDS             <1..519
                     /locus_tag="VN363_08830"
                     /inference="COORDINATES: protein motif:HMM:NF027484.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NBR1-Ig-like domain-containing protein"
                     /protein_id="HWQ84657.1"
                     /translation="VLIITTLVSACSNPSPDQPAPGEGFRPPTQAVSPTAPPATPVIL
                     VTATPDGSIFHPTATPSCSDSLTFLEDLTVPDGTPVTPGAVLDKRWQVQNSGTCNWDA
                     GYTIQRTAGPELGAPQNLALYPARSGGEATLRIEFIAPNEAGTYRSAWQAYNPNSVAF
                     GDPFFIEFVVSP"
     gene            complement(595..1854)
                     /gene="ahcY"
                     /locus_tag="VN363_08835"
     CDS             complement(595..1854)
                     /gene="ahcY"
                     /locus_tag="VN363_08835"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560023.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004013 - adenosylhomocysteinase activity
                     [Evidence IEA];
                     GO_process: GO:0006730 - one-carbon metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="adenosylhomocysteinase"
                     /protein_id="HWQ84658.1"
                     /translation="MENYDVKDTKLAEGGRRKIEWAEREMPVLRMIRERFKKERPLQG
                     LRLSACLHVTSETANLVHTLYEGGADIVLTASNPLSTQDDVAAALVTHFEIPVFAIKG
                     ESNVTYYKHLNAALDHKPNLTMDDGADLVSTLHKDRRDLLPHIIGGTEETTTGVIRLR
                     AMAADGALEFPVMAVNDAMTKHFFDNRYGTGQSTVDGIIRATNILLAGKTVVVAGYGW
                     CGRGFANRARGMGSNVVVTEVDPLPALEAVMDGYRVMPMSEAAKIGDIFVTMTGDLNV
                     IDRHHFEFMKDGAIVANSGHFNVEINIPALEELSVGKRLVRPFVEQYELKDGRQIHLL
                     GEGRLINLASAEGHPASVMDMSFANQALAAEYMVKNADKLENKVYSVPDAIDREIARI
                     KLDAMAVAIDTLTEQQVKYLHSWQEGT"
     gene            complement(1887..>2292)
                     /locus_tag="VN363_08840"
     CDS             complement(1887..>2292)
                     /locus_tag="VN363_08840"
                     /inference="COORDINATES: protein motif:HMM:NF012515.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="PfkB family carbohydrate kinase"
                     /protein_id="HWQ84659.1"
                     /translation="YEFALIQKCTGLAPAEIHQNVGFTVITHGKRGTSIITKQANYEI
                     PVFPEHQIVDPTGVGDAFRGGFLKGYALGLDWETCGRMGALAATYCLEQDGTQGYSFT
                     PREFVARYREYFDDNGKLDVLVNNWVANQVQS"
BASE COUNT          536 a          655 c          561 g          540 t
ORIGIN      
        1 gtgctgatca taactaccct ggtcagcgcc tgcagtaatc catcacccga tcagcctgcc
       61 ccgggagaag gttttcgtcc gccgacccag gcagtcagcc caaccgcgcc tcctgccact
      121 ccagtaatcc tggtcaccgc gacacctgac gggagtattt ttcaccccac cgcaacgcct
      181 agctgcagtg actcattgac cttcctggag gacctgaccg taccggatgg cacgccggtc
      241 actccaggcg ctgtgctgga caaacgctgg caggtgcaaa attccggaac ctgcaattgg
      301 gatgcaggtt atacaatcca gcggactgcc ggcccggagc tcggcgctcc ccaaaacctg
      361 gcactctacc cggctcgcag cggtggtgag gccacattga gaattgaatt catcgctccc
      421 aacgaggctg gaacctatcg cagcgcctgg caggcttata accccaacag tgtggctttc
      481 ggagatccat tctttatcga attcgtcgtc tcgccttgag atcgttctcg gattaaataa
      541 aaccggctgg atccgataaa tgggtccagc cggttatttc ttaatcagaa atttctaagt
      601 accttcttgc caggaatgaa ggtatttgac ctgctgctcg gtaagtgtat caatcgctac
      661 ggccatggca tccagcttga tgcgggcgat ttcgcggtct atggcatccg gcacgctgta
      721 aactttgttt tccagcttat cggcattttt aaccatatat tcagccgcca gggcctgatt
      781 ggcaaaagac atatccatga cactggcagg atgaccttcg gcggaagcca ggttaattaa
      841 gcgaccctca cccagtagat ggatctggcg cccatctttc aattcatatt gctccacaaa
      901 gggacgaacc agacgctttc caacggacaa ttcttcaagt gcagggatat tgatctcgac
      961 gttaaagtgg ccggaattag cgacaattgc gccgtccttc ataaattcga aatggtggcg
     1021 gtcaatcaca ttcaaatccc cggtcatggt gacaaagata tccccgattt tggcagcctc
     1081 agacatcggc atcacccggt agccgtccat cactgcctcc agagctggca gcggatcaac
     1141 ttcggtgacg accacgttgg aacccatgcc ccgtgcccga ttggcaaaac cacgcccaca
     1201 ccagccataa ccagctacga ccaccgtctt gcctgccagc agaatgttgg tcgcccggat
     1261 aatcccatcc acagttgatt ggcctgtgcc gtaacgatta tcaaagaaat gcttggtcat
     1321 ggcatcatta acagccatta ctggaaattc cagcgcgcca tccgcggcca tcgcccgtag
     1381 acggatgacc ccggtggtgg tttcttcagt gccgccaata atgtggggaa gcaggtcacg
     1441 gcgatcctta tgcagggtgc tcaccagatc tgcgccatca tccatggtca ggttaggttt
     1501 gtgatccagg gcagcattga gatgcttgta ataggttacg ttgctctcac ccttgatggc
     1561 gaacaccggg atctcgaagt gggttacaag agctgccgca acgtcatcct gcgtcgagag
     1621 tggattcgaa gctgtcagga caatgtcggc cccaccttca tacagggtgt ggaccaggtt
     1681 tgcggtctca ctggttacat gcaggcaggc agaaagccgg agaccctgca aaggacgttc
     1741 ctttttaaaa cgttcccgga tcatacgcag caccggcatc tcgcgctctg cccattcaat
     1801 cttgcgccgg ccgccttcgg ccagcttggt atcttttaca tcatagtttt ccatactaat
     1861 ctccatttga aatgatcagc ttaaattcaa gattggacct gatttgcgac ccaattattt
     1921 acgagcacat ccagcttacc gttgtcgtcg aaatattcgc gatagcgagc tacaaattcc
     1981 cggggagtga aggaataacc ctgggtacca tcctgctcca gacaataggt agctgccaga
     2041 gcacccatcc ggccgcaggt ctcccagtcc agccccaatg catatccttt taagaaacca
     2101 cctcggaagg catcgccaac tcccgtcgga tcaacgatct ggtgttctgg gaagacgggt
     2161 atttcataat tggcctgttt cgtgatgatt gaagtccctc ttttgccatg tgtaatgaca
     2221 gtaaaaccta cattctggtg gatctcggcg ggtgccaatc cggtgcactt ctgaataagg
     2281 gcaaattcat at
//
LOCUS       DATMXK010000483         2369 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_74348, whole genome shotgun sequence.
ACCESSION   DATMXK010000483 DATMXK010000000
VERSION     DATMXK010000483.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2369)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2369)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2369
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74348"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1164)
                     /locus_tag="VN363_08845"
     CDS             complement(<1..1164)
                     /locus_tag="VN363_08845"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84660.1"
                     /translation="MRLTETQRNLVEAPLDQRLFIEGPANSGKTSVGVERLLYLMQEG
                     IPADNILLILPQRTLAGPYQAALQHPGVVSGGIVSLITIGGLARRMVDLFWPYVSRAG
                     GFHNPQKLPVFLTLETAQYYMARIAGSLFAEGLFSTVPLERNRLYSQILDNLNKAAVV
                     GFPYTEISARLKGAWQGDPAQLRVYEDVQTCAAKFREYCLENNLLDFSLQIELLRDQL
                     WPLEEFRQYLFGRYKHLLADNIEEDVPLTHDILLEWLPQASSACLIYDQGAGFRSFLG
                     ADAESAYRLKAACDQTAELAEPWQVNPGIETLREALHHTIQRPLLSLNGQTPPPNILE
                     TAGESEKAYTRLAVSYSYERYYPQMLDWVAGQVAYEVNENLVPPGEIVVLAPFL"
     gene            complement(1185..>2369)
                     /locus_tag="VN363_08850"
     CDS             complement(1185..>2369)
                     /locus_tag="VN363_08850"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561337.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ84661.1"
                     /translation="GIQRALYGVTFFVIIGLLSAVGTAVVYGVGGVLVIRQVFTIGTI
                     VAFGAYLSSLYGALQGLTNAPVEFATSVVSFERVFEVIDLPLDIAETADPHPLPEVNG
                     ELEFDNVNFTYQAPSQSVLSAVARYGSMDNVNTVISGSPPAPGDGKTPEFTPEESPRS
                     QAREQALRGITFKAMPGQLVALVGPSGAGKTTLTYLIPRLYDPTAGVIRIDGWDLRGV
                     SLDSLASQIGMVTQETYLFHDTIRTNLLYANLDATQAEMEAAAKAANIHDFIRELPEG
                     YDTIVGERGYRLSGGEKQRLALARVILKNPRILVLDEATSHLDSESEALIQEALKRVM
                     AGRTSIVIAHRLSTILAADLILVMDRGQIVERGSHSELLALGGLYASLYETQFSREID
                     QA"
BASE COUNT          478 a          680 c          744 g          467 t
ORIGIN      
        1 gaggaagggc gccaggacga caatctctcc cggggggacg agattctcat tgacctcata
       61 ggcgacctgc ccggcgaccc aatccagcat ctgcgggtaa tagcgttcat aggaatagct
      121 caccgccagg cgagtgtagg ctttttcgga ttcaccggcg gtctcaagga tatttggagg
      181 cggcgtctgc ccattcaggc tcagcagcgg tcgctggatg gtgtggtgca aagcttcgcg
      241 cagggtttcg attcctggat tcacctgcca gggttcggcg agctcagcag tctggtcgca
      301 ggcggctttc aagcggtagg cgctctcggc atcggctccc aggaagctgc ggaaacccgc
      361 gccctggtcg tagatcaggc aggcggagct ggcctggggt agccattcaa gcaggatatc
      421 gtgggtcagc gggacatctt cttcgatatt atcggccagc aggtgtttgt agcgcccgaa
      481 aaggtactgg cgaaattcct ccagaggcca gagctggtcg cgcagcagct cgatctgcag
      541 ggaaaagtcg agcagattgt tctcaaggca gtattcacgg aattttgccg cacaggtctg
      601 cacatcctca tagaccctga gctgggcagg atcgccctgc caggcacctt tcaggcgcgc
      661 gctgatctcg gtgtacggga agccgaccac ggcggcttta ttcaggttgt ccaggatctg
      721 cgaatacagc cggttgcgct ccagggggac agtgctgaac agcccttccg caaacaaact
      781 accggcaata cgggccatgt aatactgggc ggtctccagg gtcaaaaata ccggcagttt
      841 ttggggattg tggaatccgc cagcccggga gacatacggc cagaacaggt cgaccatgcg
      901 gcgggccaga cccccgatcg tgatcaggct gactatgccg ccggagacca cccccggatg
      961 ctgcagagca gcctggtagg gtccggcgag ggtgcgctgg gggaggatca acaggatatt
     1021 atctgccgga atgccttcct gcatcaggta cagcaggcgc tccacgccta cactggtttt
     1081 accgctgttg gccggccctt cgataaacag gcgctggtcc aggggggctt caaccaggtt
     1141 acgctgggtt tctgtcaggc gcaaagtgcc tcctttttat ggcgctaagc ctgatctatt
     1201 tcacggctga actgggtctc gtacaggctg gcatacaggc cgcccagggc gagaagctcg
     1261 gaatggctgc cgcgctctac gatctggccg cggtccataa ccaggatcag atcggctgcc
     1321 agaatagtgc tcaggcggtg ggcgatgacg atgctggtgc gcccggccat gacccttttt
     1381 aaagcctcct ggatcagggc ttccgattct gagtccagat ggctggtggc ttcgtccagg
     1441 accaggatgc gcgggttctt caagatcacc cgcgccagcg ccaggcgctg cttctcgccg
     1501 ccgctcaggc ggtagccccg ctcgccgacg atggtgtcgt aaccttccgg cagctcacgg
     1561 atgaaatcgt gaatattggc ggctttcgca gcggcttcca tctccgcctg ggtggcatcc
     1621 aggttggcgt atagcagatt ggtgcggatc gtatcgtgga atagataggt ttcctgggtc
     1681 accatcccga tctgggaggc cagggaatcg agggaaaccc cgcgcaaatc ccagccatcg
     1741 atgcggatca cgccggcggt cgggtcatac aggcgaggga tcaggtaagt cagggtcgtc
     1801 ttgcccgcac cgcttggccc gaccagggct accagctgtc cgggcattgc cttgaaggtg
     1861 atgccgcgca gcgcctgctc gcgggcctgg ctgcgagggc tctcttcggg tgtgaattca
     1921 ggagtcttgc catcccccgg ggcgggcggg ctgcctgaga tgactgtatt gacattatcc
     1981 atactcccat agcgagccac ggcgctcaac acagactgcg aaggcgcctg gtaggtgaag
     2041 ttgacattat caaattccag ctccccattt acttcaggta aaggatgcgg atcggctgtc
     2101 tcagcaatat ccagggggag atcgatgact tcaaaaaccc gctcgaagct gaccaccgac
     2161 gtggcaaatt caaccggggc attggtcaat ccctgcagtg cgccgtagag gctggataga
     2221 tacgctccaa aggctacgat cgtgccgata gtgaacacct ggcggatgac cagcacaccc
     2281 ccgacgccgt aaacgacggc cgtgcctacc gcggagagca agccgatgat gacaaaaaag
     2341 gtaacgccgt acagggcgcg ctggatgcc
//
LOCUS       DATMXK010000484         2338 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_157250, whole genome shotgun sequence.
ACCESSION   DATMXK010000484 DATMXK010000000
VERSION     DATMXK010000484.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2338)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2338)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2338
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_157250"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1160)
                     /gene="secD"
                     /locus_tag="VN363_08855"
     CDS             complement(<1..1160)
                     /gene="secD"
                     /locus_tag="VN363_08855"
                     /inference="COORDINATES: protein motif:HMM:TIGR01129.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0031522 - cell envelope Sec protein
                     transport complex [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="protein translocase subunit SecD"
                     /protein_id="HWQ84662.1"
                     /translation="MTKRNWQTLILIAIILVIGIYLVLPGTTRVPLLNNSVRTVLGLD
                     LGGGVQALLEADLPEASVTAENMNTARQIVENRVNGLGVTDAVVQQAGATRILVELPG
                     ETDPEAVLSTLRETGLLEFVDFSSVDPNTAFSLVGTTIATDFAQTAEQASASPGQVYH
                     TVLTGAELEDAGVTVSQTGTYEVTLSFKGDGISKFSEYTTNNVGKILAIVLDKQIISA
                     PSISVPITDGAAVIQGTFTYEEANALAVQLRYGSLPIPLKVVESRTVNPTLGEDSLQK
                     SIRAGMIGFVIVFLFMAIYYRLPGLISIIGIIFYGIIDYAIFRTVPVTLTLPGIAGVL
                     LSTGSALDANILVFERLKEELRAGRNLTQAFALAYQRAWPSIRDSNIATLIT"
     gene            complement(1239..1601)
                     /locus_tag="VN363_08860"
     CDS             complement(1239..1601)
                     /locus_tag="VN363_08860"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84663.1"
                     /translation="MREPQHVIGIVGPCAAGKTTLLNGLRALGLPGKVIAQEHSYVAD
                     MWQRLTNPAILIYLDVSYPVTLERCKLNWTQAEFDIQIERLRHARQHADLQIFSDHLS
                     PEQILAQVVDFLDHQNFV"
     gene            complement(1663..>2338)
                     /locus_tag="VN363_08865"
     CDS             complement(1663..>2338)
                     /locus_tag="VN363_08865"
                     /EC_number="3.1.3.16"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004722 - protein serine/threonine
                     phosphatase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="PP2C family protein-serine/threonine
                     phosphatase"
                     /protein_id="HWQ84664.1"
                     /translation="KYASPNSGDTVEVVERPQGGYSVVLVDGQSSGRGAKTSSVLVVR
                     KVIGLLADGVRDGAAARAASDNLYAERGGKVSATLNIISADLHSRTLLITRNNPNPAY
                     LARGDELRVLNEESRPIGLYRDTRPVITEVDLVAGMTIVAFTDGIVHAGSRTGDRLDV
                     AEVLQDLLDAEEPSAQYLADSLLDHALRLDQGRPVDDISVIVMRVLSRTGDEVRRMSV
                     RLPVEF"
BASE COUNT          549 a          639 c          660 g          490 t
ORIGIN      
        1 caggtgatca gcgtggcgat attcgaatcg cggatggaag gccaggcccg ctggtaggcc
       61 aacgcgaaag cctgggtcag gttgcgcccg gcccgcagct cctctttcag ccgctcaaaa
      121 accaggatat ttgcatccag ggcgctgccg gtgctgagca gtacaccggc gataccggga
      181 agagtcaggg taaccggaac agtccggaaa atggcataat cgataatccc gtaaaaaatg
      241 atcccaatga tcgatatcag gccaggcaag cgatagtaaa ttgccatgaa caggaatacg
      301 attacaaaac cgatcatacc ggcgcggata ctcttttgca gcgaatcctc gccgagggtc
      361 ggattgaccg tgcgggattc gaccactttg agcgggatcg gcagggagcc atagcgcagc
      421 tggacggcca gtgcgttggc ttcttcgtac gtgaaggtac cctggatcac agcggcgcca
      481 tccgtaatcg ggacggagat cgatggggcg gagataatct gcttgtccag aacgattgcc
      541 aggattttcc cgacattatt ggttgtgtat tcgctaaatt ttgagatgcc atcgcctttg
      601 aagctcaggg taacttcgta ggtaccggtc tgactgaccg tcacaccggc atcttccaac
      661 tcggcgccgg taagcacggt gtgataaacc tgacccgggg aggctgaagc ctgttcggcg
      721 gtttgcgcaa aatcagttgc gatcgtcgtc ccgaccaggc tgaaggcagt gttgggatcc
      781 acgctgctga aatcgacgaa ttcgagcaag ccagtttcac gcagcgtgct caagacggct
      841 tcaggatcgg tttcacccgg cagctcgacc aggatgcgag tcgccccagc ctgctggacg
      901 acggcatccg tcacccccag gccgttaacc cggttttcaa cgatctgccg ggctgtgttc
      961 atattttcgg cagtcacaga agcttcaggg agatcagcct ccaggagtgc ctggacccca
     1021 ccacccaggt ccagcccgag tacagtccgg actgaattat tgagcaatgg aacccgggtt
     1081 gtacctggca gaaccagata aattccgata acaagaatga tggcaatcaa gatcaatgtt
     1141 tgccagttgc gtttggtcat acgatgcgag cctccaatat catggaaatg ccagccggag
     1201 gctggaccag aggattatac ccccgttctg attacctgtc agacaaagtt ttgatgatcc
     1261 aggaaatcga cgacctgagc cagaatttgc tcaggtgaga ggtgatcact gaagatctgc
     1321 aggtcggcat gctggcgagc gtgccgcaag cgctcgatct ggatatcaaa ttccgcctgg
     1381 gtccagttga gcttacagcg ctcaagcgtg acagggtatg acacatccag atagatcagt
     1441 atggccggat tggtcagcct ctgccacatg tctgcgacat aggaatgttc ctgggcgatc
     1501 actttaccgg gcaatcccag ggcgcgcaat ccattcagca gcgtggtttt cccggccgca
     1561 cagggaccaa cgattccgat aacatgctga ggttcacgca ttgtctgaaa cttgtaaatg
     1621 gctgctcaca ccggcagcca gtcatttgat taagaaaact gattagaatt cgacaggcag
     1681 gcggacggac attctgcgaa cttcatcgcc ggtgcgagaa agtacgcgca tcacgatcac
     1741 cgagatatca tccacggggc ggccttgatc caggcgcaaa gcatgatcca gtaaagaatc
     1801 tgccagatat tgggcactag gttcttcagc atcaagcaag tcctggagga cctcagcgac
     1861 atccagcctg tcgcctgtcc gcgaaccggc atgcacgatc ccatcggtga aggctacgat
     1921 ggtcataccg gccaccagat ccacctcggt aatcacgggt cgtgtatccc gatacaggcc
     1981 gattggtctg gattcctcgt tcaggacgcg cagctcgtca cctcgcgcaa ggtaagctgg
     2041 attggggtta ttgcgggtga tcagtaatgt gcgcgaatgt aaatcagcgg aaataatatt
     2101 gagggtcgct gagactttcc cgccccgttc ggcatacagg ttgtccgagg ctgcccgtgc
     2161 agcagcccca tcccggacac catctgcgag cagcccaata actttgcgca ccaccaggac
     2221 tgagctggtt ttggcaccgc gtcccgagct ttgaccatcg accaggacga ctgagtaacc
     2281 gccttgcggg cgctcgacga cttcaactgt gtcgccgctg ttgggggacg catatttg
//
LOCUS       DATMXK010000485         3759 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51474, whole genome shotgun sequence.
ACCESSION   DATMXK010000485 DATMXK010000000
VERSION     DATMXK010000485.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3759)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3759)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3759
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51474"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..442)
                     /locus_tag="VN363_08870"
     CDS             complement(<1..442)
                     /locus_tag="VN363_08870"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84665.1"
                     /translation="MRSKLQSPVFSSLLLGGLVILLAVAAFGAGYLFRARQTSTTDFP
                     LLTEAYTIVQNHGLKELPSGPLLEYGMIRGMLEVYADPFSVFVEPVQHELDSNALQGS
                     FGGVGVELSVDAGGYVVLYPFPSSPAVEAGVQDGDRLLQVNDTPI"
     gene            complement(476..2719)
                     /locus_tag="VN363_08875"
     CDS             complement(476..2719)
                     /locus_tag="VN363_08875"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:TIGR02478.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWQ84666.1"
                     /translation="MPPISTIGRIGVLTSGGDAQGMNAALRAIVRTSLDRGIDVYAIY
                     EGYAGMVAGGDLIRKMTWESVGGILQLGGTIIGTARSEEFRTYEGRRQAARNLLEHDI
                     DRLVVIGGDGSLTGANIFRQEWPDLLKELVAAGEITKKSAAAHPALMIAGLVGSIDND
                     MFGTDMTIGADSALHRITEAVDAISSTAASHQRTFVVKVMGRHCGYLALMGGLAGGAD
                     WVLIPESPPDTDDWEKLLIERLKAGRRAGRRDSIIVMAEGAQDRHGKYIGSTHVAEVL
                     EKELGEEVRVTVLGHVQRGGTPSAFDRNLGTRLGYAAVEALLEATPQTEPVLIGIRGN
                     RIVSSPLMECVQQTQGVSAAINSHNYEKAMELRGSSFKESFQTLKTLLRSLPHEPAPG
                     QRRLRLAVMHAGSPAPGMNAAVRAAVRIGLDRGHTMLGVHNGFEGLGRGEVEEMTWMS
                     VNGFAARGGSELGTDRAVPAGKNIYQIARQIEEHAIDGILVIGGFIGYETAYNLYSAR
                     ANFPAFNIPMVLLPASINNNLPGSELSIGADTALNNISDAVDKIKESAVALRRVFVVE
                     VMGRRCGYLALMGGLATGAERVYIHEEGVSLHDLQVDVVKLNQGFTHGKRVGLLIRSE
                     DANQVYSTPFMVALFEEEGADLFDVRQAILGHLQQGGNPSPFDRIQATRMATRCIDYL
                     IDQLEAGTNNSAFIGMIGKEIRFHSLEDFPRMADIPNQRPKEQWWMDIRPIARILAQP
                     SPVKEDA"
     gene            2981..3292
                     /locus_tag="VN363_08880"
     CDS             2981..3292
                     /locus_tag="VN363_08880"
                     /inference="COORDINATES: protein motif:HMM:NF015925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="antibiotic biosynthesis monooxygenase"
                     /protein_id="HWQ84667.1"
                     /translation="MFIVQVFIQVKLEAIEAFKEATLENARSSLQEPGVRRFDLLQET
                     GKSTQFVLWEVYADARANANHKTTAHYARWKETVEEMMAKPRYSLIYHNIFPGDEDWQ
                     P"
     gene            3289..>3759
                     /locus_tag="VN363_08885"
     CDS             3289..>3759
                     /locus_tag="VN363_08885"
                     /EC_number="1.1.1.1"
                     /inference="COORDINATES: protein motif:HMM:NF012678.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="iron-containing alcohol dehydrogenase"
                     /protein_id="HWQ84668.1"
                     /translation="MTAANSFEFTPPQRIVFGEGTAGKTGELVKEWGKRALVVLGASE
                     VVVAPVLISLKAAGLRYETVRVEHEPTLASVEAALEQARKFETEMVLGVGGGSALDTA
                     KAVAALLANPGELLDYLEVVGRGQTLRTASKPCACIPTTAGTGSEATRNAVVGVP"
BASE COUNT          738 a         1092 c         1232 g          697 t
ORIGIN      
        1 gaatgggggt atcgttcacc tgcaggaggc gatcgccgtc ctgcacgcca gcctcgaccg
       61 cagggctcga aggaaatgga tacaggacaa cgtaaccacc ggcgtcgacg ctgagctcaa
      121 ccccaacccc accgaagctg ccctgcaggg cattcgagtc cagctcgtgc tggaccggct
      181 cgacgaagac tgaaaacggg tcagcgtaaa cctccagcat gccgcggatc atgccatact
      241 ccagcagcgg gcccgaggga agctccttca agccgtgatt ctggacgatg gtgtaggctt
      301 cggtgagcag cgggaaatca gtggtggaag tctggcgggc gcggaacagg tatcctgcgc
      361 caaaggctgc cacagccaga aggatgacca gcccgccgag caggaggctg ctgaaaacag
      421 gggattgaag ttttgatctc acgtgatcac caggatcgaa ccggaacgag ccgtctcagg
      481 catcctcttt cactggagaa ggctgggcga ggatacgcgc aattgggcgg atatccatcc
      541 accactgttc cttggggcgc tggttgggaa tatccgccat gcgcgggaag tcttccaggg
      601 aatggaaccg gatctctttg ccgatcatgc cgatgaaggc actgttgttg gtgccggctt
      661 cgagctgatc gatgaggtaa tcgatgcagc gggttgccat gcgggtcgcc tgaatgcgat
      721 cgaagggcga ggggttgccg ccctgctgca ggtgaccgag gatcgcctgg cgcacgtcga
      781 acagatcggc gccttcttcc tcgaacaggg ctaccatgaa gggggtggag taaacctggt
      841 tggcgtcttc gctgcggatc agcaggccca cacgcttgcc atgggtgaag ccctggttga
      901 gcttgaccac atcgacctgc aggtcgtgca gagaaacgcc ttcctcgtgg atgtagaccc
      961 gctcggcgcc ggtcgcgagg ccgcccatca gggccaggta gccgcagcgc cggcccatca
     1021 cttcgacgac aaagacccgc cgcaatgcaa ctgccgattc cttgatctta tccacggcat
     1081 cgctgatatt gttgagagcg gtatcggccc cgatgctaag ttccgaaccg ggcaggttgt
     1141 tgttgatcga ggcgggtaag aggaccatcg ggatattaaa ggccgggaaa ttggcacggg
     1201 cactgtacag gttgtaggcc gtctcgtaac cgataaaacc gccgatcacc aggatgccat
     1261 cgatggcgtg ttcctcaatc tggcgggcga tctggtagat gttcttaccg gcaggtacag
     1321 cccggtcggt gcccagctcc gagccgccgc gcgccgcaaa accatttaca ctcatccagg
     1381 tcatctcttc gacttcgccg cgtcccagac cttcaaagcc gttgtgcaca cccagcatgg
     1441 tatgcccgcg atcgaggccg atccgcaccg cggcgcgcac cgcggcattc atcccgggag
     1501 caggcgagcc ggcatgcatc accgccaggc gcaggcggcg ctgcccggga gcaggctcgt
     1561 ggggcaggga gcgcagcagg gttttgaggg tctggaaaga ttctttgaag ctgctgccgc
     1621 gcagctccat ggctttctcg taattgtggc tgttgatggc cgctgagaca ccctgggttt
     1681 gctggacaca ttccatcagc ggcgagctga cgatgcggtt gccgcggatg ccgatcagca
     1741 ccggctcggt ttgcggggtg gcttcgagca gggcttcgac ggcagcatag cccaggcgcg
     1801 tccccaggtt gcggtcgaag gccgaaggag tgccgccgcg ctggacgtgt ccgaggacgg
     1861 tgacgcgcac ttcctcgccg agttcctttt ccaggacttc ggcgacatgg gtgctgccga
     1921 tgtacttgcc gtgccggtcc tgggcgcctt ccgccatgac gatgatggaa tcgcgccgcc
     1981 cggctctccg cccggctttc aggcgctcga tcagcagctt ctcccagtcg tcggtatcgg
     2041 ggggcgattc ggggatcagc acccagtctg cgccgccagc caggccgccc atcaatgcca
     2101 ggtagccgca gtggcggccc atgaccttga cgacgaaagt gcgctggtgg ctggcagccg
     2161 tgctggagat ggcatccacg gcttcggtga tgcggtgcag ggccgaatcg gcgccgatgg
     2221 tcatatcggt gccgaacata tcgttatcga tcgagccgac cagcccggcg atcatcaggg
     2281 cagggtgggc ggcggcggat ttcttggtga tctcgccggc tgccaccagc tccttgagca
     2341 ggtcgggcca ttcctggcgg aagatattcg ccccggtgag gctgccatcg ccgcctatga
     2401 ccaccaggcg gtcgatatca tgctccagca ggttgcgggc agcctggcgg cggccttcat
     2461 aggtgcggaa ttcttcggag cgggcggtac cgatgatcgt cccgcccagc tggaggatac
     2521 cgccgaccga ttcccaggtc attttgcgga tcagatcgcc gcccgcgacc atgccggcgt
     2581 aaccttcata gatggcgtag acatcgatgc cgcgatccag gctggtgcgg acgatggcgc
     2641 gcagggcggc gttcatgccc tgggcgtcgc cgccgctggt gaggacgccg atgcggccga
     2701 tggtggagat gggtggcata cgtttgtgct cctgaaaccg ccaactggcg gactgaatat
     2761 tcgtcaaata ttaccaatca tacagggaaa gctataaaaa tggtagagga tatcgaatgc
     2821 caggttgatc tctccaccgc tgaagtaaac ctgatttgct agaccgtgat gatgggtcag
     2881 catggcgatc ctttggatac cggctccaaa ataaggattt cctcccctgg actttgtggc
     2941 atactatgga ttaaatccaa ccgctgcctg gaggccaatc atgtttatcg tacaggtttt
     3001 catacaggtc aagctcgaag cgattgaggc gttcaaggaa gccacgctgg agaacgcccg
     3061 cagcagcctg caggagccag gcgtacggcg cttcgatctg ctgcaggaaa ccggcaagtc
     3121 cacccaattt gtgctgtggg aggtgtatgc cgatgccagg gctaacgcca accacaagac
     3181 gactgcccat tatgctcgct ggaaggagac ggtggaagag atgatggcaa agccgcgcta
     3241 cagcctgatc taccacaata tcttcccggg cgatgaggac tggcagccat gaccgcagcc
     3301 aacagctttg aatttacccc gccacagcgg atcgtctttg gggaaggcac tgccgggaag
     3361 actggcgagc tggtaaagga atgggggaag cgggcgctgg tggtactggg cgccagcgaa
     3421 gtggtggtcg ccccggtgct gatcagcctg aaggctgccg gattaagata tgagacggtg
     3481 cgggtggaac acgagccgac cctggcgagc gtggaagctg ccctggagca ggcgcgcaag
     3541 tttgagaccg agatggtgct gggagtgggt ggtggcagcg ccctggacac cgcgaaagcc
     3601 gtggcagccc tgctggcgaa ccccggcgag ctgctggatt acctggaggt ggttgggcgc
     3661 ggtcagactt tgcggacagc cagcaaaccc tgcgcctgca tccccacgac cgccggaacc
     3721 ggctcagaag cgacccgcaa cgcggtggtg ggcgtgccc
//
LOCUS       DATMXK010000486         3145 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_112072, whole genome shotgun sequence.
ACCESSION   DATMXK010000486 DATMXK010000000
VERSION     DATMXK010000486.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3145)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3145)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3145
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_112072"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..498
                     /locus_tag="VN363_08890"
     CDS             <1..498
                     /locus_tag="VN363_08890"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ84669.1"
                     /translation="GGYLAQFLVAWHPERVTRAVFANTFAPTTSYKEKYGIAGSLIPY
                     VPEWLVMDILKVSFKTGIYPTSGNDEFTLAFLNEIASGRMSKAQVSGRYQCVVELFNL
                     PAETNVPVLIIEASNDPLVDADLRAQLRAAYPAAQVVTVDIGHFPYQTTPEFYNQTLL
                     TFFSQ"
     gene            594..1460
                     /locus_tag="VN363_08895"
     CDS             594..1460
                     /locus_tag="VN363_08895"
                     /inference="COORDINATES: protein motif:HMM:NF017536.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pirin family protein"
                     /protein_id="HWQ84670.1"
                     /translation="MTYTFRNVKQIIEPQTVIEGADVRLRRSFGPNRSNLFDPFLLFD
                     HFAFNDPVEGPIQGFPTHPHRGIETVTYMLRGSVRHRDSLGNTGIIGPGDVQWMTSGR
                     GIMHEELPRRDPDGIIQGFQLWVNLPAAQKMSQPRYQEVSATTIPCVETPTTLVRVVA
                     GEYNGVNGPVTDIAAQPIYMDVTLQPGAEFSLPTPTQDFAIAYVFEGEGVFGGGESEP
                     GDFIQAVRMLVFDEGDKIHVESGPAAAVRFMLMVGEPFQEPIFPYGPFVMNTQAEIQQ
                     ALADLRSGTFVQ"
     gene            complement(1523..2758)
                     /locus_tag="VN363_08900"
     CDS             complement(1523..2758)
                     /locus_tag="VN363_08900"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF013704.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0008170 - N-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0009307 - DNA restriction-modification
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="site-specific DNA-methyltransferase"
                     /protein_id="HWQ84671.1"
                     /translation="MPILNWDTKTPPAQLPAQLDTRAVIYPCGVGYPDSPAADTLVQG
                     DNLAVMSALLEAYAGKIDLIYADPPFFTNRAFPARIGQGEDSRRPQDWKLGVGYPDRW
                     VRLEDYLDMLYPRLALMHRLLAPTGTLYLHLDWHADAYARLLLDEIFGAERLLNEIIW
                     VYHGPSPIRSAFNRKHDTILAYTKSSDYNFNADAVREPYNPNTVKTFNSSRKAGFGKV
                     PNLARGKVPEDWWYFPVVARLHAERTGYPTQKPEALLERIILASSKPGDTVADFFCGS
                     GTTPVVASRLGRSYLASDLTPQAIHTTQARLIERGAPAFQLKQIAGSAPPTPENNSTS
                     PVRRAGNQLILDESCLEELVFWDIDPAWDGHVYRSAAQSIRTRRQPPASGIELPGLGE
                     QVCARIIRTSGKEQYYLVS"
     gene            complement(2817..>3145)
                     /locus_tag="VN363_08905"
     CDS             complement(2817..>3145)
                     /locus_tag="VN363_08905"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="GTPase"
                     /protein_id="HWQ84672.1"
                     /translation="GAGWVAARRFGAAEIVDPRPFAVGSIRTTYEKYPLTGAILPAMG
                     YGEAQMHDLEATIRAADVDLVIIGTPIDLTRVIKIDKPSQRVRYELQEIGKPTLEDIL
                     KAKFGK"
BASE COUNT          632 a          904 c          951 g          658 t
ORIGIN      
        1 ggcggctacc tggcgcagtt tctggttgcc tggcatcccg agcgggtcac cagggcagtc
       61 ttcgcgaata cattcgcacc aaccacgagc tataaagaaa aatacggcat tgcgggcagc
      121 ctgattcctt acgttccaga atggctggtg atggatatct tgaaagtcag ttttaagacc
      181 ggtatttatc cgacctccgg taacgatgaa tttacgctgg cgtttctaaa tgaaatagca
      241 tccggaagga tgagcaaggc ccaggtatcc gggcgctacc agtgcgtggt ggagttattc
      301 aacttacctg cggaaacgaa cgtcccggtg ttgatcatcg aagccagcaa cgatccgctg
      361 gtcgacgccg accttcgtgc ccaactgcgt gccgcctatc ctgccgccca ggtggtgacc
      421 gtcgacattg gtcattttcc atatcagacc acgcccgaat tctacaacca gacgctgctt
      481 acattcttca gccagtgaga actccggagg gtttcaaaac caaggaagcc tgccatatgc
      541 agttataata ggatcgttga agtctgatta ccggttacca gaaggaatct ccaatgacct
      601 atacatttcg gaatgtaaaa caaataatcg aacctcaaac cgtaattgaa ggcgccgatg
      661 tgcgcttgcg ccgctctttt gggcctaacc gatccaacct gttcgatcct ttcctgctgt
      721 tcgatcattt tgctttcaac gatccagtcg aagggccgat ccagggtttt cccactcacc
      781 cccatcgcgg catcgaaacc gtgacctata tgctgcgagg cagtgtccgc caccgggata
      841 gtctgggtaa tacggggatc atcggacccg gcgacgtgca gtggatgacc agcgggcggg
      901 gtatcatgca tgaggaatta ccccgccgtg atccggatgg catcattcag ggcttccagc
      961 tgtgggtgaa tttacccgcc gctcaaaaga tgagccagcc ccgctaccag gaagtcagcg
     1021 ccaccaccat accgtgcgtg gagactccca cgactctggt gcgggttgtc gctggcgagt
     1081 acaacggggt taacggcccg gtcacggaca tcgctgccca accgatctac atggatgtca
     1141 ctctccagcc gggggcggag ttcagcctgc ccacccccac ccaggatttc gccattgcct
     1201 atgtgttcga aggggaaggt gtgtttggcg gcggcgagag cgagccgggt gactttatcc
     1261 aggcagtcag gatgctggtc ttcgatgaag gggataagat ccacgtcgag agcggtcctg
     1321 cggctgcagt gcgctttatg ctcatggtcg gcgagccatt ccaggagccg atcttcccct
     1381 acggcccatt cgtaatgaac acccaggctg agatccagca ggccctggca gatttgcgca
     1441 gcggcacctt cgtgcagtag tctcaaagca cagggcgcgc ctggcttaaa aagctggcgc
     1501 gccctgtgct ttcccctcta tcctacgaaa ccagataata ctgctctttg ccgctcgtcc
     1561 ggatgatacg cgcacagacc tgctctccca ggccgggtaa ttcgattccg ctggcaggcg
     1621 gctggcggcg ggtgcgtatg gattgggcgg cgctgcgata cacgtgacca tcccatgcgg
     1681 gatcgatatc ccagaaaacg agttcttcca ggcaagattc atccaggatg agctggtttc
     1741 cggcgcgccg cacaggcgaa gtagaattgt tttcgggtgt gggcggagct gacccggcaa
     1801 tttgcttcag ctggaaggct ggtgcacccc gctcgatcag ccgagcctgg gtggtgtgga
     1861 tcgcctgcgg ggtcaggtcg ctggcgagat agctccggcc gagccggctg gcgaccacag
     1921 gtgtggtacc tgaaccgcag aagaaatccg ccacggtatc ccccggtttc gaagatgcca
     1981 ggatgatacg ctccagcaga gcttccggtt tctgggtggg ataaccggtg cgctcagcat
     2041 gcaggcgcgc caccaccggg aaataccacc agtcctcggg taccttaccc cgcgccaggt
     2101 tgggtacctt accaaacccg gcttttctgg atgaattaaa cgtcttgacg gtatttgggt
     2161 tgtacggctc gcgcaccgcg tcggcattaa agttgtagtc gctgcttttg gtgtaagcca
     2221 ggatggtatc gtgcttgcgg ttgaacgcgc tgcggatggg cgaaggtccg tggtagaccc
     2281 agataatctc attgagcaag cgctcggcgc cgaagatctc atccaggagc aggcgggcgt
     2341 aggcgtcggc gtgccagtcc aggtgcaggt aaagtgtgcc ggttggcgcc agcaggcggt
     2401 gcatcagcgc caggcgcggg tagagcatat ccaggtaatc ttccaatcgc acccaacggt
     2461 cggggtaacc tacccccagc ttccaatcct gcgggcggcg agaatcttcg ccctgcccga
     2521 tgcgcgccgg gaaagcccgg ttggtgaaaa atggggggtc ggcgtagatc agatcgatct
     2581 tgcccgcata cgcctcgagc agggccgaca tcaccgccag gttgtcgccc tggaccaggg
     2641 tatcggcagc tggagagtcc gggtagccta cgccgcaagg ataaatcaca gcgcgggtat
     2701 ccaattgggc gggtagctga gccggtgggg ttttggtatc ccagttcagg ataggcatca
     2761 ggtgggggag ctcattgggg caggcgcatc tcgctgcccg ccccggctgg ttcagtctat
     2821 ttgccgaatt tagctttcag gatgtcttcc agggtcggct tgccgatctc ctgcagctcg
     2881 tagcgcaccc gctggctggg cttatcgatc ttgatcaccc gggtcagatc aatcggtgtg
     2941 ccgatgatga ccaaatccac atccgcggcg cggatggtgg cttccaggtc gtgcatctgg
     3001 gcttcgccat accccatcgc cggcaggatg gcgccggtga gggggtattt ctcataggta
     3061 gtgcggatcg agcccaccgc gaaggggcgg ggatccacga tctcggccgc gccaaagcgc
     3121 cgggctgcca cccagccggc cccgt
//
LOCUS       DATMXK010000487         3443 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_40425, whole genome shotgun sequence.
ACCESSION   DATMXK010000487 DATMXK010000000
VERSION     DATMXK010000487.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3443)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3443)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3443
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40425"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..239)
                     /locus_tag="VN363_08910"
     CDS             complement(<1..239)
                     /locus_tag="VN363_08910"
                     /inference="COORDINATES: protein motif:HMM:NF022734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3090 family protein"
                     /protein_id="HWQ84673.1"
                     /translation="MAPFDIDLKPVTHITTDAIGQPGKRVFYIQGWQDERTVTLIVEK
                     VQIQSLAVGLEQFLVEVSEKYADLTEASADYDQEK"
     gene            complement(358..975)
                     /locus_tag="VN363_08915"
     CDS             complement(358..975)
                     /locus_tag="VN363_08915"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWQ84674.1"
                     /translation="MPAIFLIRHGENDYVKKHRLAGRMPGVHLNKKGQEQVQLLADKL
                     KEVPIKAVYSSPLDRTMETAAPLAQALGLEVIPRPGLIETDFGEWQGESLKKLNRLKI
                     WRVVQNRPSLMRFPGGESFAELQMRYVHELEYICSQHEPKDIVACVSHADPIRLAVAY
                     YIGLSLDMFQRLMVLPGSISVLWIADGASQLLALNVDPAFSLFKP"
     gene            complement(982..2391)
                     /locus_tag="VN363_08920"
     CDS             complement(982..2391)
                     /locus_tag="VN363_08920"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558522.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ84675.1"
                     /translation="MDLFTHAMNDRMKTEAPLAARMRPRTLDEFIGQEHIVGVGKLLR
                     RAIEADRLFSSILLWGPPGTGKTTLAMVIANLTKSHFETLSAVLAGKAELREVIEHAL
                     ERRKLYGKRTILFVDEVHRWNRAQQDALLPHVENGTVILIGATTENPYFEVIGALVSR
                     SRVFQMRPLDDEAVRQILLNALHDPERGYGTRPVEIDEDALQHLINVAGGDARNALNA
                     LELAVESTGAGVGQSIHVSLEVAQESIQRRAVLYDKDGDAHYDTISAFIKSVRGSDPD
                     AALYWLAKMLYAGEDPRFILRRLLILAGEDIGLGDPLGLVVASAAAQAFDYVGLPEGI
                     YPLVEATLYLATAPKSNSAGAYFKAFQQIEAEGVGDVPDPLKDKNRDARALGHGEGYL
                     YPHTDPEHFLPQQYLPGPLLGTYFYAPSEQGYEAQVKARLEHWRAAQAAALGITQTET
                     LPELTEEAIKDIKGKHKAT"
     gene            complement(2543..2986)
                     /locus_tag="VN363_08925"
     CDS             complement(2543..2986)
                     /locus_tag="VN363_08925"
                     /inference="COORDINATES: protein motif:HMM:NF025734.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="heme-binding domain-containing protein"
                     /protein_id="HWQ84676.1"
                     /translation="MKKVFLIVLGLIVLIQLLPYGRSHNNPPVVQEPAWQGVETRPLA
                     QRACFDCHSNETEWAWYSNIAPASWLIQHDVEEGRSKLNFSEWGRGEQETGEIGEVIL
                     NGEMPPAQYTLIHPDSRLSTTENEMLLNGFSAMGIAGAEEREKGD"
     gene            3185..>3443
                     /locus_tag="VN363_08930"
     CDS             3185..>3443
                     /locus_tag="VN363_08930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84677.1"
                     /translation="MVGDNKRVAIWLARTAVGLVFAVNVYCALVFIFQPQNYAGGFEL
                     SGTSGEIIVQSFGILFLMWNATYPPVLLHPHTQRTLFGVILV"
BASE COUNT          742 a          985 c          890 g          826 t
ORIGIN      
        1 atcttctcct gatcataatc cgcgctggct tcggtcaggt cagcatattt ttcgcttact
       61 tccacaagaa attgctccaa acccaccgcc aaagattgga tctggacctt ttccacgatt
      121 aatgtgactg tacgctcgtc ctgccatccc tggatataaa acactcgttt accgggttga
      181 ccaattgcat cggtcgtgat gtgggtgacc ggtttcagat cgatatcgaa tggcgccatc
      241 ctcgcctccc cggctcaaat tcgagccgct accaattcag tgtgtgatgc tggcagtttt
      301 ggcccgccat taaaaattct tgcatttgaa atggcagtcg cgcctagtat accagagtca
      361 gggcttgaac aagctaaatg caggatcaac attaagagcc aggagctggc tggcgccgtc
      421 ggcaatccag agcacactga tcgaaccggg cagcaccatc aagcgttgga acatatccaa
      481 cgataggcca atataatatg ccactgccag gcggattgga tcggcgtgcg atacgcaggc
      541 tacgatatcc ttgggctcgt gctggctgca gatgtattcc agctcatgta catagcgcat
      601 ctggagctcg gcaaagcttt cgccgcccgg aaagcgcatc aatgaaggcc tgttttgtac
      661 cacgcgccag atcttcaagc ggttcagctt tttcaggctc tcgccctgcc attccccaaa
      721 atcggtttcg atcaaacccg ggcgcgggat cacctccagc cccaaagctt gagccagcgg
      781 cgcggcggtt tccatggtac ggtccagggg gctgctgtat acggctttta tcggaacttc
      841 tttcagctta tccgccagca gctggacctg ctcctggcct tttttattca aatgtacgcc
      901 tggcattcgg cctgccaggc ggtgtttctt gacataatcg ttttcaccgt gtcgaattaa
      961 gaagattgcc ggcatagttc cctaagtagc cttatgcttg cccttgatat ctttgatcgc
     1021 ctcttctgta agctccggca gagtctcggt ctgggtaatt cccagggctg cagcctgagc
     1081 cgcgcgccag tgttccaggc gtgctttgac ctgagcttcg taaccctgtt cagatggagc
     1141 atagaagtag gtaccgagca agggtccagg caggtactgc tgggggagga aatgctccgg
     1201 gtcggtatgc gggtaaaggt aaccttcgcc atgccccagg gccctggcat cccgattttt
     1261 atctttgagc ggatcgggta catcccccac accttctgcc tcgatctgtt ggaatgcttt
     1321 gaaatacgcc ccggcagagt tcgatttggg agcagttgcc agataaaggg tcgcctcaac
     1381 cagcggatag attccctccg gcagccccac gtagtcgaat gcctgggcgg cagcactggc
     1441 aacgaccaat ccgagtggat cgcccagccc gatatcctcg cctgccagga tcaacagccg
     1501 gcgcaggatg aaacgtgggt cctccccggc atacagcatc ttcgccagcc agtacagggc
     1561 ggcatctgga tcggagcccc tcacggattt gataaaggca gaaatcgtat cgtaatgggc
     1621 gtcgccatcc ttatcgtaca gcaccgcccg gcgctggatc gactcctgag caacttcaag
     1681 ggacacatga atcgactgtc ccaccccggc gccggtgctc tccactgcca gctcgagggc
     1741 gttcagggcg ttacgggcgt ccccgccggc tacgttgatc aagtgctgta aggcatcttc
     1801 gtcgatctca accgggcgcg ttccgtaccc tcgctcaggg tcgtgcaggg cgttaagcaa
     1861 gatctggcgc accgcctcat catccagagg tcgcatctga aaaacccgtg agcgcgagac
     1921 caacgctccg attacttcaa aataaggatt ttcggtggtg gccccgatca aaattactgt
     1981 gccgttctca acatgcggca gcagcgcatc ctgctgagcg cggttccagc gatggacctc
     2041 gtccacaaac aggatcgtac gtttgccgta cagcttgcgc cgttccaggg cgtgctctat
     2101 cacctcgcgc agctccgctt tgcccgccag gacagccgaa agggtctcga aatggctctt
     2161 ggtcaggttg gcaatcacca ttgccagggt ggttttaccc gtaccgggcg gaccccacaa
     2221 caggatcgac gaaaacagcc tgtctgcctc aatcgcccgg cggagcagct tccccactcc
     2281 cacgatatgt tcctgtccga taaattcatc cagggtgcgc gggcgcatgc gcgccgccag
     2341 aggcgcttcg gttttcatgc gatcgttcat ggcgtgggtg aagaggtcca tggcgacaat
     2401 tatactctga ggaacctgac gacccttgcc ggactgatcg gactcttaaa tacgtgaaaa
     2461 cgctttttgt cccgtattgc acatttttcc ttttgactac tcgaacccat tcaatttcga
     2521 ataagatatt aatgtgcgcg gcttagtcgc ctttttctct ttcctcagct cctgcaattc
     2581 ccatcgctga aaagccgttc agcagcatct cgttctccgt ggtcgacagg cgagaatcag
     2641 ggtggatcag ggtatattga gccggaggca tctccccgtt caggatcact tcgccaattt
     2701 ctcctgtctc ctgttcgccg cgaccccact ccgaaaagtt aagcttactg cggccctctt
     2761 ctacatcgtg ttggatgagc catgaagccg gagcgatgtt gctgtaccag gcccactcgg
     2821 tttcattgct gtgacaatcg aagcaagccc gttgagccag gggtcgcgtt tcaacaccct
     2881 gccaggcagg ctcctggacc accgggggat tgttatggct acgcccatat ggaaggagct
     2941 ggatcaacac aatcagcccg agtacgatca ggaaaacctt cttcattgaa gcctcctcag
     3001 gaaaaattta gccggttcga tggatatatt gaatcatacc accagtacat ctggggcgga
     3061 gcccaatgtt atcctggcag ctacgctcaa acctatctca acaaagtgca gatcagctgt
     3121 gaaaaatgta taatctattc atgcaaggtt gaaatacagg aagtggacat tattggaagt
     3181 ataaatggtc ggtgataaca aacgtgtagc aatctggctg gcgcgcactg cagttgggct
     3241 tgtatttgca gtgaatgttt actgcgcgct ggtattcatc ttccagcctc aaaattatgc
     3301 cggcggcttt gagcttagtg gtacttcagg cgagattatc gtccagagtt ttgggattct
     3361 gttcctgatg tggaacgcca cctacccgcc cgttttgctc cacccacaca ctcagcgcac
     3421 tttattcggc gttatcctgg tcc
//
LOCUS       DATMXK010000488         2031 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_158477, whole genome shotgun sequence.
ACCESSION   DATMXK010000488 DATMXK010000000
VERSION     DATMXK010000488.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2031)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2031)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2031
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_158477"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2031
                     /locus_tag="VN363_08935"
     CDS             <1..>2031
                     /locus_tag="VN363_08935"
                     /inference="COORDINATES: protein motif:HMM:NF024777.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="LamG-like jellyroll fold domain-containing
                     protein"
                     /protein_id="HWQ84678.1"
                     /translation="LYGDYYGGGQGSGIIPVTGTYLLRVEPYYNYYGEYQFRLTLAPQ
                     GTQVESEGNDSTGSADPLSLPLNNGSRSATVLGYIAYNDSSDFFRLANLSAGATIDLN
                     IAYPTTSGLLGSLFVYDANGSIVAHGPAGAPSLTYTVPPGGDGVHYVRAWDAGPVGAL
                     KFGHDGYALRLTGSEWINFTNAVIPASGDFTVECWAVSDAPGTLREILSQGSGGNAFY
                     LGSENGRIRAGDGWSDTGIAFPVSGWHHFAVVKSSTNTLLFVDGILQAVKGASIPNPV
                     ASTPLRIGRQYGSYGEYWRGSIDEVRIWNVARTPAAILNTMSTRLGGSEAGLVGYWHF
                     DQGPATTSGPDFVSDATAFANTGAGQGNLTWTSTARTNARPSSLFSEYLLHLELQDTA
                     SAAITSVTLPPAGSTNMSIWDRFNLGLSKDIDPSLNNRNRALYLYGGHAYTITSDAVT
                     WYNAEVQARALGGHLVTINDALENDWLLSTFSSHLNFWTGINDEAQKGVYVWASAEPA
                     GYTNWASGQPNNSNNQDYGTMRAGGQWFTDSPSTTHRGIIEVAGPDMDADGLPDSIDP
                     YPNDPFNAFDLRASGPDGLFDTPDDGLYHVSHANYSSGLSLSFTVSDGPLQPGNYRFM
                     VTGSLRDLFGNPMTVPFTHYFTIDDLPGYALENRNNGSTTSATPLPMVEDPPG"
BASE COUNT          395 a          659 c          555 g          422 t
ORIGIN      
        1 tctgtacggt gattactacg gcggagggca gggcagtggc atcattccgg tcaccggcac
       61 ctacctgctg agggtggaac cctattacaa ttattatggt gagtaccaat tccgcctcac
      121 cttggcgccg cagggcaccc aggtggagtc ggaaggcaac gacagcaccg gcagcgccga
      181 tcctctcagc ctgccgctca acaacggatc ccggtccgca accgttctgg gctacatcgc
      241 ctacaacgac agtagcgact tcttccgtct ggccaatctt tcggccggtg ccacaattga
      301 cctgaacatc gcctatccga ctacaagcgg gctgctcgga agtctgtttg tgtatgacgc
      361 caacggctcg attgtggccc atggcccggc tggagccccg tcacttacct ataccgtgcc
      421 cccaggtgga gacggggttc actatgttcg tgcctgggat gccggcccgg ttggcgccct
      481 gaaattcggc cacgacggct acgccttgcg cctgactggc agcgagtgga tcaacttcac
      541 taacgcggtc atccccgctt cgggcgactt cacggtcgaa tgctgggccg tcagtgatgc
      601 acccggcaca ctccgcgaaa ttctctccca gggctcgggc ggaaacgcct tctatctggg
      661 ttccgaaaac ggacgaatcc gcgcgggtga cggatggtcc gacaccggca tcgctttccc
      721 tgtatccggc tggcaccatt ttgccgtcgt taaaagctcc accaacacgc ttttgttcgt
      781 ggacgggatc ctgcaggcgg tcaaaggggc atccatcccg aatccggtgg cttccactcc
      841 cctgcgcatc ggccgccagt atggcagcta cggggagtac tggcgcggtt cgatcgatga
      901 agtcagaatc tggaacgtgg cccgtacacc ggctgcaatt ctcaatacga tgtccacccg
      961 gcttggcggc agcgaagccg gcctggtagg gtactggcat ttcgaccagg gccccgctac
     1021 gaccagcggg cctgattttg tcagcgatgc cactgccttt gccaacaccg gcgccggtca
     1081 gggtaatctc acttggacat ccactgctcg caccaatgct cggccttcaa gcttgttctc
     1141 ggaatacctg ttgcacctgg aattgcagga caccgcttcg gcagccatca cctccgtgac
     1201 tcttccgcct gccggctcca ctaacatgag catctgggac cgcttcaatc ttggtctgag
     1261 caaggatatc gatccgtcgc tcaacaacag aaatcgtgcc ctgtacctct acggcggtca
     1321 tgcctacacc atcacctccg acgctgtgac ctggtacaat gccgaggttc aggctcgcgc
     1381 cctgggcggc cacctggtca ccatcaatga cgcgttggag aacgattggc tgctttcgac
     1441 cttctcctct cacctcaatt tttggaccgg tatcaatgac gaggcccaaa aaggagtgta
     1501 cgtttgggct agcgccgagc cggcaggcta tacgaactgg gccagcggcc agcccaataa
     1561 cagcaacaat caggattacg gcacgatgcg cgcgggcggc cagtggttca ccgacagtcc
     1621 cagtacgacc caccgcggca tcatcgaagt ggccgggccc gatatggatg cggacggcct
     1681 tcccgattcg atcgatccgt atcccaatga tccgttcaac gcgttcgatc tgcgggcatc
     1741 agggcctgac ggccttttcg acactccgga cgacggcctg tatcatgtca gccacgccaa
     1801 ctacagttcc gggttatccc tgagttttac tgtcagtgac ggcccactgc aaccgggcaa
     1861 ttaccggttc atggtcaccg gctccttgcg ggacctgttc ggcaacccca tgacagtgcc
     1921 ctttacccat tacttcacga tcgacgatct tccgggttat gccttggaga atcgcaataa
     1981 cggatcaacc acctcggcca cgccgctccc catggttgaa gatccgcccg g
//
LOCUS       DATMXK010000489         2765 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51490, whole genome shotgun sequence.
ACCESSION   DATMXK010000489 DATMXK010000000
VERSION     DATMXK010000489.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2765)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2765)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2765
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51490"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..315
                     /locus_tag="VN363_08940"
     CDS             <1..315
                     /locus_tag="VN363_08940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84679.1"
                     /translation="DIKNLHQATRSVQELSQKYLEKSNKVSHFLQGIGLVRMVPLPFL
                     KLPQAQAVIAGLVTALLGYLLYTGYDHVDSGRITFFNKFGVNIPDRVSGVHKTIETAL
                     KV"
     gene            378..>2765
                     /locus_tag="VN363_08945"
     CDS             378..>2765
                     /locus_tag="VN363_08945"
                     /inference="COORDINATES: protein motif:HMM:NF044287.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="zinc-ribbon domain-containing protein"
                     /protein_id="HWQ84680.1"
                     /translation="MNCPKCSQPIPDHARYCPSCGTGVDVAARVDVRIDVEHNQGRVV
                     GVQTDAIHGDVYGNSVYQVQVYVLSESSRAQPQGSPPALPSGPYKFLSPYTPLDRAIF
                     KGRQVEIEQAVRRLGEGRLLVLYGPTGVGKTSLLAAGVIPELIGAGALAVHIRDYSRP
                     LGEALRAALQGSQAQLALSLPDGDDLPGLLTILHSQLNGTLVLVLDQFESLFHSQMDP
                     QLYAQIINELVSSLRQLEPEYLRLILSIEDNALVRLSDLQQALPDVFRSFMQLDPLGP
                     DQARAAILEPLEVFKPPPVYLSAGLVDSLIIPDLIELSPDMPAEVYPPHLQIVCHRLF
                     ELAVKLSPPEIDQNLYVNQSGGAEGILAGFLDETLRLGLGSGVEECNRCLIALATAGD
                     LAWVAAEELAIDGLDKNQIQAGLERLEQAGLLVSRLNQQRVEFAFTSPIVAQAVRRMA
                     GPEIENLGQARADLERGWSGWLAHGDIIPPRPLRRISAVAQQLMPNPLQGLLLIRSAL
                     SDGSSPAVWLPGLATGEGLALVTALEGVDSNPDPHPSVTRLAQAKSILGIDPDQPEVA
                     GDGGFGLLAKTAVGSRDPAVRGAAILALQAPDAHQALSRLDWALAALSGSWQRFWRKS
                     ELRGLLEDEVQIFYSLDRKLPLPQRSGIWLWRFLRSLRRERTTILWSALGAALGAGVG
                     LGLWRGLVAWLAGSTVPGFLGFVHVIYGILLGGSLSLGINLGRMIRLDRITPLKKAPD
                     NRKIDWLPVLLGGIGFGLAHAWIFTLNGGSLLSNSSLVLGSGFLAGLLLSALLVKP"
BASE COUNT          545 a          726 c          808 g          686 t
ORIGIN      
        1 gatatcaaaa accttcacca ggcgacgcgt tctgtccagg aattgagcca gaagtacctg
       61 gaaaagtcca ataaggtcag ccatttctta caaggaatag gactggtgcg gatggtgcca
      121 ctgccattct tgaagctccc ccaggcgcag gcagtgattg ccgggctggt gacagctttg
      181 ctgggatatt tactttacac cggctatgac cacgttgatt ccgggcggat cacgtttttt
      241 aataaattcg gggtgaatat tccggatcgc gtttcaggtg tgcataagac aattgagacg
      301 gcgttgaaag tttaaatacc gattatcttt cagaccctgt ttgggttatt agacagggaa
      361 tgattgggca ggcttttatg aattgcccga aatgctcaca accgatacca gatcatgccc
      421 gctactgccc gtcgtgtggg acaggtgtcg atgtagcggc gcgagtcgat gtacgtatcg
      481 atgtcgagca taaccagggt cgtgtcgtcg gcgtccagac ggatgccatt cacggcgatg
      541 tctatggaaa tagtgtctat caggtgcagg tctatgtgct gagcgagtcg agccgggctc
      601 agccccaagg cagtccgcca gccctgccct ccggaccgta taaatttctt tccccttata
      661 ccccacttga tcgagctatc tttaaaggcc ggcaggttga aatcgagcag gctgtccgcc
      721 gccttggcga aggccgtttg ctggtattgt atggtccaac aggtgtcggt aagacttccc
      781 tcctagccgc cggagtaatc cccgagctga tcggagccgg tgcactggct gtccatatcc
      841 gcgattactc acgtccactt ggagaggcct tacgcgctgc cctgcaaggc agtcaggctc
      901 agctggctct ctcacttccg gatggggatg atttacccgg gctgctgaca attctacaca
      961 gccagttgaa tggtacttta gtgctggttc tggaccaatt cgagagtctg tttcacagcc
     1021 agatggatcc tcagctttat gctcaaatta ttaacgagct ggtgagcagt ctgcgtcaat
     1081 tggagccaga atatctccgc ttgatcctat caattgagga taatgccctg gtgcgcctga
     1141 gcgatctgca gcaagcgctt ccggatgtgt ttcggtcctt tatgcagctg gatcccctgg
     1201 gtcctgatca ggcccgggca gcaatcctgg agcctctcga ggttttcaaa cccccaccag
     1261 tttatctttc agcgggcctg gtagattcac tgattatccc cgatctgatt gagctcagcc
     1321 ccgatatgcc tgcagaagta tacccccctc atctccagat cgtctgccac cgtctcttcg
     1381 aattggcggt taagctctcg ccgcctgaga tcgaccagaa tctgtatgtg aatcaatccg
     1441 gtggggcgga agggatcctg gcaggctttc tggatgagac cctgcgcctg ggtttggggt
     1501 caggcgtgga ggagtgtaac cgttgtctga ttgcactcgc tactgctgga gatttagcct
     1561 gggtagccgc ggaggaattg gcgattgatg gtttggataa aaatcaaatt caggctggat
     1621 tggaacgcct ggagcaagcc ggcttgttgg tcagccgttt gaaccagcag cgggttgaat
     1681 ttgcttttac aagtcctatc gttgcccagg cagtccggag aatggctgga cctgagattg
     1741 aaaatctcgg acaggctcgg gcagatctcg agcgcggctg gtctggctgg ctggcccatg
     1801 gcgatatcat tccgcctcgc ccactgcgca ggatttcggc agtcgcacaa cagctaatgc
     1861 cgaatccact gcaaggcctg ctgttgatcc gttcagccct atctgacgga tcttcgccag
     1921 cggtatggct gcctggcctg gcaactgggg aaggtcttgc cctcgtcacc gcgctggaag
     1981 gcgtcgattc aaatccagat ccgcacccga gcgtgacccg tctggctcag gcgaaaagca
     2041 tcctggggat agaccccgat cagccagagg tcgctggaga tggcggcttt ggtttattgg
     2101 caaaaactgc tgttggcagc cgcgacccgg ctgtccgcgg cgcggcgatc ctggctttac
     2161 aggctcctga cgcccatcaa gccctcagcc gcctggattg ggctctggct gccctctcag
     2221 gcagctggca gcgtttctgg cggaaatccg agctgcgcgg tttgcttgaa gacgaggtgc
     2281 agatattcta cagcctggat cgtaaactac ctctgccgca gcgctccgga atctggttat
     2341 ggcgtttcct gcgctcatta cgtagagagc ggacgaccat cctgtggagc gcacttggag
     2401 cggctttggg ggcaggcgta gggctgggtc tctggcgtgg tctggttgcc tggctggcgg
     2461 gctcaaccgt tccaggcttt ttaggctttg tccatgtgat ttatgggata ctgctgggag
     2521 gtagtctgag cctggggatc aacctgggtc ggatgatccg tctggatcga attaccccct
     2581 tgaaaaaagc acctgataat cgcaagattg actggctgcc ggtgctttta gggggaatcg
     2641 ggtttggtct ggcccatgcc tggatattta cattgaatgg gggcagcctg ctgagcaaca
     2701 gctcccttgt tttggggtct ggtttcctgg caggtctgct actcagcgct ttgctggtga
     2761 aacca
//
LOCUS       DATMXK010000490         3259 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_129757, whole genome shotgun sequence.
ACCESSION   DATMXK010000490 DATMXK010000000
VERSION     DATMXK010000490.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3259)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3259)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3259
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_129757"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1397)
                     /gene="mutL"
                     /locus_tag="VN363_08950"
     CDS             complement(<1..1397)
                     /gene="mutL"
                     /locus_tag="VN363_08950"
                     /inference="COORDINATES: protein motif:HMM:TIGR00585.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006298 - mismatch repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA mismatch repair endonuclease MutL"
                     /protein_id="HWQ84681.1"
                     /translation="MPIRILQPEVASQIAAGEVVERPASVIKELLENSLDAGSTFIQI
                     HVDEAGKRLIEVADDGAGIPESELALAVERHATSKLSSASELSRIQSLGFRGEALASI
                     ASVSRFTITSRTDQSANGTRLRGEGGQMNPVETVGAPVGTVVRVENLFHNVPARLKFL
                     KHDTTERRQIDTLVTRYALAYPQVRFSLQQDGRPVLQTSGNGNRREILATLYGVDVAR
                     EMLEVISEDPEITVTGFISPTTLTRSNRKEITFFINGRPIQDTSVTAALLKAYHTLLM
                     VGRYPIAVLFIQIDPEAVDVNVHPAKAEVRFQHPDRIFLAVQQAVRRALLAYTPIPRA
                     STFPSEYPVQMRWSPSQKSWEGIQTQDQNEEAETGIETIANPQSPQTNLQPDLPGSRM
                     GLLRLIGQIAGTYLIAEGPDGLYLIDQHAAHERVLFEQMMSQRKKHIPGQALLQPVVV
                     ELPSASVRLINEQLPL"
     gene            1478..2137
                     /locus_tag="VN363_08955"
     CDS             1478..2137
                     /locus_tag="VN363_08955"
                     /inference="COORDINATES: protein motif:HMM:TIGR01993.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyrimidine 5'-nucleotidase"
                     /protein_id="HWQ84682.1"
                     /translation="MVFSTLLFDLDETLYRSSTGLWDAIRVRMDRFIYERLGLPEQVI
                     PQLREQYYRQYGTTLRGLQIHHDVDSDDYLAYVHDLPLKDYLQPDPTLQALLGHLPQP
                     CWIFTNADSAHAYRVLDILGVRDCFVGVIDVRDVHPACKPAPEAMQRALEIAGNPRPG
                     ECVFLDDSPANIAGARQFGLTTVLVGNGAHSHAATYCVANLTDLPAAHPEFWRDLVGD
                     V"
     gene            2152..2421
                     /locus_tag="VN363_08960"
     CDS             2152..2421
                     /locus_tag="VN363_08960"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84683.1"
                     /translation="MDDSRVFGVICITLVLVIALNVGIYLAFSKRKPDSFTSTFGKTA
                     RELRNPWQEQDASMTELSNLVADLRNNAPRPDSDGAHQDQQEHHG"
     gene            2414..3211
                     /locus_tag="VN363_08965"
     CDS             2414..3211
                     /locus_tag="VN363_08965"
                     /inference="COORDINATES: protein motif:HMM:NF013323.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="malonate decarboxylase subunit alpha"
                     /protein_id="HWQ84684.1"
                     /translation="MDKITSLNEAVKRVESGTMLALGGMTLYRRPMAFVRSLLHRYKS
                     EHAPDDLTLLAFTAGLESDLLVGAGLVKRTRSCYFGLEIFGFAPMFTHYANRGELLII
                     EETETSLALGLRAQMAGVGFMPGRAWTGTDLPRLRPDVATIRDPYSGEELMAFPAIHP
                     DIAVIHALQADSHGNASLGDNRGIDEELALTADLVIITSEEIVPELNKADIVAPFVDL
                     VVHAPNGAAPTSCYPLYPLDGQALLEYTEQVSEPASFEAYLERLLQD"
BASE COUNT          750 a          820 c          876 g          813 t
ORIGIN      
        1 aggggaagct gttcatttat caggcggaca ctggcagaag gcagctcaac caccactggc
       61 tgcagcagag cctggccagg gatatgtttc ttacgttggg acatcatttg ttcgaacaat
      121 acccgctcgt gcgcggcatg ctgatcgatg agatataaac catccgggcc ttcggcaatg
      181 aggtatgtac cggcaatctg gccgatcaga cgaagcagac ccatccttga gccaggtaaa
      241 tcgggctgga ggttggtttg aggtgactga ggattagcta tcgtttcaat gccagtttca
      301 gcttcttcat tctgatcctg tgtctggatg ccctcccagg atttttgaga ggggctccag
      361 cgcatttgga ccgggtactc ggaaggaaaa gtactcgcgc gtgggattgg tgtataagcc
      421 agtaatgccc gccggacggc ttgttgaaca gcaaggaata ttcggtcagg atgttgaaaa
      481 cgcacttccg ctttagccgg atgcacattc acgtcgactg cctccggatc aatctgaata
      541 aataatactg cgatcgggta tctacccacc attaaaagag tatggtaagc cttaagcaag
      601 gcggctgtca ccgatgtatc ctggattggg cggccattaa taaaaaatgt gatttcttta
      661 cggttcgagc gcgtcagggt cgtgggactg ataaaacccg ttactgtaat ctctggatct
      721 tcggagatta cctcaagcat ttctctggca acatcaacgc cgtacagggt tgccaggatc
      781 tcgcgccggt tgccatttcc gctggtctgt agaacaggac gaccatcttg ctgcaggctg
      841 aaacgcacct gcggataagc cagggcataa cgggtcacca gggtatcgat ctgccggcgt
      901 tcagtggtat cgtgtttgag gaacttcaat ctggcgggga cattatgaaa aaggttctcc
      961 accctcacga cagttccaac gggtgcgcca accgtctcga ctggattcat ctggccgcct
     1021 tcgccccgca ggcgagtgcc attggcagac tgatcagttc gggaggtaat cgtaaaacgc
     1081 gataccgaag ctatcgaagc cagggcctca ccccgaaaac ctaaggattg gatacggcta
     1141 agctcactcg ccgaagataa tttcgaggtt gcgtgccgct cgactgccag tgccagctca
     1201 gattccggaa tacctgcgcc atcgtcggca acctcgatca ggcgcttacc agcttcatcg
     1261 acatgtattt gaataaatgt gctgccagca tccagtgaat tttccagtaa ttcttttatc
     1321 accgaggcag ggcgttcaac gacttctccc gcggcaattt gagaggcaac ttctggttgg
     1381 aggatcctga ttggcatgcc tgtattatat cagcgggcga tgcctgtcag atcgatcagc
     1441 cctttcagag tgccctccaa tcccttataa ttgagggatg gttttttcaa cattactctt
     1501 tgatcttgat gagacactgt accgcagcag cacgggattg tgggatgcaa tccgtgtccg
     1561 catggaccgg ttcatttacg agcgattggg tctacccgag caggttattc ctcagctgcg
     1621 tgagcagtat tatcgccagt atggcaccac gctgcgtggg ctgcaaatcc atcatgacgt
     1681 tgatagtgat gattatctgg cgtatgtgca tgacttgccc ttgaaagatt atctgcaacc
     1741 cgaccccaca ctgcaggctc ttctggggca tttaccgcag ccgtgctgga tcttcaccaa
     1801 tgcggattca gctcatgcgt accgggtgtt ggatatactg ggagtacggg attgttttgt
     1861 gggtgtgatc gatgttcgag atgtccaccc ggcctgtaag cctgcccctg aggccatgca
     1921 gagagctctg gagattgccg gcaatccgag accaggtgaa tgtgtcttcc tggatgattc
     1981 tccagccaat atcgctggcg ccaggcagtt tgggttaacg accgtattgg taggaaatgg
     2041 ggcgcattct cacgctgcaa cctattgtgt agccaatctg accgatttac ctgccgcaca
     2101 ccctgaattt tggcgagact tggtgggcga tgtgtaagaa gaggataagg aatggatgac
     2161 agccgtgtct ttggggtgat ctgtataacc ctcgtcctgg ttattgcttt gaatgtggga
     2221 atctatctgg cattctcaaa acgcaagccg gatagtttca cttccacatt tggcaagact
     2281 gcacgggagc tgcgcaatcc ctggcaggaa caggatgcga gcatgacgga gctgtccaac
     2341 cttgttgccg acttacgcaa taatgctccc cggcctgata gcgatggagc acatcaagat
     2401 caacaggaac accatggata aaataaccag cctcaatgaa gcggttaagc gtgttgaaag
     2461 cgggacgatg ctcgctttgg ggggtatgac cctctatcgc cggccgatgg cattcgtgcg
     2521 cagcctgctc catcgttaca aatcagagca tgcaccagat gacctgacct tgttggcatt
     2581 tactgcaggg cttgaaagcg atctcctggt aggtgctggt ctggtcaaac gaacccgctc
     2641 ctgttatttt gggctggaaa tatttggatt tgcaccaatg tttacccact atgccaaccg
     2701 gggagagtta ctcatcattg aagaaaccga aaccagcctt gcgctgggct tgcgggctca
     2761 aatggctgga gttggattta tgccgggtcg cgcctggaca gggacagacc tgccgcgtct
     2821 tcgccctgat gtggctacaa tccgagatcc ttactcaggt gaagaattga tggcttttcc
     2881 cgcgatccac cctgatatag ccgtgattca tgctttacag gctgattcac atggtaatgc
     2941 gtcgctgggc gataatcgcg gtatcgatga ggagcttgcc ctgaccgcag acctggtaat
     3001 cattacttca gaagagatcg tccccgaact gaacaaagcc gatatcgttg ctccctttgt
     3061 cgatctggta gtccacgccc ccaatggcgc tgcgccaacc tcttgctatc cactgtatcc
     3121 gttggatgga caggctctcc ttgaatatac tgaacaggtc agtgagccgg catctttcga
     3181 agcttacctg gagcgtctat tacaggatta aatacatcga tttctgccat agattaagtc
     3241 acagattaat agaagtggg
//
LOCUS       DATMXK010000491         4183 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_130803, whole genome shotgun sequence.
ACCESSION   DATMXK010000491 DATMXK010000000
VERSION     DATMXK010000491.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4183)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4183)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4183
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_130803"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1784
                     /locus_tag="VN363_08970"
     CDS             <1..1784
                     /locus_tag="VN363_08970"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559330.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="trypsin-like peptidase domain-containing
                     protein"
                     /protein_id="HWQ84685.1"
                     /translation="KRNNIPRRVNMRVQAFKPFVYILVMVALVACGGSPTLAPVATEA
                     PAQATTPPTATIEPTTAPTAEPTVAPTETPASGAVHDLQDVQKAIIQIESQGTFIDPE
                     FGLVMNGAGRGSGFIIDPAGIAVTNNHVVTGAALLRVWVGGETESRNARVLGVSECSD
                     LAVIDIAGDGYEYLDIFTGPISVGMDIYVAGFPLGDPEFTLTKGVISKAKASGETSWA
                     SVDSVIEYDASTNPGNSGGPVITSDSEVIGVHYAGNASTRQAFGISRDVAQPVIDALK
                     SGNNQDTIGVNGSAVANEDGSLTGVWVSSVESGSAADKAGLVAGDIITMMENLVLATD
                     GTMADYCDILRSHEPTDTLSIQVLRWSNGEVLEGQLNGRELELAATFDPGTGNENSGG
                     EVSNDNYYIDPSASASGDVYYFTEFEDDLADWTYFLMSGKDENFSLEAESSRLRFDIQ
                     ARETYVYMVNNAIDVADVRVDTSTENLGRNANNVSLFCRYSDRGWYEFNIASNGEYSI
                     LKYDNNTQAYRALWTGGSTLINMGQEVNEYTTICQGDELTLYINGGFVKTIKDTSLKS
                     GLAGISVSSFNVTPIVVEFDAFALSVP"
     gene            complement(1889..>4183)
                     /locus_tag="VN363_08975"
     CDS             complement(1889..>4183)
                     /locus_tag="VN363_08975"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWQ84686.1"
                     /translation="AQYGKLIFGVDAAARVYFGKPATTLNLSEAVWLAMQAKNPNLPP
                     SPDDFDRILNEMQASNWISQQDREEARMPDPTSSLAADAYTSIAPGFTDYTLSRLSNR
                     YNLERILRGGLKITTSLNDILQLQVTCATYTQLNRSGTRVENSILPEDHDCQAERLLP
                     TLSGPAGSPPGQLAANVIILDPTNGQILAYVDDPNSNLDPAGLPGHPPGSLLTPFIYL
                     TSFTRGFTPASLLWDIPSEEVMAEIDNLDRQEHGPMRLRIAMANDYLVPAAQIMNRIG
                     FDNIQRTMQQFGLLPFYNDEGSLPDNLDQSILIEGGELTLLDAVQALGILANQGVASG
                     IPAANVESADDPFGTVEPVVVLDIRDTDNHQWAQVPTNRNQPILSTQLAYLVTNILSD
                     EGARWPTLGHPNALEIGRPAAVKPGTTIAGQDVWTVGYTPQMVVGVWVGRVNPENEPP
                     LSEQLSAALWHALMQFAHRDLPTQNWIKPSGITSLDVCDPSGLLPSPDCPTVVSEIFL
                     AGTEPIQVDNLYRAVQINRETGLLATIYTPSDLVEERVYLQVPPDAISWAQQTEISIP
                     PNMYDLVKSSSQDDPNAIISSPSMFSIVGNQVSIIGSAAGEDFSRYRIQIGRGLNPTA
                     WLQIGDDITKPVQEAELFEWDTQGLNGLYTLQLLVERNEQQVDTSTIQVTIDNQAPEV
                     AILYPQLNQVIELSDITIQADIEDNLRVARVRVLIDDQVLSQLDQPPYSTVWKTQPGE
                     HTLTIEAMDEAGNLSTANLRFTVR"
BASE COUNT          996 a          995 c         1168 g         1024 t
ORIGIN      
        1 caaaacggaa taatatccca aggagagtta atatgcgcgt ccaggcattt aaaccatttg
       61 tgtacatcct ggttatggta gcattggtcg cttgcggcgg gagtcctacc ctggctccgg
      121 tagctacgga agcccctgct caggcaacaa cacctccaac agcgacgata gagccaacaa
      181 ctgctccaac cgcggagcca acggttgcgc caaccgaaac gcctgccagt ggcgcagtcc
      241 acgatctgca ggatgtacaa aaagcgatta ttcagattga atcccagggg acattcatcg
      301 atccagagtt tggtctggtg atgaatggag ctgggcgcgg ctcaggtttt atcattgatc
      361 cagcgggaat cgcggttacc aacaaccatg tggttaccgg tgctgcctta ctcagagtgt
      421 gggttggtgg ggaaactgag tcacgcaatg cccgggtttt aggagtctca gagtgttcag
      481 atcttgcagt tattgacatt gctggggatg gctatgagta cctggatatc tttaccggtc
      541 cgatcagtgt aggtatggat atttatgttg caggctttcc actgggtgat cctgaattta
      601 cactgaccaa aggtgtgatc tcgaaagcta aagccagcgg tgagaccagc tgggcttcgg
      661 tcgattcggt tatcgaatac gacgcttcta caaatccagg aaattccggc ggtcctgtga
      721 ttacgagtga tagtgaagta atcggtgtac attatgccgg caatgccagc acccgccagg
      781 catttgggat ttcacgggat gttgcccagc ctgtgattga cgcacttaag tccgggaata
      841 accaggacac gattggggtc aatggctcag ctgtggccaa tgaagatggc agcctgaccg
      901 gtgtttgggt ttcatccgtt gagtcgggtt cggcggctga taaagccggc cttgttgccg
      961 gcgatatcat tacaatgatg gaaaaccttg ttcttgccac cgatggaacg atggcggatt
     1021 actgtgacat cttgcgcagc catgagccaa ccgatacgct gagcatccag gtgctgcgtt
     1081 ggtcgaatgg ggaagtgctg gaagggcagt taaatggccg cgagctggaa ttagccgcta
     1141 ctttcgatcc cggtaccggc aatgagaata gcggcggcga agtttcgaac gacaactatt
     1201 acatcgatcc cagcgcctcc gcttccgggg atgtgtatta cttcaccgag tttgaggatg
     1261 atctggctga ctggacatac tttttgatga gcggtaaaga tgaaaatttc tctctggagg
     1321 ctgaaagcag ccgcctgcgg tttgacatcc aggcgcgcga gacctacgtt tatatggtga
     1381 acaatgccat cgatgtggcg gatgtgcgcg tcgatacatc cacggaaaac ctgggacgca
     1441 atgccaataa tgtcagcctg ttttgccgtt attcggatcg cggctggtat gaattcaata
     1501 tagccagcaa cggcgaatat tcaatcctga agtacgacaa caatacccag gcttaccggg
     1561 cgctgtggac gggtggatcg accctcatca atatgggaca ggaagtgaac gagtacacta
     1621 ccatctgtca gggcgatgag ctaaccctgt atataaatgg tggcttcgtc aagacgatca
     1681 aggacaccag tttgaagtct ggtttggcag gcatctcggt gtcatcgttc aacgtgaccc
     1741 ccattgtggt tgaatttgat gcctttgcat tgagtgttcc ctgaatagaa atctcagcaa
     1801 cgattagaca gcgggtgata tacgagtcgc ccgctgtctt tttattgcct atcaattacg
     1861 ctgaacaatt ggaaggcgaa tgactccctt agcggacagt gaagcgcagg tttgccgtgc
     1921 tgagattccc ggcctcgtcc atggcttcga tggtaagtgt gtgttctcca ggctgggttt
     1981 tccagacagt tgaataggga ggttgatcga gctgcgacaa tacctgatca tcgatcagaa
     2041 cgcgaacccg ggctacgcgt aaattatcct caatatcagc ctggatggtt atatccgata
     2101 actcgatgac ctggttcagt tgggggtaaa ggattgctac ttctggcgcc tggttatcaa
     2161 tggtgacctg aatcgtactc gtatcaacct gctgttcatt tcgctctacc agaagctgta
     2221 aggtatatag accgttaagg ccttgagtat cccactcgaa taactcagcc tcctggactg
     2281 gtttcgtaat gtcgtcgccg atttggagcc aggctgtggg gttaagtcct cgcccaattt
     2341 gaatccggta tcttgaaaaa tcttcaccag ctgcagatcc aatgatgctc acctggttcc
     2401 cgacaattga gaacatactc ggagagctga ttatcgcatt gggatcatcc tgggaggagg
     2461 atttgaccag atcgtacata tttggaggta tcgatatctc cgtctgctga gcccagctga
     2521 tagcatctgg aggaacctgg agataaactc gttcttcgac cagatctgaa ggggtgtaaa
     2581 tcgttgccag caatccggtt tcgcggttga tttggacggc acgatataga ttatctacct
     2641 gaatgggctc tgtgccggcc aggaaaatct cactgacaac cgtagggcaa tcgggtgatg
     2701 gcagcaagcc ggaaggatcg caaacgtcga gtgaagtgat gccactgggt ttaatccagt
     2761 tttgggttgg taggtcccga tgggcgaact gcatcagagc atgccataag gctgctgaaa
     2821 gttgctcaga aagaggcggc tcattttcgg gattgacgcg cccaacccag accccgacca
     2881 ccatttgagg tgtgtaacca accgtccaca catcctgccc ggcaatggta gtgccaggtt
     2941 taacggctgc aggccggcca atttccagag cattcggatg tcccagggtg ggccagcggg
     3001 cgccttcatc gctcaggata ttggtaacca gataggcaag ctgggtgctc aaaattggct
     3061 gattgcggtt cgtggggacc tgagcccatt gatggttatc tgtgtcccga atgtccagca
     3121 cgacaaccgg ctcaactgta ccaaatggat cgtcagctga ttctacgttc gccgcgggta
     3181 taccagaggc gactccctgg ttggcgagga tgcccaaagc ctgcactgca tcgagcaggg
     3241 tcaattcgcc accctctata aggattgatt ggtccaaatt atccggcaag ctgccttcat
     3301 cgttataaaa tggtagcaag ccaaactgct gcattgtgcg ctggatatta tcgaagccaa
     3361 ttcgattcat gatctgggct gcgggaacaa ggtaatcatt tgccatggct attcgaagcc
     3421 gcatgggacc atgttcttga cgatccagat tatcaatttc agccattacc tcttcggacg
     3481 ggatatccca aagcaaagaa gccggtgtaa aacctcgcgt aaacgaggtg aggtaaataa
     3541 acggggtcag gagggagcca ggcggatgtc cagggagacc agcaggatcg aggttactgt
     3601 ttggatcatc gacataagcc aggatctgcc cattggtcgg gtcgaggatg atgacattcg
     3661 cagctaactg ccctggtggg gagccagctg gaccagataa ggttggcagt agtcgctctg
     3721 cctggcagtc gtgatcctct gggagaattg agttttccac ccgggttcca gagcgattga
     3781 gctgggtata ggttgcacag gtcacctgaa gctgcaagat atcattcagc gaagtcgtta
     3841 tttttaatcc cccacgcagg attctttcga ggttatagcg attgctgagc cgggaaagcg
     3901 tgtaatcggt gaaccccggg gcgatactgg tatacgcgtc cgcggccagg cttgaagtgg
     3961 ggtctggcat ccttgcctcc tccctatcct gttggctgat ccaattggag gcttgcattt
     4021 cgttcaggat gcggtcgaaa tcatcgggtg agggcggtaa atttgggttt ttagcctgca
     4081 tagccagcca gacagcctca gataaattta atgtagtagc aggtttcccg aagtagaccc
     4141 gagctgcggc atctacccca aaaatcaact taccatattg ggc
//
LOCUS       DATMXK010000492         5474 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_43678, whole genome shotgun sequence.
ACCESSION   DATMXK010000492 DATMXK010000000
VERSION     DATMXK010000492.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5474)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5474)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5474
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_43678"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1169)
                     /locus_tag="VN363_08980"
     CDS             complement(<1..1169)
                     /locus_tag="VN363_08980"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558902.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="BMP family ABC transporter substrate-binding
                     protein"
                     /protein_id="HWQ84687.1"
                     /translation="MQSKQLWRIFALLTMLAMIISACGTAATPTAVVTEEAAATEEVA
                     VTEAVAEPTEVVGGFQIPEVEEGKFNVGMVLIGPHDDGGWSQSHYEGLNYIQENVPNV
                     HVAYVENVPEGADSEQIFRSLSRKGFDLIFGTSYGFMDPMETVASEFPDTMYIHISGI
                     KSNETNFGNLFGAMEDMKYLAGMLAGARAKLDGNPKLGYMATFPIPEELRLGNAIALG
                     MRKTCPECTMDVRWINTWHDPIIEKEAAASLFDAGAQVVFTGADTPAVADVAQEKGKW
                     GVTYDWYGSCKVERCLTAPYWKWGPVYAGITSRVIDKTYKPGWDYFDAETGALGLLGF
                     MEGETLTKGVSELPPEVVQEVRDILAKMLAGEFTRFDVFSGPINDNAGNVVVPAG"
     gene            complement(1457..2929)
                     /locus_tag="VN363_08985"
     CDS             complement(1457..2929)
                     /locus_tag="VN363_08985"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558895.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="pyridoxal-phosphate dependent enzyme"
                     /protein_id="HWQ84688.1"
                     /translation="MPYTIDLKIEDDRLQRNVQRARERKIIIPTFAQMKNPGLVPDTI
                     KQQLGNVGLWEVNPLNLFRITWHNQPVAHGGGFGGVNYIELPSSLTGTPAHIVVLVGK
                     WFPTGAHKVGAAFGCLVPRLVTGQFDPTSQKAVWPSTGNFCRGGAYDSALLASTSIAI
                     LPEGMSRERFEWLEKIAGEVIATPGSESNVKEIFDKCWELRNSGEDLMIFNQFEEFGN
                     YLWHYDVTGHAMEEVLKQIMGPRDRYRGMASATGSAGTIASGDYMKQIFPDSKIIASE
                     ALQCPTLLENGFGSHRIEGIGDKHVPWIHNSKNTDYIVAIDDNAVVNLSRLFNEPEGQ
                     AYLIKKGVPEAIVSQLNLMGFSGISNMLSAIKFAKYNELGEEDIVLTVLTDSMELYSS
                     RLKEMHAEYGEYSLTDAAAHFAQYLHGESTDNMLELTYPERRRVHNLKYFTWVEQQGR
                     TYEEIQAQWYDRDYWTDFQRQLPEIDELITEFNQRVGLLK"
     gene            complement(2969..3928)
                     /gene="arcC"
                     /locus_tag="VN363_08990"
     CDS             complement(2969..3928)
                     /gene="arcC"
                     /locus_tag="VN363_08990"
                     /EC_number="2.7.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558558.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008804 - carbamate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006520 - amino acid metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="carbamate kinase"
                     /protein_id="HWQ84689.1"
                     /translation="MTDKKVAVVAIGGNSLIKSKQHQTVEDQYQAAKETTHHIADMIE
                     AGWDVAIGHGNGPQVGFILRRSEIAAKVEGMHEVPLDVCGADTQGAIGYALQQTLQNE
                     LFARGIQKPVATVITQVLVDHSDPAFRSPSKPIGGFMDEDEAKRRASEMGWTVVEDAG
                     RGWRRVVASPIPKEIVELDSIRILVEAGVVAISVGGGGIPVIDAGDNMYQGTAAVIDK
                     DFASSLLAQKLNADLFLISTAVEKVALNYGKPDQIWLDHITLAQARQYLAEGIHFAKG
                     SMAPKIQAIIWYLESGGKEALITNPENIGRALRGETGTRITLD"
     gene            complement(3933..4913)
                     /locus_tag="VN363_08995"
     CDS             complement(3933..4913)
                     /locus_tag="VN363_08995"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558557.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ornithine carbamoyltransferase"
                     /protein_id="HWQ84690.1"
                     /translation="MQTDLRNRDLIGDLDFSKEEVETVLDVAFDLKRKRALGESHAVL
                     RDKVLAMLFFFSSTRTRGSFEAGMAQLGGHGAFIESKTTQISHGDTPKEIGEIFGRYF
                     DGIAIRHVDWGIGNGYLNAVAAASRVPVLNMQCDIYHPFQCLADLMTIIEKKGRDLRR
                     KKMVVSWAYAASYQKPMSVPQSLVLQMPRFGMDVVLAHPPEFKLMPEIMEQAQEQARK
                     FGAGFEVVDDMEAAFKDADIVYAKSWGAMVHTPDPAEGARIIEKYKHWITDERKMALA
                     KADAIYMHPLPADRNIEVTDGVMDGPNSVVYDEAENRLHAQKAVMALTMR"
     gene            complement(4929..>5474)
                     /locus_tag="VN363_09000"
     CDS             complement(4929..>5474)
                     /locus_tag="VN363_09000"
                     /inference="COORDINATES: protein motif:HMM:NF012412.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84691.1"
                     /translation="GEHPTQILTDLFTVLTEKGRIDGLKWLAVGDMRMRTMHSLGYAL
                     TQFDCPITFVAPPDMSLTDEFKAELKEYSLNFKEAEHVEQAISEADVILVEPVVQPDY
                     TQSRQEAGEHGMTPANYKITRELLETKAKPDAILLHSLPRMDEIPPDVDITRWSRYWQ
                     EAYNGVVMRMALLALVLGAME"
BASE COUNT         1167 a         1520 c         1485 g         1302 t
ORIGIN      
        1 tcgccagcag gtacaaccac attcccggca ttgtcattga tcgggccgct gaagacgtcg
       61 aagcgggtaa attcacccgc cagcatcttg gccaggatat cgcgtacttc ctggacgact
      121 tcgggcggca gctcagaaac accctttgtc agggtctcac cttccatgaa acccagaaga
      181 cccagcgcgc cggtctcggc atcgaagtaa tcccagccag gtttgtaggt cttgtcgatc
      241 acccgactgg tgatgcctgc atacaccggg ccccatttcc agtagggggc agtcaggcag
      301 cgttccacct tgcaggagcc gtaccaatca taggtcacgc cccatttgcc tttttcctgg
      361 gcgacgtcgg ctaccgccgg ggtatcagca ccggtgaaaa ctacctgagc gcctgcgtca
      421 aacaaggaag cagccgcttc cttttcaata ataggatcgt gccaggtatt gatccagcgc
      481 acatccatgg tgcattccgg gcaagtcttg cgcataccca gggcaatggc atttccaagc
      541 cgcagctctt ctgggatcgg gaaggttgcc atataaccaa gtttcgggtt gccgtccaat
      601 ttcgcccggg caccagccag cataccagcc aggtatttca tatcttccat ggctccgaac
      661 aggttgccaa agttggtctc gttcgattta attcccgaaa tgtgaatata catggtatcg
      721 gggaattcgc tcgcaaccgt ttccatggga tccatgaatc cataggaggt gccaaaaatc
      781 agatcgaagc ctttgcggga aaggctgcgg aaaatctgtt ccgaatcggc gccttctggt
      841 acattctcga cataggctac gtgtacattc ggcacatttt cctggatata gttcaagcct
      901 tcataatggg actgcgacca gccgccatca tcatgcgggc caatcagaac catgcccaca
      961 ttgaacttgc cttcttcaac ttccggaatt tgaaagccgc ccactacttc ggtaggttca
     1021 gcaaccgctt ctgttacggc gacctcttca gtagctgcag cttcttcggt tacaacagct
     1081 gttggtgtgg ctgcggttcc gcaagctgag atgatcattg cgagcatggt aagcaaggcg
     1141 aagatccgcc agagttgttt gctttgcatt ttttcttctc ctcaattaat tgaacggtga
     1201 atgggatccg atttctaacc aactattcga ttgacttcag atctcacctc ctttggactt
     1261 taggatcgat ccgcgctggc tggacagcca ggattacagt agaagatgga cgggaccgat
     1321 ttccgattgt ccacatgtat gtaacaggca aacccataac ccgaagtttt gtgaattata
     1381 gactggaatt tgccctgcta caacccaaaa ataaataaat aggggctgag ttttcactca
     1441 acccccgggg aacctgttat ttcaacaatc ccactctctg attgaattcc gtgatcaatt
     1501 catcgatctc cggcagctgg cgctggaaat ctgtccaata atcccgatcg taccactgcg
     1561 cctggatctc ttcgtaggtc cggccttgct gctcaaccca ggtgaagtat ttaagattgt
     1621 ggacgcgccg gcgctctgga taagtcagct ccagcatgtt gtcagtcgat tcgccatgca
     1681 agtactgggc aaagtgagcc gctgcatctg tcagcgaata ttctccatat tcggcgtgca
     1741 tctctttcaa gcggctggag tacagctcca tcgagtcggt cagcacggtg agcacgatgt
     1801 cttcttcacc cagctcgtta tacttggcaa atttaatcgc cgacagcata ttcgagatac
     1861 cagagaagcc catcagattc agctggctga cgatcgcttc ggggacgccc tttttaatca
     1921 gataggcctg cccttcgggt tcgttgaaca atcgagagag attgaccaca gcgttgtcgt
     1981 caatcgcaac gatataatcg gtgttctttg aattgtgaat ccatggcaca tgcttatcgc
     2041 caataccttc gatgcggtgc gacccaaagc cattttccag cagggtgggg cactgcaggg
     2101 cttcactggc gatgatcttg ctgtcgggga agatttgctt catgtaatcg ccgctggcga
     2161 tggtgccggc agagccggtt gctgaggcca tgccgcggta gcggtcacgc ggccccatga
     2221 tttgtttgag cacttcttcc atggcgtgcc cggttacatc gtagtgccag agatagttgc
     2281 caaactcttc aaactggttg aagatcatca aatcttcacc cgaattacgc agctcccagc
     2341 atttgtcgaa gatctctttg acgttcgatt cgctgcccgg tgtggcaatc acttcgccgg
     2401 caatcttttc cagccactcg aaacgctcgc ggctcatccc ctcgggtaag atggcgatgg
     2461 atgtgctggc caataatgcc gagtcatacg cgccgccccg gcagaaatta cctgtggaag
     2521 gccagactgc cttctggctg gttggatcaa actgcccggt gaccaggcgc ggcaccaggc
     2581 agccaaaggc tgcgccaact ttgtgggcgc cggtggggaa ccattttcca accaggacta
     2641 cgatgtgcgc cggcgtgccg gtcaggctgg agggcagctc gatatagttc acaccgccaa
     2701 acccgccccc atgcgccacc ggctggttgt gccaggtgat gcggaaaaga ttaagcgggt
     2761 taacttccca cagaccgaca ttgccgagct gctgcttgat cgtatccggg accagtccag
     2821 gatttttcat ctgggcaaat gttgggatga tgatcttgcg ctcacgcgct cgctgcacgt
     2881 tgcgctgcaa gcggtcgtct tctatcttaa ggtcaattgt gtaaggcata taaaattttc
     2941 ctgattcgca gatccgtcac acgggtgctc agtccagggt gatgcgcgtg ccggtttccc
     3001 cgcgcagggc gcggccgata ttttccgggt tggtaatgag ggcttcttta ccacctgatt
     3061 caagatacca gataatagcc tggatcttag gcgccatcga gcctttggca aagtgaatgc
     3121 cctccgctag gtactgcctg gcctgggcaa gcgtgatgtg gtcgagccag atctggtcgg
     3181 gcttgccata gttgagggcg accttttcta cggcagtcga gatgaggaat agatcagcgt
     3241 tgagtttttg ggccaggagc gacgaggcga aatctttgtc gatgactgcc gcggttccct
     3301 ggtacatatt atctccagca tcgatgaccg gtatgccacc accgcccacc gaaatcgcta
     3361 ccaccccggc ttcgaccaga atccggatcg aatccagctc gacaatctct ttgggtatcg
     3421 gcgatgcaac cacgcgccgc cagccgcggc cggcatcttc gacgaccgtc caacccattt
     3481 ccgaggcgcg gcgtttggct tcgtcttcat ccataaagcc gccgatcggc ttgcttggac
     3541 tgcgaaaagc cggatcagaa tggtccacca ggacctgggt gataaccgtg gcaaccggtt
     3601 tttgaattcc gcgggcaaaa agctcgttct ggagggtttg ttggagcgcg taacctatcg
     3661 cgccctgtgt atcggcaccg cagacatcca ggggaacttc gtgcatgcct tcaaccttgg
     3721 cggcaatctc ggagcggcgc aggataaagc cgacctgcgg cccattgcca tgcccgatgg
     3781 cgacgtccca tccggcctcg atcatatccg cgatgtggtg ggtagtctcc ttagcagctt
     3841 gatactggtc ttcgaccgtc tggtgctgct tggatttaat caaggaatta ccgccaatgg
     3901 cgacaaccgc aacttttttg tctgtcatca aattacctca ttgtgagggc cataacggcc
     3961 ttctgagcat gaagtcggtt ttcggcttcg tcataaacga ccgaattggg accatccatt
     4021 acgccgtcgg tgacttcgat gttgcggtcg gcgggcaggg gatgcatata gatcgcatct
     4081 gctttggcca gtgccatctt gcgttcgtcg gtgatccagt gtttatattt ctcgatgatc
     4141 cgggcgcctt cggccgggtc gggcgtgtgg accatcgccc cccaggattt tgcatagacg
     4201 atatcggcat ctttaaaggc tgcttccata tcatccacga cctcgaatcc tgccccaaac
     4261 ttgcgcgcct gttcctgggc ctgctccata atttcgggca tgagcttgaa ctcgggtggg
     4321 tgcgccagga caacatccat gccaaagcgc ggcatttgca gcaccaggga ttggggaacg
     4381 gacatcggct tctggtaaga tgccgcatat gcccaggaga cgaccatctt cttccggcgc
     4441 aggtcacggc ccttcttctc aatgatggtc atcaggtcgg ccaggcactg aaaggggtgg
     4501 tagatatcgc actgcatatt cagcacaggg acgcgggaag cggccgcaac ggcgttgaga
     4561 tagccattgc cgatccccca gtctacgtgg cggatggcga tgccgtcgaa atagcgccca
     4621 aatatctcgc cgatctcttt gggtgtatcg ccatgcgaga tctgggtggt cttggattca
     4681 atgaaggcgc catgcccgcc gagctgagcc ataccggctt caaaagagcc gcgggtgcgg
     4741 gtggaggaga agaagaacag catggcgaga accttgtcgc gcaatacagc gtgcgattca
     4801 cccagggcgc gcttgcgttt gaggtcaaat gccacgtcga gaaccgtctc gacttcttcc
     4861 ttcgaaaagt cgagatcgcc aattaagtca cggttgcgga gatcagtttg catcggttta
     4921 cctctggttt attccattgc gcccaggaca agcgccagca gcgccatccg catcacgacg
     4981 ccattgtagg cttcctgcca gtaacgcgac cagcgggtaa tatcgacatc gggaggaatt
     5041 tcatccatgc gcggcagcga gtgcagcagg atcgcgtccg gtttggcctt ggtttccagc
     5101 agctcgcggg taatcttgta gttggccgga gtcataccat gctcgccggc ttcctggcgc
     5161 gactgggtgt aatcgggctg aacaaccggc tcgaccagaa taacatcggc ttcgctgatc
     5221 gcctgctcga catgctcggc ttccttgaaa ttcaggctgt attcttttag ctcagctttg
     5281 aattcatcgg tcaatgacat atcaggcggg gctacaaagg taatcgggca gtcgaactga
     5341 gtcagcgcat agcccaggga gtgcatggtg cgcatgcgca tatccccgac cgcaagccac
     5401 ttgagtccat cgatgcggcc tttctcggtg agtacggtga acaggtcggt gaggatctgg
     5461 gtgggatgct cgcc
//
LOCUS       DATMXK010000493         2738 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_119784, whole genome shotgun sequence.
ACCESSION   DATMXK010000493 DATMXK010000000
VERSION     DATMXK010000493.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2738)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2738)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2738
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_119784"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..184
                     /locus_tag="VN363_09005"
     CDS             <1..184
                     /locus_tag="VN363_09005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84692.1"
                     /translation="SAWTTPSKTIKTPAATPEITPGQTEITGEPADSSPVPAADSAPQ
                     ADEPQISQTKDETGTE"
     gene            188..1096
                     /gene="tatC"
                     /locus_tag="VN363_09010"
     CDS             188..1096
                     /gene="tatC"
                     /locus_tag="VN363_09010"
                     /inference="COORDINATES: protein motif:HMM:TIGR00945.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_process: GO:0015628 - protein secretion by the type II
                     secretion system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase subunit TatC"
                     /protein_id="HWQ84693.1"
                     /translation="MRNFLRKLANILTFPFRLIFNLLRGIYRFFANAGRRISTFFAEE
                     PEDSPLPDAFAKTVQNPMGILYHLNALRIHLFRALGALVITVALSLTFVEPIMALLAG
                     PLPGGMETLTAIDVTEPLGTVMKVGLLSGFTLAFPYIAFELYLFIAPGISRHSRIMGL
                     LGIPIATLFFLAGMLFAYYVMIPAALPFLLNFMGINTIPRPSSYFSFITNLMFWIGIA
                     FEFPLVIYLLASLGILKPQALQSQWRLAIVVIAVVAAMITPTIDPVSMSLVMGPLILL
                     YFLSIGLAYLAEGGRRRRAAEQEASA"
     gene            1254..2114
                     /locus_tag="VN363_09015"
     CDS             1254..2114
                     /locus_tag="VN363_09015"
                     /inference="COORDINATES: protein motif:HMM:NF026954.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016829 - lyase activity [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CoA ester lyase"
                     /protein_id="HWQ84694.1"
                     /translation="MNARRALLYIPGDDARKIAKASTLEVDCICLDMEDGVALNRKLE
                     ARQRIAQALVEQDFGRAERLVRINPTGSGLELADLAEVLPAHPDGIVIPKLEDPEQVR
                     WVSSQISMAGYGVEQVALICMVETARGIVNLASIASADPRLQALIFGAEDLAGDIGAV
                     RTPAAWEVFYARSAVVTHAAAFGLQAIDMVYADFQDEAGLRREAHQGVEMGFGGKQVI
                     HPSQIGPVQEEFTPSDAELAHALRVVEAAAAQQAVGKGAFALDGKMVDAPVVKAAERI
                     LGRARAAGKI"
     gene            2198..>2738
                     /locus_tag="VN363_09020"
     CDS             2198..>2738
                     /locus_tag="VN363_09020"
                     /inference="COORDINATES: protein motif:HMM:NF012336.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84695.1"
                     /translation="MRPVYALAGGVSKFAKARPDKTFQAVVKEAYDQAIAELGLEFAA
                     FNRLVDGSVASYFSDHFTRQLMAGIMVQDYLGLVPKPGHRVEGGGATGGLCFQEAWKS
                     VASGHMDICVAYGFETMSHVETWKGNEFIALASDVSLDYPVGGFYSGYYAMMVTRHMK
                     EFGTTVEQMAMVSVKNHRNA"
BASE COUNT          565 a          814 c          767 g          592 t
ORIGIN      
        1 gtccgcctgg acgaccccca gtaagacaat caaaacgccg gcagctacac cggaaatcac
       61 cccggggcaa actgaaatca ccggggaacc cgctgacagc tccccagttc cagctgcaga
      121 cagtgcgccg caagctgatg agccccaaat ttcccaaaca aaagacgaaa ccggaacaga
      181 ataatctatg cgcaatttct tgcgaaaact ggctaacatc ctgacattcc ctttccgcct
      241 gattttcaat cttctgcgcg gaatttaccg cttctttgca aatgcgggcc gaagaatcag
      301 cacattcttt gcagaagaac ccgaagacag ccccctgcca gatgcttttg ccaaaaccgt
      361 ccaaaatccg atgggcatcc tgtaccattt gaacgccctg cggatccatc tcttccgggc
      421 gttgggagcc ctggtcatca cggtggcgct ttccctcacc tttgtcgaac cgattatggc
      481 tttgctcgcc ggacctcttc ccggcggcat ggaaaccctc accgcgatcg atgtcactga
      541 gccactgggg acagtgatga aggttggcct gctctcgggt tttacgctcg cgtttccgta
      601 catcgcgttt gagctgtacc tgttcatcgc ccctggtatc tccaggcact cgcggatcat
      661 gggcttgctg ggtattccca tcgcaaccct cttcttcctg gcggggatgc tttttgccta
      721 ctatgtaatg atcccggcgg ccctgccctt cttactcaac ttcatgggca tcaacaccat
      781 acctcgccca tcgtcttatt tcagcttcat caccaacctg atgttctgga tcgggatcgc
      841 atttgaattc ccactcgtga tctaccttct ggcgagctta ggaatcttga agccccaggc
      901 gctgcaaagc caatggcggt tggcgatcgt cgtcatcgcc gttgtggcag ccatgatcac
      961 cccgaccatc gacccggtct cgatgagcct ggtgatggga cctttgattt tgctgtactt
     1021 cttgagtatt ggcctcgcct acctggccga aggtggtcgc agaaggcgcg ccgcagagca
     1081 ggaagccagc gcctgagttc aggcttcgat aaaacagctg aatccgacaa acctggtcga
     1141 cgaatcaggt ttgtcattca taatcaatgt ctgcacaggc tggaggtttc cgcgatcgct
     1201 gattaagtca cctttccaga tgcggtctcc ccatataaaa tgagaagtaa gatatgaacg
     1261 cccgccgcgc cttactctat atcccgggtg atgacgcccg caagatcgct aaagcttcta
     1321 cgctggaggt ggattgtatc tgcctggata tggaagacgg ggttgccctg aatcgtaagc
     1381 tggaggcgcg ccagcgcatc gcccaggctc tggttgagca ggacttcggc cgcgccgaac
     1441 gcctggtgcg catcaatcct accggctcag gcctggagct ggccgacctg gcagaggttt
     1501 tacccgccca tccggatggg atcgtgatcc ccaagctgga ggatcccgag caggtccgct
     1561 gggtgagcag ccagatcagc atggctggtt atggggttga gcaggtagcc ctgatctgca
     1621 tggtagagac ggcgcggggt atcgtcaatc tggccagcat cgccagcgcc gacccccgcc
     1681 tgcaggccct gatctttggc gctgaagacc tggcgggcga tattggcgcc gtgcgcaccc
     1741 ctgccgcctg ggaggtcttt tatgctcgca gcgcagtcgt cactcatgcc gctgcctttg
     1801 gcctgcaggc gatcgatatg gtttacgctg attttcagga tgaggctggc ctgcgcagag
     1861 aagcccacca gggggtcgaa atgggctttg gcgggaagca ggtgatccat cccagccaga
     1921 tcggtcccgt ccaggaggaa ttcacaccca gcgacgcaga gcttgcccac gccttacggg
     1981 tggtggaagc cgctgctgct caacaagccg taggaaaggg cgcttttgcc ctggatggta
     2041 aaatggtcga cgctccggta gtcaaagccg ccgagcggat cctggggcgc gcccgcgccg
     2101 ccgggaaaat ctaaaccaac cgccccctgc ctgccccaac cagctgcatt cactggcgag
     2161 gcggatccat gcacagaact gtaggaggca atctgcgatg agacccgttt atgccctggc
     2221 aggaggcgtt tcgaaattcg ccaaagctcg ccccgacaaa acctttcaag cagtggttaa
     2281 agaggcctac gaccaggcca tcgccgagct aggcctggaa ttcgcggcat tcaaccgcct
     2341 ggtggatggc tcggtggcat cctatttctc cgatcatttc acccgccagc ttatggctgg
     2401 catcatggtg caggattatc tgggtctggt gcccaagccc ggacaccgcg tggaaggcgg
     2461 tggggcaacc ggggggttgt gcttccagga agcctggaaa tccgtcgcca gcgggcatat
     2521 ggatatctgc gtggcctatg gctttgagac gatgagccat gttgaaacct ggaaaggcaa
     2581 cgaattcatc gccctggcat ccgatgtttc cctcgactat ccggtgggcg gtttttactc
     2641 gggttattac gccatgatgg tcacccgcca tatgaaggaa ttcggcacga cggtggagca
     2701 aatggcgatg gtgagcgtca agaaccaccg caacgcct
//
LOCUS       DATMXK010000494         2627 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_110045, whole genome shotgun sequence.
ACCESSION   DATMXK010000494 DATMXK010000000
VERSION     DATMXK010000494.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2627)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2627)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2627
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_110045"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1154)
                     /locus_tag="VN363_09025"
     CDS             complement(<1..1154)
                     /locus_tag="VN363_09025"
                     /inference="COORDINATES: protein motif:HMM:NF017044.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pitrilysin family protein"
                     /protein_id="HWQ84696.1"
                     /translation="MLKEIHTAPIISHWVWYHAGSRNELAGITGISHWVEHMQFKGSE
                     RFPGRGLDREIARFGGFWNAFTYLDWTAYFETLPADKIDLALQLEADRMVNSLFTPED
                     VEAERTVIISERQGHENEPMFQLSEQTQAAAFRVHAYHHEVIGDQADLETLTQADLFA
                     YYRKFYAPNNAVLAIAGDFDTPTMLARIGELYGNIPSNEPPFVRVRPEPIQRGERRVT
                     VEGPGETVYVEAAYHAPAATQADFYPLTVLDSLLSGPSSLNMFGGGISNKTSRLYRAL
                     VEKELAVSIGGGLPATIDPYLYRINAIVHPERTAEEVIQALDKQINVVQETPPGITEI
                     ARAIKQARALFAYGSESITNQAFWLGFSEMYASYDWFLNYLDNLARVTPE"
     gene            1534..>2627
                     /locus_tag="VN363_09030"
     CDS             1534..>2627
                     /locus_tag="VN363_09030"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FAD-dependent oxidoreductase"
                     /protein_id="HWQ84697.1"
                     /translation="MVTIEKADKYQVVVIGAGPAGLFAARQLALQGAHVVLLNRDIKP
                     GGLAEYGIYHNKYKMKEGLRKQFHQILELPEIDYFGNVQVGQSTGLSLEDLQGMGFQA
                     IMVTVGAQGTKWLGLPGEDLRGVYHAKDIVYHYNRLPPQSTQEFLIGKRIAIVGVGNV
                     MMDIAHWLIREQQVDEVVAVARRGPAEVKFSKKEMENIARNLDLEHLDHEFERVKPIM
                     QSVGQDANAARDFILAGLAKALEPVSKARFRLDFLASPTRLVGDTDGRVCGLEVEDTT
                     LVPQNGDTKARGLGTKRIIEADTVIFCIGDRVDEDFGLPVQWNEFVKNPNPVYPINGL
                     SYEAYDPGPGKPVEGVFVAGWSREASSGLVG"
BASE COUNT          646 a          666 c          669 g          646 t
ORIGIN      
        1 tcctcaggag tcaccctggc taaattatct aaataattca agaaccaatc ataagaagca
       61 tacatttccg aaaaacctag ccagaatgct tgattggtaa tgctttcgct gccataagca
      121 aagagtgccc gcgcctgttt aatcgctcga gcaatctcgg ttatgcctgg aggtgtttct
      181 tgaaccacat taatctgctt atccaaagcc tggatcacct cttcggctgt gcgttctgga
      241 tgcacgatcg cgtttattcg atacaggtag ggatcaattg tcgctggcag gccgccgccg
      301 atactgactg ccagctcttt ttccaccagg gcgcgataca ggcgggaggt tttgtttgag
      361 attcccccgc caaacatatt cagcgagctc ggaccgctca acaggctgtc caggactgtt
      421 aaggggtaaa aatcagcctg agtggctgcc ggggcatgat aagccgcttc cacataaacc
      481 gtttctccag gcccttctac tgtaacccgc cgctctcccc gttggatggg ttcaggtcgc
      541 acgcgtacaa aaggcggctc gttggacggg atgttcccat acagctcacc gatccgcgcc
      601 agcatggttg gggtatcaaa atcacctgcg attgccagca cagcattatt tggtgcataa
      661 aattttcggt aataagcaaa caaatcagcc tgagtcagag tctccaggtc tgcctgatcg
      721 cctatcacct catgatggta agcatggacc cgaaacgcag ctgcctgggt ttgctcgctt
      781 agctgaaaca taggctcatt ttcgtgtccc tggcgttctg agatgatgac tgtccgttct
      841 gcttcaacat cctcaggggt gaaaagactg ttaaccatcc ggtcggcttc cagctgcaat
      901 gccagatcga ttttatcggc tggcaaagtc tcaaaatagg ctgtccaatc cagataagta
      961 aatgcattcc agaacccgcc gaaccgggca atctcgcgat ccagaccgcg tccaggaaag
     1021 cgctcgcttc ccttgaactg catatgttcg acccaatggg aaataccggt gatccccgcc
     1081 agttcattgc gcgatcctgc gtgataccac acccagtggc tgataatggg cgcggtatgg
     1141 atttctttta acaatacttg aagaccattc tcaagcttta tcctgcttat tccatccggc
     1201 atgcataacc tcgtcttttc aaatgttaag ctcagctccc cacaagggga aaataccgtt
     1261 ggtcatattt tcccatgaag tattcactat agcacattct aagctatgag tgaatagtga
     1321 gctcgttttc cctttccctc acccgcggct cttgcagctt tcgcggcaag tcccggcatg
     1381 tcaggtccag gcgcgccagg tacttgtcca acccatgtcc tgtgtttata atgctatagg
     1441 aagcactctt cctgaaaacc ttcttgctga aaacacgtcc tgaccctaga aaccaggttg
     1501 aatttagagt aaagaacctg tttatgagga ggcatggtga cgatcgaaaa agccgataaa
     1561 tatcaagtcg ttgtaatcgg agctgggccg gcaggcttat ttgccgccag gcagcttgct
     1621 ttgcaaggcg cgcatgtcgt tttgcttaat cgcgatatta aacccggtgg actggcagag
     1681 tatggtattt accacaacaa atacaaaatg aaagaaggct tgcgcaagca attccatcag
     1741 atccttgaac tccccgagat cgattatttt ggcaatgtgc aggtgggtca atcaacgggt
     1801 ttgagcctgg aagatctcca aggaatggga tttcaggcta tcatggttac tgttggagca
     1861 cagggcacaa aatggctggg gctacccggt gaggatctac gtggagttta tcacgccaag
     1921 gatattgttt atcattacaa ccgcctgccg ccgcagagca cgcaagaatt cctgatcggt
     1981 aaacggatag ccattgtggg cgtaggaaac gtgatgatgg atattgcgca ttggctgatc
     2041 cgtgagcagc aggtagatga agtcgtcgca gtcgcccggc gcggtccggc agaggtaaaa
     2101 ttttctaaaa aggaaatgga aaatatcgct cgcaatctgg atctcgagca tctggaccac
     2161 gaatttgaac gcgtcaaacc gattatgcag tctgtcgggc aggacgccaa cgcagcccgc
     2221 gattttatcc tcgccggact agcgaaagcg cttgaaccag ttagcaaggc gcgcttccgc
     2281 ctggactttt tggcatcgcc aactcgtctc gttggagaca ctgatggacg cgtctgtggg
     2341 ttggaagttg aagacaccac cctcgtacct cagaatgggg acacaaaagc acgtggcctg
     2401 ggtaccaaac ggatcatcga ggcagataca gttatcttct gcatcggcga tcgggttgat
     2461 gaggattttg ggctaccagt ccagtggaat gaatttgtca agaatcccaa tccggtttac
     2521 ccaatcaatg gattgtccta tgaagcttac gatcccgggc cgggtaagcc tgtcgaaggg
     2581 gtatttgttg cgggatggtc acgggaggcc agcagtggcc tggtagg
//
LOCUS       DATMXK010000495         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_74421, whole genome shotgun sequence.
ACCESSION   DATMXK010000495 DATMXK010000000
VERSION     DATMXK010000495.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74421"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..355
                     /locus_tag="VN363_09035"
     CDS             <1..355
                     /locus_tag="VN363_09035"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWQ84698.1"
                     /translation="SMDRDMAGAVGINLNRVVALNFFLGSALAGAAGVLVSVYNNSVF
                     ATMGNLPSYKAFIIIVLGGMGSLPGVIVASLLLAVVENFMVATVGYVLPRDAIAFLVL
                     ILMLMFRPHGLLGQE"
     gene            359..1279
                     /locus_tag="VN363_09040"
     CDS             359..1279
                     /locus_tag="VN363_09040"
                     /inference="COORDINATES: protein motif:HMM:NF014684.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0015803 - branched-chain amino acid
                     transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="branched-chain amino acid ABC transporter
                     permease"
                     /protein_id="HWQ84699.1"
                     /translation="MFSSGYIISIAVFVGIWVALALSLNILVGYAGQISLGHAAFFGI
                     GAYTSAVLALRYGSPFWLDFLGAILVTGLVGIILGLPSLRVRHDFLVLATIGINFVVV
                     AIFKYVDFFGGAMGLVNIPAPTFFGTPFRGGIPYLVLVWAYALVTLGVSYYLSRTWAG
                     LAFNTVRIDEEAAAAVGVSVPRYKIYAFGISAALAGGAGSLYGHFIGSVFPDTFVFNE
                     SIGILSMVIFGGVGTLRGPIVGAILLRLAPELLRFVQDYRFTIYGALLVLMMLFQPMG
                     LLGNGSWLWKTLQGWFARRKPAARTPEVQP"
     gene            1276..2016
                     /locus_tag="VN363_09045"
     CDS             1276..2016
                     /locus_tag="VN363_09045"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84700.1"
                     /translation="MSALQVKSVSKHFGGLQALRGISFEMDNEILGLIGPNGAGKTTL
                     FNVISGFLSPSAGEVFYGGKAIHRLHPRQVVELGLARTFQIVKPFRDLTVLDNVLSGF
                     GAPIYPTAKVFLQRYHQPTVVQEAEKLLELSGLTAWERATAGELPIGLQRRLEIARAL
                     ATRPRLLLLDEPAAGLVAQEASELASLIRTLYQQEIAVIVIEHNMSFAMNLCPRILVL
                     VAGELLLDGAPDEVRANPQVINAYLGQE"
     gene            2022..>2577
                     /locus_tag="VN363_09050"
     CDS             2022..>2577
                     /locus_tag="VN363_09050"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84701.1"
                     /translation="MLEINDLDVYYGPIQAIHGLGLHVGEGEAVAVLGANGAGKSTLI
                     KTIMGLLKPKRGEIRLGGQPVTGLPSWERVARGLALVPEGGRVFRDMSVKNNLRVGAY
                     LSSEQQIKEQMEFVLELFPVLAERKDQIAKTLSGGEQQMLAIGRAMMSKPKLLLVDEV
                     SMGLMPILVRRVFEVVKDLSQRGVS"
BASE COUNT          416 a          730 c          896 g          535 t
ORIGIN      
        1 gtcgatggac cgtgacatgg cgggcgcggt ggggatcaac ctcaaccggg tggtggcgct
       61 gaatttcttc ctcggttcag ccctggctgg ggctgccggg gtgctggtga gcgtgtataa
      121 caacagcgtc tttgccacga tgggcaacct gccatcgtat aaagccttca ttatcatcgt
      181 cctgggtggg atgggcagcc tgccaggcgt gatcgtcgcc agcctgctgc tggcggtggt
      241 ggagaatttc atggtcgcta cggtcgggta cgtcctgccc cgggatgcga ttgccttcct
      301 ggtattgatc ctgatgttga tgttccgccc ccacggctta ctgggacagg agtaggccat
      361 gttttcaagc ggatacatca tcagtattgc ggtcttcgtc gggatctggg tcgccctggc
      421 tcttagcctg aatattctgg tcggctatgc cgggcagatc tccctggggc atgccgcctt
      481 ctttggcatc ggcgcctaca cctcggcggt gttggccttg cgctatggct cgcccttctg
      541 gctggatttc ctgggggcca tcctggtcac cgggctggtg ggtatcattc tcggtctacc
      601 cagcctgcgg gtgcgccacg atttcctggt gctggcgacc atcgggatca actttgtggt
      661 ggtggcgatc ttcaaatatg tcgatttctt cgggggtgcc atggggctgg tcaacattcc
      721 ggcgcccacc ttcttcggca cgcccttccg gggtgggatc ccctatctgg tgctggtgtg
      781 ggcctacgcc ctggtgaccc tgggggtcag ctattatcta tcccgcacct gggccggcct
      841 ggcattcaat accgtgcgca tcgacgaaga ggccgccgcg gcggtggggg tgagcgtgcc
      901 gcgctataag atctatgcct tcgggatcag cgccgccctg gcaggcggcg ccggctcgct
      961 gtacgggcat tttatcggca gcgtgttccc ggatacgttt gtattcaatg aatcgatcgg
     1021 catcctgtcg atggttatct ttggcggggt gggcacgctg cgcggcccga tcgtgggggc
     1081 tatcctgctg cggttggcgc ccgagctgct gcgctttgtg caggattacc gcttcacgat
     1141 ctacggcgcc ctgctggtgc tgatgatgct cttccagcca atggggctgc tgggcaacgg
     1201 tagctggctg tggaagacat tgcagggctg gttcgccagg cgcaagccgg ctgccaggac
     1261 cccggaggtg cagccatgag cgccttgcag gtgaagtcgg tcagcaagca ttttggcggg
     1321 ctgcaggccc tgcgggggat ctcgttcgag atggataacg agatcctcgg gctgatcggc
     1381 cccaacgggg cgggtaaaac cacgctgttc aacgtgatct ccgggtttct cagccccagc
     1441 gccggcgaag tgttctatgg cggcaaggcc atccaccgcc tgcacccgcg ccaggtggtc
     1501 gagctggggc tggcgcgcac cttccagatc gttaaaccct tccgcgacct gacggttctg
     1561 gacaatgtgc tcagcggttt tggggcgccg atctatccca ccgctaaggt atttctgcaa
     1621 aggtaccacc aaccgaccgt cgtccaggaa gcagaaaagc tgctggagct gagcgggttg
     1681 actgcctggg agcgggcaac cgcaggggag ctgcccatcg gtttgcagcg ccggctggag
     1741 attgcccggg cactggcgac ccggccccgg ctgctgctgc tggatgagcc tgcggcgggg
     1801 ctggtggcgc aggaagccag cgagctggcc agcctgatcc gcacgctgta ccagcaggag
     1861 atcgcggtga ttgtgattga gcataatatg tcgttcgcca tgaacctgtg cccgcgcatc
     1921 ctggtgctgg tggcgggcga gctgctgctg gatggcgcgc cggatgaagt gcgggccaat
     1981 ccgcaggtca tcaacgccta tctgggtcag gagtgaggcc gatgctggaa atcaacgacc
     2041 tggatgttta ttacggtccg atacaggcca tccacgggct gggcctgcac gtgggcgagg
     2101 gggaagccgt ggcagtcctg ggcgccaatg gcgccggtaa gagcaccctg atcaagacca
     2161 tcatgggcct gctgaaacca aagcggggag agatccgcct gggcgggcag ccggtcaccg
     2221 gcctgccgtc ctgggagcgg gtcgcccggg gcctggcgct ggtgccggaa ggcgggcggg
     2281 tgttccggga tatgagcgtg aagaacaacc tgcgggtggg cgcctacctg agtagcgagc
     2341 agcagatcaa agagcagatg gaatttgtcc tggaattatt ccccgtcctg gcggagcgca
     2401 aggaccagat cgccaaaacc ctgagcggcg gcgagcagca gatgctggcg atcgggcggg
     2461 cgatgatgag caagccaaaa ctgctcctgg tagacgaggt ctcgatgggg ctgatgccga
     2521 tcctggtccg gcgggtgttc gaggtggtga aggacctgag ccagcgcggg gtgagca
//
LOCUS       DATMXK010000496         3477 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_107348, whole genome shotgun sequence.
ACCESSION   DATMXK010000496 DATMXK010000000
VERSION     DATMXK010000496.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3477)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3477)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3477
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_107348"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..714)
                     /locus_tag="VN363_09055"
     CDS             complement(<1..714)
                     /locus_tag="VN363_09055"
                     /inference="COORDINATES: protein motif:HMM:NF024950.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SDR family NAD(P)-dependent oxidoreductase"
                     /protein_id="HWQ84702.1"
                     /translation="MMDNAYSLDRKLILVTGGGTGIGRGVALELARQGADVALSYASS
                     AKGALEAVEEIQSIGRRAIAIKGNLSEVAECRRVVDEAAAFLGGLDGLVNNAGITATV
                     DFLDVTEELFNRIYFTNIRGEYFCCQQAVPYILERGRTLQSQASEKPWAGGSIINMSS
                     VHGAVGLAGHSVYAGTKGAINAFSRELAVELCPKHIRVNVLAPGSIEVPSYFTSDPTY
                     TRAIGDSMVPWGRVGLPEDV"
     gene            complement(750..1538)
                     /locus_tag="VN363_09060"
     CDS             complement(750..1538)
                     /locus_tag="VN363_09060"
                     /inference="COORDINATES: protein motif:HMM:NF024874.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MBL fold metallo-hydrolase"
                     /protein_id="HWQ84703.1"
                     /translation="MKNLALNILETQVDPASLAIFWISQAGFVFKTASGKIIYIDPYL
                     TDYVERSLPEYGMGFKRIMGKTIEAEEVDADLIISTHSHQDHFDFDAVPILLNNPRIN
                     FVGAPDCQELYEQCGVPEERYTILHEGETLDYGDFTVSGVFADHGEMTPDALGILLDF
                     NGIKVWQVGDTAYRPDKWQDLFQKGIDILLPPINGAFGNLNGKEAAMLARDAGVKMAI
                     PCHFWMFALHYGNPMEFLEACKELAPGVQPYLMTQGERLIYTRS"
     gene            complement(1566..3125)
                     /locus_tag="VN363_09065"
     CDS             complement(1566..3125)
                     /locus_tag="VN363_09065"
                     /EC_number="7.5.2.-"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sugar ABC transporter ATP-binding protein"
                     /protein_id="HWQ84704.1"
                     /translation="MGETYLLEMNHVTKRFPGVVALKDVTLKVQQGTIHGLVGENGAG
                     KSTLMKILSGTYPTGSYEGELKLNGNLLDLHSSANALEQGIGIVPQETFVVEQLTVAE
                     NIVIGKWIDGNGKLVSMRGINKRVSIFLDNYGIDLIPGKLVTSLTAAEKQLVMIARAL
                     YRKPSVLILDEPTSSLALHEIQNLFERLEELRKKGVTCVFITHKLAEIFKITDRTTVL
                     RDGEVTGEFKRENYNEMDVISAMVGRKIENLYPTRESPIGAELVLEVHNLTVPHPTIA
                     NRNIVDNVSFSLKRGEILGLAGLVGSGRSEVVNALIGRMKKSGKVVVEGKPVVINSPA
                     DAKKVGIALITEDRKKDGLLPNMEIRPNVTLHSLEKFTSMGFLRNSSKEKTIAAEHIK
                     KFNIKTPSIEQMVTNLSGGNQQKVILAKVLMANPAILLMDEPTKGVDIGAKNEIYKLM
                     LELVAQGISIVMISSELPELLAMCDRFVVLAEGKVADEFSKAGASEHRVMLAATQARE
                     QVDSIRMETIG"
     gene            complement(3292..>3477)
                     /locus_tag="VN363_09070"
     CDS             complement(3292..>3477)
                     /locus_tag="VN363_09070"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84705.1"
                     /translation="VYGLVAQPLYEEFYASVELVAKAINGEAIEYLNLLPAPVIMEAD
                     LESYYGFNDRAEALIDQ"
BASE COUNT          826 a          975 c          795 g          881 t
ORIGIN      
        1 cacgtcttct ggtaagccca ctcgccccca ggggaccatg ctgtcgccaa tggcgcgggt
       61 ataagtcggg tcagacgtga agtagctggg tacttcgatc gaacctggag ccagcacatt
      121 gactcggata tgcttcgggc acagctccac cgccagctcc cggctgaagg cattgatagc
      181 gcccttggtg ccagcataca cagaatgccc cgcgaggccc actgccccat gcaccgacga
      241 catattgata atgctgcctc ctgcccaggg tttttctgat gcctggcttt gcaaagtgcg
      301 tccacgctct aaaatataag gtacagcctg ttggcagcag aagtattcgc cgcgaatatt
      361 ggtgaagtaa atccgattaa acagctcttc agtcacgtcc aggaaatcaa ctgtcgccgt
      421 aatcccggca ttattcacca gcccatccaa cccgcccaag aaggctgccg cttcatctac
      481 cacacggcgg cattcggcaa cttcactaag attacccttg atagcaatcg cgcggcggcc
      541 tatactctgt atttcttcca ctgcttccag ggcacctttg gcgctgctgg cataactcaa
      601 ggccacatca gcgccctggc gcgccagctc cagggccacg ccgcgcccaa ttccggtgcc
      661 tccgccggtg accaaaatca atttcctatc cagactgtac gcattgtcca tcattaataa
      721 aaactcctct gtccatgcag gtgggtaaat caggatcttg tatagatcag gcgctcgccc
      781 tgggtcatca aatatggctg cactcctgga gcaagctctt tacaggcttc caagaattcc
      841 atcgggttgc cataatgcag ggcaaacatc cagaagtggc atgggatagc catctttacc
      901 ccggcatcgc gcgccagcat ggcggcttct ttcccattca agttgccaaa ggcgccatta
      961 atgggtggga gcaggatgtc gatacctttt tggaagagat cttgccattt atccgggcgg
     1021 taggcggtat cccccacctg ccagaccttg attccattga aatccaacag gatgcccagg
     1081 gcatccgggg tcatctcacc atgatcggca aagacaccgc taacggtaaa gtcgccataa
     1141 tcgagagttt caccctcgtg cagaatcgtg taacgttcct caggtacacc acactgctca
     1201 tacagctcct ggcaatctgg cgcgccgacg aaattgattc ggggattatt cagcaaaata
     1261 ggcacagcat caaaatcaaa atgatcctgg tgggaatgtg tgctgatgat gagatcagca
     1321 tcaacttctt ccgcctcaat ggtctttccc atgatgcgct tgaatcccat gccatactcc
     1381 ggcaggctgc gttccacata gtccgttaaa tacgggtcga tgtagatgat ttttccagat
     1441 gcggttttaa aaacaaaacc agcctgcgaa atccaaaata tcgccaggct ggccggatcc
     1501 acctgtgtct caaggatatt caaggccagg tttttcattt tggatcgcct cctattcctg
     1561 cttttctacc caatggtctc catacggata ctgtcaactt gctccctggc ctgggtggcg
     1621 gccaacatta ccctgtgttc gctggcaccg gctttggaaa attcatcggc cactttacct
     1681 tcagccaaca ccacaaagcg gtcgcacatt gccagcagtt caggcagctc ggacgagatc
     1741 ataacgattg aaatgccctg ggctactaat tccagcataa gcttgtaaat ttcatttttt
     1801 gccccaatat ccacgccttt ggtcggctca tccatcagta agatcgccgg attcgccatc
     1861 agcacctttg ccaggatcac cttctgctgg ttaccgccgc tcaggttggt gaccatctgc
     1921 tcaatcgagg gcgttttgat attaaatttc ttgatatgtt cagctgcgat cgtcttttct
     1981 ttactggagt tgcgtagaaa acccatcgaa gtaaattttt ccagactgtg cagggtcaca
     2041 ttggggcgga tttccatatt tggcagcaat ccgtctttct tgcggtcttc tgtgatgagg
     2101 gcaatgccca cttttttagc atccgccggg ctgttgatga ccaccggctt gccttccacg
     2161 accaccttac cactcttttt catccgccca atcagggcgt tgaccacttc actgcgcccc
     2221 gaaccgacca ggccggccag ccccaggatc tcgccgcgct tcaaactgaa gctcacatta
     2281 tctacaatat tgcgattggc aatcgtggga tggggtactg tcaggttgtg aacttccaag
     2341 acaagttcgg cgccgatagg ggattcgcga gttggataca ggttttcaat cttgcgtcca
     2401 accatcgccg agatcacatc catttcattg tagttttcgc gtttaaattc accggttact
     2461 tctccatcgc gaagaaccgt agtacgatct gttatcttaa atatttcagc caatttgtga
     2521 gtgataaaaa cgcaggttac accctttttc cgcagctctt cgagtctttc aaacaaattt
     2581 tgaatttcgt gcagcgccaa cgaggaggtc ggctcatcta aaatgagcac actgggcttg
     2641 cggtacagcg ccctggcaat catcacaagc tgtttttcgg cggcagtcag actggtgacc
     2701 agctttccag gaattaagtc gataccgtaa ttatccaaaa aaatacttac ccgcttatta
     2761 atcccgcgca tactgactaa tttgccatta ccatcgatcc atttaccaat aacaatgttt
     2821 tcagcaacgg tcaactgctc caccacaaag gtctcttggg gcacaatccc gatgccttgt
     2881 tccagggcat ttgctgagga atgcaggtcg agcagattac cattcagctt caattcgcct
     2941 tcgtaggacc cggtcggata ggtcccggaa aggatcttca tcaaggttga tttgccagcc
     3001 ccattttcgc ccaccagccc atgaatcgtc ccctgctgca ccttgagggt aacatccttc
     3061 aaagccacaa cccccggaaa acgtttggtg acgtggttca tttcaagcag ataggtttcg
     3121 cccatggata atcctgttca tctttcgaat caaaaccaga ctaaattacg caatggaact
     3181 cttcagtttt tgaggggata actgcatcac gcagatgcag ttatccccat tttttccaac
     3241 ccgatttagc gatgcaagca gaataggaca gtgggttgga aagcgattag attattggtc
     3301 aatcagcgcc tcagcccggt cattgaagcc atagtaggat tccaggtcgg cttccatgat
     3361 caccggggcg ggcagcaggt tgaggtattc aatcgcttcg ccgttgatcg ctttagcaac
     3421 cagctcgact gacgcataga actcttcata cagaggttgg gctaccagac cgtatac
//
LOCUS       DATMXK010000497         3424 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_4835, whole genome shotgun sequence.
ACCESSION   DATMXK010000497 DATMXK010000000
VERSION     DATMXK010000497.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3424)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3424)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3424
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4835"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VN363_09075"
     CDS             complement(<1..1194)
                     /locus_tag="VN363_09075"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561359.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0004812 - aminoacyl-tRNA ligase activity
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0043039 - tRNA aminoacylation [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="amino acid--tRNA ligase-related protein"
                     /protein_id="HWQ84706.1"
                     /translation="MTYSYSNLEQNRLEKLENLRQSGHEPYPTQAERTHTSQEAIQAF
                     ETAEAAAVAAPNPQPVMAVLAGRLRSMRTMGKITFAHIEDGYGRIQLFLRANDLGPET
                     LELFNKEFDLGDFIQASGEMFRTRSGEITLRVDSFRMLSKAITPLPAAKDQVVDGELK
                     QYATLSDPETRFRQRYADLAVNKDVRQIFVTRAAIVRALRDFLDQNDFLEVETPILQP
                     IYGGAAARPFVTHHNQLKQDLYLRISFELYLKRLLVGGYERVYEIGRDFRNEGVDRTH
                     NPEFTQLEFYMAYADYEKVMEFTEQMLVFTAERVLGKRSLTYQGRTISLEPPWQRMDL
                     SEGLLEVTGIDIHAHPTAESLAAAMRIKDIPADPKAARGKLIDSLLGDFLEPTLLAPT
                     FLYDYP"
     gene            complement(1282..1743)
                     /gene="greA"
                     /locus_tag="VN363_09080"
     CDS             complement(1282..1743)
                     /gene="greA"
                     /locus_tag="VN363_09080"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013561358.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0070063 - RNA polymerase binding [Evidence
                     IEA];
                     GO_process: GO:0032784 - regulation of DNA-templated
                     transcription elongation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription elongation factor GreA"
                     /protein_id="HWQ84707.1"
                     /translation="MPPSYLTRQGYEKLQDELEYLRSTKRQEVAVRLHEAMEGGELIE
                     DAEYEAAKNEQAFVEGRIQELEMLLANARIVEESGKREIVQVGAKVSIQEEEAEPETF
                     TIVGPAEANPRLGRISHESPLGRALMDHRAGDKVKVDAPDGAFFVQILKVE"
     gene            complement(1911..2657)
                     /locus_tag="VN363_09085"
     CDS             complement(1911..2657)
                     /locus_tag="VN363_09085"
                     /inference="COORDINATES: protein motif:HMM:NF014625.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PIG-L family deacetylase"
                     /protein_id="HWQ84708.1"
                     /translation="MDWVFLSPHLDDVAISCAGLVWELTQQDHPVSIWSICAGKPPAG
                     PLSPFAQSLHARWNTGSQTVRTRRAEDQRSCLRMGAQPVYLDIPDCIYRTARKTNAHL
                     YPSEESLFGPVHPAEARLIQHLSRRLLRSLPEDAQIVCPLALGSHVDHQLTRLSAEQT
                     GRSLLYYADYPYAHQAENELASLQLAGWQPQLWPVSPEALQEWIAAVWEHRSQISTFW
                     SGLEDVHSHIAAYCQQMGGVRLWSSPPKAA"
     gene            2774..3223
                     /locus_tag="VN363_09090"
     CDS             2774..3223
                     /locus_tag="VN363_09090"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84709.1"
                     /translation="MDRSDNSRFRLRFILIAITLATIPCYCAGIFMVYQADRVANLPT
                     FTPTLTFTATLTSTITPTVTATTTLFPSQTPLTPTLTFTPTPTVTLTPTITLTPTPTL
                     TPTITLTASPSPTPTETPTQAPPPPSVTPTPPDVTPPPDEDAPTPTS"
BASE COUNT          640 a          988 c         1082 g          714 t
ORIGIN      
        1 cgggtaatcg tacaggaagg tgggggcgag cagggtgggc tcgaggaagt cgcccagcag
       61 ggaatcgatc aatttgccac gcgccgcttt ggggtcggcg gggatgtctt tgatgcgcat
      121 ggcggctgcc aggctctcgg cggtagggtg agcgtggata tcgatgccgg tgacttcgag
      181 caggccctcg ctcaggtcca tgcgctgcca gggcggctcg aggctgatgg tgcggccctg
      241 gtaggtgagg ctgcgcttac ccaggacgcg ctcggcggtg aagaccagca tctgttcggt
      301 gaattccatc accttctcgt aatcggcgta agccatgtag aattcaagct gggtaaattc
      361 ggggttgtga gtgcggtcca cgccttcatt gcggaagtcg cgcccgatct catagacgcg
      421 ctcgtagccg ccaaccagca ggcgcttgag gtacagctcg aaggagatgc gcaggtacag
      481 gtcctgcttg agctggttgt ggtgggtgac gaatgggcgg gcggcagccc cgccgtaaat
      541 gggctgcagg atgggggttt cgacctccag gaagtcgttc tggtccagga aatcacgcag
      601 ggcgcgcacg atggccgccc gcgtgacgaa gatctggcgt acgtctttgt tgacggccag
      661 gtcggcgtag cgctggcgga agcgggtctc ggggtcggag agggtggcgt actgtttgag
      721 ctcgccgtcc accacctggt ccttggcggc gggcaggggg gtgatcgcct tgctgagcat
      781 gcggaaggaa tcgacccgca gggtaatttc gccgctgcgg gtgcggaaca tctcgccgct
      841 ggcctggatg aagtcgccca ggtcgaattc cttgttgaac agctcgagag tctccggtcc
      901 gaggtcgttg gcgcgcagga agagctggat gcgcccataa ccgtcttcga tgtgggcgaa
      961 ggtgatcttg cccatggtgc gcatggagcg caggcggccg gcaaggactg ccatcacggg
     1021 ttgtgggttg ggcgcggcta cagccgcggc ttcggcggtt tcgaaggcct ggattgcttc
     1081 ctggctggtg tgggtgcgct cagcctgggt ggggtaaggc tcgtgcccac tctggcgcag
     1141 gttttccagc ttttccaggc ggttttgctc gaggttggag taagagtagg tcatagtcaa
     1201 tttcctgaag aatgattgcg aataaagaaa aagccccgcg cagagtggac tcggcgcggg
     1261 gctggtgttg atgccccgag gttattctac cttaaggatc tggacaaaga aggcgccatc
     1321 cggcgcatcg acttttacct tgtcgccggc gcggtgatcc atcagggcgc ggcccagggg
     1381 tgattcgtgc gatatgcgcc ccaggcgagg gttggcctcg gccggtccca cgatggtgaa
     1441 ggtctctggc tcggcctctt cttcctgaat ggataccttt gcacctacct ggacgatttc
     1501 acgcttgccg gattcttcga caatgcgggc attggcgagg agcatttcaa gctcctggat
     1561 gcgtccttcc acaaaggcct gttcgttctt ggcggcttcg tattcggcgt cttcaatcaa
     1621 ttcaccgcct tccatcgctt cgtgaagacg gacagcaact tcctggcgtt tggtagaacg
     1681 caggtactca agttcgtctt gcagcttctc gtagccctgg cgggtcaaat atgatggtgg
     1741 catttccctg tcctaattat ttgattaaga aaaccacccc ttgcggagcg attttattcc
     1801 gaccgtcctc ttgctttata gcgaacggcg gctcaaggtt gaaaaaccct ggggagccgc
     1861 ggcatcattt tagcatagac ctggatagaa cgcaagttga cggcgaagcc ttacgcggcc
     1921 tttggcgggg aggaccacag ccggactccc cccatttgct ggcaataggc tgcaatgtgg
     1981 ctgtgaacat cttcgaggcc gctccagaag gtgctgatct gggaccggtg ctcccaaaca
     2041 gccgcgatcc actcctgcaa ggcctcagga gagaccggcc agagctgcgg ctgccagcca
     2101 gccagttgga gagatgccag ctcgttctcc gcttgatgcg cgtaagggta atcggcgtaa
     2161 tacagcagtg accggccggt ttgttcggcc gacaggcggg tgagctggtg gtcgacgtgg
     2221 ctgccaagag ccagagggca tacaatctgg gcgtcttcgg gcaggctgcg cagcaggcgg
     2281 cgcgacaggt gctggataag gcgtgcttca gcaggatgta caggaccaaa taatgattct
     2341 tcagaaggat agagatgggc gttggttttc ctggcagtgc gatagatgca gtcagggata
     2401 tccagataga caggctgagc gcccatccgg aggcaggaac gctggtcttc agcccggcga
     2461 gtgcggacag tctgtgaccc ggtgttccag cgagcgtgca ggctttgagc gaatggagag
     2521 agcggaccag ccggcggttt accggcgcag atgctccaga tgctcaccgg gtgatcctgt
     2581 tgggttaact cccagaccag gccggcgcag gatatggcga catcatccag gtgaggggag
     2641 agaaaaaccc agtccatgcc aaaagtatac ctgatttcag cggttcactg gcacttgcca
     2701 ccccttccgg ctggtgttat tatcaaacac ctgaggggtg ttccggcttt gcgggcgcct
     2761 ttgagcatac tcgatggata gatccgacaa ttcccgcttc cgtttgcgct tcatcctgat
     2821 cgcgatcaca ctggcgacca tcccgtgtta ttgtgccggg atctttatgg tctaccaggc
     2881 tgatcgggtt gccaacctgc ccaccttcac gcccacgctg accttcacag ccaccctgac
     2941 ttccacgatt accccgactg taacggcgac gacgaccctg ttcccctcgc aaacgccttt
     3001 gacgccgacc ctgaccttta cgcccactcc cacggttacg ttaaccccaa cgattaccct
     3061 gactcccacg cctactctga cgcccacaat cacgctcact gcttctccca gcccaacccc
     3121 aaccgaaacg ccgacccagg ctcccccgcc gcccagcgta acgccgaccc cgcctgacgt
     3181 cacgccaccg ccggatgaag acgcgccgac gccgacgtca taagccaggc gaattgttca
     3241 cgagctcgat gaaaggcatt caattgatta aatttcggcg cgcctcagcc atagtcctgg
     3301 ccagcctagt ctgggggctg tctgcctgta ccgggttgct ccccacggcg gtgatcccag
     3361 agccggctgt cagcgagact gcccctgcga ccgcccggcc gacacctgcc aggaccgccg
     3421 cggc
//
LOCUS       DATMXK010000498         2149 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_22485, whole genome shotgun sequence.
ACCESSION   DATMXK010000498 DATMXK010000000
VERSION     DATMXK010000498.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2149)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2149)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2149
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_22485"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(110..1174)
                     /gene="gmd"
                     /locus_tag="VN363_09095"
     CDS             complement(110..1174)
                     /gene="gmd"
                     /locus_tag="VN363_09095"
                     /EC_number="4.2.1.47"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_017944086.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008446 - GDP-mannose 4,6-dehydratase
                     activity [Evidence IEA];
                     GO_process: GO:0000271 - polysaccharide biosynthetic
                     process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GDP-mannose 4,6-dehydratase"
                     /protein_id="HWQ84710.1"
                     /translation="MKKALITGITGQDGSYLAELLLSQGYEVHGIIRRASTFNTQRID
                     HIYHDPHTQNGVNLHLHYGDVAASGNLIEIIYNVRPDEVYHLAAQSHVRVSFDLPEYT
                     GDVTGLGTMRILEAIRKSGITARFYQASSSEMFGAAKPPQNEATPFEPQSPYAAAKVY
                     AFYVTKNYRQAYNLFACNGILFNHESPRRGETFVTRKITRALAAIKAGRQNKLYLGNL
                     DARRDWGYAPDYVAAMWKMLQFDHADDFAIGTGESHSVREFLDEAFGYVNLDWKQYVE
                     IDPRYFRPTEVDYLMSDPTKAIKVLGWEPRIFFKDLVRIMVDADLELSGQPSPGEGKK
                     LLEGRHGPWHRWESQVVSMG"
     gene            complement(1211..1726)
                     /locus_tag="VN363_09100"
     CDS             complement(1211..1726)
                     /locus_tag="VN363_09100"
                     /inference="COORDINATES: protein motif:HMM:NF014417.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006354 - DNA-templated transcription
                     elongation [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA];
                     GO_process: GO:0140673 - transcription elongation-coupled
                     chromatin remodeling [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination/antitermination NusG
                     family protein"
                     /protein_id="HWQ84711.1"
                     /translation="MAELWYAVRSKPRKEDVLWKQLRSQGFEVFYPRVRVHHVNPRAR
                     KQEPYFPGYLFIQANLTDTGLSRFQWMPYSLGLVSFGGEPSAIPENLIHEIRKRVEAI
                     AAAGGEVFDGLKAGDKIYIQEGPFRGYEAIFDARLPGTERVRVLLEFLGNQRKIPVEL
                     NAGQIQQIKRK"
     gene            complement(1911..>2149)
                     /locus_tag="VN363_09105"
     CDS             complement(1911..>2149)
                     /locus_tag="VN363_09105"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84712.1"
                     /translation="SDRRAWLPLGLVAALLLGISLLAGAGPWLLTNAAPYVNRALEAV
                     ALVLAISLGLHLVLLPPFWVARQGISRVSGLEVA"
BASE COUNT          419 a          615 c          672 g          443 t
ORIGIN      
        1 gtgtataggg tttgtttgga aagtggggaa tcaggcaagt aaaataaatc cggagaagct
       61 ggctgcgaga accgcagcca gcttcgaata aatttcgacg tagtcgaaat taacccatgg
      121 acacgacctg gctttcccag cggtgccagg ggccgtggcg gccttcgagc agcttcttac
      181 cttcccccgg tgagggctgc ccggaaagct ccaggtcggc gtcgaccatg atgcgcacca
      241 ggtctttgaa gaagatgcgc ggctcccagc ccagcacctt gatcgccttg gtggggtcgg
      301 acatcaggta gtccacttct gtggggcgga aatagcgcgg gtcgatctcc acatactgct
      361 tccaatccag gttcacgtag ccaaaggctt cgtccaggaa ttcgcgcact gagtgggatt
      421 caccggtgcc gatcgcaaaa tcatcggcgt ggtcgaactg gagcatcttc cacatggctg
      481 ccacgtaatc gggggcgtag ccccagtccc gccgggcgtc caggttgccc aggtagagct
      541 tgttctgcct gccggcctta atggctgcca gggcacgggt gatcttgcgc gtgacgaagg
      601 tctcgccgcg gcggggtgat tcgtggttga agaggatgcc gttgcaggcg aagaggttgt
      661 acgcctggcg gtagttcttc gtcacgtaga aggcatacac cttggcggcc gcgtaagggg
      721 actgcggctc gaagggcgtg gcttcgttct gcgggggttt ggcggccccg aacatctcgc
      781 tcgaggaagc ctggtagaag cgggcggtga tcccactctt gcggatggct tccaggatgc
      841 gcatggtccc cagcccggtc acgtcaccgg tgtattcggg caggtcgaag cttacccgca
      901 cgtgggattg ggctgccagg tggtagacct catccgggcg gacgttgtag atgatctcga
      961 tcaggttgcc cgaggcagcc acatccccgt aatgcaggtg caggttcacc ccgttctggg
     1021 tatgcgggtc gtgatagata tggtcgatgc gctgggtgtt gaaggtactg gcccggcgga
     1081 tgatcccgtg gacctcatat ccctgggaga gcagcagctc ggccagataa gagccgtcct
     1141 gaccggtaat gccggtaata agtgctttct tcattgcttt taatctaatc cttctctgga
     1201 ggtagggtaa ttatttgcgt ttaatttgtt ggatctgtcc ggcgttcagc tcgacaggga
     1261 tcttgcgctg gttacccagg aattcgagca ggacgcgcac gcgctccgtt cccggcaggc
     1321 gggcatcgaa aatcgcttca taaccgcgga agggaccttc ctggatatag atcttatccc
     1381 ccgctttcag cccgtcaaag acttcgccac ccgcggcggc gatcgcctcc acccgcttgc
     1441 ggatctcgtg gatgagattt tccggtatgg ccgagggctc gcccccgaaa gaaaccaggc
     1501 ccagagagta cggcatccac tggaagcggg agaggccggt atccgtcagg ttcgcctgga
     1561 tgaacagata gccggggaaa tacggctcct gcttgcgagc gcgcgggttc acgtggtgga
     1621 cacgtacgcg gggataaaat acttcaaagc cctgggagcg cagctgcttc cacaatacat
     1681 cttctttgcg gggtttgctg cgcacagcat accaaagttc agccataccg ttattgatca
     1741 cctcttcaag ggtaaagttt accctactca gagggtttca tcaagcgccg cgccaacctg
     1801 gcgcgctccg cccttccgac atccgttccc ggcccgggat tgtacgcggc ttattttaac
     1861 caatggttaa gcgctctgga agtttccatg agggttgaaa ggctgtaatc tcaggcaact
     1921 tccagaccgg atacgcggct gattccctgc cgcgccaccc agaacggcgg cagcagcacc
     1981 aggtgcaggc ccaggctgat cgccaggacc agggcgacgg cttccaaagc ccggttcaca
     2041 tagggagcgg cgttggtcag cagccacggt ccggcccccg ccagcaagct gatccccagg
     2101 agcagagccg ccaccaggcc gagcggcagc caggcgcgcc ggtccgagg
//
LOCUS       DATMXK010000499         2720 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_84707, whole genome shotgun sequence.
ACCESSION   DATMXK010000499 DATMXK010000000
VERSION     DATMXK010000499.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2720)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2720)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2720
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84707"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..740
                     /locus_tag="VN363_09110"
     CDS             <1..740
                     /locus_tag="VN363_09110"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559127.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="carboxypeptidase M32"
                     /protein_id="HWQ84713.1"
                     /translation="NSAIFSTIHETGHALYEQGVDQALERTPLAGGASLGMHESQSRL
                     WENLVGRSYPFWQHFYPRLQEYFPAQLGNVSLDRFYKGINKVQPSLIRIEADEATYNL
                     HIMLRLELEIALIEGSLQVKDLPQAWNTRMQEYLGVTPPNDKNGVMQDIHWSGGLIGY
                     FSTYALGNLISAQLWERINQDIPDLENQIQRGEFSSLLGWLRTHLHRHGAKYEPQELV
                     QRITGTKIDPAPYLRYLTNKYSQVYGF"
     gene            841..1275
                     /locus_tag="VN363_09115"
     CDS             841..1275
                     /locus_tag="VN363_09115"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ84714.1"
                     /translation="MSLAAMVEFYPAWLPGMEGRPMASILFVDDDPYTLETLTKAAEL
                     FGHRAVLAGSGDEAIRLLTSERPDLIFIDLMLPDMDGLQLLKQIAPRTGADRMPVYVL
                     SAAADDNLSEQALALGAQDYLTKPLRLATFLDIIQQYLERAG"
     gene            1301..2353
                     /locus_tag="VN363_09120"
     CDS             1301..2353
                     /locus_tag="VN363_09120"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84715.1"
                     /translation="MKWILILALGFLAASLGACDFPSNEDLPTLLPAEQLPTVIAATM
                     AAYATETANELPAENTSLDPYSTIPSAELQQAGNLQPTGQEAAETADAATPEATDLSS
                     GTPSSVEATIPSGQVSAGELPVGTPFPELPIAGIQIFKPGDMSKVASPIEVSGYLRPG
                     ARGRVTIELFGEDGRLLVRQIRVYDVTPGARVNLAEDVPFQISAAAEVGRLVLSMADE
                     AGRTIALNSIDLILLSMGDSDINPSVALQEFIYIREPKLKNLVQGGSVLVSGLARPFT
                     DQPLVVHLIAEDGRVVGQRVVGVSERAANGYGEFAVEVAYTVDELTPVRLTVYEIGMP
                     VSPIRYLSSLEIVLGP"
BASE COUNT          604 a          717 c          760 g          639 t
ORIGIN      
        1 taaactcggc gatattcagc acaattcacg aaaccggcca cgctctatat gaacagggtg
       61 tggatcaagc cctggagcgc actccactgg caggtggtgc gtcgttgggt atgcatgaat
      121 cgcaatcgcg cttgtgggaa aatctggtcg ggcggtctta tccgttctgg cagcattttt
      181 acccgcgctt gcaggaatat ttccctgctc agcttgggaa tgtcagcctg gatcgtttct
      241 ataaaggcat caacaaggtc cagccatctt tgatccggat tgaagcagac gaggcgacat
      301 acaacctgca tatcatgctg cggttggaat tggagattgc gctgatcgaa ggttcgctgc
      361 aggtgaagga tctaccgcag gcctggaata cccgcatgca ggagtacctg ggggtgacgc
      421 ctcccaatga taagaatggt gtgatgcagg atatccactg gtcgggtggc ttgatcggct
      481 acttctcaac ctacgctctg ggcaacctga tttcagccca attatgggaa cgaattaacc
      541 aggacatccc cgatctggag aaccagatcc agagaggtga gtttagcagt ttattgggct
      601 ggctgcgtac ccatcttcat cgccacggag cgaagtatga gcctcaagag ctggtccagc
      661 gcatcacagg cacgaagatc gatccggctc cgtatctgcg ctacctgacc aataaataca
      721 gccaggtgta cggcttttag gctttggatt aatggcttaa tccccgggat ggatcgatcc
      781 atctcgggga tttaatttac ctcacagact tgatagccaa aagtaagggc gcctggagca
      841 gtgagccttg ccgctatggt agaattttat ccagcctggc taccgggcat ggagggccgg
      901 cctatggcga gtattttgtt tgtggacgat gacccttata cgcttgaaac cctgaccaaa
      961 gcggccgagt tattcggcca ccgggcagtc ctggctggga gtggggatga agccatccgg
     1021 ttgttaacaa gcgaacggcc tgatctgata tttattgatc tgatgctgcc agatatggat
     1081 gggctgcaat tgctgaagca gattgcaccc cgcacaggcg cggacagaat gcctgtttat
     1141 gtattatccg cggctgccga tgataatttg agcgagcagg ctctggcgct gggggcgcaa
     1201 gattatctca ccaaacccct gcgtttggca actttcctgg atatcatcca acaatacctt
     1261 gaacgcgcag gataactctc tgccgctcca gccttctcag gtgaaatgga tacttattct
     1321 ggcgcttggg ttcctggcag ccagcctggg tgcgtgtgat tttccctcca atgaggatct
     1381 acccactctc ctgccagccg agcaactgcc cacagtaatt gctgcgacga tggcagccta
     1441 tgcgaccgaa actgcaaacg agctgccggc agaaaacacc tctctggatc catactctac
     1501 aattccgtcc gccgagcttc aacaagcagg caatctgcaa cctaccgggc aagaagcggc
     1561 agagacagcg gatgccgcga ctcctgaagc gacagattta tcttctggga cgccaagttc
     1621 tgttgaggcg acaatcccca gcgggcaggt cagcgctggc gaattgcctg tcggaacgcc
     1681 tttcccggag ctgccgattg caggcatcca gatattcaag cccggggaca tgtcgaaggt
     1741 agcctccccg atcgaagtaa gcggctatct ccgtccggga gcacgcgggc gggtgaccat
     1801 tgagctcttc ggcgaagatg ggcgcctgct ggtgcgccag atccgcgttt acgatgtgac
     1861 cccgggggcg agagtgaatc tggcggagga tgtgcctttt caaatctcag ccgcggccga
     1921 agtgggacgg ttggtgctga gcatggcgga tgaagcaggc cgcacgatcg cccttaattc
     1981 gatcgatctg atcttgcttt ccatgggtga ttcggatatt aatccctctg ttgcactgca
     2041 agaattcatt tacatccgag aaccgaaatt gaaaaacctg gttcaggggg ggtctgtgct
     2101 ggtcagcgga ctggcgcgcc catttacaga ccaacctctg gtggtgcatt taattgctga
     2161 agatggccgc gtggtgggtc agcgcgtggt gggggtcagc gaacgtgcag ccaatggcta
     2221 tggcgaattt gcggtagaag tcgcatatac cgtggatgag ctgactcccg tccggttgac
     2281 cgtttatgaa attgggatgc ctgtctctcc gatccgctat ctatccagcc tggaaatcgt
     2341 cctgggaccc tgagtgccga actgctggtt gaccaaaagc aatacttcct tttacaatat
     2401 tcgcatggcc tgtgtacgca cgttgatgcc ttgggccggc tttcaccgcc ctgaccacga
     2461 cccgccggct cgcccataaa ttttccccaa ctccgcgtaa ctgcttttgg ctgccggaga
     2521 tttcctactg cgcgttttcg ctgtcactcc ggccaggaac taccccttag aattattagt
     2581 gtttgcttca ggagattgaa atggcgaacc atccttttgt atcgaagcgt gctccctatt
     2641 atgcagaaat tccagatgag aagtggaaca actggcgctg gcagctcagc aaccgcatca
     2701 atacggttga ggaattcgag
//
LOCUS       DATMXK010000500         8399 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_141645, whole genome shotgun sequence.
ACCESSION   DATMXK010000500 DATMXK010000000
VERSION     DATMXK010000500.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 8399)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 8399)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..8399
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141645"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..696
                     /locus_tag="VN363_09125"
     CDS             <1..696
                     /locus_tag="VN363_09125"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560437.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator transcription factor"
                     /protein_id="HWQ84716.1"
                     /translation="GEMSKKILVVDDAAQLRKLVKTYLEQEGYAVVTAADGQEALYVA
                     RHEKPDLIILDLMMPEMGGYEFMRAYTREADTPIILLTARVEENDKVLGLELGADDFV
                     TKPFSPRELAARVRAVLRRTAKQTAPGDILRAADITLDRAGRVVLVGEHQIDLTPSEF
                     DILAALLAAPGRAFTRLELLERLQGTAYEGYERTIDVHIRNLRAKIEPDDTQPRYVET
                     VYGIGYRFASERR"
     gene            706..2076
                     /locus_tag="VN363_09130"
     CDS             706..2076
                     /locus_tag="VN363_09130"
                     /inference="COORDINATES: protein motif:HMM:NF014567.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="HAMP domain-containing sensor histidine kinase"
                     /protein_id="HWQ84717.1"
                     /translation="MRSLAIKLTLAFLMVGIVGAVSVAFMVRAQTQRQFSQLVMDKSQ
                     QTLVTNLTQYFIANRSWSGVESVFHPAPGNFDPAPNPIEPFVERRTLFALADPTGKVV
                     FGDSRNDLEVIKSADLKRGVGIKVDGETVGTLIFVPSLNRWDRGTPEGNFLTEVSFAS
                     LRSALIASVLALGLGGVLAFTMTRSIRDLTTATTVLAQGKLGHQVKVRSQDEIGVLAH
                     SFNKMSSDLEHANQLRRQMTADIAHDLRTPLSVILGYTEALSDGKLTGSPEIYSIMHT
                     ESQHLAHLIDDLKVLALADAGEIPLTVQAVPPASLLHRTADAYKIQAEQKGITIEVET
                     APALPEVIIDHERMAQVMGNLVSNALRYTPAGGRISLSAAETGGKVSILISDTGEGIS
                     AGDLPFVFSRSFRGDQARQQENGESGLGLAIAKSLVEAQGGVISVTSQPGKGSTFRIE
                     LPVLEK"
     gene            complement(2193..2969)
                     /locus_tag="VN363_09135"
     CDS             complement(2193..2969)
                     /locus_tag="VN363_09135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84718.1"
                     /translation="MKRSSLLTSLLVLSLVLTACSSAAATGTTLQASDGTQDPGPEGG
                     LSTELKLAVGTLLLDDSEQPVNAETAAELLPLWKAARSLGSSSTITTDEYQAIFKQIQ
                     ETMPAEQMAAITAMQLTREDMLSLSEKLGIEMKGGGGGPMGTPSAEMLETLQAARESG
                     DLPEMPGGGGGLTSGGGGMPAGGGPGGGIFQRSENPAGAGMDPSMAGAGMPDPDASTS
                     AVRRGGVMQSIPTELLDAVIDNLQDKADGKIVKKYPWRPL"
     gene            complement(3063..4235)
                     /locus_tag="VN363_09140"
     CDS             complement(3063..4235)
                     /locus_tag="VN363_09140"
                     /inference="COORDINATES: protein motif:HMM:NF024116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84719.1"
                     /translation="MRSLLTMLGVIIGVAAVIIMVSISRGTEKTIEEQITTLGSNLLY
                     VQASMMRGGPGQPQTGGLVYDDAFTIAKNVAGVQGVVVEQSASGDVKGGSVTVEEVTI
                     LGTTTDFPSVRDIDVGLGRYFTDTDLDRKQKVAVLGATLAQELFGETTAIGQIVTVNN
                     VKLTVVGVMAESGITGNVDYDTYLFTPITVVFDKFNFDMFARMFGDSVRVIYVEVEDK
                     SLIDQVTYGIQVLLAKHHEVSLDSLDFVVRSQDDIIETQESTTAAFRSLLGWVAGVSL
                     FVGGIGIMNIMMVSVTERTREIGIRQSVGATPNNIRWQFLTEALLLSLVGGLIGVLAG
                     VGGSWLFGKLGDMRTLVTVSSILLAFGSAAAVGVFFGFYPANKAAQLDPIQALRHE"
     gene            complement(4276..5025)
                     /locus_tag="VN363_09145"
     CDS             complement(4276..5025)
                     /locus_tag="VN363_09145"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559219.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84720.1"
                     /translation="MQKSEIIKTVNLTRIYGMGDVQVKALEDISLEILEGEFVAVMGP
                     SGSGKSTLMNLLGCLDRPTSGQYFLAGEDVSQLDKVQLAKIRNKQIGFVFQSYNLLPR
                     TSALENVMLPLVYDGNDQSTESEQRARAEAMLELVGLSDRMHHQPHELSGGQSQRVAI
                     ARALINDPVMLLADEPTGNLDSHSSYEIMDLLAGLNERGTTIVMVTHENDVAAYTRRV
                     IHFRDGKIQTDKLNGHRPLPSRVVEASHATL"
     gene            complement(5029..6648)
                     /locus_tag="VN363_09150"
     CDS             complement(5029..6648)
                     /locus_tag="VN363_09150"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="efflux RND transporter periplasmic adaptor
                     subunit"
                     /protein_id="HWQ84721.1"
                     /translation="MKHPFLIELVILAVLAAGCSSAASTPPASQADTPVFEQNQAQAG
                     LGSITLSATGAGSLVASNQTDLSFIEGGTLVELGVQEGDEVAAGSVLARLQVDLSAAG
                     LDEQRSAAELAVLLAQRAVDTLISSAELESAQALIRLETAQLALEEVEDDAPALAEAQ
                     QAVAEAKSAVEDAEMQVYISQSSASSDARYTAYAALLFKQQALADIEKQVSRLENQIK
                     SAGDKNSRDRLKHQMLELEVRLANHRLVVNVAQAKLDALDQPVDEDEAAQAQAQLAAA
                     TAQLADAERQLEQVTTTAGSSARSGAAALAQAELNEAQTAWEQLQNGPDPETLALAEA
                     ALKEAEAKLSQVKQTKLTLELVTPVSGTVLGINYTVGDWIAQRQTTLTVGDMAHPGVE
                     VYLAESDMSMAQVGAQAGVVFDVLPETTLSGVVTQVDPALITAGNAKTGRLVVELGDL
                     SALGSRWLPVGLNASVDVIGDTAENVVLVPIEALQEDSEGDYGVYLVQDEAVTLQPVT
                     IGLMDATRVEILTGLEAGQTVLLGEPTADGE"
     gene            complement(6668..8317)
                     /locus_tag="VN363_09155"
     CDS             complement(6668..8317)
                     /locus_tag="VN363_09155"
                     /inference="COORDINATES: protein motif:HMM:NF027890.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="HlyD family efflux transporter periplasmic
                     adaptor subunit"
                     /protein_id="HWQ84722.1"
                     /translation="MKSINMERTRVKGFEWKPFFQKNKFWLAGVLLVLLAAGGYGIYR
                     TNNAAQAAAETATTAELQTAVASTGSLTVLATGAGEVVTASEISLGFDESGTLLELLV
                     KAGDSVDAGQGLARLQTDRTAEYIALALAQAELNALTAQQELDNLNASWEMDAAEALL
                     AVEDAEQALEDLRNSNISQAEAAKAVTEAQTAVNQAQSIYSGARTTADADTIAAAKAE
                     LVLAQKQLNDIQEKFDDYVKKPDTDPEKANMQLKLSAAQGAYNTALRYYNAVTGTGSA
                     LDLESTAADLAAAQATLAQAQRTYERIKDGPSEGELAKANAELAVARAKYETLKTGVD
                     PLELSLTEATLANARASLAVAQEDRAVLELTAPQPGTILTVSAGVGEAVSTAAIITLA
                     DLNQPLLEVYLDEDDLEMAVVGYEAEVVFDSLPDNVFTGHVVEVSPVLYASMSADTIL
                     MKVELDVDSYAKPQSLPVGSNATVDVIGGRAENAVLVPVEALREIDDGEYAVFVMENG
                     EPRLRPVTVGLMDYTSAEITSGLEAGVTVTTGTVPTLQTSN"
BASE COUNT         1721 a         2575 c         2169 g         1934 t
ORIGIN      
        1 ggcgagatgt caaaaaaaat tctggttgtc gatgacgccg ctcaattacg caagctggtg
       61 aaaacttatc tggagcagga aggctatgcg gtggtgacgg ctgcagatgg gcaggaagct
      121 ctatacgtcg cccggcacga gaaaccagat ctgatcatcc tggatttgat gatgcccgaa
      181 atgggtggat acgagtttat gcgtgcttac accagggaag ccgacacacc cataatcctg
      241 ttaacagccc gggtcgagga aaacgacaaa gtgcttggcc tcgagctggg cgcggatgac
      301 ttcgtgacca aacccttctc gcctcgcgag ctggctgccc gcgtccgggc ggtcctgcgc
      361 cgaacagcca agcagaccgc gccgggcgac atcctgcgcg ctgcagatat caccctcgac
      421 cgtgctggtc gggtggtgct ggtcggcgag caccagattg atctgactcc ctccgagttc
      481 gatatcctgg ctgcactcct ggccgcgcca ggccgagctt ttacccgcct ggaactgctc
      541 gaacggcttc agggaaccgc ctatgaaggc tacgaacgga ccatcgacgt acatatccgc
      601 aatttgcgcg ctaaaatcga gcctgatgat acccaacccc ggtatgtgga aacagtctat
      661 gggatcggtt accggtttgc ttcggaacga cgataggtgt tgatcatgcg ctccctggct
      721 attaaactca ctctggcatt tcttatggtt ggcattgtag gcgctgtctc ggtggcgttc
      781 atggtgcgcg cccagactca acgccagttc agccagctgg ttatggataa aagccagcag
      841 accctggtta ccaacctgac ccaatatttt attgccaacc ggagctggtc cggagttgaa
      901 agtgttttcc accctgcgcc tgggaatttc gatccggccc cgaatccgat tgagcccttc
      961 gttgagcgac gcaccttatt tgccctggcg gacccaacgg gaaaagtcgt ttttggcgat
     1021 agccgaaatg atctggaagt cattaaatct gcggatctaa aacgtggtgt tggaataaag
     1081 gtggatgggg agaccgtcgg aacgctcatt tttgtgccat ctttaaatcg ctgggatcgg
     1141 ggcactcccg aggggaattt cctcaccgaa gtcagctttg ccagtctgcg cagtgccctg
     1201 attgccagcg tcctggctct cgggttgggc ggagttctgg ctttcacaat gacacgctcg
     1261 attcgcgacc tgaccacagc cactaccgtc cttgcgcagg gtaagctggg gcaccaggtt
     1321 aaagttcgct cccaggatga aattggagtt ctggcccact ccttcaataa aatgagctcg
     1381 gacctggagc atgccaacca gctccgccgg cagatgaccg ctgatattgc ccacgacctg
     1441 cgcacgccgt taagtgtcat cctgggctac accgaagccc tgagcgatgg aaaattgacc
     1501 ggatcgcccg agatatattc aatcatgcac acagagtcgc agcacctcgc gcacctgatt
     1561 gacgatctca aagtcctggc cctcgccgat gcgggggaga ttcccttgac cgtacaggca
     1621 gtgccgcctg ccagcttgct gcaccggaca gccgatgcct ataagattca ggctgagcaa
     1681 aaaggcatta ccatcgaagt tgagactgcc cctgctttgc cggaagtgat catcgatcac
     1741 gaaagaatgg cgcaagtaat gggaaatctg gtgagcaatg ccttgcgcta tacccccgcc
     1801 ggtggcagga tcagcctgtc cgccgctgag accggtggta aagtcagtat cctcatctcc
     1861 gataccggcg aaggcatctc tgccggagat ttaccttttg tattcagtcg ttcgtttcgc
     1921 ggggatcagg ctcgccagca agaaaacggt gaatcaggcc tgggactggc aatcgctaaa
     1981 tcgttggttg aagctcaggg cggggtaata tccgtcacca gccagccggg caaaggcagt
     2041 accttcagga ttgagttacc ggtgcttgaa aaataaaata atcaatagtg taaggcacag
     2101 atcacttgcc ggagcaaagc ctggctcaag tcccattact gatctacctg aatagtctcc
     2161 gccaggcaca ataagtaccc ctggaaagct tctcacaatg gtctccaggg gtactttttt
     2221 acaattttac cgtcagcttt atcctgcaga ttatcgatca ccgcatccag taattcggtt
     2281 gggatgctct gcattacgcc gccccggcgt acagcgctgg tcgaagcatc tgggtcaggc
     2341 atacctgccc cagccatgct cgggtccatg ccagccccag cggggttctc actcctttga
     2401 aagatgccgc cgcccggacc gcctccagca ggcattccac caccgcctga agttaatcca
     2461 ccacctcctc caggcatttc cggcaggtcg ccgctctccc tggcagcctg aagcgtctca
     2521 agcatctctg cggaaggagt ccccataggt ccaccacccc cgcccttcat ctcgatcccc
     2581 agcttttcgg atagcgacag catatcctcc cgggtcagct gcatggcggt gatcgcagcc
     2641 atctgttcag caggcattgt ctcctggatc tgtttaaaaa ttgcctggta ctcgtccgtt
     2701 gtgattgtgc tgctgcttcc caggctgcgg gcagccttcc acaaaggcag gagctccgct
     2761 gcagtttccg cgttgaccgg ctgttcgctg tcgtccagca gcaaagttcc cactgccagc
     2821 tttaattcgg tggacagccc tccctcaggg cctggatctt gcgtgccatc actcgcctgg
     2881 agggttgtgc cggtcgcggc ggcactgctg caggccgtaa gtaccaggct cagaaccaat
     2941 aaagacgtca acagactcga tcgtttcatt tcagaaattc ctccattatt ttttagcttc
     3001 atacgggaaa ttaaagggta ttgtctgaac cagacccatt aattggcaag ctgggttcct
     3061 gattattcat ggcgtaatgc ttggatagga tccagctggg cagctttatt ggccgggtaa
     3121 aaaccgaaga atacacccac tgcggctgca gatccaaatg ccagcaagat agacgatact
     3181 gtcaccagcg tgcgcatatc gcccagtttt ccaaataacc aggaaccgcc caccccggcc
     3241 aacacgccaa tcaggccgcc aaccaggctc aacaggagcg cttcggtcag gaactgccag
     3301 cggatattat tgggcgtcgc tcctaccgac tggcggatac cgatctcacg cgtgcgctcg
     3361 gtgacactga ccatcatgat attcataatt ccgatcccac ccacgaacag cgaaacgccg
     3421 gcaacccaac ccagcaagct ccggaaagct gctgtggtcg actcctgggt ttcgataatg
     3481 tcatcctgag agcggaccac gaaatccagg ctgtccaaag atacttcatg gtgttttgcc
     3541 agcagtacct ggataccata ggtaacctgg tcgattaagc tcttatcctc gacttctaca
     3601 tagattaccc gcacgctgtc tccgaacatc cgggcaaaca tatcgaaatt gaacttatcg
     3661 aataccactg tgatcggtgt gaacagatat gtgtcataat cgacattacc ggtgataccg
     3721 ctttccgcca tcacccctac cactgtcagt ttgacattgt tcacagtaac aatttgtccg
     3781 atggcggttg tttctccaaa cagctcctgt gccagggtcg cgccaagcac agctactttc
     3841 tgtttgcggt ccaggtcggt atctgtgaaa taacgcccca gcccgacatc aatatcccgc
     3901 accgatggga aatcggtcgt cgtgccaagg atagtgactt cttccaccgt gacactgccc
     3961 cccttaacat ctcccgaggc gctttgttct acaacgaccc cctgcacacc ggcgacattc
     4021 ttggcaatcg taaaagcgtc gtcgtaaacc agcccgccag tttgcggctg cccgggtccg
     4081 ccccgcatca tactcgcctg gacgtacagc aagttcgagc ccagggtggt gatctgctcc
     4141 tcgatggttt tctcggtccc ccggctgatc gagaccatga tgatcacagc tgccacgccg
     4201 ataatcaccc ccagcatggt caagagtgag cgcattttat tacggttcaa tccttgcgaa
     4261 gctatccgga tgactttaga gggtcgcatg gctcgcctct acaacccgag atgggagcgg
     4321 cctgtgtccg ttcagcttat ccgtctggat tttcccatcc cggaaatgga tcactcgccg
     4381 agtataagcc gcgacatcat tctcgtgggt gaccatcacg atcgtcgttc cgcgctcgtt
     4441 caatccggcc aacaggtcca taatttcata actggaatgc gagtcgaggt tcccggtcgg
     4501 ttcatccgcc agcagcatca cgggatcatt gatcaatgcc cgggcaattg ccacccgctg
     4561 ggattgaccg ccggacagct cgtggggttg gtggtgcatt ctgtctgaca ggccaaccag
     4621 ctcgagcatc gcttccgccc gggcgcgctg ttcactctct gtggattggt catttccgtc
     4681 atataccagc ggcagcatca cattctctaa agccgaggtg cgcggcagca gattgtaaga
     4741 ctggaataca aacccgatct gtttattccg gatcttggcc aactgaacct tgtcgagctg
     4801 gctgacatcc tctcccgcca ggaaatactg cccgctggtg ggacggtcca ggcacccaag
     4861 caaattcatc agcgtacttt tgcccgagcc cgagggtccc atgactgcca caaactctcc
     4921 ttcgaggatt tccagagaaa tatcctccag agctttcacc tggacatctc ccattccata
     4981 gatcctggtc agattaactg ttttgataat ttcagatttt tgcacaagtc actctccgtc
     5041 cgctgtaggc tcgcccagca gcacagtttg cccggcttcc aggccggtca ggatttcaac
     5101 tctggtcgcg tccatcaacc cgatggttac gggctgcagt gtcacagcct catcctgaac
     5161 gaggtagact ccataatctc cttcagaatc ttcctgtaac gcctcgatcg ggaccaggac
     5221 aacattctcc gccgtgtcgc cgatcacgtc tacactggca ttcaaaccca ccggcagcca
     5281 gcgggacccc agcgcgctca aatcacccag ctcaaccacc aggcgtccgg ttttcgcatt
     5341 ccccgcagtt ataagcgccg gatcgacctg ggtgaccaca ccactcaacg ttgtctccgg
     5401 taacacatcg aagacgacgc ctgcctgcgc cccgacctgg gccatgctca tgtccgattc
     5461 ggccagataa acttccaccc cggggtgggc catgtctccc actgtcaaag tggtctgtct
     5521 ttgagcgatc cagtccccta ccgtatagtt gatccccaaa actgttccgc tgaccggtgt
     5581 caccaactcg agcgtcaatt tggtttgctt cacttgagat aatttcgcct cggcttcttt
     5641 caacgctgct tcagccagag ccagtgtctc aggatcgggg ccattctgca gctgctccca
     5701 ggccgtttgg gcctcattca gctctgcctg cgccagtgct gcagcgccgc tccgggcact
     5761 gctccccgca gtcgttgtga cctgctcaag ctggcgctct gcgtctgcca gctgcgccgt
     5821 ggctgctgcc agctgggcct gcgcctgggc agcttcatct tcatcgaccg gctggtccag
     5881 ggcatccagc ttcgcctggg ctacattcac caccagccgg tggttggcca gccggacttc
     5941 cagctcgagc atttgatgtt tcaggcgatc gcgcgagttt ttatcaccag cgcttttgat
     6001 ctggttttcc agccggctga cctgcttttc gatatctgcc agcgcctgct gcttgaacag
     6061 cagggcagca taagccgtgt accgtgcgtc actgctggct gaagattgcg agatatacac
     6121 ctgcatctcc gcatcttcga ccgcagactt cgcttcagca acggcttgct gggcttctgc
     6181 cagagccggg gcatcatctt ctacctcttc cagagccagt tgggcggttt ccagccggat
     6241 caacgcctga gccgactcca gttcagcact gctgatcagg gtatccaccg cccgctgcgc
     6301 cagaagcacc gccagctctg ccgcgctgcg ttgttcatcc agcccggctg cgctcaaatc
     6361 tacctgcaag cgggccagga ctgacccggc tgcaacctcg tcgccctcct ggacacccag
     6421 ctccaccagc gttccgccct cgataaaact caaatcagtt tgattgctgg cgaccaggga
     6481 tcccgccccg gtggcgctta atgtgatgct gcccaggccg gcctgagcct ggttctgctc
     6541 aaataccggc gtgtcagcct ggctggctgg cggtgtgctg gcagcgctgc tgcagccagc
     6601 tgccaggaca gccagaataa ccagctcaat caggaatgga tgcttcacag ctgctcgcct
     6661 cccggggtta gttggatgtc tgcagcgtgg ggacggtgcc tgtggtgaca gttacaccgg
     6721 cttccagccc gcttgtaatt tcagccgagg tgtagtccat cagccctacc gtcaccggcc
     6781 gcaggcgcgg ctcaccgttt tccatcacaa atacggcata ctcgccgtca tcgatctcgc
     6841 gcaaggcttc tacgggaaca agcaccgcat tctctgcccg cccaccaatc acatccaccg
     6901 tggcattcga ccccaccggc agcgactggg gtttggcata cgaatccacg tccagctcaa
     6961 ctttcatcaa gatcgtatct gcgctcatcg aagcgtaaag caccgggctg acttctacca
     7021 cgtgcccggt aaagacgtta tctggcaggg agtcaaagac gacctctgcc tcatatccca
     7081 caacggccat ttctaaatca tcttcgtcaa ggtagacttc cagcagcggc tggttcaggt
     7141 cggccagcgt gatgattgca gccgtactca ccgcctcgcc caccccggcg ctgaccgtca
     7201 ggatggttcc gggctgagga gcagtgagct caaggactgc tctgtcctcc tgggcgactg
     7261 ccaggctggc tctggcgttt gccagggttg cttctgtcag ggacagctcc agcggatcga
     7321 ccccggtttt caaggtctca tatttcgccc gggctacggc cagctcggcg ttcgctttcg
     7381 ccagctcacc ttccgatggg ccatctttga tccgctcata cgttcgctgg gcctgggcca
     7441 gtgtcgcctg agctgcagcc agatccgcgg ctgtgctttc cagatccaga gcactgccag
     7501 ttcccgtcac agcattgtag tatctcaagg cagtgttata ggcgccttga gcagctgaca
     7561 gcttcagctg catattggcc ttttcgggat cagtatctgg cttttttacg taatcatcga
     7621 atttctcttg aatgtcattg agctgctttt gggctaagac cagctcagct ttggctgccg
     7681 caatcgtatc tgcgtccgcg gtggtgcgcg ccccgctgta gatactctgc gcctggttga
     7741 ctgctgtctg ggcttcggta actgctttcg ctgcctcagc ctggcttata ttgctgtttc
     7801 gcagatcctc cagagcctgt tctgcatctt ccaccgccag cagcgcctcc gcggcgtcca
     7861 tctcccacga ggcattcaga ttatccagct cctgctgcgc ggtgagcgca tttagctcag
     7921 cctgtgccag agccagggcg atatattccg cggtgcgatc ggtctgcaaa cgtgccagcc
     7981 cctggcctgc atctaccgaa tcaccggctt tgaccagcag ctccagcaaa gtcccgcttt
     8041 catcgaatcc caggctgatc tcggaggctg tcaccacttc gccggccccg gttgccagaa
     8101 ctgtcagcga gcctgtgctg gcgacagcag tttgcaattc ggcagttgtc gcggtctctg
     8161 ccgcggcctg ggcagcgttg tttgtacggt aaatcccata gcccccggcg gcgagtaaaa
     8221 ccaggagcac acctgccagc cagaatttat ttttttggaa aaacggtttc cattcgaagc
     8281 cttttacccg ggttctttcc atattgatcg atttcatacg ttgatctccc aaccagtttt
     8341 tcgtgaatta aggatcgcgg tttaaaagga aaaaatgcct gtacgcgttt gtagtatag
//
LOCUS       DATMXK010000501         3538 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_16462, whole genome shotgun sequence.
ACCESSION   DATMXK010000501 DATMXK010000000
VERSION     DATMXK010000501.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3538)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3538)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3538
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16462"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..871
                     /locus_tag="VN363_09160"
     CDS             <1..871
                     /locus_tag="VN363_09160"
                     /inference="COORDINATES: protein motif:HMM:NF013116.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="transaldolase family protein"
                     /protein_id="HWQ84723.1"
                     /translation="YKTIVRMGAEIYLPVYERSGHAYGHLSAQVNPYYFFDTNAMLSQ
                     ALELRALSPNIAIKIPGSYEGVQAIQELTALGVPTNCTSGYSVPQFIAVAEAVQAGIH
                     AARRTGVDLTGWRSVITYMSARWEAAPDFAAQASEAGVELSPEDVRWAGVAIFKNAYR
                     IFRQRAYPSKLLICSLRLGPRVDGAMRCWHMEETAGADVIFTLPPPFLTELFTQADHL
                     QFAPRIWEDIPAEVMARLRQVPYFNAAYDPDGMQPPDFNRLQCLLLTQKEFCSAMDKM
                     VAFTNQVLEEAPV"
     gene            1171..1431
                     /locus_tag="VN363_09165"
     CDS             1171..1431
                     /locus_tag="VN363_09165"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84724.1"
                     /translation="MLEIYTSQDYTGSLDLEFELRELGLEYKRIPTAPGEPAEIAIRN
                     GVRLVSGHNAVAAYIAELRYAVENWNWFASDSCYVDDSGENC"
     gene            complement(1434..1943)
                     /gene="dps"
                     /locus_tag="VN363_09170"
                     /gene_synonym="pexB"
     CDS             complement(1434..1943)
                     /gene="dps"
                     /locus_tag="VN363_09170"
                     /gene_synonym="pexB"
                     /inference="COORDINATES: protein motif:HMM:NF006975.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016722 - oxidoreductase activity, acting
                     on metal ions [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA starvation/stationary phase protection
                     protein Dps"
                     /protein_id="HWQ84725.1"
                     /translation="MAVKTNHQAGTKVKTSHDLPRDTREKMIRLLNTQLADTFDLFSQ
                     TKQAHWNVKGMQFYTLHELYDELAEGLLGYVDVIAERAVILGGAATGTVRMAASSSRL
                     DEFSAEPINSQGSIEELVKRYAALAATTRKGIDTADEAGDMDTSDLLIDVSRFLDKSL
                     WFLEAHIQL"
     gene            2699..>3538
                     /locus_tag="VN363_09175"
     CDS             2699..>3538
                     /locus_tag="VN363_09175"
                     /inference="COORDINATES: protein motif:HMM:NF012711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AMP-binding protein"
                     /protein_id="HWQ84726.1"
                     /translation="MEFQPIVKDPKSWSVQPNLLDYDTVYASFGWDQIHHELDGLPNG
                     GGINIAHEAVDRHANGPLRDKLALRWLGKSGEVKDFTFGDLKEQSNRFANLLHSFGVQ
                     KGDRVAVLAGRIPELYVAALGTLKNVSVFCPLFSAFGPEPIYQRLMRGEAKVLVTTKM
                     QYKKKIVEIWERLPALKAVLIADIDEDLQENVYSLPRLMAKASPEFTIPPTDPEDMSC
                     LHFTSGTTGMPKGAIHVHEAVYVHYMTAKYALDLHPDDIFWCTADPGWVTGTSYGVIA
                     PLLH"
BASE COUNT          768 a         1007 c          944 g          819 t
ORIGIN      
        1 gtataaaacg atcgtgcgta tgggtgcgga gatctacctg ccggtctacg agcgcagcgg
       61 gcacgcctac gggcatcttt ctgcccaggt gaacccgtac tatttcttcg acacgaacgc
      121 catgctctcc caggccctgg agctgcgcgc cctgagcccc aatatcgcga tcaaaatccc
      181 cggctcgtat gaaggcgtcc aggcgatcca ggagctgacc gccctggggg ttcccaccaa
      241 ctgcacctcc ggctattccg tgccgcagtt catcgctgtt gccgaagcag tccaggctgg
      301 aatccatgct gcccggcgga cgggcgtgga cctgaccggc tggcgctccg tgatcaccta
      361 tatgagcgcc cgttgggagg ctgccccgga ttttgctgct caggcaagtg aggctggtgt
      421 ggagctgagc ccggaggatg tacgctgggc aggcgtggcg atcttcaaga acgcctatcg
      481 cattttccgc cagcgggcgt accccagcaa gctgctgatc tgctccctgc gcctgggacc
      541 cagagtcgat ggggccatgc gctgctggca tatggaagag accgccggtg cggatgtgat
      601 cttcaccctg ccgcccccct tcctcaccga gctgttcacc caggcggatc acctccagtt
      661 tgcgccgcgc atctgggagg atatccccgc tgaggtaatg gcgcgtctgc gccaggtgcc
      721 ctatttcaat gcggcctacg acccggacgg gatgcagcca ccggatttca accgcctgca
      781 gtgcctgctg ctcacccaga aggagttttg ttccgccatg gataagatgg tcgcctttac
      841 caatcaggtg ctggaggaag cgccggttta aaaaaactat tcatgagctg accccacggg
      901 ttacaggtag agcattcgct aaggttcctc cccccatgag cagaccggac aggtttttca
      961 gtaatctgaa cggttctatt ccatgcgctg gatggtaatc tggacaccca ggtgtcatac
     1021 agaaacctgt ccggtccagt ccacgctagc gacgccgcgg gatggagatt tgatcgtata
     1081 gccgccatgc ggaaacttgt ccgatcgatt ggctggctct attgacgaaa acctgcttaa
     1141 accctacaat atagaccgga gaacacaggt ttgcttgaaa tttacacttc acaggactac
     1201 accggcagcc tcgatctgga atttgagctg cgcgagctgg gtctggaata caaaagaata
     1261 cccactgcac caggggaacc ggcggagata gccatccgca acggtgtgcg cctggtcagc
     1321 gggcacaatg ctgttgccgc ctacatagcc gaactgcgct acgcggtgga gaattggaac
     1381 tggtttgcca gcgattcgtg ctatgtagac gacagcgggg aaaattgttg agcttacagc
     1441 tggatatgcg cctccaggaa ccagagcgac ttatccagaa agcgcgaaac atcgatcaat
     1501 aaatctgatg tgtccatatc gccggcttca tcggcagtat cgatcccctt gcgggtggtt
     1561 gctgccaggg ctgcgtaacg ctttaccaat tcttcgatgc ttccctggct gttgattggt
     1621 tcagcactga actcatccag ccggctggaa ctggctgcca tgcgcactgt gccggtggcg
     1681 gcgccgccta aaatcactgc ccgttcggca atcacatcca cataccccag cagcccctcc
     1741 gccagctcat cgtacagctc gtgcagggtg tagaactgca tacctttaac gttccaatgc
     1801 gcctgtttgg tctggctgaa cagatcaaac gtatccgcca gctgggtatt aagtaagcgg
     1861 atcattttct cgcgcgtatc gcggggtaaa tcgtgggaag tcttcacctt ggtccccgcc
     1921 tggtgattgg ttttcacagc catttttaat cctcctggga atcttgaatt gagtatgttc
     1981 tttgcagggc gagtttctgg cacgccactg cagcttttgg ccaggtttta cctgacgtag
     2041 gcatatctgg ctaagctaaa cttagcttgg gtatacctag cttgactggg cttagcatag
     2101 cccgtctgag accacatgcc aatacgggat tcccctccat ctgcctcagg taaatcccta
     2161 cctgcgtttg ggctggcacc cgtttgtgtc aaatgtcact ataaagtcat gacataatac
     2221 taatttgtca tgtgatgact tatgctatta tgaaattgat cacaattgat acccatcctt
     2281 gattgatccg aggcggaagc tagtgcctgg aagcaggccg gggtgcacca cggtgtcatt
     2341 aatgccgcag accaagtgtc tgcggttatt tatttaacga ccctcaggac agaaaccgcc
     2401 cgcctcacac ctgttcagga aggaggctgg aaagactaaa tcacttaacg ttttttactc
     2461 atagaccggg atcaatcgct cctgcagtca tcaaccgatg cggcagcttc tgctaaacaa
     2521 aatcgatcac cttacaggat catgctcatc ctcccggagc tttattggat ggctggcgtc
     2581 tttctcccca ctcacagaaa tcttcaccgc aggctgcgcg gagagtctcg ggcgccggca
     2641 cttgttttaa tcgccctctg attatgctgg attggccttc aaataaaagg aggctccaat
     2701 ggagtttcaa ccgatcgtga aagatcccaa gagctggtct gtacagccaa acctgctgga
     2761 ttacgacacc gtgtatgcat cttttggctg ggaccagatc catcatgaac tggacggcct
     2821 ccccaacggc ggagggatca acattgcgca tgaagcggtt gaccggcatg ccaatggccc
     2881 tctgcgagat aagctggctt tgcgctggtt gggcaagtct ggggaggtga aggactttac
     2941 ctttggcgac ctgaaagaac aatccaaccg tttcgccaac cttctgcact ctttcggagt
     3001 tcagaaaggt gaccgagtcg ccgtcctggc aggtcgtatc cccgagctgt atgttgcagc
     3061 ccttggcaca ctgaaaaatg tcagtgtttt ctgcccgctg ttttctgctt ttggaccgga
     3121 accgatctac cagcgtttaa tgcgcgggga agcgaaggtc ctggtcacaa caaagatgca
     3181 gtataaaaag aaaattgtcg aaatctggga gcggctgcct gccttgaaag ctgtcttgat
     3241 cgccgatatc gatgaggatt tacaggaaaa cgtctattcc ctgcccaggc tgatggcaaa
     3301 ggcttcgccg gagttcacca ttccccccac tgatccggaa gatatgtcct gcctgcactt
     3361 caccagcggc accaccggta tgcccaaggg tgcgatccac gtccatgaag cagtctatgt
     3421 gcattatatg accgccaaat acgccctcga cctgcatccc gacgacatct tctggtgcac
     3481 tgccgatccc ggctgggtga ccgggacatc ctacggggtg atcgcccccc tgctgcac
//
LOCUS       DATMXK010000502         2903 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_38001, whole genome shotgun sequence.
ACCESSION   DATMXK010000502 DATMXK010000000
VERSION     DATMXK010000502.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2903)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2903)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2903
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_38001"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2903
                     /gene="rpoC"
                     /locus_tag="VN363_09180"
     CDS             <1..>2903
                     /gene="rpoC"
                     /locus_tag="VN363_09180"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559510.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000345 - cytosolic DNA-directed RNA
                     polymerase complex [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta'"
                     /protein_id="HWQ84727.1"
                     /translation="YRELKSRWGQVFRADMGAEAFYDILRRLDLDKLSEELWHEVRTT
                     RSKQKRKKATKRLKVVEAFRRSGNRPEWMILTVLPVIPPDLRPMVQLDGGRFATSDLN
                     DLYRRVINRNNRLKRLLELGAPDVIVRNEKRMLQEAVDSLIDNSQRGKALSRRGRREL
                     KSLSDMLKGKKGRFRRNLLGKRVDYSGRSVIVVGPKLKLYQCGLPKTMALELYRPFVI
                     SRLVHHQYAANVKGAKRFIERNRPEVWEVLEEVIKDRPVLLNRAPTLHRLGIQAFEPI
                     LIEGSAIQLHPLVTTAFNADFDGDQMAVHVPLSQKAVLEARQLMLSSRNLLKPADGEP
                     IISPSKDMVLGVYYLTKDDSQNRKGKGRVFGDADEAELAYSLDQVDIHAKITLRANTW
                     YDATNSRTPVAEERLIETTIGRVIFNRILPPAMQFVNWPLDKGGLKDLVAELYEVCGE
                     EDTPDIADAIKDIGFTYATRSGYSLAVSDITIPEQKNEIVNRALAEADTVQRDFRRGL
                     LTDQEQNERVIEIWQRTTNEVADAVKKAMDPNGNLAAQAISGATKGGFGPISQLAGMR
                     GLMADPSGRIIPLPIRSNFREGLNALEYFISTHGARKGLADTALRTADAGYLTRRLVD
                     VAQDVIINDDDCGTMEGLIISQDDDVAGQAIGSRLYGRLAAERVVDPLTGEILAERDD
                     PLDHERVRRITNANVHAIKVRSPLSCELQHGICARCYGLDLGRGAMVQIGSAVGTVAA
                     QSIGEPGTQLTLRTFHTGGVAAGGDITTGLPRVEELFEARRTPKGEAVVSELGGTVHI
                     MQSDRYSDLRLAHVVYSEMVSDEYDLPEGWTLAAEDGSEVAAGDVIAYLGEKTTIAAQ
                     NSGRARLENNKIIVSYEKRQEAEYEIPSTSRLLVKEGDQVTAGQPLTEGSLNPHLILR
                     IQGRTAAQLYLLSEVQKVYRSQGQNINDKHFEVITRKMLSKVQVTRPGDTNYL"
BASE COUNT          680 a          801 c          792 g          630 t
ORIGIN      
        1 ctacagggag ctgaaatccc gttgggggca ggtattccgc gctgacatgg gcgccgaggc
       61 tttctacgat attttacggc ggctggatct cgacaagcta tctgaagaat tgtggcatga
      121 agtacgcaca actcgtagca agcagaaacg gaagaaagcc accaaacgcc tgaaagtggt
      181 agaagcattc cggcgctcgg gcaaccgtcc cgaatggatg atccttaccg tcctgccagt
      241 aatcccacct gatctgcgcc caatggtcca gctggatggt ggacgcttcg ctacctccga
      301 tctaaatgat ctttatcgcc gggtgatcaa ccgtaataat cgcctcaagc ggctgcttga
      361 attgggcgct cctgatgtga ttgtgcgcaa cgaaaaacgg atgctgcagg aagctgtaga
      421 ctcgctgatc gacaactccc agcgtgggaa agctctttcc cgccgtggac gccgcgagct
      481 taaatccctc agcgacatgc tgaaaggtaa gaaaggccgc ttccgccgca acttgctcgg
      541 taaacgcgtg gattattccg ggcgttccgt gattgtggtc ggcccgaagc tgaaacttta
      601 ccagtgtggt ttgccaaaga caatggcgct ggagctttat cgcccatttg tgatttctcg
      661 attggtgcac caccaatatg cagccaatgt caaaggcgcc aagcgattta tcgagcgcaa
      721 tcgccccgag gtttgggaag tccttgagga agtgattaaa gatcgcccgg tcctgctcaa
      781 ccgtgcgcca accctgcacc gcctgggtat tcaggcattt gaaccgatcc tcatcgaagg
      841 cagcgctatt cagcttcatc cgctggtaac gaccgcattc aacgccgact tcgacggcga
      901 tcagatggct gtacatgtac ccctctccca gaaggcagtt ctggaagcac gccaattgat
      961 gctctcttct cgcaatcttc tcaagccagc agatggcgag ccaattatca gtccttccaa
     1021 ggatatggtc ctcggggtgt attacctgac gaaggatgac tctcagaacc gcaaaggtaa
     1081 agggcgagtt ttcggcgatg ctgatgaagc cgagctggct tacagcctgg accaggtgga
     1141 catccatgcg aagattacct tacgagccaa tacctggtac gatgccacca acagccgaac
     1201 tccagtcgca gaagagcgtt tgattgagac aaccatcggg agagtcatct ttaaccgcat
     1261 cctgcccccc gccatgcagt ttgtcaactg gccgctggat aaaggcggtt tgaaagatct
     1321 cgtcgccgag ctctatgaag tctgtggtga agaagataca ccagatattg ccgatgcaat
     1381 taaagacatt ggctttacct acgccacccg ttccgggtac tccctggcgg tatcggatat
     1441 caccatcccg gaacaaaaaa atgagatcgt taaccgggcc ctggccgaag ccgatacagt
     1501 acagcgtgac ttccgccgcg ggctgcttac cgatcaggaa cagaatgaac gtgttattga
     1561 aatctggcag cggactacaa atgaagtagc cgatgcggtt aagaaagcga tggatccaaa
     1621 tggcaacctg gctgcgcagg caatctctgg cgcaaccaag ggtggttttg gcccaatctc
     1681 gcagctggca ggcatgcgtg gcctcatggc tgacccgtct ggacgcatta ttcctctgcc
     1741 tattcgttcg aacttccgtg aaggcttgaa tgccctggag tacttcatct ctacccacgg
     1801 cgcccggaag ggtctggcag atactgcgct tcgaacagcg gatgctggct acctcacccg
     1861 ccgcctggtt gatgtggctc aagatgtgat tatcaacgac gatgactgcg gcacgatgga
     1921 aggattaatc atcagccagg atgatgatgt cgccggtcag gccatcggca gccgcttata
     1981 cggtcgtctg gcagcagaaa gggtcgtcga tcctctaact ggagaaatcc tggcagaacg
     2041 cgacgatccc ctggatcacg aacgtgtccg ccggataacc aatgccaacg ttcacgcgat
     2101 caaagttcgc tcgccacttt cttgcgagct tcagcatgga atttgtgcca ggtgttacgg
     2161 cctggatctt ggccggggag cgatggtgca gatcggatct gccgtaggta ccgtagcagc
     2221 tcagtccatc ggcgagccag gcacgcagct aaccctgcgc accttccata ccggcggtgt
     2281 tgctgccgga ggtgacatta ctaccggtct cccccgggtt gaagaacttt ttgaagcccg
     2341 ccgcacccct aagggtgaag cagttgtcag cgagcttggc ggaaccgtgc atatcatgca
     2401 gtccgaccgc tacagcgacc tgcgtctcgc acatgtggtt tacagcgaga tggtgagcga
     2461 tgagtatgat cttcccgaag gttggactct agcagccgag gatggctcag aggtcgcggc
     2521 cggggatgtg atcgcctatc ttggcgagaa aaccaccatc gcggctcaga attctgggcg
     2581 cgctcgtctg gagaacaata agatcatcgt ctcgtacgaa aagcgtcagg aagctgaata
     2641 tgagatcccc agcacttctc gcctgcttgt aaaagaaggt gatcaggtta ctgccggcca
     2701 acctctgacc gaaggctcat taaacccgca cctgatcttg cgcatccagg gacggactgc
     2761 tgcgcagctg tatctgctgt ctgaagtcca gaaagtttac cgttctcagg gccagaatat
     2821 caacgacaag cacttcgagg ttattacccg caagatgctc tcgaaggtcc aggtcactcg
     2881 tcctggcgat accaattatc tgc
//
LOCUS       DATMXK010000503         4081 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_76046, whole genome shotgun sequence.
ACCESSION   DATMXK010000503 DATMXK010000000
VERSION     DATMXK010000503.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4081)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4081)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4081
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76046"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            173..1432
                     /locus_tag="VN363_09185"
     CDS             173..1432
                     /locus_tag="VN363_09185"
                     /inference="COORDINATES: protein motif:HMM:NF013473.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ84728.1"
                     /translation="MLTRLSRTFHLYPFQFWMIFLGLLISTIGSSMIWPFLMVYVSEK
                     LDLPLSSVTILITLNSVMGLIFSLLAGPVIDKVGRKWIMAISLLVNGLAYIIQSQANS
                     LPVFAIAMALSGAFNPLYRVGADAMMADLIPGERRADAYSLLRTSNNLGVAMGPAIGG
                     MLVASSYSIAFYIAATGMILYSLLVTFRFRETLPSHTARRSLGATQKLSTRQLFGGYN
                     KVFGDRTYMTFIFFTILVMIPASMLWVLLSVYVKQNYAIPENKYGFLPTTNALMVVFL
                     QVAVTQITKRYRSLPVLAVGSLCYAIGVGSIAWMTGFWGFWISMVVMTIGELIQSPTA
                     STFAANRAPADMRGRYMSLYGLTWPLGSGIGPLLGGFLNDSIRPQAIWIGALVIGLLS
                     SSGFTLMALAERRRKPDTEPEVLAEQP"
     gene            1499..1813
                     /locus_tag="VN363_09190"
     CDS             1499..1813
                     /locus_tag="VN363_09190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84729.1"
                     /translation="MGLLQKLASIFSPQSKPGRYYEFTVRCHRCGEQITGRADLYNEV
                     SADFGEDGRGENYYVRKVLMGEGHCFQRIEVELFFDKNRHLVNKEIQGGLFVEESTPP
                     QE"
     gene            complement(1840..2250)
                     /gene="mce"
                     /locus_tag="VN363_09195"
     CDS             complement(1840..2250)
                     /gene="mce"
                     /locus_tag="VN363_09195"
                     /EC_number="5.1.99.1"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558701.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA epimerase"
                     /protein_id="HWQ84730.1"
                     /translation="MAKITKINHIAILVSDLESPLNFWRDALGLELTQVEEVPGEQAR
                     IAFLPIGDSEIELVQPTMDDSGLARYLEKRGPGMHHICLEVDDIDGMLAQLKEKGIQL
                     INEEAKMGSDGQRYAFIHPKSASGVMVELYELAK"
     gene            complement(2278..3933)
                     /locus_tag="VN363_09200"
     CDS             complement(2278..3933)
                     /locus_tag="VN363_09200"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558700.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylmalonyl-CoA mutase family protein"
                     /protein_id="HWQ84731.1"
                     /translation="MSHEDELQRWENETLAKTIKRFPERKTKFTTISGLPLPRLLIPP
                     DEDPDYAEKLGFPGEYPYTRGVQPSMYRGRLWTMRQYAGYATAEESNRRYRYLLEQGQ
                     TGLSVAFDLPTQIGYDADDPIALGEVGKVGVAISSLKDMATLFHEIPLDKVSTSMTIN
                     APAAILLAMYIAVAKRQGIEPRQLRGTIQNDILKEYIARGTYIFPPQPSMRLITDIFR
                     YCQAEIPHWNTISISGYHIREAGSTAVQEVAFTLANGIAYVQAAIDAGLDVDSFADQL
                     SFFFNAHNDFLEEVAKYRAARRLWAKIMRQRFQAKNSHSWVLRFHTQTAGSTLTAQQP
                     ENNVVRVALQALAAVMGGTQSLHTNSMDEALWLPTEKSVRVALRTQQIIAHESGVANS
                     IDPLAGSYLVEHLTDEIESQAQTYLERIDEMGGALAAINRGYMQAEIQDAAYRFQQAM
                     EQGEQVVVGVNAFQVEEKIELERLKVDPAIELGQRQRLADLRFQRDAARANEMLAQLE
                     NTARSGDNLMPVFIACVENDVTLGEICRVLRGLWGEYQPASWL"
BASE COUNT          814 a         1197 c         1111 g          959 t
ORIGIN      
        1 acctgaacaa aggcaaacga aaattatacc aagagtctcc gcccatactg attgcttcat
       61 ggaacgttaa cccagcattt gttgtagacc tgtacaggct aataattatt aaggtggttg
      121 cagtgattgt cttgtgaaca aggaataagg ggagtagaat ctgcaaccaa ccatgcttac
      181 acgcctcagt cgcacttttc atttataccc ctttcagttc tggatgatct ttttgggctt
      241 gctgatcagc accatcggct ccagtatgat ctggcctttc ctgatggtgt atgtcagcga
      301 gaaacttgac ttacccctga gcagcgttac catcctgatc accttgaatt cggtaatggg
      361 gttgatcttc tccttgcttg ccgggccggt gatcgacaaa gtcgggcgta agtggatcat
      421 ggcgatcagc ctgctggtga atggactggc ttatatcatc cagagccagg ccaactctct
      481 gccagtcttc gccattgcca tggcgctcag cggagcattc aacccgctct accgggtagg
      541 cgccgacgcc atgatggccg acctgatccc cggtgagcgc cgcgccgatg cttattcctt
      601 attgcgtacc agcaataacc tgggcgtggc catgggtcct gccatcggtg gaatgctggt
      661 ggcttcttca tattccatcg ccttctacat cgctgccacc ggtatgatcc tgtatagctt
      721 gctggtgacc ttccgctttc gggagaccct ccccagccac acagcaaggc gctcgctcgg
      781 agccactcag aagctctcta cccgccagct gttcggcgga tacaacaagg tctttggcga
      841 ccgtacctat atgaccttta ttttcttcac catcctggtg atgatcccgg cgtctatgct
      901 ttgggtcctg ctttcggtgt atgtcaagca gaactacgcc attcccgaaa acaagtatgg
      961 attcttaccc accaccaacg ccttgatggt ggttttcctg caggtagccg tcacccagat
     1021 taccaagcgc taccgctcgc tgcccgtcct ggcagtcggc agcctgtgct atgccatcgg
     1081 cgtggggagt atcgcctgga tgaccggctt ttggggcttc tggatcagta tggtggtcat
     1141 gaccatcggc gagctgattc agtcacccac ggcctccaca tttgccgcca accgcgctcc
     1201 ggcagacatg cgtgggcgct atatgagcct gtacggcctg acctggcccc tgggatcggg
     1261 gattggcccg ctgctgggcg gctttctcaa tgacagtatc cgcccccagg ccatctggat
     1321 tggcgccctg gtgattggcc tgctcagctc aagcggcttt accctgatgg cactggccga
     1381 gcgccggcgc aagccagata ccgagcccga ggttttagcc gaacagccct gatctgagcc
     1441 tataattggg gctgagcgtc taaacctcaa tcccaggacg atcagaagga tactagcaat
     1501 gggactcttg caaaaactgg ccagcatatt cagcccgcaa tccaagccgg gtcgttatta
     1561 cgaattcacc gtgcgctgcc accgctgcgg cgagcaaatc accgggcggg cagacctgta
     1621 taacgaagtc agcgcggatt ttggcgagga tggcagggga gaaaattact acgtgcgcaa
     1681 ggtgctgatg ggggaaggac attgtttcca gcggattgaa gtcgagctgt tcttcgataa
     1741 aaaccgccac ctggtcaata aagaaatcca gggcgggttg tttgtcgaag aatcaacacc
     1801 gccccaggaa taaacttacg ttctctgtac tatccaggct tatttcgcca gctcgtacaa
     1861 ctctaccatt actccgctgg cgctcttggg gtggataaat gcgtagcgct gcccatcgct
     1921 tcccatcttt gcttcctcgt tgatcaactg gatccccttt tccttgagct gcgccagcat
     1981 gccgtcgata tcatccactt ccaggcagat atggtgcatc ccagggccgc gcttttccag
     2041 atagcgcgcc agccccgaat cgtccatcgt cggttggacc agctcgatct cgctgtctcc
     2101 gataggaaga aatgcaatcc gcgcctgctc accagggact tcctccacct gggtcagttc
     2161 caatcccagg gcatcccgcc agaaattcaa ggggctttcc aggtcgctga ccaggatcgc
     2221 aatatgattg attttggtga tcttagccac aggaatacat ccttcttgtt atatatttta
     2281 cagccaggaa gccggctggt actcgcccca cagcccgcgc agcacccggc agatctcgcc
     2341 cagggtgacg tcattctcca cacaggcgat aaacaccggc ataagattgt cgccactgcg
     2401 agctgtgttt tccagctgag ccagcatctc gttggcgcgc gccgcatccc gctggaagcg
     2461 cagatcggcc agtcgctggc gctgccccaa ctcgatcgcc ggatcgactt tcaggcgttc
     2521 cagctcgatt ttttcttcca cctggaaggc attcaccccc acgaccacct gctcgccctg
     2581 ctccatcgcc tgttgaaagc gataagcggc atcctggatc tcggcctgca tgtagccgcg
     2641 attgatcgct gccagcgccc cgcccatctc gtcgatccgt tctaggtagg tctgtgcctg
     2701 gctttcgatc tcatcggtaa ggtgctctac caggtacgag cccgccagtg gatcgatcga
     2761 attggccacc cccgattcat gagcgatgat ctgctgggtg cgcagggcca cccgcaccga
     2821 tttttcggtt ggcagccaca gggcttcgtc catgctgttg gtgtgcagcg actgcgtccc
     2881 acccattacc gccgccagag cctgcagtgc cacccgcacc acgttgttct ccggctgctg
     2941 ggcggtcagg gtcgagccag cggtctgggt atggaagcgc agcacccacg agtgcgagtt
     3001 tttggcctgg aagcgctggc gcatgatctt cgcccacagg cggcgcgcgg cgcggtattt
     3061 agcaacttcc tccagaaaat cattgtgggc gttgaaaaag aatgaaagct ggtcggcaaa
     3121 gctgtccaca tccagcccgg cgtcaatcgc tgcctggacg taagcgatac cgttcgccag
     3181 ggtgaaggcc acttcctgca ccgccgtcga gccggcttcg cggatatggt agcccgaaat
     3241 gctgatcgta ttccaatggg ggatttcggc ctggcagtaa cggaagatat ccgtgatcag
     3301 gcgcatggat ggctgcggcg ggaagatata cgtgccgcgg gcgatatatt ctttcaggat
     3361 atcattctgg atcgtaccgc gcagctgcct gggttctatc ccctggcgct tcgccacggc
     3421 aatatacatc gccagcagga tggctgccgg ggcgttgata gtcatactgg tggagacttt
     3481 gtccagcggg atctcgtgga acagggttgc catatctttc agcgaggaga tcgccacacc
     3541 taccttgccc acctcaccca gggcgatggg atcgtccgca tcataaccga tctgggtcgg
     3601 cagatcgaaa gccaccgaaa gcccggtctg gccctgctcc agcaggtagc ggtagcggcg
     3661 gttggattcc tccgccgtgg catagccggc gtactggcgc atggtccaca gccgcccgcg
     3721 gtacatgctg ggctgcacgc cgcgcgtgta ggggtattcg ccggggaagc ccagcttctc
     3781 agcgtaatcg gggtcctcat ccggcgggat cagcaggcgc ggcaggggca gacctgaaat
     3841 agttgtaaat ttagttttgc gctccggaaa gcgcttgatg gttttcgcca gggtttcgtt
     3901 ttcccagcgc tgtagttcat cttcatgcga catggtatta acctcaaggt agactgattt
     3961 tcagtatacc ccggggaaag ttgatttgcc agagagcttt gtaccgcctt tggcgtgatt
     4021 tattgcctgg actggagaat tcatgaattc cttggttata attattctcc actacatatt
     4081 c
//
LOCUS       DATMXK010000504         2830 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_141663, whole genome shotgun sequence.
ACCESSION   DATMXK010000504 DATMXK010000000
VERSION     DATMXK010000504.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2830)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2830)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2830
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141663"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..908)
                     /locus_tag="VN363_09205"
     CDS             complement(<1..908)
                     /locus_tag="VN363_09205"
                     /EC_number="2.7.1.11"
                     /inference="COORDINATES: protein motif:HMM:NF012585.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="6-phosphofructokinase"
                     /protein_id="HWQ84732.1"
                     /translation="MEGSSEFGRIGILVGGGPAPGINGVIHSVTNQAYQHGIEVFGIL
                     DGFKHLMEGKVVGEILTPKSVAFIHHEGGSILHTARANPTTSTRAMENCVQALSSAGI
                     TCLVSIGGDDTAFSASRVAQYAQDVMGISLRSVHIPKTIDNDLPLPEDIPTFGFETAR
                     DLGAQLVMNLKRDALTSQHWFLVLTMGRKAGHLALGIGNSSAATITLIPEEWTGREIR
                     LQEIVDILTCSILLRFLEGKPYGVALLAEGILENLSHEDLNCLDNVERDEHGHIRLAE
                     VNFLDILKKELEGELDRLGISVRLVK"
     gene            1045..1302
                     /locus_tag="VN363_09210"
     CDS             1045..1302
                     /locus_tag="VN363_09210"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84733.1"
                     /translation="MPRLIPAAERITRARALIQKARKLPLPPDGNKFDFTYIAKVKDI
                     LRQARDLIKFIPDTPSATDEIKAEVKQIFAELEQANREILH"
     gene            complement(1408..2715)
                     /locus_tag="VN363_09215"
     CDS             complement(1408..2715)
                     /locus_tag="VN363_09215"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84734.1"
                     /translation="MKITGNTLWKNGFTVVLALSLAALACQFPGAGSPTAEVLQTNTP
                     DIGPEETEVVSPSDTPEIVLPTDTPDVTFQIQRQAARVLFGAADPLGELTTNDAQPFQ
                     EGSLITTDPQGEALISGELEGEQCSIFVFFNSQLRKSACPESALTSGNASCLESGSAV
                     FQNCANHLVMTPSGNAQLLGTWVQVVYLPEQRMSAYVVADGRVQVQPIDQQGLAGNWT
                     TVRPGEFYYTSPDMAESAVPTLPRRQVLSWDRLPALVQFYNLTDYIGRGTERAVRSGF
                     AYRDAETLLGPADLIVEVELANPSERGFPLDWYNRLSDNYYKIPIRMRITNRGGKETG
                     EFKLSAEGGTPDNWFVRPLAGEDQPQEGFYAYVREGILPGGELNMYGMIVITDTLLAT
                     QPLSFIITVDSCAGDEIFTKYCRVDESDEGNNLSTQIDVPPRG"
BASE COUNT          681 a          789 c          702 g          658 t
ORIGIN      
        1 tatttcacca ggcgaacgct gatccctaaa cgatccagct cgccttccaa ttcctttttt
       61 agaatatcaa ggaaatttac ttccgccaga cgaatgtgac cgtgttcgtc gcgttccaca
      121 ttgtcgaggc agttcaaatc ctcgtgggat aaattctcca gaattccttc ggccagcagc
      181 gccacaccat aaggcttgcc ctccaggaaa cggagtaaga ttgagcaagt caggatgtct
      241 acaatctcct gtaagcggat ttctctcccg gtccattcct cagggatcaa agtaatcgtc
      301 gctgcactgc tgttgccgat ccccagagca aggtgacctg ccttgcgtcc cattgtcagc
      361 accaggaacc aatgctggct ggtcagggca tcccgtttca gattcatcac cagctgcgcc
      421 ccaagatcgc gggcggtttc aaatccaaaa gtcgggatat cttcgggcag cggcaggtca
      481 ttatcgattg tttttgggat atgcactgag cgcaagctga tccccatcac atcctgcgca
      541 tattgggcaa cacgggaggc agaaaaggca gtgtcatcgc cgccaatgga aaccaggcag
      601 gtgatccccg cactggacaa ggcctggacg cagttttcca ttgcccgggt agacgttgtg
      661 gggtttgccc gcgcggtgtg aagtatagag ccaccttcgt gatggataaa tgcgacggat
      721 ttcggcgtca gaatctcccc caccactttg ccttccatga gatgtttgaa accgtccagg
      781 atcccaaaca cctcgatgcc gtgctggtac gcctgatttg tgaccgaatg aatgacccca
      841 ttaatccccg gcgcagggcc tccacctacc aggatcccaa tcctaccaaa ttcactgcta
      901 ccttccattt tttttgacct ccgccgggct tgacgagttt ctttccttga acgattatat
      961 atcacatcct gactcgtgaa acgcagatag aattatttgg aaggaaagag aagtagtaga
     1021 atttatccac gcaaaggagc gcatatgcca cgcctgatcc cagctgcaga acgaatcaca
     1081 cgcgcccgcg ctttgatcca aaaagcgcgc aaactgcccc tccccccaga tggcaataaa
     1141 ttcgatttta cttacattgc taaggtgaag gatattctgc gccaggcgcg cgatctgatc
     1201 aagttcatcc cggatactcc cagtgcaacc gatgagatta aggcagaggt caaacagatc
     1261 ttcgctgaac tcgaacaggc aaaccgggag attctacatt gatatgagga tttatccaac
     1321 agtcgagcta ataaatacct gatttggaag accagatcac caggctccgg gattgggctg
     1381 ccggatgccc aggtctgaat tcttgattta tcctcttggt ggaacgtcga tctgcgtaga
     1441 caggttgttc ccctcatcag attcatctac acggcagtat ttggtaaaga tctcatctcc
     1501 ggcgcagctg tcgaccgtga ttataaaact caacggttgg gtcgccagca aggtgtcggt
     1561 gataacaatc attccataca tatttaattc acctcccggc aggattcctt cccttacata
     1621 agcataaaac ccttcctggg gttggtcttc acccgccaaa ggccttacaa accaattatc
     1681 gggagtacca ccttcggcag ataatttaaa ttctccggtt tctttccctc cgcgattcgt
     1741 gatgcgcatg cggatgggga ttttatagta attatctgac aggcggttgt accagtcgag
     1801 aggaaaacct cgctctgaag gattggccag ctcaacctcc acgattaaat ccgcgggccc
     1861 aagcagtgtt tctgcatcgc gataggcaaa gccgctcctg accgcacgct cggttccacg
     1921 cccaatataa tcggtgagat tgtagaattg gaccagggcg ggcaggcggt cccaggataa
     1981 tacctgccgg cgcggcaggg tggggacagc cgactcagcc atatctgggg acgtgtaata
     2041 gaactcacct ggccggacag ttgtccaatt cccagccagt ccttgctggt cgatcggttg
     2101 gacctgcacg cggccatccg ccacaacata tgcactcatc ctttgttccg gtaaataaac
     2161 gacctgaacc caggtaccca acaattgggc attccctgat ggggtcatta ccagatggtt
     2221 ggcgcagttc tggaaaacag ccgatcccga ttcaagacag ctggcgtttc cactggtcag
     2281 ggctgactcc gggcaagcac tcttgcgaag ctgagagttg aaaaaaacga agatgctgca
     2341 ctgctccccc tccagctcac ccgaaattag agcctccccc tgtggatccg tcgtgatcag
     2401 actgccttcc tggaaaggct gggcgtcatt ggtcgtcagc tcgccaagcg gatcggccgc
     2461 accgaaaagc accctggctg cctggcgttg gatctgaaat gtgacatcgg gtgtatcagt
     2521 gggaaggaca atttccgggg tgtccgacgg gctcacaacc tcagtttcct cggggccgat
     2581 atccggtgtg ttggtctgga ggacttccgc tgtgggtgaa ccagctccgg gaaactggca
     2641 ggctaaagct gccaggctta atgccagaac aactgtgaat ccattcttcc aaagggtatt
     2701 acccgtaatt ttcatcagct ttactcctgc ctgcactcca tgactggcgg gtgcagattt
     2761 agctcacatc caggcgagaa tacaggcgcc tggcaagctg cagcccgaat agaacacccc
     2821 aggctaatac
//
LOCUS       DATMXK010000505         3026 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_1840, whole genome shotgun sequence.
ACCESSION   DATMXK010000505 DATMXK010000000
VERSION     DATMXK010000505.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3026)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3026)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3026
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1840"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1867
                     /locus_tag="VN363_09220"
     CDS             <1..1867
                     /locus_tag="VN363_09220"
                     /inference="COORDINATES: protein motif:HMM:TIGR01525.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042625 - ATPase-coupled ion transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="heavy metal translocating P-type ATPase"
                     /protein_id="HWQ84735.1"
                     /translation="SLVMSLVAGVLLFAGWLLETSGGSDPRLSLGLYLAAVGIGGWEI
                     AREAWHELRQRRLSTDSLMILAALGAVALGEYAEGSLLIFLFSLGHALEERLIARARS
                     AIDSLADLTSPEAVVIRNNQPVEVLVTEIGLGETVLIRPGTRFPIDGVVLEGQSSVDE
                     SPVTGESLPVPKTAGDKVFAATLNGSGALQVRATRLAEDSTLARVIKMVREAQSAQSP
                     VSKSVTRFMDRFVPVVVAITLLLVIVPPLFGVPFKESFIRAMIFLVAASPCALAVGPS
                     SAVIAGISQAARKGVLIKGGVFLEKLGRMTTVAYDKTGTLTDGTFKLERVIPLNGIPE
                     QIILSLAATVEAQSGHPLARAVRGAAETAGLELPVAEEAEEWTGRGVQARVAGQVVQV
                     GSADWIAQQDGGIDHQTQATLHDLKEQGYSVLVVRQMDQLQGILAFSSEARPEAQEAI
                     ASLRRLGIQQQIMLSGDQPQAARRLAGILGLDGWKANLHPEDKLEQIKSLQAEYGSVA
                     MVGDGINDAPALAQADIGIAVGGAATDVALQTADVALMGANLSNLVTAVNISRKTQSL
                     VRQNLILALGVMLVLSVAALSGWVNVGPAVILHETSTVLVVLNSLRILGTGAPKF"
     gene            1967..2326
                     /locus_tag="VN363_09225"
     CDS             1967..2326
                     /locus_tag="VN363_09225"
                     /inference="COORDINATES: protein motif:HMM:NF012790.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="rhodanese-like domain-containing protein"
                     /protein_id="HWQ84736.1"
                     /translation="MNKNQKPRSRSVPLLLMVVGLVLVIIAIFFSLNLPKTAPVPDAS
                     AQPGTAAIPHPDVPRVSLADARAAYDIGSAVFVDVRGEPYFTQGHIQGALSVNEADLD
                     AGIAALDPQAWIITYCT"
     gene            2483..2983
                     /locus_tag="VN363_09230"
     CDS             2483..2983
                     /locus_tag="VN363_09230"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DinB family protein"
                     /protein_id="HWQ84737.1"
                     /translation="MPDSAVHLAERLRVEGEKSLAFFASLPEQAWEILIYTDGSVWKL
                     HQIIAHYASAEANFGLLIDQILAGGSGAAEDFDLNRFNRSQVGRLETLPRQELLARYK
                     ISRQDNIARVERMQPADLDRQGRHPYLGLTSLSEIIKMIYRHNQIHQREIRQILTAAS
                     PNQLPA"
BASE COUNT          617 a          864 c          911 g          634 t
ORIGIN      
        1 cagcctggtt atgagcctgg tcgcaggtgt gctgctgttt gccggctggc tgctggagac
       61 cagcggcgga tctgacccga ggctcagtct cggattatac ctggcggcag tcgggattgg
      121 cggttgggag atcgcccggg aagcctggca tgagctgcgt cagcggcgct tgagcacaga
      181 tagcctgatg atcctggctg ccctgggagc cgttgccctg ggcgaatatg ctgaagggtc
      241 cttattgatc ttcctcttca gcctggggca tgccctggaa gagcgcttga tcgcccgcgc
      301 ccgttctgcc atcgattcgc tggctgatct cacttcacca gaggctgttg tgatccgcaa
      361 caaccagccg gtcgaagtcc tggtcaccga gatcggcctg ggcgagacgg ttttgattcg
      421 ccccgggacg cgtttcccca tcgatggggt tgtgctggaa ggccagtcct ccgtcgatga
      481 atcaccggtc accggagaaa gtctgcccgt cccaaaaaca gccggagata aggtctttgc
      541 tgccacgttg aatggcagtg gagccctgca ggtgcgcgcc acccgtctgg cagaagacag
      601 caccctggcg cgtgtgatca agatggtgcg cgaagcccaa tccgcccaat cgccggtgtc
      661 taagtctgtc acccgcttca tggaccggtt tgtaccggtc gtggtagcga ttaccctcct
      721 gctggtcatt gttccaccgc tgttcggtgt gccatttaaa gaatcattta tccgcgccat
      781 gatcttcctg gtggctgcct caccctgcgc cctggcggtc ggacccagct cggcggtcat
      841 cgccgggatc tctcaggcag cccgcaaagg tgtgctgatc aaagggggtg tattcctgga
      901 aaaactgggc cgcatgacga cggtggcata cgataagacc ggcactctga cagatgggac
      961 attcaagctt gagcgtgtaa ttccattaaa tgggatcccg gagcagatta ttttaagcct
     1021 ggcggcaact gttgaagctc aatcaggcca tcctctggcg agagctgtac gcggggctgc
     1081 cgagacagcc gggctggagc tgccggttgc cgaagaagcc gaagaatgga ccggtcgcgg
     1141 cgtgcaggcg cgcgttgccg gtcaggttgt ccaggtaggc agcgccgact ggattgcgca
     1201 gcaagatggt ggcatcgacc accaaaccca ggcgaccctc cacgatctta aggagcaggg
     1261 ttattcggtg ttggtcgtcc gccagatgga ccagctccag ggtatcctgg cgttcagcag
     1321 cgaagcccgc cccgaagctc aggaagctat tgccagttta cgcaggctgg gtatccagca
     1381 gcagatcatg ctttcaggcg accagcccca ggctgctcgc cgcctggctg gtatcctggg
     1441 tttggacggc tggaaagcca acctccaccc ggaagacaag cttgaacaga tcaaatccct
     1501 gcaagcggaa tatggcagcg tggccatggt cggggatggg atcaatgacg ccccggcgct
     1561 ggcgcaggcg gatatcggga tcgcggttgg tggcgctgcc acagatgtcg cgctgcagac
     1621 tgcagacgtg gcgttgatgg gcgccaacct gtcgaacctg gtcactgcag tcaatatcag
     1681 ccgcaaaacc cagagcctgg tcaggcaaaa tcttattctc gccctcgggg tgatgctggt
     1741 gctcagtgtt gcagctctga gcggctgggt gaatgtcggt ccggcagtta tcctgcacga
     1801 gaccagcacc gtcctggttg tattgaattc tctgcgcatc ctcggaactg gcgcgcctaa
     1861 attctaagtc atctccagga taagaatctg cgaagaatgc ggtctgacat tccgccgcgt
     1921 gaataggtta tggtatcatc atcgcgccga ggaggaccaa cactggatga ataagaacca
     1981 aaaaccgcga tctcgatctg tccctttact actaatggtc gttgggttgg ttctggtcat
     2041 catcgcaatc ttcttcagcc tgaacctacc caagacagca cctgtgccgg atgcaagcgc
     2101 gcagcctggc acagccgcca tcccacaccc ggatgtgccg cgggtcagcc tggcagatgc
     2161 cagagccgct tatgacatcg gcagcgcggt cttcgtggat gtgcgcggtg agccatactt
     2221 cacccaggga catattcagg gagcgttgtc ggtcaacgaa gccgacctgg acgcaggcat
     2281 cgccgccctc gatcctcagg cctggatcat cacctactgt acctgacccg ccgaagaatc
     2341 gagcgcccgt gtggcgtcgc tgttgatcga acgtggatat tcgaacgtga tggctctgaa
     2401 gggtggtctg gaagcctggt cggcagctgg ctatccgatg gagtaagtac ctgaattagc
     2461 atggcttgat cgaggagcaa agatgcctga ttccgcggtc cacctggccg agcgcttgcg
     2521 ggtagagggt gaaaaatcgc ttgctttttt tgccagcctc cccgagcagg cgtgggaaat
     2581 cctgatttac accgatggca gcgtctggaa gctgcaccag atcatcgctc attatgcctc
     2641 cgcagaagcc aactttggct tgctgatcga tcaaattctg gcagggggaa gtggagcagc
     2701 cgaagatttc gacctgaatc gcttcaatcg cagtcaggtt ggccgtcttg aaaccctgcc
     2761 gcgccaggag ctgctggcac gatacaaaat cagtcgccag gataatattg cccgggtgga
     2821 gcgcatgcag ccagcggatc tggatcgcca gggacgccat ccttatctgg gccttacgtc
     2881 gctgagcgag attatcaaaa tgatctaccg ccacaaccag attcaccagc gtgaaatccg
     2941 ccagattctg accgctgcca gtcccaacca actgcctgct taatcaggcg ggtgaccttg
     3001 ctgtcgttca aaacaaagcc atggaa
//
LOCUS       DATMXK010000506         7057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_16340, whole genome shotgun sequence.
ACCESSION   DATMXK010000506 DATMXK010000000
VERSION     DATMXK010000506.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16340"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            61..453
                     /locus_tag="VN363_09235"
     CDS             61..453
                     /locus_tag="VN363_09235"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84738.1"
                     /translation="MINEVVLEGIVVKTWKYAEDLLFRIACYRDPDLPSKSLNDLQDA
                     ADFVTIRVLKGNLGAPVVVEKGVHVRVHGFLQSRDYEESLADFLKDARGAELPVPEGL
                     DPNELRAPRGTTEVIARRIMSQRNGSKR"
     gene            1036..2181
                     /locus_tag="VN363_09240"
     CDS             1036..2181
                     /locus_tag="VN363_09240"
                     /inference="COORDINATES: protein motif:HMM:NF016024.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Rad52/Rad22 family DNA repair protein"
                     /protein_id="HWQ84739.1"
                     /translation="MTIAPPILENQLQHDRMVRAQAALMNGDMQVMVLTDCTWMVASK
                     SSRYTVSLDGDAWTCTCLDFTGRCQRFGLRCKHIEAVRLTETEKIAEPRLVNQYTYPQ
                     FSTTYTEEQMTQSMPTHDPPTLDPSSEQILWRLKQPLDMNRVKRRQAPGQGTVPYLEG
                     YDVIEAANDLFLFRWSFDLLSEPHIMRWDKVITFYDQRARKKVPVLGEDGKPTTEIAG
                     IAYITGRITVELDGKAFSHADAGRCIFTGDTPEALDMAIAGAVTDCLKRCFRQLGEQF
                     GNSLYDKEVAKTAGLENGASNGHGSNGNGQSEKSTGQTTPPNPTVPPNPPASQAEALQ
                     YRDGVAVDTSNAAELEAFNAFKSAHQELAPASREVLRAWVASRNGKK"
     gene            2212..2631
                     /gene="ssb"
                     /locus_tag="VN363_09245"
     CDS             2212..2631
                     /gene="ssb"
                     /locus_tag="VN363_09245"
                     /inference="COORDINATES: protein motif:HMM:TIGR00621.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003697 - single-stranded DNA binding
                     [Evidence IEA];
                     GO_process: GO:0006260 - DNA replication [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="single-stranded DNA-binding protein"
                     /protein_id="HWQ84740.1"
                     /translation="MSFQTIIIAGHLGRDPEMRYTPAGQAVTNFNLAANRQYTDSSGQ
                     VVKETTWFRVSAWGKTAENCNQYLHKGSLVLVEGRLVCDPETGGPRLYTRQDGSPASS
                     FEVSASTVRFLSGREEEGEQEPAGNGSAASQHDDIPF"
     gene            2697..3044
                     /locus_tag="VN363_09250"
     CDS             2697..3044
                     /locus_tag="VN363_09250"
                     /inference="COORDINATES: protein motif:HMM:NF040013.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF6166 domain-containing protein"
                     /protein_id="HWQ84741.1"
                     /translation="MIKKIYRGEPGGAVKVTLPSGKARPLRHVVLHSPTGFGWGYGGS
                     GPADLALSILCDVLSERPTEKQIYHGRFIAYPHHQDFKREFVARWEYGSGFEIDSETI
                     SDWLRKRGVEVQP"
     gene            3041..3310
                     /locus_tag="VN363_09255"
     CDS             3041..3310
                     /locus_tag="VN363_09255"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84742.1"
                     /translation="MTDEYISDDGPGSDLFDPYETGWWKSISSDDSAPADQPCPNCNG
                     EGYVVVWVYPWDGCSYDEEPCKFCNPRKDRSELDDEPIKEMDIPF"
     gene            3363..3788
                     /locus_tag="VN363_09260"
     CDS             3363..3788
                     /locus_tag="VN363_09260"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84743.1"
                     /translation="MQDTLTQTPKVDLTGRPTRIRVIGYTYLVDFGPSTQPRFHTVNK
                     QRSCSCPLKETCPAIDAVAEYLRNGGQRAPDPMPPCPICGAETVRDRKWDGKYTKELG
                     WRCTAGGLRHFLEAKAERIKEALRRNQAAVSEHESAAGR"
     gene            3806..4270
                     /locus_tag="VN363_09265"
     CDS             3806..4270
                     /locus_tag="VN363_09265"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84744.1"
                     /translation="MMQVHTLTHMEFEPAPVQVGEPFRCWECGSPDQGGMFLIIDHSP
                     LEAYTDHVYYMRLSCGHNNRCVRLGDAVAPLRPLDRSIDENLASVREGLAHLAKAVPP
                     DQQNQEPVSKIVYLLVAGRISPQTAINQLRGLARTSEPFLPARLPVIEQTWR"
     gene            4347..5513
                     /gene="radC"
                     /locus_tag="VN363_09270"
     CDS             4347..5513
                     /gene="radC"
                     /locus_tag="VN363_09270"
                     /inference="COORDINATES: protein motif:HMM:TIGR00608.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006281 - DNA repair [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA repair protein RadC"
                     /protein_id="HWQ84745.1"
                     /translation="MCQLLLLPDKSGNTKYTPKHKLLPLRERPVERVSARADACTIIE
                     LLAALLGGPQPIETADALLAHFNGRLSDLRRAPAHEIADIPGIGQQNAARIKAAVELG
                     LRLTLNTEEDRPTIHSPADAAAMLQHEMSALEQEHLRVILLDTRNRVIDIVEIYHGSL
                     NSAQVRVAELFKPAIQRMAAAIILIHNHPSGDPSPSPDDVAVTRAVVQAGKLLDIDTL
                     DHLVIGRGRFVSLKERGLGFDGRVSETKPGYRLRSARYSIHDDDDLIFAYTRSQALAD
                     GVLVDVSQMASEAGFRYPTAITADLHARITPNEREKALGQSYEGRLWDVVFLASFVAR
                     RSGTQDRCIIGVSLFEADEAPPHRTHRHTLQLWMVVGPGDQGEPVITIGFPEDF"
     gene            5524..6630
                     /locus_tag="VN363_09275"
     CDS             5524..6630
                     /locus_tag="VN363_09275"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84746.1"
                     /translation="MTGKSLPGLPAPFENTYFRERALKAANRQKQGHILVSGSKSDTG
                     QGLPLPYIHDVPGLRRGNYPYDYECEWGRFKYEYELGSYLFTPHNGQVPPGWEQYDLQ
                     TPIQPVSAVIDRARRLATVKTKDREIVLRDVPVGESPYNMLQQVNATLAQSCQPFVAW
                     RLEWITSVIDRLWPDGVPQIRNEHGSAYVTGYAYDDAGNLVYLGMVGHKTVLESIRAT
                     VQARQRKKLFLQGRPVCPLTSHYCQTWQHLPDYGAYHATLIADPALPGKWQPGEAVVY
                     LLVCEGELPDGSTPETWTRHLLTSRLSETLSIPILNEWEEHLWEVGQIENLILGLVTG
                     GDCQVGYIVQLDETGWKEVVVRLLKARKIRLSGD"
     gene            complement(6705..6986)
                     /locus_tag="VN363_09280"
     CDS             complement(6705..6986)
                     /locus_tag="VN363_09280"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84747.1"
                     /translation="MQKGKMPMKIGFVPISIEGYIELHLKDNPDAKKEEIIIALNEAL
                     EAYKRGAKCTNCGNPIWVIGSALAGNMCFTCITGEAIPEDDYEIDEACY"
BASE COUNT         1565 a         2067 c         1957 g         1468 t
ORIGIN      
        1 acagctcctg ctggagatgc gttccggcct ttttatttaa ccactttcta ggaggttacc
       61 atgatcaacg aagtcgttct cgaaggcatt gtcgtcaaga cctggaagta cgctgaagac
      121 ctgctcttcc gcattgcctg ctaccgcgat ccggatctgc cttccaagtc gctcaacgat
      181 ctccaggacg ccgctgattt cgtcaccatc cgcgtgctca agggcaacct gggcgcgccg
      241 gtggtggtcg agaagggagt tcacgtgcgg gtacacgggt tcttgcagag ccgggattac
      301 gaagagagtc tggcagactt cctgaaggat gcccgtggtg cggaactgcc ggttccagaa
      361 ggcctggatc cgaacgaact gcgcgccccg cgcggcacca cggaggtcat tgcccggcgc
      421 atcatgagcc agcgcaatgg atctaaaagg tgaccaattt gactgaatag cctttctcta
      481 ctagcggcga ttcgcaatca tccgatttat ctatcagggg gatatctcag aacaaccgaa
      541 taattctgtg acttatacgt aggccggtgt gcttccccca ggagggttgt gcaccggcct
      601 tgttgcttta acgagccttg cagctcgaag agcagcccgc accttgacaa acagcctctt
      661 tgcgaaaacg ttctgccctg gcaggcggtg gccgctgccc cttactccgg gggcgggtcg
      721 tgagaagcgc tggggaaact acgtttcctt aaagtcgtcc gcaaaggcca ggctcataag
      781 atgccaaata ccgatttcat cggctgcccg gttggcgtaa aaccaatcgg gcagtcaatc
      841 acagtttcag agcagcaaac tttgtcggct gagctatcgc ttggcggtca aggtttgctg
      901 ccctgacgtg ccctgaagtg aggaactcag ggaagcaatc gtcggtttgg aagctttata
      961 cagcttaccc aatcaacgtc ggaaaccaat ccccatacgt ttgttttgac aagaaaggag
     1021 cgtaatgcac acacgatgac tatcgcaccc cccatcctgg agaaccaact ccaacacgat
     1081 cgcatggtcc gggcgcaggc tgccctgatg aatggcgata tgcaggtgat ggtcttgact
     1141 gactgtacct ggatggttgc cagcaagagc agccgctaca ccgtttcgct ggatggcgat
     1201 gcctggacct gcacctgtct ggatttcacc ggacgctgcc agcgttttgg cttgcgctgc
     1261 aagcacatcg aggcggttcg tctcaccgag acagagaaga tcgccgagcc caggctggtg
     1321 aaccagtaca cgtatccaca gttttcaacc acttacacgg aggaacaaat gacccaatct
     1381 atgccaacac acgatccccc cacgctcgac ccgtccagtg agcagatcct gtggcgactt
     1441 aagcagccgc tggatatgaa ccgcgtcaag cgtcgccagg cacccgggca agggactgtc
     1501 ccttacctgg aaggctacga tgtgatcgaa gctgccaacg acctgttctt gttccgctgg
     1561 tcgttcgacc tgctcagtga gccgcatatt atgcgctggg ataaggtgat caccttttac
     1621 gatcagcgtg ccaggaagaa ggtgccggtt ctgggcgaag atggcaaacc aaccacggag
     1681 atcgccggca tcgcttacat caccggccgg atcacggtcg agctggatgg taaagccttc
     1741 agccatgccg acgcggggcg ctgcatcttc actggcgata cgcccgaagc cctggatatg
     1801 gccatcgccg gcgcggtcac cgactgtctg aagcgctgct tccgccagct cggagaacaa
     1861 ttcggaaatt ccttgtacga caaggaagtc gctaagactg ctggcctgga gaacggcgct
     1921 tcgaacggtc acggatcgaa cggcaacgga caatccgaaa aatcgaccgg gcaaacgaca
     1981 ccgcctaatc cgactgtgcc acccaatcca cccgcatccc aggcggaggc gctgcaatac
     2041 cgggatggcg tagcggtgga cacaagcaac gctgcggaat tggaggcttt caatgccttc
     2101 aagtccgccc atcaggagct ggctccagcc tcgcgcgaag tgctgcgcgc ctgggtcgcc
     2161 agccgcaacg gcaagaagta atcccatatt tgagaaacgg aggataaccc catgtcattc
     2221 caaacgatca tcatcgccgg tcatctcggc cgcgacccgg agatgcgcta tacccctgcc
     2281 gggcaggccg tgaccaactt caacctggct gctaaccgcc agtacaccga ctcgagcggt
     2341 caggtggtga aggaaaccac ttggttccgt gtttcggcct ggggcaagac tgccgaaaac
     2401 tgcaatcagt atctgcacaa gggcagcctg gtgctggtgg aaggccggct ggtgtgcgac
     2461 ccggaaacgg gcggcccacg gctctacact cgccaggatg gctcgcccgc ctcatccttc
     2521 gaagtgagcg ctagcacggt gcgcttcctg tcggggcgtg aagaagaagg cgaacaggaa
     2581 cctgcaggga atggctcggc tgcatctcag cacgatgaca ttccgttctg atccacacga
     2641 gattgccctg ggctcgcctg gggcaatctc aagcaattcc tcacacggag gtaaacatga
     2701 tcaagaagat ttaccgcggc gagccgggcg gtgcagtcaa agtcaccctg ccctccggca
     2761 aggcgcggcc cttgcgacac gtggtgctgc acagcccaac cgggttcggt tggggatacg
     2821 gcggctccgg accggcagac ctggcgctaa gcatcctatg cgatgtgctg agcgagcgac
     2881 caactgagaa gcagatctac cacgggcggt tcatagccta ccctcaccac caggatttca
     2941 agcgcgagtt cgtcgccagg tgggagtatg gcagcgggtt cgagattgac tcggaaacca
     3001 tctctgactg gctgcgcaag cggggcgtgg aggtgcagcc atgaccgatg agtacatctc
     3061 tgatgacggt ccaggctccg acttgtttga tccctatgag accggctggt ggaaatctat
     3121 ctccagcgat gactcagcgc ctgcagatca gccttgcccg aattgcaacg gcgaaggtta
     3181 tgttgtcgtc tgggtctatc cctgggacgg ctgttcgtac gatgaagagc cgtgtaagtt
     3241 ctgtaatccc aggaaagatc gctcagaact ggatgacgaa ccaataaaag agatggatat
     3301 ccccttctag atggttctca ccgttggcat tcaaattccc acacgttctg gaggtaagta
     3361 gtatgcaaga taccctgacc caaacaccca aggtcgatct gacaggcaga cccactcgca
     3421 tccgtgtgat cgggtacacc tacctggtcg atttcggccc ttcgactcaa ccccgttttc
     3481 acaccgtcaa caagcagcgc agctgctctt gtccgttgaa ggaaacctgc ccggccatcg
     3541 atgccgtggc ggagtacctg cgcaacggcg ggcaacgcgc ccccgatccc atgccgcctt
     3601 gcccgatctg cggtgcagag actgtccgcg atcgcaagtg ggacggcaaa tacaccaaag
     3661 aactcggctg gcgctgtacg gcaggaggtc ttcgacactt cctggaagcc aaagccgaac
     3721 gaataaaaga ggcgctacgc cgcaatcaag cagcggtcag cgaacacgaa agtgcagctg
     3781 gccgttgatt tatttaacgg aggaaatgat gcaagtacac acgttgaccc acatggaatt
     3841 cgagcctgca ccggtgcaag tcggtgagcc attccgctgt tgggagtgcg ggtcgccaga
     3901 tcagggcggt atgttcctga tcatcgacca ctccccgctg gaagcctaca ccgatcacgt
     3961 ctactacatg cgcctgtcct gcgggcacaa taaccgctgc gtgcgcctgg gcgacgccgt
     4021 tgccccgctg cgcccgctgg atcgctcgat tgacgagaac ctggcgagtg tgcgtgaagg
     4081 attggcgcac ctggcaaagg cggtcccacc cgatcagcag aaccaggagc ctgtctccaa
     4141 gatcgtctac ttgctggtcg ccggtcgcat ctcgccgcag acagccatca accagttgcg
     4201 cggcctggcg cgaaccagcg aaccgttctt acccgctcgg ctgcctgtta tcgaacaaac
     4261 ctggcggtaa ttccgcctca accaattcac acgccgcccg ctctggaacc ccccagggcg
     4321 ggcgatttca ttacacggag gaaaacatgt gccagttgct gttactgccg gataagtccg
     4381 gcaacacgaa atataccccc aagcacaagc tgctcccctt gcgggagcgc ccagtggagc
     4441 gggtcagcgc ccgagccgat gcttgcacaa ttatcgaatt gctggctgcc cttctcggcg
     4501 gtccgcagcc catcgaaact gccgatgcct tgctggcgca cttcaacggc cggctatccg
     4561 atctgcgccg cgctccggcg cacgagatcg ccgatatccc cggcataggg caacagaacg
     4621 ccgcccgtat caaagcagcc gtagagctgg ggctgcgcct gacgctcaat accgaggaag
     4681 atcgaccgac gatccacagc ccggcggacg cggctgcgat gttgcaacac gagatgagtg
     4741 ccctggaaca ggagcacctg cgggtgatcc tgctcgacac acgcaaccgg gtgatcgaca
     4801 ttgtggaaat ctaccacggc agcctgaatt ccgctcaggt gcgggtggcg gagctgttca
     4861 agcctgccat tcaacgcatg gctgcggcga tcattctgat ccataatcac ccctccggtg
     4921 atccgtcacc aagtcctgac gatgtggccg ttacccgcgc tgttgttcag gccggcaagc
     4981 tcctggatat cgacacgctg gatcacctgg tcatcggtcg cggtcgtttt gtctcgctca
     5041 aggagcgcgg tcttggtttt gacggtcgcg tttctgagac gaaacctggt tatcgcctgc
     5101 gatcagccag atattccatc catgacgatg acgacctgat ctttgcttat acgcgctccc
     5161 aagctctggc ggatggcgtg ctggtggatg tctctcagat ggcttccgaa gccggctttc
     5221 gctatccaac cgccatcacc gccgacttgc atgcccgcat cacccctaat gagcgggaga
     5281 aagcgctagg tcagagctac gaaggtcggc tatgggatgt ggttttcctg gcatccttcg
     5341 tcgctcgccg atcaggtaca caagatcgat gcatcatcgg tgtgagtctt ttcgaagctg
     5401 acgaggctcc tccgcaccgt acccaccgcc acacgctcca gttgtggatg gtggtaggtc
     5461 ccggcgatca aggtgagccg gtcatcacga tcggtttccc cgaggatttc tagggaggca
     5521 gccatgactg gaaaatcact accaggcttg cctgcccctt tcgagaacac ttacttccgg
     5581 gaacgcgccc tcaaagcagc caaccggcaa aagcaaggtc acatcctggt ctccggctca
     5641 aagtcagata caggtcaagg tttacccctg ccgtacatcc acgatgtccc cggcctgagg
     5701 cgtggcaatt acccctacga ttacgagtgt gaatggggcc gtttcaagta tgagtacgag
     5761 ttgggctcct atctcttcac accacacaat ggtcaggttc cacccggctg ggagcaatac
     5821 gatctgcaaa cacccatcca accggtttcc gctgtgatcg accgagcgcg ccgcctggcg
     5881 accgtcaaaa caaaggatcg agaaatcgtt ctgcgcgacg tgcccgtggg tgaaagcccc
     5941 tataacatgc tccagcaggt caacgctacc ctggcgcaaa gctgccagcc cttcgtggcg
     6001 tggcgtctgg agtggatcac cagcgtaatt gaccggttgt ggccggatgg cgtcccgcag
     6061 atccgcaatg aacatggctc ggcttatgtc acaggttacg cctacgacga tgccggcaac
     6121 ctggtctacc tgggcatggt cgggcataag acggtgctgg agtctatccg ggcgaccgta
     6181 caggccaggc agcgtaagaa gctcttcctg caaggccggc cagtgtgccc gctgaccagt
     6241 cactactgcc aaacctggca gcatctgccg gactatggag cataccatgc tacgctaatc
     6301 gctgacccag ccctgccggg caagtggcag ccgggggagg cggtggttta ccttcttgtg
     6361 tgcgaaggtg agctgccaga tggatcaaca ccagaaacct ggactcggca tttgctcact
     6421 tctcgtctat cggaaacatt atcgatcccc atcttgaacg aatgggaaga acatctatgg
     6481 gaagtcgggc agattgaaaa cttaattttg gggctggtaa ctggtggcga ttgtcaggtt
     6541 ggttacatcg ttcagttgga tgaaactggt tggaaggagg ttgtagtcag gttattgaaa
     6601 gcacgtaaga ttcgtctctc tggtgattaa tgagaaaaac cataacagga atgggtgcta
     6661 atccattcct gttatggttc actaattcgc tcagaacgag gtaatcagta gcaagcctca
     6721 tcgatctcat aatcatcttc tggaatcgct tcaccagtta tgcaggtgaa gcacatatta
     6781 ccggcaaggg ctgatccgat tacccagata ggatttccac aattagtgca ttttgcgcct
     6841 ctcttgtacg cttccaatgc ttcgttaagt gcgatgataa tttcctcctt cttcgcatct
     6901 ggattgtctt taagatgaag ttctatgtac ccttctatgc taatcggtac aaaaccgatt
     6961 ttcatgggca ttttaccttt ctgcatgatt tatttttctt acatctgcaa aaacttttca
     7021 tctccacacg cccagccaga atataccttg ataccaa
//
LOCUS       DATMXK010000507         3878 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_53580, whole genome shotgun sequence.
ACCESSION   DATMXK010000507 DATMXK010000000
VERSION     DATMXK010000507.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3878)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3878)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3878
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53580"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1406
                     /locus_tag="VN363_09285"
     CDS             <1..1406
                     /locus_tag="VN363_09285"
                     /inference="COORDINATES: protein motif:HMM:NF013871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84748.1"
                     /translation="SRPPTRQNLGARLEAALPPMRLQLLKAVVTTASELGVALYVVGG
                     FVRDLLLDRPSLDFDLVVEGDAIQLAHALGRRYGGRVTSHARFGTAKWHTGELREVLA
                     ARLEQVNTAPNQLEDNFSDLPDSLDLVTARTEFYTYPTALPTVERGSIKLDLHRRDFT
                     INTLAVRLDGEHYGDLHDYWGGLNDLRQKKIRVLHSLSFIDDPTRLLRAVRFEQRFGF
                     QIEPRTLELMKQARPLLERISGDRIRHELDHTLVDPKVTIILARLNELNLLQTIHPKL
                     VWDAWLETCLGKLQNYALTDDWEPGGNPNSVQIRQDIPYIFWTVRLANSEINNIAERL
                     RLSAGLRRVLFAARALWEHLPELVSQPVSEKTIYLDEIPGLARLGVYLASEDGQSSAM
                     LEQHAQKWSHIKPIADGRVLHQRGLLPGPIYKEILSQLRAGWLDGKIHTQSEEEALLN
                     SLLAGLPQTTGKSPDLL"
     gene            1403..1948
                     /locus_tag="VN363_09290"
     CDS             1403..1948
                     /locus_tag="VN363_09290"
                     /inference="COORDINATES: protein motif:HMM:NF012792.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84749.1"
                     /translation="MTYLRPVRQDWLSQVTIRHLEAGDLPALEWDGEYTHFRRLFQDA
                     YEYALQGRSILWVADLQGRGVIGQLFIQLFSGRRELADGNNRAYIYGFRIQPPYRRAG
                     LGTRMMNVAEQELIARDFQYVTLNVGHNNPDAKRLYETHGYQVVASEPGRWSYLDHEG
                     IRRDVDEPAWRMEKKLSPRVS"
     gene            2106..>3878
                     /gene="nusA"
                     /locus_tag="VN363_09295"
     CDS             2106..>3878
                     /gene="nusA"
                     /locus_tag="VN363_09295"
                     /inference="COORDINATES: protein motif:HMM:TIGR01953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006353 - DNA-templated transcription
                     termination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription termination factor NusA"
                     /protein_id="HWQ84750.1"
                     /translation="MKNEFTLAFNEVLEEKQLPQNIVLEALEAAMVSAYRRAVNASNA
                     QQVEASIDPETGKVIIYAEKEVVESVEDARTEVSIRDAQKVDPNAQIGGMVLVETTPR
                     NFGRVAAQTARQVIQQRIREAERDAQQHHFAKHQGEIVSGVVQAVNPSQITIGLDMKA
                     EGVMARQHQIPGERFRVHDRVRALLLEVKMTTRGPQINLSRAHRNFLRRLLENEVPEI
                     YQGMVEIRSIAREPGQRSKVAVAALQAGVDPVGACVGIKGVRIQAIVRELNDEKIDVI
                     EWNSDPAAYIAKALSPARVSGVFLDEHTKGAKTATVVVPEDQLSLAIGRDGQNARLAA
                     KLTSWRIDIKSLPEAASDALNKLNTDPQYAAIAETEKDILPQVEVILGKKAEGRPVTP
                     EEYMVLNQLVDRVERGILQKFQAEKQAEDDREQQARALIPAGAFEIPLEKFEVSDRVY
                     TVISEAGFPTVGELMYQMEIDPDSILKLSGMGPKGMKELEESLLSVKTELEAKAAEQA
                     AAELAAVAAEAEAVSELEPVVEPVSPPESVEAEVAVTELAETLQPEAGLVETELVEEG
                     AVETASAELAAEEPVTETVPEPAEA"
BASE COUNT          937 a         1030 c         1096 g          815 t
ORIGIN      
        1 attccagacc ccccacacgc cagaatctgg gcgcccgcct ggaggctgct ctccccccta
       61 tgcggctgca gctgctcaag gcggtcgtta caactgcctc tgagctaggc gtggcgcttt
      121 atgtggtggg tggatttgtg cgcgatttgc tactggatcg ccccagcctg gatttcgatc
      181 tggttgtcga aggagatgcc atccagctgg cacatgcact cgggcgccgt tacggaggca
      241 gggtcaccag ccatgcccgc tttgggacag cgaaatggca tacgggcgag ttacgggaag
      301 tgctggctgc gcgactggaa caagtaaaca ctgctccaaa ccagcttgag gataatttca
      361 gcgacctgcc ggacagcctg gatctggtga ccgcccggac tgagttttat acctacccaa
      421 cagccttacc aaccgtggaa cgcggcagca tcaaactgga cttacaccgg cgcgatttta
      481 cgatcaatac cctggcagta cgcctggatg gggaacatta tggcgatctg catgattatt
      541 ggggcgggtt gaatgatctg cgccagaaga aaattcgcgt gctccattca ctttctttta
      601 tcgatgatcc aacccgcctg ctgcgtgccg ttcgcttcga gcagcgcttt ggattccaga
      661 ttgagccgcg cacactggag ctcatgaaac aagcacgacc actgttggaa cggatatcgg
      721 gtgatcggat ccggcatgag ctggatcaca cgctggtcga tcccaaagta actataatcc
      781 tggcgcgatt gaatgagctc aatttactgc aaacgattca ccccaaactg gtctgggacg
      841 cctggttgga aacctgcctg ggaaaattac aaaattacgc tctaaccgat gattgggaac
      901 ctggtggcaa tccgaattcc gtccagatcc gtcaggatat accttatatc ttctggactg
      961 taaggttggc aaactccgaa atcaataaca tagcggagcg actgaggctc tctgccggat
     1021 tacgccgagt attgtttgct gcgcgagcat tatgggaaca ccttccggag ctcgtcagcc
     1081 agccagtcag tgaaaagacg atctatctgg atgaaatccc cggtctcgct cgtcttggcg
     1141 tgtacctggc tagcgaggat gggcaatcct cagccatgtt ggagcagcac gcccagaaat
     1201 ggagccacat caaacccatt gcggacggac gtgtgttaca ccagcgggga cttctcccgg
     1261 ggccaatata caaggaaatc ctcagccagc tcagggcagg ctggctggac gggaaaatcc
     1321 acacccagtc cgaggaggaa gccctgctta actcacttct tgccgggctt cctcaaacaa
     1381 ctgggaaaag ccctgacctt ctatgacgta cctccgacca gtccgacaag attggctctc
     1441 gcaagtcacg atccggcatc tagaggccgg cgatttgccc gcgctggaat gggatggcga
     1501 gtatacccat ttccgacgat tgttccagga tgcttacgaa tacgccctgc aagggcgctc
     1561 gatcctgtgg gtcgccgacc tgcaaggtcg gggtgtgata gggcagttat tcatccagct
     1621 gttcagtggg cggcgagagc tggcggatgg caataatcgg gcctatatct acggcttccg
     1681 gattcaacca ccttaccggc gggcgggttt aggtacccga atgatgaacg ttgctgagca
     1741 ggagctcatc gcacgggact tccaatatgt taccttgaat gtaggccata ataacccgga
     1801 cgccaaacgc ttatacgaaa cgcatggcta tcaggtcgtc gccagcgagc ctggtcgctg
     1861 gtcgtatctg gaccacgaag gtatccgccg ggatgtggat gaaccagcct ggcgaatgga
     1921 gaaaaaacta tctccccgcg tttcataatg ttttttggcc agagacgccc ttgcattgaa
     1981 tacgcaaata tgttaaaatc actccggtta gttcagcgat attagatccg gatgcatcga
     2041 aaagtgggca acccccactt ttctgcttat atcagtcaac aactccaggt ggagaaagaa
     2101 agagcatgaa gaacgaattt acgttagcat tcaacgaggt attggaagaa aaacagctgc
     2161 cccagaacat cgttctggaa gcactggaag ccgccatggt atcagcttac cgtcgagcag
     2221 ttaatgcttc caatgcccag caggttgaag cttccatcga tccggaaacc ggtaaggtga
     2281 tcatttacgc cgaaaaagag gtggttgaat cggttgagga tgcccgaacg gaagtctcga
     2341 ttcgggatgc ccaaaaagtg gaccccaacg cccagattgg cggaatggtc cttgtcgaaa
     2401 cgaccccccg caattttggt cgcgtcgccg cccaaaccgc tcgccaggtt atccagcagc
     2461 gcattcgcga agctgagcga gatgcccaac aacaccattt cgccaagcac cagggcgaga
     2521 tcgtcagcgg tgtggtgcaa gcggtaaatc ccagccagat tacgattggc ctggacatga
     2581 aagccgaagg ggtgatggcc cgccaacacc agatacccgg tgagcgtttt cgcgttcacg
     2641 atcgagtgcg ggcattactg cttgaagtca aaatgaccac ccgtggtccg cagatcaatc
     2701 tgtccagggc gcatagaaat ttcctccgtc ggctgctcga gaatgaggtt ccggaaattt
     2761 accaggggat ggtcgaaatt cgttcgattg cccgcgagcc tggccagcgc tccaaagtcg
     2821 ccgtagctgc cctgcaggca ggtgtcgatc cggttggtgc gtgtgtgggt atcaaaggtg
     2881 tccggatcca ggctatcgtc cgtgaattga atgatgagaa gattgacgtc atcgaatgga
     2941 attccgatcc ggctgcctat attgccaaag cgctcagccc ggcacgcgtc tccggggttt
     3001 ttctggatga acataccaag ggagcaaaga cagccacagt tgtcgtccca gaagaccagc
     3061 tgagtctggc gattgggcgc gacggtcaga atgcccgcct ggctgccaaa ctgacctcct
     3121 ggcggattga tatcaagagc ctaccggagg ctgcttcgga tgcgttgaac aagcttaata
     3181 cggatccaca gtacgcagcc atcgccgaaa ccgaaaagga tatcctgcct caggtggaag
     3241 taattctggg caagaaggct gaaggtcgcc cggtgactcc tgaagagtat atggtgttga
     3301 accagcttgt tgatcgggtt gaacgtggaa tcctccaaaa attccaggca gagaaacagg
     3361 ccgaagacga ccgcgagcag caggcaagag ctcttatccc tgctggcgca tttgaaatac
     3421 ctctggagaa atttgaggtt tccgaccggg tctatacagt catcagtgaa gccgggttcc
     3481 ccacggttgg cgagctgatg taccagatgg aaatcgatcc cgactcgatc ttgaagttgt
     3541 cggggatggg tcccaaaggc atgaaggagc tggaggaatc cctgctgagc gttaaaaccg
     3601 agctggaagc caaagcagcc gagcaagcag ctgcagaact ggctgctgtt gctgctgaag
     3661 ctgaggcggt cagcgaactc gagccggttg tcgagcctgt cagtccaccc gaaagtgttg
     3721 aagcagaggt ggcggtcaca gagctcgctg aaacattaca accagaagcc ggcctggttg
     3781 aaaccgagct ggtggaagaa ggtgctgttg aaaccgcttc ggcggaactt gccgccgagg
     3841 aaccagtcac tgaaactgtt cctgaacctg ctgaagcc
//
LOCUS       DATMXK010000508         2672 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51714, whole genome shotgun sequence.
ACCESSION   DATMXK010000508 DATMXK010000000
VERSION     DATMXK010000508.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2672)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2672)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2672
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51714"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2672
                     /locus_tag="VN363_09300"
     CDS             <1..>2672
                     /locus_tag="VN363_09300"
                     /inference="COORDINATES: protein motif:HMM:NF024883.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ84751.1"
                     /translation="FAAALWADGAIHKPWNEPRPNGLTATVAHTGELILVPDMSNHPL
                     FQDTPRDWNGAIIGVPLKFGNCVVGVMNIAFIQPRQFKDREVRMIRLLGDQAAIAIEN
                     ARLYQQAATERRHLSLLYQISRDVISSLDPDEVLERAIALTCQVLGGIVGQAFVYLPE
                     EGRLSLRAIYGRPAMNPAYLNLTYPMKPGVGLTGWVAKLRQPIFLEEVRDDPRWIHIP
                     GVDDDVHSAITAPILAGDELLGIFSVSHHRPGAFSEDHLELMMAICQEVGLALSNARQ
                     YQEVQHRLAEMTLLQNLSQILNQRLDPHQMLGEIARQLAAQAGYELVWIYQVEKGQPT
                     LAAQSSQYASSTSLSTPDELLQKVIASGQAAIQVIPNTLDQQLLPTSWACVPVFRQKS
                     VTGAICVASQQTRQLTAHDIDFLEVLGGQMSVALENTRLYEQILSHADELEVTVLQRT
                     NELTELYELSQEIGFSLSYENLLQLLLRRLQNAFRNGVVAGYLSTSETVPVTITVTRP
                     ISPAAVIQVRKIVEEALAESEREYLATHEPGVQLLASAEFEDLESLETVQLTNFITAP
                     IFTGKRLMGMLILASDQAVPLDTEQYRLLNTFANQARTAVQRLEAILTAQQEHLERLV
                     KNIPIGTVLLDENMKILVTNPLGQEILAVLSDQPASPNLSRLGNLPASELTLRQDDPL
                     PVVIELDGVPPRVFEAQIRPVQAEIHQWVLTLREVTRERESQARIQSQERLATVGQLA
                     AGIAHDFNNIMAAILVYTNLLGTDPKIADGSREKLDIIERQIQRAASLIRQILDFSRR
                     SVMEQTDIDLLPFIKELDQILERVLPENIRLVLNYLPGSYLVNVDPTRMQQVFMNLAL
                     NARDAMPQGGILQFELGRVSFDKRNPPPIADMPD"
BASE COUNT          599 a          777 c          710 g          586 t
ORIGIN      
        1 cctttgcagc tgcactctgg gcagacggtg cgatccacaa accgtggaac gaacctcgcc
       61 cgaacggcct gactgcaaca gtcgctcata ccggtgagct tattctcgtc ccggatatgt
      121 ccaaccatcc gctattccag gatacccccc gggattggaa cggggcgatc atcggggtac
      181 ccctcaaatt cggcaactgc gtggtgggtg tgatgaatat cgccttcatt cagccgcgcc
      241 agttcaagga tcgagaagtg cgcatgatcc gtctgttggg tgatcaggct gccattgcca
      301 tcgaaaatgc ccgtctgtac caacaggccg ccaccgaacg ccggcatctc tccctgctct
      361 accagatcag ccgggatgtg atttccagcc tggacccgga tgaagtattg gagcgggcaa
      421 tcgccctgac gtgccaggtt ttgggtggca ttgttggaca ggcctttgtc tatcttccgg
      481 aggaaggccg tttgagtctg agggccatct acggacgccc tgccatgaat ccagcctatt
      541 taaacctgac ttacccgatg aaacccggag tgggtttgac aggctgggtt gccaagctgc
      601 gccaacccat ttttctggaa gaggtacggg atgacccacg ctggatccat attcccggcg
      661 tggatgacga cgttcattca gcaattaccg caccgattct ggcaggcgat gaattactgg
      721 gtattttttc agtatctcac caccggccag gcgcattctc tgaggatcat ctggagctca
      781 tgatggcaat ctgccaggaa gtgggcctcg ctttgagcaa cgctcgccaa taccaggagg
      841 ttcagcaccg cctggctgag atgactttgc tacagaattt atcccagatc ctcaaccagc
      901 gcctcgaccc ccaccagatg ctgggcgaga ttgccaggca gcttgccgcg caggctggtt
      961 atgagctggt ctggatctat caggtcgaaa aaggtcagcc caccctggca gcccaatcca
     1021 gccagtatgc gtcttccacc agcttatcta cgccagatga actcctgcaa aaagtcatcg
     1081 cctctgggca ggcagccatc caggtcatcc caaatactct ggaccagcag cttttaccaa
     1141 cttcctgggc gtgcgtacct gtttttcgcc agaaatcggt taccggtgcg atctgcgttg
     1201 ccagccagca aaccaggcag ctcaccgccc acgatatcga tttcctcgaa gttttggggg
     1261 gacagatgtc ggtcgccctg gaaaacacgc gtctctacga gcagattctc tcccatgccg
     1321 atgaattgga ggtgaccgtc ctccagcgca ccaatgagct gaccgagctg tatgagctca
     1381 gccaggagat cggcttcagc ctatcgtatg agaatctctt gcagttactg ctgagacgtt
     1441 tgcagaacgc ttttcgcaat ggcgttgtcg cgggttatct atctaccagt gagacggttc
     1501 cggtaactat cacggtcacc cgccctatct ctcctgctgc agtgatccag gtccgcaaga
     1561 ttgtggagga ggccttagca gaatctgagc gggaatacct tgcaacccac gaaccaggtg
     1621 tacagctttt agcttctgca gaatttgaag acctggaaag ccttgaaact gtgcagctga
     1681 ccaatttcat aactgcgcct atcttcaccg gtaagcggtt gatggggatg ttgatcctcg
     1741 ccagcgatca agctgtacct cttgacacag agcagtaccg cctgctgaat acctttgcga
     1801 accaggcccg aactgctgtc cagcgcctgg aggccatcct gaccgcccag caggaacacc
     1861 tggaacgcct ggtcaagaac atccccatcg gtacagtcct gctggatgaa aatatgaaaa
     1921 tcctggttac caaccccctg ggccaggaaa tccttgcagt gctgtcagac caaccagcca
     1981 gccctaatct gtcccggctg gggaatctcc cggcgagtga attgacgctg cggcaagatg
     2041 atccgctgcc cgtggtgatc gagctggatg gagtgccgcc cagagtattc gaggcccaga
     2101 tccgacccgt acaggccgag attcaccaat gggtgctgac cttgcgtgaa gttacccgcg
     2161 agcgtgaaag ccaggctcgg atccagagcc aggaacgtct ggcaacagta gggcagttag
     2221 ccgccggaat tgcacacgat ttcaacaata ttatggcagc tattctagtc tacactaatc
     2281 tgctgggcac ggatccaaag atagccgatg gcagtcgcga gaaactggat atcattgagc
     2341 gccagatcca gcgggctgcc agtctgatcc ggcagatcct cgatttcagc cgccgctccg
     2401 ttatggaaca gaccgatatc gacttgctgc ctttcatcaa agagctggac caaatattgg
     2461 agcgtgtgct gcctgaaaac atccgcctgg tgctcaacta cctgcctggc tcgtatctgg
     2521 tcaatgtcga tcccacccgc atgcagcaag tattcatgaa cctggctctg aacgccagag
     2581 atgccatgcc ccagggcgga atcctgcaat ttgagctggg gcgggtgagt ttcgataaac
     2641 gtaatccacc gccgattgca gacatgcccg at
//
LOCUS       DATMXK010000509         2319 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_40690, whole genome shotgun sequence.
ACCESSION   DATMXK010000509 DATMXK010000000
VERSION     DATMXK010000509.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2319)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2319)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2319
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40690"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1736
                     /gene="glyS"
                     /locus_tag="VN363_09305"
     CDS             <1..1736
                     /gene="glyS"
                     /locus_tag="VN363_09305"
                     /EC_number="6.1.1.14"
                     /inference="COORDINATES: protein motif:HMM:TIGR00211.2"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009345 - glycine-tRNA ligase complex
                     [Evidence IEA];
                     GO_function: GO:0004820 - glycine-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006426 - glycyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="glycine--tRNA ligase subunit beta"
                     /protein_id="HWQ84752.1"
                     /translation="VDGGRYVTALVRQPGQPAVTVLAGALPGLIAGLKFEKSMRWNSS
                     NATFSRPVRYLLALYGNQVVPFEFAGLQAGTITRGLRSVTPDEISVADRQAYFSTLAA
                     QDILVDPVERKERVRQQVARLAAEVNGSIPDDPGLLAEVTNLIEAPTALRGSFDPAYL
                     ELPREVLVSVMKKHQRYFPVEKDGQLLPYFIAVRNGDERWLDQVIAGNEHVIRARFAD
                     AAYFIREDVRKPLAAYLPALDKLTFQLKLGSMLDKTRRIQSLVQDLLAGFALDDAEVR
                     TTLRAAELCKADLATQMVVEMTSLQGVMGQYYALGSGESEDVAAAILEHYLPRFTGDR
                     LPQSKPGLIIGIADRVDTLVGLFAVGLAPTGNKDPFAQRRAALGLVQNLLAKEVDLDL
                     RPVLAAAAGHQPVVVTPASLSAVLEFIIERLRYALLEQGARYDRVDAVLAVQGHNPAR
                     AARAVRELGYWAGRPDWNAILPAYSRCVRITRELGERYPVDAGSFNMAAENELYKALQ
                     KAESCQRREGSVEDFLNAFLPLMPQINQFFDQVLVMDENQEIRKNRLGMLQRIACLAD
                     GVADLSQLEGF"
     gene            1739..>2319
                     /locus_tag="VN363_09310"
     CDS             1739..>2319
                     /locus_tag="VN363_09310"
                     /inference="COORDINATES: protein motif:HMM:NF024987.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4140 domain-containing protein"
                     /protein_id="HWQ84753.1"
                     /translation="MPALITRINDVVVYVDRARITRLGSIAVEPGSLILEVSDLPGRL
                     NPESLRATAHGTARARLGGVQAQRQHPLEAPQEAVRELERDLEEVQDQRRMLEGRIEL
                     GNQNRQALVELLGETATYATALAAGELSLEKGLETLEALRARAERLDIEVQSLKVEKR
                     GIERREQQLVLAVQGHNPARAALAVRELGYWAGR"
BASE COUNT          470 a          609 c          725 g          515 t
ORIGIN      
        1 aagtggacgg gggccggtat gtcactgcat tagttcgcca gcctggccaa ccggcagtaa
       61 ccgtcctcgc tggtgccttg cccggactga ttgccggcct taagtttgaa aaatccatgc
      121 gctggaatag ctccaacgca actttttccc gtccggttcg ttatctgttg gcgctgtacg
      181 gaaatcaggt tgtgccattc gagtttgctg gtctgcaggc tgggaccatc acccgcgggc
      241 tgcgttcggt aaccccggac gaaatcagtg tggcagacag gcaggcctat ttttccacgc
      301 ttgccgccca ggatattctg gtcgatcctg tcgagcggaa agaacgcgtg cgccagcagg
      361 tagcgcgttt ggctgcggaa gtgaatggat cgataccgga tgatccaggt ttgttggcgg
      421 aagttaccaa tctaattgaa gcaccgaccg ccttgcgagg cagctttgat cccgcatacc
      481 tggagctgcc ccgcgaagtg ctggtctcgg tgatgaaaaa gcaccagcgc tattttccgg
      541 tcgaaaagga tgggcagctg ctcccttact ttatcgctgt ccgcaacggg gatgagcgct
      601 ggttggatca agtcatcgcg ggcaacgagc atgttattcg ggctcgtttt gcagatgcgg
      661 cttatttcat ccgcgaggat gtgcgcaagc cactggcagc atatctcccg gcgctggata
      721 agctgacctt ccagctcaaa ttgggctcaa tgctggataa gacccgccgt atccagtccc
      781 tggtgcagga tttgctggca ggatttgctc ttgatgacgc cgaagtcaga accacgctcc
      841 gggcagctga gctgtgcaag gcagacctgg cgacccagat ggttgtggag atgacctccc
      901 ttcagggagt catggggcag tattacgctt tggggtcggg cgaatcggaa gacgttgcag
      961 ctgcaattct ggaacattat ttgcctcgtt ttactggcga tcgtctgccg cagagtaaac
     1021 caggcctgat catcgggatc gccgaccggg tggacaccct ggtgggatta ttcgcggttg
     1081 gcctggcgcc gaccggtaat aaagatccct ttgcccagcg ccgggctgcg ctgggactgg
     1141 tccagaatct cctggcaaag gaggtggatc tggacctgcg gcctgttttg gctgctgctg
     1201 ccggtcacca gccagttgta gtcacccctg ccagcctgtc ggcagtattg gagttcatca
     1261 tcgagcgttt gcggtatgcc ctcctggagc agggcgctcg ttatgacagg gttgatgcgg
     1321 ttctggcagt ccaagggcac aatccagccc gggcagcgcg cgctgttcga gagttggggt
     1381 attgggctgg tcgtccggat tggaatgcca ttttgccggc ctactcccgc tgcgtgcgga
     1441 ttacgcgcga gctgggtgag cgttatccgg tggatgcagg aagcttcaat atggcggcag
     1501 aaaatgagtt atataaagct ttacaaaagg ctgaatcctg ccagcgtaga gaaggctctg
     1561 tagaagactt tctcaatgca tttcttccgc ttatgccgca gattaaccag ttcttcgatc
     1621 aagtcctggt catggatgaa aatcaggaga tccgcaaaaa ccgcctgggt atgctgcagc
     1681 ggatagcctg cctggcggat ggcgtcgcag acttatccca gctggaagga ttttagaggt
     1741 gccagctctt atcacgcgga tcaatgatgt ggtggtttat gtcgatcgag ccaggatcac
     1801 ccggctcggc agcattgctg tcgaacccgg atccctaatc ctggaagtct cggatttacc
     1861 tggcaggctg aatcctgaat cactgcgcgc caccgcccac ggcacagccc gggcgcgcct
     1921 gggtggcgtt caggctcaac gccaacaccc gctagaagct ccccaggaag ctgtgcggga
     1981 gctggaaagg gacctggaag aggtccagga tcagcgccgg atgcttgaag gccggattga
     2041 attagggaat cagaaccggc aggcattggt agagctgctg ggtgagactg caacttatgc
     2101 caccgccctg gctgcgggtg agttgtccct tgaaaaaggc ctggaaacac tggaagccct
     2161 gcgagcccgg gcagaacgcc tggatattga ggttcagtct ctaaaggtgg agaagcgcgg
     2221 gatagaacgg cgggagcaac aactggttct ggcagtccaa gggcacaatc cagcccgggc
     2281 agcgctcgct gttcgagagt tggggtattg ggctggtcg
//
LOCUS       DATMXK010000510         3360 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_146300, whole genome shotgun sequence.
ACCESSION   DATMXK010000510 DATMXK010000000
VERSION     DATMXK010000510.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3360)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3360)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3360
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_146300"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1242)
                     /locus_tag="VN363_09315"
     CDS             complement(<1..1242)
                     /locus_tag="VN363_09315"
                     /inference="COORDINATES: protein motif:HMM:NF015859.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PAS domain S-box protein"
                     /protein_id="HWQ84754.1"
                     /translation="MSGVTHPKPALAFLGKIRIPWWIAIVTGILLFIIWLQAISLYKT
                     SLIAEYRSQTSNEVGLLGNTLSLEVNRRVSLLTGLSAFAQTDSGNPGFEGRFTRFAQK
                     IYLSTPGIRMVALAPANKLEYVFPLTGNSAAVGYEPLKDQRPIVREDMERMAVLKQLV
                     ISGPVDLIQGGKGLIARQPIYEGDKYWGFAEISINLDTSFDWSVFDVENHTLAFVLLD
                     TRGKLISGDLEILNQQPVIQAVDLLDGTWKLAGIPKGGWQAVIAQPLDIFQAIGLLLV
                     GLVTLSVYLISDQQWRMARAVKERTRELTAVNSRLRDGIRERLLINEALRSSEEQLSN
                     ILRVAPDAIVTCSKSGSVLLFNHAAEDILGYPADEVLGKSITEIFPDKVTQWIGDRDQ
                     SEIHKDHLVSINNVPFIGQHRD"
     gene            1422..2870
                     /locus_tag="VN363_09320"
     CDS             1422..2870
                     /locus_tag="VN363_09320"
                     /EC_number="1.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF019604.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)/FAD-dependent oxidoreductase"
                     /protein_id="HWQ84755.1"
                     /translation="MLDMIVIGGGPAGVTAALRARELGAEVALIERDRLGGTCTNDGC
                     VPTRVLAKAARLARDAQQFPDYGLGTYFPQVDFERLMSRTQQRVYRIHEKKQIINHLQ
                     DNGIQVYSDAGPARFIDSHTVELAGNRTLQASQFIISAGGHARRINFPGSELALTHSD
                     IWGLKKLPRSLAIVGGAATGCQLASIFSAFGTEVTILEMHPYIIGLEDDLVSATITEA
                     FLRRGIQVITQIGGVDRIERVGDELQLWYQLNGQAQSLQAEAIVLAVGWVGNIESMNL
                     EAAGVRTERGYIVVNDNLQTSASHIFAAGDITGRMMLVQSGSYDGRIASENAILGVQQ
                     PYRHQIVPHGGFTDPEYGSVGLTEKQARESGMDITIAIVPYQDLDRAVIDGRTEGMCK
                     LIINTDTHRIVGAHIVGEQALETIHLVAAGMAADMWVEQLAELEIAYPTFTASIGLAA
                     RQIVRALGVMPLAAQWRSLGKPHAEWEHSSPA"
     gene            2901..>3360
                     /locus_tag="VN363_09325"
     CDS             2901..>3360
                     /locus_tag="VN363_09325"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84756.1"
                     /translation="MSTFDARHEQRQTLSPRYAFEQDRSITRWYVLVVFIIAGLTLIM
                     APILALSWKQIPFPGFVTEQTLVVANINGIGWTGRQAGINYPERIVNFAEFTIRSRSD
                     FDRAKWEYPPGHHVRIRTVAPDGTFSDYRAIQMMEFPDPDFVRFFWLPYII"
BASE COUNT          830 a          864 c          846 g          820 t
ORIGIN      
        1 gtctcgatgc tgcccaatga agggaacgtt atttatggaa accagatgat ctttatggat
       61 ctcagactgg tcccgatctc cgatccactg ggtgacttta tcaggaaata tctcggttat
      121 gcttttgccc agaacttcat cagctggata tcccaggatg tcttccgcag catgattaaa
      181 cagcaaaacg cttccagatt tcgagcaggt gacaattgca tcgggcgcaa ctcgaagaat
      241 attggataat tgttcttcac tactccgtaa agcctcattg atgagcaaac gctcccggat
      301 cccatctcgg aggcggctgt ttacagcagt taattccctt gtccgctcct taacagcccg
      361 agccattctc cactgctggt ctgatatcag ataaacagac agggtcacca acccgaccag
      421 gagaagcccg atcgcctgga aaatatccag tggttgcgca atcacagcct gccagccccc
      481 ttttggaatc cctgccagct tccaggttcc atccagtaaa tcgacagcct gaattacggg
      541 ctgttggtta aggatctcta aatccccgga gatcagcttg cctcgggtat ccaacaaaac
      601 aaaagccagg gtgtggttct cgacatcaaa caccgaccaa tcaaaagagg tgtccaggtt
      661 gattgaaatt tcagcaaatc cccaatattt atccccctca taaattggct ggcgggcgat
      721 taaacctttg ccgccctgga tcaaatcgac cggaccactg atgaccagct gcttcaaaac
      781 tgccattcgc tccatatctt cacgcacaat cggacgttgg tctttaaggg gctcgtaacc
      841 aacggctgcg ctattcccgg tcaacggaaa aacatattcc agcttatttg ctggcgccag
      901 cgcaaccatg cggatacccg gtgtgctgag ataaattttt tgagcaaatc gcgtaaaccg
      961 gccttcgaag ccggggttgc cagaatctgt ttgcgcaaag gcagatagac ctgtcagcaa
     1021 agatactcgc cggtttacct ccagcgagag cgtgttgccg agtaaaccaa cctcattgct
     1081 cgtttgagat ctatactcag cgatcaggct cgttttatac aggctgatcg cctggagcca
     1141 gatgataaac aacaggatgc ctgtaactat ggcaatccac cagggtatcc tgatcttgcc
     1201 taaaaacgct aaagcaggtt ttggatgtgt gacgccagac atatattcct atgatgaatc
     1261 actccccagc atctattctc aaattatccc tattttatcc taattagtcc aggtgtatct
     1321 atttttgtgt ccattcctgt accagttgct gatacccttg atagtaaatc gaatggttta
     1381 atccagggta gaaatcaagc gttcatagca ggagattaac gatgctggac atgattgtaa
     1441 ttggaggcgg acctgccggt gttactgcgg cattgcgagc ccgggagtta ggcgctgaag
     1501 ttgctttgat tgagcgagat cgtttgggtg gaacctgcac aaatgatggc tgtgtgccaa
     1561 cccgggtgct ggcaaaagcc gctcgtctcg cacgcgatgc tcaacaattt cccgattatg
     1621 gcctggggac gtattttcct caggtggact tcgaacgttt aatgtctcgc acccaacaga
     1681 gggtataccg catccacgag aaaaaacaaa tcatcaacca cctgcaagat aacggtattc
     1741 aagtctattc cgatgcggga ccagcccgat tcattgattc acatactgtc gagctggccg
     1801 gaaacaggac ccttcaagcc tcacaattta tcatcagtgc aggcggacat gcccgccgga
     1861 tcaacttccc cggcagtgag ctggcattga cccattctga tatatggggt ttgaagaaac
     1921 tcccccgctc cctggcaatc gtgggtggcg cggcaactgg ctgccagctg gcgtcgatat
     1981 tcagtgcctt tggaacggaa gtaacgattt tggaaatgca tccttacatt atcgggctgg
     2041 aagatgatct cgtttcagcg accataaccg aggcgtttct caggcgagga atccaggtca
     2101 tcactcaaat cgggggggta gatcggatcg aacgtgtggg tgacgagctt caactctggt
     2161 accaacttaa tggacaggcg caatctttgc aagcagaagc cattgttctt gcagtcggct
     2221 gggtagggaa tatcgaaagc atgaacctcg aagctgccgg cgtcagaacg gagcgaggct
     2281 atatagtcgt caatgataat ctacagacct ctgcgagcca tatttttgcc gccggagata
     2341 tcaccgggcg gatgatgctt gtccagagtg gcagttacga tgggcgcatc gcttccgaga
     2401 atgcaatcct tggagtccag cagccctacc gtcaccagat cgttccgcat ggcggcttta
     2461 ctgaccctga atatgggagt gtcggtttga cggaaaaaca ggcccgggaa agtggaatgg
     2521 atatcacaat cgcgatcgtc ccgtatcaag atctcgaccg ggcggtcatc gatgggcgaa
     2581 cggaagggat gtgtaaattg atcatcaaca cagataccca ccggattgtt ggcgcccata
     2641 tcgttggcga acaggctctc gaaacaatcc acctggtagc tgctggtatg gcagccgata
     2701 tgtgggtgga acaattagca gagctagaaa tcgcgtaccc taccttcaca gcctcgatag
     2761 ggttagcggc gcggcaaatc gtacgtgcgc tgggcgtgat gccgttagca gcccaatggc
     2821 gcagcctggg caaaccacac gctgagtggg agcattccag tccagcttga gttgtattcg
     2881 agatttccgg gaattaatag atgtcaactt ttgatgccag gcacgaacaa aggcaaacgc
     2941 tctccccgcg gtatgctttc gagcaagacc gctctatcac acgctggtat gtcctggttg
     3001 tgttcattat cgccggcctg accctgatca tggcgcctat tctggcgctt tcctggaagc
     3061 aaattccctt tcccggattc gtaaccgaac aaaccttggt tgtggcaaat atcaatggca
     3121 ttggatggac tggacgccag gctggcatca actatccgga gcggatcgtc aattttgcag
     3181 aatttacgat ccgctcgcgc agtgattttg atcgagccaa gtgggaatat ccacccggac
     3241 accatgttcg gatccggact gtcgcaccgg acggtacttt ttcggactac cgtgcgatcc
     3301 agatgatgga atttcctgat ccggattttg tgcgattctt ctggttgccg tatattatcg
//
LOCUS       DATMXK010000511         2030 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_54176, whole genome shotgun sequence.
ACCESSION   DATMXK010000511 DATMXK010000000
VERSION     DATMXK010000511.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2030)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2030)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2030
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_54176"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..821
                     /gene="ppk2"
                     /locus_tag="VN363_09330"
     CDS             <1..821
                     /gene="ppk2"
                     /locus_tag="VN363_09330"
                     /EC_number="2.7.4.1"
                     /inference="COORDINATES: protein motif:HMM:TIGR03707.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005575 - cellular_component [Evidence
                     IEA];
                     GO_function: GO:0008976 - polyphosphate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0006793 - phosphorus metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="polyphosphate kinase 2"
                     /protein_id="HWQ84757.1"
                     /translation="TERIHIHSEVKEKQPKMKRQEYEQEIARLHIELVKLQEWIKARG
                     LKVVVIFEGRDAAGKGGAIKAITESLNPRICRVVALGTPTDRDKTSWYFQRYVAQLPA
                     GGEMVLFDRSWYNRANVERVMGFCTEAEYQEFMRSCPEFERMLVRAGIILVKYWFSVS
                     DEEQEKRFQARLDNPTKRWKLSPMDLESRRRWVQYSAAKDEMFKYTDTKQCPWYVVNS
                     DDKRRARLNMIAHLLSLIPYEDLTPESIILPEREKDSGYVRPPITDQTFVPEKY"
     gene            1015..>2030
                     /locus_tag="VN363_09335"
     CDS             1015..>2030
                     /locus_tag="VN363_09335"
                     /inference="COORDINATES: protein motif:HMM:NF016763.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005576 - extracellular region [Evidence
                     IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA];
                     GO_process: GO:0071555 - cell wall organization [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="VanZ family protein"
                     /protein_id="HWQ84758.1"
                     /translation="MINELLVLAITVFAWIIYRGAIWRRAWRRKASLSIGRELVVNLL
                     FVYLLVVFHLTFFPMNIVLYSYGPYKANFIPLVKTIHMIRYINPLVVVNILGNLVLLA
                     PLGIFLPVLSPRFRSWGAILGAGFLVTLTIEVFQYFLAVRVFDIDDLIINTLGVALGY
                     ALFLLLKKVPFVARRLERSPGQSQTENITPLLVFCGFGLLSFLALFFSQIWSQTKTRS
                     MLLDDLRKEEQQILAQGASGRYFFTFSETLSGAKKVDYYRQVILDRYTPVEYIEGLRL
                     AEEEFRVSGTSYDGEVTDFMVIAHSDQDIAAMTSQAARFPVFASDEYYFSFASLPVDQ
                     PDAY"
BASE COUNT          439 a          564 c          566 g          461 t
ORIGIN      
        1 ccactgagcg catccacatc cacagcgagg tgaaagaaaa gcagccgaaa atgaagcgcc
       61 aggaatatga gcaggagatc gcccggctgc acatcgagct ggtcaagctc caggagtgga
      121 tcaaagcccg cggcttgaag gtcgtggtga tcttcgaggg gcgggacgca gccggcaagg
      181 gtggagcaat caaggcgatc accgaaagcc tgaacccacg catctgccgg gtggtagcac
      241 tgggcacacc gaccgaccgg gataaaacct cctggtattt ccagcgctat gtagcccagc
      301 tgcctgccgg gggtgagatg gtcctgtttg accgctcctg gtacaaccgg gccaatgtgg
      361 agcgagtgat gggcttctgc accgaagctg aataccagga atttatgcgt tcttgcccgg
      421 aatttgagcg catgctggtc cgcgccggga tcatcctggt caaatactgg ttttcggtca
      481 gcgatgaaga gcaggaaaag cgtttccagg ctcgcctgga taatcctacc aaacgctgga
      541 agctcagccc gatggacctc gagtcccgcc ggcgctgggt gcagtactcg gctgccaagg
      601 atgagatgtt taagtacacc gataccaagc agtgcccctg gtatgtggtc aattccgacg
      661 ataagcgccg cgcccgcctg aatatgatcg cgcacctgct cagcctgatc ccatatgaag
      721 atctgacccc cgagagcatt atcctgccgg agcgggaaaa ggactccggt tacgtgcgcc
      781 caccgatcac cgaccagacc tttgtccctg agaaatacta aaatcaacct gccgcccggg
      841 ggaagtgcca gaccagctcc cccgggtgtt tgttttaaac gacaagtatc cccttgaatc
      901 gatcaaatcc tggcatgaac gccctccccg ggcggagggt atacttacta tcagccggct
      961 tacctgcaaa ccgtaaaatc ggctaaccgg tggatcggag gaaatctttt ggtcgtgatc
     1021 aatgaactgc tggtcctggc gataaccgtc ttcgcctgga tcatttaccg gggagccata
     1081 tggcggcgcg cctggcgccg gaaagcaagt ttaagtattg gacgcgagct ggtggtcaat
     1141 ctgctctttg tctacttgct ggtcgttttt cacctgacct tcttccctat gaacatcgtg
     1201 ctctacagct acggcccgta caaggccaat tttatcccgc tggtcaaaac aatccacatg
     1261 atccgctata tcaacccgct ggtggtggtt aacatattgg ggaatctggt cctgctggcg
     1321 ccgctgggca tttttctgcc ggtgctctcg cccagattcc ggagctgggg cgccatcctg
     1381 ggggcgggct ttctggttac tttgaccatc gaggtttttc agtacttcct ggcggtgcgg
     1441 gtgttcgata tcgatgatct gatcatcaat acactgggag tggcgctcgg ctatgcgctc
     1501 ttcctgctcc tgaaaaaagt cccgtttgtg gcccgcaggc tggaaagatc acccgggcaa
     1561 tctcagacag aaaacataac cccgctgctg gttttctgcg gttttgggct gctttcattc
     1621 ctggcgctct tcttctcgca gatctggagc cagaccaaaa cccgatccat gctcctggat
     1681 gatctgcgca aggaagaaca gcaaatcctc gctcagggcg cttcgggcag gtatttcttc
     1741 acattttcag aaacgctctc gggcgcgaaa aaggtggact actatcgcca ggtgattctc
     1801 gatcgctata cccccgtcga gtatattgaa ggcttacggc tggcagagga ggaatttcgg
     1861 gtaagtggga cttcttacga tggcgaagta acggatttta tggtgatcgc ccacagcgat
     1921 caggatatcg ccgccatgac ctcccaggcg gcgcgtttcc cggtctttgc gagcgatgaa
     1981 tattatttta gctttgccag cctgccggtt gaccaaccgg acgcctattt
//
LOCUS       DATMXK010000512         2187 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_151842, whole genome shotgun sequence.
ACCESSION   DATMXK010000512 DATMXK010000000
VERSION     DATMXK010000512.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2187)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2187)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2187
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_151842"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1928)
                     /locus_tag="VN363_09340"
     CDS             complement(<1..1928)
                     /locus_tag="VN363_09340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84759.1"
                     /translation="MRPPFSHPRRVPWNGVLLLLAFLLPAASALSAPAAQSTGLTLDS
                     RAGFDGLCKDQHWIPLRITVENSGPPISGQLLVSAPSYTNISTEYTTALELASSARKE
                     LRLAIYPETYINELNVRVIAAGQLILEQDIPVRCLALDQPLVGVLAGNPSGLNVLSEY
                     PAGAANANLARLELGDLPDQAQYLEALDVLLFSDLDSGALRPPQVQAIEGWLASGGQL
                     LVMGGAGWQKSAAGLGALLPFQPDGLVEINDLTALAGLAGVDAAPPAPALAATGSLTP
                     DAAALLRGEGQVLAATRPVGYGRAIYLAFDLASEPLRSWSAAPDLLAALLEPAPAVPG
                     WASGFIDSYQAGSALTTLPSLRLVGAGWLLSFLIVYVLAIGPLNYLLLRLLKRRELAW
                     VTIPVLALVFAAGAFLIGGRSRGALPVLSRLGVVQVWPGQAEARLDGLLGVFSPARQS
                     YTLSLAPGLTLHPLRDSNLQPPGGGYHLAVGEDGSLAMPELRMDVAGVLGQVVGGMLP
                     APQFEQDLQLILTAASARLEGKVTNLSGLKLENVVLFGPGFTQALGDLAPGAELALDV
                     DLQSAAWSNAAASPIKGTPGPYYYSYPSSSSAIDVLVGGVDYYQNAALFRRFSLLNAI
                     AGYNLDFGQAGGYYLAGWS"
BASE COUNT          450 a          688 c          719 g          330 t
ORIGIN      
        1 gaccagcccg ccagataata gccgccggcc tgcccgaaat cgaggttata accggcgatg
       61 gcattcagca ggctgaagcg gcgaaagagg gctgcgtttt gataataatc caccccaccc
      121 accaggacgt caatcgccga cgagctggat gggtaggagt aataatacgg acccggcgtg
      181 cccttgatcg ggctggccgc cgcgttggac caggccgcac tctgcagatc cacatccagg
      241 gccagctccg ccccgggagc caggtcgccc agggcctggg taaatcccgg tccaaacagg
      301 accacattct ccaacttcag accggagagg ttggtaacct ttccttccag ccgggcagac
      361 gccgcggtga ggatgagctg cagatcctgc tcaaactgcg gagcggggag catcccgccg
      421 accacctggc caagcacacc ggctacatcc atgcgcagct cgggcatggc caggctgcca
      481 tcttcgccaa ccgccaggtg atatccaccg ccggggggct gcagattgga atcgcgcagg
      541 gggtggaggg ttaatccggg cgccaggctc agggtatagc tctggcgggc cggcgaaaag
      601 acccccagca ggccatccag gcgcgcctcg gcctgcccgg gccagacctg gacgacaccc
      661 aggcggctga gcaccggcag cgccccgcgt gaacgcccgc cgatcaggaa tgccccggcg
      721 gcgaagacca gggccaaaac gggtatggta acccaggcca gctcgcggcg cttgagcagc
      781 cgcagcagca ggtaattcaa cggcccgatg gcaagaacgt aaacgataag gaagctgagc
      841 agccagccgg cacccaccag gcgcagactg ggcagggtgg taagggcgct gccggcctgg
      901 tatgaatcga tgaaaccact cgcccaaccc ggtacggcag gtgcaggctc gagcagggca
      961 gccagcaggt cgggggcagc gctccaggag cgcagcggct cggaagccag atcgaatgcc
     1021 aggtaaatgg cgcggccgta acccaccggg cgggtggcgg ccagcacctg gccttccccg
     1081 cgcagcagcg cggctgcatc cggggtaagg ctgccggtcg ccgcaagggc aggagccgga
     1141 ggagctgcgt ccacacctgc cagccctgcc agtgcggtca gatcattgat ttccaccagc
     1201 ccatctggct ggaaggggag cagggcgccc aggccggcgg cggatttttg ccagccagcc
     1261 ccacccatca ccagcagctg cccgccggag gccagccagc cttcaatggc ttgcacctgg
     1321 ggaggacgga gcgccccgct gtccagatcg gagaacagca aaacatccag ggcttccaga
     1381 tactgggcct gatcgggcag gtcgcccagc tccaggcgcg ccagattggc attggcggct
     1441 ccagccgggt attccgacag tacattcagc ccggagggat tgcctgccag gacacctacc
     1501 aggggctggt ccagcgccag gcagcgcacg gggatatcct gctccaggat cagctgcccg
     1561 gcggcgatca cgcgcacatt cagctcgtta atataggtct cggggtaaat cgccaggcgc
     1621 agctctttgc gagccgagga tgcgagctcc agcgcagtgg tgtactcggt tgagatattg
     1681 gtgtagctgg gtgcagacac cagcagctgg ccggagatcg gcgggccgct gttttctacg
     1741 gttatgcgca ggggaatcca gtgctggtct ttacacaggc catcgaagcc agcccggctg
     1801 tccagcgtca gcccggtgga ctgcgcggct ggcgcagaaa gcgcgctggc ggccggcagc
     1861 aaaaaagcca ggagcagcag cacgccattc cagggcacgc ggcggggatg ggagaaggga
     1921 ggtctcatcg gcttatatgg acatttatac tacactttcc agtacagacc ggaccagtgc
     1981 aggctggagt atagacgcag gcagaagccg gagggttccc ctccgcttat tttcaggggg
     2041 cgcgaaactg ccggaccctt gagaaaggat ccggcaggct ttgattggat agcaggtttc
     2101 ctgccaggtt agcgtgcttt agcccacgct agccaggctg ctgcatggtc tccatctcga
     2161 tcaattgctt gcaatacaca ttacagc
//
LOCUS       DATMXK010000513         2419 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_161607, whole genome shotgun sequence.
ACCESSION   DATMXK010000513 DATMXK010000000
VERSION     DATMXK010000513.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2419)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2419)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2419
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_161607"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1917
                     /gene="uvrB"
                     /locus_tag="VN363_09345"
     CDS             <1..1917
                     /gene="uvrB"
                     /locus_tag="VN363_09345"
                     /EC_number="3.1.25.-"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560394.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009380 - excinuclease repair complex
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0005515 - protein binding [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006289 - nucleotide-excision repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="excinuclease ABC subunit UvrB"
                     /protein_id="HWQ84760.1"
                     /translation="TGTGKTFTMASLIQQMQMPALVMAHNKTLAAQLYAEFKEFFPEN
                     AVEYFVSYYDYYQPEAYVPRHDLYIEKESEINEEIERLRLAATTSLMSRKDVVIVASV
                     SCIYGIGNPESYGRVVINLQSGNIYRRNALLRQLVESQYQRNDLELRPGTFRVRGDTL
                     DVLPAYQDRNGYRITFFGDEVERMIEFDAVTGELQRELTSAAIYPAKHFITDEEKLKQ
                     AIGDIEAELAERLAQLKSEDRYLEAQRLEQRTMYDLEMLREVGYCAGIENYSRHLDQR
                     PPGSAPWTLMDYLPSEYLLVIDESHMSVPQIRGMFNGDRSRKTTLVEYGFRLPSALDN
                     RPLKFNEFEQRMGYTIYTSATPGPYELGRAEQVVQQIIRPTGLVDPQVEVRPTEGQID
                     NLVGEIRWRVEQGQRTLVTTLTKRMAEKLADYLLELGTKVHYLHSEIDTLERIGILRD
                     LRLGVFDVVVGINLLREGLDLPEVSLVAILDADKEGFLRSGTALIQTIGRAARHVNGR
                     VIMYADRMTDSMKFAIDETNRRRAIQEAYNEEHGIEPVSIIKAVHDLTEQMSVHSVAE
                     PQGGYRVKQDTPKAEITRVIAEMEKQMKDAARNLEFEKAAILRDQIYELRTILADDPK
                     VKPWEKFKLLSGEE"
     gene            complement(1942..>2419)
                     /locus_tag="VN363_09350"
     CDS             complement(1942..>2419)
                     /locus_tag="VN363_09350"
                     /inference="COORDINATES: protein motif:HMM:NF024273.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ClbS/DfsB family four-helix bundle protein"
                     /protein_id="HWQ84761.1"
                     /translation="YEQIILKLSEAQILDTAMDNGSSFKDLLAHLSCWERIELYWLES
                     TLEGKQPERYYPGFEVGEHDPEGVINLLNSRIYQKFKDAPLEKVLEDFRSTHLRLVAL
                     VESLSEDMLNNPLAFSWWRGEAVMTSIAHNSYQHFHEHLNAVRSWLKQALGTPSVP"
BASE COUNT          556 a          666 c          712 g          485 t
ORIGIN      
        1 accggcaccg gcaaaacctt taccatggca tcgctgatcc agcagatgca gatgccagcc
       61 ctggtgatgg cccataacaa gaccctggca gctcagcttt atgccgaatt taaagagttc
      121 ttccccgaga acgccgtaga atacttcgtc tcgtattacg attactacca gcctgaagcc
      181 tacgtgccgc gccacgacct gtacattgag aaagaatcgg agatcaacga agagatcgag
      241 cgcctgcggc tggccgcgac aacctccttg atgtcacgca aagatgtcgt gattgtggca
      301 tcggtttcgt gcatctacgg gatcggcaac cctgaatctt acggccgggt ggtgatcaac
      361 ctccaatcgg ggaacattta ccggcggaat gccctgctgc gccagctggt ggagagccag
      421 taccagcgca atgacctgga gctgcgcccc ggcactttcc gggtgcgcgg cgataccctg
      481 gatgtcctgc cggcttacca ggaccggaac ggctaccgga tcactttttt tggcgacgag
      541 gttgagcgga tgatcgaatt cgatgctgtg actggcgaac tgcagcgcga gctgaccagc
      601 gcggcgattt accccgccaa acacttcatc actgatgaag aaaagctgaa gcaagctatc
      661 ggggatatcg aagcggagct ggcggagcgc cttgcgcagc tcaaaagcga ggatcgttat
      721 ctggaagctc agcgtctgga gcagcgtacc atgtacgacc tggagatgct gcgcgaggta
      781 gggtactgtg ccgggatcga gaactattct cgccatctcg accagcgccc cccaggttcg
      841 gcgccctgga cattaatgga ttacctgccc agcgaatacc tgctggtgat cgatgaatcg
      901 catatgtcgg tgccccagat ccggggcatg ttcaatggtg accgctcgcg caagacgacc
      961 ctggtggaat acggtttccg cctgccttcg gcgctggata atcgcccgct gaagttcaac
     1021 gagttcgagc agcggatggg atataccatc tacaccagcg caacccccgg accctatgag
     1081 ctgggccgcg ccgagcaggt ggtgcagcag atcatccgcc ccaccgggct ggtagacccg
     1141 caggttgaag tgcgccccac cgagggccag atcgacaacc tggtaggtga gatccgctgg
     1201 cgggtcgagc aggggcagcg caccctggtg acaaccctga ccaagcggat ggccgaaaaa
     1261 ttagccgatt acctgcttga gctgggcacc aaagtccatt acttacattc agagatcgat
     1321 acgctcgaac ggatcggcat tttgcgtgat ctgcgcctgg gggtgttcga cgtggtcgtc
     1381 gggatcaacc tgctgcggga aggattggac ctgcccgagg tctcgctggt ggcaatcctg
     1441 gatgccgata aagaaggctt tctgcgctca ggaacagcct taatccagac catcgggcga
     1501 gctgcccggc atgtgaacgg cagagtgatc atgtatgccg accggatgac cgattcgatg
     1561 aaatttgcga tcgatgagac caaccgccgg cgcgccattc aggaagccta caatgaggag
     1621 cacggaatcg agccggtcag catcatcaag gctgttcatg atctgaccga gcagatgtcg
     1681 gttcattccg tagccgagcc gcagggtggt tatagggtta aacaggatac tcccaaagct
     1741 gagatcacca gggttattgc cgagatggaa aagcagatga aggatgccgc ccgcaacctg
     1801 gaattcgaga aggcagcgat cctgcgggac cagatctacg agctgcgcac cattctggcg
     1861 gatgatccta aagtcaagcc ctgggaaaag tttaaactgt tatcgggaga ggaataaaga
     1921 aaagtcctgc tcagggactg atcaaggcac actcggggtg ccaagggctt gtttcagcca
     1981 gctgcgcact gcattcagat gttcgtgaaa atgctggtaa gagttgtggg cgatggaggt
     2041 catgaccgct tcgccgcgcc accagctaaa agccagcggg ttattgagca tgtcttcgga
     2101 gaggctctcc accagggcga ccaggcgcag gtgggtgctg cggaaatcct caaggacttt
     2161 ctcgaggggg gcatccttga atttctggta aatccggctg ttcagaagat tgatcactcc
     2221 ttcggggtcg tgctcaccga cttcaaaacc aggataatag cgctccggct gcttcccctc
     2281 cagggtagat tccagccagt acagctcgat tcgttcccag cagctcaggt gagccaggag
     2341 atccttgaag gatgatccgt tatccatcgc tgtatccagg atctgcgcct ccgaaagctt
     2401 caggataatc tgctcatac
//
LOCUS       DATMXK010000514         2861 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_62492, whole genome shotgun sequence.
ACCESSION   DATMXK010000514 DATMXK010000000
VERSION     DATMXK010000514.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2861)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2861)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2861
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62492"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            182..730
                     /locus_tag="VN363_09355"
     CDS             182..730
                     /locus_tag="VN363_09355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84762.1"
                     /translation="MTDHPDHAQFLPFHAINEFMRPDFRLGVVRSALQSVESALPEKR
                     SRLDQLTRKLVTVPGFRNSAKAPLALRARPAAEAFEKSAPLAAAIISAWAAARPELAS
                     RTHQLLSERGWEILPLEADRAKLPGFMIHWPKGDDFETLLAAYKTANPEDDTVDDEIS
                     LMVVWVGNRLPYDQDDDAGEAE"
     gene            727..1737
                     /locus_tag="VN363_09360"
     CDS             727..1737
                     /locus_tag="VN363_09360"
                     /inference="COORDINATES: protein motif:HMM:NF013046.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="histone deacetylase family protein"
                     /protein_id="HWQ84763.1"
                     /translation="MITVYAAQHALHATDQITVKGLPWPTEELPVRAELIRQAIIEAQ
                     LGEIIPPRDFGLAPIQAVHAPGFLDYLQTVHARNAALHAGRPSAVFPNTFATRPVRHT
                     SPSLDAQKGYYCFEVYTPVLEGTWAAAYWSAQCALTAARIVQTGAPAAYALCRPPGHH
                     AAVDLYGGFCFLNNAAIAARSLSARIAILDIDFHHGNGTQEIFYSDPDTLYCSLHADP
                     DVEYPYYWGGADETGAGAGTGTNLNLPLAIGTTDETYLPTLDQALERIQAFAPRYLLV
                     SLGLDIAAQDPVGGFRITLPGMAEIGRRIKRLGLPTVLVQEGGYHLDVLGSYAVAFLS
                     NF"
     gene            complement(1742..1909)
                     /locus_tag="VN363_09365"
     CDS             complement(1742..1909)
                     /locus_tag="VN363_09365"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84764.1"
                     /translation="MIDIDGDPNQKPSNRLSRSSLNSWIFGLVIFLIVEICALGVVVF
                     MWMVDSGILLR"
     gene            1961..2551
                     /locus_tag="VN363_09370"
     CDS             1961..2551
                     /locus_tag="VN363_09370"
                     /inference="COORDINATES: protein motif:HMM:TIGR00093.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0001522 - pseudouridine synthesis [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="pseudouridine synthase"
                     /protein_id="HWQ84765.1"
                     /translation="MPDRTILFHKPDEVLSQFTDTQGRRTLTDFIPIPDIYPAGRLDY
                     HSEGLLLLSSDGALIHRLTDPRFSHPKTYLAQVEGEVDPTAIERLNQEILLPGLQTRL
                     PRVSLIDPPDLPGRERPVRGYHPTAWLQVVLEEGKKHEVRRLTAAAGYPTLRLVRVAI
                     GELSLEGLPAGAWRDLTGEEEHNLRRALNLLEPGGK"
     gene            2557..2799
                     /locus_tag="VN363_09375"
     CDS             2557..2799
                     /locus_tag="VN363_09375"
                     /inference="COORDINATES: protein motif:HMM:NF012400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome b5 domain-containing protein"
                     /protein_id="HWQ84766.1"
                     /translation="MADLVFTSAQLRRYDGDDGPPYIAYKGIVYDVSRCTKWRGTLHE
                     GQHFPGQDLTGELKDAPHGEEVFRHPCVRRVGILIG"
BASE COUNT          567 a          887 c          789 g          618 t
ORIGIN      
        1 ggcctatcaa atggtctact cgctccgcca gctcgagctg ctggtggaga ccgggcagcg
       61 cggtcgcacc cacctgtacg ggcgcactcc cccggctgcc tgaggttgtc cggctttcgt
      121 gggtgatcag gttataattc cttcgatagt tgctcccaat cttccttcga aaggtacctg
      181 catgactgat caccctgatc acgctcaatt cttacccttt catgctatca atgagttcat
      241 gcgcccagac ttccggcttg gggtggtgcg ctcggcgctc caatccgtgg aatccgccct
      301 tcccgaaaag cgcagccgtc tggaccagct cacccgtaag ctggtaacag tacccggatt
      361 ccgcaacagt gccaaagcgc ccctggcgct gcgagccaga ccggctgccg aggctttcga
      421 gaaatccgca ccgctggcgg ccgcgatcat cagcgcctgg gctgctgccc ggccagagct
      481 tgccagccgc acccaccagc tgctcagcga gcgcggctgg gaaatcctgc ccctggaagc
      541 cgaccgggcg aaactgcccg gtttcatgat ccactggccg aaaggtgatg attttgaaac
      601 ccttctggca gcctataaaa ctgctaatcc tgaagacgac acggtggatg atgagatcag
      661 cctgatggtg gtgtgggtgg gcaaccgcct tccgtacgat caggatgacg acgccggcga
      721 agcggaatga tcaccgtcta tgccgctcag cacgccctgc atgctaccga ccagatcacg
      781 gttaaaggcc tgccctggcc cactgaggag cttccggtgc gggctgagtt gattcgccag
      841 gctattattg aagcccaatt gggcgaaatc attcctcccc gggattttgg tctcgctccc
      901 atccaggccg tccatgcccc tggattcctg gattatttac agaccgtcca cgcccggaat
      961 gccgccctgc atgctggcag gccttccgct gttttcccaa ataccttcgc tacccgcccg
     1021 gtgcgccaca cttcccccag cctggatgcg cagaagggtt attactgctt cgaggtttac
     1081 acccccgttc tggagggcac ctgggcggca gcttactgga gcgctcagtg cgccctgact
     1141 gccgcccgga ttgttcaaac tggcgctccc gccgcgtatg ctttgtgccg cccaccgggt
     1201 caccatgcgg ctgtcgatct gtatggtggt ttttgctttt tgaacaacgc tgccattgcc
     1261 gcccggtcac tttctgcccg gatagcgatc ctggatatcg attttcacca cggcaacggt
     1321 acccaggaga tcttttatag cgatcccgat acgctctact gctcgctgca tgccgatccg
     1381 gatgttgaat atccctatta ttggggcggc gcagacgaaa ccggggccgg agccgggacc
     1441 gggaccaatt taaatctgcc tctggccatc ggaaccactg atgaaaccta ccttcccacc
     1501 ctggatcaag ctttggaacg catccaggcc ttcgcgccgc gctatctgct ggtctccctc
     1561 ggtctggata tcgccgcgca ggatcccgtg ggtggtttcc ggatcaccct gccggggatg
     1621 gcggagatcg gccggcggat taagcgcctg ggtttgccca ctgtactcgt ccaggaaggt
     1681 ggttatcatc tggatgtgct gggaagttat gcggtcgcat tcttgagcaa tttctaaccg
     1741 gtcagcgcag gagaatgccg ctgtcgacca tccacatgaa caccacgacc cccagcgcgc
     1801 agatctcaac gatgagaaag ataaccaggc caaagatcca gctgttcagc gaggaccggc
     1861 tcagccggtt ggagggtttt tgattgggat caccatcgat atcgatcatc ttattctact
     1921 cgtggattaa cctgtaatct ggatcggaca acaattacca atgccggacc gcaccatctt
     1981 attccataag cccgacgaag tcctgtccca gttcaccgat actcagggcc gccgcaccct
     2041 gactgatttt atcccgatcc cggatatcta tccagccgga cggttggatt atcacagcga
     2101 aggtctgctg ctgctcagca gcgatggagc actgatccac cgcctgaccg acccgcggtt
     2161 ttcccatccc aaaacttacc tggcacaggt cgaaggcgaa gtcgatccca cggcaatcga
     2221 acgcctgaac caggagatct tgctgcccgg tctgcaaacc cgcctgccgc gcgtctcgct
     2281 gatcgatcca cccgatctgc ccggtcgcga gcgcccggtg cgcggttacc atccgactgc
     2341 ctggctgcag gtagtcctgg aggaaggcaa gaagcatgaa gtccgccgcc tgactgcggc
     2401 ggctggttat cccactttgc gcctggtgcg ggttgccatt ggtgagctga gcctggaagg
     2461 attgcccgcc ggcgcctggc gcgacctgac cggggaagag gaacacaatc tacgccgcgc
     2521 attaaattta ctcgaacctg gagggaaata aatcccatgg ctgatctggt ttttacttcg
     2581 gcccagctgc gccgctatga cggagatgac ggtccgccgt atatcgctta taaaggcatt
     2641 gtctatgatg tgagccggtg caccaaatgg cgcgggacgc tgcatgaagg gcagcatttc
     2701 cccggtcagg acctgaccgg cgagctgaaa gatgcacccc acggcgagga agttttccgc
     2761 catccctgtg ttcgccgcgt tggaatattg atcgggtgat aaaatattgc cccaacctga
     2821 ttaattaaat ctgtagacat aagagatgcc gacttgaacc g
//
LOCUS       DATMXK010000515         3161 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_32642, whole genome shotgun sequence.
ACCESSION   DATMXK010000515 DATMXK010000000
VERSION     DATMXK010000515.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3161)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3161)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3161
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_32642"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..870)
                     /locus_tag="VN363_09380"
     CDS             complement(<1..870)
                     /locus_tag="VN363_09380"
                     /inference="COORDINATES: protein motif:HMM:NF017812.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF917 domain-containing protein"
                     /protein_id="HWQ84767.1"
                     /translation="MIELTKQDLYDILYGCTILGTGGGGSLEKGLARIDEALANSKRF
                     RLVSFDEVPDEAWIAVPYMCGAISPSTPELEAQYAGLPELTEPMPYLAYKALEQYVGA
                     EFYGVMSTELGGGNTAEALYVGAWLDKFIIDADPAGRSVPEIQHSTFFINDLPVYPLA
                     CANMFGDVAIFPHVVNDLRAEALVRALAVASKNHIGVADHPAQARTLRNNVIKGAIRH
                     AWKLGTAWREARAAGQPVAALIADLGGGRVLFHGAVSHFGYQTVEGFTVGDVFIQGQG
                     SNQSNDYHIWFKNE"
     gene            complement(890..1936)
                     /locus_tag="VN363_09385"
     CDS             complement(890..1936)
                     /locus_tag="VN363_09385"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84768.1"
                     /translation="MSKQYEYELHKAAMILVQDMFRLQTGETVVITSDTGSDQRVVQA
                     VASAAHSAGAIPMVITLASPLGVGKAADPLLPVNALSAALLEADAWVEFNQQWLLYST
                     PFERAMAGNKRLRYMCLVEMTVDMMVRLIGRIDAPLLSTFMHKVTDLTQAANQMRITT
                     PVGNDLSFTMLPQERTVSCDDGLSDTPGMHFLGGQITFVPDFKSINGKLVFDGTIAPP
                     CGFLKEPVVLDVEQGRVVHISGGRQAAELRRWLEGFNDPNMFRLAHGCYGFNPGARLC
                     GNVLEDERIWGCTEWGMGYLSPVDAPPDGIQAISHMDGICLNSSVWLDGMQILNEGVV
                     IHPALKDLADQLIK"
     gene            complement(1965..2945)
                     /locus_tag="VN363_09390"
     CDS             complement(1965..2945)
                     /locus_tag="VN363_09390"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84769.1"
                     /translation="MTEPLVRITGLKKYFPVRSDALSAQKNWVKAVDGVDLEIMPGEV
                     IGLVGESGCGKTTLVNVLLRLEDPTEGILEFDGADITSLSQNTLRKDVRKNIQIVFQD
                     PFWSLNPRMLVRDVVGEPLSVHRKLPLLEKITEVERLLAMVGLPPDSMYKYPHEFSGG
                     QRQRIAIARALALEPRLVVLDEPTSSIDILSQAQILDLLIQLKQKLGLTYIIISHDLS
                     VVHYMSDRIIVMYLGKVVEQGPADVVFSSPVHPYTQALFNAIPSLEMSGLDELQTPAG
                     NVPSAIDPPSGCRFHTRCPFVMDICSQEEPQMLSVGERHTAACYLLNKTS"
     gene            complement(2942..>3161)
                     /locus_tag="VN363_09395"
     CDS             complement(2942..>3161)
                     /locus_tag="VN363_09395"
                     /inference="COORDINATES: protein motif:HMM:TIGR01727.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009898 - cytoplasmic side of plasma
                     membrane [Evidence IEA];
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0015440 - ABC-type peptide transporter
                     activity [Evidence IEA];
                     GO_process: GO:0015833 - peptide transport [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="oligopeptide/dipeptide ABC transporter
                     ATP-binding protein"
                     /protein_id="HWQ84770.1"
                     /translation="PYTQTLISAIPRDKSEALKFASIVIKPDAPNGCPYYSRCALAEE
                     ACAESEPMLEAIDGAQKVACFVAARRPS"
BASE COUNT          674 a          874 c          940 g          673 t
ORIGIN      
        1 ctcgttttta aaccagatat gataatcgtt ggactggttt gagccctgac cctgaatgaa
       61 tacgtcaccg acggtgaagc cttctaccgt ctggtagccg aaatgggaga cagcgccgtg
      121 gaagaggacg cgcccaccgc ccaaatcagc gataagcgca gcgaccggct gaccggcagc
      181 ccgcgcctcg cgccaggctg tccccagctt ccaggcgtgg cggatagcgc ctttaatgac
      241 attgttgcgc agggtgcgcg cctgggcggg gtggtccgcc acaccgatgt ggttcttgct
      301 ggcgacagcc agggcgcgca ccaatgcttc ggcgcgcagg tcgtttacca catgggggaa
      361 gattgccacg tcgccgaaca tattggcgca ggccaacggg tagacaggca gatcattgat
      421 gaaaaatgtg gagtgctgga tctccggcac agagcgtccg gcggggtctg cgtcaatgat
      481 gaatttatcc agccaggcgc ctacgtacag ggcttcggca gtgttaccgc cgcccagctc
      541 ggtggacatg acgccgtaaa attcggctcc cacgtactgc tccagggctt tataggcgag
      601 gtaaggcatc ggctcggtca gctcgggaag gccagcatac tgtgcttcca gctcgggtgt
      661 ggagggggaa attgcgccgc acatatacgg cacagcaatc caggcctcgt ccggaacttc
      721 atcgaagctc accagccgga aacgtttgct attggcaagc gcttcatcga tacgggcgag
      781 gcccttctct agagagcctc caccgccagt gccaaggatg gtgcagccgt aaaggatatc
      841 gtataggtct tgtttggtga gctcgatcat agaaatttcc tcacagaagt tatttgatta
      901 actggtcagc caggtctttg agcgcggggt ggataaccac gccttcgtta aggatttgca
      961 tgccatccag ccagactgag gaattaagac agatgccgtc catatgggat atggcctgga
     1021 tgccgtccgg aggagcgtcc accgggctga ggtagcccat gccccactcg gtgcagcccc
     1081 agatgcgctc atcttcgagt acattgccgc acagccgggc gcccgggttg aagccatagc
     1141 agccatgtgc caggcggaac atattggggt cgttgaagcc ttccagccag cggcgcagct
     1201 cggcagcctg ccgtccgccg ctgatgtgca ccacgcggcc ttgttctaca tcgaggacga
     1261 cgggctcttt gagaaagcca cagggtgggg cgatggtgcc atcgaagacc agcttgccgt
     1321 ttatgctttt aaaatcgggg acgaaggtta tctgcccacc caggaagtgc atgccgggtg
     1381 tgtcgcttaa gccatcatcg catgagacgg tgcgttcctg cggcagcatg gtaaagctca
     1441 gatcgtttcc caccggtgtg gtgatacgca tctggttggc tgcctgggtc agatcagtca
     1501 ctttgtgcat gaaggttgag agcagtggag catcgatccg cccgatcagg cgcaccatca
     1561 tatcgactgt catctccacc aggcacatat agcgtaagcg tttgttcccg gccatggccc
     1621 gctcaaaggg ggtggaatag agcagccact gttgattgaa ctctacccag gcatccgcct
     1681 ctaaaagggc ggcagataag gcatttaccg ggagcaatgg gtcggcggct ttacctaccc
     1741 cgagaggcga ggccagggtg atcaccatcg ggatggcgcc ggcggagtgg gcggcgctgg
     1801 ctaccgcctg taccacgcgc tgatcgctgc ctgtatcgga tgtaatcacg accgtctcac
     1861 cggtttgcag gcgaaacata tcctggacca ggatcatggc agctttgtga agctcgtatt
     1921 catattgttt cgacatcaac ggctccttta attaaccagc gaatttagct tgttttgttc
     1981 agtagatagc aggctgcagt atgccgctca cctacggaca gcatctgcgg ttcctcctgc
     2041 gagcaaatat ccatcacgaa tggacagcgg gtatggaaac ggcagcctga aggtgggtcg
     2101 atggcactgg gcacatttcc ggcgggggtt tgcagctcat ccagcccgct catctccagc
     2161 gatggaatag cattgaacaa cgcctgggta tagggatgca ccggggagct gaaaaccaca
     2221 tctgctggcc cttgctccac gacctttccc aggtacatca caatgatgcg gtcggacata
     2281 taatgcacaa cgctcaggtc atgtgagatg atgatgtaag tgagacccag tttctgcttc
     2341 agctggataa gcaaatccaa tatctgggcc tgggaaagaa tatcgatcga agaggttggc
     2401 tcgtccagga ccaccagcct gggctcaaga gccagggcgc gggcgatggc aatccgctgg
     2461 cgctgtccgc cggagaattc gtgggggtat ttgtacatgc tgtctggcgg aagacccacc
     2521 atagccagca gtctttcgac ttcggtaatc ttttcgagca gcggcagctt gcggtgcacg
     2581 ctgagcggct caccaaccac atcccgcacc aacatccgtg gattcaggga ccagaaaggg
     2641 tcctggaaga cgatctggat gtttttgcgg acatctttgc gcagggtgtt ttgggataag
     2701 ctggtgatat cggcgccatc gaattccaat atgccctctg taggatcttc caaccgcagc
     2761 agcacgttga cgagcgtggt cttgccgcag cccgattcgc ccaccaggcc gatcacctcg
     2821 cccggcatga tctcgagatc gaccccgtct acggctttta cccagttctt ctgagcgctg
     2881 agggcatccg agcggaccgg gaaatatttt ttcagcccgg tgatcctgac cagcggctca
     2941 gtcatgacgg cctcctggcg gcgacaaagc aggctacttt ctgggctcca tcaatcgctt
     3001 cgagcatggg ctcgctttcg gcgcaggctt cttcagccag ggcacagcgg gaataataag
     3061 ggcagccatt tggagcatcc ggcttgatca cgatgctggc aaatttcagc gcctccgatt
     3121 tatcacgggg gatggctgag atcagtgtct gagtataagg a
//
LOCUS       DATMXK010000516         3112 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_141751, whole genome shotgun sequence.
ACCESSION   DATMXK010000516 DATMXK010000000
VERSION     DATMXK010000516.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3112)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3112)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3112
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_141751"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            74..1240
                     /locus_tag="VN363_09400"
     CDS             74..1240
                     /locus_tag="VN363_09400"
                     /inference="COORDINATES: protein motif:HMM:NF024808.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="extracellular solute-binding protein"
                     /protein_id="HWQ84771.1"
                     /translation="MKLSPITYKLSIGFLVVALLLTACTPAAPAVTPPADTPAPGEAA
                     TQPAAPGADTPAGDSPAVQAPGPLTILEWSGYELPEFWQPFADQYPDVKVDFSFFADD
                     AEAFAKLQTGFEVDVVHPCFSWLGQYVEAGLIQPIDTSRLSNWSGITPELASLGEYDG
                     KQYFVPWDWGYETTLVRTDKVTEIPDSYADLWDPQYAGHVSIYDAAESVWVVTALALG
                     LDPYNTTLEQQEQIKQKMIELKPNLLNYWTDYSEIVQLVASGDVWIASNAWNDAYVLL
                     QEENIPVEYTTPKEGRLGYVCGYVITSQTENLDLVYAYIDAAIAPQSMANLSNMYGYG
                     AANAEALPLTDPEFVAVMELDNPDILNRTYFYKPIDNAMRDLITGIWSEVKAAP"
     gene            1253..2104
                     /locus_tag="VN363_09405"
     CDS             1253..2104
                     /locus_tag="VN363_09405"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84772.1"
                     /translation="MKNHLRIATLMTPPALVLLVLFVIPLVVMAVFSFRAGTFGAQRE
                     IFTLDHYRDYLDNPVFHNLLLRSTWIALQTALFSVLLAYPLAYYLAFHAGAKRFSLLT
                     LLLVPAWTSYLLRVLAWKIMLSSGGVFNTVMMALGLVDEAQPILLYSRGAVIVTLVYV
                     WIPFVALPIYAALQRIDSSLLEAAADLGSPPWRSFLKVTVPLSLPGVLAGFFIVFIPS
                     LGEWVTPQLVGGVTGSMYGNVIQDQFVRGLNWPVGALMSLVMLVFVLLFLLVFSRVGK
                     LSDLAGV"
     gene            2107..2922
                     /locus_tag="VN363_09410"
     CDS             2107..2922
                     /locus_tag="VN363_09410"
                     /EC_number="7.-.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_component: GO:0055052 - ATP-binding cassette (ABC)
                     transporter complex, substrate-binding subunit-containing
                     [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84773.1"
                     /translation="MRRDPDPRHPWLASYYWLLAFFLYLPILFLLIFSFNDSPVLVFP
                     LKGFTLRWYQGLLGAEELIASVWNSLGLGLGSSLIATVLGTMAGIAITRFDFPGKQAF
                     IALSALPLVIPYVVLGVGLLILFDSLGIELSLWTVGLGHIVINVPYVMLIIAARLAGF
                     DPSLEEAAMDLGSNYWRTLRRVTLPITFPALLSAFLMSFTTSFDEFALSFFLIGNETT
                     LPVYLYSQLRFPSRLPLIVSLAAVIMVVSVLVVVLSEWLRRFGQPDQPVKVEL"
BASE COUNT          537 a          898 c          887 g          790 t
ORIGIN      
        1 gtcagatcgc gtggatattt tcaattaacc tggactgccg ggctgtttac ctcgctaaag
       61 gagaaaacct gaaatgaaat tgtcccccat aacctataag ctgtcgattg gtttcctggt
      121 agtagcgctt ttgctcactg cctgtacgcc agccgcgcct gcagttacgc ctccggctga
      181 tacccctgcg ccgggggaag cagccacgca gcctgcggcg cctggggctg atacacctgc
      241 aggtgactct ccagcagtcc aggcgccagg accgctcact attctggaat ggtccggcta
      301 tgagctgccc gaattctggc agccgtttgc cgaccagtat ccggatgtga aggttgattt
      361 cagctttttc gccgacgatg cagaagcgtt tgcgaagctg caaaccggtt ttgaggtcga
      421 tgtggtccat ccctgttttt cctggctggg acagtacgtg gaggccgggc tgatccagcc
      481 catcgatacc agccgcctta gcaactggtc tgggattacc cccgagctgg ccagtctggg
      541 tgaatacgat ggcaagcagt actttgtgcc ctgggattgg ggttacgaga ccaccctggt
      601 gcgcaccgac aaggtcacag aaatacctga ttcctatgcc gatctgtggg acccgcagta
      661 tgccgggcat gtttcgattt acgatgccgc cgaatcggtc tgggtggtca cagccttggc
      721 tctgggactg gacccttaca atacaaccct cgagcagcaa gaacagatca agcagaagat
      781 gatcgagctg aagccaaatt tgctcaacta ctggacggat tattccgaga tcgtgcagct
      841 ggtcgccagt ggggatgtgt ggatcgcttc caatgcctgg aatgatgcct acgtgctctt
      901 gcaggaggag aatatacccg tcgaatatac gactccaaaa gaaggccgcc tggggtatgt
      961 gtgcggctat gtgatcacca gccagaccga aaacctggat ctggtttacg cctatatcga
     1021 tgccgcgatt gctccccaat ccatggcgaa tttatctaac atgtacggat acggggcggc
     1081 caatgcagaa gccttgccgc tgaccgatcc ggaattcgtc gcggtcatgg agctggataa
     1141 tccggatatt ctcaaccgca cctatttcta caagccgatc gacaacgcca tgcgcgacct
     1201 gatcaccggg atctggtctg aagtcaaagc tgccccataa acgctcagcc ggatgaaaaa
     1261 ccacctgcgc atagcaaccc tgatgacccc gccggctctg gtcctgctgg tgctgtttgt
     1321 gatcccactg gtggtcatgg cagtcttcag cttccgggcc ggtaccttcg gggcgcagcg
     1381 cgaaattttt accctggacc attatcgcga ttatctggac aatccggtct tccataattt
     1441 actcctgcgt tcgacctgga ttgccctgca aacagcgctt ttctcagtcc ttttggccta
     1501 tcccctggct tattacctgg cattccatgc cggggcgaag cgtttcagcc tgctcaccct
     1561 gctgctcgtc ccggcctgga ccagctattt gctgcgagtt ttagcctgga agattatgct
     1621 ttcttcaggc ggggttttta atacggtcat gatggcgctc ggtctggtgg acgaggccca
     1681 accgatcctg ttatattcgc gcggggcggt gatcgtcacc ctggtgtatg tgtggattcc
     1741 ctttgtagcg ctgccaatct acgccgcctt gcagcggatt gattcgagcc tgctggaagc
     1801 tgctgctgac ctgggcagcc caccctggcg cagctttctg aaggttaccg ttcctctcag
     1861 tctgccgggt gtgctggcag gtttcttcat cgtgtttatc ccctccctgg gtgagtgggt
     1921 gacccctcag ctggtgggtg gggtgaccgg ctccatgtac ggcaatgtga tccaggacca
     1981 gttcgtgcgc ggccttaact ggccggtcgg cgctttgatg agcctggtaa tgttggtgtt
     2041 cgtgctgctc ttcctgctgg tcttcagccg ggtgggtaag ctcagcgatc tggcgggagt
     2101 gtagccgtgc gccgcgatcc agaccctcgc cacccctggc tggcatccta ctactggctg
     2161 ctggctttct tcctctatct gccgatcctg tttttactga ttttttcctt caatgactcg
     2221 ccggtgctgg tctttccgct gaaaggcttt accctgcgct ggtaccaggg tctgctggga
     2281 gcggaggagc tgatcgcctc ggtctggaac agtctgggtc tgggattggg ctcgtctttg
     2341 atcgcaactg tcctgggcac gatggcgggc atcgccatta ctcgcttcga tttccccggc
     2401 aagcaggctt tcatcgccct gtcggcgctg cccctggtga tcccttatgt tgtgcttggg
     2461 gtgggtctgc tgatcctgtt cgacagcctg gggatcgagc tgtctctgtg gacggtcggg
     2521 ctgggccata ttgtgattaa tgtgccgtat gtgatgctga tcatagctgc ccgcctggcc
     2581 ggctttgacc ccagcctgga agaagccgcc atggatctgg gctcgaatta ctggcgcacc
     2641 ctgcgccggg tgaccctgcc aattactttc cccgcccttt tatccgcctt cctgatgtcg
     2701 ttcaccacct cgtttgacga gtttgccctg tcttttttct tgattggcaa cgagacaacc
     2761 ctgccagtgt acctctattc ccagctgcgc tttcccagcc gcctgccgct gatcgtcagc
     2821 ctggctgcgg tgatcatggt cgtctctgtg ctggtggtgg tgctctccga gtggctgcgc
     2881 cggtttggcc agccagacca accggttaaa gtcgagctct gattcgaaat cagccaggtc
     2941 tcgtgtgcca gtttcggtac ctggcgcatg ggattgttta attcaactcc tgctgtatgc
     3001 caacgattct atcgcgccga cccggcgcgc gccgacccgg cgcgcagcgc cagccccatt
     3061 ttgctggggc tggcgctggg gatttgtaaa gcgctggtga tcacccaggg tg
//
LOCUS       DATMXK010000517         2281 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_19717, whole genome shotgun sequence.
ACCESSION   DATMXK010000517 DATMXK010000000
VERSION     DATMXK010000517.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2281)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2281)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2281
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_19717"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..697)
                     /locus_tag="VN363_09415"
     CDS             complement(<1..697)
                     /locus_tag="VN363_09415"
                     /inference="COORDINATES: protein motif:HMM:NF016013.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF389 domain-containing protein"
                     /protein_id="HWQ84774.1"
                     /translation="MNLSQPDPIPDDPNRLPPARRRRALRLLAPLDADERASFSAELA
                     LRISPSFDFFLFSVISAFIMGIGLLLSAPALLVLGAIFAPPMAPVIGASLGTVLGSFR
                     FFFRSLFGIILASLLVFSVGYGAGRLALSGPANDFGLASIYAQLNWTNFLLLALAAIL
                     TAAGIARSNRGPLLASVGLAYELYLPLVAAGIGFGSGVPHLWPDGLVVFAVHLAWATL
                     LGAITLGILGFRPL"
     gene            complement(694..1164)
                     /locus_tag="VN363_09420"
     CDS             complement(694..1164)
                     /locus_tag="VN363_09420"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84775.1"
                     /translation="MITLNIPGRGQLNLEHLVCDVNGTLALDGQLIEGIARQLNRLRD
                     RLELHLLTADTHGRQSILDHQIGVQAVRINPGDEVEQKTAYLQTLGAERAVAIGQGAN
                     DAGMLAAAGLGICVLSREGTAVEALLAADLVVPDIFSALDLIEKPLRIVASLRK"
     gene            1346..>2281
                     /locus_tag="VN363_09425"
     CDS             1346..>2281
                     /locus_tag="VN363_09425"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84776.1"
                     /translation="MQNIKSSGYHRIGLWVILTWLLMGCSTATSSTSSPTPPPISSET
                     ATQPQITPTQTSVPTDTPAPGRVILLAAEGSQPGLQALLGELSQAENLSFNAQPSITP
                     SEIGPEVRLVLATSPDPGIASLAAGAPMTQFLAIGIPGLEPAANLSVLSGAGQHPDKI
                     GFLAGYLAAAITEHWRVGMLSISDDLAGQTAWQGFNNGVVYFCGLCRPAYPPYVQYPV
                     FAAVPANAVLTEQQAAVDSLVSQSVNTVFVAGSLMNPELAAYLASNEIHVISDALPPL
                     EDDPNWVATVSPGDISAAIRQLWPGIMAGNPGGQVE"
BASE COUNT          543 a          628 c          673 g          437 t
ORIGIN      
        1 tcagcggccg gaaaccgaga attcccagag ttattgcacc caatagagta gcccaggcca
       61 ggtgaacggc aaagaccacc agaccatccg gccataaatg ggggacgccg ctgccaaatc
      121 caattccagc agcaaccagt ggtaggtaca gttcataagc caggcctaca ctggccagaa
      181 gtggaccgcg gttcgagcgg gcgattccag ccgcggtcag gatagctgcc agggccagta
      241 aaaggaaatt tgtccagttg agctgggcgt agatggatgc cagcccaaaa tcattggccg
      301 gtccgcttag cgccagcctg ccggcgccat aaccgacgga aaagacaagc aaacttgcca
      361 ggatgatccc gaacaggctg cggaagaaga accggaagga acccaggaca gtccccaggg
      421 aagcgccgat cacaggcgcc atcggtggcg cgaaaattgc tcccaggacc aggagcgcag
      481 gtgcgctgag cagcaggcca atccccataa tgaaagctga aatcactgag aaaaggaaga
      541 aatcgaagga aggcgaaatt cttaaggcca gttcggccga gaaagaagcg cgttcatcgg
      601 catccagtgg tgctagcagg cgcaaagcgc gccggcggcg agcaggtgga aggcggtttg
      661 gatcatcagg gatgggatcg ggttgagaga gattcatttt cgcaagctgg caactattct
      721 taatggtttt tcgatcaaat ccagagcaga aaaaatatcg ggtactacca ggtcggcagc
      781 gaggagtgcc tctaccgctg tcccctcccg ggagaggacg cagatcccca ggccagcagc
      841 agccagcatt cctgcgtcat tcgctccctg cccgatcgcg actgcccgct ccgcacccag
      901 cgtctggagg taggcggtct tttgttccac ctcatccccc ggattaatcc ggaccgcctg
      961 cacgccgatc tggtgatcaa ggatactctg gcgtccatgg gtatctgcag tgaggagatg
     1021 cagctccagc cggtcacgca agcggtttaa ttggcgagcg atcccttcaa tcaactgtcc
     1081 atcaagagcc agggtgccat taacatcgca caccaggtgc tcaaggttta gctggccgcg
     1141 cccagggatg ttgagggtga tcatgctggg tattatacaa gatttatgcc agaaactcgg
     1201 ccaatgcgat cgctctcgcc gctggcagga agccgtctcc gtgggcaatc tggtaataaa
     1261 gcgtaccagg gggatatagt aaaataaggc tcaatattaa gcaaagggtg cgtttatact
     1321 acagcagccg tccgggagag catttttgca gaatataaaa tcatcgggtt accaccgaat
     1381 agggttatgg gtcattttga cctggctgct gatggggtgc agcacagcga cttcatccac
     1441 cagcagtcct acgccacctc caatcagcag tgagaccgcc acccagccgc aaattacgcc
     1501 cacccagacc agtgttccga cagatacgcc agcacccggg agggtaatcc tgctggcggc
     1561 ggagggcagc cagcccggtt tgcaagcgct gttgggggaa ttgagccagg cagaaaacct
     1621 gtcattcaat gcccagccat cgatcacgcc atctgaaatc ggaccggaag tgcgtctggt
     1681 tttagcaacc tctcctgacc cgggaattgc cagcctggcg gcaggcgctc ccatgaccca
     1741 atttctggcg attggcatcc cgggcctgga acccgcggcg aacctgagtg ttctgagtgg
     1801 tgcaggccag cacccggaca agattggttt tctggcaggt tacctggccg cggctattac
     1861 agagcactgg cgggttggta tgttgagcat cagcgatgac ctggccgggc agacagcctg
     1921 gcagggattt aataacggcg tcgtctattt ttgcggatta tgccgtccag cctacccgcc
     1981 gtatgtgcag tacccggtat ttgccgctgt acctgccaat gcggtgctga cggaacaaca
     2041 ggcagcggtg gacagcctgg tttcccaatc tgtgaatacg gtttttgtgg ccgggagttt
     2101 gatgaacccg gagctggctg cctacctggc gagcaatgaa atccacgtga tcagcgatgc
     2161 tcttccgccg ttggaggatg atccgaattg ggtagcgact gtcagccccg gagatatttc
     2221 cgccgcgatt cgccaattat ggccgggcat aatggcaggt aaccccggtg gacaggtaga
     2281 a
//
LOCUS       DATMXK010000518         4575 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_57334, whole genome shotgun sequence.
ACCESSION   DATMXK010000518 DATMXK010000000
VERSION     DATMXK010000518.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4575)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4575)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4575
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_57334"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..4063
                     /locus_tag="VN363_09430"
     CDS             <1..4063
                     /locus_tag="VN363_09430"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84777.1"
                     /translation="RQVLGLPDQSPIHPKEQRASRNRQFLKSLLSGLEINWTEESGER
                     LRKQVRAAFNHVGSVGLQSSHYNAAWLDADGTLLNMPAAGTPQAKGLNQFVANQFAVY
                     THMPPGAPIAQNPPDFDTLIDFHQALSSLNSYPELLRALGLVFDFELPASFVATTSIN
                     VPRRLSVIDVPHHSWAIPTQAPPNIAPLETAYLYFSVGSPPDAWQIFTTAPGLLGGGM
                     TDLEVFGLLNLDPTRFGLAQVDLESGMFKNTLLAESWQADRPGPDPSDHPEVFDETTT
                     LPALRSGGFSLFADGRALSLLHSFQENKQFNVDLTNSAPLNRPFFAEDLTQGFRIDIW
                     DSLTNAWHSLHRRNGVYQIEDQEFTTTDEEGFTELAAGKAAPDPDNPTPEDLYLNESV
                     ARWVGWSLSAPFPGKALSADTDPALALVEDPEHPQNEPATPFKMTTKYKAFPNSLPAL
                     RFGRRYRLRARAVDLCGNSLGHSDPLAHMLAFLGGLPREPEGFAYLRYEPVIAPFVVL
                     RDERGVTGPGSQLERLVIRTFNADPSKDNDPADLTASDRFISPPSTSVEVAERLGMFD
                     DSNGKLVNSAAMYNLIGVRDQGKYTHVQVEVAGQLKEFPLEAGETIASLPFLPDVLAR
                     GAALRNLPGTPDGSLAIVAPAPGGPVPIPYPVLVDANPRPGSATLVSFGEQGDWQKLQ
                     PFRLALADGEGAPDWDPQGRVLTVHLPKGTQALTPLSSYLLPDDLKRMGVWQWLREYI
                     DRITVQDPTLPVLDPQVDSEQIAHILQRAIEGGHWMITPPTLLSLVHAVQQPVGTPQF
                     TAIAAQHEPYGSENIYGQWDELLAPDPEVLQTTPESNLSAGTETTLITAWRKPEATDA
                     FLLGGLHVHAASTGKVDLLAEWDDPYDDLTGPRLEGVDYSQKNTALGDEILIPTTQEG
                     YLITGDDTPNYRQQAYYDADHDLLCFARHGDLLGNLKSGVTIYGDTAPRHALIDTRHH
                     TVFYIARATSRYQEYFAQDQNLDFTRSSLPVKVEVPASARPAAPLVDYIVPTFGWQRQ
                     TQTNLKRSVRFGGGLRVYLERPWFSSGAGELLGVALYDYSNGSLTDRELWKGYVTQWG
                     ADPIWASPGLGLVPAAYHFPNALTSEPGLSLPGKAPGRVQVAGFTVDFDFDTQKWFAD
                     LTVDIQSEAYTPFIRLAFVRYQPFALPDAKLSTVILADYAQLTPERSAMITADPYHPR
                     RLRLTVSGPAPSGPVPEITGRQPNQPVTVPTRVEVTLQRRNPAVLSDLGWEDAPAGTA
                     SITPLPIPVTPQTSLVRWTGTVNFAQLPEAGEYRVLIREFEYLPANYVKTVQVGRRIR
                     REQPRRLIYAEAIEIDATLIAGPSGSTGTVLVG"
     gene            complement(4157..>4575)
                     /locus_tag="VN363_09435"
     CDS             complement(4157..>4575)
                     /locus_tag="VN363_09435"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ84778.1"
                     /translation="YAASVVSPSDPAASIVEVTETGEGMVNQGCSCETALWPTLSITE
                     NCAACGPCARYCPSGALSTRVVGSTFIHFFTPGVCVACGLCAQVCHSGALERSYAPEP
                     DPFEERVMAVRPIKTCRKCGSPALDALNGLCYWMRQ"
BASE COUNT          922 a         1379 c         1341 g          933 t
ORIGIN      
        1 gagacaggtt ttggggctgc cggaccagtc ccccatccat cctaaagaac agcgagcttc
       61 ccggaatcgc cagtttctga agagcctgtt atcaggcctg gaaattaact ggactgagga
      121 aagcggggag cgtttgcgca agcaggtccg ggcagctttc aaccatgtag gctcggtcgg
      181 gctgcagtcc agccattaca acgcagcctg gctggatgca gatggcacgc tgctgaatat
      241 gccggcggca ggcaccccac aggcaaaagg gttgaaccag tttgtcgcca accagtttgc
      301 ggtgtatacc cacatgccgc cgggtgcgcc gattgctcaa aatcctccag atttcgatac
      361 cctgatcgat ttccaccagg cactcagctc gctcaactcc tatcccgagc tgctccgggc
      421 tttgggtctg gtgttcgact tcgagctgcc ggcgagcttt gtggccacaa catcgattaa
      481 tgtccccagg aggctgtcgg tgatcgatgt tccccaccat agctgggcga tccccactca
      541 agcgccgccc aatattgcac cgctggagac ggcttacctg tatttcagtg ttggaagccc
      601 gccggatgcc tggcagattt tcacaactgc ccccgggctg ctgggcggcg gaatgaccga
      661 cctggaggtc tttggcttac taaacctgga cccgactcgc ttcgggttag ctcaagttga
      721 cctggaaagt ggaatgttca aaaacacttt gctggccgaa agctggcagg cggaccgccc
      781 cgggccagac ccttcagacc atccggaagt gtttgatgag acgaccacac ttccggcgct
      841 gcggtcgggt ggtttttcgt tattcgccga tggacgcgcc ttgagcttgc tgcactcctt
      901 ccaggaaaat aagcagttta atgtggatct aacgaacagc gcaccgctca accggccctt
      961 ctttgcggag gatcttaccc aggggtttcg catcgatatc tgggattcgc taaccaatgc
     1021 ctggcattcg ctgcaccgcc ggaatggggt ctaccagatt gaggatcagg aatttactac
     1081 caccgatgaa gaaggtttta ccgaactggc agccggaaaa gctgctcccg atcctgataa
     1141 ccccacccct gaggacctgt atctgaacga atcggttgcc cgctgggtag gctggagctt
     1201 gagtgcgccc ttccccggca aggcgctctc ggcagatacg gacccggcgc ttgccctggt
     1261 tgaggatccc gagcatccac aaaacgagcc ggcaacaccg tttaaaatga ccacgaaata
     1321 caaagctttc cccaattctt taccagcgct gcgtttcggg cggcgctacc gattgcgggc
     1381 acgggcagtg gatctgtgtg gtaacagcct ggggcattcg gatccactgg cgcacatgct
     1441 ggctttcctc ggtgggctgc cacgtgagcc ggaaggattt gcctatctgc gctacgagcc
     1501 ggttattgcc ccatttgtgg tgctccgcga tgaaagaggc gtgaccggtc ccggctcaca
     1561 gctcgagcgc ctggtcatac gcaccttcaa tgctgatcct tccaaagata acgatccggc
     1621 ggacctgacc gcttccgacc gcttcatttc acctcccagc accagcgtgg aggtggctga
     1681 gagattgggc atgttcgacg actcgaacgg caagctggtg aacagcgccg cgatgtacaa
     1741 tttgatcggc gtgcgcgacc agggcaagta cactcatgtc caggtcgagg ttgccgggca
     1801 gcttaaggaa tttccattgg aggcgggcga aaccatcgct agtctgccct ttttaccgga
     1861 tgtgctggcc cgcggagcgg cgctgcgcaa tctgcccgga actcctgatg gcagcctggc
     1921 gattgttgcg ccagctcccg gtggacctgt tccaattcct tatccggttc tggttgatgc
     1981 aaaccctcgc cccggctcag cgactctggt gagttttggc gagcagggag actggcagaa
     2041 gctgcagccc ttccggctgg cgctggcaga tggggaggga gccccggatt gggacccaca
     2101 gggccgggta ctgacggtcc acctgcctaa aggcacccag gcgcttaccc ccctgagcag
     2161 ctacctcctg cccgatgacc tcaagcggat gggggtctgg cagtggctgc gcgagtatat
     2221 cgaccggatc actgtacagg atcctacgct gcctgtgctc gatccccagg tcgatagtga
     2281 gcagatcgcc cacattttgc agcgtgccat tgagggcggg cattggatga ttaccccgcc
     2341 aacgcttttg agccttgtcc atgctgtgca gcagccggtt ggaacacctc aattcacggc
     2401 cattgccgcc cagcatgagc cgtatgggag cgagaatatt tatggtcagt gggacgagct
     2461 gctcgcaccg gatccagagg tgctgcaaac tacccctgag agcaatctgt ctgctggaac
     2521 cgagacgaca ttaattaccg cctggcgtaa gcctgaagcg acggatgcct tcttgctggg
     2581 cgggctgcat gttcacgccg ccagtactgg caaagttgat ctgctggctg aatgggatga
     2641 cccttacgat gatctgaccg ggccgcgcct ggagggagtg gattactcgc agaagaatac
     2701 tgccctgggg gatgaaatct tgatccccac gactcaagaa ggctacttga tcaccgggga
     2761 cgataccccg aattaccgcc agcaggcgta ttacgacgcc gaccacgact tgttgtgttt
     2821 tgcccgccac ggcgacctgc tgggaaacct gaaaagcggc gtgacgatct acggggacac
     2881 tgcccctcgc cacgcgctga ttgacacccg ccaccatacg gtgttctata tcgcccgggc
     2941 aacctcgcgc taccaggaat attttgccca ggatcaaaac ctggacttta ctcgcagcag
     3001 cctccccgtg aaagttgaag tgccggcttc agcccgtccg gcagctccgc tggtcgatta
     3061 catcgtgccg acttttggct ggcagcgcca gacccagacg aacctgaaac gcagcgtacg
     3121 cttcggcggt gggctgcgag tttacctcga gcgtccctgg ttttcctccg gggcgggtga
     3181 gctgcttggc gtggcattat atgattacag caatggctcg cttaccgacc gcgagctgtg
     3241 gaaagggtat gttacccagt ggggcgccga cccgatctgg gcatcgccag gacttggcct
     3301 ggttccagca gcctatcact tcccgaacgc cctgaccagc gagccaggcc tgagcctgcc
     3361 aggcaaagcg cctggccgcg ttcaggtagc gggcttcacc gtggattttg atttcgatac
     3421 ccagaagtgg tttgccgacc tcacggtgga tatccagagc gaagcctaca ccccattcat
     3481 ccgcctggca tttgtccgct accagccatt tgcactgcca gatgcaaagc tttcaacggt
     3541 aattctggca gattatgccc agcttacccc cgaacgctca gccatgatca cggcagaccc
     3601 ctatcaccca cgccgcctgc gccttacagt gagtggtcct gcccccagcg ggccggtccc
     3661 ggaaatcacc ggacggcagc ccaatcagcc ggtgaccgta ccgacccggg tcgaggtgac
     3721 tctgcagcgc cgcaacccgg ctgtattgag tgatctgggt tgggaagatg cccctgccgg
     3781 gacggcttcg atcaccccac tgcctatacc cgtaacccct caaaccagcc tcgtccgctg
     3841 gaccgggacg gtgaacttcg ctcagctgcc tgaggctggc gagtaccggg tgctgatccg
     3901 ggaattcgag tacctccctg ccaattatgt gaaaacggtg caggtggggc ggcgcatccg
     3961 ccgcgagcag ccccggcgct tgatttatgc cgaggcgatc gagatcgacg cgactctgat
     4021 cgcgggaccc tccggttcaa ccgggacggt attggtaggt taaccctatt cttgtctgga
     4081 taggcaggca gatcaatctc gctaacgtcc gatcaagaag ttgcgggcgt tagccatcag
     4141 tgagcgcatg ggcggctcat tggcgcatcc agtaacacag cccattcagc gcatccaggg
     4201 cagggctacc gcacttgcgg caggtcttaa tcgggcgtac agccataacg cgttcttcga
     4261 atggatcagg ctcaggggca tagctgcgct ccagtgctcc agaatggcac acctgggcgc
     4321 ataagccgca cgccacgcaa acgccgggag tgaaaaaatg gataaatgtg ctgccaacaa
     4381 cccgtgtgga taaagcgccg gaagggcagt agcgggcgca ggggccgcag gcagcgcagt
     4441 tctcggtgat gctaagcgtg ggccagaggg cggtttcaca gctgcatccc tggttgacca
     4501 tcccctcgcc ggtttctgtc acctcgacta tggacgctgc cggatcagag ggagagacaa
     4561 cgctggctgc ataga
//
LOCUS       DATMXK010000519         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_97522, whole genome shotgun sequence.
ACCESSION   DATMXK010000519 DATMXK010000000
VERSION     DATMXK010000519.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97522"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1283
                     /locus_tag="VN363_09440"
     CDS             <1..1283
                     /locus_tag="VN363_09440"
                     /inference="COORDINATES: protein motif:HMM:NF012618.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84779.1"
                     /translation="SATLQPTPHLLPDLTLYRVEITLEADDPCADPDAGLGVRVWLRN
                     SGLADSAPFSIQVNQQDQAIESALSPGEQASLWFAGYESLNEIRLDVYSQVMESDETN
                     NHEIRQLLLPTLPAGCQPQAQPELESQDPLYSLEGHTGKVWSVAFSPDGGYLASVGID
                     NSLRLWRVDQGFLLRTMQGHPYPIRSLAFNHRGTEIATGSYDGVVRIWQVSNGMLQRS
                     LTGHAGRVLALAYSPNDRSLASTAEDFTVRLWRVQSGVLLNLIDEGMEQIESVEFLPD
                     GSALVWGEASGDLRMWNISDNRWQLHLKGLGLPVRSIGLTADGALLAAGYDDFTVRLW
                     DLSTGEEIQVLSGHYGAVTGVAFSPDGLSLVSGSEDGSLRLWRYDPVQALFLPQKIFT
                     GSTQAVTGVAFSPRGDRVAGGCADGLVRIWEVQP"
     gene            1280..>2049
                     /locus_tag="VN363_09445"
     CDS             1280..>2049
                     /locus_tag="VN363_09445"
                     /inference="COORDINATES: protein motif:HMM:NF021507.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="phosphodiester glycosidase family protein"
                     /protein_id="HWQ84780.1"
                     /translation="MRQNPYRTLILASGFIGLILITGLFLAWYWLGGRPQPSEQSQEL
                     YPGVYYSRQVRQSPRPMVIHVVQVDLREEGISLLVTPGDPKAGLPLAARTTSEFLEEF
                     NLQLAVNGDGFLPWRSNAPLDIYPRSGDGVDPIGLAASEGVQYSSFTDNEPVLYLGRN
                     NRAQFNSPGGKVYNAISGNLMLVARGKALPGLEGEPEPRTALALDRRGRTLLIVVVDG
                     RQPGYSEGATLNELAELVVEFGGYQGMNMDGGGSSTLVR"
BASE COUNT          374 a          590 c          652 g          433 t
ORIGIN      
        1 attcagccac gctccagcca accccccacc tattgcctga cctgaccctt taccgggtag
       61 aaattaccct ggaagcggat gatccctgcg ccgatcctga tgccgggctg ggggtgcggg
      121 tatggttgcg aaattcgggc ctggctgact ccgcgccttt cagcatccag gttaatcagc
      181 aggatcaggc catagaatct gccctatcgc cgggtgagca ggcaagcctg tggttcgctg
      241 gctatgagag cctgaatgaa atccgcctgg atgtgtattc gcaagtgatg gaaagcgatg
      301 agaccaacaa tcatgaaatc cgccagctgc tcctgccaac actgcccgct ggctgccagc
      361 cgcaagctca gccggaactg gaatcccagg atccactcta cagcctggag gggcatactg
      421 gcaaggtatg gagcgtggct ttttcccccg atggaggtta cctcgccagt gtgggaatcg
      481 ataattcatt gcgcctgtgg cgggtagatc agggcttctt gctgcgcacc atgcaggggc
      541 atccctaccc gatccgcagc ctggctttca accaccgtgg gacggaaatt gctaccgggt
      601 cgtacgacgg agtggtgcgc atctggcagg tttcgaatgg gatgctgcag cgcagcctca
      661 ccgggcatgc cggcagggtg ctggctctgg cgtattcgcc gaacgatcgc agcctggcga
      721 gcacggctga agattttacc gtgcgcctct ggcgggtgca aagcggtgtg ctgctcaacc
      781 tgattgatga gggcatggag cagatagaaa gtgtggaatt tctgccggat ggctcggccc
      841 tggtttgggg agaagccagc ggtgacctga ggatgtggaa tatctccgat aaccgctggc
      901 agctgcacct gaaaggtctc ggtctgccag tccgcagcat cggattgact gccgatggag
      961 ccctcctggc agccggctat gatgatttta cagtccggct ctgggatttg agcacaggcg
     1021 aggaaataca ggttctgtcc gggcattatg gggctgtcac cggtgtggcg ttctcgcccg
     1081 atgggctcag cctggtcagc ggctcggagg atggctcgct gcgcctctgg cgctatgacc
     1141 cggtccaggc gctctttctg ccccagaaga tatttaccgg ttctacacaa gccgtgaccg
     1201 gtgtagcctt ttctcccagg ggagaccggg tggcaggtgg ctgcgcggac gggttggttc
     1261 gcatctggga agtccagcct tgaggcagaa tccttaccgg actctcattc tggccagcgg
     1321 gttcatcggc ttgatcctga tcacgggtct gtttctggcc tggtactggc ttggcgggcg
     1381 tccgcagcct tccgaacaaa gccaggagct ttacccgggt gtatactatt ctcgccaggt
     1441 ccgccaatcg ccgcgcccga tggtgattca tgtcgtccag gtcgatctgc gggaagaggg
     1501 catctccctg ctggtcaccc cgggcgaccc caaagccggg ctgcccctgg ctgcccgcac
     1561 cacctctgag tttctggaag aatttaacct ccagctggcg gtcaatgggg atggctttct
     1621 gccctggcgc tccaatgctc ccctggatat ctacccccgc agtggggatg gggtggaccc
     1681 gattggtctg gcagcctcgg aaggggtgca gtattcatcg tttaccgaca atgagccggt
     1741 gctgtacctg ggacgcaaca accgggctca gttcaattcc cccggcggaa aagtttacaa
     1801 tgccatctct ggaaacctga tgctggtcgc gcggggtaaa gccttgcccg gattggaggg
     1861 cgagccagag ccgcgcacag ccctggccct ggatcggcgc gggcgcaccc tgttgattgt
     1921 ggtggtcgat ggacgccagc caggctactc cgaaggagcc accctgaacg agctggcaga
     1981 gctggtcgtc gaatttggcg gctaccaggg gatgaatatg gatggcggcg gttccagcac
     2041 cctggtgcg
//
LOCUS       DATMXK010000520         3757 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_94826, whole genome shotgun sequence.
ACCESSION   DATMXK010000520 DATMXK010000000
VERSION     DATMXK010000520.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3757)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3757)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3757
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94826"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..395)
                     /locus_tag="VN363_09450"
     CDS             complement(<1..395)
                     /locus_tag="VN363_09450"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016291381.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003924 - GTPase activity [Evidence IEA];
                     GO_function: GO:0005525 - GTP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GTP-binding protein"
                     /protein_id="HWQ84781.1"
                     /translation="MEQLDHIRNFCIIAHIDHGKSTLADRLLQLTGTISDRDMTEQVL
                     DTMDLEREKGVTIKASAVRMLYTADDGQNYELNLIDTPGHVDFGYEVSRALEACEGAL
                     LIVDATQGIEAQTLANLYFALESDLEIIP"
     gene            complement(479..1321)
                     /locus_tag="VN363_09455"
     CDS             complement(479..1321)
                     /locus_tag="VN363_09455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559998.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0032259 - methylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="TlyA family RNA methyltransferase"
                     /protein_id="HWQ84782.1"
                     /translation="MVKARLDILLVEQGLVESRSLAQRMIMAGQVRVNDQMVLKPAAL
                     VAQDAELSVNQGPPFVSRGGEKLAAALETFPISVAEKICADVGASTGGFTDCLLQHGA
                     VKVYAIDVGHGILHWKLRQDPRVVVMERTNARHLVELPEQVDVVTIDASFISLKVLLP
                     VITGWLKTNGSEIIALIKPQFEAGKSAVARGEGVIRDPNLHRQILQEILTYCEQQGFG
                     VNGLIRSPLLGPKGNVEFLAWFGYPQITQADLSALINAAAPAGAAEPTEPAPGAANVE
                     QGFD"
     gene            1453..2340
                     /locus_tag="VN363_09460"
     CDS             1453..2340
                     /locus_tag="VN363_09460"
                     /EC_number="2.4.2.45"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559999.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016765 - transferase activity,
                     transferring alkyl or aryl (other than methyl) groups
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="decaprenyl-phosphate phosphoribosyltransferase"
                     /protein_id="HWQ84783.1"
                     /translation="MCYHTGMLRQLLKTMRPRQWIKNLVIFAALIFDGQLLNIPAGLR
                     TLAGFAIFCLVSGVVYIINDLADIEADRMHPEKSRRPLASGALSPKIALITAALILII
                     VAPLALLLSDRFALIALVYFGINLAYSKWLKHIPIVDVLTIAMGFVLRVAAGVVLIQV
                     ARFSPWLYVVTTLGALYIGFGKRRAELALLADEANSHRRVLDGYTIPLLDQFIIILSA
                     TTIIAYSLYTFSAPNLPTNHAMMLTIPFVLYGIFRYLYLIQVTHQGGAPEEVLLSDRP
                     LQFVIALWGLTVLIIFYIL"
     gene            2367..3308
                     /gene="mvk"
                     /locus_tag="VN363_09465"
     CDS             2367..3308
                     /gene="mvk"
                     /locus_tag="VN363_09465"
                     /EC_number="2.7.1.36"
                     /inference="COORDINATES: protein motif:HMM:TIGR00549.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004496 - mevalonate kinase activity
                     [Evidence IEA];
                     GO_process: GO:0016126 - sterol biosynthetic process
                     [Evidence IEA];
                     GO_process: GO:0019287 - isopentenyl diphosphate
                     biosynthetic process, mevalonate pathway [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="mevalonate kinase"
                     /protein_id="HWQ84784.1"
                     /translation="MPAFTATAPGKIILFGEHAVVYHRPAVAVPVNQVQARAVVLPDP
                     RAPRGRVWIQAPDIQVDSPLDELPESAPLARLVHIFLEHFSIDHLPACTLRVSSTIPI
                     AAGMGSGAAISVACLRALAAFIGSSLPPEQVSALAFEIEKMYHGTPSGIDNTVVTFGQ
                     PVFFVRDQPIEILSLDKPFMLVIGDTGVRSPTSGPVEAVRRAWKADPGRYEALFDQAG
                     EIARSARNLLESGDPARLGSLMNANQQLLVEMGVSSPELDHLVQAAQDAGALGAKLSG
                     AGWGGNMIALVDPGRAEQVSAALHQHGAVNTLITRVG"
     gene            complement(3392..>3757)
                     /locus_tag="VN363_09470"
     CDS             complement(3392..>3757)
                     /locus_tag="VN363_09470"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84785.1"
                     /translation="LESRSENEPALWTQPEEVDEGLIQGTYPAYTVKAGDHFRATIGC
                     LAGYDRCQVMFQLKYQIGDGEIQTLGSWDESYDGDTTSIDIDLSTLVDQKVKFILIVK
                     ADGTAREDAAFWLVPQISH"
BASE COUNT          781 a         1048 c          957 g          971 t
ORIGIN      
        1 attgggatga tctcaaggtc agattcaagc gcaaaataca aatttgccag tgtctgggct
       61 tcaatcccct gggttgcatc cacaatcagt aatgctcctt cacaggcttc cagcgcccgg
      121 ctgacctcgt aaccaaaatc cacgtgtccg ggagtgtcga tcaaattaag ctcataattc
      181 tgaccatcat cggcagtata tagcatccgg acagccgagg ctttgattgt gacccccttt
      241 tcacgttcca gatccatggt atccagcacc tgttcggtca tatcgcgatc cgatatggtg
      301 ccggtcagct gcaagagccg gtctgccagg gtcgatttgc cgtggtcgat atgcgcaatg
      361 atacagaaat tccgaatatg atctaactgt tccatcaggt gggatgcttt cctgagtatc
      421 taattatgaa tttaaatttg ctcctgcata gttcccgcag aagcattgta ttgtactact
      481 aatcaaatcc ctgctcaaca tttgcagcac cgggcgccgg ctcagttggc tcagcagccc
      541 cggctggggc tgcagcatta atcagcgcgg acagatcagc ctgagtaatt tgtggatagc
      601 cgaaccaggc taaaaattcg acattcccct tgggtcccag cagcggtgaa cggatcagac
      661 cgttgacacc aaatccctgc tgttcgcaat atgtcaggat ttcctgcaga atctggcgat
      721 gcagatttgg atcgcgaatc accccttcac cgcgggcaac agcagatttc ccggcttcga
      781 actggggctt gatcagcgca ataatctcac ttccgttggt tttcagccag ccggtaatca
      841 ctggcagcag tactttgagg gagataaatg aggcatcgat ggtaaccaca tcgacctgct
      901 ctggtaattc gacgaggtgg cgggcgtttg tgcgctccat aaccacaacc cgtgggtcct
      961 ggcgcagttt ccagtgcaag ataccatggc caacatcgat ggcatacact ttaacggccc
     1021 cgtgctgcag cagacaatcg gtgaatccgc cggtcgaggc gccaacatcc gcgcaaattt
     1081 tctcagctac agatattgga aatgtttcca gcgcagccgc taatttctca ccaccgcgcg
     1141 acacaaatgg cgggccctga ttgacgctta gttcggcatc ctgcgccacc agcgcagctg
     1201 gtttcaggac catttgatcg ttcactcgca cctggccggc cataatcatg cgttgggcga
     1261 gtgatcggct ctcgaccagg ccttgttcca ccagcaggat atccagacgt gcttttacca
     1321 tgcgattatt ttatcatccg ccgctcatta catggtgata ggtcaataag ccgtgatatt
     1381 ttttcgatat ggtctcctgc tatagttcct gaccgaagtg ataacctccc cttctatacg
     1441 tacgcgggga gtgtgtgcta tcatacgggt atgctgcgcc agctcttgaa aacaatgcgc
     1501 ccccgccagt ggatcaaaaa tctggtcatc tttgccgcgt tgatcttcga tgggcagctg
     1561 ttaaatattc ccgccggttt gcggaccctg gcaggttttg caattttttg ccttgtatcc
     1621 ggggtcgtgt atatcatcaa tgatctcgcg gacatcgaag ccgaccgcat gcatcctgag
     1681 aaaagccgcc ggcctctggc ttctggtgcg ctctccccaa aaatcgcctt aatcaccgca
     1741 gcccttattc taatcatcgt tgcccccctg gcgctgctgc tctcggaccg gtttgcgctg
     1801 atagctctcg tatattttgg aatcaacctg gcttattcga agtggctaaa acatatcccg
     1861 attgtggatg tattgacaat cgccatgggt tttgttttac gagttgcagc tggagtggta
     1921 ttgatccagg tggcgcgctt ctctccctgg ttgtatgtag tcaccaccct cggtgcattg
     1981 tatataggtt tcggcaagcg gcgggctgag ctggcattac tggcggatga ggcgaattct
     2041 catcgccggg ttttggatgg ctataccatc ccgcttctgg accaatttat catcattctt
     2101 tcagcgacaa cgatcattgc ctacagtctg tatacattct cagcgcccaa tcttcccacg
     2161 aaccatgcga tgatgttgac gatccccttt gtcctgtatg ggatctttcg ctacctgtat
     2221 ttgatccagg ttactcacca gggcggcgct cctgaagaag tgctgctctc agatcgccca
     2281 ctccagttcg tgattgcact ctgggggctg actgtcctga tcatttttta tatcctttaa
     2341 gtcccacatg gaaataatta cccccaatgc ctgcatttac agccacagct cccggtaaaa
     2401 ttatcctttt tggcgagcat gcggttgtct atcatcgccc tgccgttgct gtaccggtca
     2461 accaggtcca ggctcgggct gtcgttctcc cggatccccg ggcgccgcgc ggccgggtgt
     2521 ggattcaagc gcctgatatc caggttgatt ccccgctgga tgaactgcca gagagcgcac
     2581 ccctggcacg cctggtccac atttttctgg aacatttttc gatcgatcat ctgccggcgt
     2641 gcaccttgcg ggtctcttcc acgatcccta tcgcggccgg gatgggatct ggcgcagcga
     2701 tctctgtggc ttgcttgcgg gccctggctg cttttatcgg atcttcgctg cctcccgagc
     2761 aggtatccgc gctggctttc gagatcgaaa agatgtacca tggcactcct tccggcatcg
     2821 ataacacggt ggtgactttt ggtcagccgg tctttttcgt gcgtgatcaa ccaatcgaaa
     2881 ttctatccct ggataagcct ttcatgctgg tgatcgggga taccggcgtg cgcagcccta
     2941 cctcgggtcc ggtggaggct gtccgccgcg cctggaaggc agatccaggc cggtacgagg
     3001 cattattcga ccaggccggc gaaatcgccc gctctgcccg aaatttgctg gaatcaggcg
     3061 atcctgccag gcttggcagt ttgatgaacg ccaatcagca gctcctggtt gagatgggag
     3121 tttccagccc agagctggat catctggtcc aggctgctca ggatgccggg gcattgggcg
     3181 ccaaactgtc cggcgctggt tggggtggaa atatgatcgc cctggttgat cccgggcggg
     3241 cggaacaggt ttccgcagct ctacaccagc atggtgcagt aaacaccctg ataacccggg
     3301 taggataatt cccacatatt gcatacaaaa accgggcatt ctggatggaa cgcccggttg
     3361 tgcaatctcg atctggtatg ttatcgagca gttaatgtga aatttgcggg acaagccaga
     3421 aagcggcatc ctcccgcgcc gtgccatcgg ctttgacgat cagaatgaat tttacctttt
     3481 gatcaaccag cgtggacaag tcaatatcaa tactggtggt atctccatca tagctttcat
     3541 cccagctccc cagcgtttgg atttctccat caccgatctg gtatttgagc tggaacatca
     3601 cctgacagcg gtcgtagcct gccaggcagc cgatggttgc ccggaaatgg tccccggctt
     3661 tgaccgtata cgccggataa gttccctgga tcaagccttc atcgacttct tctggttgcg
     3721 tccataaggc aggctcattt tcagagcgac tttccag
//
LOCUS       DATMXK010000521         3505 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_28336, whole genome shotgun sequence.
ACCESSION   DATMXK010000521 DATMXK010000000
VERSION     DATMXK010000521.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3505)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3505)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3505
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28336"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..567
                     /locus_tag="VN363_09475"
     CDS             <1..567
                     /locus_tag="VN363_09475"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWQ84786.1"
                     /translation="GIVLAAGSSSRYGQVKQLLEWQGESFVHRVSRTALTAGLDPVIV
                     VTGAFADQVTAALTDLPAKCIFNPGWETGQGTSVAAGIQALPPTVGAAVFLLADQPLT
                     PPGLITSLVEAHAIEGNPIIGPLFDGQRGNPVLFDRITFPDLLQLKGDKGGRAIFSRY
                     PVRWIPWQETSGRLDIDTPADYEQMKEQ"
     gene            567..1196
                     /locus_tag="VN363_09480"
     CDS             567..1196
                     /locus_tag="VN363_09480"
                     /inference="COORDINATES: protein motif:HMM:NF024214.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="nucleotidyltransferase family protein"
                     /protein_id="HWQ84787.1"
                     /translation="MPFKTDASEPNLQIGAVVLAAGESRRMGEPKMSLPWGNTTVIAR
                     VCEVLLSSGLTEIWVVTGGGRENVETVLRHLPVKTIFNPQYADNDMVHSLQTGIAVLK
                     PDLLAALVVLGDQPQIEVETVRKIIKAFQETSQPIIVPSYNNRRGHPWLVRQRLWPDI
                     LALQPPATLRNFLNNHTRHIHYVNLNTPSILKDLDTPDDYAREHSASKK"
     gene            1259..1690
                     /locus_tag="VN363_09485"
     CDS             1259..1690
                     /locus_tag="VN363_09485"
                     /inference="COORDINATES: protein motif:HMM:NF012301.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000156 - phosphorelay response regulator
                     activity [Evidence IEA];
                     GO_process: GO:0000160 - phosphorelay signal transduction
                     system [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="response regulator"
                     /protein_id="HWQ84788.1"
                     /translation="MITLTGEGMANQQKTIVIIEDERDTGEMFAEMLRLRGYQAHNAF
                     GGIAALSLIAEKQPDVILLDLMMPDLSGMEILAQLRQDQSLANIPVVIVSAKSMPGDI
                     QAGLKAGAADFLTKPVSFNDLIQAVENVIQAEEDEPDDPSD"
     gene            1700..2293
                     /gene="thpR"
                     /locus_tag="VN363_09490"
     CDS             1700..2293
                     /gene="thpR"
                     /locus_tag="VN363_09490"
                     /inference="COORDINATES: protein motif:HMM:TIGR02258.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008664 - 2'-5'-RNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="RNA 2',3'-cyclic phosphodiesterase"
                     /protein_id="HWQ84789.1"
                     /translation="MAVIRAFIALDLSAEISQSLDQISRELMQRMPQRAIRWVPAKNI
                     HLTLKFLGDVSPSSLESLQKMIQVESENYPPFDISIGGVGAFLSTRRPRVVWVGVTAP
                     ELLSALQHSIESAASRLGYPLEDRPFSPHLTLGRVNRSASPVDLQHVGQVLNGYNIGS
                     LGSVHISGVHLYRSDLSPEGAHYTRLFSAPLKKAPVT"
     gene            2353..2667
                     /gene="rsfS"
                     /locus_tag="VN363_09495"
     CDS             2353..2667
                     /gene="rsfS"
                     /locus_tag="VN363_09495"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559292.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0017148 - negative regulation of
                     translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome silencing factor"
                     /protein_id="HWQ84790.1"
                     /translation="MEVLEDKLAEDILLLDIREISDFTDYFIITSGTSDRMLNALADA
                     VREAVKARFERIGNREGEAREGWIVEDFGDIVVHFFSPDRRDYYRLEELWSKGRVIVR
                     LQ"
     gene            complement(2784..>3505)
                     /locus_tag="VN363_09500"
     CDS             complement(2784..>3505)
                     /locus_tag="VN363_09500"
                     /EC_number="4.2.1.3"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559636.1"
                     /note="Catalyzes the conversion of citrate to isocitrate;
                     Derived by automated computational analysis using gene
                     prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="aconitate hydratase"
                     /protein_id="HWQ84791.1"
                     /translation="IESARVLAVLGDSITTDHISPAGAIPLESPAGMHLIEQGVGVRE
                     FNSYGSRRGNDRVMTRGTFANIRLKNQLAGGTEGGFTRYLGPGGDPEKTVSIYEASLR
                     YKNAGIPLIILAGKEYGSGSSRDWAAKGTLLLGVRAVIAESYERIHRSNLVGMGVLPL
                     QFKAGQSVAALGLTGKEAFSILGLDDQLQHGSEVTVLVERSDGSELLFQVLVRIDTPI
                     EMSYYRNGGILHTVLRNMLQE"
BASE COUNT          867 a          949 c          897 g          792 t
ORIGIN      
        1 ggaatcgtcc tggcagccgg cagcagcagt cgctatggcc aggtgaaaca gcttctcgag
       61 tggcagggag aatcctttgt ccaccgggtt tcccgaactg ctctcaccgc gggactggat
      121 ccagtcatcg tggttaccgg agcctttgct gaccaggtta ctgcggcgct tacagatctg
      181 cctgcgaagt gtatttttaa tcccggttgg gagaccgggc aaggtacttc tgtggccgcc
      241 ggtattcaag cgctgcctcc cacggttggg gcggcggttt tcctgctggc agaccaacca
      301 cttactcccc cggggttgat cacaagcctt gttgaagcgc atgccattga gggaaatccg
      361 atcattggac cgcttttcga tggacagcgg ggtaaccctg ttttattcga ccggatcaca
      421 tttccagatt tactccagct gaaaggagac aaaggtggca gagcgatctt cagccgctac
      481 ccggtacgct ggatcccctg gcaagagacc agtggaagat tagatattga cacacccgcg
      541 gattatgaac agatgaaaga acagtgatgc ctttcaaaac agacgcttcg gagcccaact
      601 tacagatcgg tgctgttgtc ctggcagccg gtgagtcacg ccggatgggc gagccaaaga
      661 tgagtcttcc ctggggaaac acgacggtaa ttgccagggt ttgtgaagta cttctgagca
      721 gcggattaac cgagatctgg gtggtcaccg gcggcgggcg ggaaaatgtc gaaactgtac
      781 tcaggcatct gccagtaaaa acgattttta acccacaata tgccgacaac gacatggtcc
      841 attcgctgca aaccggtatc gcagtactca aaccggatct actggctgcg ctggtcgtgc
      901 tgggcgacca gcctcagatc gaggttgaaa cagtacggaa aatcatcaaa gccttccagg
      961 aaaccagcca gcccatcatc gtacccagct acaataaccg gcgggggcac ccctggctgg
     1021 tgaggcagag attatggcct gatatactgg ctttacagcc tccagccacg ctgcgaaatt
     1081 ttttgaataa tcacaccaga cacatccatt atgtgaatct gaacacgccc agcatcctca
     1141 aagatctgga tacacccgat gattatgccc gcgagcactc tgcctcgaag aagtaatgcc
     1201 tgagtatgac aggcagctcc tgagggtcat gaggtttgac aggaagtaga aatgttgtat
     1261 gattaccctg acgggagagg gaatggcaaa ccagcagaaa acgattgtca taattgaaga
     1321 tgaacgcgat acgggtgaaa tgttcgcaga aatgctgcga ttgcgcggtt atcaggcgca
     1381 caatgccttt ggcgggattg ctgcactgag tttgatcgct gaaaaacagc cagatgttat
     1441 tctattggat ctgatgatgc ctgatctctc cgggatggaa atcctggctc agctgcggca
     1501 ggatcaaagc ctggcgaaca tcccggtcgt gatcgtttcc gcaaaatcaa tgcctggcga
     1561 tatccaggcc gggttaaaag ccggggcagc cgatttcctt accaaaccag tatcctttaa
     1621 cgatcttatc caggccgtag aaaacgtgat ccaagccgaa gaagatgagc cagatgatcc
     1681 ttcggactga ccaacctcaa tggctgtaat ccgcgccttc attgccctgg atctatcggc
     1741 tgaaatttcc caatcactcg atcaaattag ccgcgagctg atgcagcgta tgccccagag
     1801 agctatccgt tgggttcctg ccaaaaacat ccacctgacg ttaaaatttt taggggacgt
     1861 ttcaccatcc agcctggaat cactgcaaaa gatgattcag gtagaaagtg aaaattatcc
     1921 accttttgac atcagtattg gtggagtggg cgcgtttcta tctacccgcc ggccgcgcgt
     1981 ggtctgggta ggcgttaccg ccccggagct gctgtctgcc ctgcagcaca gcattgaatc
     2041 ggccgcctca cggctgggtt atcccctgga ggaccgtcca ttttcgccgc acctcacgct
     2101 gggtcgcgtg aaccgcagcg catctcctgt tgatctccaa catgtcgggc aggttctcaa
     2161 cggttataat ataggctcct tgggctcagt ccatatatct ggagtccacc tataccggag
     2221 cgatctttct ccggaaggcg cgcattatac ccgacttttt tcagcgccgc tcaagaaggc
     2281 gccggtaacc taaaccagta aatattcaaa ccgaggtgaa atctggaaac actggaagtt
     2341 gctcactata tcctggaagt actggaagac aagttagccg aagatatttt attgttggac
     2401 attcgggaaa tctctgactt caccgattat ttcatcatta catccggcac aagtgatcgg
     2461 atgctgaacg cgttggcaga tgcagtccgt gaggcggtaa aagctcgttt cgagcggata
     2521 ggcaaccggg aaggcgaagc ccgagaaggc tggatcgtgg aagattttgg tgacattgtg
     2581 gttcatttct tctcgcccga ccggcgggat tattaccgcc tggaagaatt gtggagtaag
     2641 ggccgcgtaa tcgtccggct gcagtaatat ctttcctatc caaaccatgc aacgaatcaa
     2701 tcaggagctg aaaattctca gctcctgacc actcctataa atttacacag tcttattcaa
     2761 gacggtggta tcgagccagc gcgttattcc tggagcatat tccttaaaac ggtatgtaaa
     2821 atccctccat tgcggtaata gctcatttcg atcggagtgt cgatccggac cagcacctgg
     2881 aataacagct cactgccatc cgaccgctcc accagtacag taacctcgct gccatgctgg
     2941 agttgatcat ctaagccaag aatagagaaa gcttcctttc cggtcaggcc cagagcagca
     3001 accgattgtc ctgccttgaa ctgcaggggt aacacgccca tcccaaccag attagagcgg
     3061 tggatgcgct cataggactc cgcaatcacc gcccttacac cgagcaagag tgtgcctttg
     3121 gcagcccaat ctcggctgga tcccgatcca tattccttgc cagccaggat gatcaaggga
     3181 ataccggcgt ttttatagcg taaggaagcc tcataaatcg agaccgtctt ttccgggtcg
     3241 ccaccaggac ccaggtatcg cgtaaagcct ccctccgtac ccccggccaa ctgattcttc
     3301 aagcggatat tcgcaaaggt accccgcgtc atgacccggt cattcccccg ccgcgaccca
     3361 taagaattaa attcccgcac cccaacaccc tgttcaatca gatgcatacc agccggactt
     3421 tccagcggga tcgcacccgc cggcgaaata tggtcggttg tgatcgagtc acccaaaaca
     3481 gccagcacac gggccgattc aattt
//
LOCUS       DATMXK010000522         3040 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_35395, whole genome shotgun sequence.
ACCESSION   DATMXK010000522 DATMXK010000000
VERSION     DATMXK010000522.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3040)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3040)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3040
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_35395"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..741
                     /locus_tag="VN363_09505"
     CDS             <1..741
                     /locus_tag="VN363_09505"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84792.1"
                     /translation="RNIWIIARREYDQYFASPVAYMIMFVIYLVIGIFFYLNMSFAFV
                     QTQYAPTVQIILAPLATMLVLAVPAITTRLLAEERRLGTIELLLTAPVRDWELVIGKW
                     LGAFLLVVTILGFSLIYPILLNNLVDPGIDQGMLLTGYLGLFLLAAVFTAVGVFVSSL
                     FSNQIAAFATTMGALIFLWWVINPIAQVLGQTSPVGRFFSFIDISDHFFNNFLSGVVD
                     LTQLVYALSLTALALFGASMVLEVRRWR"
     gene            741..2321
                     /locus_tag="VN363_09510"
     CDS             741..2321
                     /locus_tag="VN363_09510"
                     /inference="COORDINATES: protein motif:HMM:NF021351.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Gldg family protein"
                     /protein_id="HWQ84793.1"
                     /translation="MDNKTRIYAPLGLGLAGAAALGAAGVYLVLREFNLTVQILLGLV
                     VVGLALFVILDPDRTRRAFTGRQARYGSNALVLSIAFIGLLVVANLLAAQNPQRWDLT
                     EDKTYTLTDETIAALEQLPAPVKATAFFTQSLDSSSASGLLEQYAYYGKDNFTFSFVD
                     PDADPIAAQTAGISRDGTVVLAMGDPSMGAQTLQVTTISEQELTSGLVRLMNPQASVV
                     YFLTGHGELSPEDTGDRSFSQVKRLLVSKNYAVNTLSLLGTNAIPEDATAIVIAGPTT
                     ALTQAEVDLISAFQANGGGLVVLYEPPVDEASSLGPDDPLAAYLQSQWGVKFDANVVL
                     DPTSQQPQAPYAASYGQHAITAQMNNVSAWFPTARSVTLSEVAGGASQTPLVFSSQQA
                     WGEADIPGLVGWFQTGEGQVQYDEGADVLGPVTLAAAAENTSTQARLVVYGDSDFPSN
                     ANFLAFGNGDLFVNSVDWSAGLEELISLNPRTATTRTVASPQAYTLNLIFLGTVILVP
                     GVALVAGILTFIQRRRRG"
     gene            2326..2934
                     /locus_tag="VN363_09515"
     CDS             2326..2934
                     /locus_tag="VN363_09515"
                     /inference="COORDINATES: protein motif:HMM:NF025598.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4340 domain-containing protein"
                     /protein_id="HWQ84794.1"
                     /translation="MIRRNTLILVGVFLLALAGYVLLNRQQEQAAAQITPTAATQPLF
                     EFEASELTGIQVADSSGREVRLEMMSGAWVLVDPTAEATDTERVTSLATQIASLTARS
                     LIENPPAASAMGLEPPAYTVRLQLGDGSRLTLEVGAETPTQSGYYVRLADGRVAVVST
                     FSVDGITAVLDSPPVYLTPTVGITPFSPDMLPTSGAGTTPTP"
BASE COUNT          550 a          942 c          933 g          615 t
ORIGIN      
        1 cgtaatatct ggattatagc caggcgggaa tatgatcagt acttcgctag ccctgtggca
       61 tacatgatca tgttcgtcat ttatctggta atcggaattt tcttttatct aaacatgagt
      121 tttgccttcg tgcaaaccca atatgccccc acagtgcaga tcatcctggc accgctggcg
      181 accatgctcg tcctggctgt gccggcgatc accacccgcc tgctggcgga ggagcgccgc
      241 ctgggcacga tcgagctgct gctcaccgcc ccggtgcggg attgggagct ggtgatcggc
      301 aagtggctgg gggcgttcct gctggtggtg accatcctgg gtttcagcct gatctacccg
      361 atcctcctta ataacctggt ggacccgggc atcgatcagg ggatgctgct taccggttac
      421 ctggggctct tcctgctggc agccgtgttc acggcggtgg gcgtgtttgt ctcgtccctg
      481 ttcagcaacc agattgccgc ctttgccacc acgatgggcg cgctgatctt cctgtggtgg
      541 gtgatcaacc cgatcgccca ggtgttgggc caaaccagcc ccgtggggcg gttcttttcc
      601 tttatcgata tcagcgacca tttctttaat aacttcctga gcggcgtggt cgacctgacc
      661 cagctggtct atgcgctctc gctcaccgcc ctggcgctgt tcggcgccag catggtcctg
      721 gaagtgcgga ggtggcgctg atggacaaca aaactcgcat atacgccccg ctggggctgg
      781 gattggccgg agccgccgcc ctgggtgcag ccggcgtgta cctcgtgctg cgcgaattca
      841 atctcaccgt gcagattctg ctcgggctgg tcgtggtggg gctggcgctg ttcgtgatcc
      901 tggatcccga ccgcacccgg cgtgcgttca ccgggcggca ggcgcgctac ggctcgaatg
      961 ccctggtgct aagcatcgct ttcatcgggc tgctggtggt cgccaacctg ctggcggcgc
     1021 agaatcccca gcgctgggac ctgaccgagg acaagaccta cacactcacc gacgagacga
     1081 tcgccgcgct ggaacaactg cccgcgccgg tgaaagccac ggcgtttttc acccagagcc
     1141 tggacagcag ctcggccagc gggctgctgg agcagtatgc ttactacggc aaggataatt
     1201 tcaccttcag cttcgtcgac ccggatgccg atccgattgc cgcacagacg gcaggcatct
     1261 cccgggatgg cacagtggtg ctggccatgg gcgacccaag tatgggggca cagaccctgc
     1321 aggtgaccac catctcagag caggagctga cctccggact ggtacggctg atgaacccgc
     1381 aggccagcgt ggtctatttc ctcaccggac acggtgagct ctctcccgag gataccggcg
     1441 accggtcctt cagccaggtc aaacgcctgc tggtgagcaa gaattacgcc gtgaatacgc
     1501 tcagcctgct ggggacgaat gccatccccg aagacgccac tgcgatcgtg attgccgggc
     1561 caacgactgc cctgactcag gcggaagtgg acctgatcag cgcgttccag gcgaacggcg
     1621 gcgggctggt ggtgctctac gagcctcctg tcgatgaagc ttcttccctg ggtccggacg
     1681 acccgctggc agcctacctg cagagccagt ggggagttaa attcgatgcc aacgtggtcc
     1741 tggatcccac ttcgcagcag ccgcaggcgc cttacgccgc cagctacggg cagcatgcga
     1801 tcaccgccca gatgaacaat gtgtccgcct ggttcccaac ggcccgcagc gtgacgctga
     1861 gtgaagtggc cggcggggca tcccagaccc cgctcgtatt cagctcccag caagcctggg
     1921 gcgaagcaga tatccccggc ttagtcggct ggttccagac cggcgaaggc caggtgcagt
     1981 acgatgaagg cgcggatgtc ctgggtccgg tgaccctggc agcggcagcc gagaatacga
     2041 gcacccaggc ccgcctggtg gtatacggcg acagcgattt tccgtcgaat gccaatttcc
     2101 tggctttcgg caacggcgac ctgttcgtga actcggtgga ctggtcggcc gggctggaag
     2161 agctgatcag cctgaacccg cgcacggcga cgacccgcac cgtggcgtcc ccgcaggcct
     2221 acacgctgaa cctgatcttc ctgggcacgg tcatcctggt gccaggcgtg gccctggtgg
     2281 ccggcatttt gaccttcatc cagcgccgcc ggcgaggcta agcccatgat ccggcggaac
     2341 acactgattt tagtcggcgt attcttgctg gccctggcag gctatgtgct gctcaaccgc
     2401 cagcaggagc aggccgcagc gcagatcaca cccaccgcag ccactcagcc gctgtttgag
     2461 ttcgaagcca gcgagctgac cggaatccag gtggcagact cctccgggcg tgaagtgcgc
     2521 ctggaaatga tgtcgggagc ctgggtgctg gtcgacccaa ctgcagaagc gaccgatacg
     2581 gagcgggtga cctccctggc cacccagatt gccagcctga ccgcccgcag cctgatcgag
     2641 aatccgccgg cggcgagcgc catgggcctg gaacccccgg cgtacaccgt tcgtctacag
     2701 cttggggatg gcagccgcct gacgctggaa gtaggggccg agacgcccac ccaatccggt
     2761 tattatgtcc ggctggcgga tggccgagtg gcagttgtgt ctaccttcag cgtggatggg
     2821 atcactgcag tactggacag tcctccggtg tacctgacgc cgacggttgg catcaccccg
     2881 ttttcacccg acatgctgcc aacatctggg gctggtacaa cacccacccc gtaaatctgg
     2941 cagttttgtg tgttagaaat ggataaatgt tgactttgtg tattgcattt cgattaaaaa
     3001 aagtgtattc tcattctatg atcgtgtgta ctaaatagcg
//
LOCUS       DATMXK010000523         4297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_142367, whole genome shotgun sequence.
ACCESSION   DATMXK010000523 DATMXK010000000
VERSION     DATMXK010000523.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142367"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..3219
                     /locus_tag="VN363_09520"
     CDS             <1..3219
                     /locus_tag="VN363_09520"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559423.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0032549 - ribonucleoside binding [Evidence
                     IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit beta"
                     /protein_id="HWQ84795.1"
                     /translation="VDNNPDRMFIASTLYQEPEWDLSHGHTIAEAALIEFFKRMRPGD
                     PATLDNARGFLKEQLFDQRHYDLERVGRYKLNQKLELQEHVPIIHRMVTKWDIVRLVR
                     RMIMINNGSVMADDIDHLGNRRAKTVGELIQNKLRIGLRRMERVIKERMSIRDQDQLS
                     PVTLINIRPVVAALREFFGSSQLSQFMDQTNPLAELRHKRTLSALGPGGLRRERAGFD
                     VRDVHYSHYGRICPIETPEGPNIGLIGRLASFARVNEYGFIETPYRRVRKALQPDDPR
                     LIDRMLRENIVEPKTGEVIAQEGERITEELAKKIRKINSPEVLIVPFVTAEMEYLSAD
                     VEDRFVIAQANTPLNEYKEFIRTRVSSRYYSSFIFSANTSLDYMDVAPHQIVGISAAL
                     IPFLEHDDANRALMGSNMQAQAVPLLTPDVPQVSTGMEYHAAMDSGQVIIAEEDGEVT
                     SVTGSKITIRNNESKEHSYLLRKYQRSNQSTCIDQRPAVVKGQRVKRGDVIGDSSSTS
                     NGQLALGQNVLVGFLSWEGGNFEDAILISERLVQDDRFTSVHIEKYEVESRDTRLGPE
                     EITRDIPNVGEDAIKDLDDRGIIRIGAEVTPNDILVGKISPKGEKELTPEERLLRAIF
                     GEKSRDVKDTSLRMPHGERGKVVDVKVFTREDNADLSAGVDMMVRVSVAQRRKITAGD
                     KMAGRHGNKGVVSRVVPVEDMPFLEDGTPIDIILNPLGVPGRMNIGQVLETHLGWAAR
                     ELGFRAVSPVFDGATEEEIEAELGRAWMIDQAWAETAEKAWAWLAEQGYDPEAIQDDD
                     EVRRMYLEGWLGDRGYDIYQLISDPTYTRHAVLKEWLRDQGYEPDTIFSSELFSKVGA
                     ERDARAEQAINSCLELWSIRKGMEIKGLSPEEIFKRASQITLDTGEPIPTLGKQLLRD
                     GKTGQAYDQPVTVGVMTMLKLHHLVEDKVHARSTGPYSLVSQQPLGGKAQFGGQRFGE
                     MEVWALEAYGAAYTLQEMLTVKSDDVQGRVKTYEAIVKGEPIEEPSIPASFRVLVKEL
                     QSLGLAVEAVTDTGELIRFGKDEERARPPRPSTGLLGLGEEL"
     gene            3417..3833
                     /gene="rpsL"
                     /locus_tag="VN363_09525"
     CDS             3417..3833
                     /gene="rpsL"
                     /locus_tag="VN363_09525"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_007682177.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S12"
                     /protein_id="HWQ84796.1"
                     /translation="MPTINQLVRKGRKSKNVKSKAPALLYTLNSMNQHRNRQDKGAPQ
                     KRGVCTNVRTMTPKKPNSALRKIARVRLSNGIEVTAYIPGEGHTLQEHSVVLVRGGRV
                     KDLPGVRYHIVRGTLDTGGVNNRRQSRSKYGSKQPK"
     gene            3908..>4297
                     /gene="rpsG"
                     /locus_tag="VN363_09530"
     CDS             3908..>4297
                     /gene="rpsG"
                     /locus_tag="VN363_09530"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559425.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000314 - organellar small ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022627 - cytosolic small ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S7"
                     /protein_id="HWQ84797.1"
                     /translation="MSRRNKPEKRQVLPDPRYNSVQLQSFINRVMRNGKRSTATSLVY
                     GAFDMIEGKGNRAALDIFDQALKNVSPMVEVKPRRVGGATYQIPMEVPPYRRFALATR
                     WILQAARSRSGKNFSEKLAGELMDAANN"
BASE COUNT         1058 a         1062 c         1202 g          975 t
ORIGIN      
        1 gttgataata accctgaccg gatgttcatt gcttctactc tgtaccagga accagaatgg
       61 gatctttccc atgggcacac cattgccgaa gcagccctga tcgagttttt caagcggatg
      121 cgtccgggtg atccggcgac cctggacaac gcccgcggtt tcctcaagga gcagttattc
      181 gatcagagac attatgacct ggagcgagtt gggcggtata agcttaacca aaagctcgaa
      241 ctgcaggagc atgttccgat tattcaccgc atggtgacaa aatgggatat cgtacgcctt
      301 gtacggcgaa tgatcatgat caataatggc tccgtcatgg ccgatgatat cgaccatctc
      361 gggaatcgcc gggctaagac cgtcggtgaa ctgatccaga acaagctgcg gattggtctg
      421 cgccgcatgg agcgggtgat taaagaacgt atgtcaatcc gcgaccagga ccagctctct
      481 ccagtaacgt tgatcaacat tcgtccagta gtagcagcct tgcgcgagtt cttcggctcc
      541 agccagctat ctcaatttat ggaccagact aaccccttag ccgagctgcg gcataagcgg
      601 actctgtcag cgctgggtcc gggtggcttg cgccgtgaac gagctggctt tgatgtgcgc
      661 gatgtgcact actctcacta tggccggatc tgcccgattg aaaccccgga aggaccgaac
      721 attggtttga tcggtcgcct ggcttcattt gcccgggtaa atgaatatgg gtttattgag
      781 acaccctacc ggcgagttcg gaaagcatta caacctgatg atccgcgctt gatcgaccgg
      841 atgctgcgcg aaaacattgt cgaacctaaa acaggtgaag ttattgctca agaaggcgag
      901 cggatcactg aggagctcgc gaagaaaatt cgcaagatca attctcccga agtgctgatc
      961 gttccttttg taacggctga aatggaatat ctttcggcgg acgtagaaga tcgctttgtc
     1021 atcgctcagg cgaatacacc gctgaatgaa tacaaagaat ttatccgtac acgtgtgtct
     1081 tcgcgatact attcctcatt cattttctca gcgaatacca gcctcgatta catggacgtc
     1141 gctccacacc agattgtggg tattagcgcg gccttaatcc cgttccttga acacgacgat
     1201 gccaaccggg cgttgatggg ctccaatatg caggctcagg cagtacccct gttgactccg
     1261 gatgtgccgc aggtttcgac gggtatggaa tatcatgccg caatggactc gggtcaggtt
     1321 attatcgcag aagaagatgg tgaagtgact tccgttaccg ggagcaagat tactatacgg
     1381 aacaacgaaa gcaaagaaca ttcttacctg ctgcgtaaat accaacgctc caaccagagc
     1441 acctgcattg accaacgacc cgcagttgtt aaggggcagc gcgtcaagcg aggtgatgta
     1501 attggcgatt catcctcaac gagtaatggt cagctggcgc tggggcagaa cgtactggtg
     1561 ggtttccttt cctgggaagg cggcaacttc gaagacgcca ttctgatctc tgagcgcctg
     1621 gtccaggacg atcgttttac ctcagtccat attgagaaat atgaggttga gtcgcgcgat
     1681 actcgcctgg gaccggaaga aatcacccgg gatattccga atgttggtga agatgcgatc
     1741 aaagatctcg atgaccgtgg catcattcgg attggtgcag aagtaacccc caatgatatc
     1801 ctggtaggaa agatctcccc gaagggcgaa aaggaattaa ctccggaaga acgccttttg
     1861 cgggcaattt ttggcgagaa atcacgcgat gtgaaagata cctccttgcg catgccgcac
     1921 ggtgagcgtg gcaaggttgt tgatgtgaag gtattcactc gcgaagacaa tgctgatctc
     1981 tcagctggtg tcgacatgat ggtccgggtt tcggttgccc aacggcgtaa gataactgcc
     2041 ggggataaga tggccggacg ccatggaaat aaaggtgttg tttcccgggt ggtcccggtc
     2101 gaggatatgc cgttccttga ggatggcacg ccgatcgata ttatcctgaa cccactgggt
     2161 gtaccaggtc gtatgaatat cggtcaggtc ctggagaccc acttgggctg ggctgcgcgc
     2221 gagcttggtt tccgagcagt gtcgccggtg tttgatggcg caaccgaaga agaaatcgaa
     2281 gctgagctgg ggcgagcctg gatgatcgat caggcatggg ctgaaactgc cgaaaaggcc
     2341 tgggcatggc tggcggagca gggctacgat ccagaggcca tccaggacga cgatgaagtt
     2401 cgccgaatgt accttgaagg ctggttgggc gaccgcggct atgatatcta ccagttaatt
     2461 tctgacccca cctatacccg ccatgccgtt ttgaaagaat ggctgcgcga tcaaggttat
     2521 gaaccggata caatttttag ctctgaatta ttcagcaagg ttggggctga gcgggatgcg
     2581 cgggctgaac aggcgattaa ttcctgtctc gagctctggt caatccgtaa aggtatggag
     2641 attaaaggct taagccccga agagattttc aagcgtgcct ctcaaatcac cctggatacg
     2701 ggcgagccaa tccctaccct gggtaagcaa ctcctgcgcg atggcaagac cggccaggca
     2761 tatgaccagc ccgtcactgt aggtgtaatg acgatgctca agctgcacca cctggttgag
     2821 gataaagttc acgctcgttc gactggcccg tacagcctgg tttcccaaca acccctgggt
     2881 ggtaaagccc aatttggtgg gcagcgtttc ggtgagatgg aagtttgggc attggaagcc
     2941 tatggtgcag catacactct ccaggaaatg ttgaccgtca aatcggacga tgtccaggga
     3001 cgggtgaaga catatgaagc catcgtaaag ggagagccaa tcgaagaacc aagcatcccg
     3061 gcgtctttcc gggtactggt gaaggaattg caaagccttg gtctggctgt ggaagcagtg
     3121 acggatactg gcgagctgat ccggtttggt aaagatgaag agcgggcccg tccacctcgg
     3181 ccatcaacag ggcttttggg gctgggcgaa gaactataga ccgaatctcc tgcgcccagg
     3241 tccttgactc taaaaattgc gcatggtaga atcgcttttc gttacactaa aataggcggc
     3301 aggacccccg ccatcttcaa tcaggttaac attaccggtg aggccatcag gctagttgga
     3361 tggcctcact taatggaaaa aattagagcg taacagtact tatttcggag gcgaaggtgc
     3421 caacaatcaa tcaattggtc cgtaaaggcc gcaaatcgaa gaatgtcaag agcaaagctc
     3481 ctgcgttgct gtatacatta aattcaatga accagcatcg caatcggcag gacaagggtg
     3541 cgccgcaaaa acgcggggta tgcaccaatg ttcgcaccat gacgcctaaa aaaccgaact
     3601 cggcgctgcg gaagatcgcc cgtgtgcgcc tttcgaatgg catcgaggtg acggcatata
     3661 tccctggcga aggtcatact ttgcaggagc actcagtcgt gctggtacgt ggtggtcgtg
     3721 tgaaagatct ccccggtgtc cgctaccata ttgtgcgcgg aacactggat actggtggtg
     3781 tcaataaccg ccgccagtct cgttcaaagt acggctcgaa gcagcctaaa tagtcagatt
     3841 ggctttctgg gatcgtcagc agtaaccttt tggctggcga ttctcagtgc taaagaggag
     3901 tggttaaatg tctcgacgga ataaaccgga aaaacgccag gttcttcccg acccacgcta
     3961 caacagcgtg cagctgcagt ccttcatcaa ccgggtgatg cgcaatggca agcgcagcac
     4021 agcaacttcc ctggtttatg gtgcattcga tatgatcgag ggcaagggaa atcgcgcggc
     4081 gctggatatt tttgatcagg cactcaagaa tgtatccccc atggttgaag tcaagcctcg
     4141 ccgggtgggt ggtgcaactt accagatccc aatggaagtt cccccttacc gtcgattcgc
     4201 tctggcaacg cgctggatac ttcaagcagc ccgttcccgc tcgggaaaga acttttctga
     4261 gaaactggct ggtgagttaa tggatgctgc caataat
//
LOCUS       DATMXK010000524         2577 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51772, whole genome shotgun sequence.
ACCESSION   DATMXK010000524 DATMXK010000000
VERSION     DATMXK010000524.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2577)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2577)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2577
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51772"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            124..1359
                     /locus_tag="VN363_09535"
     CDS             124..1359
                     /locus_tag="VN363_09535"
                     /inference="COORDINATES: protein motif:HMM:NF012766.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M24 family metallopeptidase"
                     /protein_id="HWQ84798.1"
                     /translation="MKSDLNHLMEINNLDALWITGNGQHNPAMVYLTGGGHFNNADLF
                     IRRNADPVLFCNPMEREEAAKSGFTVRNIGEYRPAELLKQAGGDLLQATIARYKLMFA
                     DLGLEQAQIGVYGQLDAGYAHAVMSGIHEALPGLVFTSDLQGKVLLAAMATKDTTEVE
                     RIRRMGQITTQVVGETADFLSRHSVHDEVLIKKDGTPLTIGEVKGRINLLLAERGAEN
                     PEGTIFAIGRDAGIPHSTGAPGDLLRLGQTIVFDIFPCEAGGGYYYDFTRTWCLGYAP
                     DPAQALYENVLSVYQQIMKELRLGAACSEYQLRTCEMFEAQGHVTIRTDPQTQSGYVH
                     SVGHGLGLHIHERPFMRLNGAEDDLLVPGVIVTVEPGLYYPERGLGVRLEDTVWVRPD
                     GKMEIPAPYPLDLVIPVGK"
     gene            1389..2486
                     /gene="tsaD"
                     /locus_tag="VN363_09540"
     CDS             1389..2486
                     /gene="tsaD"
                     /locus_tag="VN363_09540"
                     /EC_number="2.3.1.234"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559623.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA
                     (adenosine(37)-N6)-threonylcarbamoyltransferase complex
                     transferase subunit TsaD"
                     /protein_id="HWQ84799.1"
                     /translation="MKKSTVPADSNASSASSTPVVRILAIESSCDETAAAVVENGRSI
                     ISSIVASQADLHARYGGVFPEVASRMHIQAIYPVVQQTLQDAFLSLTDVDAIAVTRGP
                     GLPGSLVVGMNVAKGLALSTGLPLIGVNHLEAHLYSAWLYNENQPSTGSMQSPPEPPE
                     FPLLALIVSGGHSELVLMRDHLTYQFLGSTLDDAAGEAFDKVARLIGLDYPGGPAIQK
                     ASEHGNPRAFAFPRAWLDETWNFSFSGLKTAVLREVRKLEQVRSELPVADLAASFQAA
                     VVEVLVSKTLRAAAAFEAKEILVAGGVSANRALRNAFQAQSTYRVHIPPIELCTDNAA
                     MIGGVGYYRFVHGQRDGLEMDVLPNWPLFEL"
BASE COUNT          571 a          717 c          725 g          564 t
ORIGIN      
        1 acccgcgcac agttctcccc gaacaagaat ccgcgctgct taaatcgctc actgagctca
       61 tcacctttca cacccgccgc gccagcgggt aagctgcaaa atttgcaacg acaaggattc
      121 gatatgaaaa gcgacctgaa ccatctgatg gaaattaaca atctggatgc cctgtggatt
      181 accggtaacg ggcagcataa ccctgccatg gtatatctga ccggaggtgg acattttaac
      241 aatgccgatt tatttatccg ccggaatgcc gatccagtgc tcttttgtaa tccaatggaa
      301 cgcgaagaag ctgccaaaag cggctttacg gtccgtaata tcggcgagta tcgtccagct
      361 gagctgttga aacaagcggg cggcgaccta ctgcaggcga ccatcgcacg ttataagctg
      421 atgtttgcag atctgggcct ggagcaagca cagatagggg tgtatgggca attggatgct
      481 ggttatgccc atgctgtgat gtccggcata cacgaagctt taccaggcct ggtctttacc
      541 tccgacctgc agggcaaggt tttgctggcc gcgatggcaa ccaaggacac aaccgaagtg
      601 gagcgcatcc gccggatggg gcaaattaca acccaggttg tcggcgaaac tgccgacttc
      661 ctcagccggc attctgtaca tgacgaagta ttgatcaaga aggatggtac tcccctcacg
      721 attggtgagg tcaaaggtcg gatcaacctg ctgctggcag aacgcggcgc ggagaatcct
      781 gaaggaacaa tctttgcgat cgggcgggat gctggcattc cgcacagcac tggagcgcca
      841 ggtgatttgc tgcgcctggg gcagacgatc gtttttgata ttttcccctg cgaagctggc
      901 ggtggctatt attatgattt cacccgcacc tggtgtctgg gctatgcccc tgaccctgcc
      961 caggcgctgt atgagaatgt cctctcggtt taccagcaaa tcatgaaaga gcttcgcctg
     1021 ggtgcagcct gcagcgagta ccagctgcga acctgtgaaa tgtttgaagc tcaagggcat
     1081 gtgaccatcc ggaccgaccc ccagactcaa tcaggatatg tgcacagcgt cggacatggc
     1141 ctgggactgc acattcatga gcgaccattt atgcgcttga atggcgcaga agatgatttg
     1201 ctggtacctg gtgtgattgt cacggtggaa cctggtctct actatccaga acgagggctg
     1261 ggcgtgcgcc tggaagatac agtctgggtt cgcccagatg gcaagatgga gattccagcg
     1321 ccttatcccc tggatttagt gatcccggtc gggaaataag tcgttttcca gaaagtcggg
     1381 tagatgagat gaaaaagagc acggttcctg ccgattcgaa tgcctcatca gccagcagta
     1441 ccccggtggt gcgcatactg gcgattgaat catcctgcga cgagacggct gcagccgtcg
     1501 tggaaaatgg gcgcagtatc atctccagta ttgtcgcctc ccaggctgat ctgcatgctc
     1561 gctatggagg cgtcttcccc gaagttgcct cccgaatgca tattcaggca atctaccccg
     1621 tggtccaaca gaccttacag gatgcatttt tgagcctgac cgatgtggat gccattgcag
     1681 tcacccgcgg ccccggcttg cctggctcgc tggtcgtcgg catgaatgtt gccaaggggt
     1741 tagccctttc aaccggactg ccgctgatag gcgtcaacca cctcgaagcg catctctatt
     1801 ctgcctggct gtacaacgaa aaccagccca gcaccgggag catgcagagc cctcccgaac
     1861 cacccgaatt ccccctcctg gcgctgatcg tatcgggagg ccactcggag ctggtgctga
     1921 tgcgcgatca cctgacatac cagtttctcg gttccaccct cgacgacgca gccggggagg
     1981 cattcgacaa ggtagcccgc ctgatcgggt tggattatcc gggcgggccg gcgatccaaa
     2041 aggcaagtga acacggtaac ccgcgcgcgt ttgctttccc gcgcgcctgg ctggatgaga
     2101 cgtggaattt ttctttcagt ggattaaaaa cagccgtgct ccgtgaagtc cggaaacttg
     2161 aacaggtgcg cagcgaactg ccggtcgcag atctggcagc cagcttccag gcagcggtgg
     2221 tcgaagtcct ggtcagtaag accctgcggg ctgcggctgc cttcgaagcc aaagaaattc
     2281 tcgttgctgg cggagtatct gcaaaccggg ccctccggaa tgcatttcaa gctcagtcca
     2341 cctaccgggt acacatcccc ccgattgagc tctgcacaga caatgccgca atgattggcg
     2401 gtgtggggta ttacaggttc gtgcacggcc agcgggatgg cctggagatg gatgtgctcc
     2461 ccaactggcc gctgttcgag ctataagggt ttccccagct tttcaattaa atcaactaac
     2521 tggctggcca gctctgaacg atcgaaatgt tcttcaacat agcgccgccc tctggcc
//
LOCUS       DATMXK010000525         2059 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_147366, whole genome shotgun sequence.
ACCESSION   DATMXK010000525 DATMXK010000000
VERSION     DATMXK010000525.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2059)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2059)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2059
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_147366"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1020
                     /locus_tag="VN363_09545"
     CDS             <1..1020
                     /locus_tag="VN363_09545"
                     /inference="COORDINATES: protein motif:HMM:NF037564.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3048 domain-containing protein"
                     /protein_id="HWQ84800.1"
                     /translation="LEAQLIVSSENLPPDTNPLTGLQVADPSVLDRRPVAIKVTNFPR
                     HVRPQWGLSLADHVFEYYLEDLITRFIAVFYGNNAERVGPVRSGRFFDEHIVRMYKSF
                     FVFAWADDRVVDPWTESDLRNFLVVERGDNCPPLCRFGPAYDYNTLFVDTLALEESIA
                     AGRTSNNRQDLSGLSFGSVPPYSPQAGEQVYVRFSDFSYNRWDFDPASRRYLRYEDTL
                     DAYRVPPVYAALTDSLTGLPIAADNLVILLVRHDYFANTPTTEMVTMNLLGEGAAYAL
                     RDGRIYAARWERLAATDLLRLTYPDGSPYLLKPGNVWYEVLGESSTMIQDSVSKTWQF
                     DFAIP"
     gene            complement(1224..>2059)
                     /locus_tag="VN363_09550"
     CDS             complement(1224..>2059)
                     /locus_tag="VN363_09550"
                     /EC_number="1.3.5.2"
                     /inference="COORDINATES: protein motif:HMM:TIGR01036.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004152 - dihydroorotate dehydrogenase
                     activity [Evidence IEA];
                     GO_process: GO:0006222 - UMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="quinone-dependent dihydroorotate dehydrogenase"
                     /protein_id="HWQ84801.1"
                     /translation="GLAALGFGHIEIGTVTPLPQSGNPRPRVFRLPPAQGLINRMGFP
                     GLGAEFVARQLAGKKRPPGLVLGVNLGKNKATPNEEAASDYIRLIQVFAPLADYLAIN
                     ISSPNTVGLRELQGKAALEALLAEIAAERKAQAARLNRPVPVLVKLAPDLSDTELDDA
                     LEAILSAGMDGVIATNTTTSRGPLLRYPGVNPHTAAETGGLSGAPLEAMSSAMVAKIV
                     QRCGGRLPVIGVGGIMDAAGARRKLDAGAVLVQVYSGLVYAGPGLVQQIMRGLKPDPI
                     D"
BASE COUNT          372 a          616 c          600 g          471 t
ORIGIN      
        1 cttgaagcac agctgatcgt cagctcggaa aaccttcccc cggatacaaa cccgctcacc
       61 ggactgcagg tcgccgatcc gtctgtactg gatcgccgcc cggttgccat taaggtcacc
      121 aattttcccc gtcatgtccg cccccagtgg ggcctgagcc tggctgacca cgtctttgag
      181 tattatctgg aagatctgat cacgcgcttc attgcggttt tttatggaaa caacgccgag
      241 agggtcgggc ctgtgcgctc ggggcgcttc tttgatgagc atatcgtgcg catgtataaa
      301 tccttctttg tttttgcctg ggcggatgat cgcgtggtcg atccctggac ggaatccgat
      361 ttgcgcaatt ttctggtcgt cgagcgtgga gataactgcc caccgctgtg ccgctttggt
      421 ccggcttatg attacaacac cctgtttgtg gataccctgg ctctggaaga atccatcgcc
      481 gccggtcgca ccagtaataa ccggcaggat ctgagcgggc tgagctttgg gtccgttcca
      541 ccttattccc cccaggccgg ggagcaggtg tacgtgcgct tttcggattt ttcgtataat
      601 cgctgggatt ttgatcccgc atccaggcgc tacctgcgct atgaggatac cctggatgcc
      661 taccgtgttc caccggtcta tgccgcactg acggacagcc ttacggggct gccgatcgca
      721 gccgataatc tggtcatttt gctggtccgg cacgactatt ttgccaatac gccgaccacc
      781 gaaatggtga ctatgaattt actcggcgaa ggtgccgcat atgcactccg ggatgggcgg
      841 atttacgcag ctcgctggga gcgtctcgcc gcgaccgact tactccgcct gacgtatccg
      901 gatggcagtc cgtacctgtt gaaacctggc aatgtctggt atgaagtatt gggggagagc
      961 tctaccatga tccaggactc ggtttccaaa acctggcagt tcgatttcgc gatcccataa
     1021 accaggcggg tttccacatt gggaaagatt gcctggttgc agaaccggcg gataatgagt
     1081 taaccacaga tggcgcagat aaaatcaaaa gttatctgcg tgcatctgcg aaatctgcgg
     1141 ttgatttttt gtaaggtaaa ccaggtaatc ttcgcgtttg cggcgaaggc ttacgggcat
     1201 cgatcgtgat atgcatacag cttttaatct atcgggtctg gcttcaggcc tctcataatc
     1261 tgctgcacca gacccggccc ggcgtacacc agcccgctgt acacctggac cagcacagcc
     1321 ccggcgtcca gcttgcgccg cgctccggcc gcatccatga ttccccctac cccgatcacc
     1381 ggcagcctgc ccccacagcg ctgtacaatt tttgcgacca ttgcgctgct catcgcttcg
     1441 agcggcgcgc cgctcaaacc accggtttcg gcggctgtgt gcggatttac acccggatac
     1501 ctcagtagcg gcccgcggct ggtggtggta ttggtcgcga tcacgccatc catcccggcg
     1561 ctgagaatgg cttccagggc gtcgtccagc tcggtgtcgc tcaggtcagg cgccagtttt
     1621 accagcaccg ggaccgggcg attgaggcga gctgcttggg ctttgcgttc agcggcgatc
     1681 tctgccagca gggcctccag ggcggctttg ccctgcagct cgcgcagccc gacggtgttg
     1741 ggcgagctga tattgatggc caggtaatct gccagcggcg caaacacctg gatcagcctg
     1801 atatagtcgc tggcagcttc ctcgttgggg gtggctttgt ttttgcccag gtttacgccc
     1861 aggaccagac ccggcgggcg ctttttcccg gcgagctggc gggcgacgaa ctccgcgccc
     1921 aggccgggga agcccatacg gttgatcaat ccctgagccg gtggcaggcg aaacacccgc
     1981 ggccgggggt tgccgctctg gggcaagggc gtcacggtgc caatctcaat gtgcccgaag
     2041 cccagggctg ccaggccgc
//
LOCUS       DATMXK010000526         2665 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_169514, whole genome shotgun sequence.
ACCESSION   DATMXK010000526 DATMXK010000000
VERSION     DATMXK010000526.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2665)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2665)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2665
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_169514"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..495
                     /locus_tag="VN363_09555"
     CDS             <1..495
                     /locus_tag="VN363_09555"
                     /inference="COORDINATES: protein motif:HMM:NF017530.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0070273 - phosphatidylinositol-4-phosphate
                     binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GPP34 family phosphoprotein"
                     /protein_id="HWQ84802.1"
                     /translation="VLKNTEPTGDDLLDEAINDIHQVEKPHRPAYWISQFIAKKKKLR
                     EQLGIRLASQGYLHQEDKRFFWIFKEDEADNPMAPFKYQLKSDLRAKTLTTSANDAHS
                     LALLKILTASGLLGLVFTADEHALATRTINEKVIRAALETPELQTIEEIGQGIAITIE
                     DELD"
     gene            complement(568..1506)
                     /locus_tag="VN363_09560"
     CDS             complement(568..1506)
                     /locus_tag="VN363_09560"
                     /inference="COORDINATES: protein motif:HMM:TIGR01297.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008324 - monoatomic cation transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation diffusion facilitator family transporter"
                     /protein_id="HWQ84803.1"
                     /translation="MSSIVNQEMHEEKNIQRHKKSMLAVNFGLAANIFLAIIKTAVGI
                     LGSSPALLADGINSTSDVVSYLVVAIFLRFSSKPADREHPFGHSQFEAIASLTVGSFV
                     ITTAVGIFWNSVNSIYEQLTGAVVSQGASLIALWVAMLTIALKIGLFIYTNRLSRQLN
                     NTAIMALAYDHRNDIFSASAALIGITLGRMGYSWVDPLAGALVALVILRTGIQILRES
                     ASELMDTVPGEELQKQVEESLAGISGIKSVEDINAHRFGPYLMMNLTLAVDGSLTVSE
                     GDRISTLAERKLKSTVDFLQVVHVHYHPEIRERNQL"
     gene            complement(1568..>2665)
                     /locus_tag="VN363_09565"
     CDS             complement(1568..>2665)
                     /locus_tag="VN363_09565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560669.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ84804.1"
                     /translation="IIGLIEGVAEATASILKLFSGWLSDKLGARKWLAVAGYSFSALS
                     KPFFYFANSWGLIAGVRWTDRIGKGIRTAPRDALVADSTPNEIRGLAFGLHRAMDHAG
                     AMLGLLIAALAVWLAQKNSLELSRSTFQMIVLLSLIPAVLAVLSLVIGAKDVPVTSQR
                     EVPKFSLRNLGKPFNIFLVIVSIFTLGNSSDGFLVLRAQNLGVSVTGVLVMLAVFNLV
                     VTLVASPAGSLSDRVGRRRLIITGWLVYAAIYFGFGFATSFWHVWLLYLVYGLYFGLA
                     FGTANAMVADLVPENMRGTAYGTYHAVIGVLAFPSSLIAGILWEGVGTWSGFGPSAPF
                     IFGGSMALVAAMLMAFWMPHKLIRKATDGTA"
BASE COUNT          714 a          758 c          671 g          522 t
ORIGIN      
        1 gtattaaaaa ataccgagcc aactggcgat gacctgctcg atgaagccat caacgatatt
       61 caccaggtcg aaaaacctca ccggcccgcg tattggatca gccagttcat cgcaaaaaag
      121 aagaaactgc gcgagcagct gggaattcgc ctggcgagcc agggatacct gcaccaggaa
      181 gataagcgct tcttctggat tttcaaggaa gatgaagctg ataacccgat ggcacccttc
      241 aagtatcagc taaaatcaga tttacgggcg aaaacactca caaccagcgc caatgatgcc
      301 cacagcctgg cgctgctcaa gatcctgact gccagcggcc tgctcggcct ggtcttcaca
      361 gccgatgaac acgccctggc gacgcggacg attaacgaaa aagtaatccg agccgctctg
      421 gaaaccccag agctgcaaac gatcgaggag ataggccagg gaattgcgat tacgatcgaa
      481 gatgaattag attaaagcaa aaatcagata gtttgcacca tccgatcaga taaatcttta
      541 caacatccgc gatcctgcgg atgtttttta taactggttt cgttctctta tttctgggtg
      601 gtaatggaca tgcacaacct ggaggaaatc aaccgtgctt ttgagtttgc gctcagcaag
      661 cgtcgaaatc cggtctcctt cgctgacggt cagcgatcca tccacagcca gggtcagatt
      721 catcattaag tatggaccaa agcggtgagc attaatgtct tcaacgcttt tgatcccact
      781 gatccccgcc agggattctt caacctgctt ctgtaattct tccccgggga cggtatccat
      841 cagctcggag gctgattcac gcaggatctg gatcccggtg cgtaaaatca cgagagcaac
      901 aagtgccccc gccagaggat ctacccacga ataacccatc cggccgagcg taatgccgat
      961 caatgctgct gacgccgaaa aaatatcatt gcggtggtca tacgccaggg ccatgatcgc
     1021 agtattgttc aactgccggg aaaggcggtt ggtatagata aacagaccga ttttcagggc
     1081 gatggtaagc atggcgaccc acagagcgat taaactcgca ccctgggaaa caaccgcacc
     1141 ggtgagctgc tcatagatcg aattcaccga attccagaaa atcccgactg cggtcgtgat
     1201 gacgaaggag ccaaccgtaa gggaggcaat cgcctcgaac tgactgtgcc caaaagggtg
     1261 ttctcgatcg gcgggcttgg acgaaaagcg caggaatatc gctacgacca gataagaaac
     1321 aacatctgag gttgagttga tcccatcggc taagagggcc gggctgctcc ccaggatacc
     1381 caccgctgtt ttaatgatcg ccaggaagat attggctgcc aggccaaaat tgactgccag
     1441 catactcttc ttgtgacgct gaatattttt ttcttcatgc atttcttgat tgacaatact
     1501 ggacatttag tggcgccttc cacaaacaat tctttgcatt ctaccacttt ccgctcaacc
     1561 atccgcctta cgctgtgcca tctgtcgctt tccgaatgag cttatggggc atccagaaag
     1621 ccattaacat ggccgcgacc agagccatgc tcccgccaaa aataaacggt gccgatgggc
     1681 caaaaccact ccaggtgcca accccttccc agaggatgcc ggcgatcagg gatgaaggga
     1741 aagccagcac accgatcacg gcgtggtagg tgccgtaggc agtgccgcgc atattttcgg
     1801 gcaccagatc agcgaccatc gcgttggcgg tgccaaaggc caggccgaaa tacagtccgt
     1861 agaccagata cagcagccag acgtgccaga aactggtggc aaagccgaag ccgaagtaga
     1921 ttgcggcata cacaagccag ccggtgataa ttaaccgccg ccgcccaacc cgatcggaaa
     1981 gcgatccggc aggcgaagcc accagagtca ccaccagatt gaataccgcc agcataacca
     2041 gcacgccggt tactgagaca cccagattct gtgctctcag caccaggaat ccatccgagg
     2101 aattccccag cgtaaagatg cttacgatta ccaggaagat gttgaatggt ttgcccagat
     2161 tacggagcga aaactttggg acctctcgct ggctggtaac gggcacatcc tttgctccaa
     2221 tcaccaggga taataccgcc aggacggccg ggatcaggct caataatacg atcatctgaa
     2281 acgtcgatcg ggagagctca aggctgtttt tctgagccaa ccacaccgcc agcgcggcga
     2341 tcaacaagcc cagcatcgcc ccagcatgat ccatcgcccg gtgcaggcca aacgccaggc
     2401 cgcggatctc gttgggggtc gaatccgcca ccagggcatc ccgcggtgca gtccggatac
     2461 cctttccgat ccgatctgtc cagcgcaccc cggcgatgag cccccagctg tttgcaaaat
     2521 agaaaaaggg cttggacaac gccgagaacg aataaccggc aacggccagc cacttacgcg
     2581 cacccagttt gtcggagagc cagcccgaaa acagcttcaa gatgctggca gtggcctcag
     2641 ccaccccttc aattaagcca ataat
//
LOCUS       DATMXK010000527         2247 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_75216, whole genome shotgun sequence.
ACCESSION   DATMXK010000527 DATMXK010000000
VERSION     DATMXK010000527.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2247)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2247)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2247
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75216"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..958
                     /locus_tag="VN363_09570"
     CDS             <1..958
                     /locus_tag="VN363_09570"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWQ84805.1"
                     /translation="FTGCGSESDNLALRGAAFAARQQRGANHILISPVEHHAVSHTAG
                     QLASLHGFELETLPVDEYGMVHPEAVAERLKPDTAVVSVMYANNEIGTINPIAEIGAL
                     CREAGVAFHTDAVQAAAYLPVDVEALKVDLLALGAHKFYGPKGVGALYIRKGTSLTPM
                     LTGGAQEFGLRAGTHNIPYIAGMAEAFRLAQVERQERTAHLQPLRDHLIGAVLEAIPD
                     SRLTGHPTDRLPNNASFVFEGVDGNALLALLDVEGFACSSGSACKTGDPEPSGVLLAL
                     GLDRKWALGSLRVTLGVSTTPLEIDAFLNVLPEAVRRVRSLS"
     gene            969..2081
                     /gene="mnmA"
                     /locus_tag="VN363_09575"
     CDS             969..2081
                     /gene="mnmA"
                     /locus_tag="VN363_09575"
                     /EC_number="2.8.1.13"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559884.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016740 - transferase activity [Evidence
                     IEA];
                     GO_function: GO:0016783 - sulfurtransferase activity
                     [Evidence IEA];
                     GO_process: GO:0008033 - tRNA processing [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA 2-thiouridine(34) synthase MnmA"
                     /protein_id="HWQ84806.1"
                     /translation="MSAESKRVVVAMSGGVDSSVAAALLQQQGYQVIGMMLRLWNEPG
                     GESYNRCCTPDSMAQARRVAAILGIPFYAVDGQEAFYQQVVIPFLDGYTQNTTPNPCA
                     FCNRHVRWEFLLQRALTFGASYMATGHYVRLQRDADGRAHLHQALDPQKDQSYILHVL
                     GQDQLKYALFPLGEYSKPEIRQLARDFKLPVSERSDSQDLCFLGQSDLRGFLQRHAPA
                     SMVPGPITDRQGRVLGQHQGLASYTIGQRKGLGIAAPRPLYVLARRPVDNTLVVGEKS
                     ELGCSSLITHPVHWISGAAPATPFQASLKIRSTAPLTPAWIQPLPDGGMLADFAQPLR
                     DITPGQAAVIYLDGEVLGGGIIKAAGSGQNSGEDNA"
BASE COUNT          376 a          761 c          662 g          448 t
ORIGIN      
        1 ctttaccggc tgcggctcgg agtccgacaa cctggccctg cgtggggctg cctttgccgc
       61 ccgccagcag cggggcgcca accatatcct gatctcgcca gtcgaacacc acgccgtcag
      121 ccacaccgcc gggcagctcg ccagcctgca cggctttgag ctggaaaccc tgcccgtgga
      181 cgagtacggc atggtccacc cggaagcagt cgccgagcgt ctcaagccgg atactgcggt
      241 cgtttcggtg atgtatgcca acaatgagat cggtaccatc aatcccattg ccgagattgg
      301 cgctctgtgc cgggaggccg gggtcgcatt tcacaccgat gctgtccagg ctgccgccta
      361 cctgcctgtc gatgtcgaag ccttgaaagt agatctgctc gctctcggtg cgcacaaatt
      421 ctacggcccc aagggtgtgg gcgctctgta tatccgcaaa ggcacctcgc taacgcccat
      481 gctcaccggg ggcgcccagg aattcgggct gcgcgccggc actcacaata tcccctatat
      541 tgccggtatg gcagaggctt ttcgcctggc tcaggtcgaa cgccaggagc gcaccgccca
      601 cctccagccg ctgcgcgacc acctgatcgg cgcggtcctc gaagccattc ccgactcgcg
      661 cctgaccggg caccccaccg accgcctgcc gaataacgcc agcttcgtct ttgaaggcgt
      721 ggatggcaat gccctgctgg cgctgctgga cgtggaaggt tttgcctgct cgtccggctc
      781 cgcctgtaag accggcgacc cggagccatc cggggtcctg ctcgcccttg gtttagaccg
      841 aaaatgggcg cttggctcgc tgcgggtcac cctgggcgtt tccaccaccc ctctcgagat
      901 cgacgctttc ctgaacgtgc tgcctgaggc ggttcgccgc gtccgcagtc tgagctgagg
      961 aacccgaatt gagcgcagaa tcgaagcggg tagtcgtcgc catgagcggc ggagtcgaca
     1021 gctcggtcgc ggccgccctg ctccagcagc agggctacca ggtgatcggg atgatgctcc
     1081 gcctgtggaa cgagcctggc ggtgaaagct acaaccgctg ctgcacgccc gattccatgg
     1141 ctcaggcccg ccgcgtggca gccattctcg gtatcccatt ctatgccgta gatgggcagg
     1201 aagcctttta ccagcaggtc gtgattcctt ttcttgatgg ctacacgcag aataccaccc
     1261 cgaatccttg cgccttctgc aaccgccatg tccgctggga gttcctgctg cagcgcgccc
     1321 tcaccttcgg tgccagttat atggctaccg gccactatgt ccgcctgcag cgcgatgcag
     1381 acggccgggc tcatctgcac caggccctgg accctcagaa ggaccagtct tatatcctcc
     1441 acgtcctggg tcaggaccag ctcaaatacg cccttttccc gctcggtgaa tattccaagc
     1501 ccgagatccg ccagctggcc cgcgatttca agctgcccgt ctccgagcgc tccgactcgc
     1561 aagatctgtg cttcctgggg cagagcgatc tgcgcggctt cctgcagcgc catgctcccg
     1621 ccagtatggt acccgggccc atcaccgacc ggcaggggcg tgttttaggc cagcatcaag
     1681 gcctggcctc ctacacaatc ggccagcgca agggattggg catcgcggcc ccccggccgc
     1741 tgtacgtgct cgccaggcgc cctgtcgaca acaccctggt tgtcggcgag aaatccgagc
     1801 tgggctgctc cagcctcatc acccatccag tgcattggat cagcggcgca gcgccagcga
     1861 caccattcca ggcgtcatta aagattcgct ctaccgcccc ccttaccccg gcctggatcc
     1921 agccgctgcc ggatggtggg atgctggcag atttcgccca gccgctgcgc gatataaccc
     1981 ccgggcaggc cgcggtcatt tatctggacg gtgaagtgct gggtggtggt atcatcaagg
     2041 ctgctggcag cggccaaaat tccggggagg ataacgcatg aatctacctt cattcatcct
     2101 gggcgcatta attgccacac tgtatggggc agggtttcac ctggcgcgtg ggggcgggct
     2161 ggggcacttg ctgctctatc ttggtctcgc ctggctgggt ttctggtccg ggcatttcat
     2221 cgccgcccag ttcggctgga ctttctt
//
LOCUS       DATMXK010000528         2541 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_84861, whole genome shotgun sequence.
ACCESSION   DATMXK010000528 DATMXK010000000
VERSION     DATMXK010000528.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2541)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2541)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2541
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84861"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(91..1167)
                     /locus_tag="VN363_09580"
     CDS             complement(91..1167)
                     /locus_tag="VN363_09580"
                     /inference="COORDINATES: protein motif:HMM:NF038032.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CehA/McbA family metallohydrolase"
                     /protein_id="HWQ84807.1"
                     /translation="MESSTYELVINLHMHTPYSDGSGSHAEIARAALRAGLDAVIVTD
                     HNVWVNGPQGYYQEGDRRTLLLVGEEVHNRTLQPQKNHLLVFGAYKEMAGYAEDLQRL
                     IDAVRTAGGLAFIAHPVDPAAPTFREVDISWEDWSVQRFTGLELWNGMSEFKGRLKSW
                     LHGLYYAFNPRQIAHGPFPQAIQRWDSLLAEKKKVVAVGGSDAHELHYRIGPFHKVLF
                     PYEFHFKAINTHLLADEPLSGEALEDSTLILETLRKGRVFIGYDLPASTRGFRFSAQG
                     SEGTAQMGDQLTAKGGVTFQIRLPMRTECRLIKDGSPIKTWQQNELCTHIATEPGVYR
                     VEVYIQFAGRRRTWIISNPIYVVK"
     gene            1239..1463
                     /gene="secG"
                     /locus_tag="VN363_09585"
     CDS             1239..1463
                     /gene="secG"
                     /locus_tag="VN363_09585"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559553.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005886 - plasma membrane [Evidence IEA];
                     GO_function: GO:0015450 - protein-transporting ATPase
                     activity [Evidence IEA];
                     GO_process: GO:0043952 - protein transport by the Sec
                     complex [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="preprotein translocase subunit SecG"
                     /protein_id="HWQ84808.1"
                     /translation="METYLDIALIITSIALIASVILQSKGAGLGGLTGGDTGGIFTAR
                     RGIEKVLFRITIVLAVLFFALAIATVLVRG"
     gene            1603..2295
                     /locus_tag="VN363_09590"
     CDS             1603..2295
                     /locus_tag="VN363_09590"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ84809.1"
                     /translation="MKRLRWQLVVVVITLGVILILLLSQQPGVLPGIVEPVVQPTSGG
                     AYSEALVGSLRRLNPMLDWYNQPDKDVDRLLYCSLMRFDERAAPLGEVVETYGISADG
                     TVYNFSIRENAVWHDGEPVTSDDIVYTTGLLADPDLPAPEDLKAIWADIDVVPLDDKT
                     FQMVLPEPFGPFLDFLAVQAQEQRLAQAGILQERQLGIVQIEQYQLQARQASGVQVQI
                     DFFEALIQGLGP"
BASE COUNT          506 a          707 c          750 g          578 t
ORIGIN      
        1 cctttttgga tgccctcgac cagctggcgg attgcttctg gctggtcgcc ggtcgggata
       61 aaaggggctt tgagttggaa ttccatagac ttatttcacc acatagatcg ggttgctgat
      121 gatccaggtt cgccgccgtc cggcaaactg gatatagact tcgacccggt atacgcccgg
      181 ctcggtggct atatgggtgc acagctcgtt ctgctgccac gtcttgatgg gcgagccatc
      241 cttgatcagg cggcactctg tgcgcatcgg caggcggatc tgaaaggtaa ccccgccttt
      301 agcggtaagc tggtcgccca tctgggcggt cccttcgctg ccctgggcgc taaatctgaa
      361 tccgcgcgtg ctggcaggca aatcataacc gataaacacc cgccctttgc gcaaagtttc
      421 caggatcagg gtagagtctt ccagggcttc tccgctcaag ggttcatcgg ccagcaggtg
      481 ggtattgatg gctttgaaat gaaactcgta tggaaagagc actttgtgga atggtccaat
      541 tcgatagtgg agctcatgtg cgtccgagcc gcccacggcg accacctttt ttttctcggc
      601 cagcaggctg tcccagcgct ggatggcttg cgggaatggt ccatgggcga tctggcgggg
      661 attgaatgca taatacaggc catgcagcca gcttttcagc cggcctttga actcagacat
      721 gccgttccag agctccaggc cagtgaagcg ctggacgctc caatcttccc aggagatatc
      781 cacctcccgg aaagtggggg cagccgggtc gaccgggtga gcgataaaag ccagcccgcc
      841 cgctgtgcgc accgcgtcga tcaagcgctg caggtcttcg gcatagccag ccatttcttt
      901 ataggcccca aacaccagca ggtgattttt ctgtggctgg agggtccggt tatggacttc
      961 ttcaccgact aacaacagcg tgcgccgatc cccctcctgg taatagccct gcggcccatt
     1021 cacccaaacg ttgtgatcgg tgacaatgac cgcgtccaac ccggcgcgca gcgcagcccg
     1081 ggcgatctcg gcgtggctgc cgctgccgtc cgaatagggc gtatgcatat gtaaattgat
     1141 caccagctcg taggtggaag actccatttg acgtttcgct tgtggaaagg tataatccgc
     1201 ctgcctgaaa gcatgattca aacgtttgga gcataatttt ggaaacttac ctggatatcg
     1261 cactgattat aacgtcaatc gccctgatcg ccagcgtcat cttgcaaagc aagggcgccg
     1321 ggttgggcgg tctgactggc ggcgacaccg gcggaatttt cacagcccgt cgcgggatcg
     1381 agaaagttct gttccggatt accatcgtct tggccgtcct cttcttcgca ttggcgattg
     1441 ccactgtatt ggtgcgagga tagctctgcc gggggaaccc gtttgatttg aaaggaaaag
     1501 gataggcagc aggaaagaag ccaggggctg ttctcggctg gtcgtctggc gacaggccgg
     1561 tgagcgccct tttttgttgt ctggcatcct ttgaatttgc tcatgaaaag attgcgctgg
     1621 caacttgtgg ttgttgtcat cactctgggg gtgatcctta tcctgctgct cagccagcag
     1681 ccgggggtgc tgccggggat cgtggagcca gtggtccaac ccacctccgg cggcgcgtac
     1741 agcgaagcgc tggtcggctc gctgcggcgc ctgaatccca tgttggattg gtacaaccag
     1801 cccgataaag atgtcgaccg cctgctctac tgcagcctga tgcgctttga tgagcgcgcc
     1861 gctccgctgg gtgaagtcgt agaaacttac ggcatctcgg cagatggtac ggtctataac
     1921 ttttccattc gcgaaaatgc tgtctggcac gatggcgagc cggttaccag tgatgacatt
     1981 gtctatacca cgggcttgct ggccgatcca gatctgcctg cccctgaaga cctgaaagcc
     2041 atatgggcgg atatcgatgt cgtccctctg gacgacaaga ccttccagat ggttctgcca
     2101 gagccttttg gaccgttcct cgattttctg gcggttcagg cccaggagca gcgcctggcg
     2161 caggctggga tcctgcaaga aaggcagctc ggcatcgttc agattgaaca atatcagctc
     2221 caggcgcggc aggcgagcgg tgtacaggtt caaattgact tcttcgaggc cctgatccag
     2281 ggtctcggcc cctaattcgc ttacccccag cacttcttcg cgctggtaag cagtcagggc
     2341 tgcggcagca tcggggtagg tcttaaatgt gacttcatcg atataggcag gctgggcaaa
     2401 atagtcgggg aaggatttaa gcaccacgcc attgatcgcg cccgcctcgg ccaggaaatg
     2461 atcgaagcgg aatgggccgc agccgaccgg gttcaaattg aaatcggcgt tcaccagatc
     2521 ggccgcgccg gtgccatcca g
//
LOCUS       DATMXK010000529         2054 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_28474, whole genome shotgun sequence.
ACCESSION   DATMXK010000529 DATMXK010000000
VERSION     DATMXK010000529.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2054)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2054)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2054
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_28474"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..>2054
                     /locus_tag="VN363_09595"
     CDS             <1..>2054
                     /locus_tag="VN363_09595"
                     /inference="COORDINATES: protein motif:HMM:NF024816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ84810.1"
                     /translation="APANARLARQQISLAALPQQDSRDLVLEILRKVDDLPPALLDLI
                     TTRAEGNPFFIEELIKMLIEQQVILKDGERWRVDLTQLADVQIPSTLVEVLQARLDSL
                     SPEERVLMQRAAVVGRVFWDAAVSAMEKEPAELANLEGAQKGASQIGDQTRIAPQLAE
                     IIHNLGAKEMVFARPESSFAETQEYYFKHALLRDVTYNNVLKRLRKVYHAYAAAWLEA
                     ISEQSRRSGEYAGLIAEHYHRADDHAHARAWYQRAGELAADTYANSEAVRCLTHCLEL
                     TPADDPVGKFALLLRRAKVYDTLANRPAQKADLEALTALAEQLDAGAPVEAGAHAAYS
                     YRALIYLQWWFYHDQMGDSQAAMAAARQAKDLAAAAGDLESEALAYLYLGGSLWRRSE
                     FPQAEVVLNKSMALARSLQLPNLEADSLRQLGIAHQYQGDFSAARQAYESAILVYNTT
                     GNDKGQSMALNSLGSLAVDQGLYQEGLAYYQRSLELKRKIGHRYGEHITLFNMGALAD
                     RMGSYLEAIGYLEKVEQFGLETGSKEEEADALNGLGGVYLHLGDYATAGACLERALIL
                     GRELDNESTVWDALQGLGILAHLQGDDASAERLGQEALALARKHNLALMETNSLFNLG
                     LAVLALGHTGEALEHFQHALELAQADTDPRPRIEIQAGLAEVRLAETRLAEARLAEAR
                     LAG"
BASE COUNT          400 a          655 c          665 g          334 t
ORIGIN      
        1 gcgccggcca atgcgcggct ggcccggcag cagatatccc tcgccgccct gccgcagcag
       61 gacagccgcg atctggtgct ggagatcctg cgtaaagtgg acgatctccc gccagctttg
      121 ctggatttga tcaccacccg cgccgaaggt aacccgttct tcatcgaaga gctgatcaag
      181 atgctcatcg aacagcaggt gatccttaaa gacggcgagc gctggcgggt ggacctgacc
      241 cagctggcgg atgtgcagat cccgtcaacc ctggtcgaag tcctgcaggc tcgcctcgac
      301 agcctgagcc cggaggagcg ggtgctcatg cagcgggcgg cggtggtcgg cagggttttc
      361 tgggatgcgg cggtgagcgc catggaaaaa gagccggcgg agctcgccaa tctcgagggc
      421 gcccagaagg gagcctccca gatcggcgac cagacccgga tcgccccaca gctggcggag
      481 attatccaca acctgggcgc taaggagatg gtgttcgcgc gcccggaatc gagtttcgcc
      541 gagacccagg aatactattt caagcacgcc ctgctgcggg acgtgactta caacaacgta
      601 ctcaagcggc tgcgcaaggt ctaccatgcc tatgcggctg cctggctgga ggcgatttct
      661 gagcaaagcc ggcgctccgg cgaatacgcc gggctgatcg ccgagcatta ccaccgcgca
      721 gacgaccatg cccatgcccg cgcctggtac cagcgcgccg gggagctggc tgcggatacc
      781 tacgccaaca gcgaggcggt gcgctgcctg acccactgcc tggagctgac tcccgcagac
      841 gatccggtgg ggaaatttgc gctcctcctg cggcgggcta aagtctacga taccctggct
      901 aaccgcccgg cccaaaaagc cgacctggaa gcccttaccg ccctggccga gcagctggat
      961 gccggagctc cagtggaggc tggggcgcat gcggcctact cctaccgggc actcatctac
     1021 ctgcagtggt ggttctacca cgatcaaatg ggcgattcgc aggccgcgat ggcagccgcc
     1081 cgccaggcta aagacctcgc cgctgccgcg ggtgacctgg aaagtgaagc cctggcatac
     1141 ctgtatctgg gaggctcgct ctggcggcgg tccgagttcc cgcaagcaga ggtggtgctc
     1201 aataaatcca tggcgctggc gcgcagcctg cagctgccca atctcgaagc ggactctctg
     1261 cgccagctgg gaattgccca ccaataccag ggcgatttca gcgccgcccg ccaggcatac
     1321 gaatccgcaa tcctcgttta caacactacc ggcaatgata aaggccagag catggccctg
     1381 aacagcctgg gctcgctggc tgtggaccag ggcctctacc aggaagggct ggcttattac
     1441 cagcgctcgc tcgaattgaa acgcaagatc ggccaccgct acggtgagca catcaccctg
     1501 ttcaatatgg gcgccctggc cgaccggatg ggcagctatc tggaggcgat cggctacctg
     1561 gaaaaggtcg agcagtttgg cctcgaaacc ggcagcaagg aagaagaagc cgatgccctg
     1621 aacggcctcg gcggcgtgta cctgcacctg ggagattacg ccactgccgg agcctgcctg
     1681 gagcgcgccc tgatcctggg gcgggagctg gacaatgaga gcaccgtctg ggatgcgctg
     1741 caggggctgg gaatcctggc gcatttgcag ggagacgacg cttctgccga gcggctgggg
     1801 caggaagccc tggccctggc gcgcaagcac aacctggcac tgatggaaac caacagcctg
     1861 tttaacctgg ggctggctgt actcgccctg gggcacaccg gcgaagctct ggaacatttt
     1921 cagcacgccc tggagctggc gcaggcagac accgaccccc gcccgcggat cgagattcaa
     1981 gccggtcttg ccgaggtacg gcttgccgaa acccggcttg cggaggctcg gcttgcggag
     2041 gctcggcttg ccgg
//
LOCUS       DATMXK010000530         2769 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_17791, whole genome shotgun sequence.
ACCESSION   DATMXK010000530 DATMXK010000000
VERSION     DATMXK010000530.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2769)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2769)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2769
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17791"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..214)
                     /locus_tag="VN363_09600"
     CDS             complement(<1..214)
                     /locus_tag="VN363_09600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560469.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84811.1"
                     /translation="MPIDEITPIRKQYLEIKRQYPHAIVFFRLGDFYETFDEDAEITS
                     RELDIVLTSRNVAKGARVPMAGIPYHA"
     gene            366..1466
                     /gene="ugpC"
                     /locus_tag="VN363_09605"
     CDS             366..1466
                     /gene="ugpC"
                     /locus_tag="VN363_09605"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_016292962.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="sn-glycerol-3-phosphate ABC transporter
                     ATP-binding protein UgpC"
                     /protein_id="HWQ84812.1"
                     /translation="MASVTFDHVYKRFGDVIAVNDLNIQVEDQEFLVLVGPSGCGKTT
                     ALRCLAGLEDISDGRILISDRVVNDVAPKDRDIAMVFQSYALYPHMSVFDNMAFGLKL
                     RKTPREEIKRRVGAAADVLGIGALLDRKPRQLSGGQRQRVAVGRAIVREPKVFLFDEP
                     LSNLDAKLRVETRANISKLHQQLKTTFIYVTHDQVEAMTMASRIAVITKGILQQIDTP
                     QHLYDHPDNLFVAGFIGSPAMNFFAATLRKEGEKLFVDGDSFTVEIPADKVPIYMPLA
                     GKPVIFGIRPEDIHNPQFAPPGITTQPVEAAVDVTELMGNEIFVYLKTGNHSYVARVD
                     PRTRFHMGDKVQMVFNMDNFHIFDKDTERAVR"
     gene            1551..2297
                     /gene="gpmA"
                     /locus_tag="VN363_09610"
     CDS             1551..2297
                     /gene="gpmA"
                     /locus_tag="VN363_09610"
                     /EC_number="5.4.2.11"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560674.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004619 - phosphoglycerate mutase activity
                     [Evidence IEA];
                     GO_process: GO:0006096 - glycolytic process [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="2,3-diphosphoglycerate-dependent
                     phosphoglycerate mutase"
                     /protein_id="HWQ84813.1"
                     /translation="MYKLVLLRHGQSTWNLENRFTGWTDVGLSDQGVQEAIGAGRILK
                     QEGYTFDIAFTSVLKRAIKTLWLVMEEMDLEWMPVVRAWQLNERHYGALQGLNKAEMA
                     EKYGDAQVKVWRRSYATPPPPLEKDDPRYPGFDRRYARLTDAELPQAEALKNTVERML
                     PYWHTTISPIVQQGKRVLIAAHGNSLRALVKYLDNISDEAIPELNIPTGVPLVYELDE
                     DLKPLKSYYLGDPEAIQKAAEAVANQGKAK"
     gene            2578..>2769
                     /locus_tag="VN363_09615"
     CDS             2578..>2769
                     /locus_tag="VN363_09615"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84814.1"
                     /translation="MTVPNQTNLAETPVQASRSWLISLVGFLLVAEAVFLIVLFPALI
                     LLRLWQLPDLSPSQLLPLQK"
BASE COUNT          648 a          776 c          744 g          601 t
ORIGIN      
        1 cggcgtggta gggtataccc gccatgggca cccgggcgcc cttggcgaca ttgcgcgagg
       61 tgagcacaat atccagctcc cgcgaggtga tttcggcgtc ttcatcaaaa gtctcataaa
      121 aatcgcccag gcggaagaac acgatcgcat gggggtattg gcgtttgatc tctaaatatt
      181 gtttacggat cggggttatt tcatcaattg gcatgcccca attctatatt caggcaggcg
      241 aaacggcaag cacctggagc aaagtcataa gcgaataatc atagactgct aacgctctgg
      301 attgctttta aagtaaggcg cgcatataat taagccacta tactcgttcg gaaaggagac
      361 ttattatggc aagcgtaacc ttcgatcatg tgtacaagcg tttcggggat gtcatcgcgg
      421 tcaatgacct gaacatacag gttgaagacc aggaatttct ggtgctggtc ggtccttcag
      481 gttgtggaaa aaccactgcc ttgcggtgcc tggccggcct ggaagacatc tcagatgggc
      541 gcatcttaat cagcgaccgg gtggtcaacg acgtcgcgcc caaagaccgg gacatcgcaa
      601 tggtgttcca gtcctatgca ctctaccccc atatgagtgt gttcgacaac atggctttcg
      661 gtcttaaact gcgcaagacc cccagggaag agatcaagcg ccgggtcggt gcggcagccg
      721 atgtattggg catcggggca ttgttggatc gcaaaccccg ccagctctcc ggtggtcagc
      781 gccagcgggt ggcagtcggg cgggcgatcg tgcgcgagcc aaaagtcttc ctgttcgatg
      841 agccgctctc caacctggac gccaagctgc gggtcgaaac ccgcgccaat atcagcaagc
      901 tgcaccagca gctgaaaacc acctttatct acgtcaccca cgaccaggtc gaagccatga
      961 ccatggcgtc gcgcattgcg gtgatcacga agggcatctt gcagcagatc gacacacccc
     1021 agcatctgta tgaccacccc gacaacctct tcgttgctgg ctttatcggc tcgccggcga
     1081 tgaacttctt tgctgcaacg ctgcgcaagg aaggtgaaaa actattcgtc gatggcgatt
     1141 ctttcacagt agagatcccc gctgacaagg ttcccatcta catgccgctg gctggcaaac
     1201 cggtcatctt tggcattcgc cctgaggata tccacaaccc gcagttcgcc cctcccggca
     1261 tcaccaccca acctgtcgaa gccgctgtgg atgtcaccga attgatgggc aatgaaatct
     1321 ttgtgtatct gaaaaccggc aaccacagct atgtcgcccg ggtagacccc cgcactcgct
     1381 tccacatggg cgataaagtg cagatggtct tcaatatgga caatttccac attttcgaca
     1441 aggataccga acgcgccgtt cgctgagcag cctccggtaa gccgatttga tctggcgtct
     1501 cccttcgagc acacttgccc ggggagacgc cttcatgaaa aggagcaacg atgtataaat
     1561 tggtgttgtt acgccacgga cagagcacat ggaacctcga aaaccgtttt accggctgga
     1621 ccgacgtggg tctgtcggat caaggcgtcc aggaagcgat tggtgccggg cggatcttga
     1681 agcaggaagg ctatacattt gatatcgcat tcacttcggt ccttaaacgc gccattaaga
     1741 ccctgtggct ggtcatggaa gagatggatc tggaatggat gccggtggtg cgcgcctggc
     1801 agctcaatga gcgccattat ggtgcactgc agggcttgaa taaagctgag atggctgaaa
     1861 aatacggcga cgcgcaggtc aaggtctggc ggcgcagtta tgcaacccca ccgccgcccc
     1921 tggagaagga cgatccgcgc tacccgggtt tcgatcgccg ttacgcccgc ctgacggacg
     1981 ccgagctgcc ccaggcggaa gctctcaaaa acactgtgga gcggatgctg ccatactggc
     2041 atacaaccat ctcaccgatt gtccaacagg gcaagcgcgt cctgattgca gcacatggca
     2101 acagcctgcg cgccctggtg aaatatctgg acaatatctc ggatgaagcc atccccgagc
     2161 tgaacatccc taccggtgtg ccgctggtat atgagctgga tgaggacctt aaaccgctaa
     2221 agagctacta cctgggcgat ccggaagcca tccagaaggc tgccgaggca gtcgccaacc
     2281 agggaaaagc caagtaaaga cactcaaaat ctccggttcg gttgtgcgtt ttggcgaacg
     2341 tttgccagac ccagcgtaac cagaggcata aaacgagctg ggtgtatttt aacttcagaa
     2401 caagctgcat ttccaggctg aggctgcccg tgtagtatgg gcagcctcag cttttcatac
     2461 cccttaccac ttgtaactaa tggctataga atttcgcaat ctattccaga gggcagcctt
     2521 tcggttacaa tcatgctgat tcagatccaa gcccacatct gcggggttgc aacgtttatg
     2581 acagtcccaa accaaaccaa tcttgccgag acccccgttc aggccagccg ctcctggctg
     2641 atcagcctgg ttggtttttt gctggtggct gaagcagtct tcctgatcgt cctgttccct
     2701 gccttgatcc tgttaaggct gtggcagctg ccggatctat cgccaagcca actgctgcct
     2761 ctccaaaag
//
LOCUS       DATMXK010000531         7080 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_84881, whole genome shotgun sequence.
ACCESSION   DATMXK010000531 DATMXK010000000
VERSION     DATMXK010000531.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7080)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7080)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7080
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_84881"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..276)
                     /locus_tag="VN363_09620"
     CDS             complement(<1..276)
                     /locus_tag="VN363_09620"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84815.1"
                     /translation="MDYSLLPTQVILGALLLAWLGTCVFFDLRSRQVPTPLTVIPLIL
                     AAAWRLFQGNWQLVLLTVVLILLSDLPWAKWRVPFGCTAAIVGLSIAG"
     gene            complement(246..1427)
                     /locus_tag="VN363_09625"
     CDS             complement(246..1427)
                     /locus_tag="VN363_09625"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84816.1"
                     /translation="MEIRSLYLLSYFFSIAFVMAWLARLGLDFYHWSRKAGLYRLLAG
                     QRISLKPLRDKLPWNVLLLSVSGLLFGWVVISWIEAWFVREWLLLILPALAILCIELL
                     LTKKDISLLAVLALIAGLRGQREAGQDFFERLIKVVDGLPPGEIQTACRESIQRRRSG
                     LPVDQSCQALSGLHPILNELVFTLRLTGWQASPAFDLSLERLAQRAGKQWDRLSRWMV
                     FREQVQPILLFSQMAILAALLYLVVEGIPAFTLAWPSYDVIAWIGLGCILAAGMLYAT
                     HYRTWLRRLVGSALLIASFVPLWQYASLPRLFELQLHSATQISERIQAKQTGWEAIQA
                     PTAKPKFLDTVPEEQDNTLLSTDTPAYQSMVDLRAQPSSISSQNYADDHSWTIPCYQP
                     R"
     gene            complement(1427..1987)
                     /locus_tag="VN363_09630"
     CDS             complement(1427..1987)
                     /locus_tag="VN363_09630"
                     /inference="COORDINATES: protein motif:HMM:NF016107.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004671 - protein C-terminal
                     S-isoprenylcysteine carboxyl O-methyltransferase activity
                     [Evidence IEA];
                     GO_process: GO:0006481 - C-terminal protein methylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="isoprenylcysteine carboxylmethyltransferase
                     family protein"
                     /protein_id="HWQ84817.1"
                     /translation="MKDRLGLAFFTFAAVISALAAWEHPSILAWLAALHNAILAGLYA
                     RRKPAREYDRRGLWLGVLAAILPIAAPYPEQVPLVLLFVGVSGYVLVLWSLLTLGARF
                     GIAPADRGLVANGPYRFVRHPMYLGELVLRLALVSTSQQPFLAAGLFIALAAIQILRS
                     LREELIISGYLAYAHQVRFRLIPGVW"
     gene            complement(1994..2140)
                     /locus_tag="VN363_09635"
     CDS             complement(1994..2140)
                     /locus_tag="VN363_09635"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84818.1"
                     /translation="MSDYGITTAQALSIAGSTFAFGFVFGWIWALFHFGGSSSHSNHT
                     DKED"
     gene            complement(2175..3032)
                     /locus_tag="VN363_09640"
     CDS             complement(2175..3032)
                     /locus_tag="VN363_09640"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84819.1"
                     /translation="MTITYLIGIATAALLLTLWVELVPSLPAGKAARFLRESDQPAAP
                     ELPGWRKWLSKLDRSTARWTPASILRRAKADLYFAQLGGKWEGWDEVQFTSLRIALCL
                     GAFVFGLLVYDNMIPSLLASLLGWQVPVVMLGGVARRVRRRFQSQLPEFIQLVAAQMA
                     AGVSLEEALRRTSQTESLAAKWMRHVLQMSQGRVIFTQLQKEAQASYLPDLIGLAVQL
                     EFVRLGTAQQELMGQMAGRIAAEYAAGAEQRAERVGAELVIPMVIFYFLPFITVILVL
                     IGWPILATL"
     gene            complement(3029..3913)
                     /locus_tag="VN363_09645"
     CDS             complement(3029..3913)
                     /locus_tag="VN363_09645"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84820.1"
                     /translation="MITLIGILVGLLVFVLFSPARIRHGEASQRLAEMYSLDTARTLS
                     ELKPETLEYKLLAAGLRLQPFTFRLLTIAGGIAVGVITWPMLPGLPALILAIISAYIP
                     YAWLDDRVKSRGREIDRLLPVAVGRITAGLLAGGSVPEVLQKTGESLDMESPNPLTPE
                     LMLTAAELRSKERQQALRSLADRSPSTSLSNLAYLLDGYSEAGGGKYAEVLMQISQRV
                     QQILVSRNRAVAKAGDALLSARIIPVVLLIVFIFLTRDPLIRGSLLAFPVQAVIAMGI
                     GLMVIGYLLIRSIVAEAV"
     gene            complement(3910..5259)
                     /locus_tag="VN363_09650"
     CDS             complement(3910..5259)
                     /locus_tag="VN363_09650"
                     /inference="COORDINATES: protein motif:HMM:NF012652.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATPase, T2SS/T4P/T4SS family"
                     /protein_id="HWQ84821.1"
                     /translation="MFGNSNGKGLSSLYDVARGTERLVDEGLKDTVANEVIDQINREF
                     PVNVLRSPTDTERQRIQERINTLVGQAFRRSNAYSGYQTDGALAAELGRRLMGLGFLD
                     LLLPPARTDLSEICVYSSGLVQVMKKNSVRWETLQDVKPDPHEIIRVLDRILGPQNKS
                     LNEANPTVNAKLPATLHNPGGGRIKALHPVIAPPGRNPSINIRLFEQKPVLPEWILER
                     EMMSPEMMAILQIAMEQGYRILITGGTRTGKTTLLSALCNYLPSAWRIVKIEDPEEIW
                     IERSTVQTIEARPAALGTEIQPYTLANAVDDALRMAPDYLILGEVRDGHAGLSLFRAM
                     MTGHSGACTFHADNPREAVRRLTTVLGADAGVGATDAVQLIAEAVDLLVQIGIRDEVR
                     RVTVIANLAKELSNGDIHFELIYRFDERSLGSQPRWEKVGELRPRILAETSFQEVRL"
     gene            complement(5287..6597)
                     /locus_tag="VN363_09655"
     CDS             complement(5287..6597)
                     /locus_tag="VN363_09655"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84822.1"
                     /translation="MSNAPIADQKVSVMLAGPGHETVFYQMQPPFLSDSRFVISAHAT
                     QWTTFEQSLAQMRPDLVVVQVEIAPGPEALVQVLAEIQVWHGVAILVLPPSIRDVRGI
                     FERAAVVRGVYIAPVNWGEIAQSGYAAVMTERARAAATAPLQQAYLSRSSSAIVGTRV
                     VAFISATGGTGRSTLAESLAYELNVRMNVHSLLMSFDLPPSVVPHLKMRYVPNSMEYF
                     ARPGDGFAASIQTREGLDVLMSPENSVDYQKAAEYSTAHKAEPDSIYSLVMASWTRNY
                     AAVLLDLPAGEQPWSLQGIAAANTAVIVSRCTLADMAAARHTLILLLERLVGEHRIPR
                     EAIYLVLNQVTDRSPISLRGFHDELVGSYGWAPPVAAAIPYNPAISQAQDEQIPAVTR
                     VDDLAKGVRSLADFLFPGGLMAGVNGRDGHSTKSRLRLPRFRFT"
     gene            complement(6601..>7080)
                     /locus_tag="VN363_09660"
     CDS             complement(6601..>7080)
                     /locus_tag="VN363_09660"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84823.1"
                     /translation="VLKSVSSAPIPINASFLPEPTAAALTGLPTPTPTPTPTPAPPMA
                     PLARIAISGLKVLMVPQSFRYEELPSGSTEEQLFASARTVSAAQEGSVVVLDIPATPI
                     ELVPGLQVNPTTLIVALSQYGSLYLSLEPAGGFEAPDILTLNLADLYDAMNEDRGGK"
BASE COUNT         1720 a         2046 c         1949 g         1365 t
ORIGIN      
        1 tcccgcaata gataacccga caatggctgc tgtgcagcca aatggaacgc gccacttcgc
       61 ccatggtaaa tcggagagaa ggattaagac taccgtaagt aataccagct gccagttgcc
      121 ctggaataac cgccaggctg ctgccaggat cagcgggatg accgtcagcg gtgttggcac
      181 ctgtcgggag cgtaggtcga agaacacgca ggtccccaac caggcgagca agagagcgcc
      241 caggatcacc tgggttggta gcaaggaata gtccatgaat gatcatccgc atagttttgg
      301 gatgagatcg atgaaggttg cgctcgaaga tcaaccatac tttgataagc aggtgtgtca
      361 gttgacaata gcgtgttgtc ctgctcttcc ggaacggtat cgaggaattt tggttttgca
      421 gttggcgcct gaattgcttc ccaaccagtc tgcttggctt gaatacgttc agaaatttgt
      481 gtggccgaat ggagctgcag ttcgaacagg cgcggcaagc tggcgtactg ccagagcggt
      541 acaaaactgg caataagtaa cgcagagccg accagtcgcc gcagccaggt gcgatagtgt
      601 gttgcataaa gcatcccagc tgccaggata cagcccaacc cgatccatgc gattacgtca
      661 taactcggcc aggcgagagt aaacgcaggg atgccttcaa ccacaagata aagtaacgcc
      721 gccaggatcg ccatctggct gaacagcaag atgggctgga cttgctctcg gaataccatc
      781 caacggctga ggcgatccca ttgtttgccg gcgcgctggg ccaggcgttc cagagacagg
      841 tcaaatgcag gactggcttg ccaacctgtc aaccttaacg tgaacaccag ctcgttcagg
      901 atcggatgca aaccgctgag tgcctggcac gattgatcaa ctggtaaacc actgcgccgg
      961 cgctggatag attcacggca agctgtctgg atttccccag gtggtagtcc gtccacaacc
     1021 ttgatgagtc gttcaaagaa atcctgcccg gcttcccgct ggccacgcaa accagcaatc
     1081 agcgcaagaa ctgcgagtaa cgaaatatcc ttttttgtca ggagcagttc aatgcataat
     1141 attgccaggg cgggcaggat caacagcagc cactcgcgca cgaaccaggc ttcaatccag
     1201 gaaattacaa cccatccaaa cagcaaccct gagactgata gcaaaagtac attccagggc
     1261 aacttgtccc tgagcggttt caaggatatc ctctgcccag ccaacaaacg ataaagccct
     1321 gctttccgag accaatggta aaagtccaaa cccaaccgcg ccagccaggc catcacaaag
     1381 gcgatggaaa agaaatagct gagcaagtat aacgagcgga tttccatcac cacactccag
     1441 ggatcaacct gaaacgcacc tgatgggcat acgccagata accggagata ataagctctt
     1501 cccggaggga gcgtaaaatc tggatggctg ccagagcgat gaacaatcca gctgccagga
     1561 aaggctgttg agatgtggaa accaacgcaa gacgcagcac cagttcgccc aggtacatag
     1621 ggtggcgcac aaagcggtag gggccgttag caaccaagcc ccggtcggct ggcgcaatgc
     1681 caaagcgcgc tcccagtgta agcagcgacc agagcaccag aacataaccg gatacaccaa
     1741 cgaaaagaag aaccagggga acctgttcag gatacggggc ggctataggc aggatagctg
     1801 ccagcacgcc cagccacagt ccgcgccggt cgtattctcg agcgggttta cggcgcgcat
     1861 acagtccagc caggatggca ttgtgaagcg ctgccagcca tgccagaatt gaggggtgct
     1921 cccaggctgc taatgctgag atcaccgccg caaaggtgaa gaaagccaga cccaggcggt
     1981 ctttcatccg aaatcaatct tccttgtcag tgtggttcga atggctcgat gaaccaccga
     2041 agtgaaacaa tgcccagatc catccgaaaa caaaaccaaa tgcgaaggta gaaccagcaa
     2101 tggatagggc ttgtgccgtc gtaataccat aatcgctcat ctcaacacct ccaaggagaa
     2161 agcctcgaaa ggcactacaa agtggccagg ataggccaac cgatcagaac caggatgacg
     2221 gtaatgaagg gcaggaagta aaagatcacc attgggatga ccagctccgc gccgacacgc
     2281 tcggcgcgct gctctgcacc agcagcgtat tcagcggcga tccgtccggc catctgcccc
     2341 atcaattcct gctgggcggt gcccaggcgg acgaattcca actgcaccgc cagcccaatc
     2401 aggtcgggca agtaggaggc ctgggcttct ttctgcaact gggtgaagat cacccgccct
     2461 tgtgacatct gcagcacatg gcgcatccat ttggctgcca ggctctcagt ctgagaagtg
     2521 cggcgcaagg cttcttcgag cgagacgccg gctgccatct gggcagcgac cagctggatg
     2581 aattccggga gctgcgattg gaagcgccgc cttactcgac gggcaacgcc gcctaacatc
     2641 acaacgggga cctgccaacc aagcaatgac gccagcagtg aagggatcat attgtcatag
     2701 actaacaagc caaagacaaa agccccaaga catagcgcga tgcgcaggga ggtgaactgc
     2761 acttcgtccc agccttccca cttcccaccc agttgggcaa agtacaaatc ggctttggcg
     2821 cggcgcaaga ttgacgccgg cgtccaacgg gcagtggagc gatccagctt cgagagccat
     2881 ttccgccaac caggcagctc aggtgcggca ggttggtcgc tttctcgtaa gaagcgagct
     2941 gctttgcctg ccggcaggct ggggacaagc tccacccata aggtgagcaa taatgcagcc
     3001 gtagcgatac caatcaagta agttatagtc ataccgcctc cgcaacaatc gaccggatca
     3061 acaggtaccc gatcaccatc aagccaattc ccatggcgat aacagcctgc accggaaaag
     3121 ccagcagcga gccgcggatc aatggatcgc gagtcaggaa gataaaaaca atcaacaaaa
     3181 ccaccgggat gatgcgggct gatagcagag catccccagc cttggccacc gcccggttgc
     3241 gcgagaccag gatctgctga acacgctggg agatctgcat cagcacttct gcgtattttc
     3301 cgccgccagc ttcgctgtat ccatcaagca ggtaagccag gttggaaagt gaagttgacg
     3361 gcgagcggtc cgccagactg cgcaaagcct gctggcgctc tttgctgcgc aattccgcag
     3421 cagtaagcat caattctggg gtgagagggt tagggctttc catatctagc gattctcctg
     3481 tcttctgcag cacctcaggc acggaaccac ccgccagcaa accagccgtg atacgtccta
     3541 ctgctactgg caacaagcgg tcaatctccc gcccacggct cttcacccga tcgtccaacc
     3601 aggcataggg aatgtaggct gaaatgatcg ccaggatcaa agcgggcagt ccaggcagca
     3661 tcggccaggt gatgacccct accgcaatac cccccgcaat cgtgagcagc ctgaaggtga
     3721 atggctgcaa gcgcaaccca gcagcgagca gcttgtattc cagcgtttcg ggtttcaact
     3781 cgctgagtgt tctagccgta tccagcgagt acatctcagc caggcgttgg ctggcttcac
     3841 cgtgccggat gcgcgctggt gagaataaaa cgaataccag taagcccacc aaaatcccaa
     3901 tcaaggtgat cataggcgca cctcctgaaa gctggtctca gccaggatgc gtgggcgcag
     3961 ctcgcccact ttctcccaac gcggctggct gcccagcgaa cgttcatcaa atcgatagat
     4021 tagctcaaag tggatatcgc cgttgctcaa ttccttagcc aggttggcga tcacggtcac
     4081 tcgccggacc tcgtcgcgga tgccgatttg aaccaagagg tctaccgctt cggcgatgag
     4141 ctgcacggca tccgtggcgc ccactccggc atctgctcca agtactgtcg tcaggcgacg
     4201 caccgcctct cggggattat cggcgtggaa ggtgcaggct ccggagtgcc cggtcatcat
     4261 cgcccggaac aggctcaacc cggcgtgccc atcgcgcacc tcgcccagaa tcagataatc
     4321 aggagccatg cgcagggcat catcgacagc gttggccagg gtataaggct ggatttccgt
     4381 gccaagggca gctgggcggg cttcgatggt ctggacagta gagcgctcga tccaaatctc
     4441 ctcaggatct tcgatcttta caattcgcca cgcagaaggc aggtagttgc ataacgctga
     4501 gagcagcgtg gttttaccgg tgcgcgtacc acctgtgatc aggatacggt aaccctgctc
     4561 catggcaatc tgtaaaatcg ccatcatctc cggactcatc atctcccgtt ccaatatcca
     4621 ctcaggtaga acaggttttt gctcaaagag ccggatattg atgctcggat tacgccctgg
     4681 aggagcgatc accgggtgca gcgctttgat gcggcctcca cccgggttat gcaacgttgc
     4741 cggcaacttg gcattgactg tgggattagc ctcgtttaga ctcttgtttt gcggacccag
     4801 gatgcgatcc aggacacgga taatctcgtg tggatcaggc tttacatcct ggagcgtttc
     4861 ccaacgcacg ctgttcttct tcatcacctg caccaggccg ctggaataga cgcaaatctc
     4921 tgaaagatcc gtgcgcgctg ggggaagcaa caaatccaaa aagcccagcc ccatcaaccg
     4981 tcgccccaac tcggctgcca aagcaccatc cgtctggtaa ccagaatagg cattactgcg
     5041 cctgaatgcc tgcccgacta gggtgttgat gcgttcctgg attcgctggc gctctgtatc
     5101 ggtgggcgaa cgcaggacat ttaccggaaa ttcgcggttg atctgatcga taacttcgtt
     5161 ggcgaccgtg tctttcaggc cttcatcaac caggcgttca gttccccgag ctacatcgta
     5221 caggctggat aatcccttgc cattactgtt gccaaacatc gcatctcctg acaaataaac
     5281 tgtctctcat gtaaaacgga agcgtgggag gcgcagccgg ctctttgtgc tgtgtccatc
     5341 cctgccattc accccagcca tcaacccacc ggggaacaga aaatccgcca ggctgcgcac
     5401 gccttttgcc aggtcatcaa cccgagtgac tgcgggtatc tgctcatctt gcgcctggct
     5461 gatcgccggg ttgtaaggga tggctgctgc aaccggtgga gcccatccat aactccccac
     5521 cagctcgtcg tgaaatccac gcagcgagat cggagagcga tctgtgacct ggttgagcac
     5581 caggtagatg gcttcacgcg ggatgcggtg ctcccccact aggcgttcca acaataggat
     5641 cagggtgtgg cgtgcagccg ccatgtctgc cagggtgcag cgcgagacga tcactgctgt
     5701 gttcgccgca gcgatgccct gcaggctcca gggctgctca ccggccggca gatccagcag
     5761 cactgcggca taattgcgcg tccaggaggc cattaccagc gaatagatgc tgtccggctc
     5821 agctttatga gcggtcgagt attccgctgc cttctggtaa tccaccgagt tttccggtga
     5881 catcaacaca tctaaacctt cccgcgtctg gatcgatgca gcaaatccat cgcctggacg
     5941 ggcaaaatat tccatagaat tgggaacgta gcgcattttc aagtggggca caacggacgg
     6001 cggcaggtca aatgacatca atagggaatg cacattcata cgcacgttta gctcgtaggc
     6061 caggctttcc gccagggtag aacgccccgt accaccagtc gccgagatga aggcaaccac
     6121 acgtgtaccg acgattgcgc tagaactgcg agaaagataa gcctgctgca gaggcgcagt
     6181 ggcagccgcc cgggcgcgct cggtcattac tgctgcataa ccagattgag caatctcacc
     6241 ccaattcacc ggagcgatgt acacgccgcg cacaaccgca gctctttcaa aaattccacg
     6301 cacatctcgg attgatggcg gtagcaccag aatagccacc ccatgccaga cctggatctc
     6361 agccaatacc tgcaccagcg cttctggacc aggggcgatt tccacctgta ccacgacaag
     6421 atcgggacgc atctgagcca ggctttgctc aaaagtcgtc cattgggtgg cgtgcgcact
     6481 gatcacgaac cggctgtcgg ataggaacgg cggctgcatc tggtagaaaa ccgtttcgtg
     6541 ccctggccca gccagcatca cagatacttt ttgatcggcg attggcgcgt tactcatgga
     6601 ttacttgcct ccgcgatctt cattcatggc gtcatataga tccgccagat tgagtgtcag
     6661 gatatccggc gcttcaaagc cgccagcggg ttccaggctc agatacaggc tgccatactg
     6721 gctcagcgca acgatcaagg tggtcggatt gacttgcaat ccagggacga gttcgatggg
     6781 agtggcaggt atatccagaa ccactacact cccttcctgg gcggctgaaa ccgtgcgggc
     6841 gctggcgaac aactgctctt cggttgatcc tgacggtagc tcttcatagc ggaaggactg
     6901 cggcaccatc agcactttca agccgctgat cgcaatacgc gccaggggag ccatcggcgg
     6961 tgcaggtgta ggggtgggag tgggcgtcgg agtaggcagg ccggttagag cagcagcggt
     7021 tggctcaggc aagaagctgg cattaattgg gatgggtgcc gaggagacgc tctttagaac
//
LOCUS       DATMXK010000532         3888 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_128909, whole genome shotgun sequence.
ACCESSION   DATMXK010000532 DATMXK010000000
VERSION     DATMXK010000532.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3888)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3888)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3888
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_128909"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1404
                     /locus_tag="VN363_09665"
     CDS             <1..1404
                     /locus_tag="VN363_09665"
                     /inference="COORDINATES: protein motif:HMM:NF024703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003676 - nucleic acid binding [Evidence
                     IEA];
                     GO_function: GO:0004386 - helicase activity [Evidence
                     IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016818 - hydrolase activity, acting on
                     acid anhydrides, in phosphorus-containing anhydrides
                     [Evidence IEA];
                     GO_process: GO:0006139 - nucleobase-containing compound
                     metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helicase C-terminal domain-containing protein"
                     /protein_id="HWQ84824.1"
                     /translation="EGHHLEDATTNALSFRVTLADIERLLRELGGPRGGLLGWIVNAT
                     QNLIPPSEQAVLSQLVQQATDRAFSFETLTRQFFQTLDRFLGEMREGRPVGAYSHQER
                     ILPATRTQPAWGDVELAWDEVEHVLGPLVEQVGHLAQGLAELSDELSEADGELYGSLT
                     NLYRRLTEMHSQMNGLVFTPEADRIYWVEAQPNGMRQALNVAPLHIGPLMQRYLWHEK
                     ASVILTSATLTAAGEFDYLRGRLFAEDADELALGSPFDYETAALVYVVNDIPEPSDRQ
                     GNQRAVENAIIRLCKATGGKALVLFTSYDQLRRTSRAVAPVLARDDIVVFEQGEGASS
                     HALLESFRSSERAVLLGTRAFWEGVDVPGDALSILVIVKLPFDVPSDPIVAARSETFE
                     DPFSQYSLPEAILRFRQGFGRLIRTQYDRGVVAILDQRILTKRYGRSFIDSLPPCTVH
                     SGPLSELPKSAVQWLNL"
     gene            1522..2373
                     /locus_tag="VN363_09670"
     CDS             1522..2373
                     /locus_tag="VN363_09670"
                     /inference="COORDINATES: protein motif:HMM:NF015251.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="lysophospholipid acyltransferase family protein"
                     /protein_id="HWQ84825.1"
                     /translation="MSRKLPEGAGYRFASSLGDWMGRRRSLEMVRAVRANQWVASSCQ
                     ADSDSLDRAARETYQHTARCLYDLYHNLQDIEKLYTRVDFSPRFIQFLEDRQKPSQGL
                     VLGGIHLSNYDLAIHAVGRRGLKAFALGVANPGSGYQWQNKLRAESGIQIIPSSANAL
                     RHAIHQLEANGTVITGLDRPLPDSKYQPSFFGRPAALPVMHIMLALKANVPIKVPYAL
                     MNHEGRYHMDISEPIEMEPYPDRHEEILRNAERVLSVAEGYIRRAPQQWAMFYPVWPE
                     ALPQIPV"
     gene            2405..3103
                     /locus_tag="VN363_09675"
     CDS             2405..3103
                     /locus_tag="VN363_09675"
                     /inference="COORDINATES: protein motif:HMM:NF013250.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0016780 - phosphotransferase activity, for
                     other substituted phosphate groups [Evidence IEA];
                     GO_process: GO:0008654 - phospholipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CDP-alcohol phosphatidyltransferase family
                     protein"
                     /protein_id="HWQ84826.1"
                     /translation="MDQNTQDPSMQDIKSHQRINDILLGPLERPALQFFARNMPAWVT
                     PDKLTTLGVLGSVLIFASYILTSRDPAFLWLASLGFVINWFGDSLDGTLARYRNIQRP
                     IYGFYVDHSLDAASESLIFIGLGLSPYVRLDLALLALVGYLLMSILVYIRTCVDGVFK
                     ISYGKLGPTEVRLIAIIANTVLFFSSNPQWITSVGTFGVYDLIVSVIIVLLFSFFITT
                     TFQDIRRLQHVDGK"
     gene            complement(3121..>3888)
                     /locus_tag="VN363_09680"
     CDS             complement(3121..>3888)
                     /locus_tag="VN363_09680"
                     /inference="COORDINATES: protein motif:HMM:NF012364.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000166 - nucleotide binding [Evidence
                     IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="DNA polymerase domain-containing protein"
                     /protein_id="HWQ84827.1"
                     /translation="VFKDGERQAADFQPLLNEILERTGLPIALDGIYRWVAFLPSRVD
                     RRVPVPNRYFGLFQDGSFKLRGIEVRRHDTPVFIKDVQMEMLELMGKTSYPVNSLPAQ
                     VTLLRRRLSELRSASVPLERLLVTQTLSRTLDEYRSPSPVALAVGQLVACGKTMRPGQ
                     RVRFLFTRGKPGVQAWDAPATGPVIPDLGRYVTLLVRAASSIVGPVGVDEQTLRWWLF
                     SNAGYGSRPGVLPPAPVTRQSTLSLPMWMALPSQVGD"
BASE COUNT          825 a         1066 c         1087 g          910 t
ORIGIN      
        1 gaaggccacc atctggaaga tgcgacaacc aacgccttga gcttccgggt gacgctggca
       61 gatatcgaac gcctgctgcg tgagctgggt ggacccaggg gcggcttact gggctggatt
      121 gttaacgcaa ctcagaactt aattccacct tctgaacagg cagtattgag ccagcttgtc
      181 caacaagcta cagaccgtgc ctttagcttt gagactctca cccgccagtt cttccagacc
      241 cttgatcgct ttctgggcga gatgcgcgag ggccggccgg taggagctta ttcccaccag
      301 gagcggatct tgccggcgac gcggacccaa cctgcctggg gagatgtgga gcttgcctgg
      361 gatgaggtgg agcacgtcct tggacctctg gtagagcaag tcggccatct ggcgcagggg
      421 ctggctgagc tctcagacga attatcagaa gcggatggtg aattgtacgg cagcctgact
      481 aacctctacc gccggctgac ggaaatgcat tcgcaaatga acggactggt tttcactccg
      541 gaagcagatc gcatttattg ggtggaagcc cagccaaatg ggatgcgcca ggctttgaat
      601 gtcgcaccac tccatatcgg ccccctaatg cagcgctatc tgtggcatga gaaagcatcg
      661 gtcatcctga catcagccac attgactgcg gcgggtgaat ttgattacct gcgcgggagg
      721 cttttcgctg aagatgcgga cgagctggcg cttggctctc cattcgatta tgagactgcc
      781 gccctcgttt atgtagttaa tgatattcca gagccttccg accgccaggg aaaccaacgt
      841 gctgtggaga atgctatcat ccggttgtgc aaggctaccg gtggaaaagc gctggtcttg
      901 ttcacttcgt acgaccagct tcgccgcacc tcacgagcgg ttgcaccagt attggcgcgg
      961 gatgatatcg ttgtttttga gcaaggtgaa ggcgcatcct ctcacgcttt gcttgaatca
     1021 tttcgcagca gcgagcgagc agttttattg ggcacgaggg ccttctggga aggtgtggac
     1081 gtaccgggtg acgccttgtc gatccttgtg attgtcaaat taccattcga tgtgccatcc
     1141 gatccgatcg tggcggcgcg ttcagaaacc ttcgaagatc ccttttcaca atactctttg
     1201 ccagaagcga tcttgcgttt tcgccaggga tttgggcgtc tgattcgcac tcaatatgat
     1261 cggggcgtcg ttgctattct tgatcaaagg atattgacta aacgctatgg gcggagcttt
     1321 atcgattcgc tcccaccctg cacagtgcat tctggacctc tgtctgagct tcccaaatca
     1381 gcagtccagt ggttaaattt ataggaagat caggctcagc gaatgctggg catgagcccc
     1441 aaaaaactac cacaggcgga catggcaaca ggatttcagg atctgatcaa tagtaaatat
     1501 ggggtgggac tggtgctctg ggtgagtcgt aaattgcctg agggagccgg ttaccgcttc
     1561 gcgtcatccc tgggagactg gatgggtcgc cgtcgcagcc tggagatggt tcgagctgtg
     1621 cgggctaacc aatgggtggc gagcagctgc caggctgact cggacagcct ggatcgtgca
     1681 gccagggaaa cctaccagca tactgcccgc tgcctctacg atctgtatca taatttacag
     1741 gatattgaaa aactctatac ccgggtggac ttttcacccc gtttcatcca atttttagaa
     1801 gatcgccaaa agcccagcca gggtttagtt ctcggtggca ttcacctgag taattatgat
     1861 ctggcaatcc atgcggttgg caggcgtggt ttaaaggctt tcgccctcgg agtagccaat
     1921 cctggttcag gttaccagtg gcagaacaag ctgcgggccg agtcgggcat ccagatcatc
     1981 ccctcttcgg ctaacgcttt acgccacgcg atccaccagc tggaggcgaa tggaacggtc
     2041 atcaccggct tggaccgtcc attgccagat tcaaagtacc agcctagctt ttttggccgc
     2101 ccggcagcac tgcctgtcat gcatattatg ctggccctta aggcgaatgt ccccatcaaa
     2161 gttccttacg ccctgatgaa tcatgaaggg cgttatcata tggatatctc agagccaatc
     2221 gagatggagc cgtatccgga ccgccatgag gagatactgc gaaatgctga gcgggtgctg
     2281 agcgttgccg aaggatacat ccgccgtgca ccgcagcagt gggcgatgtt ttatccggtt
     2341 tggccagaag cactgccaca aataccggtc taaaactatc acaaaaaggg aggttttgat
     2401 atcggtggac cagaacactc aagacccttc tatgcaagac atcaagagtc accagcggat
     2461 caatgatatc ctgctgggtc cgttggagcg tcctgctctg cagttcttcg ctcggaatat
     2521 gcctgcctgg gtcacacctg acaaactcac tacccttggc gtgttgggct cagtgctgat
     2581 cttcgccagt tacatcctca ccagccggga tcctgccttt ctctggctgg ccagtctggg
     2641 ctttgtgatc aactggttcg gcgacagcct ggatggcaca ctggcacgct atcgcaacat
     2701 ccagcgcccg atctatggct tttatgtcga tcattcactc gatgcagcga gcgagtccct
     2761 gatcttcatt ggtctgggtc tctctcctta tgtgcgcctg gacctggccc tgctggctct
     2821 ggtgggctat ttattaatgt caatcctggt gtatatccgg acctgcgtgg acggtgtttt
     2881 caagatttca tatggcaagc tgggtcccac cgaggtgcgc ctgatcgcga ttattgccaa
     2941 tacagtgctt ttcttttcca gcaatccgca gtggattact tctgtgggaa catttggagt
     3001 ttatgacctg atcgtgagtg taatcatcgt tctgttattc agttttttta tcacaacgac
     3061 ctttcaggat atccggcgtt tgcagcacgt ggacggaaaa taataccggg tttcttttta
     3121 ttaatcacca acctgggatg gcagagccat ccacatgggc aggctgaggg tactttgcct
     3181 ggttaccggg gccggcggca gaactccggg gcggctgcca taaccggcat tgctgaacag
     3241 ccaccagcgc aaagtttgct cgtccactcc cactggtccc acaattgagc tggccgcgcg
     3301 caccagcagg gtaacgtacc gtcccaggtc agggattacc ggcccggtcg cgggtgcgtc
     3361 ccaggcctgc actcctggct tgccacgcgt gaacaggaag cgcacgcgct gccccgggcg
     3421 catcgtcttt ccgcaggcta ccagctgccc aacggcgagt gctacgggtg aaggcgagcg
     3481 atactcgtcc agcgtacggc tcagggtctg agtcaccagc aagcgctcca gcggcacact
     3541 ggcgctgcgc agctcgctca agcggcggcg cagcaaggtc acctgagccg gcagactgtt
     3601 caccgggtag gacgttttac ccatcagctc cagcatttcc atttgtacat ctttaataaa
     3661 gaccggagta tcgtggcggc ggacttcgat accgcgcagc ttgaacgatc catcctggaa
     3721 cagcccaaaa tagcggttgg ggaccgggac gcgccggtcc acccgggaag gcagaaaagc
     3781 tacccagcgg tagatcccat ccagagcgat gggcagcccg gtgcgctcca ggatctcatt
     3841 cagcaaaggc tggaaatctg ctgcctggcg ctcgccgtcc ttaaatac
//
LOCUS       DATMXK010000533         6337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_40886, whole genome shotgun sequence.
ACCESSION   DATMXK010000533 DATMXK010000000
VERSION     DATMXK010000533.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 6337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 6337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..6337
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_40886"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1194)
                     /locus_tag="VN363_09685"
     CDS             complement(<1..1194)
                     /locus_tag="VN363_09685"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ84828.1"
                     /translation="MTQSGVTRRDFLKIGGAAAGALAATAGRLPAPVAQAAISAGLLD
                     PDGDGYIPTMCEMCVWRCGVRARVQNGRVVKLEGNPDHSHSKGKLCARGQSGLMTTYD
                     KDRVLHPLVRVGQRGEGKFRQATWEEALDIVAGQMLRIKQQYGPEAMIFSSTHNLSQT
                     QFENVLNGFGSPNYGTQRSLCFNAMIVAFSLTYGIEEPSRNYDNVRYIIMVGRNMAEA
                     ISTSETSAFVAALARGAKLVYLDPRFTKTAALADEWIPIKPGTDSAFLLAMIHEIVTY
                     ELGDCEFVKQYVIGCDDLPAAMAEYTPEWAESKCGVPADTIRRIAREFAAEKHNALIH
                     PGWRTSNFVNSFQTERAIATLNALSGNVLTPGGCLVAAAPEASGVSLGKPPQPPYPRV
                     SALRLD"
     gene            complement(1235..1462)
                     /locus_tag="VN363_09690"
     CDS             complement(1235..1462)
                     /locus_tag="VN363_09690"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84829.1"
                     /translation="MIPGFLGFLTGAAIFGLTYQQVFPVISKIANLGNVVIPDLWNLS
                     PFLTIVFFALLSVLLFYAIDRAGLIRKKSVE"
     gene            complement(1508..1792)
                     /locus_tag="VN363_09695"
     CDS             complement(1508..1792)
                     /locus_tag="VN363_09695"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HWQ84830.1"
                     /translation="MTTNMIIAVVVGIMFGFTLNKGGLTRYNKIVNVFRFTDLTVLKY
                     MLTALVVAMAGLYGLRSLGWITFPNVPATYIAGNLIGGLIFGIGMALSGY"
     gene            complement(1796..2329)
                     /locus_tag="VN363_09700"
     CDS             complement(1796..2329)
                     /locus_tag="VN363_09700"
                     /inference="COORDINATES: protein motif:HMM:NF016064.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="YeeE/YedE thiosulfate transporter family
                     protein"
                     /protein_id="HWQ84831.1"
                     /translation="MSSFVQQFRKELWSPYVAGIFLGITGILAVWLSDSLLGASGAFE
                     NVAGMIGKAIAPETFNNMYFNFIMPPELTWGVMLFIGTILGGFLGALSSGTFKFTVNG
                     DSQWKAIFGPQTWKRWVPAFLGAIVLEYGAGIAGGCTSGLAISGGMLLAPAAFIFIAG
                     MFASGIVTALIVYRKRY"
     gene            complement(2356..3309)
                     /locus_tag="VN363_09705"
     CDS             complement(2356..3309)
                     /locus_tag="VN363_09705"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84832.1"
                     /translation="MPNKKFSALLLMVAGVFLVATLLFLWPSGNASAQCGSQASSCKN
                     CHEVQGQDPVNSDGTGWHQSHAFGDFCAICHAGNAQSMNVDEAHTGMVSPLYDINASC
                     LSCHPTDVEALAQVYSVALGVEIGSGGPAAGSSGGSAPTGGSTASTGGTTGAAIAPAA
                     SAPIAASGSMVVDTSAVQEVVDYNQQYNETVLGQRTINWGNLILGVLTGLMVIGGGAF
                     VYTNERRLRGLPAFPKAAAAPAATEPVNPADYPQDVLAFLPQIARLNPFGRHALQRLL
                     EDPDQAAELLVGLSRLDPELVAKLRGLNRESRALLLALSAE"
     gene            complement(3390..3722)
                     /locus_tag="VN363_09710"
     CDS             complement(3390..3722)
                     /locus_tag="VN363_09710"
                     /inference="COORDINATES: protein motif:HMM:NF033788.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="metalloregulator ArsR/SmtB family transcription
                     factor"
                     /protein_id="HWQ84833.1"
                     /translation="MEATSLSQEVNQMHAEICSGLADPNRILLLYALAEGSHNVSELA
                     ELVGITQPSASRHLKLLRERGMVVANRQGPAIEYSLGDKRLIEALELLRAVLRDRINY
                     RASLIEGE"
     gene            complement(3930..5174)
                     /gene="nrfD"
                     /locus_tag="VN363_09715"
     CDS             complement(3930..5174)
                     /gene="nrfD"
                     /locus_tag="VN363_09715"
                     /inference="COORDINATES: protein motif:HMM:NF015851.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NrfD/PsrC family molybdoenzyme membrane anchor
                     subunit"
                     /protein_id="HWQ84834.1"
                     /translation="MAATATTPRPNKILTTPFILWTTSLLIVFAIGVVGMVIVLTQGL
                     HVTGLSDRVPWGLWITLDLSAIALGAGAFTFSAAVYLFRIKSLEPMARPAVFIGFLGY
                     TSAMLALAMDIGRPDRFYHPLIYWNVHSVLWEITWAVVLYSTVLIIEFLPLLLEGKFF
                     DRWPKLRRLGHTLHKATPVLAAIGMAISLIHQSSLGATYGVLSGRAIWFKPSLPVMFI
                     ISAVAGGMAMTLVAALVTGQITHKKLLPNRTVENVTRIIGVILLGYLYIKMWDWAATT
                     YYSVSPGTADALSRLRATTPYTNTFWGLEIVLGGLIPAIIIFVPRLRKNIWVLIFGLT
                     MIPMGVVVNRWNTTLSGLVVPPDWSPGVLGNVVAASYFPSGIEMMVAAGIVAYAWLGY
                     TLGVKFLPVYTEQPQEQKAPGD"
     gene            complement(5177..6016)
                     /locus_tag="VN363_09720"
     CDS             complement(5177..6016)
                     /locus_tag="VN363_09720"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="4Fe-4S dicluster domain-containing protein"
                     /protein_id="HWQ84835.1"
                     /translation="MSDNNSQDSEERKAPILTRRDLLKIVGATGGAVVLTEAVMKGPL
                     SAEAIQGIGSESHPGEGSSQQEWSMAIDLNKCIGCYYCVRACQAVNNVADPEMRWNIV
                     FPERTKEGADFFMNRPCQHCADAPCVRVCPVGATWIRPDGIVAMDYDRCIGCRYCEVA
                     CPYDVRRFNWDTPSDDNMFQPEWGTPEVPLRPRGVVEKCTFCVHRIDRAVELGLKPGV
                     DKAVTPACVAVCPVSARIFGDIKDPESPVSKFLAENDTFRLREDWGTNPRVHYVAPRI
                     QEA"
     gene            complement(6047..>6337)
                     /locus_tag="VN363_09725"
     CDS             complement(6047..>6337)
                     /locus_tag="VN363_09725"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84836.1"
                     /translation="VAGEATPAAPTGPVDDLLEDKFPSTSSVLTAEQRLQALEASMAS
                     QQLSTLFQGGVIGLVLGGTTAWFMASNTRRRPRDEGKPSENQNSENNGTLEE"
BASE COUNT         1404 a         1759 c         1896 g         1278 t
ORIGIN      
        1 atccaggcgc agggcggaga cccgcggata aggcggctga ggtggttttc ccagagatac
       61 cccggaggct tcgggggcag ctgcgaccag acagccgccc ggggtgagga cattcccgct
      121 gagcgcattt aaggtggcga tggcgcgttc agtctggaac gaattaacga aattcgaagt
      181 ccgccagccg ggatggatca gggcattgtg cttttcagct gcaaattcac gggcgatacg
      241 ccggatcgta tccgcaggca caccgcattt gctctcggcc cactcggggg tgtattctgc
      301 catggcggcc ggtaaatcat cacagccgat gacgtactgc ttgacaaact cgcagtcgcc
      361 cagctcgtag gtgacgatct cgtggatcat tgccagtaag aaagccgaat ccgtgcccgg
      421 cttaatcggg atccactcgt ccgccagggc cgcggtcttg gtgaaccgtg gatccaggta
      481 caccagcttc gcaccccggg ccagggcggc tacaaaagca gaggtttcgg aggttgagat
      541 cgcctctgcc atgttgcgcc cgaccatgat gatataccgg acattatcgt agttgcggga
      601 tggttcttcg atcccgtagg tcagggagaa ggcgacgatc atggcattga agcacaggct
      661 gcgctgagtg ccgtaattgg gggagccgaa gccgttcagc acgttctcga actgagtctg
      721 ggaaaggttg tgggtggagg agaagatcat cgcctcaggg ccatactgct gtttgatgcg
      781 cagcatctgc ccggcgacga tatccagggc ttcttcccag gttgcctggc ggaatttacc
      841 ttcaccgcgc tgcccgaccc gcaccagtgg atggagcacg cgatctttgt cgtaggtggt
      901 catcaagccg gattggccgc gggcgcacag cttgcctttg gaatgggagt ggtcggggtt
      961 gccttccagc ttgaccacgc gaccattctg gaccctggcg cgcacgccgc agcgccagac
     1021 acacatctcg cacatggtgg ggatataccc gtccccatcg ggatcgagca gaccggctga
     1081 gatggctgcc tgagcaaccg gagccggcag cctgccagcg gtagctgcca gagcaccggc
     1141 agccgcgccg ccaatcttga gaaaatctcg ccgggttaca ccggattgag tcataataat
     1201 ctcgtccttt gccaggtatt cactggatcc aactttactc gacgctcttt ttgcggatca
     1261 gtcctgcccg gtcaatcgca tagaacagca agactgaaag caaggcgaaa aagacgatgg
     1321 tcaggaatgg actgagattc cacaggtctg ggatcaccac attgcccaga ttggcgattt
     1381 tggagatgac tggaaagacc tgctggtagg tgaggccaaa gattgccgca ccggtgagaa
     1441 aacccagaaa tccagggatc aaataatcga gcttgccttc accgctcccg gcggcgcagg
     1501 tgcccggtca atacccgctg agggccatac cgatgccgaa aatcagcccc ccgatgagat
     1561 tcccggcgat atacgtggcg gggacattcg ggaaggtgat ccagcccagg ctgcgcaggc
     1621 cgtacaggcc ggccatggcg accacaaggg cggtcagcat atatttcaag acggtcagat
     1681 cggtgaagcg aaaaacattc acgattttgt tatagcgggt caggccaccc ttattgaggg
     1741 tgaagccaaa catgattccg acaacgacgg caataatcat attggtggtc atggcttaat
     1801 acctcttccg gtagacgatc agggcagtaa cgatgccgga ggcaaacata ccggcaatga
     1861 aaataaacgc ggccggagcg agaagcatgc cgccggagat tgccaggccg ctggtgcagc
     1921 cgccggcgat gcccgcccca tactccagca caatcgcgcc aaggaaagct ggaacccagc
     1981 gtttccaggt ctgcgggcca aagatggctt tccattggct gtcaccattt accgtaaact
     2041 tgaatgtgcc gctgctgagg gcgccgagga agccaccgag gatagtcccg atgaacaaca
     2101 tcactcccca ggtaagttcg ggcggcatga taaagttgaa atacatattg ttgaatgtct
     2161 cgggggcgat tgctttgccg atcatgccgg ctacgttctc gaatgccccg gaggcgccca
     2221 gcaggctgtc tgatagccag acagccaata taccggtgat ccccagaaag atcccggcga
     2281 cgtagggcga ccagagctct ttgcgaaatt gttggacaaa actgctcatt ttcccttcct
     2341 ccagttgttt ttttattatt cagccgagag cgccaggagc agggcgcgcg attcacggtt
     2401 caggccgcgc agcttggcga ccagctcagg atccaggcgg gaaagtccga ccagcagctc
     2461 ggctgcctga tccgggtctt ccagcaggcg ctgcagggca tgccgcccga agggattgag
     2521 ccgggcgatc tggggcagaa atgccaggac atcctgcggg taatcggcag ggttcaccgg
     2581 ctctgtcgca gccggtgcag cagctgcttt ggggaaagcc ggcaggccgc gcagcctgcg
     2641 ttcgttggtg tagacaaagg cgccaccgcc gatgaccatg agcccggtga gcacacccag
     2701 gatgagattg ccccagttaa ttgtgcgctg gcccaaaacg gtttcgttgt actgctggtt
     2761 gtaatccacc acctcctgca cagcagaggt atcgaccacc atgcttccag aagcagctat
     2821 cggtgcgctg gccgcaggtg ctatagcagc accggtagtg ccacctgtcg aggctgtgga
     2881 tccgccggtt ggcgcagagc caccgctgga gccagcagca ggtccgccgg aaccgatttc
     2941 aacgcccagg gctaccgagt acacctgagc cagggcttcc acatcggtgg ggtggcagga
     3001 gaggcaggaa gcattgatat cgtaaagcgg tgacaccatt ccggtatggg cttcatccac
     3061 gttcatgctt tgcgcattac cggcgtggca gatagcacag aagtcaccaa aggcatggct
     3121 ctggtgccag ccggtgccat cgctgtttac cggatcctgg ccctggactt cgtggcagtt
     3181 cttgcaagac gaggcctgag aaccgcactg ggcggaggcg ttaccggatg gccacaagaa
     3241 gagcagtgtt gcaaccagga aaacgccggc gaccatcagg aggagtgctg agaacttttt
     3301 atttggcata cttaccccac aacgcgaaaa atggtttcga gattattata cggggtgggt
     3361 ttttccccga agctgcagtt ttgcgaaatt tattcgcctt cgatcaggct ggctcgataa
     3421 ttgatccggt cacgcaggac ggcccgcagt aattccagcg cttcgataag ccgtttatca
     3481 cccaggctgt attcgatcgc cggaccctgg cggttggcaa ccaccatgcc acgctcgcgc
     3541 agcagcttga ggtggcggga ggcgctgggt tgggtgatcc ccaccagctc agccagctcc
     3601 gacacgttgt gagatccctc agccagagcg tacaggagca ggatccggtt cggatcggca
     3661 agacccgagc agatttcagc gtgcatctga ttgacttcct gtgagagtga ggttgcttcc
     3721 atttatgctc cttcgaaatg tacaagtatg cacataagcg catacatgca acattaagca
     3781 atattaatcc gccaaggcag tctgggatag tagcgtttgt cacaatcgca ggtgatattt
     3841 gtcacatagt cgcgtacatt tacagccaca aaaaaccacc ccgggaaatc tctcggggtg
     3901 gtttggggaa agttctggaa agtttatttt cagtcgccag gagccttttg ctcctgcggc
     3961 tgctcggtgt agaccggcaa gaatttaacc cccagggtat agccaagcca ggcataggct
     4021 acgatcccgg ctgctaccat catctcgata ccgctgggaa agtaactggc tgccacgaca
     4081 ttgcccaata cacctggtga ccaatccggc ggtacgacca gacccgatag ggtcgtattc
     4141 cagcggttga ctacaacacc catcgggatc atcgtcaggc cgaaaatcag gacccagata
     4201 ttcttgcgga gccggggcac gaaaatgatg attgccggga tgagcccgcc gagcacaatc
     4261 tccaggcccc agaaggtgtt ggtgtacggc gtggttgccc gcagccggga gagcgcatcg
     4321 gcagtgccgg gggagacgct gtaataggta gtcgccgccc agtcccacat ctttatgtac
     4381 aggtagccca gcaggatgac gccgataatg cgcgttacat tctccactgt ccggttgggt
     4441 aaaagcttct tgtgggtgat ctgcccggtc accagggctg caaccagggt cattgccatt
     4501 ccgccggcta cggcggagat gatgaacatc accggcagag atggtttgaa ccagatagcc
     4561 cggcctgaga gcacaccgta ggtggcaccc agggaggatt ggtgtatcag agagatcgcc
     4621 ataccgattg ctgctaacac aggtgtggct ttgtgcagtg tatggcctag cctgcgcaat
     4681 tttggccagc gatcaaagaa tttgccttcg aggagaagag gcaagaattc aatgatcagc
     4741 acagttgagt aaaggacgac tgcccaggta atctcccaca ataccgagtg cacattccag
     4801 tagatcaggg ggtgatagaa gcgatcgggg cgaccgatat ccatcgccag ggcaagcatg
     4861 gctgaggtat atcccagaaa gccgatgaaa accgccgggc gtgccatggg ctccagggat
     4921 ttgatccgga agaggtagac tgcggctgaa aaggtgaacg cgcctgcgcc gagggcaatg
     4981 gcagacaggt cgagagtgat ccacaatccc caggggacgc gatccgacag gccagtgaca
     5041 tgcaagccct gggtaagcac gatgaccatc cccaccacac caatggcgaa cactatcaat
     5101 aacgaggttg tccacaggat aaatggcgtg gtcaggattt tattcgggcg aggagtggtt
     5161 gctgtggctg ccatgctcag gcctcctgaa ttcgcggagc tacatagtgc acgcgcgggt
     5221 tagtgcccca atcctcacgc aggcggaaag tatcgttttc agcgaggaat ttcgacacgg
     5281 ggctttcggg gtctttgata tcgccaaaaa tccgggcgct gaccgggcag accgcgacac
     5341 aggccggggt cacggctttg tccacgccgg gtttgagacc cagctcaacc gcgcgatcga
     5401 tgcggtgaac gcagaaggtg cacttctcaa ccacacctcg ggggcgcagg ggtacttcgg
     5461 gtgtgcccca ctcgggctgg aacatattat catccgaagg tgtatcccag ttgaagcgcc
     5521 ggacatcgta ggggcaggcc acttcacagt agcggcagcc gatgcagcgg tcatagtcca
     5581 tcgccacaat gccatccggg cggatccagg ttgcgccgac cgggcagacg cgcacacatg
     5641 gggcgtctgc gcagtgctgg caggggcggt tcataaagaa atctgcgcct tctttggtcc
     5701 gctccgggaa gacaatattc cagcgcattt ctggatcggc tacgttgttg actgcctggc
     5761 aggcacgcac gcagtaatag cagccaatgc atttattgag atcaatcgcc atgctccatt
     5821 cttgctggct cgagccttcc ccagggtgcg actcgctgcc gatgccctgg atggcttcgg
     5881 ctgaaagcgg gcctttcatg acggcttcgg ttaaaactac cgcgccaccc gttgcgccaa
     5941 ctattttcag taagtcgcgc cgggtcagga tgggcgcctt gcgctcttca ctatcctgtg
     6001 agttattatc gctcattatc gagttacctc acccagttcg atctgcttat tcttcaaggg
     6061 taccattatt ttcactgttc tgattttcag aaggttttcc ctcatcccgc ggccggcggc
     6121 gagtgttgga tgccatgaac caggcggtcg ttccgcccag gaccaggccg attacgccgc
     6181 cctggaagag cgtggaaagc tgttggcttg ccatggaagc ttccagggcc tgcaggcgct
     6241 gctcggcagt caggacgctg ctggtggagg ggaatttatc ctcaagcaga tcatcaaccg
     6301 ggccggttgg agcagcgggg gttgcctcac cagctac
//
LOCUS       DATMXK010000534         3033 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_165428, whole genome shotgun sequence.
ACCESSION   DATMXK010000534 DATMXK010000000
VERSION     DATMXK010000534.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3033)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3033)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3033
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165428"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1214
                     /locus_tag="VN363_09730"
     CDS             <1..1214
                     /locus_tag="VN363_09730"
                     /inference="COORDINATES: protein motif:HMM:NF025925.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="ASKHA domain-containing protein"
                     /protein_id="HWQ84837.1"
                     /translation="LGSTTVAAFLTLLDTGEVQAGGASLNQQTVYGADVISRIAAALD
                     STVNADRLHRLALASINQAVDSLKMSDRIKKRIQRVTIVGNVAMHHLLAKLPVDSLAH
                     MPFQPYCKDAILDVGSLTGGIFAGDIQVSLPPLIGGFVGSDALACLAYFNFAQAQQPI
                     AAIDLGTNGEVMVTDGRRILTASTAAGPAFEGVNISCGTRAVDGAITAAQWKEGKLFL
                     QTIADQPPVGLTGSGLLSVVYELRKSGAIDASGRINPLIVAENERKGNGTEPRSIALT
                     EDGRLALTQWDIRELQKAKGAIRAAIEILMKNLGLQAGDIQRFILTGSFGGQVDIDGV
                     LALGMIPPVRREKVETIANGAGLGAAMFLSDEGMAAAVELAKLSEQIDLDLDPEFIQS
                     YIQAMVLAPND"
     gene            complement(1225..2334)
                     /locus_tag="VN363_09735"
     CDS             complement(1225..2334)
                     /locus_tag="VN363_09735"
                     /inference="COORDINATES: protein motif:HMM:NF015400.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="saccharopine dehydrogenase NADP-binding
                     domain-containing protein"
                     /protein_id="HWQ84838.1"
                     /translation="MRNPQWMIYGATGYTGRLIAQEAVRRGHRPILAARSALKLAPLA
                     EQLGLEHLVLDLSSNRVLAKAVTPYQAVLNAAGPYSSTGRLILDTCLLTQTHYLDING
                     EIPFLEYVLSQDQAARRKAITLLPGAGFDVVPTDCMAGYVTSQLTDPCYLDIAISGGS
                     QASAGTRRSSLEILANGGQVRRSGRLMSFPLGFDGRNVRFADRSRPVIPVPWGDLSTA
                     YYSTAVPNITTFMAFSPGSIRLLRGVGRAIPAILRWTRLRRWLQSRQGPSGKGPDPVS
                     NQDHRSQIYVRAINGSGQIAQAWLETLDGYRFTAFSAVSCLETVLQKQAAGAFTPVQL
                     LGMDWVLSVEHTRRLDTLPEHPSHPLAMSQPGRSS"
     gene            2633..>3033
                     /locus_tag="VN363_09740"
     CDS             2633..>3033
                     /locus_tag="VN363_09740"
                     /inference="COORDINATES: protein motif:HMM:NF023646.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="methylenetetrahydrofolate reductase C-terminal
                     domain-containing protein"
                     /protein_id="HWQ84839.1"
                     /translation="MPGFTPGRRWQPPYSPFKRESFRLKLLESVEKLVKGPLFGCRMC
                     GNCLLQETAFICPMECPKGIRNGPCGGSTEHCYVDETRPCMWYKIYERAFQMGREEKL
                     LEVMPPLDWDKVGGETWGDVVVQVKKLGTGNV"
BASE COUNT          660 a          788 c          945 g          640 t
ORIGIN      
        1 atctgggctc gacgaccgta gccgcttttt tgactctact ggataccggc gaagttcagg
       61 cgggaggggc cagcctgaac cagcagaccg tctacggggc ggatgtgatc tcgcgcattg
      121 cggcggctct ggattcgacg gtaaatgcag accgcttgca ccgcctggcg ctggcctcga
      181 tcaaccaggc ggtcgattct ctaaaaatgt ccgaccggat caaaaaacgc atccagcggg
      241 tgacgattgt aggcaatgtt gccatgcatc acttgctggc aaagctgccg gtggacagcc
      301 tggcgcacat gcccttccag ccatattgta aagacgccat ccttgatgtc ggcagcctga
      361 cgggtggaat attcgccggg gatatacagg ttagcctgcc cccgctgatc ggcgggtttg
      421 taggctcgga tgccctggca tgcctggcat acttcaattt tgcccaggct caacagccga
      481 tagccgcgat tgacctgggt acgaacgggg aagtgatggt gacggacggc aggcgcatcc
      541 tgacggcttc tacagccgcc ggaccggcct tcgaaggcgt aaatatttcc tgcgggacac
      601 gggccgtgga tggcgccatt actgcggctc agtggaagga gggaaaatta ttcctccaga
      661 cgattgcaga ccagccgccg gtaggattga ccggctctgg cttgctcagt gtggtgtacg
      721 agctgcgtaa aagtggggcg atcgatgcct ccgggcgaat taacccgctg atcgttgcag
      781 agaacgaacg gaagggaaat ggtaccgagc cgcgcagcat cgcactgacc gaggatggca
      841 ggctggcgct gacccaatgg gatatccgtg agctgcaaaa ggcgaagggg gctatccggg
      901 cagccatcga gatcctgatg aaaaatctgg gcttgcaggc cggggatatc cagcgtttta
      961 tcctgaccgg gtctttcggt gggcaggtag atatcgatgg cgtgctggcg ttggggatga
     1021 tcccacctgt gagacgtgaa aaggttgaga cgatcgccaa tggcgccggg ttgggggcgg
     1081 cgatgtttct cagcgatgaa gggatggctg ctgccgtgga gctggcaaaa ttgagtgagc
     1141 agatcgatct cgacctggat ccagagttta tccagagtta cattcaggcg atggtgcttg
     1201 ccccgaacga ttgattgtgt agattcagga ggatctacca ggctggctca tcgccagggg
     1261 gtgtgagggg tgttctggca gggtatccag gcggcgggtg tgttcgactg aaagcaccca
     1321 atccatgccc agcagttgaa ctggcgtgaa cgcgccggca gcctgttttt gcaagacggt
     1381 ttccaaacag ctcactgctg agaaggctgt gaatcgatat ccatccagag tttccagcca
     1441 ggcctgtgca atttgaccac taccgttgat cgcccggaca tagatctgcg atcggtgatc
     1501 ttgatttgag accgggtcag gtccttttcc ggagggaccc tggcggcttt gcagccaccg
     1561 ccggaggcgt gtccagcgga gtatagccgg gatcgcccgg ccaactccgc gcagcaggcg
     1621 gatggatccc ggtgaaaaag ccataaaggt tgtaatattg ggtaccgcgg tcgaatagta
     1681 ggcagtgctg agatctcccc agggcaccgg aatgaccggg cgggatcgat cggcaaaacg
     1741 aacattccgg ccatcaaatc ccagtgggaa actcatcaga cgcccactgc ggcgtacctg
     1801 cccaccattc gccaggattt ccagggacga gcgtcgtgta ccggcgctgg cctggctgcc
     1861 tccactgatg gcgatatcca gataacacgg atccgttaac tgggaggtga cataacctgc
     1921 catacaatcg gtcgggacaa catcaaaacc ggctcctggc agtagggtga ttgctttgcg
     1981 ccgggcggct tgatcctggg agaggacata ttcaaggaag gggatttcgc cgttgatatc
     2041 caggtagtgg gtctgagtga gcaggcaggt atccaggatc aggcgtccag ttgaggaata
     2101 agggccggct gcattaagga cagcctggta tggcgtcaca gcttttgcca ggacgcggtt
     2161 tgagcttaag tccagcacca ggtgttccag gcctaactgc tcagccaggg gagccaattt
     2221 aagagcagag cgggcagcga ggatgggacg gtgaccgcga cggacagctt cctgggcaat
     2281 taacctgccg gtatagccgg tagccccata gatcatccat tgagggttgc gcaaggctcc
     2341 tctcagcttc tggcgatgtc ccacaattat tcgaaggcga taattatacc ctgccgccaa
     2401 tcgtttctgg cacggttccg gcatactaca ggctggatat cagaatattc acctcccaca
     2461 ggcaatggcg ctctaccgca ggcgcggttt cccactatct cgatatctgg caccttattc
     2521 ctgacttttt ccccgggcgg acataatata tgtcacttgc cagccggggg tggttttgtt
     2581 atagtgtggg cgacatactc tgagtttcac tactgtagaa atgaggtcgt acttgcctgg
     2641 atttacacct ggtcgccgct ggcagccccc atacagccct ttcaaaagag aatcttttcg
     2701 cctcaagctg ctggagagcg ttgagaagct ggtcaaagga cccttatttg gctgccggat
     2761 gtgcggcaac tgcttgctgc aagagacagc cttcatctgc cccatggaat gcccaaaggg
     2821 catccgcaac gggccatgcg gcggatcgac cgagcactgc tatgtcgatg aaacccgccc
     2881 gtgtatgtgg tacaaaatct atgagcgagc cttccagatg gggcgcgaag aaaagctgct
     2941 cgaggtgatg ccgcccctgg attgggacaa ggtaggtggt gagacctggg gggatgtggt
     3001 tgtacaggtc aaaaaactgg gaacaggtaa tgt
//
LOCUS       DATMXK010000535         2450 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_117473, whole genome shotgun sequence.
ACCESSION   DATMXK010000535 DATMXK010000000
VERSION     DATMXK010000535.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2450)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2450)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2450
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_117473"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            92..1066
                     /locus_tag="VN363_09745"
     CDS             92..1066
                     /locus_tag="VN363_09745"
                     /inference="COORDINATES: protein motif:HMM:NF024091.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWQ84840.1"
                     /translation="MSEEIVTEAIEPNPAAPAPARGGVVARLQGWLLGNPVTLKELRG
                     RMRGRRAYILITAYTAVLALAMILVYFATANATSPNNLDSLRTVGKALFASVFILQLL
                     AVCFIAPALSAGAIATERERQTYELLRTTLLSAPRLVRGKFTSGLFFLLLLLLVGLPL
                     QSVAYLFGGVTLEELLVGILVLVVTAIAFLAIGLFFSSLARRTLVATVLAYGASLLLV
                     FGLPLLILVITLMQNNLFTGFSTSSGYQAAFLMVLMWIVICINPLAAAIGSEVVLMEN
                     DSLWSMTQTFTGVSLTLPSPWYVYSIVYILLSLLLLWLTVRRVSHPDR"
     gene            1072..>2450
                     /locus_tag="VN363_09750"
     CDS             1072..>2450
                     /locus_tag="VN363_09750"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84841.1"
                     /translation="MASTPLQELQVLFGECLARYRLARALRWSGAGLAVGAGVAFLLG
                     AALTWLRLVLPAELAWLVGILLLSGSGLGLLSGIAWPVPRLPAARRFDRQFGLQERLS
                     TALEQAEAPAASSLAPLQLADALVTARKVDLAQVLPLRLPWRSLLAAVLLCALTAGMA
                     WGLRSQFELAQQQRELEAAITSEAEALEELSTEIEQLDSLAPEAETAILESLQATIQD
                     LQASGTLEEAFTALAEGQAELQAQISPDSQATLEALAQAGQALSQSENPDLQTAGEAL
                     SQLDLAEAAAALENIDPSQLSASELEQLSEQLSAAAEALQSSQPAAAAALQNAADAAA
                     AAASAANAAQSGDPGQPGQGEQADQAAQAAAAQAAEAAAQAAADSLQQAVAELQSLEG
                     QLAAGQAAMQAANSLAQAQAALTQARAALGGAGAGTGLSAPGQPGAGQSGSGTSSDPA
                     AQNPGTGGGA"
BASE COUNT          376 a          830 c          784 g          460 t
ORIGIN      
        1 ccggggtgag cgtgttgaat ttctccgccg accagcgcga cctggaagaa gtcttcctgc
       61 gcgccacccg cggccaggtg acctgaagca aatgagcgag gaaattgtga ccgaagcgat
      121 cgaaccgaac cccgcagcgc cagccccggc cagaggcggc gtggtggcgc gcctgcaggg
      181 ctggctgctg ggcaatcctg taacattgaa agagctgcgc gggcggatgc gcgggcggcg
      241 ggcatacatc ctgatcaccg cctacaccgc ggttttagcc ctggcgatga tcctggtcta
      301 ttttgcgacc gccaacgcca ccagccccaa caacctggac agcctgcgca cggtcggcaa
      361 agccctgttt gcctccgtct ttatcctcca gctcctggca gtgtgtttta tcgccccggc
      421 gctgtctgcc ggcgccatcg ccaccgagcg cgagcgccag acctacgagc tgctgcgcac
      481 caccctgctt tcggcgcccc ggctggtgcg gggtaaattt acttccggtt tattcttcct
      541 gctcctgctc ttgctggttg ggctgcctct acagagcgtg gcttacctgt tcggtggggt
      601 gaccctggag gagctgctgg tggggatcct ggtgctggtg gtcaccgcga tcgcattttt
      661 ggcgatcggg ctgttctttt ccagcctcgc ccggcgcacc ctggtcgcca ccgtgctggc
      721 atacggcgcg tccttgctgc tggttttcgg cctgcccctg ttgatcctgg tgatcacatt
      781 gatgcagaac aatctgttca ctggattcag tacctccagc gggtatcagg cggccttcct
      841 gatggtcttg atgtggatcg tcatctgcat taatccgctg gcagctgcca tcggcagcga
      901 agtcgtcctg atggaaaacg acagcctgtg gagcatgact cagaccttca cgggcgtctc
      961 gctgaccctg ccttctccct ggtatgttta ttccattgtt tatatcctgc tcagcctgct
     1021 cctgctctgg ctgaccgtgc gccgcgtgag tcacccggac cggtaaatgt gatggcatcc
     1081 actccactgc aagagctgca ggttttattt ggggaatgtc tggcgcgcta ccgcctggcc
     1141 cgggctctgc gctggtcggg cgccggcctc gccgtgggag cgggggtggc attcctgctg
     1201 ggcgcagccc tcacctggct gcgcctggtc ctgcccgctg agctggcctg gctggtgggt
     1261 attttgctcc tgagtggctc tggcctgggc ttactgtcgg gtatcgcctg gcctgtgccg
     1321 cgcctgccgg ctgcccggcg cttcgatcgc cagtttggcc tgcaggagcg cctgagcacc
     1381 gccctcgagc aggcggaagc tccggctgct tccagcctgg cgccgctcca gctcgccgat
     1441 gccctggtta ctgcccggaa agttgatctc gcccaggtgc tccccctgcg cctgccctgg
     1501 cgcagcctgt tggcagccgt tttgctgtgc gccctcaccg ccgggatggc ctggggactg
     1561 cgcagccagt tcgagttagc ccagcagcag cgcgagctgg aagccgccat caccagcgaa
     1621 gcggaagcgc tggaagagct gtccacagaa atcgaacaac tggactcgct ggctcccgaa
     1681 gcggaaacag ctatcctgga atccctgcag gcgaccatcc aggatttaca ggcctccggg
     1741 acgctggaag aagccttcac cgctctggca gaaggccagg ccgagctgca ggcccagatc
     1801 tcacccgatt cgcaggctac cctggaggcg ctggcccagg ccgggcaggc gctcagccag
     1861 tccgaaaacc cggacctgca aacagccggc gaggcgctct cgcaattaga cctggccgaa
     1921 gcagccgcag ctctggaaaa tatcgatccc tcccaattgt ctgcttctga gctggagcag
     1981 cttagcgagc agctttctgc cgccgctgag gcgctccagt ccagccagcc ggctgccgcg
     2041 gccgccctgc agaatgctgc cgatgccgcc gccgcggctg cgtcagctgc gaatgccgcc
     2101 caaagcggcg atcccggcca gcctggccag ggggagcagg ctgaccaggc ggctcaggcg
     2161 gccgctgcgc aggctgccga agccgccgcg caggccgcag cagactcact ccagcaggca
     2221 gtcgccgagc tccaatccct ggaaggccag ctcgccgctg ggcaggccgc catgcaggca
     2281 gcgaacagcc tggctcaggc ccaggcagcg ctcacccagg cgcgagcggc cctgggtggg
     2341 gcaggcgcgg gtacaggttt atcggccccc gggcagcccg gtgccgggca gtccggctcc
     2401 ggcacgagct ccgaccccgc ggctcagaat cccggcacgg gaggtggggc
//
LOCUS       DATMXK010000536         5286 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_85001, whole genome shotgun sequence.
ACCESSION   DATMXK010000536 DATMXK010000000
VERSION     DATMXK010000536.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5286)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5286)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5286
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85001"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..907)
                     /locus_tag="VN363_09755"
     CDS             complement(<1..907)
                     /locus_tag="VN363_09755"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84842.1"
                     /translation="MGISQIKQLKGLSLPPTNHRWLRLSALGLVFACLFSFLPAILSN
                     ARIMDDLAAQRVAMWDDTDYQMIAVNVLHRYGFTVTPHMPYEEYKTMRDESKLVPDYQ
                     FYRAPGFPILLTGLYAITGVSTLAARIMVGVILWLTAVLLVWIGDRLAGWVGSLAGGL
                     AGYFLFKVSLLVNGTEGYFKGRTLSEPLTAFLITLFALLCIFYLQKRRRLYLYLACLT
                     LTSVALTRANFLAALPIFLVLVFIETRQWKPVLISAVLLFLPVLAWSGYASYTRQSPV
                     LLTTQGSIDFPRFNNQDVITGFGPEK"
     gene            complement(925..1995)
                     /locus_tag="VN363_09760"
     CDS             complement(925..1995)
                     /locus_tag="VN363_09760"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025072.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ84843.1"
                     /translation="MSNSSPPQRPKIHVIYEYGVDYRPHASSYVRLLRPLSHPRTAEW
                     AQFSYGREIIPPGTDLVIVDRLWRPDITLQLAEKLLDEIRYAGAKFICCLDDNLLDLP
                     LERLDWPRPEHLAILRYWLQQADGVMVSTPYLSERLQPFNSRIFTIANALDERLLVRR
                     PIRPAVNADRRLVIGYMGTQTHDEDLRLILPALRAINDRRPGQVVLEIIGVTSNPETL
                     ESLHGLPVRWISPDARESEYPLFMLWFTSQVNWDIALAPLRDTIFTRAKSDIKYLDYA
                     SIGAPAIFSDVAAYSATIQNGQNGVLVANTSSAWESAIESLITSTDLRHNLAHQANSE
                     LYNTRIINCCAVNWESALKSFL"
     gene            complement(1988..4861)
                     /locus_tag="VN363_09765"
     CDS             complement(1988..4861)
                     /locus_tag="VN363_09765"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF025025.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase"
                     /protein_id="HWQ84844.1"
                     /translation="MTGSTLEPVEPGATIPIQPGLSGLHSELEQILSDRFASQKIAIF
                     GQENQALIASLQQNGLHIQSSENARTIGEKLDLLVVLSSQTNPDDLSPILVENAGSVL
                     LVQESSLQPGFSTSGVEAWLNWLAQHGFHPDLNYRPVIAGRQAIYLQRYESSSEAGLA
                     AQYEQLIWSLESETAARRALLLEMRDELSVNGFDAAQIPVRLKVAELRQENEVLKQAY
                     QEIYNSRSWKLMRRVQNVRQRIIPPGSQREHRIRSLYRGLVVMSKEGPLPGLKRSSRR
                     ISWRLRSRLQSKRFQSPEYIQSQSFEVPPIQDRPTPGPHTAQIDIIICVHNALEDVKR
                     CLQSVVTHSTLPYHLILVDDGSQPETRDYLAAFSLQYASLLLRNETARGYTFAANQGL
                     QAAQGDFSVLLNSDTIVTPGWLDQMTACALSDDKIGVVGPLSNTASWQSIPNLSEDGD
                     WATNPLPHGMTVATMGTLVASRSARLYPEMKLLNGFCLFIRRAVLEQIGHFDEEKFGA
                     GYGEEDDFCLRARAAGWKLALADDTYIYHAQSKSYSSERRKQLYEKAGQNLREKHGER
                     IIQAGVEFNQFNPVIQGIRARSQIWNSRQELITAGERTYRGKRVLFLLPIMFAGGGGN
                     VIISEALAMRRMGVEVQFLNLPEFSQAFRQSYPDLNIPVIYAALQDLIQIGSRFDAVV
                     ATINTSVAWLQDLQTAQPQIKLGYYVQGFEPLMYSEGSPEYQNALHSYTLFPGLIPFT
                     KTAWTRQQVQDHTGAICQIVGPSVEIDLFQPRPSIWRVEGDQTVKIAAMVRADSAYRA
                     PRLTMEVLQKASWRYSPAVEILLFGIDPEHPEFSKLPVEFAWRATGLLSPRQVARLMS
                     EIDIFVDFSQHQAMGLSALEAMACGAAVIVPQNGGAVEFVRDGQNGLVVDTTSMNACW
                     QALQRLIDDAALRRKIQDQALQDVCQYYPEQAASRILKALFNE"
     gene            complement(4869..>5286)
                     /locus_tag="VN363_09770"
     CDS             complement(4869..>5286)
                     /locus_tag="VN363_09770"
                     /inference="COORDINATES: protein motif:HMM:NF025877.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Wzt carbohydrate-binding domain-containing
                     protein"
                     /protein_id="HWQ84845.1"
                     /translation="SNICLYDSRGQEQTIFQTGEPLILQMDYLAHEEVRSPVFGVAIH
                     RQDGTHITGPNTSLAHFEIPTVKGKGRITFKVPFLPVLEGLYQFSVAAINHDDTEMFD
                     YHDRVYPFRVINRNGEIQEPYGMITVAGEWSHQKLD"
BASE COUNT         1258 a         1353 c         1468 g         1207 t
ORIGIN      
        1 gcttctcagg accaaaacca gtaataacat cctgattatt gaaccgcgga aagtcgatac
       61 ttccctgggt tgtgagcaga acgggagatt ggcgggtata ggatgcgtac cccgaccagg
      121 cgaggacagg caaaaaaagg agcacagccg agatcagcac cggcttccat tggcgtgttt
      181 caataaatac cagaacaaga aagatcggca gggctgccag aaaatttgcc cgcgtcagag
      241 cgacacttgt cagggtcagg caggccaggt acaggtataa acggcgtctt ttttgcaggt
      301 aaaagatgca cagcaaggcg aacagggtta tcaggaaggc agtcaggggt tctgaaagcg
      361 tccggccttt aaaataacct tccgtgccat ttaccagcaa gctgaccttg aataaaaaat
      421 acccggccag tccacctgcc aggctgccta cccaccctgc caggcggtca ccgatccaca
      481 ccagcaaaac agctgtcagc cagaggatga ccccgaccat tatgcgggca gccagagttg
      541 aaactcctgt tattgcatac agtccagtca ggagtatggg aaatcccgga gctctataaa
      601 actggtaatc tggaacgagc tttgactcat cccgcatggt tttatattct tcgtagggca
      661 tgtggggcgt tactgtaaaa ccatacctgt ggagcacatt aaccgcaatc atttgataat
      721 ctgtatcatc ccacatcgcc acacgctggg ctgcgaggtc gtccataatc ctggcattcg
      781 ataggatggc gggtagaaac gaaaataagc aggcgaaaac caaccccaat gcgctcaagc
      841 gcagccagcg gtgattcgtg ggcggtaagg acagtccttt gagttgtttg atttgggata
      901 tacccaaggt cgggatttta ccttttaaag aaaggattta agtgcggatt cccagttaac
      961 tgcacagcaa tttataatgc gcgtgttgta aagctccgaa ttggcctggt gggctagatt
     1021 atggcgtaaa tcggtactgg taattaacga ttcaatagcc gattcccatg cagaagaggt
     1081 gttagcgacc agtacaccat tctgaccatt ctggatggtc gccgaatagg ccgcaacgtc
     1141 gctgaaaata gcgggcgcgc cgatggatgc ataatccaaa tatttaatat cggatttggc
     1201 tcgcgtgaag atagtatccc gcagcggcgc cagggcaata tcccagttta cctggctggt
     1261 gaaccagagc ataaataagg gatattcgct ctcgcgcgca tccgggctga tccagcgcac
     1321 cggcaggcca tgcagggact ccagcgtttc ggggtttgag gtgaccccga tgatttccaa
     1381 caccacctgc ccgggtcgcc ggtcattaat cgcacggaga gcgggcaaga tcaagcgcag
     1441 gtcctcatcg tgggtctggg tacccatata tccaataacc aggcgccggt ctgcatttac
     1501 agctggcctg attgggcggc gcaccagtaa gcgttcatcc agcgcattcg cgattgtaaa
     1561 tatcctcgaa ttgaaaggtt gtaatcgctc ggaaaggtaa ggtgtgctga ccataacacc
     1621 atcggcttgc tgcagccagt agcgcagaat ggccaggtgc tcggggcgcg gccagtccaa
     1681 tctttccagt ggcaggtcga gcaggttgtc atccaggcag cagataaatt ttgcaccggc
     1741 atagcgaatc tcgtccagca gcttttctgc cagctgcagg gtaatatcgg gtcgccacaa
     1801 gcggtcgacg atgaccagat ctgttccggg agggataatc tctcgcccat agctgaactg
     1861 cgcccattca gccgtgcggg ggtggctgag tgggcgcagg agacggacat aagaactggc
     1921 atgtggacgg tagtctacgc catattcata gataacatgg attttaggtc gttggggagg
     1981 ggaagaatta ctcattaaac agagctttga ggatcctgct ggcagcttgt tccgggtagt
     2041 actggcagac atcctgaaga gcctgatcct ggattttccg gcggagggcg gcatcgtcga
     2101 tcaggcgctg cagggcctgc cagcatgcat tcatgctggt cgtatcgaca accagaccat
     2161 tctgaccgtc acggacaaat tcaactgcgc ctccgttctg gggcacgatc actgccgctc
     2221 cgcaggccat cgcttccagc gcagaaaggc ccatcgcttg atgttgcgaa aaatcgacaa
     2281 aaatatcgat ctcgctcatc agccgggcaa cctggcgtgg actgagcaga ccggtagccc
     2341 gccaggcaaa ctcaaccggt aatttagaaa attcaggatg ttcgggatca atcccaaata
     2401 ataagatctc aaccgccggg ctgtaacgcc agctggcttt ctgcaggacc tccatggtca
     2461 ggcggggtgc gcggtaagct gaatcagcgc gcaccatggc agcaattttg acagtctggt
     2521 ctccttctac tctccatatc gatgggcgag gctggaagag atcaatttcc acactgggac
     2581 caacgatctg gcaaattgct cccgtgtgat cctgaacctg ctggcgcgtc caggcagttt
     2641 tggtgaatgg gatcaagcca gggaatagcg tataagaatg cagggcattt tgatactctg
     2701 ggctgccttc agagtacatg agcggctcaa agccctgaac atagtagcca agttttatct
     2761 gtggttgggc tgtttgcaga tcttgaagcc aggcaacact ggtattgatc gtggcaacca
     2821 cagcatcaaa tctgctccca atctggatca gatcctgtag ggctgcgtag attacgggga
     2881 tattcagatc cggataactt tgccggaacg cctggctgaa ttctggcagg tttaagaatt
     2941 gtacttccac gcccattcgg cgcatggcaa gcgcctcaga gatgatgaca ttcccgccgc
     3001 cgcctgcaaa catgatgggc agcaggaaaa gaacacgttt accacgatat gtgcgttcgc
     3061 cagccgtgat cagctcctgg cgagaattcc agatctggct gcgggcgcga atcccttgaa
     3121 tgaccgggtt gaactggttg aattccaccc cggcctggat aatccgctca ccgtgttttt
     3181 cgcgcaggtt ctggccggct ttttcgtaca actgcttgcg ccgctcgcta gaataacttt
     3241 tcgattgggc atgataaata tacgtatcat ctgccagcgc cagcttccag ccggctgccc
     3301 tggcgcgcag gcagaaatca tcttcctcgc cgtaaccggc gccaaatttt tcctcatcga
     3361 agtgaccaat ttgttccagc acagcccggc gaatgaacag gcagaagcca ttgagtaatt
     3421 tcatctccgg gtacaagcgc gctgatctgg aagctaccag ggttcccatg gtggcgacgg
     3481 tcatgccatg cggcagtgga ttggtcgccc aatcaccatc ttctgagagg tttgggatgg
     3541 attgccagga ggcggtattt gaaagcgggc ctacgactcc aattttgtca tccgacaatg
     3601 cgcaggcagt catttgatcc agccagcccg gggtgacgat cgtatcgctg ttcaataata
     3661 cagaaaagtc gccctgggca gcctggagac cttgattggc ggcaaaggtg taaccccggg
     3721 cggtttcatt ccggagcaag aggctggcgt actggagaga aaacgctgcc aggtaatcgc
     3781 gggtttcagg ctggctgcca tcgtcaacca ggatcaaatg ataaggtaaa gtcgaatggg
     3841 tgaccacaga ctggaggcag cgttttacat cttcaagcgc attatgcaca cagatgatga
     3901 tatcgatctg agccgtatga ggtcccgggg ttggccggtc ctgtatcggc gggacttcga
     3961 aggattggct ctgaatatat tctggagatt gaaaacgctt actttggagg cgagagcgca
     4021 atctccagga gatccgccgg gaagatcgct tcaggcctgg taagggacct tcctttgaca
     4081 tgacaaccag gccccggtaa agtgatctta tccggtgttc acgctggctg ccaggcggga
     4141 ttatccgctg gcgcacattc tgcacccggc gcatcagctt ccagcttcta ctgttataaa
     4201 tttcctgata ggcttgtttg aggacttcgt tttcctggcg gagctcagcc accttcaagc
     4261 ggactggaat ttgagctgcg tcaaacccgt ttactgaaag ctcgtcgcgc atctcgagca
     4321 gcagtgcccg gcgagccgct gtctcacttt ccaggctcca gatcagctgc tcgtactgcg
     4381 cggccagccc ggcttctgag gatgattcat atctctgtaa atagatggcc tgccggcccg
     4441 cgattacagg acggtaattg agatcgggat ggaagccatg ctgcgccagc cagttgagcc
     4501 aggcttcaac tccggaggtt gaaaagcctg gttgcaggct ggactcctgg acaagtaaaa
     4561 cagagcctgc attttcgacc aggatgggtg acaggtcatc tggatttgtt tgcgagctga
     4621 ggacaaccag gagatcgagc ttttctccaa tcgttctggc gttttctgaa ctctgaatat
     4681 gcaagccatt ctgctgaagg cttgctatta gagcctggtt ctcctgcccg aagatagcaa
     4741 tcttttgaga ggcgaagcga tcgctgagaa tctgttccaa ttcagagtgc aacccagata
     4801 agccgggttg aattgggatt gtggctcccg gctcgacagg ctcgagggtg ctgccagtca
     4861 tagggagctc aatcaagttt ctggtgcgac cattcgccgg ctacggtgat cattccgtac
     4921 ggttcctgga tttctccgtt ccggttgatg acccgaaacg ggtaaacccg atcatggtag
     4981 tcaaacattt ccgtatcgtc gtgatttatg gctgccactg agaactggta aagcccttcg
     5041 aggaccggca ggaaaggtac cttaaaggta atccggcctt ttcctttcac agtgggaatc
     5101 tcgaagtggg ccagggatgt atttgggccg gtaatgtgag taccgtcctg gcggtgaatg
     5161 gctaccccaa acaccggcga ccggacttct tcatgagcca ggtaatccat ctgcaaaatt
     5221 aatggttcgc cggtctggaa gatggtttgc tcctgaccgc ggctgtcata caggcagata
     5281 ttcgac
//
LOCUS       DATMXK010000537         2597 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_74842, whole genome shotgun sequence.
ACCESSION   DATMXK010000537 DATMXK010000000
VERSION     DATMXK010000537.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2597)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2597)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2597
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_74842"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1783
                     /locus_tag="VN363_09775"
     CDS             <1..1783
                     /locus_tag="VN363_09775"
                     /inference="COORDINATES: protein motif:HMM:TIGR01511.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="cation-translocating P-type ATPase"
                     /protein_id="HWQ84846.1"
                     /translation="GVVVVGEWMGVFEWLNQRIPFAIGLLIVLAGGYPVFANVIRATL
                     KRQIISHTLMTIGVLAALAVGEWVTAAIVVIFMRVGDYVENFTTESARRAVKDLTALA
                     PQTARVERDGAEMNIPVEQVIPGETIIVRPGEKIPVDGSVLSGQATVDQAAITGESMP
                     VEVTVGGQVYAATLAKLGSLRIRAERTGADTTFGRVIKMVEEAEANRAEVQRFADKFS
                     AWYLPVVAGIAGLTFVFSRNPIATAAVLLVACSCSIALATPVAMLASIGASARRGLLI
                     KGGKYLELLARADVLLVDKTGTLTLGQPQITDVVLLNGSSRAEALGYAAAAERFSEHP
                     LAEAVRRLAGAENIQLDEVEEFRAIPGEGVRALVRGQAVAVGNVRLLPAFASLETAHK
                     LESQGKTLLYLQVDGAPAAIFAAADTLRGEVPAALAAVRELGIRQVELLTGDNERTAA
                     ALGEKLGVSYRAGLLPQDKIAIVKEYQARGHIVVMVGDGVNDAPALAQADVGIAMGAA
                     GSDVAIEAAHIALMREDWRLVPDVLRIARRTMGVVKMNLGFTAVYNFAGLTLAALGFL
                     PPVLAAAAQSLPDIGIMGNSARLLKQK"
     gene            1976..>2597
                     /locus_tag="VN363_09780"
     CDS             1976..>2597
                     /locus_tag="VN363_09780"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:NF012478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FKBP-type peptidyl-prolyl cis-trans isomerase"
                     /protein_id="HWQ84847.1"
                     /translation="MKKQPKMSRWLVTAALSLLVMMLAACQAPASTPTEPASPAAPTQ
                     ARGETDTGTALPTSGASDSPPAAAATAMPESATPTPLVLEGAQTTASGLQYLEENAGS
                     GENPKTGEIVTIHYIVSLVDGTELANTYTMNQPASLPWGDNRLLPGWEEGVGMMKPGG
                     KATLVLPPDLAFGEEGVGGIPANSQLVIEVELLTIKPAPVPSEIEAT"
BASE COUNT          537 a          711 c          911 g          438 t
ORIGIN      
        1 gggggttgtg gtcgtgggtg agtggatggg ggtattcgag tggctaaacc agcggattcc
       61 cttcgccatc ggcctgttga tcgtgctggc aggcggatac ccggtctttg caaatgttat
      121 ccgggcgacg ctgaagcggc agataatctc acatacactg atgaccatcg gggtactcgc
      181 ggcgctggcg gtaggcgaat gggtgacggc ggcgatcgtg gtgatcttta tgcgggtggg
      241 cgattatgtg gagaatttca ccaccgaaag cgcccgccgg gcggtgaaag acctgacagc
      301 cctggcaccg caaacggcgc gggtcgaacg ggacggtgcg gaaatgaata tcccggtgga
      361 gcaggtgatc ccgggtgaga cgatcattgt ccgaccaggc gagaagatcc cggtggacgg
      421 cagcgtactt tccgggcagg caacggtgga ccaggcagct atcacgggtg aatccatgcc
      481 ggtggaagtg acggtgggag ggcaggtgta cgcagccaca ctggcaaagc tgggcagcct
      541 gcggatccgg gcggaacgca ccggggcaga caccacattc gggcgggtga tcaaaatggt
      601 ggaggaagcc gaagccaacc gcgccgaagt gcagcgtttt gccgataaat tcagcgcctg
      661 gtacttaccg gtcgtggccg ggatcgccgg gctgacattc gttttcagcc gcaaccccat
      721 cgcaacggcg gcagtcttgc tggtggcgtg ctcgtgctcg attgccctgg caacaccggt
      781 ggcgatgctg gcatccatcg gcgccagcgc caggcgcggg ctgctgatca agggcggcaa
      841 atatctggag ctgctggccc gggcggatgt actgctggtg gacaagaccg gcaccttaac
      901 cctcgggcaa ccccaaataa ccgatgttgt gctgctcaat ggcagctcgc gagccgaagc
      961 gctgggttat gccgccgcgg cggagcgatt ttccgagcac ccgttggccg aagcggtgcg
     1021 cagactggca ggcgcggaaa atattcaact ggacgaagtc gaggaattcc gggcaatccc
     1081 cggagaaggg gtgcgggctc tggtacgcgg gcaggcggta gcggtgggaa acgtgcgcct
     1141 gctgccggca ttcgcaagtc tggagactgc ccacaagctg gaaagccagg ggaagactct
     1201 gttgtatctg caggtggacg gagctccggc ggcgatcttt gctgctgcgg atacgctacg
     1261 cggggaagtt cctgccgccc tggccgcggt gcgcgagctg ggcatccgcc aggtggagct
     1321 gctgaccggg gacaacgagc gaacggcagc cgcgctgggc gagaagctgg gtgtttctta
     1381 ccgggccggg ctgctgccgc aagataagat cgccatcgtg aaggagtacc aggcgcgggg
     1441 gcatatcgtg gtgatggtgg gcgatggggt gaacgatgcc cctgccctgg ctcaggcgga
     1501 tgtggggatc gctatgggcg cggcgggatc ggacgtggcc atcgaagccg ctcacattgc
     1561 cttgatgcgg gaggattggc ggctggtgcc ggatgtcctg aggatcgctc gcaggacgat
     1621 gggagtggtg aagatgaacc tgggctttac ggcagtgtac aatttcgccg ggctgaccct
     1681 ggcagcgctg ggattcctcc caccggtgct ggcggcggct gcacaatctc tgcccgatat
     1741 tggaattatg gggaattctg ccaggctgct caagcagaag taaatgaacc ggggagcaga
     1801 agtcgaggcg aattctgctc cccgggctgg gtctggagga tcaaaaaccg ggttatgcgc
     1861 aataaacccg gtctgctgat gataaatttc taaaccaaca atccagcact tgggggtaaa
     1921 cttaggaatc ttttccccga agcgacacga aaaggggaaa cttcaggagc aaagtgtgaa
     1981 gaaacaaccg aaaatgtccc ggtggctggt aacagctgcc ctaagcctgc tggtaatgat
     2041 gctggctgcc tgccaggcgc cggcgagcac tccgaccgag ccagccagcc cggccgcccc
     2101 cacgcaagcc aggggtgaga cagacaccgg aaccgcgctg ccaaccagcg gagccagcga
     2161 cagcccgcca gccgcggctg cgacagcaat gcctgaatca gccacgccga cacccctggt
     2221 gctggaaggc gcccagacaa ctgcgagcgg cctgcagtac ctggaggaga acgccggcag
     2281 tggggagaac cccaaaacag gtgagatcgt taccatccac tatatcgtca gcctggtgga
     2341 cggcaccgag ctggcgaata cctatacaat gaaccagccg gccagcctcc cgtgggggga
     2401 taaccggctg ctgcccggat gggaagaagg ggtggggatg atgaagcccg gcggcaaggc
     2461 aaccctggtg ctgccgcccg acctggcttt tggcgaagag ggagtgggcg ggattccagc
     2521 caattcgcaa ttagtgatcg aggtggagct gctgacgatt aaacccgcac ctgtgcccag
     2581 cgagatcgag gccaccg
//
LOCUS       DATMXK010000538         3932 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_114675, whole genome shotgun sequence.
ACCESSION   DATMXK010000538 DATMXK010000000
VERSION     DATMXK010000538.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3932)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3932)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3932
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_114675"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1009
                     /locus_tag="VN363_09785"
     CDS             <1..1009
                     /locus_tag="VN363_09785"
                     /inference="COORDINATES: protein motif:HMM:NF018884.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016791 - phosphatase activity [Evidence
                     IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="SpoIIE family protein phosphatase"
                     /protein_id="HWQ84848.1"
                     /translation="YVGEISMLAPDGLRTASACTKEYTRLLVMTRSDFEGLLARRPTL
                     ATEFTRVLISRLNESHTNAIRDLQEKNVRLSRAYEDLKNAQDQLIKQERMERELQVAY
                     EIQMSILPEAMPDFAGFDFGALIVPAREVGGDFFDLLPLQDGKMGIVIGDITDKGVPA
                     AIFMAQTHALLRASASHTKSPGQTLLEINQLLLGMNTRNMFATILYGILDGQTRQFSY
                     ARAGHELPIVLKPSGEIEIGEHGVGMPVGIFEEPKIDEQTLILPPGSILLLYTDGATD
                     ALVTDERRFGMEGLLEAFRKAANLPAQEVCKTIFNSLVVEGRPELRIDDVTLVVICSL
                     D"
     gene            1056..>3932
                     /locus_tag="VN363_09790"
     CDS             1056..>3932
                     /locus_tag="VN363_09790"
                     /inference="COORDINATES: protein motif:HMM:NF024589.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ84849.1"
                     /translation="MDSLSVYVPMDRLNALIRGEALAERAEGAALFADLSGFTALTEL
                     LIQNLGPKRGAEKLLVFLDRIYEPLIIQVHNYGGSVIGFSGDAITCWFNLDTGIQAIA
                     SALAMQEEMKAFASLEILDGIEVSIGLKVAVAAGTARRFLVGEPDIQVIDVLAGSPLE
                     HLAQAEHLAARGEVLVDSATANYLTGEIEIAEWRLDPEAGEYYAVVKSLTVPFQPRPW
                     PDLEANLLDRQFLRQWLLPPIYQRLQSGMGEFLAELRPAVALFLRFGGIDYDEDDSAG
                     VKLDAYIRQVQRIIARFEGSLIQLTVGDKGSYLFAAFGAPIAHEDDANRAVLAAVELS
                     KILPVEGPGKRVQIGISEGRMRTGAYGGSSRRTYGVLGPQTNLAARLMQAAAPGQILV
                     SQLIYKATLAQYNWLELPAIQLKGVREPLPVFALNEYTDRRSIHLQEPQYNLPMVGRK
                     SELAAIEASIQKTLAGQGQIVVITGEAGMGKSRLVAESIRLCLENGLVGFGGECESYG
                     TNTSYHLWYSIWRAFFNVDPGWSVEESSTMLQDTLAQVDPSLVPRLPLLGPVLNLPFP
                     DNDLTRSLDAKRRKTLLEGLLITCLRARAHQQPLLFVLENCHWMDPLSAELAAVVARS
                     IADVPVILLLALRPVEDQENRENPLGPIKTYTAIQLTELPDEEVELLIQLKLEQIYAG
                     QTGLPSRLIQYIGKRAQGNPFYIEELLNYLRDMNINPQNSSALEQIELPESLNSLVLS
                     RIDQCTESQKITLKVASVLGRLFIAAWLWGAFPDLSRPEMVQTDLDELRRLELTSLEK
                     TEPELTYLFKHIITQEVAYESLPYATRSLLHGQIAQFIENTYPDKISQNVDLLAFHYE
                     LSFNEPKKREYLLKAGQTAQANYANQAALNYYQHLIPLIQGSERVDVMLKLGQVYELV
                     GQWDAAKNLYQSGLQIGEALEDRNLQALCQTATGELLRKRGAYPD"
BASE COUNT          930 a         1023 c         1078 g          901 t
ORIGIN      
        1 gtatgtgggt gaaattagta tgcttgcacc ggatggtctg cgtaccgcca gtgcatgcac
       61 caaagaatac acccgattgc tggtgatgac acgctcagat tttgaggggt tattggcgcg
      121 ccggcccacc ctggcgacgg agtttacccg ggtcctgatc agccggttaa acgaatcgca
      181 taccaatgcg atccgggatt tacaggagaa aaatgttcgc ctatcccggg cgtacgaaga
      241 ccttaaaaat gcccaggacc agctgattaa gcaagaacgg atggaacgcg agctgcaagt
      301 tgcctatgag atccagatga gcattctgcc ggaggcgatg ccggattttg cgggttttga
      361 tttcggcgcg ctgatcgtcc cggcacggga agtgggtgga gatttcttcg atttgctccc
      421 tctgcaagat gggaaaatgg ggatcgttat cggggatatc accgataaag gcgttccagc
      481 cgcgatcttc atggcccaga cccacgcact attacgggcg tctgccagcc acacaaaatc
      541 gcccgggcaa accttactcg agatcaacca gcttttgctc gggatgaaca cccgcaatat
      601 gttcgccacc atcctgtatg gaattttgga tggacaaacc cgccaattct cttacgcccg
      661 tgccgggcac gagcttccga tcgtgctcaa gccaagtgga gagattgaga tcggcgaaca
      721 cggtgtagga atgccggttg ggatattcga agagcccaaa atcgatgaac agacccttat
      781 ccttcctccg ggcagcatcc tgctgctcta taccgatggg gctaccgatg cactggtaac
      841 agatgagcgt cgctttggga tggaagggct gttggaggcg ttccgcaagg cagcaaactt
      901 acccgctcaa gaagtgtgta aaaccatctt caattcactg gtcgtggagg gacggcccga
      961 gctaaggatc gatgatgtaa ccctggttgt catctgctcg cttgattaat aagcacgtga
     1021 aacagcttgt gaaaaaatta aacaggtagt cggaaatgga tagtctgagc gtttatgttc
     1081 caatggatcg gctgaacgcg ctgatcagag gcgaagcgct ggctgagcgc gctgaaggtg
     1141 cggcgctttt tgccgacctt tctggattca cagccctgac agaattatta atccagaacc
     1201 tgggacccaa acgcggcgcg gaaaaactgc tggtatttct ggaccggatt tacgagcctc
     1261 tgattatcca ggttcacaat tacggtggca gcgtcatcgg ctttagcgga gatgcaatta
     1321 cctgttggtt taatctggac accggtatac aggcgatagc cagcgccctg gcaatgcaag
     1381 aagagatgaa ggctttcgct tcgctcgaga ttttagacgg tatagaagtg tctattggcc
     1441 tgaaagtagc tgtggcagcc ggaacagccc gccgcttttt agtaggtgag ccagatatcc
     1501 aggtcattga tgtcctggca ggctctccgc tggaacacct ggctcaagct gagcacctag
     1561 ctgcccgtgg tgaggtatta gttgattctg ctacagcaaa ctatctgacc ggcgagattg
     1621 agatcgctga atggcgcctt gaccctgaag ccggtgaata ttatgcagtt gtgaagtcct
     1681 tgactgtccc atttcagccg cgtccctggc ccgatttgga agctaacctg ctcgaccggc
     1741 aattcctccg gcaatggctg ctgcccccca tttaccagcg gctgcagagc gggatgggtg
     1801 aattcctggc agagctgagg cctgccgttg ccctgtttct gcggtttggt gggatcgatt
     1861 atgacgagga tgattccgct ggagttaaat tggatgccta catccgccag gtgcagcgga
     1921 taattgcccg ttttgaaggc agcttgattc aactgacagt cggcgataaa ggcagctact
     1981 tatttgctgc ctttggcgct ccgattgcac acgaagatga tgccaatcgg gcagtgttgg
     2041 cggctgtgga gctgagcaaa attctgcccg tcgaagggcc gggaaagcgt gtccagatcg
     2101 gcatcagcga agggcgcatg cgcactgggg catacggagg cagctcacgc cgcacctatg
     2161 gggttttagg cccgcagacg aacctcgcag ctcgtttgat gcaggcagct gcacccgggc
     2221 agatcctggt gagtcagttg atatacaaag caaccctggc tcagtacaac tggcttgagc
     2281 tgccggctat ccagctcaag ggtgttaggg aaccccttcc cgtgtttgcc ctgaatgagt
     2341 ataccgaccg gcgatccatc cacctgcagg aacctcagta caatctgccc atggtaggcc
     2401 gtaaatccga gctggctgcg atagaagcca gtattcagaa aacgctggcc ggacagggcc
     2461 agatcgtggt gatcactggc gaggctggga tgggtaaatc tcgcctcgtt gccgaatcaa
     2521 ttcgcctctg cctggaaaat ggcctggttg gttttggggg tgaatgtgaa tcctacggta
     2581 ccaataccag ttatcacctc tggtattcga tctggcgagc attttttaat gtagatcctg
     2641 gctggtctgt tgaggaaagc tcaaccatgc tgcaggatac gcttgctcag gtggatccca
     2701 gtttagtgcc ccgcctgcca ctcctgggac cagtattaaa cctgcctttc ccggataatg
     2761 atttaacccg gtcgctggat gccaagcggc gaaaaaccct gctggaaggc ttattgatta
     2821 cctgcctgcg cgcccgagcc caccagcagc cactgctctt cgtgcttgag aactgccact
     2881 ggatggatcc actttccgca gagctggcag ctgtggttgc tcgctcaatc gcagatgtac
     2941 cagttatatt gctgctggcg ctcaggcctg tcgaagacca ggaaaaccgg gaaaatcccc
     3001 ttggcccgat taaaacatac accgcaatcc agctgactga gctccctgac gaagaagttg
     3061 agctgttgat ccaattaaag ctggagcaaa tctatgctgg acagaccggc ctaccatcca
     3121 ggcttatcca gtatattggc aaacgcgccc agggtaatcc cttttatatt gaagagctgc
     3181 tcaattattt acgggatatg aatatcaatc cccaaaatag cagcgcattg gaacagatcg
     3241 agctccccga gagtctcaat agtctggtct tatcccggat cgaccagtgt accgaaagcc
     3301 agaagatcac gcttaaagtt gccagtgttc tgggtcgcct cttcattgct gcctggctgt
     3361 ggggcgcctt tcccgatctc agtcgtccgg agatggtcca gactgatctg gatgaacttc
     3421 gccgcctgga gctgacctct ttagaaaaaa ccgaacctga gctgacttat ttattcaagc
     3481 atatcatcac ccaggaggta gcatacgaaa gcctgccata tgccacccga tccttgctgc
     3541 atgggcagat tgcccagttc attgagaaca cctacccgga caagataagc caaaatgtgg
     3601 acctgctcgc ttttcactat gagctgagct ttaacgagcc caaaaaacgc gagtatttgc
     3661 tcaaagccgg tcaaacagcc caggcaaatt atgccaatca ggcagcacta aattattacc
     3721 agcacctgat ccctctgatt cagggaagcg aacgggttga tgtgatgctc aaactcgggc
     3781 aggtgtacga gctcgtagga cagtgggatg ctgccaaaaa cctgtatcag agcggtctac
     3841 agatcggtga ggcgcttgaa gaccgcaatc tgcaagccct gtgccagact gccaccggcg
     3901 aattattacg caaacgggga gcctacccgg at
//
LOCUS       DATMXK010000539         2082 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_165672, whole genome shotgun sequence.
ACCESSION   DATMXK010000539 DATMXK010000000
VERSION     DATMXK010000539.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2082)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2082)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2082
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_165672"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1585
                     /locus_tag="VN363_09795"
     CDS             <1..1585
                     /locus_tag="VN363_09795"
                     /inference="COORDINATES: protein motif:HMM:NF024581.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0050660 - flavin adenine dinucleotide
                     binding [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="FAD-linked oxidase C-terminal domain-containing
                     protein"
                     /protein_id="HWQ84850.1"
                     /translation="SGEHGDGLARSEWLGELLGEELLGALRLLKTAADPHNLLNPGKI
                     VSPDSAPAPRMDTSLRLGNEYQAAAWQPVFGYSQQVNIQEAIEQCNGAAVCRKDSGVM
                     CPSFQATEEEEHSTRGRANLLRAMISGQFPTQELAEKAVYEALDLCLACKGCKAECPS
                     SVDMAKLRYEFLAYYYSSQSETHARRRLRDYLFAYIGDLARIGWRFAPLVNAILRAEL
                     FKSFAESRLGLSRQHPLPVFHSPAYRMDGLPLPPRDQPVEKVLLLSDAFNEYFRPQVG
                     IGAVRLLQAAGCEVVRLPLLGAGRTLISKGFLKPAQAHARKLAAAIERADPAGTIPIV
                     GLEPSEVYTLLDEYPDLLNGNPEVKYIAERSWLIDEYLLRNSGDSVARFDRIPPATGK
                     QFSVGERILLHGHCYQKARPPAADGYPTGVEATRELLERCGYQVELIDSGCCGMAGAF
                     GYEKEHYAISHQVAELKLLPAIRKQLEDHSGRVRVCAAGVSCDAQIEDSLNISTYHPA
                     SLVGRALGIGGFEEQVVKS"
     gene            1582..>2082
                     /locus_tag="VN363_09800"
     CDS             1582..>2082
                     /locus_tag="VN363_09800"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84851.1"
                     /translation="MKARSCPYLGLEYDPSTYQAFPSGENFCHKARVPGPVDQQYQAT
                     VCLTDAYPGCPIFAGEGAALGSAGRAPALPRLSRKAFLFLLAGAVVVGLSLLALLLPG
                     AKNIGAFFSSPTDSPFPPTVTQTRIVVVEGQVTPTPENLSLPLIWRQPTGTPLPTATS
                     TQTPTVT"
BASE COUNT          434 a          578 c          631 g          439 t
ORIGIN      
        1 cagcggcgag cacggcgatg gcctggcgcg cagcgagtgg ctcggcgagc ttttggggga
       61 ggagctgttg ggcgcgctgc gcttattgaa aacagccgcc gacccgcata acctcctcaa
      121 cccgggcaag attgtttcgc cagactcagc cccggctccc agaatggaca cgagcctaag
      181 gcttggaaac gaatatcagg cagcggcgtg gcagccagtg tttggttatt cacagcaggt
      241 taacattcaa gaagcgatcg agcagtgcaa cggagcggcg gtgtgccgca aagacagcgg
      301 tgtgatgtgc ccatccttcc aggcaacgga ggaggaggag cacagtactc gcggccgggc
      361 gaacctgctg cgggcgatga tctcgggaca attccctacc caggagctgg cagaaaaagc
      421 agtctacgaa gccctcgacc tgtgcctggc atgcaagggg tgtaaggcag agtgtccatc
      481 ttccgtcgat atggctaaac tgcggtatga atttctggcg tattattaca gctcccaatc
      541 cgaaacccac gccaggcgca ggctgcgcga ttacctgttt gcctatattg gcgatctggc
      601 aaggattggc tggcggtttg cgccgctggt caatgccatc ctcagagcag agttatttaa
      661 atcgtttgca gagagccgcc tgggtttgtc ccgccagcat ccactcccgg tattccacag
      721 ccccgcctac cggatggatg gcctgcccct gcctccccga gaccagcctg tggaaaaagt
      781 tcttttgcta tcggatgcat tcaacgaata tttccggccg caggtgggca taggtgccgt
      841 caggctcctt caggcggccg gctgtgaggt ggtccggctg ccgctgctgg gtgccgggcg
      901 cacattgatc tcgaagggct ttctcaaacc cgcccaggcg catgccagaa agctggctgc
      961 ggcgatcgaa agagctgacc ccgcagggac gatcccaatt gtgggcctgg agccatccga
     1021 ggtttatacc ctgctggatg aatatccgga tttgcttaat ggtaatccgg aggtaaaata
     1081 tattgcggag cgatcctggc tgatcgacga gtatttactt cgcaacagtg gggatagtgt
     1141 ggcccggttt gaccggattc cacccgccac tggaaaacag ttcagtgtgg gggagcgaat
     1201 tcttttgcat ggacactgct accagaaggc acgtccacct gctgcggatg gatatccaac
     1261 cggagtcgaa gctacccggg agctgctcga acgctgtggt tatcaggtcg agctaatcga
     1321 cagcggctgc tgcgggatgg caggtgcgtt tggatatgaa aaagagcatt atgcgatttc
     1381 acaccaggtc gctgagctca aactgctgcc cgcgatccgg aaacagcttg aggaccattc
     1441 agggagggtg agagtgtgtg ctgcgggagt ctcctgtgat gctcaaattg aagacagttt
     1501 gaatatctcc acttaccatc ccgccagcct ggtgggtagg gcgctgggta tcggcggttt
     1561 cgaggagcaa gtcgtaaaat catgaaagcg cgcagctgtc cttatctggg actggaatac
     1621 gatccatcta cgtaccaggc ctttccatcc ggggaaaatt tttgccataa ggcgagggtc
     1681 ccgggtccgg tggatcagca ataccaggcg acggtttgcc tgaccgacgc atatccaggc
     1741 tgtccaattt ttgccggcga gggtgcagcg ctcggatcgg cagggcgcgc tcctgcgctg
     1801 cccaggcttt cgcgaaaggc gtttttgttc ttactggccg gcgcagtggt tgtcgggtta
     1861 agcctgctgg cattgctcct gccaggtgcg aagaatatcg gcgctttttt cagctcgccg
     1921 acagattcac cttttccacc gacggttaca cagaccagaa tcgtggtggt tgaggggcag
     1981 gtgacgccta ctccggaaaa tctcagccta cctctgatct ggcgccagcc aaccggcacg
     2041 ccgctgccca ccgccacctc aacccagaca ccgaccgtaa cc
//
LOCUS       DATMXK010000540         2872 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_1664, whole genome shotgun sequence.
ACCESSION   DATMXK010000540 DATMXK010000000
VERSION     DATMXK010000540.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2872)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2872)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2872
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_1664"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..770
                     /gene="lgt"
                     /locus_tag="VN363_09805"
     CDS             <1..770
                     /gene="lgt"
                     /locus_tag="VN363_09805"
                     /EC_number="2.5.1.145"
                     /inference="COORDINATES: protein motif:HMM:NF013915.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0008961 -
                     phosphatidylglycerol-prolipoprotein diacylglyceryl
                     transferase activity [Evidence IEA];
                     GO_process: GO:0009249 - protein lipoylation [Evidence
                     IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="prolipoprotein diacylglyceryl transferase"
                     /protein_id="HWQ84852.1"
                     /translation="YGLIIMGGAVLAAYLASREAKRRGYDPEIIWDALVWVLIAGIIG
                     ARIWHIFTPSPSLQAQGITTQYYLTHPLEAIWTRNSGLGIPGAVIGGALALYLFTRKR
                     GLNFWVWVDIAAPAVALGQAIGRWGNFVNQELYGSPTDLPWAVHIDPQYRLPGFENFE
                     TFHPLFLYESIWNLANFFFLIWLGRRYPDKLHAGDLFLIYLVTYPVARFLLEFIRLDS
                     AQIAGLNANQTVMAIVAVLALLALFLRHRRPAPRVAA"
     gene            888..1829
                     /locus_tag="VN363_09810"
     CDS             888..1829
                     /locus_tag="VN363_09810"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84853.1"
                     /translation="MIQTQDLSKRFADFLAVDRVTLQLGAGQVLALLGPNGAGKTTTV
                     RMLTSVLRPTAGWARVCGYDVVAQPEKVRACVGVLTEQHGLYGRMRGDEYLDFFGQIY
                     GLAGTHRSQRIHSLFDQFGMQEYAHRRIGEYSKGMRQKLALARALLHEPPVLLLDEPS
                     SAMDPESARTVRDAILELRSSERAIILCTHNLAEAEELADQIAIIRRGKIIVQGSPAE
                     LKQRVLGLQEFELKLAGPHNGTLPELPASVELTGSGPDTLRYRTSEPQQDNPEVLRAM
                     LAGGYPVMGLQEVPRSLEDVYLRVISADETQDLINAG"
     gene            1819..>2872
                     /locus_tag="VN363_09815"
     CDS             1819..>2872
                     /locus_tag="VN363_09815"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease subunit"
                     /protein_id="HWQ84854.1"
                     /translation="MQGSQPSLLGSLRAALVNTRPAWIITRREISDQLRDWRIILPVF
                     FLTIFFPGLMNFTAGQLVDFVERYNSPLIGDRLMPFLLMIVGFFPISVSLVIALESFV
                     GEKERHSIEPLLSSPLTDTQLFLGKLAAATVPPVLLSYLGIAVYLFGVYLDVSWRPPP
                     ALLLQVILLSTVQAVVMVSGAVVISTQTTSVRAANLLSSFIVVPMALLIQGESVVMFW
                     GDYSGLWWIIAGLAVIAGVLVRTGMAHFNREELLGRELDTINLRWGWGVFIEQFRGEG
                     RGLKAWYRTRVWPAARQLGIPMAMMVLAIVVSGIIGARLVELYPPPPELISLSGLNAG
                     FMEELASTTFFSTLGAG"
BASE COUNT          494 a          854 c          846 g          678 t
ORIGIN      
        1 ggtatggctt aatcattatg ggcggggcgg tgctggctgc ctacctcgcc agccgggaag
       61 ccaaacgccg ggggtacgat cccgaaatta tctgggatgc cctggtctgg gtcctgattg
      121 ccggcatcat cggcgctcgc atctggcata tttttacccc atccccttct ttgcaggccc
      181 agggaattac cacccagtat tatctcaccc accccctgga agcgatctgg acgcgcaaca
      241 gtggtttggg catccccggg gctgtgattg gcggtgcgct ggccctgtac ctcttcaccc
      301 ggaagcgcgg gctcaatttc tgggtgtggg tggatattgc tgccccggcg gtagcactgg
      361 gccaggcgat tggacgctgg gggaattttg tcaatcagga attatacggc tcgcctaccg
      421 atctgccctg ggcggtccat attgatccac agtatcgtct gccgggtttt gaaaattttg
      481 aaaccttcca ccccctcttt ctctacgaat ccatctggaa tctggccaat tttttctttc
      541 tcatctggct gggcaggcgc tacccggaca aacttcacgc cggcgatctc ttcctgatct
      601 atctggttac ctatcctgtg gcgcgcttcc tgcttgaatt tattcgcctg gattcagccc
      661 agatcgccgg gctgaacgcc aaccagaccg tgatggcgat tgttgcagtt ctggctttac
      721 tggcgctgtt cctgcgccac cgcaggcctg ctcccagggt ggctgcctga atcgcctaac
      781 cgatctgtaa tcttaagcat ttacccctgc gagtacaata cccccagcac gccgcgttca
      841 gccaggctgg caggcgctgc caaatctgtg cgtaagaatg gctggatatg atccagacac
      901 aggacctgtc caaaaggttc gctgatttcc tggcagtaga ccgggtaacc ctccagcttg
      961 gcgccgggca ggtgctggcc ctgctgggac cgaatggggc cgggaagacc accaccgtac
     1021 gtatgcttac ctcggtgctc cgtcccactg cgggctgggc gcgcgtctgc ggctatgatg
     1081 ttgtcgctca accggaaaag gtccgcgcct gtgtaggggt attgaccgag cagcacggcc
     1141 tgtacggtcg catgcgcggc gacgaatacc tggatttttt cggtcagatc tacggcctgg
     1201 ctggaaccca tcgcagccag cgcatccaca gcttatttga tcagtttggt atgcaggaat
     1261 atgcccaccg ccggatcggg gagtactcga agggtatgcg ccagaaactc gccctcgctc
     1321 gggccctgct gcacgagccg cccgtccttc tgctcgacga gccatcttcc gccatggatc
     1381 ctgagagcgc ccgcacggtc cgcgatgcaa tccttgagct gcgctcatcc gaacgcgcca
     1441 tcatcctgtg cacccataat ctggccgagg ccgaagagct ggccgatcag atcgccatta
     1501 tccgccgcgg caaaatcatt gtccagggtt ctcctgctga gcttaaacaa cgcgttttag
     1561 ggctgcagga atttgaattg aagctggctg ggccgcacaa tggtacgctc cctgaactgc
     1621 ccgcctccgt ggagctgacc ggatcgggtc ctgacacact gcgctatcgc acatccgagc
     1681 ctcagcagga taatcccgag gtgctgcggg cgatgctggc tggcggctat cccgttatgg
     1741 gtctacaaga agtcccccgc agcctggaag atgtctatct gcgggtgatc tccgcggatg
     1801 aaacccagga tctgatcaat gcagggtagc caaccctccc ttctggggag cctgcgtgct
     1861 gcgctggtta acacccgccc ggcctggatt atcacccggc gcgagatcag tgaccagctg
     1921 cgcgattggc gcatcatctt gccggttttc ttcctgacca tctttttccc cggtttaatg
     1981 aactttaccg ctggccagct ggtcgacttt gtcgaacgct acaattctcc attgatcggc
     2041 gatcgcctga tgccattttt gctgatgatc gtcggctttt tcccgatctc ggtttccctg
     2101 gtgatcgccc tggaaagttt tgtgggtgaa aaagagcgcc acagcatcga gccgctgctc
     2161 agctcgccgc tgaccgatac ccagctcttt ctgggtaagc tggctgctgc cacagtgcca
     2221 ccggttctgc tctcgtacct gggaatcgcg gtttatctct tcggggtcta cctcgacgta
     2281 agctggcgcc caccgccggc cctgctgctt caggtaatcc tgttaagtac tgttcaggcc
     2341 gtggtgatgg tgagcggggc ggtcgtgatt tccacccaga cgacttcggt cagggctgcg
     2401 aatttgctct cttcctttat tgttgtcccc atggccctgc taatccaggg ggaaagcgtg
     2461 gtcatgtttt ggggcgatta ttcgggtctg tggtggatca tcgccggtct ggctgtgatc
     2521 gctggcgtgc tggtgcgcac cgggatggcg catttcaacc gcgaagagct gctgggtcgc
     2581 gagctggata cgatcaacct gcgctggggc tggggtgtct ttattgagca gttccgaggg
     2641 gaggggcgcg gcctgaaagc atggtaccgc acccgggtct ggccggcagc ccgccagctg
     2701 ggtattccca tggcgatgat ggtgctggcg atcgtggtga gcgggatcat cggcgcccgg
     2761 ctggtagagc tgtacccgcc gccgccggaa ttgatcagcc tgagcgggtt gaatgctgga
     2821 tttatggaag agctggcctc gacgacgttt ttttccaccc tgggagccgg ca
//
LOCUS       DATMXK010000541         2092 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_166773, whole genome shotgun sequence.
ACCESSION   DATMXK010000541 DATMXK010000000
VERSION     DATMXK010000541.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2092)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2092)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2092
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166773"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1117)
                     /gene="moeB"
                     /locus_tag="VN363_09820"
     CDS             complement(<1..1117)
                     /gene="moeB"
                     /locus_tag="VN363_09820"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559816.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-synthase adenylyltransferase MoeB"
                     /protein_id="HWQ84855.1"
                     /translation="MFSSLTHAEILRYSRHLLIPEVGLEGQQRLKAASVLLIGTGGLG
                     SPVALYLAAAGVGRIGLVDYDVVDYSNLQRQVIHGTSSLGVLKVESARQRMLDVNPDI
                     EVEVYNEPFTSENAMQIAQNYDILIDGTDNFPTRYLVNDVSVLLGKPNVYGSIYRFDG
                     QVSVFDAKRGPCYRCLFPEPPPPGLVPSCAEGGVLGVLPGTIGTLQATEALKLILGIG
                     EPLIGKLMLYNALDMSFDFVNLRKNPRCKICGPEPEVTELIDYEAFCGVPGHDHEDGS
                     VGGGYDIEAVDLAKRLKQGNHLRLLDVREPHELEISHLDNATLIPLGQLAARLSELDT
                     AEEIVVFCKAGTRSARALELMVSAGFRKIKNLKGGINA"
     gene            complement(1133..1405)
                     /locus_tag="VN363_09825"
     CDS             complement(1133..1405)
                     /locus_tag="VN363_09825"
                     /inference="COORDINATES: protein motif:HMM:NF014636.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008641 - ubiquitin-like modifier
                     activating enzyme activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ubiquitin-like small modifier protein 1"
                     /protein_id="HWQ84856.1"
                     /translation="MPTLKIPTPLRPYASGQGEFTVQAETVAEALDELVSQHPALKQH
                     LFAETGELRAFVNLFLNEEDIRHLQGVQTPLGERDKLILIPSIAGG"
     gene            complement(1458..1748)
                     /locus_tag="VN363_09830"
     CDS             complement(1458..1748)
                     /locus_tag="VN363_09830"
                     /inference="COORDINATES: protein motif:HMM:NF023174.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3303 family protein"
                     /protein_id="HWQ84857.1"
                     /translation="MSLYFVQHKHREETCPAKDPAAGAMLLTHINPMNARKFGVNILG
                     DAVLDGQHTFVLIVEADELAKVENFMLPFRQAGTVDIQPASTCEVVVERAGC"
     gene            complement(1769..>2092)
                     /locus_tag="VN363_09835"
     CDS             complement(1769..>2092)
                     /locus_tag="VN363_09835"
                     /inference="COORDINATES: protein motif:HMM:NF025819.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M67 family metallopeptidase"
                     /protein_id="HWQ84858.1"
                     /translation="GQRQVETILALTNARENTARHNRYLITPQDMLLGEREAMRQGLD
                     IVGVFHSHPDHPDQPSEFDREWALPWYSYLITSVARGTAQGSRSWRLQDDRSQFVEEK
                     LAINE"
BASE COUNT          469 a          623 c          531 g          469 t
ORIGIN      
        1 aggcattaat tccacccttt aggttcttga ttttccggaa gccggcactg accattaatt
       61 ccagcgcccg tgccgagcgc gtccctgcct tgcaaaatac cacaatctcc tctgcagtat
      121 ccagttccga caggcgggct gccagctggc ccagagggat caaggtggca ttgtcgaggt
      181 gggaaatctc cagctcgtgc ggctcgcgca catccagcaa gcgcaggtga ttgccctgct
      241 tcaggcgttt tgccaggtcg accgcttcga tatcatatcc gcctcccaca ctgccgtcct
      301 catggtcatg cccgggcaca ccgcagaatg cttcataatc gatcagctcg gtgacttctg
      361 gctcaggtcc acagatcttg cagcgcggat ttttccgcaa attaacaaaa tcgaacgaca
      421 tatccagggc attatagagc atcagcttgc caatcagcgg ctcaccaatc cccaggatca
      481 gcttgagcgc ctcagtagcc tgcagggtcc cgatcgttcc gggcagaaca cccagcacac
      541 caccctccgc acaggagggg accagccccg gcggcggtgg ctcggggaag aggcagcggt
      601 agcatgggcc gcgttttgca tcaaagacgc tcacctgacc gtcgaagcga tagatcgagc
      661 catagacatt gggtttgccc agcaggacac tgacatcatt aaccagatag cgagtgggga
      721 aattatccgt gccatcgatc aggatgtcat aattctgggc aatctgcatg gcattttccg
      781 aagtgaacgg ctcgttataa acctccactt cgatatccgg atttacatcc agcatccgct
      841 ggcgggcaga ttcgactttt agcacaccca ggctgctggt gccgtggatc acctggcgtt
      901 gtaaattgga ataatctacc acatcataat ccaccagccc gatccggcct acgcccgcag
      961 ccgccaggta cagcgccacc ggtgagccca gcccgccggt tccgattaat aacaccgagg
     1021 cggctttgag cctctgctgt ccttccagcc ccacttcagg gatcaataaa tgccttgaat
     1081 aacgcaaaat ctcagcatgg gtaagtgacg aaaacataat tctccagttc ttctatccgc
     1141 cagcaatgct ggggatcagg atcagcttat ctcgctcgcc cagaggagtc tgcacgcctt
     1201 gcagatggcg gatatcctct tcgtttaaaa acaggttgac aaaagcccgc agctctcctg
     1261 tttcagcaaa aaggtgttgt ttcaaagcag gatgttgact caccaattca tctaatgcct
     1321 cggctacggt ttctgcctgg actgtaaact caccttgtcc gctggcatac ggccttaaag
     1381 gcgtgggtat ttttagggta ggcatgacta attgtaccgc tccatttctg tgtattaagt
     1441 tgcgtatcag gctcacttca gcagccagcg cgttcgacaa cgacttcaca ggtcgacgcc
     1501 ggctggatat ccaccgtccc cgcctggcgg aacggcagca tgaagttttc aaccttagcc
     1561 agctcgtccg cctcaacgat caacacgaac gtatgctggc catccaggac cgcatctccc
     1621 aggatattga cgccgaattt tcgggcgttc atcgggttaa tatgggtcaa cagcattgcc
     1681 ccggcagcgg ggtcttttgc cgggcaggtt tcttcgcgat gtttgtgttg cacaaagtac
     1741 agcgacatgg tttcctccaa cagatagctt attcattaat cgccagtttc tcttcaacaa
     1801 actggctgcg atcatcctgc aagcgccagg agcggctgcc ttgcgccgta ccacgcgcca
     1861 cggatgtaat cagataagag taccagggca gcgcccactc ccggtcaaac tctgaaggct
     1921 ggtcaggatg gtccgggtgg gaatggaaca cacccacgat atccagcccc tggcgcatcg
     1981 cttcccgctc gcccagcagc atgtcctggg gagtgatcag ataacggttg tgacgggcgg
     2041 tattttcacg cgcattggtc aacgccagaa tcgtctctac ctggcgctgc cc
//
LOCUS       DATMXK010000542         2234 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_62917, whole genome shotgun sequence.
ACCESSION   DATMXK010000542 DATMXK010000000
VERSION     DATMXK010000542.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2234)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2234)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2234
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_62917"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..>2234)
                     /locus_tag="VN363_09840"
     CDS             complement(<1..>2234)
                     /locus_tag="VN363_09840"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84859.1"
                     /translation="AFFITRWKAGKNPAHVTGQIPFLFSLMALLAVGVTQVIAANELP
                     SGLMPYQSLPARTLIYLVLGGLAAFSLTWLALVDEEWAAGGAALAGAGLYSSVIFFRV
                     SAVPEVGYAWGVLAGFGILALLSLREVTVRLAPGEGAFWKWAATFGVAQVIAAALSPV
                     PHQSINYVVYILVLICLAGLVSLKLRSQPALDRAVGLVVLLGAGLPVVFALIKSLDAV
                     FDFGLAAAAVFRLHPSEMGGANLLARSLVVAAPFGLSLLFSTPAQGRWQGIRKIVLIL
                     LQIAILLVILYARSFEGFFAWLVVLGIFGLVAGWPAIRAAWSRISALLPARIALIGLA
                     GLLVVGLVYGGIRLGYSVNPYSFNGRFGHWIGALTAFTQHPLFGGGPDNEYLYTRYAA
                     GVSLFGDAPEFIDDPLYMIRYRSGILRVHAHNLILETAAFSGLAGVITGGGMLVALIW
                     MGLKALQNAGPRLRILLAACLGGILGELAWGLLDVIRESPPFFSFPIWAVVGMTLAAA
                     RLSQPDGATGRENIHFKVGSKSSLGMIVLAVLVVLLPSMAANRYSSGFLAYQEHRWEA
                     AAQNFHAAARLNPLSAQYYWMLSKSELELGEFDQAARHLDQAIGLKRGFSPYLSQAGW
                     LAWLNGDLEAAERYFEDAIAGDPLEGWTSGLHASLGMLKVFQGQQSEAVGYLAQSFAY
                     HPELAAGADWIRLQSPEASIEVSLAPAYAQANMSLDLQRRIYAHLGMADITPRHFEQP
                     ADLRA"
BASE COUNT          595 a          689 c          589 g          361 t
ORIGIN      
        1 gccctcaggt ctgcgggctg ctcaaaatgc cggggggtga tatccgccat acccaggtga
       61 gcatagatcc gccgctgcaa atccaaactc atattggctt gagcgtaagc aggagcgagg
      121 ctgacttcta tgctggcttc aggtgattgc agtcggatcc aatccgcccc agcagccagc
      181 tctggatgat aggcgaatga ttgggccaga tatccaactg cttccgactg ctgtccttga
      241 aagactttga gcatacccag gctggcatgt agccctgacg tccatccctc cagagggtcg
      301 ccggcgatgg catcctcgaa ataacgttcg gcggcttcca gatcgccatt cagccaggcc
      361 agccagcccg cttgggataa atacggggaa aaaccacgct tcagaccaat cgcctgatcg
      421 agatgccgag cagcttgatc aaattcgccg agctcaagct cacttttcga gagcatccag
      481 taatattggg ctgaaagtgg atttaacctg gcagcagcat gaaaattctg agcggctgcc
      541 tcccagcgat gttcttgata agccagaaat ccagagctgt atcgattggc tgccattgac
      601 ggcaggagca ctaccaggac agccaggacg atcattccaa gtgaactctt ggaaccgacc
      661 ttgaaatgaa tattttctct cccagttgcg ccgtcgggtt gagacaaacg cgcagccgcc
      721 agggtcattc ccaccacggc ccagattgga aagctgaaaa atggaggcga ctcgcggatt
      781 acatccagca agccccacgc caactcgcct aatattcctc ccaggcaggc cgccagaaga
      841 atccgcagcc tgggaccagc attttgcagg gctttgagac ccatccagat caaagccacc
      901 agcatcccac ccccagtgat cacaccggct aaaccgctaa acgctgcagt ctccaggata
      961 agattatggg catgtactct cagaataccc gagcgatagc ggatcatgta gagcggatca
     1021 tcgatgaact ctggcgcatc tccaaacagg gatacacccg cagcataacg ggtgtacagg
     1081 tattcattat ccggaccacc cccaaagagc gggtgttgag taaaagccgt cagcgccccg
     1141 atccaatgcc cgaagcggcc attgaaggag tatgggttga ccgaataacc caaacggatc
     1201 ccaccgtaaa ccaggcccac gaccaacagg ccggccagcc caatcagcgc aatccgggcg
     1261 ggtagaagcg cagaaattcg ggaccaggca gcccgaatgg ccggccagcc agcaaccagc
     1321 ccaaaaatac cgagcacaac cagccaggca aagaacccct caaaggagcg ggcatacagg
     1381 atcaccagca ggatagcgat ctgcagaagg ataaggacga tcttgcgaat cccttgccag
     1441 cgcccctgtg ccggagtaga gaaaagcaag ctcaggccga aaggtgctgc caccaccaac
     1501 gaacgggcga gaaggttcgc ccctcccatc tcactgggat gcagccggaa tactgccgca
     1561 gctgccagcc caaaatcgaa cacggcatcc aggctcttga tcagtgcaaa aaccacaggc
     1621 agtccagccc ccagaaggac caccaggccc acagcacggt ccagcgcagg ctgtgagcgt
     1681 aatttgagcg agaccaatcc cgccaggcag atcaacacca gaatgtagac aacgtaatta
     1741 atgctttgat gtggcacagg cgagagcgcg gctgcaataa cctgagcgac cccaaaagtc
     1801 gcagcccatt tccaaaaagc cccttcaccg ggggctaacc tgaccgtcac ctcacgcagc
     1861 gaaaggagcg ccagaatgcc gaaacctgcc agtacacccc aggcataccc gacttcggga
     1921 acagctgaga cacggaagaa gatgaccgag ctgtataagc cagcccctgc cagcgctgcg
     1981 ccgcctgccg cccactcctc atccaccagc gccagccagg tgagactaaa cgccgctaga
     2041 ccacccagga ccaggtagat cagcgtgcgc gccggcagcg attggtaagg catcaaaccc
     2101 gacggcagct cattggctgc gatcacctgg gttacgccca cggccagcag ggccatcagc
     2161 gagaacagaa atggaatctg gccagtgaca tgagccgggt tcttgccagc cttccaacgt
     2221 gtgatgaaga aggc
//
LOCUS       DATMXK010000543         5146 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_4205, whole genome shotgun sequence.
ACCESSION   DATMXK010000543 DATMXK010000000
VERSION     DATMXK010000543.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5146)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5146)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5146
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_4205"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(267..845)
                     /locus_tag="VN363_09845"
     CDS             complement(267..845)
                     /locus_tag="VN363_09845"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558958.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit 3"
                     /protein_id="HWQ84860.1"
                     /translation="MTTEILPLEDYRKKSATNRLGLWLFILSDIFLFGGLYVSRFYLL
                     GTAQRPDVSQVLGVGVTAMLLVSSFFMNRAETAIRFGDQKTFLRSTFMTLVLGVLFLV
                     GVVGVEWQIAPGGPADGVAWSLFYAMTGYHALHVLSGVIFLFVVYRNGQNGMYSAERH
                     WQVEAAAVYWHFVDVAWIFFYPALYLIGTPVI"
     gene            complement(849..1061)
                     /locus_tag="VN363_09850"
     CDS             complement(849..1061)
                     /locus_tag="VN363_09850"
                     /inference="COORDINATES: protein motif:HMM:NF015582.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome C oxidase subunit IV family protein"
                     /protein_id="HWQ84861.1"
                     /translation="MEPEAKKHAAYMVGIGVMILLAGLTIGEYFMGAFASAWALPILL
                     VAAVKAYFVVRDYMHIGRVFAGDDEE"
     gene            complement(1091..3034)
                     /locus_tag="VN363_09855"
     CDS             complement(1091..3034)
                     /locus_tag="VN363_09855"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558960.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA];
                     GO_process: GO:0009060 - aerobic respiration [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cbb3-type cytochrome c oxidase subunit I"
                     /protein_id="HWQ84862.1"
                     /translation="MNETKKAGFLGLALVRGLMFMLIGGILGTALVSAIRLMLGLSVT
                     NPATFGFTEPAWVVGALFGAVGFMVGTGVMSDWFKWMGGKATGEHPHDDFPSGAARYL
                     SVSYDHKVIAIQYGVTSLIMMLVAGLFAIIFRTELIAPELQFLQLNDFNTLMSLHGIT
                     MIFSILLGVAAMSNFLVPLLIGANDMAFPRLNAFAYWLNVPAGIILLSSLFLGGFDTG
                     WTGYPPLSTRAPLGMQMFFLGVYLFGLSSIFSSLNIIATIIRLRAPGMTYFRMPIFVW
                     TAFATSIISLTATQLIGLSFQMVMFERLFGMGFFNPALGGDPILFQHLFWFYSHPAVY
                     VFVLTGLGVISELLPVFSRKPLFGYRWVAMSAFGIALVGFLVWAHHMFTSGMAPYLRV
                     PFMYSTLLVAVPTGVKFFSWVATIWQGKLSFETPMLFVLGAISIFLLGGLSGPPNGTV
                     STDLHLQDTYWIVGHFHATMFGGFVFPFFAALYYWFPKLTGRMYNERLGKLHFFLMLP
                     AFYVQSLGQMQVGLLGMRRRIADYDPALGVQDTHLLITIAGYVIGISVLIAVVNYIYS
                     AKRGPVAVANPWRSRSPEFQLPSPIPVVNYATPFTVIGEPYDYGLEGSAYVSIDKERA
                     PQASPPPAPPAQAAPTADPAGAD"
     gene            complement(3112..4572)
                     /gene="coxB"
                     /locus_tag="VN363_09860"
     CDS             complement(3112..4572)
                     /gene="coxB"
                     /locus_tag="VN363_09860"
                     /EC_number="7.1.1.9"
                     /inference="COORDINATES: protein motif:HMM:TIGR02866.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004129 - cytochrome-c oxidase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c oxidase subunit II"
                     /protein_id="HWQ84863.1"
                     /translation="MPAPESQKRRVLIASSHPLFGQGLRSLLQARQTTGVEVVGMVAT
                     LEQALEALERLNPDLILVDYDDERLNRDEFLARFVEGEKKLRVVLLSLQGSSDALVYD
                     RRTLAASQIDDWLDDWKFTEDISETPGAIIPAPPAARTARLNEDRRNPMKHLVTAGLL
                     VVAVAAALIFALEKITLLPIQAADQARPIDSLFRLEFMVIAFLFALIVVFMVYSMIVF
                     RRKKGDTTDAAHIEGNTRLEIMWTVAPLVTVLVFAYLGGQSLAETVRADPRPMEVNVI
                     GQQWSWRFEYPEWGIVSTELNLPANKQALLRLWSTDVIHSFWVPEFRVKQDALPGGEA
                     NVRELRVTPNLPGEYTLACAELCGIQHAYMTAKVNVMSQADFDAWVVSLTGEPSGDPV
                     ERGSQLVTQYGCIACHSLDGTVIVGPSWKGIYGHQVTLTDGTTAEVNDEYLRESILNP
                     GARLVQGFQNLMPATYRDQLTDEQIADIIEFIKSLR"
     gene            4794..>5146
                     /locus_tag="VN363_09865"
     CDS             4794..>5146
                     /locus_tag="VN363_09865"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84864.1"
                     /translation="MPTITHQLLQEYQNDTFRLKPERRLHSADDAVAFVNERGFAFFW
                     PISGVRLPSVWAAVAGDRPVPNEHDDPGHVTWGWKDELLGQRRWYYARVVRKKATMIA
                     LDVAPHFYALSENYGS"
BASE COUNT         1302 a         1471 c         1374 g          999 t
ORIGIN      
        1 ggtaaggact cccgtgctgg tcggtcagtg taaaatcaac tgccggcagc gccgggtcga
       61 ttaaggaacc ctggtagcga taggattgtt ggacgatctg ccagcccccc agcattaacg
      121 ccactccgat aacgatccca atccccacca ggaaaaacct gtttttcatg cctgccacac
      181 tttctcagcc agggcggctt attcgccgga gcagtctgga tctggtctcc ccggatccag
      241 gtgagtgccc cgacaatcag ccaccattaa atgaccggtg ttccgatcag gtataacgcc
      301 ggatagaaaa agatccaggc gacatccaca aaatgccagt aaacggccgc ggcttccacc
      361 tgccagtgac gttcggcgct gtacatccca ttctgcccgt tgcgataaac aacgaacagg
      421 aaaatgacac ccgagagcac atgcaaagca tgataaccgg tcatggcgta aaacagcgac
      481 cacgccacgc catcggctgg accgcctggg gcgatctgcc attcaacgcc gaccacacct
      541 accaggaaga gcacgcccag gaccagggtc atgaaagtgg agcggagaaa agttttctga
      601 tccccgaagc ggatggcggt ttcagcccgg ttcataaaaa agctgctcac caggagcatc
      661 gcggttacac caacgcccag gacctggctt acatctggac gctgggcagt ccccaacaga
      721 tagaagcggg agacatacaa cccaccaaac aagaatatat cggagaggat aaacaaccat
      781 aagccaaggc ggttggtggc cgatttcttg cgataatctt caagaggtaa aatttcggtt
      841 gtcatcgctt attcctcatc atccccggca aatacccgcc caatatgcat atagtcgcgt
      901 accacaaagt aggctttgac tgccgcgacc aataatatgg gcagcgccca ggcgctggca
      961 aaagcgccca tgaaatactc accaatcgtc aggccggcga gcaggatcat caccccgatc
     1021 ccgaccatat aggcggcgtg ttttttagct tcaggttcca tggtcatatt tctcctatgt
     1081 tctgtatgga ctaatcagcg ccagccgggt cggcagttgg cgcggcctga gccggtggtg
     1141 cgggcggtgg gcttgcctga ggggcgcgtt ctttgtcgat actcacataa gccgagcctt
     1201 ccaggccata gtcgtacggc tcgccaatca ccgtgaaagg cgtcgcgtaa ttgacaaccg
     1261 ggatcggcga agggagctgg aattcaggcg agcgcgagcg ccaggggtta gcaaccgcca
     1321 cagggcctcg ttttgcactg taaatgtaat ttacgacggc aatcagcacc gatatcccaa
     1381 tcacataccc ggcaatggtg atcagtaaat gggtgtcctg cactcccaga gcagggtcat
     1441 aatcggcaat ccggcggcgc ataccgagca gccccacctg catctgcccc agcgactgca
     1501 cgtagaacgc cggcagcatc aagaagaaat gcagcttacc gagcctctcg ttgtacatgc
     1561 gcccggtgag cttggggaac cagtaataca gggctgcaaa gaatgggaag acgaatccgc
     1621 cgaacatggt ggcatggaag tgccctacga tccaataagt gtcctgcagg tgcaggtcgg
     1681 tggagacggt gccgttcggc gggccgctca gcccacccag caggaagatc gagatcgctc
     1741 ccagcacgaa cagcatgggc gtctcaaacg acagcttgcc ctgccagatc gttgccaccc
     1801 aggagaagaa tttcaccccg gtgggaactg cgaccagtaa tgtgctgtac ataaacggca
     1861 cacgtaagta gggcgccatg cctgatgtga acatatggtg tgcccacacc aggaagccta
     1921 ccagtgcaat cccgaatgcg gacatcgcca cccaccggta gccaaagagc ggcttgcgcg
     1981 agaaaaccgg gagtaactcg gaaatcactc ccagaccggt gaggacaaag acatacaccg
     2041 ccggatgaga gtaaaaccag aatagatgct ggaacaagat cggatccccg cctagggcag
     2101 gattgaaaaa gcccatccca aacaaacgct cgaacatcac catctgaaag gacaaaccga
     2161 tcagctgagt agccgtcagg ctgatgatgg aggtggcgaa agccgtccag acgaagatcg
     2221 gcatgcggaa ataggtcatg ccgggagcac gcaagcggat tatggtggcg atgatattca
     2281 gactgctaaa gatggaggaa agaccgaaca ggtatacccc caggaaaaac atctgcatcc
     2341 ccaacggcgc ccgggtgctc aggggcgggt agccagtcca accggtatca aacccaccca
     2401 ggaaaaggct gctgagcagg ataatccctg cgggcacatt gagccagtag gcgaaggcgt
     2461 tcagacgtgg aaaagccata tcattggccc caatcagaag cgggaccagg aagttagaca
     2521 tggcggcgac gcccaacaga atgctgaaga tcattgtgat gccatgcagg ctcatcagcg
     2581 tgttaaagtc attgagctga agaaactgca gctcgggagc gatcagctct gtgcggaaaa
     2641 tgatcgcaaa gagccccgca accagcatca tgattaaaga ggtaaccccg tactggatgg
     2701 cgattacttt gtggtcataa ctcacgctca ggtagcgggc tgctccagac ggaaaatcat
     2761 catggggatg ctcaccggtt gccttacccc ccatccattt gaaccagtcg gacatcactc
     2821 ccgtcccgac cataaaaccg actgcgccaa ataaagcccc caccacccag gcaggctcgg
     2881 taaagccaaa agtggctgga ttcgtgacag aaagccccag catcaggcgg attgcgctga
     2941 ccagtgccgt gcctaatatc ccgccaatca gcatgaacat gagaccacgc accagggcca
     3001 ggcctaaaaa tccagctttc ttcgtttcat tcatctgctc actcctcctg ttgatcgtcc
     3061 ggtgaccgca acggatatga tcaccggctc agtatcagga cagccagcgg cttaccgcag
     3121 gctcttgatg aattcgataa tatcggcgat ctgctcgtcc gtaagttgat cgcggtaggt
     3181 agccggcatt agattctgaa aaccctgaac cagcctggca ccgggattaa ggatcgattc
     3241 tctcagatac tcatcattta cttctgccgt agtaccatcg gtcagagtga cctggtgacc
     3301 ataaatgcct ttccagctcg ggccaacaat gacagttcca tccaggctgt ggcaggcgat
     3361 acaaccatac tgggtgacca gctgtgagcc gcgttcaacc ggatcgccgg aaggctcgcc
     3421 ggtcagcgaa accacccagg catcaaaatc cgcttgagac atcacattta ctttggcggt
     3481 catataggca tgctggatac cgcacagctc ggcgcaagcc agagtgtatt ctccgggtaa
     3541 gttgggagtt acgcgcagct cacgcacatt cgcttcaccg ccaggcaggg catcctgttt
     3601 tacgcggaat tcaggtaccc agaaggaatg gatcacatcg gtggaccaca gccgcagcaa
     3661 agcctgctta ttggcaggga ggtttagttc cgtgctgaca atcccccatt ctggatactc
     3721 aaaacgccag gaccactgct gcccaatgac atttacttcc atgggacggg ggtcagcgcg
     3781 caccgtttct gccaacgact gcccacccag ataagcaaat acgaggactg tcaccagcgg
     3841 cgctacggtc cacatgatct cgaggcgcgt attcccttca atatgggcag cgtcggtggt
     3901 gtcacccttt ttccggcgga agacgatcat gctgtacacc atgaagacaa caatcagggc
     3961 aaacaaaaaa gcaatgacca taaactccag ccggaacagg ctgtcaatgg gccgcgcctg
     4021 atcggctgcc tggatgggta gcaaagtaat cttttccaga gcaaagatca atgcagccgc
     4081 cacagcgacg accagcaatc ctgcagtgac cagatgtttc atagggtttc tcctgtcctc
     4141 attcagtctc gccgtccggg cggcaggggg agccgggatt atcgctcctg gcgtttcaga
     4201 aatatcctca gtgaatttcc aatcatccag ccaatcatcg atttgggagg ccgccagggt
     4261 gcgccggtca tagacgaggg catcactgct gccttgcagc gacagcagca ccacgcgcag
     4321 ctttttctcc ccttccacaa accgggccag gaattcgtcc cgattcagtc gctcatcatc
     4381 gtagtctacg agtatcaggt ccgggtttaa gcgctccagc gcctctaaag cctgctcgag
     4441 ggttgcgacc atcccgacca cttctacccc ggttgtctgg cgcgcctgca gcaggctgcg
     4501 caagccttgc ccgaacagcg gatggctgga ggcgatcaga acacggcgct tttgagattc
     4561 tggggcaggc atcgggcaga gcaaactcca aatcaaggat aagagaccga atataatata
     4621 ccaatattgc tccagattat catcattccc tgggtgacga ttgggtgaat tctgcctctc
     4681 caaaagggtg attcctggat cacccttttg acatgatctg gcccattttt agcaaaggtt
     4741 gcgtggtata acagacctgt ctcaaccact ctgagacagg tacggtctaa gagatgccaa
     4801 caattaccca tcaactcctt caagaatatc aaaacgacac cttccggctc aagccggagc
     4861 gccgcctgca ctctgccgat gatgcagtcg cgtttgtcaa tgagcgcggg tttgcttttt
     4921 tctggccgat cagtggggta cgcctgccaa gtgtgtgggc tgcggtcgcc ggagaccggc
     4981 ctgttcccaa tgagcatgat gacccggggc atgtaacctg gggctggaaa gatgagctgc
     5041 ttggtcagag gcgctggtat tatgccaggg tggtgcgcaa gaaagccacc atgatcgcgc
     5101 tcgacgtggc tccccatttc tatgcccttt ccgaaaatta cggctc
//
LOCUS       DATMXK010000544         2251 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_5618, whole genome shotgun sequence.
ACCESSION   DATMXK010000544 DATMXK010000000
VERSION     DATMXK010000544.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2251)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2251)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2251
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5618"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..513)
                     /locus_tag="VN363_09870"
     CDS             complement(<1..513)
                     /locus_tag="VN363_09870"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF012709.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit J"
                     /protein_id="HWQ84865.1"
                     /translation="MTALQVIFILVAAATLGAALMTVIVGNMIHAALWLIVSLFGIAV
                     VYVLLNAGFLAVAQVVIYIGAIAILVIFAIMLTRSVAVDQGTQINSNWGWGAVLALAL
                     FGALVWVLSSWPGINTGAQALPVGQDTVLQLGEALLAPNQYLLVFEVASVLLLAALIG
                     SIMIARVRKES"
     gene            complement(513..1733)
                     /gene="nuoH"
                     /locus_tag="VN363_09875"
     CDS             complement(513..1733)
                     /gene="nuoH"
                     /locus_tag="VN363_09875"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: protein motif:HMM:NF004741.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit NuoH"
                     /protein_id="HWQ84866.1"
                     /translation="MDPIVLLANWFEGVLVGWGAPPWAVNLILSVLGIALLCTVMLLI
                     AIWCVWVERKVVARFQDRLGPNRIGPYGLFQPFTDIIKLLIKEDIEPKGIDKVPYNIA
                     PILALATVLTLWAIIPLSPQVLGSDINVGVLFIVAIGAISTLGIIMAGWSCNNKFALL
                     GALREVATMISYEVPMVIALMIPVILASSMGINKIVESQSPLPYIFVIPLAAVIFLIC
                     SQAELGRAPFDLSEAESEIVAGYHIEYSGMKFGMFYAGELLHAFTVGAIFSSLFLGGW
                     SGPFVDRVPVLGVFYLFIKSLFIYWVIMWIKYTLPRLRIDHMLSFNWKFLTPLSLALL
                     MVVAVLHKLLQNANPWVYILGMLVGNIAVGAIALAISRRQVKVERKEFQTNRSVARPP
                     ESSPTAPIPDSGGD"
     gene            complement(1832..2191)
                     /gene="ndhC"
                     /locus_tag="VN363_09880"
     CDS             complement(1832..2191)
                     /gene="ndhC"
                     /locus_tag="VN363_09880"
                     /EC_number="1.6.5.9"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559465.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008137 - NADH dehydrogenase (ubiquinone)
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="NADH-quinone oxidoreductase subunit A"
                     /protein_id="HWQ84867.1"
                     /translation="MIEQQWLYIGLFLIIAPVFPLLALTVPRFIAPKKPNPIKLQPYE
                     CGMETVGESWVQFKVQYYIYALVFLVFDVEIVFLYPWAVALDILPLFAVIEGIIFIMI
                     LVGGLFYAWKKGVLEWY"
BASE COUNT          675 a          587 c          509 g          480 t
ORIGIN      
        1 gctttctttt cggacgcggg cgatcataat cgaaccgatc aaagccgcca acaataaaac
       61 cgatgcgacc tcaaaaacta ataagtattg attcggtgct aataatgctt ccccaagctg
      121 caaaaccgtg tcctgaccta ctggcagtgc ctgcgcaccg gtattaatcc ctggccagga
      181 gctcaagacc cataccagag cgccaaataa ggcaagcgca agcaccgctc cccagcccca
      241 attagaattg atctgcgtac cctggtcaac ggctacgcta cgtgtaagca tgatggcaaa
      301 aattaccagg attgctatcg ccccgatgta gattactacc tgggcgacag ccaaaaatcc
      361 cgcattcaac aatacataga ccacggcaat cccgaacaag gatacgatca accagagggc
      421 agcatggatc atgttgccca cgatgaccgt catcagagca gcccccagag tcgcggctgc
      481 gaccagtata aaaataacct gcaaagctgt cattaatcgc ctccagaatc aggaattggc
      541 gctgtaggtg aactttcagg tgggcgggca acagagcggt tggtctggaa ttctttccgt
      601 tcaactttta cctggcgacg actgatcgca agcgcaatcg cacccactgc aatatttccc
      661 accagcatcc ccaggatgta tacccaggga ttcgcatttt gcagcagttt gtgcagtaca
      721 gcgacaacca tcagcagcgc cagtgagagg ggtgtaagga atttccagtt gaaactcagc
      781 atatgatcga tgcgcaagcg gggtaaagtg tatttgatcc acataattac ccagtatata
      841 aacagggatt tgataaacag ataaaacaca cccagaaccg gtacacgatc tacgaatgga
      901 ccactccatc cgcccaggaa aagcgaggaa aagatggcgc ctacggtaaa ggcatgcagg
      961 agctctccag catagaacat gccgaatttc ataccgctgt actcgatatg atagcctgcc
     1021 acaatttccg attccgcctc actcaggtca aaaggcgctc taccgagctc agcctgggag
     1081 caaatcagga aaattacagc cgccagaggg attacaaaga tatacggcag gggcgactgg
     1141 ctttcaacaa ttttattaat gcccatcgat gaagccagga tgaccgggat catcagagca
     1201 atgaccatgg ggacttcata tgagatcatc gtcgcgactt cacgcaaagc acccaatagt
     1261 gcaaatttgt tattgcaaga ccagcctgcc ataataatac ccagcgtgct gatcgcgccg
     1321 atggctacaa tgaacaggac gcccacattg atatctgaac ctaaaacctg cggtgaaagc
     1381 gggatgatcg cccataatgt cagcactgtc gctaacgcca ggattggagc gatgttgtaa
     1441 ggaactttgt cgattccttt gggttcaata tcttctttaa tcagcagctt gataatgtcg
     1501 gtaaaaggct ggaataaacc gtagggtcca attcgattcg ggcccaggcg atcctggaaa
     1561 cgagctacga ctttacgctc cacccataca caccagatcg cgatcaggag cattactgtg
     1621 cacagcaggg caatccctaa cacggacagg atgaggttca ctgcccatgg aggagctccc
     1681 caccctacca gcacaccttc aaaccaattg gcaagaagaa caattggatc cattattgaa
     1741 tgtctcctct agcagttagt cagaaaccag cgacacataa caaaggctga gggatcagcg
     1801 acctcagcct ttggcggtat tattcgaatt cttaatacca ctccaggacg cccttcttcc
     1861 aggcataaaa taacccgccc acgaggatca tgataaatat gatcccttca atcactgcaa
     1921 acaagggcaa gatatccaaa gcaactgccc acgggtataa aaacacaatc tcgacatcga
     1981 agaccagaaa aacgagcgcg tagatgtagt attgaacttt aaactggacc caggattccc
     2041 ccactgtttc catgccgcat tcgtagggtt ggagtttgat gggatttggt tttttaggtg
     2101 ctataaagcg gggaacagtt aatgccagca gtgggaatac cggagcaata attaaaaaca
     2161 agccaatata taaccattgt tgttcaatca tgggctctcc ttgccaggat taatcctcag
     2221 aacatcggta aataccacta cagtcgatac a
//
LOCUS       DATMXK010000545         3530 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_7695, whole genome shotgun sequence.
ACCESSION   DATMXK010000545 DATMXK010000000
VERSION     DATMXK010000545.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3530)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3530)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3530
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_7695"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..591)
                     /locus_tag="VN363_09885"
     CDS             complement(<1..591)
                     /locus_tag="VN363_09885"
                     /inference="COORDINATES: protein motif:HMM:NF012235.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84868.1"
                     /translation="MTEYFEIKNLSVGFRTFEGIRDVLQIESLRIQRGETYGLVGESG
                     AGKTVLALTILGLLPKPAGIIHSGEIWLDGQDLLKKSDHEMYTQYRGKRITMIFQDPM
                     STLNPVFTVGDQLARVIEKQQHLKGEARTKKALEMLEIVKLPDAARMLEKYPHELSGG
                     QRQRIIIAIALSCGAEFLIADEPTRNLDVTIQAGILK"
     gene            complement(602..1495)
                     /gene="nikC"
                     /locus_tag="VN363_09890"
     CDS             complement(602..1495)
                     /gene="nikC"
                     /locus_tag="VN363_09890"
                     /inference="COORDINATES: protein motif:HMM:NF045474.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0043190 - ATP-binding cassette (ABC)
                     transporter complex [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nickel transporter permease"
                     /protein_id="HWQ84869.1"
                     /translation="MQWIMDTRKTLQPSLREFRLSFFLLTRNKLQLASLTIIILLILV
                     AVFAPIIAPYPGHAISETNPVDKLQAPSAKYLFGTDELGRDMLSRVIYGTRISLQTAL
                     IAVGLALLIGVPLGAIAGGMGGIVDEVIMRITDVFLSFPPLLLAIAIAAFLGPNLRNA
                     MLAIAISWWPWYTRIIRGQAVSIRERQFVRAARAIGTPKSQIIFKHILPNTFAPVIVQ
                     ASMDIGGVILTIASLSFLGLGAQPPTPEWGLLISTSRTYFLNAWWYSTFPGIAIFITV
                     LVFNLIGDGFREVLDPKTRKF"
     gene            complement(1508..2518)
                     /locus_tag="VN363_09895"
     CDS             complement(1508..2518)
                     /locus_tag="VN363_09895"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84870.1"
                     /translation="MKKFILRRLILGIIVMLGVALITFFLTRVIPSDPAAKWVGPRAT
                     PEQIAKATIDLGLDQPLYVQFVRYMGDLLQGNLGASLRTRRPIMLELQENLPATIELV
                     FISTVAAVFIGIPLGVISAKRKDHLVDHISRLFSVGVVSLPTFWVGLILQLLFFRTLH
                     LFPMGGQLSTEIKLTMEIPKLTGFLMFDSLVTGNFRIFFDALWHIVLPGITIGLYPVG
                     LVARMTRSALLEILNEDYITAARSYGLPERIVLWSYALKNSIGSTSTVVALSIGYTLV
                     NTFLTESIFSWPGIGSYIATAVIGLDYPAIMGVTIFSAFSYVILNLVADIIIAMDPRV
                     RA"
     gene            complement(2618..>3530)
                     /locus_tag="VN363_09900"
     CDS             complement(2618..>3530)
                     /locus_tag="VN363_09900"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ84871.1"
                     /translation="EKFFSQVVFKKISETATRRQMIEAGEATIAVELPYSDVNALKEN
                     PAVKVNVEPSWQNLIGMFNTQKPPLDNKAVRQALAYAFPYDQVISAAVGGYARQAVGM
                     VPFGMWGYSESLPQYKYDFEKAQQLLTEAGVPAGQKLLLTYTAGNETEKSTAELYKSE
                     LAKLNIDLEIRSMPWDSQWEMAKDPDPTQRQDIFVMYWWPDLPSPYSFLYSTFHTEAE
                     PLFNLSYYYNPEFDTLIDQANEVTANDPAQAEQMFVDAQKILLDDAASLFIYDRQDVW
                     VTVANLQGFSFNPAYPTVMFFHDMYLQ"
BASE COUNT          832 a          929 c         1040 g          729 t
ORIGIN      
        1 cttgaggatg cctgcctgga tggtcacatc caggttgcgg gtgggctcat ccgcgatgag
       61 gaattccgcc ccacacgaga gggcgatggc gatgatgatg cgctggcgct ggcctccact
      121 gagctcgtgg ggatattttt ccagcatccg cgccgcatcc ggaagcttga caatctccag
      181 catctcgagc gctttcttcg ttctggcttc acccttgaga tgctgctgtt tttcgatcac
      241 tcgggcaagc tggtcaccca cagtgaagac cggattgagg gtcgacatgg ggtcctggaa
      301 aatcatcgtg atgcgcttac cccggtattg ggtgtacatc tcgtggtcgc ttttcttcag
      361 caaatcctgc ccatccagcc agatctcgcc gctgtggata atccctgccg gcttgggcag
      421 caaacccagg atcgtcaggg ccaggacagt cttgccagcg ccgctctcac ccaccagccc
      481 gtaggtctcg ccgcgctgaa tacgcaggct ctcgatttgg agcacatccc ggatcccctc
      541 gaaagtgcga aaacccactg acagattctt gatctcaaag tattcggtca tggcatttca
      601 ctcagaactt acgcgtcttg gggtcgagca cttcgcgaaa accatcgccg atcaggttga
      661 aaaccaggac ggtgataaat atggcaattc cggggaaggt gctgtaccac caggcattca
      721 ggaagtaagt gcggctggtg gagatcaaca aaccccattc gggggtgggg ggctgggctc
      781 ccagcccgag gaagctcaag gaagcaatgg tcaggatcac gccgccgatg tccatagaag
      841 cctgcacgat cacgggagcg aatgtattcg gcaggatgtg cttgaagatg atctgcgatt
      901 tgggtgtgcc gatcgcccgg gcggcgcgca caaactgccg ctcgcggatc gatactgcct
      961 ggccgcggat aatgcgcgta taccagggcc accacgaaat ggcaattgcg agcatggcat
     1021 tgcgtaaatt gggtcccaga aaagcggcga tggcgatggc gagcagcaga ggcggaaagg
     1081 agaggaatac atcggtaatg cgcatgatga cctcatccac aatgccgccc attccaccgg
     1141 cgatagctcc caggggcaca ccgatcagca gcgctaaacc gaccgcgatc agcgcagttt
     1201 gcagcgagat gcgggtgccg tagatcaccc gggagagcat atcgcgtccc aactcgtcgg
     1261 tgccgaacag gtatttcgct gagggcgcct ggagtttgtc taccggattc gtttcactga
     1321 tggcatgccc cgggtaggga gcaattatcg gagcgaagac agccaccagg atcaacagga
     1381 tgatgatggt cagtgaggca agctgcagct tattgcgggt aagcagaaaa aacgacaggc
     1441 gaaactcgcg caggctgggt tggagggtct ttctggtatc cattatccac tgcatgatga
     1501 ctgtctccta tgcccgcacg cgcggatcca tggcgatgat aatatcagcc accagattga
     1561 ggatcacgta tgaaaaggct gaaaatatgg tcacacccat gattgcaggg taatccagac
     1621 cgatgaccgc ggtggcaata taggaaccga tgcccggcca ggagaagatc gattccgtca
     1681 gaaatgtatt gaccagcgta tagccgatgg agagcgccac cacggtcgag gtagagccaa
     1741 ttgaattctt cagcgcgtat gaccagagca caatccgctc gggcaggccg taggagcggg
     1801 cggcagtaat gtaatcctca ttcaggatct ccagcagtgc cgagcgcgtc atccgcgcta
     1861 ccaggccgac aggatagagc ccgatggtga tgcccggtag aacgatatgc cagagagcat
     1921 cgaagaagat gcgaaaattt ccagtcacca gcgagtcgaa cataaggaag ccagtcagct
     1981 tcggtatctc catcgtgagc ttgatctcgg tggaaagctg cccgcccatt gggaagagat
     2041 gcagggtgcg gaagaataag agctgtaaaa tcaggcccac ccagaaagtg ggcagcgata
     2101 caacgccaac cgagaagagg cggctgatgt ggtcaaccag atggtctttc cgtttagccg
     2161 agatcacgcc cagggggatg ccgatgaaca cagccgcaac cgttgagatg aacaccagct
     2221 caatggttgc tggcagattt tcctgcagct cgagcataat cggccggcgg gtgcgcagcg
     2281 aagcgcccag gttgccctgc agcaggtcgc ccatatagcg gacaaactgc acatacagcg
     2341 gttggtccaa cccgagatcg atcgttgctt tagcaatctg ctcaggcgtg gcgcgcggac
     2401 ccacccactt ggctgccggg tctgatggaa tgacccgggt caggaagaag gtgatcaagg
     2461 ccacacctaa catcacgatg atgcccagga tcaaacgcct gagaatgaat tttttcattc
     2521 aggttcctac aaaccacccc tggttattat cctccatttt cgcctcgggc gggtgatatc
     2581 aatctgagag gcgaaaatgg aggggaaagg ggacctgcta ctgcaggtac atatcgtgga
     2641 agaacatcac cgtggggtaa gccgggttga agctaaatcc ctgcaggttg gccaccgtga
     2701 cccacacatc ctggcggtca tagatgaaca ggctggcagc gtcgtccaga agaattttct
     2761 gagcatcgac aaacatctgt tcagcctggg caggatcgtt ggcagtgact tcgttcgcct
     2821 ggtcgatcaa agtgtcaaac tcggggttat agtaatacga caagttgaag agtggttctg
     2881 cctcggtgtg gaaggtgctg tagaggaagg agtaggggct ggggagatcg ggccaccagt
     2941 acatcacgaa gatgtcctgg cgctgtgttg gatcagggtc ctttgccatt tcccactggc
     3001 tgtcccaggg catactgcgg atttcgaggt cgatattcag cttggcaagc tcggatttat
     3061 acagctcggc ggtgcttttc tcagtttcat tgccggcggt ataagtgagg agcagcttct
     3121 gccccgcagg tacaccggct tcggttaata gctgctgcgc tttctcgaaa tcatacttgt
     3181 actggggcag cgattcgctg taaccccaca taccgaaagg caccataccg actgcctggc
     3241 gggcataccc accaacagct gcagaaatca cctggtcgta agggaaagca taggccaggg
     3301 cctggcggac ggccttgtta tccaggggcg gtttttgggt attaaacatg ccgatcaggt
     3361 tctgccagga gggttcaaca tttactttaa cggcaggatt ttccttgagg gcattcacat
     3421 ccgaataggg gagctcgacg gcgatggtcg cttcaccagc ctcaatcatc tggcggcggg
     3481 tcgcagtttc agagatcttc ttgaatacca cctgtgagaa gaatttctca
//
LOCUS       DATMXK010000546         5590 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_131507, whole genome shotgun sequence.
ACCESSION   DATMXK010000546 DATMXK010000000
VERSION     DATMXK010000546.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5590)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5590)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5590
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_131507"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..281)
                     /locus_tag="VN363_09905"
     CDS             complement(<1..281)
                     /locus_tag="VN363_09905"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84872.1"
                     /translation="MDKKLQSVFLTLLAILVVFIALVYIGGNVMGIERLQTGENPVLR
                     FVFVIIGLLIAFGIYYSTRTNKIWEVGTREVVWMAIGAALYAVLSWLFNG"
     gene            complement(325..1965)
                     /locus_tag="VN363_09910"
     CDS             complement(325..1965)
                     /locus_tag="VN363_09910"
                     /inference="COORDINATES: protein motif:HMM:NF010167.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84873.1"
                     /translation="MDTSPLVIDDLSFRYRDRTGAAIQHISFAAQPGEILLIAGASGC
                     GKTTLIRAINGLIPRSYKGELSGQILLHGENPRDWSLARISQQVGTVLQDPERQILGT
                     KVLNEVAFGLENLGLERAEILERVHEALGLLNILHLKDRETFNLSGGEKQKVALAGVL
                     AMRPGILLLDEPLASLDPASAQDTLDTVRKLADQGMAVLIVEHRVEDVMRIKPERVLF
                     MADGEMRYLGDFAGLNEVVDYHQIKLPASLVMARAAADPVPEPLNILPSAQEHAQEHA
                     QAPAPSSNPLVQFEQVSFGYEKELTVLHGIDLDIRRGDVIAVLGPNGAGKTTFVKHAI
                     GLLKPKAGRVLVDGRDTRTASVAQIAATLGYVFQSPSHMLFAPTVHEELAFGPTNLKH
                     SKDQIEQEVKEALITVNLTDHEADPPLSLSFGQQKRVSIAAILAMRSRVLIMDEPTAG
                     QDYQNYMNFMDAILQLPGFEAILFITHDVDLAVIYANRVLLIAEGRLVADGAPQEALS
                     DLDRLRQCRLVPSSLLLANLERYPRSGQFLRAEALAHI"
     gene            complement(2158..5451)
                     /locus_tag="VN363_09915"
     CDS             complement(2158..5451)
                     /locus_tag="VN363_09915"
                     /inference="COORDINATES: protein motif:HMM:NF033681.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ExeM/NucH family extracellular endonuclease"
                     /protein_id="HWQ84874.1"
                     /translation="MSYQTRWFRILSGALILTLVLASAPLAGFSPHRAFAQTEPVFIN
                     EIHYDNTGTDAGEAIEIAGPAGTDLTGWSLVLYNGTGGVVYDTDPLSGTIPNLQNGFG
                     VLFFTYAVNGIQNGPPDGLALVAPGNAVVQFLSYEGTFTAVGGPADGLLSEDIGVFEN
                     GSEPLGLSLQLTGTGSLDGDFTWTAPDTSTFGAVNTGQTFEPVVVGPAEPVINEFSAS
                     TTGTDVEYVEIFGTASQDYSTYTLLEIEGDSGAASGTVDEVIPLGLMDSNGLGLISLA
                     ANALENGTITLLLVENFTGALNADLDTNNDGVFEITPWSSIADAVAVNDGGAGDLTYG
                     VPALGPNYDGLSSFAPGGASRIPDGFDSNAISDWVRNDFELAGIPGFAGTIILGEAYN
                     TPGAPNQIFVPPPEACGDPFTPIYTVQGNADASPLAGTEVAVEGVVVGDFQNNASPDN
                     GNLNGFHVQDATGDADPATSDGVFIYAPGGMDVAAGDAVRVRGPVSEFNGMTEITASQ
                     IWLCSTGSSIAATPLSLPVSSVGDFEAFEGMFVTFPQPLTISEYFNFDRFGEIVLTSQ
                     RHLTPTAQFEPGPDAIAAAQQFLLDRITLDDGRSTSNPDPAIHPNGNIFDLNNLFRGG
                     DLVQNVTGVLDYGFNLYRIQPTLGADYFPVNVRPAQPDEVGGYTQVASFNVLNYFTTL
                     TSAGPICGPNQDEACRGADTLEEFVRQRSKIVAAISQIDADVVGLLEIENHPADVPTA
                     DLVSGLNAVLGAGAYDYIATGAIGTDVIRTALIYKPGRVTPLGSFKVLDTSVDPRFLD
                     DYNRPVLAQTFQDNYTGEIFTVAVNHLKSKGSDCNDVGDPDTGDGSGNCNLTRTAAAE
                     ALVDWLAADPTGSGDADFLIIGDLNSYDKEAPIDALVAGGYTDLVFQFLGEEAYSYVF
                     DGQTGYLDHALASSDMVGHITGASIWHINADEADLIDYDMSFKLPAQDLLYAPDAYRS
                     SDHDPVIVGICEATPPQLVVDLSQTLLWPPRHQYVTIQASLTASDNADADPTITLVYV
                     TSNEPDEGLGDGDLPDDIVIIDDQTFRLRAERSGLGNDRVYTITYLVTDDCGNSQYVE
                     AYVTIPHNR"
BASE COUNT         1251 a         1558 c         1626 g         1155 t
ORIGIN      
        1 ccattgaaga gccaggatag gacggcgtat aaggcagcgc caatcgccat ccacaccact
       61 tcacgggtgc caacctccca gatcttattg gtgcgggtgc tgtaatagat cccaaaagcg
      121 atcagcaagc caatgatgac aaagacgaag cgcagcacgg ggttctcacc ggtctgcagc
      181 cgttcaatgc ccatcacatt cccgccgata taaacgaggg caatgaatac gaccaggatt
      241 gccagcaggg tcagaaaaac agattgcagt ttcttgtcca ttagatcctc cttaagcgaa
      301 tcactcaccg aatgcaggat tggattaaat atgcgccagg gcttcggcgc gcagaaattg
      361 acctgagcgg ggatagcgtt ccaggttggc caggagtaaa gaggatggca ccaggcggca
      421 ctgacgtaaa cggtccaggt cgctcaaggc ttcttgcggc gcgccatcgg caaccaggcg
      481 cccttctgca atgagcagga cacggttggc gtaaataacc gccagatcga catcgtgggt
      541 gatgaacagg atcgcctcga agccaggcag ctgcaaaatg gcatccataa aattcatata
      601 attctgataa tcctgcccgg cagtcggctc gtccataatc aatacccgcg agcgcatcgc
      661 cagaattgcg gcaatgctca cccgcttctg ctgtccaaag gacagggaga gaggtgggtc
      721 agcttcatga tcggtaaggt taacggtaat cagcgcctct ttcacctcct gttcgatttg
      781 atccttcgag tgtttaaggt tggttggtcc aaatgccagc tcctcgtgca cggttggcgc
      841 gaacagcata tggctggggc tttgaaagac ataccccagc gtcgccgcga tctgggccac
      901 gctggctgta cgcgtatcac gcccatcgac cagcactcgc ccagctttag gcttgagcaa
      961 gccgatggcg tgcttgacaa aagtcgtttt tcccgcccca ttcggcccca gcacagcgat
     1021 cacatccccg cgtcggatgt ccaggtcgat cccatgcagg acagtcaact ctttttcgta
     1081 gccaaagctg acctgctcaa attggaccag gggattggag gatggggcag gtgcctgggc
     1141 gtgctcttgg gcgtgctctt gagcgctggg aaggatgttg agcggctcgg gaaccgggtc
     1201 agcagcggcg cgcgccatca ccaggctggc tggcagcttg atctgatggt aatccaccac
     1261 ctcattgagc ccggcaaagt cgcccaggta gcgcatctcg ccatcggcca taaacaggac
     1321 ccgctctggt ttaatgcgca tgacatcttc gacacgatgc tctactatca agacggccat
     1381 cccctggtct gccagcttgc gcacggtatc cagcgtatcc tgggcactgg cgggatccag
     1441 gctcgccagc ggctcatcca ggagcaggat gcccgggcgc attgccagca ctcctgccag
     1501 ggcgaccttc tgcttctcgc cgccagacag attgaatgtc tcacggtcct tcaaatggag
     1561 gatattcagt aagccaaggg cttcgtggac tcgctccagg atttctgccc gttccagtcc
     1621 caggttttcc agtccaaagg cgacttcatt gagcactttt gtgcccagga tctggcgctc
     1681 ggggtcctgc agcacggtac ccacttgctg tgaaatgcgc gccaggctcc agtcacgggg
     1741 attttccccg tgaagaagaa tctgcccgct cagctcgcct ttataactgc gcgggatgag
     1801 tccattgatg gcccggatca gggtggtctt tccgcacccg cttgccccgg cgatcagcaa
     1861 gatctcacca ggctgggcag caaaggaaat atgttggatc gcggcaccgg tccggtcgcg
     1921 atagcgaaaa gaaagatcat caatgaccag cggagaagta tccataatct gattgcttaa
     1981 cccagggatg ggggaagggt tgctgtatta tagttgaaaa ggactggaat gtttacgagg
     2041 tgaaaaaagg cctgtcttaa tataaaagtc tctcgcattt cggcgggaga cttcctcgtt
     2101 caggtgagtc ctcgttcaga ccagcctgaa cgaggactcg taaacttaaa gggtcaatta
     2161 ccggttgtgc gggatggtga catacgcttc aacatactgg ctgttaccgc agtcatccgt
     2221 caccagatag gtgatggtat agacccggtc gttgcccaga ccggaccgct cggcacgcag
     2281 ccggaaggtc tggtcatcga tgatcacaat atcatccggc aggtcgccat cgcccagccc
     2341 ctcatcaggc tcgttggaag tcacataaac cagggtgatt gtcggatcgg catcggcatt
     2401 gtcgctggcg gtaagcgaag cctggatggt cacatactgg tgccggggcg gccagagcag
     2461 ggtctggctt agatcgacaa ccagctgggg cggagtggct tcgcagatgc cgacgatcac
     2521 cggatcgtgg tcggaagaac gataggcatc cggggcatac agcaaatcct gggcgggtaa
     2581 tttgaagctc atgtcatagt cgatcaagtc cgcctcatcg gcattgatgt gccagatgga
     2641 cgctccggtg atatgaccga ccatatccga gctcgccagg gcatgatcca ggtagccggt
     2701 ctggccgtca aagacatacg agtaggcttc ttcgccgagg aactggaaga ccagatcggt
     2761 gtagccaccg gcgacaagtg catcgatcgg agcttcctta tcgtaggaat tgagatcgcc
     2821 gatgatcagg aagtcggcgt cgccgctgcc ggtaggatcc gcagccagcc agtcaaccag
     2881 ggcttcagcg gcggcggtgc gggtgaggtt gcagttgccc gagccatcgc cggtatccgg
     2941 gtcaccgaca tcattacagt cagagccctt ggatttcaga tggttcacgg cgacggtgaa
     3001 gatctcaccg gtgtaattat cctggaaggt ctgggccaga accggacgat tgtaatcatc
     3061 caaaaagcgc gggtcaacgg aggtatccag gaccttgaaa ctacccagcg gggttactcg
     3121 gccgggttta tagatgaggg cggtgcggat gacgtcggtg ccaattgcgc cggtagcgat
     3181 gtaatcatag gcgccagctc cgagcacagc gttcagcccg ctgaccagat cagcagtggg
     3241 gacatccgca gggtgattct cgatctccag cagtccaacg acatcggcgt cgatctggct
     3301 gatggcggcg actatcttgg atcgctggcg gacgaattcc tccagggtat ccgcgccgcg
     3361 gcaggcctca tcctggtttg gaccacagat ggggccagcg ctggtaaggg tcgtgaagta
     3421 attgagtaca ttaaagctcg cgacctgggt gtatccaccc acttcatctg gctgcgccgg
     3481 gcgcacattg accgggaagt aatcggctcc cagggtaggc tggatgcgat acaggttgaa
     3541 gccgtagtcc agcacgccgg tcacattttg taccaggtca ccgccgcgga acaaattatt
     3601 cagatcaaag atatttccat tggggtggat ggcgggatca gggttcgagg tggaccgccc
     3661 atcgtcgagg gtgatgcgat cgagcaggaa ctgctgagca gccgcgatgg catccggccc
     3721 cggctcaaat tgggcggtag gggtcaggtg gcgctgggag gtcaggacga tctccccaaa
     3781 gcggtcgaag ttgaagtact cagagatggt gagcggctga gggaaagtca cgaacatccc
     3841 ctcaaaggcc tcaaagtcgc ccacgctgct caccggtaag gagagaggcg tggcggcgat
     3901 gctgctgccg gtcgagcata accagatctg gctggcggta atttcggtca tcccgttgaa
     3961 ttcggagacc gggccgcgca cgcgcaccgc gtcgccggct gccacatcca ttcctcccgg
     4021 agcgtagatg aaaacgccgt ccgatgtcgc cgggtcagca tcacccgtgg catcctgtac
     4081 atggaaacca ttcaaattgc cgttgtcagg gctggcattg ttctggaaat cgcccaccac
     4141 cacgccttcg acagcaacct cagtgcctgc cagagggctg gcatccgcat taccctgtac
     4201 ggtgtagatc ggggtgaaag gatcgccgca ggcttcgggc ggtggtacaa aaatctggtt
     4261 cggagcgccg ggggtattgt aggcttcgcc cagaataatt gtgccggcga atcccgggat
     4321 acccgccagt tcgaagtcgt tgcgcaccca atcactgatc gcgttgctgt caaagccgtc
     4381 cgggatgcgg ctcgcgccac ccggggcaaa gctgcttaaa ccatcgtaat ttggtccaag
     4441 cgctggtaca ccgtaagtga gatcacctgc acccccgtcg tttaccgcaa ctgcgtcggc
     4501 tattgagctc cagggcgtta tctcaaagac gccatcattg ttcgtgtcca gatcggcatt
     4561 caaagctccg gtaaaattct cgaccagtaa caaagtgatg gtaccatttt cgagagcatt
     4621 cgcagctaac gaaatcaggc cgaggccgtt ggaatccatt aaccctaacg gaattacctc
     4681 gtccaccgtg cccgaggctg caccactgtc cccttcgatt tcgagaaggg tgtaggtcga
     4741 gtaatcctgg gaggctgtcc caaaaatctc tacatattcg acatccgtgc cggtggtgct
     4801 ggcggagaac tcgttaatta ctggctcagc cgggccgacg accacaggct caaaggtctg
     4861 cccggtattg acagcgccaa atgtgctggt gtccggagcc gtccaggtaa agtccccatc
     4921 aagactgccg gtccctgtga gttggagcga aagacccagt ggctcgctgc cgttttcgaa
     4981 gacaccgata tcctcactga gcaatccatc cgccggcccg cccactgcag taaaggtacc
     5041 ctcatagctc aggaattgaa cgactgcatt ccctggagcg accagtgcca ggccatccgg
     5101 gggtccattc tggatgccgt tgaccgcata ggtgaaaaac agcacgccga acccgttctg
     5161 taaattcggg atcgtgccgc ttaacggatc tgtgtcataa accacgcccc ctgtaccgtt
     5221 gtagagcacc agcgaccaac ccgtgagatc tgttccggca ggtccggcga tctcgatcgc
     5281 ctcgccggcg tcggtccccg tgttgtcata atgaatttca ttgataaaga ctggttcagt
     5341 ttgggcgaag gctcgatgcg gggagaaacc cgccagtgga gcacttgcca ggaccagagt
     5401 cagaatgagc gctcctgaga gaattcggaa ccaacgagtc tgataagaca tgatcaatct
     5461 cctattttcg gtgggttaac ttatcttgca agctggatgg aacgatcgat ccgcatacat
     5521 gtttggagaa gacaaacaag atcaggccag ccggcctcct tcctgggcga tggatcgatc
     5581 tgattattac
//
LOCUS       DATMXK010000547         4939 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_94028, whole genome shotgun sequence.
ACCESSION   DATMXK010000547 DATMXK010000000
VERSION     DATMXK010000547.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4939)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4939)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4939
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_94028"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VN363_09920"
     CDS             <1..366
                     /locus_tag="VN363_09920"
                     /inference="COORDINATES: protein motif:HMM:NF023478.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF3536 domain-containing protein"
                     /protein_id="HWQ84875.1"
                     /translation="ILGEMTAGQLIAEMAGRALTSEQIQRTHLLLEAQRERQRMFTSC
                     GWFFEDFDRIEPRNNVAYAAQAVLLTLQATGIDLSGMALRDLALVRSPRSGLTAERVF
                     LRHMERACSTAGERIGFAD"
     gene            complement(543..4079)
                     /locus_tag="VN363_09925"
     CDS             complement(543..4079)
                     /locus_tag="VN363_09925"
                     /inference="COORDINATES: protein motif:HMM:NF012356.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_process: GO:0005975 - carbohydrate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha-amylase family glycosyl hydrolase"
                     /protein_id="HWQ84876.1"
                     /translation="MPTLFPYRMEFHIARLVRDKYQFEEALFSLSGNVIFANFHAARL
                     FAQKMNARRDLIHFPEQAMQAGQINAMGMIDEIMHMVVSMYHKQRSPEAIADALAFLK
                     ERIGDEEVEQTLLRFVEQFPPLLVYQRQATPREYLDGESINADGKQISNQELVLEELL
                     MLWLANANPAFAPFLELFDDQDLKKTTAYPTMISGLKEFFDTQPTFGPDGQNLIDMLR
                     SPAIQIPYSLEGQLEYIRTRWLEILGSYLYRLLSSLDLIKEENKIIFPGGGPGPIRVY
                     DFRGQEGEAENFSPDKDWMPNLILIAKNSYVWLDQLSKKYKYAITRLDEIPDEELDNL
                     SCWGFTGLWLIGLWERSQASQRIKIMRGNPDAVASAYSLYSYDIAWDLGGEDAYRNLR
                     DRAWKRGIRLSSDMVPNHMGIDSRWVIEHPDWFVSLPYSPFPSYNFGGENLSGQDGVG
                     IYLEDHYFDNTDAAVVFKRVDFNSGDTRFIYHGNDGTSMPWNDTAQLNYLLPQVREAV
                     IQTILHVARKFPVIRFDAAMTLAKRHYQRLWFPEPGSGGDIASRSDYGMTKEQFNAAF
                     PNEFWREVVDRVAQEVPDTLLLAEAFWLMEGYFVRTLGMHRVYNSAFMNMLRDEKNAE
                     YRQAIKNTLEFEPEILKRYVNFMNNPDERTAVEQYGKGDKYFGICIVMATLPGLPMFG
                     HGQIEGYTEKYGMEFRRAYWDEHPDGYLVERHEREVFPLLRKRYLFAGVENFLLYDFY
                     TPQGYVDENVYAYSNRVGEQRGLVVYQNKFGDTRGWIRTSAAYLDKGSRGLAQKSIGE
                     GLALPNDPDLYVVFRDPIRGLEYIRSSTEIHSNGLFVELDAYQYRVYLDFRVIQDNAW
                     KHYAQLTAYLNDGGVPNIEEALQQIFLQPVHRAYRELINAGSLNWLLAHHSRAEAKFD
                     LTRLQAFAEVETKLGALLGEIRRYTNAPGDVEALASEILSDLKKLLALPGLEKSSAAE
                     LQPAADYLLKGRATSPALARFELPVWGALLTWTITRKLGKLLDASPEGYPEQSRSWID
                     EWMLGKFIRQALQDQGADEGRAEHLTGLVRLLTGQQNWYDPQTPLEGRAFKSLQSLLR
                     DGLVQRYIGENRHQDVLWFNQESFEELLWWLFTIAVVDLAPAVPEPGSKTSPMNKAIT
                     ELFAVVEALAKAEAESGYQVEKLLAGVAPKAD"
     gene            complement(4268..>4939)
                     /locus_tag="VN363_09930"
     CDS             complement(4268..>4939)
                     /locus_tag="VN363_09930"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84877.1"
                     /translation="PTQIDLVNLDGSNRRPAVLTFEMVLTYSEYMISPPVTWAADSSM
                     VWAAIPPHDALGAPPQITTVWQIPVEGSPAVKTAEFQAAPLVGFELPVSPDTQHLVYL
                     AETGEQPDIQYELHLAALDGSSNQILHTAPFMDFLSWSPDGQRFAVSVGNEQALYLGE
                     AAGSFAPAPGSPAGVSDLDWMDEAQFVYVQTGAGGASLVHGAVTGTPQVMDSLNGGFI
                     PYSVR"
BASE COUNT          933 a         1452 c         1494 g         1060 t
ORIGIN      
        1 atcctggggg agatgaccgc cgggcagttg attgccgaaa tggcgggccg cgccctgacc
       61 agcgagcaga tccagcgcac ccacctgctg ctggaggcgc agcgcgagcg ccagcgcatg
      121 ttcacttcgt gcggctggtt cttcgaggat ttcgaccgca tcgagccgcg caacaacgtg
      181 gcctacgctg cccaggcggt cctgctcacc ctgcaggcga cggggatcga cctgagtgga
      241 atggcactgc gcgatctggc cctggtgcgc agcccgcgct ccggactcac tgccgagcgg
      301 gtcttcctgc gccacatgga gcgcgcctgc tccaccgccg gtgaacggat cggctttgca
      361 gactgatcgt ccccctgttg aaatttcagc tccataaacc agacccctgg ggtttctgaa
      421 accccagggg tctttttgga tagaaaaccg gataattatc cctatttcaa atcctgttca
      481 tcctgtccat ccatgtaaat tcctggtttt ctctgcgccc cctgcgtccc tgcgagagaa
      541 tcctaatccg cctttggagc cacccccgcc agcagtttct ccacctggta gcccgattct
      601 gcttctgctt tcgccagcgc ctcgaccacc gcgaacagct cggtgatcgc tttgttcatg
      661 ggcgaggttt tactgcccgg ctccgggacg gcaggcgcca ggtctaccac ggcgatcgtg
      721 aacagccacc acagcagctc ttcaaagctc tcctgattga accacaacac atcctggtgg
      781 cggttttcgc cgatgtagcg ctgcaccagt ccatcccgca gcaggctttg caggctcttg
      841 aaggcgcgtc cctccagcgg ggtttgcggg tcgtaccagt tctgctgccc ggtgagcagg
      901 cgcaccaggc cggtcaggtg ctcggcgcgg ccttcatcgg caccctgatc ttgcagcgcc
      961 tggcggatga acttgcccag catccattca tcgatccagg agcggctctg ttcgggatag
     1021 ccttccggag aagcatccag cagcttgccc agtttgcgcg tgattgtcca ggtcagcaaa
     1081 gcgccccaca ccggcagctc aaagcgtgcc agcgcagggc tggtggcgcg gcctttcagc
     1141 agatagtctg ctgccggctg cagctcagcg gcgctggatt tttccaggcc gggcagtgcc
     1201 agcagcttct tcaggtcgct caggatctcg ctcgccagcg cttccacgtc tcccggcgca
     1261 ttggtgtagc ggcggatctc gcccaacagc gcacccagct tggtctccac ctccgcgaat
     1321 gcctggaggc gggtgaggtc aaattttgcc tccgccctgc tgtgatgcgc cagcagccag
     1381 ttgagcgagc cggcgttgat cagctcgcgg taagcccggt ggacgggctg caggaagatc
     1441 tgctgcaggg cttcttcgat attgggtacc ccgccgtcgt tcaggtaggc ggtcagctgg
     1501 gcgtaatgct tccaggcgtt atcctggatc acccggaaat cgaggtacac ccggtactgg
     1561 taagcgtcca gctctacgaa caggccattg ctgtggattt cagtgctgct gcggatatac
     1621 tccaggccgc gaatcgggtc gcggaagacc acgtacaggt cgggatcgtt gggcagtgcc
     1681 agtccttcgc caatcgattt ctgggccagg ccgcggctgc ctttatccag gtaagccgcc
     1741 gaggtgcgga tccagccgcg ggtatcgccg aacttgtttt ggtacaccac cagcccgcgc
     1801 tgctcgccca cccggttgga gtaggcgtac acgttctcgt cgacataccc ttggggggtg
     1861 tagaaatcat acagcaggaa gttttctacc ccggcaaaca ggtagcgctt gcgcagcagt
     1921 gggaagactt cccgttcgtg gcgctccacc aggtagccgt ccgggtgctc atcccaatag
     1981 gcgcggcgga attccatgcc gtatttctcg gtatagccct cgatctgccc gtgcccaaac
     2041 atgggcagcc cgggtagagt agccattacg atgcagatgc cgaagtattt atcgcccttg
     2101 ccgtactgct ctactgccgt gcgctcgtcg gggttgttca tgaagttgac gtagcgcttg
     2161 aggatctcgg gctcgaactc cagggtgttc ttgatcgcct ggcggtattc ggcgttcttc
     2221 tcatcgcgca gcatgttcat aaaggcactg ttgtacaccc ggtgcatgcc cagggtacgc
     2281 acgaaataac cttccatcag ccagaaggct tccgccagca gcagggtgtc gggcacttcc
     2341 tgcgccaccc ggtccaccac ctcgcgccag aattcgttgg ggaaggcggc gttgaactgc
     2401 tctttagtca tgccgtaatc ggagcgtgag gcgatatccc cgccgctgcc cggctctggg
     2461 aaccacaggc gctggtagtg gcgcttcgcc agggtcatgg ccgcgtcgaa gcggattacc
     2521 gggaatttac gcgccacgtg caggatggtc tggatcacgg cttcgcgcac ctggggcagc
     2581 aggtagttga gctgggcggt gtcgttccag ggcatggagg tgccgtcgtt gccgtggtag
     2641 atgaagcgcg tgtcgccgct gttgaagtcc acccgcttga aaaccaccgc cgcatcggtg
     2701 ttgtcgaagt agtgatcttc caggtagatg cccacgccgt cctgtcccga gaggttctcg
     2761 ccgccaaagt tgtaggatgg gaaggggctg tagggcaggc tcacaaacca atccgggtgt
     2821 tcgatcaccc agcgtgagtc gatccccata tggttgggga ccatatctga agacaggcgg
     2881 atgccgcgct tccaggcccg gtcgcgcagg ttgcggtagg catcctcgcc gcccaggtcc
     2941 caggcgatat cgtaagagta gagcgagtaa gccgaggcga ctgcatccgg gttgccgcgc
     3001 atgatcttaa tccgctggga ggcctgactg cgctcccaca ggccgatcag ccacagcccg
     3061 gtgaaacccc agcagctcag attgtccagc tcctcgtcgg ggatctcgtc cagacgggtg
     3121 atagcgtatt tatatttttt agagagctgg tctaaccaga catacgagtt tttggcgatc
     3181 aggatcagat tgggcatcca atctttatcc gggctgaagt tttcggcttc gccttcctgg
     3241 ccgcggaaat cgtacacccg gatggggccc ggcccgccgc ccgggaagat gattttattt
     3301 tcttctttga tcaaatccag gctgctcagc aggcgataca gatagctccc cagaatctcc
     3361 agccagcgag tgcggatata ttcgagctgg ccttccaacg aataagggat ctggattgcc
     3421 gggctgcgca gcatatcgat caggttctgc ccatccgggc cgaaggtggg ttgggtatca
     3481 aaaaactcct tcaatccgct gatcattgtg gggtaggcgg tcgttttttt caggtcctgg
     3541 tcatcgaaca gctccaggaa gggggcgaaa gccgggttgg cgtttgccag ccacagcatc
     3601 agcagctctt ccaggaccag ctcctggttt gagatttgtt taccgtctgc atttatggat
     3661 tcgccgtcca ggtattcccg gggtgttgcc tggcgctggt agacgagcag gggagggaac
     3721 tgctcgacga agcgcagcag agtttgctcg acttcctcat ccccaatgcg ctctttaagg
     3781 aatgccaggg catctgcgat tgcttccggg ctgcgctgct tatggtacat actcacgacc
     3841 atatgcatga tctcatcgat catgcccatg gcgttgatct gcccggcctg catggcctgt
     3901 tccggaaagt ggatcaggtc gcggcgggca ttcattttct gagcgaataa gcgggcagcg
     3961 tggaaattgg caaagattac attaccgctc aggctgaaca gcgcttcttc gaactggtat
     4021 ttatcccgca caaggcgcgc gatatggaat tccatgcgat acggaaacaa ggttggcata
     4081 actcctccca ccacgaacct ctggatattt gtcaggcgca gcccgagcgc ccagggaaaa
     4141 tcggtaattt aaccaatatt atacctgtac aggatgcacc ctgtcagtgc agtccacaga
     4201 ttaccggagg cggtgataaa aggggagggt ccagtgtgtc gctcacactg gaccctgaaa
     4261 cgagaattta gcggactgag taggggataa agcccccatt gagggagtcc atcacctgtg
     4321 gtgtgccagt gaccgcgccg tgaacgaggc ttgcgccgcc cgcaccggtc tgaacataca
     4381 caaactgagc ttcatccatc cagtccaggt cggagactcc tgccggtgag ccgggagccg
     4441 gagcaaagct gccggcggct tcgccaaggt acagggcttg ctcattgcct acgctgacgg
     4501 cgaaacgctg cccgtctgga gaccagctga gaaaatccat gaagggtgcg gtgtggagga
     4561 tctggtttga gctgccatcc agggctgcca gatgcagctc gtactggata tcgggctgtt
     4621 cgccggtttc tgccaggtat accaggtgct gtgtatccgg ggagaccggc agctcaaatc
     4681 cgaccagggg cgcggcctgg aattcagcgg ttttaactgc cggtgagcct tctacaggga
     4741 tctgccagac tgtggtgatt tgaggagggg cacccagggc gtcgtgggga gggatggcag
     4801 cccaaaccat gctggaatcc gctgcccagg tcaccggcgg gctgatcata tattcgctgt
     4861 aggtgagcac catttcgaaa gttaaaactg caggacgccg gttgctgccg tccaggttga
     4921 ccaggtcgat ctgggtggg
//
LOCUS       DATMXK010000548         3462 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_73091, whole genome shotgun sequence.
ACCESSION   DATMXK010000548 DATMXK010000000
VERSION     DATMXK010000548.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3462)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3462)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3462
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_73091"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            157..1056
                     /locus_tag="VN363_09935"
     CDS             157..1056
                     /locus_tag="VN363_09935"
                     /EC_number="2.1.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024880.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="class I SAM-dependent methyltransferase"
                     /protein_id="HWQ84878.1"
                     /translation="MPIIVEHCPLCGDSRSTQFDQRRFRDQVVTNRLCLRCGFVFQSP
                     RMTDIELADFYQAEYRRLYQGSAGPNAKDLAVQRGRAQVLVEFTRRVIKPGIRHLDIG
                     CSSGLLLQEFASAFTSTPTGIEPGDAYRSYARAQGLPVYASLDALLADQPEQFNLISM
                     AHVLEHIAEPVAYLSNLREKLLAPDGWLLLEVPNLYAHDSFEIAHLLAFSPHTLAETV
                     RQAGFQISIIRQTGLPRSSWIPLYIQLLAQPDPAAGKVAVKQEKLVGLKRQLGLVQRR
                     LLTRLFPQRAWQSPDSSQVEGRA"
     gene            1053..1955
                     /locus_tag="VN363_09940"
     CDS             1053..1955
                     /locus_tag="VN363_09940"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84879.1"
                     /translation="MSFLTIFSAPKPFTNPHIATIQRNAIESWLHLAEDVDVVLVGEE
                     AGLAEAAAKAKVPLLSQVARTASGTPLVSSIFNLARQHSQSPLLGYVNADMLFLPDLI
                     QAARTVASQSEKFLIIGQRWDLDQPTLLDFSNGWENRLRADIRQRGRLHTPSGSDYFI
                     FPRALFAEMPDFAIGRAGWDNWMIYFARHQGWPVVDGTPSILAIHQDHDYSHLPGGQP
                     HYNHAESRQNEILAGGSNKLFMVLDSDRQLRNGQIRSPKPTLLRVLRQAEVGLNPPDG
                     RPNQLKKSLARQARRLRRRISGTL"
     gene            1962..3038
                     /locus_tag="VN363_09945"
     CDS             1962..3038
                     /locus_tag="VN363_09945"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWQ84880.1"
                     /translation="MRLGQNPAKSIAQVPQPAPVTVAIVTYIPFLSGYYAESLEVLKV
                     CLESLVQNTPQPFDLLVFDNASCPEVRSYLQNEHDAGRIQYLILAGQNFGKGGAWNFI
                     FQGAPGEIIAYADSDIQFSPGWLESSLEILQAFPKAGMVSAHPLRTPAALYSSTLAWA
                     QLASQAGLASQSAPASQAGQAGHTEQAGHNSQNEHTPRVGQSDPFDPDDEVQIEQGSF
                     IPWEVYREHVLSLGTSEEQAREWYESRQDTRLTRQGVQALIGAAHFQFTVRKSIIQAF
                     LPFKMDRPMGQVRTLDDQMNAAGWLRLATCEPLVRHLGNTLPVEPKTGQQLRTDHRRL
                     WDTPVIRRVLLRVYDVIFRLYYGR"
     gene            3054..>3462
                     /locus_tag="VN363_09950"
     CDS             3054..>3462
                     /locus_tag="VN363_09950"
                     /EC_number="2.4.-.-"
                     /inference="COORDINATES: protein motif:HMM:NF012745.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="glycosyltransferase family A protein"
                     /protein_id="HWQ84881.1"
                     /translation="MRTGRNPAKSGLEAYTPKELAVALILYIPSQDGYFANALEIFKM
                     QLASIKAATSQPYDLLVFDNGSCASVVQTLQELYQAGEITWLVLSRHNIGKAGAWNWI
                     FASLPNPLIGYADADVLFRPGWFEASQEILKNIP"
BASE COUNT          761 a         1012 c          917 g          772 t
ORIGIN      
        1 gtgggaagat gcctgtacca ccctcagcag tattcgacag gaatatcccc tgacgcacgt
       61 tatattcttt aagaaaaaag cccagttatc ctgcgagttc gttagcgaag agtttcgcaa
      121 cgcccaatac agcgtgtata agatcaaccc atagccatgc caatcatcgt tgagcactgc
      181 cccttatgtg gtgatagccg ctccacccag ttcgaccagc gccggtttcg cgatcaggtg
      241 gtaactaacc gtctgtgcct gagatgcggt tttgttttcc agtccccgcg catgacagat
      301 attgagctgg ctgactttta ccaggccgag tatcgcaggc tgtaccaggg cagcgccggc
      361 ccgaacgcca aagatctggc agtccagcgg gggcgcgctc aggtgctggt cgaatttaca
      421 cgccgggtaa tcaaaccggg gattcgccac ctggatatcg gctgctcgag cggtttgctg
      481 ttacaggaat ttgccagtgc ttttacctcc actccgaccg gtattgagcc tggtgatgct
      541 tatcgaagct atgcccgggc acagggctta cccgtatatg cttccctgga tgccttactg
      601 gctgaccaac ccgagcaatt caacctgatc agcatggcgc atgttctcga gcacatcgct
      661 gagccggtcg cctacctgag taacctgcgc gagaagttac tcgccccaga cggatggctg
      721 ttgttggaag tgccgaacct atacgctcat gacagttttg aaatcgcgca cctgctggca
      781 ttcagccccc ataccctggc agaaactgtc cgccaggcgg gcttccagat cagtattatc
      841 cgccagaccg ggctgccccg ctcttcgtgg attcccctgt atatccagct cctggctcag
      901 cctgatccgg ccgcgggcaa ggttgcggta aagcaagaga agctggtcgg attaaaacgc
      961 cagctgggat tggtccaaag gagactgcta acccggctgt ttccgcagcg tgcctggcaa
     1021 agcccagatt cctcccaagt ggagggcagg gcgtgagttt cctgaccata ttttcggcac
     1081 ccaagccgtt caccaacccg catatcgcta ccatccagcg caacgcgatc gagtcctggc
     1141 tgcatctcgc tgaagatgtg gatgtcgtct tggttggtga agaagcaggt cttgccgaag
     1201 ctgcagccaa agccaaggtc ccccttctct cccaggtcgc gcgcacagcc agcggtacgc
     1261 cgctggtcag ctccatattc aacctggccc gccaacacag tcaaagccct ttgctggggt
     1321 atgttaacgc cgatatgctg tttttacctg atttaatcca ggcagccaga accgttgcca
     1381 gccaatcgga aaagttcttg atcataggac agcgctggga tttagaccag cccaccttgc
     1441 tcgatttctc aaatgggtgg gaaaatcgct tacgagcgga tatccgccag cgcggccggt
     1501 tacacacgcc ttcaggcagc gattatttca ttttcccacg cgccctgttt gcagaaatgc
     1561 cggatttcgc tattggccgg gctggctggg ataactggat gatttatttc gcccgccacc
     1621 agggctggcc ggtagtggat gggactccct ctattctggc aatccatcaa gatcatgatt
     1681 acagccacct gccgggcgga cagccccatt acaaccatgc cgaatcgcgc cagaatgaaa
     1741 tcctggcggg cggatcaaat aagctgttta tggtgctgga cagcgaccgg cagctgagga
     1801 atggacagat ccgaagtcct aagccaaccc tgctgcgagt tctgcgccag gctgaagtcg
     1861 gcctgaatcc accggatggc aggccgaacc agctcaaaaa aagcctggcc cgccaggccc
     1921 gccgcttgcg ccgccggatc agcggtactt tataaactca aatgcgcctc ggacaaaatc
     1981 cagcaaaatc catcgcgcaa gttccccagc cggctccggt cacagtcgcc atcgtgacct
     2041 atatcccgtt cttaagcggt tattacgccg aatcgttaga agtattgaaa gtttgcctgg
     2101 agagcctggt tcagaacaca cctcagccct ttgatttatt ggttttcgat aatgccagct
     2161 gcccggaagt ccgcagttat ctccaaaacg agcatgatgc tggacggatc caatacctga
     2221 tccttgccgg acagaatttt ggaaaaggcg gcgcctggaa ctttatcttc cagggtgcac
     2281 cgggcgagat tatcgcctat gccgacagcg atatccaatt ctcacccggc tggctggaaa
     2341 gcagcctgga aatcctgcag gcttttccca aagctggcat ggtcagtgcc cacccactgc
     2401 gcaccccggc tgccctgtat tcgagcacgc tggcatgggc gcagcttgcg tctcaagctg
     2461 ggcttgcttc ccaatctgcg cctgcttcgc aagctgggca ggctgggcat accgagcaag
     2521 ccgggcataa ttcgcaaaac gagcatactc cccgggttgg acagagtgat ccgtttgatc
     2581 cggatgatga agttcagatt gagcagggca gcttcatccc ctgggaggtc tatcgagaac
     2641 atgtgctcag ccttggaacc tccgaagagc aggcccgtga atggtatgaa tcgcgtcagg
     2701 atactcgcct cacccgccag ggagtccagg cgctgatcgg cgcagcccac tttcaattta
     2761 cagtccgtaa atcgatcatt caggcattcc tgcccttcaa aatggaccgg cccatgggtc
     2821 aggtgcgcac cctggatgat caaatgaacg ccgccggctg gctgcggctc gctacctgcg
     2881 agccattggt ccgccattta ggaaatacac tgccggttga gccaaaaaca gggcagcagc
     2941 tgcgcacaga ccaccgccga ctctgggata cccccgtcat ccggcgggtc ctgctgcggg
     3001 tttatgatgt gatcttccgc ctttattatg gtcgttaaaa gtattccaac ccgatgagaa
     3061 ctggacgtaa cccggctaaa tctggactgg aagcttacac tcccaaagag ctggctgttg
     3121 ctttaatttt gtatatacca tctcaagatg gttatttcgc caatgcctta gaaatcttca
     3181 agatgcagct ggcgagcatc aaggcagcca cctcccagcc gtatgatttg ctggtattcg
     3241 ataatggcag ctgcgcctcg gtggttcaga ccttgcagga gctttatcag gcaggcgaga
     3301 tcacctggct ggtattgtcg cggcataata tcggcaaagc cggcgcctgg aactggattt
     3361 ttgcttcact gcccaatcca ctgatcgggt atgccgacgc cgatgtgctc ttccgccccg
     3421 gctggtttga agccagccag gaaatcttga agaatattcc cc
//
LOCUS       DATMXK010000549         2049 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_85236, whole genome shotgun sequence.
ACCESSION   DATMXK010000549 DATMXK010000000
VERSION     DATMXK010000549.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2049)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2049)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2049
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85236"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..308)
                     /locus_tag="VN363_09955"
     CDS             complement(<1..308)
                     /locus_tag="VN363_09955"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_process: GO:0015031 - protein transport [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="twin-arginine translocase TatA/TatE family
                     subunit"
                     /protein_id="HWQ84882.1"
                     /translation="MDILGIGPLELVFVLLIALIVLGPNDMVKAGRTLGRFLRKLVTS
                     PGWQMVQQTSKDLRYLPNKLMREAGLEELKEKMPTQAEISKGIGLDDLKQEVREMDRE
                     L"
     gene            complement(320..769)
                     /gene="nusB"
                     /locus_tag="VN363_09960"
     CDS             complement(320..769)
                     /gene="nusB"
                     /locus_tag="VN363_09960"
                     /inference="COORDINATES: protein motif:HMM:TIGR01951.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="transcription antitermination factor NusB"
                     /protein_id="HWQ84883.1"
                     /translation="MKPRTRARSVALQALYEIDIAGHPPGEVLQERIEEADLDEGLAK
                     FCRAIVLGILPIVHELDGFIGEHAPEWPLEQVAIIDRNIIRIALWEFAIYTKTPIKVA
                     INEAVELAKSYGSDSTPRFVNGVLGSLAARQNDIKQALSRFSTGPEE"
     gene            complement(776..1177)
                     /locus_tag="VN363_09965"
     CDS             complement(776..1177)
                     /locus_tag="VN363_09965"
                     /inference="COORDINATES: protein motif:HMM:NF015721.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Asp23/Gls24 family envelope stress response
                     protein"
                     /protein_id="HWQ84884.1"
                     /translation="MSKTELKESPETSEAGEGYERNGKTTIAPEVLLTIARLTVLAVP
                     GVSRMSQTPGGVNRLFKRGYGEGVHLDIRDDDHVYADLYVILHSDVNIRDISRRIQLA
                     VTRAITDMVGMPVGRVNVHIDDIDFPVESEA"
     gene            complement(1199..>2049)
                     /gene="fabG"
                     /locus_tag="VN363_09970"
     CDS             complement(1199..>2049)
                     /gene="fabG"
                     /locus_tag="VN363_09970"
                     /EC_number="1.1.1.100"
                     /inference="COORDINATES: protein motif:HMM:NF005559.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004316 - 3-oxoacyl-[acyl-carrier-protein]
                     reductase (NADPH) activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="3-oxoacyl-[acyl-carrier-protein] reductase"
                     /protein_id="HWQ84885.1"
                     /translation="ILRGIISFANCQPIPNDFRQSWRVWLISIEEHSGMDFNGRVALV
                     TGGSRGIGRAVVEALAAGGAQVALASRSLEQSQAAAQEIAAQCSTRVLGYAVDVADPA
                     QAKALVEQVVADFGRIDILVNNAGTTRDNLIMRMNEADWDIVLDTNLKGTFNFCKAAI
                     RSMLKQRYGRIVNLSSVSGLAGQAGQANYSASKAGIIGLSKALAREVASRGITVNVVA
                     PGFVPTALTADLPENLKTQSLSTIPLGRWGEPDEIAVAVAFLASDKASYITGQVLSVD
                     GGMAMM"
BASE COUNT          450 a          593 c          515 g          491 t
ORIGIN      
        1 agctcacgat ccatctcgcg tacctcttgc tttaaatcat ccagaccgat tcctttggaa
       61 atctccgcct gggtgggcat tttttctttt aattcttcca acccggcttc tcgcatcagc
      121 ttattgggta aatatcgcag gtcttttgaa gtctgctgta ccatctgcca gccgggggag
      181 gtgaccagct tacgcaggaa tcgtccgagg gtcctgccag ccttgaccat atcatttggg
      241 ccaagcacga tcagggcaat taataagaca aacactaact cgagtggtcc aataccgaga
      301 atatccattt ttactgcttt tattcctccg ggccggtaga aaaacggctc agggcttgct
      361 taatatcatt ttgacgggct gccaggctgc ctaaaacccc attgacgaag cgcggggtgc
      421 tgtccgagcc ataggatttt gccagctcca ccgcctcatt gatcgcaact ttgatcggtg
      481 ttttcgtgta aatcgcaaat tcccacaatg caatccggat tatattgcgg tcgatgatcg
      541 ccacctgctc gagaggccat tccggtgcgt gctcgccgat gaagccatcc agctcgtgca
      601 caatcggtag gatccccagg acgatggcgc ggcaaaactt tgccaggcct tcatccagat
      661 cggcttcctc aatccgctcc tggagaactt ctccaggggg gtgaccggcg atatcaattt
      721 cgtaaagtgc ttgcagggct acactgcgtg ccctggtacg gggtttcatc aaagatcagg
      781 cctcagactc cacaggaaaa tcgatatcat caatatgaac gttcacccgc ccgacaggca
      841 tgcccaccat atcggtgatt gcccgggtaa ctgcgagctg aatccggcgg ctgatgtcgc
      901 ggatattgac gtctgaatgc aagattacat acagatcagc atagacgtgg tcgtcatcgc
      961 gaatatccag gtgcacgccc tcaccatagc cgcgcttaaa aagacgattg accccaccgg
     1021 gagtctggct catccggctg acgcctggaa cggcgaggac agtcaggcgg gcaatggtca
     1081 acaggacttc gggcgcgatc gttgttttac cgttgcgctc atacccctca ccggcttcgc
     1141 tggtctcagg agattctttt aattcggttt tgctcatttt tcctccatcc cttcaggctt
     1201 acatcatcgc catgccgcca tctacactta acacctgacc ggtaatgtag ctggctttat
     1261 ctgaggccag gaaagcgact gccacggcaa tttcgtccgg ctctccccag cgacccagcg
     1321 gaattgtgct cagactctga gttttaagat tttccggcag gtctgccgtc agcgcagtgg
     1381 gcacaaagcc gggtgcgacc acattgacgg tgattccccg cgaggcgact tcgcgggcca
     1441 gtgcttttga aagtccgatg atccctgcct tggaagctga gtaattagcc tgcccggcct
     1501 gaccggccag cccggagacg gacgataaat ttacgatccg cccgtaacgc tgtttgagca
     1561 tgctgcggat ggctgcttta caaaaattaa atgtaccctt taagtttgtg tccagtacga
     1621 tatcccaatc agcttcattc atacgcatga tcagattatc gcgggttgtt ccggcattat
     1681 ttaccaaaat atcgatccgc ccgaaatctg ccacgacctg ttcaaccagc gcttttgcct
     1741 gggctggatc ggcgacatcg accgcataac ccagaacgcg ggtgctgcac tgggcggcga
     1801 tttcctgtgc ggcagcctgg gattgttcca ggctgcggct ggccagcgcc acctgagccc
     1861 cgccggctgc caatgcttcg accactgctc gtcctatccc gcggctgcca ccggtgacca
     1921 gggcaaccct accattaaaa tccataccgg agtgctcctc gattgagatc aaccagaccc
     1981 gccagctctg tcgaaaatcg tttgggattg gctggcaatt agcgaaggaa attataccgc
     2041 gaagtatac
//
LOCUS       DATMXK010000550         5241 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_75106, whole genome shotgun sequence.
ACCESSION   DATMXK010000550 DATMXK010000000
VERSION     DATMXK010000550.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5241)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5241)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5241
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_75106"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(5..1123)
                     /locus_tag="VN363_09975"
     CDS             complement(5..1123)
                     /locus_tag="VN363_09975"
                     /inference="COORDINATES: protein motif:HMM:NF012738.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84886.1"
                     /translation="MTAEAQPISEPVKTKGTTSTFTRVARYMSLRVVMLVITTVIGVY
                     LTILIANMGGYVDTIMRNDIRERTNATVAALTANKSVSPEARRTMVEQMIALEEKRLG
                     LNTPFAVRSFRYLGNALTLNLGRATYMVSDNGSREVRNIILERLPATLLLMGSSQLFL
                     FFFSLFVALALSRAYGSFWDKLVITLSPTSSVPPWFYGIFFILIFAAVFKILPFGGLV
                     DAPPPDNTFDYTLSVLEHLILPGGSLVISSLFLSIYNWRTFFLIYSSEDYVDMAKAKG
                     LTSRDIERRYILRPTLPTIVTNFALLVIGLWTGATITETVFLWPGLGRTIFRAIGFYD
                     TPVIVGTTIIYAYLLAVTVFLLDFVYAIVDPRVKVGGS"
     gene            complement(1203..3815)
                     /locus_tag="VN363_09980"
     CDS             complement(1203..3815)
                     /locus_tag="VN363_09980"
                     /inference="COORDINATES: protein motif:HMM:NF012706.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter substrate-binding protein"
                     /protein_id="HWQ84887.1"
                     /translation="MKRTTFLVMSLLIVASMVLAACQPAATPVVTEAPAEPVATEAPA
                     EPVATEAPAEPVATEAPTEAPTEEPTPEPVVSTRKGGWLDEIVISVVEGDSAVTQLQA
                     GAIDIYANGLSSNDLPAIEEAGLAYSTASGLYYDLLFNPVGPEFTDGRFNPFSNRKIR
                     EAMNWLVDRDYLNQEIYAGGALPKWFAITTQFPDYADLADVARRLEAYYAYDMDKAKE
                     VIDAEMTTMGAELVDGKWNYGGEPVSLIFVIRNDSDGTRIPQGDYVAAQLESIGFTVD
                     RQYKTGSEASPIWIGSDPAEGQWHIYTAAWSATVLDRDQSNIFQEMYLDSSAQGIPAF
                     LANVSDPEFKEIGDKLANAEFATLEERHDLMARAMELSLQDSFQVFLIDGKNYIPYVE
                     NLVATSDLAAGVEGSSIYPFTLRFKDEEGGTVRWATQDLFAEPWNPIAGSNWAFDQGA
                     IRATNSAYGEVVYDPFTGLVWPMRMERADVVATEGLPIGKTLDWVTLEFAPEITVPED
                     AWVDWDATNQVFVTAAEKFPEGQTALVKTTQYYPADMYETVKWHDGSNLSPADFIMSM
                     IMTFDRAKPESAIYDEQAVPRFELFLESFKGVKFVSMDPLVVEYYTDNYALDAELNLG
                     VIFPTYTFAEGSWPMMAIGNLAEAAGEVAYSIDKAGTAEIEQTSYVGGPTLEILAKYL
                     DQAIAETTIPYAPTMSEYLTAEEATARYEAYKAWYGDHGHFWVGTGPYFLDQAFLTEK
                     SLVLKHFADYPDLASKWSAFGEPKLADVEIDGPGQVKIGEEAQFDVYVNFAEAPYPTA
                     EIKQVKYLLYNAQNEIVAVAEAEAVEDGLYKVVLTAETTSALEAGSNKLEVAVVPLTV
                     SQPTFTSVEFVTAP"
     gene            complement(4185..>5241)
                     /locus_tag="VN363_09985"
     CDS             complement(4185..>5241)
                     /locus_tag="VN363_09985"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWQ84888.1"
                     /translation="DLDPLTFAVVDNPAHGTLSGTAPDLIYTPDADFNGDDSFTFTVS
                     DGALVSAPAAVSITVEPVNDAPVAAAQAVSTSEDTPLAITLTGSDVDLDPLTFAVVDN
                     PAHGTLSGTAPDLIYTPDADFNGDDSFSFTVSDGALVSAPAAVSITVEPVNDAPLADS
                     QTVTTEEDTPLAITLSGSDVDGDSLTFALVADPAHGTLSGTAPDLLYTPAAGYTGMDS
                     FTFTVSDGTLVSAPAAVNITITPLNDAPVALNDQYTVNEDSVLTVLAAEGVLSNDTDL
                     DGDDLAALLVSGPLHGTLSLDAEGGFIYTPAADFNGEDSFTYRAYDGLEYSEPATVII
                     TVNEVPESLYLPVIAQP"
BASE COUNT         1099 a         1419 c         1627 g         1096 t
ORIGIN      
        1 atggctaact ccccccaact ttgacccgcg ggtcaacaat ggcgtaaaca aaatccagca
       61 ggaataccgt caccgccagc agataggcat agataatcgt cgtacccaca atgaccggtg
      121 tatcgtaaaa accaattgcc cggaagatcg tgcggcccag cccgggccat agaaatacgg
      181 tctccgtgat tgtggcgccg gtccacaggc caatcaccag taacgcaaag ttcgtcacaa
      241 tcgtcggcag ggtaggccgc aggatgtagc ggcgttcgat atcccgcgag gtcaggcctt
      301 tggctttggc catatccaca tagtcctcgc tggaatagat gagaaagaag gtgcgccagt
      361 tgtagatgct caggaacagc gaggagatga ccagcgagcc gccaggtaag atcaggtgtt
      421 cgagcacgct cagggtgtaa tcaaatgtat tatccggcgg cggggcatcg accagcccgc
      481 caaacggcag aattttaaac actgccgcaa agatcaagat aaaaaagatg ccatagaacc
      541 agggtgggac ggatgaagtt ggcgacagcg taatcaccag cttatcccaa aaactaccat
      601 aggcgcgcga caatgccagg gccacaaaca gactgaagaa gaagaggaat aattgcgagc
      661 tgcccatcag cagtaaggtc gccggcagcc gttcgaggat gatgttacgc acctcccgcg
      721 agccgttgtc gctgaccatg tatgtcgccc ggcctaaatt cagggttaaa gcatttccca
      781 gatagcgaaa tgagcgcaca gcgaagggag tgttcagacc caggcgtttt tcttccaggg
      841 cgatcatttg ttcaaccatg gtcctgcgcg cttcaggcga tacactctta ttggctgtca
      901 gagctgccac cgttgcattg gtgcgctcac ggatatcgtt gcgcatgatg gtgtcaacgt
      961 agccccccat attggcaatc aggatcgtca ggtagacccc gatcacggtg gtaattacca
     1021 gcatgaccac gcgcaggctc atatagcgcg ccactcgcgt gaaggttgag gtggtgcctt
     1081 tcgttttcac aggctcgctg atcggttgcg cttcagctgt catgtcttac ctcattcctg
     1141 gaatagatag gggcaagtga accacttgcc cctatcttca ttactggtaa acgggtaccg
     1201 tcttacgggg ccgtgacgaa ctccaccgag gtgaaggtcg gctggctgac ggtcagcggg
     1261 accacggcaa cttcgagctt gttggagcca gcttcgagag cgctggtggt ctcggcggtg
     1321 aggacgacct tgtacaggcc atcttcgacg gcttcggctt cggcaacggc gacgatctca
     1381 ttctgggcat tgtacagcaa gtacttcacc tgtttgatct cagccgttgg atatggagct
     1441 tcggcaaagt tcacgtaaac atcgaattgg gcttcttcgc caatctttac ctggccgggg
     1501 ccatcgatct caacatcggc cagcttgggc tcgccgaagg cggaccactt ggaagccagg
     1561 tcggggtaat cggcaaagtg cttgagcaca aggctcttct cggtcaggaa agcctggtcg
     1621 aggaaatacg ggccggtgcc gacccagaag tgaccatgat cgccgtacca ggccttgtag
     1681 gcttcatagc gagcggtggc ttcttctgcg gtcaggtatt cgctcatggt gggagcgtag
     1741 gggatggtgg tctcggcgat tgcctggtcc aggtatttcg ccaggatctc gagggtgggg
     1801 ccgccaacat agctggtctg ttcgatttca gcggtgccgg ccttgtcgat cgagtaagca
     1861 acctcgcccg cagcttcggc aaggttaccg atggccatca tcggccagga gccctctgcg
     1921 aaggtgtagg tcgggaagat cacgccaagg ttgagctcgg catccagggc atagttgtcg
     1981 gtgtagtact cgaccaccaa cggatccatg gacacgaact tgacgccctt gaaggattca
     2041 aggaacagct cgaagcgcgg gacagcctgc tcgtcataga tggcggattc gggcttggcg
     2101 cgatcgaagg tcatgatcat gctcatgatg aaatcggccg gggacagatt gctgccatcg
     2161 tgccatttca cagtctcgta catgtcggca ggatagtact gggtggtctt aaccagggca
     2221 gtctgacctt cggggaattt ctcagcggcg gtgacgaaga cctggttggt ggcatcccag
     2281 tcaacccagg catcttcggg gacggtgatc tcaggagcga attccagggt cacccaatcc
     2341 agggtcttgc cgatcggcaa accttcggta gcgaccacat cggcgcgttc catacgcatc
     2401 ggccagacca ggccggtgaa gggatcgtac acaacctcgc cgtaggcgct gttggtggcg
     2461 cgaatggcgc cctgatcaaa ggcccagttg gagccggcga tcgggttcca gggttcggcg
     2521 aacaggtcct gggtagccca gcgcacggtc ccgccttcct cgtccttaaa gcgcagggtg
     2581 aaggggtaga tcgatgagcc ttcaacgccg gcagccaggt cggaggtggc aaccagattt
     2641 tcgacgtagg ggatatagtt cttaccatcg atcaggaaga cctggaagga gtcctgcagg
     2701 gagagctcca tggcacgcgc catcaggtca tggcgttctt cgagggtagc gaattcagca
     2761 ttggccagtt tgtcgccgat ttccttgaat tcaggatcgg aaacgttggc caggaaggcc
     2821 gggataccct gggcactgga atccaggtac atttcctgga agatgttgct ctggtcgcgg
     2881 tccaggacgg tggcgctcca ggcagcggtg tagatgtgcc actggccttc agcggggtcg
     2941 gaaccgatcc agatcgggga ggcttcggag ccggtcttgt actggcgatc tacggtgaag
     3001 ccgatgcttt ccagctgcgc ggcaacgtag tcgccctgcg ggatgcgggt gccatctgag
     3061 tcgttgcgga tgacgaagat cagggaaaca ggctcgccgc cataattcca cttgccatcc
     3121 accagctcag cgcccatggt ggtcatttcg gcgtcgataa cttctttggc cttatccata
     3181 tcgtaggcgt agtaggcttc caggcggcga gctacatcag ccaggtcggc gtaatctggg
     3241 aactgggtgg tgatggcgaa ccatttcggt agcgcgccac cggcatagat ttcctggttg
     3301 aggtaatcgc gatcgaccag ccagttcatg gcctcgcgga tcttgcgatt gctgaatggg
     3361 ttgaagcggc catcggtgaa ttctggccca accgggttga agagcaggtc gtaatacaga
     3421 ccactggcgg tggagtaggc caggccggct tcctcaattg cgggcaggtc gttggaggac
     3481 aggccattgg cgtaaatatc aatggcgcca gcctgcagct gggtgacggc ggaatcgcct
     3541 tccacaaccg agatcacgat ctcgtccagc cagccgcctt tgcgggtaga cacgacgggt
     3601 tctggagtgg gctcttcagt tggcgcttct gtgggagcct cggtagccac gggttcagcc
     3661 ggggcttcgg tggcgacggg ctcggcggga gcctcagttg ccaccggttc tgctggcgct
     3721 tcggttacga ccggagtcgc agcaggttgg caggcggcca gaaccatgct cgcaacaatg
     3781 agcaagctca tgaccaaaaa ggtggttcgc ttcatttctc tctcctttag attggttaca
     3841 tactgatcta gatacggttc gacgctgagg atttctcttc cgtttttggt tggcacccgg
     3901 tttctacccg gaccggacgc tggtcccagg cggtgcgtct ccccatacca caatatcccc
     3961 ggttaggggt atccgacgaa tatagcataa gagactgcat tcgtcaagaa tcacagactg
     4021 taatgataaa ttgttcattt tagctgaaca actctttttg cattgaagtt tctggaggtg
     4081 atcacgacct atacaatata ctatcgatca ggcacagagg atcaatccgc gcagcagggg
     4141 cggagcgaat gggctccgcc cctgctggaa aagggatggg tgacttatgg ttgagcaatt
     4201 accggcaggt acagcgattc tgggacctcg ttgaccgtga tgatcacggt tgcaggctca
     4261 gaatattcca ggccatcgta ggcccggtag gtgaagctgt cctcgccgtt gaaatcggcg
     4321 gccggggtat agatgaatcc accttctgcg tccagactga gcgtgccatg cagggggcca
     4381 ctgaccagca gcgccgccag gtcatcgcca tccaggtcgg tgtcattgct caatacgcct
     4441 tccgcagcca ggacggtcag aacactgtct tcattaacag tgtactggtc gttcagggca
     4501 accggggcgt cgttcagggg cgtgatcgtg atgtttactg cggccggagc ggagacaagc
     4561 gtaccgtcgc ttaccgtgaa ggtgaagctg tccataccgg tgtaacccgc ggcaggcgtg
     4621 tagagcaggt cgggcgctgt accgctgagg gttccatgcg ccggatcggc caccagggca
     4681 aaggtcaggc tgtcgccgtc cacatccgac ccgctcaggg tgatcgccag cggcgtatcc
     4741 tcctcggtcg ttaccgtctg cgagtcggcc agcggggcgt cattgaccgg ttcgacggtg
     4801 atgctcaccg ccgccggagc agacaccagt gcgccgtcgc tgacggtaaa ggaaaagctg
     4861 tcgtcgccgt tgaagtccgc gtctggggtg tagatcaggt cgggagcagt gccgctcagc
     4921 gtcccatggg ccgggttatc caccacggca aaggtcagcg ggtcgagatc gacatccgag
     4981 ccggtcaggg taatggccag gggagtgtcc tcgctggttg aaactgcctg agccgccgcc
     5041 accggggcgt cattgaccgg ttcgacggtg atgctcaccg ccgccggagc agacaccagt
     5101 gcgccatcgc tgacggtaaa ggtgaagctg tcgtcgccgt tgaagtccgc gtctggggtg
     5161 tagatcaggt cgggagcagt gccgctcagc gtcccatggg ccgggttatc caccacggca
     5221 aaggtcagcg ggtcgagatc g
//
LOCUS       DATMXK010000551         3083 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_5208, whole genome shotgun sequence.
ACCESSION   DATMXK010000551 DATMXK010000000
VERSION     DATMXK010000551.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3083)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3083)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3083
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5208"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            108..356
                     /locus_tag="VN363_09990"
     CDS             108..356
                     /locus_tag="VN363_09990"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84889.1"
                     /translation="MPEKYVIRIQGLLDQHWAEWFAGLQLSHLAGDVTLLTGILPDQA
                     ALHGLLERIRDLNLTLISVTRCDPASQDPGQECSPTDQ"
     gene            453..704
                     /locus_tag="VN363_09995"
     CDS             453..704
                     /locus_tag="VN363_09995"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84890.1"
                     /translation="MAKSGKWLLIRVTDLQTGEKKTNVRIPVSLANFGIKMANKYAPE
                     SLEGLDMDEIMAALREGDEGILVDVEDEQSGDHVEILVE"
     gene            800..1390
                     /locus_tag="VN363_10000"
     CDS             800..1390
                     /locus_tag="VN363_10000"
                     /inference="COORDINATES: protein motif:HMM:NF024135.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="flavodoxin domain-containing protein"
                     /protein_id="HWQ84891.1"
                     /translation="MEMTILVTYASRNGATREIAERIGGVLRRAGLEVDVAPVDTIRS
                     LSPYQAVILGSAIYIGKWQKDATVFLQENAQALADRPTWLFASGPTGLGDPVSLVEGK
                     ILPANLEPLVERIHPRAVAIFHGCIAPEKISFIEKSVIKGMHKPFGDFRDWEMIETWA
                     AMVAAQLAAQSAAQAAAGLSLEMNAARSGSLIHQPL"
     gene            1433..2371
                     /locus_tag="VN363_10005"
     CDS             1433..2371
                     /locus_tag="VN363_10005"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84892.1"
                     /translation="MQYPKRILAAAIVLALVTASCSITEKLSSTSFKTGPTQEIAIQV
                     PLPQAAALSAGAGDVSLTLDFVAGVLKLAPADSKALTAGSSGNLASGDSALLASGLAT
                     FNVAEFAPIVASQGSAYTVKTGDMKIKGIPKFADDLINIWDLQLASTPMSLKILAGGY
                     SGCFELGGLALQNLEIEEGGSQLTSAFSAPNQVEMESLLVSSGGSNMTLTGLANANFA
                     RMTLSAGAGTYTLSFDGELQRAASVAIDIGASTVNLTVPDGVSASLTFNGGLSSVNAG
                     AGWELVNNVYTHPGRGPALTISVEMGVGTLNLNPEG"
     gene            2470..>3083
                     /locus_tag="VN363_10010"
     CDS             2470..>3083
                     /locus_tag="VN363_10010"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ84893.1"
                     /translation="MLIRDISLYVKVTGHGEPLLLIHGGPGLDHTSLLPLAPLADRYT
                     LIFYDQRCNGRSVGPDVASMTWENLTADAEALREILGFDRWAVLGHSFGGHVALEYAL
                     RHPESISRLILMDTAGESRWSMENAPQLLARRGYRPAAVNAVRRFFNGQVTPAEFVPT
                     VMQFFTAYYAKFSPLTLAHDLFSGRKMVMQPEAQISFCRDTFPG"
BASE COUNT          640 a          976 c          867 g          600 t
ORIGIN      
        1 ccccacaaat aaaccctcca ataaaccccg aacctcccat ctggcagatg cggggagctt
       61 cgtcgcgcat taaactggtt acaacaagca agtaaaggag ggcagatatg ccagaaaaat
      121 atgtaatcag aatccaggga cttcttgacc agcactgggc ggagtggttc gccggcctgc
      181 agctttctca cctcgccggg gatgtaaccc tgctcaccgg catcctgccc gaccaggccg
      241 cccttcacgg gctgctcgag cgcatccgcg acctcaacct gaccttgatt tcggtcaccc
      301 gctgcgatcc agccagccag gatcccggtc aggaatgttc accaaccgat caataacagt
      361 ctcactgagc cagccgtgtg cgccgctggt gaaactgtcc aaataaaact cgcaagagtg
      421 caaaacggtc gttacgacca gaggagatca aaatggcaaa gtcaggtaag tggttactca
      481 ttcgtgtgac ggatttacag actggagaga agaaaaccaa cgtccgcatc ccggtcagtc
      541 tcgccaattt tgggatcaag atggcgaaca agtacgcccc cgaaagcctc gaaggtctgg
      601 acatggacga gatcatggcg gccctccggg agggtgatga aggcatcctg gtagatgtcg
      661 aagacgaaca gagcggcgac cacgtcgaaa tcctcgtcga ataaagtccc ccggcgcagc
      721 ctggcgtctc ggtcggcgtg ttaggccgga cgccaggtgc ccataatgct cgcagctcga
      781 gaatgcaagg aagaataaaa tggaaatgac gatcctggtt acttatgctt cacgcaacgg
      841 cgccacccgc gagattgccg agcgtatcgg cggcgtgctg cgccgcgccg gcctggaagt
      901 ggatgtcgcc ccggtggata caatccgcag cctgagcccc taccaggctg tgatcctggg
      961 cagcgccatc tacatcggca aatggcaaaa agatgccacc gtcttcttac aggagaatgc
     1021 acaggctctg gcagaccgcc ccacctggct cttcgccagc ggacccaccg gtctgggtga
     1081 tcccgtctcc ctggtggagg gcaagatcct gccagccaac ctggagccgc tcgttgagcg
     1141 catccatccc cgtgccgtcg ccatctttca cggctgtatc gccccggaga agatcagctt
     1201 cattgaaaaa tcggtcatca agggcatgca taagcccttt ggcgactttc gggattggga
     1261 gatgatcgaa acctgggcgg cgatggtcgc ggctcagctc gctgctcagt ctgcggccca
     1321 ggccgcagcc gggctcagcc tggaaatgaa cgccgccagg tctggttccc tgatccacca
     1381 gccgttgtag gcgaccccgg ctgaaaagtt tgtttttgtc tggagaaatc tcatgcagta
     1441 ccctaaacga attctggctg cagcgatcgt cctggccttg gtcacggctt cctgcagtat
     1501 taccgaaaaa ttgtcctcta ccagcttcaa aactggaccc acccaggaaa tcgcgatcca
     1561 ggtccccctg ccccaggcag ctgccctgtc cgccggagcg ggcgatgtct cgctcaccct
     1621 ggattttgtc gccggtgtct tgaagctggc ccctgcagat agcaaggcgt tgacagccgg
     1681 tagtagcggc aacctggctt caggagacag cgccctgctg gcctccggcc tcgccacatt
     1741 caatgtcgcc gagttcgccc ccatagtcgc atcgcaaggc tcagcttaca ccgtgaagac
     1801 tggcgacatg aagatcaagg gcatcccgaa atttgccgat gatctcatca atatctggga
     1861 tttgcagctt gccagcacgc ccatgagcct gaaaatcctg gccggcggtt acagcggctg
     1921 cttcgagctc ggcgggctgg cccttcaaaa tcttgagatc gaagagggcg gctcgcagct
     1981 taccagcgcc ttctcagccc ccaaccaggt tgaaatggag tccttgctgg tctcctcggg
     2041 cggctcgaat atgaccctga ccggcctggc gaacgccaac tttgccagga tgaccctgag
     2101 cgccggggca ggcacctaca ccctgagctt cgatggcgag ctgcagcgcg cggccagtgt
     2161 ggctatcgat atcggcgcca gcaccgtcaa tctcaccgtc ccggatggcg tcagcgcctc
     2221 gctcaccttc aatggcgggc tgtcctcggt caatgccgga gcaggttggg agctggtcaa
     2281 caacgtctac acccatcccg gccgcggccc ggccctcacc atctcggtcg agatgggcgt
     2341 gggcaccttg aacctgaatc ctgaaggata gaaccctgcc ggctctctcg ctgggcaagg
     2401 caagcctgcc tgcccggcgg agagcagcgg cgcccaggaa aatggcactg caaggaaaag
     2461 agcaccccca tgttaatccg cgatatatcc ctttacgtaa aagtcaccgg acacggcgag
     2521 ccgctgctgc tcatccacgg cggtccgggc ctggatcaca ccagcctcct gcccctggcg
     2581 ccgctcgccg accgctatac cctgatcttc tatgaccagc gctgcaacgg ccgttccgtg
     2641 gggccagatg tggcctcgat gacctgggaa aacctgaccg cagacgccga agctctgcgc
     2701 gagattcttg gctttgaccg ctgggcggtt ctcgggcatt ccttcggcgg gcacgtcgcc
     2761 ctggagtatg ccctgcgcca tccggagagc atttcacggt tgattttgat ggataccgct
     2821 ggcgaatcgc gctggtccat ggagaatgca ccccagctcc tcgcccggcg cggttaccgg
     2881 ccggccgcag tcaatgcggt gcgccgtttc ttcaacggtc aggtgactcc cgccgagttc
     2941 gtccccacgg tcatgcagtt cttcaccgct tactatgcga aattcagccc cctgactctg
     3001 gcccatgatt tattttctgg tcgaaaaatg gtgatgcagc ctgaggcgca aatttctttc
     3061 tgccgagaca cctttcccgg ctg
//
LOCUS       DATMXK010000552         9435 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_52391, whole genome shotgun sequence.
ACCESSION   DATMXK010000552 DATMXK010000000
VERSION     DATMXK010000552.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 9435)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 9435)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..9435
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52391"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1269)
                     /locus_tag="VN363_10015"
     CDS             complement(<1..1269)
                     /locus_tag="VN363_10015"
                     /inference="COORDINATES: protein motif:HMM:NF025711.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CpXC domain-containing protein"
                     /protein_id="HWQ84894.1"
                     /translation="MPATRVSCPNCRQPVTVDVQQVFDLNQEPDAKQRLMSGTVNVIQ
                     CPNCRYQGNLATPILYHDPEKELFLTYVPPEMGLPLPEQERLLGSLMNQVINKLPQEK
                     RKGYLLRPQPAFTYQGLVERILEADGITKEMLDRQQKRLALLQRLVSASSPDVMAEIA
                     KQEDAMIDQEFFGILSRLVEVSGASGDEQSARALTELQRQLLPITTFGRELQSQTKEI
                     EAAMQDLRALGNELTREKLLDLVIAAPNETRLSALVSMARAGFDYQFFQLLSETIDRA
                     PEGERARLVELREHVLKITSEIDRQMNERMQAASQLLEKVLQAPNVEEALMENLPGVD
                     DFFVQEVNRSLDAARKQGDLERSGKLQQIIAALQSMEEAPPEVAFIEELMQAPDDASR
                     LQLLESHAEEVTPEFLNLLMNFAGQMDEQQP"
     gene            complement(1303..2877)
                     /locus_tag="VN363_10020"
     CDS             complement(1303..2877)
                     /locus_tag="VN363_10020"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ84895.1"
                     /translation="MLPDFRVRQRDYLLEISRAITQELDLDKLLDRILTVSVEMLAGQ
                     AGLIALRASPSGWSLGASYGVPPAFLRYFEPLLAEIPENEDSSTTELAEINRLLQNIT
                     RATSLGLLTGVVLPLFAQKRVIGLIFIFRNYAGSFSVNDRALLQSFADQAAIAVHNAQ
                     LYTQVSLEKSRLDALVASAADGILILKSDQTVEQCNSAFASMVNQSTSGLIGLPHAEV
                     VRFTSVRDGLTLEQAVLGGWPLTPHATLYVEGDLQRSNGQTIPIGVTYAPLLDAQGQL
                     TNIIATARDITRFREADELKSTFISVVSHELKTPIALIKGYVSTLRRDDANWDRAIVD
                     DSLEVIEEEADHLTELIENLLDASRLQAGALSINRSDVALDVLATRTAERFRTQTDRH
                     NFQVEFPPSFPVILGDEDRLGQVLDNLLSNAIKYSPNGGEITVRGEVRSTQVIVCVSD
                     QGPGIPTQDIPHIFDRFYRASETARTTKGAGLGLYLARAVVEAHGGRIWVDPAPGSGA
                     RICFSLPRDESPGALR"
     gene            complement(2867..4201)
                     /locus_tag="VN363_10025"
     CDS             complement(2867..4201)
                     /locus_tag="VN363_10025"
                     /inference="COORDINATES: protein motif:HMM:NF004426.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="acetyl ornithine aminotransferase family
                     protein"
                     /protein_id="HWQ84896.1"
                     /translation="MQPTIKLGPKARAYIERDSAVISPSYPRGYPFVMDHGRGTEVWD
                     VDGNRFLDFAAGIAVVSTGYSHPRVVQAIQEQAEKFIHVSSDFYHEKWIELAERINEL
                     APMSGDVVSFMTNSGTEAVEAAIKLARYHTRRTEFIGFLGAFHGRTIGSVTFTASKPG
                     YHRNFYPLMNGVYHVPFPDPYRPILQSLPGEAYGETVVRYLEEQIFGHLVPADNVAGI
                     LLEPIQGEGGYIVPEASFFPRLRQLCDKYGILLIVDEVQSGVGRTGKMWAIEHFGVEP
                     DIVCSAKGIASGMPLGVMFARKEIMNWPRGSHGNTYGGNPIACAAALATLDLVENGYM
                     QNAQEMGVYAMECLHQIQKAHPSLGDVRGEGLMIGLDFVKDPQTHAPDGNLRDRVVDM
                     AFEYGLLLLGCGPSVIRLAPPLSVNRAEMDEAFEVLDYVIGVCEKGTLPVAA"
     gene            4385..5545
                     /gene="tgt"
                     /locus_tag="VN363_10030"
     CDS             4385..5545
                     /gene="tgt"
                     /locus_tag="VN363_10030"
                     /EC_number="2.4.2.29"
                     /inference="COORDINATES: protein motif:HMM:TIGR00449.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0008479 - tRNA-guanosine(34) queuine
                     transglycosylase activity [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA guanosine(34) transglycosylase Tgt"
                     /protein_id="HWQ84897.1"
                     /translation="MSFEFEIQARQECARAGVFHTPHGELLTPVFAPVGTQASVKAIT
                     PAQLSELGASLVLANTYHLYLRPGDDLVAEMGGLHQFMHWDRPMLTDSGGFQVFSLAP
                     SRKIDADGVTFKSHIDGSIHRLTPEKSIAIQENLGADIIMAFDECAPPYDREYSERAM
                     ARTHSWLERSLQAKIRPDQALFGIVQGGIFPDLRQASAEFVASLNLPGTAIGGLSVGE
                     TKAEMNAILEVVDPLLPVDKPRYLMGVGSPEDLVNGVLRGIDIFDCVLPTRLARHNAA
                     MTRTGRLNLVNATYAHDPLPIDESCTCYTCQNFSRAYIRHLITAREMLVATLLSIHNL
                     HTLLSLMADLRQAIFAGKLEAFTADFFAARKGASENGLLAGLPETEPENPPF"
     gene            5569..6390
                     /gene="uppP"
                     /locus_tag="VN363_10035"
     CDS             5569..6390
                     /gene="uppP"
                     /locus_tag="VN363_10035"
                     /EC_number="3.6.1.27"
                     /inference="COORDINATES: protein motif:HMM:NF014703.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030288 - outer membrane-bounded
                     periplasmic space [Evidence IEA];
                     GO_function: GO:0050380 - undecaprenyl-diphosphatase
                     activity [Evidence IEA];
                     GO_process: GO:0009252 - peptidoglycan biosynthetic
                     process [Evidence IEA];
                     GO_process: GO:0046677 - response to antibiotic [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="undecaprenyl-diphosphatase UppP"
                     /protein_id="HWQ84898.1"
                     /translation="MTLFQSILLGIIQGLTEFLPISSSGHLVIVPHLLGWDIPGDEAF
                     VFNVLVQVATIIAVIAYFWKDLVTILSAVFSGLARRTLLANPQARLGWMIVAATIPAG
                     LVGLLIKDLVEQAFSSLLATGIFLLVTAGLLTIAERMGKRQRELDSLKLSDAVWIGVA
                     QAAAIFPGVSRSGATITGGMLRGLQRPAAARFSFLMSIPIMLAAGLLAFLDLLEMPNI
                     GRLLPVFIPGFLAAGVTGYFAIRWLLGYLTRKPLTIFSIYCLALGLLSIGVSLFG"
     gene            6383..7174
                     /locus_tag="VN363_10040"
     CDS             6383..7174
                     /locus_tag="VN363_10040"
                     /inference="COORDINATES: protein motif:HMM:NF024257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="substrate-binding domain-containing protein"
                     /protein_id="HWQ84899.1"
                     /translation="MVDPHFRRWLLHSLLAGLALLLAACRPATSLPDPTEFPEAVLVQ
                     YTPALRILTPVLEACAAEDPDLALFLEETPKPAIDLSSDPDSPVRLGLALGEPLAQNY
                     SAALTDAQIAVITHPSNPIQEISAAGLQELFSGQIQNWAAIGGSDQTVTPWVLTPADE
                     ARQLFEKAILGESKVSGQARLAADPAWMLASVSTDPGAVGYVPAAWLNDSVNVLKLAQ
                     GLSEELRLPLLALAGSEPQGAARRLLVCLQSGSGQAALKMLFQAP"
     gene            complement(7171..7530)
                     /locus_tag="VN363_10045"
     CDS             complement(7171..7530)
                     /locus_tag="VN363_10045"
                     /inference="COORDINATES: protein motif:HMM:NF012753.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030234 - enzyme regulator activity
                     [Evidence IEA];
                     GO_process: GO:0006808 - regulation of nitrogen
                     utilization [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="P-II family nitrogen regulator"
                     /protein_id="HWQ84900.1"
                     /translation="MSYLVVMIVDDPDDCPAILDAWETLGVSGVTILESSGLGHLRKL
                     GMREDMPLLPSLSDFMQQDEIPHRMLLSVVKDQEMVDRMITTVQHISGDLDQPNSGFL
                     FVVPVLQAIGLNRKLED"
     gene            complement(7527..8762)
                     /locus_tag="VN363_10050"
     CDS             complement(7527..8762)
                     /locus_tag="VN363_10050"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWQ84901.1"
                     /translation="MSNFLQLALALAGIIALAKIGGLISIRLGQPSVLGELIVGILLG
                     PTLIDFLHLPLFTSSHLSETIHELAEIGVLLLMFIAGLDLHLEDLTRSFKVSALAGTL
                     GVILPLALGAGLGTLFNMNGQEAFFIGLILAATSVSISAQTLMELKMLRTRVGTSLLG
                     AAVFDDILVILGISIFIAVTSSEPNFLDILWIVVRMALFLGIGTILGMKLLPRLSQRV
                     AGRDIAQGVVSFTFIIVLIYGIAAETLGGMAAITGAFIAGLALTRSPVRERIRNGTSV
                     LAYGVFVPIFFVNVGLAVDARQLFGPVFWLFIGMTVVAVIGKVVGAGAGALWGGLNRM
                     EALQIGVGMMSRGEVGLIVASIGVTQGLVSENTLVAIVGVVIITTLLTPPLLRSLFAK
                     PHPQSQTQIAETSPEGDSK"
     gene            complement(8839..>9435)
                     /locus_tag="VN363_10055"
     CDS             complement(8839..>9435)
                     /locus_tag="VN363_10055"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84902.1"
                     /translation="AKEAAMPDIEYDLRYLAAGLEGLDEYLHSDEVYWPLQATPLAGQ
                     PPFPQLTLDGLLLSAARLQAQVLPDSHQAEYQQLVNQLEAVRSKRRVAWEKKATHNFR
                     ARLTLWRNFMVELRDETDANADRYPYEVTRRVQLTLLSEDARQRPTAEDELVRMLDSL
                     LETLLAPGAFIWDPELERGFPQSRFWFLYGQIKPQAKD"
BASE COUNT         2092 a         2739 c         2576 g         2028 t
ORIGIN      
        1 aggttgttgt tcatccatct ggccggcgaa attcattaat agattcagga attccggggt
       61 gacttcctcg gcgtgactct caagcagctg caggcgggaa gcgtcatcgg gagcctgcat
      121 cagctcttcg ataaaagcca cttcgggcgg ggcttcttcc atactctgca aagcggcaat
      181 gatttgctgc aatttccccg atcgttccag atcgccctgc ttgcgggccg catccaggct
      241 gcgattgact tcttgcacga agaaatcatc cacacccggc agattctcca tcagggcttc
      301 ctcgacattt ggcgcctgta agaccttctc caggagctgg ctggcagctt gcatgcgctc
      361 attcatctgg cggtcgatct cagatgtgat cttcaagacg tgctcgcgca gctcaaccag
      421 acgggcgcgc tccccttccg gggctctgtc gatcgtctcg ctgagcagct ggaagaattg
      481 atagtcaaaa ccagcccggg ccatgctcac cagagcgctc aggcgggttt catttggagc
      541 agcgataacc aggtcgagca gcttctcgcg agtcagctca tttcccaggg cgcgcaaatc
      601 ctgcattgcg gcttcgattt ctttggtctg gctctgaagc tcccgtccaa atgtcgtgat
      661 cggcagaagc tggcgctgca gctctgtcag ggcacgcgcg ctttgctcat cgccacttgc
      721 cccagacact tccaccaggc gggaaaggat cccaaagaac tcctggtcga tcatggcatc
      781 ttcctgtttg gcaatctcag ccatcacatc tggcgaagaa gcgctgacca gccgctgcaa
      841 taaggccaga cgtttctgct gcctgtccag catttctttg gtgatcccgt ccgcttccag
      901 gatgcgttcg accaggcctt gataagtgaa cgccggctgg ggtcgcagga gataaccttt
      961 tcgcttctcc tgggggagtt tattaatcac ctggttcatc aacgagccga gcagccgctc
     1021 ctgttcaggc aggggtaagc ccatctcggg tgggacatag gtcagaaaaa gctctttttc
     1081 cgggtcgtgg tacaggatgg gagtagcaag attaccctga tagcggcaat tcgggcactg
     1141 gataacattc acagttccgc tcattaaacg ctgtttggca tctggttcct ggttcaggtc
     1201 aaagacttgc tgcacatcca ctgtgacagg ctggcggcag ttagggcaac ttacacgagt
     1261 agctggcata aggacactcc aaaaagaaaa gccggtttaa gattagcgca gtgcgcccgg
     1321 gctttcatca cgaggtaaag aaaaacagat ccgcgctcca gatccaggag ccgggtccac
     1381 ccagatccgt cctccgtgcg cttcgaccac tgcccgggcc aggtagagac ccaacccggc
     1441 acctttggta gtgcgagcag tttccgaggc gcggtaaaag cggtcaaaga tatgtgggat
     1501 atcctgagtt ggaattcccg gtccctggtc gctgacacaa acgatcacct gcgtggagcg
     1561 aacctctcca cggacagtga tctcaccccc attgggggaa tatttgatgg cgtttgaaag
     1621 cagattatca agcacctgtc ccaggcggtc ttcatcgccc aggatgactg gaaaactggg
     1681 cggaaattct acctgaaaat tatgccgatc cgtttgagtt cgaaagcgct cggcagtgcg
     1741 cgtcgccagc acatccaggg caacatccga gcgattgatc gagagtgccc ccgcctgcaa
     1801 gcgggaggca tccagcaggt tttcgatcaa ttcggtgagg tgatcagctt cttcttcgat
     1861 cacctcgagg ctgtcatcga caatcgcacg gtcccagtta gcatcatccc ggcgcagggt
     1921 gctgacataa cctttgatca gggcaatcgg tgtctttagc tcgtggctga cgacagagat
     1981 gaaagtgctt ttcagctcat ctgcttcgcg gaagcgggtg atatcgcgtg ccgtggcaat
     2041 gatgttggtc agctgtccct gggcatccag caatggtgca taggtcactc cgatcggtat
     2101 agtctgtcca ttcgagcgtt gaaggtcgcc ttccacgtac agggtagcat gcggggtcag
     2161 cggccagccg cccagcacag cttgctccag ggtcaaacca tcgcgcacgg aagtaaacct
     2221 gaccacctct gcatgcggca ggccaatcag cccggaagtg ctctggttta ccatgctcgc
     2281 aaaggctgaa ttgcattgct cgacagtctg gtcggacttc aagatcagaa tgccatcggc
     2341 cgccgaagcg accagagcat ccaggcggct cttttcaaga cttacctggg tgtacagctg
     2401 agcattatga accgcgatcg ctgcctggtc ggcaaaactt tgcaggagcg cccgatcgtt
     2461 gaccgagaaa ctcccggcat aattacggaa gataaagatc aaacctatca cccgcttttg
     2521 ggcaaataag ggaaggacaa cccctgtcag caggcccagg ctggtggcgc gggtaatatt
     2581 ttgcagcagg cgattaatct ccgccagctc ggttgtggag gaatcttcgt tctcagggat
     2641 ctcggctaat aggggctcaa aataacgcaa gaacgcgggg ggaacgccat agctggcgcc
     2701 taacgaccag ccgctgggac tggcgcgcaa agcgatcaat ccagcctggc cggccagcat
     2761 ctcgaccgaa acggtgagga tgcgatccag gagcttatcc agatccagct cctgagtgat
     2821 cgcccggcta atctcgagca ggtaatcacg ctgtcgaacg cgaaaatcag gcagcaaccg
     2881 gtaaagtccc tttctcgcat acgccaatca cgtaatcgag gacttcgaaa gcttcatcca
     2941 tttcggcgcg attaacgctc agcggcgggg caaggcggat tacacttgga ccgcagccca
     3001 gcaagagcaa accatactcg aaggccatgt ccaccacccg gtcgcgcagg ttgccatcag
     3061 gcgcatgggt ctggggatct ttgacgaagt cgagcccgat catcaatcct tcgccgcgca
     3121 catcgccgag agaggggtga gccttctgga tctggtggag acattccatg gcataaacgc
     3181 ccatttcctg agcattttgc atataaccat tctcgaccag gtcgagggtc gccagggcag
     3241 ccgcgcaggc gataggatta ccaccatagg tattgccgtg cgagccgcgc ggccagttca
     3301 ttatctcttt gcgggcaaac atcactccca gtggcatccc cgaggcgatc cctttggcag
     3361 agcacacgat atccggctcg acgccgaaat gctcgatcgc ccacatcttt ccggtgcgcc
     3421 ccacgccgga ttgaacttca tccacaatca gcaggattcc gtacttatcg catagctggc
     3481 gcaggcgggg aaagaaggaa gcttcgggaa caatatagcc gccttcaccc tggatcggct
     3541 ccaggaggat gccggcgaca ttgtccgccg ggaccaggtg cccaaagatt tgctcttcca
     3601 ggtagcgcac cacagtttcg ccgtatgctt cgccagggag cgactgcagg atcgggcgat
     3661 acggatcggg aaaggggaca tggtaaaccc cattcatcag gggatagaaa ttacggtggt
     3721 aacccggttt actggctgtg aatgtcactg agccaatcgt ccgcccgtgg aaagctccca
     3781 gaaaaccaat gaattcggtg cggcgggtgt gatagcgggc cagcttgatg gctgcttcaa
     3841 cagcttctgt ccccgagttc gtcataaagg agaccacatc cccggacata ggcgccagtt
     3901 cattgatcct ttcagccagc tcgatccatt tttcatggta gaaatccgaa gaaacatgga
     3961 tgaacttttc ggcttgctcc tggattgcct gaaccaccct ggggtgactg tacccggtgg
     4021 agacgactgc aatcccggcg gcaaaatcca gaaaacgatt cccatccaca tcccatacct
     4081 cggtaccgcg cccgtgatcc atcacaaagg gatagccccg cgggtaggag ggagatatta
     4141 cagctgaatc tcgttcaatg taggcccgcg cctttggccc gagtttgatg gtcggctgca
     4201 taaattattt ctccttctaa tgtgctttgt tgtagagacg agcagatcgc gttgacccag
     4261 ccctgtacgg tccgaagtat tatatcattg ctcttcacac caaattttga cggataaaac
     4321 aggtataatc cccccttggc gtgcctgacg tgcgccaatc tgaaacaggg actacacacc
     4381 gacgatgtcg ttcgaattcg aaattcaagc ccgccaggag tgcgcccgtg cgggggtttt
     4441 tcacacgccc cacggcgagc tgctgacgcc agtctttgcg ccggtaggaa cccaggcgag
     4501 tgtaaaagcc atcaccccgg cccaattgag cgagctggga gccagcctgg tgcttgcaaa
     4561 tacctatcac ctgtacttac gtcctggcga tgacctggtc gccgagatgg gcggactgca
     4621 ccagttcatg cattgggaca ggcccatgct caccgattca ggcggctttc aggtattctc
     4681 cctggcgccg tctcgcaaaa tcgacgccga tggggtgacc tttaagagcc atatcgatgg
     4741 atcgatacac cgcctgaccc cggagaaatc aatcgccatc caggaaaacc tgggtgcgga
     4801 tattatcatg gcattcgacg aatgcgcccc gccttacgat cgagaatatt ctgagcgggc
     4861 tatggctcgc acccactcct ggctggaacg cagcctgcag gctaaaatcc gcccggacca
     4921 ggctttgttc ggtatcgtcc agggcgggat tttccccgat ttacgccagg catcggcgga
     4981 atttgtcgcc agccttaatc ttcctggcac ggcaattggc gggctgagtg ttggcgaaac
     5041 gaaggctgag atgaacgcca tactggaagt tgtcgatccg ctgctgcccg ttgataaacc
     5101 ccgctatctg atgggggtgg gctcgccgga agatctggtg aatggtgtcc tgcgcgggat
     5161 tgatatcttc gactgcgttc tgcccacccg cctggcgcgc cacaatgccg caatgacccg
     5221 caccgggcgc ttgaatctgg tcaatgccac ctacgcccac gatccgctcc cgatcgatga
     5281 aagctgtacc tgctatacct gccagaattt cagccgcgct tacatacgcc atctgatcac
     5341 agctcgcgaa atgctcgttg cgactctgct ttcgatccac aacctgcata ccttactgag
     5401 cctgatggca gacctgcgcc aggcgatctt tgccggtaaa ctggaagcct ttacagccga
     5461 tttcttcgcg gctcgcaagg gtgcatctga aaacggtctc cttgcaggtc tgcctgaaac
     5521 agagcccgaa aatcccccat tctaaattcc atccacaatc tgtccactat gaccctcttc
     5581 caatccatcc tgctcggcat tatccaaggt ctgactgaat ttctgcccat ctccagctcc
     5641 gggcacctgg taatcgttcc tcatttgctc ggctgggata tccctggcga tgaagcgttt
     5701 gtgttcaatg tgttggtcca ggttgcaacc atcatcgcgg tgattgccta cttctggaag
     5761 gatctggtga cgattctctc ggctgtgttt tccggccttg ccaggcgaac cctgctcgcc
     5821 aatccgcagg caaggctggg ctggatgatc gtggcggcaa ccatcccggc tgggctggtt
     5881 ggtttgctga tcaaagacct ggtcgagcag gcgttcagca gcctgctagc cacgggcatt
     5941 ttcctgctgg tgacggccgg attactgacc atcgccgagc gtatgggcaa acgccagcgc
     6001 gagctggaca gcttaaagct gagcgacgct gtgtggatcg gtgtggcgca ggcggctgcc
     6061 atcttccccg gtgtatcccg gtcgggggcg acaattaccg ggggaatgct gcgcggcctg
     6121 cagcgccccg cagcagcgcg cttttcattt ctgatgtcaa tcccgatcat gctggccgcc
     6181 gggctgctgg catttctgga tttattggaa atgccgaaca tcggccggct gctgccggta
     6241 ttcatccctg gctttctggc ggcaggtgtc accgggtatt tcgcgattcg ctggctgctg
     6301 ggttacctga cgcgcaagcc cctgacaatc ttctcaattt actgcctggc gttaggcctg
     6361 ctcagcatcg gagtgagcct gtttggttga tcctcatttc aggcggtggc tactgcattc
     6421 cctgttggcc ggtctggcgc tgctgctggc tgcctgccgg ccggcgactt cgctgccgga
     6481 cccgaccgaa tttccagagg ccgtgctagt ccaatacaca ccagcactgc gaatcctcac
     6541 cccggtactg gaagcctgcg ctgccgaaga tcccgacctg gctttgtttc ttgaagaaac
     6601 ccccaaacca gcgatcgacc tatcatccga tcccgatagc cccgttcgcc tggggctggc
     6661 gctgggagag ccattggcgc aaaactattc cgccgccctg acggatgctc aaattgcggt
     6721 gatcacccat ccctccaacc cgatccagga gatcagtgct gccgggcttc aggagctgtt
     6781 tagcgggcaa atccagaact gggctgctat cggcggcagc gaccagaccg tgactccctg
     6841 ggtgctgact ccggcagacg aagcacggca attatttgaa aaagccattc tcggggagag
     6901 taaggtatcc ggccaggccc gcctggcggc ggacccagcc tggatgcttg caagtgtctc
     6961 cactgatccc ggggcggtgg gctatgtacc ggccgcctgg ttgaatgaca gcgtcaacgt
     7021 gcttaagtta gcccagggct tatccgaaga gctgcgcctg cccctgctcg ctctggctgg
     7081 cagcgagccg cagggtgcag cccggagact gctggtctgt ctgcagagcg ggagcgggca
     7141 ggcagccctt aaaatgctct tccaggcgcc ttaatcctcc agtttgcgat tcaaaccgat
     7201 cgcctgcagc accggcacca caaatagaaa acctgaatta ggctggtcca ggtcgccgga
     7261 aatatgctgt acggtagtga tcatcctgtc gaccatctcc tggtctttta ccaccgagag
     7321 cagcatgcga tgaggaattt catcctgctg catgaagtca ctcaggctgg gcagcaaggg
     7381 catatcctca cgcatgccta gcttccgcag atgccccagc ccactgcttt ccaggatggt
     7441 caccccgctc acacccagtg tttcccaggc atctaagatc gccgggcagt cgtctggatc
     7501 atcaacgatc atcacaacca gataactcat ttggagtctc cttcaggact agtttctgcg
     7561 atctgggttt gggattgagg atgaggtttc gcaaataaag accgtaacaa tgggggcgtc
     7621 aataaggtcg tgataattac cacgccgacg atcgcgacca gtgtattttc ggagaccaat
     7681 ccctgggtaa ccccgataga ggcgacaatt aagccaactt ctccccgcga catcatccct
     7741 accccaattt ggagtgcctc catcctgttc agtccgcccc acagagcacc ggctcctgcg
     7801 ccgacgactt tgccaattac agcgaccacg gtcatcccaa taaagagcca gaataccggg
     7861 ccaaataact ggcgggcgtc gactgccaga ccgacattta cgaaaaatat cggcacaaaa
     7921 acgccgtagg ccaggacaga agtgccattg cgaatgcgct cccggaccgg gctgcgagta
     7981 agcgccagac cagcaatgaa agctccggta attgccgcca taccgcctaa cgtttcggcc
     8041 gcgattccat atattaaaac gatgatgaac gtgaaggaca cgacaccctg agcgatatcg
     8101 cgcccggcga ctcgctggct gagcctgggg agcaatttca tacccaggat cgtaccgatc
     8161 cccaggaata atgccatgcg caccacaatc cagaggatat ccagaaaatt cggttcggaa
     8221 gacgtcacgg caataaagat cgatataccc aggattacca ggatatcatc aaacactgcc
     8281 gcccccagca gcgaagtacc gacccgggtg cggagcattt tcagttccat cagggtttgg
     8341 gctgatatgc tcacgctggt agccgccaaa atcagaccga taaaaaaggc ctcctgccca
     8401 ttcatgttga acagggttcc gagaccggct cccaatgcga gtggcaagat gacgcccaac
     8461 gtacctgcca gtgccgacac cttgaatgat ctggttaaat cctccagatg taaatccaga
     8521 ccagcaatga acatcagcaa gagcacgcca atttcagcca gctcgtggat cgtctcactc
     8581 agatggctgc tggtaaatag cggcaggtgc aggaaatcaa tcagcgttgg cccaagcaaa
     8641 atgccaacaa tcaattcacc taaaacggaa ggttgtccaa ggcggatact gatcaaaccg
     8701 ccaatttttg ccagggctat gattcccgcc agagccaggg caagctgtaa gaaattactc
     8761 atcaaaaact ccggtatcct gatccatcga agttgtgcag acacagaatg gatcaggtta
     8821 attccaggct gcaggcagtt aatcttttgc ctgaggtttt atttgtccgt ataaaaacca
     8881 gaacctggat tgagggaaac cgcgctctaa ttcgggatcc cagatgaacg ctcctggagc
     8941 taggagggtt tccagcagtg aatccagcat cctcaccagc tcgtcttcag cagtgggccg
     9001 ctggcgggca tcttcgctga gcagcgtaag ctgcacacgc cgggtgactt cgtaagggta
     9061 gcggtccgca tttgcgtcag tctcgtcgcg cagctccacc ataaaattac gccacagggt
     9121 caggcgcgcc cgaaaattgt gggtggcttt tttctcccag gcaacccggc gtttactgcg
     9181 aacggcttcc aattgattaa cgagctgctg gtattcagcc tggtgtgaat cgggcagaac
     9241 ctgggcctgc agacgtgcgg ctgagaggag caggccatcc aacgtaagct ggggaaaggg
     9301 tggttggcca gccagcggag tagcctggag cggccagtat acttcatcag aatggagata
     9361 ttcgtccaac ccctccagac cggccgcaag gtagcggaga tcgtattcaa tgtctggcat
     9421 agcagcctcc ttggc
//
LOCUS       DATMXK010000553         7297 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_44647, whole genome shotgun sequence.
ACCESSION   DATMXK010000553 DATMXK010000000
VERSION     DATMXK010000553.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 7297)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 7297)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..7297
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_44647"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            191..511
                     /locus_tag="VN363_10060"
     CDS             191..511
                     /locus_tag="VN363_10060"
                     /inference="COORDINATES: protein motif:HMM:NF014716.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aspartyl/glutamyl-tRNA amidotransferase subunit
                     C"
                     /protein_id="HWQ84903.1"
                     /translation="MSDEITRDIFDHIVSLAALELDETEAEYLRQQLNNQLKAIHELE
                     AIPLDDSTPPTSHGVPYTPLIKPGLRADQWIPYPDPEEIIAQAPDSNAGYIIVPEIPH
                     TDLA"
     gene            544..2169
                     /gene="gatA"
                     /locus_tag="VN363_10065"
     CDS             544..2169
                     /gene="gatA"
                     /locus_tag="VN363_10065"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558627.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0030956 - glutamyl-tRNA(Gln)
                     amidotransferase complex [Evidence IEA];
                     GO_function: GO:0050567 - glutaminyl-tRNA synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_process: GO:0006424 - glutamyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatA"
                     /protein_id="HWQ84904.1"
                     /translation="MTEPHMNLSDLPATTISEMIHSGTISARQVTEAALERIAAIDGA
                     PPSLEPNPAAALETDKIHAFITVTAERALERASEIDRQLAAGEDPGPLAGVPFTVKDI
                     FCTRGVLTSAGSRILSNFIAPYTATPVARMEAAGAVMLGKLNMDEFAYGSSSESSAFQ
                     PSPRNPWDTRMVPGGSSGGSTAAVAAGEGVLSIGTDTAGSIRQPAAFCGVVGVKPTYG
                     RVSRYGLIAFGSSLDCPGPVARNIPDAAVMLGVIAGADPHDATTPNVPVPNYLEDLEN
                     GVRGLRIGLSPDYFRITFPDPKTGELQRAPISAEIEAAVLQAAEQLAGLGAEIVENMP
                     MPHTPFGIPAYFVISRVEAASNLHRYDGVKYGYRTTEAVDGLSELYRKTRARGFGLQP
                     KLRILMGMYVSAAQYSEQYYLRAQRVRTLIRADFIQAFDPAGPYHLDALLTPTTPTTA
                     FPIGDVYGDTVLMQYADQLTVPANHAGVPGLSLPCGLDSQGLPIGLQLLGPDFSEATL
                     LQIGRAYELSTARDAWRSLKPAALRSLAGGKQP"
     gene            2166..2990
                     /locus_tag="VN363_10070"
     CDS             2166..2990
                     /locus_tag="VN363_10070"
                     /inference="COORDINATES: protein motif:HMM:NF016816.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="M55 family metallopeptidase"
                     /protein_id="HWQ84905.1"
                     /translation="MKILIAADMEGITGVVNWDQVTSTHSEYSRFRRLMTGDVNAAIE
                     GALAAGASEIIVVDGHSYGYNILIEELNPLARLNSGSGSPLGMVQGIDASIDGVFFVG
                     YHACAGSKNAILDHTWSSATVGGVWLNDMLVGEAGLNAAVCGHFGVPVLMITGDQTVC
                     GEAKALLGSLEVAVVKQASGRMSAECLPPDITREKICEAAARAVHKHRSKQSPPPFSL
                     PAPIQLRVAFFSSDMADRASLLPGVERLNGRELSFSSPDMLTAYREFRAAVSLARG"
     gene            3003..3707
                     /locus_tag="VN363_10075"
     CDS             3003..3707
                     /locus_tag="VN363_10075"
                     /inference="COORDINATES: protein motif:HMM:NF020296.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DNA alkylation repair protein"
                     /protein_id="HWQ84906.1"
                     /translation="MNPTTQAVLSRLQLLANPANVAGMARYGINPDHTLGISIPVLRQ
                     IAKETGRDQALAVELWDTGIHEARLLAGFIADPRQMTEALLDRWVQDFNSWDICDQTC
                     GLFALTPFAYAKAFEWSSHADTFIKRAGFVLMATLAVHDKRASDDHLAQFLPVIARAA
                     SDERNFVKKAVNWALRQIGKRSLSLNELAIATALQIQQQDSKAARWVASDALRELTSE
                     AVQARLETRARQTTTP"
     gene            3736..5172
                     /gene="gatB"
                     /locus_tag="VN363_10080"
     CDS             3736..5172
                     /gene="gatB"
                     /locus_tag="VN363_10080"
                     /inference="COORDINATES: protein motif:HMM:TIGR00133.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016884 - carbon-nitrogen ligase activity,
                     with glutamine as amido-N-donor [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Asp-tRNA(Asn)/Glu-tRNA(Gln) amidotransferase
                     subunit GatB"
                     /protein_id="HWQ84907.1"
                     /translation="MTEFEAIIGLETHIQLNTLTKIFCSCKADSWDDAPNTNICPVCT
                     GMPGVLPVLNRAVVEKAVILAAAMQAEIPPVSFFDRKNYFYPDLPKGYQISQYDRSLA
                     RGGYLDLPMPEGYIRHVTIHKLHIEEDAGKTKNENGQRMIDFNRCGVPLIEMVTAPDL
                     RSADEAAQYLIRLRQLLRWLGISEADMEKGHLRCDANVSIRPVGADYLNPKTEIKNVN
                     SITAVREAIEVEIARQKREVAAGNRIAAWTLEWDEDAGVLRKMRSKETEADYRYFREP
                     DLLSIELDDAWRSEILAGLPELPLARRARFSRQYGLPEYDADILTAERSLSDYFETSV
                     QAYGGDPKRISNWLMNDVLRMLNESDLSAGDLRLTPAYLADIVRLVDAGTINTSTGKA
                     LLEKVQTSGQAPAAIVEQEGLAQVSDRDAIQAVCAQIIAENPGQVTSYRSGKTGLIGW
                     FVGQVMRNSGGKADPVLTRSILEELLSQ"
     gene            5195..6091
                     /locus_tag="VN363_10085"
     CDS             5195..6091
                     /locus_tag="VN363_10085"
                     /inference="COORDINATES: protein motif:HMM:NF024109.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ84908.1"
                     /translation="MVNPQALPNSSYLFLNDLRVHYLRWGVPGGQPVVLLHGLASNAR
                     IWELTAPYLAQAGYDVIAPDQRGHGLTDKPDGDLGFATYTRDLLALITSLELDRPILV
                     GHSWGASVVLEYAARFAIGPRAPAGIILVDGGIIQLNAFPGATWEEMRDRLTPPRLAG
                     MPLEKFLNLLSRPRDSWQPDDQTLQIILGNFEIREDETIAPHLRFEQHMQIVRAMWDS
                     NTYEYFSRLRCPTLLLPAYPAIPIPPDEQDFLSAKEAGLAEIVKSNPKIRYIWMKDSI
                     HDIPLQHPEPLANRMIEFIQTL"
     gene            6140..6595
                     /locus_tag="VN363_10090"
     CDS             6140..6595
                     /locus_tag="VN363_10090"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF025054.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84909.1"
                     /translation="MTDNPEFSLRPARLEDDLTIRRMIRQEHLNPQNLHWPQFTVAID
                     HNGTMIGCGQVKIHSEEVHELASLVVHKEWRGKGVASALINNLIATHPGVLYLTCRAS
                     LEPLYEHFGFVSIPLNVMPPNFRSMYRLINGILALFGQRGGFKVMRRIP"
     gene            complement(6542..>7297)
                     /locus_tag="VN363_10095"
     CDS             complement(6542..>7297)
                     /locus_tag="VN363_10095"
                     /inference="COORDINATES: protein motif:HMM:NF017050.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GMC family oxidoreductase"
                     /protein_id="HWQ84910.1"
                     /translation="SESLLGATSRERTPDYSQGVAITSIFHADPVTTIEPVRYPAGSS
                     LMRLLSAPLIAGGQSIPGRFLKTLGIILTHPLDFLRTHILPGWAQRTTILLVMQSVDN
                     CIRVRLGRSLATLYRRKLVSIADPEHSPPTRIDAGHQITRRFAARINGIPTGSVNEGL
                     LNVPITAHILGGCPMGRDAQEGVVGLDCQVHNYPGLYVVDGSIMPANPGINPSLTIAA
                     LAEYAMSQIPEKGDSISRNTAHDFKAAPLTEQG"
BASE COUNT         1499 a         2250 c         2064 g         1484 t
ORIGIN      
        1 gctgaatcat ctcgtgctcg gcggtgaaag cgaatagtcc actgctgctc atctcttcct
       61 ccggtatgaa tgaatggcgg tttgtttcat tatagcatgc ctgaagaacg tctcagcgaa
      121 tgtgtggtga aacggcaagc agtgttagaa taagagccag ttttgaatac cgcacgcaag
      181 gaaaacgatc atgagtgatg aaatcacgcg ggatatcttt gatcacatcg tcagcctggc
      241 ggcgctggag ctggacgaaa ccgaagcaga gtacctgcgc cagcagctca acaaccagct
      301 gaaagccatc cacgagctgg aagccattcc cctggatgac agcacgccgc ccacctccca
      361 cggggtgccg tacacacccc tcatcaagcc cggattacgc gcagaccaat ggatccctta
      421 tccagaccca gaggaaatta tcgcccaggc ccccgatagc aatgccggtt atatcatcgt
      481 gccggaaatc ccccataccg acctggctta aaccctgcgc atttcaagca accagacctg
      541 aaaatgactg aaccccacat gaatctaagc gacctgcctg caacaacgat cagcgagatg
      601 atccacagcg gcacgatctc cgcccgacag gtaactgaag ccgccctgga gcggattgcc
      661 gccatcgatg gggcgccgcc cagcctggaa cccaacccgg ctgccgctct tgagacggac
      721 aagatccacg cttttatcac cgtcaccgcc gagcgcgccc tggagcgcgc cagcgagatc
      781 gatcgccagc tggcagctgg cgaggacccc ggacctctgg caggagtgcc tttcacagtg
      841 aaagacatct tctgcacccg cggggtgctc accagcgctg gctcacgcat cctgagcaac
      901 tttattgccc cgtatacagc gacccccgtg gcgcgcatgg aggcagccgg cgcggtgatg
      961 ctgggcaagc tcaacatgga cgagtttgcc tatggctcat ccagcgaatc atccgccttc
     1021 cagccctccc cgcgcaatcc ctgggatacc cggatggtcc cgggcggctc atccggcggc
     1081 agcaccgccg cagttgctgc cggagaaggc gttctttcga tcggcacgga tactgcgggg
     1141 tcgatccgcc agccggctgc cttctgcggg gtcgtagggg tcaagcccac ctatgggcgt
     1201 gtttcgcgct acggcttgat cgctttcgga tcctcgctgg attgtcccgg ccccgtggcg
     1261 cgtaatattc cggatgccgc agtgatgctg ggtgtgattg ccggggcaga cccgcatgac
     1321 gcaaccaccc caaacgtgcc ggtgcccaat tatctggagg acctggaaaa tggggtgcgc
     1381 ggcctgcgca tcggtctatc gccagattat tttcgcatca ctttccccga tcccaaaaca
     1441 ggcgagctgc agcgggctcc aatctcggct gagattgagg ctgcggtcct gcaagctgct
     1501 gagcagctgg ccggcctggg cgctgagatc gtcgaaaata tgcccatgcc gcataccccc
     1561 tttggcatcc cggcgtattt cgtgatctcc cgcgtcgaag ctgcctccaa cctgcaccgc
     1621 tacgatgggg ttaaatacgg ctaccggact accgaagcag tggatgggct gtctgagctt
     1681 taccgcaaaa cccgtgccag gggttttgga ctgcagccca agctgcgcat cctgatgggg
     1741 atgtatgtca gtgctgccca gtacagcgag cagtattacc tccgcgccca gcgcgtgcgc
     1801 accctgatcc gggctgactt catccaggca ttcgatccgg ctggtcctta ccacctggat
     1861 gccctgctca cacccaccac gccaacgact gcattcccga ttggggatgt ctacggcgac
     1921 actgtgctca tgcagtacgc cgatcaactc accgtacctg ccaaccacgc cggcgtcccc
     1981 ggtctctccc tgccctgcgg gctggattct cagggtctgc ccatcgggct gcagctgctg
     2041 ggtcccgatt tctcggaagc aaccctgctg cagattgggc gcgcttatga gctttcaact
     2101 gcccgggatg cctggcggtc gctcaagccg gctgcgctgc gcagcctggc tggaggcaaa
     2161 cagccatgaa aatcttgatt gctgcggata tggaaggcat caccggggtg gtcaattggg
     2221 accaggtcac ctcaacccac agcgagtaca gccgttttcg ccgcctgatg actggggatg
     2281 tgaatgccgc cattgaaggc gccctggcag ccggtgccag cgagattatt gttgtggacg
     2341 gacattccta cggctataac atcttgatcg aagagcttaa cccgcttgcc cgcctgaaca
     2401 gcggcagcgg cagtcccctc gggatggtcc agggcatcga tgccagcatc gacggcgtat
     2461 ttttcgtcgg ctatcacgcc tgcgcaggct cgaagaatgc catcctcgac catacctggt
     2521 ccagcgccac agttggcggt gtgtggctga acgacatgct ggtcggggaa gccggattga
     2581 atgcagcggt ctgcgggcat tttggcgttc ccgtgttgat gatcactggc gaccagaccg
     2641 tttgcggcga ggcaaaagcc ctgctcggca gcctggaagt tgccgtggtc aaacaagcca
     2701 gcgggcgcat gtctgctgag tgtctgccgc cggatataac ccgtgagaag atctgcgaag
     2761 ccgccgcccg ggcagttcat aagcatcgca gcaagcagtc ccccccgcct tttagcctgc
     2821 ctgcaccgat ccagctccgg gtggcctttt tcagctcgga tatggcggac cgtgccagcc
     2881 tgctccctgg cgtggagcgc ctgaacgggc gcgagctcag cttctccagc ccggatatgc
     2941 tgaccgcata ccgggaattc cgcgccgcgg tcagcctggc tcgcggctga gacagaccat
     3001 aaatgaaccc tacgacgcag gctgtcctct cccgcctcca actgcttgcc aatcctgcta
     3061 acgtggccgg gatggcgcgc tacggcatca accctgacca tacgctggga atctccatcc
     3121 cggtgctgcg ccagatcgcc aaagaaacag gccgcgacca ggccctggct gttgagctgt
     3181 gggataccgg catccacgaa gcccgcttgc tggctggatt tatcgctgac ccgcgccaga
     3241 tgaccgaagc gctgctcgat cgttgggtgc aggatttcaa ttcctgggat atttgcgacc
     3301 agacctgcgg attgtttgcc cttactccct tcgcctatgc gaaggctttt gaatggagct
     3361 cgcacgcgga tacttttatc aaacgcgccg gctttgtgct catggcaacc ctggcggtcc
     3421 atgacaaacg tgcctcggat gatcacctgg cgcagttctt gccggtcatc gcccgggcag
     3481 ccagtgacga gcgcaatttc gtcaagaaag ccgtgaattg ggcattacgc cagattggca
     3541 agcgcagtct cagtttgaat gagctggcga tcgccaccgc cctgcagatc caacaacagg
     3601 acagcaaagc agcccgctgg gtcgcatccg atgcgctgcg tgaattgacc agcgaagccg
     3661 tccaggctcg cttggagacg agagccagac agactacaac cccataacag tcttcgccaa
     3721 cgatcgaggc attgaatgac cgaatttgaa gctatcatcg ggttagaaac ccatatccag
     3781 ctcaacaccc tcaccaagat tttctgcagc tgcaaagcgg attcgtggga cgacgccccc
     3841 aacaccaata tctgcccggt ctgcacaggt atgccaggcg tgctgccggt cctgaatcgc
     3901 gccgtggtcg aaaaagctgt cattctggca gccgccatgc aagccgagat cccgccggta
     3961 tcattcttcg accgcaaaaa ctatttttac cccgacctgc ccaaaggcta ccagatctcc
     4021 cagtatgacc gctcgctggc aaggggcggc tatctggatt tacctatgcc agaggggtac
     4081 atccggcatg tcacgatcca caagctgcat atcgaagaag acgccggaaa gactaaaaac
     4141 gaaaatggac agcggatgat cgattttaac cgctgcgggg tgccgttgat cgagatggtg
     4201 accgcaccgg atctgcgttc ggcagacgag gctgcccagt atttgatccg cctgcgccag
     4261 ctgctgcgct ggctggggat ttctgaagcg gatatggaaa agggccacct gcgctgtgat
     4321 gccaatgtgt ccatccgccc ggtcggagcc gattacctga acccgaagac cgagatcaag
     4381 aatgtgaact cgatcactgc ggtccgggaa gctatcgaag tagagattgc tcgccagaaa
     4441 cgcgaggtcg ccgccggcaa tcgcattgct gcctggactc tggaatggga tgaggatgcc
     4501 ggggtgctgc gtaagatgcg ttccaaggaa accgaggccg attaccgtta tttccgcgag
     4561 cccgacctgc tttcaatcga gctggatgac gcctggcgtt ccgagatcct ggccggtctg
     4621 cccgagctgc ctctggcccg gcgggcgcgt ttttcccgcc agtatggcct gcctgagtat
     4681 gacgccgata tcctgaccgc cgagcgcagc ctgtccgatt atttcgagac cagcgtccag
     4741 gcttatggcg gcgatcccaa gcgcatctcc aactggctga tgaacgatgt gctgcgcatg
     4801 ctcaatgaaa gcgatctctc ggctggtgac ctgcgcctga caccggccta tctggcggat
     4861 atcgttcgcc tggtcgacgc cgggacaatt aacacttcta ccggtaaagc cctgctcgaa
     4921 aaggtccaga ccagcggcca ggcgcctgcc gccattgtag agcaggaagg actggctcag
     4981 gtcagtgacc gggatgccat ccaggcggtc tgcgcccaga tcatcgccga gaatcccggg
     5041 caggttacca gctaccggag cggaaagacc ggactgatcg gctggtttgt aggccaggtg
     5101 atgcgtaatt caggcggtaa agctgacccg gtgctaacgc gcagcatcct ggaagaatta
     5161 ttgagccaat agtcgttcgg cgaggtactc ttttatggta aatcctcaag cgcttcccaa
     5221 ctcctcttat cttttcctca acgacctgcg cgttcattac ctgcgctggg gtgtgccggg
     5281 tggacaaccg gtcgtactgc tccacgggct ggcatcgaat gcccgcatct gggagctgac
     5341 tgccccttac ctggcgcaag ccggctacga tgtgatcgcc ccggaccagc gcggccacgg
     5401 cctgacggat aaaccggatg gcgacctggg ttttgcgacg tatacccgcg atctcctggc
     5461 cctgatcacc agcctggagc tggaccgccc gatcctggta ggtcattcct ggggcgcctc
     5521 ggtcgtcctg gaatacgccg ctcgctttgc catcggaccc cgtgccccgg ccgggatcat
     5581 cctggtagat ggtgggatca tccagctcaa tgcctttccc ggcgccacct gggaagagat
     5641 gcgcgaccgc ctgactcctc caagattggc gggcatgccg ttggagaaat tcctcaacct
     5701 gctttcccgg ccacgggaca gctggcagcc cgatgatcag acgctccaga ttatcctggg
     5761 caattttgag atccgtgagg atgaaaccat tgccccccac ctgcggttcg agcagcatat
     5821 gcaaattgtg cgcgccatgt gggattccaa tacctacgag tatttttctc gcctgcgctg
     5881 ccccacttta ttactgccag cctatccagc catacccatt ccacccgatg aacaggattt
     5941 tctgtcagcc aaagaagccg gactggctga aatcgtgaaa tccaacccaa agattcgtta
     6001 tatctggatg aaagacagca tccacgatat tcccttgcag catcctgagc ctctggcgaa
     6061 ccggatgatc gagtttattc aaaccttata ggtgatttgg atcgagccct agtggcctgt
     6121 ctgaagcttg cttaatcaca tgaccgacaa tcctgaattt agcctgcgcc cggcgcgttt
     6181 agaagatgat ctgaccatcc ggcgcatgat ccgccaggaa catcttaacc cccagaatct
     6241 tcactggcca caatttactg tggccatcga tcacaacggg actatgatcg gctgcggaca
     6301 ggttaagatc cattccgagg aggtgcatga gcttgcttcc ctggtggttc acaaagagtg
     6361 gcggggtaag ggtgtggcca gcgcgttgat caacaacctg atcgccacgc accccggcgt
     6421 gctctacctg acctgccggg ccagcctgga accactttac gagcactttg gctttgtctc
     6481 aatccccctg aacgtaatgc cacccaactt ccggtcgatg tatcgcctga tcaatggaat
     6541 tttagccctg ttcggtcagc ggggcggctt taaagtcatg cgccgtattc cttgagatcg
     6601 aatcgccttt ttccggtatc tggctcattg cgtattcggc caaagccgcg atcgtcaggc
     6661 tggggttaat ccccggattg gcgggcatga tcgagccatc caccacatac aaacccgggt
     6721 agttatgaac ctggcagtcc agccctacca ccccttcctg cgcatcccgc cccatcgggc
     6781 agccccccag gatatgagct gtgatcggca catttagcaa gccttcgttg accgagccgg
     6841 tcggaatccc attgatcctt gctgcaaaac ggcgggttat ttgatgcccg gcatcgatcc
     6901 tggtaggtgg gctgtgctcg gggtccgcta tcgataccag cttccggcga tacaaagtcg
     6961 ccaggctgcg tcccagccgg acgcggatgc agttgtccac cgactgcatt accaacagga
     7021 tcgtggtccg ctgcgcccag cccggcagga tatgggtgcg taagaaatcg agcggatggg
     7081 tgaggatgat ccccagggtt ttcaagaacc ggcccgggat gctctgcccg ccggcaatca
     7141 acggcgctga gagcagacgc atcagtgaag agcctgccgg gtagcgtacc ggctcgatgg
     7201 tcgtcaccgg atcggcgtgg aaaatagacg tgatggcgac gccctgagaa taatctggcg
     7261 ttcgctcacg actggtcgcg cccaacagcg attctga
//
LOCUS       DATMXK010000554         2883 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_120055, whole genome shotgun sequence.
ACCESSION   DATMXK010000554 DATMXK010000000
VERSION     DATMXK010000554.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2883)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2883)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2883
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120055"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VN363_10100"
     CDS             complement(<1..578)
                     /locus_tag="VN363_10100"
                     /inference="COORDINATES: protein motif:HMM:NF019317.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84911.1"
                     /translation="MSIISVEQKKQPRIFYILLLVTGTLVMVAAMILPSLERLSLPSL
                     QVGQVASQDYLAYESTSYTSQVLTEQKQEAASRAVAPVYTRPDPSIARRQLEKLYVNL
                     AFISSVRADSFGTLEQKLADLSALEDIHLDQEMGTRILALSEARWGAVQQETIAVLEQ
                     LMRDVIRPDRLDDSRSSALSLVSLSFPENQAE"
     gene            complement(583..1014)
                     /locus_tag="VN363_10105"
     CDS             complement(583..1014)
                     /locus_tag="VN363_10105"
                     /inference="COORDINATES: protein motif:HMM:NF020977.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="GatB/YqeY domain-containing protein"
                     /protein_id="HWQ84912.1"
                     /translation="MQLETALKDAMRAQDDLRKRTLRMALSAIHLAEIESKQEQDDPA
                     VMAALQKEIKTRQESVEEAKKANRPELAAATQAEIEVLQSFLPQPFSPAELEALVREA
                     VAEVGATSAREIGPVMKILMPRLQGRATGSEANQVLRRLLG"
     gene            complement(1141..2385)
                     /locus_tag="VN363_10110"
     CDS             complement(1141..2385)
                     /locus_tag="VN363_10110"
                     /inference="COORDINATES: protein motif:HMM:TIGR00619.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="exonuclease SbcCD subunit D"
                     /protein_id="HWQ84913.1"
                     /translation="MPTILHFADAHIDMANYGRHDPQSGLPLRVLDFLKSLDTIVDAA
                     IAERVDLVLFAGDAYKDRSPAPTYQREWGKRIMRLSRAGIPTLLLVGNHDLSPAMGRA
                     HALDPFTTLEVPNVRVIDKPMFLGREQLAGLGLQVLALPWIGRSGLMANLNLQGETVS
                     QVNDRIQDRLGELIASWLEEADPSLPVVLAAHATVQGAQYGGERMVMLGSDLALPPSL
                     VRNPRLDYVALGHIHKAQNLNPDAHPPVIYPGSIERVDFGEVADDKFYVLAHVERGKT
                     TVEWRKLTGVRPFIDIKHLIKDANDLMDKTLAAFPSSSRLEGAIFRMVIEYPRELEPL
                     IDENALRQAAENTFEFHLVKRPLSETRVRLPEGHALSELNPLELLDQYWAASHVDDEE
                     QKALHNLAAEVIGSTGQGEEEN"
     gene            complement(2390..>2883)
                     /locus_tag="VN363_10115"
     CDS             complement(2390..>2883)
                     /locus_tag="VN363_10115"
                     /inference="COORDINATES: protein motif:HMM:NF019339.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="tetratricopeptide repeat protein"
                     /protein_id="HWQ84914.1"
                     /translation="GAQWDETANYQYAAELASTEIFNSTGRDLYFAWFNRGTNLMYLQ
                     DYGGAAAAFDEAFRLYPEIPEKSRPWRMMWYQTGPYFAYFFTQRYYDVIALATTTIET
                     ATEPSIEESFYWRARAFYALYELEGKPEYLAHAEEDLRASLEWHPNFGPTLGQMQVLG
                     MVP"
BASE COUNT          572 a          896 c          780 g          635 t
ORIGIN      
        1 atctctgcct ggttttctgg aaacgacagg ctgaccaggg aaagggcgct gctgcgagaa
       61 tcatccagac ggtccgggcg gatcacatcg cgcatcaatt gttccagcac ggcaatcgtt
      121 tcctgctgga cagcccccca gcgcgcctcg cttaatgcca ggatgcgagt tcccatctcc
      181 tggtcgagat ggatatcctc cagagctgaa agatcagcca gcttttgttc cagggtaccg
      241 aaggaatctg cccgcacact ggagataaac gccaggttta catacagctt ttccaactgc
      301 cggcgagcaa tgctggggtc ggggcgcgta tataccggcg caacagcccg gctggcagcc
      361 tcttgtttct gttccgtcag tacctggctg gtgtagctgg tgctctcata cgccaggtaa
      421 tcctgggacg caacctggcc aacctgcagg gaaggcagtg ataaccgctc caatgacggg
      481 aggatcatcg ctgcaaccat caccagggtg ccggtcacca gcagcaggat ataaaaaatc
      541 cgcggctgct tcttttgctc aaccgatata atactcatgc acttaaccca acaggcggcg
      601 cagaacctgg ttggcctcgc tccccgttgc ccgcccttgc aggcgcggca ttaatatttt
      661 catcaccggg ccaatttcac gcgccgaggt cgcccccact tcggcaactg cttcgcgtac
      721 cagcgcctcc agctccgccg ggctgaatgg ctgcggcagg aaactctgca ggacttcgat
      781 ttcagcctgg gtggcggctg ccagctcagg ccggttggct ttcttcgcct cttcgaccga
      841 ttcctggcga gtctttattt ccttttgcaa ggctgccatc actgccgggt catcctgttc
      901 ctgtttggat tcgatctccg ccagatgaat tgccgacagc gccatacgca gggtgcgctt
      961 gcgcaggtcg tcctgggcgc gcatggcgtc ctttaatgca gtttcgagtt gcattttcaa
     1021 gctcataagt tcattatacc ccgtacgctc ttcaatttag ctcaggctgg attgtggcag
     1081 gtgctgctca ttggcctgta actcgcttct acaagcgcag ctcgccagcc tacagctacc
     1141 ttaattctct tcttcaccct gcccggtgct gccgatcact tcagcagcca gattgtgcag
     1201 cgctttctgt tcttcatcat ctacatggct ggcagcccaa tactggtcca gaagctccag
     1261 cgggttcagc tcgctcagcg cgtgcccctc gggcaggcgc acccgggttt cgctcaacgg
     1321 gcgtttaacc agatgaaatt cgaaggtgtt ttcagccgcc tggcgcagcg cattctcgtc
     1381 gatcaagggc tccagctcgc gcgggtactc aatcaccatt cggaagatcg cgccttccag
     1441 cctggatgag gatgggaacg ccgccagggt cttatccatc aggtcgttgg catccttaat
     1501 tagatgctta atgtcgataa aaggccgcac gccggttaat tttcgccact cgaccgtggt
     1561 cttgccgcgc tccacatgcg ccaggacgta gaatttatcg tcggcaacct ccccaaagtc
     1621 aacccgctcg attgacccgg ggtaaatcac cggcgggtga gcatctggat tcaggttttg
     1681 cgctttatgg atatgcccca acgccacata gtccagccgg ggattgcgca ccagcgaggg
     1741 aggtaaagcc aggtctgagc cgagcatcac catgcgttcc ccaccgtact gcgctccctg
     1801 cacggttgca tgcgccgcca gcaccaccgg caggctgggg tctgcttctt ccagccagct
     1861 ggcaatcagc tcacccagcc ggtcctggat cctgtcattg acctggctga ccgtttcgcc
     1921 ctgcaggttt aaatttgcca tcaagcccga gcgcccaatc cagggcagcg ccagcacctg
     1981 caggcccagg ccagcaagct gctcgcgccc caaaaacatc ggtttgtcga tcacccggac
     2041 gttcggtacc tccagggtgg taaacggatc cagggcatgc gcgcgcccca tggcgggtga
     2101 gagatcatgg ttgcccacca gcagcagggt tgggatgcct gcccgtgaca ggcgcattat
     2161 ccgtttaccc cattcgcgct ggtatgttgg ggcagggctg cgatctttat acgcgtcccc
     2221 ggcaaacaat accagatcca ctcgctccgc gatcgccgcg tcgacaatcg tatccagcga
     2281 tttcaggaaa tccagcaccc gcagcggcag cccgctttgg ggatcatggc gaccgtaatt
     2341 cgccatatcg atatgtgcgt cggcaaaatg gaggattgtt ggcatagaac taaggcacca
     2401 tccctaagac ttgcatctgc cccaatgtcg gcccaaaatt ggggtgccat tccaggctgg
     2461 cgcgcaggtc ttcctcggca tgcgccagat actctggctt cccctccagc tcatacagag
     2521 cataaaaagc ccgcgcccgc cagtagaagc tttcttcgat cgacggctca gtggcggttt
     2581 cgatcgtggt ggtcgccagg gcaattacat cataataacg ctgggtgaaa aaataagcga
     2641 agtacggtcc ggtctggtac cacatcatcc gccagggccg agatttctcg gggatctctg
     2701 ggtacagcct gaaagcctcg tcgaacgccg ccgccgcccc cccgtaatcc tgcaggtaca
     2761 tcaggttggt gccgcgattg aaccaggcga aatacaggtc ccgcccggtg gagttaaaga
     2821 tctcggttga ggcaagttca gctgcgtact ggtaatttgc cgtctcgtcc cactgtgcac
     2881 cca
//
LOCUS       DATMXK010000555        11556 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_166946, whole genome shotgun sequence.
ACCESSION   DATMXK010000555 DATMXK010000000
VERSION     DATMXK010000555.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 11556)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 11556)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..11556
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166946"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            24..461
                     /locus_tag="VN363_10120"
     CDS             24..461
                     /locus_tag="VN363_10120"
                     /inference="COORDINATES: protein motif:HMM:NF024854.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MarR family transcriptional regulator"
                     /protein_id="HWQ84915.1"
                     /translation="MMIKTSENLPMGRLLANLGRTHGMRIDQLMEQIGLYRGQAILLL
                     TLAEQNGLTHGEIAERLCISPAAASKVIKRMEGLGYVQRTTDPTDERVSRVYLLDGGR
                     AKLDDIHAVFHAINARMLKDFSPEEHAALHRMLLRMTHNLQNS"
     gene            484..2223
                     /locus_tag="VN363_10125"
     CDS             484..2223
                     /locus_tag="VN363_10125"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84916.1"
                     /translation="MIKKLAPFIGQYKKYALVVPLLVFLDVLAELSMPLLMARIIDVG
                     IPSMDIGYIARTGGYMVLLALAGILFGVLFMYSAAVAAMGFGANLRNALFEKVQAFSF
                     NNIDRFSTASLITRLTNDANNVQMTLMMMLRILIRAPMMLVVAFFLAYSINARLSLVL
                     AVAIPILTVGVAFIMNSAVKRFSVVQGKIDAINNVLQENLIGVRAVKSFVRQDFEIGK
                     FKAANDGLTNAAIRAVSIVILNMPLMMLVMNGATLAVIWQGGRLVGSGEMGAGGLVSF
                     ISYIMQILMSVMMLSMVVVMSARAKASAERIVEVLDTPLDILDKPQTTTPAVRAGKVE
                     FRDVDFKYDLTGSGENVLSGISFTAEPGQVIGIVGGTGSGKTTLVTLIPRLYDATAGA
                     VLVDGVDVRDYPLETLRQEIGVVLQKNTLFSGTIRDNLRWGNPDATQAELETASRAAQ
                     AHDFILDFPDGYDTLLGQGGVNLSGGQKQRLSIARAMLKKPKILILDDSTSAVDSTTE
                     SRIRQAFQQELHATTVFIIAQRISSVHEADQILVIEDGRIAGQGTHAELLAGNRIYQE
                     IDASQQEGVLANV"
     gene            2216..4279
                     /locus_tag="VN363_10130"
     CDS             2216..4279
                     /locus_tag="VN363_10130"
                     /inference="COORDINATES: protein motif:HMM:NF012869.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0042626 - ATPase-coupled transmembrane
                     transporter activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter ATP-binding protein"
                     /protein_id="HWQ84917.1"
                     /translation="MSEPRRTPSLTNIPAASARPGGPRHGGGHGAHFERPKDARKTLR
                     RLLAYLKGKELLLVIVLGLMLLSTGSMLAGNYFLKPLINDYILPGDFPGLAKALVVLG
                     AIYLVGALASYLQSRLMMVTALRTTNTLRRDLFARLQKLPLKYYDTHAHGDVMSRFTN
                     DIDNVQVALEQSLVQLLSSALTFTGAVVLMIVLSPILFLVTFLMLGLMIFLIGKITKR
                     SSQFFKAQQANLGNVNGYIEEMVDGLKVVKVFTREQEAIAEFKLRNEAYRQSATSANF
                     TAGAVMPIMGNLNNIAYAGTAMFGGILAVVSGFDIGSLAAFLQYTRNVGMPVQQITNQ
                     LNVILAAMAGAERVFEVMDEAEETDSGTVTLVEATRQADGKLVAGRNGKHPDLQADPG
                     VPTRSWATQESGATQEIWAAQKSGAAQKSGVTQKSWAWKVPNGNNDFHYVELQGDVRF
                     LDVTFGYEENKTVLHDLSLYAKPGQKIALVGSTGAGKTTITNLINRFYDIDEGQITYD
                     GIDVKQIRKDDLRNSLGMVLQDTHLFSGTIMDNIRYGRLDATDEECVRAAKQVSAHSF
                     IKRLPEGYQTLISADGGSLSQGQRQLLAIARAAVADPPVMILDEATSSIDTRTERLIE
                     KGMDALMKDRTVFVIAHRLSTVRNSDAILVIEDGRIIERGDHEDLLEQKGRYYQLYTG
                     QYQLS"
     gene            complement(4424..4966)
                     /locus_tag="VN363_10135"
     CDS             complement(4424..4966)
                     /locus_tag="VN363_10135"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84918.1"
                     /translation="MRTPYPQSQSLTAESFVSYEIDSTAGLGILTLNQCIFNEVYTDT
                     LCAFFQEVKDQGIQNIAVDLRRNGSGNSKVVNEFLRYLDVDQAAISGGANIRFGPYLY
                     QTSSEGEPNERIPELTYTGKIYVLTAVQTSAQPPILPPPSPIIAWASSSVRLPAICPL
                     PMAMCSDSNPTCRAGIQSLP"
     gene            5241..5795
                     /locus_tag="VN363_10140"
     CDS             5241..5795
                     /locus_tag="VN363_10140"
                     /inference="COORDINATES: protein motif:HMM:NF013630.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="LysM peptidoglycan-binding domain-containing
                     protein"
                     /protein_id="HWQ84919.1"
                     /translation="MLKTISSRTIWIALSLVLVLSMLAVAAPQPVQAPTDATCAKTVT
                     VKEGQTLRRIAKQNDVRLWQLARANDIDDPYAPLEVGDKLCIPGEVTFSSKSYWTATF
                     NTSGEVKIIGQGFKKQAPFFLKVRENADTKQFKLAKLVTTSTGTFKNTEYMVPKDLRD
                     NPYLFVCLKDGITDAVQCKQIWRY"
     gene            complement(5871..8723)
                     /locus_tag="VN363_10145"
     CDS             complement(5871..8723)
                     /locus_tag="VN363_10145"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="PKD domain-containing protein"
                     /protein_id="HWQ84920.1"
                     /translation="MKNYPRLLSKINRNLLWQGLSCLLVLALLLAASSVSAQETTPGV
                     WSYLGGQPGANLGSTLASAGDVNGDGYADILLGAYLYESSNFLQAEGTAYLFYGSAAG
                     LGPQPGWAKNGGTSFAYYGWAVASAGMLNGDVYSDVAVSSVICTANDKSAGAVDVFQG
                     SAAGLSLTATPRLISSQANACFGYALAKAGDVNGDGFGDFLIGAPYYDDEFVDEGAVY
                     LYYGSASGLSTTPARTFYGGQANARLGTALTAGNLNGDPHSDIVLAAPGWSNGQSGEG
                     QVTIYFGGPGGPSAVPDWTNEINQIGAQYGSSVAVAGDINGGGIDDLLVGADHFDGTL
                     GDEGKVFLYYGAASGPSTTPDWSFSSGNLDAHLGNSVASAGDINGDSFADLILGAWRY
                     TDPEAVEGAVLVFFGAAAGPAAIPDWTHQLNQPNANLGWAVAGAGDINNDNFGDIIVG
                     APGYESGSGADEGLAKVFFGEGTGLSEPPLSYTLTPNPANEGSSVTLLGSFTVADPLA
                     NFTLTVSWGDGSPSTVVALGTGRFFDEQHTYSDNGLFSVEISVSDGATSLGDVAVLTV
                     NNVVPTVALTGAASVVEGASYTLTIGPKVDPGADTLSACQLDWGDGSPLQDCLGALGG
                     TRTHVFGDGPASHTVRIHLTDEDGNYQDVDTLPVSVTNASPVAVNDAYSVPFNQTAVI
                     SAPGVLENDSDVPADTLTAQIVSDVTVGTLALQASGGFTYTPPTNFSGTATFTYQVQD
                     DDGATSAVATVTLTVSSNIPIVAHAGPDQTVNEGAAVQFAGSFEDPDLPGPLPPGNIS
                     WDFGDGTPELTGSLTPVHTYADNGIYTATLTLDDGQGAVDSDTVLITVLNVAPVVNLG
                     TDTYIDLGAVFTRQGTFTDPGADTWNATVNYGDGSGSQPLALTGQAFSLSHAYPNAGT
                     YLITVTVADDDGGVGTASINLLVGDHYLYVPLVQNP"
     gene            complement(8962..9162)
                     /locus_tag="VN363_10150"
     CDS             complement(8962..9162)
                     /locus_tag="VN363_10150"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84921.1"
                     /translation="MSDEKHYRDGDLDTHAAFLAAQPTPLIWKFITAMAERLRPQETA
                     VAQDPEQARPGAPAIYKSVRLR"
     gene            complement(9219..9290)
                     /locus_tag="VN363_10155"
     tRNA            complement(9219..9290)
                     /locus_tag="VN363_10155"
                     /product="tRNA-Arg"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(9255..9257),aa:Arg,seq:ccg)
     gene            9414..>11556
                     /locus_tag="VN363_10160"
     CDS             9414..>11556
                     /locus_tag="VN363_10160"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84922.1"
                     /translation="MPNRRQSIPRILILLAGVCLAAWLSACTATPPPLSPGDWLVEDL
                     LALDPPENRLANDPLTRADLIAAYARPTLDAIHLRVDLLDFPSGQDADLYIAIDSGSR
                     GTNILPLAGTSELSWEVLLVIPAIGELQVLDSDLNPIPDSALRVARSPGSHSIALAFN
                     PASLPGAAPSLRLEFFTALPGSQEIADRLGPQTPQTAIPQAMPALLAFWDSFPAATPA
                     QALRRWDGAHTGPLGGRHGLFNLLRTARAREIPLVLLDLGSPETLAALEYAGGMGLTA
                     ELALSRQLILPASLPGLLAQAGEVLPLVDAELLAAWLEASRESQLAYGLPASPFVSAP
                     LSAAQFSGLEASLQTSAGRTIFTFRAGSSGPFGYAARRGQQRLLILPQLPAGSTPDRL
                     DFALEARRELAQAAWDWNAAETDPGQFLTLGGSLPESPFGSPAYARLAFQYLQEHPWI
                     RLLSEHDLRTLPASRHPLAVTSSPIPPPTSDLQAQAVQAAPEAALELPQPAVIALPVS
                     RWGADFLRAIYTQPYPAPPGLASLRAGYLGMLSSLAEAEAWLNNPTPRLECSGDPDQE
                     GAAECILADESFYAQIELDDGRLELGIARRDGSAREAGGAHLLIAPGYLLVSGLGDPA
                     GWNLAAGERADPQVLAGGFGGGEGPYTVEILADGLRLSSPDGTTIKEYRLENGGLAFH
                     IAGAPGSLWQLPLVIEPQQRQMPGWWKESAFM"
BASE COUNT         2406 a         3462 c         3487 g         2201 t
ORIGIN      
        1 ccagttaacg ttcaacaggt gaaatgatga tcaaaacgtc cgaaaatttg cccatgggcc
       61 ggctgctggc caatctgggc cgcacccatg gcatgcggat cgaccagctc atggagcaga
      121 tcgggctgta tcgcggccag gcgatcctgc tcctgaccct ggcagagcaa aatggcctga
      181 cccacggcga gatcgccgag cggctgtgca tctcgccggc tgcagccagc aaggtgatca
      241 agcgcatgga agggctgggc tacgtgcagc ggaccaccga tcccacggat gagcgcgttt
      301 cccgggttta cctgctggat ggcgggcgag caaaactgga tgatatccac gcagtcttcc
      361 atgcgatcaa tgcccgcatg ctgaaggatt tctcacccga ggagcacgcc gcgctgcaca
      421 ggatgctgct ccggatgacc cataatttgc aaaattcgta acttatcgat ttggagaatc
      481 gcgttgataa aaaaattagc gccctttatc gggcaataca agaaatatgc attggtcgtg
      541 ccgctgctgg ttttcctgga tgtactggca gagctgagca tgccgctgct gatggccagg
      601 ataatcgatg tgggcatccc cagtatggac atcggatata tcgccagaac cggcgggtat
      661 atggtgttgc tggcgctggc ggggatttta tttggggtgc tgttcatgta ctcggctgcg
      721 gttgcagcca tgggttttgg ggcgaacctg cgcaatgccc tgttcgaaaa ggtgcaggcg
      781 ttctcattca acaatatcga ccgtttcagc actgcctcgc tgatcacccg cctgaccaat
      841 gatgcgaaca acgtccagat gaccttaatg atgatgctgc gcatcctgat acgcgccccg
      901 atgatgctgg tggtggcgtt cttcctggct tacagcatca acgcccggct ctcgctggtg
      961 ctggcggtgg cgatcccgat cctgacggtc ggggtggcat tcatcatgaa ctcggctgtc
     1021 aagcgatttt ccgtggtgca aggaaagatc gacgccatca acaatgtact gcaggaaaat
     1081 ctgatcgggg tgcgcgcggt caaatcgttt gtgcgccagg attttgagat cggtaaattc
     1141 aaagctgcca acgatggact gaccaacgct gccatccggg cggtgagcat tgttattttg
     1201 aatatgccac tgatgatgct ggtgatgaat ggggcgaccc tggcggtgat ctggcagggc
     1261 gggcgcctgg ttggaagcgg cgaaatggga gccggcgggc tggtcagctt catcagttat
     1321 attatgcaga tcctgatgag cgtgatgatg ctctcgatgg tcgtggtgat gagcgcccgg
     1381 gcgaaagcca gcgccgagcg catcgtggaa gtgctggata ccccgctgga tatcctggac
     1441 aagccgcaga caaccacccc cgccgtccgc gccgggaagg tggaattccg ggatgtggac
     1501 ttcaaatacg accttaccgg cagcggcgag aacgtgctca gcggaatcag ctttaccgcc
     1561 gagccggggc aggtgatcgg catagtaggt ggcaccggct ctggcaagac caccctggtt
     1621 accctgatcc cacgcctgta cgacgcgact gccggggcgg tgctggtgga tggggtggat
     1681 gtgcgcgatt acccgctgga gacgctgcgc caggagatcg gggtggtgct gcagaagaac
     1741 actctgtttt cgggcacgat ccgcgacaat ctgcgctggg ggaacccgga tgccacccag
     1801 gcagaactgg aaacggccag ccgcgctgcc caggcgcacg attttatcct ggacttcccg
     1861 gatggatacg ataccctgct ggggcagggc ggcgtgaacc tgtctggcgg gcagaaacag
     1921 cgcctgagca ttgcccgggc gatgctcaag aaacccaaaa tattgatcct ggatgacagc
     1981 accagcgcgg tcgactccac caccgagagc aggatccgcc aggcattcca acaggagctg
     2041 cacgctacca cggtgttcat cattgcccag cggatcagct cggttcacga agccgatcag
     2101 atcctggtga tcgaagacgg ccggatcgcc gggcagggca cgcacgcaga gctgctggcg
     2161 ggaaaccgca tctatcaaga aatcgatgcc tcacagcagg aaggagtgct cgccaatgtc
     2221 tgagccgcgc cgcacccctt cgctgaccaa tatccccgcc gcctctgccc gcccgggtgg
     2281 gccgcgacac ggcggagggc atggcgccca tttcgagcgc cccaaggatg cccgcaagac
     2341 cctgcgccgc ctgctggctt acttgaaagg caaggagctg ctgctggtga ttgtgctggg
     2401 attgatgctg ctcagcaccg gcagcatgct ggctggcaat tactttctta aaccactgat
     2461 caacgattac attctgccag gcgatttccc cggtctggcg aaggcgttgg tggtgctggg
     2521 agccatctat ctggtaggtg cgctggcatc ctatttgcaa agccgcctga tgatggtgac
     2581 cgccctgcgc acgaccaata ccctgcgccg cgatctgttc gccaggctgc aaaagttgcc
     2641 gttgaaatac tacgacaccc acgctcacgg cgatgtgatg agccgcttca ccaatgacat
     2701 cgacaacgtg caggtagccc tggaacaaag cctggtccag ctcctctcca gcgcgctgac
     2761 cttcaccggg gcggtggtgt taatgatcgt gctcagcccg atcctgttcc tggtcacctt
     2821 tctgatgctg ggattgatga ttttcctgat cgggaaaatc accaaaagga gcagccagtt
     2881 cttcaaagcc cagcaggcca acctgggcaa tgtcaacggc tatatcgaag agatggtgga
     2941 cgggctgaag gtggtcaaag tcttcacccg cgagcaggag gcgattgcag agttcaagct
     3001 gcgcaacgag gcctaccggc aatccgccac cagcgccaac tttaccgccg gagcggtgat
     3061 gccgatcatg ggcaacctga acaacatcgc ctacgccggc accgccatgt tcgggggaat
     3121 tctggcagtg gtatccggat tcgatatcgg ctcgctggca gctttcttgc agtatacgcg
     3181 caatgtgggc atgcccgtac agcagatcac caaccagctc aatgtgatcc tggcagccat
     3241 ggctggggca gaacgggtct tcgaggtgat ggacgaagct gaagaaaccg acagtgggac
     3301 ggtgaccctg gtggaggcaa cccggcaggc agatggcaag ctggtggctg gcaggaatgg
     3361 aaagcacccc gacctgcagg ctgatccagg ggttcctacg cggagctggg cgacccaaga
     3421 gagtggggcg acccaagaga tctgggcggc ccaaaagagc ggggcggccc aaaagagcgg
     3481 ggtgacccaa aagagctggg cgtggaaagt ccccaatggg aacaatgact ttcattatgt
     3541 cgagctgcag ggcgatgtgc gtttcctcga cgtaactttc ggttacgaag aaaataagac
     3601 cgtgctccac gatctctcac tgtacgccaa acccgggcag aaaatcgccc tggtcggctc
     3661 caccggagcg ggcaaaacca ccatcaccaa cctgatcaac cgcttttacg atatcgacga
     3721 ggggcagatc acctacgacg gcatcgatgt aaagcagatc cgcaaggacg acctgcgcaa
     3781 ctcgctgggg atggtgctgc aggacacgca cctgttctcg gggacaatca tggataacat
     3841 ccgttacggg cggctggatg ccacggacga agaatgcgtc cgggctgcca aacaggtcag
     3901 cgcccactcg ttcatcaagc gcctgccaga aggctaccag acgctgatca gcgccgatgg
     3961 cggcagcctc tcgcaggggc agcgccagct gctggcgatt gcccgcgccg ccgtcgccga
     4021 cccgccggtg atgatcctgg acgaagcgac cagctcgatc gacacccgca ccgagcgcct
     4081 gatcgagaag ggcatggatg ccctgatgaa ggaccgtact gtgttcgtta tcgcccaccg
     4141 cctgtcgaca gtacgcaatt cggatgccat cctggtgatc gaggatgggc ggatcatcga
     4201 gcgcggcgac cacgaggatt tactggagca gaagggcagg tactaccagc tgtacaccgg
     4261 gcagtaccag ctgagctgag aggagcaggg tgcggtcacc gcgaccgcac cctgctttta
     4321 tttcaccagc tcgtaaacct tctccattag ctcttccgcg ggtacctcat aatcgggggt
     4381 ccgggggagc tcgtttttcg agcgatcgat ccggaagaac tgcctatggg agactctgaa
     4441 taccagcccg gcatgtgggg tttgaatctg agcacatcgc cataggaagt gggcatattg
     4501 ccggaagtct caccgacgag ctcgcccagg cgattatcgg agatggcggt ggcaaaatcg
     4561 gtggctgagc tgaagtctgg accgcggtca ggacatagat cttcccggtg taggtcagct
     4621 ctggtatccg ctcattgggt tccccctcag acgaagtttg atacagataa ggcccgaagc
     4681 ggatatttgc cccgccagag attgcagcct gatccacatc cagatagcgc aggaattcgt
     4741 tgacgacctt tgaatttccg ctgccattgc ggcgcagatc gacggcgata ttctggatgc
     4801 cctggtcttt gacctcctgg aagaatgcgc acagggtatc cgtatagact tcattgaaga
     4861 tgcactgatt cagggtcagg atgcccagcc cggcggtgga atcaatctca taggagacga
     4921 aagactccgc ggtgagggat tgggactgcg gatatggtgt gcgcatggca gcgttcggtg
     4981 cactgtagtg cctgcctgcc ccggccctcg gaggtgatta tatgtgaatc tactggtttc
     5041 accatcctac ttcccgtctc cgccaaaagt accaggtgaa aatcccgctc aggatcgatg
     5101 tccatttcca aaaaagaggt tattctgcgg gtataatagc agcaaatcaa aaggttaaaa
     5161 agccgcaaac atcgggaact tttcacctcc cggcggcgtc tatgtaaagg gaaccaattt
     5221 agtaaaggag tatgcaaatc atgctgaaaa ccatttcatc tcgaaccatc tggatcgcac
     5281 tgagtcttgt gctggtcctc tcgatgcttg cagtggctgc tccgcagcca gtccaggcac
     5341 ccaccgacgc aacctgcgcc aagacggtga ccgtcaaaga aggtcagacc ctgcggcgaa
     5401 ttgccaaaca aaatgatgtc cgcctgtggc agctggcccg ggccaacgat atcgatgacc
     5461 cgtacgcccc attggaagtg ggcgacaagc tgtgcatccc cggtgaagta accttcagct
     5521 cgaaatccta ctggaccgcg actttcaaca ccagcggtga agtcaagatc atcgggcagg
     5581 gcttcaagaa gcaggccccc ttcttcctca aggtgcgtga gaacgccgat accaagcagt
     5641 tcaagctggc gaagctggtt accaccagca ccggtacctt caagaacact gaatacatgg
     5701 tccccaagga cctgcgggat aatccctacc tgttcgtctg cctgaaagat ggcatcacgg
     5761 atgccgttca atgcaagcag atctggcgct attagtccag ttgaattcaa aacgggcgct
     5821 gcggtgcagc gcccgttttt gattcccgaa ctttggagag atttagtcgc ctatggattc
     5881 tggaccaggg gcacgtacag gtagtgatcc ccaaccagca ggttgatgga ggcagtcccc
     5941 accccgccgt catcatccgc caccgtgacc gtgatcaggt aagttccggc gttaggatag
     6001 gcgtggctga ggctgaaagc ctgcccggtc agggccaggg gctggctgcc cgagccatcg
     6061 ccgtagttca cggtggcatt ccaggtatct gcacccggat cggtgaaggt gccctggcgg
     6121 gtgaacacag cccccaggtc gatataggta tccgtgccca ggttcaccac cggggcgacg
     6181 ttgaggaccg tgatcaggac agtgtcgctg tcgacagcgc cctgcccgtc gtccagggtg
     6241 agagtagcag tatagatgcc gttatcggcg taggtgtgga caggcgtcag gctgccagtg
     6301 agctcggggg taccgtcgcc aaagtcccag cttatattcc cgggcggcag gggaccgggc
     6361 aagtcggggt cctcaaagct gccggcgaac tgcaccgcag cgccttcgtt cacggtctgg
     6421 tctggaccgg catgggcaac gatggggata ttcgaactta ccgtgagggt caccgtggcg
     6481 acggcggaag tggccccatc atcgtcctgg acctggtagg tgaaggtcgc ggtaccagaa
     6541 aagttggtgg ggggtgtata ggtgaatcca ccgctggcct gcagcgccag ggttccgacg
     6601 gtcacatcac tgactatttg agctgtaagg gtatcggcag gtacatcgct gtcattttcc
     6661 agcacaccgg gcgctgagat cacggcagtc tggttgaaag gcaccgaata agcatcattc
     6721 acagccaccg ggctggcatt ggtgacactc accggcaaag tatccacatc ctggtagttg
     6781 ccgtcttcat cggtgagatg gatgcgcacc gtatggctgg caggtccatc gccaaagaca
     6841 tgcgtccggg tgccgccgag cgcgcccagg cagtcctgga gagggctgcc atcgccccaa
     6901 tccagctggc aggcgctgag ggtatcggcg ccgggatcga ctttcgggcc gatggtgagg
     6961 gtatagctgg caccttcaac cacactggca gccccggtca gggcgaccgt gggaaccaca
     7021 ttgttcactg tcaatacagc cacatcccca aggctggtcg ctccatccga cacactgatc
     7081 tcaacactga ataggccatt atcggagtaa gtgtgctgtt catcaaagaa gcggcctgtg
     7141 cccagcgcga ctaccgtact gggcgagcca tcaccccagc tgactgtcag ggtgaaattc
     7201 gccaggggat cggcaaccgt gaagctgccc agcagagtaa cggaagagcc ttcattggcc
     7261 ggattggggg tgagcgtata actgaggggc ggctcgctta aaccggtgcc ttcaccaaaa
     7321 aacactttcg ccaatccttc atccgcgccg gaaccgctct catatcccgg ggctccgacg
     7381 atgatatccc caaaattatc gttgttgata tcgcccgccc cggcaaccgc ccagccgaga
     7441 ttggcattgg gttggtttaa ctggtgcgtc cagtcgggga ttgcagctgg cccggcagcc
     7501 gcgccgaaaa ataccaacac agccccttct accgcttctg gatctgtata acgccaggcc
     7561 ccgaggatca gatcggcaaa actatccccg ttgatatccc cggcactcgc cacggagtta
     7621 cccaggtggg cgtctaaatt cccactgctg aaagaccaat ccggggtggt gctgggtcca
     7681 gaggccgcgc cgtaatacag aaaaactttc ccttcatcac ccagcgtacc atcaaaatga
     7741 tcggcgccga ccagcagatc gtcgatccca ccgccattga tatccccggc aactgcgact
     7801 gagctgccat actgggcgcc gatctggttg atctcattcg tccaatcggg aacggcagaa
     7861 ggcccgcctg ggccgccgaa atagatcgtt acctggcctt cgccagactg gccgttggac
     7921 cagcctggag cagccaggac aatatcgctg tggggatccc catttaaatt ccctgccgtc
     7981 agggcggtcc ccagcctggc attggcctgt ccaccgtaaa aggtgcgcgc cggagtggta
     8041 ctcagaccag atgcagagcc gtagtagagg tataccgcgc cttcatcgac aaactcgtca
     8101 tcataatagg gtgcgcctat caggaaatcg ccaaaaccat ccccgttgac atcgcctgct
     8161 tttgccaggg cgtaaccaaa acaagcattg gcctgggagc tgatcaaacg cggagttgcc
     8221 gtcagggata aaccggctgc gctgccttga aaaacatcca ccgcaccagc agatttatcg
     8281 ttggcggtac agattacaga gctgaccgct acatctgaat acacatcccc attgagcatg
     8341 ccggctgagg caaccgccca accataataa gcaaaggagg ttcccccgtt cttcgcccag
     8401 cccggctgcg ggcctaagcc tgcagcggac ccataaaaca ggtaagcggt gccttcagcc
     8461 tgcaggaaat tactgctctc atagaggtag gcaccgagca gaatatccgc atacccatcg
     8521 ccattgacat ccccggcgga ggcgagcgtg ctgcccagat ttgcaccagg ctgcccgcct
     8581 aaataactcc acacacccgg ggtagtttcc tgagctgaga cagaactggc tgcaaggagg
     8641 agtgccagaa ccagcagaca gctcagtccc tgccacagga ggttccggtt gatttttgac
     8701 agcaagcgtg gataattctt catatttagc acctctgttt ggctattatt tttcgatctg
     8761 agatggcgct gaagagactg ctgctctcag aatcgagaag ctggttaact gctttagatc
     8821 ccccctggat tgcctgtggt tttattatac tggtgtttaa gatggacata attgctaatt
     8881 tgataggtta acccacgggg agctgagcga agaatatctt caggcagctc ctggtggttt
     8941 gggaggcttc gtggcacccc ttcagcgcag ccggacactc ttataaatgg caggcgcacc
     9001 ggggcgagcc tgctcaggat cctgcgcgac agcagtttcc tgtgggcgca gccgctcagc
     9061 catggcagtg atgaacttcc agatcagcgg ggtgggctgg gcggctaaga aggccgcgtg
     9121 ggtatcgaga tccccatccc ggtagtgctt ttcgtcagac atagatccac ctccaaaaat
     9181 aacgagcgcg aaaagcggcg cctgatgacg ccgccaggta cccccaccgc gactcgaacg
     9241 cgggcttcta cctccggagg gtagcgctct atccactgag ctatgggggc atttcgtgcg
     9301 cgcccatttt accacgtttg gcaggcttct tcagcgaaat cttccacacc agggtgaatt
     9361 tccaggcgca tcgcccggac gcgatcctgc ctgcccggct ataatgagga ggtatgccta
     9421 accgccgcca atccatcccg cgaatcctta tcctgctggc aggtgtgtgc ctggcagcct
     9481 ggttgagcgc ctgcacggcg acccctccgc cgctatcccc cggcgactgg ctggtagaag
     9541 acctgctggc gctggaccca cccgaaaacc ggctcgccaa cgacccgctc acccgggccg
     9601 acctgattgc cgcctacgcc cggcccacgc tcgatgccat ccacctgcgg gtggacctgc
     9661 tcgattttcc ttccggacag gatgccgacc tgtacatcgc catcgattct ggctccaggg
     9721 gaacgaatat cctgcccctg gcaggcacca gcgagctcag ctgggaggtt ttgctggtga
     9781 tcccggcgat aggcgagctg caggtgctgg acagcgatct gaaccccatc cccgattctg
     9841 ccctgcgggt ggcgcgcagc cccggctcgc attcgatcgc cctcgccttc aatccagcca
     9901 gcctgcctgg agcggcaccg agtctgcgcc tggaattttt caccgccctg cccggcagcc
     9961 aggagatcgc cgaccgcctc ggaccgcaaa ctccccagac cgccatccca caggccatgc
    10021 cagccctgct ggctttttgg gattcgttcc cggctgccac ccctgcccag gcgctgcggc
    10081 gctgggatgg cgcccacacc gggccgctgg gcggccggca cgggctgttc aacctgctgc
    10141 gcaccgctcg cgcccgggaa attcccctgg tactgctcga tctgggcagc ccggaaaccc
    10201 tggcggcgct ggaatacgcc ggcgggatgg ggcttacggc ggagctggcg ctgagccggc
    10261 agcttattct gcccgccagc ctgccgggac tgctcgccca ggccggtgag gtactgccgc
    10321 tggtcgatgc ggagctgctg gcagcctggc tggaggccag ccgggagagc cagctggcgt
    10381 acggcctgcc cgccagcccc ttcgtgtctg ccccattgag cgccgcccaa ttctccggac
    10441 tggaagcaag cctgcagaca agcgcgggca ggacgatttt cacgttccgc gccggctcca
    10501 gcggcccatt cgggtacgca gcgcgccggg ggcagcagcg gctgctgatc ctgccgcagc
    10561 tgccggcagg gagcaccccc gaccgcctgg atttcgccct ggaggcgcgc cgcgagctgg
    10621 cccaggccgc ctgggactgg aacgcggccg aaactgatcc ggggcagttt ctgaccctgg
    10681 gcggcagcct gccggaatcg cccttcggca gcccggcata cgcccgcctg gcattccagt
    10741 acctgcagga gcacccctgg atccggctgt tgagtgagca tgatttgcga accctgccgg
    10801 ccagccggca cccgctggca gtaacctcca gcccaatccc tccaccaact tcggatctcc
    10861 aggcgcaagc ggtacaggcg gccccggaag cggctctgga actgccccag ccggctgtta
    10921 tcgcgctgcc ggtaagccgg tggggtgcgg atttcctgcg cgcaatctac acgcagccct
    10981 accctgcccc acccggcctg gcgagcctgc gcgccggcta cctgggaatg ctcagctcgc
    11041 tggcggaggc tgaggcctgg ctgaataacc cgaccccacg cctggaatgc agcggcgacc
    11101 cggaccagga aggcgcggcg gaatgtatcc tggccgacga aagcttctac gcccagatcg
    11161 agctggatga tgggcggctg gagctcggga tcgcccggcg ggatggcagc gccagagaag
    11221 ctggcggcgc acacctgctc attgccccgg gatacctgct ggtgagcggg ctcggcgacc
    11281 cggcaggctg gaacctggca gccggggagc gggcggaccc gcaggtgctg gccggcggct
    11341 tcggcggtgg agaggggccg tacaccgtcg aaatcctggc ggatggcttg cgcctgagca
    11401 gcccggatgg gacgacaatc aaggaatacc gcctggaaaa cggcgggctg gccttccaca
    11461 ttgccggcgc gccgggcagc ctctggcagc tgccgctggt gatcgagcct cagcagcgcc
    11521 agatgccagg ctggtggaaa gaatctgcct tcatgc
//
LOCUS       DATMXK010000556         3090 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_16983, whole genome shotgun sequence.
ACCESSION   DATMXK010000556 DATMXK010000000
VERSION     DATMXK010000556.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3090)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3090)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3090
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_16983"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1026)
                     /gene="tig"
                     /locus_tag="VN363_10165"
     CDS             complement(<1..1026)
                     /gene="tig"
                     /locus_tag="VN363_10165"
                     /EC_number="5.2.1.8"
                     /inference="COORDINATES: protein motif:HMM:TIGR00115.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005854 - nascent polypeptide-associated
                     complex [Evidence IEA];
                     GO_function: GO:0003755 - peptidyl-prolyl cis-trans
                     isomerase activity [Evidence IEA];
                     GO_function: GO:0051082 - unfolded protein binding
                     [Evidence IEA];
                     GO_process: GO:0051083 - 'de novo' cotranslational protein
                     folding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="trigger factor"
                     /protein_id="HWQ84923.1"
                     /translation="MKIETQPLDDHQVKLTLEVEPEAVEAAKHKAARHLSQRSKIPGF
                     RPGKAPYQVILRQYGEGAIFEDAIEHLVEDLYPQMIEEAEINPYGPGQLEKVVSMDPM
                     VLEFVVPLQAQVTLGDYTTVRVPFTPPEIGQDAVDTLMQELRERQAVLETVERPAQPE
                     DVVELKIRGERMNPEEGQNPVIIEEITAAILIKAEDAELDLKEWPYPGFSVNLAGKSA
                     GDEFTVEQSLPDDLEFAELRGVSARFSGVIETVKSRTLPELNDEFATMVSDKDNLDAL
                     TADIKNMLEFQGKQDYFDEYDNKVLDAAVELSEFKYPPQMIENEIDQFVESFKRRLKN
                     EELDFDLY"
     gene            complement(1130..1211)
                     /locus_tag="VN363_10170"
     tRNA            complement(1130..1211)
                     /locus_tag="VN363_10170"
                     /product="tRNA-Leu"
                     /inference="COORDINATES: profile:tRNAscan-SE:2.0.12"
                     /note="Derived by automated computational analysis using
                     gene prediction method: tRNAscan-SE."
                     /anticodon=(pos:complement(1175..1177),aa:Leu,seq:tag)
     gene            1182..1799
                     /locus_tag="VN363_10175"
     CDS             1182..1799
                     /locus_tag="VN363_10175"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84924.1"
                     /translation="MPVCQFQHFPDLHPDYTVSSLHRQQDVRYNPLAQPAGMLCVPGK
                     QGVAHVWGQPLQLTTTAREDLLSRVYNPESAGKDRSRLTRAVVISLRELMRQPEPNPT
                     TRDLAAFIALALNSIAETIDPSVAAWEKRGYWVKADRFRLDWAWTARLSDTLGKAVLA
                     ENWGEVAQAAVQIATKLQDVKVPVNHRMGTPWTGAWVKLRDSQIK"
     gene            complement(1814..3025)
                     /locus_tag="VN363_10180"
     CDS             complement(1814..3025)
                     /locus_tag="VN363_10180"
                     /inference="COORDINATES: protein motif:HMM:NF015983.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003824 - catalytic activity [Evidence
                     IEA];
                     GO_function: GO:0051536 - iron-sulfur cluster binding
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="radical SAM protein"
                     /protein_id="HWQ84925.1"
                     /translation="MDAFDRLKLLSDMMELEPAEEHTSPHLSTPGGKRTAPDNRGVHA
                     SRGLPDSRKAEALCIYPAVMPNGKRLPMLKTLLTSACERNCYYCPFRAGRDFRRATFK
                     PDEMAQTYMALHRAGIAHGLFLSSGVAAGGVHTQDLLIDTAEILRKKYAFGGYIHLKL
                     MPGAEYDQVLRAMQLTDRVSINLEAPTPEQLVRLAPSKYFYSELFQPLRWVEQIRQTQ
                     PEHLAWKGRWPSSTTQFVVGAAGESDLELLATTSHLHQQAGLGRAYFSSFHPIQDTPL
                     ENLPAENPARELRLYQASFLLRDYGFELEELPFKADGHLPLDRDPKLAWAHQHLSQQP
                     VEINRAGREELLRIPGIGPKSAKAILHARRVSSLCSLSDLKKIGVDPARATSFILLEG
                     KRPPVQLALDL"
BASE COUNT          666 a          832 c          866 g          726 t
ORIGIN      
        1 gtacaggtcg aaatccagct cttcattctt caaccggcgt ttgaaggatt cgacaaactg
       61 atcgatctca ttttcgatca tctggggtgg gtacttgaac tcagacagct caaccgcggc
      121 atccagtact ttgttgtcgt actcatcaaa gtaatcctgc ttgccctgga attccagcat
      181 atttttgata tcggctgtca gagcatccag gttatctttg tcgctgacca ttgtcgcaaa
      241 ctcatcgttt agctcgggaa gtgtgcgcga tttgacagtt tcgataaccc ctgaaaaacg
      301 ggcagaaacg ccgcgcagct ctgcaaattc cagatcgtct ggtaagcttt gctcaactgt
      361 aaattcatcc ccggccgatt tccctgccag attgacggaa aatcccggat aaggccattc
      421 cttgagatcc agctcggcat cttcagcttt gatcaagatt gccgctgtaa tttcttcgat
      481 gatgacagga ttctgccctt cttcaggatt cattcgctcg ccgcggatct tgagctcgac
      541 cacatcctcg ggctgggctg ggcgttcgac agtctcgagt acagcctggc gttcgcgcag
      601 ctcctgcatg agggtatcaa ccgcatcctg cccgatttcg ggtggagtaa atggcacccg
      661 gacggtggta tagtcgccca gggtgacctg agcctggagg ggaaccacaa attccaatac
      721 catcggatcc atgctgacaa ctttttccag ctggcctgga ccgtatggat tgatctcggc
      781 ttcctcgatc atctgaggat ataaatcttc caccagatgc tcgatggcat cttcgaagat
      841 cgccccttcg ccgtactggc gcaggataac ctggtaggga gctttacccg ggcgaaagcc
      901 agggattttg gatcgctgcg ataaatggcg ggcagcttta tgttttgctg cttccactgc
      961 ttcgggctcg acttcgaggg tcagcttgac ctggtggtca tcaaggggct gggtttcaat
     1021 tttcaaggct gggttcctga ctcaattagt atatttttct gctgtgaatt ttcccaaatt
     1081 ctgtgggatt ataacatgcg agtataagaa gagtgtaaga gtataggtgt ggggaagacg
     1141 ggaatcgaac ccgtacgcct tgcggcacat gatcctaagt catgcctgtc tgccagttcc
     1201 agcacttccc cgatttgcac ccggattata cggtatccag cctccaccgt caacaagatg
     1261 tgcgctataa tccgctcgcg cagccagccg gtatgctttg tgtgccgggc aagcaaggcg
     1321 tagcacatgt ttggggacag ccactgcagc ttacaacgac cgcccgggag gatctattga
     1381 gccgagttta caatcctgaa tcagccggca aagaccgcag ccgccttacc cgcgcggtgg
     1441 tgatctcgct gcgcgagttg atgcgccaac ctgagccgaa tccaactacc cgcgatctgg
     1501 cagctttcat tgccctggcg ctgaattcga ttgcggaaac catcgatcca tccgtggcag
     1561 cctgggagaa gcgcggttac tgggttaagg cagatcgctt tcgcctggat tgggcctgga
     1621 cggctcgctt gagcgatacc ctgggaaaag ctgtcctggc agaaaactgg ggggaggtgg
     1681 cgcaggctgc ggtgcagatt gcaacgaagc tgcaggatgt caaagtcccc gtaaatcacc
     1741 ggatgggtac gccctggaca ggcgcctggg ttaaattaag ggacagccag atcaaatagt
     1801 taaatgaccg gatctagaga tccaacgcca gctgcactgg tggacgtttt ccctccaaca
     1861 ggatgaacga cgtggcgcgc gcaggatcga ctccaatctt ctttaaatct gagaggctgc
     1921 acaggctgga aacacgcctg gcatgcagga ttgccttcgc tgactttggc ccaatgccag
     1981 gtatcctcag cagctcttcc cgcccggcgc ggttgatctc aactggctgc tggctgagat
     2041 gctggtgagc ccaggccaat tttgggtcgc ggtccagcgg aaggtgtcca tccgccttga
     2101 acggcagttc ttccagctca aaaccataat ctcgtaaaag aaaagaggcc tggtaaagcc
     2161 gcagctctct ggctggattt tcagcgggta gattttccag gggtgtatct tggattggat
     2221 gaaaactgga aaaatacgcc cgtcccaaac cggcctgctg gtgcaggtgg ctggtggtgg
     2281 ccagcagctc cagatcgctc tcaccggctg caccaaccac aaactgggtg gtggaggaag
     2341 gccagcggcc tttccaagcg aggtgctctg gctgcgtctg gcggatctgc tcaacccagc
     2401 gtaagggttg gaacagctct gagtagaaat atttcgaggg cgcaaggcgg accagctgct
     2461 ccggggtagg ggcctccagg ttgatagaca cccggtcggt gagctgcatg gcccgcagca
     2521 cctggtcgta ttcagctcct ggcatcaatt tcagatggat ataacccccg aaagcgtatt
     2581 tcttgcgcag gatctcggcg gtatcaatca acaaatcctg ggtgtggact ccgccggctg
     2641 caactccgga actcaagaat aatccgtggg caattccagc ccggtgcagc gccatgtagg
     2701 tctgagccat ttcgtctggc ttgaaggtcg cccggcggaa atcacgcccg gcgcgaaacg
     2761 ggcagtagta gcagttcctt tcgcaggccg aggtgagcag ggtcttgagc atgggcaggc
     2821 gctttccgtt tggcatcact gccggataga tacacaacgc ctcagcctta cggctgtcag
     2881 ggaggccacg gctggcatgt acgccccggt tgtcaggagc tgtgcgcttt ccgcctggag
     2941 tcgacagatg cggggaggta tgctcctctg ccggctcgag ctccatcatg tccgaaagca
     3001 gcttcaaacg gtcaaaagca tccatggctg accactcatt ctattttgcc ggtaaatatt
     3061 cagtcctggt tcatgcttgt tattatagat
//
LOCUS       DATMXK010000557         4808 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_166501, whole genome shotgun sequence.
ACCESSION   DATMXK010000557 DATMXK010000000
VERSION     DATMXK010000557.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4808)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4808)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4808
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166501"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..806)
                     /locus_tag="VN363_10185"
     CDS             complement(<1..806)
                     /locus_tag="VN363_10185"
                     /inference="COORDINATES: protein motif:HMM:NF017416.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="transposase"
                     /protein_id="HWQ84926.1"
                     /translation="MQAYPSLPPLPPETARAAKAVYSQNNFYLSTGSRLDILLADIQF
                     NSCTSPGGLYHLGKPLLALVTFFQYLEKLSDRQAAHATGARIDWKYALHLSMNYPGLN
                     LTDLCDFRQCLLREPIALQVFQQLLDRLSVDGPCPEFKDQTLDALDLLNEVCLRTRLD
                     KVATGMRHVLQALAIRSPEWLRSISLPYWYNRYGDAAGQPDLTQSMQQLRTLAEVTGA
                     DIVYLLDAIFSSDIPELAGLPEVQELHEIRSEQFDPICLDGVNLYPYCYF"
     gene            complement(1022..2803)
                     /locus_tag="VN363_10190"
     CDS             complement(1022..2803)
                     /locus_tag="VN363_10190"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84927.1"
                     /translation="MNPFDALERQIESIRQENSTSLIEVRNLLLEIEEFIDSADYQTL
                     PPDRRQWLQDARKELIATIDEQTGSDQPADPSEVDGQTPPPAIPQPLPGSSGTAEAGH
                     EHNPVAEQQIEVAEKLFYSGRYAEAIQIFDRVLQLEPNWERARQHRTEAENYLRTGYI
                     PVVALPADAASAFGKAQSASRVGRLADALALLEKAQANLRDLGIQRWQEGQEFAQKLQ
                     ENIDAENVYEEGLALFEDGEIDSAIEKVEAAVRPTGLPKYADKARQFRQVKESLRSIN
                     DTLTQAAVDPQAVSQAKSSLDGLLAEYGHNSAFDRLIDRFKTVVPRVIEPLTEQTRSL
                     KKQAERAQTLEESLYLARQAKQNLDQIRNLTGVDESLDHLQSDIEKLQRDILRFENDL
                     QSAQRAYENHPTWPSEAARLGAEVMTRYPIDPGVSTLKRNLRSYSLKLTAVRAGIALV
                     GIILLVIFANLGLNRYRAYLVSLTPTATPTITLTPTLTLTPTSTGTLTPTITPTPLPS
                     STPLPISSIAQRDIWARSGCYEGFSAVGRIRSSGVVRFLPSERRFDDFNRECALVEYQ
                     RETGDVIGWVLLADLGAEPPPLVTPTP"
     gene            complement(2870..>4808)
                     /locus_tag="VN363_10195"
     CDS             complement(2870..>4808)
                     /locus_tag="VN363_10195"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84928.1"
                     /translation="LREIAGLAQLPDAPMPAAFQEKLASALASGVVDEWPALLWDIFS
                     LIGDGPVPEWWEALSRRVRRIHLNLDEETLPPIVTVNRLYYTLQASIADTQGKLPRID
                     SEARLQAGSRLEQLDNLLKIFDNEVVKKWKEREPAPPNSGISYQELAALIEEIESILP
                     GSAQKIQLALSQPQAQAEIALNAWEHRDFETARKALRRILIWDPDRWRLLVADRTIAQ
                     AGAWLAAVRSGAPRDVPFYDFLSATELSGRRLRNRVGSASWLDGILNALRRLRKGSKS
                     ADLVIDLPEIAGDIPWLNEFRSRETLTLSRSRPLTLERDLNGTSAPVTLQGVQEAKFG
                     AGQELALGEPLDTWVPEARGSSARVFSGTLRDRAGKDQSYAIKIMRPDRAEYSLPLFR
                     EEAHILSLLRGVPGVTPLVECGFLRLEEGVELPVEDRHLSAAYLSGQAVRFGAEQVQN
                     YLSSMDRYLSQSWIPYLALEQRDHNLNLLKFCDVGFTHGWFLPLRTSLLLSIQICDIL
                     QTAHDRNIVYRDHKILHYYWDPRSHGVISIDWNIAKRHAEGLTEPERQFDLVQFGARA
                     LHHILTGRPAPGALPLGPNRPDEIEHASTQYTVNWTYDDERLPNQIKEILEQVLNQGY
                     AQVKTLRADLAGLYEQLPESE"
BASE COUNT         1066 a         1234 c         1476 g         1032 t
ORIGIN      
        1 caaaagtaac aataaggata aagatttacg ccatccaggc aaatgggatc gaattgttca
       61 ctccggattt cgtgcaattc ctgaacttct ggcaagccgg ccagctccgg gatgtcggag
      121 ctgaagatgg catccagaag ataaacgata tccgctccgg taacttctgc cagggtgcgg
      181 agctgctgca tgctctgggt cagatctggc tggccagccg catccccata gcggttgtac
      241 caatacggca ggctgatact gcgcagccat tcgggggagc gaatagccaa tgcctgcaaa
      301 acatggcgca tgcctgttgc caccttatcc aggcgggtac gaagacatac ctcattcagg
      361 aggtccaggg catcgagcgt ctgatccttg aattccggac agggaccatc gacgctaaga
      421 cgatccagga gctgctggaa tacctgcagg gcgattggct cgcgcagcag gcactggcgg
      481 aagtcgcata agtcagtaag gttcaagcct ggataattca tggatagatg caaggcatac
      541 ttccagtcaa tacgagcccc ggtcgcatgc gctgcctggc gatcggataa cttttccagg
      601 tattggaaaa acgtcaccag ggcaagcagc ggtttcccca agtgatatag tcctcctggc
      661 gaggtacagc tgttgaattg gatatccgct aaaagtatat ccagccggct gccggtggac
      721 aaatagaaat tgttctggct gtagacggcc tttgcggcac gcgctgtttc gggcggcagt
      781 ggtgggaggg aaggataagc ttgcataacg tactgttccc aatatctcag atgaaccggt
      841 caggtttgga atgcatgcaa tcacactata cataaaaact ttctgaatca taactggaga
      901 aatcccctgt tttttcaggc taaacatgta tcaactacgt agtggaagga tacggggtga
      961 aaaaaatcag cgcgtagtaa ctacgcgctg attgcggatc caacgatcct tcagaggctg
     1021 gctaaggtgt gggtgtgacc aggggcggag gttccgctcc caggtcagcc agcaagaccc
     1081 agccaatcac atccccagtt tcgcgctggt attccacgag ggcacattca cgattgaaat
     1141 catcaaaccg ccgctcggag gggaggaaac gcaccacccc gctggagcgg atcctgccta
     1201 ccgcactgaa gccttcatag caaccactgc gggcccagat atcgcgctgg gcgatactcg
     1261 agatgggcag aggggtactg ctgggcagcg gggtgggagt gatggtaggc gtcagagtcc
     1321 cggtggaagt gggagtcagc gtcagggttg gtgtgagcgt aatcgtgggg gtggcggtgg
     1381 gggtgaggga gaccaggtaa gcccgatagc gattcagacc caggttggcg aagatcacca
     1441 ggagaatgat ccccaccagg gcaatacccg cccgaacagc ggtcaatttc aaggagtagc
     1501 tgcgcaaatt acgcttcaaa gtgctgacgc caggatctat gggatagcga gtcatcactt
     1561 cagcacccag gcgggcagct tcggagggcc aggtggggtg attttcgtag gcgcgctggg
     1621 cggattggag atcattctca aagcgcagga tatcgcgctg cagcttttca atatcgctct
     1681 gcaggtgatc caggctttca tcgacaccgg tcaggttgcg aatctgatcg aggttctgtt
     1741 tcgcctggcg agcgagatag agcgactctt ctaaggtctg ggcgcgttct gcctgtttct
     1801 ttaatgaacg agtctgctcg gtgaggggct cgatcacccg tggaaccacg gttttaaacc
     1861 gatcgatcag acggtcaaag gcggagttgt gaccatattc agcaagcagg ccatccaggc
     1921 tggatttagc ctgtgaaact gcctgagggt caacggctgc ctgggtaagg gtgtcgttga
     1981 tcgagcgcag ggattctttt acctggcgaa actggcgagc tttgtcggcg tatttgggaa
     2041 gcccggtggg gcggacggca gcttcgacct tttcgatggc gctgtcgatt tccccgtcct
     2101 caaagagagc cagaccttct tcataaacat tttcggcatc aatattttcc tggagtttct
     2161 gagcaaactc ctggccttcc tgccagcgtt gaatgcctaa atcgcgcagg ttcgcctggg
     2221 ctttttccag gagcgccagg gcatcggcaa gacgacccac gcgagatgca gattgggctt
     2281 ttccaaacgc tgaggcagca tcggcgggaa gggcgacgac cgggatatag ccggtgcgca
     2341 agtagttttc cgcctcggtg cggtgctggc gggcacgctc ccagttaggc tcgagctgga
     2401 ggacccggtc aaatatctgg atggcttcgg cataacggcc gctgtaaaat aatttttcgg
     2461 cgacctcgat ctgctgttcc gctacagggt tgtgctcgtg ccctgcctcg gcggtgccac
     2521 tactaccggg caggggttgg gggatcgccg gcggcggggt ctggccatcc acctcagacg
     2581 gatcggccgg ctggtcagaa ccggtttgct catcgatcgt ggcgatcaat tctttacggg
     2641 catcttgcag ccactgacgt ctgtccgggg ggagggtttg ataatcagct gagtcgataa
     2701 actcttcgat ctctaagagc agattacgaa cctcaatcag gctggttgaa ttttcctggc
     2761 ggatcgattc aatttggcgt tcgagggcat cgaatgggtt catggttgca tccatctggg
     2821 tattatcacc taagcggata aatgaccgtt tagaaaattg ctttgatttt tattctgatt
     2881 caggtaactg ctcgtacaga ccggccaggt ccgcacgcag ggttttgacc tgagcatagc
     2941 cctggttcag cacttgttcg agaatttctt tgatctggtt gggcagcctt tcatcgtcat
     3001 atgtccaatt gactgtgtat tgagttgagg cgtgttcgat ctcgtccggg cggtttggac
     3061 ccagaggtaa ggcgcctggc gcaggccgtc cggtaaggat gtgatgcagg gcgcgggcgc
     3121 caaactggac cagatcgaac tgcctttcag gctcggtcag tccttcggcg tgacgtttgg
     3181 caatattcca gtcgattgaa atgaccccgt gcgagcgagg atcccaatag taatgcagga
     3241 ttttatgatc gcgatagacg atattgcggt catgcgcagt ctggaggata tcgcaaattt
     3301 gaatggacag cagcaagctg gtgcggaggg gtaaaaacca gccgtgggta aagcctacat
     3361 cacagaattt cagcaaatta agattgtggt cgcgctgctc cagggccagg tagggaatcc
     3421 agctctgact cagatagcgg tccatggaag aaagataatt ttgaacctgc tccgcgccga
     3481 aacgcactgc ttgaccgctt agataggcgg cggataaatg gcgatcttca actggcagct
     3541 cgacaccttc ttccaggcgc aaaaagccac actccaccaa cggggtgacg ccggggacac
     3601 cgcggagaag agaaaggata tgggcttcct cgcgaaacaa gggcagcgag tattccgccc
     3661 ggtctgggcg catgattttg atggcatagg actggtcctt accggcgcga tcgcgcaaag
     3721 tgccggagaa gacgcgcgcc gacgagccgc gggcttctgg aacccaggta tccaggggct
     3781 cacccagggc caattcctgg ccagcgccga atttagcttc ctggactccc tgcagggtga
     3841 caggggcgga ggtgccattc agatcgcgtt caagcgtcag cgggcggctc cgggacaagg
     3901 tgagagtctc gcgggacctg aattcattga gccagggaat atcgccagca atttcgggga
     3961 ggtcgatgac cagatcggct gatttggagc ccttacgcag ccggcgcagc gcgttcagga
     4021 tgccatccaa ccagctggct gagccgacgc gattgcgcag cctgcgaccg gatagttcgg
     4081 tggcagacag aaaatcataa aagggcacat ccctgggagc accactgcgg acagctgcca
     4141 gccaggcgcc ggcctgggcg atggtccgat cggcaaccag caagcgccag cgatcgggat
     4201 cccagatcag gatgcggcgg agggctttgc gagcggtctc gaaatcgcgg tgctcccagg
     4261 cattcaaagc aatctcagcc tgagcctggg gctggctgag ggctaactgg atcttctggg
     4321 cagagccggg caagatcgat tcgatctcct cgatcagcgc ggcgagctcc tgatagctaa
     4381 tccccgaatt ggggggggca ggttcgcgct ctttccattt tttcaccact tcattatcaa
     4441 agatcttgag aagattatcc agctgctcca gccgggaacc agcctggaga cgggcctcag
     4501 agtcgatcct gggcagcttg ccctgggtgt ctgcgatgga agcctgtaag gtgtagtata
     4561 gacggttgac cgtgacgatg ggggggaggg tttcttcgtc cagattcaga tgaatgcggc
     4621 gaacgcggcg gctgagggct tcccaccact cggggacagg accgtcgcca attaaggaga
     4681 atatatccca gagcaaggct ggccattcat ccacgacacc ggaggccagg gcgctggcca
     4741 gtttttcctg gaatgcagcg ggcatgggtg catctggcag ctgtgccagc ccggcaattt
     4801 ctctcagc
//
LOCUS       DATMXK010000558         2178 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_29268, whole genome shotgun sequence.
ACCESSION   DATMXK010000558 DATMXK010000000
VERSION     DATMXK010000558.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2178)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2178)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2178
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29268"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(262..2037)
                     /locus_tag="VN363_10200"
     CDS             complement(262..2037)
                     /locus_tag="VN363_10200"
                     /inference="COORDINATES: protein motif:HMM:NF015557.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SLC13 family permease"
                     /protein_id="HWQ84929.1"
                     /translation="MTFEIALLLGIIILALVLFSVERFPTDIVAIGLLILLVVTGLLP
                     VERAFAGFGSDAVIVIFCLLVLTAAMIRTGVVEQIGRVIIRRVGNNPNRVLAASLIAP
                     AMLGAFISNTASAAFFLPVLIGVGRQAKISISRLLMPMAFATILTSSVTLISTSTNIV
                     VSSLMVQNGQEPLGMFELAPVGIPIAIIGIIYMYFIGIRLVPDRVPTQNMLGELGTKK
                     FLSEIRVEPDSSLVGKTLAQSGLGRDLDLTVLRLVRGDTGVLIPQADTVLEADDVLVV
                     EGWRDEILKVHTTSGIKFKADVEVAASVLQDKAIRLAEVILLLRSPLVGRSLARLKLR
                     EQYGIQVLAISRHGETIDRKLSRIVLQLGDILLIQGHINSIAALEGDKAFRILTDVDK
                     PYYNKRQGWITVGAFVGSLLLAAFGLVSLPVAFLIGCLIVFVTRCITPEEAYREIEWK
                     AIILIGSMLAMGAALDHTGAAKYLAAQIVTLVGDQSPVYLLGGFFILCVLLTQPMSNQ
                     AAAVVVVPIAIHTAAQLGLNPRTFTVMIAIASSCSFITPLEPANLMVYGPGRYKFFDF
                     VKVGSLLTVIIFLISIFLVPIIWPL"
BASE COUNT          546 a          637 c          551 g          444 t
ORIGIN      
        1 gcagccagac ctttaccttc gaaatccccg cttccctgca gggctcacgc cagattgcca
       61 tccgcatcga aagcaccagc ggctccggtt tcttcgccta caactggttc tacaacacca
      121 ctgcgtaatc cagtccgggg agtttgccag tcagcaaagc agaattattc tcgtaccatt
      181 acccacaaag ggcgaagcag gtttatctgc gccatccctg gcagataaga cttgcttcgc
      241 ccatattatt agacgtcctg gttacaatgg ccagatgatt ggcaccagga agatcgagat
      301 caaaaaaatg atcacggtca gcagtgaacc cactttaaca aaatcgaaaa acttatacct
      361 gcctggccca tacaccatca ggttggctgg ctccagaggt gtgatgaagg agcagctgga
      421 ggcaatcgca atcatcactg tgaaagtgcg tggattcaat cccagctggg cggcggtatg
      481 gatcgcgatc ggcacgacaa ccacggctgc tgcttgattg gacatcggct gagtgagcag
      541 cacgcacagg ataaaaaatc ctcccagcaa atacacgggg gattggtccc cgaccagagt
      601 aacgatctga gccgccagat attttgcagc gccggtatga tccagggctg cccccatcgc
      661 cagcatactg ccgattaata tgatggcttt ccactcaatt tcacggtaag cctcttcagg
      721 ggtgatgcag cgggtaacga aaacgatcag gcagccgatc aaaaacgcca ccggcagtga
      781 caccaatcca aacgcagcca gcagcaggga tccgacaaac gctcccactg tgatccagcc
      841 ctggcgtttg ttgtagtagg gtttatcaac atccgtgagg atccggaacg ccttatcgcc
      901 ttcaagcgcg gcaatactgt taatatgacc ctggatcagc aggatatcac ccagctgtaa
      961 gactatccgg ctgagcttgc ggtcgatcgt ctcaccatgc cggctgattg ccaggacctg
     1021 gatgccatat tgctcacgca gcttcaggcg ggccagggat cttcccacca gaggcgagcg
     1081 caaaagcagg atcacctcag ccaggcgaat cgctttatcc tgcaaaacag atgcggcgac
     1141 ctcgacatcg gctttgaatt tgatcccgct tgtcgtatgg actttcaaga tttcatctcg
     1201 ccagccctcc accaccagga catcgtccgc ttcgagcacg gtatcggcct gcggtatcag
     1261 gaccccggta tcccctcgga ccagccgcag cacagtcagg tcaaggtccc tgcccaggcc
     1321 agattgagcc agggttttac ccaccagcga gctgtccggt tccacccgga tttcactgag
     1381 gaatttcttc gtccccagct cgccgagcat attttgtgtt ggaacgcgat ccggtaccag
     1441 acggatgccg ataaagtaca tgtagataat tccgatgatg gcgatcggaa tccccaccgg
     1501 agccagctcg aacatcccca ggggctcctg accgttttga accatcaggc tgctgaccac
     1561 aatatttgta gaagtggaaa tcaaggtgac cgagctggtc aggatcgttg caaatgccat
     1621 gggcatgagc aggcgtgaga tgctgatctt cgcctgccgc ccgacaccga tcagtaccgg
     1681 cagaaaaaat gctgccgagg ctgtgttact gatgaaagca cccaacatcg caggggctat
     1741 caaagaggct gccagaactc ggtttgggtt attccccact cgccggatga tcactcgccc
     1801 aatctgctcc accactcccg tccggatcat ggccgccgtc aggaccagca ggcagaaaat
     1861 cacgatcacg gcatcactgc caaatccggc aaatgcccgt tcaaccggca gcagcccggt
     1921 taccaccagc aggatcaaca gcccgatagc aacgatatcg gttggaaacc gctcaaccga
     1981 gaacaggacc agagccagga tgatgatgcc taacagcaag gcgatttcga atgtcatcgg
     2041 cttccttgat gttaataggg gaataaattg aatgcattat acttgacttc cctatatccc
     2101 tatggtaaac taccgtccgc aagcaaggtc ccgtggtgta gtggcctaac atgcggccct
     2161 gtcaaggccg agatcgcg
//
LOCUS       DATMXK010000559         2071 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_120499, whole genome shotgun sequence.
ACCESSION   DATMXK010000559 DATMXK010000000
VERSION     DATMXK010000559.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2071)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2071)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2071
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120499"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..836
                     /gene="plsX"
                     /locus_tag="VN363_10205"
     CDS             <1..836
                     /gene="plsX"
                     /locus_tag="VN363_10205"
                     /EC_number="2.3.1.274"
                     /inference="COORDINATES: protein motif:HMM:TIGR00182.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043811 - phosphate:acyl-[acyl carrier
                     protein] acyltransferase activity [Evidence IEA];
                     GO_process: GO:0008610 - lipid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphate acyltransferase PlsX"
                     /protein_id="HWQ84930.1"
                     /translation="LKAKRRGSQTSMAVGIDLVINGEADAFVTAGNTGGALATAYYRM
                     GVIPGVERPGLTAYFPVKDGVCAVVDIGANPECKPVHLLQFAIMASVYASKLRGIPNP
                     RVGLISNGEEAGKGNELVHGAYPLLEASGLNFIGNIEGKELFGGKADVAVTDGFTGNV
                     LLKSSEAVAKLLIDTLKQELKSSPLSMLGAALAKPAFRKIKVLLDPGAIGAAPLLGVN
                     GLVFIGHGRSDAYAMLSAIQTARSAVEANLLSALQSAIAERLALSEAPASLPTMEMTD
                     L"
     gene            833..>2071
                     /gene="fabF"
                     /locus_tag="VN363_10210"
     CDS             833..>2071
                     /gene="fabF"
                     /locus_tag="VN363_10210"
                     /EC_number="2.3.1.179"
                     /inference="COORDINATES: protein motif:HMM:NF005589.0"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004315 - 3-oxoacyl-[acyl-carrier-protein]
                     synthase activity [Evidence IEA];
                     GO_process: GO:0006633 - fatty acid biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="beta-ketoacyl-ACP synthase II"
                     /protein_id="HWQ84931.1"
                     /translation="MSQRAVITGMGAITCLGLNVAELWEGLVNGRSGIRRITQFDASN
                     FPCQIAGEIEGFTPEDYLDRKEARRVPRSSQIALGAATQAVQAACLTSDGCFPDPERT
                     GVAFGTAIGGIDHIDIGLQVLRTYGFEKVNPFTIPTGIPNLPAFMVARHYQCLGPNIT
                     ITTACATGTQVIGEAAEWIRRGAVDVVITGGTEALIRDFAIGGFAAMRALPTHFNDRP
                     EAASRPFDLNREGFVFSEGAGAMVIESLEHAQRRGAHIFAEVAGHASSTDAFHMAAPE
                     PNGHGPGRAMRWALQDAGLTPEAVDYINAHGSSTPLNDTTETRAIKDVFGEHAYRLAI
                     SSTKSMLGHAMGASGMLEAIACVKAINENCLPPTINYETPDSECDLDYVPNQARSAVV
                     NVTLSNSFGLGGQNACVVIKR"
BASE COUNT          418 a          617 c          628 g          408 t
ORIGIN      
        1 ccttaaaagc caagcggcgc ggctcgcaaa cttcgatggc agttgggatt gatctggtga
       61 tcaacggtga agcagatgcc ttcgtcacag ccgggaatac gggcggcgcc ctggcaaccg
      121 cctattaccg gatgggagtg atccccgggg tggaacgccc cggcctgact gcctattttc
      181 cggtcaaaga cggcgtgtgc gcggtggtag atatcggcgc caacccggaa tgcaagcctg
      241 tccacctgct gcagtttgcc atcatggcca gcgtgtatgc cagtaagctg cgcggcatcc
      301 ccaaccctcg cgtcggcctg atctccaatg gggaggaggc cggtaaaggg aatgagctgg
      361 tacacggcgc ctacccactc ctggaagcca gcggactgaa cttcattggc aatatcgaag
      421 gcaaggagct atttggcggc aaggctgatg tagcggtaac cgatggattt acgggcaatg
      481 ttttgctcaa atcaagcgag gccgttgcca agctgctcat tgataccctg aagcaggagc
      541 tgaaatccag cccgctgtcc atgttgggcg cggcgctggc gaaacctgcc ttccgcaaaa
      601 tcaaggtact gctcgatccc ggggcgatcg gggcggcgcc gctgctgggc gtcaacggcc
      661 tggtatttat cgggcacggg cgctcggatg cctacgccat gctctcagca atccagactg
      721 cccgcagcgc agtggaggcg aacctcctgt cggctctcca gagcgccatc gctgaacggt
      781 tagccctcag cgaggcgcct gcctccctgc caaccatgga gatgactgat ttatgagtca
      841 acgcgcagtc atcaccggca tgggtgcgat cacctgcctg ggtcttaatg tagcagaact
      901 ttgggaagga ttggtcaatg gtcgctcggg gattcgccgg atcacccagt tcgacgcttc
      961 caatttcccg tgccagatcg caggcgaaat cgagggtttc accccggaag attatctcga
     1021 ccgcaaagag gcgcggcgtg tcccacgctc atcccagatt gcacttggcg cagccaccca
     1081 ggctgtccag gctgcctgcc tgacctcaga tggctgtttt cctgacccgg aacggaccgg
     1141 tgtggctttc ggaacagcaa tcggtgggat agaccatatc gacataggcc tgcaggtcct
     1201 gcgcacttat ggttttgaaa aggtcaaccc tttcacgatc ccgaccggga tcccgaacct
     1261 gccggctttc atggtcgccc gccattacca gtgcctggga cccaatatca cgatcacgac
     1321 ggcctgcgca accggcaccc aggtcattgg agaggctgcc gaatggatcc ggcgcggggc
     1381 ggtagatgtt gtgattaccg gagggaccga agcactgatc cgagatttcg ccatcggcgg
     1441 gtttgcggcc atgcgcgcct tgcccacaca ttttaacgac cgcccggagg ctgcctcccg
     1501 gccattcgac ctgaaccgag agggttttgt gttctcggaa ggggcaggag cgatggtgat
     1561 cgagagcctg gaacatgcgc agcggcgtgg ggcgcatatt ttcgccgaag ttgccgggca
     1621 cgcctcctcc acagatgctt tccacatggc agccccggag ccgaatggtc atgggccggg
     1681 acgcgccatg cgttgggcac tgcaagacgc cggtttgacc cccgaagctg tggattacat
     1741 caatgcccac ggctcctcca caccgctcaa cgacaccacc gagacgaggg ccatcaagga
     1801 tgtttttggc gagcatgcct accggttggc gatcagctcc accaagtcga tgctgggcca
     1861 tgccatgggc gcttcgggga tgctggaagc gatcgcctgt gtgaaggcga tcaatgaaaa
     1921 ctgcctgccg ccgacgatca attatgaaac ccccgactcc gaatgcgatc tggattacgt
     1981 ccccaatcag gcgcggtcgg cggtggtgaa tgtgacttta tccaattctt ttggcctggg
     2041 cgggcaaaat gcctgcgtcg taatcaaacg c
//
LOCUS       DATMXK010000560         2682 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_29592, whole genome shotgun sequence.
ACCESSION   DATMXK010000560 DATMXK010000000
VERSION     DATMXK010000560.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2682)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2682)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2682
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_29592"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            103..585
                     /locus_tag="VN363_10215"
     CDS             103..585
                     /locus_tag="VN363_10215"
                     /inference="COORDINATES: protein motif:HMM:NF014305.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta fold hydrolase"
                     /protein_id="HWQ84932.1"
                     /translation="MSQNQLLYIHGLQGTSQGIKAQLLRRDFPDILVPDFQGDLDTRM
                     AALEAILGNQTGWTLIGSSFGGLMAAMFATRRPTQVKRLILLAPALIWPDFANQPPPA
                     IDIPTLIYHGRQDQIVPLDLVQELAQQVFTNLEFHAVDDDHGLYETVHSLDWKGILAG
                     "
     gene            590..787
                     /locus_tag="VN363_10220"
     CDS             590..787
                     /locus_tag="VN363_10220"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84933.1"
                     /translation="MVNAQAVTLVDADEVASEMGEYLQAAPQIAKYFQVRLDAQGQPV
                     KDDILAAARSRVSVRILLNPN"
     gene            complement(828..>2682)
                     /locus_tag="VN363_10225"
     CDS             complement(828..>2682)
                     /locus_tag="VN363_10225"
                     /inference="COORDINATES: protein motif:HMM:NF012772.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="EAL domain-containing protein"
                     /protein_id="HWQ84934.1"
                     /translation="PGSFTDEDEARLTDLADYASVAIENASLYEQAQQEILERKRIEQ
                     ALRESEERYALAVQGANDGLWDWDLRSNQIYYSPRWKSILGLAETEVGHHPDEWYNRV
                     HLEDIERLKLDISAHMAGLTPHFENEHRILHKDGEYIWVQSRALAVREEDGVAYRMAG
                     SLTDITDRKNAEQRLLHDAFHDTLTGLPNRALFLDRLRQAVERSKRRTDFTYAVLFLD
                     LDRFKDVNDTLGHMLGDQLLVIIGKMLESGLRSTDTVARLGGDEFVILMEDITNIQGA
                     MRVANWVHEMFKTPIHVLDHQVFISTSIGIVSGGLNYARPEDILRDADIAMYSAKAHG
                     RARSEVFEPEMRVRISRRLLIESELRQAIEHEEFVIHYQPIISLEDQRITGFEALVRW
                     NHPIRGLLIPADFLYVAEESGLIVPIDRWVLRQACFQMKTWQDQYPIQIPLTISVNLS
                     GRHVTRPDLVDYIQNVLKETRLAPTCLKLEITENILMETNLLTSETFHRLQALGVQLQ
                     IDDFGIGYSSLSYLSQFPVNALKIDQSFIKKMAVDNSQLEIVQAIVMLAQRLDVVVVA
                     EGVETAAQLHQLQSLGCEYGQGFYVSKPLESAGIEAILAGSAGKGLLMDSL"
BASE COUNT          633 a          743 c          689 g          617 t
ORIGIN      
        1 cgcccgagcc aactcgattt ttcagctgca gccggttcgt ttgataaaat ggggtggtac
       61 ctgtttggta ttatccgggc agatggatag aagagggcga ggatgagcca gaaccagtta
      121 ctgtatatac acgggcttca aggcaccagc cagggcatca aggcgcagct gctgcgccgc
      181 gacttccccg atatcctcgt ccccgatttt cagggcgacc tggatacccg catggctgcc
      241 ctggaagcga tccttgggaa tcagactggc tggacgttaa tcggctccag cttcggggga
      301 ctgatggcag caatgtttgc cacccgccgc cccacccagg tgaagcgcct tatcctgctg
      361 gctcctgctt tgatctggcc tgattttgcc aaccagcctc caccggcaat cgacataccc
      421 accctgatct atcacgggcg acaggatcag atcgtgccat tggacttagt ccaggagctg
      481 gctcaacagg tttttacaaa cctggaattt catgccgtag atgacgacca cggtctgtat
      541 gaaaccgtcc acagcctgga ttggaaaggg attctggctg gctgacaaag tggtcaatgc
      601 ccaggcagtc accctcgttg atgcggatga ggtagccagt gaaatgggag aatacctgca
      661 ggctgcacct caaatcgcta aatactttca ggtacgcctg gatgctcagg gccagccggt
      721 gaaagatgac attcttgcag ctgccaggag ccgggtcagt gtgcgtattc tgctaaatcc
      781 gaattaaacg cggcaattca aaactattaa atagctggcc gggttggtta cagactgtcc
      841 atcaaaagcc cttttcctgc cgaacctgcc aggatagctt caatcccggc cgattccaac
      901 ggtttggata catagaaccc ctgcccatat tcacaaccca atgactgaag ctgatgtaat
      961 tgcgcggccg tttcaacgcc ctcggctacg acaactacgt ccaggcgttg ggcgagcatt
     1021 acgatagcct ggacaatttc tagctgcgaa ttatcgacag ccatcttttt aataaaggac
     1081 tggtcgatct tgagggcgtt gactggaaac tgcgagaggt agctcagaga tgaataacca
     1141 atcccaaaat catcgatctg gagctggacc cccagcgcct gcagtcgatg aaaagtttca
     1201 ctggtaagca ggttggtctc catcagaatg ttctcagtaa tctccagttt caggcaggtg
     1261 ggagccagcc gggtttcctt gagcacattt tgaatataat ccaccaggtc tggtctggta
     1321 acatgtctgc ccgacagatt gacactgatt gtcagcggaa tctggatcgg atactggtcc
     1381 tgccaggtct tcatctggaa gcaggcctgc cgcagtaccc aacgatcaat cgggacgatc
     1441 agccccgatt cctcagccac gtatagaaaa tcagccggaa tgaggagccc acgaatggga
     1501 tgattccagc ggactaatgc ctcaaacccg gtgatgcgct gatcttccag ggatatgatt
     1561 ggctgataat ggatcacaaa ttcctcatgc tcaattgcct ggcgcagctc ggactcgatc
     1621 agcaagcgtc gcgagatccg aacccgcatc tctggttcaa agacctctga gcgcgcccgt
     1681 ccatgggctt tggcactgta catggcgata tcggcgtcac gcaatatatc ttcggggcgg
     1741 gcataattca atccacccga aacaatcccg atgctggtgc tgatgaatac ttggtgatcc
     1801 agcacatgga tcggggtttt aaacatttca tggacccaat tcgccacgcg cattgcgccc
     1861 tgaatattcg tgatatcctc catcaagatc acgaattcat cgccgcccag ccgcgccacc
     1921 gtatccgtcg agcgcagacc gctttccagc attttaccga tgatcaccag gagctggtcg
     1981 cccagcatgt gccccagggt gtcgttgaca tctttgaaac gatccaggtc cagaaataaa
     2041 accgcataag tgaaatctgt gcgccgcttg gagcgctcga ccgcctggcg cagtctgtcc
     2101 aggaataaag ctcgattagg cagcccggtc aatgtatcgt ggaatgcatc atgtaagagg
     2161 cgctgctcgg cgtttttacg atcggtgata tctgtgagtg agccggccat ccgataggct
     2221 acgccatctt cctcgcgcac agccagggcg cggctctgca cccagatgta ttcaccatcc
     2281 ttatgcagga tgcggtgctc attttcaaaa tgcggggtca atccagccat atgagcagag
     2341 atatccagct taaggcgttc gatatcttcc agatgcaccc ggttgtacca ttcatccgga
     2401 tgatggccga cctcagtctc tgcaagaccc agaatagatt tccagcgtgg ggaataatag
     2461 atctggttgc tgcgtaaatc ccaatcccac aaaccatcat tggcgccctg aacagccagg
     2521 gcatagcgtt cttcactttc ccgcagggct tgctcaattc gcttgcgctc gaggatctcc
     2581 tgttgagcct gctcatataa ggaggcattc tcaatggcta ccgaagcata atcggccaga
     2641 tctgtcagcc gcgcctcatc ttcatccgta aatgagcctg gg
//
LOCUS       DATMXK010000561         2262 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_6573, whole genome shotgun sequence.
ACCESSION   DATMXK010000561 DATMXK010000000
VERSION     DATMXK010000561.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2262)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2262)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2262
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_6573"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..401)
                     /locus_tag="VN363_10230"
     CDS             complement(<1..401)
                     /locus_tag="VN363_10230"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="(2Fe-2S) ferredoxin domain-containing protein"
                     /protein_id="HWQ84935.1"
                     /translation="MSGSNGNQNLALKLEKLREEIIAGRDITRPILTVCAGTGCCASG
                     ALEVRDALEAQIKEQGVDTQVELKITGCPGFCEQGPLVAVLPKHVFYTKVKAKDAAEI
                     VRKTIVNGETVDRLLFKDPVTGQRLVHETDIP"
     gene            complement(398..892)
                     /locus_tag="VN363_10235"
     CDS             complement(398..892)
                     /locus_tag="VN363_10235"
                     /inference="COORDINATES: protein motif:HMM:NF013426.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(P)H-dependent oxidoreductase subunit E"
                     /protein_id="HWQ84936.1"
                     /translation="MAVEIGKIDTIIDKFSAERSGLIPALLGIQDEYHYLPPEALDRV
                     VEKMNIPMIQVQQVAEFYKVFSLEPRGKHIVTVCLGTACHVQGGDRLVDELGRQLSIK
                     PGGTTPDMEFTLETVNCLGACSLGPVMTVDGKYYGHMAANKAEKLLAKFRSEGEEKEE
                     EVAA"
     gene            complement(943..>2262)
                     /locus_tag="VN363_10240"
     CDS             complement(943..>2262)
                     /locus_tag="VN363_10240"
                     /inference="COORDINATES: protein motif:HMM:TIGR02486.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="reductive dehalogenase"
                     /protein_id="HWQ84937.1"
                     /translation="SLGSGGLLSACSSEPGGAANASVTPTRASNVSDYKPAPTFTPQP
                     EVEWIGRTVPTPTPRGSYTPLWYCGACGKQFSSLDMFLTHAASEHVWRLPEIKRVDQP
                     TYTQYIVGEVERFDERNTAFSRRMWDEDYMKLTAETPMKPPRDALAALEGQAVVAGAI
                     YVDNKVGTLHENYYGYNGHVRGAGGLYDWDEPVNPNRYPIDDPLAMTERVKEVARIYG
                     ANLVGVTKLNQRWVYTHYYEQVTGKYGRLTVPFQYAIVMAVEMDWKMINESPGYEASA
                     ATALGYSHMAELSASMAAYIRALGYPATPHGNDTAQSVPLAVDAGLGEGGRHGLLITP
                     EYGTRQRLCKVFTNLPLVPDKPIDFGIQSFCETCHSCSSACPVGAIRSGDRTTEITSI
                     CNRKGLLRWPVDTARCLLFWKENGVECSNCVASCPWSIQSSRDWLDL"
BASE COUNT          450 a          696 c          590 g          526 t
ORIGIN      
        1 gggatatcgg tttcatgcac caggcgctgg ccggtgaccg ggtctttgaa caacagccgg
       61 tcaactgtct cgccgttgac gatcgttttg cggacaattt ctgccgcgtc ttttgctttt
      121 actttggtat agaaaacatg ctttggcagg acagccacta acggtccttg ttcgcagaaa
      181 cccgggcagc cggtaatttt taattccacc tgggtatcca cgccctgctc tttgatttgc
      241 gcttccaggg catcccgcac ttccagagcc ccagacgcac agcaccccgt acctgcgcaa
      301 accgtaagga tcggccgggt gatatcacgc ccggcgatga tctcctcacg cagcttctct
      361 aatttcaagg ccaggttctg gttcccattg ctaccactca tgcagccacc tcctcttctt
      421 tttcttcacc ttcactgcga aacttggcca gtaatttttc ggctttgtta gcggccatat
      481 ggccgtaata cttgccatcc accgtcatca ccggtcccag cgagcaggca cccaggcagt
      541 tcaccgtttc gagggtgaac tccatatccg gggttgtgcc accgggtttg atactcagct
      601 ggcgaccaag ctcatccacc aggcggtccc cgccctgtac atggcaggca gtgcccaggc
      661 agacggtgac aatgtgcttg ccgcgcggct ccaggctgaa taccttatag aattcagcca
      721 cctgctgcac ctggatcatg gggatgttca ttttttccac cacccggtcc agagcttccg
      781 gcggcaggta atgatattca tcctggatac ccagcagagc agggatcaaa ccactccgtt
      841 cggcgctgaa tttatcgatg attgtgtcga tctttcctat ttctactgcc attgggtcgt
      901 acccctttcc tatgaataaa atattgatct gatctaatct gattagagat ccagccaatc
      961 gcgggaggat tggatagacc agggacagga tgcgacgcag ttggaacatt ccacgccgtt
     1021 ttccttccag aacagcaaac accgggcggt atccaccggc cagcgcagca aacctttgcg
     1081 gttgcagatc gaggtgattt ccgttgtccg gtcgccactg cgaatcgcac ctaccgggca
     1141 ggcactcgag caggaatggc aggtttcgca gaagctctgg atcccgaagt cgatcggctt
     1201 atccggcacc aggggcagat tggtgaacac cttacacagc cgctggcggg tgccgtattc
     1261 tggcgtgatc agcaagccat gccgcccacc ctcgcccaac ccggcatcga ctgccagggg
     1321 gacgctctgg gcggtatcat tcccgtgggg tgtcgccgga tagcccaggg cgcggatata
     1381 ggctgccatg gaggctgaga gctctgccat gtgcgaatac cccagcgctg tggcggcgct
     1441 ggcttcgtac cccggcgatt cattgatcat cttccagtcc atctcgactg ccatcacgat
     1501 ggcatactgg aatggcaccg tcaaccgccc atatttcccc gtcacctgct cgtaataatg
     1561 cgtatacacc cagcgctggt tcagctttgt caccccgacc agattggccc catagatgcg
     1621 ggccacttct ttgacccgct ccgtcatcgc caggggatca tcgatcgggt acctgttcgg
     1681 gtttaccggc tcgtcccagt cgtacagtcc acctgccccg cgcacgtgcc cgttgtaccc
     1741 ataataattt tcatgcagcg ttccaacttt gttatccaca tagatcgcac cggcaacgac
     1801 agcctgtccc tccagagcag ccagcgcatc ccgtggcggc ttcatgggtg tttcggcggt
     1861 taatttcatg tagtcttcat cccacatgcg ccgcgaaaag gctgtgttgc gctcatcaaa
     1921 acgctcgacc tcaccgacaa tgtattgggt gtaggtgggc tgatccaccc gtttgatttc
     1981 aggcagcctc cagacatgtt cgctggctgc atgggtcagg aacatatcca ggctggaaaa
     2041 ctgcttccca caggccccac aataccataa tggggtataa gacccgcgcg gggtcggggt
     2101 aggtaccgtc cgcccgatcc actccacctc aggctgggga gtaaatgttg gcgccggttt
     2161 atagtcactc acattggacg ctcgagtcgg tgtaaccgag gcgtttgctg cgccacctgg
     2221 ctccgagctg caggctgata ggagccctcc gctacccagc ga
//
LOCUS       DATMXK010000562         2057 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_85956, whole genome shotgun sequence.
ACCESSION   DATMXK010000562 DATMXK010000000
VERSION     DATMXK010000562.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2057)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2057)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2057
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_85956"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..343
                     /locus_tag="VN363_10245"
     CDS             <1..343
                     /locus_tag="VN363_10245"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558808.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="V4R domain-containing protein"
                     /protein_id="HWQ84938.1"
                     /translation="AGAGDLAFKVLPLSAKMRIGIPAMAKIFSQISDQYSTVQEREHE
                     FIYTIHRCPVCWGRSGSDKPVCFIATGLLQEGLKWLSGGSEFRVNESLCVAMGDLVCE
                     FVIQKQPVSTP"
     gene            453..1190
                     /locus_tag="VN363_10250"
     CDS             453..1190
                     /locus_tag="VN363_10250"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558810.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="polyprenol monophosphomannose synthase"
                     /protein_id="HWQ84939.1"
                     /translation="MQLTVVIPTYNEVENLGKLVSALFALPVPEISILVVDDNSPDGT
                     GDLADSLAQAHSGHFSVLHRQGKLGFGSAYIEGFRAALDSGAEAIAQMDADFSHPPEL
                     LMTLLELLNCCDVVLGSRYVPGGAVDVAWPAWRKGLSAFGNLYARSILRLPVRDATGG
                     FRLWHRRSLLNMPLERVRSNGYAFQIEMAYIASRLGYNFREVPFYFADRRWGQSKMSL
                     RIQIEAAIRVWQMLFEYSDIHPISARP"
     gene            1150..>2057
                     /locus_tag="VN363_10255"
     CDS             1150..>2057
                     /locus_tag="VN363_10255"
                     /inference="COORDINATES: protein motif:HMM:NF019310.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MFS transporter"
                     /protein_id="HWQ84940.1"
                     /translation="MSTATSTPFPLDPNRKANYFHLYADVIGFGILAGSSVAFIAVYA
                     ARLGATAIQLSLLTAGPAVINLLFSLPAGQWLEGRSLTKVSFQAAALSRLGYLLLIPL
                     PLLLPNLTQVWIIILITLIMSIPGTVLSIAFNAILADLIPPDDRATVVGRRSALTSLA
                     MTLAVLLSGQLLDILNYPVNYVIVFSLGTIGGFFSTTHLKKLRVSYTNPPHPAQLPDN
                     PVRRGRIRLLDSLHLPAGLRSLPHRHSAPLLRLDVLRGSFGVFLTAYLFFYTCQFVPV
                     AIAPLYIVNNLEYTDGIISLGNALFF"
BASE COUNT          425 a          558 c          512 g          562 t
ORIGIN      
        1 ggcaggcgca ggggacctgg catttaaagt tctgccgctc tcggcaaaaa tgcggattgg
       61 cattccagca atggcgaaga tattcagcca gatcagcgac cagtacagca ccgtacagga
      121 acgtgagcac gaatttattt ataccatcca tcgctgtccg gtttgctggg gtcggagcgg
      181 ttcggataaa ccggtctgtt ttatcgcaac cggcttactt caagaaggtc tgaaatggct
      241 ttccggaggc agtgaattcc gggtgaacga atccctgtgt gtagcgatgg gtgatctcgt
      301 ttgtgagttc gtcatccaga aacagccggt cagtacgccg taaatttggt catcaacggc
      361 tgtccctcac gtctgcaagg ctccacagca agaaaaatac ctgcgctggc ttgcctgaga
      421 tatggcacac cggatacttt tcagagggta acgtgcagct gacagtagtc attcctactt
      481 acaatgaggt ggagaacctt ggtaaactgg tctccgcttt atttgcttta cctgtcccag
      541 agattagcat tctggtggta gatgataaca gcccggatgg gacaggagat ctggcagatt
      601 ccctggctca agcacattct ggtcatttta gcgtcctgca caggcagggt aagctgggtt
      661 tcggttcagc ctacatcgaa ggttttcgcg ccgcgctgga ttctggtgcg gaagcaatcg
      721 cccaaatgga tgcagatttc tcccacccac ccgaactgct gatgacctta ttggagttgc
      781 ttaattgttg tgatgttgtg ctgggttctc gctatgtacc aggcggggct gtcgatgtag
      841 cctggcctgc ctggcgcaaa ggtctgtccg cgttcggcaa tctctatgcc cgatcaattc
      901 ttcggctgcc cgtgcgggat gcaactggtg gctttcgcct ctggcaccgg cgcagcctac
      961 ttaatatgcc cctggagcgg gttcgctcga atgggtatgc atttcaaatt gaaatggcgt
     1021 acatcgccag ccgtctaggt tacaatttcc gggaagtgcc gttctatttc gccgaccggc
     1081 gctggggaca atcaaaaatg tccctgcgaa ttcagattga agcagctata cgcgtctggc
     1141 agatgttatt tgagtacagc gacatccacc ccatttccgc tagaccctaa ccgcaaagcc
     1201 aattattttc acctgtatgc cgatgtcatt ggttttggca ttctggctgg aagttctgtt
     1261 gcttttatag cagtttatgc agcccgcctg ggagccaccg ctattcaact cagcctgctt
     1321 accgctggac cggcggtgat taacttattg ttttcattac cagccgggca gtggttggaa
     1381 ggacgctcgc tcactaaagt tagcttccag gcagccgccc tatcccggct tggttacctg
     1441 ctgcttatac ctttacccct gctcctaccc aatctcaccc aggtgtggat catcattttg
     1501 atcaccctga tcatgtcgat ccctggcaca gttttatcca tcgcatttaa tgccatcctg
     1561 gctgatctca ttccacctga tgatcgcgcg accgtcgtcg gacggcgcag tgccctcacc
     1621 tcccttgcta tgaccctggc ggtcctgctt tccgggcaat tgctggatat tctaaattac
     1681 cctgtaaact atgtcatcgt tttcagcctg ggcacgatcg ggggcttctt ctccacgact
     1741 cacttaaaaa aactcagagt gtcctatacc aatcctcccc acccagctca gctgcctgac
     1801 aatcctgttc gccggggacg aatcaggtta ctcgatagcc tgcacttgcc ggctggttta
     1861 cgctccctgc ctcaccgaca ttctgcacct ttgctccgcc tggatgtact caggggatcg
     1921 ttcggagtgt ttctgacagc gtatttgttc ttttatacct gccagtttgt tcctgttgcc
     1981 atagcaccgc tgtatatcgt caataatctg gaatacaccg atggcataat cagtttagga
     2041 aatgccttgt tctttat
//
LOCUS       DATMXK010000563         2380 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_52262, whole genome shotgun sequence.
ACCESSION   DATMXK010000563 DATMXK010000000
VERSION     DATMXK010000563.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2380)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2380)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2380
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_52262"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..366
                     /locus_tag="VN363_10260"
     CDS             <1..366
                     /locus_tag="VN363_10260"
                     /inference="COORDINATES: protein motif:HMM:NF015047.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0018580 - nitronate monooxygenase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="nitronate monooxygenase"
                     /protein_id="HWQ84941.1"
                     /translation="VVALLEAAFSELPIILRLPLDRAVELAYSLGPYLDDSRVTAISL
                     GPPRGVVRLPEDGFLRGRQYGPAVLPAALAAVCALSQGSLPVIGAGGIYRQQDIEAML
                     AAGAAAVQVDSVLWRGGID"
     gene            complement(403..840)
                     /gene="dtd"
                     /locus_tag="VN363_10265"
     CDS             complement(403..840)
                     /gene="dtd"
                     /locus_tag="VN363_10265"
                     /EC_number="3.1.1.96"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559364.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0008152 - metabolic process [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="D-aminoacyl-tRNA deacylase"
                     /protein_id="HWQ84942.1"
                     /translation="MRIVLQRVRQGRVSVEEQVVAQIERGLVILLGVGPGDGREQIDY
                     LTEKIVHLRIFEDELGKLNRSIVDIQGQAIVVSQFTLYADTRKGRRPSFTEAALPELA
                     RPLVEAFAERLRSLGVPTQTGVFGAHMLVEIANDGPVTILLER"
     gene            complement(932..1930)
                     /gene="trpS"
                     /locus_tag="VN363_10270"
     CDS             complement(932..1930)
                     /gene="trpS"
                     /locus_tag="VN363_10270"
                     /EC_number="6.1.1.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558541.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005737 - cytoplasm [Evidence IEA];
                     GO_function: GO:0004830 - tryptophan-tRNA ligase activity
                     [Evidence IEA];
                     GO_process: GO:0006436 - tryptophanyl-tRNA aminoacylation
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tryptophan--tRNA ligase"
                     /protein_id="HWQ84943.1"
                     /translation="MTIVKPKGRVFSGARPTGRQHLGNYLGAIQNYIALQEDYECIYC
                     IVDLHALTTVETTGELRQNTYEMALDWLAAGIRPEDAIIFVQSHVPEVTELHTILSMV
                     VPLGKLTDLPTFKEKVRQHPNNINYGLVGYPVLMAADIILYKADTVPVGVDQAPHIEF
                     TREVVRSFNYRYETNVLVEPSMKATSVPKVMGIDGQSKMSKSLDNHIELAATPEETTK
                     RVMQMVTDPQRIRRSDPGNPDVCNVFSMHKIFSTPEQVAEVNVECRRAGIGCVQCKQL
                     YARNLNDHLAPFRQRRAEFDQKPDLVWDVLHHGRDRAKVIASQTMQEVHRAIGLAE"
     gene            complement(1917..2123)
                     /locus_tag="VN363_10275"
                     /pseudo
     CDS             complement(1917..2123)
                     /locus_tag="VN363_10275"
                     /inference="COORDINATES: protein motif:HMM:TIGR02174.1"
                     /note="incomplete; partial in the middle of a contig;
                     missing N-terminus; Derived by automated computational
                     analysis using gene prediction method: Protein Homology."
                     /pseudo
                     /codon_start=1
                     /transl_table=11
                     /product="Rdx family protein"
BASE COUNT          514 a          691 c          618 g          557 t
ORIGIN      
        1 gtggtggcat tgcttgaagc cgctttcagc gagctgccca tcatcctgcg cctgccgctc
       61 gaccgggcgg tagaattggc ttacagcctc ggcccgtatc ttgatgattc ccgcgtgacc
      121 gcgatcagtt tgggacctcc ccgcggcgtc gttcgtctgc cggaggatgg ctttttacgc
      181 ggccggcagt atggtcccgc ggtcttgccc gctgccctcg ccgcggtctg tgccctcagc
      241 caaggcagcc tgccagtgat cggggcggga ggcatctacc gccagcagga tatcgaggcc
      301 atgctggcag ccggcgctgc tgctgtacag gtagatagcg ttttatggcg tgggggaatc
      361 gattaaaagt aactggccgg cgaaattcgc cggccccgga ttctagcgtt ccagcaagat
      421 tgtcaccggc ccatcgttgg caatttcgac cagcatatgt gccccaaaaa cgcctgtttg
      481 agtcggcact cccaggctgc gcaggcgttc agcgaacgcc tccaccagcg gtcgggcaag
      541 ctctggcaga gcagcctccg tgaaggatgg gcgccggcct ttacgggtat cggcatacag
      601 tgtaaattgg gacaccacga tcgcctgccc ctggatatcc acaatcgaac gattcagctt
      661 ccccagctcg tcctcaaaga tacgcaggtg gacgattttc tcggtcaggt aatctatttg
      721 ttcccgccca tcccctggac ctactcccaa taaaatgacc aggccgcgct cgatctgagc
      781 gacaacctgc tcttctacac tcacccgccc ctggcggacc cgctgcagaa ctatacgcat
      841 ctggagtgat tggcctgatt atttcgccag caacattatt cagcgagccg agcttaatta
      901 gccagctgag tttttttcag cgagctgagt ctcactcagc taaccctatt gcccgatgga
      961 cttcttgcat ggtctggctg gcgatgacct ttgcccggtc gcgaccatga tgtaagacat
     1021 cccaaaccag atccggcttc tgatcaaact cagcccggcg ctgccggaat ggcgccagat
     1081 gatcattgag atttcgggcg tacagctgct tgcactgcac acagccgatt cccgccctgc
     1141 ggcactccac attgacctca gcaacctgct cgggggtgga aaagatctta tgcatcgaga
     1201 agacattgca tacatccggg ttgccggggt ccgaacgacg gatgcgctgt gggtcggtaa
     1261 ccatttgcat cacccgcttt gtggtctctt caggtgtggc agccagctca atgtgattat
     1321 cgagactttt actcatctta ctctgaccat cgatgcccat tactttaggc accgaggttg
     1381 ctttcatgct gggttctacc agcacatttg tctcataacg gtagttgaaa gaacgaacca
     1441 cttcccgagt aaactcaata tgcggagcct ggtcaacacc taccggcacg gtatctgctt
     1501 tatacaggat aatatccgcc gccatcagca ccggatatcc caccaaacca taattgatat
     1561 tattcgggtg ctggcggact ttctccttga atgtgggcag atcggtcaat tttcccagcg
     1621 gaaccaccat ggagaggatc gtatgcagct cggttacttc cgggacatgc gattgcacaa
     1681 agatgatcgc gtcttcaggg cgaatgccag ccgccagcca atccagagcc atttcgtaag
     1741 tgttctggcg cagttcgccg gtggtctcca ccgtggtcag ggcatgcaga tcaacgatgc
     1801 agtaaatgca ttcataatct tcttgcagag cgatatagtt ctggatcgcc cccagataat
     1861 tgcccaggtg ctggcgtcct gttgggcgcg ccccggaaaa tacccggcct tttggtttaa
     1921 cgatcgtcat cttgcctccc gagaatttca ccgaccatcc tggcgatctc gcctgggttt
     1981 ggatggcggt ggttttgtaa tttcgaatag atcagcttgc cattgacccg gagctcaaaa
     2041 cgcccgccat cggatggttt gagcacgatt tgttcaatct ccgcttcata cagcttcaac
     2101 aactccgcca ccagactggt agcgcggtcg gtgtagtgtc agactctgca gtactcaagg
     2161 tcaattttta cagccatgct aaagctccaa aggacagatg caaagctaat cgccgcttat
     2221 tgtaccctat tccaaccgaa gcctcgcggg tagcattaat gctcgaatat gctacaatca
     2281 aatgagttac aacgtccatg ccgggcaaaa cgttcaacca gaccccgctg gaacaaacgg
     2341 ggagaagtga gccgagattc gtatgcaaac acaattgggt
//
LOCUS       DATMXK010000564        10194 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_97507, whole genome shotgun sequence.
ACCESSION   DATMXK010000564 DATMXK010000000
VERSION     DATMXK010000564.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 10194)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 10194)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..10194
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_97507"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..898)
                     /gene="rsgA"
                     /locus_tag="VN363_10280"
     CDS             complement(<1..898)
                     /gene="rsgA"
                     /locus_tag="VN363_10280"
                     /inference="COORDINATES: protein motif:HMM:TIGR00157.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005525 - GTP binding [Evidence IEA];
                     GO_function: GO:0043022 - ribosome binding [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ribosome small subunit-dependent GTPase A"
                     /protein_id="HWQ84944.1"
                     /translation="MSNVTLRGRIIKSQSGFYAVQTADGVFVSQLRGRLKRGPRLGDV
                     VAVGDWVHISDLGGGNAVIETIEPRQRMISRLDPRPQGVYQQILIANPDQAFFVFACQ
                     NPEPHLGMVDRFLVIAEKQNLPAVIIANKVDLVGLEAARSIFGHYEPLGYPVIYTSVK
                     TGEGVQALRDSLIGNVSLFSGPSGVGKSSLLNAIQPGLRLSASHVSEATGKGRHTTVV
                     RELMPVIGGGYVADTPGLKALALWDIEPEELDGYFPELRAIVDQCAFSDCTHVHEPDC
                     AVLSALEHGQIHPERYASYLKMR"
     gene            complement(905..3124)
                     /locus_tag="VN363_10285"
     CDS             complement(905..3124)
                     /locus_tag="VN363_10285"
                     /inference="COORDINATES: protein motif:HMM:NF013100.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008658 - penicillin binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="penicillin-binding transpeptidase
                     domain-containing protein"
                     /protein_id="HWQ84945.1"
                     /translation="MKRYIWAMMSLLLILSACSQPVTQPGSTPVEVTATLAEAGVRTT
                     SVPDVKEQARRYLDAWKSEDYAAMYQMLTRVSQEAITEEEFTNQYRSVAAEAGMSSLD
                     YEILSALTNPDTSQVSYRVKLTSILVGNLEWETVMSLGLERGEWHIKWDDTLIHPQLV
                     GENYLRMDLEIPARANIYDQEGKALVAQADATAIGLYPDNILEDQADDLFSWLTRLTG
                     YRSDTIQAMYENFPAGGGWYLPLGEVAASEIARNYDILSGFSGLVLSPYKARYYFDGG
                     IAPHVVGYVSAIQPEEVDFYRRQGYLQDERVGRSGLEYWGEQYLAGKRGGTLYVFDGQ
                     GQPVTRLAESRSQPSQAIYTTLERDFQLAVQQSLGDLVGAIVVLERDTGRVLAMASSP
                     GFDPNAFEPINYNSFTLLSDLNSLNQPLYNRATQGQYPLGSVFKVITMAAALESKLYT
                     AETTYQCGHTFTELPGVTLYDWTLEKEFPPSGLLTLPGGLIRSCNPFFYHIGLDLYNQ
                     NLKSAVSDMARNFGLGQLTGIEGVAELAGQVPDPGSEMDATNLAIGQGTLQVTPLQVA
                     DFVAALGNGGILYRPQVIERIAPPDGSDTITFEPIERGKLPVSEETLQIIRQAMIGVV
                     SSTDPRGTAYHVFSGLNIAVAAKTGTAQSGSGLPHAWFAGYTFEESENKPDIAVAVVV
                     ENIGEGSDYAAPIFRRVVEYYFRGSPGRLYPWESTYDVTRTPTPLFEETPDPGDGGG"
     gene            complement(3166..3843)
                     /locus_tag="VN363_10290"
     CDS             complement(3166..3843)
                     /locus_tag="VN363_10290"
                     /EC_number="3.6.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF014591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="Maf family protein"
                     /protein_id="HWQ84946.1"
                     /translation="MDLILASNSPRRKQILALGGWEFRIQVGEVDEQLLPGEDPRQYV
                     LRLAELKAQAAAREAGTGSCVIAADTTVVDDNQETAILAKPADAEDAAAMLRQLCGRT
                     HQVLTGLVVINLSSAGIAIARDVVATEVQMRQYTDAEIQAYVATGDPLDKAGAYAIQH
                     TGFHPVERINGCYANVVGLPLCALTRRLAEFGILPQSVIARKCQAAADGDPCAVAPFV
                     MEGSLNR"
     gene            3998..4570
                     /gene="xpt"
                     /locus_tag="VN363_10295"
     CDS             3998..4570
                     /gene="xpt"
                     /locus_tag="VN363_10295"
                     /EC_number="2.4.2.22"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560933.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000310 - xanthine
                     phosphoribosyltransferase activity [Evidence IEA];
                     GO_process: GO:0046110 - xanthine metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="xanthine phosphoribosyltransferase"
                     /protein_id="HWQ84947.1"
                     /translation="MQLLKERILKEGRNLGNGILKVDGFINHQVDPALMDLCGQELAR
                     RFANIGATKVLTAEISGIAPALTTALHLGLPVVYARKHKPITMPDQVFLTLTPSHTKG
                     RTVELIISPEYLAGGEKVLIIDDFLASGATILGLIRLAKSAGAQVVGVGALVEKSFEG
                     GRQALEHLGVPIESLACIVQMENDQIIFKD"
     gene            4575..6134
                     /gene="guaA"
                     /locus_tag="VN363_10300"
     CDS             4575..6134
                     /gene="guaA"
                     /locus_tag="VN363_10300"
                     /EC_number="6.3.5.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560932.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003922 - GMP synthase
                     (glutamine-hydrolyzing) activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016462 - pyrophosphatase activity
                     [Evidence IEA];
                     GO_process: GO:0006177 - GMP biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="glutamine-hydrolyzing GMP synthase"
                     /protein_id="HWQ84948.1"
                     /translation="MANSIAILDFGSQTAQLIARRVREMHVYCELFPWDADPAKVLEI
                     NPQGFILSGGPASVYEPGAPYLPGYVLDSKLPILGICYGMQALTHALGGVTAASSKRE
                     YGQATIEKTIDNPLIEMPNLDVWMSHGDRIESPPSGFSVLASSSNSPIAAMGDTQRGY
                     YGLQFHPEVRHTPRGPELLSRFVLDICQISPDWTPDSIINDSVARIRQQVGDQMVISA
                     VSGGVDSSVATALVQRAIGDQLVAVFVDTGMLRENEAAQVQKALHELMGIELITVNAV
                     ERFMGALTDVTDPEQKRRIIGEQFIRVFEEQARSVGEPRFLVQGTIYPDVVESSAPDR
                     AQAQRIKTHHNVGGLPEEMQFGLVEPLRYLFKDEVRLVGEALGLPAELVWRQPFPGPG
                     LAVRCLGEITPERIARLRTADAILVGELREAGLLRLKQQAGSAQGTAQAFAVLLPVRS
                     VGVMGDHRTYQEAIALRVITTEDFMTADWARLPYELLARIANRIVNEVDGVNRVVYDI
                     TSKPPATIEWE"
     gene            6189..7103
                     /locus_tag="VN363_10305"
     CDS             6189..7103
                     /locus_tag="VN363_10305"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84949.1"
                     /translation="MQISEVLSKAWNIIWKHKVLWIFGILVGFSGGNTTSSYTQITER
                     QPVPGQFENFFSQFEGWQIALFILFAVLVVVLLTVLVVFLGTMGRIGLIRGASQADRG
                     ATKLTFGELFNGGLPYFWRIFGLNLIVSLAFAIAIILILLISIPLAITIVGLLCVVPM
                     LLLLAPLGFLVNVVVEQSNNAIVIEGKSMMDGLRRGWDVVSTNLGQTILLGLILMLIS
                     WGVGFVIGLPYALALAPWIASMLTGADISLSSAPAITIIFMVAYLPIILFLNGILQGY
                     IRSAWTLAFLRFTSIRSAILEPNSSPVE"
     gene            7341..9149
                     /locus_tag="VN363_10310"
     CDS             7341..9149
                     /locus_tag="VN363_10310"
                     /inference="COORDINATES: protein motif:HMM:NF028007.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0051219 - phosphoprotein binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="FHA domain-containing protein"
                     /protein_id="HWQ84950.1"
                     /translation="METYLDVHTAGGMFMHGLQPNQIVLLEDEQPVRNVNLTEISPGA
                     QLVVAINPGSEIALRNSQGVSRYDLIYQALAQWTFARAGSSIDDLSLLVTDGRSANHL
                     SDPEAWLAALPTALTDARQAVPSLDNLSQAVDLAADPSPRAGMGRAVLWITPPLENNQ
                     NLALADMASRAIQQGINIYIWLVTASDTQATNRNEPLIALATQTGGQFFQFTGQETLP
                     DLETYVEPHRNIYYLTYTSQVRQSGTHEISVEIHGEVETISTRPQSYTIDLQAPNPAF
                     INPPLSLTRQPPEELTGVAAADVPIADYSPSELDLQILVDFPDQRMRGLVRTALWVDG
                     VVVDENTAPPFDIFQWDLRTVSQSGPHTLQVIAEDVLGLSGSSMNTLIEINVERPANS
                     FWVLLARNTPILIGLAVLLTGAVAFLLMVVGGRIHPRRLPSIRRRGTKPAPAPPAASQ
                     TDADASQAWTGRLHWPQRRIKPQAEAYLTSLATEEAPGSAPPFALTASEITIGSDPQR
                     ATLVLSDPSVDGLHARVARNEAGAYILTDAGSTAGTWINFCLLPPDGICLEHNDLIHI
                     GRSRFRFTLAHPAHIRKPVIRIQDQADPLPPPEDPK"
     gene            9146..>10194
                     /locus_tag="VN363_10315"
     CDS             9146..>10194
                     /locus_tag="VN363_10315"
                     /inference="COORDINATES: protein motif:HMM:NF025053.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="protein phosphatase 2C domain-containing
                     protein"
                     /protein_id="HWQ84951.1"
                     /translation="MIPARRAHLNVTAVSHPGMSGKNNEDRYSVSAFSTPQNPPLPSV
                     LAVIADGIGGHRAGEIAAEIAIETISQVIAASDASAPLHTLGEAIQQASRAIYLQAQQ
                     ATNQRGMGSTCACCWIIGERLYIATVGDSRIYLVRNGAIQQISTDHTWIQEAIDEGEL
                     SPSEARNHPNAHVIRRYLGSRQPAVPDLRLKLNNLENDQQAETNQGFFLLPDDRILLC
                     SDGLTDLVDDQEILRSVQNYPLDTALQQLLVLSNQRGGHDNITIIGLQFPDSASSAPA
                     ALPQKARQNRWIASCMMILAGIAILAVLLIGSFALGVWELGGPIITQTSTSQSSPAPG
                     ILPASATFPIPTRIST"
BASE COUNT         2205 a         2989 c         2688 g         2312 t
ORIGIN      
        1 agcgcatctt gagataagaa gcataccgct cagggtggat ctgcccatgc tcgagcgcac
       61 tcagtactgc acaatccggc tcgtggacgt gagtgcagtc actgaacgcg cattgatcta
      121 cgattgcgcg aagttctgga aaatatccat ccagctcttc cggctcgata tcccataagg
      181 ccagcgcctt caatccgggc gtatcggcta catatccgcc tccaatcacc ggcattaatt
      241 cgcggaccac agtagtatgg cgccctttac cagttgcctc gcttacgtgg gaagccgaaa
      301 gtcttaatcc aggctggatg gcattgagca ggctggattt ccctaccccg gatggcccac
      361 tgaatagaga gacattgccg atcaggctgt cacgcaatgc ctggacacct tcgccagttt
      421 taaccgaagt ataaatcacg gggtagccta acggctcgta gtgaccaaat atcgaccggg
      481 ctgcctctaa tccaaccaga tccactttat tcgcgatgat caccgcgggt agattctgct
      541 tttcggcgat taccaggaag cgatccacca ttcccaggtg aggctccgga ttctggcaag
      601 caaacacgaa gaacgcctgg tcggggttgg caatcaggat ttgctggtat acgccctggg
      661 gtcgcggatc cagccgggag atcatgcgtt ggcgaggttc gatggtttcg atcaccgcat
      721 ttccaccgcc cagatcggaa atgtgcaccc aatctcctac tgcaaccacg tctcccagac
      781 gcggccccct cttcagccgt ccacgcagct ggctgacaaa tacgccatcc gccgtctgga
      841 ctgcataaaa accagattga gatttgatga tccggccgcg taaagttaca ttgctcaaaa
      901 ccggctagcc tcccccatcc ccgggatccg gggtttcttc gaacagcggt gtaggcgtac
      961 gggtgacatc gtaggtagac tcccagggat aaagccgccc tggactgccc cggaaataat
     1021 attcaacaac ccggcgaaaa atcggcgcag cataatcaga gccttcaccg atattttcca
     1081 caaccactgc cacagcaata tccggtttat tttcggattc ttcgaaggtg taacctgcaa
     1141 accaggcatg cggcaagccc gagcccgatt gggcggttcc ggtcttggca gccacagcga
     1201 tattcaagcc gctgaagaca tgatatgccg tgccgcgcgg gtcagtgctg ctgaccacac
     1261 caatcattgc ctggcggatg atctgcaagg tctcctctga aaccgggagc ttcccacgct
     1321 caatgggctc gaaggttatg gtatctgagc cgtccggtgg ggctatccgc tcaatcacct
     1381 ggggacggta taagatcccg ccattcccca acgcagccac aaaatctgct acctgcaagg
     1441 gcgtgacctg tagggtgccc tgtccgattg ccaggttcgt tgcatccatc tcgctgccgg
     1501 ggtccggcac ctgtcctgcc agttcagcca cgccttcaat gccagttagc tgccccagac
     1561 cgaaattgcg cgccatatcc gagacggcag atttcagatt ctggttgtac aggtcgaggc
     1621 caatgtgata aaagaaaggg ttacaggaac ggatcaaacc acccggcaga gtcaacaacc
     1681 cgctgggagg aaattctttt tctaaagtcc agtcatacaa ggttactcca ggcagctcgg
     1741 tgaaggtatg cccacactgg taggtcgtct ctgcggtgta gagcttgctc tccaatgccg
     1801 cagccatggt gatgaccttg aaaactgagc cgagcggata ttgaccctgg gttgcccggt
     1861 tgtacagtgg ctggttgaga ctattcaggt cgctaagcaa agtaaaactg ttgtagttga
     1921 tgggctcgaa agcattgggg tcaaagccgg ggctggaagc cattgccagc acccgcccgg
     1981 tatcccgttc caggaccaca atcgcgccca ccagatcacc cagggattgt tggactgcca
     2041 gctggaagtc gcgttccagg gtggtataaa tggcctgcga gggttgtgag cgcgattctg
     2101 ccaggcgcgt caccggctgg ccctgtccat cgaacacata cagcgttccc ccgcgcttcc
     2161 cggccagata ctgctcgccc cagtattcca gcccgctgcg gcccaccctc tcgtcctgga
     2221 gataaccctg ccgccggtag aaatctacct cttcgggctg gatggcgctc acataaccta
     2281 caacatgcgg ggcaatccca ccatcaaaat aataacgcgc tttgtatggg ctgagcacca
     2341 gcccactgaa accggacagg atatcgtagt tgcgtgcaat ctcactggcg gctacctcac
     2401 caagtggcaa ataccagcca ccgcctgccg ggaaattctc atacatcgcc tggatcgtat
     2461 ccgagcgata accggtcagg cgcgtcagcc agctaaacag atcatccgcc tgatcttcca
     2521 gaatattgtc cggatagagc ccaatcgcgg tcgcatctgc ctgggctacc agagccttgc
     2581 cttcttgatc ataaatattg gcgcgcgccg ggatttccag atccatgcgc agataatttt
     2641 cacccaccaa ctggggatgg atcagcgtat catcccactt aatatgccat tcgccccgtt
     2701 ccaggcccag gctcattacc gtttcccact ccagattccc aaccaggata ctggtcaatt
     2761 taacccggta actgacctgg gaggtgtccg gattagtcaa tgcagataaa atctcataat
     2821 ccaggctgct catcccggct tcggcggcca cactgcggta ttggttggtg aattcttcct
     2881 cagtgattgc ctcctggctc acccgggtaa gcatctggta catggccgcg taatcttcgc
     2941 ttttccaggc gtccagatag cggcgggcct gttcttttac gtctggaacg ctggtcgtac
     3001 gcactcccgc ttccgccagg gtcgcagtta cctcaacggg tgtactcccc ggttgggtta
     3061 ccggctggct gcaggctgac aggataagaa gcagtgacat catggcccaa atatagcgtt
     3121 tcatttttta gattgttttt ctccaatcgg taacctgctc agaggttatc gattcaacga
     3181 tccttccatc acgaatggtg ctacagcaca cggatcaccg tctgcagccg cctggcactt
     3241 gcgggcgata accgattgag gcagtatgcc aaattccgcc aatctgcgcg tcagggcgca
     3301 taatggcagc cctaccacat tggcatagca cccattgatt cgctcaacgg ggtgaaatcc
     3361 tgtatgctgg attgcgtaag ccccagcctt atccagtggg tcgccggtag cgacatatgc
     3421 ttgaatttca gcatcggtat actggcgcat ttggacttcg gttgctacga catcccgcgc
     3481 gatcgcgatc ccggcacttg ataaatttat caccaccagc ccagtgagca cctggtgggt
     3541 acgcccacac agctgccgca gcatggcagc ggcgtcttct gcatctgctg gctttgccag
     3601 aatagcagtt tcctggttgt cgtctaccac tgttgtgtcc gcggcgatca cgcagctccc
     3661 ggtaccagcc tcacgggcgg cagcctgggc tttcaactca gccagccgga gcacatactg
     3721 gcgcgggtcc tcgcccggca ataactgctc gtccacttcg ccaacctgga tgcgaaattc
     3781 ccagccccct aaagccagaa tttgcttgcg cctgggtgag tttgaagcca ggatcaaatc
     3841 catatgccgg attctaacat actcttctac aggtaatttc tactacctta aaatatgccc
     3901 acaggccgac cttcgtcaac actgaaagtt caagaggttg tagaattgaa aaatcgtacc
     3961 ggcgcagggt tatctttaca aaattatgga gtgagtcatg cagttattaa aagaacgaat
     4021 attgaaagag ggtcgtaatc tggggaatgg gatattaaag gtcgatggct tcatcaatca
     4081 ccaggtggat ccagctttga tggatctatg cggtcaggag cttgcccggc gctttgccaa
     4141 tatcggcgcc acgaaggtcc tgaccgccga aatttcaggg attgcccctg ctctgacaac
     4201 ggctctgcat ctgggtttgc cggtcgtata tgcgcgcaag cacaaaccta tcaccatgcc
     4261 ggatcaggtt ttcctcaccc tcactccctc gcataccaag gggcgcactg ttgagctgat
     4321 tatttctccg gaatatctgg cagggggcga gaaggtgctc attattgacg atttccttgc
     4381 cagtggggca accatcctgg gtttaatccg cctggcaaaa tccgccggcg cccaggtcgt
     4441 tggggtaggc gctctggtcg aaaaatcctt cgagggaggc cgccaggcgc tggagcatct
     4501 cggcgtaccc atcgaaagcc tggcctgtat cgtccagatg gaaaacgatc agattatctt
     4561 caaggattga attgatggcg aattccatag ccattcttga ttttggctcg cagactgccc
     4621 agctgatcgc ccggcgggtg cgggagatgc acgtatattg tgaattattc ccctgggatg
     4681 ccgatccggc taaagtgctt gaaatcaatc cccagggctt catcctttcc ggtgggccgg
     4741 cttccgtcta tgagcctgga gcgccctatc tgcccggcta tgtgttggat agtaagcttc
     4801 caatcctggg gatctgttat ggtatgcagg ccctgaccca cgccctgggt ggcgtcactg
     4861 ctgcttcatc aaagcgcgag tatggccagg ctaccatcga gaagacaatc gataatccgc
     4921 tgatcgaaat gcccaacctc gatgtctgga tgtcgcacgg cgaccggatc gagagtcccc
     4981 catctggatt cagcgtgctt gccagcagct caaattcacc catcgctgct atgggcgata
     5041 cccagcgcgg ttactatggg ctgcaatttc accccgaagt ccgccacaca ccccgcggcc
     5101 ctgagcttct gagccggttc gttctggata tctgccagat cagcccggat tggacacctg
     5161 actcaattat taacgatagt gtagcccgca tccgccagca ggtgggcgat cagatggtaa
     5221 tctccgctgt cagtgggggc gtcgattcca gcgtcgccac cgccctggtc cagcgcgcca
     5281 tcggcgatca gctggtcgcg gtctttgtcg ataccggtat gctccgtgaa aatgaggcag
     5341 cccaggttca aaaggccctt cacgagctga tgggcattga gctgattaca gtcaatgctg
     5401 ttgaacgttt catgggcgca ttaacagatg ttaccgatcc tgagcaaaag cgccgtatca
     5461 tcggggagca gttcatccgt gtttttgagg agcaagctcg ctcggttggg gagccgcgct
     5521 ttctggtaca ggggaccatt tatcccgatg tcgttgaatc cagcgctccc gatcgcgccc
     5581 aggcccagcg gatcaaaacc catcataatg taggtgggct gcccgaagaa atgcaattcg
     5641 gtctggttga gccgctgcgt tacttattta aagacgaagt ccgcctggtg ggcgaggcat
     5701 taggtttgcc tgccgagctg gtctggcgcc agcctttccc tggaccgggt ctggcagtgc
     5761 gctgtctggg tgagattacc cctgaacgga tagccaggct gcgcactgcc gatgcaattc
     5821 tggtcggcga gctccgcgag gccggactct tacgtttaaa gcagcaagct ggctccgccc
     5881 agggaacagc ccaggcattc gctgtcttgc tcccggtgcg ctcggttggg gtgatgggtg
     5941 accaccgtac ctatcaagaa gcgatcgcct tgcgcgtgat cacaaccgag gattttatga
     6001 cagccgactg ggcccgcctg ccttacgagc tgctggcacg catcgccaac cgcatcgtaa
     6061 acgaagtcga cggggtcaac cgcgtggttt acgacatcac cagcaaaccg cccgcgacaa
     6121 ttgaatggga atgacgtata ctcgtcatat ggcattcgtc ttgtggacaa tcgaaaggag
     6181 atgcagccat gcaaatcagt gaggttctat ccaaagcatg gaatattatc tggaaacata
     6241 aagtcttatg gatttttggg attctggtgg gcttcagcgg cgggaacact acctccagct
     6301 acactcagat aacagagaga caaccagtcc caggccagtt tgagaatttc ttcagccagt
     6361 tcgagggctg gcaaattgcc ctgtttatcc tgttcgccgt cctggtggtg gtactgctta
     6421 ccgtgctggt cgtattcctg ggtacgatgg gtcgcatcgg attaatccgc ggcgcttccc
     6481 aggcggaccg tggcgcaacg aaacttactt tcggcgagct gttcaacggc ggtctgccct
     6541 acttttggcg gatttttggt ctgaacctga ttgttagcct ggcctttgct atcgccatca
     6601 tattaatttt gcttatcagc atacctctgg caattaccat cgtcggatta ttgtgtgttg
     6661 tcccgatgct gttgttatta gcaccgctcg gatttctggt gaatgtggtt gttgaacagt
     6721 cgaataatgc gattgttatt gaaggcaaaa gtatgatgga tgggctgcgg aggggctggg
     6781 acgtcgtcag taccaacctg ggccaaacca ttttgctggg cctgattttg atgctcatct
     6841 cctggggagt aggattcgtt atcggcctgc cttatgctct cgcccttgcc ccatggatag
     6901 catctatgct cacaggcgct gacatctcgt tgagcagcgc accggcgatc acgatcatct
     6961 tcatggtggc ttatcttccg atcattctct tcctgaacgg gatcctgcaa ggctacatcc
     7021 gctctgcctg gaccctggca tttctgcggt tcacatccat ccgcagtgcc attttagagc
     7081 cgaattccag ccccgtagaa taaacagatc gccatctttg atccagctgt gcctggccaa
     7141 gcaaacgagc caggcacagc tggttgtatt taatcgcgga gttcactttt cctaccttat
     7201 gctacaattt tgctatgtct ttgcgttcca tccggtttag cctatgggcg ctatgtctgg
     7261 tgttgctcgc gtcctctgcg caagcccaat ctacagcccg cgctgaatta acctcgcccc
     7321 aacttggatc ttttccactg gtggaaacct atctggacgt gcataccgcc ggtggcatgt
     7381 tcatgcatgg tttgcagccc aaccagattg tgctcctgga agacgaacag cctgtccgga
     7441 atgtgaacct gacagaaatt agccccgggg cgcagctggt tgtagcaatc aaccccgggt
     7501 cagagatagc actccgaaac agccagggag tctcgcgcta tgacctgatc taccaggcgc
     7561 ttgcccaatg gactttcgcg cgggctggca gcagtatcga tgacttgagt ttattggtga
     7621 cggatgggcg tagcgccaac cacctgtccg atcccgaagc ctggttagcg gcgctcccga
     7681 ccgccctcac cgatgcgagg caggcagtcc ccagcctgga taatttatcc caggcagttg
     7741 atctggcggc tgatccttca ccccgggcag ggatgggtcg cgcagtgttg tggatcactc
     7801 ccccgcttga gaataatcaa aatctggccc tggccgatat ggcatcccgc gccatccagc
     7861 agggcatcaa catctatatc tggttggtca cagccagtga cacccaggca accaacagaa
     7921 atgaacctct catcgccctc gctacccaaa ccggtggaca gttcttccag ttcacgggtc
     7981 aagagaccct gcccgacctg gagacttacg tagagccgca tcgcaacatc tactatctca
     8041 cctatacctc acaggtccgc cagagtggca cccacgaaat cagtgtcgaa atccatggcg
     8101 aggtggagac gatctcaacc cggccccaaa gctatacgat cgacctgcag gcgcctaacc
     8161 cggcatttat aaacccgcct ctcagcctta cccgccagcc ccccgaagaa ctgaccggtg
     8221 tggctgccgc agatgtcccg atcgccgact attctcccag tgagctcgac ctgcaaatcc
     8281 tggtggattt cccggaccaa cgtatgcgtg gtctggtgcg aaccgcgttg tgggtcgacg
     8341 gcgttgtcgt tgacgaaaat actgctcccc ccttcgatat tttccagtgg gatttacgca
     8401 ccgtctcgca aagcgggccc catacgttgc aggttattgc agaagatgtg cttgggctca
     8461 gtggttccag tatgaacacg ctgattgaga taaatgtcga gcgtcctgcc aacagcttct
     8521 gggtgctcct ggcgcgcaat acacccatcc tgatcggtct ggccgtcctg cttaccggtg
     8581 ccgtcgcgtt tttactgatg gtcgttggtg ggcggattca tccccgccgg ctgccgagta
     8641 tccgccggcg aggcacaaaa ccagccccgg caccaccagc tgccagccaa accgacgcgg
     8701 acgcttctca agcctggacg ggccgcctgc attggcctca acgccggata aaaccccagg
     8761 cagaagccta cctgacttcc ctggcaaccg aggaggcacc cggaagtgct cctcctttcg
     8821 cattgaccgc ctcggaaatc acgattggca gcgatcctca acgggctacc ctggtgttaa
     8881 gtgatccctc ggtcgatgga ttacatgccc gggtggcaag aaacgaggct ggtgcatata
     8941 ttctgaccga tgctggctca accgccggaa cctggatcaa cttctgtttg cttcccccgg
     9001 atggtatctg cctggaacac aatgatctga tccatatcgg tcgcagcaga tttcgtttca
     9061 cactcgccca tcctgcccac atccgcaagc cggtcattcg tatccaggat caagcagatc
     9121 cgcttcctcc gccggaggat ccgaaatgat cccagccagg cgagcccatt tgaatgtaac
     9181 ggctgtcagc catcccggga tgagcggtaa gaacaacgaa gaccgctaca gtgtatcggc
     9241 gttttcaact ccgcagaacc ctccgctgcc cagcgtgctg gcagttattg ccgatgggat
     9301 cggcggacac cgcgccggag agattgctgc tgagattgct attgagacta tcagccaggt
     9361 cattgccgcc agcgatgcga gtgcgccact ccatactctt ggggaggcca tccagcaagc
     9421 cagccgggcg atttaccttc aggcccaaca ggcaaccaac cagcgcggca tgggatcgac
     9481 ttgcgcctgc tgctggatca tcggggaacg cctctatatc gcaacagtgg gcgactcacg
     9541 catctacctt gtccggaatg gagccatcca acaaatttca actgatcaca cctggattca
     9601 agaagctatc gacgagggtg agctgtcgcc gagcgaggcg cgaaatcatc ccaacgcgca
     9661 tgttatccgc cgttatctgg gctcacgcca gccagcggtg ccggatctac gcctcaagct
     9721 gaacaatctc gaaaatgacc agcaagctga gaccaaccag ggattcttcc tgctgccgga
     9781 cgaccggata ctgctctgtt cggatggttt gaccgatctg gtagacgatc aggaaatcct
     9841 gcggtctgtc caaaactatc ccttggatac tgccttgcag cagctgttgg tgttatccaa
     9901 tcagcgcggc gggcacgaca acataacaat catcggactg cagtttcctg attcagccag
     9961 cagtgcgccg gcagccttgc cgcaaaaggc acgccagaac agatggatag ccagctgtat
    10021 gatgattctg gccgggattg ccatcctggc agtcctgtta atcggtagtt tcgccctggg
    10081 agtctgggag ctgggcggac caatcattac tcaaacctca acctcccaga gctctcctgc
    10141 accaggaatc cttcctgcca gcgctacctt tcccatcccc acccgcataa gcac
//
LOCUS       DATMXK010000565         2517 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_134520, whole genome shotgun sequence.
ACCESSION   DATMXK010000565 DATMXK010000000
VERSION     DATMXK010000565.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2517)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2517)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2517
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_134520"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..122)
                     /locus_tag="VN363_10320"
     CDS             complement(<1..122)
                     /locus_tag="VN363_10320"
                     /inference="COORDINATES: protein motif:HMM:NF024867.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA];
                     GO_process: GO:0006302 - double-strand break repair
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ84952.1"
                     /translation="MLRELRITNFAIIDELELQFIPNLNIFTGETGAGKSIIIDA"
     gene            complement(139..660)
                     /locus_tag="VN363_10325"
     CDS             complement(139..660)
                     /locus_tag="VN363_10325"
                     /inference="COORDINATES: protein motif:HMM:NF012850.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008270 - zinc ion binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="B-box zinc finger protein"
                     /protein_id="HWQ84953.1"
                     /translation="MTNSTPIMVCANHPDTETTLRCNRCEKPICPRCAVQTPTGYRCK
                     ECVRGQQRIFDTSLWYDYPVAILIASILGFLGSLFVPRIGFFAIFIGPIVGSVIAEAV
                     RRITNRRRSRGLFISTTVAAAVGCTIQLLPLFLNALIGGGFGSLLGLLWYGIYTFGVV
                     SSLYYRLSGIRIQ"
     gene            complement(657..1529)
                     /locus_tag="VN363_10330"
     CDS             complement(657..1529)
                     /locus_tag="VN363_10330"
                     /inference="COORDINATES: protein motif:HMM:NF039482.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NAD(+)/NADH kinase"
                     /protein_id="HWQ84954.1"
                     /translation="MPQAQASIQQIAIATPPQMPATLDVAQQVNTCLVETGFRTITGY
                     LHDETLRQLVTDGEIDLLIAVGGDGTMLRAGHLCGPAKVPILGINMGRFGFLIEVQVD
                     QWRKLIPRLKSGEYWLEQRMMLAVAHWRDHSLLGNWDALNEVAVGRSEYLRPVHLSAY
                     LDGRFLTTYVADALIASTATGSTAYALAAGGPILPPELRNILLVPVAPHLSVERAIVL
                     AEGSTIGITVISEHEASCSVDGQPPMTVLKGDRIEARASEHTVQFVRFQDPGYFYRYL
                     TPHMSQNPSTGNLR"
     gene            complement(1580..2104)
                     /gene="cas4"
                     /locus_tag="VN363_10335"
     CDS             complement(1580..2104)
                     /gene="cas4"
                     /locus_tag="VN363_10335"
                     /inference="COORDINATES: protein motif:HMM:TIGR00372.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004518 - nuclease activity [Evidence
                     IEA];
                     GO_process: GO:0043571 - maintenance of CRISPR repeat
                     elements [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="CRISPR-associated protein Cas4"
                     /protein_id="HWQ84955.1"
                     /translation="MSGVLIPLAIVLLLIAGILFWQAGRRQKAAGLPGGRVITSDTRQ
                     WGPVTEPLYDPHHGLTGKPDYLVQQGNLLIPIEVKSSRVSAAPYDAHIFQLAAYCMLV
                     ESVYGKRPPHGILHYPNRTFAIDYTSELESALLDILEEMRTRDRQKDVLRSHEDPGRC
                     RGCGYRQSCDQMIE"
     gene            complement(2108..2353)
                     /locus_tag="VN363_10340"
     CDS             complement(2108..2353)
                     /locus_tag="VN363_10340"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84956.1"
                     /translation="MRTIRASEISTFIYCQRAWWYQKQGIASQNQPQLAAGTSLHLQH
                     GKTVLIAGIFRILAYGLVLLALGLLAAALTQALLRAL"
BASE COUNT          641 a          624 c          679 g          573 t
ORIGIN      
        1 gcatcaataa tgatggactt gcctgcaccc gtttctccag taaagatatt gaggtttggg
       61 atgaattgga gttctaattc atcgataatc gcaaaatttg tgatgcgtaa ttctctcagc
      121 ataagtctaa gttaaagatt attggatgcg aatgcctgaa aggcggtaat aaagtgaaga
      181 aacaactcca aatgtgtaaa tcccgtacca gagcaaaccg aggagggatc cgaacccacc
      241 acctattaag gcattcagga agagtggtag cagctggatg gtacagccca cagcggcggc
      301 tacggttgta ctgataaata agcctcgtga gcgcctgcga ttggttatcc ggcgaacggc
      361 ttcggcaatg acagagccaa caatcggtcc gataaaaatc gcaaaaaaac cgatgcgcgg
      421 cacaaacagg ctgccgagaa aaccaagtat actcgcgatc aggatcgcaa caggataatc
      481 ataccagagg gaagtgtcaa agatccgctg ctggccgcgc acacactctt tacaccggta
      541 acctgtagga gtctgtactg cacagcgtgg gcagatcggt ttctcgcagc gattgcagcg
      601 taaggttgtt tcagtatcgg ggtggttggc gcacaccatg atcggcgttg aattggtcat
      661 cgcaaattac ctgtggaagg gttttgactc atgtgggggg ttaaataacg ataaaaataa
      721 ccaggatcct ggaaacgtac aaactgtacc gtgtgctcgc tggcgcgggc ttcaattcga
      781 tcgcctttaa gaacggtcat tgggggttgt ccatcaacac tgcagctggc ttcgtgttca
      841 gaaatgaccg taattccaat tgtcgagcct tccgcaagta cgatggcgcg ttccaccgac
      901 agatgcggag ctacggggac aaggagaata ttccgcagtt caggcggtaa gattggaccg
      961 cctgccgcca gtgcataagc ggtcgatcca gtggcggtag aggcgattag tgcatcagct
     1021 acataggtgg tcaggaagcg cccgtccagg taagcggaca gatgtaccgg acgcagatac
     1081 tcgcttctcc caacagcaac ctcatttaac gcatcccagt tacccaggag agaatgatct
     1141 cgccaatgcg caacagcgag catcatgcgt tgttcgagcc agtactcgcc gctctttaaa
     1201 cgcgggatta attttcgcca ctgatctacc tgcacttcaa ttaaaaagcc gaagcgaccc
     1261 atattaatcc ccaggatcgg gacttttgca ggtccgcaga ggtgaccggc acgcagcata
     1321 gtcccatctc cacccaccgc aatgagcaaa tcaatttcac cgtcagttac aagctggcgg
     1381 agggtttcgt catgcaggta gcccgttatg gtcctgaagc cagtctctac caggcaagta
     1441 ttaacttgtt gagcaacatc gagcgttgct ggcatctgag gaggcgtggc gatagcaatt
     1501 tgttggatag aggcttgtgc ttgcggcata tcggttaatt atagtgtgaa atctggcaac
     1561 tgtcttaaat aggacaaaat tattcgatca tctggtcgca gctctggcga tatccacacc
     1621 cccggcagcg accaggatct tcatgcgagc gaaggacatc cttttggcga tcccttgtcc
     1681 gcatctcttc cagaatatct aataaggcag attcgagctc ggaggtgtaa tcaattgcga
     1741 aagtgcgatt tggatagtgc aggatgccgt ggggtgggcg tttgccgtac actgattcaa
     1801 ccaacataca gtaggctgcc agctgaaaaa tatgcgcatc gtagggggct gccgaaaccc
     1861 gactggattt tacttcgatc gggatgagca ggttgccttg ttgtacaagg taatcgggtt
     1921 ttccggtcaa accgtgatgt gggtcataaa gcggctcggt gacaggcccc cattgacggg
     1981 tgtcgctggt gattacccgc cctccgggca agccagccgc tttctggcgg cgtcccgctt
     2041 gccagaatag aattccagct atcaggagga gaacgatagc cagggggatc agcacaccag
     2101 acatcgctta gagagctcgc aataaagcct gagtgagcgc agcagccaac aaacccagcg
     2161 ctagcagcac cagaccataa gccaggatgc ggaatatgcc tgcaatcagg accgttttgc
     2221 cgtgctgaag gtgcaaagat gtgccggctg ccagctgcgg ctggttttgg gatgctatac
     2281 cttgcttttg ataccaccag gcccgctggc agtaaataaa ggtgctgatt tcagatgccc
     2341 ggatagtgcg catagctatt gattatttgt tacccagcga ttttaattgc tgctcgacat
     2401 ttgccagact ccgctccaat tgctgctggc ggaggcttaa tgtcagatca ccccgcgtgc
     2461 gttcggtgac tccgcgcaca atatctgtca accggcgcaa gccacctgtg atggcat
//
LOCUS       DATMXK010000566         2384 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_132429, whole genome shotgun sequence.
ACCESSION   DATMXK010000566 DATMXK010000000
VERSION     DATMXK010000566.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2384)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2384)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2384
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_132429"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..1570)
                     /locus_tag="VN363_10345"
     CDS             complement(<1..1570)
                     /locus_tag="VN363_10345"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84957.1"
                     /translation="MKTRILIAGIFVIALLGSAACVSASAPEASSSPSPSAVSGGALA
                     AIPGWQQVNVNGFGDPQTDEVTALEAFNGYVYAGIHNPVDPEPLFDGARIYRSANGES
                     WTAVTQPGFAIAHDIAPPAILDLAVFNTRLYASTGRGDGPGQIWRTQTGTIWSPMVID
                     GFGDPDNVDVTALAVYNSLLYAGVTNLVGGAQIWRSNSGDNNTWTQVAFGMTGTAAAS
                     ISGFVEFGGTLYAAVESEGPVQIWRSDGGDWTAVVSDGFGDNQTLTAGGLAVFGGYLY
                     VGVGNAASGALLMRSNDGLTWDPAISPGFGDPNNNQVDLVFVFQNQLYAGVHNVVTGI
                     ELWRTADGVLWEQANLDGFEDSHNTGSNESNAAADFLNHFYVGTANSFAGGELWRMQQ
                     PYGVVLSPDDTHAGAAGTTVIYNLTVSNTGDVSDTFSLASAGNTWTTLLSTSSIPLAA
                     GASGDFTVSVSIPDGASGGESDMATITATSQVDPTITDSAVLTTTVTTGYAVVLSADQ
                     NAQGLAGQTLSYTLG"
     gene            complement(1816..>2384)
                     /locus_tag="VN363_10350"
     CDS             complement(1816..>2384)
                     /locus_tag="VN363_10350"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="fibronectin type III domain-containing protein"
                     /protein_id="HWQ84958.1"
                     /translation="SALAATVQAGPQVLLTWRDNAVTEPGFIVERSAGGGAFAEIGTV
                     PAFAGSGPGVSFLHTLLGQPLDITYSYRVKALNAGGSSSYSNQINIFVPSVPLAPSNL
                     LASAFLQGNNARVTLTWTDNAFNESAYRVWRSTDPNFATGVTIVNLGANATSYTTGNI
                     ARGTLWYFRVTALNVSGFGSAETSVTTP"
BASE COUNT          494 a          704 c          696 g          490 t
ORIGIN      
        1 ttcccagggt atacgagagg gtctggcctg ccaatccctg agcattctga tcggccgata
       61 gaacgacggc gtagccggtg gttacagtgg ttgtcaggac agcactgtcg gttattgtgg
      121 gatcaacctg agaggtggct gtgatcgtag ccatatcgct ctcaccgcct gaggcgccgt
      181 ctggaatgga aacagagaca gtaaaatcgc cgctggcgcc agccgccagc ggaatgctgg
      241 aagttgagag gagggtcgtc caggtattgc cagcagatgc caggctgaag gtatcgctca
      301 catcgccggt attgctgacc gtcaaattgt agattaccgt tgtcccggca gccccggcgt
      361 gagtatcgtc aggagagagg acaacaccgt agggctgctg catgcgccaa agctcacccc
      421 cggcaaagct gttcgcagta cccacataga agtggttcag aaaatcagcc gcggcattac
      481 tctcattcga tccggtgtta tggctgtcct caaagccatc cagattggcc tgctcccaga
      541 gcacgccatc ggcggtgcgc cacagctcga tcccggtgac cacattgtgt acgccggcat
      601 agagctggtt ctgaaaaacg aacaccagat cgacctgatt gttgttggga tcgccgaagc
      661 cagggctgat cgccggatcc caggtcaagc cgtcgttgga tcgcatgagc agtgccccgc
      721 tggctgcatt tccgaccccg acgtacagat aacccccaaa tacagccaac ccgcctgcgg
      781 tcagggtctg gttatcgccg aagccgtcgc tcaccacagc cgtccagtcg cctccgtcgc
      841 tgcgccagat ctggaccgga ccttccgatt caacagcggc atacagcgtg ccaccgaact
      901 cgacaaagcc ggaaatgctg gcagcagccg ttcctgtcat cccaaaagca acctgagtcc
      961 aggtattgtt atcgccgctg ttgctgcgcc agatctgcgc cccaccgacc aggttcgtca
     1021 ccccggcgta gagcaggctg ttgtatacag ccagggcggt aacatccacg ttatccggat
     1081 cgccgaaacc atcgattacc atcggtgacc agattgtgcc agtctgagtt cgccagattt
     1141 ggcccggtcc atcacccctg ccggtcgagg cgtagagccg ggtattgaag actgccagat
     1201 cgaggatggc aggtggggca atatcgtggg cgatggcgaa tccaggctgg gttacggcag
     1261 tccagctctc tccattggcg gagcggtaga tgcgcgcccc atcgaacagc ggctctggat
     1321 caaccggatt gtggatcccg gcatacacgt agccgttgaa ggcttcgagt gcggtcacct
     1381 cgtctgtctg cgggtcacca aatccgttta catttacctg ctgccagcca ggaatcgccg
     1441 ccagtgcccc tcccgatacg gcggaagggg acggagaact gctcgcttct ggcgcagacg
     1501 cggaaacaca tgccgcactg cccagcaagg cgatcacaaa aatgccagct attaatatcc
     1561 tggttttcat tgtgcctgac tttcccatat acccatctgg atcgcgttca tgcttatcga
     1621 tttccgtgtc cggggcattt ctttaagcct gagatcagcg ttaccccacc gacagcgttt
     1681 atatttttgg ccgaaaacga atttgatcat ccttttgatt tatttgggtc gctttcctgc
     1741 gaccgccagc agcattcgtt ccgccgttgg gggagggatt gctccctccc ccaaacaggg
     1801 ttgaacagcg ggtagttatg gtgtggtcac actggtctct gccgagccaa agccgcttac
     1861 attgagggct gtgacccgga agtaccagag tgtgccgcga gcgatgttcc cggtggtgta
     1921 ggacgtcgcg ttagcgccca ggttgacgat cgtaaccccg gttgcgaagt ttggatcggt
     1981 ggagcgccag acccggtatg ccgactcatt gaacgcgttg tcagtccagg tcagcgtcac
     2041 ccgggcattg ttgccctgca agaaggcgct tgccagcagg ttggagggag ccagcggaac
     2101 gctgggcaca aagatgttaa tctggttcga ataggacgaa gatccacctg cgttcaacgc
     2161 cttcacccgg tagctgtagg tgatatcgag gggctgcccc agcagggtat gcaggaacga
     2221 aacccctggg ccgctgccgg cgaaggctgg aactgtaccg atctcagcga aggccccgcc
     2281 acctgcggac cgctcaacga tgaagcccgg ttcggtcacc gcattgtccc gccaggtcaa
     2341 gagcacctga ggacccgcct gcactgtggc tgccagggcg ctcg
//
LOCUS       DATMXK010000567         3848 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_42692, whole genome shotgun sequence.
ACCESSION   DATMXK010000567 DATMXK010000000
VERSION     DATMXK010000567.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3848)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3848)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3848
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42692"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..212
                     /locus_tag="VN363_10355"
     CDS             <1..212
                     /locus_tag="VN363_10355"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84959.1"
                     /translation="EEIMNLHPDTILFEKTRAGIPVDMLEILEVEAWDMRIIGLSLEN
                     NEMSLYHHEHQTVVAAGDLLQFVLS"
     gene            392..1723
                     /locus_tag="VN363_10360"
     CDS             392..1723
                     /locus_tag="VN363_10360"
                     /inference="COORDINATES: protein motif:HMM:NF024834.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="c-type cytochrome"
                     /protein_id="HWQ84960.1"
                     /translation="MSRKILFLVTFILLGGLMLAACAGPAGPEGPQGPQGPAGPQGSA
                     GPAGADLTEEQTLALETAGNLAAIQFPALDEVRRGCPACHALVDPETGQFTLPFEANE
                     RAEARGKEHPEVAPDGTSLAVNEEVKVTTCLQCHASGTGDRSGKGVIAPLALRDIVHP
                     AHMNSQFFKLHYGGNCFTCHNVNGEGDWDLLTEKVEVNDKGVPSPDALPIPGAIPIGS
                     SEGTSSGTASRGGRLYDNWVTEAGVEIPATDQPLWATQTTNTRTGTDTWRCKECHGWD
                     YLGADGAYGSGSHFTGFVGVTGVASKTSDEIVAILSGGTNSDHDFSIMGEDALADLAT
                     FLQTGLIDFTPLVDAETKSAVGGDAANGEELFATCASCHGADGRVLNFGNEEEPEYIA
                     TIALDNPWEFLHKVRSGQPGTVMPAAMDGDWSLEDLVDLLAYAQTLPVETP"
     gene            1880..2383
                     /locus_tag="VN363_10365"
     CDS             1880..2383
                     /locus_tag="VN363_10365"
                     /inference="COORDINATES: protein motif:HMM:NF025875.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009055 - electron transfer activity
                     [Evidence IEA];
                     GO_function: GO:0020037 - heme binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cytochrome c3 family protein"
                     /protein_id="HWQ84961.1"
                     /translation="MTRRLLLFFSILILAAVLLTRAQVLASPEQPVAFNHQIHAQAGV
                     QCLFCHSSALRADIAGIPSMQKCMGCHTIIATDNVAVQVLASYWERREPIPWVRVSWQ
                     PEFVYFSHQAHLRTGENCETCHGDVGRMPLTRPTVKMDMGWCLDCHLQQPEEKVVRLA
                     DCLTCHK"
     gene            2440..>3848
                     /locus_tag="VN363_10370"
     CDS             2440..>3848
                     /locus_tag="VN363_10370"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016491 - oxidoreductase activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="molybdopterin-dependent oxidoreductase"
                     /protein_id="HWQ84962.1"
                     /translation="MPKTITRREFLKIAAGASVATGFTSAVRSIVIEPFIHPPEEELP
                     GQATWYASTCRQCSAGCGIIVRTIDGRAKKIEGNPLHPLNRGKLCARGQAGLQVLYNP
                     DRLKNAVRQVGGRGSRQFEPFYWPDALDLLSSKIAALSNSVRFAFLGGLMPSHTYRLT
                     SLLLEALGAAPPVIYDLQSALEGRSAVSQLSQALFGAPDLPEYDLSQSDVVLSFGANL
                     LETWMSPVAQSVAYGAMRQGSMGRRGLLVQFEPRLSSTAASADEWIPVKPGAEGFVAL
                     AIGRIIVEERLGHVGSHQSQAVLYQSVDVRDMSAASDVPVDTLRRLARVFADADRAVA
                     IPGGYLAGQQNGFSSMLAVQALNLVIAQVGRQGGMFLSNPGPTAALRQNPPVNTFDEV
                     MDLIRRMKAGEIDLLLLHSANPLYELPAAAGFIEAIARVPFVVSFSPVVDETAVWADL
                     ILPDHTYLESWGYQVPSPGA"
BASE COUNT          880 a         1031 c         1056 g          881 t
ORIGIN      
        1 acgaagagat catgaatctt catcccgaca cgatcctttt tgaaaagacc agagccggta
       61 ttccggtcga tatgctggaa atactggagg ttgaggcatg ggatatgcgc atcatcgggc
      121 ttagcctgga aaacaacgaa atgagtcttt atcaccacga acaccagact gtggtagcag
      181 caggcgactt gctccaattc gttctcagct gacctggcat gtatggacac ctaataaaaa
      241 ttataaggct ggcaagcaag catctgccat gggccagcca gcaccaaacc ggtgaagctg
      301 ccaatatccg agaataaagg aggttacatg gcgtaatgtt caaaggaaat ctgccaacaa
      361 ggctgataaa gaagaatcag gagaatgtga aatgtcaaga aagattctat tcctcgttac
      421 ctttattctg ttagggggcc tcatgttagc agcctgtgcc gggcctgctg gaccagaagg
      481 tccgcaaggg cctcagggtc ctgctggacc tcaaggttcc gctgggccag caggggcaga
      541 tttaacggaa gaacagacgt tagcgctcga aacagctggt aatttagcag cgatccaatt
      601 tccagctctg gatgaggtaa ggcgaggctg ccctgcatgt catgctctag ttgatccaga
      661 gaccggtcaa ttcaccttac cttttgaggc aaatgagcgt gcagaagcca gaggaaagga
      721 gcatccggag gttgccccag acggaacatc gctcgctgta aatgaggagg taaaggtcac
      781 aacctgcctg caatgccatg cgtctggcac tggcgatcgc tctggaaaag gggttatcgc
      841 cccgcttgcc ctgcgcgaca ttgtccaccc cgcccatatg aatagccagt ttttcaaact
      901 ccattatgga ggcaactgtt tcacctgtca caatgtcaat ggcgagggtg attgggatct
      961 actcactgaa aaagtcgagg tcaatgacaa gggtgtaccc agccccgatg ctctacccat
     1021 acccggtgct atacccattg gctcgagtga aggcacctct tcaggaacgg cctcccgggg
     1081 gggcagattg tatgacaact gggtgacaga agctggagta gaaatacctg ctactgacca
     1141 acccctatgg gcgacccaaa ccactaacac ccggaccgga acagatactt ggcgctgcaa
     1201 agaatgccac ggatgggatt acctgggagc agatggcgcg tatggctcag gctcccactt
     1261 taccggcttc gttggcgtaa ctggagttgc ctcaaaaacc tcggacgaga tcgtagcaat
     1321 cctatcgggg ggaaccaatt ccgatcacga cttcagcatt atgggagaag atgccctggc
     1381 tgatcttgcg acattcctcc aaaccggatt gattgacttc acgcccctgg ttgatgctga
     1441 aaccaaaagc gcagtcggcg gtgatgccgc caatggcgaa gaactcttcg ccacctgtgc
     1501 atcctgccat ggcgcagatg ggcgagtgct caacttcgga aacgaggaag agcctgaata
     1561 tatcgctacg atcgctttag ataacccatg ggagttcctc cataaggttc gctccggtca
     1621 accgggaaca gtcatgccgg ctgccatgga tggagattgg agcctggagg atttagtgga
     1681 tctactggct tacgctcaaa ctttacctgt cgagacacca taagggagtc gcaaagtaca
     1741 caaacggagc aggggttgtc caatgagaat atattcttgt tttttcctaa aaccgcgtgc
     1801 aggtcaggct tccatgcctg cctgataggc gcctatttgg acagcccctc cctgtaaatt
     1861 tatctccttt aagaataaga tgactcgccg cttgttatta ttcttttcca tcctgatctt
     1921 agccgccgtg cttctgacgc gtgcccaggt cctggctagt ccagagcagc ccgtcgcatt
     1981 caaccaccag attcacgcac aggccggtgt gcagtgttta ttctgccact ccagtgcttt
     2041 acgcgcagac atcgccggga taccttccat gcaaaaatgc atgggctgcc ataccatcat
     2101 tgccacggat aacgttgccg tccaggttct ggccagctat tgggagcgaa gggagccaat
     2161 tccgtgggtc cgcgtaagct ggcagccgga attcgtttat ttcagccatc aagctcatct
     2221 gaggacaggc gagaattgtg aaacatgtca tggggatgtc ggcaggatgc cgctcacacg
     2281 gcctactgta aagatggaca tgggttggtg tctggattgc catctgcagc aacccgaaga
     2341 aaaagttgtc cgcctggcag actgcctgac ctgccacaaa tgaaccagtt tgtaggaata
     2401 cttaacaaca acgcgttact ttcgacatac agaaagaata tgcctaaaac aatcacccgc
     2461 cgagaatttc tcaagatcgc tgccggggct tctgtagcca ccgggttcac ctcagccgtc
     2521 cgcagtattg tgattgagcc ctttatccat ccaccggaag aagagcttcc cgggcaggct
     2581 acatggtatg ccagcacctg ccggcagtgc tccgccgggt gtgggattat tgtgcgcacc
     2641 atcgatggac gggccaagaa gatcgagggg aatcctttgc atccgctcaa tcgtggaaag
     2701 ctatgtgcaa gaggacaggc tggattgcag gtgttataca accccgaccg gcttaagaat
     2761 gctgttcgcc aggttggagg ccgtggttcg cgtcagtttg agccatttta ctggcctgat
     2821 gcgctggatt tattaagcag taagattgcg gctctaagca actcggtgcg gtttgcattt
     2881 cttggcgggc tgatgcccag ccatacttac cggctgactt ctcttctcct cgaagcgctt
     2941 ggcgcggccc cgcctgtcat ctatgactta caatccgccc tggagggtcg cagtgctgtc
     3001 tcgcagctat cccaggcatt attcggggcg ccagacctgc ctgaatatga tctctcccag
     3061 tctgacgtgg ttctctcttt cggggcgaat ctgctcgaga cctggatgtc tccagtcgcc
     3121 cagagtgttg cctatggcgc tatgcgccag ggaagcatgg gcaggcgggg gctcttagtt
     3181 cagtttgagc cgcgcctttc ctcgaccgcg gcttctgccg atgagtggat cccggtaaag
     3241 ccaggggctg aggggttcgt tgccctggct atcggccgta tcatcgtcga ggagagactc
     3301 gggcacgttg gcagccatca atctcaagca gtcctatatc agagcgtgga tgtgcgcgac
     3361 atgtccgccg ccagcgatgt accggttgac accttgcgcc gtctggcgcg tgtctttgcg
     3421 gatgccgatc gcgccgtggc aatcccaggt ggatatctgg cagggcagca gaatggtttt
     3481 tccagcatgt tggctgtcca ggcgttgaat ctggtgattg cacaggttgg gcgccaaggc
     3541 ggtatgttcc tctcaaaccc gggtcctact gcagcactcc gccagaatcc gcctgtcaat
     3601 accttcgacg aagtgatgga ccttatccgc cgaatgaaag ctggcgagat cgacttgttg
     3661 cttcttcaca gcgctaatcc cctgtacgag ctgccagccg cggccggttt catcgaggct
     3721 atcgcccgcg tgccgtttgt ggtctctttc agcccggtgg tagatgaaac agccgtgtgg
     3781 gcagatctga tcttgccaga ccatacttat ctggaatcct ggggatacca ggtgccctca
     3841 cctggcgc
//
LOCUS       DATMXK010000568         2131 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_120876, whole genome shotgun sequence.
ACCESSION   DATMXK010000568 DATMXK010000000
VERSION     DATMXK010000568.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2131)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2131)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2131
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_120876"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(117..2114)
                     /gene="fdhF"
                     /locus_tag="VN363_10375"
     CDS             complement(117..2114)
                     /gene="fdhF"
                     /locus_tag="VN363_10375"
                     /EC_number="1.17.1.9"
                     /inference="COORDINATES: protein motif:HMM:NF012602.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0009326 - formate dehydrogenase complex
                     [Evidence IEA];
                     GO_function: GO:0008863 - formate dehydrogenase (NAD+)
                     activity [Evidence IEA];
                     GO_process: GO:0015942 - formate metabolic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_except=(pos:complement(1788..1790),aa:Sec)
                     /transl_table=11
                     /product="formate dehydrogenase subunit alpha"
                     /protein_id="HWQ84963.1"
                     /translation="MHLCVKGRYGYDFVHHPDRLTQPKVRKYLLEGDGARPAVKDRGE
                     WVTVDWDTALDITARKLTGYRDTFGSRSVGVLTSAKCLNEENYLMNKFARQVVGTNTI
                     DHCARLUHSSTVAGLATSFGSGAMSNSMEDVAQFAKSMLVIGSNTTEQHPVFGAKIRQ
                     AVLQRGVKLVVADPRKIDLTEFATLHLRQRPGTDVALLNGLMHIILEKGWENKEFIAQ
                     RTEGFDEFKTLIDAYPPEKVSEITRIPVEKLYQAAEILATNSPMAVMWAMGITQHITG
                     VRNVMDLANLQMLLGNMGIPGGGVNPLRGQNNVQGACDMGGLPNVYPGYQAVTLEAAR
                     EKFAAAWGAPMDDKVGLTVTEMIPQAGEGKIKALYILAEDPVMSDPDTAHIRHCLDTC
                     DFIVLQEIFPSETYPYADVVLPGVSFTEKTGTFTNTERRVQIVRQAIDPVGDARPDWQ
                     ITASLAKRMLALGEPRVQGGTHAGWDYASTSEIMAEINALTPSYAGVTHARLERGDQL
                     QWPVKDSEHPGTPILHTAAFARGLGKFMPIEHVPPAEMPDSEYPVLLSTGRVLYHWHG
                     GSMTRRAEGLLEVYSQALIEVNPLDAQSLGLNGNKRVRVTSRRGSIEAEAWITDRVPP
                     GMVFANFHFPEASANELTLAALDPVAKIPEYKICAVRVEAA"
BASE COUNT          381 a          608 c          665 g          477 t
ORIGIN      
        1 ctcatatact ttgcgtatat ccatattaat ctcctcaggt tgttggtggt tggaaaaccg
       61 gaccaggttg gaaaaccgga cccggtttta gcctggtccg gttttttttg cctggttcag
      121 gcggcttcga cgcgcacagc acagatctta tattccggaa ttttggcaac cggatcgagg
      181 gctgccaggg tcagctcgtt ggccgaagct tccgggaagt ggaaatttgc gaacaccatt
      241 cccgggggga cgcggtccgt gatccaggct tctgcttcga tgctcccgcg gcgggaggta
      301 acccggacgc gtttgttccc attcaggccc aggctctggg cgtccagcgg gttaacttcg
      361 atcaatgcct ggctgtacac ttccagcaag ccttcggcgc ggcgggtcat cgagccgccg
      421 tgccagtgat aaagcacccg cccggtgctc agcaggacag gatactccga gtcgggcatt
      481 tccgcgggtg ggacgtgctc gatgggcatg aatttaccca ggccgcgggc aaatgcggcc
      541 gtatgcagaa tcggtgttcc gggatgttcg ctgtctttaa ccggccactg cagctggtcg
      601 ccgcgctcca ggcgggcatg ggttaccccg gcgtagctgg gagtcagggc gttaatctcg
      661 gccatgatct cagacgtgct ggcataatcc cagccggcgt gggttccacc ctgaactctg
      721 ggctcgccca gggccaacat ccgcttcgcc aggctggctg taatctgcca gtccgggcgg
      781 gcatctccca cgggatcgat cgcctggcgg actatctgga cccgccgttc ggtattggtg
      841 aaagtgccgg tcttttccgt gaagctcacc ccgggcagca cgacatccgc gtagggatag
      901 gtctcggagg ggaaaatctc ctgcaggacg atgaaatcac acgtatccag gcagtggcga
      961 atgtgagcgg tatccgggtc actcatcacc ggatcttcgg ccaggatgta cagggctttg
     1021 atcttgcctt cgccagcctg cgggatcatt tccgttaccg tcaggccgac cttgtcgtcc
     1081 atcggtgctc cccaggcagc tgcgaatttc tcgcgggcag cctccagggt gacggcctgg
     1141 tagccggggt agacattcgg caggccgccc atatcgcagg cgccctggac attattctgg
     1201 ccgcgcagcg ggttcacacc gccgcccggg atgcccatat tgcccagcag catctgtaaa
     1261 ttggccaggt ccatcacgtt gcgcaccccg gtgatgtgtt gagtgatgcc catggcccac
     1321 atgactgcca tcgggctgtt cgtcgccagg atctctgccg cctggtagag tttttccacc
     1381 ggtatccggg tgatctcgga tactttttcg ggcgggtagg cgtcgatcag ggttttgaat
     1441 tcgtcgaagc cttccgtgcg ctgggcgatg aattctttgt tttcccagcc cttttcgaga
     1501 atgatgtgca tcaggccgtt gagcagggcg acatcggtgc cggggcgctg gcggaggtgt
     1561 aaagtggcga actcggtgag atcgatcttg cgtggatccg cgacgaccag cttgacgccg
     1621 cgctgcagaa ctgcctggcg gattttagcg ccaaagaccg gatgctgttc ggtggtattg
     1681 gagccgatca ccagcatcga cttggcaaac tgggcgacat cttccattga gttggacatt
     1741 gcacccgatc caaatgaagt ggccagaccg gccacagtgc tggagtgtca aagccgggcg
     1801 cagtggtcga tggtattggt gccgaccacc tggcgggcaa acttattcat caggtaattt
     1861 tcttcattga gacatttggc ggaagtcagc acgccgacgc tgcgcgatcc gaatgtatcg
     1921 cgatagccgg tcagcttgcg ggcggtgata tccagggcgg tatcccaatc gacggtcacc
     1981 cactcgccgc ggtctttaac cgccgggcga gcgccgtcgc cttccagcag gtatttgcgc
     2041 acttttggct gggtgaggcg atccgggtgg tggacaaaat catagccgta gcggcctttg
     2101 acgcacaggt gcatattatt gaccggcgca t
//
LOCUS       DATMXK010000569         2337 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_109344, whole genome shotgun sequence.
ACCESSION   DATMXK010000569 DATMXK010000000
VERSION     DATMXK010000569.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2337)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2337)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2337
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_109344"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            71..943
                     /gene="lipA"
                     /locus_tag="VN363_10380"
     CDS             71..943
                     /gene="lipA"
                     /locus_tag="VN363_10380"
                     /EC_number="2.8.1.8"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560977.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016992 - lipoate synthase activity
                     [Evidence IEA];
                     GO_function: GO:0051539 - 4 iron, 4 sulfur cluster binding
                     [Evidence IEA];
                     GO_function: GO:1904047 - S-adenosyl-L-methionine binding
                     [Evidence IEA];
                     GO_process: GO:0009107 - lipoate biosynthetic process
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="lipoyl synthase"
                     /protein_id="HWQ84964.1"
                     /translation="MKVRAPAGETYEWLHNLMRSKALHTVCEEAMCPNMGECWGSGTA
                     TFLMLGDVCTRTCGFCDIKRGQPSPLDWMEPERVAQAVKAMNLKHAVITSVNRDDRKD
                     GGAPIFAMVIQRIRQLQPGCSIEVLIPDFKGSLEALKIVMDARPEILNHNVETVPRLF
                     KQVQPQDNYEWAAATLSNAKKLDPEVLCKSGIMLGLGETMDEVKAVMRDQRSWGVDIL
                     TLGQYLQPSKKHLPIERYYTLEEFDELREYGLSIGFRWVESGPLVRSSYHAHEQVRAL
                     SVVHRQLYGASQTN"
     gene            complement(996..2039)
                     /locus_tag="VN363_10385"
     CDS             complement(996..2039)
                     /locus_tag="VN363_10385"
                     /inference="COORDINATES: protein motif:HMM:NF012551.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="endo-1,4-beta-xylanase"
                     /protein_id="HWQ84965.1"
                     /translation="MAEVSAPTSQASPTVKLPPTSTSIPPTLRQLADEHGLLVGAAID
                     PSYLTNSQYTALFEAEFNGLVAENVMKWGRLEQQRGVYDFSKADALMDYAAQQQLAVR
                     GHVLVWDLQLPEWLTQSNLSRDEYKAILKDHITTVVGRYAGRIVAWDVVNEPLDEQGQ
                     IRQNFWFQAIGPEYIPLAFQWAHAADPAAQLFINESYAEGMSEKSQGVFALVSGMLEK
                     GVPIDGVGMQMHLRLDYPPVGEEVAQNMQRLAALGLKVHITELDVRLQDIPGSLEEKL
                     AAQALVAGEMARTCLAAPNCEAFFTWGLTDHYSWIPGTTGKDDQPLLFAENWEPKLAY
                     WAVHAALQDALLK"
BASE COUNT          566 a          640 c          610 g          521 t
ORIGIN      
        1 ctttaatccc actcgcgctc gggtcaatcc cgacgaagtt caaacttccc cacaacgcag
       61 accggattgg atcaaagttc gcgctcctgc tggcgagacc tatgagtggc tgcacaacct
      121 gatgcgcagc aaggcccttc acaccgtttg tgaggaggcc atgtgcccga acatgggtga
      181 gtgttgggga tcaggcacag cgacattctt gatgctgggg gatgtctgta cgcgaacctg
      241 tggcttttgc gatatcaaac gtggccaacc ctccccgcta gattggatgg agccggaacg
      301 cgtcgctcag gctgtcaagg cgatgaattt aaaacacgcc gtcattacca gcgttaaccg
      361 ggatgatcgc aaagacggcg gggcgcctat ttttgcgatg gtgatccagc gcattcgcca
      421 gctccaaccg ggctgttcga tcgaagtact catcccagat ttcaaaggga gcctggaagc
      481 tttgaagatc gtcatggatg cccgcccgga aatccttaac cataatgtcg aaaccgtacc
      541 gcgcctcttc aaacaggtgc agccccagga caattacgag tgggcggcag cgaccttgag
      601 caacgccaaa aagctcgatc ccgaagtgtt gtgcaaatcg gggatcatgc tgggtctggg
      661 tgagacgatg gatgaagtca aagccgtcat gcgcgaccag cgcagctggg gagtcgacat
      721 tctcacgctg gggcagtatc tacagcccag caagaaacat ttacccattg agcgttatta
      781 cactctggaa gaattcgatg aactacgtga atatgggctt tcgatcggct tcagatgggt
      841 ggaaagcggg cctctggtcc gatcttctta ccacgcccac gagcaggtgc gcgcattaag
      901 cgtcgttcac cgccagctgt acggtgccag ccagactaat tgaaattcct gaactgtact
      961 cgaacccggc gaataatccc agatttcgcc gggtattatt taagaagggc atcttgcagg
     1021 gcagcatgga ccgcccaata agccagcttg ggctcccaat tttcagcgaa cagcagcggc
     1081 tggtcatctt taccggtcgt gcctggaatc caggaataat gatcggttaa cccccaggta
     1141 aaaaatgctt cgcagttagg agctgccagg caggtacggg ccatctctcc cgccactaat
     1201 gcctgggcag ccagcttctc ttccaaactt cccgggatat cctgcaagcg cacatccagc
     1261 tcagtaatat gtactttcaa tcccagcgcc gccagccgct gcatattttg ggcaacctcc
     1321 tctcccactg gcggataatc caggcgcagg tgcatttgca tccccacccc atcgatcggg
     1381 acgcctttct cgagcatacc cgagactaag gcaaatactc cctgggattt ttcactcata
     1441 ccttcggcgt aactctcatt aataaataat tgcgccgctg gatcggcagc atgcgcccat
     1501 tgaaatgcca ggggaatgta ttctggaccg atggcctgga accagaaatt ctggcggatt
     1561 tgtccctgct catccagagg ctcattgacc acatcccagg ctacgatccg ccccgcatag
     1621 cgcccaacca ctgtcgttat gtgatcttta aggatggctt tatattcatc gcggctgagg
     1681 ttggattggg tcagccattc gggtaattgc agatcccaca ccaggacatg gccgcgcacc
     1741 gccagctgct gctgggcagc gtaatccatg agcgcatccg ctttagaaaa atcatacaca
     1801 ccgcgttgtt gttccaaccg gccccatttc atcacatttt cagcaaccag accattaaat
     1861 tcagcttcga acaaggcagt atattgcgaa ttggtaagat aggatgggtc gattgctgcc
     1921 ccaaccagca gcccgtgctc atcagccagc tggcgcaagg tgggcggtat ggaagtggag
     1981 gtcggaggca gctttacagt tggagaggcc tggctggttg gagcggagac ctcggccatg
     2041 ttggagtctg gtttacatcc agccaggacc actaacaacg cgaacagact cagaaatccc
     2101 aaaacattaa tctttgagat tgatgaattt gtagcattgc cgcgctttgt catgtgcttc
     2161 agacgctccg tcatcaaagg cagtaaatta cccggggcga ttgcctggat attctaacga
     2221 aaaaaggtgc atcccgacag gatacaccta tagaatttca gctggtgaga cttagaacaa
     2281 tgccaggatt gcctggctgg tccagttaat cagaggtgag gcgataatgc tcaatac
//
LOCUS       DATMXK010000570         2522 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_111952, whole genome shotgun sequence.
ACCESSION   DATMXK010000570 DATMXK010000000
VERSION     DATMXK010000570.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2522)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2522)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2522
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_111952"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..378)
                     /locus_tag="VN363_10390"
     CDS             complement(<1..378)
                     /locus_tag="VN363_10390"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84966.1"
                     /translation="MSRTFLKRKSTRHTCRVDLIDKKTGSAVENQRQVQELIRSRQGR
                     EPIYRTIVCVFGNIVPGRAGQDGIAFGSGILTTRTWRALGCPNIFTGDQTVPAGATLT
                     VEPGVTVTADGDYLIAVYGRLEAV"
     gene            495..1037
                     /locus_tag="VN363_10395"
     CDS             495..1037
                     /locus_tag="VN363_10395"
                     /inference="COORDINATES: protein motif:HMM:NF013936.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="hemerythrin domain-containing protein"
                     /protein_id="HWQ84967.1"
                     /translation="MGKATQDLRAEHEAILNVFRIMDQALVSGAHEADTVLKFGQELV
                     YFLKKFADQCHHGKEENYYFTALAGKGVPEVDGKLTELLAEHGEGRVAIARMNAALAS
                     GDAAGFKNAAIQYRDLLVSHIDKENNDLFVKADALLDEGVQEELAEKFESFEEEVIGH
                     GVHEQLHGMIHQWEQQFIGE"
     gene            complement(1595..2179)
                     /locus_tag="VN363_10400"
     CDS             complement(1595..2179)
                     /locus_tag="VN363_10400"
                     /inference="COORDINATES: protein motif:HMM:NF014689.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="manganese efflux pump MntP family protein"
                     /protein_id="HWQ84968.1"
                     /translation="MEYVTYLLIAVGVAMDVFAVSLGIGCAGQATTARAKFRLAFHFG
                     LFQGGMTLLGYLLGSTVVNLIAGFDHWVAFILLAFVGGRMIKEGLEQLSGEEGYPDER
                     PNGDPSRGGTLVLLSIATSIDALAVGLSMALIQAAIVGASLIIGLTSSGFSLIGLRWG
                     CDLGERFGKRMELLGGLILIGIGVRIVVTHLMAA"
BASE COUNT          621 a          675 c          713 g          513 t
ORIGIN      
        1 aacggcttcc aaccgcccgt acacagcgat cagataatca ccgtcggcag tgaccgttac
       61 ccccggctcc acggtcaggg tcgcccctgc cggaacggtc tggtcgccgg tgaagatatt
      121 ggggcagccc aacgcccgcc aggtgcgcgt ggtcaggatc cccgagccaa aggcgatgcc
      181 atcctggcca gcgcgcccag gtacgatatt cccgaatacg cagacaatgg tacggtatat
      241 gggttcacgc ccctgtcggc tgcggatgag ttcctgcacc tgccgctggt tttcaaccgc
      301 tgacccggtt ttcttatcga tcagatcgac ccggcaggtg tggcgggtcg attttcgttt
      361 aagaaaagtt ctgctcaacc gggcatcaac tgcacttaat tgatccagat catggaacgt
      421 gcagccctaa gcaggtatca tacaggcagc ataacaaaca gaataatgga tacactggaa
      481 agaaggataa acccatggga aaagccacac aagacttacg cgctgaacac gaagcgatcc
      541 tcaatgtctt ccgcatcatg gaccaggcat tggtcagcgg cgcgcacgaa gcagatactg
      601 tactcaaatt tggccaggag ctggtctact tcctgaaaaa gttcgccgac cagtgccacc
      661 acggcaagga agaaaattat tactttacag cgttagccgg caaaggtgtg ccagaagtgg
      721 acgggaagct caccgagctg ctggcggagc acggcgaggg ccgggttgcc atcgcccgga
      781 tgaatgcagc cctcgcatcc ggtgatgcgg cgggcttcaa aaacgcagcc atccagtatc
      841 gcgacctgct ggtcagccac atcgacaagg aaaataacga cctgttcgta aaggcggatg
      901 cgctcctgga tgaaggtgtg caggaagagc tggccgagaa atttgaaagc ttcgaagaag
      961 aggttatcgg gcacggtgtc cacgagcagc tgcatgggat gatccatcaa tgggagcaac
     1021 agtttatcgg ggagtgagcc ggcgcgagga cttataacct ctggaccggt aaacctatga
     1081 aaccccaggg gtctaaatcc tggggatttt tgagacccct ggggtttaaa ggcgtttgga
     1141 accggattac cggcttaggt tgtaataatc gattgatggt tcagggaaaa caatagatgg
     1201 ccgtaaagaa cacggctgga acggattgca cggattgttc gaatctggtc cggtgcgtag
     1261 gggggcagca gtgaatcgaa tgaaattgca cattcccgat cccgctgcgc ccctaatcaa
     1321 tttttacatt cttcttcggt aaccctggtt tggggccgac cgtacaggtt tccgaatgag
     1381 atataggtat ccagactgcc atccagcgca gtgccgccgc ggcttcagat tgccttagaa
     1441 cctgtacggt ctgctcatgg agaatgggtc catcaccgaa ctggtttgtt aaaactgatc
     1501 aggatatcca cttctttatg ctgccatcaa atgggtaact acgctgcgca cgcgcagggc
     1561 ggtgtgcctt tgcgtctatg gcaatttcta ttctttatgc tgccatcaaa tgggtaacta
     1621 cgatgcgcac accaatgccg atcaaaatca gcccgccgag caactccatg cgcttgccga
     1681 agcgctcgcc caggtcgcag ccccagcgca ggccgatcag cgagaatccg ctgctggtga
     1741 gaccgatgat caggctggcg ccgacgatcg ccgcctggat cagggccatg ctcaatccca
     1801 ccgccagggc atcgatgctg gtggcgatgc tcagcagcac cagcgtccca ccccgggatg
     1861 gatcgccgtt ggggcgctcg tccgggtagc cctcctcacc ggatagttgt tcgaggcctt
     1921 ccttgatcat cctcccgccc acaaaagcca gcaggataaa cgccacccag tgatcgaagc
     1981 cggcaatcaa attgaccacc gtgctgccca gcaggtagcc cagcagggtc atgccgccct
     2041 gaaataagcc gaagtggaaa gccaggcgaa atttggcgcg ggcagtggtg gcctgcccgg
     2101 cgcagccgat gcccagcgac acggcgaaca catccatcgc taccccgact gcaatgagca
     2161 gataggttac gtactccatg caggctccag tgggaaaaag gaatgggggg attatatccg
     2221 cgctgggctg gtgcgtgggg agagatttga ttgggaggta tcccagacct gaatgaatta
     2281 ttacattcga tatcgtcaaa ccactcgaac cccaggggac tcaaaacccc aggggtctct
     2341 gaagacccct ggggtttatt gaagatcgaa aattagatga ctgattcagg tgtaaagttc
     2401 gatcaggttg aagattaatt gacatggatg gacaggatat tcaggctgat aatcatagcg
     2461 tgaactggcc tgactggcag gcgcgccaaa ataatcacaa ttattaggct ttggattaga
     2521 at
//
LOCUS       DATMXK010000571         2630 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_2636, whole genome shotgun sequence.
ACCESSION   DATMXK010000571 DATMXK010000000
VERSION     DATMXK010000571.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2630)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2630)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2630
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_2636"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1595
                     /locus_tag="VN363_10405"
     CDS             <1..1595
                     /locus_tag="VN363_10405"
                     /inference="COORDINATES: protein motif:HMM:NF024247.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="4Fe-4S binding protein"
                     /protein_id="HWQ84969.1"
                     /translation="VIAMVGRGTDRRVSTAFGEREAVCDTCNKCVPYCPTGAITHLPD
                     APIGIAFKQDAYKWKRIRQVVQYAFLALFLGLMAFTLLKTSTPSQAPWINLLSRANPL
                     QAIVAMIGGREAIINYWPSLITIALTFVFGRVWCAWICPMGAILELFGRVGRKINMPR
                     LRELKYVILAVIVLMAAFGSLAFMYFEPITIAVRGLTGIFRPVVEYVQIEDKGDFVMP
                     GFAWWAMIIPFALLLVLNVFEKRFWCRYLCPLGAVIGLGSKFAWTKRFVNQESCVQCG
                     ECAGLCTMGAISAKRDYTSDPAECIMCMDCAGPCPKNAISFPKGNFLTFPNEVDPGRR
                     TALGTIGLGALAVGALVMDVGEAKAAKSTVLRPPGAGDDFLAKCIRCDQCIEGCPTHV
                     LQPAAFEGGWDALWTPEFNPFAPAYCDQECNRCGQICPSQAIPNLPVEEKNKQVIGKA
                     FVNFETCVRCMDCLENCPNECFFEVEVEGIRGVFPKVNPENCSGCGKCVWVCPEQEER
                     AIVVYALDSVPEDTYKTTPVTD"
     gene            1654..2067
                     /locus_tag="VN363_10410"
     CDS             1654..2067
                     /locus_tag="VN363_10410"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84970.1"
                     /translation="MIIVWLDLPSVANENAAGRVIVPRGGNMVRVFVLSKNSLFGAGI
                     ESLLGQVAQLDIVTPPEDMASLEERIQECQPDVIIVNCDDPKDELTSAILCALHERLD
                     IRVIGLSLRDNNLFVYRGEQKQIVQVDDLLHAIKD"
     gene            2077..2499
                     /locus_tag="VN363_10415"
     CDS             2077..2499
                     /locus_tag="VN363_10415"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84971.1"
                     /translation="MRLCWLVCLAILLWACTALTQPVISLPTATPAPIPTLADQYAVT
                     AWVNDPEPDPDQNVILSGNLNKNGVILSGIMMEGIWQQPGEEKPSLHCSSLINYQRGR
                     CNIASKDFPLDTYVPLTIHIHYAGMTFIAETGFTPRNK"
BASE COUNT          559 a          721 c          728 g          622 t
ORIGIN      
        1 atgtgattgc tatggtaggg cgcggcactg accgccgggt ttcaacagcc tttggcgagc
       61 gcgaggcagt ctgcgatacc tgtaacaaat gtgtgcctta ctgcccgact ggcgccatta
      121 cccacctgcc cgatgccccc atcgggattg ccttcaagca agatgcctac aagtggaagc
      181 gcatccgtca ggtggtccaa tatgccttcc tggccctgtt cctgggcctg atggcgttca
      241 cactgctcaa aacttccaca ccctctcagg ctccctggat taacctcctc tcccgggcga
      301 acccactcca ggcgattgtc gccatgatcg gtgggcgtga ggcgatcatc aactactggc
      361 cttcgttgat caccattgcc ctgacctttg tctttggccg ggtatggtgc gcctggatct
      421 gcccgatggg tgcgatcctc gagttgtttg gccgggtggg tcgcaagatt aacatgcccc
      481 gcctgcgcga gctcaaatac gtcatcctgg cagtcatcgt gctgatggcg gctttcggaa
      541 gcctggcgtt catgtacttc gagccgatca ccatcgctgt gcgcggcctc accggtatct
      601 tccgcccggt ggtcgagtat gtccagatcg aggacaaggg tgatttcgtc atgcccggct
      661 tcgcctggtg ggcgatgatt atcccctttg ccttactgct ggtcctgaat gtcttcgaaa
      721 agcggttctg gtgccggtac ctgtgcccgc tcggggctgt gattggcctg ggctccaagt
      781 ttgcctggac gaagcgcttt gtgaaccagg aaagctgcgt gcagtgcggt gaatgcgccg
      841 gtctgtgcac catgggcgct atcagcgcca agcgcgatta caccagcgac ccggcggaat
      901 gcattatgtg catggactgc gctgggcctt gccccaagaa cgcgatcagt ttcccgaagg
      961 gcaatttcct gaccttcccg aacgaagtcg atcctgggcg gcggactgcc ctgggtacga
     1021 tcggtctggg cgccctggcg gttggagccc tggtaatgga cgtcggcgaa gccaaagcag
     1081 ccaaatctac cgtcctgcgc ccgcccggag caggggatga tttcctcgcc aagtgtattc
     1141 gctgcgacca gtgtatcgaa ggctgtccca cccatgttct gcaaccggct gcctttgaag
     1201 gtggctggga tgctctgtgg acacccgagt tcaatccctt tgcgccggca tactgcgacc
     1261 aggaatgcaa tcgctgcggg caaatctgcc ccagccaggc tatccccaac ttgcctgtgg
     1321 aagaaaagaa caagcaggtc atcggtaaag ctttcgtgaa ctttgagact tgcgtccgct
     1381 gtatggactg cctggaaaac tgccccaatg aatgcttctt tgaagtagaa gtcgaaggca
     1441 ttcgcggtgt gttccccaaa gttaacccgg agaactgcag cggttgtggt aagtgtgtct
     1501 gggtctgccc tgaacaagaa gaacgggcga tcgtagtata cgcgttagat tcagtgccgg
     1561 aagataccta taaaacaaca cctgtaaccg attagagcgt tacagtctgg cagacccggc
     1621 tcgggggacg gctgggtctg ccagaattac catatgatta tcgtctggct ggacttaccc
     1681 tctgttgcga atgagaacgc ggcaggccga gtgatcgttc ctaggggagg gaatatggtg
     1741 cgcgtttttg tgctttctaa aaattcgctt ttcggggctg ggatcgagag tttactcgga
     1801 caggttgccc agcttgatat tgtcacgcct ccggaagata tggcctctct ggaagaacgg
     1861 atccaggaat gccagccgga tgtgatcatt gtgaactgcg atgatcccaa agatgagctg
     1921 acctcagcga tcctgtgcgc gttacacgag cgcctggata ttcgcgtgat tggtctcagc
     1981 ttgcgggata acaatctctt tgtctatcgc ggcgagcaaa aacaaatcgt ccaggtcgat
     2041 gatttgctgc atgccatcaa ggactagcgc ctacccatgc gtctttgctg gctggtttgc
     2101 ctggcgatcc tgctttgggc ttgcactgca ctgacgcagc cggttatctc cctaccgact
     2161 gctacccctg ctccaatacc cactctggct gatcagtatg cagtaacagc ctgggtgaac
     2221 gaccccgaac cagatcctga tcagaacgtg attttaagcg gcaacttaaa caaaaacggg
     2281 gtgatattaa gtggcatcat gatggaaggg atctggcagc agccagggga agaaaagcca
     2341 tccctgcatt gttcatcttt aataaattat caacgggggc ggtgtaatat cgcttcgaaa
     2401 gattttcccc tggataccta tgtccccctg acaatccata ttcattatgc tggcatgacc
     2461 ttcattgctg agacgggctt tactccccgc aacaagtgat ttcacccgaa atccaatcca
     2521 tcctataaaa aacaataacc agatcttccg aaaactgatc ggataactac gaatgcgctg
     2581 gcacttcacc aatagttgaa gcagcgtaag agtagctacc ggttggatgg
//
LOCUS       DATMXK010000572         5176 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_166999, whole genome shotgun sequence.
ACCESSION   DATMXK010000572 DATMXK010000000
VERSION     DATMXK010000572.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5176)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5176)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5176
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_166999"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            132..905
                     /gene="sufC"
                     /locus_tag="VN363_10420"
     CDS             132..905
                     /gene="sufC"
                     /locus_tag="VN363_10420"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560202.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly ATPase SufC"
                     /protein_id="HWQ84972.1"
                     /translation="MSDLEIRNLHVSIEDKEILKGVNLTIKQGSVHALMGPNGTGKST
                     LAYALMGHPSYTVTEGEAIFEGQNILALQPDVRSHLGIFLAFQYPVAIPGVTVANFLR
                     TAINAKRRAANPEDKGITIPEFRKLLKNKMDLLRMDHNFAGRYLNEGFSGGEKKRAEV
                     LQMAALEPRIAVMDETDSGLDIDALRIVSEGVNALRGPGLGILIITHYQRILNYIKPD
                     YVHIMLDGRIVENGGPELALHLEEQGYDWVREKNEPLAA"
     gene            941..2347
                     /gene="sufB"
                     /locus_tag="VN363_10425"
     CDS             941..2347
                     /gene="sufB"
                     /locus_tag="VN363_10425"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560201.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufB"
                     /protein_id="HWQ84973.1"
                     /translation="MSSDATLLEGLGEYKYGFIDPDVSVFQTRKGLNREVVEQISAKK
                     GEPQWMLDFRLKALEHFQLRPMPMWGGDLSGLNFDEIYFYTKPTNKESGSWDDVPDTI
                     KRTFDRLGIPEAERKFLAGVGAQYESEMVYHSIQESLAAKGVIFKSIENGLRDHPDLF
                     REYFSTVIPIEDNKFAALNSAVWSGGSFVYVPPGVKVDLPMQAYFRMNSADAGQFERT
                     LIIVDEGAQAHYVEGCTAPMYTTDSFHSGVIEIIVKKNARFRYTTIQNWSTNMYNLVT
                     QRAIVHEGGTMEWVDANLGSKLTMKYPSCYLIGERAHGEILSLAFAGNGQHQDTGGKV
                     IHLAPRTTSKITSKSISKGHGRASYRGLLKVHKGAHDSRSNVVCDALLLDNTSRSDTY
                     PSIEIDEDDVTVGHEASVSKVGEEQLFYLMSRGLSQEEATTMVVSGFIEPLVKELPME
                     YAIEMNRLIQLQMEGSIG"
     gene            2432..3796
                     /gene="sufD"
                     /locus_tag="VN363_10430"
     CDS             2432..3796
                     /gene="sufD"
                     /locus_tag="VN363_10430"
                     /inference="COORDINATES: protein motif:HMM:TIGR01981.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005198 - structural molecule activity
                     [Evidence IEA];
                     GO_process: GO:0016226 - iron-sulfur cluster assembly
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Fe-S cluster assembly protein SufD"
                     /protein_id="HWQ84974.1"
                     /translation="MATRTVVSRTRRAEPVETEFNFTREMFTHLSGNGSLPGNLRSYR
                     DQAWDAYSGLSMPTIKDDPWRRTDLKGMPAGSFRLPDAQNTAGLEPAPEDLTVSLAGG
                     THGGRIALGPGGAEAWLDPELAAQGVVFTDLRTAEQKYPDLLGRMLGQSVRTGEGKFA
                     ALAGAGAWTGALVYVPRNVNVEQPLQSLLWGPGAGLAYLSHILVLLEDGASLTYVHEY
                     ASHTETRGQNLHAGIVEIQVGAHANLRFVELQSWGTHFWNFTHERAKVGRDGNLDWIF
                     GSIGSHLTKNFSELELVGEGATGRMSGFYFTDGVQHLDHDTQQNHLAPHTTSDLLFKG
                     ALKDKSRSVWQGMIYVAPGAQKTDGYQANRNLTLSPQARADSIPGLEILADDVRCTHG
                     ATVGKIDPELVFYLRSRGLPYADAERLVVEGFFDPIMQRIPFEGVRERFQKAILEKMG
                     VKAL"
     gene            4026..4334
                     /locus_tag="VN363_10435"
     CDS             4026..4334
                     /locus_tag="VN363_10435"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84975.1"
                     /translation="MPGLHFTQAPESNEPFNVGDRIEVYCDHDREGERIRDWLEGTVV
                     QVDPKMLAIQFQESVYLTDGWMVPDHVLWCPKDSQNIRPVSRRRKKPRVRPTRMDFGG
                     "
     gene            4359..>5176
                     /locus_tag="VN363_10440"
     CDS             4359..>5176
                     /locus_tag="VN363_10440"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560198.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="aminotransferase class V-fold PLP-dependent
                     enzyme"
                     /protein_id="HWQ84976.1"
                     /translation="MSQITTLTDLFDVERIRQDFPILNREVHPGVPLVYLDSTATSQK
                     PLQVIEAMDRFYRQTNANIHRGIHVLAEESTAMYEETRKKVARFINAPSARQIIYTRN
                     TTEAINLVAYSWGRANLNPGDVVILTEMEHHSNLVPWHMLSAEKNLRLEFIPVGDDGL
                     LDLAEYQRLLELEPKLVSFTGMSNVLGTITPAQEIIRLAHAAGAIALMDGAQSVPHYS
                     VDVQALDVDFLAFSGHKMVGPTGIGILYGRKELLNSMPPFMGGGDMIKRVELRS"
BASE COUNT         1175 a         1463 c         1543 g          995 t
ORIGIN      
        1 atattgacgg ataagccatt ttttgctata ataccgcacg ccgggaaaac caagaccggc
       61 tgcagcctgt aacttcccgc ccacgagagc ggtaaaacgc aaaccttact tcatcaagga
      121 gttcgtagtc catgtccgat ctggaaattc gcaacctgca tgtcagcatt gaagataaag
      181 agatcctgaa aggggtcaat ctgaccatca agcaaggctc ggtgcacgcc ctgatggggc
      241 cgaacggcac cggcaaatcc accctggcgt atgccctgat gggccacccc agctataccg
      301 tgacggaggg tgaagcgatc ttcgaaggcc agaacatcct ggcgttgcag ccggatgtgc
      361 gctcgcacct gggtattttt ctggctttcc agtacccggt agcgatcccc ggggtaacgg
      421 tggcgaattt tctgcgcacg gcaatcaacg ccaagcggcg agcagccaac ccggaagata
      481 agggcattac catccctgaa ttccgcaagc tgctcaaaaa taagatggac ctgttgagaa
      541 tggaccacaa tttcgccgga cgttatctga atgaaggctt ttccggcggt gagaagaagc
      601 gcgccgaagt gctgcagatg gcagccctgg agccgcggat agccgtaatg gacgagactg
      661 actccggcct ggatattgat gccctgcgta tcgtatctga aggtgtgaat gccctgcgcg
      721 ggccgggcct gggtatcctg atcatcaccc attaccagcg catcctgaat tacatcaagc
      781 cggattacgt gcatattatg ctcgatggcc ggatcgtcga gaacggtggg ccggagctgg
      841 cattgcacct ggaagagcaa ggctacgatt gggtgcgcga gaaaaacgaa ccgctggcgg
      901 cttaacggta gcgacttaga cggtagattg gagacgaatc atgagctctg acgcaaccct
      961 cctggaaggt ctgggcgaat acaagtacgg ctttatcgac cccgacgttt ccgttttcca
     1021 gacccggaag ggtctgaacc gagaagtggt cgaacaaatc tcggctaaaa aaggcgagcc
     1081 ccaatggatg ctcgatttcc gcctcaaagc gctggagcat ttccagctgc gcccaatgcc
     1141 gatgtggggc ggtgatttga gcgggctcaa ctttgacgaa atttattttt acaccaaacc
     1201 gaccaataaa gagagcggtt cctgggatga tgtgccggat accataaaac gcacgttcga
     1261 ccgcctgggg atccccgaag ccgagcgcaa gttcctggct ggcgtcggcg cgcagtacga
     1321 atcggagatg gtgtaccaca gcatccagga gagcctggcc gcgaaaggtg tgatcttcaa
     1381 gagcatcgag aacggcctgc gcgatcaccc ggatctgttc cgcgaatatt tcagcacggt
     1441 tatcccgatc gaagacaata aatttgcagc cctgaacagc gcggtgtggt cgggcggctc
     1501 gtttgtgtac gtgccgccag gagtgaaggt agacctgccc atgcaggcat acttccgcat
     1561 gaactccgcc gacgccgggc agtttgagcg caccttgatt attgtcgacg aaggggcgca
     1621 ggcccactac gtggaaggct gtaccgcccc catgtacacc accgattcgt tccacagcgg
     1681 cgtgatcgag atcatcgtca agaaaaacgc ccgcttccgc tataccacga tccagaactg
     1741 gtccaccaat atgtacaacc tggtgacaca gcgcgcgatc gtccacgaag gcggtacgat
     1801 ggagtgggtg gacgccaacc tgggctccaa gctgaccatg aagtacccgt cctgctacct
     1861 gatcggcgag cgagcccacg gcgagatcct ttcgctggcc ttcgccggca acgggcagca
     1921 ccaggatacc ggcggcaagg tgatccacct ggcgccgcgc actaccagca agatcacctc
     1981 caagtcgatc agcaaggggc acgggcgggc cagctatcgc ggcctgctca aggtgcacaa
     2041 aggcgcccat gattcccgct cgaatgttgt ttgcgatgca ctgctgctgg acaatacttc
     2101 ccgctcggat acgtacccgt cgattgagat cgatgaagac gatgtgaccg tgggtcacga
     2161 ggcttcggtc tcgaaagtgg gcgaggagca gctcttctac ctgatgagcc gcggcctgag
     2221 ccaggaagag gcgactacca tggtggtttc gggctttatc gagccgctgg tgaaggagct
     2281 gccgatggaa tatgccatcg aaatgaaccg cctgatccag ctgcaaatgg aaggctccat
     2341 cggttaacct gcgctctgcg catgtcgacc agcccaaacc gctggtatgc atgcaggcga
     2401 aaaaacggat ttaggaaaat atacagacac tatggcaacc agaactgtcg tatcacgcac
     2461 tcgccgggct gagccggtag aaaccgaatt caactttact cgcgaaatgt tcactcacct
     2521 gagtgggaac ggctcgctgc cggggaacct gcgcagctac cgcgaccagg cctgggatgc
     2581 atattccggc ctgagcatgc ctacaatcaa agatgatccc tggcggcgga ccgacctgaa
     2641 agggatgccg gcgggaagct tccggctgcc ggatgcccag aacaccgccg ggctggagcc
     2701 ggctcccgag gatcttacgg tctctctggc aggtggaacc catggcggga gaatcgcttt
     2761 aggtcccggc ggggctgaag cctggctcga tccagagctg gccgcccagg gtgtcgtttt
     2821 cacggatcta cggactgccg agcagaagta cccggacctg ctgggacgca tgcttgggca
     2881 gtcggtgcgc accggtgagg gcaagttcgc cgccctggct ggcgcaggcg cctggaccgg
     2941 agcgctggta tacgtgcccc gcaacgtgaa tgttgaacag ccgctgcaaa gcctgctgtg
     3001 ggggccaggc gccgggctgg cgtacctttc gcatatcctg gtgctgctgg aagatggtgc
     3061 atccctgacg tatgtgcacg aatatgcttc gcatactgag acacgtggac agaacctgca
     3121 tgccgggatt gtggagatcc aggtaggggc ccacgccaat ctgcgctttg tagagctgca
     3181 gtcctggggc acgcattttt ggaacttcac ccatgagcgg gctaaagtcg gccgtgacgg
     3241 caacctggac tggatatttg gctcgatcgg ctcgcacctg accaagaatt tctccgagct
     3301 ggagctggtc ggcgaaggcg cgaccgggcg catgtcgggg ttctacttca ccgatggagt
     3361 ccagcacctg gaccacgata cccagcagaa tcatctggcg ccgcatacca cgagcgacct
     3421 gctgttcaaa ggcgcgctga aggataagag ccgctcggtc tggcagggga tgatctacgt
     3481 agcccccgga gcccagaaaa cggatggtta ccaggcaaac cgcaacctga ccctcagccc
     3541 gcaggctcgc gccgactcga tccccggcct tgagatcctg gcggatgatg tgcgctgcac
     3601 gcacggggcg acggtcggca aaatcgaccc cgagctggta ttttacctgc gcagccgcgg
     3661 gctgccgtat gccgatgccg agcgcctggt ggtagaaggc tttttcgatc ccatcatgca
     3721 gcgcatcccg ttcgaaggtg tgcgcgagcg cttccagaag gcgattctgg agaagatggg
     3781 cgtcaaggcg ctgtaataag taagagattt tgttattgcg agcggagcga agcaatccgg
     3841 cgctggaatg atttgaaatt tgccagcact gagattgctt cggtcgcaca gatcgctccc
     3901 tcgcaatgac agttgaaaaa ctaaaaaaaa ttaaccagcg aaacgatcga cgacgcaaat
     3961 caaacaaaaa acgtttgatt tgctccttga aagcctggct ggagtcagct cacaaggagg
     4021 ccgagttgcc aggactgcac tttacccaag cgcctgaatc caacgagccg ttcaatgttg
     4081 gcgaccggat cgaggtgtac tgcgaccacg accgggaagg cgaacgcatc cgcgattggc
     4141 tggaaggcac ggtggtccag gtggacccta aaatgcttgc tatccagttc caggagagcg
     4201 tctacctgac cgacggatgg atggtgcccg atcacgttct gtggtgtccc aaggattctc
     4261 aaaatatccg cccggtttcc cgccggcgga agaaaccccg cgtccgtccg acccggatgg
     4321 attttggcgg gtagcccaga ttaatcaaaa gagtgccaat gtcacagata accacactca
     4381 ctgacctgtt tgatgtcgag cggatccggc aggacttccc gattctgaac cgggaagtcc
     4441 atcccggcgt tccgctggtt tatctggatt ccacagctac cagccagaaa ccactccagg
     4501 tgatcgaggc gatggaccgg ttttaccgcc agaccaacgc caatattcac cgcggcatcc
     4561 atgtcctggc agaagaatcg actgccatgt atgaagagac gcgcaaaaaa gtggcccgct
     4621 ttatcaacgc gccatctgcc cggcagatta tctatacccg caataccacc gaggcgatca
     4681 acctggtggc atacagctgg gggcgggcga acctgaaccc tggcgatgtg gtgatcctga
     4741 ccgagatgga acaccactcg aacctggtgc cctggcatat gctcagcgct gagaagaatc
     4801 tgcgcctgga gttcattccg gtgggcgatg atgggctgct cgacctggcg gaataccagc
     4861 gcctgctgga gctagagccc aagctggtca gctttacggg gatgtccaac gtactgggga
     4921 cgatcacccc ggcacaggag atcatccggc tggctcacgc ggccggggca atcgcattaa
     4981 tggatggcgc ccaatcggtg ccgcactaca gtgtggatgt gcaggctctg gatgtcgatt
     5041 tcctggcttt ttccggccac aaaatggtcg gcccgaccgg gatcggcatt ctgtacgggc
     5101 gcaaagagct gctgaacagt atgccgccct tcatgggcgg tggggatatg atcaagcggg
     5161 tcgagctgcg cagctt
//
LOCUS       DATMXK010000573         5447 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_168645, whole genome shotgun sequence.
ACCESSION   DATMXK010000573 DATMXK010000000
VERSION     DATMXK010000573.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5447)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5447)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5447
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168645"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..2339
                     /locus_tag="VN363_10445"
     CDS             <1..2339
                     /locus_tag="VN363_10445"
                     /inference="COORDINATES: protein motif:HMM:NF037144.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="Ig-like domain-containing protein"
                     /protein_id="HWQ84977.1"
                     /translation="IFPGENLVHIPDTTAPGNWDIIGSLAGTQFFAGDFTAGDFSTLY
                     VADYGNNNLYAVDTATAAVTLIGPTTPPGGHTFTGLTGEPDGQLYGLTSSCGGSSLVS
                     VDPATGATTELGSLPGIDCGIDLAYNTNEDMLYIVDIITDSLFRVDPASLDITLVGSL
                     GFDANFAQGLDFEEETGILYWAAYGFAGELRVIDMTTGASALIGGFPGGAEVDSLAFT
                     TGGSSDVPWFSEAPVSGVIAGGSSQDITVTFDPAPLPQPGDYLGEIRVGHDTPYTYPD
                     IALTLHLQAPASWGTFNGTVNGLERCDVNPAALEGSTVNIYDSDGVLLTSLETNELGY
                     YSWSLLNGTYDLEVIAAGYVSQTIEDVVLGAGATVITNFNLRLNAPCLGVDPGALEQT
                     MSPDRTRSQVLTINNTGAGAGSFNLLEMDPLEAAVNADVELILDDGSAEDSIGLTAGG
                     QFLWFNRFTPNPTALPFFLDEVLTLFNNTVQVGDEMQLVVYSDADGDPSNGATYLGGE
                     SFTVVTNDLATWNSFTLADPIMIQDPGDVLIGFVNRSGAAGTSDFPAAIDMDSSQQRS
                     WVALYSGDPPAEPPLPSDGEMSLIDDAGLPGNWNIRGMGTSATADILWLEEAPVSGSV
                     AGDSSSDVTITFDSAGLDLGDYFANLRVRNAAYPPTDVPVTLHVIENALPIALDDAYS
                     MLEEGVLSVDAAAGPLANDTDPLGDPLTVELVDDVSHGTLVLLANGFFVYVPVFDFTG
                     VDTFTYRAFDGEAYSLPATVSITVNEVVANLFLPGIWR"
     gene            complement(2416..3606)
                     /locus_tag="VN363_10450"
     CDS             complement(2416..3606)
                     /locus_tag="VN363_10450"
                     /inference="COORDINATES: protein motif:HMM:NF024110.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0140359 - ABC-type transporter activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ABC transporter permease"
                     /protein_id="HWQ84978.1"
                     /translation="MNAFSRVWIVFYKEIVDNLRDRRSVLSALLSTLIGPAILLLLIV
                     ILGKSVFNEDSSEPLEISLAGGEHAPGLVAFLEQNGAILKPAPADPAAAVRNGDLEVV
                     MIIPTGYGEDFTSGKPATVELVQDSSRQSAMSNVQRARSLLSAYSQQTGAQRLLARGI
                     SPTITNALSLEVVDVSTPESQSLIFLNMMPYFIVMVVFTGGMYVIIDATAGERERGSL
                     EPLLINPIGRREIVIGKLLASLPFAALAVAFTLIAFGLGFNVFPIEDYLGFQLALDVS
                     ALFGIFLISLPMVLLASAVQTVIVTFTRSFKEAQTYVGFLPLIPALPGLALAFLPVKP
                     ELWMMLIPTFGQQILINQLMRGEVINSLFVVLSTAATLLLALALVWVAIRLYSRERIL
                     FGAR"
     gene            complement(3603..4340)
                     /locus_tag="VN363_10455"
     CDS             complement(3603..4340)
                     /locus_tag="VN363_10455"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560953.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding cassette domain-containing protein"
                     /protein_id="HWQ84979.1"
                     /translation="MIQVTDLHKSFNEVKALQGVSFSAPDGQITGLLGPNGAGKTTTL
                     RVLYTIIRQDRGTATVDGFDTLTQPLQVQRSIGALPDSQGLYPRLTARENIRYYGRLH
                     NLGGAELESRIDHLAGMLEMDDFLERRTEGFSTGQRVKVAIARALVHRPGNVLLDEPT
                     SGLDVMSTRSMRTFIRRIKDEGQCVLFSSHIMQEVSALCDNIIILQGGRVVASGTPAE
                     LLRQTGQANLEDAFVAAIGTEEGLVRA"
     gene            complement(4356..>5447)
                     /locus_tag="VN363_10460"
     CDS             complement(4356..>5447)
                     /locus_tag="VN363_10460"
                     /inference="COORDINATES: protein motif:HMM:NF019982.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016787 - hydrolase activity [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="alpha/beta hydrolase"
                     /protein_id="HWQ84980.1"
                     /translation="PLDCPDSPDATGDPAADQAALETWVSACLDGLDANPALYTTSIA
                     MQDLDDVRQALGYERINLYGLSYGTRAALTYLQMFPDNVRTLILDGVVPQDEALGLDM
                     ASDAQRALDLLFERCAADAACSEAFPDLDASFKQLLAQLEAAPVEVTFLHPVNGGQET
                     HPFTAVELVSTVRLLSYTPETAALLPLLIHSAAKENRLDLLTAQSRLVAEELSQSISM
                     GMNLSVLCAEDFPFIDAAAAELASQNTYLGNLQYEELAALCAIWPQGETPAGFKDRVS
                     ADAPVLLLSGEADPVTPPENAEQVAATLPNSLVLTAAGQGHNVIFRGCMPQIAYDFIQ
                     SGSLRGLDTDCVSQIKPSPFFLTFSGPLP"
BASE COUNT         1141 a         1595 c         1653 g         1058 t
ORIGIN      
        1 atatcttccc gggtgaaaac ctggttcata tccccgatac caccgctccc ggcaactggg
       61 atattatcgg gagtcttgcg ggtacccagt tcttcgccgg cgacttcaca gccggtgact
      121 tcagcacact gtatgtggct gactacggga acaacaacct ctacgccgtc gataccgcga
      181 ctgcggcggt cacactgatc gggccaacca cccctcccgg agggcatacc ttcacgggtc
      241 tcaccggcga gccggacggc cagctgtatg gcttaaccag ctcgtgcggt ggaagctctc
      301 tggtaagcgt tgatccggct acgggagcaa ccactgagct cggaagcctg cccgggatcg
      361 actgcggcat cgatctggca tacaacacca acgaagacat gctctacatc gtcgatatca
      421 tcaccgacag cctgttccgg gtggatccag ccagcctgga tatcaccctg gtcggctcgt
      481 taggtttcga cgctaacttt gcccagggcc tggacttcga agaagagacc ggcatcctat
      541 actgggcagc ctacggcttc gcaggtgagc tgcgggtgat cgatatgacc accggcgcca
      601 gcgcgctgat cggcggcttc cctggcggcg ctgaagtcga cagcctggca ttcaccaccg
      661 gcggcagctc cgatgtgccc tggttcagtg aagctcctgt cagcggggta atcgccggcg
      721 gcagctcgca ggatataacg gttactttcg atcctgcccc gctaccccag ccaggcgatt
      781 acctgggtga gatccgggtt ggtcatgaca caccctacac ttatccagac attgccctca
      841 ccctgcacct gcaggccccg gccagttggg gcactttcaa tgggactgtc aatggtctcg
      901 aacgctgcga tgtgaatcca gctgcgctgg aagggtcgac ggtcaatatc tatgacagcg
      961 acggcgttct gttaaccagc ctcgaaacca acgagctggg ctactacagc tggtcgctgc
     1021 tcaatggcac ttacgatctc gaagtgatcg ctgccggata tgtctctcag acaatcgagg
     1081 atgtggtcct gggtgccggt gcgactgtca tcaccaactt caacctgcgc ctgaatgcac
     1141 cctgtctcgg cgtcgatccg ggcgcactgg aacagacgat gtctccagac cggactcgga
     1201 gccaggtgct cacgatcaac aacaccggcg ccggagcagg cagcttcaac ctgctcgaga
     1261 tggatccgct ggaggctgcg gtcaacgctg acgtagaatt gatcctggat gacggcagcg
     1321 ccgaagattc cattggtctg accgccggag gtcaattcct gtggttcaac cgctttacac
     1381 ccaatccgac tgccctgccg ttcttcctcg acgaggtgtt gaccctgttc aacaacacgg
     1441 tccaggtcgg cgacgaaatg cagctggtgg tgtacagtga tgccgacggc gatccgagca
     1501 atggggcgac ctacctgggt ggcgaatcct ttacagtggt caccaacgac ctggcgacct
     1561 ggaacagctt cacccttgct gacccgatca tgatccagga cccgggggac gtgctgatcg
     1621 gtttcgtcaa tcgctctggg gcagccggga cttccgattt cccggcggcg atcgatatgg
     1681 atagttcgca gcagcgctcc tgggtagctc tctacagcgg cgatccgccg gcagagccgc
     1741 cgctgccatc cgacggcgag atgtcgctga tcgatgatgc cggtctaccc ggtaactgga
     1801 atatccgcgg catgggtacc tcggcgaccg cagatatcct gtggctggaa gaagcaccgg
     1861 tatcaggcag cgtggccggc gactccagca gcgacgtcac catcactttc gactcagcag
     1921 ggttggatct gggcgactac tttgccaacc tgagagtgcg caatgcggcc tatccgccaa
     1981 ccgatgtacc ggtgacactg catgtcattg agaatgctct tccgatcgcc ctggatgacg
     2041 cctactctat gcttgaggaa ggcgtactct cggtggatgc ggcggcagga ccgctggcga
     2101 acgatacaga tcccctgggc gacccgctga cggtcgagct ggtggacgat gtgtcccacg
     2161 gtacgctggt gctgctggcg aacggcttct tcgtctacgt acccgtgttc gacttcaccg
     2221 gtgtggatac cttcacctac cgcgcgtttg acggtgaggc ttactcacta ccagcgactg
     2281 tttcgatcac cgtaaacgag gtggtcgcca atctcttctt accggggatc tggcgctaaa
     2341 ccgctaatct ccccctcgct ataacccacc ggggttgtcc aggcagctgg gcgaccccgg
     2401 tggctttaag tatgcctaac gcgctccaaa caggattcgc tcgcggctgt acaggcgaat
     2461 cgccacccag accagcgcca gggcgaggag cagcgtggca gccgtcgaga ggacaacaaa
     2521 cagggagttg atcacttcgc cgcgcatgag ctggttgatc aatatttgct gtccaaaggt
     2581 ggggatcagc atcatccaga gctcaggctt gaccggcaag aatgccagag ccaggccggg
     2641 taaagccggg atgagcggca gaaagccgac ataagtctgg gcttctttga aactgcgggt
     2701 gaaggtgacg attacagtct ggactgccga agccagcagc accatcggca gggagatcag
     2761 gaagatgcca aacagggcac tgacatcgag agcaagctgg aagcccaggt aatcttcgat
     2821 cggaaagacg ttgaaaccca ggccaaaagc gatcaacgtg aaagccacag ccagggctgc
     2881 gaaaggcagg gaagccagca gtttgccgat gacaatctca cggcgcccga tggggttaat
     2941 cagcagtggc tccaacgaac cacgctcgcg ctcgccggca gtggcgtcga tgatcacata
     3001 cataccgccg gtgaagacca ccatgacgat gaaatagggc atcatattga gaaaaatcag
     3061 ggattggctc tcaggtgtgg aaacatcgac tacttcgagc gagagggcat tagtgattgt
     3121 ggggctgata ccgcgcgcca gcaggcgctg cgctccggtt tgctggctat aggcagagag
     3181 caggctgcgg gcgcgctgga cgttagacat tgctgattga cgagagctgt cctggaccag
     3241 ctctaccgtc gccggttttc cggaggtgaa atcctctccg tagccggtag ggatgatcat
     3301 gaccacttcc aggtcaccgt tgcggactgc cgcggcaggg tcagcgggtg ccggcttgag
     3361 gatggctcca ttttgctcga ggaacgcaac caggccgggg gcgtgttccc cgcctgccag
     3421 ggagatttcc aggggttcgc tgctgtcctc attgaagact gatttgccca gaatgacaat
     3481 caggagcagc aaaatcgcag gaccgatcag ggtgctcagc agggcgctga gcaccgagcg
     3541 ccggtcgcgc agattatcta caatttcctt gtagaaaacg atccagactc gggagaaagc
     3601 attcatgctc gcaccagccc ttcctcggtt ccgattgcag ctacaaaagc gtcttccagg
     3661 ttggcctgcc cggtctggcg cagcagctcg gcaggcgtgc cgctggcgac gactcttccg
     3721 ccctgcagga tgatgatgtt atcgcacagg gcagatactt cctgcatgat gtggctggag
     3781 aacaacacac actggccttc gtctttgatg cggcggataa atgtgcgcat agagcgggtg
     3841 ctcatcacat ccagcccgga ggtaggctca tccagaagca cattgcccgg cctatggacc
     3901 agggcacggg cgatagcgac tttcacgcgc tgcccggtgg aaaaaccttc ggtgcgccgc
     3961 tcgaggaaat catccatttc cagcataccc gccaggtggt cgatgcggct ctccaactca
     4021 gccccgccca gattgtgcag ccgcccgtaa tagcggatat tctcgcgggc agtcaggcgc
     4081 gggtaaaggc cctgactatc gggcagggcg ccgatactgc gctgcacctg caagggctgg
     4141 gtgagggtgt cgaatccgtc cacagtagct gtgccgcgat cctggcggat gatcgtgtac
     4201 agcacacgca gggtggtggt cttgccggct ccgtttgggc ccagcagccc ggtgatctgc
     4261 ccgtcggggg ctgaaaaact caccccctgt aaggctttga cttcattaaa tgatttatgg
     4321 agatcggtta cctggatcat ggcagggtcc tcggcttacg gcagcgggcc gctgaaggtc
     4381 aggaagaacg gggaaggttt gatctgtgaa acacagtcag tatccagacc ccggaggctt
     4441 ccagattgga tgaaatcata ggcgatctga ggcatacacc cccgaaagat cacattatgg
     4501 ccctggccgg cagcagtcag caccaggctg ttgggcaggg tcgcagcgac ctgttcggcg
     4561 ttctcaggag gcgtaaccgg gtctgcctcg ccggatagga gcaggaccgg agcatcggcg
     4621 ctcacccgat ccttgaaacc ggcaggtgtt tcaccctggg gccagatggc gcacagcgcc
     4681 gccagctcct cgtattggag attccccagg taggtattct gggaggcaag ctcagcggca
     4741 gcggcatcta taaaagggaa atcttctgcg cacagcacgc tcaggttcat ccccatactg
     4801 atcgattggc tgagttcctc ggcaaccagc cgggattgag cagtcagcag gtcaagccgg
     4861 ttttctttgg cggcagaatg gatcaacagg ggcagcaagg cagctgtctc aggggtatag
     4921 ctcaacaggc gcaccgtgga gaccagctca actgcggtga acggatgggt ttcctgccca
     4981 ccattcaccg gatggaggaa agtaacctcg accggagctg cctccaactg cgccaataac
     5041 tgcttaaaac tggcatccag atcggggaaa gcctcactac aggccgcatc agctgcgcag
     5101 cgttcgaaga gcaggtcgag ggcacgctgg gcatcggagg ccatatccag accaagggct
     5161 tcatcttgcg gcacaacccc atccaggatc agggtgcgca cattgtccgg gaacatctgg
     5221 aggtaggtca gggcggcccg cgtgccgtac gacaaaccgt acaggtttat gcgctcgtag
     5281 cccagggcct ggcggacatc gtccagatct tgcatggcta tggatgtggt atagagggcc
     5341 gggttggcat ccaggccatc caggcaggcg ctcacccagg tttccagggc cgcctggtca
     5401 gcggctggat ctccggttgc atcggggcta tcggggcagt cgagcgg
//
LOCUS       DATMXK010000574         2124 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_42438, whole genome shotgun sequence.
ACCESSION   DATMXK010000574 DATMXK010000000
VERSION     DATMXK010000574.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2124)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2124)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2124
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42438"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..643
                     /locus_tag="VN363_10465"
     CDS             <1..643
                     /locus_tag="VN363_10465"
                     /inference="COORDINATES: protein motif:HMM:NF019847.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008168 - methyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0008757 - S-adenosylmethionine-dependent
                     methyltransferase activity [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="methyltransferase domain-containing protein"
                     /protein_id="HWQ84981.1"
                     /translation="AIWEHAAPATITACDPSPGFIEFARRSLSHPEINFEVAGTAELP
                     RHAGGFDAAAAGLVLNFLPDPGAAVAAMGERLKPGGWLAGYVWDYAEGMQFIKRFWEA
                     AAEMDPRAAELDEGPRFPLCHPPALAALLEEAGLERVEVGALEIETHFADFEDYWRPF
                     LGGTGPGPAYVAALEADAREALRLRLRQELSPNADGPVRLSARAWAVRGARGS"
     gene            complement(789..>2124)
                     /locus_tag="VN363_10470"
     CDS             complement(789..>2124)
                     /locus_tag="VN363_10470"
                     /inference="COORDINATES: protein motif:HMM:NF019365.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="arabinogalactan endo-1,4-beta-galactosidase"
                     /protein_id="HWQ84982.1"
                     /translation="PYVVETSQTLTGIPRGWYTLKAWVKSSGGQNAAYIALKNCANNS
                     DEIFAAHLPVAPPDRWVQVVVSAFVKHGQCTISLYSDANAGNWANFDDIEFVPGQASL
                     SILGADISSLKKSEDYGGIYRTAAGKPRDALQILRSEGMNWARLRAWVDPADGYHDTE
                     ELLEMARRIRERQMQLLVDLHYSDTWADPGKQYKPAAWEGLTTDELVQTVYDYTFDIC
                     QRLVAQDTPPAMVQIGNELNSGMLWPDGHTWNPPNWDNLARLLTAGYNAVKDCSPETR
                     VMLHLANGGDNGLYRWWFGEITARSVPFDVIGFSYYSFWHGSLADLQYNLNDISARYD
                     KDVVVVETAYPFTLADADGWENIIDLESELTNGYPATPAGQAAMLRDVMAIVRAVPDG
                     RGLGVFYWDATWTGVPGNGWDPADPASGNAWENQALFDFDDISLPALDEFKP"
BASE COUNT          386 a          615 c          741 g          382 t
ORIGIN      
        1 ggccatctgg gagcatgcgg caccagccac catcacagcc tgcgaccctt cgccgggatt
       61 cattgaattc gcccggcgga gcctctccca tccggagatc aacttcgagg tggcgggtac
      121 ggctgagctg ccgcgccatg cgggtggatt cgacgcggcg gcggccggcc tggtgctgaa
      181 cttcctgccc gatccgggcg cggcggtggc ggcgatgggc gagcggctga aaccgggggg
      241 ctggctggct ggctacgtgt gggattatgc ggaggggatg cagtttatca aacgcttctg
      301 ggaggcggca gccgaaatgg acccccgggc ggctgagctg gacgaagggc cgcgctttcc
      361 actgtgccac ccgccggcgc tggcagcgct gctggaggaa gccggccttg agcgggtgga
      421 ggtaggagcc ctggaaattg agacccattt cgcagatttc gaggattact ggcggccatt
      481 tctgggcggg accggtcccg gacctgccta tgtggctgcg cttgaagcgg acgcccggga
      541 agcgctgcgg ctgcggctgc ggcaggagct cagcccaaat gcggatgggc cggtccggct
      601 ttcggcccgc gcctgggcgg tgcgcggggc gcgggggagc tgaaaacctg gatcggcgcg
      661 gaaaagccgg gttaacgaat tttcaaccct cacccggcgc agaccaagcg taagcaaagg
      721 gagatcggca gagaacgccg atctcctttg tcagatcaag ctcgatagat cactgcgagc
      781 gctctcgact acggcttgaa ctcgtccagg gcgggcaggc taatgtcgtc aaaatcgaag
      841 agagcctgat tctcccaggc gttgcccgaa gccggatcag ccggatccca gccattgccg
      901 ggaacaccgg tccaggtggc atcccagtag aagacgccca gaccgcggcc atcgggtacg
      961 gcgcgcacaa tggccatcac gtcccggagc atggcagcct gtccggcagg cgtggcgggg
     1021 tagccgttgg tcagctcgct ctccaggtcg atgatatttt cccagccgtc ggcgtccgcc
     1081 agggtgaagg ggtaggcggt ttcaaccacg accacgtctt tatcataccg ggcgctgata
     1141 tcgttgaggt tgtactgcag atcggccagg gagccgtgcc agaaggagta gtacgagaag
     1201 ccgatgacat cgaaaggcac gctgcgggcg gtgatctcac caaaccacca gcggtacagt
     1261 ccattgtcgc cgccgttcgc caggtggagc atgaccctgg tttcggggga gcagtccttc
     1321 acggcattgt agccggcagt cagcaggcgg gccaggttgt cccaattggg cgggttccag
     1381 gtgtgcccgt cgggccagag cataccggaa ttcagctcat tgccgatctg gaccatggca
     1441 ggcggggtgt cctgggctac caggcgctgg cagatatcga aggtgtaatc gtagacggtc
     1501 tgcaccagct catcagtggt aaggccctcc caggcggccg gcttgtactg cttgccgggg
     1561 tcggcccagg tatcggagta atgcaggtcc acgagcagct gcatctgcct ttcccgaata
     1621 cgccgggcca tctccaggag ctcctcggta tcgtgataac cgtcggcagg atcgacccag
     1681 gcgcgcaggc gagcccagtt catgccctcg ctgcgcagga tctggagggc atcgcgcggc
     1741 ttgccagcgg cggtgcggta aattccgccg taatcttcgg atttcttgag actggaaata
     1801 tctgcgccga ggatggacag gctggcctgg ccggggacga attcgatgtc gtcgaagttg
     1861 gcccagttgc cggcattggc atcggagtaa aggctgatgg tgcactgccc atgcttgacg
     1921 aaggccgaga cgaccacctg cacccagcga tccggcgggg cgaccggcag atgggcagcg
     1981 aagatctcgt cagaattgtt ggcgcagttc ttgagggcga tgtaggctgc attctgcccg
     2041 ccgctggact tgacccaggc tttcagggtg taccagccgc ggggtatgcc ggtgagggtc
     2101 tgggaagttt caacgacata gggg
//
LOCUS       DATMXK010000575         3379 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_30249, whole genome shotgun sequence.
ACCESSION   DATMXK010000575 DATMXK010000000
VERSION     DATMXK010000575.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3379)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3379)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3379
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_30249"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..404
                     /locus_tag="VN363_10475"
     CDS             <1..404
                     /locus_tag="VN363_10475"
                     /inference="COORDINATES: protein motif:HMM:NF024591.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84983.1"
                     /translation="YILGRSDDTLKIAGKRLGPAEVESILVNHPSVIEAAAIGIPDEI
                     KGNALIVYCVLAADCGACEELRHELAELLVHHLGKALAPKAIYFVADLPKTRNAKVMR
                     RMIRAAYLGQDPGDTSSLVNPEAVEAIRNSL"
     gene            487..1638
                     /locus_tag="VN363_10480"
     CDS             487..1638
                     /locus_tag="VN363_10480"
                     /inference="COORDINATES: protein motif:HMM:NF012656.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0016627 - oxidoreductase activity, acting
                     on the CH-CH group of donors [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="acyl-CoA dehydrogenase family protein"
                     /protein_id="HWQ84984.1"
                     /translation="MNFDLTEEQQMWQRAVHGFVAQEVKPLAHTVDEEARFNWEAVHQ
                     MGPLGLLGLNVPEEYGGAGVDGVSAAIAIEELGWGCGSTALAIAAHNGLGCAPVALFG
                     SQKVKETFLPPAVTGKGNLAALALTEPGAGSDLKGGVTTRAEQIGNEWVINGPKMWCT
                     NASLADFIVTLARTGADMGSSSLSLIVVPTNSAGLQIGPAEKKMGLKGSPTNAVNYEN
                     VHVPLDYVLGEPGEGLHQTLTVLDGGRVGIAALAVGLAQAAYEEALRYAQERHTFGQS
                     IAGHQAIQWMLADAATEIQSARLMVRYAAWLKSEGRPFTQQASMAKLMATEMAERVCR
                     NAIQVHGGYGYSREFPVERIYRDARLMTIGEGTSEIQRMVIARHILKSH"
     gene            complement(1703..2305)
                     /gene="lepB"
                     /locus_tag="VN363_10485"
     CDS             complement(1703..2305)
                     /gene="lepB"
                     /locus_tag="VN363_10485"
                     /EC_number="3.4.21.89"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560324.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004252 - serine-type endopeptidase
                     activity [Evidence IEA];
                     GO_process: GO:0006465 - signal peptide processing
                     [Evidence IEA];
                     GO_process: GO:0009306 - protein secretion [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="signal peptidase I"
                     /protein_id="HWQ84985.1"
                     /translation="MENFETEIVPDPEPELPENNDSGSVRRFIIDVLETVVLSLVLFL
                     GINAISARIRIESISMQPTLFAGDYVIVNKVAYRLGEPTRGDVVVFQYPPDPGREPYI
                     KRVIGLPGDHVVIRNHQVVVNAVALNEPYINAAPNYDGEWRVPEDALFVLGDNRNNSS
                     DSHSWGMVPLENVIGKALVVYWPPSEWKALNSNNAVAAEP"
     gene            complement(2349..2888)
                     /locus_tag="VN363_10490"
     CDS             complement(2349..2888)
                     /locus_tag="VN363_10490"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560236.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0030151 - molybdenum ion binding [Evidence
                     IEA];
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="MogA/MoaB family molybdenum cofactor
                     biosynthesis protein"
                     /protein_id="HWQ84986.1"
                     /translation="MAEIDPPGSLRIGILTVSDRSARGERPDTAGPALAGAVAEQGWQ
                     VVQTEIVPDEMSVLVETLAAWCDSAQVDIVLTTGGTGFSHRDVTPEATLEIIERSAPG
                     LAEAMRSASLEVTPHAMLSRAVAGIRGRTLIVNMPGSPRAALENLAVILPVLPHAVEL
                     LREDAHAEAGHRKQDRTNR"
     gene            complement(2888..>3379)
                     /locus_tag="VN363_10495"
     CDS             complement(2888..>3379)
                     /locus_tag="VN363_10495"
                     /EC_number="2.3.1.-"
                     /inference="COORDINATES: protein motif:HMM:NF024871.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008080 - N-acetyltransferase activity
                     [Evidence IEA];
                     GO_function: GO:0016747 - acyltransferase activity,
                     transferring groups other than amino-acyl groups [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GNAT family N-acetyltransferase"
                     /protein_id="HWQ84987.1"
                     /translation="PMMGSTDRGNNSWGMKTIDQTMPRAAIRSFLYPEDLSGVLELWG
                     NAGPGVHLGRSDTPEEIHKKLAHDPDLFLVAEQEGRLVGAVLGGFDGRRGMVYHLAVA
                     SEQRRQGLGEALMQAVEARLREKGCLRYYLLVTQDNESAMKFYEQRGHDRLPLHIYAR
                     DLT"
BASE COUNT          802 a          921 c          888 g          768 t
ORIGIN      
        1 ggtacatact gggccgttcc gatgacacac tgaagatcgc cggcaaacgt ctcggaccgg
       61 cagaagttga atccattctg gtaaaccatc ccagcgtgat cgaagctgct gcaatcggaa
      121 ttccggatga aatcaaaggc aatgccctga tcgtttactg cgtattagcc gcggactgtg
      181 gtgcctgtga agagcttcgg catgagctgg ctgagctttt ggtccaccat cttgggaagg
      241 cactcgctcc caaggcgatt tattttgtgg cagatctgcc caaaacgcgc aatgccaagg
      301 ttatgcggcg catgatccgg gccgcttatc ttggtcagga tcccggggat acctcctcac
      361 tggtgaaccc agaggcagta gaggcaatcc gcaacagttt gtgaatgggt atgccgcctg
      421 gtaaaaaccc tacgtttgat tgccaggctg gataataatc cgtaaattct ggaataggag
      481 ggaaatatga atttcgacct gaccgaggaa cagcagatgt ggcagcgtgc tgtacatgga
      541 ttcgttgccc aggaggtcaa accgctcgcg catacggttg atgaagaagc ccgttttaat
      601 tgggaagctg tccatcaaat gggacccctg ggtcttttgg gccttaatgt tcccgaagag
      661 tacggcggtg caggagttga tggagtctca gcagcaattg caatcgagga gctgggttgg
      721 ggatgtggaa gcaccgccct ggcaattgca gcccacaatg gattgggatg cgcaccggtc
      781 gcacttttcg gcagccagaa ggtcaaagag acctttctgc cacctgcagt caccgggaaa
      841 ggtaatctcg ccgcgctggc gctcaccgag cctggcgctg gttcagacct caaaggaggg
      901 gtaacaacca gagctgaaca aatcggaaat gaatgggtta tcaatggccc caaaatgtgg
      961 tgcaccaatg ccagcctggc tgattttatt gtcacgcttg cacgcacagg cgcggacatg
     1021 ggttcgagct ccttgagtct gattgtagtg cctacgaatt ctgccgggtt gcaaatcggt
     1081 ccagcggaga agaaaatggg tctgaaaggc tcccctacca atgccgtcaa ttatgaaaat
     1141 gttcatgttc ctttagatta tgtgctgggt gagcctgggg aaggtttaca ccaaacctta
     1201 actgttctgg atggcgggcg ggtgggtatc gcggcgctgg cggttggcct ggctcaagct
     1261 gcctatgaag aagcgctgcg ctacgcacag gaaagacata cctttgggca aagcattgcc
     1321 ggacaccagg ctatccaatg gatgctcgca gatgctgcca cagaaatcca atccgcacgc
     1381 ctgatggttc gctacgctgc ctggctcaaa agcgaaggac gtccatttac acaacaggca
     1441 tccatggcca aactaatggc aacagaaatg gcagagcgcg tctgccggaa cgccattcaa
     1501 gtgcatggcg gctatggtta cagccgggaa ttccctgtag agcgtatcta ccgggatgcg
     1561 cgtttgatga caatcggcga aggtaccagc gaaattcagc gcatggtaat cgcccgccat
     1621 atcttgaaga gccattaaaa gagaaaaacc ggcatctgat agtattcagg tgccggtcag
     1681 attctcttaa ttcagaatcg ggtcagggct ctgctgccac cgcgttgttg gaattcaaag
     1741 ctttccattc tgatggcggc cagtatacca ccagtgcttt cccaatcaca ttctccaaag
     1801 gcaccattcc ccagctgtgt gaatcggaag aattgttccg gttatcgccc agcacaaaca
     1861 gcgcgtcttc cggtacccgc cattctccat catagttggg cgcggcattg atatacggct
     1921 cattcagagc aacagcgttg accactacct gatggttgcg aatgacgaca tgatctcctg
     1981 gcagaccgat gacacgtttg atatagggct ctcgtcctgg atcaggtggg tattgaaaga
     2041 ccaccacatc accccgggtc ggttcgccca gcctgtaagc aactttgtta acgatcacat
     2101 aatcacccgc aaataaagtg ggctgcatac tgatcgattc aattcggatg cgagctgaaa
     2161 tggcgttgat ccccaaaaac aggaccagag ataacaccac agtttccaaa acatcaatga
     2221 taaaacgccg gacacttccg ctgtcgttat tttcgggaag ctccggttct ggatcgggga
     2281 ctatttccgt ttcaaaattc tccatcctga tctattccta aagctcaata atgcagcttg
     2341 tttgattctt agcgatttgt tcgatcttgt ttccggtggc ctgcttcggc atgcgcgtcc
     2401 tcgcgcaaca gctcgactgc atgcgggagt actggcagga ttaccgccag gttctcgagt
     2461 gctgctcgcg ggctgccagg catattaacg atcaatgttc gaccacgaat tccagcaacc
     2521 gcccgcgata acatggcatg cggggttacc tctaaactgg cgcttcgcat tgcttccgca
     2581 aggccgggag cgctgcgctc aatgatctcc agcgtcgctt cgggtgtcac gtctcggtga
     2641 ctgaaacctg ttcctccggt cgtcagtacg atatccactt gcgccgaatc gcaccaggct
     2701 gccagagttt caaccagaac gctcatctcg tcaggcacaa tctcagtctg gacgacctgc
     2761 cagccctgct ccgcgacggc tccagctaag gctggaccag cagtatcggg gcgttcgccc
     2821 cgcgccgagc ggtccgagac ggtcagaatc ccaatccgta atgagcctgg tggatctatc
     2881 tcagccatca agttaaatcc ctggcgtaaa tatgcaaagg cagacgatcg tgtccacgct
     2941 gttcataaaa tttcatcgca ctttcgttat cttgggtgac gagcaaataa tatcgcaagc
     3001 agcctttttc tcttaagcgg gcttcaacag cctgcatcaa tgcctcaccc aacccctggc
     3061 gacgttgttc actcgcaacg gcaagatgat acaccatccc acgacgcccg tcaaacccac
     3121 ccaatacggc tccgaccaga cgaccttcct gctcggcgac cagaaacaga tccggatcgt
     3181 gggcaagttt cttatggatt tcctcaggag tatccgagcg cccgaggtgc acacctggcc
     3241 cagcgttacc ccaaagctcc aggactccgc ttaaatcttc cggatacaga aatgaacgga
     3301 tcgctgctcg tggcatcgtt tgatcaatcg tcttcatgcc ccaggagttg tttccacggt
     3361 ccgtggaccc catcattgg
//
LOCUS       DATMXK010000576         2019 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_53062, whole genome shotgun sequence.
ACCESSION   DATMXK010000576 DATMXK010000000
VERSION     DATMXK010000576.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2019)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2019)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2019
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_53062"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            32..367
                     /locus_tag="VN363_10500"
     CDS             32..367
                     /locus_tag="VN363_10500"
                     /inference="COORDINATES: protein motif:HMM:TIGR00377.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0043856 - anti-sigma factor antagonist
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="STAS domain-containing protein"
                     /protein_id="HWQ84988.1"
                     /translation="METTLTQFKHCDSVKVTGRIDSATAPKFAEALDSILNEGRHKIV
                     LDLEKVEFMSSAGLRVLISAQKVCRRYNRGEVVLALVPQRVYEAMDLAGFVPLFKFFD
                     DVTTAVGSF"
     gene            489..950
                     /locus_tag="VN363_10505"
     CDS             489..950
                     /locus_tag="VN363_10505"
                     /EC_number="2.7.13.3"
                     /inference="COORDINATES: protein motif:HMM:NF024970.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000155 - phosphorelay sensor kinase
                     activity [Evidence IEA];
                     GO_function: GO:0004673 - protein histidine kinase
                     activity [Evidence IEA];
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_process: GO:0007165 - signal transduction [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ATP-binding protein"
                     /protein_id="HWQ84989.1"
                     /translation="MSSSETFFETRQAKYTAQFENLDDMRVFVGNAARDSGLESAEIY
                     AVQLAVDEASSNIIEHAYGGESEELIECTCTIKPGSLKVTLKDCGHHFDPSEVREPDL
                     TSDIEERQPGGLGLYFMRQLMDEVSFTIVPGKGEQKDCNILTMIKHKEGRG"
     gene            959..>2019
                     /locus_tag="VN363_10510"
     CDS             959..>2019
                     /locus_tag="VN363_10510"
                     /inference="COORDINATES: protein motif:HMM:NF024583.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005515 - protein binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="GAF domain-containing protein"
                     /protein_id="HWQ84990.1"
                     /translation="MSTEHPSRWRQILILGERLQEISISAAAGQGDDSSPHSAATDAV
                     TVLNAQQNEIISQTGQLLACQTKLWLSEGLLPSRTWHPDQGSEQRGLGDLCTPVMLAA
                     YQTGQIRCVEEANDPDVFSGPECQETRSQYPIVAVPILSQALSSEILGVLQVERLPGT
                     GFTPDDIALLEGIASQVGIGLQAARRLASERWRVEQLALVQHVSTQIVNVRDLEELTR
                     TLTSLIQSTFGYYYVAIFTVEPGQESLNFQASSGGEPHPGSSVRRPTSEPLFVHVGKG
                     LIGQVAQTGQEIVANDVTQEPRFLDSDLLPDTRAEASFPLKIEDRVVGVLDVQSDYPG
                     AFQEVDMLVLRALAGNIAIA"
BASE COUNT          488 a          486 c          571 g          474 t
ORIGIN      
        1 atcgggaaaa ttgccaagcg gaggtccaat catggaaaca accctgaccc aattcaagca
       61 ctgcgactcg gttaaagtca caggccggat cgatagcgcc acagcaccaa agtttgcaga
      121 agcccttgat tcaattctga atgaagggag gcacaaaatt gtcctggatc tggaaaaagt
      181 cgagtttatg tccagtgcag ggttgcgcgt attaatcagc gctcaaaaag tgtgccggcg
      241 gtacaaccgg ggtgaagtag ttttggcgtt ggtgccgcaa cgtgtgtatg aagcgatgga
      301 tttagcaggc tttgtcccac tcttcaaatt cttcgacgat gtcaccactg ccgtgggaag
      361 tttctaaatt ggccagggta aacgtcagga agccaattac tgacagatca tgaccggaat
      421 attcgaagag gaatgtcgct aaggctagct aagccctaat cgcattcctc tttttttaca
      481 ggtaaagcat gagtagctcc gagacattct tcgaaacccg ccaggcgaaa tatactgccc
      541 agttcgaaaa cctggatgat atgcgcgtat ttgtgggcaa tgcggcccgc gattccgggt
      601 tggagtccgc tgagatttat gcggttcaac tggctgttga tgaagcctcc tcaaatatta
      661 tcgagcatgc ctatggcggc gaatcggaag agctcataga atgtacctgt acgatcaaac
      721 ctggaagcct taaggttacc ttgaaggatt gcgggcatca ttttgaccct tctgaggtca
      781 gagaacctga ccttacttcg gatattgaag aacgccagcc tggtggtttg ggcctgtatt
      841 ttatgcgcca gcttatggat gaggtctcat ttacaattgt gcctggcaag ggggagcaaa
      901 aagactgtaa tatcctgacc atgatcaagc acaaagaagg ccggggatga ggggtggaat
      961 gtcaacagaa catccaagtc gctggcgaca gatacttatc ctgggtgaac gccttcaaga
     1021 aataagcatt tctgctgcag ccggtcaggg ggatgactcg agtccgcatt cggcagccac
     1081 ggatgccgtg actgtattga acgcccaaca gaatgaaatc atctcccaga ccggacagct
     1141 attagcttgc cagacaaaat tgtggctttc tgaagggctt ctccccagtc gtacctggca
     1201 tcccgatcaa gggagtgagc agcgtgggct tggtgacctc tgcacaccgg tcatgctggc
     1261 ggcttaccag actggccaga ttcgctgtgt ggaagaggcc aatgacccgg atgtcttcag
     1321 cggcccggaa tgccaggaaa cccggtctca atatccaatt gtggctgtcc ccattttaag
     1381 ccaggcgctg agcagtgaaa tcctgggtgt gctgcaggtt gagcggctac cgggaacagg
     1441 gtttaccccg gacgatatcg ccctgttaga ggggattgcc tctcaggttg gcattgggct
     1501 gcaggctgca cgccgtctgg catctgaacg ctggcgggtg gaacagctgg ctctggtgca
     1561 gcatgtcagc actcaaatcg tgaatgtgcg tgacctggag gagctgactc gcacgctgac
     1621 cagcttgatc cagagtacat ttggctacta ctatgtggca atctttacag ttgagccggg
     1681 ccaggaaagc ttaaattttc aggccagttc tggcggagag ccacatcccg gttcaagcgt
     1741 ccgccgcccg acgagtgagc ctttattcgt tcatgttggg aagggattga ttggccaggt
     1801 tgcccaaacg ggccaggaaa ttgtcgccaa tgatgtgacg caagaaccgc gctttctgga
     1861 ttcagatttg ctccccgata cgcgtgccga agcttcgttt ccactgaaaa ttgaagatag
     1921 ggttgtgggt gtcctcgatg tgcagagcga ttatcccggc gcgtttcaag aagtagatat
     1981 gctcgtgctg cgcgccctgg ccgggaatat agcaattgc
//
LOCUS       DATMXK010000577         2212 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_5852, whole genome shotgun sequence.
ACCESSION   DATMXK010000577 DATMXK010000000
VERSION     DATMXK010000577.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2212)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2212)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2212
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_5852"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..1152
                     /locus_tag="VN363_10515"
     CDS             <1..1152
                     /locus_tag="VN363_10515"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560740.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="ribonucleoside-diphosphate reductase,
                     adenosylcobalamin-dependent"
                     /protein_id="HWQ84991.1"
                     /translation="GILTHGIRNAAQTTVAPTGTIATVAGCEGYGCEPVFALAYIRHV
                     NDKGKDLQLTYTSPLFEQALISSGLSEAARAEIVEYAMSHGTCQDYPGVPEAIKHTFV
                     VSQDITAEEHVRMQAALQVFVDNSLSKTINFPPDATEEDVATAYQLAWELGCKGITVY
                     ITGSRQKVVLETHATAKEKEPTAAPTVMTVPVPQLSGEQLPIWHDKKKPRPRSLRGYT
                     YTVSTPLGKAFVTINENGGDQPFEVFINTAKAGSDTAAVSEAIGRLISYTLRLASPIP
                     SRDRLAEVHRQLSGIGGGRSLGFGPNRVRSLPDGVAQVLVEYLDATQGEHTPDKPDSE
                     RDNGYHLLAEQPQAPLSIKIGDLCPECGQAAVVNEEGCRKCYACGFSEC"
     gene            1206..1670
                     /locus_tag="VN363_10520"
     CDS             1206..1670
                     /locus_tag="VN363_10520"
                     /inference="COORDINATES: protein motif:HMM:NF039927.3"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF5946 family protein"
                     /protein_id="HWQ84992.1"
                     /translation="MLPESSCPECNSPLQDGLTCQDHFHTLLFWENEVPEYGEVHHLT
                     VLCYHLQHPSLYSPEGLIGARQLLTDFLVLGLSPSQVVRRDREIRDSGKRQSRITSRL
                     GQAGSYPVPVKWTMTAADVVAAGKENYIPTVPSWAFTVKQALDEVPPFRETQ"
     gene            1792..>2212
                     /locus_tag="VN363_10525"
     CDS             1792..>2212
                     /locus_tag="VN363_10525"
                     /inference="COORDINATES: protein motif:HMM:NF013188.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0015297 - antiporter activity [Evidence
                     IEA];
                     GO_process: GO:0006812 - monoatomic cation transport
                     [Evidence IEA];
                     GO_process: GO:0055085 - transmembrane transport [Evidence
                     IEA];
                     GO_process: GO:1902600 - proton transmembrane transport
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="cation:proton antiporter"
                     /protein_id="HWQ84993.1"
                     /translation="MQQFLTTETLIIELLLIASLVAIVVRRLHIPYTVALVVVGLLLT
                     AQSSVRIELTPELILALFVPPLIFEAAFHLKLSELRQNLAYILVLAVPGVILTTLIVA
                     GTLVLGTDLTLPIALVFGSLIAATDPVAVVALFRLLGV"
BASE COUNT          505 a          643 c          559 g          505 t
ORIGIN      
        1 ggtatcctca ctcatgggat ccgcaatgcc gcccagacta ctgtcgctcc cactggcact
       61 atcgccacag tcgccggatg tgaaggatat gggtgcgagc cggtatttgc cctggcatac
      121 atccggcatg tcaacgacaa aggtaaagat cttcagctta catataccag tccactattt
      181 gagcaagccc tgatttccag cggcttgagc gaagccgccc gcgcagaaat cgttgagtat
      241 gcgatgtctc acggcacctg ccaggattat cccggcgtgc cagaagcgat aaaacataca
      301 tttgtcgtgt cacaggacat cactgccgag gaacatgtac gcatgcaagc cgcgctgcag
      361 gtcttcgtgg ataacagcct cagtaaaacg atcaatttcc cgcccgatgc caccgaagaa
      421 gatgtagcga ctgcgtacca attggcctgg gagctgggct gcaagggtat cacggtctac
      481 atcaccggat cgcgccagaa agtcgtcctg gagacacatg ccactgccaa agaaaaggaa
      541 cccactgctg ccccaaccgt aatgacagtc ccggtcccac agctatccgg tgagcagctg
      601 cccatctggc acgacaagaa gaagccgcgc ccgcgcagcc tgcgcggcta cacatacacg
      661 gtcagtactc cgcttggaaa agcttttgtg acaatcaatg agaatggtgg cgaccagcct
      721 tttgaagttt tcattaatac tgccaaagcc ggctcggata ctgcagcagt ctctgaggcg
      781 attggacgct taatctccta taccctgcgt ctggcttctc cgatcccctc ccgtgaccgt
      841 ttagcagagg tccaccgcca gctctccggc attggagggg ggcgctcgct aggcttcggg
      901 cctaaccggg tgcgctctct gccagacggg gtagcccagg tgctggttga gtacctggac
      961 gccacccagg gagagcacac tcccgataaa cccgatagcg aaagggataa cggctaccat
     1021 ctattagccg agcagcctca agccccatta agtattaaaa ttggcgacct gtgccctgaa
     1081 tgtgggcagg cagcagttgt aaacgaggag ggttgccgga agtgctacgc ctgtgggttt
     1141 agcgagtgct gaaagatatt tgatgtggac agggtatcca tccccatacc ctgtccacgg
     1201 ctctcatgct gcctgaatca tcctgccccg agtgcaacag ccccttacag gatggtctca
     1261 cctgccagga ccacttccac acgctcctct tctgggagaa cgaagtgcct gaatatggtg
     1321 aagtacatca tcttacggtc ttgtgctacc atctccaaca ccctagcctg tattctcctg
     1381 aaggcctgat cggtgcccgc cagctgctta cagacttcct ggtactgggt ctcagcccga
     1441 gtcaggtagt tcgccgggat agagagatcc gagactcagg caagcgccag tccaggatca
     1501 ccagccgcct gggacaggca ggctcctacc ccgtcccggt taaatggacg atgacagcag
     1561 cagatgtggt agctgccggt aaagagaatt acattcctac cgtgccttca tgggcattca
     1621 ccgttaaaca ggcgttagat gaagtgcctc cattcaggga aacacaatag gcaatctctc
     1681 aattcgcctt gtaaccagcg ggaaatatat taaactaatc ctcggaacca ttcgtgccga
     1741 cacggcagga atattttatt gctttgctgt gagaaaggcc gggcgcttgc tatgcagcaa
     1801 ttcctgacaa ccgaaaccct cattattgaa ctcttactga ttgcatctct ggtagccatc
     1861 gttgtcaggc ggcttcatat accctatacg gttgctctgg ttgtagtcgg cttactcctg
     1921 accgcccaat cctccgtccg gatagaatta accccggaac tgatcctggc gctattcgtg
     1981 ccgccattaa tattcgaagc cgcattccat ttgaaattat ccgaattacg tcaaaacctg
     2041 gcttacattc tcgtcctggc agttccagga gtaatcctga cgactcttat tgtagctgga
     2101 acgctggtcc tgggtaccga ccttactctg ccgatcgcgc tggtctttgg ctcccttatc
     2161 gctgctacag atcctgttgc ggttgttgct ctgttccgcc ttctcggtgt ac
//
LOCUS       DATMXK010000578         2088 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_17723, whole genome shotgun sequence.
ACCESSION   DATMXK010000578 DATMXK010000000
VERSION     DATMXK010000578.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2088)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2088)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2088
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_17723"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..234)
                     /locus_tag="VN363_10530"
     CDS             complement(<1..234)
                     /locus_tag="VN363_10530"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ84994.1"
                     /translation="MMEKIRVVFNNLQQRRKLLTLTSGGLILLALGADFFFAWRSVYN
                     FGMVAAALVAGWDIAQRALISLRNRHVSIELLVT"
     gene            complement(287..505)
                     /locus_tag="VN363_10535"
     CDS             complement(287..505)
                     /locus_tag="VN363_10535"
                     /inference="COORDINATES: protein motif:HMM:NF012621.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0046872 - metal ion binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="heavy-metal-associated domain-containing
                     protein"
                     /protein_id="HWQ84995.1"
                     /translation="MKKTLLRSQELSCPSCVAKIETALKALEGVNEAKVFFNTGKIEV
                     QHDPELVKAEELVKAIKSVGYEAKVAAF"
     gene            complement(655..1413)
                     /locus_tag="VN363_10540"
     CDS             complement(655..1413)
                     /locus_tag="VN363_10540"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWQ84996.1"
                     /translation="MALRRKTPLRTGYTEVHQCSVDVRLSILGQLPFFEDLSQADLTV
                     INQYFREIGFTANETICFAGDPAERLFVVADGRVKLMHSTQSGKDVLLDLLTQGEFFG
                     SLSVLGEDVYPDTAVAQTQVCILVIEKDAFMQILTRFPSVPLKVIGIMAERLRAANER
                     LERMSVGTVEERIAHGLLRLSDKFGEKKRGVGLLIQVPLTRDDLAALTGATTETASRV
                     MSQLQKDGLIQSGRGWVAIRDLKGLQEIAGVEAP"
     gene            complement(1397..>2088)
                     /locus_tag="VN363_10545"
     CDS             complement(1397..>2088)
                     /locus_tag="VN363_10545"
                     /EC_number="3.1.3.-"
                     /inference="COORDINATES: protein motif:HMM:NF012521.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="histidine phosphatase family protein"
                     /protein_id="HWQ84997.1"
                     /translation="QKTTRLYLVRHGATQLSAEDRFAGSIDPELSETGIYQAQRLAER
                     LADDEISTVYCSPMIRTIQTATILAKPHDIIPIQRDGLREINHGHWEGCLRGEVEQLY
                     PEEYAAWEEDPFTFAPQGGEAGVNVIARALPVIREIVVNHPGQNVIVVTHKATLRLLI
                     SSILGFDARGYRDRLDQSPAALNVVDLKDPVRARLMLFNDVSHYKDQPARSDGRLSKW
                     WDTNTSNGSAA"
BASE COUNT          463 a          602 c          536 g          487 t
ORIGIN      
        1 cgttaccagc agctcgatgc tgacatgccg gttgcgtaag ctgatcaatg cccgctgagc
       61 aatatcccaa ccagccacaa gcgccgcagc caccatgccg aaattgtaaa cactccgcca
      121 ggcgaagaaa aagtcagcgc ctaatgcgag caggatcagc cctccactgg ttaatgtcag
      181 cagcttgcgt cgctgctgca ggttattgaa gaccacgcgg attttttcca tcattcactt
      241 catccaaaat agttcgatgt ttcgaaattc agggataatc ggttacctaa aaggctgcta
      301 ctttggcctc atatccaacc gacttaatag cctttaccag ttcttcggct tttacaagtt
      361 caggatcgtg ctggacttcg atcttgccgg tgttgaagaa cactttggct tcgttcactc
      421 cctccaatgc ctttaaagcg gtttcgatct tcgccacgca cgatgggcag gatagttcct
      481 ggctgcgtaa caatgtcttt ttcatgggta attctctcct ttcaaaataa atcgctcaac
      541 ttgatgacat aaatatactg gttcggcgag tttcgcacca tgatctggcg caattaagtg
      601 caattaaata aaaaagtgcg gcttgtgacc gcacttttcc ggagagcaat ctattcaagg
      661 cgcttctaca ccagcaattt cctgcagccc tttcagatcg cggatcgcca cccagcccct
      721 cccggactgg atcaggccat ccttctggag ctggctcatc acccggctgg cagtctcggt
      781 ggtcgctccg gtcagtgccg ccaggtcgtc gcgagtcagc ggaacctgga tcaataaccc
      841 gaccccgcgc ttcttttcgc caaatttgtc gctcaagcgc agaagtccat gggcgatccg
      901 ttcttcgact gtacccacgc tcattcgctc cagccgctca ttggcagccc tcagtctctc
      961 cgccataatc ccgatcacct tgagcggaac ggagggaaag cgggtgagaa tctgcatgaa
     1021 ggcgtctttt tcgatcacca ggatgcacac ctgggtctga gcaacagcag tatccggata
     1081 aacatcctcg cccagcaccg agagcgagcc gaagaattcg ccctgggtga gcagatcgag
     1141 cagcacatct ttacccgact gggtgctgtg catcaatttt acccgcccat cggcgacgac
     1201 aaacaaccgc tcggcgggat cgccggcaaa gcagatcgtc tcgttcgctg tgaaaccgat
     1261 ttcccggaaa tattggttaa tgacagttag atcagcctgt gagagatcct caaaaaaggg
     1321 cagctgcccc aggatagaca ggcgcacatc caccgagcat tggtggactt cggtataccc
     1381 ggttcggagt ggggttttac gccgcagagc cattactggt attcgtatcc caccactttg
     1441 agagcctgcc gtccgagcgc gccggctgat ctttataatg ggaaacatcg ttgaataaca
     1501 tcagccgcgc ccgcaccggg tctttgaggt cgaccacatt cagggcggcc ggggactggt
     1561 cgaggcgatc gcggtaaccg cgggcatcaa agccgagaat gctgctgatc aacaaacgca
     1621 aggttgcttt gtgagtgaca acaattacgt tctgcccggg atggttgacc acgatctcgc
     1681 gaatgaccgg cagcgccctg gcgatcacgt tcaccccggc ttcgccgccc tgcggtgcaa
     1741 acgtgaatgg gtcttcttcc caggctgcgt actcttcagg atatagctgt tcaacttcgc
     1801 cgcgcagaca gccttcccaa tgcccatgat tgatctcacg caaaccatcc cgctggatcg
     1861 ggataatgtc gtgcggtttt gccaggatcg tcgccgtctg gatggttcgg atcatcgggc
     1921 tgcagtatac ggttgagatt tcatcatctg ccaggcgctc tgcgaggcgc tgcgcctgat
     1981 agatgcccgt ttccgaaagc tccgggtcaa tcgagccggc aaaacgatct tcggcgctga
     2041 gctgagtcgc gccgtggcgt accaggtata aacgcgtggt tttttgtt
//
LOCUS       DATMXK010000579         2618 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_168394, whole genome shotgun sequence.
ACCESSION   DATMXK010000579 DATMXK010000000
VERSION     DATMXK010000579.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2618)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2618)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2618
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_168394"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..578)
                     /locus_tag="VN363_10550"
     CDS             complement(<1..578)
                     /locus_tag="VN363_10550"
                     /inference="COORDINATES: protein motif:HMM:NF023069.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DnaA N-terminal domain-containing protein"
                     /protein_id="HWQ84998.1"
                     /translation="MNGSNIQPERAWQMALDQLRLDMPKASFDTWVRDTSFMSFEDGV
                     FTIGTTNAYGREWLASRLTSTVTRLMSGILNLQMVVEFIVTEEILENEEDGFLEEEIP
                     VPDKHPTVLSIQAEYQSIYDEIVQPDHVIVVPGYFMRYIPLLGLELAWLYIGFRQSAY
                     EAGAARQPGKKFGAPSKKVAHFSGMSTRTFWR"
     gene            complement(575..832)
                     /locus_tag="VN363_10555"
     CDS             complement(575..832)
                     /locus_tag="VN363_10555"
                     /inference="COORDINATES: protein motif:HMM:TIGR01764.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="helix-turn-helix domain-containing protein"
                     /protein_id="HWQ84999.1"
                     /translation="MTTQSTHPPPGTWNARNLLTVLEVAEWARVHPKTVYRWIKEGKL
                     EAIQFGPRTFRVPEDAIGKFLHKTGYSKTTALPKPNGREQS"
     gene            complement(1158..>2618)
                     /locus_tag="VN363_10560"
     CDS             complement(1158..>2618)
                     /locus_tag="VN363_10560"
                     /inference="COORDINATES: protein motif:HMM:NF019149.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0000150 - DNA strand exchange activity
                     [Evidence IEA];
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_process: GO:0006310 - DNA recombination [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="recombinase family protein"
                     /protein_id="HWQ85000.1"
                     /translation="PGINAMREAARRGEFKVLYVHKVDRLARRLEWSLEIVHELQELD
                     IIFKAVQQPLDLGTPEGKLLFHLVSSLGEFYSDNLSKETNKGKLERSLQGYHNGAVPW
                     GYISVLQGNRKVGVPDPGKASVVVEMFERYATGLYSDMQIAAWLNEGEYLTAKGRSFN
                     KDSVRDMLCNPYFVGKIRYRGMTVRPKGVSYRSTPPQVTEGQHEPIITQELWDRCQGL
                     RASRRVVPKTGQKAARVHLLQSLAVCAYCGRRLRIQTPKNFPTYYREDSHLRGYHDCP
                     FSGQSIHADKLDEQVAELIQALKLTATWEEDVRKLLHEEQDRPDPETERKEIRSMLRL
                     MRDNYERGMYEGEEYQYWQKVNGLKEKLALLERVPEPALNRAARTLLDLRETWESTTK
                     EERKDLVHIMIQEVGVDVVNKRVLWVKARPDYELLFSILDGMRQDADRRFWIEQMEPQ
                     EDNCDIEEDTGQVSTGVEIVLQMSHNILTRTEEYVQ"
BASE COUNT          540 a          738 c          692 g          648 t
ORIGIN      
        1 cagcgccaga atgtccgtgt actcatccca gaaaagtggg caaccttctt ggatggtgcg
       61 ccgaatttct ttccgggttg tctggcagcg cccgcttcat aagctgactg gcgaaaacct
      121 atgtacagcc aggctaactc caatcctaga agtgggatgt atcgcatgaa gtaccccgga
      181 acaacaatga cgtggtctgg ctgaacgatt tcgtcgtaga tcgactggta ctccgcctgg
      241 atggacagca cagttgggtg tttatcagga actggaattt cttcctcaag aaaaccatcc
      301 tcctcgtttt caaggatttc ttcggtgaca ataaactcga ctaccatttg tagatttaag
      361 atgccggaca tcagacgcgt cacagtactg gtcaaacggc ttgccagcca ctctcggccg
      421 taggcattgg ttgtgcctat ggtgaaaacc ccatcctcga atgacataaa gctggtgtca
      481 cgcacccagg tatcgaaaga agctttcggc atatccaaac gcagctggtc aagcgccatt
      541 tgccacgccc tctccggttg gatattggag ccgttcatga ttgctctctc ccattgggtt
      601 tgggtagtgc agtggtcttg ctgtaaccgg ttttgtgtag aaacttgcca atggcatctt
      661 ctgggacacg gaaagtccgg ggtccaaatt ggatggcttc aagcttgcct tccttgatcc
      721 agcggtaaac cgttttaggg tggacacgtg cccattccgc cacttcgagc acggtcagta
      781 agttgcgggc attccatgtg cccggcggtg ggtgtgtaga ttgtgtagtc atctccttat
      841 ctcccgccga ctttttggga gaatcaaaaa gccggctctc aatctggcct gtgaaaacac
      901 aggttcagat cgagagccgg ctaataattg cttttagcct actttacgct cggatgagcg
      961 ggcacccacc ctctccgcag gtcggaagat cctcatagca gcggagccgc tttgtggctt
     1021 agtctgcaca ttatcgccgg cctcaatcgc ttggtgatga tggactcaaa tgactgaggg
     1081 cttgcgactg gcgtgatttt cacgcccgag atcgctcagt gtccgctggg cgatctcact
     1141 tttaaagtgc ggtaccctca ttgtacgtac tcctctgttc ttgtcaagat attgtgggac
     1201 atctgcagca cgatttcgac accggtactc acttgtcccg tatcttcttc tatgtcacag
     1261 ttgtcctctt gcggttccat ttgctctatc cagaaccgcc tatcggcatc ctgacgcatc
     1321 ccatccagaa tagagaagag tagctcgtaa tccgggcggg ctttcaccca tagaacacgt
     1381 ttattcacca cgtcaacgcc aacttcctgg atcatgatgt gaacaagatc cttgcgctct
     1441 tctttggtgg tgctttccca agtttcgcgt aagtcgagca gggtccgagc cgcccggtta
     1501 agggcaggct caggaacacg ctccagcaat gccagttttt ccttcaagcc gttgaccttc
     1561 tgccagtact ggtattcttc gccttcatac atgccacgct cgtaattgtc acgcatcagg
     1621 cgtaacatgc tgcggatctc cttgcgctca gtctctgggt caggcctatc ctgttcctca
     1681 tgcagcagtt tacgcacatc ctcttcccag gtggcagtca acttgagggc ctgaatcagc
     1741 tcggcaacct gttcgtccag cttatcagca tgaatgctct gacccgagaa cgggcagtcg
     1801 tggtagccgc gcaggtgcga atcctcccgg tagtaggtgg gaaaattctt gggtgtctgg
     1861 atacgaagtc gccgaccaca gtatgcacag accgccagac tttgcagcag gtgaaccctg
     1921 gctgccttct gtccggtttt ggggaccaca cgccggctgg cacgtagccc ctgacagcga
     1981 tcccacaact cctgggtgat gatcggctca tgctggccct cagtgacctg gggcggggtg
     2041 gaacggtagc tgacaccctt ggggcgtacc gtcatgccgc ggtaacggat tttaccgaca
     2101 aagtagggat tgcagagcat atcccgcacc gagtccttgt tgaaggagcg gcctttggca
     2161 gtgaggtatt ctccttcgtt caaccaggca gcgatctgca tgtccgaata aagcccggtg
     2221 gcgtaacgct cgaacatctc caccaccacc gaggccttcc ccgggtcggg aacgcccact
     2281 ttgcgattgc cctgcagcac gctgatgtac ccccagggga ccgccccgtt gtgatagcct
     2341 tgcagcgaac gctccagctt acccttgttg gtctctttgg ataagttatc gctatagaac
     2401 tcgccgagcg agctgaccag gtgaaagagc agcttgcctt ccggtgtgcc cagatcgagg
     2461 ggctgttgga cagccttgaa gatgatatcg agctcttgca gctcgtggac gatctccaga
     2521 gaccattcca ggcggcgcgc caggcgatct actttgtgga cgtacagcac cttgaactcg
     2581 ccgcgccggg cagcttcgcg catggcattg atccctgg
//
LOCUS       DATMXK010000580         4062 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_51795, whole genome shotgun sequence.
ACCESSION   DATMXK010000580 DATMXK010000000
VERSION     DATMXK010000580.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 4062)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 4062)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..4062
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_51795"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(25..399)
                     /locus_tag="VN363_10565"
     CDS             complement(25..399)
                     /locus_tag="VN363_10565"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558807.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="roadblock/LC7 domain-containing protein"
                     /protein_id="HWQ85001.1"
                     /translation="MTHSRTELMVSRLREMQAASPEIEASAVVSVDGLIMASALPAEV
                     EEDRVSAMSAAMLSLGERIAKELGRGNLEQVYIRGNSGYVILSAVGEEAVLTALAREG
                     AKLGLIFLEMRRAAESLERMVG"
     gene            584..2422
                     /locus_tag="VN363_10570"
     CDS             584..2422
                     /locus_tag="VN363_10570"
                     /inference="COORDINATES: protein motif:HMM:NF024910.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0016020 - membrane [Evidence IEA];
                     GO_function: GO:0022857 - transmembrane transporter
                     activity [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="APC family permease"
                     /protein_id="HWQ85002.1"
                     /translation="MQYKIKHLLIGSPLSNEQSGEKRLNKIRALAAFSPDALSSIAYA
                     NQEIYLGLIVAGAAGLSLSLPIGLTIAGLLIIVAISYYQTIHGYPQGGGSYIVTRENL
                     GRMPSLIAGAAILVDYLLTAAVSITAGVEALASAFPVLWGHRVLVSLGILLVITLINL
                     RGLKEAGTFMTMPVYLFLFTYLPLLAYGAVRLFIDGPRDIQAVAPAASQPLAAFLILR
                     AFASGCTALTGIEAISDGVPAFRPPQAKNAGKTLAVMAVLMFILFVGSISLIYSLAVV
                     AGPQETILSALARRLLGSGPLYILIQASTMLILTVAANTSYADFPRVASFLARDGFLP
                     RQLANLGDRLVFSNGILLLAAGTAALIIAFQGDTHSLVPLFAIGAFLAFTLSQSSMVM
                     HWMRERGPNWKIKAFANGAGAIATGTTLLVVAVSKFQQGAWLTILVIPTAVIIFLRIH
                     AHYQTVAAQLTLRGLPPSLKPQGTMRAVIPVSGVHRGMIDAVLFAQTIARQVTGVYIE
                     LNPEASANVQKMWQRWFPDIPLVVLPSPYRSIVEPLLKFLDETDREHNDGQMAVVVLP
                     EFVPARWWQSLLHNQTSRLIKNALLYSRREKGYQRVIIDVPYHLKR"
     gene            2510..3172
                     /locus_tag="VN363_10575"
     CDS             2510..3172
                     /locus_tag="VN363_10575"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ85003.1"
                     /translation="MIFLVLFFLLHTRAAPAYAQALTPTAPAGTNIPSPTIGVSAPVA
                     GQALQGVIAITGNSAIPEFSRAELSFAYATDTTGTWFLIAESQTPVENGTLGQWDTTT
                     ITDGEYQLRLVVYLTGGQKQIATNTGLRVRNYTAVETETPTPSATMRPGNTPAPSNTP
                     TPTSTPVPPTGTPVPPNPAIIGPGDLLRNMGRGALGIVGIFSLIGIYRGLHSLIQKRS
                     RQ"
     gene            3252..3863
                     /gene="pth"
                     /locus_tag="VN363_10580"
     CDS             3252..3863
                     /gene="pth"
                     /locus_tag="VN363_10580"
                     /EC_number="3.1.1.29"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560439.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0004045 - aminoacyl-tRNA hydrolase
                     activity [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="aminoacyl-tRNA hydrolase"
                     /protein_id="HWQ85004.1"
                     /translation="MEPENSAGIPYLIVGLGNPGREYRTTRHNMGFMLLDRLAERLGV
                     TFSRLESQALVAKTEHAGSRIILAKPQAYMNLSGQPVGALLHYYKIPVSQLLVAYDDV
                     DLPLGSLRIRHGGGSAGQKGMASIIERLGTQEFPRLRLGIDRPPGRMQAAAYVLQEFS
                     SLQKPVVTTALDRAADAVLLFISDGLDAAMNRYNGTGTPNGSQ"
     gene            3850..>4062
                     /locus_tag="VN363_10585"
     CDS             3850..>4062
                     /locus_tag="VN363_10585"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ85005.1"
                     /translation="MALSDLIESIAPHFEPVFADLQAEQNLSALGLRRAARLPFLAAL
                     HREMQRPIIFLTDRTDRALAMQEELSL"
BASE COUNT          917 a         1130 c         1032 g          983 t
ORIGIN      
        1 gtagagaacg gtccagcaca tcggctagcc aaccatccgt tccaggctct ccgcagcgcg
       61 gcgcatttca aggaaaataa ggcccagttt ggcgccttcg cgggccagag ctgtaagcac
      121 tgcttcctct ccgactgccg aaagaatgac ataacccgag ttgccgcgga tgtaaacctg
      181 ctccagattg ccacgaccca attcttttgc aatccgctcg ccaaggctca acattgctgc
      241 tgacatcgcc gagacgcggt cttcctctac ctcggctggc agcgctgaag ccatgatcaa
      301 cccatctact gaaacaaccg ctgaggcttc aatttccggt gacgcggctt gcatttcccg
      361 cagccggctg accatcaatt ctgtgcgcga gtgtgtcatt ggcaggttcc ctttcattgt
      421 gtgctttcgc gatggctagg ctgagtctac catatttttt atgcttaggg caaaacacca
      481 atccactgag taggagatcc tggatgatag gagagtaaat taattgagaa tatatcaggg
      541 atcatcacta taatagggta tcttgatttg aggacaggag tctatgcaat acaaaatcaa
      601 gcacctgtta atcggcagcc ctctatcaaa cgagcaaagc ggtgaaaaac gcctgaataa
      661 gattcgcgcc ttggccgcat tttctccaga cgctctctca tccatcgcct atgccaacca
      721 ggaaatttat ctcggcttga ttgtggcagg tgcggcaggg ttatcactat ccttgccgat
      781 cggcctcacc attgccggat tattaatcat tgtcgcgatc tcctattatc agacaattca
      841 cggttaccct cagggaggcg gctcttatat cgttacgcgg gaaaatttag gaaggatgcc
      901 cagtctgata gcgggtgcag ccatcctggt tgactatctc ctaacagccg ctgtcagcat
      961 taccgcaggt gtggaagccc tggcatcggc ttttcctgtc ctgtggggac atcgcgtcct
     1021 ggtttctctg ggtatcctgc ttgtcatcac cctgataaac ctcaggggat tgaaagaagc
     1081 cggcacgttc atgaccatgc cggtctacct gtttttattc acttacctgc ctttgctggc
     1141 ctatggcgcg gtccgcctgt ttatagacgg tcccagagat atccaggctg ttgcgcctgc
     1201 tgccagccag cctttggctg cttttctcat cctgcgtgct tttgcatcag gctgcactgc
     1261 cttaaccggc attgaagcaa taagcgatgg tgtccctgca tttcgccctc cgcaagcaaa
     1321 aaatgctggc aaaactctgg cggtgatggc agtactcatg tttattctgt tcgtgggcag
     1381 catcagcctg atctacagcc tggcggttgt tgccgggcca caggaaacca ttttatcggc
     1441 gctcgctcga agattactgg gatcgggacc gttatacatc cttattcaag ccagcacgat
     1501 gctcattttg acagtcgctg caaatacgag ttacgcagat ttcccacggg tggcatcttt
     1561 tctggcccgg gatggattcc tcccgcgaca gctcgccaac ctgggagacc ggttggtatt
     1621 ctcgaacggc attctccttt tagctgctgg tacagccgca ctgattatcg cattccaggg
     1681 agatacccat tcattggtac ccctttttgc gataggggcc ttcctggctt tcacactttc
     1741 gcagagcagt atggtgatgc attggatgcg cgagcgaggt cctaactgga aaatcaaagc
     1801 ttttgccaat ggggctggag caattgcaac agggacaacc ttgctggtgg tagcagtcag
     1861 taaattccag caaggtgcct ggttgaccat tctggtgatc ccaacggcgg taattatatt
     1921 cttgagaatc cacgcccatt accagaccgt cgcggcacag ttaaccttgc gtggattacc
     1981 ccccagtctg aaacctcaag gcacaatgcg ggcagtaatc ccggtttcag gagtgcaccg
     2041 tgggatgatc gatgcggtgc tttttgctca aaccatagcc cggcaagtga caggggtata
     2101 tatcgaatta aatccggagg cgagcgcaaa tgtccagaaa atgtggcagc gctggttccc
     2161 tgatattcct ctggttgtgc tgccttcgcc ataccgctcc attgtggagc ctttgctaaa
     2221 atttcttgat gaaacagatc gcgagcataa tgatggacag atggcagtcg ttgtcctgcc
     2281 cgaattcgtc ccagctcgct ggtggcaatc cttactccac aaccagacct ctcgtttaat
     2341 taaaaatgcc ctcctgtata gccggcggga aaaaggatac caacgcgtca tcattgatgt
     2401 gccctaccat ctcaaacgct gaacagcccg ggctgaagat tttaatcagt ggaatactcc
     2461 gcagcaagct ggttttatgt taaactaagc gattgccatg caacgctgga tgattttcct
     2521 tgttttgttt tttctcctgc atacccgggc agctccggca tatgctcagg cgctcacccc
     2581 gactgcacct gcaggaacga atataccttc tccaacaatc ggggtgagtg ctccggttgc
     2641 cggccaggct ttgcaaggcg tcattgcaat caccggcaat agtgcaatcc ccgaattcag
     2701 ccgcgctgag ctgtcttttg cttatgcaac ggacacaacc ggcacctggt tcttaatcgc
     2761 cgaaagtcag acgcccgtgg aaaacggtac cctggggcag tgggatacca ccaccattac
     2821 ggatggggaa taccagctcc gccttgtcgt ctatctgaca ggtgggcaaa aacaaatcgc
     2881 aacgaataca ggcctgcgag tccggaacta tactgcagtt gagactgaga cacccacacc
     2941 gtcggccaca atgcggcctg gtaatacgcc agcgcccagc aacacaccta cgcctacgtc
     3001 tacgcctgtt ccgcccacgg gtacaccggt gcctccaaac ccggccatca tcggacctgg
     3061 cgacttactc aggaatatgg ggcgcggggc gctgggaata gtgggtatct tcagcttgat
     3121 cggtatctat cgcggcctgc acagtttgat ccaaaaaaga tcgaggcagt agattgaaac
     3181 ccagagacaa ttccatctcc aatattctgg agtcattatt tgataggtta aaaacggacc
     3241 ccaagccaca ggtggagcct gaaaattcgg cggggatacc ctatttaatc gttggcctgg
     3301 gcaatccagg ccgcgaatat cgcactacac gccacaatat gggctttatg cttctggatc
     3361 gtctcgctga gaggctgggg gtcacatttt cgcggctgga atcccaggcg ctggtagcaa
     3421 agaccgagca cgccggcagc cggatcattc tggcaaaacc tcaggcatac atgaacctat
     3481 ccgggcagcc ggttggggct ttactccact attataagat tccggtctca caactgctgg
     3541 ttgcctacga tgatgtagat ctcccccttg gaagcttacg catccgccac ggaggtggtt
     3601 ctgccgggca gaaggggatg gcgtcaatca tcgagcgcct tggcacgcaa gaatttcccc
     3661 gtttgcgatt ggggatcgat cgaccgccag gcagaatgca agctgctgcc tacgttttgc
     3721 aggaattttc cagcctgcag aagccggtcg taaccaccgc actggatcgc gccgcagatg
     3781 ccgttttgct cttcatcagc gacggtctgg acgccgccat gaaccgttat aatggaacag
     3841 gaaccccgaa tggctctcag tgatctgatt gagtccatcg ccccgcattt tgagcctgtt
     3901 ttcgcagatt tacaggccga acagaattta tccgccctgg gattgcgccg ggcggcgcgt
     3961 ttaccatttc tggctgccct gcatcgtgaa atgcagcgcc cgatcatatt ccttaccgac
     4021 cgcaccgatc gggctctcgc aatgcaggaa gagctcagcc tg
//
LOCUS       DATMXK010000581         3690 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_142822, whole genome shotgun sequence.
ACCESSION   DATMXK010000581 DATMXK010000000
VERSION     DATMXK010000581.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 3690)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 3690)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..3690
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_142822"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(24..1532)
                     /locus_tag="VN363_10590"
     CDS             complement(24..1532)
                     /locus_tag="VN363_10590"
                     /inference="COORDINATES: protein motif:HMM:NF024948.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF4129 domain-containing protein"
                     /protein_id="HWQ85006.1"
                     /translation="MNDQSVMASGIKRDKSEIWTGLAAVLLILMEASWLIPWYLGVVE
                     ISYVASAMRAFLVLGGNMLEAYLVTRLAENLHLKRNLQQFLLAGLFLINMALAARLLL
                     DPATPDHLAGLLNLDPGPVLVVLAGAWLWWRGISLAQGTISPQTAWNRFWLGIWMLII
                     YLLVITRVTRMQPNIIPFLIFLGAGLLALIVARVAYIGQYHGSKRNPFGRGWIAAISG
                     AVSISILTSALFASLLTGQFKPYLAQLSSALRWVVSGIVFLVSIPGLILAYILTPLFE
                     AVRDFMERATNSTEAAPGLVGTPIAAPEIAAKAEPISIHPWVVTGLFWALILIVTILV
                     FNRVRYYRRLSAAQDLDDPEALLEKDELWRRLRHSAQQQIGDAGNLIRQLWANRSGQA
                     AYIRRIYAELLDLMEGLDMPRPAGATPLEYLPSTQHLLPGVEADLDTITQAYVRVRYG
                     ELPESAGEITEVEHAWQRISAAGGRQKRLQSARKKPLLEEQPSSQVREKNIS"
     gene            complement(1534..2721)
                     /locus_tag="VN363_10595"
     CDS             complement(1534..2721)
                     /locus_tag="VN363_10595"
                     /inference="COORDINATES: protein motif:HMM:NF013998.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="DUF58 domain-containing protein"
                     /protein_id="HWQ85007.1"
                     /translation="MTPRYWWPLLIVLFLLGIYLQVYLLSAFALMLGTISLGARWWAR
                     HALHNISYTRRLYYRRAFPGEIIPLTIETENRKFLPLSWLFVKDPWPKAVGPEDENIL
                     ARTHLPETGLLINLFSLRWYERARRYYQLLYRKRGVYELGPARLESGDIFGIFSQTDE
                     NGPIDQVTVFPKPLSLAAMDLPAGDPFGDRRTRRRLHEDPNLSMGIRDYRPEDDFRRI
                     HWPATAHSGELQVKVYQPVTAQVMSVCLNVSTFQYYWQGVDPDLLERLVSASAGLVEK
                     ALQDGYRVGMISNGCLMHSDQPFRISPGRSPDQLGIMMSALAAVTPFVTAPFERLLLR
                     EVHKLPPGTLLLILTSIVSMELRETLLQLKRHSRPVSVLAYCAKPPLPIPGIRMHHIP
                     YEA"
     gene            complement(2723..>3690)
                     /locus_tag="VN363_10600"
     CDS             complement(2723..>3690)
                     /locus_tag="VN363_10600"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013560239.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="MoxR family ATPase"
                     /protein_id="HWQ85008.1"
                     /translation="GPNNSLSEVNMIAEVSRRIQENIQKVIVGKDEAIQLTIAAVLCE
                     GNILLEDVPGIGKTSLARALAASLGCSFRRIQFTPDLLPSDVTGLNWFNQKEQSFEFK
                     PGPIMSQVLLADEINRATPRTQSALLEAMQERQVTIDGVTRALPRPFIVIATQNPVEL
                     EGTFPLPEAQIDRFLLRVRIGYPEESEENAMIERFRISDPLTELKSVTNPEEILALQA
                     ERRQIRVEESLRGYIVRIARATRKNTEIELGASPRATLALYQAAQAWAAIHNREYVIP
                     DDIKSLAPAVLTHRMIIAPQAQLRGRRPEEVVAGIVEGVPVPVEG"
BASE COUNT          844 a         1005 c         1047 g          794 t
ORIGIN      
        1 gccaggtatt tggcggtgag atttcatgag atattctttt ccctgacctg actggagggt
       61 tgctcctcca gcaggggctt tttgcgagcc gattgcagcc gcttctgccg gccaccggcg
      121 gcggatattc tctgccaggc atgctcaacc tcggtaatct cgccggctga ttcaggcagc
      181 tccccatagc gcacgcggac atacgcctgg gtgatcgtat ccaggtcggc ctcaacaccc
      241 ggaagcaggt gttgggtgga tgggagatat tctaagggtg tcgctccagc tggacggggc
      301 atatccaatc cttccatcaa atccagcagc tccgcataga ttctccggat ataggctgcc
      361 tgtccggatc ggttcgccca gagctggcgg atgagatttc cggcatcacc gatttgctgc
      421 tgggcggaat ggcgtaatct acgccagagc tcatcttttt ccaacaacgc ctctggatcg
      481 tccagatcct gggcagcgct caatctgcgg taatagcgca ctcgattgaa aacaaggatc
      541 gttacgatca ggataagtgc ccaaaacagc ccggtgacga cccatggatg gatactgatt
      601 ggttcggcct tcgcagcaat ttcaggtgct gcgatcggag tgccaaccag cccgggggct
      661 gcttcagttg agttcgtcgc tcgctccatg aaatcccgca ccgcctcgaa cagcggtgta
      721 agaatataag ccaggatcaa tccggggatt gaaaccagaa agacgattcc tgatacgacc
      781 cagcgtaagg cgctgctgag ctgggccaga tatggcttga actgcccggt gagcaggctc
      841 gcaaataaag cagaggtgag gatgctgata cttacggcac cactgatcgc ggcaatccaa
      901 ccccgtccga atgggttccg ttttgagccg tgatattgcc cgatgtaggc aacccgagcg
      961 acgattaatg ccagcaaacc agcccccaaa aaaatcagga aaggaataat atttggctgc
     1021 atgcgggtga cccgcgtgat caccaataag taaatgatca acatccagat acccaaccag
     1081 aaccggttcc aggctgtctg tgggctgatc gtgccctgtg ccaggctgat gcctctccac
     1141 cacagccagg ctcctgccag gacaaccagg accggacccg ggtcgagatt taacaatcct
     1201 gccaggtgat ccggtgtcgc gggatccagc agcaagcgcg cggccagagc catgtttatc
     1261 agaaataagc cggccagaag gaactgttga aggttgcgct tcaggtgcag gttttctgcc
     1321 aggcgggtaa ccaggtaggc ttcaagcata ttccccccaa gcacaaggaa ggcacgcatc
     1381 gcgctggcga cataactgat ttcgaccaca cccaggtacc agggtatcag ccagcttgct
     1441 tccatgagga tcaagagtac ggcagccaga ccggtccaga tctcagattt atcccgtttt
     1501 attccggagg ccataacaga ctggtcgttc atgctaagcc tcgtatggaa tgtggtgcat
     1561 ccggatgcct ggaatgggca ggggcggctt cgcacaataa gccagaacgc tgaccggccg
     1621 gctgtggcgc ttaagctgca gcagtgtttc gcgcagctcc atacttacaa tgctggtcaa
     1681 tattagcagg agggttcctg ggggcagctt atgaacctca cgcagcaata aacgctcgaa
     1741 tggtgcggtc acaaatgggg taacagcagc cagggcgctc atcatgatac caagctggtc
     1801 tggagaacgg cctggagata tccgaaaagg ttggtctgaa tgcatgagac agccattcga
     1861 gatcatccca acccggtagc catcctgcag ggctttctct accaggccag ctgaggcgct
     1921 taccagccgt tctaataaat ccggatcgac accttgccag taatattgaa aggtggatac
     1981 attcaggcac accgacatca cctgggctgt aaccggctgg taaaccttca cctgtagttc
     2041 tcctgaatgg gcggtggcag gccagtgaat acggcgaaaa tcatcttcag gccggtaatc
     2101 gcgaatgccc atactcaggt tgggatcttc atgcagccgg cggcgagtcc tccggtcacc
     2161 aaagggatct ccagcgggta aatccatcgc ggcgagcgat aaaggctttg gaaaaactgt
     2221 aacctggtca atcgggccat tctcatccgt ctggctgaaa atgccaaata tatcgccact
     2281 ttccagccgg gcgggaccaa gctcgtaaac gccgcgtttg cgatacagca gctggtagta
     2341 gcggcgggcg cgttcgtacc agcgcaagct gaacaggttg atcagtaaac cagtttccgg
     2401 cagatgagtc cgcgccaata tattctcatc ctccggacca actgctttcg gccaggggtc
     2461 cttcacaaat aaccaggaaa gaggcaaaaa tttacggttt tcggtttcga tcgtcagggg
     2521 aataatttcc ccggggaacg cacgccggta gtaaagccgc cgcgtgtagg atatattgtg
     2581 aagcgcgtgg cgggcccacc agcgagcccc caggctgatc gtccccagca ttaaggcgaa
     2641 ggcagagagc aaatagacct gcaggtagat tcccagcagg aagaggacta tcaggagcgg
     2701 ccaccagtat cggggtgtca cgttaacctt ctacaggtac ggggacgcct tcgacgatcc
     2761 ctgcgactac ttcttccggc cggcggccgc gcaattgggc ttgcggagcg atgatcatcc
     2821 ggtgggtgag cacagccggg gccagggatt tgatatcatc cggaattacg tattcgcggt
     2881 tatgaatagc cgcccaggcc tgagcagcct gataaagggc tagagtggcg cgtgggcttg
     2941 cgccaagctc gatctcggtg ttcttccgtg tggcccgggc tatcctgacg atgtatccac
     3001 gcaggctctc ttcaacccgg atttgccggc gttcggcctg taacgccagg atttcctctg
     3061 gattggtaac tgacttgagt tcggtaagtg gatcgctgat ccggaagcgt tcaatcattg
     3121 cattttcttc agattcttct ggatacccga tccggacgcg cagcaagaaa cggtctattt
     3181 gggcttcggg gagggggaat gtgccttcta gctcaaccgg attttgtgtg gcaataacaa
     3241 taaacggacg gggaagcgct cgcgtcacac cgtcgatggt cacctggcgt tcttgcatcg
     3301 cttccagcaa ggcggattgg gtgcgcgggg tggcgcgatt gatctcatct gccagcagca
     3361 cctggctcat gatcggacca ggtttgaact caaacgattg ctctttttga ttgaaccaat
     3421 tgagtccggt cacatcggag ggcaggagat cgggagtgaa ctggatgcgg cggaagctgc
     3481 agcccaggga ggctgccagt gcacgcgcca ggctggtttt tccaattccc ggcacatctt
     3541 ccaggagaat attcccttca cacagcacag ccgctatagt gagctgaata gcctcatctt
     3601 tcccgacgat cactttctgg atattttctt gaatccgccg gctgacctct gcaatcatat
     3661 tgacctcgga tagcgagtta ttagggcccg
//
LOCUS       DATMXK010000582         5198 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_155240, whole genome shotgun sequence.
ACCESSION   DATMXK010000582 DATMXK010000000
VERSION     DATMXK010000582.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 5198)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 5198)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..5198
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_155240"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..741)
                     /locus_tag="VN363_10605"
     CDS             complement(<1..741)
                     /locus_tag="VN363_10605"
                     /inference="COORDINATES: protein motif:HMM:NF016300.4"
                     /note="The SIMPL domain is named for its presence in mouse
                     protein SIMPL (signalling molecule that associates with
                     mouse pelle-like kinase). Bacterial member BP26, from
                     Brucella, was shown to assemble into a channel-like
                     structure, while YggE from E. coli has been associated
                     with resistance to oxidative stress; Derived by automated
                     computational analysis using gene prediction method:
                     Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="SIMPL domain-containing protein"
                     /protein_id="HWQ85009.1"
                     /translation="MRNRMPLIFGLLLVAAIAIAWGGPSIVKAQDPTPTAQAPLRTIT
                     ANGTGQVFLTPDIAYITIGVRSEGKVASEVLGSNNESADKLVQALRAAGIAANDIRTS
                     NFSIYPQQQYNAQGLPSGEITYVVENSLYVTVRDLEAVGDILDEAVQAGANSIYGIQF
                     DVANKEQQMGAARKAAVDNARQVAEELADAAGVTLGDVQAVTTSSGGFPGPIYGMGGA
                     VAREAAASVPVQTGELAFSVDVTMVFAIR"
     gene            923..3403
                     /locus_tag="VN363_10610"
     CDS             923..3403
                     /locus_tag="VN363_10610"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558883.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016887 - ATP hydrolysis activity
                     [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="AAA family ATPase"
                     /protein_id="HWQ85010.1"
                     /translation="MMRFDRFTERAQEAAQRAAEIIQRYGHNQIDTEHILLALIEQPG
                     GVVPQILEVLKIDSAVLTERLDYILRTSPKANIFGGGGGGQIFITPRVKRIIDLANEE
                     ANRLKDEYISTEHIFLAILSERSTPAARLLEGAGVTRERVYDAIQQIRGGQRVTDPQA
                     ETRYRTLERYSRDLTQLARGGKLDPVIGRDTEILRVIQILSRRTKNNPVLIGEAGVGK
                     TAIVEGLAQKIASNDVPEILSGKRVMSLDLGAMIAGSRFRGEFEERLKAALEEVQRAS
                     GEIILFIDELHTVVGAGAAQGAMDASSMLKPALARGELQCVGATTLDEYHKYIEKDAA
                     LERRFAPVYVEEPNVEDTIRMLQGLRDRYEAHHKVRFSDQALVDAARLSSRYVTERRL
                     PDKAIDLIDEAAAKLRVALYSLPANLKSMKTEIDRMTAEEEQAGLERDYERAAQKKAE
                     RLRLESEFKEQRDLWEAEHQLDEVVDTRDIAEVVAQWTGIPVNQMMETESQKLLQMEE
                     RLHEHIIGQDEAIGAISDAIRRSRSGLKDPRRPIGSFIFIGPSGVGKTELAKALAEFL
                     FDDADAMVRIDMSEYREQHTVSRLFGAPPGYVGYEEGGQLTEAVRRRPYRVILFDEIE
                     KAHPEVWNALLQILDDGRLTDGQGRVVDFRNSVLIMTSNLGTEYVRKSGSLGFLSRTG
                     TTEEQQDHEKIEKALKSTFRPEFLNRIDEIITFSPLSREQVAEIVVLQMKEIQERLSE
                     KGLSVELKPEAREWLADQGYDPAFGARPLRRALQKFVESPLSIRLLSGEFSSGDTIIV
                     DVDPEKKELVFHPVPDEAVATQLEPAAP"
     gene            3605..4081
                     /locus_tag="VN363_10615"
     CDS             3605..4081
                     /locus_tag="VN363_10615"
                     /inference="COORDINATES: protein motif:HMM:NF012257.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003700 - DNA-binding transcription factor
                     activity [Evidence IEA];
                     GO_function: GO:0030552 - cAMP binding [Evidence IEA];
                     GO_process: GO:0006355 - regulation of DNA-templated
                     transcription [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="Crp/Fnr family transcriptional regulator"
                     /protein_id="HWQ85011.1"
                     /translation="MPAVAFDHLRLFEGFSPDQLDLIQPLFSVCEQHDGDILFQQGET
                     AGYLYFVVEGEAVVRYKPDDGPWLTVARICPEGVLGWSAALGSPLYTSSGICTEDSVL
                     LRISAENLRRLCEEHPHTGSLVLERLATMIAQRLRNTHSYVVALLEQGLGVTIHNI"
     gene            4107..4364
                     /locus_tag="VN363_10620"
     CDS             4107..4364
                     /locus_tag="VN363_10620"
                     /inference="COORDINATES: protein motif:HMM:NF013286.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="NifU family protein"
                     /protein_id="HWQ85012.1"
                     /translation="MDAINGFTTYSEREQISALIEKISAYIEQVHGGSVKMIDYDGKI
                     LKVRLGGACEGCPLSPSTIHGWVEGTVRQFFPDIEKVVDVP"
     gene            complement(4438..>5198)
                     /locus_tag="VN363_10625"
     CDS             complement(4438..>5198)
                     /locus_tag="VN363_10625"
                     /EC_number="2.3.2.2"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013558952.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=3
                     /transl_table=11
                     /product="gamma-glutamyltransferase"
                     /protein_id="HWQ85013.1"
                     /translation="HLMIEAMRLAFADASWYVADPAFSPAPLAEMLSKGYAAERRKRI
                     DMRRANPDPRRGTPVSASDTVYFSVVDGSGNACSFINSNYMGFGTGIVPQGWGFALQN
                     RGHNFSLQPEHPNVLAPGKRPYHTIIPALVTDSQTGELIASYGVMGGFMQPQGHVQVL
                     VGLVDDELDPQAALDRPRFCIQDGKLGGQVALEDGLPANLLVGLENMGHRVTVVSGMR
                     RSIFGRGQVILRDAQSEVLCAGSDPRADGCAMCV"
BASE COUNT         1178 a         1478 c         1443 g         1099 t
ORIGIN      
        1 ccggatggcg aataccatcg tcacatcaac actgaaggcg agctcgcctg tctgaaccgg
       61 gacgctggca gcggcttcac gagcgactgc gccgcccatg ccgtagatcg ggccgggaaa
      121 tccaccgctt gaggtggtca ctgcctggac atcacccagg gtgacacccg cagcgtcggc
      181 aagttcctca gcgacctggc gggcattgtc cacggcagcc ttgcgggctg cacccatctg
      241 ctgctctttg ttcgccacat cgaactggat cccgtagatc gaattggcgc ctgcctggac
      301 agcctcatcc agaatatcgc ccaccgcttc cagatcgcgc acggttacgt agaggctgtt
      361 ctcaaccaca taagtgatct cgccgctcgg cagaccctgg gcattgtact gctgttgagg
      421 atagatgctg aagttgctgg tgcggatatc attggcggca atcccggcgg cgcgcagcgc
      481 ctgaacgagc ttatctgccg attcattgtt acttcctaaa acctccgagg ccactttacc
      541 ttctgagcgc acaccaatgg tgatgtaagc aatgtccggg gtcaggaata cctgcccggt
      601 gccgttggcg gtaatcgtcc gcaggggggc ctgggccgtg ggagttgggt cttgagcttt
      661 cacgatcgag ggaccacccc aggcgatcgc gatcgcagcg accagcaata aaccaaatat
      721 gagaggcatg cggttacgca tattattttc tcctttgaat aaataggttc tacgtactgg
      781 gaagtagacg ccatcccccc aaaaatggtt cccggatggg tacaggtttc atatacaatt
      841 cttatatgcc tcctgcactg cattgataac cgcgatgtta taatgtcgcc atcagccctg
      901 accggcagaa aaggaaacta ttatgatgcg atttgatcga tttactgagc gagctcaaga
      961 agccgctcaa cgtgctgccg agattatcca gcgttatgga cacaaccaga ttgacaccga
     1021 gcatatcctg ctggcgctga tcgaacagcc ggggggagtt gtcccccaaa ttttagaggt
     1081 tttaaaaatt gactctgcgg tgctcactga gcgcctggat tatattctcc gcaccagccc
     1141 caaggccaat atttttggcg gcggcggtgg tggacagatt ttcattaccc cgcgggttaa
     1201 acggattatt gatctggcaa atgaagaagc caaccgcctg aaggatgaat acatctccac
     1261 cgagcatatc ttcctggcaa tcctgagcga gcgcagcacc cctgcggcgc gcctcctgga
     1321 aggcgccggt gtgactcgtg agcgggtcta cgacgcaatc cagcaaattc gcggcggtca
     1381 gcgcgtgact gacccgcagg ccgaaactcg ctaccgcacc ctggagcgtt attcccgcga
     1441 cctcacccag ctggcccgcg gcggcaagct ggaccccgtc atcggacgcg ataccgagat
     1501 tttgcgcgtg atccagatct tatcccgccg caccaagaac aacccggtct taatcggtga
     1561 agctggagtg ggtaagactg ccattgtcga aggcctggct cagaaaattg ccagcaatga
     1621 tgtccccgag atcttatccg gcaagcgggt catgtcgctc gacctgggcg ctatgatagc
     1681 cggctcgcgc tttcggggtg aatttgaaga acgtttgaaa gctgccttag aggaagtcca
     1741 gcgtgcctct ggagagatca ttctcttcat cgacgagctg catacggtcg ttggcgcggg
     1801 agcagcccag ggtgcaatgg atgcctccag catgttgaag cccgccctgg cccgcggcga
     1861 gctgcagtgt gtcggtgcta cgacgcttga cgagtaccac aaatatatcg agaaagatgc
     1921 tgctctcgag cgccgttttg caccggtgta tgtcgaagag cccaatgtgg aggataccat
     1981 ccgtatgctt cagggtctgc gcgaccgcta cgaagctcac cacaaggtcc gcttctccga
     2041 ccaggccctg gtggatgccg cccgtctttc ctcccgctac gtaaccgagc gccgcctgcc
     2101 ggataaagcc atcgacttga tcgacgaagc tgccgccaag ctgcgcgtgg ccctgtattc
     2161 actgcccgca aatcttaagt ccatgaagac cgaaatcgat cgcatgacgg cggaagaaga
     2221 gcaggctgga ttggagcgtg attacgagcg tgcggctcag aaaaaagccg agcgattgcg
     2281 tctggaaagt gaattcaaag agcagcgcga tctttgggaa gccgaacacc agctggatga
     2341 ggtcgtcgac actcgcgata tcgccgaggt cgtcgcccag tggacgggaa tcccggtcaa
     2401 ccagatgatg gagaccgaat cccagaaact tctgcagatg gaagagcgcc tgcacgaaca
     2461 tatcatcggg caggatgagg ctattggcgc gatctctgac gcaattcgcc gctcgcgttc
     2521 gggtcttaag gaccctcgcc gcccgattgg ctcatttatc tttatcggcc cctccggggt
     2581 cggcaaaacc gaactggcca aagctttagc tgaattcttg ttcgatgacg cagacgccat
     2641 ggtgcgcatc gatatgagcg aataccgcga gcagcatacc gtctcacgcc tgtttggagc
     2701 acctcctggc tacgttggct acgaagaagg tggccagctc accgaggcgg tccgccgccg
     2761 gccttaccgg gtaatcctgt tcgacgagat cgaaaaagcc catcccgaag tttggaacgc
     2821 gttgctccag atcctggacg atggacggct gacagatgga cagggaaggg tggttgattt
     2881 ccgcaattct gtcctgatca tgaccagcaa cctgggcacc gaatacgtgc gcaagtccgg
     2941 cagtctggga ttcctgtctc gcactggcac gaccgaagag cagcaagacc acgaaaagat
     3001 cgagaaggct ttaaagagca ccttccggcc cgagttcctg aaccgcatcg acgagatcat
     3061 taccttctcg ccgttatccc gggagcaggt agcagagatc gtcgtcttgc agatgaagga
     3121 aatccaggaa cgtctctccg agaagggtct ttcggttgaa ttaaaaccgg aagcccgcga
     3181 gtggctggcg gaccagggat atgatcctgc gtttggtgcc cgcccattgc ggcgagcgct
     3241 gcaaaagttt gtggaaagcc cgctttctat ccgcctgctc agtggagaat tcagctccgg
     3301 ggatacgatc attgtcgatg tcgatcccga gaagaaggag ctggtcttcc accccgtccc
     3361 ggacgaggct gtggccacgc agctggagcc ggctgcccca taatttaatt ccgatcgctg
     3421 aacggaccgc tgcgaaaatc agcggtccgt tttgattcac agggtcataa cgccagggac
     3481 tattttcaca agttcgacgg gacaaagctc cagtgcggga tgatatttgt ctatgcaatg
     3541 aggatatttg ccactatctc aagaaacgat ttgtggagta aattgaaggt acggaccatc
     3601 cattatgccg gcagttgcat tcgatcacct ccgcctcttt gaaggcttta gccccgacca
     3661 gctagacttg atccagccgt tgttctctgt ctgtgaacaa catgatggag atatcttgtt
     3721 tcagcaaggc gaaaccgccg gatacttata ctttgtagta gaaggcgaag ctgtggtacg
     3781 atataagccg gatgatggtc cctggctaac cgtagcgcgc atctgtccgg aaggggtgtt
     3841 aggttggtcg gcagccttgg gcagccctct ctatacatcc tccgggatat gcaccgagga
     3901 cagcgtttta ttgcgtatca gcgcagagaa tctgcgtcgc ctgtgtgagg agcaccctca
     3961 taccggaagt cttgtcctgg aacgcctggc aaccatgatt gcccagcgct tgcgcaacac
     4021 ccacagctac gtagtagccc tgctggaaca gggtctgggt gtcaccatcc acaacatata
     4081 atccgcctgg ctggaggaag ttaccaatgg atgcaattaa cggttttacc acctattcag
     4141 aacgcgaaca gatcagcgcc ctgatcgaaa aaattagcgc ctatattgag caggtccatg
     4201 gtggatcggt caagatgatt gactatgacg gaaagatctt aaaagtccgc ctgggcggtg
     4261 cctgcgaagg ctgcccgctt tcccccagca ccattcatgg ttgggtagaa ggcactgtgc
     4321 gccagttctt ccccgatatc gaaaaagtgg ttgacgtacc ctgattgaaa tcagtggatg
     4381 attaatcaat aaaaggggat ccacatattt ctggataccc tttttgattc tcgcaggtta
     4441 cacacacatc gcgcagccat cggcccgggg atcagagccg gcacacagca cctcggactg
     4501 ggcatcccgc aggatcacct gcccgcgccc gaaaatcgag cggcgcattc cactcactac
     4561 cgtcacccgg tgacccatat tttcaagccc caccaacaga ttggctggca agccatcctc
     4621 gagtgcaacc tgacctccca actttccatc ctggatgcaa aagcgcggcc tatccaaagc
     4681 cgcctgggga tccagctcat cgtctaccag gcctaccagc acctgcacat gcccctgcgg
     4741 ctgcataaaa ccacccatca caccataaga ggcgattaac tccccggtct ggctgtcggt
     4801 gaccagggca ggaatgatgg tgtgataagg gcgtttgcct ggcgccagca cattaggatg
     4861 ctccggctgc aggctgaaat tgtggccgcg attttgcaag gcaaaacccc atccctgggg
     4921 gacgatcccg gttccaaaac ccatgtaatt gctgttgata aatgagcagg cattcccgga
     4981 gccgtcgacc acactgaaat agaccgtatc cgaggcgctg accggtgttc cccgcctggg
     5041 atccgggtta gcgcggcgca tatcaatccg cttgcggcgc tcggcagcat agcctttact
     5101 caacatttcg gcgagaggtg ccgggctgaa agccgggtcg gccacgtacc agctggcatc
     5161 ggcaaatgcc aggcgcatgg cttcgatcat caggtggt
//
LOCUS       DATMXK010000583         2851 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_72519, whole genome shotgun sequence.
ACCESSION   DATMXK010000583 DATMXK010000000
VERSION     DATMXK010000583.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2851)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2851)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2851
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_72519"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            <1..412
                     /locus_tag="VN363_10630"
     CDS             <1..412
                     /locus_tag="VN363_10630"
                     /inference="COORDINATES: protein motif:HMM:NF024490.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=2
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ85014.1"
                     /translation="SQAGVQVDLIVRGMCCLRPGVPGISENIRVISVVGRFLEHSRVY
                     YFENGGKEQIFLGSADLMPRNINRRVEVLFPVSEQRLIRYLRDDVLESYLKDNVKARL
                     MKKDGTFIRLKPKGNDPESNVQEWLLQFNQTSQK"
     gene            445..2334
                     /locus_tag="VN363_10635"
     CDS             445..2334
                     /locus_tag="VN363_10635"
                     /inference="COORDINATES: protein motif:HMM:NF017085.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="CHAD domain-containing protein"
                     /protein_id="HWQ85015.1"
                     /translation="MTLTAEERSALEALLKAGPSASHQRRAEVVLLTDQGLTSREIAG
                     KTGLKPTGVRYWRAQFRMRGMGIFGEVEAKQEAMQEAVQQAVQEASPIALPEEAEPRV
                     EEPTAADDAAPEANPASGKVTGGNSQAYVRDLVLDLFDRTQPWHGLSAADRYLLGEAV
                     SHSKANLPKAARRDLEAARAWLETLGYAQLNPDEQNKLAGLILMQRGKIKTGELQRLG
                     LSESLHKELVTMLGLLRMGWGLDASHRQQTHIEAIKKIRGGLRVVVSGATASQDAAAA
                     QQSAGMWTWVGLPRVEVTDKVSAQAHHPADLPLPLPLERTNLSADDSLGEAGRKVFLF
                     HFAQMLAHEPGTRSGEDIEELHDMRVATRRMRAAFEVFGDAYEPKVIKNLLKGLRATG
                     RALGHPRDMDVFMEKAHLYLNGLPEDQHASLDPLLAAWEQERQSARQEMTAYLDSSDY
                     AEFVERFNRFAQTPGSGALPVPAEQPAPKRLREVVPVLIYSRIASVRAFEPILSNARI
                     EQLHALRIEFKKLRYAVEYFREVLGPEASKVIEVIKRLQDHLGELNDANVATQLLRSF
                     LDDWDGSQESLPISERQNPGGIIAYLAYQHARRHELMITFQQAWTEFASPEFRQMLAA
                     AIAVL"
     gene            complement(2331..>2851)
                     /locus_tag="VN363_10640"
     CDS             complement(2331..>2851)
                     /locus_tag="VN363_10640"
                     /inference="COORDINATES: protein motif:HMM:NF012383.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_process: GO:0009116 - nucleoside metabolic process
                     [Evidence IEA]"
                     /codon_start=3
                     /transl_table=11
                     /product="phosphoribosyltransferase family protein"
                     /protein_id="HWQ85016.1"
                     /translation="GGMQNRRELLTWKDVDKLIDHLLPQFEGEFDAMVMITRGGIIPG
                     GMLAEAIGLTHILTAAVDFPAQTEMEKTKLMAWPQFIQFPEDSLVRGRRTIVVDDVWG
                     SGRTITTVRHRVSSAGGIPSTCVLHFNPYRNLFNTTKPDYYAAVTDAHIVYPWEINRG
                     PDMVLMGEGRTG"
BASE COUNT          640 a          747 c          873 g          591 t
ORIGIN      
        1 ctcgcaggct ggtgttcagg ttgacctgat cgtacgggga atgtgctgct tgcgtcccgg
       61 tgtgccgggg atcagcgaga atatccgcgt gatcagtgtg gtgggacgct tcctggaaca
      121 cagccgggtg tactattttg aaaatggtgg gaaggaacaa atttttctgg gcagcgctga
      181 cctgatgccg cgcaatatta accggcgggt agaagtcctg ttccctgtct ctgaacagcg
      241 cttaattcgc tatttacggg atgatgtcct ggaatcgtac ctcaaagata atgtgaaggc
      301 acgcctgatg aaaaaggatg gtacttttat ccggctgaag ccgaaaggca atgacccgga
      361 gagcaatgtt caggagtggc tgctccagtt caaccagact tctcagaaat aaatagtgag
      421 ttttttgaaa gggatctggc gactatgact ctaaccgcag aagagcgaag tgcactggaa
      481 gcgttattga aagccgggcc gtccgcaagc catcagaggc gggcagaggt ggtgctgctt
      541 accgaccagg gtctcaccag ccgtgagatc gccgggaaga ccgggttaaa gcccactggg
      601 gtccgctact ggcgggcaca attccggatg cgcggcatgg ggatttttgg tgaagtggag
      661 gccaaacaag aggcgatgca agaagcagtg cagcaagcag tgcaggaagc gtcgcccatt
      721 gcgttgccag aagaagctga accacgggtt gaagaaccca ctgcagctga tgatgctgct
      781 ccggaggcga acccggcctc aggcaaagtc accgggggga acagtcaggc atacgtgcgt
      841 gatctggttt tggatttgtt cgatcgtacc cagccctggc atggattgag cgcggccgac
      901 cggtatttgc tgggggaagc ggtgagccat tcgaaagcga atctccctaa agctgctcgc
      961 agggacctgg aggctgcgcg agcgtggctg gaaacattgg gttacgcgca gcttaacccg
     1021 gacgagcaga ataagctggc tgggcttatc ctcatgcagc gcggcaaaat caaaaccggg
     1081 gagctccagc gcctgggctt atccgaatcc ctgcacaaag agttggtgac gatgctggga
     1141 ctgctgcgga tgggctgggg actggacgca tcccaccgcc agcaaaccca tatcgaggcg
     1201 atcaaaaaga tccgtggcgg cttgcgggtg gtggtgagcg gggcaactgc aagtcaggac
     1261 gcggctgcag cccagcaaag cgcggggatg tggacgtggg tgggcctgcc gcgggtcgaa
     1321 gtgaccgata aagtcagcgc tcaggcgcac caccccgctg acctgccgct gcctttgcct
     1381 ctggagcgca ccaacctctc agcggacgat tcattggggg aggcgggacg aaaagtcttt
     1441 ctgtttcact ttgctcaaat gctggctcac gagccaggca cccgttcagg cgaggatatc
     1501 gaagagctgc atgacatgcg cgtggctacc cggcggatgc gggcagcctt tgaggttttt
     1561 ggggatgcct acgagccaaa agttatcaag aatttgctca aaggtttgcg agccaccggg
     1621 cgggcgctgg gccatccacg ggatatggat gtgttcatgg agaaggccca tctttattta
     1681 aacggcctgc ctgaagacca acacgccagc ctcgacccgc tgctggcagc ctgggaacag
     1741 gaacgccagt cggctcggca ggaaatgacc gcttatctcg atagctcgga ttacgccgag
     1801 tttgtcgagc gcttcaaccg gtttgcgcaa accccaggat ccggtgcact gccggtacca
     1861 gccgaacaac cggccccaaa acgcttgcga gaggtggtac cggtcttgat ctacagccgg
     1921 atagcatcgg tgcgcgcctt tgagcctatc ctgagcaatg cccggataga gcagcttcac
     1981 gccctgcgga ttgaatttaa aaaactacgc tatgcggtgg aatattttcg agaagttctt
     2041 ggccccgaag cgagcaaggt aattgaggtc attaaaaggc ttcaggatca cctgggtgaa
     2101 ttgaacgacg ccaatgttgc cacgcagctc ctgcggagct tcctggatga ttgggatggc
     2161 agccaggagt ccttacccat ctctgagcgc cagaatccgg gtggcatcat cgcctacctg
     2221 gcttaccagc atgcccggcg gcatgagtta atgatcactt tccagcaagc ctggacagaa
     2281 tttgccagcc ctgagttccg ccagatgctg gcggcggcga ttgccgtgct ttaacctgtt
     2341 cggccttcgc ccatcaggac catatctggt ccgcgattga tttcccaggg ataaacgata
     2401 tgggcgtcgg taactgcggc gtagtaatca ggtttggtag tgttgaacag gttgcggtaa
     2461 gggttaaagt gcagcacaca ggtggaagga attccgccgg cacttgaaac gcggtgcctg
     2521 acagtggtaa tcgtccgccc cgagccccag acatcatcga caacaatcgt cctgcggcct
     2581 cgcaccaggc tgtcctctgg gaattggata aattgaggcc aggccatcag cttggtcttt
     2641 tccatttcgg tctgggcggg aaaatcgacg gctgcagtca agatatgggt gagaccgata
     2701 gcctcagcca gcattcctcc ggggatgatt cccccgcggg tgatcatcac catggcatcg
     2761 aattcacctt caaactgcgg cagcagatgg tcgatcagct tatccacatc tttccaggtc
     2821 aataattctc ggcgattttg catgccgccc t
//
LOCUS       DATMXK010000584         2853 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_27698, whole genome shotgun sequence.
ACCESSION   DATMXK010000584 DATMXK010000000
VERSION     DATMXK010000584.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2853)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2853)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2853
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_27698"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            complement(<1..325)
                     /gene="rpsI"
                     /locus_tag="VN363_10645"
     CDS             complement(<1..325)
                     /gene="rpsI"
                     /locus_tag="VN363_10645"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559460.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005840 - ribosome [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="30S ribosomal protein S9"
                     /protein_id="HWQ85017.1"
                     /translation="MAVQYYEGIGRRKESTARVRVMAGSGKFVVNDKPGPEYFTRDGD
                     VNLIMAPILAAGSAPEQLDVSVVVQGGGVTGQTSAVQLGVARALVKMNPDWQPLMRKG
                     GFLTRD"
     gene            complement(338..775)
                     /gene="rplM"
                     /locus_tag="VN363_10650"
     CDS             complement(338..775)
                     /gene="rplM"
                     /locus_tag="VN363_10650"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559459.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0000315 - organellar large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L13"
                     /protein_id="HWQ85018.1"
                     /translation="MSVEKTYIPKAAEISQQWYLVDANDQILGRVATQIASILLGKNK
                     PNYTPGMDTGDYVIVVNCERVRVTGKRLDDKIYYHHTQYPSGIKSVDLRTQLRRHPDR
                     VIRAAVWGMLPKNKFGRQLIKKLKIYAGPEHPHGAQQPSVVKA"
     gene            complement(831..1583)
                     /gene="truA"
                     /locus_tag="VN363_10655"
     CDS             complement(831..1583)
                     /gene="truA"
                     /locus_tag="VN363_10655"
                     /EC_number="5.4.99.12"
                     /inference="COORDINATES: protein motif:HMM:TIGR00071.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0009982 - pseudouridine synthase activity
                     [Evidence IEA];
                     GO_process: GO:0006400 - tRNA modification [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="tRNA pseudouridine(38-40) synthase TruA"
                     /protein_id="HWQ85019.1"
                     /translation="MARYQVNLVYDGTLFFGFQRQAKQRTVQSAVEDALHQLGWQGVS
                     LLAAGRTDTGVHASGQVIAFDLDWNHSEQALLNALNACLPSDVAARQVTTAEDDFHPR
                     YAARARWYRYRLYLSAVRDPLRDRFAWQVWPVPDQSRLDILANLLVGRHDFGAFGTPP
                     KAGRSTQRQIFRASWQGNGDELCFDVTGNAFLYRMVRRLVGLQVRTAQNRLPMEVISS
                     YLERPDASRVQELAPPQGLTLMKVYYSLEEML"
     gene            complement(1632..2009)
                     /gene="rplQ"
                     /locus_tag="VN363_10660"
     CDS             complement(1632..2009)
                     /gene="rplQ"
                     /locus_tag="VN363_10660"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559457.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_component: GO:0005762 - mitochondrial large ribosomal
                     subunit [Evidence IEA];
                     GO_component: GO:0022625 - cytosolic large ribosomal
                     subunit [Evidence IEA];
                     GO_function: GO:0003735 - structural constituent of
                     ribosome [Evidence IEA];
                     GO_process: GO:0006412 - translation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="50S ribosomal protein L17"
                     /protein_id="HWQ85020.1"
                     /translation="MRHRVAGFKLSRSKDQRIALRRNLVKQLFEHERIRTTRTKALAV
                     RGQAERLITLAKTGNKAGDAQMVHARRLAAARLADPAAVRKLFDDIAPRYETRPGGYT
                     RIIKLGPRSGDAAEMVLLELVEG"
     gene            complement(2016..>2853)
                     /locus_tag="VN363_10665"
     CDS             complement(2016..>2853)
                     /locus_tag="VN363_10665"
                     /EC_number="2.7.7.6"
                     /inference="COORDINATES: similar to AA
                     sequence:RefSeq:WP_013559456.1"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0003677 - DNA binding [Evidence IEA];
                     GO_function: GO:0003899 - DNA-directed 5'-3' RNA
                     polymerase activity [Evidence IEA];
                     GO_function: GO:0046983 - protein dimerization activity
                     [Evidence IEA];
                     GO_process: GO:0006351 - DNA-templated transcription
                     [Evidence IEA]"
                     /codon_start=2
                     /transl_table=11
                     /product="DNA-directed RNA polymerase subunit alpha"
                     /protein_id="HWQ85021.1"
                     /translation="LSSLEGAAVTSIRIADVQHEFSDIPGVREDVIRVMLQIKLLRMK
                     LYDVDSTRMHLEVRGSGVVTAADIIAPPEVEIINPDLFLFTVDDKNAHLEIEMTVERG
                     RGYSPADDRTGRLPIGELPVDAIYGPVRRVNWDIGYARVGQSTNYDRLMMEIWTDGTV
                     GPEQALSTSAKILIDHFRHLAGISEETLAAITTEEEETSRLTSEMIETPIENLDLSVR
                     VFNSLKRTGITTVGEVLELLEKGDEAVMSIRNFGEKSLDELRQKMREKGYLQDGNENE
                     AE"
BASE COUNT          627 a          857 c          702 g          667 t
ORIGIN      
        1 aatctcgggt caagaagccg cctttacgca tcaaaggctg ccagtcggga ttcatcttga
       61 ccaacgctcg cgcaaccccc agctgaacag cggaagtctg tccggtgacg ccaccgccct
      121 gaaccacaac cgacacatcc agctgctcag gagcactgcc ggctgccaga atgggagcca
      181 taatcaggtt gacatcgcca tctcgagtga aatattcggg acccggctta tcgttgacta
      241 caaacttacc ggaaccagcc atcacccgaa cacgggcagt gctttcctta cggcgtccga
      301 taccctcata atactgtact gccataattc ctactcctta cgccttcacc acgctgggct
      361 gctgtgcgcc atgggggtgt tcggggccag cataaatctt cagcttttta atcagctggc
      421 ggccaaattt atttttcggc agcatgcccc aaactgctgc ccggattacg cgatctggat
      481 gccgtcgcag ctgagtgcgc agatctacag atttgatccc gctgggatac tgggtgtgat
      541 gatagtaaat cttgtcatcc agtcgcttgc cggtaacgcg cacgcgctcg cagttcacca
      601 cgatcacata atctccggta tccatgcccg gcgtataatt cggcttattt ttgcccagca
      661 atatgctggc gatttgggtg gcgacccgac ctaagatctg gtcattggca tccaccagat
      721 accactgctg ggaaatctct gccgcttttg gtatgtatgt cttttccacg ctcatcatct
      781 ccacgattac gagataacca actcgtttac gtctaacaaa ttgatcctgc ctacaacatt
      841 tcttccaagg agtagtacac cttcattaag gtcaaccctt gtggaggtgc cagctcctgc
      901 acccggcttg catccggtcg ctccaggtaa ctgcttataa cttccatagg aagcctgttc
      961 tgagcagtcc gcacctgcaa gcctaccaga cggcgcacca tgcgatacag gaatgcgttc
     1021 ccggttacat caaagcacag ctcatcgccg tttccttgcc agctggcccg aaagatctgc
     1081 cgttgggtgc tccgcccggc tttgggtgga gtaccaaacg caccaaaatc atgcctgcca
     1141 accagaagat tagcaaggat atctaaacgg ctttgatccg gaactggcca gacctgccag
     1201 gcaaaacgat ccctgagagg gtctcgtaca gccgagaggt acaaccggta gcggtaccag
     1261 cgagctcggg ctgcatagcg gggatgaaaa tcatcctcgg ctgttgtcac ctgtcgggca
     1321 gccacatccg atggcaagca tgcatttagc gcattcagca gtgcctgctc cgagtgattc
     1381 caatccagat cgaaagcaat cacctgtccg gaagcatgca caccggtatc agtccgcccg
     1441 gcggcaagga gggacactcc ctgccagccc agctggtgaa gagcatcttc tactgcgctc
     1501 tgcaccgtgc gctgcttcgc ctgtcgctgg aaaccgaaaa acagcgttcc gtcgtagacg
     1561 agattaacct ggtaacgtgc catttctagc gcagcgacca gccagggtgt aagcctggca
     1621 acagctgcgt tctaaccttc aaccagctct aagagcacca tttccgctgc atcgccgcta
     1681 cgtggtccaa gcttgataat gcgggtatat ccgccaggcc gggtttcgta acgcggggca
     1741 atatcatcga agagcttgcg cacagcagcc ggatcagcaa ggcgggcggc tgccaaccgg
     1801 cgagcatgca ccatctgtgc gtcgcctgcc ttgttgccag tctttgccag cgtgattaaa
     1861 cgctcggcct gcccacgcac tgccagtgcc ttggtccgag tagtacggat ccgctcgtgc
     1921 tcaaataact gcttcaccag attgcggcgt aaggcaatcc gttgatcttt actgcgactc
     1981 aatttaaatc cagctactct atgtcgcatt tctcattact ccgcttcatt ctcattacca
     2041 tcctggaggt accctttttc gcgcatcttc tgacgcagtt catccaggct tttttcacca
     2101 aagttgcgga ttgacatcac cgcttcgtcg cctttttcga gaagctccaa cacctcaccc
     2161 accgtggtga tcccggtgcg cttgagtgaa ttaaacacgc gcactgaaag atccaggttt
     2221 tcgatcggtg tttcgatcat ctcgcttgtc aggcggctgg tttcttcttc ttcggttgta
     2281 atcgctgcca gagtctcttc actgatcccc gccagatggc ggaagtgatc gatcaagatc
     2341 ttggcgcttg tgctgagagc ttgttccggc ccaacggttc cgtcagtcca gatttccatc
     2401 atcaatcggt cataattcgt gctctgccca acccgggcat agccaatatc ccagtttacc
     2461 cggcgcacag gaccgtagat tgcgtctacg ggaagctcgc caattggcaa acggccggtg
     2521 cgatcatctg cgggcgagta acctcgaccg cgctcgactg tcatttcgat ctccaggtga
     2581 gcatttttgt cgtccaccgt aaagagaaac aagtcgggat tgatgatctc cacttcggga
     2641 ggagcgatga tatcggctgc cgtaactacc ccactcccac gcacctcgag gtgcatgcgg
     2701 gtgctgtcca cgtcgtataa cttcattcgc agcagcttga tttggagcat aacccggatc
     2761 acatcttcac gcacgcccgg gatgtcgcta aactcatgct gcacatcggc aatacgaatc
     2821 gaggtgaccg ccgcgccttc aagagaggat aat
//
LOCUS       DATMXK010000585         2273 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_76723, whole genome shotgun sequence.
ACCESSION   DATMXK010000585 DATMXK010000000
VERSION     DATMXK010000585.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2273)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2273)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2273
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_76723"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            188..583
                     /locus_tag="VN363_10670"
     CDS             188..583
                     /locus_tag="VN363_10670"
                     /inference="COORDINATES: protein motif:HMM:NF024864.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology."
                     /codon_start=1
                     /transl_table=11
                     /product="cupredoxin domain-containing protein"
                     /protein_id="HWQ85022.1"
                     /translation="MFRKLIVGFALLASVLALAGCASGPEKISVTTTEFKFEPLTWEV
                     SAGKEVELTLINTGTLEHEWVIVNQGQEVTIPFDDDDEAKVYWEMEAMAGETKVGTFT
                     APSEPGTYTIVCGTPAHLEAGMTGTLTVK"
     gene            875..1477
                     /locus_tag="VN363_10675"
     CDS             875..1477
                     /locus_tag="VN363_10675"
                     /inference="COORDINATES: ab initio
                     prediction:GeneMarkS-2+"
                     /note="Derived by automated computational analysis using
                     gene prediction method: GeneMarkS-2+."
                     /codon_start=1
                     /transl_table=11
                     /product="hypothetical protein"
                     /protein_id="HWQ85023.1"
                     /translation="MSTASTKPTAPLAILIQWLLFTAFGCWSGLMLGSWLTPRIANTL
                     NGNEDSMFPYIVLLALGVTLGVVQSVALSRVLPAVKGWLRYTLMGFVGAMVVVAIASS
                     LGLGSAAGLLDDALLFALLGAVIGLGQWRLLRQAFPQAWLWILATALGGLIFLQLAVT
                     PAAETNEFVVRGTLYGLIAALPAGLALAWLSRQFGSLARK"
     gene            1629..2129
                     /locus_tag="VN363_10680"
     CDS             1629..2129
                     /locus_tag="VN363_10680"
                     /inference="COORDINATES: protein motif:HMM:NF012436.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0008199 - ferric iron binding [Evidence
                     IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="ferritin-like domain-containing protein"
                     /protein_id="HWQ85024.1"
                     /translation="MGTKGRQIVGMDVEVLLSMLGEAFADEWLAYYQYWLGAKIVKGP
                     MKDAVAAELLLHATEELAHADLLALRIIQLGGTPPISPAAWLATTHCGYAAPTDPFVK
                     QILAQNIAGEQCAIDVYQNLLKIVREPDPVTYNLILTILQQEVEHEEDLQALDEDLES
                     LMMRSL"
BASE COUNT          464 a          688 c          617 g          504 t
ORIGIN      
        1 gtgctataat atagacgatt cagacaaaca ataccgtaat actttcgggg gggaaccaac
       61 ctcgaaagcc atccgctacc cggtaaaaac cacaatttca tcgtccccat ccctggttgt
      121 gattgcttgg gctggtccga atcgcaagcc gtggtcgtcc ttttattcta acctgaggag
      181 ggtccgcatg tttcgaaaat tgatcgttgg gtttgctttg ctggccagtg ttctggccct
      241 ggcggggtgc gcttccggcc ccgagaagat ctccgtcacc accaccgagt tcaaatttga
      301 gccgctcacc tgggaagtct ctgccggtaa ggaggtcgag ctgaccctga ttaatacagg
      361 caccctggag cacgagtggg tgattgtcaa tcagggccag gaagtcacta ttcccttcga
      421 cgatgatgat gaagcgaagg tttactggga gatggaagcc atggcgggcg aaaccaaggt
      481 tggcaccttc accgcgccca gcgagcccgg cacgtacacc atcgtttgcg gcaccccggc
      541 gcacctggag gccggtatga ccggtacgct gaccgtcaag taagctgtac tcagcttagc
      601 cctgcccggg aagtacaccc atcaaatagg cgcaaagcct gcacaggctc tgcgcctgtt
      661 ttggttaatc gatcatccaa atccgctgcc aggtgttgta caattgaatc aacaggcgag
      721 ccgccgggcg cgggatccgc tttggcttcc ttgcctcccg cgcccaggca tctctccatc
      781 ctccggcctc acccccgcag agttgatgta caatagctcc aatcggcgcc gcgcggctgc
      841 ctgatccacc caccttgcct gcggaggaaa tttcatgtcc acagcctcaa ccaaaccaac
      901 cgctcccctg gccatcctca tccaatggct gctgttcacc gccttcggct gttggtccgg
      961 cctcatgctg ggcagctggc tcaccccccg gattgcaaac actctcaatg ggaatgaaga
     1021 cagcatgttc ccctacatcg tcctgcttgc gctgggagtt accctgggtg tggtgcagtc
     1081 cgtagcgctg agcagagtgc tgccggcggt gaaaggctgg ctgcgctaca ccctgatggg
     1141 tttcgtgggg gcgatggtgg tcgtggctat tgccagctca ctcgggttgg ggtccgccgc
     1201 cggcctgctg gacgatgccc tgctgtttgc tctgctgggc gcggtcatcg ggctgggcca
     1261 gtggaggctg ctgcgccagg ctttcccgca ggcctggctg tggatcctgg caaccgccct
     1321 gggtgggctg atcttcctgc agctggcagt cacccctgcc gccgagacca acgagtttgt
     1381 ggtgcgcggc accttatatg ggctgatagc cgccctgcct gccgggctgg ctctggcctg
     1441 gctcagccgg cagtttggat cgcttgctcg taaatgagtg ttaaccctgt tgatttaagt
     1501 cactcatctg ccagaatatc agttttactc acccggtgcc cacatcgata atagagcgaa
     1561 agtcgggtat atttgttcgt gataaaattt acaagtaaat cataatcaac aatcgaaagg
     1621 aaaataaaat gggaaccaaa ggacgtcaaa tcgttggcat ggatgtggaa gtcctgctca
     1681 gcatgctggg tgaagctttc gccgatgaat ggctggcata ttaccagtac tggctgggcg
     1741 ccaagatcgt caaaggtccg atgaaagatg ccgtggcagc tgagctgctc ctgcacgcca
     1801 ccgaagagct ggctcacgcc gatttgctgg ccctgcgcat catccagctg ggcggcaccc
     1861 cgccgatcag ccccgcagcc tggctcgcga ccacccattg cggttatgcc gctcccaccg
     1921 atcccttcgt caaacagatc ctggcgcaga atatcgccgg cgagcagtgt gctatcgatg
     1981 tttaccagaa tctgctgaaa atcgttcgag agccggaccc ggtaacctac aaccttatcc
     2041 tcacgatcct ccagcaagag gtcgagcacg aagaagacct gcaggcgctg gacgaggacc
     2101 tcgaatcgct gatgatgcgc agcctgtaag atagcgtacc gccggttggc agaaaaacta
     2161 accggcggtt atatttattg accccacttt aagttatacc tcctcccaca ccacaccttt
     2221 aaatttcttc ctcgccgctt ccaccccctc ctccttcagc gtcttctcca cct
//
LOCUS       DATMXK010000586         2766 bp    DNA     linear   ENV 31-JAN-2024
DEFINITION  MAG TPA_asm: Anaerolineales bacterium isolate SMAG_U13118
            k141_42935, whole genome shotgun sequence.
ACCESSION   DATMXK010000586 DATMXK010000000
VERSION     DATMXK010000586.1
DBLINK      BioProject: PRJNA983538
            BioSample: SAMN37943715
            Sequence Read Archive: SRR7629279
KEYWORDS    WGS; ENV; Metagenome Assembled Genome; MAG; Third Party Data; TPA;
            TPA:assembly.
SOURCE      Anaerolineales bacterium (soil metagenome)
  ORGANISM  Anaerolineales bacterium
            Bacteria; Chloroflexota; Anaerolineae; Anaerolineales.
REFERENCE   1  (bases 1 to 2766)
  AUTHORS   Ma,B., Lu,C., Wang,Y., Yu,J., Zhao,K., Xue,R., Ren,H., Lv,X.,
            Pan,R., Zhang,J., Zhu,Y. and Xu,J.
  TITLE     A genomic catalogue of soil microbiomes boosts mining of
            biodiversity and genetic resources
  JOURNAL   Nat Commun 14 (1), 7318 (2023)
   PUBMED   37951952
  REMARK    Erratum:[Nat Commun. 2023 Dec 6;14(1):8079. PMID: 38057423]
            Publication Status: Online-Only
REFERENCE   2  (bases 1 to 2766)
  AUTHORS   Caiyu,L.
  TITLE     Direct Submission
  JOURNAL   Submitted (26-OCT-2023) Soil Science, Zhejiang University, Jianshe
            Three Road 733, Hangzhou, Zhejiang 311200, China
COMMENT     The annotation was added by the NCBI Prokaryotic Genome Annotation
            Pipeline (PGAP). Information about PGAP can be found here:
            https://www.ncbi.nlm.nih.gov/genome/annotation_prok/
            
            ##Genome-Assembly-Data-START##
            Assembly Method        :: Megahit v. 1.2.9; Metawrap v. 1.2.1
            Genome Representation  :: Full
            Expected Final Version :: Yes
            Genome Coverage        :: Not Applicable
            Sequencing Technology  :: Illumina HiSeq
            ##Genome-Assembly-Data-END##
            
            ##Genome-Annotation-Data-START##
            Annotation Provider               :: NCBI
            Annotation Date                   :: 01/05/2024 21:30:51
            Annotation Pipeline               :: NCBI Prokaryotic Genome
                                                 Annotation Pipeline (PGAP)
            Annotation Method                 :: Best-placed reference protein
                                                 set; GeneMarkS-2+
            Annotation Software revision      :: 6.6
            Features Annotated                :: Gene; CDS; rRNA; tRNA; ncRNA
            Genes (total)                     :: 2,137
            CDSs (total)                      :: 2,121
            Genes (coding)                    :: 2,114
            CDSs (with protein)               :: 2,114
            Genes (RNA)                       :: 16
            tRNAs                             :: 14
            ncRNAs                            :: 2
            Pseudo Genes (total)              :: 7
            CDSs (without protein)            :: 7
            Pseudo Genes (ambiguous residues) :: 0 of 7
            Pseudo Genes (frameshifted)       :: 1 of 7
            Pseudo Genes (incomplete)         :: 5 of 7
            Pseudo Genes (internal stop)      :: 3 of 7
            Pseudo Genes (multiple problems)  :: 1 of 7
            ##Genome-Annotation-Data-END##
FEATURES             Location/Qualifiers
     source          1..2766
                     /organism="Anaerolineales bacterium"
                     /mol_type="genomic DNA"
                     /submitter_seqid="k141_42935"
                     /isolate="SMAG_U13118"
                     /isolation_source="soil"
                     /db_xref="taxon:2073117"
                     /environmental_sample
                     /country="USA"
                     /lat_lon="44.9564628 N 92.9913765 W"
                     /metagenome_source="soil metagenome"
                     /note="metagenomic"
     gene            40..2610
                     /locus_tag="VN363_10685"
     CDS             40..2610
                     /locus_tag="VN363_10685"
                     /inference="COORDINATES: protein motif:HMM:NF013491.4"
                     /note="Derived by automated computational analysis using
                     gene prediction method: Protein Homology.
                     GO_function: GO:0005524 - ATP binding [Evidence IEA];
                     GO_function: GO:0016301 - kinase activity [Evidence IEA];
                     GO_process: GO:0016310 - phosphorylation [Evidence IEA]"
                     /codon_start=1
                     /transl_table=11
                     /product="PEP/pyruvate-binding domain-containing protein"
                     /protein_id="HWQ85025.1"
                     /translation="MYDQKSPLILPLNDASATLELTGGKGASLARLAAAGLPVPPGFH
                     VTTTAYRCFVNENELQEKILAAVATVSADQPATFAEASRQIEILFANSVMPEAVNQGI
                     RQAYALLGGGKISVAVRSSATAEDLPGMSFAGQQDTYLNMRGEEMVLGAVQRCWASLW
                     TARAIDYRTHYNIAPEDVSLAVVIQKLVPADAAGILFTANPVSGARDQVVINAAWGLG
                     EAIVSGKVNPDTMVVDKISWKVIEQNINKKEVMTVRTPEGTHEEVVQADQSARAVLSS
                     AQAVELARLGVKIEALYGQPMDIEWAIHNGRIFIVQARPITTLHEHNSKNLEWNDSLG
                     GDYLWTNGNLGEAIPDVMTPCTWSLVQIFMSDAMATSSVAPYFGYGNIGGRFYMNLSV
                     AASLSAAFGINRKRFAKLTEEVFGRLPEGLEIPLIPLSRWSVLGKLLPVAARLLRRTR
                     ANQKRLPDFLAAAQERCETLRAKIQTTPNAENLITLWQSEVLPFFHDCCYMLEAAGRQ
                     DGGALVSVRSKLKKMVGEADTNTLLTGVGTETNQLASLGPLIGLTQLAHGEIDRATFA
                     RKYGHRSSHEFEVSIARPAEDTDWIDKQLVGLNEAKADVTVLLQRQESARVVAWERFR
                     ARYPKKEAAVRSQIERWSVVARNREVARTEVIRVFWVLRAFALRASELTGQGEDIFFL
                     SIDEILALLSGDNSSVANIPDRRSTYAYYCTLPAYPALILGHFDPSRWAADPERRSDL
                     FDARGISVAKNPGIIGFAGAAGVVEGRVRVIFSVEDGEQLQPGEILVTTVTNVGWTPL
                     FPRAAAVVTDVGAPLSHAAIVARELGIPAVVGCGNATMRLHTGDLVRVNGMQGTVEVL
                     "
BASE COUNT          641 a          679 c          813 g          633 t
ORIGIN      
        1 gaattacaat gatgattggt aaagagagga gagttcgtta tgtatgatca aaaatcccct
       61 ctaattctgc ccctgaatga tgcctccgcc acgctagaac tgactggtgg gaaaggagct
      121 tcgttagctc gtctagccgc ggcagggcta ccggttcccc ctggttttca tgtcaccacc
      181 acagcctatc gttgctttgt gaacgaaaat gagctgcaag agaagatttt ggcagcggta
      241 gctacggtct cagctgatca accggctact ttcgccgaag catctcggca gatcgaaata
      301 ctttttgcga acagtgtaat gccggaggca gttaaccagg ggattcgtca ggcttacgcg
      361 ctactcggtg ggggtaagat ctctgttgca gtacgttcct cagccaccgc cgaagacttg
      421 ccggggatgt cctttgcagg tcagcaggat acgtatctaa acatgcgcgg agaggaaatg
      481 gtactggggg cggtccagcg ttgctgggcc tcgctctgga cggcgcgcgc cattgactac
      541 agaacccact ataatattgc tccggaggat gttagcctag cggttgtcat ccaaaaacta
      601 gtgcctgccg atgccgctgg tatcttgttc actgccaatc cagtgagtgg tgctcgcgat
      661 caggtagtga tcaacgcagc ctgggggctg ggtgaggcta ttgttagcgg taaggtaaac
      721 cccgacacta tggtagtgga caagatcagt tggaaggtta ttgagcagaa tatcaacaag
      781 aaagaggtga tgaccgttcg tacccccgaa gggacacacg aggaagtggt tcaggccgat
      841 cagagtgcac gggcagtact cagttcagca caggcggtcg aacttgctcg ccttggtgtc
      901 aagatcgagg cgttatatgg ccagccgatg gatattgaat gggcgatcca caatggccga
      961 atcttcatcg tgcaggcccg ccccatcacc accctgcacg agcataactc gaagaacctg
     1021 gaatggaatg atagtcttgg cggcgactac ctatggacga atggcaacct cggcgaggcg
     1081 attccagacg tgatgacgcc ttgtacttgg tcactcgtcc agatattcat gtccgatgct
     1141 atggcgacct catctgttgc cccttacttt gggtatggca atatcggagg acggttctac
     1201 atgaacttga gcgtagcagc atcactttca gctgcgtttg gtatcaaccg aaagcgattc
     1261 gccaagttga ccgaggaagt cttcgggagg cttccggagg gtctggagat cccactcatc
     1321 ccgctgtcgc gctggagtgt gttgggtaag ttgctgccgg ttgctgctcg tctcttgcgc
     1381 cgaacacgtg ccaatcagaa gagactgcct gattttctcg cagcagcaca ggaacgatgc
     1441 gaaacattgc gtgccaaaat ccagaccacg cccaacgcag agaacctcat taccctatgg
     1501 cagagtgaag tgttgccttt cttccacgat tgctgttaca tgctggaggc tgccggcagg
     1561 caagatggag gtgcgctcgt atccgtccga agtaagctga agaagatggt gggtgaagca
     1621 gacactaaca ccttgctgac gggagtgggt actgagacaa atcagcttgc cagcctcggg
     1681 ccactaattg ggcttacaca attggcacac ggcgaaatcg accgtgcaac ctttgcccga
     1741 aagtacgggc atcgcagctc acatgagttc gaggtgtcca tcgctcgccc agccgaggac
     1801 acggattgga tcgacaagca gcttgttggt ctgaatgagg ctaaggcgga cgtcacagtg
     1861 cttcttcagc gccaggaaag cgcgcgggta gtcgcctggg agcgatttcg agcgcgttat
     1921 ccaaagaagg aagccgcagt acgcagtcag atcgagcgtt ggtcggtggt agctcgcaac
     1981 cgcgaagtag cgcgtacgga agtgattcgg gttttttggg tgctgcgtgc attcgctctc
     2041 cgtgcaagtg aactaactgg tcagggtgag gatatcttct tcctctccat cgacgagatt
     2101 ctggccttac tcagtggcga taattcatcc gtcgccaata tcccagatcg gcgtagtacc
     2161 tacgcttatt attgtacgct tccggcgtat ccggcactga tcctcggtca ctttgaccct
     2221 tcccggtggg ctgcggaccc agagcgacgc agtgatttat ttgatgcccg aggcattagc
     2281 gttgcaaaga acccggggat tataggtttt gctggcgcgg ctggagtagt ggaaggccgc
     2341 gtacgtgtga tattttcggt agaggatgga gaacagttgc agcccggtga aattttggta
     2401 acgaccgtaa ccaacgttgg atggacgccg ctcttcccga gagctgctgc agtggtgact
     2461 gatgttggag cgcccctctc gcacgctgcg atcgtagcac gtgagctcgg catacccgct
     2521 gtggtcggct gtggcaatgc aacgatgcgt ctgcacaccg gcgatctggt gcgggtaaat
     2581 ggtatgcagg gaacggttga agtattgtaa ggtggcagat gtcccacaat gtagcaactt
     2641 tacaaaattg tgaatacata actagaaaga gacataaacc gaaaaggtta atagtctttg
     2701 agaaactggc aatgaagtaa catcgacgca cgtggagacg ctggtagtgc tatagcgggg
     2761 tgggat
//
